LOCUS       DASJSZ010000001        88664 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.001, whole genome shotgun sequence.
ACCESSION   DASJSZ010000001 DASJSZ010000000
VERSION     DASJSZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 88664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.001"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            40..1164
                     /locus_tag="U4B03_000001"
     CDS             40..1164
                     /locus_tag="U4B03_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2066883.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            1391..2308
                     /locus_tag="U4B03_000002"
     CDS             1391..2308
                     /locus_tag="U4B03_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2066884.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            2301..3134
                     /locus_tag="U4B03_000003"
     CDS             2301..3134
                     /locus_tag="U4B03_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2066885.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            3217..4335
                     /locus_tag="U4B03_000004"
     CDS             3217..4335
                     /locus_tag="U4B03_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2066886.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            4329..6227
                     /locus_tag="U4B03_000005"
     CDS             4329..6227
                     /locus_tag="U4B03_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEN2066887.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            6221..7444
                     /locus_tag="U4B03_000006"
     CDS             6221..7444
                     /locus_tag="U4B03_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2066888.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7530..9464)
                     /locus_tag="U4B03_000007"
     CDS             complement(7530..9464)
                     /locus_tag="U4B03_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2066889.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            9690..10346
                     /locus_tag="U4B03_000008"
     CDS             9690..10346
                     /locus_tag="U4B03_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN2066890.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(10420..10773)
                     /locus_tag="U4B03_000009"
     CDS             complement(10420..10773)
                     /locus_tag="U4B03_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN2066891.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            10965..11894
                     /locus_tag="U4B03_000010"
     CDS             10965..11894
                     /locus_tag="U4B03_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEN2066892.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            11905..12741
                     /locus_tag="U4B03_000011"
     CDS             11905..12741
                     /locus_tag="U4B03_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEN2066893.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            12989..13765
                     /locus_tag="U4B03_000012"
     CDS             12989..13765
                     /locus_tag="U4B03_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2066894.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            13941..14684
                     /locus_tag="U4B03_000013"
     CDS             13941..14684
                     /locus_tag="U4B03_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2066895.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            14677..16257
                     /locus_tag="U4B03_000014"
     CDS             14677..16257
                     /locus_tag="U4B03_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEN2066896.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(16508..16987)
                     /locus_tag="U4B03_000015"
     CDS             complement(16508..16987)
                     /locus_tag="U4B03_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN2066897.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(17003..17755)
                     /locus_tag="U4B03_000016"
     CDS             complement(17003..17755)
                     /locus_tag="U4B03_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN2066898.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            17872..17942
                     /locus_tag="U4B03_000017"
     tRNA            17872..17942
                     /locus_tag="U4B03_000017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17904..17906,aa:Gly,seq:ccc)
     gene            18303..19028
                     /locus_tag="U4B03_000018"
     CDS             18303..19028
                     /locus_tag="U4B03_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN2066899.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            19283..19549
                     /locus_tag="U4B03_000019"
     CDS             19283..19549
                     /locus_tag="U4B03_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEN2066900.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            19546..19977
                     /gene="ruvX"
                     /locus_tag="U4B03_000020"
     CDS             19546..19977
                     /gene="ruvX"
                     /locus_tag="U4B03_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEN2066901.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            20002..20307
                     /locus_tag="U4B03_000021"
     CDS             20002..20307
                     /locus_tag="U4B03_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEN2066902.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(20388..21533)
                     /locus_tag="U4B03_000022"
     CDS             complement(20388..21533)
                     /locus_tag="U4B03_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2066903.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(21594..22241)
                     /locus_tag="U4B03_000023"
     CDS             complement(21594..22241)
                     /locus_tag="U4B03_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2066904.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            22435..22728
                     /locus_tag="U4B03_000024"
     CDS             22435..22728
                     /locus_tag="U4B03_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2066905.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            22772..23083
                     /locus_tag="U4B03_000025"
     CDS             22772..23083
                     /locus_tag="U4B03_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2066906.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            23080..23704
                     /locus_tag="U4B03_000026"
                     /pseudo
     CDS             23080..23704
                     /locus_tag="U4B03_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            23845..24003
                     /locus_tag="U4B03_000027"
     CDS             23845..24003
                     /locus_tag="U4B03_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2066907.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            24004..24366
                     /locus_tag="U4B03_000028"
     CDS             24004..24366
                     /locus_tag="U4B03_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2066908.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            24403..25251
                     /locus_tag="U4B03_000029"
     CDS             24403..25251
                     /locus_tag="U4B03_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEN2066909.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            25241..26716
                     /locus_tag="U4B03_000030"
     CDS             25241..26716
                     /locus_tag="U4B03_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEN2066910.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            26982..27389
                     /locus_tag="U4B03_000031"
     CDS             26982..27389
                     /locus_tag="U4B03_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEN2066911.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            27392..27607
                     /locus_tag="U4B03_000032"
     CDS             27392..27607
                     /locus_tag="U4B03_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2066912.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            27611..27991
                     /locus_tag="U4B03_000033"
     CDS             27611..27991
                     /locus_tag="U4B03_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2066913.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            28125..28280
                     /locus_tag="U4B03_000034"
     CDS             28125..28280
                     /locus_tag="U4B03_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEN2066914.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            28349..28822
                     /locus_tag="U4B03_000035"
     CDS             28349..28822
                     /locus_tag="U4B03_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEN2066915.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            28819..30513
                     /locus_tag="U4B03_000036"
     CDS             28819..30513
                     /locus_tag="U4B03_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2066916.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            30479..30664
                     /locus_tag="U4B03_000037"
     CDS             30479..30664
                     /locus_tag="U4B03_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2066917.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            30668..31843
                     /locus_tag="U4B03_000038"
     CDS             30668..31843
                     /locus_tag="U4B03_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2066918.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            31836..33359
                     /locus_tag="U4B03_000039"
     CDS             31836..33359
                     /locus_tag="U4B03_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2066919.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            33415..33699
                     /locus_tag="U4B03_000040"
     CDS             33415..33699
                     /locus_tag="U4B03_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN2066920.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            33686..34021
                     /locus_tag="U4B03_000041"
     CDS             33686..34021
                     /locus_tag="U4B03_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2066921.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            34316..34942
                     /locus_tag="U4B03_000042"
     CDS             34316..34942
                     /locus_tag="U4B03_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2066922.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            35684..36520
                     /locus_tag="U4B03_000043"
     CDS             35684..36520
                     /locus_tag="U4B03_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN2066923.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            36584..37015
                     /locus_tag="U4B03_000044"
     CDS             36584..37015
                     /locus_tag="U4B03_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2066924.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(37077..38399)
                     /locus_tag="U4B03_000045"
     CDS             complement(37077..38399)
                     /locus_tag="U4B03_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEN2066925.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(38553..40193)
                     /locus_tag="U4B03_000046"
     CDS             complement(38553..40193)
                     /locus_tag="U4B03_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN2066926.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            40526..40996
                     /locus_tag="U4B03_000047"
     CDS             40526..40996
                     /locus_tag="U4B03_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEN2066927.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            41218..42321
                     /locus_tag="U4B03_000048"
     CDS             41218..42321
                     /locus_tag="U4B03_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN2066928.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            42605..43285
                     /locus_tag="U4B03_000049"
     CDS             42605..43285
                     /locus_tag="U4B03_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2066929.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            43375..44409
                     /locus_tag="U4B03_000050"
     CDS             43375..44409
                     /locus_tag="U4B03_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN2066930.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            44423..44758
                     /locus_tag="U4B03_000051"
     CDS             44423..44758
                     /locus_tag="U4B03_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2066931.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            44742..47222
                     /locus_tag="U4B03_000052"
     CDS             44742..47222
                     /locus_tag="U4B03_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2066932.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            47278..48837
                     /locus_tag="U4B03_000053"
     CDS             47278..48837
                     /locus_tag="U4B03_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2066933.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            48834..50198
                     /locus_tag="U4B03_000054"
     CDS             48834..50198
                     /locus_tag="U4B03_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2066934.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            50369..51439
                     /locus_tag="U4B03_000055"
     CDS             50369..51439
                     /locus_tag="U4B03_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2066935.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            51454..52122
                     /locus_tag="U4B03_000056"
     CDS             51454..52122
                     /locus_tag="U4B03_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2066936.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            52142..52720
                     /locus_tag="U4B03_000057"
     CDS             52142..52720
                     /locus_tag="U4B03_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2066937.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            53121..54032
                     /locus_tag="U4B03_000058"
     CDS             53121..54032
                     /locus_tag="U4B03_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2066938.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(54410..55327)
                     /locus_tag="U4B03_000059"
     CDS             complement(54410..55327)
                     /locus_tag="U4B03_000059"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEN2066939.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            55529..55966
                     /gene="zapA"
                     /locus_tag="U4B03_000060"
     CDS             55529..55966
                     /gene="zapA"
                     /locus_tag="U4B03_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEN2066940.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            56024..56572
                     /locus_tag="U4B03_000061"
     CDS             56024..56572
                     /locus_tag="U4B03_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEN2066941.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            56687..59047
                     /locus_tag="U4B03_000062"
     CDS             56687..59047
                     /locus_tag="U4B03_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN2066942.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            59134..59448
                     /gene="trxA"
                     /locus_tag="U4B03_000063"
     CDS             59134..59448
                     /gene="trxA"
                     /locus_tag="U4B03_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEN2066943.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            59630..61408
                     /gene="uvrC"
                     /locus_tag="U4B03_000064"
     CDS             59630..61408
                     /gene="uvrC"
                     /locus_tag="U4B03_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEN2066944.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            61553..62452
                     /locus_tag="U4B03_000065"
     CDS             61553..62452
                     /locus_tag="U4B03_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2066945.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            62445..63707
                     /locus_tag="U4B03_000066"
     CDS             62445..63707
                     /locus_tag="U4B03_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2066946.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            63868..65766
                     /locus_tag="U4B03_000067"
     CDS             63868..65766
                     /locus_tag="U4B03_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEN2066947.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(66096..66815)
                     /locus_tag="U4B03_000068"
     CDS             complement(66096..66815)
                     /locus_tag="U4B03_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEN2066948.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            67152..67484
                     /locus_tag="U4B03_000069"
     CDS             67152..67484
                     /locus_tag="U4B03_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2066949.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            67471..69474
                     /locus_tag="U4B03_000070"
     CDS             67471..69474
                     /locus_tag="U4B03_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEN2066950.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            69493..69963
                     /locus_tag="U4B03_000071"
     CDS             69493..69963
                     /locus_tag="U4B03_000071"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEN2066951.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            69984..70568
                     /locus_tag="U4B03_000072"
     CDS             69984..70568
                     /locus_tag="U4B03_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEN2066952.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            70583..71569
                     /locus_tag="U4B03_000073"
     CDS             70583..71569
                     /locus_tag="U4B03_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEN2066953.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            71559..71870
                     /locus_tag="U4B03_000074"
     CDS             71559..71870
                     /locus_tag="U4B03_000074"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEN2066954.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            71892..73673
                     /locus_tag="U4B03_000075"
     CDS             71892..73673
                     /locus_tag="U4B03_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEN2066955.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            73666..75042
                     /locus_tag="U4B03_000076"
     CDS             73666..75042
                     /locus_tag="U4B03_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEN2066956.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            75049..75684
                     /locus_tag="U4B03_000077"
     CDS             75049..75684
                     /locus_tag="U4B03_000077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEN2066957.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            75714..77069
                     /locus_tag="U4B03_000078"
     CDS             75714..77069
                     /locus_tag="U4B03_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEN2066958.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(77261..78655)
                     /locus_tag="U4B03_000079"
     CDS             complement(77261..78655)
                     /locus_tag="U4B03_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN2066959.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            78747..79718
                     /locus_tag="U4B03_000080"
     CDS             78747..79718
                     /locus_tag="U4B03_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN2066960.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            79715..80497
                     /locus_tag="U4B03_000081"
     CDS             79715..80497
                     /locus_tag="U4B03_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN2066961.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(80666..81037)
                     /locus_tag="U4B03_000082"
     CDS             complement(80666..81037)
                     /locus_tag="U4B03_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2066962.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            81171..81614
                     /locus_tag="U4B03_000083"
     CDS             81171..81614
                     /locus_tag="U4B03_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEN2066963.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(81657..82466)
                     /gene="yidA"
                     /locus_tag="U4B03_000084"
     CDS             complement(81657..82466)
                     /gene="yidA"
                     /locus_tag="U4B03_000084"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEN2066964.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(82485..83852)
                     /locus_tag="U4B03_000085"
     CDS             complement(82485..83852)
                     /locus_tag="U4B03_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2066965.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(83836..84666)
                     /locus_tag="U4B03_000086"
     CDS             complement(83836..84666)
                     /locus_tag="U4B03_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEN2066966.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            84829..85266
                     /locus_tag="U4B03_000087"
     CDS             84829..85266
                     /locus_tag="U4B03_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEN2066967.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            85342..85962
                     /locus_tag="U4B03_000088"
     CDS             85342..85962
                     /locus_tag="U4B03_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEN2066968.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            86151..86657
                     /locus_tag="U4B03_000089"
     CDS             86151..86657
                     /locus_tag="U4B03_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEN2066969.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            86674..87573
                     /locus_tag="U4B03_000090"
     CDS             86674..87573
                     /locus_tag="U4B03_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEN2066970.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            87660..88544
                     /locus_tag="U4B03_000091"
     CDS             87660..88544
                     /locus_tag="U4B03_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN2066971.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        29306 a        15041 c        18357 g        25960 t
ORIGIN      
        1 cgttttatat aagaacttga aaagaaagaa gggatagaca tgaaaaaaag atacaaattg
       61 gtgatagctt tgctaaacat taccctatta ttcagcttaa gtgcatgtgg aaaaactaac
      121 gaagaagtaa cagcaaacat cgcagatcca atgataaaaa aagccactac attagaagta
      181 tatgtcgcaa acggcaaact gacaaactat gttgaaaatg ctgcaaaaat ctataaccgc
      241 aaaactggag caaatattga cttaaaaatt gtgaatgtag cttcgggaac agcaacagta
      301 caaatgatca ccccaaaatt ggtaacacac gaagatatgc cggatattat ctccatggca
      361 gacacttcag cagcaggtgt gctggaaaaa tttcaagatg ctttttatag tgctgatgaa
      421 tatggatttt atgacaaata tggcgatcag ttttatgatc aaaaattgaa tattttgcgt
      481 caacaaacga gtaatgaaac gattattcct tgggctagtg attttacccc agcactttgt
      541 tattatcaac caaaaaaatt tgctgaaata ggtgtagatt ttgaagaaat cagttcttgg
      601 gaagaatata tccaggtggc aaaagcatta aaagaaaaaa ctggtagttt tggaattgcc
      661 ttgccagaat ctggggacca agaattgttt atcaacatgc tagcccagca gaatcaatct
      721 ttgcttgatg aagaaggaaa tatccgtctg aatactaaag aagcgaaaca tgctgcaaag
      781 ttaatcaaag aaatgatcga ctcagatatt gtacatttct acggtgcaca agatgctgag
      841 aaagcatttc aagaatctgc aatgtttgtt gctggcggct ggtatgctac gaatatgtct
      901 ttgaattttc ctgatgcatc tgacaaatgg cgcatggctc cactgattcc tttttcaaaa
      961 gaaaatccag gaaaaggtgc agtatcagga ggatcaagtt tttatgtccc taaagatgca
     1021 aaaaatccac aagtggcaca acaatttctt acatttatgc tgacagatga agaatgctta
     1081 gccaacgcac tggagctggg cgtggctaca tctaactcat tggcctatca aacagaagca
     1141 gctgagaaaa agtttgacta ctaaggagga caaaaatact atgaactatt gaataaaatc
     1201 aatgaaaata catcaaatac tgttttacca gccacttatt cagatgctac gtcttatttg
     1261 actaccgctg catataacta ttggcaaaca ggtgattttg aaaattcata tctaaaagaa
     1321 gcagaaaatt ttgctcataa gtacggcgta aaattaactg gtgattaaaa aatattagga
     1381 ggacttaacg atgatgcaat cttcgttacc aagcaagaca ataaagaaca gcaagaaaaa
     1441 agcaacagat acgtctcata aaaaaagtgc ttatggattg gtcacacctt ttgttatttt
     1501 ctttattgtg acgattttga ttcccatcat tatctcgttt gcttttagtt ttatgaaaat
     1561 tggctactca tttgaatttg ttggatttga caattatcta gctatcttcc gagatccatt
     1621 gtttattaaa tcttacacga atgttttgat tatcatggca ggatcaattc caataacgat
     1681 gctattagct atttactttg ctgttttatt gaattcagca cagttaaaag gaaaaggatt
     1741 ttttagaact gtctattata ttccaaccat tacatcaacg gtggctgtag cttccgtatt
     1801 catgactttt ttcaatccga ctgggttatt caatggattt ttgaatgcgc taggaattaa
     1861 cggtgtacag tggttaacaa atcctttttg gatacgaata tcaatgatca tcacgatgat
     1921 ttggcttagt gtaggatata acactatttt atttttagct ggtcttcaag ggatttctaa
     1981 agagatatac gaaagtgctg aaatagatgg agcatcgaaa tttcggcaat tctttttatt
     2041 aacgatcccc atgctaaagc caatcatttt aatggccaca gttttagcca ctatcaatgg
     2101 cttgggtaat ttcaatatcc caaatatatt ctttgggact tccaatggac cagaaaattc
     2161 agctttggtc gtaggagtca atttatataa aacaagtttt gaaatggttg attttggaaa
     2221 agcctctgct attgcctgga cgatggtgtt tgtatctgtt atattatcgg ctattcaatt
     2281 taaattcgga ggtgataaaa atgactaaac aaaagataca aagcagaatt tttacgtacg
     2341 tcattattgt gtttggtgcc ttaatatttt tgatgccata tttatatatg attgtagctt
     2401 caacgcaaag taatcaagaa attttaagtg gcggaatcaa tttcaagttt ggggatatgc
     2461 tattaaaaaa ctggcaagcc atcatggcga gatataatta tcccttgatt atttggaatt
     2521 cgattgttat tgctgtaatc agtacagttt tagctacagc cagttctaca atggcgggtt
     2581 atgcattagc aaagtatcgt tttatgggga ataaactgtt gttcaataca gttatgctat
     2641 ctaggatggt gccttttttt accacattga ttcctttatt ttacattatg agtcgtttag
     2701 gattatcgaa tacttatgca ggtgtgatca tcccttcgat tgccagtaca acttcagtgt
     2761 ttatgatgcg acaatatagc agtcaatttc ctacagcact gatggaggct gccagaatcg
     2821 atggtgccaa tgagtgggtg atttttacga aaatcgctgt accgatttta tcacctacga
     2881 ttattactag tggtttactt atctttatgg gaagttggaa tcaatatcta tttccattag
     2941 tcatgctttc agataaagat aaatatacgg ttcccttaat tatcaaaaat atgacggttt
     3001 ctagtacagg tgagccaatt aattatggcg caattatgct aattttagca acttcagtga
     3061 tcccaatggt cttgatttat gtgtgggcgc agtctaaatt taaggagaat gacattggag
     3121 ctgcaaataa gtaaaaaaaa taacgaatgt taaccatcta gttcgttcta ggagcaactc
     3181 cacattcata aaaattaaaa gaatggcggg atatccatga atgaacagac gaaggaatgg
     3241 tcaagaaaaa ttttgaatgt agcttctgaa aagatagatg cggaattaga cagaatgggg
     3301 aataaaattc catatatttc ttacaacgga aaatatacag acgtaataaa ggacagagga
     3361 atcgactggt ggaccaatgg tttttggagt ggtattttat ggcagctctt tcacaagaca
     3421 aaacgagaaa agttcaaaga ggcagcaata caacaagaac aattattaga atctgccctg
     3481 taccattttt cagacgttca tcatgatgta ggttttttat tcttaccatc ggctattgca
     3541 cattatcaac agtctcaaaa caaacatagt cgacaactag ggttacatgc agctacggta
     3601 cttgctggac gctataatat ttcagggaac tatttagtag catggaatga ccataaacct
     3661 ggttggatga ttattgatag tatgatgaat ttaccattac tgtattgggc ttcgagggaa
     3721 actggtgatc cgaggttctc taaaattgct cggcagcatg cagatacaac agctaagtat
     3781 ctagtgagga aagatggatc aaccgggcat atcgcagaat ttgatgcaga atcagggaaa
     3841 tatattcggt tacttggcgg gcaaggatac aatgagaatt cttgttggag cagaggaaat
     3901 gcatgggctt tatatggatt cgcgttaagt tatcgttaca caaaaaatcc gtactatcta
     3961 gctatagcag aaaagatagc agtaaatttt attcagaaaa tcgaaaaaac tggttatatt
     4021 cctctcattg attttgctgt aaaagattcg aatattgata ccagtgctgg agtatgcgct
     4081 gcatgcggtc ttctggaatt agcaggatat gtttcctctg tacgatcaaa aatgtatcat
     4141 gaagctgcta tcaatatact ccaaaagaca gaaaagcact atgcaaattg gaatatggat
     4201 gaagatggga tcattggagg cggagcagaa gcataccata gagaactgac ttataatgtg
     4261 ccactgatct attctgatta tttttttatt gaaggcttac ttcgattaga acagtcggaa
     4321 ttagctattt ggtagtaaaa agaggagaaa atacaatgaa acatccagta ccactattaa
     4381 ggaccagaca ggattacttt gacttattta aaaagataaa tcaaccactc cagccgtttt
     4441 atcgagcaag tggcgcacaa attgattata tacatcaagg ggtaggatat ggaaataaaa
     4501 ttgcaggaat ggaaggattc tctcgggttt tatggggagc ggggcccgca attgaccaat
     4561 tagatgaacg atggttgaaa ttaattctat caggaataaa agctggaaca gatccttccc
     4621 atcccgatta ttggggagaa atacatgaaa gagaccagcg tatggtagaa atgcccgcaa
     4681 tgttattagc gttatatcat catgaacaat tattatggaa aaaattagat acaggagaaa
     4741 aaagccagat tattcattgg ttctcgcaaa tttttgaata tgaatgtgca gatggtaatt
     4801 ggcaattttt taagataatt gtcggaacaa ttatccaaaa atttggttat ccagttaaag
     4861 aatcagcact gaaagaagca tttgataata tagaaaattg ttatcttcaa aatggctggt
     4921 atcgagacag ttcaagaggt cgccaagatt attataatcc ttttgcattt cattattatg
     4981 gtttgattta tagcgtacta gaaccagaaa agcccattag ccagatatat aagaaccgag
     5041 ctattgattt ttctagagac tatattcatt tttttgcgga agatggtgca aatgtgcctt
     5101 ttggtcgaag catgatttat cgttttgcag tttcaagctt ttggtcagct atgttgtggg
     5161 ccgatctttt gccttttaaa ttaggtgaaa tcaaggggtt agtcaatcgg aatataagat
     5221 ggtggatgca aaaagaaata tttgatgaaa aaggtatcct gaatattggt tatacctacc
     5281 ctcaattgac attgactgaa ccctataata gtacgttatc tccattatgg ctgaataaga
     5341 tttttttatt gttggcgcta cctgaagacc atgagtattg gacggctaaa gaaatgccta
     5401 tgcctattcg tgaaggaact aaacttcttt cagaagccaa tttgctcata caacatgata
     5461 atggacatac attttttttg aatgcgggac aattagggcc aaatttccat acattgacca
     5521 atgaaaaata tttaaaattt gcttattcta gtcaatttgg tttttcgata ccgcgagcga
     5581 atcaattgaa agcagaaatg gcaatggatt caatgctagg gattcagcga gcggacacat
     5641 atatcacgac cagttttaag aatgagagca tcaaaaccta tggacagttc atcgttcgaa
     5701 ataatgtacg agacattgta tgtaagaaag aggtacttgc ctctacatgg gaacctactt
     5761 tacaggcaaa aatcagaact tggcttattc cttttgaagg ctggcagatt cgcgtacaca
     5821 aagtagaatt agatacagaa tatgttctat atgagacagg gtttgctatt gaatgttctc
     5881 ctgagaaaga aggaataatc atagaagaag acaaggaaaa ctatagggtg agtgaagctg
     5941 gcttttcagg tattatctgt ttaagtgacg atactattaa acgaaaatcc acagctatta
     6001 tgggtttgcc caatacaaac ttgatgacat gggaaaacac ttttattcct ggattagagg
     6061 gtacatttgg aaaaggaacc cattggctcg gaacaggagt ggttgctcat caagatcggg
     6121 attatagttt agaagtatgg aacaatcgtc ccaaaattga ttttgatggg acaaccgggc
     6181 taactatcca aaataaaaac aataaaaaaa gactaaaatt atgctaaagg ctggtgggga
     6241 aatgcttgat aaagtgatgt ttgaagagat aatacatcca gaaaaatgga taaatgaatg
     6301 ttataaagaa ttaatggaaa aagaatggaa taatgagatc gagcgttgtc gcagacacac
     6361 ggagaggtta atcaaacgta tcagtaacag tcagtatcaa ttcccaagcg agtgtcctat
     6421 aaaaggacgt tatccattaa gagacaatat tggttggaca gaaggatttt ggacaggaat
     6481 taattggttg ttatttgaga agacgggtga tcctttattt caacaaacag ctgaaaaagc
     6541 agatatagat tttatccaca ggattgactg tcaaattatc actaatcatc acgatcttgg
     6601 ttttttgtat tctttatcaa ccgtagcaga ttataaaata acgggaaatc gaaaaatgaa
     6661 ggaagcttca attgatgctg cagaaacatt acttcagcgt ttttgggaga aggcaggagt
     6721 tatacaagct tggggagatc ctagtgatcc ttcacaacaa ggtagaatga tcatggattg
     6781 ttgcatgaat cttccattat tgtattgggc gagtgaagaa actggaaatc aaaaatataa
     6841 aaaagctgct caggctcata ttcaaaatgc agcagattat ttagtaagag aagatggttc
     6901 aacttatcat acattttata tggatgtcaa agatggaaaa ccaagatatg gaagtacgca
     6961 tcaaggttat agtgataatt cctgttgggc tagaggccaa gcatggggga tttacggttt
     7021 tgcattaagt tatcgttatt tgaaagaaca aaaatatttt gatttaagca aaatattagc
     7081 caattatttt atgaatcggt tgccagaaga taccatttgt tattgggatt tgatctttga
     7141 tgattctagc agagaagaaa aagatacatc tagtatggtg atcatggttt gtggattatt
     7201 agaattaata gaatcaggtc tattagacaa aacagaaaag aacttatacc agtctgctgt
     7261 ttggaaaatt atgcgacaat tgaaagaaag atatgctaca acggaagaag aagatggctt
     7321 tttaacacag tctgtttata ataagcccaa aaataatggg gtaaatacac cttgtatttg
     7381 gggtgattac tattatttag aagctttagt gagaatgact agtaattggt cttcttattg
     7441 gtgaaaaagg taaatatgga atttttgttt gataaaataa aaaaagaaaa accaaaacag
     7501 catgaatggt ttctgttttg gttttttcgt taattttcat attcttctaa ttccaagctt
     7561 agttcttccc attccgtcaa ttttgtttct tgatcttgtc tagcagcttc taaatcttgg
     7621 ttcagctcat tgagttttac gtgatcttcc aggttttctg gcgcgaccat ttcttgttcc
     7681 aactgatcga tccattcatc gatccgcgcc atttcttctt caacctgagt aatttttcgt
     7741 tgaaggctac ggataagctt ttgctgttct ttgctttggt aataatcaga tttgttcgtg
     7801 ggagcaggag cagattctgg ctcatcttgt gccaacaatt cggcgatttc ttcttcttcc
     7861 tgctttttct ctaaataata atcatagtct cctagataga ctttgctgcc attttcagag
     7921 agttcgacga cttttgtcgc gatccgattg atgaaatatc ggtcatggga aacaaaaagc
     7981 agtgtgccgt cataatcgat caatgcattt tctaatactt ctttgttgtc aatatctaaa
     8041 tggtttgttg gttcatccaa aatcaaaaaa ttttctttgt tcatcgctaa cttggctaaa
     8101 gcaacccgtg ctttttcacc gccacttagc agagggattg gtttttctac atcttcgcca
     8161 gaaaataaga aagcacctaa agcacttcgg atctcttttt ctggagtggt aggatgctca
     8221 tcccacaatt ctgctaggat cgtcttgttt gtatgcagat cagcttgacc ttgatcgtaa
     8281 tagccgatgg tgacatttgt ccctagcgtt tcttttccac ggataaaagg gatctggtga
     8341 atgattgatt tcagcaacgt cgatttcccg ataccattcg gacctactag agcaatcgct
     8401 tcttgacgac ggatatctag attgattggt tcagataagg tgacattatc atatccgata
     8461 gcggcatctt cgatttgcaa gactacattt ccagaaggct tttctatact gaatagaaag
     8521 tttgcttgtt tttcatcccc ttgaggacga tccagccggt ccattttttc taatgtcttt
     8581 ctgcggcttt gcgctcgttt tgtggtagag gcgcgaacaa ggttccgagc aacgaagtct
     8641 tctaatttat ggatttctgt ttgctgcttt tcataagcct tccattcact tgctaactgt
     8701 tctgctttca aatctagata tttcgaatag ttacctttgt aatggcgcat ttttcttctg
     8761 cttaattcat agacttcatt gactacttta tctaaaaaat aacggtcatg ggaaacaatc
     8821 aataatgcac cagtatagct ttgcagataa ttctcaagcc aggaaagtgt ctcgatatct
     8881 aaatggttcg ttggttcatc tagaatcaaa atgtcagggt gttgcaaaag catgcgagct
     8941 aaagctaaac gtgtgcgctg tccgcctgat aaagtatcga ttttctgatc ataaaatgaa
     9001 gaatcgaatt taaacccatg caatacggaa cgaatttcat tttcataacc atagccgttt
     9061 cgatctgaaa aatcgtgttg cagccggtcg tattctttta aataagaact ataagcaggt
     9121 gtgtctggcg taccttcgct aatgaaaatc tctagctcgc gcatccgctc ttccatttgt
     9181 ctaacttgtt caaaagcttt cagcatctcg ttccaaactg tttcattgga atcaagtcct
     9241 gtgtcctgag cgaggtatcc gagagtcgct gttttatttt tagcgatctt tccttcatca
     9301 ggtgcttcaa ttccagcaat gatctttaat aaagtcgatt ttccagcgcc atttctgcca
     9361 actaaagcga cacgagcgcc tgtactgatt tctaaatgta tgttttcaaa taagacttct
     9421 gcaccaaaat ggcgagcaat ctgattggct tgtaataata tcatggaatc ctcttttcta
     9481 ctttgtgaat tctcctcaaa taaaaaacta tttttcaatt agtagtctac cataaattcc
     9541 attatactgc gagatttgca gaaatgataa ataaatgaat aaagaataaa atttcacaat
     9601 atctgagcaa tggaattgat tttttcctat aaatactgct atgatggaga aatcaaagat
     9661 gtaattagac aatacgtgga ggtcatttcg tgaaagatca tattattcca aaggcgacag
     9721 ctaaaagact gcctttgtat tatcgctatt taaggatttt aaataatgct ggaaagaaaa
     9781 aagtgtcttc aactgaatta agtgaagccg ttcaagtaga tagtgcaacg atccgtcgag
     9841 atttttctta ttttggcgaa ttaggaaaac gtgggtatgg ttatgatgta gaagatctta
     9901 tgcacttttt tgcaaagaca ttaaacgacg atcaattgac aaatgttgcg ctgatcggtg
     9961 tagggaactt gggaagtgct ttactaaaat acaaattcca tcaaagtaat agcatacgta
    10021 tcagttgtgc ttttgatgtg aatgaggaaa tcgttgggcg catcgtagac ggtatccccg
    10081 tttatccaat ggaagatatg tcagaacaaa tcaagatcca acaaatcgaa gtagcaattt
    10141 taacgatacc cgctgaaaga gcacaagaag tagtcaatca gttaacggaa gcaggaatca
    10201 aaggaatctt gaactttacc gctgctcgat tgaatgctcc gtcagatgta ttgatccaaa
    10261 atgttgattt gacgaatgaa ttgcaaacct tgatctattt cttgcattct gacgcaaatg
    10321 cgcattccga aaatacagca gaataaaaat aaaaagcgat tgaacaaatg aaggcaaaac
    10381 tttcacttgt tcaatcgctt ttttctgttt ggacaacgat tattttgttc ggaagatttc
    10441 ttcataagaa agggaatcat cttctgctgt cagttcatct agtagataac ggattttttt
    10501 aaagtcgatg aaccgtgctt gccgaaagac ttcttttcct tgatgaaaaa tcaggacaac
    10561 tggcgcagtc agcacttcaa atttactagc tacttctggt gtttcaaatg catccaaatg
    10621 aagtgccgga atatcataat gtgtgagaag ttcctctagt cgtggacgaa cagcgtgaca
    10681 aacactgcaa ttaggcatcg aaagataaat gacggcaacg gatgactgtt gcagtatgtc
    10741 ttgaacttca gtcaagttag tggtttgttt catttttatt ctccctttta tgatagaagt
    10801 gatatgtatg agaccttaat ttaatgggaa taacagaaaa agtcaattga agtgctttgg
    10861 taaaaaataa gagttgtgta aaaatttaca gagcaacttg ttttttttga ataaaagcgt
    10921 atatttgcaa agcgaatgaa ctagaaataa aaaggaggaa actcatgttt tcttttattc
    10981 caaattcggt tttgcagatg agtacgattt tcttatcgat cgttattgaa gcattacctt
    11041 tcgtattatt aggctgtctt atctcaggcg ccttacaagt atttttgacc ccagaacgtg
    11101 tgaagagatg gcttcctgaa aatcgtatct tctctatttt tactggttcg atcttaggat
    11161 ttttctttcc ttcatgtgaa tgcggcatcg taccgatcgt tcatcaattt gttaaaaaag
    11221 gcgtaccagt tcacacagcg tttgctttta tgctgactgc tccgatcatc aatccaatcg
    11281 tgattttttc cacttttatc gcatttggca attcttggca gatggcaagc tggcgggtga
    11341 taggcagctt ttttgttgcc cttgtgattg gtgtctggct tgcctatttt caaaaagacg
    11401 ctgtattgaa agaaaaaatc acgtctaaag tagcagaaaa tcaccatcat catgtccatg
    11461 aagaagaaca aagtgagaag aatgaaccaa aaaatcagct gatccatcaa actgggcatg
    11521 tgttgacaca ttcaatcgac gaattttttg atacaggtcg ttatttgatc attggcggat
    11581 taatcgcagc tgcgatgcag acttatttac caactagaat tatgctaacg ctcggctcaa
    11641 caaagatttt agccattatc atcatgctgt tattggcctt tacgatgtct ctttgctcag
    11701 aagcagatgc gtttatagga tcttctttgt taagtctgtt cggcacagca cctgtagctg
    11761 cctttttggt ctttggccca atggtcgata tcaaaaatct actgatgatg aaacgatatt
    11821 ttacaggacg ctttatttta tcgctgatcg gactgattgc ccttagcgtt attggattca
    11881 cattatttat ttaggaggga aaatatgctg cggtttttga ttttatctgg gtatttcgaa
    11941 ttgatgatgt acttgcaagt ttctggcaaa ctcgatcaat atatcaatgt ccattatcgc
    12001 tacttagcta ttctttcaat gattttatct gctcttttgg ctttagttca gctgtatttg
    12061 tgggtcaaaa acggaaaagc agaagaaaag gcacacgagc acgatcatga ccatgggttg
    12121 tcaaaacctt atcaacgtgg aatcgcttat gtactgctag ctttgccagt aatcgtgggg
    12181 acgttgtttc caacagttag tctcgacact acgatcgtag aagccaaagg atttaatttc
    12241 ccagtaagta aagaatcagt aggggatccg gagatgcaga cacaatactt aaagccggat
    12301 accagtattt atttcaacaa atcagactac ttagaccaaa tgaatgagtt gaaagaaaag
    12361 tatggggata agcaaactat caagatcaca gatgaaaact atttagaagt tatggagcta
    12421 atttataatt accctagcga attcattggc aaaaaaatca gctatgaagg atttgtttac
    12481 caaacaccga acgatagcaa tacagatatc ttcttgttcc gattcggcat tatccattgt
    12541 gtggcagatt caggagtctt tggtttgttg acgcatatgc cggaaggagt aacggttaaa
    12601 aatgatgaat ggtataaaat agaaggaacc atccaatcag actattatca accattcaag
    12661 cgtgaaatcc catctgtagt tgttacaaac gctgagaaag tctctgctcc gaaaaatcag
    12721 tatgtgtatc gttcttttta aaagaacaaa ataaataaac atagctcaga cagctgacac
    12781 gcaaggacgt cggttgtctg gctttttttg catgatgtat tcatttgttt gatatttatt
    12841 ttagaagcag catcctgaaa gtatttgata tcggtttcaa aggtgttgga aaagattgtg
    12901 aagagaatta caatttattc ctaacatgta tgttgaaaag ttgtatgaaa tgattttgat
    12961 agtaaaatat cgagtggagg tactttttat ggtaacgcgt atatttattt tggatgatag
    13021 tttggttttt gaaaaaatgc tagtagaaat tttggaagaa tccagaattt tattaagtcc
    13081 ttggaaattt gagatcgttc agggattgaa cgttatacaa tttgttgaat tcattaaaaa
    13141 taatgaaatc tattcctcag atattttttt cctagacatc gatttgaacg cctattatag
    13201 cggtatcgaa ctaggcgaaa tgcttagaga aaagaatgaa aagtgtaaaa ttgtttatct
    13261 aacgtctcat ccgaataaag caataactgt tattaatcga aagattacgc ctagtggtta
    13321 tttgataaaa gaattcactt atgaaaaaaa taaaaaaagc atccaaagtt acttattgaa
    13381 agaaaaagaa aactatcctg ttcaacatgc tacgaaaaaa aggtggacaa ctgtccgtta
    13441 tggtaatagc gatcactata tccattacga tgaaatcctt tatctaatga gcatgccagg
    13501 ttataaaaat aaaataggcc tagtattgaa agatgaagag attctcgtaa acggcacaat
    13561 taaaaaatat aaaaaatcac tcgaatatga gtatttatgt aaagatttaa aagcttatat
    13621 cattaatact gaaaacatta catctattag cagagtaaac gaaatagtaa catttgcaaa
    13681 tggagaacaa ctagagatgg gaaagagaag tattgataaa ctaaagaatt ttatgcaaga
    13741 aaagatagaa aatgaatcat tttaaagaaa acaaagactc atatcttacc tgttagaaga
    13801 gtgttttcct gtagaagaga acaacccaag tagcagccta gtcgtttcta tccttttttg
    13861 ttatgcactc tttttgacat ttactccacc aataacgaag ggtacaataa aaagtaataa
    13921 caaacaaaag gagtacagaa atgattgaat tcatcaatgt aagtaaaagt tacgggaaaa
    13981 aacaagcatt aaaagacttg tctctctcga taagacaagg ggaaattttt ggatttttag
    14041 gacataatgg tgccggaaaa tcaactacga ttaaaagttt agttagtatt attgaaccat
    14101 catcgggaac gattttagta gatggaatga aattgacgga aaatcgattg agtatcaaac
    14161 aaaaaatcgg ttatgtgcct gattccccag atattttttt acaattgacg gctggagaat
    14221 attgggactt gatttcagct gcctatgaat tagacacaca aaaaaaagaa aagcgtttag
    14281 cagaattgac agcgcttttt gatatgtata gtcatcaaaa tgaaacaatc gcaagttttt
    14341 cacatggaat gcgtcaaaaa acgattttga tcggcacgtt gctgccagat ccagatattt
    14401 gggtattgga tgaacctttg caaggacttg atcctcaagc agcatttgat ttgaaagaaa
    14461 tgatgaaggc tcatgccgca aaaggaaaaa cagtcatttt ttctacacat gtattggata
    14521 ccgcacaaca gttatgcgat aaactggcta ttttgaagaa aggtgaattg atttatcagg
    14581 gggcagttag tgaattattg aatggctcac ctgatgaaac gctagaaaag atttatttga
    14641 aaatggctgg aaggcaagcg acaggagatg gaccgtatgt ataagcgaca attattagaa
    14701 ttaattcgag ttaatctttt atatgttaat ccacagctca cgaataaagc aagagagaaa
    14761 ggaaaaaagg gaaagcgatt aactatctat cttttgtctc aaaatctttt ttcagggctg
    14821 atttttttac tgatttacgg atttgctatg tttgctatga actttgttaa attgccaggt
    14881 ttctttactt attacgtggc actttttggt atgttaggtt taactcaaag tatatcagtc
    14941 atttataata tcttttttga aggaaatgac ctgcaagtat ttttgcctct gccttttgga
    15001 caagaacagg ttttttcagc aaagattttg gtagttgctt tgaccatcat tccttttgtt
    15061 tttccgcttc tagtcatgtt tattttaacc ggatggcgag ctgatctagg gattttgctt
    15121 actatcatta tctcaatcct tttgttttct ctatttttga ttattctttt ttgtctggct
    15181 agtttgattg tttttggttt ggctcgaaca gcagtgttta aaaaacatag aaaaacagtt
    15241 tctagtatct tgcttggcag ctcgatgctg atcgcatttg ctggaatcat gtggatgaat
    15301 catcaaacgg ggactattga aaatgagtta acagatcggc atccgatttc gatattattg
    15361 ccattctatg atgctgccag tcagccactt tctcaacaag gtttattggg ttggggaata
    15421 ctagctatca cggctctggg atttttcctt gctgtaaaac agctgatagt accaaagctt
    15481 acagagcaac tgactgattc aaacagtgag ttcaaaataa aaagaaccca taaaaagaac
    15541 caaaatgtgc atcagctttt atttagttac aatgctcaat tagtaaaaga acctaatttg
    15601 attatgcaag ttctttcttc ttccttgtta actccaatca tttttattct tgcttttgcc
    15661 ctaggaggcg aaataaaatt aactgattta gataataagt taatcggtgt tgttttcctg
    15721 gtagggatcg cattggcttt cttaacagtt aatcagactt cttttatcag caatcttatt
    15781 tctttggatc aagaaaattt tctgtttatc cgttctttgc caatctctat gaaccaatat
    15841 ttaaaagaaa agttcagatt cggatggata ttgcagagtt ttttaactgg tgggatcgca
    15901 ttattaagcg gactgtcttt tcagctgcct ttgtttttta tccctagctt gctatttggt
    15961 tctttattcg gctgctactt attgtcacta cgttatttcg ctagagatta tcggttgctg
    16021 ctgctgaact ggaccaatat taaccaatta tttaacagag gttcaggaag tttgggttta
    16081 gtcgcaacta tgatgggttc attgatcatc agtattattc ttatcgtctt atacggaatg
    16141 ttggctttgt tttatccatt ttggttagtg aatattcctg tgatcggttt agttctaata
    16201 ctttccgctc tatggatcgt ttattatcaa cgaacgtttt ggaaaaaatt tgaataaaga
    16261 agaggggccg tgacaaaaat catgtcgcag cccctttttt acagatagag ggtgttcaaa
    16321 agtcagtttc tcagtattaa gtcaatttta tgtaaaaatg tgagaagcta tttttacaaa
    16381 acttttttta atactcataa ttacttctta ttgctcctgc ggttacttgt cgcaatgttt
    16441 gtgagatatt ttgtcatcgc tcaaggctga acgtctcttt cacaaccttt tctttgttac
    16501 acttcgttta tttaaaaagc cgtttttttc gtttcatcat gattgcgtca cgctttactt
    16561 tgaaattacg gatgttatgg ttaaagactt ctgataatac aagacctaaa gcgattgctc
    16621 cagcaatcat aattgcttgg accgcataat gagccgcttc tcgataggat tcagtaacga
    16681 gatttcgaac cgcttgataa gctaaaccac cagggactag aggaaccatc ccgggaatat
    16741 tgaaaatcgt tacgggcatt ttcaaacgtt tagagaaaag atcgctgatg aatgctactc
    16801 ctaaagaacc aagcaatgaa ccaaaagcag cagatccgcc tatttgaaca cagatccagt
    16861 agatcatcca gccaaaagca ccagaaagac cacaacaaat caatgaacgc ctaggtacat
    16921 ttgtaataac ggcaaatgcc gcagtcgcta aaaaactaaa agaaaactga ataaatatcg
    16981 tccccatact tgtgttcctt tcctaataaa aaagctggaa aataaaagca atcgcaaaac
    17041 cgatcatcgt agcagtcatc atcgcttctg ttccgcgaga aactcctgag acataatgtc
    17101 cagctagcaa atcacggata gcgttcgtaa tttgaactcc aggaactaaa ggcatcacac
    17161 atccgataat gattagatcc tgattgcttc ctaagccaaa acgtacgctg tagatagcgg
    17221 ctatgccaat caataaagaa gataaaaatt ccgataaaaa tttgattcta aaaaatttca
    17281 aactatatag ataagctgcg tatcccaatc ctccaatgac taaagtcaca ggtaaatcgg
    17341 tcatctttcc gccaaataac accataattg ttccactgat cactgccgcg ctaacgattt
    17401 gcaaccagac aggaaagaaa gaacggtctc ggtcgattgc ttgcagttcg cagtaaagtt
    17461 cttctaatgt tagctctgaa gcaacatatt tacgtgaaag atcatttact tttactactt
    17521 tttctaaatt aatcgaacgg ttggtaattt gttccattcg gatggtcgag gtacgatcaa
    17581 gaccaataaa gagaccggtt tgagtgacat agctgaccaa acggaaattt ccagaagcta
    17641 aagcaattcg actcatcgta tcttctaccc gatacatctc cgcattgctt tccatcatga
    17701 ttttcccagc gagtaaacaa gtgtccagca acagatctaa ttcttttccc gtcatagcat
    17761 cttctccaat aattgattga ttagttttat tttactacaa aacataccag aagcgtaaag
    17821 aataaaaaaa cttgaggaag gaataaaaga ggcgtatact gtttttgtat tgcgggtgta
    17881 gtttagtggt aaaatcccag cttcccaagc tgatgtcgcg ggttcgattc ccgtcacccg
    17941 cttaaaatag gttgtgaaaa agctgctctg caacaagtaa taagaacagc caagcaaaaa
    18001 caacttctta tgtttttaac ttggatggac ttattaccgc agatgcaagc ttttgcgcac
    18061 cgtttaccaa gcggttgtga aatcagtgta ttgtcttgag tatgaagaaa atttttgaat
    18121 aataataaat accttacaga tagagtggtg actgattaga gacgaggaat ccgtctcttt
    18181 tttgtatttt taagccatct taaaacaggt tttatttttt gctgtttcta tttctttcta
    18241 gtatttattt ataggggaac atatgttaag atttagggag ctgttagtaa aggagtaaag
    18301 aaatgagcaa aaaaacaacc aaggcgatcg tggggatcgt cgctgctgtg attgcagggg
    18361 cattcggtgt ttccaccaca caaaaagatt cagatatctt tcagcagatt acaactattt
    18421 ttgattccac accacaagct tctcaagtag cggatgtgcc agaatatgat ggaaaacatc
    18481 aagaaataga aatcaatggg aatcagccgg aatttacaaa agaggatctt agcctttcca
    18541 aaggaacatg ggaaagctat tcaaatattg ataaattgaa cagagtagga caagccaatg
    18601 ctatgcttgg aaaagatttg ttccctactg aaaagagaga gcctttgtat attgacccga
    18661 caggttggaa acaaaagaaa ttaagtgacg ggcaatggtt atataatcgc agccatttaa
    18721 ttggtttcca gctgactggt caaaataaca acattaaaaa cttgatgaca gggacacgtt
    18781 cgttgaatgc tccatacatg cttgctcacg aaaatgatat tgcggcatat atcaaagaaa
    18841 caaatcatca tgtacgctac cgtgtcactc ctcattttga ggggaatgaa ctggtagcca
    18901 ggggagtgca attggaagca gaaagcattg aagacaaaaa aattgaattc aatgtcttta
    18961 tctacaatgt tcaagatggc tacacgatca attatgagac aggacaagca acaaaaacaa
    19021 aaaattagta gcagctattc gaatttttta tgaatattat gaaaaaattg tgtaaaaaca
    19081 tacattcaaa gaaatatagc gtatacttta tgaaaagagt acttttgata aagctacaga
    19141 aaaaaggaaa aaaacataca aatagtgttt cttaatttat tcggaagttt ggaatatttg
    19201 tttgtagttt tccctttctt cgagtaaaat aaggttgtag aaggtaagct ggaattacct
    19261 tgcgggaaag aggtgtagaa tcatgggttt tacagatgaa accgtacgtt ttggttttga
    19321 cgatagtcgc aaaaaagaag tgagcgaaac attagcgatt gtttatcggg ctttggaaga
    19381 aaaaggctac aatccaatca atcagattgt cggctacttg ctttctggag acccgtccta
    19441 cattcctcgt tatcgtgatg ctcgaaattt gattcgccgt cacgaacgcg atgagatcat
    19501 ggaagtaatt gtcaaggact acctctcaaa tcatggagtg aatttatgag agtaatgggc
    19561 ttggatgtcg gttctaagac agtaggtgtt gctatcagtg acccctttgg ctggacagct
    19621 caaggagtag agatcattcg gatcaatgaa gcagaagaag agtttggctt tgatcggttg
    19681 aaggaattag tagcacaata tgaagtagac cggtttgtag ttggattacc taaaaatatg
    19741 aacaattcca tcggaccaag ggcagaagct tccatgtcat acggagaaaa gatcaaagaa
    19801 ctattctcct tgccagttga ttatcaagat gaacgattaa ccactgttca ggcagaacgt
    19861 atgctagtag agcaggcaaa tacttcaaga gccaaacgaa aaaaagtcat cgataaatta
    19921 gcagctgtga tgatcttaca aaattattta gacggctcag gtaagcaatt attttaataa
    19981 taaaagaaaa gaggtaatct tatgacagaa cataaccatt cccacgacca tgaaggacac
    20041 gaacacatca cattggtgga tgaccaagga aatgaaacat tatatgaaat cctactaaca
    20101 gttgacggac aagaagaatt tggacgtaac tatgttcttt tatatcctgc tggtgtttca
    20161 gaagatgaag atgtagaatt acaagcttat gcatacatcg aaaacgaaga tggaacagaa
    20221 ggcgagttag aacaaatcga aacagacgca gaatgggaca tgattgaaga agtattcaat
    20281 acattcatgg cggaagaaga agaatagact ataaagctag gagaaacctt gatatgatag
    20341 ggtttctcct ttttttattt tttactgtat gccatattgt atgccatcta gtcaatattt
    20401 aagtgttgta ctaatttttc aacagtcttt tctttcgttt cttttgccaa gtgaccgtaa
    20461 caatcgatcg tctgactaat ttttgtatgc cgtaatctag tttgaacttc taccatagta
    20521 gctccagcgt ccaagagcat tgaagcatga gtgtgtcttg ctacatgcac accgagaaat
    20581 ggaaccatag ctttttcaca aatagtaatc aatcttgcat aaatagcgtg gtgtgtgatt
    20641 agttctttat agataccaca aaaaatcata gtcggatcag ttacaccaag tgtcagcata
    20701 tatttccgct gattaatctg ccattttttt aatatagaaa ttgttctttc atctagtgga
    20761 agaaccccag cactacaatc agttttagga tcactgattt tatagccatt tttggattgg
    20821 cttagtgttt tattgatggt gaccgtattt tcttgaaaat caatgtctga ccaattcaat
    20881 gcaagggctt cactgatcct aaccccgcta taaaagagaa agcgtagtaa cgttttatca
    20941 aagtcgtttc gataagtttc aggtttgctg tttaggtaat caaacaaggt gatcagttgt
    21001 tctttactgt aaactttgac cttttcttta tgcttagtgg tggttctttt aggcattaat
    21061 acgttgtcta gtggattagt ctcaataatc ccaagattga tagcgtagct aaaaatacgc
    21121 ttagaagttg aatgaagtac cttgtattgg tcagtagttt cagcccagct atttacaatt
    21181 tgttgggctt tttttaccgt gacttcattc acacgaagat ttccatattt tggaagaatc
    21241 cactttctaa acttgatttc agttttcatg aaggtggctt ctcggacagt ggttttatat
    21301 gtttctagcc acaagtcata aacttcttta aacgtttcgt tgttcgcttt tttaataccg
    21361 tttttatcgt agtccacttg caagcgtgac aaggcaagct gggcttcttt ttttgttttg
    21421 taacctcgtc tacgaacctt atcaggtttg ccagtcattt tgttcattcc aagataggct
    21481 tcatagagcc aaaatttacc ttttttagtt tcatattgct tgaatgttgc cataatagtt
    21541 ttccctccat agtgtagcaa gccgtggggg cggttggagg aggcagttta ttattatttt
    21601 gaatagtgtt tatttagctc agtaacaaga gctgtgaact cttttagaag atcattttca
    21661 taaaattctt gatactcttt tttctcatct gtattttttt ctgaatcatt aattaatgag
    21721 tcatatcgta aaagcgtatt tagaagagct gcgagataag caattggggt actcccgtca
    21781 ggaaattcaa ttcgagatcc tgttttaaga tattttattg cgtttgttaa ataaatcttc
    21841 tgtgagacag tcaggttatc ttcttcgatg ttattgatag cattagaaag attagaatca
    21901 attatagagt tatttttttc aatagttttt ttcaattgct tttcaagcac atttcgctta
    21961 atttcgggca tcatatgcat atctcttaat gtataagatc cgtttaatag ataggtagtg
    22021 gatacatcta atacctttga aagttgttta agcctggtgt tattgggaag attatagcca
    22081 ttttcccaat tactaacggc acctttactg gccggtgggg ataatttacc tccaagttgt
    22141 tccattgaga gtcccttttc aattctagcc agtctaattc tttgtcctgt ctccactgct
    22201 tcggggtatt tttttctagc cacattttca ccaccattca tatattttct ctactatatc
    22261 acgatttaaa tatgtgtgaa acataaaagt tcaaaaaagt atgttaaaat tcttgcaaat
    22321 gaaaaggtat gatggtatat tgagtttata agaggtatag aaatgtattc aagagcctct
    22381 tgcatttttt taaaatcttt gtatagaaat gtatgctaac gaggggggat aaatatggaa
    22441 ttaaaaattg acaaactcac gcttgatgat ccagctttta aaattattga cgatcgagtt
    22501 gttcaacaag tagagcaaac tcaaggaacg ttactttcat tattagaaaa aaagtaccag
    22561 tttccaaaat atatgaacaa ggcacaagct gctaagtata tgaacgtttc atacaatact
    22621 atgatgcaga aatatgttcc taacggttta aagttgatta tagttgatgg agtcgtaaga
    22681 atttcacaag atgaatgtga tcgctttatg gaagaatacc aaaaataaac gcaagccgtg
    22741 ggggcaaagg gattaatagg aggaaataat aatggaatcg acacaaaata aagcaatcga
    22801 aaaagtatta tcaacaattc ttacagaaga cacagcggca gaattagcaa acttggaagg
    22861 caaagcgcta gaagaaacgt ttgaatgttt gtacgaacaa atggactatc aaaaattgct
    22921 accacaagaa ccaacggtaa gcggagtatt gcgtggcttg aacgatttag tacaagcaga
    22981 gtttaaagaa cgactttcta ttgaagagta tcaagaaatt ttgtatcagc aagttgagtt
    23041 gttagcgagc ttgctagggc ttgaactgga ggacgttgaa tgaaaactga aatttggaac
    23101 ggacacatta taagatttgt cgatattaat gatgaatggt gggcagtggc gaaagatgtt
    23161 gcggaagcgt taggtcttaa acaggttact agagccattc actctttgcc taaagatggg
    23221 gttactacta gtaaggtcat cgattcattg ggcagaacac aagatgtaaa catcatcaac
    23281 gaaaaaaaat atttaccgca tggcattcaa aagccgtaaa aaagaagccg aggcatttca
    23341 agactggatt ttcgacatca tcaaagaact acgccagtcc acaggacttg aaggctttca
    23401 agtattccgc atgctagaca aagaacacca aaaagaagca atgactaaat taagccacgc
    23461 tattactgaa ccaaaaccag tagactacat taaagcgaat gtgatagcca ataaagctgt
    23521 atcaactatt tatggtcatt ccaaaatggt aaaaaagaaa gatatgactc ctgaaatgtt
    23581 ggttgatcgt gaacctattt tagatgagac agtagaatta atgactgtta aagaaaaata
    23641 tgggttgcag ttttcagtga gtgagaaaat ttataatcgt tctgctgaat tgcagacaac
    23701 ttaggagcat taacatgatc gagaacaaag aaaaagcctt atccataaca gtcttggcgg
    23761 acaggataaa ggcaacttaa ttgggataaa acccgatatt ctttgttccg attataccat
    23821 attaaaaaat cggagggaac cattatgaaa cgaaacttaa ttataggaag cattttaatc
    23881 ggcggaattt tcggcttatt accattaatt gcaaaaatag ctatctttgc aggattagcg
    23941 ttatatctct tcactgctta tgacgagtac gactatcaat taagaactgg agggcataag
    24001 tagatgtaca aaacaaaact attaaaccaa ctcgatagcc ttgaattaga ggaaattaat
    24061 caagggatcg ctgaactaga aaataatatc gggaaaacct actttggaaa ttcattcaat
    24121 gaaaaactaa cagttttgta tgtactcaaa aaacatgcag aacacaaaat aatttgtcga
    24181 gaaatcaacg agctaaaaaa tcagatttta accgcatggt taaacattac tgatatgcaa
    24241 gaagcaaggg tgaaaacatt taatacatgg gtaaagtatc agaatcaatt aaaaggtgct
    24301 gaatttgttc gggatgggtt gaaatacgaa cttgagcagt tgaagctcat ggaggtgtct
    24361 gaatgattta taccagtatt ttgaaaaagg aggagaggtt tattggcaaa taattatcaa
    24421 gcagcaacgg aattacaaaa gtcgggatgg tcaatttatc ccttatctcc tggaactaaa
    24481 attccaataa aaggctctag tggttggaat gatgcaacta acgattctaa tcaaattaat
    24541 gaatggtgga cggaagatgc aactagaaat attgggttaa tgcttgaaga tcatcaaatg
    24601 gtggtagttg acctagatca acattctgaa acacgaaacg gcgttgagaa ttataaaaaa
    24661 cttgcttcaa tgtatgatcc atttccttct acttatacgg aaatcacacc aggtaaagga
    24721 atccatttct tctttaaaat tcctgatggt gtaattatcc aacaagaaac aggtgcattt
    24781 gctgatatct tcgggcgtga tgataaaggt aaaagtttaa gcgggattga tattatcact
    24841 aaaggagttc caatagcgcc aactatgatt atttcaagaa gtgttgggag agcatatgaa
    24901 cctattgaaa acgtatctct aaataatatt gcaccagctc ccgattggct agtgaaccta
    24961 ctgctaaaaa aaccgcaaga aataaagact caataccgac ccgtagcaaa aacagggaca
    25021 gcaaaaaaat tagatatgat tgttcaaggc gctggcgaag gtagtagaaa cgatcattta
    25081 actaagttat gcggttggtt actatggcac ggtgtagata atcaaacttt agcagaattg
    25141 atttatacag caaatgcgta caatgaacca ccgctaaaag ataaagaagt gaatcaaatt
    25201 attcgatcga tgattaaaaa agatttgagg gggaaagctc atggctcata agcataaatt
    25261 agatagtatt ttagactttc ccgaagccag cgagcgtgaa gacaatatca ttgaattgaa
    25321 aacctggatg tcacgattaa gatgcaataa agacgatcag attaaaagta acagtgttgt
    25381 gaatgcagaa ttgattttaa ccaatgatag caatttagct ggaacaattg cttataacga
    25441 atttagcgga tacattcacc tattaaaaga ttctccttgg atcaatcgta ccgctggaga
    25501 atgggaagac agttttgagg atgccttaac ggcatatatt gaagaaaact acaatgtagt
    25561 ttttgatgac aacaaaatac acaaagcagt agtaaatgtg gctagaaaga atgttttcaa
    25621 cccagtaaaa gaacgtatcg aaaaagttaa atgggatcaa cagcctagac tggaaacact
    25681 gtttattgac cttttagggg tagacgacaa cctttacaca cgagaagtaa caaaacgttg
    25741 gatagtcgga tcagtcgcac gtatctataa accaggaata aagtttgaga tcgttccagt
    25801 tttggatgga ccgcaaggga ttggtaagag taccgttcca gcattacttt acacggatga
    25861 ttttttcacc gattcattag attcgctagg tgaaaagaaa gatgattata tgcagcttca
    25921 aggcaatgta attattgaac ttggagaatt atcttcaatg aacaaaacta aaattgaaca
    25981 gataaaaaac ttcatcagtg ccaagatcga taaaattaga cctccttatg gtcggaatgt
    26041 catagcttgg gcaaggcaat gtgtatttat tggaacgtcg aatgatgggc agtatttgaa
    26101 agatgataca ggaaaccgcc gtttttatcc tttgccttgt aagaataagc caaagatgaa
    26161 tccgtttaaa acaaaggatg aatattttct tcaagtgtta gctgaagcaa aggtgctttt
    26221 tgataaagga caacgaatct attttagtcc cgatgaagat aaagaagtct tagaaattgc
    26281 aaaagactat caagaggatg ccaagatgga gaatccaatt aaagaagcaa tcactaaata
    26341 tttggaaatg gaaatcccgt ataaatggga agaagctcca gcgtgggcga gacgttctta
    26401 ttatcagaac tatcctgaca gtaaatgtaa tgagaaaaat ttacagcatt tctataatgg
    26461 agctactatg caacaagagt tttatttagt tgatagcgta ctcaccgctg atattttgga
    26521 agctgttttt gataaacaag caaaggattt attgaatggt cgatcagatg cagaaactaa
    26581 aaagattgcc ttgattatta gcagtattcc tggttgggaa cggaagcaac tgccaaaaag
    26641 aaataaacga cgaggatttt acaataagtc aaatgcctat gaaaataaaa atcgagctgg
    26701 aaagtacaaa aagtaacggg tagtaacggg tcagtaacac ctagcccgtt actagtttac
    26761 ccttactgcc ccaagggata cagcaatttg gtaacggggt aacaccttat ttccttaaaa
    26821 cttttgaaat agttagctaa tatgctgggg aataatgtta ctggatttta cccgttacct
    26881 gttaccaaaa cgctagaacc cttgtccccg ttgggattga gtggtaacgg gtacctgtta
    26941 cgtaggtgtt accacgtgtt acgaatcaag gaggaaagta aatgctcaat attatttcaa
    27001 caaacaaagc accgaacttt caatatacag atgaaatgga tcggtttcta atgaacacgc
    27061 tggcatttag tgtgggattg gtaacagaag attattccac atttgatcca gaagtattaa
    27121 agataatgga agaagaacct gattggctgc aggagtcagt agcgtggtgt caatcgctag
    27181 tagtgggatc attggtagat agtggcaact atgatgatac aggtgagcta atggatgaat
    27241 ttaattgtct gcttaaccta tatgatcgag cgagacaacg agagcttaca tcgaatgagg
    27301 ataatttgtt tttaaacatc catgataagt tcttggcttt gctactaaca gatgatgaat
    27361 tgataactaa tttattggag gtagaataat tatgcatatg gaaagtatta gatacttcga
    27421 tggtgagaaa cacattgagt attcaaacac tcaagaagat gtagacttta ttagttttga
    27481 ggaagataaa atcactaaag ttaattttaa agacggaacg tgcttgaaga tagtttcgcc
    27541 ttatattgaa tacaaatcaa aatgggaaca agatgattct gacgaacgag atcgctttgg
    27601 ttggtaatct atggcagtta aaaaacaatg taatcatgct ggatgtaaaa cattgattga
    27661 ttataggcag aagtattgtg ggaagcataa agcaaagcaa acggcaatta agcgtgaaga
    27721 aagaaagcaa agcgaaggta aatattttca attctatcaa agtagaacat ggcgaaaggc
    27781 ttcatacttg tatcggttaa atcatccagt atgtgaggat tgcctagaag aaggcttgat
    27841 aaggaaagct gatgttgtag atcacaaaat tgaactaaaa gatgattgga atagacggtt
    27901 agatgaaact aattttcgtt ctttatgcca tgcacaccat aattccaaga cagccaatga
    27961 aagacaaaga cgagaaaaga gtaccctttg agttaagggg gcatagtgtg aaacttactg
    28021 acaatcgatg cctactcatt ttggtacaaa taaccgttgt gaaaaggcat aagggtaatt
    28081 acaaataacg attacacttg taatcacaag tgaaaaagta tataatgaaa gtaggagatt
    28141 tattgaaaaa tacaattgtt attactgata aaacagggaa acagcgcacc attgagttac
    28201 ctcattttgg tcaagtgacc attcaaatgc agaacggaaa aatcatttat atagataaat
    28261 tagataaaga aaaattctga tcgaaaaacg aaggaatgtg agcttaattg cttacgttcc
    28321 tttttctttt gtctgaaagg aggacaacat gggacaccct aaaatattac acgatacaaa
    28381 aggcaatata tcaagtgaag aaaaagcaat ccgtgtggac gctagagaag aattgttcaa
    28441 gcagcaacca ttaataaata tcacgccccc tgactggatg gcagcgagcg ctagaagtga
    28501 atggaatcgt atcgtaccaa cattaaaaaa agattatcca ttgagtgaag cagattatgg
    28561 ttcattggta gcctattgtt tagcctttgc tcgaatgaaa acagccgaag ccgagataag
    28621 aaaatcaggt acgtttatca catgcgaaaa cggagtaaag aaagccaatc cagcggttcg
    28681 ggttcaatca caagctatga gtgatttgaa aaaacaagcc acctcactag gtatgacctt
    28741 agaatcacga tcaaaactag ctttgaacaa ggttaaaaat gatgaacccg aagacccgtt
    28801 caaagagttg atgaattcat gaatgattac attgagaaag tcctatcagg taagctgatt
    28861 gcacccaaga aaattatcca agcgtgtgag cgtcatataa gcgatttgga gcgttctaag
    28921 tcagatagct tcccttatgt gttcgatgaa gaacaagcca ccaaagcgat taaattcatt
    28981 gagttgctac catctacgga cggtaaagca atcaagatgt taggatttca aaaattcatt
    29041 ctaggcagtc tttatggctg gcgtactaaa gaagggaatt acaggcgatt caatcgagcg
    29101 tttaccagta tgagccgtaa gaatgggaaa acgtatatcg caagtggcat ggctgccaat
    29161 gcgttgatta tggaacaaga accagcagaa gcaaggcaag tattgtttgt aagtaacgct
    29221 ttgaaacaag ctaaattggg ctatgatatg ctgtctaatt cacttagaaa cgtggtcaag
    29281 tctagtaagt ttttaagacc acaactgaaa attatgaact ctaagattca gcacttgccc
    29341 tctaattcgt tcgctatggc actggctagt gaaaccagca cgctagatgg gtttgcacca
    29401 acaaccgcaa ttcttgacga gtggcacgaa gcaaaaactc gtaaaacgta caacgtcatt
    29461 aagtcaggaa tgacccaaca aaagaacggc ttattgtgtg ttattagtac cgctgggctt
    29521 gatttaaacg ttcctatgta cgaggaatac ttattgttag atcgtgtgct aaaaggcgaa
    29581 gaacaagccg acaggtactt tatagcgata tgggaattgg acgatcccga agaaattcac
    29641 gatcaagaga aatggatcaa agccaatccg atttttgaaa gtgaagaaat caaaaaagta
    29701 atgattccaa ccattcaaga tgatgtgaac cttgctttga aacaaaataa ccttaattct
    29761 gtattggtga aaaacttcaa tttatggaga caagcgagtg aggacagcta tatgattgct
    29821 gaagactggc aggcaaccga agtagaacca caagatatta caggcaagcc cgtttatatc
    29881 ggagtggatt tatctaaaac agatgattta actagcgttt catggatcgt accgctagat
    29941 aacggcgaac tttattgtga ttcacatagc tttgtagcca ccaaatatgg gcttcaagac
    30001 aaagaaaagc gcgacggttt gccttatcga gaactagaaa aagctggtga gtgttccatt
    30061 actcaattag aaagtggaat cgtggactat gaccaagtat ttcagtttat tcaagatttg
    30121 attcaagaaa atgatttgga atgtatgggg atttgttatg acccgtataa cgctaattcg
    30181 cttatcagta aagctgaaaa agccaactac ccaatgttag aagtaagaca aggaacgatt
    30241 actctaaacg ttccgacccg aacttttaga gaacaagttt atgaaggcaa cgttattcac
    30301 aataaaaata cgattctcac ccatgcagtg aacaacgcta ttttaaaaac ggataacaac
    30361 ggcattcaga ttaataaatc aaagaacagt aacaaaattg atccaatagc tgcattaatc
    30421 aatgcctatg tgtttgcaat ggattacttc accacaacgg aaggagcgaa agcagacaat
    30481 gaattttata caagtgaaga attttctttc taattacatt cataccgttc ttttacttct
    30541 tggattggtg tgtgtgttgg ttgctatcac cttactaaca aatgtctact atggcttgtt
    30601 agcgctgggc atagtgctta ttggggtagc ggtcatgcta aatacagaac agaagggagg
    30661 ttaaaagatg gcatttttta aagcgagaca aaatacaacg ggagatcctt tcctacaaca
    30721 tgtggtatca atccaatcgg atgattacac caccagcttt acaagcgttc gtgcattaag
    30781 aaatagtgat gtgtttgcag ccgttcgaat tattgctagt gatattgctt caagtccgat
    30841 tcaattggtc aaaaacaata tgccacaagc tgataatgaa ctggtgaagt tactaaacga
    30901 gaaacctaat tcagaaatgg atggttggca tttaaagttt gctttggcgg tcaatatgtt
    30961 gctaaacggt aatagctttg cagagattaa acgcaacggt gaaaaggtag aagaaattca
    31021 cttattacct aactcaagtg taacggttac tcaattagat aatggtacgt tgtcttatca
    31081 gattggtgat aaaaaaagac gtgtgaagtc tagcgatatt ttgcacttca aatatttcac
    31141 ccaagatggt ttaacaggat taccaccact ttatgcgtta cgtgatgaac tgaaaattca
    31201 acaagctggc aaccgcacat tgcacaattt ctttactcgt ggtgtcagtg ggtcaggtat
    31261 tttgaaggtt cataagtcag atttagacgg atcagcaaag aacgcaattc gtgaaaagtt
    31321 tgaagaagcc aacggttcaa gtagtggaga taatgctctt agaacgatca ttcttgatga
    31381 aacaatggac tacaaaacat tagaaataaa caccgatgtt ttgaagctta tcaattccaa
    31441 tgattggaac acaaaacaaa ttgccaaggc gtttggtgta ccgattgagc gtttaggcgt
    31501 tgaaaatgaa cattctagca ccgttcaaag taatctccag tatatccaaa gtacgctgat
    31561 tcattacttt aatgtgtttg tgagtgaatt agatacgaaa cttgaaacca atatccgctt
    31621 taattccgat cagttactag agactgatcc tgaaacaaaa gtaaaaaatg tattggatca
    31681 ggtcaaaggg tcacttctca cgattaatga ggggcggtcg aaaatggggc taccccccat
    31741 ggatggtggc gatcgtttac tagcaagttt gaactttacg tatttagata cgttggagaa
    31801 atatcaatta aaagaacagg aaggagttac accagttgaa taatgaagaa gaaaaggaaa
    31861 aacggctgac agaagaagct gagctaacag ccgattctcc caaagtagag aaagaaaatg
    31921 aaaaacaacc aacagacggc aaaactattt caggctatgc gttgaaattc gggcaaccgt
    31981 caaaagattt aggcggcttt gtagaagtga ttacacccga agcattaaaa gaggtggatt
    32041 tatcaaatgt gttcttatta tatggacacg attattcaaa gccactagct agtgtcaaag
    32101 caggtacgtt gaaattgaat gtcgatgata caggattaca ttttgaagca gagctaaccg
    32161 acacgaccta ttcaaatgat gtgtatgaga atatctcaaa aggggttgtt gattctatgt
    32221 cctttgggtt cgtgcttgga ttagattcat tcgacaaaaa agaagatggc acaattgaac
    32281 gatcaataga taaaatcaaa gcacttaatg aaattagtgt cgtgaccgtt cccgcttatg
    32341 attcatcaaa tgtccaagta aataagcgtt cttacgaatc gtttatgagt aacaaccaag
    32401 caaaacaaac aaacaatagc ttagaatcca cttctaaagc acaaaaggag agtaataaca
    32461 tggaaaaaac tttaatcgat aacgaaaaaa cagaattgcg tgggtatgaa gaatatatcc
    32521 gttcacaagg cgaagtgcgt gatggagtca ctactgtaaa tgcagcggca gttgttcccg
    32581 aagaagtaat cggtgaagtc tttgatttga aacgttcaaa ttataactta gctcaatatg
    32641 caacagtaaa aacagtatca aatggacaag gtaaatatcc agtagcaact aaccaacaag
    32701 cagtgttagc aacaaaagct gaacttgctg aaattggtga tattgacgct gaaatgttta
    32761 cttcagttga ttataaagta gaaactcgtg ctggtaagat tgccttatca aatgaggttg
    32821 tggaagattc agcagtgaat attgtacaag aggtcaaaga tcagttagcg aaattggtag
    32881 aaaacactga caataagcat atcatggatt tattaaaaac attcactaag aaaacggctg
    32941 ctacgttgga cgatttgaaa caactataca atgtggcatt agacccagca ttaaataaaa
    33001 tggtaattct aaaccaaagc ggatattacc acctagatac attgaaagat tcagatggac
    33061 gttacatttt acaacccgat gtgacagcac ctagtggtaa atcattattc ggtatgccag
    33121 tagtattgat tgcagataca ttgtttgcca atcctaaagc tggaacattc cctatgatta
    33181 tgggggatat tgcacaatct atctttgttg ctcgtagaaa tcaagtaacg actcaatggg
    33241 aaaaattcga ttactactca caaggacttg cagtgatcgt tcgcaacgat tataagaaaa
    33301 ttgatcaaaa tgcttcagtg tatattgagt ttacgccagt tgtaacacca aaagtataga
    33361 aaaatattgg gcggcggttt atcccactgc ctttttttca ttaggagaga taaaatggta
    33421 attttggata gcataaaaaa aagtatgcgg atcgatcaca ctattgatga tgactttatt
    33481 caacaattga ttgatacagc aggcgaatat ataaaaagtg ctattgatag tagcgcaacg
    33541 gataaagata tggataatta tcagcaattt gatttggcgg tgtcattact tactcaacat
    33601 tggtatttga accgtcaaga agccagcagc gaacggatac cagtaacggt acaagcatta
    33661 gtacaacaaa tgagaggtgc ttattatgcc aatcattaag aatgtaaatg aattgactga
    33721 gagagtcaag tttaaaaaga caaaacgggt aaaagatgaa gatgggcaga tggtggatag
    33781 tgaagaaact gtatttgaat gttgggctaa tgtgcgttca caaatgctaa aagacgtgct
    33841 tgctagtgta ggtaccattc ttgaaggaac gttgacgttt attattcgat acgatcaaga
    33901 ttatgaacta acaaacgata tgaaagtagc ttggaaaaac aagagttata aaattatttc
    33961 aatcaatgaa ggaacggcgt ttaaagatta tacaacgatc atagctaaac agatttctta
    34021 gaatgattac acttgtaaac gttgtgggta tttagtataa ttaaggtagt aaatgaaggg
    34081 ttagctgcct gaattttgca agacttagct agtcgaaatc tattgttgga ctgaaaattg
    34141 taggtgtggt tgcaaacatg ctggactaag aaagatgata gatgatgaac gttcaatttt
    34201 aatacaggca tgctaggact gattggatgg ttttggcaag ctgattgtgt agaaatcaaa
    34261 gaacacgtct gttgtggacg tgtttttttt gttataattc aaaagaggtg atttaatgga
    34321 caaagcagag tttacaaatt tttattggaa gtattatcta catttagaaa atgagtttat
    34381 taataccaca gattttgtga tgctcgatga gaataatttc aaaacattct ctattgaata
    34441 tcaaaaacta ctactagcga ttggttcgga gtgtgagatt attttcaaag aactttgcgg
    34501 atttgatagc agtagcaata aaagcataac tgattttaaa gctgttatta aatctagcga
    34561 attaatgtgt ttggataatg gtgttagggt tatgaattct ttaacattga cttcactaaa
    34621 accttttgga gatgggtggc ctgaagatac accagattgg tggaaaatat ataacagagt
    34681 aaaacatggt cgttcttcaa attataagga agcaaatttg gaaaatgtgt tgtatgcttt
    34741 agcaagtcta tatttattag aggaatattt gcataagaaa gtaatactag aaggtgaagt
    34801 agactttata agtccagagt caaatctatt tacgttatct tgggaaagaa aacattcgtc
    34861 gatgcaaaaa gtgacgtttg aaaaagtgga ttcaaattat gtagctccac tcattgattt
    34921 taaagaacaa gaagaaattt aaaaaccccc acggcttatt gaagtttttg tatgccatat
    34981 tgtatgccat ttggtttaaa tcgcttgaaa aatagtgata gataactcta attcatttag
    35041 tcataaacgc tgttttgata acctttatag ttctttgata tgggtttctc attcatggcg
    35101 gaagaagaag aatagaacaa aagaatgaat taataagata taaaaaaagg gattgtgaca
    35161 aacgtcttgt cacaatccct ttttccaaat aaacgatgtt caatcagctg cccctcggca
    35221 ttcgctcgaa aaacggcgaa atatgaagaa ctattttgcc atttgtttct ctaataatca
    35281 ggtcaaaacg ttgacttcac agtctcttcc ttaaacggtg ttctcctgcc aactcctcgg
    35341 tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac
    35401 tcggagctga acggcaggct cacgacctct ttcttaaacg gtgttcaaaa gcttgcatct
    35461 gcggtaataa gttcaaccaa acgaaaatta tgaggagcta tttttgtttg gctgttctta
    35521 ttacttgatg caggacagct ttttcacaac ctcttttcgt ttagcactaa atttccttaa
    35581 aaagtagaaa agctttcgaa gcctcgtttg tttcttttac tgtataataa aagaaaagag
    35641 taacaatcgc taaaagatat aaacaagaga aaggaaatac aagatgattg gtctgattta
    35701 ttttgtggtg atcgttctgg cgaacacagt tggcgctatt tccgggatgg gtggaggcgt
    35761 gttgatcaaa ccgattcttg atcttatcgg cgcccattct gttgcaggga tttcttttta
    35821 ctcaacagtt gcggtattca cgatgtccat tgtttcgact gtgcgacaag tatcaagcgg
    35881 gaaatcactg aattggcaga ttgtcggttg ggtatctagt ggagctgttg ttggaggagt
    35941 agtcggcaac attgtattcg aagttttttt acagttattt gaaaacgaga agcatgtgca
    36001 gctgatccaa atttttttga cggtattgac cttggttttt gcgttcttct acaccaagca
    36061 tcatcagcca aaatttcatt tgacgtcatg gacttggtac cttatatgtg ggggggttct
    36121 gggcttttta gctagttttt taggtattgg tggtggacca gtcaatgttt ctttgttgat
    36181 gctgatgttt gcattgccaa taaaagaagc aacactatat tctctatcta cgattttctt
    36241 ttctcaactg gcaaaattgg taacgattgc tttaacaagt agctttatgc gctttgattt
    36301 atctatgttg ttttatgtta tccccgctgc tgttattgga ggtttgtggg gagcaagatt
    36361 tagcagggtt ttgtctccaa aaaaagtgac ctttattttt caagtaattg tcctcgtggt
    36421 tcttttgatc aatctttaca atgctttcgt catcttacaa acattaggag aatctagact
    36481 aacgcaagtc aaaatcactg aaacatattg ttctaaatga aaaaaggagt atactgtgat
    36541 aaaaaaattc atttaaaaaa ataaaaattg gctggtgatt tggatgttgc gggaaatgcg
    36601 tttaggagat gcagaagcac tacgagaaat caatgcggaa caattaggat atgatgtacc
    36661 attaaccgtt acggtgagac aaatgaagaa actgttgaag gagccagaaa aaaatttttt
    36721 cttggtctat gaagatccta cttctaaaaa agcagctggc tatgtacaag cggagtccta
    36781 tgaatcgatt ttcagcgatc cgatgttcaa tattatggca cttgctgtgt ctaaacatgc
    36841 tgaaaaaaga ggaatcggaa aggccttgat gactggcgta gaagaagcga aaagacgagg
    36901 aatatcagca ataagactta attctgcaga atacagagta gaagcacacc gattttatga
    36961 acgcatcggt tatcattccg ataaaatgca aaaaagattt ttgaaaatcc tgtaaatttt
    37021 tatcaaaaaa tacctggtca tcaagccgat acttgatgat caggtatttt ttttgactaa
    37081 gaaagtaatt gcatcaagaa acctgagatg atcaaaacga tggcaccacc aagccgattg
    37141 cccatttgag caaaagcaat aagttccatc cgattagctg cagataagac agctacattt
    37201 cctgtaccac ccatcgagtt gttgcataag ccagcagtaa tagttgattc gattggatat
    37261 aaaccaaaca aacgaccgac aaatcctgaa actactgaaa tcgcgataac acttgtcaaa
    37321 cataagacaa caaattgcca tgtcaaagca gaagccaata catttagatt caataacgca
    37381 ataccgatac cggcaagaac agctgctgtc aagtttttga caatcaagtt gttgaacatg
    37441 atagctgcat cttcgtaata ttctggtaat aggtttgcga ttttacagat aacaacgata
    37501 atgatcatga atgcgtaagc atggacttta ggaacaaaat agttacagat ttgtcctaag
    37561 atgaagaaag tcaatgatac catcaaaccg acacctaatt gtggaatgga aggttttaca
    37621 tttctaggtt ttccatcgcc aaggtcgcct ttttccatta atttaccatt accgttgaat
    37681 tttggcattg attgtcccgc acgagcaact aacgctgcac caatgatagc taggacattt
    37741 gtcattgctg tcgcaggaat caggcgggaa atgatagaag aagcgtccgt acctaaaaca
    37801 tttgcataaa tcgttgataa aggtacagct ccggctccaa caccaccagc cattgcgggc
    37861 aatgagatat ataaaacgga atttgcaaaa ccatttccga taagcatacc tacgagtcct
    37921 acggcaaaaa aggagacgac cattgaaata agtgcaacgg gaataaaacg aacagaagct
    37981 ttcatcaata gattacggtt cataccaagg atgctaccag taatcaaggc agcaatataa
    38041 aagtctaaaa agcccatatt gctgacaaag ttttctgtgg attttactac atactcaggt
    38101 aagatcccaa atgtagcaat cgcggcagag gcaaagatcg caaaaacgga gccaccacct
    38161 aaataacttt tgacgatagg gatttttgtt ccgataaaat gaaataaatt accgaaaatg
    38221 accaaaacag cgattgggcc aatcatattc gtcggtaatt ttcctaaagc cattgcaatg
    38281 atcaggacaa ggaccatgat caaataaagg ggcaaactta cgccggtaat ttttgttgac
    38341 caaaaccctt cttttttctt ttcctcaact gcagttgcgg ttgttgtttt ttgtgccatt
    38401 tttacagatc ctttcttatt ctgtatcgta tgaaaaaagc caaagagaag cggcaaaaga
    38461 gcagtagttg gctggctgct gttttgccgc agtcgctttt ttctttttag acgtcataca
    38521 aagtttctgt atgttctttt actataacaa tcttaatctt ctaaagattt atattcagga
    38581 acccatttag cagattcaac agcttctttg atatccgtga tttcttcacg attcaatttt
    38641 tgtgcaacaa ctgattttgc gacagtttca gcaatcgttt gagagaattc agtgattttt
    38701 gctactggag gcaagatagc tgctcctggt tttgttacat caacgattcc gcctaatgct
    38761 cgactagctt gagaaatgat ttcagcattt actcgtgtag ctgttgaagc aatcaaacca
    38821 agccctaaac ctgggtacat caacgcatta tttgcttgac cgatttggta agttacaccg
    38881 ttatattcca catcagcagc agggatacct gttccaacta aagcttttcc gtccgtccat
    38941 tcaatcaaat cttttgcttt tgcttctgcc aattttgttg gattagacaa aggcatgatg
    39001 attggacgtg gggtatgagc cgccatttct ttcacgattt cttcagtgaa tgctccaggt
    39061 tgggtagaag taccgatcat gatcgttgga tggattgctt ttacaactgc ttctaagttt
    39121 gtcaattcat cagcattagt gaattctgag cgtttacgag cgaatgggat ttgaccagga
    39181 gtcaatcctt ttgtatcttc aaacaataaa ccctgtttat ctacttgata gaaatgttgg
    39241 cgtgcttctt cttcaggtac accttgacgg atcatttcat ctaaaaggat gtttgcgata
    39301 cctacaccag cagtaccagc accaaatgtc aagatcgttt gatctttcaa ttgctcacct
    39361 gatatattca atccacctaa tacaccagct agtacaacga ttcctgtgcc ttggatatca
    39421 tcattgaaag tagtgatttt atcttcgtat ttttctaaga tcgttgcagc atttcctcgt
    39481 ccaaaatctt cccaatgtaa taacaattct gggaataatt cagtagctga ttgaacgaat
    39541 tgatcaatga attcgtagta agtttcgcct tctacacgag catgacgatt ccctaagtat
    39601 aatggattat tcaataattc ttcgttatta gtaccagcgt cgattgatac aggcaagact
    39661 tgtgcaggat tgataccagc cgcagcagtg tagaccatca atttaccgat agcgatgtca
    39721 acaccgttca caccccagtc acccatacct aagattcctt ctgcatctgt cacaacgatc
    39781 aaacgaatgt cgcgtccatc tgctgcgttt ttcaaacttg ctttgatatt ttctggttcg
    39841 tcgattgaaa gaaatgcagc atcttgtggt ttcaagaaaa tttcgttgta ttgttcgatt
    39901 gaatccgcaa cgacaggatc ataaacgaca ggcatgaatt caactaaatg ttcgcccatc
    39961 aatttataaa acaatgtccg gttcgtattg aatagattca ttaagaagat acgtttttcc
    40021 aaatcggtct gtttgcttaa gtattgaccg taagcttgga cagattgttg ttccaatgtt
    40081 tggacagtac ttggaagcat accagtgatt ccgtattttg cccgttcttc ttttgtaaaa
    40141 gctgttcctt tatttagaaa aggattattt aataagttta gccctgtaag catgaatgat
    40201 tcctcctaag ttttaataca aacgaagtat aggagtcaga tgaggatata gtcaaaaaga
    40261 gcaggtataa taaattttat ttatatgagt aatagagtca aaagaaatca aaatagcggt
    40321 atttgttctt tttatatcaa caatccttgc ttgattacta tgcgtatctc ttatatgcta
    40381 aagaaagaaa agtagaagaa taaaggatat gttagataga taaatagaat ctctgaagaa
    40441 taaatcacgt gatcgtgacg ataaaataca cttacgtcag gtgataaaaa atacataagt
    40501 agaaaactaa gaggaggaac agaaaatgaa agaagttcaa ttgatcagaa aaagcgaact
    40561 atcagaaggt ggttgcaatg catgcggcgt agtagaagca acaagctata cattaaagct
    40621 tggtgccaac aaagcgataa tctctgagtt gacagtaggc ggtcttgtgg acagtctggc
    40681 gcttgctgaa ggttttattg gagaagatat ttatgagatg ttttcagaaa tccgtcagtt
    40741 aaaaaaaggg gaaaactgca tagaggttca tcatgagagc ccgaatgtga gattcaaacg
    40801 tggtgataat gagatgatct tcaataatca tgtgtccgat cacaccgagc tatatggaat
    40861 cgtgaatcaa atactaactg agctttttgg attgggacct tatgcgttca aggaagaaaa
    40921 cggtaatcca aaattaaacg aagaatggca agaaacgatt gaaatacaaa gaaacaaccc
    40981 gcacttattt caataggcat ccacagtatt tcgtaaaagg gactttgaca cgacttttgt
    41041 caaagtccca tcgtttttta tctatttgtg ttctaaacat ttctttcaga gtagtaacaa
    41101 ctggaatgac tgaaatgtaa acagattatt tgcctgtatc aatacggaag agtaatataa
    41161 agtgtgtaaa cgctatcata ttattcattc aattttttat agagggaagg cattgaaatg
    41221 gaaaaaacgt atcaagcagg tacaaatgaa ggaatagtgg attttatcaa tatggaagat
    41281 ttagaaatag ccgcttcaca agtgattcca gctggtggtt atggttacat cagcagtggt
    41341 gctggcgact tatttactta tcaagaaaat gaacgggcat tcaatcatca gctgattatt
    41401 cctcatgtat taaaagatgt agaattaccc gatacaacta cacattttga caaagaaacg
    41461 ctgactgcac caatcatcat ggcaccagtt gctgctcatg gcttagctca tgtgaaggcg
    41521 gagaaagctt ctgcaaaagg tgtagcagat tttgggacga tctatacagc aagttcttac
    41581 gcttcttgta cattagaaga gataagagaa gcaggcgggg agaaagcacc gcaatggttt
    41641 caattttata tgagcaaaga tgatgggatc aatttggata ttcttgaagt ggccaagcgt
    41701 aatggagcaa aagcaatcgt attgacagcg gatgcaacag ttggcggaaa ccgtgaaaca
    41761 gaccgcagaa atgggtttac cttcccactg ccaatgccga tcgttcaggc ttatcaatca
    41821 ggagtaggcc aaacgatgga tgctgtgtac aaatcttcca aacagaagct tagtccaaaa
    41881 gatgtagaat ttattgctgc tcattctgat ttgccagtct atgtgaaagg tgtccagtca
    41941 gaagaagatg tctatcgttc attagaatct ggtgctggcg gaatctgggt atctaatcat
    42001 ggtggccgtc aactagatgg tggtccagca gcgtttgatt cgttgcaata tgtggcagaa
    42061 gcagtagata aacgtgtacc aatcgtgttt gacagcggtg tacgacgcgg acagcatgtt
    42121 ttcaaagcaa tcgcatcagg tgcagatctt gttgctattg gcagacccgt tatttatgga
    42181 ttgtctttag gaggaagtac aggagtccat caagtgtttg atttctttaa aacagaattg
    42241 gaaatggtca tgcaattagc tggaacacaa acagtagaag atatcaaaaa aataaaattg
    42301 cgtgagaatc gttttattta agaaacaaat gacgaaaaaa ataggagtgc ggcagatgat
    42361 gccgtactcc tatttttata gttcaaggat gtcactaata gaataatttg aaacgaaaaa
    42421 ataaaattga aatatttttt catacttaat aaaaaaggaa gaatattcat gtaaacgatt
    42481 ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc ggatgaattc
    42541 atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa aggagtgatt
    42601 tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc taggcggctt
    42661 ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta cctttactga
    42721 accaacaaat accgtggagc ctcttaaccc tactgatcct tcaaaaccag tagagccagc
    42781 agatccggaa aatccagcaa ctggccagac aggtccttta acactggatg tagttcccga
    42841 attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag tagacgcttc
    42901 aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg cagatggcca
    42961 agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt cacaagtttt
    43021 gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag ataacgaatc
    43081 cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag cgacaccaat
    43141 atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg atccagcaaa
    43201 tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt tgacttggaa
    43261 cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg cggtgaaaat
    43321 actataggtg aaggattttt taccgcagtt tttgttgaaa ggatgtggtg gacaatgaat
    43381 caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt catttttccg
    43441 tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt atttccagag
    43501 aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga acaaaatcag
    43561 acactagaag taattatcag caattcttca gatgaagaac gaacatttga ggtctctgtc
    43621 aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa cccaacgtta
    43681 gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa ggaaatcaat
    43741 gtatcagcac atgcagaaac aactgtacct atagaagtga aaatcccagc aaaaagcttt
    43801 aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga aacagaaaat
    43861 gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt cgttttacaa
    43921 gaaagtcaag agacgataga accggatctg aaacttttat caggagattt ggacgaggtc
    43981 aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat tatcagcaat
    44041 ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa tacgtctaat
    44101 gcatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc tggtgataaa
    44161 gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag taatgaatgg
    44221 cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa tgaaaattcc
    44281 gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt tgctgcagtt
    44341 ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa aaaaggagaa
    44401 acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga ttattgcaag
    44461 tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt caggtacgat
    44521 tacattttat gatctaagtc caattccgca tccaagtggt tcagtcaatg gcgcatcaaa
    44581 acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat tcccggagac
    44641 gaatgaacaa aagatccgtt ttctttattt gatcattggt atagaattat ggcttcttgc
    44701 gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta caaaataaat
    44761 tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata tttatctttt
    44821 caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat ccatacacgt
    44881 atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg aagcttgtcc
    44941 aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac gatggttcaa
    45001 atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg aaactaacaa
    45061 atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg atcatcaatt
    45121 tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac aacgcctact
    45181 tgtatattgc cggtgccccc atcaaggtta ctagcggaag tttattatca gcaggaagcc
    45241 ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattgaca gatattattg
    45301 ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt gatgttacag
    45361 ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca aacaaaattt
    45421 tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct gttgattttt
    45481 atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct ccagctgcag
    45541 gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat gcattttctg
    45601 caagtgcaga gataaatacg tggaatagtt acatctcacc aagtgattca acaggaaaat
    45661 ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac cttccaggct
    45721 ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat cagttgccat
    45781 ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca caaggagcaa
    45841 tactgactca tactccagca agcacaagtg gattgaatca gacagccaat atcgtgtaca
    45901 gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta ttggagcagt
    45961 acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag tggggaaagc
    46021 tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa ctaaaaaagg
    46081 gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca ggatggacag
    46141 atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct acttctacac
    46201 tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac gatgtactta
    46261 ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga atcaccactc
    46321 ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag ctaaaaaacc
    46381 ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat acgattcaag
    46441 aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa aaaatcacga
    46501 tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat ggtacaacta
    46561 tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg agcaaaatag
    46621 gagataccat tcatcttact tacaactatc aattaaatac tgtagatact tctttacaat
    46681 ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta gcagatggtg
    46741 ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc cccaaacaag
    46801 aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc cccaaaccgt
    46861 tgaagacaag ttattatgaa tcaaaaggcg atttttcttt tgatgtgcgg gatactcggc
    46921 ttccaagcac gtctccttgg caactgacag gtacattgac ttctttattt aagaatgatc
    46981 aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca aagcaattga
    47041 ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa ggagaggttc
    47101 tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt acgaatgctc
    47161 aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg ccaaccagct
    47221 aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt ttcaaggatg
    47281 agaaggaaac ttctgctgtt aaaatcagaa atgcttgaag gagtggactt ggaacttttc
    47341 agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt attagaggaa
    47401 aaaggagagg tttccgttaa taacgtactt acccgcttat cagttgatcg ggctaccttg
    47461 aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt ggaaattgtt
    47521 atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt cgatgtttat
    47581 tatcattgtt acttgcgcta ttctacaaaa tatcagatac ttctttatct gttggaaaat
    47641 ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc tactttgtct
    47701 cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat aaaaagcggg
    47761 cggctgaccg gtccagaaag tcaaattcgt tatttttatt tccaattatt ttggtttgga
    47821 cgtccctatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac ttttttagat
    47881 ctgctgcaaa aagagtttag ttttgctatt acagaagaag gacgattgaa atttgaaata
    47941 tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc agattatagc
    48001 aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca tacatttttg
    48061 acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt ggttttttat
    48121 cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca ggagtttttg
    48181 ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt taaacaatcg
    48241 atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa gattgcgttg
    48301 acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa tatttttggc
    48361 caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct ggttttatgt
    48421 gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga cgaggaaaat
    48481 gcccgctttg aattatttgc tcgctatgca tctattttac ggacgctgat caagcagtta
    48541 gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc ggatatcgtg
    48601 aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt cgaatacgag
    48661 aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc gtcacagaag
    48721 tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc ctatttgaac
    48781 caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa aacatgaaaa
    48841 tcgtgatcat tggtgcttcc catgaaggca tcacagctgc cctaaatcta agaaaacttc
    48901 aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta ggctatgttt
    48961 caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa gttgcccata
    49021 acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga gtaactgaac
    49081 ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac gagaccataa
    49141 cctatgataa attaatcgta gcgacaggtt cctctcctgt caccttgtat aaacaaattg
    49201 aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta cttgccgctt
    49261 taaaagaagc aaaagaagta gtgatcttcg gtgctggtta cattggattg gaattagcag
    49321 atgctttgag aaataaagga tacattattc atctagttga ttatatgccg aatgttttat
    49381 caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa actaagcaaa
    49441 taaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag aaagtagtat
    49501 ccgtacaact tctgtctcaa acaataaagg cagatatggt cattttttct gcccaaactc
    49561 gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag actgtgatag
    49621 tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat atcgtcccag
    49681 tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga gctgtacata
    49741 tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac ctcagacaaa
    49801 agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg acagaagatg
    49861 aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat ctatttccac
    49921 aatacgacga agaaaacaaa acagtaaatg caaagctgat ctaccatccg gatacattag
    49981 aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg aacctgcttg
    50041 cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat tttggatttc
    50101 tgtctgaata cacctctcgt tttcactacc tcaatgagtt agcttttaaa gtactagcta
    50161 agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg tcttttattg
    50221 tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa tgaaatagcg
    50281 gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa ttgttgtata
    50341 aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa ttatgcattc
    50401 aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc tggtattttt
    50461 tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg acaagtggga
    50521 ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt ccatgattac
    50581 taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc aaacacctgg
    50641 agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct tcttcggtat
    50701 cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg ataacgatga
    50761 tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccatag gagatacagt
    50821 taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc atgccaaatt
    50881 caatgtctca ccagttctct atacaaccat caatgcttac caagaaatcc gcttcgaaaa
    50941 agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta aaatcagtaa
    51001 tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat tacctggata
    51061 taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta ttgcggctat
    51121 tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct ttggtgtaat
    51181 gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac aaacattcat
    51241 tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat cccttgttct
    51301 acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta gcttgttgat
    51361 gttagtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa aaatcgatcc
    51421 gttgaaggca attggataag aaggaggaag aacatgaaag caatagaatt taaagaagta
    51481 gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac aaccatgagt
    51541 atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa aagtactttt
    51601 ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat caatgatcaa
    51661 gaattcagta atcaaaaaga aagcgaacgc gttaaactac gttttaaaga aattggcttt
    51721 attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt attattagta
    51781 gataagatta aacatgaaca tcgtcaaaaa gaagcaaacg aattatttga gcagcttggc
    51841 gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg gcaaagggtc
    51901 gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga accaactgct
    51961 agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga atcaaaagac
    52021 aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa atgcgataga
    52081 atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag caggtggaaa
    52141 aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag cgactgactt
    52201 gtttatgtcc aagggatatc tcggcacttc aacaagagaa atcgcacaaa aagttgggat
    52261 cacccagccc aatctctatc attatttcgg tgataaagaa aaactatata cggcagtttt
    52321 agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa caagcgaaac
    52381 gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac atctggtcga
    52441 tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa gagatcatct
    52501 tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt cagcaaacca
    52561 atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt tcttagtgtt
    52621 agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg tggcgcaatt
    52681 gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata tagggagaaa
    52741 atagttaggt gaagaagcaa gatatgacga agatcgttat atcttgcttc tttatatagg
    52801 tagtttttca taaatacaaa tttgttattc tctcatgatt atcagggtat atttttattt
    52861 attatgtcgg tgttccagga agtgttttaa tttaataacg aagaaattaa ttgtttgtag
    52921 aatgaaggaa actatttatt gggtagatga atcaccacga atagtcaaaa ggagatttca
    52981 ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg ttattttcta cggttatatc
    53041 ttttacagcc ccttttttgc ttacttaagc tgattttaag ttttggccct tagtctggat
    53101 ctacgaggtg agagtcaaga atgaaaaaaa taattggagc ttttgtattt gcaatcatta
    53161 tcattattat ctgttttcaa atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg
    53221 tcagcagtac gaacagtacg gttacaaaga caaaggaatc atttgatgat acagaacaag
    53281 agacattaat caaaatcgga aaaaatgacc gtacactata tacagatgac tcgctaacta
    53341 ccccacttgc aactataaac ggcggagagc tggcggagtt tctttctgaa acaaaaaatg
    53401 cttatcagat caagacaaat gatggctata ctggatattt agccctcgaa gatggaacca
    53461 aagtcacaaa gaatatacaa acaaaaccgg aagagctatc tgatgcagtg attgtattag
    53521 atcctgggca tggcggaaat gatactgggg cattaagcaa tgatgaacaa acagaagaaa
    53581 aagatataac attaagtaca gccataaaag taaaaaaggc gctagaggat gctggagcaa
    53641 cggtttattt gacccatact acagatgaat tagtacagct tggagatata tgcgactaca
    53701 gtgaagaaaa aaaggcagat gtctttatca gtttacacgc agactctacg gaatatgcga
    53761 atgaagcaac tggaatcacg acatattatt actatggaca agaggaaaca ttagcacaga
    53821 caattgctga ctccttcact gatctgcctc tggattcaag aggaatctcc actgggaact
    53881 atcaagttct tcgagaaaat cttcaacctt ctattttaat cgagatgggt tatatgaata
    53941 atgatagtga tttaaaggaa ttagtaacag acagttatca gcagcagatt gctgcaagtt
    54001 tgacacaagc attgaccact tattttcagt aataaataag cccagccaaa caaaaatagc
    54061 tcctcatatt tttcgtttgg ccgggcttat tatcgcagat gcaagctttt aaacaccgtt
    54121 taaggaagag gtcgtgagcc tgccgttcaa ctctgagtca caaggagcaa tttcaaaaaa
    54181 cagcttctca tgtttgatga ctattgtgac ttgtgaccga ggagttggca tgagaacacc
    54241 gtttaagaaa aaggttgtga aatcaacgtt ttgttctgag tattagagaa acaaacggaa
    54301 aaatagttcc tcatattttg ccgtttgttg agctaatacc gaggatcaga tgattgaaca
    54361 ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat tattttttta
    54421 gtagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt tcaagcatag
    54481 gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt ccagctcctg
    54541 acggaagggt gattccagct tcagctgatg ctttgtctaa ctcttccaaa aagctggcac
    54601 gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta gtaacgaagt
    54661 acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa tttgcttccg
    54721 gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt tttagcagca
    54781 aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat tttggttgga
    54841 tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt ttttttaaga
    54901 tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta acaccaagtt
    54961 tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt agcggaccga
    55021 aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg atattttttg
    55081 gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc ccccatttat
    55141 cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta taagcggtaa
    55201 tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta tttaacaaat
    55261 aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg ataatgacgt
    55321 tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca cgacttttcg
    55381 ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa tagttaagta
    55441 tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac taaataaaac
    55501 tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct acaaagcggt
    55561 gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata tggatatcgt
    55621 aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac aaatggataa
    55681 tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga agaaacaaga
    55741 aagaatattg gagctggaag aagaaacagc agaattaaag aaaaaaatga tcaaatttac
    55801 agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc gggaagtatt
    55861 gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac aacagatttt
    55921 aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc cttttatcaa
    55981 aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga gtttgctgat
    56041 tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg ctttgcaaat
    56101 catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt acaaaccatt
    56161 agcaagtcat ttggaattat acattccata cccagctgtc accccaacat caaagttagc
    56221 attttttgat caggtatttg cctttcacct agatgaagct ttttatgcag gaactgcttt
    56281 tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt ttgtccatgg
    56341 attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca tcctgtcact
    56401 aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg ttccattaga
    56461 tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg agagaacgcc
    56521 atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt aaggaaaata
    56581 gtgctggaaa atatttcgtt ttttgtacag agtagtttat ttaagaaggc tgcgtagcgt
    56641 atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga ataaccgaat
    56701 ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg tagtgactgc
    56761 acaaggaaaa gaagaactgg cagaattggt accagtgagt gagaaacaaa cgatcacaaa
    56821 ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg ggatccctat
    56881 acctaaaatt gaaaatatcc gtccccatat gaaacggatt gagatcggag cagacttgaa
    56941 tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat tgaatcgttt
    57001 cattgatgat ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt gggcagatca
    57061 gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg aagacggacg
    57121 cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc ggcgaagtga
    57181 acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa agtatttaag
    57241 tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac aagaataccg
    57301 cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggtcagactt tatttattga
    57361 accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg ctgaacgaaa
    57421 tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc gtcaagaaat
    57481 catccacaat acttatgtga ttggtaaaat ggatctgatg aatgcaaaag cacgatttgg
    57541 taaagaagta aaagccatcg tgccgggaat cagtgaagac aatcatgtcg tcttgaaaca
    57601 agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca caatcggaaa
    57661 agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga tcacgttgaa
    57721 gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg caggtgaaga
    57781 aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac agtcgattga
    57841 gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt tatcaaaagt
    57901 caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc cgcaagaagg
    57961 tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt atgtgatggc
    58021 aacaacgcat tatcctgaat tgaaagttta tggatataat cgggcaaata cgattaatgc
    58081 cagcatggag tttgatgtgg atacattaag tccaacgtat cgtttgctga ttggcgttcc
    58141 tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag aagttattga
    58201 tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga tcacagattt
    58261 agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt ttgtttctga
    58321 ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg aagaacgtga
    58381 aaaagaaatg gaaaaagcaa agaaaaaagc aaatgaagtc gtgtcagagg cggaagaaaa
    58441 agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc aaggaaatgt
    58501 gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc aagaagaaac
    58561 tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga aaccgggaga
    58621 cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa acgggaatca
    58681 gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc tgacgcctgt
    58741 tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg aatctagtag
    58801 tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc taagtgaggt
    58861 agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta ttgtccacgg
    58921 aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc atcggagcgt
    58981 caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa ttgttaaatt
    59041 taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga aatgctatac
    59101 tagtcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac agataagaca
    59161 tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac ttggtgtggt
    59221 ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga tgaagaagaa
    59281 ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca attcggtatc
    59341 atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa agctgttggt
    59401 gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa acttacagca
    59461 aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg cggttgagtt
    59521 aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg attgtttttt
    59581 tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga tgaacgaacg
    59641 aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga tgaaagataa
    59701 aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg tccgctcata
    59761 ttttactggt agccataata cgaaaacgga acggcttgtt agtgaaatcg aagactttga
    59821 atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc tgataaagaa
    59881 aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt tccttaagat
    59941 aacgaatgaa aaatatcccc gtttggtgat tactcgtaag gtactaaaag acaaagcact
    60001 gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga tcctcgatcg
    60061 attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc tttactatca
    60121 tttgggacaa tgcctgtgtc cttattattt cgacgtagat ccgaaagtct atacgaaaat
    60181 cgttgatgaa gtgaaaaact ttttaaacgg aggacacgaa aaaatcgagt cagagatcca
    60241 tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag aataccgaga
    60301 ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata ctgatttact
    60361 ggatcgagat gtatttggtt atgcgattga taaaggatgg atgtgtgtcc aagtgttttt
    60421 cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct ataacgaagc
    60481 agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc atttcattcc
    60541 taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt tgttagctac
    60601 gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg cgagtaagaa
    60661 tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg aacggacgat
    60721 cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac gtatcgagtc
    60781 ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg tattcattga
    60841 tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca acggaccgga
    60901 tgattatgca tcaatgagag aagtcatcta tcgcagatat tcgcgagtga ttcgcgaagg
    60961 gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag atgtggcaaa
    61021 agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg taaaaaacga
    61081 taaacataaa acaaatgagt tgctattcgg tacagatttg gctgtagtac cgcttgagcg
    61141 aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc gattcgcaat
    61201 cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag atgaaattga
    61261 agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt tgaaaaatat
    61321 cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg tcgcacaaaa
    61381 cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa agtaaagtgt
    61441 cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat aataaatcgc
    61501 ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag aaatgttaga
    61561 agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg tctctttcca
    61621 aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga agacaacgac
    61681 ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat ggaatggcca
    61741 tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac ggggacttta
    61801 tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag ggaaatcaag
    61861 aaaagagatt gaaccaaaaa tcgacttttg gatggaaaaa tttcaggtaa aagggaaaaa
    61921 gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc tgatcgccac
    61981 gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt tagatccggt
    62041 aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt catgtgtcat
    62101 tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga tcatgttgcg
    62161 aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat ttggccgtac
    62221 gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg aaggcgtgaa
    62281 aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc ctgaagctgg
    62341 gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta accagcaacc
    62401 gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt aagttttgga
    62461 ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg atcatgatat
    62521 tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct caacaaaata
    62581 gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag caaatgagcc
    62641 aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa gcacaaaaag
    62701 agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat gataagataa
    62761 atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg atacaacaac
    62821 aactgactgg cttgcaatcg atgcttcgag cgagtcaatt aggcatttca ccagaagaag
    62881 tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt gattcaaaag
    62941 gaaaaatgac agtcggagaa gataactcga cagtccaata cgtcgtaagt tatgtagcaa
    63001 cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa gagatcgctt
    63061 ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca caaaagcatt
    63121 tttacggaaa attaacaggt gttttacttg ttgcattaac gcaaatggca ttatacggta
    63181 ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa aatatattag
    63241 acggtatctc attggataac ttgattggac cattcttgtg gtattctctt ctattcatga
    63301 tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg gtcaacaaag
    63361 cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc ggatacatgc
    63421 ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc acttcgtata
    63481 ttccattctt atcttcatat attatgccga ttcgcttagc aaatgagaca gtcggtatgg
    63541 gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg atgatcttgt
    63601 ctgcaaacat gtacaaatcg aatgtactcg tttatagcga aggcggatta tggagttcat
    63661 tgaaacaatc gatttcaatt atgaggaatg aacgaaaaaa agcataaaac aatcctaaaa
    63721 tacagaagga agctggacat ctattgttcc cagcttcttt ttgtatagac ctattttatt
    63781 tattttacta tattggatat atattcattg aaaatagtgt ataacaactt tataatattg
    63841 agtataccaa tttttggagg cgttttaatg gaccgttatt tagatttact ttcagaaaaa
    63901 ttcccctcaa cggaggatgt catcacggaa atcatcaatc tcgaagcaat tttgcagctt
    63961 ccaaaaggaa cagagttatt tttgagtgat atacatggcg aatttcctgc ttttgatcat
    64021 attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt ttctgaacaa
    64081 atgacggaaa cggatatcag ccaattcact atttttacgg cttatccaga atatgcatta
    64141 acgactgatt ggtacaaaga acaagataaa tcgcagctga tccatcattt gatcgatctt
    64201 ttacggttta cgacagtcaa atatacccgg tcaaaagttc gaaaaggatt gccgaaagaa
    64261 tatagctata tcattgagga actcttgtat atcgatgacc gtatcaatgg aaaaaaagac
    64321 tatgtaaaaa aaatcattga acaacttgtt ttgatgaagg aagaagaaaa atttttaacg
    64381 aaacttgcac agacgatcca aaaatctgtc atcgatcatt tgcatatcgt tggagatatt
    64441 tttgaccgag gaactcaagc agccaaagtg atggatcgaa tcatggattt ttcttctgta
    64501 gatatccaat ggggaaatca tgatattttg tggattggtg cgtattgcgg ctctgaggca
    64561 tgtttactga cacttttacg gattgcagca cgctataatt atttatttga attagagaaa
    64621 gaatatgggt tgaatttacg tccattgttt tcttttgctc atcaaactta tcagaagaat
    64681 ccagtatttg cgcctaaaaa cagtaaaaat ctatccgaaa gagagcaaga agaattagaa
    64741 aaaatccatc aggcactttc tatccttcaa ttcaaatcag aacatcagtt attggatcgt
    64801 cgacctgaat ttaaaatgga cgatcgtaaa ttgctggaaa aaatcgacta tgaagcatca
    64861 acgattgatt tagaaggtca gctctatgag ttgaaaggaa cttgttttca aacgatccag
    64921 ccagatgatc caaaaaaatt cttggaagag gaacaaaaag tgattgatag tttgatgtat
    64981 tcattccagc actctttgag aatgcaaaaa catatgacgt ttttgatcga aaaaggggga
    65041 atgtatctca cgaacaatca gcatgtgctg tttcatggct gtatacctgt agacgagaag
    65101 ggacagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact gctgggcttt
    65161 tttgaaaaac agataactga aagcgcaaaa gatctgactg caaaagaaga tttagcaacc
    65221 gatttgattt ggtatgcgtg gagtgggcca ttttcgcctt tattcggcaa gagtaaaatg
    65281 gcgacttttg aacgttattt tctcacagag cctcatacgc acgtcgagaa aagcaatcct
    65341 tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttagcaga attcgacaca
    65401 aaagaagaag ggtcagcaat cattaatggg cacacgccag taaaagtaaa aaaaggggag
    65461 tcaccgatca aagcaaatgg aaaagtattt gtcattgacg gaggtttatc caaagcttat
    65521 caaaaaacga caggaattgc cggttattca ctattatgca attcttatgg tttccaaatc
    65581 gtgacacatt atccgttttt gcctattgaa caacaatttg caaaaaaatc cgaccaaaca
    65641 aatgtgaaaa aagtaatcga acagcaacta ccacgcaaac tcaatcgtga tacaacaaaa
    65701 ggtatggaac tgcagcagca gattacccag ctcaaaaggc tgttggacta tcgaaaggac
    65761 gattgagaag aattccagtg aacattgttc catagactgg agactgagac aaaagtcgtg
    65821 tcgcagtctc tttttccgga taaacggtgt tcttctgcca actcctcggt cacaagtcac
    65881 actattcaac aaacatgaaa agctgttttt tgaaattgct ccttgtgact cagagttgaa
    65941 cggcaggctc acgacctctt ggtataaacg gtgttcaaaa gccagtttct cggtattaag
    66001 ccaaatttat gcaaaaatgt gaaaagccat tttcacaaaa tttttcttaa tactcgaaac
    66061 taaacagctt tttcacaacc ccctcagaaa gtctattaaa attcattcac aaagaaatcg
    66121 aataagcgct gttcagatgg atgtacacgg tgggtaagtt ccggatgcca ttgaacaccg
    66181 aggattttat gcatatcatc tttgctttga accgcttcaa cgagtccatc gttagaaaaa
    66241 gcgatggctt ttaataaagg tgaaagatct ttgactgcct gatgatggta agaattcaca
    66301 aaatatggat cggggagaag gttgcttaac aaactgtctg aaacaatctt gacttcgtga
    66361 gtcgtaaatt gcggggcagt gggctgttgt tcatgtttta ctgaccactt tggatataat
    66421 gaaagatctt gataaagtgt accttccaaa acgacattca atagttggag tccccggcag
    66481 actgcaaaaa ttggttttct ttgtttcaat gcttccaaga tcagtgcctg ttcaaattgg
    66541 tctcgttgaa tatttgtttc cgttagcttt ggatgaggat cttccatgta aagttgtgga
    66601 ctgacatctt gaccacctgc aagaataagt ttgtcgattt gtccaatcag ttgaggagct
    66661 gaatctggag acatgactgg cagaataaga ggcaatcctt gagcatctaa aactgcatcc
    66721 acgaatcctt gcggtgtata gctcacttga tttccttgga aagtatctgt tgctcgaatc
    66781 agttgatttc cggcaatgcc gatgattggt ttcatttgta ttccccctaa agtagactta
    66841 ttgtaacaag gatttaaaaa aaattaaact atgtaagact tgcccctata cttatctgtt
    66901 cttgctgtgt tactatctgt aaagagaaat ttcgtgtgaa attcatgacg atagttgaaa
    66961 atattgctta atttgttata ataaaaagta agcagaaata aaaaggttgg gcgtaagctt
    67021 caaggatcta gttcttcaag gggtgagaag gacattttat tgaaggggcc tgcctatact
    67081 aagttatctt tagaggtaca tactttcaat gaaaaaagaa agcatgtacc tcttttttaa
    67141 atcgaaagga agtgaagcaa gtgttagaag ctttggaaca aatccgaaaa gcagaagaag
    67201 aaaatgaaaa gcgtgctcaa acactgcggc agcagctgaa gatctatgca acagaaaaag
    67261 agcaggaatt ggaaacgatc caaacaaact atcgacaaca actaaaacag ttggtggaag
    67321 aaaaagaaca atttgaaata caagaattag aaaaagaaaa aatgaaattg aaagcagaag
    67381 cagacaaggt gcaagcaact ttgaaagatc aatatcaaaa gcatcagagt caagcgatcg
    67441 gtgcgatcat tgaaagggtg aaagaaactt atggccgtca ctaagatgga aaaagtgacc
    67501 ctcatttcag ataaaagaaa tcaggagagg attttacaag cagtacaagg aatccaaaac
    67561 gttgaaatcc gggatctgta tcaaaataga acaaacaacc agtgggtgga gaagtatttt
    67621 ccagatacca cgattattga taaagaagca aaattaagcg ctttaggtaa tcggttgaaa
    67681 gacatccaag aagctatcca attcatacaa caccacggcg atagccgtca aaagaaactc
    67741 catttgaaac gtcaagaact ttccttacaa gatttggaag ccaattattc cgaagaaaat
    67801 ttcataaata aattgaaaga agtccttgat ttaaaagaaa agtgggaaca actaactgaa
    67861 gaacgtgatc gtttagaaga agaggaagaa tggcttcttc attggcaaca gctggatatt
    67921 gctccaagta tgtatgcaag taagacgacc atttttgaga tggggactgt gagtgcagca
    67981 aattttgaaa gtttcaagga agaactgagt aagcttgatg acgtttatat ggaagaaata
    68041 gattatacgc caaaacaagt ttatctttcg tatgttgctt taaaaaaatc agttgctgtt
    68101 atagaagaaa ttgctgggaa atacgggttc aataaagaaa cgtatcctta tgaacaaaat
    68161 cctaaagaaa aactgcaaga gaccaagcag caattatcca ttgttcacga gcagcaaaag
    68221 aagataacga cagcgttagg tcagtgcagt ggctatatcc aagaatttga atgggtagaa
    68281 gaagtcacac ttgccgttgc agaacgagaa aaaataaaag aacggtttat ccatgcgact
    68341 tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa aacagaattt gctgcatgtg
    68401 ttaaacgaaa ccgtatcaga agaagatata tacgtcagtt tcgaaacacc aaactcagaa
    68461 gagattcagc aggaagtccc tactaaattg aaaaaccatc cgcttgtcga accttttgaa
    68521 ttgctgactg aaatgtacag tctgccaaaa tatgaagaag tagatccaac accttggatg
    68581 acaccatttt atcttgtctt cttcgggatg atggtcgcag atgttggtta tggtttgctg
    68641 atgctcattg ggacaatact agctcaaaaa ctacttgttc taccacgcgg gatgaaacgt
    68701 ttcgtaaaat tctttgagat tctctcgata ccatcgatca tctggggatt gatctacagt
    68761 tcatttttcg gacagtcatt accaaaagaa ctgtttggca tccgcttgcc tttcccaatc
    68821 ctatctacga cggatgacgt caatacgatt ttgattttat ccgtgatttt cggtctgata
    68881 cagattttgg ttggattatt tgtggcagca aaggaaaata tcaggagaaa agattacttg
    68941 agtgctgtca gtgatggatt tgcctggcaa gggatcctaa ttgggatcgt tattgcgttg
    69001 gttggtgcca tgttgataaa aaatacagca tttgtttacc ttggcgctgg gctatcgatc
    69061 attgctgcct tatgtatctt gatcgttccg attatccaaa cgccgtctaa agtcaaaggt
    69121 gctgccaaag gtgcgtataa tctttacgga ctgaccggct atatcggtga tttagtcagt
    69181 tatacacgtt tgatggcatt gggtatttca ggcggaagta ttggagcggc attcaatatg
    69241 ctagttgctt tcatgccgcc agctgctcga ttcagtgtag ggatcctttt gatcatcgct
    69301 ttacacgcac tgaatatgtt tttgacactg ctaagtgcct atgtccatgg cgcacgattg
    69361 cagtatgtcg aattttttgg gaagttttat acaggaggcg gacgtgcctt tgatccgctg
    69421 aaaacggcgg aaaaatacgt caacatcaat cacaagaaaa aaaacaataa ataattggag
    69481 gatttttata taatgatgga ttacttaatt agtcaaaatg gtggaatggt atttgcggta
    69541 ttagcgatgg caacagcgac gattttttca gggatcggtt cagctaaagg tgttggaatg
    69601 acaggtgagg cagcagcagc attgacaaca agtcaacccg aaaaattcgg gcaggctctg
    69661 attctgcaat tgcttccagg cacgcaaggt ttgtatggat tcgttattgc ctttttgatt
    69721 tttatcaact taggaaatga catgtcagtt gtacaaggac tgaactttct aggtgcttct
    69781 ctgccgattg cttttacagg cttgttttca ggtattgccc aaggaaaagt cgcagcggca
    69841 ggtattcaaa ttttagcgaa aaaacctgaa catgctacaa aaggaatcat ttttgctgcg
    69901 atggttgaaa catacgcgat tttaggtttc gttatttcct tcctattagt gttgaatgcc
    69961 taagcataaa ggaggaaaag cagatgaacg ccattgaaaa aatcatctct cagatgaacg
    70021 aagcagcaga gcaggaaaga gcggcgttag aacaagaaga gcgaatgaag atcgatcaaa
    70081 actttgaaca aaaacgtacg caagttgaaa cagaacatca aaaacaaaaa gaaaaacaaa
    70141 tcgaattact cgaaaaaaaa taccgtcagc taaggaatcg ccagcaagta gaagtacgac
    70201 aagaaaattt gaatgcgaag caggaatttt taagacgatt gtttgcagat gcggtaactg
    70261 aaatggaaaa ttgggatgaa tctgaacaaa tccagtttat caaaaatgca ttgtattcat
    70321 tacctttgac aggaaaagta gcattcattg ctggagaaaa atcagcagcc tatctttctc
    70381 agacgttgtt ggatgaatgg aacaatgaac ttccctttat gatggtttta agtgatgaaa
    70441 ctgttgctga tcaagcgggt tttctgataa acgatcaagg agttcagtat aattttcttt
    70501 tcagcagcct tgtacaagat atccaaggaa cgatgagttt tgagattgcc aatcaattat
    70561 tcgaataagg agggatcagt gtatggaata ccacgaacta aatcccttga ttcgcggaag
    70621 agagcttgaa cttttgtcca atgagacatt tgaacggatg attcaaacaa attctgtcga
    70681 agcacttggt gaaatattga aatccacgat ttacagtcca tatatttatg acggatatga
    70741 atatgacttc gaacagaatc ttttaaagga gcagagccgt ctatttcgct ggttaaagga
    70801 atcagcaccg gagcctgaaa tcgtttggat ctatacgatg cgttatactt tccataatct
    70861 gaaggttttg acaaaagctg agatgacaaa tcaaaatctg gaccatttgt atatcgacga
    70921 tggattttat tcattagaaa ccttgaagga tgccattcat acgcaagttt caaatgaact
    70981 tcctgaaagc attatggaca gtatacgaga agtacgtgat tattttgaag agtctaccat
    71041 tcttcaggga attgacgtga tttatgatcg acagtttttg acagagcagc gacggcttgc
    71101 cgataagcta ggatatcaag aattgctgac agaagtcatt gcttttattg acttgaccaa
    71161 tatcacaacg atggctcgag gaatattgca gaaaagaaca tatgggttca tgacgacggt
    71221 actttccagc tcgggaagta tcgaaaaaga agtatttttg aactttgtag ataaagatat
    71281 ggaagattac acgaattttc tattagagac agattatgct gattttctgc gccctgtcat
    71341 ccgtgaggga aatattgatc tcgtaaagct tgaacagttg aaagatgatt atctaagttc
    71401 attgtatcaa gaagcacaaa cccaggcttt cggaccactg ccactactag cctttttgaa
    71461 cgcaaaagag gtagaaagca aaaaccttcg tttactggtt atcggcaaaa gaagccattt
    71521 cgaatcagaa acaatcagaa agcgggtgag acaagtctat gacgcataag atcggtgtag
    71581 ttggcgacaa agattctgtt ttgccattca aattgtttgg ctttgatgtt cgttatggta
    71641 caaccaagca aaccgttcgt caggcaattg aagaaatggc aaagaatgag tatggcgtga
    71701 tttatgtcac agagcaatgt gcagcattga gcccagaaac gatcgaacgt tacaaggaac
    71761 aaatgatacc tgctatcgta ttgattccca atcatcaagg atcattaggg atagggctgg
    71821 aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa tatcttatag tatgaaaagg
    71881 agatagaaat tttgcaaatt ggaaaaatca taaaagtctc cggacctctt gtaatggctg
    71941 agaacatgtc tgatgccagt atccaagaca tgtgtctagt gggagatcta ggagttatcg
    72001 gagaaattat cgagatgcgt ggcgatgtgg cgtctattca agtatatgaa gagacttctg
    72061 gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc tttatctgtt gaattaggac
    72121 cagggatcat ttcacaaatg tttgacggaa tccaacggcc attagatacc tttatggaaa
    72181 tcacacaaag taatttctta gggcgcggag ttcaattgcc cgcattggac catgagaaaa
    72241 aatggtggtt cgaaccaaca gtagaagccg gagaaacggt gagtgctgga gatgttatcg
    72301 ggatcgtaga agaaacaaaa gttataaaac ataaaatcat ggtgccaaat gggataaaag
    72361 gaacgatcaa aacaatcgaa aaaggttcat tcactattga tgaaacagta tgtgtcattg
    72421 aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa atggccagta agacgtagtc
    72481 ggccaatcaa agaaaaatta aacccggatg cacccatgat cactggacag cgagtgattg
    72541 atacattctt cccagtgaca aaaggaggag cagcagctgt acctggtcct tttggtgcag
    72601 ggaaaacagt cgttcagcat caaattgcaa aatgggccga tgtggacatg gttgtctatg
    72661 tgggatgtgg cgagcgtgga aatgagatga cggatgtttt aaatgaattc cctgaattga
    72721 ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt attgattgct aatacatcaa
    72781 acatgccagt agcagctcga gaagcttcta tctatacggg aatcacaatt gcggaatatt
    72841 tccgtgacat gggttatgac gttgctatca tggcagactc cacttcacga tgggcagaag
    72901 cacttcgtga gatgagcgga cgtttggaag aaatgcctgg tgatgaaggt tatccagctt
    72961 atcttggttc acggcttgcg gaatactatg aacgatctgg aagagtcatt gcattaggat
    73021 cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt atccccgtct ggaggggata
    73081 tttcggaacc agtcacacaa aatacattgc gggtcgtaaa agtattctgg ggtcttgatt
    73141 ccagtttagc ccaaaaaaga cattttcctt cgatcgattg gatacaaagc tattctcttt
    73201 attcaacaga agtagggaag tatatggatc atatccttca agctgactgg gcatccatgg
    73261 tcaccgaggg catgcgtatt ttgcaggaag aacagcagct gaatgaaatc gtgcgtttgg
    73321 tagggattga ttccctttca gataatgacc gtctgacatt agaagtggca aaatccattc
    73381 gtgaagatta tttgcagcaa aatgcttttg atgatgtcga tacattcact tcaagagaga
    73441 aacaattcaa aatgttgaag atgatcttga cctttggtga agaaggacgc cgtgcgttgt
    73501 cgttaggtgc ttatttaagt gagatcatgg acggtaccgt atcgattcgt gaaaggatcg
    73561 gacgaagcaa atatatcccg gaaacagaat tagaaaaact agatcaaatt attggtgaaa
    73621 tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat gagcaatgat taaagaatat
    73681 cgtacgatcg gtgaagtcgt cggtccattg atggcggttg aaaaagttgc tggtgtcaaa
    73741 tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga ttcgtcgtgg acaagtattg
    73801 gaagtccaag aagataaagc aatggtccaa atattcgaag gaacaagtgg tatcaattta
    73861 cgtgactctt ctgtacgatt tttaggccat cctttagaac taggtgtatc ggaagacatg
    73921 atcggtcgag tattcgatgg tttgggacga ccgaaagata acggaccaga gatcctgcct
    73981 gaaaagcatt tggatattaa cggagaagta atcaatccag tagctcggga ttatccagat
    74041 gagtttatcc aaacaggtat ctctgcaatc gatcatttga atacattagt tcgtgggcaa
    74101 aaattaccag tattttctgg ctctggtctt cctcataaag aattagctgc tcagatcgcg
    74161 cgacaagcaa cggtactcga ttcatcagat gatttcgccg tagtctttgc tgctattgga
    74221 atcacttttg aagaagctga atttttcatg gaagattttc gtcaaacggg cgcaatcgac
    74281 cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa tcgaacgtat cgctactcct
    74341 cgtatggctt tgactgctgc agaatatctt gcttatgaaa agggaatgca tgttttggtt
    74401 atcatgacag acatgaccaa ctatgcagag gcattgcgag agatttctgc agcacggcga
    74461 gaagttccag gaagaagagg atatcctggg tatctttaca cgaacttagc tactttattc
    74521 gaacgagcag gaagaatccg cggactaaaa ggatcggtta ctcaaattcc gattctaaca
    74581 atgccagaag acgataaaac tcatccaatc cctgacttga ctggttatat tacagaagga
    74641 cagatcatct tgactagaga gctttacaag agtgggattc agccaccaat tgatgtactc
    74701 ccgtctcttt cgcggttaaa agataaggga actggacccg gaaagacaag agaagaccat
    74761 gcagctacga tgaatcagct ttttgcagcc tatgcacaag gaaaacaggc aaaagaatta
    74821 gccgtagttt taggtgaatc tgctttatct gatgtagaca agatctatgc gaagtttgct
    74881 gagcggtttg aaaaagaata tgtcaatcaa ggattctaca cgaaccgttc gattactgaa
    74941 acacttgatc tagggtggga actgctatcg atgttgccaa gaaccgaatt aaaacgaatc
    75001 aaagacgaca tgctggacaa atatctgcca gaagggaagt gagttctgat ggcacgattg
    75061 aatgtgaatc cgacccgcat ggaattgact cgattgaaaa aacagttgac aactgccaca
    75121 agagggcata aattattaaa agataagcaa gatgaactga tgcgtcaatt catcctgttg
    75181 attcgaaaga acaaccagct gcgtcaagaa atggaagagt ctatgacaga agcaatgtct
    75241 gattttgtat tggcaaatgc ttcgatcacg gaacctttca tcgaagaatt atttgttttg
    75301 ccggcggaaa acgttgattt gtctatcgta gacaaaaata tcatgagtgt aaaagtacct
    75361 gtgatgaatt ttgaatatga tgaacagctg acagacacac ctttggaata cgggtatttg
    75421 aattccagtg ccgaactgga tcggtcaatc gatcggttta ctaccctgct tccaaaactt
    75481 ttggaactga cagaaatcga aaaaacttgt caactgatgg cgagtgaaat cgaaaagaca
    75541 cgtcggcgag tcaatgcatt agaatatatg acgatccctc aattagaaga aacgatctat
    75601 tatattcgta tgaagctgga agaaaacgag cgcgcagaag taacaaggtt gatcaaagta
    75661 aaaaatatgg gtaccactga ctaagtggta tcttttttta aaagaggaaa acgatgatta
    75721 aacaaagcaa acaaataaag aaacgtttgc cggctgttca gcttctcgca ttaggatttt
    75781 ttctcctgat tttagttggc ggcggcctac ttaccctacc attttttagt aatagtgggc
    75841 atgggacaaa ttttatcgat gcgcttttca ctgcaacttc tgctgtctgt gtaacgggat
    75901 tgacaacatt gaatacagca gagcattgga acagtgctgg tcaatttttg atcatgcttt
    75961 tgattgagat cggcggtctt ggttttatga tggttccgat tctatgcttt gcattgataa
    76021 agaagaaagt cagtctcagc acacgtatta tattaaaaga agcgctgaat ctagaagaag
    76081 tatcaggtgt gatgaagctc atgctttaca tcctaaaatt ggcctttgtg atccaattga
    76141 ttggtacggt tgctttatct tttgtatttg tgcctgaatt tggctggttg aaaggtatct
    76201 ggtacagttt gttccatgca atctctagtt tttgtaatgc agggtttgat ttgttaggag
    76261 acagtttagc caatcaccag caaaatgttt atttgatcat ggtggtatct gctttgatca
    76321 tttctggtgg gttaggcttc atcgtatggc gtgatttact ggaatatcat cagaaaaaga
    76381 agatgacgat tcattcaaaa atcgcattaa ccgttacagg taccctttta ttaggcggat
    76441 tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct agtggaaggg aatatgtttc
    76501 aacgattggc aaacaccttt tttatgagtg tgacacctag aacggctgga tattattcag
    76561 ttgattacct ccatatgagc catgcaggtc tgattttaac gatgtttttg atgtacatcg
    76621 gcggaacatc tgggtcgacg gccggcggat tgaaaacaac tacgttaggt gtgttgctga
    76681 tccagatgcg ttcgatgttg aaaggacgta cgagagcgga agcatttggc cgtaccatcc
    76741 gacaaggcgc agtgttgcgg gcacttactt tattttttgt tacgttgacg ttgtgtgtag
    76801 tcgccatcat gattttatcg gtgactgaaa cgatacctaa aacttctggc atcgaatata
    76861 ttgcttttga ggtcttctct gcgtttggta cagtaggtct tacgatggga ttgacgccag
    76921 acttgactgc aatagggaaa ttgatcatta tagcgcttat gtacattgga cgagttggtg
    76981 tattgactgt ggtcttctct ctgatgacaa aagcacaaag aacacaatca aaatacaaat
    77041 atccagaaga aagtgtcatg attggataat ttcatcttct gacagaaacc tagtgattta
    77101 gtaaaataag aaaataaaat aatttttctt gcttcttgga tactttaagt gcaaaatgtt
    77161 cgtttatttt ttgaggcact acccgttgtt gaacctcggg tagtgcctct tttttattga
    77221 aaggaatcaa taagatttga ttcctttatt tatcaatagg ttataattca tattttttta
    77281 ttttttcttt tacgatactt agtccagaag ctaaaatttc aaggttatta gaggataggg
    77341 aaattcgaac gaatgcatct ggttgcgcac gtgtagtaaa gcgtctggaa tgataaagtc
    77401 ggataccgtt agcaagaaag tcattctcaa ttatcttagg gtcaatctca ggatcaactg
    77461 gaagaattcg atagtaaggg acacgattcg ttggtttttc tactttgaaa agagaatcaa
    77521 aaagctgatt ggctcttttt gtaagactta atttagtttc taagatccta tttgcttcat
    77581 ttgaatggag gatttgagtg atgacttcca catctaatcc agaagttttc acatttatat
    77641 taaaaattgc ttgttctatc ttgctttgga catgtactgg aactacaaga taagcaactc
    77701 gcaatccaga acaaataaat tttgtcattc cagctaagta gattgtttgg ttaggtaata
    77761 aattttggaa aggaggaatg atcgtatgtt gatagtaagt tgttaaaaat gaatggatat
    77821 catcttcaat aacccataga ccttcttttt caatcacttc ggctaattgc tctcgtcttt
    77881 tgtgagagat ttgaaaccct ataggattat tacaagaagg cataagaaag acaccatgaa
    77941 tcttcttttt tctacattcc tgaatcaata tatcaggtcg cattccttgc tcgtcaaaat
    78001 caattggaac gatctctaaa tgatataact gagccaattc aatgaaatta gcgtatgtat
    78061 atcggtctgt tgctattcta tcacctggag agaaaagtgc agaaagtgtg atagccaatc
    78121 cattttgaac accggaaaca atagatagag attctaagga cggaatgact ccttgagttt
    78181 ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc tttgggatct tggtaattaa
    78241 gcaaaccaac aagatttggg ttattactag ttgattgaat aaacgggaca acgaatttat
    78301 tacattcttc aaaagaacta accagaccaa aatcaatgat atgttcggtt acttcattgg
    78361 tagagatggt gatggactca aagacattgg gagaaataaa tgtgccactt ccaatattcg
    78421 tatacagcaa acctttctca attccatatt tataagcctt gccaacggta gtaaaattta
    78481 aatctaaata atcagctagc tctctttggg agggtaactg agtgtttttc tgtaatttat
    78541 tctttaaaat atcttgttct aattgttgaa tcaacgatcg ataataagga cgcttcaatt
    78601 tttgtttgtc tggtcgccaa gttaacgggt aattgtcata agaattaata ggcattttct
    78661 ccactcctta aaattgtata ccatacaatt ttaccattga ttgtattgat tttagaagtt
    78721 aatcttttgt gaggagggaa ttgtctatga aaattattaa ttttgaggaa gtagtagctt
    78781 cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg tttttttgat tatgaaagtg
    78841 gtaaaatcag ccaaaatcct cgattggtgg aaattttacc tgacggaaaa ggcaaaaata
    78901 tgtttgcgat gatgcctgct tatttggggg agaacagata ttttggcaca aaagtcatta
    78961 ccgcttttcc ggataatcat cagtatcatt taccttctca tttaggagag attttcttgt
    79021 ttgatgctca aaacggacta cccaaagcat tgataaacgc aaatgcagtc acctggattc
    79081 gaacagctgc tgtatctgcg ctggcaacag attacttagc aaatccgcat gcagaaacac
    79141 ttgctttaat tggagctggt caacaagcat tgtcgcattt gactgcgatt tctaccattc
    79201 gtcccatcac tacagtgttt gtttacgata ttttagaaga aagacgaaat acttttatcg
    79261 ctaaagctaa aaaacagtat cctaaaataa cgtttataaa tgcaatatca gtagaagatg
    79321 cagtacgtcc agcggaaatc atttgtacgt tgacctctag taaagaggca tttcttcaaa
    79381 aagagtgggt aagggaagac gcacatatca atgctgtggg cacatttact cctgatacaa
    79441 gagagataag tacatcgttg atggaaactt ctcaaatcta tgtagatgat tactcagctg
    79501 ccttaacaga aagcggggac ctgcttatag ccatggcaga aggagattta gaggaatctt
    79561 gtattgttgg tacattaggc gaattagttt ctgagaaaaa aacattttat aaaggagaat
    79621 cgaggccgac gattttcgac gcagttggtc tagctattga agatctttgt tgtgcagaat
    79681 atatttataa caaattagag aaaggggctt taaaatgaac ttagattatt acgtagttga
    79741 tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg gtttatgtgc tagaagaatg
    79801 gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac aatttatctg aaacagcatt
    79861 tacagtgaag aaaaaccaag aatttgagct tagatggttt acgccagatc gagaaatcga
    79921 tctttgtggt cacgcgacat tagcaacagc atttgtttta tttaactatt acaaaatacc
    79981 tgatgaaacg attaaatttt ctactcaaag cggtaatcta tttgtgacaa gaattaaaga
    80041 ttattattac atggattttc ctagtattat gccgaaaaaa gtgccgatat tgtctgaata
    80101 tgaagaagcg attggggcta agattaaaga agcctactta gcacgtgatt tatttttcgt
    80161 attagaagat gaaaaaacgg tggctaaatt gcagccagat tttacagcta tcaaaaattt
    80221 tgaattagga ataggagtca tcgtgacagc agaaagtgtg caaaaagact ttgtttctag
    80281 aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag cacattctaa
    80341 tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc
    80401 atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa
    80461 agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg aattaaaaat
    80521 aaattggtgg agagtggcaa ccttatagct acaatggttg ttaaaaaaag acgcaaactt
    80581 tttcaattta atgaaaagtt tgcgtctcat tttaattatt ggtgtttgtt ttttattcaa
    80641 tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta taaccatcag
    80701 ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa tccttttaag tcagtaatct
    80761 caagaccagc agctttatgt tgctcgtgta atttttctag atcatcagct gctatagcga
    80821 tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa gtcaactcta
    80881 attcatagtc atccccagga agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat
    80941 cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt gatgcttcaa
    81001 gatctttcac tcgaacacat gtgtgagcca ttttcataga ttcatctctc cttttccttt
    81061 tactcaatca taacaaaaag aagtaaggaa atagttgatt tgtgcttgtt catgagaaat
    81121 ttctcatgta aaatggatag aaaataaaac tgttaaggtg gaaactattc atggataaac
    81181 caatttttgg tgaaaaaaag caatttgctg attatcaaac ccgttatgca gcatatattg
    81241 tcatagaaag agatcagcag attgcagtaa tcgaggctcc aaatggtgct ttttttcttc
    81301 ctggcgggga aattgaagga gaagagacga aagaagaagc gattgtccgt gagctgatag
    81361 aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat gagtattttt
    81421 actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt gctgattctt
    81481 ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg agccctcaag
    81541 aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga tggatcgaaa
    81601 aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt tatttattac
    81661 agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca
    81721 tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag accagcatat
    81781 tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc ttgatcaata
    81841 ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt atttagtact
    81901 tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa
    81961 cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt gacgatgttc
    82021 atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac taaacttgct
    82081 tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc
    82141 gaatggagat gactgccgac acggcgtgcc attacttcga tctctaggaa atcttcatgt
    82201 gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag tgccccgtta
    82261 taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg ttcctggaca
    82321 cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac agcagcggca
    82381 atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt accatcgatg
    82441 tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt tatttcaatc
    82501 aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa taaatcataa
    82561 tgatcacgca aggacggatc aaccatttct tttggaaaga aaaatcgctc atctccttgt
    82621 tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat caaagttccg
    82681 tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca
    82741 taaggtagat cgtagctaga attcacagca gtgtaataaa caggattata tccgaccttt
    82801 tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga aaaagtagca
    82861 gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat aggatcagca
    82921 gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt gaggtattct
    82981 tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag ttcaaaactg
    83041 ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa aattacttcc
    83101 ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg gctaacgata
    83161 taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc ataaggacga
    83221 atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt gaaataagca
    83281 tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat ttggacaact
    83341 tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa accttcttct
    83401 actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc tacagtgatg
    83461 gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc atgaccgaca
    83521 tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca gcctccgttg
    83581 cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct tgaaatctca
    83641 taaacgccta atgaatgagt aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa
    83701 gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt tcgaattgat caaatctaag
    83761 attacttgat gctggacatg gatatagtta tggactggat cacgaaaaac tttttccata
    83821 ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt tattacgttg
    83881 ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt ttagccagtc
    83941 ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg caaatgcttc
    84001 tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg ccattgtagc
    84061 aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag attgataaaa
    84121 tcgcttcatg acttgcccac gtttttcctg attaccgttg acacttaagt acatcataac
    84181 agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc catggatgct
    84241 taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta tcggtgcctt
    84301 tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta tggttatttt
    84361 ttttgtgtca ctttggggga aaatcaaaga aatattcaaa attccttcat ctgatacgat
    84421 ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatcttcca gtgtttggat
    84481 cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg tgcagtcata
    84541 ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta atatatctgt
    84601 taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg
    84661 gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat actctaaaat
    84721 caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt tgacaaaaca
    84781 aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat ggaattatca
    84841 caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa
    84901 gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct gtacatacgt
    84961 tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa attagttcca
    85021 gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat
    85081 aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat tgctactttt
    85141 acgaaagaac tgcatgttag tttgaaagac cgtcccacag cagggaaggt tcgaatcgat
    85201 tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt ggattttaca
    85261 gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat aaggagtaga
    85321 ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa
    85381 ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt catggatttt
    85441 tctgatttag ctaaccagat ccaaaactat cttgggaaat cagacagtga aattcgtgat
    85501 tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc attaggggat
    85561 aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga agtaaaccat
    85621 ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa agtcaacgcc
    85681 tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga agatacagat
    85741 ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga tgaagaaatc
    85801 gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga tgatgaagaa
    85861 gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga tgatgatgaa
    85921 gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt ttcttacgag
    85981 aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacga tttttgtcac
    86041 accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa aaattgaaac
    86101 ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga atgattttta
    86161 ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat ggactcatcg
    86221 aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat aaagaagagg
    86281 ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa aaagaaaaag
    86341 agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca ttagatagtt
    86401 ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc ccccaacagt
    86461 tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata gacagtccgg
    86521 aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa attgcgacta
    86581 tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg aatgctttac
    86641 gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga aaatttaaaa
    86701 aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga ggagatccgc
    86761 cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa gagacgtctc
    86821 caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct tctttttaat
    86881 agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat tgaattgatc
    86941 ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc gaagatcaat
    87001 acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata ctttcgcgaa
    87061 ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac ggatcattac
    87121 caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt gattgaaagg
    87181 cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac aatcgacaag
    87241 aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta tctatctgtt
    87301 ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc atctaagtac
    87361 tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact tgttacgcct
    87421 acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc tcaatacgaa
    87481 attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt gacaaaaacg
    87541 attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga ctagcagttt
    87601 caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga agagatatag
    87661 tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg tttttcgaaa
    87721 ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct tcggagcatt
    87781 cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca actgaaacag
    87841 ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag tcatcacaat
    87901 caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc gcaatcagct
    87961 ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta aaagaaaaaa
    88021 acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct gaaatcgtga
    88081 agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat cagaatctgg
    88141 ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact gagatcaaaa
    88201 aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc acgaatgtag
    88261 gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa gattggaaga
    88321 caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat ggggcaatta
    88381 ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg atcgattatc
    88441 tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat ccaacggctg
    88501 tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga caagtatctt
    88561 taataaataa cacattttaa taagtagttg aacacataac taccttactt gcaaaggctt
    88621 aacaatatct atacaggtac aacaaaaaac tgtccaccaa tttt
//
LOCUS       DASJSZ010000002        75657 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.002, whole genome shotgun sequence.
ACCESSION   DASJSZ010000002 DASJSZ010000000
VERSION     DASJSZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 75657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.002"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..456)
                     /gene="rlmH"
                     /locus_tag="U4B03_000092"
     CDS             complement(<1..456)
                     /gene="rlmH"
                     /locus_tag="U4B03_000092"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEN2066972.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRIN"
     gene            884..2173
                     /locus_tag="U4B03_000093"
     CDS             884..2173
                     /locus_tag="U4B03_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEN2066973.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2212..2817)
                     /locus_tag="U4B03_000094"
     CDS             complement(2212..2817)
                     /locus_tag="U4B03_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEN2066974.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3022..3486)
                     /locus_tag="U4B03_000095"
     CDS             complement(3022..3486)
                     /locus_tag="U4B03_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2066975.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3508..3828)
                     /locus_tag="U4B03_000096"
     CDS             complement(3508..3828)
                     /locus_tag="U4B03_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN2066976.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(3972..5051)
                     /gene="pepA"
                     /locus_tag="U4B03_000097"
     CDS             complement(3972..5051)
                     /gene="pepA"
                     /locus_tag="U4B03_000097"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEN2066977.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5251..5601
                     /locus_tag="U4B03_000098"
     CDS             5251..5601
                     /locus_tag="U4B03_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN2066978.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            5824..6771
                     /locus_tag="U4B03_000099"
     CDS             5824..6771
                     /locus_tag="U4B03_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2066979.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(6828..8246)
                     /gene="pepV"
                     /locus_tag="U4B03_000100"
     CDS             complement(6828..8246)
                     /gene="pepV"
                     /locus_tag="U4B03_000100"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEN2066980.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            8395..9240
                     /locus_tag="U4B03_000101"
     CDS             8395..9240
                     /locus_tag="U4B03_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEN2066981.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            9244..9684
                     /locus_tag="U4B03_000102"
     CDS             9244..9684
                     /locus_tag="U4B03_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN2066982.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            9734..9928
                     /locus_tag="U4B03_000103"
     CDS             9734..9928
                     /locus_tag="U4B03_000103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2066983.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            10064..10741
                     /locus_tag="U4B03_000104"
     CDS             10064..10741
                     /locus_tag="U4B03_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2066984.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(11149..11838)
                     /locus_tag="U4B03_000105"
     CDS             complement(11149..11838)
                     /locus_tag="U4B03_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2066985.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(11813..13942)
                     /locus_tag="U4B03_000106"
     CDS             complement(11813..13942)
                     /locus_tag="U4B03_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEN2066986.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(13990..15327)
                     /locus_tag="U4B03_000107"
     CDS             complement(13990..15327)
                     /locus_tag="U4B03_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEN2066987.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(15579..15938)
                     /gene="rplT"
                     /locus_tag="U4B03_000108"
     CDS             complement(15579..15938)
                     /gene="rplT"
                     /locus_tag="U4B03_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEN2066988.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(15991..16191)
                     /gene="rpmI"
                     /locus_tag="U4B03_000109"
     CDS             complement(15991..16191)
                     /gene="rpmI"
                     /locus_tag="U4B03_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEN2066989.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(16263..16787)
                     /gene="infC"
                     /locus_tag="U4B03_000110"
     CDS             complement(16263..16787)
                     /gene="infC"
                     /locus_tag="U4B03_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEN2066990.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(16831..16956)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            17107..17739
                     /locus_tag="U4B03_000111"
     CDS             17107..17739
                     /locus_tag="U4B03_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN2066991.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(17741..18646)
                     /locus_tag="U4B03_000112"
     CDS             complement(17741..18646)
                     /locus_tag="U4B03_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2066992.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(18731..19390)
                     /locus_tag="U4B03_000113"
     CDS             complement(18731..19390)
                     /locus_tag="U4B03_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2066993.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            19706..20650
                     /gene="mvk"
                     /locus_tag="U4B03_000114"
     CDS             19706..20650
                     /gene="mvk"
                     /locus_tag="U4B03_000114"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEN2066994.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            20643..21620
                     /gene="mvaD"
                     /locus_tag="U4B03_000115"
     CDS             20643..21620
                     /gene="mvaD"
                     /locus_tag="U4B03_000115"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEN2066995.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            21633..22718
                     /locus_tag="U4B03_000116"
     CDS             21633..22718
                     /locus_tag="U4B03_000116"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEN2066996.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            22715..23779
                     /locus_tag="U4B03_000117"
     CDS             22715..23779
                     /locus_tag="U4B03_000117"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEN2066997.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(24028..24831)
                     /locus_tag="U4B03_000118"
     CDS             complement(24028..24831)
                     /locus_tag="U4B03_000118"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEN2066998.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(24851..25693)
                     /gene="kduI"
                     /locus_tag="U4B03_000119"
     CDS             complement(24851..25693)
                     /gene="kduI"
                     /locus_tag="U4B03_000119"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEN2066999.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(25709..26716)
                     /locus_tag="U4B03_000120"
     CDS             complement(25709..26716)
                     /locus_tag="U4B03_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN2067000.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(26723..27367)
                     /locus_tag="U4B03_000121"
     CDS             complement(26723..27367)
                     /locus_tag="U4B03_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN2067001.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(27467..28225)
                     /locus_tag="U4B03_000122"
     CDS             complement(27467..28225)
                     /locus_tag="U4B03_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEN2067002.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(28352..29503)
                     /gene="fucO"
                     /locus_tag="U4B03_000123"
     CDS             complement(28352..29503)
                     /gene="fucO"
                     /locus_tag="U4B03_000123"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEN2067003.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(29585..29896)
                     /gene="rhaM"
                     /locus_tag="U4B03_000124"
     CDS             complement(29585..29896)
                     /gene="rhaM"
                     /locus_tag="U4B03_000124"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEN2067004.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(29898..30743)
                     /gene="rhaD"
                     /locus_tag="U4B03_000125"
     CDS             complement(29898..30743)
                     /gene="rhaD"
                     /locus_tag="U4B03_000125"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEN2067005.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(30757..32013)
                     /gene="rhaA"
                     /locus_tag="U4B03_000126"
     CDS             complement(30757..32013)
                     /gene="rhaA"
                     /locus_tag="U4B03_000126"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEN2067006.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(32010..33470)
                     /gene="rhaB"
                     /locus_tag="U4B03_000127"
     CDS             complement(32010..33470)
                     /gene="rhaB"
                     /locus_tag="U4B03_000127"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEN2067007.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            33623..34492
                     /locus_tag="U4B03_000128"
     CDS             33623..34492
                     /locus_tag="U4B03_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEN2067008.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(34618..35517)
                     /locus_tag="U4B03_000129"
     CDS             complement(34618..35517)
                     /locus_tag="U4B03_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2067009.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(35682..36506)
                     /locus_tag="U4B03_000130"
     CDS             complement(35682..36506)
                     /locus_tag="U4B03_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2067010.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(36517..37812)
                     /locus_tag="U4B03_000131"
     CDS             complement(36517..37812)
                     /locus_tag="U4B03_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEN2067011.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(37828..38307)
                     /locus_tag="U4B03_000132"
     CDS             complement(37828..38307)
                     /locus_tag="U4B03_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEN2067012.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(38313..39341)
                     /locus_tag="U4B03_000133"
     CDS             complement(38313..39341)
                     /locus_tag="U4B03_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEN2067013.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(39358..40464)
                     /locus_tag="U4B03_000134"
     CDS             complement(39358..40464)
                     /locus_tag="U4B03_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2067014.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYY"
     gene            complement(40693..43290)
                     /gene="adhE"
                     /locus_tag="U4B03_000135"
                     /gene_synonym="adhC"
     CDS             complement(40693..43290)
                     /gene="adhE"
                     /locus_tag="U4B03_000135"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEN2067015.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(43595..43975)
                     /gene="yajC"
                     /locus_tag="U4B03_000136"
     CDS             complement(43595..43975)
                     /gene="yajC"
                     /locus_tag="U4B03_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEN2067016.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(44072..45217)
                     /gene="tgt"
                     /locus_tag="U4B03_000137"
     CDS             complement(44072..45217)
                     /gene="tgt"
                     /locus_tag="U4B03_000137"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEN2067017.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            45435..46133
                     /locus_tag="U4B03_000138"
     CDS             45435..46133
                     /locus_tag="U4B03_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN2067018.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(46301..48940)
                     /locus_tag="U4B03_000139"
     CDS             complement(46301..48940)
                     /locus_tag="U4B03_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2067019.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(49559..50590)
                     /gene="queA"
                     /locus_tag="U4B03_000140"
     CDS             complement(49559..50590)
                     /gene="queA"
                     /locus_tag="U4B03_000140"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEN2067020.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            50783..51958
                     /locus_tag="U4B03_000141"
     CDS             50783..51958
                     /locus_tag="U4B03_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2067021.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            51962..52603
                     /locus_tag="U4B03_000142"
     CDS             51962..52603
                     /locus_tag="U4B03_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2067022.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            52600..53517
                     /locus_tag="U4B03_000143"
     CDS             52600..53517
                     /locus_tag="U4B03_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2067023.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            53514..54185
                     /locus_tag="U4B03_000144"
     CDS             53514..54185
                     /locus_tag="U4B03_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2067024.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(54726..55214)
                     /locus_tag="U4B03_000145"
     CDS             complement(54726..55214)
                     /locus_tag="U4B03_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEN2067025.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(55415..55681)
                     /locus_tag="U4B03_000146"
     CDS             complement(55415..55681)
                     /locus_tag="U4B03_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN2067026.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(55681..55944)
                     /locus_tag="U4B03_000147"
     CDS             complement(55681..55944)
                     /locus_tag="U4B03_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2067027.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            56420..56605
                     /locus_tag="U4B03_000148"
     CDS             56420..56605
                     /locus_tag="U4B03_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2067028.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(56747..58570)
                     /locus_tag="U4B03_000149"
     CDS             complement(56747..58570)
                     /locus_tag="U4B03_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN2067029.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(59168..61873)
                     /locus_tag="U4B03_000150"
     CDS             complement(59168..61873)
                     /locus_tag="U4B03_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEN2067030.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(62179..63126)
                     /locus_tag="U4B03_000151"
     CDS             complement(62179..63126)
                     /locus_tag="U4B03_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEN2067031.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            63316..63585
                     /gene="rpsN"
                     /locus_tag="U4B03_000152"
     CDS             63316..63585
                     /gene="rpsN"
                     /locus_tag="U4B03_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN2067032.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(63761..64123)
                     /locus_tag="U4B03_000153"
     CDS             complement(63761..64123)
                     /locus_tag="U4B03_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2067033.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(64134..65255)
                     /locus_tag="U4B03_000154"
     CDS             complement(64134..65255)
                     /locus_tag="U4B03_000154"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEN2067034.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(65271..65621)
                     /locus_tag="U4B03_000155"
     CDS             complement(65271..65621)
                     /locus_tag="U4B03_000155"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEN2067035.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(65751..67262)
                     /locus_tag="U4B03_000156"
     CDS             complement(65751..67262)
                     /locus_tag="U4B03_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2067036.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(67584..68936)
                     /locus_tag="U4B03_000157"
     CDS             complement(67584..68936)
                     /locus_tag="U4B03_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEN2067037.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(69089..70165)
                     /locus_tag="U4B03_000158"
     CDS             complement(69089..70165)
                     /locus_tag="U4B03_000158"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEN2067038.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            70361..72106
                     /locus_tag="U4B03_000159"
     CDS             70361..72106
                     /locus_tag="U4B03_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEN2067039.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(72386..72823)
                     /locus_tag="U4B03_000160"
     CDS             complement(72386..72823)
                     /locus_tag="U4B03_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEN2067040.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(72807..74180)
                     /locus_tag="U4B03_000161"
     CDS             complement(72807..74180)
                     /locus_tag="U4B03_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067041.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(74502..74990)
                     /locus_tag="U4B03_000162"
     CDS             complement(74502..74990)
                     /locus_tag="U4B03_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2067042.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(75012..75614)
                     /locus_tag="U4B03_000163"
     CDS             complement(75012..75614)
                     /locus_tag="U4B03_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2067043.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        21681 a        16186 c        13880 g        23910 t
ORIGIN      
        1 attgatccgg aaggcacggt agatttgctc gacaaggatc agacgcatca attgatgtgg
       61 atacgtcatt tttccaaaag agatctgagc attgcttctt ttcatgacgg cttcgctgag
      121 tcctaaagag ccgccgataa caaaaacaag gtggcttttt ccttggatac ctaattggtc
      181 gatttgttta gcaaaagatt cacttgtagg atttttccct tcaattgcta aggcatagac
      241 gtattcttgt tctttgattt tcgctaagat ccgttcccct tctttttctt ttacttgtct
      301 catttgcgct tcgcttaaat tctccggggc tttttcgtca ggaacttcga tcaattcgat
      361 ttttgcatag gcattcagcc gtttgacata ctcattgata ccttggatca ggtatttttc
      421 ttttaatttt cctactgaga taattttgat attcatgttt tctctccttt ggtcttcatg
      481 ctcactttgt ttattataat caattttttc gtctatatgt ttattttggc tatttttgtt
      541 aaaaagattt ccccaacagt tatgcacaac atccacaggt ttatccacag aaaacagtgt
      601 tattttacct tatttcatca cattcattag gcaaacatac gttttataag aatttatcca
      661 ctttatacac aggctgtgga tatgttttat cccttatttt tccaatccta tccgcgtagt
      721 tttacacagt atctgtggaa aaagaaaatc aaatattttc atctgaaaac aatgattttt
      781 cctaaagttt ttcataaaaa gttcacagtc tcaaagtaga ctaaagtcaa caaataagac
      841 tatactaaca gtagataaat gaatgtaatt ggaggaggaa cacatggata gaaaaaatgt
      901 gacaccaaag atgaaaaaaa gtaaaaataa tagcctctgg cgtaagttag gtctggggct
      961 tgttggaggg atcgttggag gactactgac agctggcatt ttttatgcag cgatgggcac
     1021 tggcaacaat agttctactg ctacaagcgg caatcagaat tctgccggtg aaacagttgt
     1081 ggaaaatgtc aaagtcaatg tagattctga tatcaccaaa gcggtagata aagttcaaga
     1141 cgctgtcgtt tctgttatca acttacaaag tcaaaaccaa tcaagcggtt ttggcggact
     1201 attcgggcag caagaagaaa gtagcagctc agatgatagt aatttggaag cttatagtga
     1261 aggaagcggc gtgatctaca aaaaagacgg gaatactgct tatgtagtca cgaataatca
     1321 cgtagtagat ggccagcaag gtcttgaagt cttgatgaaa gacggtacga aggtaaaagc
     1381 tgaattagtt ggaacagatg cttattcaga tttagcggtg ttgaagatca attcagacaa
     1441 agtcgaaaca gttgcctctt ttggtgactc aagtgctctg aaggttggtg agcctgcgat
     1501 tgcgattggt tccccactag gatctgaata tgcaaattct gtcacttcag gaatcatttc
     1561 ttccttgaac cgacaagtga caagtacgaa tgaatcgaac gaaacagtca atatcaatgc
     1621 gatccaaaca gatgcagcaa tcaaccctgg aaattctggt ggtcctttag ttaatatcga
     1681 aggacaagtt atcggtatca actcaagtaa gatcgcaagt acttctgaat ctacttctaa
     1741 tgtaagcgta gaaggaatgg gatttgctat tccaagtaac gatgtagtga atatcatcaa
     1801 tcaactggaa aaagacggaa aagtgacacg tccagctcta ggtatcacaa tggtcgactt
     1861 atctgccgtt tctactcagc aacaagaaca aatcttgaag attccttcat ctgtaactaa
     1921 cggtgtcatc gtgacaaatg tccaaacagc tacaccggct gaaaaagctg gattgaaaca
     1981 atacgacgtc attactaaga tcgatgacaa agaagtttct tctggtgtag aacttcaatc
     2041 tgctttgtat aagaaaaaag tgggagattc tgtcaaaatc acttactatg aaggctcgaa
     2101 agaaaaaaca actactgttg aattgacgat cgatcaatcg gcattaaaac aaaatcaaaa
     2161 tagtgaaaat taataaaaaa gaggttgtga caaagacttg tcacaacctc tttattttgg
     2221 aaatgcttct ccgactacgg catcctcagg caatactaag attccttttt ttgctggagc
     2281 atttggcaaa tgcaattcac gagcggaaca gatcattcca tagctatcta cccctcgcaa
     2341 agttcctggc cagatcatca ttccatctgg catcatagct cctggttttg caacgacaac
     2401 tttttgtcca gcttcgatat tgggtgcacc gcaaacgatt tgcaatactg taccattgtc
     2461 tacttctgtc tcagtaatcg ataaatggtc agaatcagga tgtgggacgc atgtttttac
     2521 gtacccgaca acgatcttcg gttcatgatc agcttcaagc tgttcttcga atcctgcttc
     2581 tttgatcaat cgattcaatt tcgctaattc ctcatctgtt aagcttactt ggccgttccc
     2641 ttcgattgtt agatgatcgg agatatggaa aaagttccaa gcaacaactt gaccttgttc
     2701 atctttgatc cgagcaacat tccatttgcg ttcaacagtg ttttctgttc ctttatcgtc
     2761 ggctataata accatcaagg tgtctccgac gtgtgctttg ttataagcaa aaatcatgtt
     2821 ctttcctcca ttatcctttg ctgagattta tcttaacaaa aaattgcatt cactgcatga
     2881 aatttttttc atgctttatt tccgaagaag cctcttctgg aacaccattt attcgtaaca
     2941 agggactgtg acaagacttt tgtcacagtc cctttactgt tttagttaat tgattaaaaa
     3001 ggtcgtacaa gttccacttg cttaattact tggctgttct tcttcgtcag aaacgatcaa
     3061 gacatcacaa ggtgaatgac gtatcacgta gctggctaca cttcctgtca taaagcgttc
     3121 gactgcattt agacctgact gcccaaccat gattagatcg atctcatatt tttttggcaa
     3181 tttgactgcc atggattcac gaacagaacc atatgcaata attccttcta tatttgtgta
     3241 atcaacagat ttcccatatt ctttacattc ttcgatcatt tccttggctg tttcagtttg
     3301 ctgatcgact agattttgat caataggtgc atagcccata aagttataaa attgctgatc
     3361 gattacgttt gctacatata ctttcccatt attacggcga gccacttcta ccgctttttt
     3421 gaaagcagca agtgcttgtt cactaccgtc gatacctact aaaatattct tatattcttg
     3481 attcatcttg acccctccta acagatgtca gccttttgtt acactttcta ggaacgattc
     3541 gatttcctgt tttgttttac gatctttatt cactagacga cctaattctt ttccgtcttg
     3601 gatcacgaca aaacttggga taccaaaaat gttccattca gcggctacat ctatgtactt
     3661 gtcacggtct acttcaataa attgccaatc agaaaagtca ttttcaattt caggcatttg
     3721 cggtttgata aaacggcagt ctccgcacca gccggcagta aagaaaaata cactctttcc
     3781 agtttctacg tagcctgcta attcttctaa actatttggg ataatcattc tttgtcctct
     3841 actttctgtt tatgtatttt tcaattaaat tatacacctt tctttcgaac taacaaacat
     3901 tgatcctctt tttataaaaa aaatctggca aaaagcaatc ttttgccaga ttttatagtt
     3961 tcactatcgc attattttcc gtatacgatc gtatttactg ttgatttatc caaacctttc
     4021 agtgtttgaa tcaacatttc tcgtgcagct tcaaaatcgc gaatactgaa catcgtttga
     4081 tgcgtatgga tataacgtcc cactacgcca atcactgtac ttgggattcc ttcgttttgt
     4141 gtatgagcag cacctgcatc tgtaccgcct tttgaaacaa agtattgata agggatattg
     4201 tttgtttcag cgatatccag caagtactcg cgcaaacgag gaagcatgat caaacctgga
     4261 tcttggatac gcatcaatgt accttctcct aaatgtccga atgtgccatt tttcgtcact
     4321 gtatcatctg ctgcagagca gtctactgca aagaacagat ctggcttgaa tttattgaca
     4381 gaagctttag aaccacgcaa gcctacttct tcctggacat tcgcgcctgc aatcaatgta
     4441 tgacctaatg tttcattttg caacgcttct aaagcttcta ataccattgt acagccatag
     4501 cggttgtccc atgatttact gataatattc tttccgttgg ctgttttgat cgtttcagcg
     4561 taaggaacga ttgtgtctcc tggaaggaca ccgtaactca ttgcctcttc tcttgattca
     4621 aacccagcat cgaacaaaat atcggttact tctactgatt tttgtccgct tgttcctcgc
     4681 aataaatgtg ggggaacgga agaagaaata catggataat tcttaccagt agaggtttta
     4741 agtgtaaaac gctgagcaga aacaacgtaa ggattccagc cgcctagagg tacgacttta
     4801 aaaagcccgt tttcttggat ttgtgtcagc atgaatccga cttcatccat atgtgctgcg
     4861 accattacac ggggtgcatc cgcttcttca tgatggcgca gaccaaaaat gccgcccaaa
     4921 ccatctaact gtaactcatc taccagcggt tccatatgac tttccatata agcgcgaaca
     4981 tcttgttcaa aaccgcttgt tccttgtaat tctgttaact ctttgattcg ttgaaatgtt
     5041 ttttcttcca tatgctattc gtctaaattt cagactaaac tcctctcaga cttatattga
     5101 tctgcggata aaatatgatt ttttctgcag accgatcgta tttattatag cttatttttt
     5161 ctggtagcgg ttatttattt caaagaaaat atgagagtgt ggtaaaataa gcacaaggag
     5221 taacaagtga gaagaaggag gtatgtccac atgtatgaag aaagcgaact aagctatttt
     5281 aaaggcgggt tagcagttgg aatcggtcta ggattattga gcggggtagc ttcaacgatt
     5341 tggtatcatc gcaaaaaagt attgtcagca gaccaagtac ttgaaaatat caagtcatct
     5401 tttctaaaag aaggtgcaat cgaaggttct tggatctctt ttgaaaagga acctattagg
     5461 aaattcgccg ttcggtcgag tgcttatcgg ggaggtcttt ctcgaataga agatggcgaa
     5521 actgtttatt atgaattttt agcagatgct catacaggaa ccgtattaga aatttcaaga
     5581 aaaaaaggtg caacagatta atacgtcaaa aaagtgagag aacgaatacg cacccactat
     5641 tcaaaagaga ggtatcatgt ccttcttttg tagtgggtgg ttttttagat agagctttcc
     5701 aaacatatat tccagtattt tctttttaaa taaatgatat ttagcgggaa aaactagcat
     5761 ttcctttgat tatgaggtat ttttaattta gcttttgttt tctatagtta aggagagtga
     5821 aaaatggaac aacaagaaaa aattgctgtt ctgattcctt gttacaatga ggaagcaaca
     5881 atttcaacag tcattgcaga ctttaaacga gaattaccag aagcagatat ttatgtctat
     5941 gataataatt cgaccgatcg aacatacgaa ttggccgttc aaggtggagc aatcgtaaaa
     6001 aaagaacctc gtcaaggaaa aggaaatgtg atccgccaaa tgttctttga tatcgatgcc
     6061 gactattatt taatggtaga tggcgatgat acttatcctg cagaagctgt acatggtttg
     6121 ctcgagaaat tacgttctgg tgaagcagat atggtcattg gtgatcgcct atcaaatgga
     6181 acctactttg atgagaataa gagacctttc cacgattttg gaaataacct tgttagaaat
     6241 acaattacta gaatgtataa aacaaaaatt cttgatgtca tgaccggcta tcgaggattc
     6301 aatcgtatct ttgtcaaaag ttttcctatt atgagttctg gttttcaaat cgaaacggaa
     6361 ttgacgatcc atgctttaga taaaaaattc aagttcgttg aaatcccaat cgattatcgt
     6421 gatcgtcctg aaggcagcga atcaaaattg aatacattct ctgacggatt caaagtgatc
     6481 atgatgatta ttaaaatgtt taaagattat aagcctttgc ttttctttgg gatttggacc
     6541 atcttcttct ttttatttgg attggtcacg gggattcctg tgattcgcga atatatgctg
     6601 acaagcttta ttacacgcat cccttctgcg attttaagta ctggattgat gactttagca
     6661 cttctttcat tagtaacagg gttgattttg gatacagtcg tcacgaacgc taagaaagaa
     6721 tatgaattga atctatatca tgtttatcat gaataccaca gtagaaagta aaaaaaggat
     6781 caagactgca aaaataattt gcaattcttg atcctctttt taattaatta tttcgccaaa
     6841 cgatagatgg catctgcata gattgctgtt gctctgaaca agtcatcaag actcataaat
     6901 tcgtttgctt gatgcattgt atcagtatat cctgggaaca tcgctccata agcaacgcca
     6961 cgtttcaaca aacgtccgta agttccgcca ccgatgattt gttcgtagcc tttttctcca
     7021 gtgtggtctt catagacttg caataatgta gccaccaatg gatcatccat tggtacgtaa
     7081 tgtggttcca ttacgcgagc acctcgagta actgttgcgc cttcttgccc cactgtttgt
     7141 tcgatgtcac tttgcaatcc gtctactgtt acaccttttg gaaaacggaa attcatattg
     7201 atgaagttcg cttcctctgt accattttct tcaaaagcaa atagtccagc attcattgtc
     7261 aatttgccca ttttttcatc ttcataagcg actcctagtt tttctccata aaaatcttca
     7321 tggacataag cagctgctac atggataaat tgtttagcag atcctaggaa ttcatagtta
     7381 tctaagaaca atgccaagaa agaacctgca ttgataccag attgtgggct tgcaccatgt
     7441 gcgcctttac caactaattc gatttttaca tacgtattgt ccgcttcaat cgttccgctt
     7501 actggatttg cttcgatgaa ttggtaaaat gcttcttcca tcgcgatagc tgcatctgca
     7561 cttggtactt gaagtgctgc agttgctgtt cctggcacca tattttcgcg aagtccggaa
     7621 acaaaagatt tcaagacata atctcctgca ttgtcgcctt tgaagcgtaa agctaaagaa
     7681 acatttcctt tttcaccatt gatgattggg aattcagcat ctggtgagaa accaaaatct
     7741 ggttcttctt catgttcgaa ataataagcc atatcgcccc agccgctttc ttcatcgctg
     7801 ccgactacga agcgaacttt ttttgatagt ttcaagtcaa gttctttaat gatcttcatc
     7861 gcatagtaag ctgccatact tggacctttg tcatcgcttg atccgcgtgc aaagatcttt
     7921 ccatccttga ttactggttc ataaggatct gtgtcccagc catctcctgc aggaacgaca
     7981 tccatgtgtc caaatatccc tagcgtttcg tctccttcgc caagatcaat atgccctgca
     8041 tagttatcga cattttttac tttaaatcca tcacgttcgc cataagctaa catatgtttc
     8101 aatgcgtcgc gaggtccagg tccaaatggt gcatctggtg ttacttttga gtcatcgcgc
     8161 tcgctgttga cacgtaaaag atttttcaag tcttcaagca agtcttcttt acgtgcttct
     8221 acttcttttt gccaatcaat tgtcataaag ttaatcctcc acttatttaa gtcttcgtgt
     8281 tcatgatacc acaaattaca gaaaaaaata gttcaaaaaa tctgacaatt ccttttctta
     8341 tctgtttttc tctaaaaaaa atatacgcta taatgagtaa aaggaggttt ttagatgctg
     8401 ataaacgaac aactagtgaa aagagtcatc acacctcgtc caaaaatttc acataaagga
     8461 acatttggac gtatcgtttt gattggcggc aatgaacaat atggcggagc aatcattatg
     8521 agtgctttag ctgccgtaca cagcggagcg ggtctcacga ctgtagtaac tgatgaaaaa
     8581 aatcggagtg ctcttcacag tcatctgcca gaagcaatga ttgtagtttg gacggatact
     8641 gaccagtttc tttccgtaat aaagcaagca gatgtactct tgattggtcc cggattgggt
     8701 acttcttcta agagtttgga acgtttcaaa caagtactag aaaaccaatc aaaagaacaa
     8761 tggctggtga ttgatggctc tgctttaagt ttacttgctg ctaatcatct tttgctaccc
     8821 tatcccgaac aaactgtact tacacctcat caaatggagt ggcaacggtt gagttctcta
     8881 ccgatacaag aacaacggga taaagaaaac aaaaagaagc aacaggaaat cggtgctgtg
     8941 ctcgttttga aaagtcatga gacaaaaatt tatggtcacc agcacttcta caccaatcct
     9001 ttaggttctg ctgctatggc aacgggcggc atgggtgaca ctttagctgg gatgatcaca
     9061 ggatttttag cccagtttcc tcaaaatgac caaacgattg cagcagctat ttatcttcat
     9121 agtttcattg gagaccagct ggcaaaagaa caatatattg ttttgccgac acaaatcagc
     9181 gaagcacttc cgaaatggat gaattatttt tgtaaaaaat caatggaaaa cgaggcatga
     9241 gtcatggaga aatttacagg ttatgtgcga gaaccttttt attatgaaac agatcaaatg
     9301 gggattattc atcacagcaa ttatatccgc tggtttgaag aggctcgggt agccttattg
     9361 gtacatctag ggtttcccta taaaaaaatc gaagaatctg ggatcatcat ccccgttctg
     9421 gaagtaaatt gcagctataa agaaatgatc cattacggag aaaaagtgag catccatcca
     9481 actatccaaa agtatacggg gacacggttg gattttagct acgaaattgt gggaactgaa
     9541 gacaaaaaat taaaaactac tggcagtagt aaacactgtt ttttactttc tgattcgcaa
     9601 cgattagtga agctgagcaa agtcaatccg gagttagatc agttattcca agcgtacgct
     9661 tctttggatg atgtaaattc ttaataaaga ccgtctaagc atttttcttg tattgtgtcg
     9721 agtagttcgt ataatgaaaa gaaaaaagca ggtgatcccc atgtatgtcg ttaaagtaat
     9781 gcacggttac atcgataaaa ctggctgtcg cactcgggaa aaaaacttag acaatctgct
     9841 cattttcaaa gacaaaaaag aatccgaagc ttttgctaaa agaatcggcg gtcgtgtcaa
     9901 accgatccaa gaggtacgac cagattgatt caatattttt cttttcgagg ctctaatagg
     9961 catgcctatc agggcttttt ttgcagactg tgtttttatg cccacacgat ttgtaagcga
    10021 tttatgttat aatagattat atctgcatag aaaggaaggt taaatggtta tcaataattt
    10081 gcaacagcaa gcttatgaat ctatacgtaa acaaatcatt tattccgagt tagcacctgg
    10141 aagtaaaatc tccgataaag tgttagaaga acaactagct atcgggcgta caccgattcg
    10201 agaagcactg atccaactgc gcaatcaaga gcttgtaaaa accattccac agtccggcac
    10261 ttatgtttca aaaatcgata tccgatctgc ttcattagcc cgttatacgc gggaaaagtt
    10321 ggaaaaaccg atcctgcaag aatgcagtgc gaaaatgacc aagcaagatc aagctaaact
    10381 agaaaaaata ctagaacaaa ccgatcaggc agtaatcgct caaaataaaa aagctttttt
    10441 tgatttggat actgattttc accgaacttg ttatgaaatt gctggaaaac gagaaatttg
    10501 ggattggcta gaaagttata gtacgcgttt cagatggtta cgtttgacga tttcagaatt
    10561 agactggggt cgagtattag atgaacatca gacaatgctt caatcaatga ttgatcataa
    10621 ctttgatgaa gtcggatttc tttgcacgat gcatttacat atgattatcg aggagcaaga
    10681 atacgtgatt cataattacc ccgattattt tgaagatatt caggacagac ccatcaaata
    10741 agaggttttg gaatcagcgt tttgacctga gtattagaga aagaaacggc aaaatagttc
    10801 ctcatatttt gccgtttttt ttgagctaat accgagggtc agctgattgc acaccgttta
    10861 ttgagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaatag
    10921 cttcttatgt ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt
    10981 tttccaagag gtcgtgagcc tgccgttcat ctccgagtca caaggaacaa tttcaaaaaa
    11041 cagcttctca tgtttgttga atattttgac ttgtgaccga ggagttgaca ggagaacatc
    11101 gtttattcgg aaaaaaagga ctgtgacacg acttttgtca cagtctcttt atttgtaatc
    11161 taaatcatac tccgtacatc ttttttctat tttttttatt tcttctaaga tcccttggct
    11221 acgaatttca ttatatggcc aaagtaagac tgcctctaat acataaggcg aaaaatcgct
    11281 ttgattcatc tcttttgaaa aactcatcgc tgctatctcg ctcaatgcac gaatgtttga
    11341 ttgatgtgta taaaaaggca accgccacag cacaattttt tcgctttgcg tataaattcc
    11401 cttaacagaa gcttcgatgt ggtgaaatag ttcatgtcca ataatcaaag cttgcgctgt
    11461 ttctttagag atcgaagcta gtcctgaaca ttttaatttt tctaacggct ccgtcatcaa
    11521 tcgaatccct tcatcttctg cataagttgc aaaagtgatt cgttcgttta tttgctctga
    11581 ttcacaggaa acgattggaa cagccatttg ttcacaaatt tctcctacat cggcaaaagg
    11641 gtactttttt ttgatgctac atgccgtttc gataccacaa tcgattgatt gttgtataag
    11701 aattttctgc ttggacaaag gaatcttttt ccaaaataaa tctcgccgaa actggtagaa
    11761 tccccaaatt tcttcgggga tctgttgtaa ttcgtttagt aactcttcta acttatgttt
    11821 gtttgatatt tccggcaacg acgatcactt cttcctccgg ttccatagta gatacttcca
    11881 gatccatcaa aagctgtagt tgttccagat ccgtcgctgt aaaatcagag actcttgctc
    11941 ctaagcagag ataaaattct ggtcgggcaa tcaattcagt ttgataaact gccatatctt
    12001 cccaaagttg ttcgacagct gtcatataat tggctgcagt tgttttcata catgaagcat
    12061 gtccacgttg gaccttgata aatccttttt gatcgatgat ccgaagactt ccagcatttt
    12121 ttgaacgatt ctgttcgccg catacataaa agaaaggtgt attttccaat aagcaaactt
    12181 catttttatt taatcgcata tcctctgcag caagctctag tgcttcttct gttgtgatct
    12241 ccttggttag atcggttgct ttcacttccg tagaaccagt agcgatagcc gttacttttg
    12301 aagtctgtgg atcgatttct acgtggactt cgatcgattc tggtgtagcg cctgattcaa
    12361 tcgctttatt catcgcttca tttttcaacg atcgtatatc ttctttactt ggcgaaggga
    12421 tgatacgttc tacaacatct cggaccattg caagtgctac accaatagaa gaaatcactt
    12481 cggcgttttc aggaatcgaa tatttcacac ccattttatt actaaagtaa gtaatcaatg
    12541 aggcagcgcc gccccctaca cctacaagag aaatctgatc tttctccaag tgatacttgt
    12601 ctgccaattc taaaatcacc ggttcaatct tagcatagga tttttccatg atctgttctg
    12661 cgatgtcttc aaccgtcgta tgacaataat cagctaatgc ttggatggct tttcgtgcag
    12721 atgggacatt tccataagaa aaatgttctt cttgcactaa tccaaggaca ttggctgcac
    12781 atgtatttgt aatagtcact tcttctccgt cttccatcac taccttgacg taatcagcag
    12841 ggtctccttc tttaggagag aaaaattcaa ccttaggacc tttgattttt tctgtctccg
    12901 taaatactgc ataatctaga ccggcaatat gtgcagaacg tggaccgaca tcgatgattc
    12961 ctgactgatt agcccgtacc atcgaaccgc ctgccacacc tagaacccga acatctaatg
    13021 aagagatata tgttgggtgg ccacctacaa ttgaataatc gattgctgga cgtccatttt
    13081 taatgacccc gatattggtt gttgttccgc ctacttcaaa ataaacacca ttagacgctc
    13141 tcaagtacat caaagagccc atgactgatg ctgctggacc ggaaagcatg gttaaaacag
    13201 gtcgtttttt catttcattg atttccatta ctccgccgtc tccgcgcata atcatcaaag
    13261 aaacattgac ccctgcttct cgtacggaat cttctgtaga ggttgccgta tctagcattt
    13321 ttggtaaaat agatgcattg attgctgctg tccgagttct tcttgtcaat ccatataatt
    13381 ttgtgatgtc agaagccatc gttgtaggga tcgattttac cgatgctgct tcataaacaa
    13441 ctcgttctgg ttccccatta tctactccaa atgccataga agaaaccaag acctccgctc
    13501 tttctctttc taaagaagaa atcgtttctg aaacacggtc tacatttaag tgtttgacag
    13561 gtaagaatgc attgacaatc tctatttttt tcttattccc taaatcgata tcgttcaatc
    13621 tagtttgtcg ttttgctaaa aaaccttcta atccaccttt tgccatcccg attacgccga
    13681 cttttgctac gtctccttca atcagtgcat tagtcgcttg tgtggtagaa tgtgcaacaa
    13741 acacaacatc ttctggactg atattatttt ccagcaaaca attttgaaaa gactgaacga
    13801 cacctgcggc tactccacgg acatcatcgt gggttgtttt cactgaagat tttccgatga
    13861 tttcgtgcgt cgcattatcg attgcgactg ctttcgtatg tgtaccgcca acatcaattc
    13921 ccattcgcac aaaacgtttc attttctttt cctccattca tctaatcgtt tttatcgacg
    13981 ttttgtttac taggcgttcc cgtacaataa gaacgtgatc aggcataaga tcgctccgat
    14041 gatccaacca gttgggatgg atttcttcat atattctcga gaagttactt ttgtataacc
    14101 taatccccaa gcaacccaag attgtgtaat atctaaatgc tgaggagcaa tcgttgtgat
    14161 tgcaaataac gggaacaaaa aggctggcga catcgttgga ttaacagcta atacgacagc
    14221 taaaatagct gagcctgaac cgactagatt cattggaccg cggaaaaatc ctagtggtgt
    14281 caatatagca aatacgattg ccaagactaa tggtgattgc ggtatccaat ctccaaaaat
    14341 agctgaaaaa taaggagaag catacgcagc tgaattattg aacatcgcta aggtcatcaa
    14401 gaaaccaacc attggcgcaa catctactgc gccatctgta aataatttcg caaactgacg
    14461 acagatttct ttataacctc cgcgcaattt tccagtaaac agcaatgccc agatacctgc
    14521 tagaataaaa cctaagatga ttggaagatc taaaagaacg acccctaaaa ctggtaataa
    14581 tacggctaac catgagatca ttggtgcgtc gccaccttca gctggtacgt ttgctgccat
    14641 tgcatagcgt ttctttttgt tcattgatat attagcgacc gttagtacca caactaagct
    14701 tacaatcatc ccaatcatac caatttggaa atagtcatta taagtatagc tttcagccgc
    14761 tggattgaaa ccagcataaa tcgtttgata ttgtttgaaa ttgacgatat tagcaaagat
    14821 tccagccata atcgagccca taaatgagaa cagtgcaacc ggtactgaaa tacctaatga
    14881 catcaagatt ggcaacacaa taactgcaat agaaatcact ggtccgattc ctgtcatcga
    14941 catgaatata acagcggtta caatacatag taagctcatc gtgattcttg gcttgtcccc
    15001 tccaagttca actacctttc gaatcagggt agctgcgatt ccgctatcta ctagaacacg
    15061 cccgaaaaag gcaccaaaga agacatttac caagatcgat ttggcgtact cagctggccc
    15121 cgtcgtatag atatgcgtca acacatcaat tactccttgc ccttccatcg ccgggtttgg
    15181 tgaaaacgtg tttccgacta gcgctaatcc catccataca gtagccatga tggcaaagcc
    15241 gaccatcaag ttgtagcctt ttacacagta ccagaccatg aaaaagaatg agataactaa
    15301 caaaatacca ataataatat cactcatgtt attcctccgt ccattttgtt tttctgataa
    15361 gcaaactaat atatcagtca aactcattct aacttatgta gttagcgctt tcattaaagg
    15421 gtgaccataa atgttcagtt ttcaattaaa aaaccttaat tatgctattt tagacttata
    15481 cgttattgtc ctgtttcatt tgatggagga ttgtaaataa aaaaagagga aagaacataa
    15541 cttttcgtta tcttctttcc tctttataat tgtttagctt atttagccaa agcttctttt
    15601 gcttggtcag ccaatgcagt aaatgctgct gcatcgttaa cagccaagtc agctaacatt
    15661 ttacggttga tgtcgatttc agcaactttc aaaccgtgca ttaattttga gtagctcaag
    15721 ccattcatac gagctgctgc gttgatacga gcaatccaca atttacggaa gtcgcgtttc
    15781 ttttgacgac gatcgcgata tgcgtagttg tatgaattca ttacttgttc ttttgctgtt
    15841 ttaaataaag tgtgttttga accgtagtaa ccttttgcta attttagcac tcttttacga
    15901 cgtttacgag ttactgttcc acctttaaca cgtgccatgt ataattcctc ctaataaaaa
    15961 taaatcttca gattctgtta tttgtacttc ttatttcatt tttgctagtt gttggcgaat
    16021 acgtttgaag tcgccactag aaaccatgct tgctttacgc aattgacggc gttgtttttt
    16081 tgttttgccg tggaaacggt gacttgtaaa cgcgcggaag cgtttcaatc cgcctttacc
    16141 agtacgtttg aaacgttttg ctgatccgcg gtgtgttttt tgttttggca tgactatatt
    16201 tcctcctcaa aatctttcgt tatccgacta tcggctgtcc aacagccagt tagtcattca
    16261 aattacttac tgtcgttttt cggtgccagc gtcaagaaca tgctgcgtcc gtccattttc
    16321 gctttttgtt ccactgttgc aatatccgca gtttcttcag ctaagcgatt caagactttt
    16381 tgaccaattt ctttatgggt aatggcacgg cctttgaaac ggatagaagc tttcacttta
    16441 tcgccctttt ccaagaactt acgtgcatta cgaagttttg tattgaagtc attcaagtcg
    16501 atcgtaggac ttaagcgaac ttcttttaca ttgatcactt tttgtttttt acgcgcttcg
    16561 cggtctttct tttgttgttc gaaacggtat ttaccgtagt ccatgattcg cgcaactggt
    16621 ggtttcgcac caggggcaac taacacaaga tccaagtttg cttgttctgc aatattcaat
    16681 gcttctgctt ttgtcttaac tcctaattgc tcaccatctg aaccgatcaa tcgtaactct
    16741 cgtgcacgaa tgccgtcgtt aaccatcata tcctttgcta tggtcattca cctccaaata
    16801 ttatgagaga aagaaaagct taccgaataa acggcaataa aaaaacgagt tctcttacag
    16861 aacccgcgcg tacttcacct aatagaacca gtgaaaacta tctagcccag tgacattatc
    16921 aactaaggcg agaagcgggt gcttctgctt ttatttctca accatcatag tatagcaaaa
    16981 actaatcttt ttgtcaatgt ttttcataca gtttgtcttc cttttctgaa aatgattaga
    17041 ttagcagact ttctagcagt gattcccgct tctttgctat actaaagaaa aacgaggtga
    17101 acccacatga aaaaaatcga acctattcca ccttctcttc ctcttcttac aaagggagag
    17161 ttttatttag aagatgagga gatcttctct ggtcttctga tcaacggggg tgaccaaagc
    17221 tatctcgatg ccaaaaatat ggtattaaga gagagtcata tccaaaaact tacgatgcaa
    17281 aaaacagttt tacgtcattt tgaatgcagt aatgtcattt ttgaaaaatg tgatttttcc
    17341 aatctggagt ggattggtgg cagtttccac caagtcgtgt ttcatcaatg taaattaaca
    17401 ggtacgaatt ttgccgaaag ttatcttcga gactgtactt tcactgattg tattgcttcg
    17461 atggcttcct ttagtagtac aaacttgaaa gctgtctcct tcgatcattg tcaattagaa
    17521 gacagcgaat tttatgaggt cacttggaaa aacctttttt tgtctgacaa tcagttgacc
    17581 ggttcgaact ggtttcgaac ttctctgaag ggattagact ttacgactaa tttcttcaca
    17641 gcaatcgctt tatctcaaga ttctttggcg ggactaatcg tcaaccaaga gcaagcactt
    17701 gtgatcgccc aagcccttgg tttgcagatc aaagactaga tcaacgaatc cgccgccaca
    17761 gtgcaataag catccaaata gataagacca aactgatgat caaggcgatt ccccatccga
    17821 aaacggaatg ggtaaagggt aactggagat tcattccgaa gaatcctgtc acaatagttg
    17881 gaacggtcaa gatcagcgac cagaccgtca aaaatttcat cgtgtcattc aagttgttat
    17941 tcagcaaatt attataagtg ccagagagct ggtctaaaat ctgcgaagct aagttcgtca
    18001 tttcaactgc ttgtcgtgct tcgatcagcg catcgtccaa ctcttctttt tccttttctg
    18061 acaacttgcg ataaatagcc aatgccttga tttgttctaa aagtactaca ttttgttttg
    18121 ttcctgtaac taagtaaacc aaaccgatct ctaaatctga aagttgcagc agattcttgt
    18181 tcgttgtttt ttctcttaat ttcagattaa gccgctgacg ttcactattc acttcctcga
    18241 ttaaaggaaa aaaggaatca gaaatcaaaa acaatgtacg aaacaataaa ctgtatacac
    18301 tcatgtctgg cattcgttcc aaaagcgatc gaattagtcg aatcacgtac tcggtattcg
    18361 gcgtggtaaa tgtaaacaaa ccgtcttctt ttaaaataaa agcgatagga ctagtttcat
    18421 aatgattatc gactttttgc tggtgcggaa catttgatac aagcagtaac gcttcatcaa
    18481 aagaatcgta ctctacgcgc gcccgttcat ttttatctag agaataagtc agcatctcgt
    18541 cactaatgcc atattcttct tttaagtttt taacagctag cacatcgttt tgctgtacat
    18601 tgatccaaaa attacggtct tgattaaaat agtgtttttc ccgcatactc tatcctcctg
    18661 acatttgttt ccagtataaa gaaaaaagtc gctcaaataa aggtttaccc tttatttgag
    18721 caactgtaaa tcaaatggct actttggttt ggcgaatctc tgctaatttt tcttcgatca
    18781 ttttcaagac ttcttctttt gcttgaggat cttccacaaa attatagcgg tctccatcga
    18841 tttggatttt tggactttct ttgtattctt cataccattg atcgtaacgt ctgttcaatt
    18901 ctttgtaata atcgtataga cttggatcaa aggacaattg ttcatacgaa cgaccacgtt
    18961 tttcgatacg ctccaacatc gtatcaaaag atacacgaat atgaactaat aaatccggat
    19021 gttttttatg ggcagcatag ggaagttctt ccatcatgtt ttccagcagt tcatcataca
    19081 cacgaacttc tgtttctgtt gctcttccta gatctgcatt tagatggaaa agcagtgaat
    19141 cttcataaat cgaacggtcc aatacattat tgtcatcttt cattgcttgt ttgatgcttg
    19201 caaaacgttt attcaaaaaa taaatttgca gcaaaaaagc atactgttca gggtttgcat
    19261 aaaataaggg caagacttca ttatcatcaa tcgattcata aaatgcctga ctatcaaagt
    19321 gttcagccat catttctgtt aaacttgatt ttccggcacc aattgtacct gctaaaacga
    19381 tcacgctcat ttacctccag ctcacttcat cactaaatat tgtgtttaac tccaaccttt
    19441 ttaaatatac ccccaatatc tagtagtgtc aattgtattt ttttctagtc aaagaagaaa
    19501 aaaagtcaaa aaattcttaa gaaaaaagtg ttttttcttt agaaaacgta ttcttagttt
    19561 tacttctgag atggttttag taaaataaag gaggaatgtt ttttgagacc cttagtggac
    19621 aaaaaaattc gtgaagcagc gcaactcgtt ttgggcgctt tattttttgc ttaaaaatca
    19681 cttttacgtg agaaggagtt agattatggc aaactatggc caaggagaat caagcggaaa
    19741 gatcatattg atgggcgagc acgcggttgt ttatggagaa ccagcgattg cctttccttt
    19801 ctatgcaaca aaagtcaccg cattccttga agagctggat gcaatggacg atcaactggt
    19861 ttcttcctac tattcaggaa atttagccga agctcctcat gcattaaaaa atatcaaaaa
    19921 attattcatt cacttaaaaa aacagcatga catccaaaaa aacttgcaac tgaccattga
    19981 aagcacgatt cctgctgaac gtggaatggg atcaagcgct gcagtcgcca cagcagtcac
    20041 tcgtgctttt tatgattact tagcatttcc tttgtctcgt gaaatactat tagaaaatgt
    20101 ccagctttcg gaaaaaatcg cccatggtaa tcctagtgga atcgatgcag ccgctactag
    20161 cagcttgcag ccgatttatt ttacaaaagg gcatcctttc gactactttt ctttgaacat
    20221 cgatgctttt ttgattgtcg ctgatacagg aatcaaagga caaacaagag aagccgtcaa
    20281 agatgtcgct cacctctttg aaacccagcc tcatgaaact ggacaaatga tccaaaaatt
    20341 aggatacttg acgaagcaag caaaacaagc aatcattgaa aattcaccag aaacgttggc
    20401 acagacaatg gatgaatcac aatcacttct ggaaaagctg acaatcagca atgattttct
    20461 taatctattg atccaaacag caaaagatac cggggcattg ggtgccaaat taactggcgg
    20521 tggacgcggt ggctgtatga ttgcattagc acaaacaaaa acaaaagccc aagaaatcag
    20581 tcaagcactt gaagatgcgg gtgctgctga aacttggata caaggattag gagtacatac
    20641 ctatgtttaa aggcaaagca cgcgcatata ccaacattgc tctaatcaaa tattggggta
    20701 agaaaaatga agaactcatc cttccaatga acaacagcct ttcgttaaca ttggatgcat
    20761 tttatacaga gacagaagtc atcttttctg atagctatat ggtagatgaa ttttatctag
    20821 atggcacctt gcaagacgaa aaagcaacaa aaaaagtcag tcagtttctt gacctttttc
    20881 gtaaagaagc cggcctctca ctaaaagctt cagtaatcag tcagaatttc gttcctaccg
    20941 cagctggatt agcctcctct gccagcgggc tagctgcttt agcaggcgct tgcaatactg
    21001 ctcttaagct tggattagat gatctctctc tttcaagatt tgctcgacgc gggtctggtt
    21061 cagcttgccg aagtattttc ggtggtttcg tcgaatggga aaaaggccat gacgacttaa
    21121 gttcttacgc taagccagtc ccttccgatt ctttcgaaga cgatttagca atggttttcg
    21181 ttttgatcaa cgaccagaaa aaagaagtgt ccagcagaaa tgggatgcgt cggacagtcg
    21241 aaacatccag tttttatcaa ggctggttag attccgttga aggggatcta tatcaattga
    21301 aacaagcaat caaaacaaaa gatttccaac ttctcggaga aacgatggaa agaaacggac
    21361 taaaaatgca tggaacgact ttagctgccc agcctccatt cacttactgg tctcctgaca
    21421 gcttaaaagc gatggatgcg gtgcgacaat tgagaaagca aggcattcca tgctacttta
    21481 cgatggatgc tggccctaat gtcaaagttt tagtggaaaa cagccattta tctgaggtac
    21541 aagaaacatt taccaaatta tttagcaaag aacaagtaat tactgcacat gctggtccag
    21601 gaattgcgat tattgaataa agggagattt ttatgattga agtatctgca ccaggcaaac
    21661 tctatattgc cggagaatat gcagttgttg aaacaggcca tccagcagtt atcgctgcag
    21721 tcgatcaatt cgtgacagtc actgtagaat ctgcacgaaa agtcggaagt atccaatctg
    21781 cacaatatag tgggatgcct gtacgttgga caagacgcaa cggagaatta gttttagata
    21841 ttcgggaaaa tccttttcat tatatccttg ctgctattcg cttgactgaa aagtatgcac
    21901 aagaaaaaaa cattctttta tccttttatg atctgaaagt aacaagtgag ttagacagtt
    21961 caaatggccg gaaatatgga ttgggttcta gcggtgccgt gaccgttgca acggtcaaag
    22021 ccttgaatgt tttttacgcg ttgaatttat ctcagttgga gattttcaag attgcagcac
    22081 tagccaattt agcagttcaa gataatggtt cctgcggcga catcgctgct agctgttatg
    22141 gtggctggat cgctttctca accttcgacc atccttggct ccaagaacaa gaaactcagc
    22201 attctatcag tgagttactt gccctggatt ggccaggtct atccattgag ccattgattg
    22261 ctcctgaaga tttacgttta ttgattggtt ggacgggtag ccctgcctct acttctgatt
    22321 tggtcgatca agttcaccgt tcgagagaag ataaaatggt ggcttatcag cttttcttaa
    22381 aaaatagtac agaatgtgtc aacgaaatga tcaaagggtt taaagaaaat aatgtaacgt
    22441 tgatccaaca gatgattcga aaaaaccgac aattactgca tgatttatct gcaatcactg
    22501 gggtcgtcat cgaaacgcct gctttgaaca aattgtgtaa tttagctgaa cagtatgaag
    22561 gagccgcaaa atcttctggt gcaggtgggg gcgattgcgg aatcgtaatt gttgaccaga
    22621 aatctggcat tcttccttta atgagtgcat gggaaaaagc agaaatcact ccactgccgt
    22681 tacatgtcta tagcgatcaa agaaaggaaa accgatgaat cgaaaagacg aacatgtttc
    22741 actagctaaa gcctttcatg ataaacaaaa aaacgaattt gacttcgtac ggatcatcca
    22801 taaccctttg ccgcaaatag cagtttctga tgtcgatcta agtacacagg cagttgggtt
    22861 tacactaagc agcccatttt atatcaatgc gatgactggc ggaagcgaaa aaacaaaaaa
    22921 aatcaaccaa gatcttgcaa tcgtcgcaag agaagcagat ttgatgattg ccactggttc
    22981 agtcagcgct gccttgaaag tcccttcttt agccgatact tatacgatta tgcgacaaga
    23041 atatcctcac gggaaaatca ttgccaacat cggtgcaggg acttctgtcg aaagagccaa
    23101 agaagctatc cgattgtttc acgcagatgc cttacagatt catttgaatg cacctcaaga
    23161 gttggttatg cctgagggag accgtgattt taccaattgg aaagtactta ttcaagaaac
    23221 tcaaacagcc attgacgtcc ccctcatcgt aaaagaagtc ggttttggca tgacaagaga
    23281 aacgctcaat gatctagctg ctttaggagt tcatacagtt gatatcagcg gtcgaagcgg
    23341 aacaagcttc acccagatcg aaaacgcccg tcgctccaag cgggaactga gttatttagc
    23401 tgactggggg caatcgacag tctcttcatt gcttgaagca aatgaagcag acacttctat
    23461 ggaaatccta gcctccggag gtatccgcaa tgcctatgac atatttaaag ccctttgtct
    23521 cggcgcaaat gccgtgggta cttcaggaac cgtactgacg catctgatga atcacggcgt
    23581 agaagaaact attatactga tgaagcaatg gcaagaagag cttcgtctgc tttatactat
    23641 ggtcggcgca acgaataccg cggccctaca ccaacaatct ctgatctttt ccggaccagt
    23701 gaaggattgg tgcgaagcaa gagggatcga cttagtgaag tatggaagac gcacagaaaa
    23761 gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt acaaacggcg
    23821 gaagtaaaag caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt
    23881 tcgtgaattc cttcttattt cttggagtta aatgcttctg tcccagcctc ttttttcgaa
    23941 taaatgatgt ttaatcagct gtccttcggt attcgttcaa tccgctgatt tcacaacctc
    24001 tccactaaac agtgtactaa agctctttta aggttgtttt ccgatatagg caagtatgcc
    24061 tccgtccaca tacaagacat ggccgttaac gaaatcagat gcgctagaag ccaagaagac
    24121 agatggtcca gccaaatcga ttggctcgcc ccagcgtgct gctggcgtac gtccaacgat
    24181 aaattgatcg aatggatgac gttcaccgtt ttcttgtgtt tcgcgtaatg gagcagtttg
    24241 cggtgtagca atataacctg gtccaattcc attacattgg atattgtatt gaccgtattc
    24301 agaagcgatg tttttcgtca gcatttttaa tccgcctttt gccgcagcat aagcactgac
    24361 cgtttcgcgt cctaattcac tcatcatgga gcagatattg atgattttac cgccgccttt
    24421 ttcgatcata tccggaataa ctgcttttga aacgatgaat ggggcattta aatccacgtc
    24481 gattacttgg cggaagtctg ccgcatccat ttcgatcatc ggtgtccgtt tgatgatccc
    24541 tgcattgttg actagaatat caactgaacc tacatcttct ttgatctgtt gaaccatcgc
    24601 ttgaaccgct tcttcatcgg tcacatcgca gacataacct tttgcatcga tgcctgcttc
    24661 tttataatgc gctatacctt catcaactga ttcttgattc aagttattaa atacgatttt
    24721 tgctcctgct gtagctaaag agcgtgcaat ctcaaatcca ataccataga cagcaccggt
    24781 gactaatgct acttttccat ctaaacgaaa catatccata ctaaattcca tcattttctc
    24841 tcccttatgc ttattttaat tgatccattt gcaccatatc catgtctgta taggtgatat
    24901 tttctccgca cattgcccag atgaatgagt aattgcttgt acctacacct gaatggatcg
    24961 accagcttgg cgaaataatg gcttgttcat tgtctacaac taaatgtttt gtttcatctg
    25021 gtttgcccat catgtggaac acacgtgtat cttcattggc ataatcaaag tagacataag
    25081 cttccatacg acgttcatgt gtatgacaag gcatcgtatt ccaagcgctt cctggttcta
    25141 aaatcgtata acccatttgt aattgacagc tttcgcatac atttggatgg atgtattggt
    25201 agattttacg ttcattcagt gtcaggcctt caccagtctc cattggtttt acttggtcga
    25261 tactgatttt tacatttgga tatttatgat gagctggaac agaactaata tagaatttag
    25321 ctgggttttc agcatcttga gaagaaaata caacatgtcg tgtttctttt ccgatatagt
    25381 atccgtcttg ttttttcatt tcttcttttt gcccatcgat ctcgatgaat cctggtccac
    25441 cgatattgat cacacctagt tcacgacgct ctaagaagta atcaacacct agttctttgt
    25501 ctaatgtgat ttctaattct ttttcagtag gtgtcactcc accaaagatc attcgatcgt
    25561 tgtgcgtata agtcaaactg attttccctg gttcaaatac cttttctact aaaaattctt
    25621 ttctcaattg ttccgttgaa taatgacgga tatcttctgg actgtgtgta taacgtgttt
    25681 ccatttcttg cattttcttc tactccttct atcgaatgat ttttccagag ccgcatgcta
    25741 aaaattgtgc gacttcttct tgactaaatt ggttgcagtc cccatggatc gtatgcttca
    25801 aggcagaagc tgccgtagca aaatcaatga tttcttgcgg agacatattg gataaaatcc
    25861 catggagcac gccacctgca aaagcatctc cgccacctac tcgatccacg attggggtga
    25921 ttctatggac ttgggattcc acatattcat ggttcatcca caatgtcccc ttcaattcat
    25981 tttcacttgc tgaatgaacg gtacgcttgg tcgaataaaa cacctgaata ttcgggaact
    26041 tttcctgcat tttttgataa taataaatta gaggttgttc ttctgcttct ggcgctttct
    26101 cgatacctaa caaataaaga gcatccattt ttccagctga gcagatatct actaaaggta
    26161 aaagctgatg gattacttcg ctggcttctt gctgtgtcca taattttcca cgatagttga
    26221 tatcgaagct gatcaggcag ccagcttttt ttgcagcttc tatcacctgt ttcgtcattt
    26281 tttgccattt tttagacaaa gctggggtga ttcctgaaac atgaaatata tctattcctt
    26341 ggaataactc ctctagcgac cattccaaat gatccatttc agcaaaactt gatccggcac
    26401 gatcatagat cactgaggcg gcgcgttcgc cactaccagt ttccatataa taagtaccta
    26461 gtcttgatcc accagataat acctgtttcg tatctactcc ataacattgc aagtgctttt
    26521 tgactgcttt tcccaatgcg ttatcaggta cttttgtagc aaaaatcgct tcatgtccgt
    26581 aattagctaa agaaattgct acgtttgctt cccctccgcc ataatgggca gcgaatcgtt
    26641 cacttgaatc gatacgggtt ccctgctctg tggacaatcg caacatgatt tctccgagtg
    26701 tcactacttt tcccataagc agctacctct tgatttcttt catttttgct gcatactgtt
    26761 gcgcgacttc cgttactttg tcaaaatctc ctgtagctgc gggtgctaaa aggttcccac
    26821 ctactccgac tgttacgaca ccagcatcga accactcttt catattgtcc agactcacac
    26881 cgccagttgg catgatattg acttgaggca tcggcgcctt gaaggcagaa atgatacttg
    26941 gtccataagc gcttccagga aatagtttga cgatatctac cccgcttttc agcgctgttt
    27001 tgatctcggt aatagtcata catcctggta agtaaggaat ttgatacaga ttgcacatct
    27061 ctgccgtttc ttgatcaaag gaagggctaa caatatactc tgcgccagct aagatagcca
    27121 agcgagctgt gacagcgtct aatactgtgc ctgcgccaat cactatttct ggttggtctt
    27181 tgtagaatga caaaagttct ttgattactt gatctgcttg agggactgta aaagtcaact
    27241 caataccagt tagaccacct ttgatgattg cgtggcaagc attcaaagct tcttctttgc
    27301 tttttccgcg taccaccgca atcactccgg cgtttttcaa tcgagataaa atatcaattt
    27361 ttttcatctt ctccacctat ttcctattta gaaattttat tttataactt ggaaatctat
    27421 acccatatta tacggcagaa aagaaaaatt gcaaacgctt ttttcattat ttttgaattc
    27481 tttgctcgat ttcgcttttt gtttgcttga gggcactgat tatttgttct tttgtctcat
    27541 ctgttaaacg atatttcggc atacttacac taaatgcacc aatgatttgt tcgtttttca
    27601 cgattgaggc tcctatacaa aaaatatcac gttcgatttc ttcatcgtca aatgcgatgt
    27661 gggtttcttt taccgttttg atttcctttt tcaaccgtaa tgcattggtg atcgtatatt
    27721 ctgtgtaagg gatcaacgga tgcttttcaa gataatcagt gtactgctct tcactgaatt
    27781 ctgctaatac ggctttgccc atcgcagaat tgtataatgg tcgggtgatt ccgatttttg
    27841 aagacatgcg gatcgtttga tgttttggat ctagtttgtt cacgtacaag atctcattgt
    27901 tgtccaatac ccctaagtgg atagtctcat cgatcttttg ctgcagctgt tctaaaaacg
    27961 gtaaagtcaa ctctaccaaa tcgatttgtt caatgttttt attcgcgtag cgaataagct
    28021 ttgcacctaa acgatagttt ttctctgaat ttttatttac gtagccgatc atcaacaaag
    28081 tatctagtat tttcaagact gtagaagagg tcattcccac accctttgcg atcgtctgca
    28141 aagagacatc ttggttttcc gctaaatagt ctagtatttt agaggctttg atcaatacag
    28201 ttccataagg ctttttattt tccatataat attctcctat ttctttataa gaaaaattat
    28261 agcggaaata ccatcaattt aaaagagata tccagatata aactaccatt tctctttaca
    28321 ggcaatttaa tgttgaagat ccgcttcgtt tttacatcaa actttgatat aaagcaatga
    28381 tttcttctac tgtcgtttct cttggatttc ctggtgtgca tacatctcgc aaagcatctt
    28441 ctgcaattgc tggaatatct gcttctttta cacctaattc agaaatggtt tctgggattc
    28501 caaccgcttt gctcaaccga tcgatctcac cacaagccgc ttctcttaca tcttctaaag
    28561 acatttcagc tgctccttta atccctagaa caagagcgat ctcacggtac ttttctccag
    28621 tatattcttt gttatatttc atgacagttg gcaaaagtgc tgcacacgct acgccatgag
    28681 gaatgttgta ccatgcactt aacgggtgag ccatcccgtg taccagtcct aaaccgacat
    28741 ttgagaagcc cattcccgca atatactgtc ctaatgccat tttttctcgc ccattttgat
    28801 cgccatctac agaagcttcc aaagaatggc cgatgatttc gattgctttt aaatgcagca
    28861 tatcagtcat ttcccaagcg ccttttgtga taaacccttc aatagcatgc gtcatagcat
    28921 ccatacctgt tgatgcagcc aattttttcg gcattcccat catcatatcg ctgtcgatga
    28981 acgcgacgat tgggatatca tgaggatcaa cacaaacaaa ttttcggtga tttacttcat
    29041 ctgtgatgac gtagttgatc gttacttctg ctgctgttcc agaagtcgtt ggtatagcta
    29101 atataggtaa acatggattt ttcgtatcag caacaccttc taaactaatg acgtctgaaa
    29161 actctggatt cgtcaaaata atcccaatgg cttttgccgt atcgattggc gaacctccac
    29221 cgattgcgat caaacagtct gcctctgcct ctttaacaaa agccactcca tcttttacat
    29281 tttggatcgt tggattaggt acgatcccgt cgtataaagc ataagcgatt gctgctttat
    29341 ctagaagttc tgttactttt gttgctatac cgtgctcgat caatcctttg tcagtaatga
    29401 cagctgcttt gccgaatcct cgctgctgaa attcagggac gatcgactca atcgctcctt
    29461 ttccaaaata cgatgtttca tttaaaatca ttcgattact cattttctat tcctcctaat
    29521 ttgtattatt tttcacaata aaaacgaaag aaccccaagc aaccggttgc ccagttgctt
    29581 gttgctacag ttcaaagact ggcatcaatt cccaactgac tggagaggca tctggatttg
    29641 tctccatgat atcttccatg tacttccacc atttttgatt gatagatgta tttgccattt
    29701 tttcccataa cttttcatct tcgatcacta agtaaccaaa taacaaactt gtactcggat
    29761 caagatagat cgaatagtcg atgaccccgt gttcacgaag tgctgtacgc atttctggcc
    29821 aaagttcatc atgtcttttt ttgtattctt cttgtttttc tgggtaaact ttcatgcaaa
    29881 aagctttttt caccatgtca gacacctgct ttcgtatgaa cttcctccaa ataacctgct
    29941 tttggagtga caccaaacgc atctgctaaa tgccacaggt tttcgtccgt gattgtttgc
    30001 aagattggtc cttgcgcttt gacgtacgta tagacttctg ctgccttttc tgctgtctcg
    30061 atcaaaccaa agacttcatc catgtctttt cctgacccgt agattccatg ttgtggccat
    30121 aaaaccaatc gagtttcttt cattttttca gctgttgctt ccccaatctc atttgttccc
    30181 ggaaccatcc acggaatgat ggaaattcct tctggaaaaa caactaagca ttccgtacac
    30241 atttgccata gcgtacgact aaaggaacgt tcgtctaatt catgcgtaaa actcatcgct
    30301 aatagatgac tagcatggca atgcataacg atccgattat ctgggtctac ttctaaacgg
    30361 gcgatatgac tcatgaaatg acttggcaac tcgcttgtag gaaggcattc gttttccagc
    30421 ccccacaaaa gttccagtgt tttgccatct tcagcaactc gaacgattcc aagattctcg
    30481 gcaggttcgc tcgctacatt tttaaaatat cttcctgaac cagttacaat aaaatagcat
    30541 cctgctaaac ggctggcatc aaaaatcatc gagatgcggc ggatcacttt ttcaggatcc
    30601 aagaacggtg tgatctcttc ttcttttagt aaataagaaa tattcccgcc atttctttca
    30661 tcccatccca agcgataaag attcgacgtc gtttcgatca tttctttcac ataaggcgct
    30721 tgtaaaatat ctattttttt caatatagcc acactcctat tttcttaatt ccaacacgtc
    30781 tttttgatat tcctggactt cttccagcca atccaaacca actggaactt gatttttgta
    30841 acagaaataa ttccatacat cggcatacgg gaaatctttc aattcttccg tataagccat
    30901 tcttgaagta aagtcaaaag ccagctccat ttctttcaat cgctcaactg gttccagcat
    30961 agctttcagc agtgcttttt gtgtatttct cgtaccgatg acccaagcag cgatacggtt
    31021 gatcgttgca tcaaagaaat ccagaccgat atttgttttt tccaacaatt cgttacggac
    31081 taattcttta gcgatttcct gcaattcatc atccatcaaa accacatggt ctgaatccca
    31141 acgcactgga cgggaaacat gcaacatgat tcctttgctg aataaagcta atgaagataa
    31201 tttattggaa atcacttctg ttggatgaaa atgtccagca tctagacaaa caagtttgtc
    31261 acgagtcaac ccgtaaccca tataaaattc atgggaaccg actgtataag cttcagcgcc
    31321 taaaccaaat agtttgcttt ctactgcatc ttgtacatac cgttcatcta actcttctgt
    31381 aaaaatttca tcgagtgatt ccattaaacg ttcccgtggg gtaaaacgat caatcggatt
    31441 atctttcatc ccatcaggta cccaaaagtt attgatactt gtttgcccag tttctttccc
    31501 aaagtactcc gcgattttgc ggcttctttt cccgtgttcg atccagtatt cacgaacttc
    31561 tttatctgga tgagccaatg taaagccgtc tttcatcatt ggatgcgaaa agaaagtcgg
    31621 attgaagtca agaccgattt cttctttttt tgcccactct acccattttt caaaatgttt
    31681 cggctcaatt tcatttagat cgacagattc ttctgtatcc aaatagatag catgaaggtt
    31741 caatttgtgt ttgcccggta tcaacgaata agccttctcc agatcttgtc taagctcttc
    31801 aggtgttcgt gcggctcctg gaaaattccc tgtcgccatg attccaccgg tcaattcgcc
    31861 atccggtgtc aagaaacctt tgacatcatc tccttgccag caatgcatag aaattttgat
    31921 gtcctgtatc ttttctagta cagcatctgt atctacaccg atagttgcat atttttcttt
    31981 tgcttgttcg taatttcttt caatcgtgtt catacgatta ctcctttgat tttattcacg
    32041 taccattcca tcgtgttttg cttattttca tctgggtaaa aagtttcaca tggaaacgac
    32101 tgtttgatta catttcttgc ttgttctata ttcgaaaact gacctgtact gagcatttgc
    32161 atggcaacat tcccgattgc tgtggcttca ctaggtcctg cttctacaat gattccggca
    32221 taatctgctg tcaattgatt cagtagacga ttattcgatc cgccccctac gatgtgcagc
    32281 ttttccaatt tcgttccagt taagtgttct actttgatca gctcttgtgc gtaacaaaga
    32341 gcaagattat catacacaca acgagcgata gctcccgatg agacagggac tggctgctct
    32401 gtttcaaaac agtattgctg aatctcttct atcatatttg ggggattcaa aaatcgaggg
    32461 tcattgattt ccatcacttg ctgcagcggt ttttcttttg cagcaagctc tgccaattcc
    32521 gcataactgt actcgtactc ttgcattcgg gcaacttcct ggatcaacca catccccatg
    32581 atgtttttca aaaaacgcac cgtgttatgg gcaccccatt cattcgtata attttcagaa
    32641 aaagcattgc gagaaacatt ggcgatcttt gtttcaatcc ctaaaagcga ccatgtccca
    32701 ctgctgatat aaccccaacg ttctccttga cctggcgttc ctaatacagc tgacgccgta
    32761 tcatgacttg ctactgtgat aaaggtcaca tctggcaaat catattttga aaattgttct
    32821 ttttgcagat ctcctagtat cgttcccggc tctgtcaatg gtggaaataa ttccttcgag
    32881 acaccgattt tttcaagaag ttctatttcc cattcttttg tccctgcatt cagcagctgc
    32941 atagtagatg cattagtttt ctccgtcacc atgttaccag taaagacata ccctaaataa
    33001 tcaggtatca gtaacaattt atgtgctttt gccaaatttt ctttttcttc agcaaacaat
    33061 tggaacaagg tattgaaagg ctggatctga atccctgtct gctcatacaa cttatctaga
    33121 gaatagattt cagaaaactg ttctactgct ccattggtac gcttgtcccg ataagcaatc
    33181 ggatcattta tccgatcccc ttgttcatca atcagacagt aatcgactgc ccatgtatcg
    33241 atccccgcaa aaaggcgatc aatccctgtc tgctttaatt tttcaaatcc aatcaaaatc
    33301 tctttgataa gataatcgat cttccagcga tcgtgaccgt ctttgttccg aaagccgttt
    33361 ttgaaccggt gtacttcttc caagatcaaa gaaccttttg catccctcgt acttttcatt
    33421 agtcgtccgc ttgatgcacc gatatcgatg gcaacaaatg ttggattcat ttctccacct
    33481 cttttacttt attgttgatt agcactttat taataaagcg ttatcattta ttgacaatgc
    33541 tatcataata cagaatctta tattttgctt cacactattt tggtatataa ttgcattatc
    33601 ttgacaagtt gggagtgttt acatggatta ctgggaaaaa gccaaccact catggtcgga
    33661 agactcaaaa agagtgatct tgacgcctac acaaagaagc aggaatttat ttttttatat
    33721 ccaagaaatc ggttacttca aagcaccaat cccttactat actgaacgag cacatttacc
    33781 ttcttacttg ataaagttta ctttgtctgg caaaggttcg cttacatata atgataatca
    33841 gcaacagcta aaagctggag acttattttt tattgactgc caagaatatc agcgttatca
    33901 aacgatcagc gaagaaccat gggaaatgga ttggatccat ttgaatggtc agaacgtcca
    33961 atcattttat caggagttca caaaagacgg ccataacacc ttccattcaa caggagatcc
    34021 atcagaaaac aagatccacc atttgatcac agaattgatc cacatgcaac aaggcacgaa
    34081 tgcccgtacc gatttccagt cctctgtagc tatccacgag ttgctgaatg aactgatttt
    34141 gcaaaagtat cagctagatt tcgaagaaga ggatattccc gcctacgttt acaaactgaa
    34201 gcaatactta gacgaacatt tcaaagaaac gattacactt gatcaattag aacaacaatt
    34261 cttattgaat aaatatcagc tgagcaaaga tttttccaaa tttatcggtt ctccaccaat
    34321 cgattatttg atcagcaaaa aactttctta cgctaaagac cttcttcggt atacagaatt
    34381 tacggttcga atgatttccg aagaaatagg gattgagaat tttgcttact ttagccgcct
    34441 gttcaaagca aaaacaggac tatcgccgag agactttcgt aaattcagct gattcatata
    34501 atatgtacgt cattttttca aaatattttg aaatatatag ataaaaataa aataaccgca
    34561 gtttctgctt ctatgtaaac aacagcagct actgcggtta ttttttagtg atcattctca
    34621 ttgttcatct tcaaaatgtt cataatgaac caactgtttc tgtgtatcac gtttcaaaaa
    34681 ttctttaggc gttatgccga actctctctt gaatgctttc agaaaatgcg gataagaaga
    34741 aaaaccactt ttactatata cagcactaga gctgcggtat tctcgcaaca agtgcttggc
    34801 atagatcagc tttttcttca ttacatactg atgaaacgta aatccagtgt actttttaaa
    34861 ttctcgtgtg atatggtatt tgctgacaaa aaactgcttt tccatttcct ctaaagaaag
    34921 cggatgagat aaattttttg agatatattg taacatttgt tccacacgct cattaggaac
    34981 tacagcattc tgttcttgcg cactctcttc tttttgtaca acttgattga gaaaaatcaa
    35041 gtattctacc agagcttgtt catattttat gtcttcaccg taaatttttt tatcaggagg
    35101 atggtcgata aatgataatt tttgcttcaa tacttcctgt tctatttgca atacacgact
    35161 cctcttctct cccattggtt gaaaacaagc cgatagatta gaccatggcg tggaacgact
    35221 ttctagaaaa gaaggggtga caaagatata gacacgctcg aatacatctg aagattggcg
    35281 gacgatacga tgcaaatcat tggaatggat gagcagcaca cttcctgctt tgatcgtaaa
    35341 ttgtcgtccg ttcaaataaa aagtcgcttc tccctttaat gtagcatgga tctcatagaa
    35401 atcatgatga tgatagatcg ttttattttg gctgacgaca tctttgacat gatagatttc
    35461 atactgatca gaaaccatcg tattgacttg ttcaccgctg attttctttt ggagcatgtg
    35521 ccgcacctcc ttttgttcac ttatgcacaa ttttattata ccacgttttt tgtaatcaaa
    35581 aagagctgct tttatatact cttataaaaa agaagagacg tggcatcttc tagccccgcc
    35641 ccctccattc taaatattag ttaaccaacg tcttctttac attattcaag aagacttctt
    35701 ccataacgaa tcccatcatc ttttgtttct tcaagaacca catacaggta cggacgattc
    35761 ttcatgatga tttctttgat ttttgtgtac tcgatcacac cgtctcccaa atttaccggc
    35821 acgatttttt cctgttcgac acgaaaatct ttcaaatgaa ccgcacatat cttttcacca
    35881 aaacgttcaa aagcttcttc taccaattgg acttgttctt gataatcact acttgtgatc
    35941 aaattggtcg gatctaaaat gattcctaga tagtccgaat ctactgcttg gatcagctgc
    36001 tctacccttg ctaaagaata caccggatga ttcaaacctg gttctatccc caccatcatt
    36061 tggtattttt ctccagcttt taccaagcga cggataacgg acacagcttc agcaaatgct
    36121 tcatctgtga aattttcttc cgtatattgg atttccggca aaacacatcc agtttccgaa
    36181 gcaaccatcg acgcaccaaa ggaagaagca tagcgaagat actgctcgaa ctttgtcagc
    36241 aactcttgtc gtatcgttaa atctggatga atcatattga tatagcaact taaaattcct
    36301 acggtcactt cttgcttttc taaaactcgt ttgacataat ttcccatccc agaattgatc
    36361 tcatttgcgc cagaaggtat ttcaggaaac gaaagaccta aggcgagctg cagtgtatga
    36421 atcccttgtt cttttgtttt ttctgcaaga tcttctatcg tttggacttc ggaaagatca
    36481 tgtcctctaa ttgtaatgtt caacataact attctcctaa ctcagcatat tgggtagcca
    36541 catgacgatt tgcgggaagt aagtaatcaa aaacaagacg actaagataa ccccataaaa
    36601 aggaagaagc ggtttcagca ttgcctctgc ttttacgcct cctacacttg caccgacata
    36661 tagtcctgta cctactggcg gcgtgatgga gcctacacac agattcatga ttgaaaacaa
    36721 cccgtaatgt acaggatcaa cccctacact tgtgacgatt ggtaaaaata ttggtgtaaa
    36781 aatcaagata gcaggcgcca catccataaa catacccacc agtaataaca caagattgac
    36841 taataacaga ataataaatt tattatccgt tacgcccata atcaacgaac taattgcctg
    36901 aggaataccg gtaaacgcca tggcgaaaga catcatggaa gaagtagcca ataagaacat
    36961 gatcgtccct gacattgcta ctgattgaaa caacatatta ggtatatctt ttagttttac
    37021 cgttttatag taaaacatag caatcaacag ggagtaaatg acagctatcg ctgaagcttc
    37081 tactgctgta aatatccccg tcaagatacc accgataatg atgacaatca atagaaggct
    37141 aggaatcgcc tcttttatta tttttccagc atgagccatc tgtccttttt cgacaacagg
    37201 gtatttattc tttttagcaa cgatgaatgc tactagcatg attcctagcg cccacaaagc
    37261 gcctacgaga taacctccca taaacaaagc agagatcgaa gcaccaccag caacaagtga
    37321 atacatgata aaagcagtac ttggaggaat gaccatacct gtcggagctg acgcgatatt
    37381 gaccgctgtc gcaaacttcc ggtcatatcc ttcttccact tgctgaggga tcaacactcc
    37441 gccaatcgcg gtagaagcgg caatggccga gctagagatg gaaccaaata aagcattccc
    37501 taacacattc gtatgagcca aagcccccgg cacgcgcccg acgaacaaca tcgcaaaatc
    37561 cactagtttc ttagctatcc ctccattatt catgataatg cccgagagaa cgaaaaacgg
    37621 aatagcgatc agcgaaaaac tattgatact tcccaccatt ttttgagctg aactaaaagc
    37681 agaaacatca aacggtataa ctaacaataa agtcatcatc gatgagaatg caatacttat
    37741 agcaatcggc atccctaata caagcaacac aacaaataca aaaaataaca ctaatccggc
    37801 ttctattgcc attttattcc tccattttta tattttttct tgaattgctg gcttgttgct
    37861 taatgtatcg attagattga tcaggctata gataatgatg agtacccccg atacaggtac
    37921 tgacagataa acatatccca taggaatact taaagaaggc gagatctgcc ccattgtcaa
    37981 tgtgacagca ttccatccgc catacaccat gatcatcgca gcaaatacca gaaataaact
    38041 ttgaacaaca acttctaaaa ttttactttt tttgccagtt aatttttcac ttaaaaccgt
    38101 tacggctaaa tgtgcttttt gtcccacaac atacgcggaa gacaacatgg caaaccagac
    38161 aagaccgaat cgaacaagct cttccgtgac cgtatttgga tttcctaaaa ccgttctgga
    38221 aaatacttgc cataaaacaa cagcgaccat cccaacaagc aaaacagctc ccaggatctc
    38281 taataccttg tttaaaatca ttttcatcac agctacgctc cttgctcttg gatcttttga
    38341 taaaggtctt tgtaggtagg atcgtttttg aatatatcat gaagtggggc aactgcctct
    38401 cgaaacggtg tattatctac atcattgaat tggactccat gttcttcttg tgctaaagct
    38461 ttttcttctt caattgcttt tgcaaaagct tcaatttcaa aaaccgttga ttctttcgct
    38521 gcttcttcaa tggctttttc ctgctcatca gttagctgtt ctttgatggc atcattcatg
    38581 atgatgatat ctggcacacg tgtatgctca tcataggaat agtatttagc cacaccacca
    38641 tgtccagctg tatacaagac aaattcattg ttttctgatc cgttgatcaa attggattga
    38701 agagacgtgt acacttcatc acttcccata ggtacgggcg aaccgcctaa aagctctacc
    38761 attttgatag cggtttcgct ttgcatcaca cggatcttct ttcccttcaa atcttccggc
    38821 gtatggatcg gaccgtctac catataaaag ctgcgctgac cagaatcata ataggtcaat
    38881 ccggtaaatc ctaaattttt ggtcgattgg tagattggct ccattatttc ttcattatcc
    38941 atcaccttaa agaaatgttc ttcatcattg aatagataag gaactgaaaa aatcgaataa
    39001 tctttagaga aactttccaa cgctgaggca cttacttttg tgaaatctac tgctcctgac
    39061 tgtgtcaatt cgatcagttc acgttctcca cccagttgtc catctgggaa atactggatc
    39121 tgtacggaac catctgtttt ttcttttact aaacgcccaa attcattcat tgcatcaatt
    39181 actggctggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacatctgca
    39241 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
    39301 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
    39361 ataataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
    39421 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
    39481 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
    39541 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
    39601 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
    39661 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
    39721 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
    39781 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
    39841 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
    39901 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
    39961 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
    40021 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
    40081 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
    40141 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
    40201 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
    40261 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
    40321 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
    40381 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
    40441 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
    40501 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
    40561 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
    40621 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
    40681 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
    40741 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
    40801 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
    40861 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
    40921 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
    40981 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
    41041 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
    41101 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
    41161 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
    41221 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
    41281 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
    41341 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
    41401 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
    41461 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
    41521 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
    41581 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
    41641 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
    41701 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
    41761 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
    41821 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
    41881 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
    41941 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
    42001 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
    42061 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
    42121 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
    42181 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
    42241 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
    42301 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
    42361 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
    42421 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
    42481 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
    42541 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
    42601 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
    42661 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
    42721 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
    42781 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
    42841 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
    42901 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
    42961 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
    43021 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
    43081 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
    43141 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
    43201 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
    43261 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
    43321 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
    43381 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
    43441 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
    43501 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
    43561 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
    43621 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
    43681 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
    43741 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
    43801 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
    43861 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
    43921 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
    43981 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
    44041 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
    44101 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
    44161 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
    44221 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
    44281 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
    44341 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
    44401 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
    44461 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
    44521 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
    44581 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
    44641 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
    44701 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
    44761 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
    44821 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
    44881 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
    44941 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
    45001 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
    45061 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
    45121 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
    45181 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
    45241 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
    45301 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
    45361 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
    45421 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
    45481 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
    45541 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
    45601 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
    45661 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
    45721 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
    45781 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
    45841 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
    45901 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
    45961 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
    46021 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
    46081 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
    46141 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
    46201 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
    46261 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
    46321 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
    46381 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
    46441 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
    46501 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
    46561 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
    46621 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
    46681 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
    46741 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
    46801 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
    46861 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
    46921 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
    46981 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
    47041 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
    47101 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
    47161 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
    47221 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    47281 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    47341 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    47401 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    47461 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    47521 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    47581 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    47641 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    47701 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    47761 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    47821 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    47881 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    47941 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    48001 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    48061 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    48121 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    48181 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    48241 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    48301 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    48361 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    48421 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    48481 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    48541 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    48601 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    48661 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    48721 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    48781 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    48841 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    48901 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    48961 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    49021 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    49081 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    49141 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    49201 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    49261 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    49321 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    49381 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    49441 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    49501 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    49561 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    49621 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    49681 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    49741 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    49801 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    49861 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    49921 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    49981 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    50041 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    50101 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    50161 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    50221 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    50281 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    50341 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    50401 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    50461 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    50521 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    50581 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    50641 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    50701 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    50761 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    50821 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    50881 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    50941 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    51001 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    51061 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    51121 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    51181 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    51241 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    51301 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    51361 ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg
    51421 tgtgatccaa tttgatacac cagaaaatat tttacgtcat cctgcaaacg atttcgtaga
    51481 agagttgatt ggagaagatc gcctactaca agccaagcca aatgcgacaa gagtcggcga
    51541 agttatgcta aatacagcaa tcacgattac accggaaaaa tcattacaag aagcaatcaa
    51601 actgatgagg gaaaaacgtg tagatacgtt gttagttacc gataattcga atgtattaaa
    51661 aggatttatc gatgtagaaa cgttgaataa aaaacgagga aaagtatcca gtgtcggcga
    51721 cattttgaat aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg ataccttgca
    51781 acgcatttta aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga acaaagtcgt
    51841 aggtatcctg actcgtgcat cattagtgga tatcgtctac gatgtgattt ggggagaaga
    51901 tcccgcaact actgatgttg cagagtcagc tactcctgaa gaatctaagg aggtctgacc
    51961 tatgcagcag ttttttcatc agcacggttc agaaattctc tcaaaaggtt ttgaacatct
    52021 ttatatttca ggacttgctt tgatcattgg tatcatcatt gctgtaccgc ttggtgtctt
    52081 actgacacgt tttccaaaag ctgccacgat tgtgattggc ttaaccagtg ccttgcagac
    52141 aatcccttct cttgctttat tggcattgat gatcccgcta tttggcgtag gtaaattacc
    52201 tgcgattatc gcactcttta tttattcatt attgccgatt ttacgtaata cgtacattgg
    52261 gatgaaaaat gtcgattgga attatcggga tgtggcaaaa ggcatgggga tgactgagtt
    52321 ccaatccatt ttttccgttg aattgcccat tgctatgcca acgatcatgg ctggtattcg
    52381 tttagctgcc gtttacgtga ttgcatgggc tacacttgcc tcttatatcg gcgctggtgg
    52441 tttaggcgat ttgattttta gtggactgaa taattaccaa ccagatctca ttttggcagg
    52501 tacgatccct gtaacaattt tagcactatt ggctgattgg cttctaggtc tcttggaaca
    52561 ccggctgaca ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat actaaaaatg
    52621 accatggttc ttttctctct tcttatactt gcgagttgtt ccttccctgg tcttgccagc
    52681 aatacagatg aagacaccat ttcgatcaca ggtggaatca cttcagaagc acagatctta
    52741 gcaagtctag ttgccggaat ggttgaacat tatacagata aaaataccgc aatcatcaac
    52801 aatctggcta ctacaactat caaccatcaa gcgatgatga acggagatgc ttcaatttca
    52861 gcggctcgct acacaggaac agatttaacg actacattga accttcctcc tgaaaaagat
    52921 ccgaaaaaag cgtttgctac cgtgaaagat gaatttgaga aaagatacgg tcaaacgtgg
    52981 ttcccgtcct atagttttga aaatacgtat gtctttcttg ttcgaaaaga caccgcacaa
    53041 aaataccatc tatctaaagt cagcgatctg aaaaatgtag cggatgaatt agtagctggt
    53101 gtagatactt cttggatcaa tcgtaaagga gacggttatg acggcttcca agaaacttat
    53161 ggcttctcct tcaattcgat attacctatg cagattggac ttgtttatga cgcagtcgaa
    53221 gcaggaaaaa tggatattgt tcttggctac tctacggatg gacggatcgc cagttatgat
    53281 ctagtcatgt tggaagatga tcgtcaattc ttcccaccat atgatgctgc tccagttgta
    53341 gacaacaaga tattagctga tacgcctcat ttaaaagaag ccttaaataa gttaggtcat
    53401 accatcagta cggaaaaaat gcaggaattg aactacgaag cagataataa tctagtggaa
    53461 ccatcagtag tagcacaacg tttcttacaa gaaaaccact attttgaagg gaagtgaagc
    53521 aattggataa aatgaatgtc tttcaacaat ttttctatta tttccaggaa aatggctcct
    53581 atatctttgc acaatttatc cgtcacttcc tgatttctat ctatggtgtc ctctttgcag
    53641 cagttgtcgg gattccggtt gggatcatga tttctaggag acgtaaatta gcaaactggg
    53701 tgattcgttt ggcgaatatc atccagacga tcccttctct agccatgatc tccattttaa
    53761 tcattggatt aggcctggga gtcaatgtcg tgattgttac tgtatttttg tattccctct
    53821 taccgatcat caagaatacg tataccggga tgatccaagt agacaaaaac atccttgatg
    53881 tcggcaaagg catggggatg actgctagac aacgactatt catggtagag ttaccactct
    53941 ctgtttctgt tatcatggca ggcataagaa acgcgttagt tgttgccatc gggataaccg
    54001 cgatcggtgc attcgtcggt gctggtggtc ttggagatat cattatccga ggaaccaatg
    54061 caacagaggg cacatcgatt attttagcag gtgcattgcc aactgcatta atggcgatca
    54121 tcacggactg gcttttaggt attttggaaa gacggctaga tccagccagc cgtacatcac
    54181 gctgatgtta ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc aaaggctttt
    54241 ttagttctgt catttttaag gcaataatcc actctgattc ttccctcctt atgggcaaag
    54301 agttgctttt cttagctatt tatccattcc gtatactcca acttcatact gccaacgggt
    54361 tcatatctgc atccaccgac taagtttttt tcttatccgc taaggcattt tgccaatcca
    54421 ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc gtctgcttgt
    54481 tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta agccttttcc
    54541 atcagtcctc gattgttcga tgccttcagc tgcatcatca aaactggtga ctgccgtatc
    54601 ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct tggctaaaaa
    54661 catatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa accggtacac
    54721 gattcttatg gactgcatcc gtcgcaacta gacttggcga tacgatgatc cttcaccaga
    54781 agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac attggcgtaa
    54841 aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc cgaaagcgcc
    54901 acgactttaa cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc tttggatacc
    54961 cgctctgcta aaggaacgac actggtattg tatttcgtat ctgtatcttt tccaaaagac
    55021 catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc cattgctttt
    55081 ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt cgcctcactt
    55141 tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc aattttactg
    55201 attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca ttccattcct
    55261 aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag aaatcatgca
    55321 ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg ggagactctt
    55381 tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc caggcagaat
    55441 aaatttctac aactttattt tcctcatcaa ctttgtaaac cactcgatgc tgtctattca
    55501 gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac tgcgtgggca
    55561 tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt aaattcgttt
    55621 gcttgatttt tcttaaatcc actttcgcag agtttttgat tgcgactgta tagttactca
    55681 tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt ctttttcgcg
    55741 gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt gaatcgctct
    55801 ccagtcttcc agcccaatca atacagcatt gtttccacta cgatcactaa taatctcgat
    55861 ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac gagcagtgct
    55921 aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa ttatagcgta
    55981 cttgattaag tacgtcaaaa aaatacaata aagcacttaa attcagcaaa cttttcctct
    56041 tttttacata gagaaaaaga ccccttttta gggaggatct ttgtccacgc tcacttctta
    56101 ctcctttacg actagcggta gccaaacgta aagtaattgg taacaacgta tcatctttag
    56161 caaaggtcga atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg aaggttcctc
    56221 gttgcagtgt gacttgattg atatcttgaa taacctgagg atacgcacga aagatttgta
    56281 acggttattc aaaactgatt ttggccgtca agtcttctac tgcctcaaaa atgattgcct
    56341 ctttcgtctc ctgtcattca tgttcactat cgatttgata cttgcttaga ccgttgggtt
    56401 tgtgaatcac caattaaaaa tggcactgag ttatggtaac aaaaaatggt cacttgacga
    56461 ggctgagatt cctacttatg aagacacatg gcacttaaga gtcgaggtaa aggaagaagc
    56521 tgaaaatata ggaggctgta cattatttgg caaaggattc ggaaattatg atcaagacat
    56581 caaaatcaaa atgttttaca gctaaatagg aagaataata acatttcata cgctgttccc
    56641 atctttaatg gcttatagag ggcaccttta gctgagaata agacaaaaca gaccctctag
    56701 cttcaattaa gagctagagg gtctgttttt gattttaaaa cttggtttat ttcttcaaat
    56761 ggtagctata ggtgctgaca acgatatttt cacgccaaga caatcgcaat cttaaccgca
    56821 aacttgtttg gttatggaga atattctgcc agcgtctgtt tggtacaaat tgaggaatca
    56881 acacagtagt tgtatagttt tgtttcgctg catttttact tacgatatct acataacgga
    56941 taattggatt ttcaatcgag cgataagaag agtggacgat tgaaaaacgg acatctggga
    57001 aatgcttttt aaattctgct tcgatttctt tttctttttc gacatcttcg tctagcgaaa
    57061 catgcatcgc aacgacataa tcaccgatcg aacgtgcgta attcaacgca ccgatattta
    57121 cccgtgtcac attcccgact aatacaagaa cggtattccc gtcataatca tgtagttttg
    57181 catcattttc taaacgaagc tgttctgcta cttttttata atgatcatgg atcttataaa
    57241 aaatgaacat aacgattggc ataatgatga agaaaggcca aatgtcccct aaccgataga
    57301 caaacaaaat cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg gcaatcgatt
    57361 tgcttaacca tctttttcct tcttttttcc atttgacgac catccctgtt tgagataaag
    57421 caaatggaat gaacacgccg atagagtata acggaatcaa gcgttctgtt gagccatgga
    57481 aaataaacag caatacgatc gaacctagcg ctagtgtgat aatcccattg gaatagccta
    57541 gtcgatcccc acgatcttga tacatgtgag gcatgaattt atcttttgcc agattgtatg
    57601 caagaactgg aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc agcgctgtag
    57661 caaattgaag gacgtaatac aaaataccat gtccaaagac agctttacca atttgagaca
    57721 aaacagtgac ttctttttca ggaacgatcc cataccaata gttgataaat gtgatcccca
    57781 cgaagaagaa acctaagatc aatgccatca tcgtcaatgt agcagcggca ttttttgctc
    57841 ttggcttctt aaagaatggt acagcattac taatcgcctc caccccagtt aaagaggaag
    57901 aaccagaaga aaatgctcgt aaaatcaaag cgatagaaac acccggcaca gccgctcctg
    57961 gaagtgcagt agcttgcaat ggcacagcac ctgtcacgat attatataaa ccaacaacga
    58021 tcagtaacga gatcacaagg ataaatgtgt acaccggaaa taataggaac gatgcagatt
    58081 ccctcagccc acgaagattc agcatcatca gtagcaaaac gattgtcaca gaaatagcga
    58141 cttgatgacc gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct gctgagacag
    58201 atactgctac tgtcaacatg tagtcgatca ataaagagcc accggaaatc aagccagcat
    58261 tctttccaag attctcactg ctgacgacat atgctccccc gccatgagga taagcatgga
    58321 tgatttgacg atatgatagc gttaatgaaa tcaataaaat aataacaaaa gcggcaatgg
    58381 gtagcgaata ccagatggct gcagcagaca aagcgactaa aacgacaacg atttgttccg
    58441 taccatatgc aatagatgat aacgcatcag acgacaataa tgccaaagca gcaaatcggg
    58501 ttaatttgtg ctcatcattt tcagcagatt ttaaaggctt acctactaac aaccttttta
    58561 agtaatccaa tttctaaact cctttacaca caattttact tcaagtttta tagtcgaaaa
    58621 tttatagtaa acaagatgtt atagtacgct cactgacctt aaaagtcaat aaactttaag
    58681 aaaataattg gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt taatacagca
    58741 aaaatctaac atttttttat gggctttttc tttgaattct ataataaaag gatagaactt
    58801 gacttagaaa atcaagttct atcctttttg atatcttaac ttttggaaga gagatgtaag
    58861 gctgctttct acccatcatt cttactactc gaagcatagc gcagttgtca caacctcttg
    58921 ataaacggtg tttaaaagct tgcatctgcg gtaataagcc caaccaaatt aaaaacataa
    58981 gaagctgttt ttatttggtc gttcttatta cttgatgcag agcagctttt ttacaacctc
    59041 ttggtacacg gtgttcaatc agctgaccct cggtattagc tcaaaaaaac ggcaaaatat
    59101 gaggaactat ttttccattt ttttctctaa tactcaggtc aaaacactga tttcacaacc
    59161 tcttttttta atgaaaaata tggctctcat gcgccgcttc tttgaatttc gtcatttttt
    59221 cttcgatgaa cggatagatt gccgttccta cgattccaaa gacaacaaac aaaccgatca
    59281 tgatccagat tgctttcgta gcatttggcc aataaattcc tcctgcagat tcccgtaaca
    59341 agtttaccgc atgagtaaat ggcaagaacg gattgatcat ttggaagaat ttccctgaga
    59401 cttggatcgg atagttcccg cctccgccag agatcgacag tactaagatg ataatagcga
    59461 tccctttacc gacattatcg aataatgcag ccaagacata gacgatcatc atgaacgcta
    59521 aggcgatcaa gacagcaaat gacacgctgt agaccggatt ttggacatct actcctagta
    59581 agaaaatatt ccctaatgta acgatcaagc tttgcgcaat cgccattgtc aaaaatgtca
    59641 gcatacgagc aacgaattgt tctcgtttcg taaaattgtt tcggtctttc tttcctaaat
    59701 agtattctgt cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga catagtgcag
    59761 tataaaacgg ggtgctagct gaaccgttat ttgcgattgg ataaagcgca ttggtttgta
    59821 gctcaacagg ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc aataatttga
    59881 tgacttcgcc tagatcgact tcttgttcgc cttttcggat cgcgtctgct gctttttgga
    59941 taccagcttt gatatttggc cagtcattgt tgatcaagtc cgcagcagcg ttcacagctg
    60001 tttcgacttc tggcattttt tcatttactg ttgtcaatgt accggtgatt tcatttttta
    60061 gtgctggcca atcattttgg ataaaatcag cggccaatcc taatttttgc tgaatgatcg
    60121 gtaattcatt gttgtatagg tctgcccctt cattgatgcc attgacgatg gtatccatat
    60181 ttccattcag cattgtattg gcatcatgga tttcttgtcc aattgcaggc atttcttttt
    60241 gatatttttc taaaattgcc actgcattcg ttacagttgc tttcgttgag ctgagaagcg
    60301 aatcaaagtc gatctggtcc gctttgctca gtgcgccttg tgcagtccca atcgtatcga
    60361 tgacttggtc tagtagagaa gtgacagtgg aactgatttg acctgtattg ataccagcaa
    60421 tgatgccatt gatattatta acaacggcat caatttgatc tagcaattca tcaatagcct
    60481 tcgtgtcttc attttgaaca gctgtgttca aagcagatag gcggttttgc agattgctta
    60541 gcaagggttt taattgattt agctgatcaa taattggctg taatttcgta tttccagcac
    60601 ttgtttgcaa tttagtcagg aactcgacca gtttatcgat tgcattttgt tgttgagata
    60661 ttccgtcaat aaagccgttc aatacattgc tgatgttttt cttttcatca tcagtcagct
    60721 taccatcttc aatagctgtt cttacagtat ctattaacga acctgtcgtt gttgccactt
    60781 gtccaattga cttcaatgta acatctacac tgtttgtgat gcttggtatc gcatctttca
    60841 gctgagaggc acctgatttt gtttggtttg ctagatcact tgcctgattt tctaaatttt
    60901 tcacatctgg caagatatct tgtacttgtt ggatgatcgt caagccttgt tttgcttcat
    60961 cgattccttg gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc tctgctagtt
    61021 gcttaccggc attttggatc tctgggattt tttcttgcaa atcaaggatg atttttgctt
    61081 gatctttcaa ctcaggcatt ttgtcattca atgcaacgac tttttcaccc atctcatcta
    61141 ctttagggat gtaatccacg aattcatttg ctttattcag tttttccttg atttcaggta
    61201 gttgactttg taattcgagg acttgttttg tgtagccgtc gatcgtgtcc aaattttcat
    61261 ctgttgataa aatcatatct ttaactttat tgatacttac taggttcgta tcgatgtcgt
    61321 aacctatctc attgaatact ttcaataaag tcgaactggc tgttttgata aattcatttg
    61381 tgatttgcga ttggatcgaa cttgctcctt tatcggtgat tttcggtgca atcgcattga
    61441 ttttttcatt taccgtatat tcgatttttg gtttcttgat atcaccacta gtaaaactca
    61501 gcagatcttc tgagaaatcc tttggcaagt aaatacctgc gtaatacttt ccggaacgga
    61561 ctccgtcttc taattgttct tttgaatcaa cgaattgcca tcctaattgt ttatttttat
    61621 gaagcgattc gatgacttgc tccccaatgg ccacttcttt accttgaaac tctgctggct
    61681 tgtctgcact gtaaacagca attggcagct ctccagtatt tccatatgga tcccataatg
    61741 ctttgatgtt aaaccacgca tatagcgaag ggatgatcat gatcgcgatg atcagaagtg
    61801 ttgcaatcgg gtttttgaaa atccgtttcc agtcaagcaa atataatcga agcgtatttt
    61861 tgattgactg catagttctc ccccttcttc ttttttcagc tgaattactt taccatgtat
    61921 ttcatacttt tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca tatcacggca
    61981 attgtctcat tgtaatctct tacatattga gaaataatta aggattacag cctttacttt
    62041 atcatgaaat aaaagggaaa gataatttta tgccaaagtt tttatctatt atacgtatac
    62101 gcaaaaaagc taaaacattt tttgtctttg cccctacaaa aatgtttcag cttttttcgt
    62161 tttttattcc tgtatttctt atttttcgaa tgacaggatg attttcccag caggaggatg
    62221 tccttcaact agctgatgtg cttggatgac atgcttaagt gaagcagaca gattcattga
    62281 aataaaaaca tgaaaggctc ctttttggta ggctttgatt atgccagcaa aggcttctgc
    62341 atctagatat tcctttcttg gaacaaacga ttcataaaag ccttcctttt tttgccgcaa
    62401 atctagatct gggaggtcat ttaatgcaac atacctgccg cctggtttca tgatctggat
    62461 gccggtttct cctttgatac ttcctttcgt cgcatcgatt acaaggtctg cctgatcagc
    62521 aaattgcaga cctggatttg ttttatcata agcagcaaat gctgaagctc ctaatttttt
    62581 taccttttcc tcatttttac tggatgcact ggttaagatt cggattccct tttcatggag
    62641 aagctggatc agacttgacc cgaccgcacc tgatgctcct agtatcatta cagtatctgt
    62701 cggctggatt tccgtcaaat ggttgattag attataagcc gtaattcctg gcgtgaccat
    62761 tccggctgct tcttcccaac tcattttatc tggaattttt gctacttttg ctgatggcaa
    62821 cacgacttct tctccgtaag tcccgccgac tgcatggaca gctactcgat cacctacatg
    62881 aacagttgtg acatctgaac caatctctgt cacaatacca gcgccatcat tacctggtac
    62941 ataagggaat ttcaatgttc gtatctcttt catttctcct aaacgcaagg caacatcata
    63001 aggattcaca ctgaatgctt ttatttctac acgaacatgt ccagaaccga cttctctggg
    63061 atgagcatcg atttcttcaa aaacatcttg tgcttctccg taaccgttaa ttgcaaatct
    63121 tcgcatggtc accatctccc tttttcttta agtttacgtt tatgccttta ttttgacaaa
    63181 aaaaatgtct tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt ttttcgcgta
    63241 acgtgttaaa ttatttatct aaaagcgtaa agattacgat ttacgctaat aaaaaataat
    63301 tggaggaatt tgttgatggc taaaaaatca aagattgcca aagcaaaaaa acagcaagaa
    63361 acgattgaac gctatgctgc tttacgccag gaattaaaag cgcaaaaaga ttacgaaggc
    63421 ttgcgtaaac ttcctttaga tgctcgtcca gaacgattga aaaatcgtga tttaatcgat
    63481 ggacgccctc gcggatacat gcgtaaattc gggatgtccc gtataaaatt ccgtgaatta
    63541 gcacataaag gtttgattcc aggagtaaaa aaagccagct ggtaatccaa gtctggctgt
    63601 aatgtaaagg aggagggcgc atggaccact ccttggatca tcaaaaaaga tacgttcgct
    63661 tcttctagca gaaatgctgg tactagaagc agacgtatct ttttttacgc acagctggtc
    63721 cttttttata tgtaaaggct gcatggttac ttcgctatat ttatttagta acaggagtca
    63781 gtgcttcgat acccacacta attttcaatg ctcggtcaac ttctgccata atgggtcgat
    63841 ccaaatggca gaccttttct ttcaatcttg atttatcgat cgttctgatc tgctccaaca
    63901 ggataacgga gtctctttct attcctgttc cagtagcttt gatcccaatg tgagtcggaa
    63961 gcttaggttt agccattttt gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt
    64021 tacctagatt attttgaata accaaaactg ggcgtgttcc gccttgctcc gatcccacga
    64081 ctggagaaag atcagcaaaa taaatatctc ctctttttac cataggatcc tccttattct
    64141 tgatattctc ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct
    64201 aattgatctg ctatatcctg catagtgatt tctttgtctg catttttgcc gataagcgtc
    64261 acttttgttc cgacaggaaa ttcttgaggc aatcggatca tcagttgatc catacataca
    64321 cgcccaatcg tttcacagta gttcccttct accaatagag aaaaaccctg catctttcgt
    64381 ggccagccat ctgcataacc gatcggtatc gttccgatcc actctgcttc aggagtgata
    64441 tatgtttctc catagccgat tccttcacct gctgggagtt ttttgacttg aatcaattca
    64501 gaaaccagct caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg cccagaggga
    64561 ttaagtccgt acattgctac tccataacga atcatattgc cgattgtttc atcatgccat
    64621 aaagccgttg cactattgct gacatgtaca tatcgaggaa gttcgggaag ttttttcagc
    64681 acttctataa agcgttcttt ttgcagattc caatatgtat catctgcctg atctgctgta
    64741 gaaaaatggg taaataaacc ttcccattct aatacacgac tttctttgat gaatgcagct
    64801 gcctctttta cttcttcagg tgtacaaaat ccgatacgac ccatcccagt gtctgctttt
    64861 agatgaatcg aaagaggcgt ctctagctta cttttctcta aaacagcagc tgctgcttgc
    64921 aaccattccc gtgtacctgc agttactgat aaatcatgag caacagctac agtcagtgcg
    64981 tcgtaaggaa ccatattcaa gataaggatg ggctgagtaa atccagcttc tctcaactcg
    65041 actccttcat ctaaatttga aacacaaaag cccgaagctc cacctgcaac tgctgcctca
    65101 gctgtttcaa tagctccatg accgtatccg ttcgctttta ctactgcaaa caattctttt
    65161 ccttgcggaa gtcttgcaac ttcatttgct acattttcag ttatagcttt tttatgtatc
    65221 actgctttcg ttgggcggtg ccaagcgaca accatcgttt ttccttcttt ctagtcttcc
    65281 aaaataattt gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca tacattgcct
    65341 tcatgaggtg aacgcgtcac aacaggctga ccattttcat tttttaaaat ctcgacatct
    65401 tgaaaggtaa cttttccgat cccagttccc caagctttag agaaagcctc tttgcaagcg
    65461 taacgcccgc ctaaaaattc tacttggcga tgaaaaggca aggactggaa tagtttcatt
    65521 tcatcagagg tcaagattcg tgcaatgaat tttggttttt cttcgatcag ccgagtgatc
    65581 ctaggaagtt cgactgcatc gatcccaatt ccttttatca tatgcttttc tcttttcaat
    65641 aaatttagaa cactctcatt ctaccaaatt ataaccattt tgccacaaat aagtccacaa
    65701 ataacaaaaa tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta
    65761 tttgaacgga ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc atctttttgg
    65821 ctgttattat ttttgctata accgcctttt gatttgtttc cacgataatt accgccgtca
    65881 cgacgattac ggttgtttcc gccaccacgt ttgccatttt tattgtatcc ttttttctgc
    65941 attggcaatg ggcgttctgg tgtgatcttc actggcactg catcagctgg atcttttgct
    66001 gtcgttttta gcaaaagtgc aactaaatct tgtgcagaat attcttctaa taatttatct
    66061 gctgtttgca ggtatttatc tagaccattt tctgcaagtt ttgtttcgat ttgttcaact
    66121 gcagcaccta attgaccttt gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc
    66181 cgttttttcg tcaagttttc aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa
    66241 gtcacagaca taccgccttt tccggcacgg cctgtacgtc cgatacggtg aacgtagctt
    66301 tctggatctt gagggatatc atagttgtac acatgagtga cacctgaaat atctaatcca
    66361 cgtgcagcaa cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa tgaacgcaat
    66421 acgctcattc gtttttgttg agacaaatcg ccatggattc cttctgcacg gtaaccgcgt
    66481 gcttctaatc cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt
    66541 aattcaggtg tttgaacatc aaacaagcga gtcatgatat cgaatttttc gtattctttt
    66601 gcgcggacat agtattgatc gatcaagtca gctgtcattt ctttcgcttt gatcttcaca
    66661 tgatgtggat ttttcatgaa tttaacgcca atattcttga tagctggcgg catcgttgct
    66721 gagaaaagta atgtttgtct ttgatcagga acttgtgaaa tgattttttc gatatcttcc
    66781 aagaagccca tattcaacat ttcatctgct tcatccaaaa ctaatgtttg aacggttcca
    66841 agtttcaatg tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc cacaacgata
    66901 tgtggacgat ctttcaagcc acggatctga cggccaatat ctgctccgcc gtaaacggct
    66961 tgtacacgta cttttttgtc gcgccctaaa cgatataatt cttcttgtgt ttggatagct
    67021 aattcacgtg ttggtgcgat aactaaacct tgtaattcgt gacgatctgg atcgattttt
    67081 tctaacattg gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata
    67141 acgtcttttc cagctaatgc tagtggaatc gtttctgctt ggataggcgt tgcttcttca
    67201 aaaccagcgc gttcgactga tttcaacaat tctggtgata attctagttc tttaaatttc
    67261 aaatgaatcc tcctatatgg ttgttaactg aggatgtagc ttttttgcta ccccgtcgat
    67321 ttgttcggtc aaagtgtatt gtttcttttt gacagtggcc acgcacgttt ttatccattc
    67381 ttagcccctg tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa
    67441 acgggtcgcg ttcagcttta tcattattat gacatctggc ttcaacaagt tgtttctatg
    67501 gcaaaaccag tccaatttct cttcatcttt cggcaatagg ttattctaac atagtttttc
    67561 gttttctgca agtttacaga tttttatttc atttgctgaa gttgttctac cacttcaaat
    67621 aagcccatgc tgttgctgcc ttttagcata atactatctg ttgcttcaag attttgtttg
    67681 atagcatcaa tcaatttatt cttttctttt tcaaaataga acacaggtag ttgggggtat
    67741 ttttggttca attcttcttt tagtgcatgc atttgttccc cataaaggaa aatcatggcg
    67801 aatttctcag gatcgatatg ttcactcatt tgcgcatgca tatttaaaga ctctttccct
    67861 aattccagca tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa
    67921 gtatctaata ccagtcccat ggctgttgga tttgcattgt agacatcgct caaaatagct
    67981 gcaccatttc ctgccgttag ccattcggtt ctgttttgag tgatagaaac atgggcgagc
    68041 ccttggcgta tctcaggtat ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa
    68101 gcattcgtta cattataact tccaggcagc gggatcgtaa atgtttctcc ttctactaca
    68161 aaatcggttt gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc atttccaata
    68221 ccgaatgttg tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa cggttcatct
    68281 gcgggcacaa cgagtaatcc gtctgataca agtccgtcag tgatttccat tttggcttta
    68341 gcgattcctt ctcgcgaccc aagattttct atgtgtgctt caccgatcat tgtgattgct
    68401 gcagcttttg gttgagccat aagtgaaagt tccgtgattt cattggcgtg atccatgccc
    68461 atttccaaaa cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca
    68521 atattattgt tgtaatttcc ttgcgtctta tatgttttgt atttttcaga caagacagac
    68581 gcggttagat ctttcgtggt ggttttacca ttgcttcctg taattgcaac gacttctgcg
    68641 cctacttttt ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg atctgctaca
    68701 ggaataactg gaaaattttt tggagcttgt tcaacaggca agctccaaaa tgaagcagat
    68761 gctccgtttg tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc agcaagagga
    68821 acgaataaac ttccttcggt tatcttccgg ctgtcaaatt cgacattggt aatctttgtc
    68881 agtgcagagc attcgctgcc cacggcttgt gcaatttctt gtattgttag attcattgag
    68941 atctctccta aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt tcgcatgact
    69001 ctcacttcca agtaaatttc tctattcgta tttagaagct agaatgaacg cggttacgca
    69061 atcactccag cctcttcttt atttctcttt attcattttt ttcaaaaaaa gattgacgct
    69121 gatggtatcg attcattcct aactggatca gttcttcaat caaatcaccg tatttcaagc
    69181 ccatattttc ccataagagt gggtacatac tgaactccgt aaatcctggc atagagttta
    69241 attcattcag gaataattca tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc
    69301 ttccacctaa catcgtgtaa gctaacttcg cgtactcttg cgctttttga taaacttctt
    69361 ctggcacttc ggctggaatc tgcatttcga ttttattatt gatatatttt gcttcataat
    69421 catagaatgc tacgtctttt acgacttcac caggcaaagt cgtccgaacg tcttcatttc
    69481 ctaatacagc aacttcgatt tcgcgcgctt caattccttg ttcaacgatt gctcgagaat
    69541 catactgata ggctgttgct aaagcatttt gcagctcttc tcggttttct gcttttgtaa
    69601 tgccgacact agaacccata ttcgccggtt tgacaaacat cggataaagc aaagaacctt
    69661 cacattgatc aaatactttt ttaggatttt ctttccattg attcttaagt actggtacat
    69721 aaggaacttg cggcacacca gcagcttgta aaatatactt ggtcatgatt ttatccatgg
    69781 cacatgcact ggtcaatacg cctgcgccga cataaggcat attcaatgtc tctaagaagc
    69841 cttggatcgt tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg atggctcctt
    69901 cttctttgat ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct gtctgtccac
    69961 ttgggtccca tgaaagatgc aagacatctt tgctggcagg tttttctgtt agtaatggac
    70021 ctttgaccca ttgtccttct tttgtaataa atacgagttg aacttggtaa taattataat
    70081 aaatggcatt taaaactgaa aatgcggaaa gaatggacac ttcatgctct gcgctgcgtc
    70141 cgccatatag taaagtaatc ttcaaaagat tgtcctcctg tattctaaat cgttcagtga
    70201 tttactctca tattttagca caaaacaaaa ataaacgaat aacttattta taaaaaaacg
    70261 agccagcttc taaaagaacg agcgtcattt attttttgtc agctagatta tttgtcccgt
    70321 tttttataaa aagttatgat aaagaaaagg agggatgatg atggcgcaaa caaatccacg
    70381 cagccgcttg gcagaaatca ttcgagtgtt catcaattac aatgtagtgc caaactttgt
    70441 acagcaaaaa aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat
    70501 caagatagga cagatgcttt ctgttcgaga agatttgctt tcttctgcct tcactcaaac
    70561 attcaaaaca ttacaagata gtgtacctag cgacacattt tcaacggtca aaaagacgat
    70621 cgaaacagaa ctttctcttt cattatcaga tatattcgat gatttttcaa aatcgccttt
    70681 tgcctctgct tctatggggc aggctcatcg tgccaagctg aagaacggtg acgcggtagt
    70741 cgttaagatc caacacccta acatcgccga agagatccga cttgatcttc aactatttga
    70801 acgagcgatc cctttgatca aatacattcc tgaaactagc gtagttgatc taaaaggggt
    70861 actccaagaa gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa
    70921 tggcgaacaa ttctatcaaa agaacaatgg ctggaaagag atccgcagcc ccaagattta
    70981 tgacgctttt tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta aaaacttgaa
    71041 tcatttaatg aacgccgaaa acaaaactga aacttttatc actggaattc aaaacaagca
    71101 gctgaaacaa gaagttgcaa agcttttagt tgagaacttt atgaaacagg tgtttgatga
    71161 tggttttttc catgctgatc ctcatccagg gaatctgcta ttccatgtac taacaaaaga
    71221 agaacagaca caagctagcc gaaaaactga aacagtccat gaaaaagagt tcggttcctt
    71281 tgcttttcga gcctccactt ctgcagaaga ccctgttgcg ccatatacca tcaactacat
    71341 cgattttggt atgatggggc atctttctgc aggacttagg caaaaactca cacaagccgt
    71401 tttagcgctg tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc gactttgtca
    71461 gcaagaaggc tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca
    71521 gtattatgat tctcctattg atgaaatcaa cttgcaagaa gtattcacac atgtcgtgac
    71581 gatttgtcat caaaataatc tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt
    71641 tgggacatta gaaggggtga tccgcgtcct cgatcctgaa gtttccctca tggaagtcgc
    71701 ttcgcctttt gctcaacatt attttctcac tcatctggat gtagaagata cattgaaaca
    71761 aagcggatta gacttattgg aagggatgaa ggctgcgcct aagatccctc aacagctcca
    71821 ccatctatta gaaatgtgga cttcgggaca aggaaaagtc aatttggaat tgaaaaagca
    71881 agacaaatta ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt
    71941 agctgcattg atcgtagggt cctccctgct tgtccaagca gcacctgtag aaaacgcaga
    72001 ggtcgtttcc ttattaggga tatttacgta tgctattgcc gcattcgtca ttattttttt
    72061 ggcaatcgat gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac
    72121 agaaagaatc agcttcaaga aataagaagg ggttttacat aaattgtttc tcaaattttt
    72181 gtgaaaattc ggcttatttt tcgaagaggc tacttctgaa acaccgagaa taaaaaatgt
    72241 gaaaaagctg ttctgcaccc agtaataaga acaatcaaaa agggaacatg gaacacattt
    72301 atcctgtttc cctgttccct ttttgatgta ttcgagcata aataaaatac cttcgttacc
    72361 tactatcttc tgtacttggt aaaccttagt caattgcctt ttttaacgct tgacgaaaag
    72421 acatatgatc tttctccgta aagggttttg gaccctttgt ccttatgcca gcttttctta
    72481 actttccact atgataacgt tgttctagaa gcgtgccgat attcgcttcg tcaaattctt
    72541 ttccagagtg atggaacact cgcacaccct tcggcaatac aagtgaagct aaaccataat
    72601 cttgcgtgat caaaaagtca ttcttcttaa ccataccgac gattttataa tccgcactat
    72661 cagctccgcg gtctacgtag acgatttgga catgattagc gtattctttg gtcgtataat
    72721 ggtccacact ggtcacgata accacgtcaa gaccatagga ttctcctaat tttatgacat
    72781 cttcttttac tggagaacca tctccatcaa tgaatagcct cattcttttt cgccttcttc
    72841 cataaatgat aaacagttac taatccgctc agtaaaagac caacattgat ccattttgcc
    72901 aagataagca gttgtacact ttctatcggc actgattcat ggttcagcgt gatcaataat
    72961 agatggattg gctctcccat ttcttttgct aaaacaaaca aaagcgtcat caataaaaac
    73021 tggtggatca cactcccttt attttgagta gtatacttga caacgatccg gcgatccatt
    73081 ggaatagctg tatcagtttg ataatattcc ttatttagcc agctgcctgt tactacgtgc
    73141 cagaaactaa gcagaaagac acctatcgca aaaccatttg gaatcaagag gaccaataaa
    73201 gaagaactca gtccaagcaa ttgcatagcc aatggagacc atgttaaacg tttcaaaatc
    73261 catggtccga ctatttttgc tgcaatcaag cctgctgcat aaggcaaata cataaaaatc
    73321 gaaagttttt ctaatccgta cagtatgcca acataaaaac tgctaaataa aaaaaggaaa
    73381 ttccctagca ttccatttag aaaagtaaac agatttagcc agcttggcaa cttccattcc
    73441 ttcttctggt gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa accataaata
    73501 gcaagatcaa aaagcctttc atcgaataac aatcgggcat ttctcaaaag aaacaataag
    73561 ccaaagaata tcaaaaataa aacaagctct cgcgtagcta ccagatttct tttgatgtct
    73621 ttgaaatcca gttcgtaacg cggataatga ctaaccgtat ggtaggccat gacgtaaaaa
    73681 agtgtataaa tagtaaagct tgctgcaatc tgagtcggtg caggcaactc aatcgcttga
    73741 tacaggagaa atagccagat agctgcaaat aagtactttt tcccatccat attgataaaa
    73801 ccttgcgtct tttcgtgaaa attgaccgtg gtattagcag gtacaagcca tgctgcactc
    73861 agccctagca aaacacttga aaaataaaat aaaggaaaat aaaatacgcc taatattcca
    73921 agcaatgaac cagcaccacc aatcagcaga gcgatcatca gcaacgtgta actatctaga
    73981 ccaaatcgta gactgttgac taaaaatagt ccagtcatcc gaaaggcata aaacagcgta
    74041 aatggcaaaa tcgtcagcca attatgcgag tctgttagtc ccaaaaataa aagataagga
    74101 ataaaaataa ccgtgttact taagataaag ggcgttgttt gtgtaaaatt acgcccaaat
    74161 cgtttgagtt tttcgaacat gacaatggct ccttaaacag tcttataact aatctgctct
    74221 tcgattttag cattaaaggg gaaataaacc aattttacag aatatattct tgtaaaaaaa
    74281 aatacagaaa gaagcataag cttctttctg catttttacg gtacttgatt actcaagcag
    74341 acaagccctt ctccacaaaa gtcttgcccg acacccctgc atccggcagg tgataaagat
    74401 agaaatcaat tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc agagtccttc
    74461 tcgacccgtc gcataaattt actttaacac agttcctatg tttatgcgag agcttttttc
    74521 attttataat cgaccatttg aaatccttga ccgatatctg aaataagttc ttctgtaatc
    74581 tcaaatcctt catgcttata aacctcgatt gcttgatgat tcccctgatt cactcgcaaa
    74641 aagatctctc tttcctttgt ttgcgcagca agttcttcat accagcgaaa aactgaagaa
    74701 gtcaatcctt gaccacgtac tttatttagc aaatataatt tacttaaata aatttttcca
    74761 tttatctctt cataagcagt gtaaccgaca gtcttttctt cgctttttaa taaaaagtag
    74821 gacacacctt ctgtcagttc tttttgaatc tgtttttttg actgataagt tttcagcatg
    74881 taatccactt gtttttgacc aatgatcggc acaaaaactt ccggccatac ttcttgtatg
    74941 actgccatca gctcatccag ttcattttct gttactttta ttttttccat acacccacgt
    75001 ccttttttac attataaatg accttgcgct tgatggaata aaaagagcat gatcatgcta
    75061 tacacaccca ccgaccacgg tttcaagaaa atcacaatct gcataaattc cctaaaagat
    75121 aatttcgtca aaccagctac tagacagatc aagtcgtctg gcgcaaaggg aagcagcaag
    75181 gtaacaacta gtactttttt aatatctttc gtttttcttg tcagcaattc atcaaatttc
    75241 tgatactttt ttggcgataa aataagctgg acaaaggcta cgccatagta acggacaagc
    75301 aaaaatccga ttgcttcccc aatcactagg ccaatacaac tatataaaag cccaaatcca
    75361 ttgccaaaca tcagcatgcc cgctactgag gagatccctc ccggcaaaat aggaatgatc
    75421 acttggatca cttgcaacag aacaaatatc atcactgcat acttgccaaa ttgctggata
    75481 aacgcttgca aagacgtgct cgatcgaaaa atcccatgat gataagcata aagaatcaac
    75541 acaaagaaaa aaaacagacc aataatgggc atgatctgaa gacatttttg taaaactgat
    75601 ccttttttag acatctatat tccccacatc ttttttcatt tattaaaact gcctcct
//
LOCUS       DASJSZ010000003        70242 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.003, whole genome shotgun sequence.
ACCESSION   DASJSZ010000003 DASJSZ010000000
VERSION     DASJSZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.003"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            37..789
                     /locus_tag="U4B03_000164"
     CDS             37..789
                     /locus_tag="U4B03_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2067044.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            786..1700
                     /gene="pfkB"
                     /locus_tag="U4B03_000165"
     CDS             786..1700
                     /gene="pfkB"
                     /locus_tag="U4B03_000165"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN2067045.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1718..3649
                     /locus_tag="U4B03_000166"
     CDS             1718..3649
                     /locus_tag="U4B03_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2067046.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3760..5319)
                     /locus_tag="U4B03_000167"
     CDS             complement(3760..5319)
                     /locus_tag="U4B03_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEN2067047.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5474..7708
                     /gene="pcrA"
                     /locus_tag="U4B03_000168"
     CDS             5474..7708
                     /gene="pcrA"
                     /locus_tag="U4B03_000168"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEN2067048.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7725..9761
                     /gene="ligA"
                     /locus_tag="U4B03_000169"
     CDS             7725..9761
                     /gene="ligA"
                     /locus_tag="U4B03_000169"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEN2067049.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9867..10172
                     /gene="gatC"
                     /locus_tag="U4B03_000170"
     CDS             9867..10172
                     /gene="gatC"
                     /locus_tag="U4B03_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEN2067050.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10172..11644
                     /gene="gatA"
                     /locus_tag="U4B03_000171"
     CDS             10172..11644
                     /gene="gatA"
                     /locus_tag="U4B03_000171"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEN2067051.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11641..13071
                     /gene="gatB"
                     /locus_tag="U4B03_000172"
     CDS             11641..13071
                     /gene="gatB"
                     /locus_tag="U4B03_000172"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEN2067052.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13081..14136
                     /locus_tag="U4B03_000173"
     CDS             13081..14136
                     /locus_tag="U4B03_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN2067053.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14247..15623
                     /gene="rlmD"
                     /locus_tag="U4B03_000174"
     CDS             14247..15623
                     /gene="rlmD"
                     /locus_tag="U4B03_000174"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN2067054.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            15832..16668
                     /locus_tag="U4B03_000175"
     CDS             15832..16668
                     /locus_tag="U4B03_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2067055.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            16817..17038
                     /locus_tag="U4B03_000176"
     CDS             16817..17038
                     /locus_tag="U4B03_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2067056.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            17039..19756
                     /locus_tag="U4B03_000177"
     CDS             17039..19756
                     /locus_tag="U4B03_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEN2067057.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            19749..21704
                     /locus_tag="U4B03_000178"
     CDS             19749..21704
                     /locus_tag="U4B03_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2067058.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            21694..22830
                     /locus_tag="U4B03_000179"
     CDS             21694..22830
                     /locus_tag="U4B03_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2067059.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(22881..23084)
                     /locus_tag="U4B03_000180"
     CDS             complement(22881..23084)
                     /locus_tag="U4B03_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2067060.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(23100..24932)
                     /locus_tag="U4B03_000181"
     CDS             complement(23100..24932)
                     /locus_tag="U4B03_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2067061.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            complement(24935..25381)
                     /locus_tag="U4B03_000182"
     CDS             complement(24935..25381)
                     /locus_tag="U4B03_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067062.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            complement(25374..26324)
                     /locus_tag="U4B03_000183"
     CDS             complement(25374..26324)
                     /locus_tag="U4B03_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067063.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            26719..27336
                     /locus_tag="U4B03_000184"
     CDS             26719..27336
                     /locus_tag="U4B03_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067064.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            27433..27639
                     /locus_tag="U4B03_000185"
     CDS             27433..27639
                     /locus_tag="U4B03_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067065.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            27644..27958
                     /locus_tag="U4B03_000186"
     CDS             27644..27958
                     /locus_tag="U4B03_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HEN2067066.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            27959..29101
                     /locus_tag="U4B03_000187"
     CDS             27959..29101
                     /locus_tag="U4B03_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEN2067067.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            29103..29645
                     /locus_tag="U4B03_000188"
     CDS             29103..29645
                     /locus_tag="U4B03_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEN2067068.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            29706..31643
                     /locus_tag="U4B03_000189"
     CDS             29706..31643
                     /locus_tag="U4B03_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEN2067069.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            31854..32624
                     /locus_tag="U4B03_000190"
     CDS             31854..32624
                     /locus_tag="U4B03_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEN2067070.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            32617..32961
                     /locus_tag="U4B03_000191"
     CDS             32617..32961
                     /locus_tag="U4B03_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HEN2067071.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            32954..35197
                     /locus_tag="U4B03_000192"
     CDS             32954..35197
                     /locus_tag="U4B03_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN2067072.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            35335..35544
                     /locus_tag="U4B03_000193"
     CDS             35335..35544
                     /locus_tag="U4B03_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067073.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            35541..35825
                     /locus_tag="U4B03_000194"
     CDS             35541..35825
                     /locus_tag="U4B03_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEN2067074.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            35825..37177
                     /locus_tag="U4B03_000195"
     CDS             35825..37177
                     /locus_tag="U4B03_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2067075.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            37174..37602
                     /locus_tag="U4B03_000196"
     CDS             37174..37602
                     /locus_tag="U4B03_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEN2067076.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            37776..38135
                     /locus_tag="U4B03_000197"
     CDS             37776..38135
                     /locus_tag="U4B03_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2067077.1"
                     /translation="MKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAINKR
                     YGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCKSC
                     HSKIHMTRRNTKDKTIM"
     gene            38269..38841
                     /locus_tag="U4B03_000198"
     CDS             38269..38841
                     /locus_tag="U4B03_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN2067078.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            38856..39638
                     /locus_tag="U4B03_000199"
     CDS             38856..39638
                     /locus_tag="U4B03_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEN2067079.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            39638..40873
                     /locus_tag="U4B03_000200"
     CDS             39638..40873
                     /locus_tag="U4B03_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEN2067080.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            40947..41324
                     /locus_tag="U4B03_000201"
     CDS             40947..41324
                     /locus_tag="U4B03_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HEN2067081.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            41326..41520
                     /locus_tag="U4B03_000202"
     CDS             41326..41520
                     /locus_tag="U4B03_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067082.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            41504..41680
                     /locus_tag="U4B03_000203"
     CDS             41504..41680
                     /locus_tag="U4B03_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HEN2067083.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            41677..41820
                     /locus_tag="U4B03_000204"
     CDS             41677..41820
                     /locus_tag="U4B03_000204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067084.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            41903..43468
                     /locus_tag="U4B03_000205"
     CDS             41903..43468
                     /locus_tag="U4B03_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2067085.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            43524..44258
                     /locus_tag="U4B03_000206"
     CDS             43524..44258
                     /locus_tag="U4B03_000206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067086.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            44324..45652
                     /locus_tag="U4B03_000207"
     CDS             44324..45652
                     /locus_tag="U4B03_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2067087.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            45552..46307
                     /locus_tag="U4B03_000208"
     CDS             45552..46307
                     /locus_tag="U4B03_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN2067088.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            46311..47522
                     /locus_tag="U4B03_000209"
     CDS             46311..47522
                     /locus_tag="U4B03_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2067089.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            47533..47844
                     /locus_tag="U4B03_000210"
     CDS             47533..47844
                     /locus_tag="U4B03_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN2067090.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            47841..48179
                     /locus_tag="U4B03_000211"
     CDS             47841..48179
                     /locus_tag="U4B03_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2067091.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            48172..48603
                     /locus_tag="U4B03_000212"
     CDS             48172..48603
                     /locus_tag="U4B03_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN2067092.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            48600..48941
                     /locus_tag="U4B03_000213"
     CDS             48600..48941
                     /locus_tag="U4B03_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067093.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            48944..49534
                     /locus_tag="U4B03_000214"
     CDS             48944..49534
                     /locus_tag="U4B03_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2067094.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            49544..49924
                     /locus_tag="U4B03_000215"
     CDS             49544..49924
                     /locus_tag="U4B03_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067095.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            50131..52692
                     /locus_tag="U4B03_000216"
     CDS             50131..52692
                     /locus_tag="U4B03_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2067096.1"
                     /translation="MADNFGLKIGVEGEKEFKNSLREINQSFKVLGSEMKLVSSEFDK
                     NDKSISSLSARNQVLNKSIDAQKEKITTLENALKNASDSFGENDKRTQNWTVQLNNAK
                     AQLNNMEREVANNEKAIDAMGTEEVDAAKKTDELGNELKDTGDEAEKSGSKFEKFGGV
                     LKGIGVAMGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTVQKNADNA
                     FKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKMGTDIESI
                     QNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFADVTEAIHV
                     IQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNVVEQFQNV
                     IKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGLLPTLIPV
                     VVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIITIVNGLIE
                     NLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQTGIQLLT
                     SLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALPQIITTIA
                     NAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQIITGLANAF
                     GEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIHSPSTLFR
                     DEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDSTFSMLDL
                     GAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRRRVAY"
     gene            52763..53164
                     /locus_tag="U4B03_000217"
     CDS             52763..53164
                     /locus_tag="U4B03_000217"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067097.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            53241..53933
                     /locus_tag="U4B03_000218"
     CDS             53241..53933
                     /locus_tag="U4B03_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2067098.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            53930..57820
                     /locus_tag="U4B03_000219"
     CDS             53930..57820
                     /locus_tag="U4B03_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2067099.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            57830..57997
                     /locus_tag="U4B03_000220"
     CDS             57830..57997
                     /locus_tag="U4B03_000220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067100.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            57984..58391
                     /locus_tag="U4B03_000221"
     CDS             57984..58391
                     /locus_tag="U4B03_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEN2067101.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            58392..58811
                     /locus_tag="U4B03_000222"
     CDS             58392..58811
                     /locus_tag="U4B03_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN2067102.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            58801..59709
                     /locus_tag="U4B03_000223"
     CDS             58801..59709
                     /locus_tag="U4B03_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2067103.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            59825..60862
                     /locus_tag="U4B03_000224"
     CDS             59825..60862
                     /locus_tag="U4B03_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEN2067104.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(60923..61213)
                     /locus_tag="U4B03_000225"
     CDS             complement(60923..61213)
                     /locus_tag="U4B03_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067105.1"
                     /translation="MEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIEAEEAL
                     YQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            61523..61696
                     /locus_tag="U4B03_000226"
     CDS             61523..61696
                     /locus_tag="U4B03_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067106.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            61757..63310
                     /locus_tag="U4B03_000227"
     CDS             61757..63310
                     /locus_tag="U4B03_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2067107.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            63274..63723
                     /locus_tag="U4B03_000228"
     CDS             63274..63723
                     /locus_tag="U4B03_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067108.1"
                     /translation="MRTTFEREVTRMNNTIPYGYVIKNGEINVDEQQAQQIREFFNIY
                     LEGVGLQEAGKRSGINKPHPFMKRILMNEVYLGNEIYPKIIDEDIFFQADLEQERRIK
                     FFKKRKEIKVPDTKVIPTHFKSGKITEKYSDPFKQAQYAYSKIKEVK"
     gene            63725..65275
                     /locus_tag="U4B03_000229"
     CDS             63725..65275
                     /locus_tag="U4B03_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2067109.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            65398..69921
                     /locus_tag="U4B03_000230"
     CDS             65398..69921
                     /locus_tag="U4B03_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEN2067110.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVGFKKFIYNKYKNEIKFTN
                     INYLTSKYISNFEELIDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGLYF
                     NSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIV
                     EQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGLSV
                     LAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLKLE
                     TFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQ
                     SIVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLNLIE
                     LLISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYPTIF
                     EIDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKKAE
                     HYRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFPEL
                     SVSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLREDI
                     LIFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYDNRN
                     SANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDPEN
                     GNMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQAS
                     SYLFKFKQKRSDG"
     gene            70056..70184
                     /locus_tag="U4B03_000231"
     CDS             70056..70184
                     /locus_tag="U4B03_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN2067111.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT        22748 a        12521 c        15770 g        19203 t
ORIGIN      
        1 aaatatgcta ttatttgtat tgaggtgatc gtgaaaatgc ttacagagga acgtagacaa
       61 aaaatcctag acttgcttga acagcagaaa atcgtgaaat cacaagatct tgtaaacctt
      121 tttaatgctt ctgaatcaac gataagacgt gatttacaag agctggagaa tagtgggttt
      181 ctagaaagag ttcatggcgg agctaagaaa gagcaattat tgaattttga gcagaatatg
      241 agtgaaaaat cactcaaaaa cgttcacgaa aaacagcaaa tcgccaaatc ggcagctgaa
      301 accattaatg acgaagatgt catttattta gacgctggct ctactacgtt agaaatgatt
      361 ccttttctgc aaggaaaaca gatcacagta gtgacaaatt ccgttcaaca tgcggcaaat
      421 ttagttgatt tggagcttgc taccattatt ttaggtggaa cgataaagct ggcaactaat
      481 gctgttttag gatctaatgc gatagaacaa ctaaaaaatt ttcattttaa taaagcattt
      541 atgggaatga acggtgccca tcttgaacaa ggattcacga ctccggaccc agaagaggca
      601 gctgttaaac gtttagccat gaaaaattcc gaagagacat ttgtattgat tgatcatacc
      661 aaattcaacc agttgtcctt cacctctgtt gcacctttgg aagcagccac gatcataacc
      721 gagcgttgtc cattagaatt cgttaatgaa ttccgtgaaa agacaacaat taaggaggca
      781 atacaatgat ttacacagta acacttaatc catcaattga ttatatcgta cgcgtagatg
      841 ggttaaaact aggcgatttg aaccgaatga aggaagactt caagttacct ggaggaaaag
      901 ggatcaatgt ttcccgtatt ttgaaacgga tcgattctca atctacagca ctagggtttt
      961 taggagggtt cactggcgaa tttatttcga agtggcttga aaaagaacaa atccagtctt
     1021 tgttcacaac cgtaaaagaa gatacaagaa tcaatatcaa actaaaatca gatactgaaa
     1081 ctgaaattaa cggtttgggc ccatctattt cagatgaaga aatcgatgag ttgaagcaag
     1141 cattaggaac agtaggacaa ggagatatcg tcgtattgtc aggaagcgcg ccggctagcc
     1201 tgcgaaaagg attttatcaa gaactgattg atatcattcg agaaaaaggt gcagatttcg
     1261 tgattgatac aacaggtgat gacctaaaaa atgcacttgt taagaaacca ttgttgatca
     1321 aaccaaacaa tcacgaatta gctgagcttt acaatacaac cttccaatct gtagaagaaa
     1381 tcattccgta tggtaaaaaa ttgctagctg aaggggcaca gaatgtgctg atctcaatgg
     1441 ctggagacgg tgctttgctc ttcacggaac aaggaactta taaaacgaat gtattagttc
     1501 gcccattgaa aaattcagta ggtgcagggg attcaatgat tgccggcttt attggggctt
     1561 acagccgtac gaaagatcca atcgaagcct ttaaatgggg agttgcttgc ggaagcgcaa
     1621 cagctttttc tgatgacttg gcagctcgcg aattgattga tgagttgctg ccagaagttg
     1681 aaatcacaaa aattgattag tttcaaggag gataatcatg aatatcaagg atttaatggt
     1741 caaagaagcg atgatcatgg atttgcaagc gactgataaa aaaggcgcaa tcgatgaaat
     1801 ggtccaaaaa atgtttgatg ccggccggat ttccgatatc gaaacatata aagaaggaat
     1861 tctagcccgc gaagcacaaa cttctacagg tcttggtgat ggaatcgcca tgcctcacgc
     1921 gaaaaacagt gcagttaaag aagcaacagt tttattcgca aaaagccaaa aaggagtaga
     1981 ttatgaagcc ttagacggac agccaactta tctgtttttc atgatcgcag ctccagaagg
     2041 cgcaaacgac acacatttgc aagcattagc agcactatct cgattgctga ttgatgcaga
     2101 tttcgtagga aaattaaaag aagcaaatac accagaagaa gtccaagact tgttccaaac
     2161 agctgaagag caaaaagaag cagaagaaaa agcagaacaa gaaacacaag ctgcagaagc
     2221 ttctgctcat ccagaaagaa aattcgttgt tgccgttaca gcttgtccaa cagggatcgc
     2281 tcacacttac atggcagaag atgctctgaa gaaaaaagcg aaagaaatgg gtgtggagat
     2341 caaagtcgaa acgaacggtt cagaaggaat caaaaaccga ttgactgctg aagacatcgc
     2401 ccgtgcagaa ggtgtaatcg ttgcagcaga caaaaaagta gagatgaatc gctttgacgg
     2461 aaaagaactg atcaatcgtc ctgtcagtga tggtatccgt aaaccagaag agctgatcaa
     2521 tctagccctt agtggtactg caccagtatt ccatggtgat ggaaaagaaa gcagttcaga
     2581 agaaggaaat gcggatggta ctatcggaca aagaatttac aaagatttga tgaacggcgt
     2641 atcacacatg ttacctttcg ttattggtgg aggaatcgct attgcgcttt ctttcatgat
     2701 cgaccaattt attggcgtgc cacatgatca attatcaaat ctagggaatt acaatcaagc
     2761 tgctagctgg ttcaaccaaa tcggacaagc tgcatttggt ttcatgctgc cggtattagc
     2821 aggatttatt gcttcaagta tcggtgaccg ccctggtttg atcgttggat ttgctgctgg
     2881 tgcattagct aatacaggcg gtgcaggatt cttaggtgca ctgattggtg gattcttagc
     2941 tggttatgtc atcgtatttt tacgtaaatt attcaagaac ttaccaaaat cattggatgg
     3001 aattaaaacg atcctctttt atccagtgtt tggcttactg attactggtt tcttgatgtt
     3061 gctggtcaat gtccctatga aagcaatcaa tgatgcattg aacggtttct tgacaagtat
     3121 gagtggttcg aatgctgctt tacttggtgc attattagct ggaatgatgg cggcagattt
     3181 aggcggtcca atcaacaaag ccgcttatgt gtttggtaca gcaactttag ctacgactgt
     3241 tgcaactggc ggaagtgtcg tgatggcttc tgttatggct ggaggaatgg tacctccatt
     3301 ggcaattttc gttgctacac gtttgtttaa gaataaattc agcaaaacag atcaagatgc
     3361 tggtttgacg aacatcgtga tgggactatc gtttgtcacg gaaggatcga ttccatttgc
     3421 agcagctgac ccgattcgtg ccattccaag ttttattatt ggttctgctt taactggcgg
     3481 attagtcgga gctttcggga tcaaattact ggctcctcat ggcgggattt tcgttgtatt
     3541 cttactgagt cacccggtca tgtatttggt gtttattgct atcggagcag tcgtatctgg
     3601 tgtgatttat ggtttattga gaaaagctcc tgcagcagca gaagcataga aataaaaaag
     3661 aagaggctgg tttttcccag tctcttcttt ttttatagaa tcatatagaa tcaagtagtg
     3721 tagatgaata gttgttgtaa aatagttttt ccatgttcgt taaaatgatt gcttcaaatt
     3781 tgtgatagaa gatttagcct cgatttcctg cttgattctt ggcaagtttg cttcatcagt
     3841 caagataacc aaaatatctc ctacattcat gatcgtatca ccgtgagtca agatttcctg
     3901 atttcctctg cgtatagaga ttagcagcat atttttaggc caattgaagt ctcgtaccat
     3961 taatccgtct aaagaactct ctgccgttac tggaaaatca aaagttgtcc ttgcaccttt
     4021 tagtgaggcg gtctgagtat ttccaagtag acgttccaat aatgattcgt aaattggatt
     4081 accgccgaga aggtcattga cgatataagc aacaagagca gccaatccta aaggcatcag
     4141 atgattaaga gagccaacca tttctgtcac taaaataatt gctgtcagcg gtgcttttcc
     4201 aatcgctgtg aaataaccag ccatggcaat aacgacaaaa tccttgatgt agattggatc
     4261 catcccgaaa tcaaccataa tgtttccata aaagctaccg attaaagccc ccaatgtcaa
     4321 tatcggaagg aaaatccccc ctggaagacc accaccgtaa gaaatcatgg aaaaaacgaa
     4381 tcgaagaaca aacaaaccga taatcataat gattggcaag gaagcatgtc caaaagctaa
     4441 aacgatttga ttgcctccgc ctagagtact aggccataaa taaccaattg gcaaaatcag
     4501 caagaaagga acgaacccgt gaaaataagc tggtaaaggc aatcggttgt aaaattttgg
     4561 caaagccagc aagaccttct gataaataaa acctaagatg cctagaagta ctcctaagat
     4621 cactaaggaa ccataatatt tcaacggcaa agaaggaagg tcacctagat ataaaacagg
     4681 tctcaagccg aaaaagtaaa gagagacgaa gttagcagtg attgctgaag caaatgatgt
     4741 cagccaaaca agtggagaaa aggagtgatg gatttcttct aaaacaaaga gtaatcctgc
     4801 gataggagca ttgaatgctg ctgcaagtcc gctacttgca ccgctagaaa ttaaaatctt
     4861 ttcttctgaa gatggactcc gaaacaattg tccagctcct tgtccaatgg atgcgccaag
     4921 ctgtatagaa ggtccttctc tacctagaaa aaggcccgca ccaactgaaa gtacaccgcc
     4981 aatgaacttt ttccataaaa cagagaacca gttcattgat aattccccgc gaagctgtcc
     5041 ctctacttgt ggaatcccgc ttcccttgat atccggttca tttttgacca gataacccac
     5101 gaaaaatgct gcaaggatgc agcaaatagc taggccaatt aagtaaatcg gctgttcccg
     5161 actcatttca tagatacttc gaactaaatc gcttagcttt tcgatagata atcgaaacag
     5221 gctaacgacg attcctgtca gcagtccaac aaatattcct tttagaataa aatatatctg
     5281 agtcttatct aatttcttga tttttccttt tttttccatt caaacgcctc gctttctttt
     5341 aatttagcgc gaaatcaacg aattgacaag gaaaaatcca taaaaagaaa caaatgattt
     5401 tgaactagct agtttaatga aagccaagaa aatgctataa ttaacaagat tgataaaaga
     5461 aaggatgttg ttaatgtcat ccaaagctga attattaaac gggatgaacc cccgtcagaa
     5521 agaagctgtt cttcacactg acggaccatt actgctaatg gcaggtgcag gaagtggaaa
     5581 gacgagagtg ctgacacacc gcattgccta tttgattgag gaaaaagaag tcaatccttg
     5641 gaacatccta gcgattactt ttaccaataa ggctgcaaaa gaaatgaaag aacgtgtcaa
     5701 tgcgattctc gcatctggag gcgaagatgt ctgggtatcg acttttcact cgatgtgtgt
     5761 gcgtatttta agaagagatg ttgattttat cgggtataac cggaacttta caatcatcga
     5821 ttcttctgaa cagctgacct tgatgaaacg gatactaaaa gaactgaaca ttgatccgaa
     5881 aaaatatgat cctcgaagta tattaggaac aatctctcaa gcaaaaaaca gcctgcagac
     5941 ccctcaggat tttgcaaaaa tgcaaggcag ttattacgaa gagattgcgg caaaatgtta
     6001 tgctgcttac caaaaagagc ttcaatataa tcaatgtatg gactttgacg acctgatcat
     6061 gaatacgatt cgattatttg aagaacatcc tgattcactc acatattatc aaaacaaatt
     6121 ccattatatt catgtagatg agtaccaaga tacaaatcac gcacagtata cgctagtcaa
     6181 tttattggca ggtagattta ggaacctgtg cgtcgtggga gatgcagatc aaagtattta
     6241 cggctggcgc ggtgcagata tgcagaacat cttggatttt gaaaaagatt atccagatgc
     6301 tgcagtaatt ttgctagaac aaaactatcg ctccacgaaa aacatcctta gcgcagcaaa
     6361 ccaagtcatt gaaaacaata gcaatcgaaa accaaaaaac ttgtggacag aaaacaaaga
     6421 aggaaacaag atcacttact accgtgcaga taatgaacga gacgaaaccc gttttatcgt
     6481 agatcggatg caagaagaga ttcgaagcaa tcatcggaat tacggcgact ttgccatctt
     6541 gtaccggacg aacgcacaat ctcgtgtaat ggaagaaact ctgttgaaag caaatattcc
     6601 ttataaaatg gttggagggc acaagttcta cgatcgtaaa gaaatcaaag atattttagc
     6661 gtatttgaat gtgctggcaa atcctcaaga ttcaatcagt tttgagcgta tcgttaattc
     6721 gcccaaacga ggcatcggac caggatctat cgaaaaacta cgcagttttg cttctcttca
     6781 tgaatggcca ctgcttgaag cagcgcaaaa tgtggatctg gcaaatatcg gtggaaaagc
     6841 aggacagcaa ttaggtgcat ttggagagat gatccaagaa gtcactcaga tgatcccata
     6901 cttgacagtg acagaattga cgaaagaggt tttggatcgt agcggatatc tagaagactt
     6961 aaaaatccaa aatacattag aagcacaagc acgcatcgaa aacttggagg aatttttaac
     7021 ggtcacacaa gaattcgaca aacaattcga acagcaaaat gaagaagacg ctgatgcacc
     7081 agaagaaaaa ctaacggtct tcttaaatga tctggcactt gtttccgata ttgacaattt
     7141 agaagaagat gcttcccaag tgacattgat gactttgcat gcagccaaag gattagaatt
     7201 cccagttgta ttcctgattg gtctagaaga gggggttttt cctttatctc gagcattgat
     7261 ggaagaaagc gaactggaag aagaacgccg tctcgcatat gtggggatca cgcgagcaga
     7321 agaagcgttg tatttaacaa atgctttctc cagaacatta tatggacgga cgcaatataa
     7381 ccgaccttct cgttttgtag aagaaatcga tcaggagtta ctggaaatcg aaggcatgcg
     7441 tcctacgcct aaaaaaacac ctgtattcgc taaaaaaaca gcatatagct ataaacagcc
     7501 agagacagcg gttgttccct ctaaaagtgc tactggaggg gaaaataaca gctggaaacc
     7561 tggagataaa gtcaaacaca aaaaatgggg tctaggaaca gtcgtccgag taagcgggac
     7621 gtcgaaggat ctagaactag atgtggcttt cccaagtcaa ggagtaaaac gtcttttggc
     7681 tgcctttgcg ccaattgaaa aagcttagta gtggagggta aatgatggaa catgaaatga
     7741 cgattgacga agctgctaaa cgagcagaag aacttcgtac aagactgaac caatggtctc
     7801 gcgaatacta cgtagaagat aaaccaacag tggaagacta tgtttatgat aaagaatacg
     7861 cagaattagt agcaattgag gaacaatatc ctgatttgat cacgtctgat tctcccactc
     7921 aacgagtagg cggaaaagta ttagaaggtt ttgaaaaagt cactcatgat attccgcttt
     7981 acagtttgaa cgacgttttc agcaaagaag agctgatagc ttttgatcaa cgtgtacaaa
     8041 aagcagtggg acgtgtagtt gactattgct gtgaactaaa aatagatgga ctttccgttt
     8101 ctttgcgtta tgaagatgga aatttcgtga gaggtgcgac tcgcggggat ggaactgtag
     8161 gagaaaacat cactgaaaac ctaaaaactg tccggtcggt cccaatcaaa ttaaaagaac
     8221 caatgaacat cgaagtaaga ggcgaatgtt tcatgccaaa acggtcattt gtccaattga
     8281 accaagacag agaagcagag ggaaaagata tctttgcgaa tcctcggaac gccgcagctg
     8341 gttctttgcg tcagttagat tctaaaatca cagcgaaaag aaatctggac acttttttat
     8401 acacagtagc tgatttcggt cctatgcaag caaaaaccca gtatgatgcg ctagaagagc
     8461 tagaaaaaat tggattccac acgaatcggg aaaaacgtct atgtcattcg attgatgaag
     8521 tgtggtcata cattgaagaa tatcatgaca aacgtgtgga tttgccctat gaaatcgatg
     8581 gaatcgtcat caaagtgaat gaattctcgc tacaagatca acttggtttt actgtcaaag
     8641 caccgagatg ggcagcagct tacaagtttc cgccagaaga agttgaaacg ctaatcgaaa
     8701 acatcgaatg gacagtcggt cgaacagggg tagtaacgcc aacagctatc atgacaccag
     8761 tccgagtagc aggaacgaca gtaagccgtg ccagcctgca taacggtgat tatattaagt
     8821 tgaaggatat ccgcttgaaa gatacagttc tgatttataa agcgggagac atcatcccag
     8881 aagtttcgca agtcgtgtta gataaacgac ctaaagacag cgaagaatac caattgccta
     8941 cacattgtcc agtttgtgga agtgaacttg tccatttgga tgaagaagta gccttacggt
     9001 gcatcaatcc taaatgccct gctcaaatga aagaaggttt gaaccacttt gtttcaagaa
     9061 atgcgatgaa tatcgatggg ttggggccgc gcgtactaga acagatgtat gataaaaaac
     9121 tagtagcaga tgttgctgat ctttacaaat tgacagaaga agagttattg acgttggaca
     9181 aaataaaaga aaaatcagca aataatattt tgacagcaat cgacaacagc aaagataatt
     9241 cggtcgagcg gttgattttc gggttaggaa tccgacatgt cggagcgaaa gcagcaaaaa
     9301 ttttagcaga acatttcggt gatctagaaa cattgagtaa aagtgactat gagtcgatta
     9361 ttgcgcttga tacgatcggt gatatcattg ccgacagtgt agtcacttat ttcagtaacg
     9421 aagaagtcca tgaattgatg aatgaattaa aacaagcagg cgtcaatttc gaatacaaag
     9481 ggcttcgaaa tgcccaattg caagaagtcg agtctccttt taaagaaaaa acagtagtcc
     9541 ttactggtaa actaacacga tttactcgtg aagaagcaaa agagacgatc gaaaatcttg
     9601 gcgggaaagt gactggtagc gtatcgaaaa aaacggatat cgtggtagca ggagaagatg
     9661 ctggcagcaa gctgaccaaa gcacaagaat taggaatcga agtttggaca gaagatcaaa
     9721 tggcagatgc attggctaaa agtcgctcag tagaagaatg aaaaatgagt tttttcagac
     9781 aaatttcaat aaacaatttc agacacggca ctcttgtgtt aaaatagaga gcaaagtttt
     9841 taactcgttt agaaagaagg ggtagtatgg cgattagtga agaacaagtg aaacacgttg
     9901 ccaaattagc aaaacttgcg ttcgcagatg aagaacttgc aagttttacc gaccaattag
     9961 gtaaaatcat cgatatggtt gaaatgctgg aagaagtaga tacagaaggt gttccattta
    10021 cgtcgaatgt ggttgaaaca gtaaacgtga tgcgacaaga tcgggcgcaa gctggatgga
    10081 accgcgatga attattgaaa aatgtgccgg aaacagaaga cggctttatc aaagtacccg
    10141 caatcatcga taatggggag gctggcgcat aatgacagaa ttacacaatc gttctctaga
    10201 agaactgcat catatgcttg tatcgaaaga aatcactgta caagatttga caaaagcaac
    10261 atttgaacga atcaaagaaa cagagcctga ggtcgaagca ttcattacat tgaacgaaga
    10321 aaaagcattg gaacaggcaa aagctttaga tgaaaaaggg attgatgaaa gcaacgtcct
    10381 tgctggtatt ccaattggta tcaaggataa tatcgtaaca aaagatatct tgacaaccgc
    10441 tgcatctaag atcctttaca attttgaacc aatttatgat gcaacggtca tggataaaat
    10501 ctatcaagca gatatgatcc ctgttggtaa gctaaacatg gatgaatttg ctatgggtgg
    10561 aagtacagaa acttcttatt tcaagaaaac aaaaaatgct tgggacccaa caaaagtacc
    10621 tggtggctct tctggtggtt ctgcagcagc agttgcagct ggacaaattc cagtttcttt
    10681 aggtagtgat actgggggaa gtatccgcca accagcagct tttaacggca tcgtagggat
    10741 gaaacctact tatggccgtg tctcacgttt tggtttgatt gcctttgctt catctcttga
    10801 ccaaatcgga ccaatgaccc gtacggtgaa agataatgca ttagcattga cagctatcag
    10861 cggttatgac gaaaaagacg gtacttcttc aggcgtttca gttcctgatt ttgcagaggg
    10921 actgacaggc gacatcaaag gaatgaagat tgctttacct aaagaatact taggcgaagg
    10981 tgtggcccct ggcgtaaaag aagctgtatt gaaagcagca gaaacattcc gttcattagg
    11041 tgctgttgtg gaagaagtca gcttgcctca ttcaaaatac ggtgtggctg tttattatat
    11101 cattgcctca tcagaagcaa gttccaactt gcaacgattc gatggtatcc gctacggtta
    11161 tcgctcagaa aatgtgaaat cattagatga tgtatatgtc aactcacgtt cagaaggatt
    11221 cggtgaagaa gtaaaacgcc gtatcatgtt aggtacattc tccttaagtg ctggttatta
    11281 tgacgcttac ttcaaaaaag cgggacaagt ccgtacattg atcaaacgag attttgaaaa
    11341 agtctttgca gattatgact tgatcatcgg gccatcttca ccaacagttg cattcggaat
    11401 cggtgagaac attaacgatc caatcacaat gtacatgagt gacatcttaa cgatccctgt
    11461 caacttggct ggacttccag gtatgtctgt accggcaggt ttatcagaag gattaccagt
    11521 tggattgcag atcatcggta attattttga tgaaaaaacg atgtatcaag cggcatatgc
    11581 gtttgaacaa gcaacagaat tccataaaca acaaccagct attttaggag ggaaagacga
    11641 atgaattttg aaacagtcat tggactagaa gtccacgtcg agttaaaaac caactcaaaa
    11701 attttctctc cagcgccagc tcattttggt gcagaaccaa acagcaatac aaacgtgatc
    11761 gactggggtt atccaggcgt gctgccagta atgaataaag ctgcgctgga attcgggatg
    11821 aaagcagctt tggcattaaa ctgtgaaatc tctaaagata ctcattttga ccggaaaaat
    11881 tacttttacc cagataatcc aaaagcatat caaatttccc aatttgatca accaatcggt
    11941 catgacggat ggatcgaaat cgaagtagaa ggtaaaaaga aaaaaatccg gatcgaacgt
    12001 gtccatttag aagaagatgc tggaaaaaac atgcatggta ttggcggtta ttcttacgtt
    12061 gatttgaacc gacaaggcac gcctttgatc gaaatcgttt ctgaagctga tatgcgttca
    12121 ccagaagaag catacgctta tctagaagca ttgcgatcaa ttatccaatt tacagaagtc
    12181 agcgatgtga aaatggaaga aggctctatg cgctgtgatg ccaatatttc tcttcgccct
    12241 tatggacaag aagagttcgg tacgaaagca gaattgaaaa acttgaactc tttatctttc
    12301 gtgaaaaaag gtttagcttt tgaagaaaaa cgtcaagcaa aagttcttct ttcaggagga
    12361 gaaatccaac aagaaacacg ccgttttgac gaaacaacga ataaaacagt tttgatgcgt
    12421 gtcaaagaag ggtctagtga ttatcgttac ttcccagagc ctgatatccc gaaattcgtg
    12481 atcgatgacg aatggattca aaaagtaaaa gctagtttgc ctgagatgcc agcttctcgt
    12541 cgcgaacgct atatccgtga gtttggactt ccagagtatg acgcaatggt attgacactt
    12601 acaaaagaaa tgtctgattt ttttgaagct gttttggaaa atggtgcaga tgccaagcaa
    12661 gcatctaact ggttgatggg tgaagtttct gcttacttga acagtgagaa actggaatta
    12721 gctgagacaa aattgacacc tgagaactta gcaggaatga ttcgtttgat cgccgatgga
    12781 acgatcagct caaaaatcgc caaaaaagtc ttccgtgaat tgatcacaaa tggcggagaa
    12841 gcccaagaag tagttgaaaa aaatggttgg gtgcaattat ctgatccgaa caaactactg
    12901 ccaatcatta atgaaatttt ggataacaac caacaatctg tcgatgattt caaaaatgga
    12961 aaagatcgtg cggttggctt cctagtcggt caaatcatga aagcaactaa aggacaagca
    13021 aatcctggtg tcgtgaataa gctacttaaa gatgaattag cgaaacgata gaggctgaaa
    13081 atgaaaaaag cacgcgtaat ttataacccc acctcgggaa aagaattatt gaaaaaaaat
    13141 ttggcggata tcctacaggc attagaagaa gcaggatttg aagccagcgc ctatgcaact
    13201 acaccagaac cagattcagc caagaatgaa gcatgtagag tcgcagaact aggttttgat
    13261 ctagtagtgg cagctggtgg cgatggcaca atcaacgaag tggttaatgg aatcgctccg
    13321 cttgaacacc gtcctaaaat ggccatcatc ccagctggta caacgaacga ctatgctcga
    13381 gcattaaaaa ttcctagaga taatattaaa gcagcagcag aagtgataaa aaaacaccaa
    13441 accgtaaaaa tggacatcgg cctttctgat aacagttact tcatcaatat cgctgcaggt
    13501 ggctatttga cagaattgac ttatgaagtt ccatcagaac taaagagtat ttttggttat
    13561 cttgcttatc ttgcgaaagg agcagagatg ctccctcgag taaagccaat caaaatgcgc
    13621 ttgaagtatg atgaagggga atatatcgga aatgcttcga tgtttttcct agggctgacg
    13681 aactctgttg gaggattcga acagatcgcg ccagatgcca aattggatga tgggaagttt
    13741 tctttaatca ttgtgaagac agcaaatatc tttgagatct tgcatattgc agcactcatg
    13801 ctgaatggcg gtcgacatgt cgatgatcca agaataatct ataccaaaac aagcagtttg
    13861 ttcgttgaaa cagaagatga tatcaatcga ccagtaatga tcaacttaga tggtgaatac
    13921 ggcggggaag caccgatgca tttccaaaat cttcatcaac acattgagtt ctttgccaat
    13981 accgatcaga ttcctgatga agcaatcaca ggtacagatg aagaagaatt agaggttgtc
    14041 agcaaagaat tcgtaaaaga agtcgaacgt ttaacggatg aagatatcga cggtgacgga
    14101 aaaatagcag acgaaacacc taatcaaaag aattaatcaa aagccggtgc tgaacgtagt
    14161 aaggtcagtg ccggttttag ttgtgtaatt agctgattta tagtagagtt cagtaggatt
    14221 gaaaaatagg cttaaaggag caaaatatga cagaacagca aatagtaaag aaaaacgaac
    14281 gatatacagt cgaaatcgtc gacttgagtt atgaaggaat gggggtagca aaaatcgatg
    14341 gttatccatt atttatcgaa aatgcactcc ctggagaaga agtagaaata ctagtagtaa
    14401 aagcaggcaa taagtttgga tatggaaaag tcgaaacatt tattacacaa tcacctgatc
    14461 gtcagcctgt agaaaacaat gcattacttc gcataggtat cgcaccttta gcccatttga
    14521 agtatgaaca acagcttgtc ttcaaacaaa aacaagtaga gcatgtaatg gcgaagattg
    14581 ctaagatgcc agaagttcct gtacttccaa cggtcggaat ggcagaacca ttcggctacc
    14641 gtaataaagc acagatccct gttcgccgta tcgatggaca gttagccacc ggtttttaca
    14701 aaaagaatag ccatgatcta gtccctatcg aagattttta tatccaagat cctgtcatcg
    14761 atcaagcaat caaagtagtc agagatatct tgcaacggtt ccaagtacga ggatataatg
    14821 aagaaaaaaa tgaaggacag cttcgtcaca tcattatccg tcgtggtcat tattcccatg
    14881 aaatgatggt cgttttagtt actcgtaaag aaaaattctt taaatccaag gaaattgctg
    14941 aagctattca tgaagaattg ccagaagtgg tttcggtcat ccaaaatatc aatgaagaaa
    15001 aaacaaatgt gattttagga gacaaagaaa aagttttata tggcagagac tatatcgaag
    15061 atcagctatt aggaaaaacc tatcgaatct cagcaaagtc attttatcaa gtaaatactc
    15121 aacaagcaga gtatctgtac caaacagcca tcgagtttgc cgagttagat aaaaaagaca
    15181 ttgtgatcga cgcttattca ggaataggta cgattggctt gtctttagca gacagagtgg
    15241 ataaggtgta tgggatggaa gttattccag aagcaattga agatgcacag ttcaatgctt
    15301 taaataataa aatcgaaaat gttcattatg aagtcggaaa agcagaaaaa gttatgaaaa
    15361 aatggcaaga aaaagggatc aaaccatctg tgatcgtagt tgaccctcca cgaaaaggat
    15421 tagatcatca ttttattgaa tcagcaatcg aaatgtcacc agaacgaatc gtctatatct
    15481 catgtaatcc ggcaactttt gcaagagatg ctaagttatt tgcagaagca ggttatgccg
    15541 tgaagaaagt tcagcctgta gatcttttcc cgcaaaccca tcacatcgag tgtgtaggac
    15601 tgcttacacg tgtggaacgt tgatatgaca aggattagag cgattcggga tggtttccgg
    15661 attgctcttt tttcgtgttg aaatgacgat ttttgggtga gcgtagagtt gatggaaatg
    15721 ctatggagct ttgagttgat aggagttaca ccgtagactt gataggttag atgattttga
    15781 taacggttac gtgatataat atattatcga gttttcaagg gagacaaact tatgaagcaa
    15841 atttttatat tttcagatac acagaaagat aatatttctt attttgaacg cgagccagca
    15901 atcttggttt caatgagtcg tagtgatttg ccgtatttga aggaacagga tttttcaaat
    15961 tatccagctg tttatgtttt gattggtggt aataagcgtt acgtgggtca ggcagcaggg
    16021 caatcaattt cacttcgttt atctcaacac tttctcaagg aatataaagc ttgggttgaa
    16081 tcagttttat tctttacaag agcagatggg aaaatgtcaa aagcagatac agattatctt
    16141 gaaaaacgac ttatccaaga tttccaagaa aaatctgact atgaaatgat gaattcaact
    16201 gctggtaata cttcgtatat tgataaactt caaaaagcaa aatcggatca gctatatgac
    16261 acagtgtttg aaattataga tgaaattgca aatatcgatt tgtttggtac ttccgaagac
    16321 agtgaaaccc aaatttatga atttagttca tctggacaat atgaaattga gtatgatggg
    16381 aaaaagatat cttcgaaatc tgctagaggt ttattggttg attttgtttc tcagctattt
    16441 gcggatggaa actacaacaa tcaactgcaa gaattaattg tcgatgagac gccgtcgttc
    16501 gctataattt taggacgtaa accatccatg tataacggaa gaccaaattc agccagggta
    16561 gctgatggag tatgggttta tgcaaatttc aacaaaaaga atgtcagaat caaaattgat
    16621 aaactagctc gtcaattggg gattccggtt aaaataaagt ggaattaata tttgttatct
    16681 cgcctttagt aaatctatgc tgaaggcttt tattttagaa aaatagtttg cgttcgcgaa
    16741 aatatttcat gtttcgtata ataatgatag ctcatagatg atataattga agataccagt
    16801 agaatagagg tgtagtatgt caatttctta taataaattg tggaagttat tgattgattt
    16861 gaatatgaac aaaactcaac ttcgtgaaat atctggcgtc agtcccaatg taatagcaaa
    16921 actcggtaaa aatgagttgg ttgcaatgga gtcattattg aaaattgctg ctgccttaaa
    16981 tgttgatgtt agtgaaataa ttagttcaga tgttatagat gcggaggatg ttaaataaat
    17041 ggcaaaaaat aaagttttaa acatcactga aattgaagat ggtgtaaaaa aaatactttc
    17101 aaataggggc gatgatttca tcactcaatt tttgtccttg tacgatatcc ccaaaacgtc
    17161 gattacacgt gcaaaatcaa aattcgataa tggtgaacca tttgtgatta agaacaaact
    17221 tcattatgaa gaaattgaag gcaacgttgt cattgcgatt gatgcgattg ctcaatcaat
    17281 caaagagcaa aaatctaaac cacgatatat cttggtaaac gattatttag atattgctgc
    17341 aattgatacg aaaactcatg aaactttgaa tattccaatt gaagagctac cggctaatgc
    17401 tgactttttc ttagcgtgga atggcattga gaaggctgat tatcaagctg aaaatccagc
    17461 tgaccgaaaa gctgctgaac gttttgcaaa attgtatgat gtggtggcta aagataatcc
    17521 tgatgccaac gagcatgcat ttaacttgtt cttgattcga gtgctgttct tgttgtttgc
    17581 agaagataca ggaattatga ataagggttc ctttacaaat gttctaaaaa caagaacggc
    17641 tgaagatggt tcgaatttta acgaagtaat caaagaactc tttgcaatct tggatattaa
    17701 tgagttagct cgcaatggca aggatgagtg gctacttgaa tttccatatg ttaatggaaa
    17761 actgtttggt gagccacatg tggatttgac cttttcaaaa gtttcaagag agctgcttat
    17821 tgaagcaggg gaattattga attggaatga aatcaatcct gatatccttg gcgcaatgat
    17881 tcaatcagtt gcttcagcag aagaccgtca cgttgccggt atgcattata caagcgtgcc
    17941 gaatatcatg aaagtcatca agccgttatt cttagatgat ttgacagagt catttgaaac
    18001 tttaaagctt cgcagtgaag aaaacgagct gaaggacatt actgaaaaaa caagaaacga
    18061 taataagaga acaatcatca atgatctaag tgctttgttg acgcgaattt ctaatattaa
    18121 gtttttggat cccgccagtg gttctggtaa ttttctgatc attgcttata aggaaatccg
    18181 tagacttgaa attaagattt tgcaattgtt gggcgactta caaaataatg attccatgcc
    18241 actatcagtg attcatcttg gtaattttaa tgggattgaa ttggatgatt ttgcgcatga
    18301 agtagcaaga ctttcattat ggattgctga acatcaaatg aataaagagc ttgaagaagc
    18361 aataccaggt accattgctg agttgttacc gttaaaagat gctgggaata tagtgtgtgc
    18421 caattctctt cgaattgatt ggaatgaggt gttgcgactc aaagaaaatc atgaaatata
    18481 tataatggga aatccaccgt atctgggcaa aagtcttcaa aatgctgaac aacgtgaaga
    18541 tatggaaaaa cttgttggcg atattcgaaa atataaattg cttgattata tttttgggtg
    18601 gttttggaaa gctaaacaat atattgcaac atctcatggc tcgttcgcat ttgttactac
    18661 taattcaatt tgtcaaggtg aacaagttag tatcctttgg ccgtaccttt taaaagatga
    18721 ggtaagaatt tcatttgcat atcagagctt taagtggaac aacaatgcaa agaataatgc
    18781 cggtgtgact gttgtcatta ttggtatgac aaataatgtg aatattcatc ctaagttata
    18841 tttagagaat gggtcagtaa tgagtgtttc aaatattaat gcatatttag ctaatggtga
    18901 aaatgtattt gtcaacgaat caagaaatag tatttcaaat ttaccctctt tgaaatttgg
    18961 cagtatggca aatgatggtg gtaatctagt tttttcagtg gcagaatact ggaatttgat
    19021 tcaaaaatat ccagatatta aacatttatt taagaaatat ttaggatcaa caggattaat
    19081 aaatggagac ttaagatata ctttatggtt gaccgaggaa gatgcaacta gatacaaaca
    19141 aaattctctt attaaagaaa caataaaaaa tgtaaaaagt tatagggaaa aaagcaagag
    19201 aaaagaaact cgagcactag ctgagaagcc atggtcattt ggtgaagttc gttatgatga
    19261 aaccagtgag aatagtattc taattcctcg tgtgtcatca gaaaaaagag attatgttcc
    19321 tatgggtata gttgggagcg atactataat ttcagattcg gctatggcaa tctataacgc
    19381 tccaatgtgg ctgcttggtc tgttagaatc ccgaatgcat atggtgtggc tgcgctctgt
    19441 cggtgggaaa ttagaaactc ggtaccgtta ttcagccggg ttagtctata atacttttcc
    19501 gattcaggat ttatccgccc agcgtaaaaa tgaaatgact cgcgtgatga cagaaattct
    19561 tgatttacga gaatacgaag gcggcacgtt agccgatttg tataataaag acagtatgcc
    19621 agaatcatta cgtaagaaac accaggaact ggatgggatt gttgatagag cttatcagca
    19681 acgcccgttt gaatctgacg aagatcgact tggaacatta ctaaaactct atcaggagat
    19741 gacaatcaat gactagtaat ttatttgaaa ttaattttga taaaaatcaa tcaaaaacaa
    19801 caaatgacct cggtatgcgt gaaatgcaag aacgtgttta tgaaaagcgt gcgtcacagt
    19861 atttgttggt aaaatcacca ccagcttcag gaaaatcacg tgcattgatg tttgttggct
    19921 tggataaact ccataatcaa ggaattaaaa aagtggttat tgcagttcct gaacgctcaa
    19981 ttggtaaatc atttaaagat actgaattat cgaagtttgg tttttattgg gattgggaag
    20041 taaagccaag tgacaactta actattgggg gcggtgaaaa atcaaaggtt caacactttg
    20101 ttaaattcat gaactcgact gacccaagtg ataatatcct gattgccaca catgctacgt
    20161 tgcgatttgc ttttgaagag ttggacgatt cattatttga caatacgcta ttagcgattg
    20221 atgagttcca tcatgtgagc cgtgatgata aatctgtgtt aggtaatgcc ttacgtagca
    20281 tcatgtcaaa ctctaatgcg catatcttag caatgactgg ttcatatttc cgtggcgact
    20341 ctgttcagat tctggaacca gccgacgaag ataaatttga taaggtcact tacacttatt
    20401 atgagcaact ggaaggatat aaatatctga aatcctttgc aatggggtat agtttctatc
    20461 gtggaaaata tacagatgct ttggataaag taattgatgt caacaaaaaa tcaattatcc
    20521 atattcctaa tgttaattct ggtgagtcta ctaaagataa atacgcggaa gttgatgaaa
    20581 tacttgattt attgacggac ggtggtaata tcacacagaa cgaggatggt atttatgaaa
    20641 tcgaacgtcc agatggtaaa aaactattag tggccgacct tgtgaatgaa gaaggacgtg
    20701 aaaaagtctc agcatatcta gcaagtatta cggatgacat taaagatctt gataagcttg
    20761 atatcatcat tgcacttgga atggcaaaag aaggcttcga ttggccattt gcggaatatg
    20821 cactgacaat cggttatcgt aactcattaa ctgaagtgat tcaaatcatt ggtcgcgtaa
    20881 ctcgtgacag ccccaataag actcatgcac agtttacaaa tcttattgcg cagccagatg
    20941 cacaggatga cgaagtatta ttcgcggtta acagtattat gaaggcaatt tctgcatctc
    21001 tacttatgga acaagtgttg gcgccagttt acaagtttaa accaaaagat ggtaagccag
    21061 acaaaccagg tgatctttca atcagaggac tgaaacctgc tgatacgacg gaacgtacaa
    21121 agaaaatcat cgaaaatgat atggaagatt tgaaatctag cattttacag agccaagata
    21181 ttcaaaatgc gattgtttca ggtgctgatg cgaagatgat taataagacg ttgattccac
    21241 gtgtcatcat tgatcgttat cctgatttga cgaacgaaga gttagaaacc gttcgtcaac
    21301 atacagttgc cgatataaat attgcggctg gacggacaac aacttcagaa gatggtagcc
    21361 gcgaaattat taagatggcc gatcgtttca ttaacattga tgaattgaat attgatttaa
    21421 tcgatcaagt gaatccgttc caacgtgcgt atgaagtgat tagtcgtgat attgatgcac
    21481 cgactcttcg gtttattcag gattatttgt catcaaataa gtatgatttt actgacgaac
    21541 aattggttgc ggcctaccaa gctgctaaga agttccgtgc agaaaatggt cgaaatcctg
    21601 atcgaaatag taaagatgat ggtgaaaaat acttggcctt tgctttgcta cgacttgctg
    21661 agataaaacg tgaaagagag gcagcgaaag aagatggcga atagtttaga tagcatcttt
    21721 gaagatgacg cttttgatga tttaaccaag caagttgaaa aaaagaaagt tgtaaaacaa
    21781 gacccagaag ttgctaaatt tcaggagatt atagactttg tgaatgaaaa cggacgagag
    21841 ccagaaaaaa ctcctgaatg gtctagtgaa cgcgcattgt gggctcgtct tagcggattt
    21901 cgaggaaaac ccgaaaggtc tgagaaggtt aagaagtatg acactcttgg tttattggag
    21961 aaagaattcg aaccggatgg agagtttctc gctgattcag ttgaaatcga tgacgagttg
    22021 aatttagatg atatcttagg ctcagatgat atgattgatg atttttcaga cttgttggat
    22081 gtttcccgct atcgtagaac tgtgaacgct gcagataaaa ttggccgcag aaaacatgca
    22141 agtcattttg aaaagtataa gagcttgttc gatcaagtgc atgcagatat cgcatctggc
    22201 agacgaaaaa ttatcccgtt tgagcagtat gatattcatg aaaatagatt ctacatccaa
    22261 aacggcgtaa tgatgtatat cgtttccatt tcgaaggata cctttatcgc tgataatggg
    22321 aaaacaaatg ccagaatgca tgttgtttat gagaatggga cagagaataa gaatctgttg
    22381 ttacaatctt tagcttcgtc tctatactcg actgaacgtc atggaaggat ggtgacagag
    22441 ccggtagatg aggaaacact ggctgaaagc tttggagaag agtttacgac aggttatatc
    22501 tacgtgctga agtcattaag cacgaatccg gatattagca agtggaataa cctgtataag
    22561 attggtttca ctcgaaatga tgttgaaagt agaattgcca atgcggaaaa cgacgtgacc
    22621 tacttaaatg caccagtgaa ggtcatgctt tctgctgaag tacaaaatgt caacgctcag
    22681 ctacttgagc gcactctcca tcatgtattc caggacaaac aagttgtctt tcaggatgga
    22741 aacttcaaaa aagctactga atggtatatg gttccattga atgatattga atcgaaaatc
    22801 aatgaagtaa tcgctggatt acaaaaataa atatgaaaat gcactgtact catgaatcga
    22861 atacagtgca tttttgatat ttaatcgcga acgatttcaa caatatccga taaatcacaa
    22921 tcaagtgctt cacagatgcg aagtaagata tctgttgtta cattttcgcc tttatttagt
    22981 tttgagagtg ttgctgaact gatatcagtc atttttctga gttgtgcttt attcagtcct
    23041 ctgtcgatta ggagtttcca aagcggattg tagctaaact tcatgtaatc attcctgttc
    23101 tagttataaa tatcgtatag atatccatag cgtccattgt ttttatcttc agtaaagaca
    23161 atctgattta cttgtttatc atcatagttt agtgctggca agttcgttgt attctcaacg
    23221 atgataagtt gtccttcgtt ttgatgatcc ataaagtatt tgaaaagtcc ttctttcata
    23281 ttgtcatcgc catcttgttt tttgcgttca tctaatccaa gaagagggga atcgataatg
    23341 aacacagaag gtgtgtactt agcgtgatta tacaagagat gtcgatatgc cattaccact
    23401 actgaattga taaacgctgt atatccatgt ccgttttcgg tccattttag atttccattg
    23461 attttgatgt cgaaaatatc cttggcgaaa aatgcgttac gaaattcatt aggtttataa
    23521 tgcatattac gtaacatgga gtccagtaac tcagacattt ctttgtagaa ttctgcatca
    23581 aatttctctt taggtttata aaggatacgt tcgtcttctg gctcattctc gattttttca
    23641 agatcacttt taagcttatt ttgcattttt tccatcgaga ttagctgttg tttaatatcc
    23701 ctgtaagttt cgattttatt gattcgatcc tttaacgtct cagctttcgg ttttagttca
    23761 tcgttgattg ctgcatcaag cgatttcttt cttgttttaa gctcatcaag ctgttccatt
    23821 aactcttgca gttcaacttc ggtatcatga gattcttccg tcaaatctga taattgagag
    23881 agcaatgaat gtaattctga cctagcagta tcgatgtagg ttgtttggac aggtttactt
    23941 agttcgccgt tacagaatgg acagctatgt gaagtatcat gttcattatg atcatttaat
    24001 aatcgctcac catctacgat tagtgttagt cgttcaatat ccgattgata ttgataatgt
    24061 aactgtttgt acctattcaa aagaagttga gtttcatgaa gatactgttc cttttgataa
    24121 atctctttta tcaagatttt gctgctggct aacgcatcat tgatttgctt ttcaattgtt
    24181 tcaagttcag cgaacattcc atcgacaatt tgttgaaaat catcagaggg agcctcaggt
    24241 aattctgatt tagccacttc gtagttttca tataattcag aaaggcttga attgatgtag
    24301 ttaatcaccg agttccttct aattttttta tgttcttttg tttcgttttc ttccgagttc
    24361 gcaaaatctg aaccgtatag cagaagtaac aacgatgata aaaaagctgt gcgttgggtt
    24421 gtttccccag gaaaaagaac agaggcgctg gtattgactc tatcttgtgg aataagtgtg
    24481 ctaggcgcta atgtccgcca agttaatttt tgacgtttga aatcctcatt cttgataatc
    24541 tgataatcat catcgatgcc taaaagttgt aaccaaaaat ggctaattgg atttttccca
    24601 gatcgtgcat tatatttttc ataatctaca tattcacctg aactcgttat tttggatacc
    24661 aaaatggagt tgctatcaaa ttctctagtt aatgaatatt tgccattagt cgattgaata
    24721 atcagttcaa ctttactgta tccggttgca tcggttgaaa agggagcagc gtcttttttc
    24781 gcgccataga gaaaatcgat tgccgtaaca atgctagttt ttccggtatt tgaagggcca
    24841 gaaattatgt ttagtccatt ggtgaacttg acagttgcat cttcacgatc tttgcctttt
    24901 atggtaacac gttcaatata ataattttgc attatcattg ctctccttta aattcctttg
    24961 cgcgttggtt tatttgtcgc agaagctcaa cgtcactttt atcttgaatg tatttaacag
    25021 attcgtcagc tatttgtaag tatttttctg catataccga tacgaattgt gatatcaact
    25081 ttaggccatc ttctgataaa gcatacttaa aaccttcttt gctttgcgat ggttggataa
    25141 catgttgcag tacaagattt ttgactgcat ctttaactaa ttcacgtcgc gcaataaatt
    25201 cactgaatct atagctgtta tcaccattta agttgtaatc ggaaatttca aagtcgcgac
    25261 catacgtggt gatgaagtct gtgattaaaa ttctatcagc gctgacaggc tctttaaatt
    25321 cagagagcag taacattact cgaaccgaaa tttcaaatgg agtattaaac aaactattca
    25381 tcttcagtca cccatttgat ctttccgtca ttaaccagca tgtggcaaat gcctttttta
    25441 gcagaatttt gcagcaatcc cggtataaac gataagattg atccgtttaa tgttataaca
    25501 gacgactgct gtagaacggc tttgagtctc tcaacgccat catcgtagtc ttcttcatag
    25561 acttcctcga tgccgtcgta catatcttct tttaaagctt caaagtgctc agttccttcc
    25621 tcttgtgtaa agttatcacg gaccccacga cgtatgcttt cagctttata gtaatttctt
    25681 cgctgtctct tgaaatcctt tagccgcgaa tttggcaggt cgtttgtatg ctcgtatact
    25741 tcgtatccat cggcatctgc atatgctgaa aaaagagcta tgacatacgt catctcctca
    25801 gctctaatat caaggggaac tggcaaatcg tcaaagaatt ttttcacttc atcattataa
    25861 gagaaatcac cattagtgtt gattcttaaa cttgcgagct ctaattcgct aagcttcggc
    25921 ttggcctttt ttgtttttac ttttgtttga gaagcaattg aggtgatttc atcaagtaaa
    25981 agatgggcca ctatttcaga aacgtctgta tctttgggga ttaccacacc tttatcaatc
    26041 aattgttgct taaggacggt tggagcatca tctataagac gttcataaat aaaagtcgtg
    26101 catttgtcga tgttgaattt tccagcaacc cgaactgcat actcttgtgt taatggtcga
    26161 gtaccattgt aaaatcttcg taggttatca tcagaaaaac cgaaaatggt ggcctcgtca
    26221 tcatttgaaa cactatccag taaagcaatt aaaaaatcct gtggcttcac gtactcgctt
    26281 cccataaacg gataaagcgt ttgagtaatt tcacttattt ccaaaataca acctcctttg
    26341 ttacgtccgc cgttgaccgc cgtttgccgc cgatgactga cgaaaacaag ggaggccttt
    26401 ttctataatt aaaacatagt agtttaacta tttaatttta tcatttatgg ttcataaaca
    26461 ctacattatg tttcaagaaa gtgaacgaat ttatcttaaa agcgaatagt cctactaata
    26521 aaatataaca atgcctgagt tgcaataggg caaggttaca aatactgaaa agaactggtt
    26581 atccagaaaa tttttcagat attgtatgcc ttactttatt gcgctctttt ttcgtgatga
    26641 gggacggtgt acgtattggc acggtccctg tttgttttcc ttgctcaaaa gctctttcag
    26701 gcaagaaaga gggatctaat gagtagattt atgaaaacac accaaaacga ccgcagcact
    26761 tacaagtacg tgtttgagcg tactgatgcc aatggacgta tccaaaaaga agtggttgag
    26821 attcgcccag gcgaagacgg cgtcactgag cttaacattc aggagctgca tcgcgctgac
    26881 gaccgtgcga tttacaactt ttataaaaac gcacgtcctg aacgcacaga agaagaaaaa
    26941 gaaaagatca aggcttggaa agagaagttc cgtgaagact ttaataatga gcacggctat
    27001 tatccaatgg acgaatacgt ggatggtgaa gccaacgatt acttcaaacg taactacaat
    27061 ttgtcgctca atgaatttga tgatgaatgc gatgaggggg acaaccccat ccaatcattg
    27121 gtttacgatg cttaccaaga taatgaagaa gatcctcgta tcgatcgttt gcgtgaagtc
    27181 gtgacgcaat tattaccgta ccaccaagaa atctatcaca tgctcgtcac tgaaaacatg
    27241 tctcagactg aagtggcgga caagcttgac aagaaaaaac agtcaatcaa tcgcaccttt
    27301 ggaatcatca aagacaagat caaagaatta ttttagaaaa aagttacggg ggagtttact
    27361 ttccccgttt tccttcgcct gtattgtgta agggagtaga tcctaaacaa ataaagatga
    27421 aggaggacca tcatgaacct aaaacacaaa atccgagtaa acgtgactga tgacacgggt
    27481 aacaaagaaa ctgtcctaca aggtggtgtg cgtcatttgc ctcgctggtt tgcacggtgg
    27541 ctgttcggta agggcaacat ggacaaaatt ctcgtgctca caccagggga cacaatcgaa
    27601 tcagtcgaaa tccgtgaagt taacaagaag ggagcgtaat gacgtgagta aaacaaaact
    27661 atttcttcag gtcgttgagg acgtccaagc gttagctgat agcttacaag agttggctgt
    27721 ggcgatgaaa accaatgaac ctgaaccaca agttgaaaca ccagaaccga caatggaaac
    27781 aaaatcagaa gtcaaacaac cgacgcttga agaagtccgt ggtttgttgg cacgtaaatc
    27841 tcaagaaggt aagtcaactg aagtgaaagc actgattgag aaatatggtg ccagtcgctt
    27901 gagtgatgtg ccgacagaaa actacgcggc tttggttgca gatgcggagg gattgtagat
    27961 gccaacacaa cacgcagtcc tatccgcatc ttcaagtaat cggtggattc actgtccgcc
    28021 aagtgttcgc ttgtcagagc actttgaaaa caagtcgagc ccgtatgccc aacaaggcac
    28081 tgatgctcat accttatgtg agtacaaact gaataagttc attggtaatg acgtcacaga
    28141 cccaacatct acgcttgaat tctacgacga ggagatggag cagtgtgctg aaagttatgc
    28201 gaccttcgtc atggaagaag tcgctaaggc gaaacagaca acagttgact ctattgtaat
    28261 agtagaacag cggctcgact tctcaaggta cgtcccagac ggctttggga ccggcgactg
    28321 tctcgtcatt gcggatggaa ccctatctgt tattgacatg aaatacggtc taggaatttt
    28381 ggtagatgcg tatcagaacc cacagatgat gtgttatgcc ttgggagcgt tggagctttt
    28441 cgatgttatt tatgacattc aagaagtcaa gatgaccatt ttccagccaa ggcgtgagaa
    28501 tgtgagtacg tacacactgc tgaaagaaga attacttcag tgggcaaaag atgtgttgtc
    28561 gcctgccgca gatttagctt tcaaaggtga gggtgagtac caatgcggga aatggtgtgg
    28621 attctgtccg gcgaagaata gctgtcgtgc acgagcagac cataacttga aactggcgca
    28681 atatgagttc aaaccaccag agcttcttag tgatgatgaa atcgaagaaa tcatcggtaa
    28741 agtggatgac ctggtttctt ggagtaacga tatcaaggac tgtgctttga aacttgcact
    28801 cggtggcaaa caatggactc atcacaaatt agtcgagggt cgctctactc gtaagtattc
    28861 taatgacaat gatgtcgctg cggcggtcat caaggctgga tacgacccat atgataaaaa
    28921 gttacttggt gtcacagcaa tgacgaaggc actcggtaaa gagaaattca atgaaatctt
    28981 gggcgaatac atcatcaaac caaaaggcaa gctgacgcta gtcgatggtt cagataaacg
    29041 acaagcggtc acagtaaaca acttaaacga agaatttaaa ccattaacgg aggaacaata
    29101 atatgtctaa aacaagcaaa gtaatcactg gtacaaacac acgtctctca tacttccacg
    29161 gttgggaacc agcatccatc aacgggggcg cagagaaata ttcaacagca gtcttgattc
    29221 caaaatcaga taagagaacg attgatacca ttgaaaaagc aatcgatgca gcaatcgaag
    29281 agggacttgc gaaattcggt gggaagaaac caaacaaagc agaaatcaag ctgccattac
    29341 gtgatgggga tatcgaacgc gacgatgaag cataccaagg ccattacttc attaatgcga
    29401 actcaaccac tgcaccacaa atcgtggatc agcacgttca accaatcttg gatcgttcac
    29461 aagtgtatag tggttgctac gctcgtgtgt cgattaattt ctatgccttt aacgcaaatg
    29521 gaaatcgcgg ggtagcggca gggcttggga atatccagtt tgtacgtgac ggcgaaccac
    29581 tcggctcaca agtatctgca agcgatgagt ttgaaacctt ggcggatgat gatttcttgg
    29641 cttaatcatt aaaacaaaaa cggccatggc agaagtgctg tcgcctgttt gattggagga
    29701 agaaaatgaa acatttgtca ttggatttgg aaacgtatag tagtgtcgat ttatcgaaat
    29761 gtggtgtcta caaatattca gagtcccccg attttgaaat actgctattt gcatacagtg
    29821 tagatggtgg tcccgtcgaa gtggttgatc tgaagatgga cgaaactatc ccggctgaaa
    29881 tattaacttc gctatcagat gaaacagtga ttaaacacgc cttcaatgcg agttttgaac
    29941 gtgtgtgttt gtcacgcttt ctggggttgg atggcttttt gaatccacga gggtggcaat
    30001 gcacaatggt gtggggtgcg acattaggtt tgccgttgtc acttgtagga gttggtgctg
    30061 ttcttggttt ggataaacaa aagctcacag aaggaaaaga cctcatccga tacttttgcg
    30121 tgccatgcaa tccgaccaaa gttaatggtg gacgcacgag gaacttacca caccatgcgc
    30181 caactaaatg gtcagcattt aagacataca atagtcgtga tgtggaagtt gaaatagcta
    30241 tccaaggaaa actttccaat tttccagtgc ttgattctgt ttggaaagaa tactggctgg
    30301 atcaggaaat caacgaccga ggaattgaga ttgataagga ctttgtggaa gctgccgttc
    30361 agttggatga attatcaaaa gctggattga atcaacaatt gcaagaatta actggactag
    30421 aaaatccaaa cagcgtgctt caaatgcggg gatggttact agaccacgga cttgaaaccg
    30481 agtcactcgg taaaaaggca gtggccgaac tactcgaaca acctattgat gaaaatttaa
    30541 agcaagtcct ggtcttacgt cagcaactcg ccaagtcatc cgtgaaaaag tatcaagcca
    30601 tgcaaaactt tgcgtgttct gataatcgtt ctcgtggttt attccaattc tatggagcca
    30661 accgtaccgg acgctttgca ggacgtggca tccagcttca aaacttgcct cagaaccata
    30721 tgtcagattt atctgaagcg cgtagtttag taaaacaaag caattttgaa atgcttaaca
    30781 ttctctacgg caatgtcccg aacgtactct cagaactcct ccggacagcg tttgtaccac
    30841 gtgaagggat gaaatttatc gtatctgact tctcagcgat tgaagcacgt gtgattgctt
    30901 ggctgtcggg tgagaagtgg cgtcaagaag tattcaaaaa tggtggcgat atttactgtg
    30961 cgtctgccag tcagatgttc ggtgtgccag ttgaaaagca tggcgtgaat gggcatctac
    31021 gtcaaaaagg gaaaattgct gagcttgctc tagggtacgg aggatcggtg ggtgcattga
    31081 aagcaatgga tgctcttgaa atgggactaa ctgaggatga attacagccg cttgttaatt
    31141 cttggcgaga ctcaaacatt aacataacgg agctttggtg ggctgttgat aaggcagtaa
    31201 agacttgtgt gaaagaacga cggagcacag cgacgcatgg catcaagttt gaatacaagt
    31261 cgggttttct gttcatccaa ctcttgtcag gacgtaaatt agtttatgta aaaccaaaga
    31321 ttggtgagaa caaattcggc ggtgaagctg ttacctatga aggtgtcggt gctaccaaga
    31381 aatgggaacg tcttgaaagc tatggtccca agtttgtcga aaacatcgtg caggcaatag
    31441 ctagagacat tctctgtttc gcaatggaga atcttcgtaa gtatcgcatt gtcgggcacg
    31501 tccacgacga agtgattatc gaagtgccaa tggaatcgaa ggttgaagac atctgtcagt
    31561 tgatgagtca aacgcctaag tgggctgaag gcttgctact gaacgctgat ggatacgagt
    31621 gtgaattcta tcaaaaggat tagtcaatgt ggagtcagaa atggctccat atttttttca
    31681 aattttttta tcaaaaataa ggttcgaatt gggttctttc ttcgcttata gacgaaggca
    31741 aaaaaatcaa aaataaattc gagtcagagt ttactttcga ccattttctt cgcctgtaag
    31801 ttagaagggg caaaatgcct ctcctgaaaa ttctattaca ggaggttcac aagatgaacg
    31861 aattgaaagt ttttgaaaat gcggagtttg gctctgtacg cacggtgatg attggcgatg
    31921 tccctcactt tgttggtaaa gatgtagcat caattcttgg ctacacaaat ccacaaaaag
    31981 cattacgaga tcatgttgat gaggaagata agacggtgaa cgaatcgttc agcgttaatg
    32041 ggacaatggc tatccttatc aacgaaagtg gattgtactc actcatcatt tctagcaaga
    32101 tgccgaacgc gaagaaattc aagcgttggg tgacaaatga ggtcttgccg tctatccgcc
    32161 aacacggtgt gtacgcgaca gaagatttca tcacaaagtc aatcgaagat ccagcatggg
    32221 ccatttcagt gttgaagcag ctacaagaga aaaaagaaat ggtggcgatg caacaacaaa
    32281 tgattttgga gatgaaacca aaagttagct actacgattt gattcttcaa aacaattctg
    32341 tgatgtcgat ttctaaaatc tcgaaggact acggtatgtc accacaggca atgaacaagt
    32401 tgcttcatga actgggaatt cagtacaagc aaggcaagat gtggttgctt tatcagaagt
    32461 atgccaatca aggctacaca caatcaaaaa ctcatgcgat agatgcagag cgcagcgtga
    32521 tgcataccta ctggacgcag aaaggtcgtt tgttcttgta cgacttgctg aaatcgaaga
    32581 agaacatctt accagtgatt gagcgaggtg ataataatgc ctagacgtaa agattacaga
    32641 acatcagtct atgtctgctc gccgttctca ggaaatgttg tcgtcaacca taaaaacact
    32701 aagcgttact gccgtattgc tcttgaccgt ggctacatgc cagttgcggc acatctgatg
    32761 tatccacaat tcatggacga cgcaacggag cgtgatgtgg caatgcacat gtcacttgtt
    32821 ctcatgggca aatgtgagga agtctgggtg gtgggtgaca ccatctccca aggcatggca
    32881 gttgaaattg aacaagcgga gtattggaag aaacatatca ggtacttcga tgatgagatg
    32941 aaggaggtct caaatgctta agatgacgtt attcacatcc aatcaggtgg gtaattccaa
    33001 gaatggattg ttcccaacca agcaagtggt gacagataaa gaaacacttg ttgccacggt
    33061 caagctggat catgtgatgg ctgagtacaa aggcaactat cgcaaaggtg acaacttcat
    33121 ccagtctgat gtgattccac ttgattgcga caatgaccac tcagacaatc cagatgattg
    33181 ggtagcccct ttcgaagtgg cgatgcagtt tcctgatgtg acgtttgcgg tcagctattc
    33241 acgtaacaac atgaaagaca aaggtggcaa atcagcacgt ccacgttttc acgtatattt
    33301 cccgattcct gccacaactg ataaagatga atatgtggaa ctcaagaagc agattcaaag
    33361 tgagttccca tactttgaca acaatgcgat ggatgcggca cgtttgcttt tcggaacacc
    33421 aaactctgaa gttgaaatct atgaaggtag taagttgatc actgattttc tcgaagatga
    33481 tctgtttgct gattgggata atgcgcagtc agagattagc gaaggcagcc gtaattcaag
    33541 catgagtcac atcgcaggaa agctcatcaa acgctacggt gcaacggaag atacgtatca
    33601 caagtatctc gaacaatccc aaaaatgtaa tccgccactg ccggaggagg agcttaaagc
    33661 tatctggaat agcgcggtca actttgggaa gaaagtgaaa caacaagaag gctacattcc
    33721 tccagagcag tacaactctg atttatcact tgagccaacc gactactcgg acttaggtca
    33781 agcaacggtc ttagctcgcg agtatgaatg taagcttcat tactcaccgt caactggtta
    33841 ccttgtctac aacggcagct attgggaaga gtcgaaacca aaagcgcaag gtgtgatcca
    33901 cgcattgaca gaacgtcaac tggaagaatc tgaaaccgaa attgagaagc gtaccaagga
    33961 aatggttagt aatggcgcgt tcgatgtact tgctagtgtt ggtccgaaaa aggcagtcac
    34021 catgttcaac acagcgcaac gtcatagttt tgatttgtac caacacgcac aagagtataa
    34081 gaagtttgct gtcaaacgcc gcgacagcaa gtatctctcg tcagcattaa ccgaggcaaa
    34141 gcccatgctt gaaatcgagc aacggttact agatgtgaac gagttcttgc tgaacacacc
    34201 aacagcaacc tttgatttgc gaacaggcaa atcacaagac cacaactcag aagattacat
    34261 cacgaagcaa acggaatgtg ccccgagtga cgccaatcag cagatttggt tggacgctct
    34321 gaacaccatt ttcgtaggtg accaagagtt gattgattat gtccagatga ttgtaggact
    34381 tgcagctatt ggtaaagttt acgttgaagc gcttatcatc tcgtatggtg aagggcgtaa
    34441 cgggaagtcc acgttctgga atgtcatcag ccgtgtgctc ggtaattact ctggcagcat
    34501 ctcagctgat attcttacaa gtcaaatccg acgcaatgtg aaacctgaac ttgcggaagc
    34561 gaaggggaaa cgcctactca tcgcagccga gcttgaagag gggatgcgac tcaacacatc
    34621 taacattaag cagctctgct cgacggatga aattgcggct gagaagaaat acaaagaccc
    34681 attcaagtat gtcccaacgc atactttggt gctctatacc aaccacctcc caaaagttgg
    34741 agcgattgat aaaggaacct ggcgtcgttt aattgtgatt ccattccttg ctacgattga
    34801 cggcaataag gacatcaaga attatgcgga ctaccttttt gaaaatgccg gcggagccat
    34861 tttgcaatgg atccttgacg gtgcgaaaaa agtcatcgcc gcagactttc atttaacggt
    34921 tccacaagtt gtcgctaatg caatttctga atataagacg gcgaatgatt ggcttggcca
    34981 tttcttagac gagtgctgcg agactgctgt tgactttgaa gaaaaatctg gtgagttgta
    35041 tgcagagtat cgtgcgtttt gtacgcgaac tggtgagtac attcgtagtt cagccgattt
    35101 ttacacggct ctggaggcgg gcggatttaa gcgccggcga actaataaag gaaacaagat
    35161 tgttggtttg caactcaaat ccgagttctt ggaatagttg attttaaagg aaagtgtaac
    35221 ctgtgtaggt cttatataga acttttctta agggataaaa aattaccacc tatataaagt
    35281 tatgtattgc gtattcacgg gttacaccat acttgcagat tggagaatat ctaaatgaaa
    35341 tttaaacagt ggttacaaat tggaatcggt aaacgtgatt tacgatttga tgagttttac
    35401 gagtttgcca tgtcggatgt cacgtatcct tggaagaaat cattcgataa gcaagtcgca
    35461 catctgatga ttcactgcgc agaaaaggaa tacttggaaa tcttgcgtga tgcgtatttc
    35521 gagtatttga cggtgtggct atgagagaaa agcaggttga gcaagcacta gtgaaagccg
    35581 tcaaaagggt aggtggtatt tgcccgaagt ttacatcacc ggggcttgct ggtgtgcctg
    35641 accgattggt acttatgcca aatgggaaac ttggatttgt cgaagtgaag gcgccaggga
    35701 agaagccacg gtctttacag ctttttcgta tgaaacaact gacagattta gggtttcagt
    35761 gtttcgtgct tgatgagatt gatcagatac ctgagttact agaaagaata ggaggtgatg
    35821 tctgatgaag ttcgtaccac ataactacca gaaatatgcg attgatttta tcaaagatca
    35881 taaaactgcc gcccttctat tggacatggg cttgggtaaa acggtgacaa cgctgacagc
    35941 tattaaagac ttgatgcatg atgactttac aatacaacgt gtcttaatta ttgcaccgtt
    36001 gcgcgtgacg caatcaacat ggccaactga aattcaaaag tgggatcatt tgaaagattt
    36061 atcgtattcc gtggttcttg gaacaccaag tcagcgaatg gcagcattac aaaataatgc
    36121 caacatctat ctgatcaatc gtgagaactt ggactggctg attaacaaat cgggttttac
    36181 gtttgatttt gatatggtgg tgattgacga actgtcgtcc ttcaaaaact taaaagctaa
    36241 acggttcact agctttatga aagtcagaca caaggttgac cgaattgttg gattgaccgg
    36301 aacaccttca agtaatggtt tgatggattt gtttgcggag ttcaaggttc tggatatggg
    36361 cgagagattg ggctattata tttcacgtta tcgagataaa tactttctac ctgacaagca
    36421 aaatggcatg cagatttatt cttggaaacc aagggaagat gcagaacaag aaatctacga
    36481 caagatttca gatatcacga tttcaatgaa gtcagtcgat ttcttggata tgccagagct
    36541 tgtgatgaat gaagtgccag ttgatatggg actcaaagaa aagcaaaagt acgatgaaat
    36601 taaggcagac ttggtgctac aattgaaaga agccaccatt gatgcagcca atacagcggt
    36661 gctttcgaat aaactgctac aaatggcgaa tggagcaatc tatgatgagt ttaatgtgag
    36721 tcatcacatc catgaccaga agttagatgc tttggaagat ttgattgaag gtacaaatgg
    36781 caaaccgatt ctgattgcct attggttcca gcatgatctg gagcgtatca aggaacgatt
    36841 caacgttcga cagatcaaaa cgtctcagga cattattgat tggaacgaag gtagcatccc
    36901 catcgctgtc attcatccgg ctagtgcagg acatggattg aatttacaag ctggtggttc
    36961 aacacttgta tggtttggtc ttacgtggag tttggaactt taccaacaaa ccaatgcaag
    37021 gctgtggcga caagggcaaa gtgatactgt ggtcattcat cacattattg cgaaagatac
    37081 gattgatgaa gatgtgatgt tggctttgaa acgaaaagat aaaacacaat catgtttgat
    37141 tgatgcagtg aaagctagat tggagggaag caagtgacac caaaagaata cttgaaacaa
    37201 gcgtaccgtt tgaataaacg aattgacgac cggattaaag agaaggcgga gctctatcaa
    37261 ctggcggtga cagtttcttc cacaacattc gaagaaaaga tttcaagtgg cacacggaat
    37321 actgaggcac catttgttcg taaccttgag aagtgttacc aattggaagc tgaaattgat
    37381 gcagagattg ataagtttgt agacctcaaa cgacaaatgc gtggcgtgat tgatgaactc
    37441 acaaacgtgg acgaaaagat tgtgttaatc aatcgatacc tgttggaaga gagctgggac
    37501 aagattgctg aagatcttca cgctgacgtg cggacagtct ttcgttggca tagcaaagct
    37561 cttgagaact tgaagctacc agatgaaccc atagtcattt gaaatgtgtc agtgaatgtc
    37621 agtagccttt agtgatatca ttaagatgtc aaaaaagaca aacgatactc aagcaatacc
    37681 agcaatagaa gtatcgaaca aatactcgaa gcctttgtgg gaaaaatcct tcaagggctt
    37741 ttcttttgcc cgaaaggatg tgaccccact tgccaatgaa accagctaac ccctgtaagt
    37801 tcccaggttg tccgaaccgt acgcatgata cttattgtga tgaacataaa acacttgcga
    37861 ggaaacgtta cgagaaatac gagcgtgatc cggcaatcaa caaacgttac ggcagagcgt
    37921 ggaagaaaat acgggcaagg tacgtggcag cacatcccct atgtgagatg tgtctgaagg
    37981 aaggacgctt tacttcggtt gagattgtgc atcacatcaa ggaattaagt gatggtggta
    38041 cgcatgatga aaataatttg atgagtgtct gcaaatcatg tcactcgaaa attcacatga
    38101 ctcgacgaaa cacaaaagat aaaacaatca tgtgaaaaat aacaaggggg ggaggggcgg
    38161 taattatccc taaacccttc taaactgtac agcgcgctag gcccttacgc gaaaaaagtt
    38221 cagttcaaac gggggatagc cccaaacaag aaaaagaggt gataaatttt ggcaaaagat
    38281 ggaacacaac gcggtggcag acgacctcgt tcaggggaac gctcacagcc actcgttgat
    38341 aaagttgctg ctggaaagaa agcatcgcgg atggaatttg acttaccgga tgttgaatta
    38401 ttaattggtg aagatattgg tgagggtgta gagcttgagg gcaatgatta tcctgaacca
    38461 agtgcttacc tttctgcaaa acagaaggat ggcactgtgc taggcgctga tgaaatttat
    38521 aactcaacgt tccaatggtt gaaagaccgc ggatgcgaaa cgttgatagc cccacgaatg
    38581 gttgagtcct acgctcagaa ctttgcgcgt tttgttcaat gtgaaaatgc tctgagtcaa
    38641 tatggtttgc tcggaaaaca cccgactacc aaggcagtca tggcctcccc atttgtggcg
    38701 atgtcgcaga acttccaaaa gcaagcaaat gtgacgtggt atgagattca acagattgta
    38761 aaagagaatg catcagtgga atacacagga gatccgaata cggatgcgat ggaacgattg
    38821 ttacgttcta agaatggttg aaaggaacag aatttatgat agagaaggtt aatccgcaac
    38881 atccagataa agttgcagat cgtattgccg gagccattgt ggacttggca tacgcagaag
    38941 aaaataatcc aaagatagca gttgaggttt taattggtca tggcatctgt catgtgatta
    39001 ttgaaacttc aactgcttta aatttgaatg aaatcagcca agcgattaat cgtatcgcag
    39061 gcaaaattaa aaaagatgtg gtgattgtgc cccaagataa agagttggct gacaatcaag
    39121 cgactgaaat tcgttgtggc gataatggaa tctttcgtgg gatgccattg actgatgaac
    39181 agttacagct ctcaaaaatt gcgcgtgact tatatcagga ccatccattt gatggaaagt
    39241 acattcttga tggtgaacgc ttgataattt gtcagagcca cgcaaagaca aatgagttaa
    39301 aacaaaaata tccaaacgct acagtcaacc ctctaggtga ttggactggc ggcccggatg
    39361 tggatacagg tgcgactaat cgcaagcttg gttccgatat ggcagacgga gtaacaggtg
    39421 gcggtatcca tgggaaagac ttatcaaaag ctgatgtgtc agtgaacatc tacgcattct
    39481 tgaaagctca agaaactggt atgcccgttg agttaagctg tgcgattggt gacactgaga
    39541 ttgatggcgt gccttattct gaaatcgtgg aagttgcgcg tgattatgtg aatgagcttg
    39601 gcgggtttga gaaactagca gaatgggggt tgttctaatg aacacaacag aacgttttga
    39661 gaaagttgat attgataagc tggtccctta tgccagaaat gcccgtaccc acagtaagga
    39721 tcaagtgaaa cagattcaag cttctatgcg tgagtttggg ttcataaatc cggtgattgt
    39781 tgataaggat tataacttga ttgcaggtca cggacgtatt cttgcggcta aagaagaagg
    39841 tatcaagcag gtaccgtgcg tgtttgtgga acacctaacc gaggcacaaa agcgtgcgta
    39901 catacttgct gataaccgac tagcaatgaa cgccggttgg gatgatgaaa tgttggcggt
    39961 tgagttatca gacctgcaag gtgaagagtt tgatttggat ttgcttggtt ttgaaactga
    40021 tgaacttgat aaattactca acggtaatga tgatgcccaa gatgatgact ttgatgtaga
    40081 tgaggaactt gaaaagccag ccttcagtaa aacaggggac ttgtggttgc ttggtcgtca
    40141 ccgtttagtt tgtggtgact caacaaaacc tgaaacttat gagaaactca tggacggcaa
    40201 actagcaaat cttgtggtga cagatccacc ttatggggtc tcatatcaag gtaaagcggg
    40261 aacgattaaa aatgataatc tgaaggacga ggcattttac aacttcttgt atgatgcctt
    40321 ttcttgtatg gaaagtgtga tggccaacga cgccagtatt tatgttttcc atgcggatac
    40381 ggaaggcttg aacttcagaa aagcattcgt ggatgcaggg ttctatttat caggtacgtg
    40441 catctggaag aaacagagtc tagtgcttgg tcgttcgcct taccaatggc agcatgagcc
    40501 ggtgctctat ggctggaaga agaaaggcaa acatcaatgg tacaccggac gtaaggaatc
    40561 gaccatctgg gaatttgata aaccaaagaa gaatggtgac cacccaacga tgaaaccaat
    40621 tccgctgttg gcatatccga ttaccaattc gtcactcagc aactgcatcg tgttggatag
    40681 ttttggtggc agcggatcaa ctttgattgc ttgcgaacag accgaccgga tttgccatac
    40741 gatcgaactg gatgaaaagt tctgtgatgt gattgttaag cgttatatcg agcaggctgg
    40801 aagcgatgca gacgtttcag tgattcgaga tggaaagact atcgcattta atgacctaga
    40861 aaatcctgaa taaataacgt atcacttgct ataaaatccc tttagagtga tatatagact
    40921 accaaaagaa tacggaagac aaagttatgt ttccaactca tgaacaaatc gagggtatga
    40981 aaaaacgtta ccctgaaggt agtcgtgtag aactcatcaa gatgaacgac ccacatgccc
    41041 cagcgaaggg cacacaaggc acagtaaccg gtgttgacga cattggttca atccttatcc
    41101 aatgggacaa cggttcaaca ctgaacgcgg tatacggcga agatgtggtg cacatcatca
    41161 aaccagactt cgaattggtc tttcaaaacg gtagaagaga aggatttgaa accttcaatg
    41221 atgcttggca atacgtgacg gacatgatat tgaaccacga tttggtctgg gttgaccttg
    41281 agtcaaaaac agaagatacc atctacatca gaaaggggct ttaagatgaa agtgagtaaa
    41341 cgtaagattt acaacatcgc aaagaattac atcgttcagt tgcctgaacg tggcgacctg
    41401 aaggcccaca acagcgaccg tgaagacttc ttggacattg cggtttggtg tttggaagat
    41461 gccttaattg cggcctatga ggcaggcaga aaggacggtc aaaatgaacg caaagattaa
    41521 gcaacaaatc attgagattg cagaaagtgg tctgacaaat atgtttgatg cgattgcagt
    41581 gcagcgaatt gcacatgacc ggcactttta cgagttggtg attttcattg aagaacacaa
    41641 agatgagtac agccgcttta tcttggaggg tgataaatga tggattatca aatttacatg
    41701 ccagataatg aagatggcat tgaagaagga tactacgatt ggcaagaact ggtgcaattg
    41761 ctgcgggata acaagaacaa ccctgaagcc attcagttca ttgccgatat gatggaataa
    41821 agagattaag gcagctgaca acagctgctt tttgcgtaga agggagtgag ttccaatcga
    41881 taattacaaa ccaactgaat ttatggacaa agactcccat tatgataagt cacgtgcaga
    41941 ttatgccgtg gcctttattg agttgttgaa acacacgaaa ggtcagtggt tcgggaaacc
    42001 gttcaaactg atgccttggc aagaaaagat tatccgtgac ttattcggaa cgattaaggc
    42061 aaatggctac agacaattca atacggctta tgttgaaata cctaagaaga atggtaagag
    42121 tgagttagcg gctgccgtgg ctttgctttt gacttgcggt gatggggaag aacgcgctga
    42181 ggtatatggc tgtgctgctg atcgagcgca ggcttcaatc gtatttgacg tagctgttga
    42241 tatgattcgc cagaacccag cgttaagcaa acgagtgaaa atcctgactt cacaaaaacg
    42301 aattatttat cagccaacga atagtttcta tcaagtatta tcagctgaag catactctaa
    42361 acacggatta aacagttctg cggttgtatt tgatgagctt cacgcacaac ctaacagaaa
    42421 gctgtttgat gtcatgacga aaggggcagg ggatgctaga acgcaaccgc tattcttttt
    42481 gattacaacg gcagggaatg ataccaattc aatttgttat gagcagcatc aaaaagctct
    42541 cgacattatt gaaggtcgaa agcatgataa aacattctat ccgttaattt acggtatcaa
    42601 gcaagatgat gattggactg atccagaagt ttggaagaaa gccaatccgt cacttgggat
    42661 aacgattgca gaagaaaagg tggtggctgc ttgtgattcg gctaaacaaa atccagctga
    42721 agaaaactca ttcagacagc ttcgcctgaa ccaatgggtg aaacaagcag tgcgttggat
    42781 gccaatggat aaatgggacg cttgttcatt caatgcagat gaagaaagtt tgagaggtcg
    42841 agtttgttac ggaggtctcg acctttccag cacgaccgac cttacatcct tcgttttggt
    42901 gtttccacct gaagatgagg atgacaagtt tgtgatactg ccatattact gggtgccaga
    42961 agataccttg gacttacgag ttaaacgcga ccatgtgaat tatgatttgt gggccaagca
    43021 ggggtacata caaaccactg agggaaacgt agtccactat ggtttcattg aaaaatttat
    43081 tgaggaactg ggtgagaaat acaatatccg ggagattgcc tttgaccgtt ggggagcagt
    43141 tcaaatggtt caaaacttag aaggtatggg atttactgta gttcctttcg gtcaaggttt
    43201 caaggatatg agcccggcta ctaaagagtt gatgaagttg actcttgaac agaaattagc
    43261 ccatggcggt catccggttc ttcgttggaa tatggacaac attttcgtcc gaactgaccc
    43321 ggcaggtaat atcaaagctg ataaagaaaa atccactgag aagattgatg gtgccatcgc
    43381 gacaattatg gctctggatc gtgcaattcg ttgtggaaat gataatggtg ccagtgttta
    43441 tgatgatagg ggattgttgt ttctgtgaaa tatgagcttt tattacaatc cattgatata
    43501 attgtctttg gaggtgtatg tgtatgaatg aatatttgat tcaagtagca ccagataaaa
    43561 tatcttcaga cccggacaac acattaattt ctagactgtt ttatagaaaa tggtggggaa
    43621 aaattctaac tgaagatttg acaaagtatg ctgaaggaat tgggaattat aggggagaaa
    43681 cgattatttc tttcaacaca attgctggtg caacgatacg gttattgaaa tgctataatc
    43741 aaaagaactt ttcaatgcca gatagtcatt atgggcgttt gaaagtcatt ttatattccg
    43801 atgatatcac cgaagagatg aaagtgaaat ttgtaaaatt ctatgattta agtcatactc
    43861 ttgcaaattt tatgccacta tatgtatcaa aaccttctct caatggtgtt aaaggtaaag
    43921 ggccgtatca tgactttcct gatttatttt attctgatgt aagagataaa cgatatggtt
    43981 catcaactaa cgctgataaa tttatcgtta ataataaaga ctatttctct aaattcaaag
    44041 attggaaaag ttttatagaa ctaaattatt tacaagattt cttcgatgat gaagattata
    44101 tgacgtttaa aaaacttgca ccaagtagtt cattctttcc ttatcaaaga aaatccttaa
    44161 cacctgacga aaaatcgaaa tgtttagagg aagtaaatac ctttttaaat aatgcaattg
    44221 agataatttt gtcaagagga agtcggtttt atatctaatg tgaacacagc atctacaaat
    44281 cgtaggtgct tttttgatac tcaaattttg aaaggagcaa tcaatgggca taataaaatc
    44341 actattcaaa tcacgcgata aaccaacaaa tcaaaccatc atgaatccat accgatttat
    44401 gtttggcggc tcatcagctg gtaaaagcgt gaccgaacaa acagctatgc agatgacggc
    44461 tgtgtactca tgtgtgcgcg tgctatcaga agccgtggca ggattgccat tacactttta
    44521 ccaataccga gatgctggcg gcaaagataa agcaatgaaa cattcgttgt atttcttgct
    44581 gcatgatgaa ccaaattcgg aaatgacatc atttgtattt cgtgaaacct taatgactca
    44641 cctgctactt tggggtaacg cctatgcgca aattatccga aatggcaaag gtgaagttgt
    44701 agctttgtat cctttaatgc cagataagat gacggttaat cgcgatgaca acaaggaaat
    44761 ctattattta tatcaagctg atgtcgggca agtcagactt tcaaaagctg acgtgcttca
    44821 cattcctggc cttggctttg atggcttggt tggttattca ccaattgcca tggctaagaa
    44881 tgctatcggg cttgcgattg caacagagga atatggttct aaattttttg ccaatggtgc
    44941 caatcctggt ggtgtcctag aacatcctgg aactttgaaa gacccaggaa agcttcgtga
    45001 aagttggaat gcgacatttg gtggcagttc aaatgcggcc aaggttgcag tacttgaaga
    45061 aggcatgaag tatacgccta tttccatgtc accagaacaa gcccagttcc ttgaaacacg
    45121 taagtttcaa ctggaagaaa tcgcacggat attccgagtg ccccttcaca tggttggcga
    45181 cttagaccac gcgaccttct caaacgttga acatttgagt ttggattttg tgaagttctc
    45241 attatcacca tggctcaatc gttgggaaca agcacttaat aaatcactgt taagtcgcga
    45301 tgaaaaagaa aagtatttca taaagtttaa tgtggatggc ttattgcgtg gtgactatgc
    45361 cacacgtcaa caaggctatg caaccggtat tcaaaatggg ttcctatctc ctgatgatgt
    45421 gcgtgaactt gaggacttga acccaatccc tggtggtgct ggtgcgaaat attacttcaa
    45481 tggcaatatg ctgccaattg aacaagccgg ggcagcttat ggcgtaaaca aagaagaaag
    45541 cgaggaacaa gatgaagaag ttttggaagt ggaagaatca aacggttctg aatcaagaca
    45601 accaagagga aatcctagag agaaccctct acctcaacgg accaatcgct gaggagagct
    45661 ggtttgatga tgaagtcaca ccgcagattt tcaaagatga gttaaattca ggtggaggta
    45721 acgttacagt ctggattaat tcgggtggtg gcgattgtgt cgctgccgca agtatttaca
    45781 acatgcttgc tgactacaaa ggtaaagtca cagtcaagat tgacggcatc gccgcttcgg
    45841 ccgcaagtgt tattgcaatg gcaggtgaca aggtactcat gagtcctgtg gcgatggtga
    45901 tgattcacaa tccgtcagcg gttgcgtttg gcgaaaaagt tgatatggag aaagccattg
    45961 ccatgcttga tgaagtgaaa gcaagtatta tcaacgccta tgaacgtaaa acgaatttat
    46021 cgcgtgtgca actggcacat atgatggatg cggagacgtg gatggacgca aataaagctg
    46081 ttcacctagg atttgccgat gaaatcatcg gtgagattga cccggatatt ccggttaata
    46141 attcgatgta ctcggaagtc aaagtaacta attcactgac ttcagcgatt gctaagaagt
    46201 gtcgtattga accacctgaa ccaaaggaac gagaagaaat taataacact atcccagccg
    46261 attcgttaat gagtcggctt aatttaatga aaaattggag gaactaaccc atgaacaaaa
    46321 ttcaagaatt gatggaaaaa cgtgcacaag cttggaacgg agcaaaggct tttgtagaag
    46381 caaagcaaga taaagatggc ttgatgtctg aagaagatgc caagacttat gcggaaatgg
    46441 aaacaaaagt gaagaacttt acgatggaaa ttgaacgtat gcagtcaatg gaagcgatgg
    46501 atcgtgaact tgaaaagcca atgagccaac cattaacttc gcaacctatg actactgaaa
    46561 ataaaatgga acctaagaaa acaggacgtg cttcagctga atataaagaa ggtgtacttg
    46621 aagccttgcg ctcaaacttc cgacatgttt caaatgttct tcaagaaggt gtggatacgg
    46681 caggcggtta tttggtgcca gaagaatatg actcacgttt gattgacggc atgacttctg
    46741 aaaacatcat gcgtacactt gcgacaacaa ttacgacaag tggtgaacac aagattaata
    46801 ttgcaggttc taaaccagcc gcttcatgga ttgaagaagg tggcgagttg agctttgggg
    46861 atgcaacgtt cgatcaaatc ttgcttgatg ctcacaaact tcatgtggca atcaaggtga
    46921 ctgatgaatt gctttatgac aacgccttta accttgaaaa ctatatcatc gaccaattca
    46981 ccaaagcctt agcaaacgcg gaagaagatg cgttcttgaa tggtgacggt gttggtaaac
    47041 cactcggtat ttttgcagca acaggtggcg gacaagttgc cattacaact aatactcaaa
    47101 gtgcagttac ggcagatgat gtggtgaacc ttgtgtatgc tttgaaacgt ccatatcgta
    47161 aaaacgcgaa gtttatcatg aacgaccaaa ccatcagctt gcttcgtaag ttgaaagaca
    47221 acaatggtgc ctacatgtgg cagccagctt tggtagcagg tgaacctgac cgtctgctcg
    47281 gttatccagt ttatacttcg gagtttgttc caaccgttaa ggcaggtgcg ccagtcattg
    47341 cttttggtga tttcagctac tataacattg gcgaccgtgg cgtgcgttca ttcgaccagt
    47401 tgcgtgaact ctttgcaggt aatggcatgg ttggtttctt agctaaagaa cgtgtggacg
    47461 gtaaacttgt gttacctgaa gcagtgcaaa tcttgaagat gaaagcatct gcctcatctt
    47521 aaggagtagc ctatgagtga cttactaaat aaggtcaagg caaacctcat acttgagcat
    47581 aacgaagatg atgagctgtt ggaacgattg attctgaccg ctctttctta tgctgagagc
    47641 tatcaacata ttgagtctgg ctactattca gaacatgaaa tgccgccaac cactgaacag
    47701 gcggttattg tgttagccag taacttttac gaaagccgtg atggttcaac tggtggcttc
    47761 tttgcggata acgtcaatgc cagtaagcaa gtctgggaaa cggtcaatct gttactcagg
    47821 ctcgataggc ggtggcaagt atgagttttg gaaagatgaa tcaacgaata caaatcgtta
    47881 aagtgacgaa ccaaaaggat agcgctggat tttcagtgaa acaagatgaa gttcttgcag
    47941 ctgtccatgc atacaaagag gaaaagaatg cgactgagaa atgggtaaac atggcgactt
    48001 ttagttcagc gacctgcctg tttcggattc gttatattcc taatctagag gttacgacag
    48061 agatggttgt catttcagat cagaaaaggt atcagattgt gtcggtggag aatgtccgaa
    48121 atcgcaatat gtaccttgaa atgctgacta ctgaaatgga ggtgagtgcg gatggctaaa
    48181 gcattgatga agatgccaga agatttcttg ttaaaggttt caaaactaga aaacaagaca
    48241 gatgaaatca tccccaaggt tttagaaagt ggtgctgaag tggtggaagg caaagtccga
    48301 agtaacttaa ctgccgttgt tggctcaaat acaaaagtac caagtcgttc aactggtgaa
    48361 cttgaatcag cccttggtat cacacaagct cgtcaagata aaagcggcaa ctggaacatc
    48421 aaggttggtt ttgatgagcc tcgaatcgat ggcagttcaa atgctaagat tgcgaacatt
    48481 ctcgaatatg gaaaacacgg tcagccagca aagccattct tgaaacctgc aaaatcacaa
    48541 agtcgcaagt catgcattga aacaatgaaa tcaaaaatgg aaagtgaggt aaacggcatt
    48601 tgaacatttt agaggaattg aacagtcttt ttgccacact tcatattcct gttgaaacgg
    48661 gcgagtttag tgataaagcg ccagacaagt attgtgtgct gacaccactc gctgacaggt
    48721 tcgatgttta cggggataac tttccaaccg ttgatgtaca ggaagttcgc atttccttgt
    48781 ttgtgaaagg caactatctg aacacgcaaa aacaactggt tcagaacttg ttgaaacacg
    48841 agtttacgat tactgaacga ttatttgtag gatttgaaaa agatacaaat tactatcatg
    48901 tggcgattga tgtcgccaag cattatgaaa tggaggtctg aaaatggcaa cgattggatt
    48961 ggataaatta tattatgcac cgattacaga gtcggcaact actggcgaag aaacatacgg
    49021 cacacccgct atgcttgcga aagcaatctc agctgagctt tcggtggaac ttgctgaagc
    49081 aactttatac gccgatgatg gtgcatcaga agtagtgaaa gaatttaaaa atggaaagtt
    49141 aacgctcggt gttgatgata ttggacgtgc tgtggcggca gtgcttgttg gtgcaaccgt
    49201 ggatcaaaac ggtgtcttaa tttcgacaac cgaagatggt ggcacaccag ttgcgattgg
    49261 tttccgtgct cgtaaatcga atggcaaata caaatatttg tggttgtacc gagtgaagtt
    49321 tgccgtgccc agcacaagcc tagcaacaaa aggtgacgac attacattct caacaccaac
    49381 aattgagggt acagtcatgc gtcgtaacaa gttagatgct agtggtaagc atccatggaa
    49441 agcagaagtt gatgaaaacg atacgggagt tgaacaaaca actatcaatg gatggtacac
    49501 gagtgtttat gaaccagatt tcacagcaga ctaggaggca acaatgagtg aagaacgcag
    49561 tgccaagatt agccttggcg gagatgaata tgaactgatt ttgacaacaa aagccacgaa
    49621 agaaatcgcc agtcactacg gtgggcttga taaccttggt gagaaattgt tgaaatccga
    49681 gaactttgaa ttggcgcttg atgaaatcat ctggttgatt acgctgttgg cgaatcagtc
    49741 catcaagatt tacaacttga agaacacaga aaagaaagat gaactcacga ctgaatatgt
    49801 ggagttgatg acgagtccgt tgcagttggc tgaatataaa tcagcaatca ctgaagcaat
    49861 gttcaagggc acagctcgca atatcgaaag tgaaacagat tcaaaaaaca aagcaggcga
    49921 gtaagtgatg aagaaacatt tactcgcctg tattactatg ggacagttca aatgggtatg
    49981 acgtcagatg atttctggta ctgctcgctt ggactgtttt tggatttatg ggagtgtcat
    50041 aagcaattta ctgggattag caagccgaaa gttgagttat tcattgatga tgtgataccg
    50101 agtggaattt aagggaaggt ggtgatttat ttggcagata attttggttt aaagattggc
    50161 gttgaaggtg aaaaggagtt taagaactcg ttgcgtgaaa tcaaccaatc attcaaggtg
    50221 ctaggttcag aaatgaaact ggtatcatcc gaatttgata aaaacgataa aagcatcagc
    50281 tcactgtctg ctcgcaacca agtcttaaac aaatcgattg atgcccagaa agaaaaaatc
    50341 accacccttg aaaatgcttt gaagaacgct agtgacagtt ttggtgaaaa cgataagcgt
    50401 actcagaatt ggacagtgca attaaacaat gcaaaagccc aactcaataa tatggagcgt
    50461 gaagttgcta acaacgagaa ggcaattgac gccatgggta ctgaagaagt tgatgctgct
    50521 aagaaaactg atgagcttgg caacgaactc aaggatacag gtgacgaggc tgaaaagtct
    50581 ggaagtaaat ttgaaaagtt cggtggcgtt ctaaaaggta tcggggtagc aatgggtaca
    50641 gtggcacttg ctgctggcgc tgctgccgtg gagctaggaa aacaagtggt atctgcttac
    50701 gcggactatg aacaattagt cggtggggtg gatactcttt tcaaggatgc gtcatccacc
    50761 gttcaaaaga atgccgacaa tgctttcaaa actgccggaa tgtcagccaa tgaatacatg
    50821 gaaactgtca caggtttctc agctgccttg atttcatctc tcggtggtga taccaagaaa
    50881 gccgcaggag ttgcggatca agctgttact gatatgagcg ataatgcaaa caagatgggg
    50941 acggatattg agtccatcca gaacgcctat caaggtttcg ccaagcaaaa ttacgggatg
    51001 ctggacaact tgaaattagg ttttgggggc acgaagtccg aaatggaacg tttgctccaa
    51061 gaggctgaga agatttctgg tgtcaaatat gatatctcga acttcgctga tgtgactgaa
    51121 gcgattcacg ttattcaaac cgaaatgggc atcactggta caaccgccaa ggaagcgagt
    51181 gagacgataa gtggttcgat tgacggtatg aagtctgcaa tctcaaactt gatggctgga
    51241 cttggtaaca gcagcgctga cattggtatg ttgattggca atgtggtgga acaatttcaa
    51301 aacgtcatca agaatattct accggtcatt caaaatatcg tgtcggcttt accggcagtg
    51361 gtggatgcca ttattcaagc attggggcag ttattgccaa cactcttaga agcagtaacc
    51421 tcattattct cacaagtgct tgaaacgata cttggcttac ttcccacact tattccagtg
    51481 gtggttcaag ccgttttaac gattgttcaa acacttattg agaacttacc tttactgatt
    51541 gacgctgcct ttcaacttgt gacaacactt atccaaggac taggcgaagc gctgccagaa
    51601 cttattccaa ttacagtaca ggcaatcata acgattgtga acggcttgat tgaaaacttg
    51661 ccattactgt tggatgctgc tttacaactt atcatgggac ttgcccaagg acttatcaca
    51721 gcacttccaa tactgattga aagtctgcca cagattatta caggaatcgt gaactttatt
    51781 atcagtgcta ttccacaaat cattcagact ggtattcaac ttttgacatc acttgtgaca
    51841 gctttgccgg atatcatcaa tgcgattgtt gcagctattc cagtgattat tcaaaatatc
    51901 atcaatgccg tgattggggc aatccctcag ttgattgacg caggtattca gttacttgtg
    51961 gcattgattg gtgcgttacc gcaaattatc acaacgattg cgaatgccat gccgcaaatt
    52021 atcaacggaa taacaggaac tttggtaaac aacatcgaca aaatcatcaa tgctggtgtg
    52081 aagttgcttg tggcattagt tcaaaactta ccagcaatca tttctgccat tgtcaaagca
    52141 gtaccgcaaa tcatcacagg gcttgcgaat gctttcggtg agtatgtttc aactatggga
    52201 actatcggga agaacttgat tacaggattg tggaatggta tcgctgatgc tggaagttgg
    52261 ttacgcaaca agatttcagg attctttggc ggtgtagtag acaacatcaa ggacttcttt
    52321 ggtattcatt caccatcaac actgtttcgt gatgaaatcg ggaagtacat ggcacaaggg
    52381 attggcgtag gttttgataa ggaaatgact ggtgtttcta aaacgatgca gaaagctatt
    52441 ccaacaaaac tttatatgcc aagctttgat attgataccg atatccacgc tgccgttgat
    52501 agcacattct cgatgcttga tttaggtgct aaggttgatg ccttaacaaa catcacgagt
    52561 gaaatgttgc caacgctact taaagcaatg gacattaaag tcgtcttgaa tgatggaaca
    52621 ttggtcggca aacttgctcc agaaattgat aagagcttgg cgacgttgaa tcgtagacgt
    52681 gtagcatatt agtgttggat tttcacattt tgactgtata ctataactaa cgagtgttta
    52741 ctgaagagat ggtggtaatc atatgttgaa gcataaattt aaagcaaaga aacctgcact
    52801 gataggatat attctactgc ttgctattgg attattttta acggcagtaa gatggtatag
    52861 cggcataaat catggttttg tattcatcaa caatcagatt aattctcaca tttcaaactt
    52921 cgcattaagc tttcttttct atttggtgat aggctatctt tggttgctta tgggaacgaa
    52981 tttaagggtt cttactgcga tgggagctgg tctgattgtt gcaaatattg tatgtgaaac
    53041 gatgatgact tttctgaata caccagatct aatcgatgca atatatggaa tttccggcgt
    53101 gctggttgta tacgcgtatt tagcgattac caagaaaata gggctggaag aaacaaccga
    53161 ataggatgat agattataaa ctttaggatt agacattgtt gtctggtcct tttttagtta
    53221 tttagatggg cggtggtcaa ttgaacgcat ttattttaga cggaacaatt aattcacgga
    53281 ctgatttagg tttgcgaatt acggaagtgc cagttattcc agtgacggaa cgtgtcattg
    53341 atagtattga gattgatgga cgtgaaggag atttgaccat tctgaaaggc tggaaagatg
    53401 tgactttcgg ttttaaggta gtgatttgga aatctgatgt ttggactagc tggcagataa
    53461 ttttgccaca aatcctaaac gctaaaacta tcgcattttc aaatgaacca aacgtgtttt
    53521 acccaatcaa gtatgctaag gcgagcggac taactcagat tttatcttcc atgtgggagt
    53581 ttgaaatgga gttaacctgt tcgccttttc gttataagac caatgtagcg actatcaatc
    53641 gaaccacgtc aggaacggtc aataatcccg gcaacattta ctcattgccg aagattaagg
    53701 tctacggcaa tggaactagg acactcacga taaatggcaa gcctatcgtg ttaaatctga
    53761 cgaatggcaa cctgacacta gattcggatt tgaaagaatg cttttacggg gatgtggcag
    53821 ccaatcaata catgacaggt gactttcctg agtttcgagt cggcagtaat actgtgactc
    53881 ttggtacagg tataaccaaa gtagaaatag aaccgaggtg gcgatactta tgattacgct
    53941 ttacaacaaa acagaatcaa atttcaatca taacggtctc gctgtccttg atggcaacat
    54001 tctgaacccc gtggtgagtg aggaattgaa cggactcttt tcctttgaat tcgattatcc
    54061 aattcatgct ccacacagtg aggaattgtt gccagaaatg attgtgaaag caccagtacc
    54121 agaactgtcc gaccagcttt tcaggattgt tgaacgagat gatgcacttg gtggtttttt
    54181 gcacatagta gctcatcata ttttctatga cctagccaca aactttattg aagatactta
    54241 tatcgtcaac aagaacgggt cgggagcttt gacacaactg cttggtgcaa ctcaattcat
    54301 gcaccgtttt accggtacat caaacatcag tgcggtcaat aatgtccgct tggtgcgact
    54361 aaatcctgta gaggtgttgc ttgatagtga cctagagaat ggttttcaaa gcagttatgg
    54421 tggtgaaatt gtacgcgata acttcacgat ttcaatgtta acgactcgtg gcagtaacaa
    54481 cggcgtacaa atccgagaca agaagaatct gacaggatat aaagctgatg ttgattactc
    54541 tggaattgtt actcgcatta tgccacaagg gtatgatgga ctatttcttc ccgaaaaata
    54601 cgtggacagt ccaaacatca accaatacgt ccagcctaaa atcaaagtca tcaagtacga
    54661 caacgtcaaa gttggtgatg aagaggatga gtttactcaa gccgaggcct atgatgaact
    54721 tcgcagattg gctgggttag aatactcagc taacaaggtc gatttgccaa aagccaccta
    54781 tgatgtggag tttgccccgc ttgagcgaac tgaagaatat aaagattttg cacagcttga
    54841 aacgattaat ttgggtgata ctgtttcagt tattcatgcc gaagatggat tgaacatcac
    54901 agctagaatg gtctcataca aatacgatcc atttctgaaa gcgtatatct cagtaacact
    54961 cggaaacatc acgccaaaat tcacggatgt ggcgaaggac atcaaccggg tggacaacaa
    55021 ggcggagcaa gccaaagacg atgccaatta cgcattgagt tcggcaaatg gtaaaaacac
    55081 caacttctat ggcccgacaa ctccaaataa tccgaaactc ggtgacgttt ggtacaaaga
    55141 aaatggtgac aagtttgaaa tgtgggtgta tgaaatccgt gatggcgtca ctcagtggta
    55201 tccacttatg acagatatga cggcagaaga agtaaagcaa gccgtggaac aagcacaaaa
    55261 ggatgccaat actgccactg aaaaggccaa tcaagcattt aacgatgctg tgaatgcttt
    55321 gaatgcagct gaaagtgtag gtgcaagtgt tgaaacgctt actacatccg ttgaacagaa
    55381 tactagcaag atttcattag tggcgagtgg tatgtcaact cttgaaggac gagtaatcac
    55441 agctgaatca actctgacag tgcaagctgg gcagattgct agtaaggcaa gtcaatcgac
    55501 ggttgataat ctgacagggc gtattacaac tgcagaaagc accatcaccc aaaatgctga
    55561 tagatttgag ctatctcttt ctaaaacaaa taaagcggtc aatgttttag ccggtatgag
    55621 cattgcagat ttgcagtggg cagttggtgg tctgaataca aatgatggaa aagaaaatac
    55681 ctatggtgga tacgttcgct caggttttac aagagttaga aatggcgaaa agtatctact
    55741 tcaaacattt gtgggtggga aactttactc tacttacgca acggcttatc tgctttatta
    55801 caaagaggat aagtcctttt tatcgtacac ccaatttgga aacaccacca caccgcttac
    55861 tgttccaagc agtgccgctt ttattcgcat tcgtttaaca actagtgatg caccagaaac
    55921 catcagttgc tatatgttga aaacggatgt gacaaatggc tatgttgatt tatccgagat
    55981 aagtaacgtc gtaaaactta cagcgaccac tgatgcctta ctgttaagtg tttcagaaac
    56041 caaagaagca atcggcgttc catttaaagt caatcaatgg gaacaaggaa gtttgagtac
    56101 cactgacggt tcagaagtgt ccgcaaccaa ttatctccga agtgagttcc ttgatgtcaa
    56161 agtgggagat aagtatatcg ggcaactgcg tgacggaact caagctgctt tctactatca
    56221 ttactacggc atggatttac cttacattga ttttgtccca acggcaaaac aatttagtga
    56281 aaccctaacg gcgggaactt atactaatgc gaccattgcg agttatctag taagtaagaa
    56341 cgttgaatcg atgagtatta caagtggcgt gacaacaaac ccctattcaa attcaggaga
    56401 ttatctggtc atttacaaat tgccattgga aggcaaagtc gctccgagtg taatttcgtg
    56461 gaatggtcgc aaggatacat cagataatat tgtcttgctt tatacaggtg gtgcttggga
    56521 acagatttcg accgttacaa gtagcagcaa tgccacttat tcttggacgt taacggctga
    56581 gcagatatca gggattacta ctgacagcgt gtatctagct ttcttttcag ttaagactgg
    56641 cacatatgct gggatttata attcaagcaa cacacctttt actctaaacc cgtcggatgg
    56701 ttcaagttat catcaaatct cttatactgg ttcgagcgga gcaattacag tcccgagtgg
    56761 cacattgaaa atgcgagtcc gagtgaacac gagcatcaaa cccgatgaat atgaaggaaa
    56821 tatttatcct acgaccacaa ggcaagacta taccaatgcc aacacattat attcagcaat
    56881 tctcatgcag aaagacttaa tcaatttacg agttggtaaa ggagacgtga ttaatcaaat
    56941 caatatctca cctgaaagta ttttgattgc tggaaacaag gtgcacatca ctggtcaaac
    57001 aaccattgat agtgcagtga ttagttctgc catgatccaa aatgcggcca tcacaaacgc
    57061 aaagattgca gatgccacaa ttcaaagtgc aaaaattgca agcattgatg cggcgaagat
    57121 taccacagga actttgtcgg cagcccgtat tggcgcgaac tcgattacgg ctgataagat
    57181 tgctgctaat atcctaacag cattgactgc gagttcgtcc attcgaatca caggaactac
    57241 gattggatat tattcaggaa gcactcttgt gactgaaata aactcgcagg gaatgaccat
    57301 tcgacgtggt ggaacgacag tcggaacaat tggtgcaaat aacatcagtg gtcactctga
    57361 ttggcgtgga ttggtatttg atcttgagta tggaacagag tatatgtcat gggctcataa
    57421 agattcctcc tctgcgagtg tctacaccca gaagctttct tggtatgcga gaacattgca
    57481 gtctggtatg gagaaggggt tccactttga cgacgacatt tatttaccat caatgcttcg
    57541 tatagggaca caacatcaaa tatctgttgg tgggattaca tttagtagtt ccctttatac
    57601 taagattggc tcaagttccg gcagcgctgg tattgcattc aacggttcta atctgatgct
    57661 cggagatgat gggaagtggg ttgactttgg agtcatccgt gaaatctgtg ccaaacttgc
    57721 gggacgaaca atcgctttac caaccggttt cagtagcaac ggtacagcga gtggttggta
    57781 caatccgcag tcgtttaatt caatgaccac atacagttag gaggccgcca tgaacgaaga
    57841 aaaagaagaa gtgatagtaa ctgaacctat cgaggcagat attccaaagc caatgaaagt
    57901 tgggacggat atggttgatt tgcttgtgat ggtcgtggat gatctttgtg tccagattgg
    57961 tgatttaaag gagaagatag ataatgctca aaattagaaa tgaagatttg gtggacgtga
    58021 tgagtttctt aaaaggtttt gacttaccac caaagattag ccgagtgcga acgaagcttg
    58081 caaaactcat ccaggtgaag gttgatgaac tctatcaaga cgaggtagac ttgcttcaaa
    58141 aatacggaaa gaaagatgag cacggcaatt tggtccaaca tgacggtgcc ttttcactca
    58201 tcccagaaac cgcaactgaa tatcacaaag aaaaggcaga cttgctcaac gaagaaaccc
    58261 acatcaacgt ggatgaactt caagacaagc tgcctttatt ggttgctgga tttgaaaata
    58321 gtgaagtcac catttcaggg aaagatgctg aaatgctcga tttaatttta gaacaattgg
    58381 aggatttgta aatgaaagaa gtgtggaatt ggattcaact cgccatcgca ggtggtggag
    58441 gagtgattgg atggtatgtg ggaggaattg atggtttgat ttatacgctt gtaacttttg
    58501 tggtagtgga ctatgtaact ggtctgcttc gtgcaatcaa cgaaaagaaa ctatccagca
    58561 agattggggc aaagggcatc actaagaaaa tcatgatttt tcttttggtt ggtgtggctc
    58621 atatgcttga tgtggaagtc atcaaaggtg gaaatgtact gcgtgatgcg gtcattttct
    58681 tctatatctc aaacgaagga atttcactgc tggagaatgc cgtggcgatt ggactgccag
    58741 tgccggaaaa gttgcgtgaa atcttgcagc agttacacga aaagaaggag gaaacagaaa
    58801 atgggaatta gtaatctagc aacaaaatat ggatttcagt catttagcca ttactcgaat
    58861 ggtcgtagtg gtgccaaagt agataagata gtgattcatc acatggcaac caccaactat
    58921 gatattgttc ctgatatttg gaaaacgcgt gaggcgtcag cacactacgg tattggaaaa
    58981 aatggagaaa ttcgagcata cgtggacgaa ggaaacacag cttggcacgc tggtaattgg
    59041 aacgccaata tctcaagtat cgggattgag cactgcaata gcactggatc accgaactgg
    59101 ggtatcaatc aagcaactat tgatgcaagt gcaaagttgt gtgcagatat tgccaaacgt
    59161 tatggtctag ggaaattggt ggtgaacaag aatatcttcc ctcactcaca tttttcggcc
    59221 acatcatgtc cgggacagtt gcttggtaaa ctgcaggaga ttgcggataa agccaatgcg
    59281 attaataatg gttcatcaac cccaagcacg ccagctagtg gtctgtatcg tgttcgcaaa
    59341 tcatgggctg acgccaagtc acaaattggt gcctataagg acttgaacaa tgcgaagcgg
    59401 gctgcagata gccattctgg ttataaggtg ttcgaccaga acggtaatca agtttatcca
    59461 tctgggtcat caacatccaa cgtgaaaact cttgtggttc aagtgagtgg tctcaacgtt
    59521 cgcaacaagc caagtttaag tggtgcggtt gttggttcat acaataaagg cgcaacttgg
    59581 acgcttccga agtcagagaa gatgacgatt gcggatggtt gggtatgggc tcgttgcccg
    59641 attggatatt gtgctgtcgg taagaatact ggtaagacgg aatctgacga ttatattttg
    59701 atttcataaa tttaatcgga acaaaattat gtccggattg ttgacttaaa atatcgaacg
    59761 tgttattatt aattcaacca gaacataaat ttgttccgat tgaattatag aaagaggtat
    59821 cgatatggtt acgtttaaag atttgttacc agaaccaaca gaaagccaac aagctttcat
    59881 tttagatcac gggaaaacag aagatgatgg tcagttgaaa tacgctgatg atgctaaaag
    59941 ttacggttgg aacatgcgcc aatatggtaa attgaaagcc ggcgcggttg ttttgaatcg
    60001 tcatccgggg aaaattacta aagaccgtaa gtgggaaatt tacgggggcg gatacgttga
    60061 gagcatttct gaacctgatg aagctggaaa tgttacggca gtaattacac acgcatttac
    60121 aatcgaaccg gcaattaaac aaggtgattc atttattgaa aacttcgatt ggaatacacc
    60181 tagcaaaaag aagcgtaaaa agccgaattc atgggcctac ttctgggatc agtatggaat
    60241 gaatgaaatt acctatgccg attttgttgc attgattgaa aatcgtcatc ttatcccaat
    60301 cgatgataca caatcgttgc cgtctgaact ggatcttaca aacgaggaag ttgaagaaat
    60361 tgagcaagaa agttcaaaag gatttactgt tcttgttgat gaagtgggac caaatcgcac
    60421 gaatgggact cagaaacgaa aattcactgg acgccatact gattgggagc gtgtcaacaa
    60481 ggcaaaacaa aaaacgggtg ctctaggcga agaaatcgta cttgatttcc tcattcagga
    60541 agcagaaaag aacaaaacaa aacttcctga acatgtttct aaaactgaag gagatggtca
    60601 tggctatgat atttgtgcgt ttgaccaatc aggtaatgaa atccatattg aagtgaaagc
    60661 ttcaaaaaca aacttctctg acggatttga aatgagtact aatgaggttg ctgcctccct
    60721 tggagataca ccatataaaa tttactttgt tcacgacctt gatgtggcgt caaaagtttg
    60781 caaaatcaaa atttatgatg gcccaattac tgaagaaaac ttcacgatgg ttccaacgag
    60841 ctacaaaatt ttcaataagt agtttgacgt ctttataatt caaaacagcc tcacaagttt
    60901 tattgtgggg cctttttttc gtctagtttt ctttaaggta ggtgtttaca gcattttgca
    60961 ttaggattaa atgatttctt aactgaaata cctgctcttt gtaattgatg cagaaaagag
    61021 gcgattctat tgattccata cgattattca tcgcgtttat attggcttgt aaaaactggt
    61081 ataaagcttc ttcagcctct atggttttaa agcacttttc aaattcttgt ttgaagcttt
    61141 catcaaattc ctccagttcc tttgttgtcg cagatttgca aaaatgttca tattcttttt
    61201 ctaacccttc catatcaaaa ctactcataa ctttctcctt cccacaattg atatttatat
    61261 tgttatttat tggatgaagc ttgtaaaggc ttatttgatg tttgggaagt aaaaagtcaa
    61321 aatttacaaa gaataattcc tactatatat aaacaaactt agaagcctgc aagagtgatt
    61381 cgtttcattc ttgcaggctt ttttcgtttt gtgggttcga gtcggttatt tctttcgcat
    61441 atggatgaaa ggcaaaggaa aatttgtgat tgggtttact ttcatggatt ttcttcgcct
    61501 gtatcttgag gaggcgaaag caatgactaa caagaaaaca gaaattaact attcagatga
    61561 agcagcctat tacatttcac agcagatcct tctgaactta cttgttaccg gtaaaatcaa
    61621 cctggaagaa tacgtcaaaa tcgacaataa aaaccgggaa acattcaatc cttttttatc
    61681 aaggataatg gtctgaaacg ttgatataac ggcactttag agctaacatg tcacctaatg
    61741 gaaaggagtt gagctgatga aaacgataca taaattggca cgtccagagt tgccggaagc
    61801 ttccaaacta aaggtggcag catacgctcg tgtatcaaca tcaagtaatg aacagctggc
    61861 aagtttacaa acccagatta ctcactatga aaatcacatc aagaaccatg aacaatggga
    61921 ctatgtcggt gtttattatg atgaagggat ttctggaacc aagactgtaa aacgtgatgg
    61981 tttaaaacga ttgattaagg atgcagagct tggaaagatt gatttgattc tgacaaagtc
    62041 tatttccaga ttttcaagaa atacagttga ctgcttgaat ctggttcggc agctaactga
    62101 tattggtgtc accatctttt ttgaaaagga aaacattaat actggtgata tggaaagcga
    62161 gttgttgctt tcaattttat caagcttggc agaaagtgaa tcctattccc attcagaaaa
    62221 cttgaagtgg gcgattcgaa accggatgtc taaagggaaa ttcaaagctt acccgccgta
    62281 tggctacaag cggaaaggct atgattttca catcgttgca gctgaagcaa aagttgttaa
    62341 acaaatgttc cagtgggcat tagatggcat ttcgactgga atgattgcca agcggttaaa
    62401 cgatggaaaa caattcacta aacgtggggc aaattggcaa gcaacatcag tcagaagtat
    62461 tttgaaaaat gaagtttata ccgggacttt gacgatgcaa caaacctttg ttgatgacca
    62521 gtacaaacga cgcataaaca agggcgaatt gccaatgtac cagatcaaaa acaatcacga
    62581 acccatagta tctaaagaag attacgaccg agttcaagaa ctcattcgga gaaaagctga
    62641 ggccaaaggg aatactggtg acgggaaaaa gtacaccaag cgatacgcat tttcaggcaa
    62701 catcatctgt gataaatgcg gcagcaaatt caagcgggtt cacacttatt ggaaaggtaa
    62761 tacgaagaag gttcagtgga agtgtacggg gcatcttaaa aataaagata gctgcgatgc
    62821 attaccaatt acggatgaag atttgaaggt tgcttatttg accatgttga ataagttgat
    62881 tttcggacac aagttggttc tggaaccttt tatgaagtct ttgaataaag caggtgcgaa
    62941 tcaggaaggg ctcgaagtgc ttgctgatga aatcgacaag attcaagaaa agcttgaggt
    63001 tctggcaagt ttgactgctt ccagtatttt atcaacgaag gtgtctttag aagaacaaag
    63061 caaacttcaa caagagaggg ctgtcgcaca agaaaagcag cgcctcatca ttgagtcagt
    63121 caacggcaaa agcactcagc aaattgaact agaagcctta tacaaattcg cgcggatggc
    63181 agagatgttg acggactggg atgaagattt attcaagaag ttcacaaaca gcattacagt
    63241 ttacaaccgc caagaagtcg gatttgaact taagtgcgga ctacttttga aagagaggtt
    63301 accagaatga acaatacaat tccatacggg tatgtcatta agaatggaga aatcaacgtg
    63361 gatgaacagc aagctcaaca aatacgtgag ttcttcaata tttaccttga gggtgttggc
    63421 ttacaagaag ctggcaaacg ctctggcatt aacaagccgc atccctttat gaaacgtatt
    63481 ttgatgaatg aggtttactt aggaaatgag atatatccaa aaatcattga tgaggatatt
    63541 ttcttccaag cggatctgga gcaagaacga agaattaagt tctttaaaaa acgcaaggag
    63601 atcaaagtgc cagatacgaa ggtaataccc actcatttta aatctggcaa gattaccgaa
    63661 aagtattctg acccattcaa acaagcgcaa tatgcatatt caaaaatcaa ggaggttaag
    63721 tgaaatggca ggaaaacagg tgacggttat tccaatgaag cctagacaat gggcagcaga
    63781 gaataccgaa gagaaaccaa agttgaaagt ggcagcctat tgtcgtgttt ctactgaaat
    63841 ggaggaacag gcgagttctt acgaagcaca ggttcaacac tatacggact acattcaaag
    63901 aaatcccgat tgggagcttg taggaatatt tgcggatgaa gggatttcag gaactgggac
    63961 gaaaaagcgt gacggtttta accggatgat tgaggcgtgt caaaacggcg aagttgagta
    64021 catcatcaca aaatcaattt cacgctttgc ccgtaatacg gttgattgct tgcaatacat
    64081 acgacagtta aaagaactga agattgcagt gttttttgaa aaggaaaaca tcaatacaat
    64141 ggatgcgaag ggtgaggtac ttttgaccat tatggcaagt ctggcccagc aagaaagcca
    64201 gtcactaagt caaaacacca agatgggcgt acagtatcgt ttccagcagg gacagctacg
    64261 tatcaaccac aatcattttc ttgggtacac taaggacgaa gatggtaact tggtgattga
    64321 accaaaggaa gcagaagtca tcaaacgcat cttccgcgaa taccttgagg gcagttcctt
    64381 gcaagaaatt gctaatggtc taatggctga tggcatccta acgggaggga agcgaaagct
    64441 ctggcgagga gaaggagtca gattgattct tcgaaacgag aagtacatgg gggatgcgct
    64501 gcttcaaaaa acatatacta ccgatttctt gacaaagaaa cgagtgaaga atgacgggag
    64561 ttacgctcag cagtattatg ttgaagatag ccatccggcc attatcccaa aagatatctt
    64621 catgcaagcc cagcaagaac tagaccggcg taaatcaatg aaaaacaaaa agtcacagtg
    64681 cttttctggg aagtatgcgt tatctggtat cacagtttgc ggtgactgtg gcaatgctta
    64741 ccgcagagtt cattggaaga atcgaggaac tgtgtggcga tgcaaatcca gagtggataa
    64801 acgagaacat aattgcacgg gtcgaacaat ctacgaaaca gacctgcatg gagctgttat
    64861 caaggcaatc aatgaaacgt taatcgatcg tgatgctttt cttcagcaat taaatgacaa
    64921 tatcaactca gttatgactg atggattaac tgaccgattg gcagaactag acagtcagtt
    64981 gaaagagctt gaagctgaaa ttattagcct ggcagttggc ggtcagggtt acgatgaatt
    65041 ggcaagccaa atattttcac taagagatga acgagatgcg gttgcaaaac aaatagcagc
    65101 gaatactaat ttgcaacaac gggttgatga gatggtagtc tttgtaaaag aacacgatgt
    65161 catcaatgag tataatgaag tgcttgttcg aaggttgatt gagaaggtca ccatttttga
    65221 aaagaacatt gttgttgatt tcaaaagtgg ggtgagagtg actgtagaaa tttaatttga
    65281 atagatagga ctagacgtta atgtgggtag ccatattaac gtctttattg attggatttc
    65341 ataaagtgat tgatgatata atatattatg taaaaaatag gctgggggcg aagttctgtg
    65401 agccaatgga ataccattga tgaattaagg gatattttta ttcgtaaata tacggattct
    65461 gagttgagta atgaacttga aaaggcctgc agcgaagaat atgaattgat gcgtgattat
    65521 aatgggcgtc agattttaga gttattacag aatgtagacg atgcctatgg agatataaaa
    65581 tcagaaaatg atgctagcaa tgacgaagtg aaagttcaga taacatataa aaataatatc
    65641 ttggaagttg ggaatacagg cacttcattt tcaaaagata caattgaaag attgtgctta
    65701 ggaagagcga gtaataaatc atcacaaaat atcggaaata aggggactgg ttttcgttcc
    65761 ttgctaaacg atgctgagtg ggtcgaactg tattcgggag aatttgctat tcgatttagt
    65821 gaagaattca caaaaaagct ttttgagcag tatgtatcaa gagattctga caaattcagt
    65881 gaattaattt ttgagcagaa gaaaaattgg aaaaaagaag attatgattt atgttttcca
    65941 ataatgaact gtcctcaacc aataaacaaa attgattctg gttttgatac attaattcga
    66001 gtaaaactaa aagaaactaa tttaagtaaa ggcacgagtg tttctaaaca acttcagcag
    66061 ccattttata agtcactgtt atttttacca aatataacaa aaattgttat tgaaacaact
    66121 gatgatgaaa aaatatcaga gaaaattgtg gatggaaatg atgtattgat agaaaaaagt
    66181 gatgttactg gtcaatcacc gtctgaagaa tattttgttt ttgacaaaga atctatgata
    66241 ggagataaag ttgctaaaat tgcactagcc gtttctaaag attctgatta cgattactcc
    66301 aaagaaaaac tatattgcta ctttccagta cgtaattttg gtacaccaat tcatgcactt
    66361 ataaatgcac cgtttataac aaataattca cgtgatgacg tcccggatga tagtgaacaa
    66421 ataaataaga aaatatttat tgaggtgctc aaatttattt ctgaagtagc agaaaaatta
    66481 tctaagcctg aatatgaaga catagcatta aaaatggtaa ctccttttaa agagaataaa
    66541 ctatgggact tagatgtatt tgacttaatg aacgaattct tgggtatttt atctaatgcc
    66601 caaatactac cgactgtcaa ttggcaatat atttctatca aagataatcc aaaacttttc
    66661 cttcaagaat tccctgagga gctaataggt gagacatttt ccaccttgtt gcaattatta
    66721 gaaacaaacg aaaatgacca actagtacaa gaacttgcta attataatga atacactggc
    66781 ctgtcgtttg ttgtagatga actatctgat aaaatcaaca ttctttcatc ttcttgggaa
    66841 aatagaataa ggacaaaagt gttcttatgg tggagtgagc aatataaaag ttctccgatt
    66901 gtccctaagc tattaaagga caatagtgac aattggttga tgaaatcaga taaggtattc
    66961 ttaccgaccg atacaggtgt tactgtgctt ccaaaagagc tgtcttcttg ggttagttta
    67021 agcattttga aacaaacata tgttgatgag ttaatttatc agattaagac ttcatcaagt
    67081 tggaaaaata aatgggaaga aacagctaat gcatacaaag ctgaacgtac aggagataaa
    67141 cgtattctag atgcattcag tgaaaaatat cttgcaattg aattcactga gcaaagcagt
    67201 tcagatttga tcattggaac gattaatcga caaattgaca ctgttgaaaa atctatcagt
    67261 ttcattaatt ggttttatga taactacaat gaaaaactat cagctggaag tgagctgtct
    67321 aaagttccat ttaatcttcc aaacgttgct ggtgacgttc aaccttccaa taaaatgtat
    67381 attggaaaag agtatggcaa tgaattagga gataagcttt ttcaagatac agattacacg
    67441 gcattgaaaa aaatagattg ctctagtttt gctgaggaga gagattttct gtcatttatt
    67501 cttcgatgtg gtgtttccaa atatcctaag atttatgata acgattcttt aaaaaatgat
    67561 gttggattta aaaagtttat ttataacaaa tataagaatg aaataaaatt tacgaatatt
    67621 aattatttaa cttctaagta catcagtaat tttgaagaat taatagacaa attagatacg
    67681 aaagacattg tcaattggct caatcaagac gaagaattga cagatttatt atcttcggta
    67741 aaaaaagaat caagtgcaag tcagcaatcg aactggagtg gattgtactt taattcaaat
    67801 gagtatgtga aatacatttt gaataaaact gcatggatta agattgatgg agaaaaatat
    67861 agcccgagac aaattgtgct atatgaaaag ctccaggcac acgttccggg attgtatgga
    67921 gtttctgaac aaagcttaat ggaactcttg gggcaacgca ttgttgaaca gttggatttc
    67981 agaaaaaata tggcgatgtt ttcggatgat gaaataagag ctatattagt tgagttacca
    68041 aaattcgata aaggcgaaat aagtcgcaga ttatataatg aacttataag aaataaaaaa
    68101 ggaatgaatc ctacttattc taccgaagga ttatcagtct tagccaaaga tggcttcttt
    68161 tatccaaaca aagaattaag gtatgctgat aagcgactgc ctaaatcaat agaaaaacaa
    68221 atgcgtttta tttacgtgcc tgaaaaagct agtacaacta cgatatatga atggcttggg
    68281 gttgaacgct tcaaaacaaa tttaaaattg gaaacatttg agttattgga agattattct
    68341 gaggatttca aaaaagagat tgatgatatc aaaactgcgg tattatctac gatagatgac
    68401 aatagtaaga atgttagttc attaaaacgt attcagatta ttccatgcag caaaatatct
    68461 gctaaagatg ttgagcaaga tggtcagtca atcgttttgg aagattatta ttttgtggaa
    68521 agtaaaggaa aattctactt aaaattacct gctaattcta ttgaaattaa tcaattacgt
    68581 ttgattgata cgttttcttc aacaatcgta gacatattca agcaggtgtt aaatctaagc
    68641 ttagatttga atttgattga attgttaatt tcccgagata cggaacataa acaaagaaaa
    68701 ataactgaag aattcggtgt agataagtgg aatgaatcgt ttgagctatt gtttagcgaa
    68761 aatgcgttaa ataaaaaagt taatgacttt ttgttatcaa atggactaaa tgaagaggaa
    68821 tatcctacca tttttgaaat tgactattca tatgcattaa aagaagaaga gtatcaattg
    68881 ttggcgatag ctctagaaag catttcaaaa gatattgagg atttgaatgg attgtcagaa
    68941 ttgattcatt tggatattcg tgaattttgg aaactgaaat taagatctta tctagataag
    69001 aaagcggaac attaccgcca gattttatat agcaatgcaa ataaaagcgg tgatgaaata
    69061 ctcaaagaaa cattcctaga aaaaattgat agatacaaag attatcaatt tgagatttct
    69121 gaaattccaa attcagttca agtcgacatt gagaaagaaa gtaagaagag atttccagag
    69181 ctaagtgtgt caattactga tgaaattgac attgatggcc tgtatgataa taatgtcgaa
    69241 gagttagtca cggttgcagc tattacaata gatgattttg attattatat tagaggtcat
    69301 caaaaagttt cttccttact gtattttggt attccgtccc gtttaagaga agatatactg
    69361 atattcgtta ctaaagaaaa tgatgttgat ttgaacggag aaaaatctgg tatctctatt
    69421 gaaggaacat caactgttca aaccattctt gaaccatcga atagtgttag taggaaaggt
    69481 aagaacacac gaaatggaga aagaagtaaa cgagattatg ataatagaaa ctccgcaaat
    69541 gatgatgctg gtgaaacagc agaacaaatt gcttacgctg aattgactca gaatcaaaaa
    69601 cttaatgtaa tttggaatac gaaatattct agtaaatctt cagatcgtaa taagcagcca
    69661 ccaaacggaa ttgtttgtga tatgtgggtt catgacccag agaatgggaa tatgtacttt
    69721 gaggtaaaat catcaattac agaatttgaa atgtcgatta atgaatataa ttctatgtta
    69781 gataaccctg actcttacga ggtcgtttta gtcaatcgtg ataccaaaga gataagtcgt
    69841 cataaatttg atgaacttga tggattgaaa caggcaagta gctatctttt caaatttaag
    69901 caaaagagaa gtgacggttg agtgatttgt ttccacaaac ctattcaatc tatcaactca
    69961 aaaatcataa acaaccatta ttatagtagg aagatacaga gtgtactctc aacgcatatc
    70021 gaattagtcg cttcattcgt taaaggagaa taaaaatgga ccaaaaagaa aaaatgaaag
    70081 aattgatcaa aaagaaacaa ggtggaggcc gatccaaaca aatggaaaca cctaagaatg
    70141 atcgtaaaaa catgagaaaa ggccctaaaa tctttaataa atagacgtgc taaagaaaga
    70201 ctaagaagtg ttaacttttg ttattgtttc ttagtctttt ta
//
LOCUS       DASJSZ010000004        64887 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.004, whole genome shotgun sequence.
ACCESSION   DASJSZ010000004 DASJSZ010000000
VERSION     DASJSZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.004"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1421)
                     /locus_tag="U4B03_000232"
     CDS             complement(<1..1421)
                     /locus_tag="U4B03_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN2067112.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1673..2854
                     /locus_tag="U4B03_000233"
     CDS             1673..2854
                     /locus_tag="U4B03_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2067113.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2914..4542)
                     /gene="treC"
                     /locus_tag="U4B03_000234"
     CDS             complement(2914..4542)
                     /gene="treC"
                     /locus_tag="U4B03_000234"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEN2067114.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4993..7704)
                     /locus_tag="U4B03_000235"
     CDS             complement(4993..7704)
                     /locus_tag="U4B03_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2067115.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7714..8415)
                     /locus_tag="U4B03_000236"
     CDS             complement(7714..8415)
                     /locus_tag="U4B03_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2067116.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8601..9200
                     /locus_tag="U4B03_000237"
     CDS             8601..9200
                     /locus_tag="U4B03_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2067117.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9281..10294
                     /locus_tag="U4B03_000238"
     CDS             9281..10294
                     /locus_tag="U4B03_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2067118.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10402..11319
                     /gene="rbsK"
                     /locus_tag="U4B03_000239"
     CDS             10402..11319
                     /gene="rbsK"
                     /locus_tag="U4B03_000239"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEN2067119.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11347..11742
                     /gene="rbsD"
                     /locus_tag="U4B03_000240"
     CDS             11347..11742
                     /gene="rbsD"
                     /locus_tag="U4B03_000240"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEN2067120.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11742..13115
                     /locus_tag="U4B03_000241"
     CDS             11742..13115
                     /locus_tag="U4B03_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2067121.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13472..15616
                     /locus_tag="U4B03_000242"
     CDS             13472..15616
                     /locus_tag="U4B03_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN2067122.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15668..16708)
                     /locus_tag="U4B03_000243"
     CDS             complement(15668..16708)
                     /locus_tag="U4B03_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2067123.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16985..18787)
                     /locus_tag="U4B03_000244"
     CDS             complement(16985..18787)
                     /locus_tag="U4B03_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2067124.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18801..19553)
                     /locus_tag="U4B03_000245"
     CDS             complement(18801..19553)
                     /locus_tag="U4B03_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2067125.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19562..19924)
                     /locus_tag="U4B03_000246"
     CDS             complement(19562..19924)
                     /locus_tag="U4B03_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2067126.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20100..20972)
                     /locus_tag="U4B03_000247"
     CDS             complement(20100..20972)
                     /locus_tag="U4B03_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN2067127.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21023..21511)
                     /locus_tag="U4B03_000248"
     CDS             complement(21023..21511)
                     /locus_tag="U4B03_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN2067128.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21649..22440)
                     /locus_tag="U4B03_000249"
     CDS             complement(21649..22440)
                     /locus_tag="U4B03_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEN2067129.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22495..23103)
                     /locus_tag="U4B03_000250"
     CDS             complement(22495..23103)
                     /locus_tag="U4B03_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEN2067130.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23096..23413)
                     /locus_tag="U4B03_000251"
     CDS             complement(23096..23413)
                     /locus_tag="U4B03_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2067131.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23569..24297)
                     /locus_tag="U4B03_000252"
     CDS             complement(23569..24297)
                     /locus_tag="U4B03_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEN2067132.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24456..25337
                     /locus_tag="U4B03_000253"
     CDS             24456..25337
                     /locus_tag="U4B03_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2067133.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25574..26617)
                     /locus_tag="U4B03_000254"
     CDS             complement(25574..26617)
                     /locus_tag="U4B03_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2067134.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26620..27288)
                     /locus_tag="U4B03_000255"
     CDS             complement(26620..27288)
                     /locus_tag="U4B03_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2067135.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27430..29766
                     /locus_tag="U4B03_000256"
     CDS             27430..29766
                     /locus_tag="U4B03_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2067136.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29881..30192)
                     /locus_tag="U4B03_000257"
     CDS             complement(29881..30192)
                     /locus_tag="U4B03_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067137.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30373..31848
                     /locus_tag="U4B03_000258"
     CDS             30373..31848
                     /locus_tag="U4B03_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEN2067138.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31895..32479
                     /locus_tag="U4B03_000259"
     CDS             31895..32479
                     /locus_tag="U4B03_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEN2067139.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32481..32864
                     /locus_tag="U4B03_000260"
     CDS             32481..32864
                     /locus_tag="U4B03_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEN2067140.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33181..33465)
                     /locus_tag="U4B03_000261"
     CDS             complement(33181..33465)
                     /locus_tag="U4B03_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEN2067141.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33817..34527)
                     /locus_tag="U4B03_000262"
     CDS             complement(33817..34527)
                     /locus_tag="U4B03_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEN2067142.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34524..35996)
                     /locus_tag="U4B03_000263"
     CDS             complement(34524..35996)
                     /locus_tag="U4B03_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2067143.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36134..36607
                     /locus_tag="U4B03_000264"
     CDS             36134..36607
                     /locus_tag="U4B03_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2067144.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36760..37311)
                     /locus_tag="U4B03_000265"
     CDS             complement(36760..37311)
                     /locus_tag="U4B03_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2067145.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37416..37946
                     /locus_tag="U4B03_000266"
     CDS             37416..37946
                     /locus_tag="U4B03_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEN2067146.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38429..38872)
                     /locus_tag="U4B03_000267"
     CDS             complement(38429..38872)
                     /locus_tag="U4B03_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2067147.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38888..40048)
                     /locus_tag="U4B03_000268"
     CDS             complement(38888..40048)
                     /locus_tag="U4B03_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2067148.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40555..42255
                     /locus_tag="U4B03_000269"
     CDS             40555..42255
                     /locus_tag="U4B03_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067149.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42357..43190)
                     /locus_tag="U4B03_000270"
     CDS             complement(42357..43190)
                     /locus_tag="U4B03_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEN2067150.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43494..45659)
                     /locus_tag="U4B03_000271"
     CDS             complement(43494..45659)
                     /locus_tag="U4B03_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2067151.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45919..48213
                     /locus_tag="U4B03_000272"
     CDS             45919..48213
                     /locus_tag="U4B03_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN2067152.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48206..48871
                     /gene="pgmB"
                     /locus_tag="U4B03_000273"
     CDS             48206..48871
                     /gene="pgmB"
                     /locus_tag="U4B03_000273"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN2067153.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48864..49871
                     /locus_tag="U4B03_000274"
     CDS             48864..49871
                     /locus_tag="U4B03_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2067154.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49893..50912
                     /locus_tag="U4B03_000275"
     CDS             49893..50912
                     /locus_tag="U4B03_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2067155.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="U4B03_000276"
     CDS             complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="U4B03_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN2067156.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52669..54090)
                     /locus_tag="U4B03_000277"
     CDS             complement(52669..54090)
                     /locus_tag="U4B03_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2067157.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="U4B03_000278"
     CDS             complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="U4B03_000278"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN2067158.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56501..57757)
                     /locus_tag="U4B03_000279"
     CDS             complement(56501..57757)
                     /locus_tag="U4B03_000279"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEN2067159.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57869..58074)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58337..59638
                     /locus_tag="U4B03_000280"
     CDS             58337..59638
                     /locus_tag="U4B03_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN2067160.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59707..60306)
                     /locus_tag="U4B03_000281"
     CDS             complement(59707..60306)
                     /locus_tag="U4B03_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2067161.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60560..61441
                     /locus_tag="U4B03_000282"
     CDS             60560..61441
                     /locus_tag="U4B03_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2067162.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61454..63064
                     /locus_tag="U4B03_000283"
     CDS             61454..63064
                     /locus_tag="U4B03_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEN2067163.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63141..63800)
                     /locus_tag="U4B03_000284"
     CDS             complement(63141..63800)
                     /locus_tag="U4B03_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2067164.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64083..64748)
                     /locus_tag="U4B03_000285"
     CDS             complement(64083..64748)
                     /locus_tag="U4B03_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEN2067165.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT        19457 a        13218 c        11704 g        20508 t
ORIGIN      
        1 tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg atcactgaat
       61 tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata ccttacgcca
      121 cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt tgaacgtttc
      181 gttgttccat tcttaaacgt aaatgccatc tctttctcac tgatcttgat tgaagcaata
      241 tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt atcctgattc
      301 aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata cgtatctact
      361 gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc gtcttcaaaa
      421 taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag cgggttttct
      481 gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag atagttttct
      541 ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc taaagataca
      601 cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc aaaataaata
      661 tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc cgcagacggt
      721 tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc taacgctaga
      781 tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt aaaggtttct
      841 tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt ttttgtccat
      901 acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc tacctgcttg
      961 attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg gccttcttcc
     1021 ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag cataccttct
     1081 gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc ggggacccat
     1141 gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt tgccatgtct
     1201 ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa ctctacttct
     1261 ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt cgtcatcacg
     1321 ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt taacatacct
     1381 gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac tcctcttctc
     1441 atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt tacgttttag
     1501 caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct tattttaatt
     1561 gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct atcattttcc
     1621 ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa gagtgaaaaa
     1681 agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct cttactatgt
     1741 tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga tctcgtttat
     1801 attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt ttgcactgat
     1861 cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt cgaggaactt
     1921 gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa gcatatggct
     1981 tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag atttgtttca
     2041 agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg attcatttaa
     2101 tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta ttggaaatta
     2161 ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag ctatttttat
     2221 tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc aaaaatttta
     2281 tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa atttgacaaa
     2341 gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt catttatcct
     2401 tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta gtgttttagc
     2461 tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg ccttctttcc
     2521 aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg tcattgtctg
     2581 gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg gtgatcgttt
     2641 acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc taatgggaat
     2701 tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg ttacttttat
     2761 tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag agtatgaaaa
     2821 ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat cttacgagaa
     2881 tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag caattgcctg
     2941 gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat cgataagaat
     3001 attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat agaaatttgt
     3061 taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt ccggatgatt
     3121 tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc tcagccgaat
     3181 caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt ctacattaat
     3241 ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc ctgcttgttc
     3301 tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt tgatccgacg
     3361 aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat tcaaactttc
     3421 tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga tttcttctcc
     3481 catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca tctctgctcc
     3541 ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct gatcatgatt
     3601 attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc atgtatgaaa
     3661 tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc cattttcata
     3721 atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac taggttctgt
     3781 atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga tgatagtgga
     3841 atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga ttggtttgtc
     3901 cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt ctttaccgat
     3961 aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa aacgaacaac
     4021 tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt gtgtccgatc
     4081 atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac ttccaccaaa
     4141 ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat aatgctggtc
     4201 ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca gcaccatatc
     4261 gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga attcctccat
     4321 cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt tgtctttttg
     4381 agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat tcagatacgg
     4441 gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt ctttgtaaga
     4501 tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt acctccattc
     4561 aactgattta ttacctacca caacttatat atacaagtta tcatttatta ttataactga
     4621 accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg cccaataaaa
     4681 aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa atattcttgt
     4741 ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc tcaaatgtaa
     4801 aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac ggctttttta
     4861 caacctctga ttcatggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa
     4921 aatatgaaaa actattttgc cgtttgtttc tctaatactc aggtcaaacc gctgatttca
     4981 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5041 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5101 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5161 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5221 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5281 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5341 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5401 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5461 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5521 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5581 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5641 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5701 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5761 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5821 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5881 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     5941 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6001 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6061 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6121 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6181 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6241 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6301 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6361 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6421 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6481 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6541 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6601 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6661 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6721 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6781 ttgagataat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6841 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6901 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     6961 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7021 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7081 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7141 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7201 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7261 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7321 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7381 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7441 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7501 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7561 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7621 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7681 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7741 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7801 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7861 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7921 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     7981 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8041 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8101 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8161 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8221 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8281 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8341 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8401 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8461 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8521 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8581 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8641 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8701 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8761 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8821 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8881 aggattcttt gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt
     8941 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9001 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9061 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9121 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9181 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9241 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9301 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9361 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9421 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9481 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9541 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9601 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9661 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9721 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9781 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9841 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9901 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
     9961 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10021 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10081 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10141 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10201 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10261 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10321 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10381 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10441 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10501 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10561 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10621 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10681 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10741 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10801 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10861 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10921 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    10981 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11041 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11101 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11161 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11221 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11281 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11341 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11401 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11461 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11521 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11581 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11641 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11701 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11761 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11821 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11881 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    11941 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12001 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12061 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12121 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12181 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12241 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12301 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12361 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12421 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12481 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12541 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12601 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12661 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12721 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12781 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12841 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12901 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    12961 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13021 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13081 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13141 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13201 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13261 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13321 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13381 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13441 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13501 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13561 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13621 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13681 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13741 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13801 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13861 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13921 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    13981 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14041 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14101 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14161 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14221 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14281 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14341 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14401 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14461 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14521 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14581 ccaacctggc gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14641 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14701 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14761 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14821 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14881 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    14941 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15001 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15061 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15121 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15181 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15241 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15301 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15361 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15421 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15481 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15541 agctgcaact gttcctgtac taaccttgtt aaggttagac aaatcattct ggcttgatct
    15601 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15661 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15721 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15781 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15841 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15901 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    15961 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16021 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16081 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16141 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16201 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16261 gcgtataccg tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag
    16321 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16381 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16441 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16501 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16561 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16621 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16681 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16741 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16801 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16861 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16921 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    16981 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17041 agatgaaata caatacgaag aatatcgtaa acggaaccca aatatctttg tatggatttt
    17101 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17161 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17221 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17281 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17341 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17401 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17461 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17521 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17581 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17641 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17701 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17761 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17821 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17881 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    17941 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18001 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18061 tgaaataagt tcctaatacg atcgtaacta aggctacccc tataccaaag acttgatagg
    18121 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18181 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18241 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18301 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18361 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18421 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18481 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18541 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18601 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18661 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18721 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18781 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18841 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18901 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    18961 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19021 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19081 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19141 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19201 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19261 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19321 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19381 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19441 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19501 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19561 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19621 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19681 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19741 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19801 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19861 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19921 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    19981 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20041 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20101 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20161 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20221 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20281 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20341 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20401 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20461 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20521 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20581 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20641 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20701 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20761 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20821 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20881 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    20941 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21001 ataaatatct aatgttactt ttttaatttt tttttcgaat acgcgccatt acaccttcta
    21061 gttctaactc tttgtcttca gtgatgtacc aaccgcttgc ctgtgtccct ccggctgcct
    21121 gcggtaaata atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta
    21181 ccaagacctc tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga
    21241 taaatcgttc cttcttccca tcatacttca atattcctgt atcgataatc ggtaaagaat
    21301 aataatccag ctctttcttc accgtcaata aatgtttgaa aacttcatgg tacacttgct
    21361 ctaattggcg accttctgaa ttgttcgggt aattaaccat cactttttct ttgtctggat
    21421 catagaaggt ttggcttagt tgagtgatat aagtatgtaa tctttccatt tctgttttta
    21481 acttttctct ttgaccttct tctaccttca tatactcttt caccctgttt tctttttaga
    21541 tattttcttt cttattttac tatagttatg tgcgttaagc gatgaaaaac agcaaagatt
    21601 ggaaggatct ttgctgtttt ttcttaaaat atccagttga gtacaggatc aatctgtttc
    21661 attgtcctct tgatccgtcg taatattgat cgatgtgtct ttagcagtga tctttagctt
    21721 tttctgtcct tgttctaaca aagtatttgt tattggctgg tagtcttttt caatataaga
    21781 ttcaccagac gtttggatag tatagccact gttttccatc tcatgcaaag ataaattgcc
    21841 accgtttagg atgacttttc cttctttgct gatcaaactg tcttctagat ggaagttgct
    21901 gttttcacct gtgacgatca agttatccac attggcctca tctaatcgaa gtgagcctaa
    21961 ttcatctttc gcagttattg ttcctcctgt aaattcattg aacgataccc agccatcatg
    22021 tgtcttagta ttcacttttt tgattgatac ttcgttgata tcaattgcac tattattccc
    22081 ttcgattgtc aattcagata attcatgatc tgcagggaca acaatagtga tttgtgcact
    22141 tgtgtcttct aaactaatga ttgcacctgt tgattttttg actgcattca cagtcaacac
    22201 gccatttttc tcctttattt ctggcttttc tgtcttatca taagtcgttt ttatctcaaa
    22261 atccaatccg cgtatgaccg aaactgtttg attttctgca tggatgacga ttttgtttgc
    22321 atccttgatt ttttttgtct cagtaaccat atcgatcaac cgaggaccat tatcccaagt
    22381 cagactcttt tgagcgccta atgtataagt tgcacctgct aggaggccac cagtaagcat
    22441 cagaccaata ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac
    22501 gcccgctgaa tgcagatctc tttttgtaat aaaacgtcgt cccatccatt taacaaagtt
    22561 catcatgaca taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa taaatactgc
    22621 gcttcctgtg gcgaacagac cgacaccgat aaagaataat ccactcagaa tcgattgtcc
    22681 aaacacactg attcctgaga tgatcatgaa taggccaaca cctaagatac tgatacataa
    22741 aacatagata ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa taaccaatat
    22801 aaaccccagt gctagtggaa tgaggacagg agaagcaaat aaaccgataa tgattaacca
    22861 aatcaggcga atttgtcttt ttggattttt cactaactcg ttttctgctt gttcttcttc
    22921 tgaaaagtaa ctgattttta attttaaggc aaactgcttt ggtgtaccat attcatcaat
    22981 gatctgatct aatgtttttc cagaatctaa aaaatattcc tcataatact ggatcaaatc
    23041 aaaacgatcg tcttcaggaa tttcttgaag atgatccaca acttcattca aatattcatt
    23101 catttgtgct ctcctccaat aaccggtcta aagaatctcg aaaaacaatc cattcatcaa
    23161 taatttcttc taaatgacga cttcccattg gtgtcaacga gtaatatcga cggtttctgc
    23221 cttcaaaagg ttcatcatag gtctcaagat atccttcttt tttcaatctt ctcaaaaccg
    23281 gatatagtgt agattcactg acagagagac tttcttgtac gatttttgtt aaggcatacc
    23341 catacatatc ttccttgctt aatgctgcta gcacagtacc atcaagaaga acagttggaa
    23401 tctggatgtg cattacgtca ccttctattc tcaagtgtaa tattttattt caatctatat
    23461 tatacaacat ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa
    23521 gatttgatcc tttttagtca gcaaaaaatg gcttctcttt gatttttatt ataacagtgg
    23581 cataatccaa tttgtccaca tccagctcag tggcatgata acgatcattg caggtatcat
    23641 gtcagctact gggaacattt tcacgttgat cattctaaaa ccggtagcga ccatcaagaa
    23701 tccgccgcaa gctttaaaat ctaagatcat gtctggtgta gtcaatggat aaataaattt
    23761 tgccaataag aacaaaatat aaaaaataat gaattgggga attgctatca cagaaacgac
    23821 ataccctaaa ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat
    23881 caaaatcgta ctgtctcctg tcatcccaga atctaaactt ccatagatcc ccgtaccact
    23941 tgcacaaaat agaacaatga ccgtcactaa tgtgctgata aattcatcgt gggtcatacc
    24001 aagcttttca ctagggaaga ttttagaaat aggtacttgc atcagcgcag cccccttatt
    24061 tatcccatta cctaaatgaa caatcaaacc aatcgctgtt cctatcacta aagcaaaaat
    24121 aacagccggc atatttttca tcggcgcaat ggaatagatt cccatcccca tcgaacacac
    24181 accaaaaacc atcgtcagct gtgctttaaa atgttcactt aatttatttc ctactaatcc
    24241 gccaattagt ccgccaaaca aaacggaacc tacattgatc aaaactccag ttggcatcaa
    24301 aaaactcctc tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt aattattgct
    24361 ttcgctgcaa gaaagattgt aacagacaga gaagattaca ccaattcatt ttttgtgtta
    24421 aagtattctt tattggaata agtaggagga atatgatgga tatccataaa ttcgaagttt
    24481 ttcttgattt agccgaaaca atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc
    24541 aaggaaatat ttctaaacag attctttcgt tagaaacaga attagacgtc aaattattcg
    24601 aacgcgccca tagaaaaatc gaattgaccg aagctggaag cttattgctc ccatatgtaa
    24661 aaaatgtcgt agaacaatac catgctatgc aagaagtgct tgaaacctat acgaaagaca
    24721 acaatctttc attaaatatc ttaacgatac cgacaatgat caattataaa ggtttttcca
    24781 aaatcaccga gtttttgaaa cgccatcctg aatttacagt ccagttgaaa gaagtggaaa
    24841 gtgtcgaatt aacttacaat gacgccaatg aacatagtga cacgatccac tttgctagaa
    24901 gtttccaggc cccaccaagt tcgattgagt ggttgcctac tgaaacagac gattttgtag
    24961 cagttctacc taaaaatcat cctttagcta gtaaaaagaa attggatcta agcgaactga
    25021 aaaaggagaa ctttttgctc cttggcccca ctacgaactt gtatcaacct gtactagatc
    25081 tgtgtcgcga agctggattt gaaccaaaaa tctcttataa aggagcaaga atcgacttga
    25141 tcatcaatat gattgcaaat gacatgggga ttgctattgt tatggaaaaa acagtcaaga
    25201 atctattaaa agaaaatact gtgattctcc ctatttctcc aacaacagag agttatcttg
    25261 catttactcg taaagtcggt gagcattctt tggcttcaga tacattttgg gagtatttaa
    25321 atcaagaaga aaactaagcc ctacattttc tgtccatcta tccacccaaa aaaagagagc
    25381 gacgcatgac tttcgctctc tttcttgcat catgtccttg tttcaatgac acctctaaca
    25441 tttctcgtag ttcaataaga aagcagcgat ttttatgcca aaagaaccgt aagaacattt
    25501 ccctaatcac aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt
    25561 tttattcttt ttcctattgt ttacttaacc gtatcattgc tgtcgttcct actccttcga
    25621 tactgctata atcaatacgt ccatcatgca gttctagaat ttttttaacc agtgaaagtc
    25681 ctagcccatt cccagaaatt tgacgacttg tatctccttg ataaaatttt tcaaacattc
    25741 gatctctggt ctcttcattc attcctacac ctgaatcggt cacttccagt acaatctctg
    25801 tggctgtctc aaatagtttg atatgaatct gtccattgat ttgattgtac tttatggcgt
    25861 tatccatgat attcagccac acttggtaca aaaactcttc attgcctgtg taatttactg
    25921 cagcaagttc gatatccaat tctagctgtt ctttctccca tttaggctga aaaaaaacga
    25981 ttacttctct gatttgttca tctaatcgat actctttctt ttccagttgg atacgctggt
    26041 tttccagttt gttcaatttt aagatgttct ctgttaattg tgaaagctgc gtgatacttt
    26101 cgataatccg atataaaaat atttggcgtt cttcgtcaga gagatttggg gcttgtaaca
    26161 attgaacata tccttggatt gttgctagtg gtgttttgaa ttcatgggat acactggaca
    26221 caaagtcatt cctcagtgtc tcgatactat tgagttcttg gaccataacg ttgaaatctt
    26281 tatacagttg ctgtacttct gctactttct gctgttcatc cattcgaatc gaaaaatctc
    26341 ccgttgctac cagactcata tttgtcccta gcgcactgat tggatgcagg atacgccgcc
    26401 caacaaaaat cgatattcct gtacccacga ccatactgaa ccccgcaaag acgaataacg
    26461 ggtataacgt tggttttcca gccgattcag caggcgagat agaatgtcga gacaataaga
    26521 aaataaaacc gagaaaacac agcagtgtca ctaagatcga aatgaagaca atactagcaa
    26581 aatagatcca taattgtgaa aatagctttt ttttcatctt taaataaccg ccttatagcc
    26641 tagtccccgt actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg tccttaaacg
    26701 tttgatatgt acatcgatcg ttcgttcttc tgtattcgta tctaatcccc aaatatcatc
    26761 catcaactgt tggcgagtga agatcttgtt gggataagac agtaatttat acagaagcag
    26821 aaattctttt tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat aagcatctag
    26881 ctctaatttt gtttgtccga ttatcaagct actgctgtaa ttgatctttg aacgtcgaag
    26941 caatgcttcc acacgtagga tcatctcatt gacatcgatg ggttttgtca tataatcatc
    27001 tgctcctaat ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg taatcattag
    27061 aacaggtgtg tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc catccatttt
    27121 cggcatcatg atatctgtaa tgattaattc tatacgtgtc gcttctagtt ttttcagcgc
    27181 atcctctcca ttcaatgcgg tgatgactga aaagcccgct cgttccaaca cagaacggta
    27241 aagcaactgt aattgcggat catcttctac tactaaaata cgattcattg atctccctct
    27301 gttttttgaa aaatttctag attttttctt gtagttcatc ttcagttaac atttatttcg
    27361 tatactacga tcattgttct gatatactta ttagtatacc gaatatttca atacgggaga
    27421 agggtgatta tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg cgaaaccacc
    27481 accaaagcat tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt agcgatttta
    27541 ggtcctagtg gttctggtaa aacgactatg ctgaatgtga ttggtggact agataattat
    27601 gattccggtg atatggtaat caacggaaag tctactaaag actttaagga cagtgactgg
    27661 gatgcctacc gaaacaactc tatcggtttc atttttcaaa gttacaactt gattggtcat
    27721 ctaggcatta ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc taaagacgaa
    27781 aaacgaaaac gggcagaaga tgcattgcac cgagtcggct tgacaaatca tatgcataaa
    27841 aagccaaatc agttatctgg tggacaaatg cagcgagtgg ccatcgctcg agcactagcc
    27901 aatgacccag atatcctttt gtgcgatgaa ccaaccggag cgttagatac agaaacaagt
    27961 atccagatca tggaattgat ccaagagtta tcgaaagaaa aattagtgat catggtcaca
    28021 cataatcctg aactggccca tcaatacgca gatcgaatca ttgaattttc tgatggtaag
    28081 atcttaacag attcccatcc tcacatcgaa cgaccaaaag atgatcaatt taacttacgc
    28141 agaacaaaga tgagtttttg gacagcgtta aagctttcct tcaacaatat ccgaacaaaa
    28201 aaaggacgta ctttcttgac ctcctttgct tccagtattg ggatcatcgg aatcgcgatc
    28261 gttctgtccc tttcttctgg attccaaaaa caaatcgata acacgcaagc tgaaacgatg
    28321 gcgaaatttc cgattactat ttctaaggta acgactaacc agactcgtga cgatgccgga
    28381 ttgggtgcaa gcaaagcgga ttatccagac agcaaaacga ttactgcaaa agtcagcgaa
    28441 gaagataaag cacagcacac gaataagatt gatcaaacat acgtcgatta cgtaacagac
    28501 atcgatccta acttaagtaa taatatcgga tttacccgta ctacaggaat caatctttta
    28561 cgtgatgtaa atggcaaagt acagcctgtc agcttctcga atcaaaatcc tgatacggaa
    28621 tctctttctt tatctagtac gatgtcagca atgacaggtg tgggagtgtc aagtttccct
    28681 acacagcttg acactagcaa agaaaacttt ttaaaagaca attattcttt acttgccgga
    28741 agttatccag cctcagcaac agatgtcgtg ctgatcgtag acggcaataa taatactaat
    28801 atcaacgcat tgaagaatct tggatttgat gtgaaagaag acgaaaaact tgattttgat
    28861 gatatcgttg gtacaacatt caaattagta aacaacaata cttactatac aaaacttcca
    28921 actgggaatt ttatccctaa tacagattat gatgcgatgt atcagaatgc ttctgatgaa
    28981 ttgaagatct cggggatcct acgcgtgaaa tcttcttcta caatgaatct cttatctcca
    29041 ggaatcgctt atagcgatca attaacgact caaatcgtca acgaaaacaa agaatcagaa
    29101 atcgtcaaag ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa agttgacgaa
    29161 aatgccaaac aaacattgct tagttattta ggtggagaca gcttgccttc aagtatcatg
    29221 atttatccga acaattttga agacaaagaa aagatcttgg attatctaga tgattacaat
    29281 aaaggcaaat cagatgaaga taaaatcatt tatactgatt tagctggaac gatgactgaa
    29341 ctgactggcg gattgatgga tgctatcact tatgtcctta ttgcttttgc agggatctct
    29401 ttagtaacaa gtatgatcat gatcagtatc attacttata catctgtgat tgaacggaca
    29461 aaagaaatcg gtgtactaaa agctttaggt gcacgtaaaa aagacatcac tcgcgtattt
    29521 gacgcagaaa cgtgtatttt aggtatttct tctgggatat taggtgtgtt tattgcatgg
    29581 ctagctacgt tccctatcaa tagcatctta tacaacatga cggatctaaa aaatgttgct
    29641 caactaaatc ctgtccatgc gattattcta gtgatcgtct ctactatatt gacgatgctt
    29701 ggcggtcatc ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc tttgcgtgca
    29761 gaataaggtt gtgacaacca catttagatt tgagtcataa gagcgctgaa atagtagaaa
    29821 acagcaagaa acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt taattatttt
    29881 ttataattct ggtatacttt tctttccttt gtaccacaac tcttctggcg tatacctgtc
    29941 ttttccattt atgaatactg ttccatctgc ttcaatcgtt acttcagtcc caatccacat
    30001 attacgagaa agtgcgagaa gattttttct ttttcctctt cctttttttc caaattgcaa
    30061 ggcatcggtt atctcttctg tccaatgaga tttcacgtat tttacttctc cgttttcaaa
    30121 tgtaactttc aaaatattcg ctatattttc cacttttact gctttaggaa atgactcctc
    30181 catttttttc actttccttc ctccaacact aagtatgcgc tttcttttag tgataagtat
    30241 atcatagttt attctattat caaaaaaacg catccattcc attgtagaat actttaccga
    30301 acaagttgaa ttgttttcct ctttttcacc ttttactata gataggtaaa gacaactaag
    30361 gaggaatctc tcatgacaga tcaaccgtat gatttaagaa aagtcatcga agaactaaaa
    30421 acacttccag gtcaatatca cgaaacagat attgaaatcg atcctgaagc agaattatct
    30481 ggtgtttacc gttatatcgg tgctggcggt acagtaaaac gtccgactca agaaggacca
    30541 gctatgactt tcaacaatat caaaggtttc cccggcacac gtgtgaacat cggaacaatg
    30601 gctagtcgta aacgtgtagg tgaaattttg caccatgatt atcgaacact tggtcgtttc
    30661 ttgaaagacg ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc agacgctcca
    30721 gcacaagaag tcgttcatct agctaccgat gcagacttcg atattcgcaa cattttgcct
    30781 gcacctacaa atactcctga agatgctggt ccatacatca caatgggtgt agtcttagga
    30841 tcagatccag aaaaaacaat gagcgatgta acgatccatc gtatggttct agaagataaa
    30901 gatacgatcg gtatgtacat catgcctggc ggacgccaca ttggtcactt ccaaaaacaa
    30961 ttcgaagcct tgaataaacc aatgcctatc actatcaata ttggtttaga tccagcaatt
    31021 gccattggca cgactttcga accacctacc actccacttg gctacaatga actatggtgt
    31081 gcaggagctt tgcgtaacga acctattcaa atcgttgatg gtatagcagt cgatgaagtg
    31141 ggtattgctc gtgctgaatt catcattgaa gcagaaatca tgccaaatga aacaatgcaa
    31201 gaagatatca acacgaactc aggaaaagca atgccagaat ttcctggcta tactggggat
    31261 gctaatccag ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa agacaatcca
    31321 atcatgcaaa caacgattgg tcccagtgaa gaacacgtat ctatggccgg tattcctaca
    31381 gaagcaagta ttcttgcctt ggtcgataaa gctattccag gaaaagtagt aaatgtctac
    31441 aatcctcctg caggtggtgg taaattgatg accatcatgc aaatccgtaa agaaaacgaa
    31501 gcggatgaag gtattcagcg tcaagcagct attcttgctt tatcagcctt caaagaattg
    31561 aagacagtca ttttagtcga tgaagatgtt gatattttcg acatgaacga tgtagtttgg
    31621 actttcaaca ctcgtttcca aggagataaa gatatcatcg tgatcccagg aatgaggaac
    31681 catccattgg acccatcaga attaccagaa tataatcctg gctctattcg tttccgtggg
    31741 ataagtgcaa aaactatctt tgacggtaca gttcctttcg atatgaaaga acattttgta
    31801 cgtgcaaaat tccaagaagt agaagattgg gaaaaatatt tgaaataatg aatgatctgc
    31861 aactgttttg acatcataaa aaggagtgcg gaaaatggaa caattggcac aacggaagaa
    31921 aaaaatcgtg attggtgtaa gtggcgcttc aggcaccatt tatgccatca acctagtgaa
    31981 gaaactaaaa gaatatccga tgatagaaac acacggaatc atcagtccat gggcaaaaca
    32041 gaacttaaag ctggaatccg acttatcact aactgaattc gaaagcctgt ttgattacca
    32101 ttattcaaac aaagatttag gtgctgctgt cgccagcgga tcatttttga ccgatggaat
    32161 gatcatcgta ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt taggcgacaa
    32221 cctgattgca cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag tcattgttcc
    32281 tcgtgaaacg ccattaaatg ccattcactt ggaaaacttg actaaattag cacgactagg
    32341 tgtgcaaatc ataccaccaa ttcctgcttt ttataatcag cctcaaacga tccaagatct
    32401 tgttaatcac catacaatga aacttttgga tcaatttgaa attgtaaatg agcattcaaa
    32461 acgttggcag ggtgattaat atggaaaaag aaacgatagt tgaagtaacc aaactaggtt
    32521 tcacgatcca agctgctgtt aaagtaatcg gtaaagactt attgatcact ttaacaggcg
    32581 gagatacacc acatatcgga acagtcacta cattttctcg tgatacagag atgcaaactg
    32641 tacgtttccc tagccacgat ggtcggttcc ataaagacga tgtcttagcc gaaaaaatag
    32701 ctgtgatcat caaaactgcc gttccgggaa gttgtacgat cacttccggt atccatgttg
    32761 accatatctc caatgcacaa atcaacgcct cttttccgat gtcagaagag ttaggttaca
    32821 agattttaga ttggttggaa aaaatagatt tccatatcaa ataaccagtt tattataaag
    32881 atggtgaaaa accattatga aagaagaggt tgtgaaatca gcgttatgac ctgagtatga
    32941 gagaaaaaaa tggaaaaata gcttctcata ttttgccgtt tttgagctaa taccgaggga
    33001 cagctgattg aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt tcgagtagta
    33061 agaaaaattt tgtaaacatg ggtttccaca tttttgcata aatttgactt actaccgaga
    33121 aactggcttt tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc acaacctctt
    33181 ttatttttgc ttcactgtaa ctgctacaat atcaccgatt tcttttccga tgtttttacg
    33241 aatatctttt cgtagtccaa ggatgtagca gactgaacca tctggatttt tcacgcccat
    33301 attcacgata ctgccttcat acggcacatt gtcaaaagtt gcttggactt taactcttcc
    33361 agcaccgaat tcttttctta tatcatacgg aaaaataata tatgccccgc ccttgccgac
    33421 ttcggaagca tagatttttg attcaaaaga ataaatttcc ctcattttat ttctcctatt
    33481 ttttacatct agtatagcat agcaaaaaaa agaaggaact gtgacaaaag tcttgtcgca
    33541 gttccttttt tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag
    33601 tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca
    33661 ggctcacaac ctcttcctta aacggtgttc aaaagcttgc atctacggca ctaagcccaa
    33721 tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc
    33781 agctttttca caacctccta catggtgttc aaaatattat tcgccttttg cttctttcac
    33841 taattttgcg aatgcttgtt gttggaattc agcagctaag gctttcgtac gttgttcttg
    33901 ctctgctttg ctcatttttg gattttcagt cattgctttt accataccgt cttgaacata
    33961 agcttttgct tcactttcca tcgtactttg ctgtttcttc actaattcag ctaacttggc
    34021 tgcttggtcc gtacttaatt ctacccatgt ctctgggatt ttgaatgtat tcactcggct
    34081 gtcatattcg tgattgttat cagcaatatt gaaccagaat ttatacatgt cgtttttgta
    34141 ttcccaataa gtagttttcg tcactttttc agcatcgccg tctgtctttt ctactttatt
    34201 tgtttcgtta tccgtaccag ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag
    34261 atacactttt tcttttccat tccctaaagc tttatacaaa agcatatctg ctccttggga
    34321 atcagcactg gaaacaattt tttctgtttt tgtttccgtt actttttcca ttccaaagtg
    34381 attggacaaa ttagcgacga tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa
    34441 agataaaaag gtttgccatg tttttttagc gaacacgttc gttaaagcaa aagcaagcaa
    34501 gctgacaatc aaaactccaa taatcatgca ttatcccccg ctttcattag aacaggttct
    34561 tttttcttca ataaaaaggc aataataaag ccaagaatcc caaagatgat cgctacgata
    34621 aacgcggcat ggtaacctga cagtgtggca ttgattgcct gatctttata agctaatggc
    34681 gtattatcca atacagattt gcttgggata tttgcttttg tcacattcgt caacacactg
    34741 atcaaaatag ctgtaccaat cgagcttgcg atttgacgga atgtattatt taccgctgtc
    34801 ccatgactga tcaagttatt tggcaatgca ttcataccag aagtcgttac cggcatcatg
    34861 accatggaga tcccaaacat acggatcgca taaaggatca cgatatataa aactggagtc
    34921 gtttttgtca agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg
    34981 gctaaacgtt tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat catagccccc
    35041 atgatcaatg cacccggcaa caacatcaag ccagaatgga atgctgattc tccacgaata
    35101 ttttgaatat acaatggtac gaccatttct gcccctacca tcgccatatt tgttacacca
    35161 gctaaaacag ctgcaatcgt gaatgtagat gatttgaata cacgaagttc caagaatgga
    35221 tgttccaaat gtaattgacg ccatgagaac aaggcgatga aaatcacacc aacaactaag
    35281 aaaccaatta ctttcaagct tccccagccg tcatctccta cactagaaaa tccgtaaagt
    35341 aaactaccga atccaatcgt tgataaaata gcggataaaa tatcaatgct aggatttgac
    35401 aatggaataa cacttttcat taggaagaat gctaaaacca agacaagaac aacgattggc
    35461 agtaccatac caaacaagtc acgccaatgg tagttatcaa tgatataacc agataatgta
    35521 gggcctaaag caggagcaag tccaataacg atccctgaca tccccattgc tgatcctctt
    35581 ttttctggcg ggaaaataga tagcatgata ttttgcaata atggcatcga tactcccaca
    35641 ccgcaagctt ggatcaaacg accgattagt aatgtactaa aattaggtgc tgtaaagcaa
    35701 gtaatcgttc cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct tgaactgaat
    35761 ttattgatca gccatgctgt tattgggatc atgatcccgt tgactagcaa gaaaccagtc
    35821 gttaaccatt gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct
    35881 gtcgtcaata aggtctgatt caaaacggta caaaatgtac caattaataa gacaacaact
    35941 aaaagtgtcc gattatatgg ttttccgtaa atatctacgg gttggttgtt actcatattt
    36001 ctccttcttt ctgaattttt tctgaaacta ctagaatact ttagtcctaa attcgctatt
    36061 tgtcaaccta cttgacacat aagttaatac ggatataatt aagggaagca agaggacaaa
    36121 aggagtgaca aaaatggttg gggcttccct taaacgtttg ttggaaaatg atcaattggt
    36181 tgttggcatc agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg
    36241 ggaacaaaag ggattcattc gttcgatcga agctgatgcg aattgtccta gaaaatatcg
    36301 tttacctaca gtcataaaag tagaactgat caaaaaatat ttagatgacg gatttacttt
    36361 gtcaaaagct gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt
    36421 tttttcaaaa gcaatcaaag atatcgaatt gatcgacgaa cgctatacga ttttacaaat
    36481 cggtaacttc aaagaagaga atgctaagat gtacatcatt catgacaacg agacagacga
    36541 attaacttac aagctccttc ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga
    36601 actctaaacg ccactaatct cctaagtacc aagcttttta ataagtagat gctacataaa
    36661 tacttgaaaa aaggcctgtt tgaacataag aatatccgaa cggtgatctg cgtttctttc
    36721 atatagaaag gaattctcca tcactgttcg gatatttttt tatttttttc ggttactttc
    36781 ttctaataca tctgttagcc aatttgcata ttctgtttcc cgattgatcg catgcgctaa
    36841 aatcaggtag tgtccgtaat tttcatgttt ttctttttca gtaggaaaga tcgttttttt
    36901 tcgctctaat agatgaagtc gtttttcttc atgaaggtca cgctgctgct gaataatttc
    36961 tactaatgcc ggatcattca cgtctttgac gaaatacaat ttcagaacaa attcgtctcg
    37021 attaaccggt agttcattgg tgggtgtatg aatccactct gccaattgtt ccttcccttc
    37081 ttctgttaat tcataaactt tcttttccaa tttttcacct gtgatctcta cttgatgctg
    37141 aatgattcct tgctcttcca tttttgcaag ttcaggataa atctgactat gttttgcttg
    37201 ccaaaattct cctatttcat ttttgaacga ttgattcaat tcatatcctg tcatcttctt
    37261 cttacttaat aaccctaaca aaatatatcg aagtgtattt tttcttgcca tcagttttcc
    37321 ctccttgaca aatctttaaa tccattctat cataaatgag taatcgaaaa catgtagttt
    37381 attacatgtt ttaatttata aggagatgct taaacatgaa agaatttaaa gaattggaag
    37441 attttattgg aactcacttt atctatacgt atgacaatgg ttgggaatat gaatggtatg
    37501 ccaaaaatga ccatactgtt gattaccgta tccatggcgg aatggtcaaa ggccgttggg
    37561 taaaagacca agaagctgat atcgttaaat tgacggacgg cgtattcaaa atcacttgga
    37621 cagagccaac agggacagac gtggcattgg actttatgcc aaatgaaaag aaacttcacg
    37681 gaatgatctt tttccctaaa tgggttgaag aacatccaga aatcactgtt actttccaaa
    37741 acgaacatat cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat ccaaaattac
    37801 ttgttcctga atttgctacg atcacttata tgggtgatgc aggtatagac aatgaagatg
    37861 ttattagcga agaaccatac gatggaatgc cagatgatat ccgtagcgga aaatacttcg
    37921 atgaaaatta taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct
    37981 gacctgagta ttagagaaag aaacggaaaa atagttcctc atgttttgcc gttttttgag
    38041 ctaataccga gggtcagctg attgaacacc gtttatcaag aggttgtgaa atcagcgtta
    38101 tgctttgagt aataagaacg ctagttagaa aatagctttt catattttct aggctagtgg
    38161 gcttattacc gagaagcagc cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt
    38221 tttgacctga gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt
    38281 gagctaatac cgagggtcag ctgattgaac accgtttatc aaagattgta acaactgcat
    38341 attcacacag ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc
    38401 tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc catttcatca
    38461 caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg
    38521 aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc agtgttttat
    38581 tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg tattcccttt
    38641 tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata atttcttgat
    38701 ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat tctacttgat
    38761 tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc agccaaatcg
    38821 atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc attttttctc
    38881 tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct tttacaagtt
    38941 ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca aaagaaacag
    39001 caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat tgatccagct
    39061 tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta gtaagtgttt
    39121 ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac gaatcactaa
    39181 taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt agtcgtccga
    39241 taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac cattgccgca
    39301 tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta taaggaatcc
    39361 gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg
    39421 taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc agtaccactt
    39481 gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata aacgtgtctc
    39541 cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag
    39601 agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc aagacgcctg
    39661 ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt aattggatct
    39721 taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt gtactttcat
    39781 ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca gttaatcgtt
    39841 ggtttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc cgtttttgta
    39901 cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa aatggatata
    39961 aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac aaaagtcgac
    40021 gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt
    40081 tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa
    40141 agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat attttttgtt
    40201 gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    40261 aaactgcgtt atgctttgag taataagagc gctagtcaga aaatagcttc tcatattttc
    40321 taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc gagaggtcat
    40381 gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa
    40441 caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc ttgtaaaatt
    40501 cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg atatatgatg
    40561 aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc atttttagca
    40621 atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa actaattttg
    40681 ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat
    40741 ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag gatcgtgaat
    40801 gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct tgcaggtagc
    40861 tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg attttctatg
    40921 ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt agccgtttta
    40981 ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac tagctggggg
    41041 acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca taaaaagttc
    41101 cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt actgtcttcg
    41161 atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa ccagactgaa
    41221 aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt tctgacagca
    41281 aatatttggg caggattagc tattgtttac agtgggaata agattcttcc tccctttgtt
    41341 aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct ccattaccct
    41401 aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt tcaacaaaag
    41461 aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt tctttctact
    41521 agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag tttgatccag
    41581 tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct tgccctttca
    41641 ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg cattcttatt
    41701 ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga aaatgaaaca
    41761 tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa aacattcttc
    41821 agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca
    41881 atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat actagaaaac
    41941 gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg gaaaaacact
    42001 ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct acagcaaaat
    42061 ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt gaagcagcaa
    42121 aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta tttcattctt
    42181 tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca tagatacaag
    42241 cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct ccaaacggta
    42301 taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta tattttttat
    42361 tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg actatcgatc
    42421 ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa aataaaatct
    42481 atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac agtagctgct
    42541 tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact ttctcttaca
    42601 gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa aagtggtacg
    42661 cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc acttttttta
    42721 tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc tatctttgtt
    42781 ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgaaattg ggagacggta
    42841 aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc atatttatca
    42901 taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccgtgttc ctttaaaagc
    42961 tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt taccggacag
    43021 aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt gatttcttgt
    43081 aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg caattttttc
    43141 tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat tggttttctc
    43201 cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac ctctccagca
    43261 aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag ctattttttg
    43321 tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc actaaacagt
    43381 attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga ggagctattt
    43441 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tccttacatt
    43501 ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat cactacttct
    43561 tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc gttttggatc
    43621 acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc ttctttgaca
    43681 gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg gatcaaaact
    43741 tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat acttgtcact
    43801 tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa gccgtcaccc
    43861 atcatttttt gtgagaatac cggatctttt acttcagata aaggaatcac ttctccagaa
    43921 gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc ttgtccattt
    43981 cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag atcttcgata
    44041 tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt gtctttgacg
    44101 atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt ttctcgatca
    44161 tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt ttcttttcct
    44221 cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc gctgttagat
    44281 acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt agcaaagttg
    44341 aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc gatcacacac
    44401 aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg tgtccgagtc
    44461 agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc catcgcaaat
    44521 gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa cataaacatg
    44581 aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga aaaatacatt
    44641 gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa agccattccc
    44701 atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg cgtccagtta
    44761 tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac taaatcagtg
    44821 gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg tgcgcctgat
    44881 aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag catatgatgc
    44941 aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc tagaatcggt
    45001 gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc ctgtacgttt
    45061 ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac aacaaacgga
    45121 acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata attatagtac
    45181 ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac tcccatattc
    45241 aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac
    45301 agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc aaagatagcc
    45361 ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc gcctgctctt
    45421 tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt accaataatt
    45481 gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca atcaggattg
    45541 atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat aactgctacc
    45601 acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat ctttttcatt
    45661 ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc gttttctttt
    45721 ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc tataatacgt
    45781 gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt tctatgaaat
    45841 attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg tataaaacag
    45901 ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa
    45961 atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt
    46021 ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat
    46081 caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa
    46141 aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat
    46201 atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg
    46261 gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat
    46321 aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc
    46381 tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat
    46441 ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt
    46501 ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt
    46561 gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct
    46621 gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt
    46681 gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg
    46741 cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca
    46801 aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa
    46861 ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac
    46921 tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc
    46981 ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa
    47041 cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac
    47101 aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc
    47161 gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc
    47221 tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga
    47281 ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga
    47341 gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat
    47401 aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat
    47461 ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa
    47521 gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat
    47581 gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct
    47641 ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat
    47701 gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac
    47761 tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg
    47821 attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct
    47881 cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat cacttccatg
    47941 actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag
    48001 ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat
    48061 cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa
    48121 ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa
    48181 ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt
    48241 cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg
    48301 cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact
    48361 ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga
    48421 attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga
    48481 tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc
    48541 tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta
    48601 ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt
    48661 cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc
    48721 gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc
    48781 tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta
    48841 cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta ccagttccag
    48901 agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt
    48961 acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt
    49021 tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg
    49081 tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa
    49141 actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag
    49201 tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt
    49261 ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag
    49321 agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct
    49381 acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt
    49441 tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag
    49501 aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc
    49561 cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac
    49621 aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg
    49681 tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt
    49741 caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat
    49801 ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct
    49861 ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga
    49921 aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt
    49981 ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca
    50041 tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc
    50101 ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg
    50161 aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc
    50221 tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt
    50281 attcagatag tcaagatact gtcattgatt atttatacca aaaccacatc cctttcactt
    50341 tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc
    50401 ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg
    50461 tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa
    50521 tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc
    50581 tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata
    50641 tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt ccagagacac
    50701 tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa
    50761 gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga
    50821 tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag
    50881 agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca
    50941 tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc
    51001 tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    51061 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa
    51121 aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
    51181 tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt
    51241 aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt
    51301 catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc
    51361 acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga
    51421 acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt
    51481 gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc
    51541 ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc
    51601 caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag
    51661 cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc
    51721 tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga
    51781 tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac
    51841 tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc
    51901 attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa
    51961 cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt
    52021 aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc
    52081 taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg
    52141 accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt
    52201 cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag
    52261 tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa
    52321 gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat
    52381 cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat
    52441 gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag
    52501 gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat
    52561 cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg
    52621 acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt atttttcttc
    52681 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52741 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52801 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52861 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52921 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    52981 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53041 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53101 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53161 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53221 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53281 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53341 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53401 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53461 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53521 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53581 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53641 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53701 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53761 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53821 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53881 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    53941 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54001 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54061 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54121 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54181 gggattcttt gagtttattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54241 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54301 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54361 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54421 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54481 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54541 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54601 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54661 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54721 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54781 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54841 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54901 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    54961 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55021 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55081 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55141 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55201 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55261 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55321 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55381 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55441 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55501 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55561 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55621 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55681 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55741 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55801 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55861 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55921 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    55981 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56041 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56101 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56161 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56221 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56281 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56341 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56401 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56461 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56521 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56581 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56641 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56701 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56761 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56821 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56881 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    56941 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57001 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57061 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57121 cttctgcttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57181 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57241 cgatagattg caggatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57301 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57361 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57421 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57481 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57541 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57601 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57661 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57721 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57781 tttatgaaag caggaacctg ctagcatatg taattaatta tttcttcagg ataggatagg
    57841 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57901 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    57961 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58021 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58081 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58141 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58201 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58261 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58321 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58381 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58441 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58501 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58561 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58621 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58681 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58741 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58801 tgattttgtt ttagttaatt tgaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58861 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58921 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    58981 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59041 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59101 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59161 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59221 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59281 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59341 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59401 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59461 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59521 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59581 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59641 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59701 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59761 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59821 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59881 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    59941 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60001 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60061 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60121 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60181 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60241 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60301 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60361 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60421 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60481 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60541 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60601 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60661 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60721 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60781 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60841 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60901 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    60961 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61021 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61081 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61141 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca ctcgatgaag aaggaacact
    61201 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61261 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61321 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61381 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61441 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61501 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61561 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61621 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61681 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61741 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61801 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61861 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61921 agcggattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    61981 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62041 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62101 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62161 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62221 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62281 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62341 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62401 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62461 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtag tcttgatttg
    62521 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62581 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62641 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62701 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62761 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62821 atggcttcct cgaatcggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62881 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    62941 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63001 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63061 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63121 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63181 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63241 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63301 tgatccttcc ctgtcgcttt agctaagtca agcagataga gagcgactcg ttcttctact
    63361 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63421 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63481 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63541 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63601 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63661 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63721 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63781 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63841 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63901 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    63961 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64021 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64081 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64141 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64201 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64261 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64321 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64381 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64441 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64501 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64561 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64621 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64681 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64741 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64801 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64861 aactgcgatg agcatcaccg cagtttt
//
LOCUS       DASJSZ010000005        58632 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.005, whole genome shotgun sequence.
ACCESSION   DASJSZ010000005 DASJSZ010000000
VERSION     DASJSZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.005"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(57..797)
                     /locus_tag="U4B03_000286"
     CDS             complement(57..797)
                     /locus_tag="U4B03_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067166.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(927..1874)
                     /locus_tag="U4B03_000287"
     CDS             complement(927..1874)
                     /locus_tag="U4B03_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2067167.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2003..2767)
                     /locus_tag="U4B03_000288"
     CDS             complement(2003..2767)
                     /locus_tag="U4B03_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2067168.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            complement(2771..3160)
                     /locus_tag="U4B03_000289"
     CDS             complement(2771..3160)
                     /locus_tag="U4B03_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067169.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3295..3837
                     /locus_tag="U4B03_000290"
     CDS             3295..3837
                     /locus_tag="U4B03_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEN2067170.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            3985..4617
                     /locus_tag="U4B03_000291"
     CDS             3985..4617
                     /locus_tag="U4B03_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067171.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4751..4876)
                     /locus_tag="U4B03_000292"
     CDS             complement(4751..4876)
                     /locus_tag="U4B03_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2067172.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5010..5903)
                     /locus_tag="U4B03_000293"
     CDS             complement(5010..5903)
                     /locus_tag="U4B03_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEN2067173.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6085..7011)
                     /locus_tag="U4B03_000294"
     CDS             complement(6085..7011)
                     /locus_tag="U4B03_000294"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEN2067174.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7096..7857)
                     /gene="pflA"
                     /locus_tag="U4B03_000295"
     CDS             complement(7096..7857)
                     /gene="pflA"
                     /locus_tag="U4B03_000295"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEN2067175.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8101..10332)
                     /gene="pflB"
                     /locus_tag="U4B03_000296"
     CDS             complement(8101..10332)
                     /gene="pflB"
                     /locus_tag="U4B03_000296"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEN2067176.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10609..13059)
                     /gene="parC"
                     /locus_tag="U4B03_000297"
     CDS             complement(10609..13059)
                     /gene="parC"
                     /locus_tag="U4B03_000297"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEN2067177.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13071..15116)
                     /gene="parE"
                     /locus_tag="U4B03_000298"
     CDS             complement(13071..15116)
                     /gene="parE"
                     /locus_tag="U4B03_000298"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEN2067178.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15282..15716)
                     /locus_tag="U4B03_000299"
     CDS             complement(15282..15716)
                     /locus_tag="U4B03_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEN2067179.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15843..16481
                     /gene="plsY"
                     /locus_tag="U4B03_000300"
     CDS             15843..16481
                     /gene="plsY"
                     /locus_tag="U4B03_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEN2067180.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16613..17488)
                     /locus_tag="U4B03_000301"
     CDS             complement(16613..17488)
                     /locus_tag="U4B03_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN2067181.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17570..18361)
                     /gene="codY"
                     /locus_tag="U4B03_000302"
     CDS             complement(17570..18361)
                     /gene="codY"
                     /locus_tag="U4B03_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEN2067182.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18379..19779)
                     /gene="hslU"
                     /locus_tag="U4B03_000303"
     CDS             complement(18379..19779)
                     /gene="hslU"
                     /locus_tag="U4B03_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEN2067183.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19793..20341)
                     /gene="hslV"
                     /locus_tag="U4B03_000304"
     CDS             complement(19793..20341)
                     /gene="hslV"
                     /locus_tag="U4B03_000304"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEN2067184.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20378..21283)
                     /gene="xerC"
                     /locus_tag="U4B03_000305"
     CDS             complement(20378..21283)
                     /gene="xerC"
                     /locus_tag="U4B03_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEN2067185.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21481..22857)
                     /gene="trmFO"
                     /locus_tag="U4B03_000306"
     CDS             complement(21481..22857)
                     /gene="trmFO"
                     /locus_tag="U4B03_000306"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEN2067186.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22826..24904)
                     /gene="topA"
                     /locus_tag="U4B03_000307"
     CDS             complement(22826..24904)
                     /gene="topA"
                     /locus_tag="U4B03_000307"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEN2067187.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25026..25883)
                     /gene="dprA"
                     /locus_tag="U4B03_000308"
     CDS             complement(25026..25883)
                     /gene="dprA"
                     /locus_tag="U4B03_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEN2067188.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(25939..26706)
                     /locus_tag="U4B03_000309"
     CDS             complement(25939..26706)
                     /locus_tag="U4B03_000309"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEN2067189.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26699..27559)
                     /gene="ylqF"
                     /locus_tag="U4B03_000310"
     CDS             complement(26699..27559)
                     /gene="ylqF"
                     /locus_tag="U4B03_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEN2067190.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27819..28217
                     /locus_tag="U4B03_000311"
     CDS             27819..28217
                     /locus_tag="U4B03_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2067191.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28496..28996
                     /locus_tag="U4B03_000312"
     CDS             28496..28996
                     /locus_tag="U4B03_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN2067192.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(29079..29630)
                     /gene="lepB"
                     /locus_tag="U4B03_000313"
     CDS             complement(29079..29630)
                     /gene="lepB"
                     /locus_tag="U4B03_000313"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2067193.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29782..30009)
                     /locus_tag="U4B03_000314"
     CDS             complement(29782..30009)
                     /locus_tag="U4B03_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEN2067194.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30006..30524)
                     /gene="msrA"
                     /locus_tag="U4B03_000315"
     CDS             complement(30006..30524)
                     /gene="msrA"
                     /locus_tag="U4B03_000315"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2067195.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30544..31257)
                     /locus_tag="U4B03_000316"
     CDS             complement(30544..31257)
                     /locus_tag="U4B03_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEN2067196.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31260..32156)
                     /locus_tag="U4B03_000317"
     CDS             complement(31260..32156)
                     /locus_tag="U4B03_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2067197.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32314..33156)
                     /locus_tag="U4B03_000318"
     CDS             complement(32314..33156)
                     /locus_tag="U4B03_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2067198.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33288..33941
                     /locus_tag="U4B03_000319"
     CDS             33288..33941
                     /locus_tag="U4B03_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEN2067199.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34004..34531)
                     /locus_tag="U4B03_000320"
     CDS             complement(34004..34531)
                     /locus_tag="U4B03_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2067200.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34551..35498)
                     /locus_tag="U4B03_000321"
     CDS             complement(34551..35498)
                     /locus_tag="U4B03_000321"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN2067201.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35510..37396)
                     /locus_tag="U4B03_000322"
     CDS             complement(35510..37396)
                     /locus_tag="U4B03_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2067202.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(37556..39634)
                     /locus_tag="U4B03_000323"
     CDS             complement(37556..39634)
                     /locus_tag="U4B03_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEN2067203.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39647..39922)
                     /locus_tag="U4B03_000324"
     CDS             complement(39647..39922)
                     /locus_tag="U4B03_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067204.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40284..40739)
                     /locus_tag="U4B03_000325"
     CDS             complement(40284..40739)
                     /locus_tag="U4B03_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067205.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(40893..42101)
                     /locus_tag="U4B03_000326"
     CDS             complement(40893..42101)
                     /locus_tag="U4B03_000326"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEN2067206.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42213..42545)
                     /locus_tag="U4B03_000327"
     CDS             complement(42213..42545)
                     /locus_tag="U4B03_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN2067207.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42693..43568
                     /locus_tag="U4B03_000328"
     CDS             42693..43568
                     /locus_tag="U4B03_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2067208.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43650..44231)
                     /locus_tag="U4B03_000329"
     CDS             complement(43650..44231)
                     /locus_tag="U4B03_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEN2067209.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44237..45496)
                     /locus_tag="U4B03_000330"
     CDS             complement(44237..45496)
                     /locus_tag="U4B03_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN2067210.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45498..46544)
                     /locus_tag="U4B03_000331"
     CDS             complement(45498..46544)
                     /locus_tag="U4B03_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067211.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46779..47054)
                     /locus_tag="U4B03_000332"
     CDS             complement(46779..47054)
                     /locus_tag="U4B03_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEN2067212.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47297..48607)
                     /gene="der"
                     /locus_tag="U4B03_000333"
     CDS             complement(47297..48607)
                     /gene="der"
                     /locus_tag="U4B03_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEN2067213.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(48789..50018)
                     /gene="rpsA"
                     /locus_tag="U4B03_000334"
     CDS             complement(48789..50018)
                     /gene="rpsA"
                     /locus_tag="U4B03_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEN2067214.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(50153..50833)
                     /locus_tag="U4B03_000335"
     CDS             complement(50153..50833)
                     /locus_tag="U4B03_000335"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEN2067215.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(50882..51517)
                     /locus_tag="U4B03_000336"
     CDS             complement(50882..51517)
                     /locus_tag="U4B03_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2067216.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(51583..52986)
                     /locus_tag="U4B03_000337"
     CDS             complement(51583..52986)
                     /locus_tag="U4B03_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEN2067217.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(52973..54004)
                     /locus_tag="U4B03_000338"
     CDS             complement(52973..54004)
                     /locus_tag="U4B03_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2067218.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            54035..54256
                     /locus_tag="U4B03_000339"
     CDS             54035..54256
                     /locus_tag="U4B03_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEN2067219.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(54421..55158)
                     /locus_tag="U4B03_000340"
     CDS             complement(54421..55158)
                     /locus_tag="U4B03_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEN2067220.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(55311..56651)
                     /locus_tag="U4B03_000341"
     CDS             complement(55311..56651)
                     /locus_tag="U4B03_000341"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEN2067221.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(56888..58411)
                     /locus_tag="U4B03_000342"
     CDS             complement(56888..58411)
                     /locus_tag="U4B03_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEN2067222.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT        16051 a        12357 c         9987 g        20237 t
ORIGIN      
        1 ggaaacgccg cagcgtttcc tttccaactg tagccgaata taaatttgta tatcacttat
       61 ttaaaatagc tattttctcc caaagagttt ccttgatcat cgatcaatcc ttgtaatgag
      121 cgttctaaga taccatggct ttttttcgat tttttttctt ctgactgctt tgtttcctct
      181 aacgtttttg ttcgattgaa tgttcgtaca gacggctcac catttctttt ttcttgatac
      241 ggcgtcggtt ctgtatcgaa caacagataa gagtcatccg tttttttcat cgattcgatt
      301 aaatgatcct cagaaatttt gctttctcta gggtctggga taatcgatgc gggtacatat
      361 ttaggaacaa aataagaacg ctcccgcacc ttcgtgtcaa cagatgcgtt tcgtgtagta
      421 tgcttggact ctgtccttgt ttcactttgt ctgacagaac gagaggaatc accaaaaagt
      481 ttttttcgtc ctgtcgatgt gatttctgat tggtaatggc cggtataatc cggtaatttt
      541 cctttatgct tttccaaatt ttccttacgc ggagctgact tcaatccatt tgttgcacga
      601 cgcttgcgac ggattttctc ggtgttcgtg ttcgtgaaat gaaattcttt catctgttca
      661 aacggggact gattctgttc agtcaaaaga tcttcttgtc cgtcaaataa tactcgcttc
      721 ttgtcgttgg ttaattttac gcctaactca tcatcgtacg caggaaaacg gaaatgtttt
      781 cgtctgattt ctttcattca aaccatcctt ctcaaaaatg gaacttttct caataatagc
      841 acagattcat tgttttcagt agcaatgcgc tgattatttt aagaaaatag cgatgaattt
      901 cataaatata tatatagtaa taagacttaa ttcgttgtct ctctgataac tagttcagga
      961 aaaaaacgac gataaccttg actcgcccct ttttcttcta ttttttttaa caacatttct
     1021 ccacatactt cacccatacg tataatatct tgcttgatcg ttgtcaattt aggtgaactg
     1081 atttggtcta aaaatacccc atcaaatcca atcacaccaa attcttcggg caagtgtcct
     1141 ccagcattta taatccctct tactgctcct agtgctagcc gatctgaact acatacaaaa
     1201 caagttttat ctgtaaaatc tgcccagttt tctgtcatat atctttctac ttctgtcgaa
     1261 tgatttggaa accgcaagat tttactagga agatcaactt ccttcatcac agataaataa
     1321 ccagtttccc ttgaaatttc aaaaggctcg tccacttcga taccgatata aataatcgtt
     1381 tgataatctc tttctaaggc atattgcgtt gcttttgctg taccatattc attatcgctg
     1441 tctacataat catatccttc agaattttcc ccaaacaaga caactggttt ttccaaacgg
     1501 tcgatccaat cgaagtcttt ttttctgaca cctgtgatga tgtatccatc acaagaaccg
     1561 acgtcgaagg catcatttgt aactaattgg agagaataat gcgctttacc gatccgttta
     1621 gcgattccca tcagcaaatt catgtaatag ggctcagttg tgtcgatttc ttccagtata
     1681 aataatttga tgatctgcga ccgattcgtg acaagtgctt ttgcagcaat atttggacgg
     1741 taatccagtt cagccattgc tgagaaaact aactctttca attcgtcagt cacctgttcc
     1801 ggatgattga tgactcgaga gaccgtcatt tttgaaacat tggcacgttt tgcaacatct
     1861 gctaaagtgg gcatgctctc ttcctttcta acatgttgtc attcttacta tcaattatac
     1921 caaattttcc taacagagtt tattatagcc caaaataaaa aacggaaaat cctctatcgt
     1981 catgagtgat tttccgtttc aattatttat acttttttat caaatttttt gctaaaaaag
     2041 tggaagcttc ttccattagt tgtatttgta ccatatgtcg ctgtgttttt gaggaacgaa
     2101 aaacaatttc ttttccgtaa gacttatgcc gatttttttc tacaaatcgt tccgtttgta
     2161 tataagggat tttttcgtca gcttgtccat gccaaaagaa cagaggacgt ccagctaact
     2221 tttccggctg caatgacaaa tcatagctct taacccatga aatcaaatca aaatagtctt
     2281 ttggtacgac ataattatat tccgatgcgc gccttacgat ttcttttcca tattccaaag
     2341 gtgcagggga acccatcaaa cacacgcccg ctactatatg aggatgttta gccaacaagg
     2401 cgcatgttgt cattcccccc atcgaaaccc cgccaactgc aagctgatca tgtagaagac
     2461 ctcttttttc taaatgcttg ataagtgtgt caaattcaaa cagatttccg tagatactat
     2521 cccaaaatgt ataggatgga atagaagaaa ccggctgttt gcgctcccca tgattcatcg
     2581 catctggcag gatcacacga atgccctgct gcgcaatttt tcttccttgc gtcaaaacta
     2641 actccttact tgtctgccat ccgtgataat agatgacaac gggaatacgt tcctctctta
     2701 attctttcag aacaacttct aacactggaa tatgatggac agaacgtttc aatataattg
     2761 gattcaattg ttactcacct attttcatta aatttcctga tcgccatagc aaccatgctc
     2821 caacagttgt ctggaacagg ttcagtgtac caaataatat aaatgcagga ctgaatggct
     2881 cgggaaataa gcacagagaa caaagtccca ccactcctaa tgctagcgaa aggagtggat
     2941 aaatagaaaa attcaaaaaa ctggcaacgc taccgaaaat attaccaata aacagtatca
     3001 aaaagaaaaa aacgcttaaa attaaaccat ctgtcagcga catctgaaaa acaatgttct
     3061 ctacctcaat tggctgttga atcgaataga cacctaagta tatactgaga aggagactta
     3121 gcacgtggct ggcacaaaac acacgattga ccgttttcat tttaatcacc taacctatct
     3181 ttataattct attataatca ttttatcatt tttttgtatg tataaagttt caatttttct
     3241 tcacttacga ttttttttga tattgttgtt atataaatga aaggagtttt acaaatgatt
     3301 gatccaaaaa tgttggggat gatcttcctc attaattttg cttatatcac tctgaacacg
     3361 ttacgtttca tgttgacaat gaaaggctat cgactgattg ctccacttgt cagtatggtc
     3421 gagatcacga tttatatcgt cggcttatcc cttgttttag atcgtttaga taatccactg
     3481 aatttatttg tctatgcgtt aggttacgca gtaggtatca gtgtcggaat tttgattgaa
     3541 gacaaattgg cattaggata catcatggtc acaacgattt tgccagcaaa tagcgcagaa
     3601 gataaaagtt tgccgggtat actgagagaa cacggttatg gtgtgacaca gacttctgca
     3661 atggggctgg aaggtgagcg gctagttctc gaaatacttt ctcctagaaa aagcgaacgt
     3721 gaactgtatc gcctgatcaa agaaatcgaa gagcgtgctt ttatcatttc ttatgagcca
     3781 aaatatattt caggtggttt ctggactaaa aaagtgagaa aacgacaagg aaaataactc
     3841 acaaacttaa aaactgtcta atatctaaat taaattttaa atgttagaca gtttttattt
     3901 attattttga ttaaaaaaca tcgacatata ctcatcaatt tgttattata ctttatatac
     3961 ttatcaattt aatttggaga tattatggaa attcaacgta gaaccactaa ttttttacat
     4021 ttaggctctt tttttgcaac tttgacgatc ttattttttc tatgttattc ataccttaag
     4081 acctcacatg cgccttctag tgcattaggc tggttgcttt tggatcattc tgatatcaca
     4141 gaagccgaag aaaatccgtt ttttttgatt ttgtcttctc tttgcggctt actgttttct
     4201 gtttatttcg gtgcctattt ttttcatcga catcctggta cgttaaacga agcttcaagc
     4261 gtattgattg catttaaaac gttttctttt ttagcaattc ttttttattc gtttaatcac
     4321 aatacgcttt tcttctttat catgaatggt ttagtcgtga ttttatccat ttttacttat
     4381 tttatttttt cactcatttt tagccaattg aaatgtccgc tcttctttcg attagttcct
     4441 gtcacctgtt ttatttacag cttgttttcc ttttttgtcc ttgctttaat gccactgacc
     4501 aacccttcga cgatccaagt cgcaggaaat ctgttggata tgctcttcat cgtcagtcta
     4561 ggtgtattta tgtggcaaag aaaaaacaaa caaaataaaa agcattttga agcctgacta
     4621 ttacgacttc ataaatgctt ttttagtttt aaacacttca atctaaaaga ataacccaag
     4681 attggataaa ttattaatga ctcgccattc gtttcttagt gattctggat tatcaaatgg
     4741 acttcctaat ctaatcagct agttcttccc ataaaatgcc atacttgact gaatcataat
     4801 attgattatt taaaaatcta acttttctta tcactacttc ctttgtcatt ccacatttct
     4861 caccattttt ttgcattcct ttgtttccag accatgtagt gaatctaata ggtggtagat
     4921 aggcaaaata ctcaaataat tcttttaacc ataaaggaag agaaaggctc ctcctgcaag
     4981 agtcagtata atttttgctt actgctctct taggttttac tcactaaaac tgttccatat
     5041 tctttgatat aagaaaatgc atgcggtata aatttcttgc tcagctcaga aaagatttgt
     5101 cgtttaagaa aggccatttc ctccattgtc aatgaaggat ccaatgttgg taattcaatc
     5161 tcaaaattgt ttttttcgac aataggtaat tctaatgtga atgattcttc ttcctttata
     5221 gtattcgtca caagtagaga ctgatggaaa atactaggcc ggcgattttt atatttattc
     5281 ttacgtatcc tgtcaacgat aacttctatt tgatcaaaaa agtacgattc atttacgtct
     5341 ccaataagtt cggctaattc tttgaatgcc ttcgttacag gaagctgatt cttctgtaca
     5401 aaaaagtaat tagccaaagt aagcgggaag tgttctatag aggaaagtgt gataaaacgg
     5461 tacttctcat ttcctgcagt tgtcctcagc aattctgtct ctcggctgtt tcttactcca
     5521 taaaaatacg cttctttccg ttcatcttcg aaacaatacg cccaaataac ttctcccatc
     5581 gctaattttt gttcatcaac agagagtctc ttcaattgtt cagctatggt ttcagcagcc
     5641 gaagtggctt gttgcaagta atctttagga tcaaatattg gaaagttcaa atgatatctt
     5701 cgttcttccc tcttgattaa tcctgcatcg atcaataaat caagaaaatg ggaaaattta
     5761 ttttgcgaaa attctttttt caattctctt agaatcgttt cttgttctcg atgttcatat
     5821 agataatcgg tcaatgaaga aaaaagcgga tcatcggtca gtttttgata aaagttttcc
     5881 ttattttttg aataatagtt catatatgtt ctcttttcta tccgttttat tttacttaaa
     5941 aggcttttac gtaaaaagga ggaagaacaa gagtcataat tgactgttat tctcctccat
     6001 ttcttcggcc tttagtatgt tctatgtttc tttttatatc aatcgcttat ccattcattt
     6061 tgaatgcttc atactaccat ttgattagct aaatacagcc gtcaattgag gtacgacttg
     6121 ttttttacgt gaaacgacac ctttaagcaa agcacggttg ttggtcaaag ttgtttggaa
     6181 agcttcttct actttttctt ttcctttacc agcaacaagt aattctgaat cactgtctaa
     6241 gatgtttgtg acaactaata cgaacagatc gtagccattt gctttcattt catcttccat
     6301 tgctgcttct agctctgctt gtcgatccat cacttcatta aggtccactg tattgacttg
     6361 agcgatacga acgttggaac tgcccattgg gaaacttttt gcatccaaat caagaagaac
     6421 cgcagctgat ttatcactta gatttgttcc tgctttcaac atttcaagtc cataagtgtt
     6481 tagatcgact tcagcgattg ctgcaagttc ttttgcagct gcttcatctt caggcgtaca
     6541 tgttggtgat ttgaacaaca atgtatctga aatgatagct gagagcatga ttcctgcgat
     6601 ttctttagga attgttactt ggttttcttt atatagtttt aaaataatcg tacttgtaca
     6661 tccaactggt tctgcacgat agtacaatgg attagctgtt tcaaagtttg caatacgatg
     6721 gtggtctact acagctaaaa tatttacttc gctgatatct gaaatacttt gttggaattc
     6781 attatgatcc acaagcatga catctgcatt ttctgcttta gctgactcta ctactcgtgg
     6841 tgcagatagc tgaaagtggt ctaatgcgta ttgtgtttct tcattaggtg tacctagagc
     6901 aactgcttct gtttctttac ctaattcatt ttgcaagtgt gaaaacgcaa tggctgcccc
     6961 gattgcatct gtatctggat tttggtgacc aaaaactaaa atttttgaca tgtcattcgc
     7021 tcctttgtga tttctcctat ttaataatag caaaaaaacg gaaagaagca atacttatct
     7081 tctttccgat gcgacctatc gcgttaaata ttttgtatac tcttctgtat gaagcaatcg
     7141 tttagcattt tcgacacgtt cttttgtagg tggttcgatc ccttctaatg gatatttcaa
     7201 accaagtgtt tcccacttgt attttcccat cgtatgataa ggaagaatct ctactctgtc
     7261 tacatttgac aaggtttgaa taaagttgtc tagtcggatc aaatattcat cataatcact
     7321 tcgctcagga actagtacat ggcgaatcca aaccggtttg ttgatttttg acaaatattg
     7381 agccatttct aaaatatttt cattgtccca atgcgttagt tttttatgct cttcattatc
     7441 gatgtgtttg atgtcaaata acagtaaatc tgtatacttc atcaattctt gaaaacgtga
     7501 aaagaaaggt tcttcatacg taaaaggttt tccgcatgta tccaatgtag tatgcatctt
     7561 ccgctctttt gcttttttaa atagttcgat caaaaaatcg atctgcaata gaggttctcc
     7621 accactaaca gtaataccac ctttatctcc ccagtaaggt ctgaagcgtt ctgctttatc
     7681 taaaagttca tctgctgtgt attctttccc tccgccaata ttccaagtat ctggattgtg
     7741 acaaaattgg caacgcatgc ggcatccttg cataaacacg acaaagcgca gtccaggtcc
     7801 gtctactgaa ccgaaagttt cgatagaatg aacatagccg attgtttttt cttccattta
     7861 agttcacatc ttttctttga tttcatttta agtacaagtt agctgtcgaa gcgaagcttt
     7921 ctgaacaaaa tttttcgcaa ttgccgcttc gttatgtgta aaatttacag ggacggaaca
     7981 tacaagtcct gcccctgtaa atcgtttctt tcaaataagc atactgatta ttcgattttg
     8041 atgcgatggc tattcaacgt cctttcgacg ctaagtcgaa aaactttttt cacgaacttt
     8101 ttacattctg tcgtgtgaag ttctcatcaa gacatctaat tgttgttctc ttgtcaagtc
     8161 acggaattta actgcataac cagatacccg gatcgtcaag ttcggatatt tcattggatt
     8221 ttccattgca tcacgcaata aatcattact gaatacattg acattcaaat gataagctcc
     8281 ttttgagaag tatccatcta agacattacg caagttttcg attcttgtat cttgttcttt
     8341 tccaagacct gaaggattga ttgtttgggt atttgaaatt ccatccaatg cacagccgta
     8401 attcatttta gctgttgagt tcaggctagc taataaccca ctttgttccg cattgtaact
     8461 tggatttgct cctggcgcaa gtggttttcc agcttcacgg ccatcaggag tatttcctgt
     8521 atttttaccg taaacaacat ttgaagtaat cgtcaataat gaagttgttg gttttgagtt
     8581 gcggtaagtt ttatgacgtt tgatttgtcc catgaagtat tccaagatcc atgaaactaa
     8641 ttcatctgca cggtcatcat catttccgta tttagggaag tcgccatcca cttcaaaatc
     8701 aactgctatt cctttttcat tacggattgc ttttactttt gcatatttga tcgctgacaa
     8761 gctgtctgcc gcatgagaga tcccagcaat acctgtagca aatgtgcgtt gcaagtcaga
     8821 atccatcaaa gccagctgag ctgcttcata agaatattta tcatgcatat aatgaatgat
     8881 gttcaacgta ttgacatata gttctgctaa ccaatccatc atattcatga agttatgttt
     8941 cacttcttca aagtctagta tttctgatgt gattggacga tattctggtc caacttgggc
     9001 gccagtcact tcatcgactc cgccattgat ggcatataat agtgttttag ccaagttagc
     9061 acgagcgcca aagaattgca tgtcttttcc gacaactgtt cctgatacac agcaagcaat
     9121 cgcacaatca tcagaacccc attgttcacg aagctgatta tcattttcga attgaatcga
     9181 agaactttcg atagcgattc tgctagcata tgttctgaat ccttctggaa ggtgttcaga
     9241 atacaaaaca gtcaagtttg gttctggtga aggtcccata tttgttaatg tatgaagcag
     9301 acggaaatca ttctttgtaa ctaatgaacg accatctaat cccatacctg caatggataa
     9361 agtagcccag atagggaatc ctgagaataa ttggttatat tcaggtgtac gcgcgaattt
     9421 gaccatacgt aatttcatta ctaagtggtc gatcaattct tgtgcttctt tttctgtgat
     9481 tactccatta tccaaatcac gttggatata gatatcaagg aatgctgaga tacgtccgat
     9541 tgacatcgca gcaccatttt gtgatttgat tgctgctaaa tatccgaagt atagccattg
     9601 gacagcttct tttgcatttg cagctggttt agaaatatca tagccatatt tcgcagccat
     9661 ttctttcatt tgcgctaatg cacgatattg ttcagtgatt tcttcacgta aacgaatcac
     9721 ttcttcactc attgtatgat aaccagtcat atcatgatct tttttctttt gttccatcaa
     9781 atagtcgatt ccatacaatg ccacacgacg atagtcaccg atgatacgtc cgcgaccgta
     9841 agcgtctgga agccctgtaa tgattttgtt tttacgagca gcacgcattt cagcagtata
     9901 agcgtcaaat acaccttgat tatgtgtttt gcgccagtca gtgaaaatat gtgtcaattc
     9961 atcgtttggt gtatagccat tgctttttag cgcattgtta gccatattga ttccgccgaa
    10021 cggcatgaat gcttgtttca aaggtttgtc tgtctgtaga ccgacgattt tttccaaatc
    10081 tttatccaaa tagcctgcat cgtgtgaagt aatagttgat acgatttctg tgtccatatc
    10141 atagacaccg ttacgctcga attggatatc atttaacttt tgcaattcat cccataattt
    10201 ttctgttgct tctgttgggc cttccaaaaa gctgtcatca ccgcgatatt cagtgtagtt
    10261 attttgaata aagtctctag tgtctacgcc ttctttccat ttggtacctt taaatccatt
    10321 ccattgctcc attgtatgga cctcctaaat ttctgtttta tgtaacagtt tttacgtgat
    10381 tagtataaca cagcacccta tctttgcaag ggttttcttg ttttttattt aacaattttt
    10441 atgttttatt tacatatatt taaatcaacg ttgacatacc aacatttaag cattgtgaac
    10501 actttcatct ttttttgcaa acgtataagg atttattaaa aatgaatgaa aaagagacgc
    10561 cacctgtatt aatacagatg gcgtctcttc attaaaatac tacaacagtt acgactcgat
    10621 gaaaatctca ggagcatcga taacttcgat gacttctcca ccttttttct catccatcgc
    10681 aaatgatcca ttcgatgttc gatcacctat agggaaatca gttgggtcaa tcgttaactg
    10741 ctgccctttt tgattcatca aggttatttc ttttgatctt ccttccgaca tatagataat
    10801 acggtgcgga ttttttttca gctcacgcat gaccgtcact ccccgcttcg cacgtgaagt
    10861 ttggctgatt tcttgagcaa gcatgcgttt aacagctcct cgctgagtaa cgattacgat
    10921 cggcgtatca ccttctgcta acacaagcag cccgttgata atcctatctc catctttcaa
    10981 attcatagac ttcactccag ctgcctttgc tccaacaacg ggaacatctg ttaaaggata
    11041 gcgcaaaccc attccttgat gcgttactaa gaaaacatct aagcctggct gatcttcttt
    11101 cagatacaca gcaatcagtt catcttcttc cgcctttagc ttcatacagc tgagcggacg
    11161 gctcttatag gtgcgccatg gttcgaactc tgtcattcgt gtttgcttga tcaatccatt
    11221 cttgctgatg aatacaaatg ttttctctgc ttgaattttt tgatatggga aaacagctaa
    11281 aatcgattcg tctacagaca gattggagat tgtttgagaa atgtgctcac cagcatcttt
    11341 ccatttcaaa tcgggtagtt catggacagg gcggtagatc acatttgctt tatttgtgat
    11401 cagaagaaca tggtccaatg tactcaattc acctgagtat agcaagtagt cgccttctcg
    11461 catcccgatt tcttctggtt tggaagcact ataagaacga aggcttgttc gcttcacata
    11521 gccttctcgc gtcacactga caattacgtc ttcttgtgcc actaagactt gcgtatcaat
    11581 tttgatttct tcgatttcgt cttcgattgt cgttagacga gcggaagaat aatttttttt
    11641 cacttctcgc aattcttttt tcataacaga gaaaagctct ttgtcatttg aaaggatttt
    11701 attcaactct gtgatttgcg caatcaagct ttcggattcc ttttgaagct ctgtgatatc
    11761 cgtgttcgtc aaacggtata attgcaatgt gacaattgct tctgcttgtt cttctgtaaa
    11821 ttgaaagacg tcgactaaat tctttttggc gtcttttttg tctttacttt cacgaatcgt
    11881 tgcgattact tcatccagaa tcgataaagc tttcatcaat ccttctacga tatgctgtct
    11941 tttttgagct ttggctaaat cgaattgact acgttttgtt atgacttgct ttcgatgctc
    12001 gatataactt cttaaaatgt ctttcagtcc aacttgatgc ggagtcatat ggtcgatagc
    12061 gaccatattg aaattgtaat tgatctgtag ctcagtgttt ttgaacaaat aattcaaaat
    12121 tccttgagca ttggcgtctt ttttcaattc gacgacaatt tgcagacctg ttctatcact
    12181 ttcatctcgt acttcagcta ttccatcgat tttcttattc aaacggatct catccatttt
    12241 tttcaccaag gtcgctttat ttacttcata tgggatttct gtgatcacga tttgctgttt
    12301 attacccttc attgcctcta tctttgtttt cgaacgaaga ataacttttc ctcgtcctgt
    12361 ttcataagct tttttgattt catcttttcc ttgcaagatc cctcctgttg gaaagtcagg
    12421 tccaggaata tattccatca atttttcaag gctcgcttgt ggatgatcga tcatatagat
    12481 tgtgccatcg atcacttcag ctaagttatg agtcgggatt tctgttgcat atccagcaga
    12541 tatacctgtc gatccattga cgagaaggtt tgggtatcga gctggaagga ctgttggttc
    12601 tttttctgta tcatcaaaat tccacacgag atcgactgtt tctttctcga tatctgctag
    12661 catctctcca cttaacttgg ataatctggc ttcagtataa cgcatcgcag caggtggatc
    12721 accgtccata ctcccgttat tcccgtgcat ttcgatcagt acttcccgca acttccagtc
    12781 ttggctcatg cggaccatcg cttcataaat actgatgtca ccatgaggat gataattccc
    12841 catgatattt ccgacagatt ttgctgattt acggaatcct ttgtcatacg tattgccatc
    12901 tttgttcatg gcaaataata tccggcgttg cactggcttc aatccatcac ggatatcagg
    12961 cagtgcacgt tcttggataa tatatttgga ataacgtccg aagcggtcac ccatcacttc
    13021 ttctaaagtt aattcttgta cttcttgtcg attttccatc aggtgacctc ctattctact
    13081 tcaaataact gattgttttc atttttatct gctcgttctt catctaataa atcattagaa
    13141 actgaaggag agatttcagt atcctctttt ttatctaaga tactgccatc ttcttcgagc
    13201 gtaaattgca cgtgattttc gatccatttt cttcgaggtt ctactttgtc acccatcagt
    13261 gtggttactc gacgttcagc ttgcgcagca tcatcgatac ggacgcggat caacgtacgg
    13321 gaagtcggat ccatcgttgt ttcccacaat tgttccgcat tcatctctcc aagtccttta
    13381 tatcgctgca gcatatatcc tttgccgact tttttaatca ttgctgccaa ttcgtcatct
    13441 gtccacgcat attcaatgac ttgcttcttt ccttgtcctt tggatacttt atataaaggc
    13501 ggtaacgcga tatatacttt cccagcttca atcagcggtt tcatataacg ataaaagaaa
    13561 gtaagcagta atacttgaat atgggcgccg tctgtatccg catcggtcat gatgattact
    13621 ttgtcgtaat tacagtcttc gatagaaaac tcaggaccga ctcctgcacc aatcgtatag
    13681 atcatcgtat tgatttcttc atttttcaag atatcttgca ttttagcttt ttcggtattg
    13741 atgacttttc ctcgtaaagg tagaattgct tggaactttc tgtctcttcc ttgtttagca
    13801 gatccgccag cagaatcccc ttcgactaaa tacaattcat ttttcttagg gtttcgggat
    13861 tgtgcgggtg tcaattttcc tgaaagaaga gattcgcctt ttttacgctt cttcccattt
    13921 cggctttctt ctctggcttt acgtgctgct tctctcgctt ctctggcttt gattgcttta
    13981 cggaccaaca tctggctcat ttcgctattt tcctgcaaat aaaagcccat ttgttcactg
    14041 atgacgttat ctactacggt acgagcaact ggcgtaccaa gtttttcttt tgtttgccct
    14101 tcaaattgca gaagattttc aggtacacgg atagaaagta cagcagctaa cccttctctg
    14161 aaatcactac cttccagatt cttgtcccgt tctttcagca aaccaacttt tctcgcatat
    14221 tcattataag atttcgtcat cgccgctttc ataccagctt catgggttcc cccatctttt
    14281 gtccgaacgt tattgacaaa agataaaaca ttttctgaat aaccgtcatt gtattgataa
    14341 gcgacctcta cttcgatacc ttctttttct cctgaaaaat aaacaactgg tgtcaatgta
    14401 tctttttctt cattcagata atcaacaaat tctttgattc cttcttcgta atgaaaaatt
    14461 tccttgactg gttcttctcc acgaagatca gaaagagaga ttttaacgcc ttttagaaga
    14521 aaggcagatt ctcttaaccg ttcggctaaa atttcatatg aaaatttcgt tgtactgaat
    14581 attgtatcat ctggtaaaaa atggacagtc gtcccgtttg ctttttttgt ttttcctatc
    14641 tttttcaaag taccgtcagg tttaccgccg tttttaaatt tctgctgata ctctacgcca
    14701 tctctaacga tcgttacaat tagccatttc gataaggcat ttaccacact ggctcctaca
    14761 ccgtgcagtc caccagaagt tttgtaccct ccttgaccaa actttccgcc agcatgcagc
    14821 acagtaaaaa tcacttcaac tgttggtatt ccggaagcat gcattcctac aggcatccca
    14881 cgtccgctat ctgcaacagt tatactattg tcttcatgga tggtcacatc aatctcactg
    14941 ccataacctg ataatgcttc gtcaactgca ttatcgacga tttcatagac taaatgatgc
    15001 aagcctcggc tgtcagtgga tccaatgtac atccctggtc tttttctaac tgcctctaat
    15061 ccttccaata cttggatgga cgagtcattg tattcgttgt ttactttttt tgccaaggca
    15121 aacgctcctt caataatact ttgcggcaaa accgcaccac gattatcata ctagaaaagg
    15181 tcgtgaaaaa aaagagggta gacgagaaat caattttaat tatctcctaa ccgctttttc
    15241 gacattcccc tctatcgatc atctcttctt tgtggtcatt tttatttttg gttttctttc
    15301 aacttaccaa gttcaatctt aatacaacga tccattacga tatctgtacg tcctgctttt
    15361 cttagcatct cagctgcttc tttgttttca atccctaatt gtgcccagaa cactttagca
    15421 tcagttttca aaaaatcacc tgctatttca ggtaaaaatt cactccgtct aaatacatct
    15481 acgatatcga ttggatcatc gatttctgtc aaattcgcat agactttctc gcctagaatt
    15541 tcttcaccag ccaataatgg atttactggt atgattttat atccattgtc ttgcaatacc
    15601 tgtgcaacca tgtaacttgt tcgttctggt ttattgctca agcctacgac ggcaatattt
    15661 tttgcttctt ccaaatagtt gaaaatcgtt tcttgtgttg gattttttac agacatttct
    15721 gccacccctt gcttttcttt ttattccatc tattatactt gtatctttca ttttttcaat
    15781 tgttcctgtt acctattcat gctaaaataa aggatagttt tcaacttgaa agagggtaaa
    15841 aaatgaaatt gatcttatta cttattttag catatctttt aggttctatt ccttccggtg
    15901 tctggattgg caaattgttt ttcaaaaaag atatccgaca acacgggagc ggtaatactg
    15961 gaacgaccaa tacatttcgt gtattaggca aaacagccgg gattgttgtc ctgcttatgg
    16021 atattttaaa aggtacattg gctacttgtt tgccgatgat cttccacctt tctgggatta
    16081 accctctttg gtttggggta tgcgccattc ttggccatac atttccgatt tttgctgggt
    16141 tcaaaggagg aaaagctgtt gcgacaagtg caggaatgct tttaggattt aatcctgtat
    16201 tttttgttta ttcttgtatc atctttatcg ttagtttatt ttgtacaagt atggtgagtt
    16261 tgagcagtat gatcagtgca gtattgatca ctttatctac gatcgtcctt ccttatgtag
    16321 ctcctgtgat tttggctaag cccaattggt tactgacgat cattgccttt ttagtttctt
    16381 cctttatctt ttatcggcat aaagacaata tcaagcgtat cagaaaccat actgaaagtc
    16441 gaatcccgtt tggtttgaat agcagcaaga aaaaatcctg atactttttc attccaaaga
    16501 gataaaacgg gagtgtgaca aatcgatgtc acactccctt attacttaca ggatagcttt
    16561 tttacacacc ttggtaaacg gtgttccctg ctaacttctc ggtcacaacc tcttacttca
    16621 ctgtgatgga atattttgtc ttgaagattt catgggcacc tagttgattg atccctactt
    16681 tttccatcaa gtttccacta ctgttcaccg tgtcagcgat tccgcaccac ggttcgatac
    16741 aaacgaaagg cgcttctttt ggataaggag accagatccc tacataaggc atatctttat
    16801 agcttaaagt tacgctatgg gaagattttt cacttcgaat ggcgtacgca tttagacccc
    16861 ttgtttcaaa aaccaaggca tcttgctcaa aaagttcacg attcaaagcc agattcgtat
    16921 ttgtctgacc taacgtcttt tgatccatat cgatgaatgg gcctttcaat ggtaggcgta
    16981 atcttgattt caatggagaa aatgccagat agaaatcttc aaatgacaat ccttcttcaa
    17041 gaggtacatt gaaagcagga tgtccaccga tagaaaagaa catttctttt tcaccggtat
    17101 ttgccacttg ataatgaacc gtgatgccgt ctcctcctaa ttcatagctg atcactaatt
    17161 caaaatcaaa tggatacttt tctaatgtct ccggcgtact ccttaagatg aaactagcca
    17221 tttcttgtcc atgttcaatg acttcaaaat ccatatcccg agcaaatccg tgttgcccca
    17281 tttgatatgt tttcccttga taaacatact gatcatcttt taaacgtcca acaaaaggga
    17341 ataaaaccgg tgcatgtctg ccccaatacg ctggatcacc ttgccagata tactctaaac
    17401 catgttcttt tgatttcaga ctgatcaatt ccgcaccgtg ttcagcgatt tcggcgatca
    17461 gctggtcatt ttgaataata acagtcattg ccattcctcc aacttcgact tactttttaa
    17521 aatgggaagg ctggaacaaa catttctatc tgccccagtc cccttacttc taataactgt
    17581 gttttgctaa ctcgtcttta aaacgggaat tcaatacttt taaataagtt cccttcatgc
    17641 ctaacgaacg agattcaatg attccagctg attccaattt tcgtaaagca ttgacgatca
    17701 cagaacgagt gatgccgata ctatcggcaa tattagaagc agtcagacga ccttcatctc
    17761 catccaatgc ttcaaagata gcttgtacag ccttcaattc gctataagat aatgtgttga
    17821 ttgccatttg gacagccgtt gcactccgta catctgcttc gatgcttcga gacttctgat
    17881 aaaggatctg cataccgaca accgtagctc catattctgc taagactaga tcgtcatctg
    17941 caaacgattg ttccatacga gataagataa tcgtacccaa acgttcgcca gctccaaaga
    18001 tcggaacgac agtcgtcaga ccaaatggat atttctctct tgactctacc ggaaatgcag
    18061 taatatcact agtaattggg atattagctt cagttttcat caactgtgcc actgtatcag
    18121 tataagattc aggaaattgt ttctcaacaa acattgtttt gattcgttca ttgttcacat
    18181 cgtgacgttc attataacct agtaatactc cgtcacttcc tatgatatag gtgttgctgt
    18241 caagaatacc acctagtgtg acagccattt tatcgtaagg caaatctgct tgcatgtcaa
    18301 aagtattctt ttgctgcaaa agtgtgttta tcttgcgggt tttcgttagc aagtctgtca
    18361 ttgtcgtcct ccgatttttt ataaaatata gcgacttaaa tcttcatttg ccgcaattga
    18421 gccaagtttg tcatccacat atgcttctgt gatcgttatt tctcccattt gcatatctgg
    18481 tgcttcaaat aatagatctt ctagtagttt ttccaaaatg gtatgcagtc tgcgtgcgcc
    18541 gatattatct gtgtcacggt tgacatcgaa agcgatttga gcgatccgtt caattgcttc
    18601 ttttgtaaac gtaactgaca cattttctgt accgatcaac gcgacgtatt gttttactaa
    18661 agcattattt ggttcggtca gaatcttcac aaaatcttca gctgtcaaat catctaattc
    18721 tacccgtatc gggaagcgtc cttgaagttc tggaattaga tcacttggtt tagacaaatg
    18781 gaaagctcct gaagcaatga ataggacgtg atctgtttgg atgatcccgt attttgtgtt
    18841 tacttgtgaa ccttcgacaa ttggcaatat gtctctttgg acgccttctc tggatacttc
    18901 tccagattgc tgtgatttag aagtgatctt atcgatttca tcgataaaaa taattccact
    18961 ttcttccgct aatttgattg cctcactatg gatatcagca tccttgacta attttgctga
    19021 ttcttccttg atcaataact ctaaagcttc tttgactgtt accgtccgtt ccactttttt
    19081 cttcggtgtc aaagcaccta gagtttcatt taggtcgatc cccatttgct ccaatccatt
    19141 gttcatcatt ggtgcaggtt tcttttggtc ttcaacttca atggttactt cacgactatc
    19201 cagtaaaccc ttttgtagtt gttccaagac tgtacggcga ttgattcgaa tatcttctgt
    19261 cacttcttct tgcggttcag cggtattttg ctgactagca ttaaaaagat tcatcatttg
    19321 ttcaaattga ttagtggatt gtttctgttc ttttttgata ccaggtacca agatttttac
    19381 caaacgtttt tctgcatttt ttttagcact tgtgtataca cggctgtact gctctttttc
    19441 tacgatttgg atcgcatttt ctactaaatc ccgaaccatt gattctacat ctcttccgac
    19501 ataaccgact tcagtaaatt ttgttgcttc gactttgaca aatggtgcat tgacgatttt
    19561 tgccaaacgt cgtgcgattt ctgttttacc gacccctgta ggtccgatca tcagcatatt
    19621 ttttggtgta atatcttgtt gcattttttc atctaactgc aaacggcgat agcggttacg
    19681 caaagcaacg gcaacagatt tttttgctgc gtgctgtcct acgatatatt catctaattc
    19741 acttacgatt tgtcttggtg tttgttttaa ttcatacata tctactcacc tcttacagtt
    19801 cttctacgat aatattatga ttagtaaaga tacagatatc tgcggcaata tttaacgcat
    19861 tttctgcaat ttctttcgca ctcatgtcgt tttgagcata gtttttcatt gctcgggctg
    19921 cagccaacgc aaaattccct cctgatccga tagctaagat accatcatct ggtgtgataa
    19981 cttctcctgt acctgaaaca agaagcattt ctttatcatt cattacgatc agcatcgctt
    20041 caagtttttg catagattgc tgtgtccgcc attcttgagc taattcgaca gctgcgcgtt
    20101 gtagattccc gttatattcg tttagtttgc cttcaaattt ttcttctaaa gtaaacgcat
    20161 ctgcaacact tccggcgaaa cctacaacaa cttctccatt gtagatacgt cgtactttct
    20221 ttgcagtgcc tttcatgaca acagattcac ccattgttac ttggccatct ccagccatag
    20281 caaatttccc gtctttttcg acggcacaga tagtggttga atgaaatggt gattcgacca
    20341 tgtttgtttc ctcctaaatt tgttcttttc cttattttta cgctcttgga tggaaagaac
    20401 gatagttttt ttgtaaactt tcttttgtta catgggcata gatctgggta gtcgataaat
    20461 tggcatgtcc tagcaattct tggactgtcc gcaaatctgc cccattattt aataaatgag
    20521 tagcaaatgt atggcgcagc atgtgaggat ggatatcact atccaaactg ctttttttga
    20581 tcatttgatt caagacatat tcaattccag taggcgtgat tggttttcca tgatgattca
    20641 caaaaacgat atcatgctct tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga
    20701 aatattcttg cagagcatct tgagcaaaag aacctagagg agcatatctc tctttgttcc
    20761 ctttaccatg gatcaacatg acctctgaat caaaatcaat gtctgatacc gacaaactgc
    20821 tacactcact taaacggatg cctgacccgt aaaggacttc cagtaaagcg cggttcctca
    20881 aatccaaagg actatctcct gctacgctgt caaaaagtgc ttgcatttca ttttcataaa
    20941 aaaaacgtgg cagtctcaaa tttttctttt tgagatgaac gtaagaaaat ggattttctt
    21001 ttatcacttc ttctttgaga agatactgat aaaacgaacg taggctggct atcttgcgac
    21061 tgatagaatt tctgctatag ctaggacgat gatcagctaa tgcactcaaa tagacacgaa
    21121 ccgtaaaata ttccgccttc agcagatcag cgtctccaga ttcctccaaa aaatgtttga
    21181 aatggagaat gtcttcttca tatgcttctt tcgttttttc tgaatatcct ctttcgacaa
    21241 tcaaataacg caaaaatttt tgttcccacg tggcttctgt cattttctca ccttcctttt
    21301 gtcccagaga ataatttagc acagcgttta taaaaagaca aataaattgt cagaatttaa
    21361 ccaatatttc taagtttgtt cacaattggc gcaattcctc acattttgtt tgagaaattc
    21421 ttaagaaaaa cgcaagaaaa agagcgagac tatccgtctc accctcttca tggagtttcc
    21481 ttactcattt gggattgcag ttttttctaa ttctgttttt attgcctcat tagcttttaa
    21541 agctcgttgt gcaattgctt catatcttgc ttttttgtct cgaatgcgct ctggcagttc
    21601 aggaaacaga ccatagttgg cattcatcgg ttggaaatgt tttccttccg catgagtaat
    21661 gtagtaagcc atacttccaa tagctgtttc ttgtggaaac tcgactggtt ccatttcttt
    21721 tgctaagcgt gcggcgttga ttccagcaag tagtccgctc gctgcacttt caacatagcc
    21781 ttctacacca gtcatctgac ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt
    21841 cggtttcagc aattctggcg aattcataaa gctgtttcgg tgcatcacac catagcgaac
    21901 gaattcagca ttttctaacc caggaatcat ccggaaaaca cgtttttgct cgccccattt
    21961 caagtgagtc tgaaagccca cgatgttgta taaagaagct gcagcattgt cttgacgcag
    22021 ctggataaca gcataaggac gtttgccagt tttaggatct tccaatccaa ctggtttcat
    22081 aggtccgaaa agcatcgttt tgaatcctcg ctgtgccatt acttcaattg gcatacatcc
    22141 ttcaaaatat ttttctttct caaaagattt aagttctgcc acttcggcat tgattagtgc
    22201 ctcatggaaa gctttgaatt cctcttctgt catcggacag ttcaagtaag ctgcttctcc
    22261 tttgtcatag cgggacttca aatatacttt atccatatcg atcgtagctt tgtctacgat
    22321 tggtgcagct gcatcataga aataaaaacc atctgaacca ttgaatgctt tgatgctttc
    22381 tgctaatgga tgagaagtca aaggtcctgt tgctactacg gtgattcctt ccggtatttc
    22441 agtaatttct tcgtttatca ctgttactaa tggatgctct ttgacttttc gggtaattgt
    22501 ttcagaaaaa gaatcgcggt caacagctaa ggcaccacca gctggcactg ctgtttcatc
    22561 tgccgaagtg attactaccg aattcaatcg gcgcatttct tctttcaata accctactgc
    22621 attagccaaa ctgtttcctc ttaatgaatt ggaacatact agttcagcaa aattacttgt
    22681 gtgatgcgcc tcagtcgatt tgactggacg catttcatat aaacggacag ggactcctgc
    22741 ttgtgcgatc tgccaagcag cttcacttcc ggcaagccct gctccaacaa catttaccat
    22801 tttcaatgaa agaacctctt ttctattatt tttggatgtc ttcttcgtaa tctccattta
    22861 cacagactac ttgtttaccg cctttgactt tcttctcgac cagatatccg tcacattttg
    22921 gacaatttct gcccactggt ttatcccaag aagtgaattc acattctggg aaacgggaac
    22981 aaccatagaa tagtctgttt ttcttagatt ttcgttcaat gacttgtcct tcatgacata
    23041 ctgggcaagt cacaccgatt tcttttacga ttggtttcgt attccgacaa tcaggaaaat
    23101 tactgcaagc ataaaacttc ccatatttcc ctaattttat caccatcgga tgtccacaga
    23161 catcacaatc gaatcctgct ggttcatctt tgatttgaat tttttcgatt ttttcttccg
    23221 cattcgtcag ttctttttca aacggtttat agaaacgatc aaccactttg acccacgctt
    23281 ccgtgccttc ttcaatttta tccaaatctc cttccatttc tgctgtgaaa tggatatcga
    23341 cgatttgagg gaagaattca cagattaatg aattgacaat ttctcctagt tcagtcggct
    23401 caaatctttt ttgtgtcaat tttacatagt agcgtctttg gatcgtatca agtgttggtg
    23461 cataagtgga aggtcgccct accccgtttt cttctagtgc acgaatcaac gtagcctcac
    23521 taaaacgtgc tggtggttga gtaaaatgtt gttttggttc aatatcaaca gattggacta
    23581 catcgccttc ttcaagttct ggaagaatat tttctttctc ttctttgccg tcatctctac
    23641 cttccacata cacttgcatg aatcctttga atttgatttt tgaaccgttc gcaatgaaga
    23701 tgacaccatt ttgttctaat gtcactttca ttgtatctaa aatagctggt gtcatctgac
    23761 tagccacaaa acgtgaccag atcaatgtat aaagtttcaa ttgatcttta tctaaatatt
    23821 gtttgagttc atctggtgta cgaagcacac tcgttggacg cacagcttca tgggcatctt
    23881 gtgcgccttg agcatttttg atctttcggt gatcatgtct agaaaattct tttccatacg
    23941 tatcttcaat aaatgctgcg gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag
    24001 tacgcatgta tgtaatcaag ccgactgttc cttgtttacc caaagtgatt ccttcatata
    24061 attgctgtgc taccatcatg gtttttcttg ttcgaaagtt taacttacgg gcagcttctt
    24121 gttgcaggct acttgtagtg aaaggattcg cagggtttct tttgcgctct ttcttttcta
    24181 cttttttaac ttcgaagtct gttccatcaa ttctagcagt gacttctttt acactttgtg
    24241 catctggtaa tttctttttc ttaccgtcga ttccccagaa atttgctttg aatttcttgc
    24301 gagctttttt aaaattaccg tcgatgctcc aatattcttc tggaacaaat tctcggatct
    24361 ctttttcacg gtcaataata atttttaaag cgacagattg gacacgtcct gcacttaacc
    24421 ctttcttgac cttacgccag agaataggac tgatcgaata accaacgatt ctgtctaagg
    24481 tacgacgagc ttgctgtgca tctactaaat ctacatcgat cgttcttggt tccttaaaag
    24541 ctgctttgac tgcgtctttt gtgatttcat tgaagaccac ccgatttttt tctttcagat
    24601 ccaacccaag taggtacgct aaatgccaag caatcgcttc tccttctcta tccgggtcac
    24661 ttgccaagta aactttttga gctttttttg ctgcggcttt caggcttttg atgacatcgc
    24721 cttttccgcg tatagaaata taatggggtt cataattgtt ttcaaaatcg atacccattt
    24781 tacttttcgg taaatctctt atatggccaa cactggccat tactttatag tttcgtccaa
    24841 gatatttctc aattgtcttg gcttttgcag gtgattctac aatcactaaa tatttatacg
    24901 ccattactga atggactcct ttccattgtc gtgttcctac tattaaaaga ggattcatac
    24961 aaatcgtagc taatcatatc cattcagaaa acgtttgtca agaaaagcaa atgaaaagta
    25021 tggtgttatt ctttaaattc tgccaagata tcttcacccg aaaccgtgca aactgcaccg
    25081 tcttggatca aatgatgaca tccggtagaa tgttctgtta aaatatttcc tggaacagaa
    25141 aaaactgttc ttccaagatc caatgcttgc tgtgctgtta tcagagagcc gctttttttc
    25201 ttggcttcaa agacacatgt tcctttagac aaagctgcga taatccgatt tctcattgga
    25261 aaacaatgct ttttcgggcc ggattgagga ggatattcac tcaaaagaag atgatcattt
    25321 gcaatcacct cttgcaagtc acgattttca gcaggataga cttgctctat ccctgttcca
    25381 atgactgcga ttgtccgtcc accatgagag attgctgctt ggtgaccgta tgtatctatc
    25441 cctttcgcgc agccactgac gatcacatat ccagcttcta tcactggttt tagtaaataa
    25501 taaactgttt ttttggctaa agcagaagtg tgtcttccac caacaacgct taacatgtta
    25561 caagaaagta aagatagatc accattataa aacagtacaa ttggcggata ggtcaagtga
    25621 agcagctgag ggggatattt gggatcaaag caagtgataa acgactgttt cgattggatt
    25681 ttgccccagt tagcgtgaat ttcctgccaa cttttgcgaa acagctcccg gtgtttggtt
    25741 attttaccta tcttaatgat gtcttcaaat ccgatttctt tataaggata ctgcatcatc
    25801 cagttagcaa gtgcccattt tccttgacta ctgattcctt ttgtataagc aagtttcaat
    25861 aaattttctt ctatttgata catcataaaa ccgcctttcg tctctctttc taataagata
    25921 cgtaaaagcg gctgtttttt attgtaaata tttttttaca ggtgcaaaac ttttacgatg
    25981 gattggcgta attccttgtc gttcaagtcc ttctaagtgt tcctttgttc cataacctgc
    26041 attatgcgag aaaccatatc caggataaag cacgtcatat tctttcatca attggtctcg
    26101 atatacttta gcaatgatac ttgcagcagc aatcgacaca gatcgtgcat cccctttgat
    26161 tattttttcc tgctgaatag gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg
    26221 tacaatggac aatttttcta ctgcttggat catcgctaat ttacttgctt cataaatatt
    26281 cacatcatcg attgtctgct gatcaacgat cccgatcccg attgccaggg ctttttcttg
    26341 aacttgttga taaagaagtt ctcgcttttt ctctgataat tgtttggagt cattcagccc
    26401 taatatgatc tcatttttcg gcaagataac tgcagctgcc acgacaggtc cagctaatgg
    26461 tcctcttcct acttcatcaa tgccggcaat ccattggtga ccttgtgcgt acccttgctt
    26521 ttcaaagcaa aacatttctt catatttttc cttaattaat gcttcctttt tcaatcgtcg
    26581 ttcaaaagaa gccagtgctt gctgcacgcc tttgcgtgga tcatttctta atgattctag
    26641 tgtaggatca tctattctct gtatgctttt caatcgttct ttgatacttt ggatggattc
    26701 agtcatcact tttttgctcc atttcttcgc aacgatccag tgtgtatcgt ccaagttttc
    26761 cttgacgaat atcatgcacg actaattcgc tgccacgatc atagtcttct ttatacccac
    26821 gttttgctgt aatatccatc agtaattccg gtgccaattt ttcttcttgc tcgattgata
    26881 aaccataacg atcgctgatt cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa
    26941 tcgctaaatc atctaaatgg acaagctggt ctttgatcgc gcctgtcagc gctagttttt
    27001 tgccgatttc ttcatcttca aatttgggcc ataaaatccc tggtgtgtct aacaattcca
    27061 actcattacc taaacgaagc cactgctgac cttttgttac gccaggctta tttcctgtct
    27121 gtgcaatttt cttgcctact agtcgattca tcaacgtaga tttcccaaca tttggaatac
    27181 ctaagaccat tgcccgaata gcgcgaggct tgatcccccg agcagcatct cttgcccgtt
    27241 tttctttcaa tgcttcttta gccgcaggta ctagaccttt aattccttta ctttcttgtg
    27301 ccgtgatagc taatgcaaga tggccttgtt tacgaaaata ttcttgccac agctttgtct
    27361 gttgtggatc tgccagatcc gctttattca ataaaacaag gcgaggtttt tgctgaatga
    27421 tctgatctag cattggatta cgagaagata aaggtaatcg tgcgtctacc aattcaaata
    27481 cgatgtccac atatttcatt ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg
    27541 gaaaccattg gatggtcatt gttctctcca ttcctttcat accggaatat tccccggctt
    27601 tacttgtttt tttctttttc aatataaatt cactttttta ttataaagaa attccatcta
    27661 ttagttaagt gaaagtgaac cagtcctcat tctatcacat cttcactttt ttgatgatgc
    27721 aaatttttat tttggtttct atatatgaat ctaaattttt gacagcgttt cctaaataag
    27781 agatgataga cgaacaaaac agaaaggaga cgaaacaaat gaatagtatg gtttttgtta
    27841 attttcctgt tgctgatgtc aatcgttccg ttgcttttta tgaaaagctg ggttttaaaa
    27901 aaaatgaaga attttctact gaagaagctg gttcaatggt atgggatgac actttttgga
    27961 taatgcttct ctctcatgat ttttataaaa aatttataaa agataaagaa atagctgata
    28021 ataaaaaaac aagtggtgct ttgatttcgt ttagtgttga aaatgcggat gaagtaaaaa
    28081 aatttgctca aattgcaaaa gaaaatggtg gtactttcca cgaagtagat atgggaatgc
    28141 ctgaagaaga aatgttttca ttagaagtcc aagatctaga cggaaataca ttagaacccg
    28201 tatggatgaa gatgtaagca gataactaat ctgacgtttt ttataacaaa aaaacaaagg
    28261 cggatgatac gataagtatt ctccgcctct atttttttta ttcgtacaat ctctttttta
    28321 cagtttataa aagcagtctt ctcacccttt ttttaattgt tagatgccag tgtatgtaaa
    28381 tcaccaatgt gtcatgacac attggtgatt ttatggtatc cttttattat taactgacaa
    28441 aggagagaat attttgaaca ctttgattgc aaaagacgtt tttagattgc ccgaaatgaa
    28501 tgcaagattg ttgtcgacct ctgctctttt attagccctt gatgtcatct tatacaagat
    28561 agcgattggt cctgcttatt ttcaaatcac attcggattt ttaagtatgg cgcttatggg
    28621 ttatctatat ggaccgatct gggctggttt gcttgaaatg ttgtcgaaca ctttaaattt
    28681 cacgatattt ggttcaggaa cgtttcaagc tgcttttctt atcacggctt tccttggtgg
    28741 atttattaat ggcttatttt tatatcataa aaagataaac ctgccttccg tcatcgctgc
    28801 ccaattattg attatgatcc cagtcagcct ttttatcaat tcttggctga tgtctgtttt
    28861 gtttggtgca gatttcaaag taatttttct aggtagattc atcaggaata tgatccaaat
    28921 ccctattcaa ataatggttg tttattcatt tatcaaaact ttagaggcta gaaaagtgtt
    28981 taaacgagct gtttgatttt acgtactaag gtagtaaact gattttggat taaacaataa
    29041 agcgtgcagt ctcaacagac tgcacgcttt attgtttctc actggatcca ttttatttca
    29101 tcaagtggat aatagacaaa tcttgcttta cctagaatat cagttttatt gatcgtgcca
    29161 aacgaacgac tatcttttga aactcttcga ttatctccaa gtacaaaata attatcttcc
    29221 ggaagcttgg attgtcccgt tagctcttgt agtgtaaagt ccgtcgtata ggatgcattt
    29281 gcatgatctg atttcaggtt tttagttaga taaggttcct taactacttt cccattgatc
    29341 ttcaattgat cattttggta gctgacattt tctcctggaa gaccgattac tcgtttgata
    29401 taaatcgtgc catctgctaa ttgaaaaaca acaatatcaa atcgtctgat ctccgaaaat
    29461 ttttccatca caaccatatc accttgcttc aacacgtttt ccattgaatt gccttccact
    29521 ggtaccggga tgaataaaaa tccccgcaac atgaacgcaa ggaatacaga aacaagtaag
    29581 tatttgaata caagccagaa gcgattaata tagcgttgtt tttttgtcaa tctctatcaa
    29641 tccatccctc tttttttcat tctttcttta ttataagaaa aatatcaaaa aaatgcgact
    29701 agatagctga aataaaaata attctttcaa ttaggctatt ggacaaaaaa cttttttgat
    29761 cataaagtac aaaactatct cttatatgtc atgattattt tctaaatatt tttcccatgc
    29821 ttcgtcaaag atatccatgg acggcaaata gtcgacattc agttccagat aattagaaat
    29881 ttcttcgtag ctagagctgt gcttaggaaa ttggatgtct ttaccggctt ctgtagcgaa
    29941 aacagataaa gcatcatgtg tgccaccgcc tcgcagtgtc attaaataat gaaaaaagct
    30001 tcgattcacc cttcgttcct ccaattttct tcgataaagg cagcccggcg tgcatggttt
    30061 cttttaaaat tttcaggatg ctttttataa tactcttgat ggtcttcttc tgctagataa
    30121 aatggttggg ctggttcgat ttttgtaacg attggctgtt cgaaacgtcc actttcagct
    30181 aactgtttct tacttttttc agcaatgatc cgctgttctt ctgtcaaata aaagatcacc
    30241 gggcgataat tgtcaccgcg atcctcgaat tgtccgaaag cgtcagttgg atcagtttgc
    30301 tgccagtaaa gttctactaa ttgttcatat gaaatcaatg ttggatcata gataatctcc
    30361 actgcttctg tatgtccagt cgtttggctt tttacttgtt cataagtcgg atctggtaca
    30421 tgccctccag tatatcctga catcactgta tggataccag gaagcgtgtc gaaaggctgg
    30481 atcatacacc agaagcagcc tcctgcaaat attgctcgtt ccattatgat tccccctcta
    30541 atactactca tccgatgatt ttttattttc ttttgtcttg ctttcagtac tgctggtgct
    30601 agttttgttg gtatttgagt tgtttgaagc atcagaggta tcttttggca aatatagact
    30661 aaaccgaatg tcatcattta ctaagtcgat tttatctgct ttaatataca tcccgttttg
    30721 cattcggaat tgatccaacc taagcatgat agtcagatca tctgtattga cttcgatcca
    30781 atctggtaat ttatagtttc tcttgatcat attcatcaca tctttgattg gcaaactcaa
    30841 tgttccgata gaaagacttc tagcttttag ttggacattt ccgttttcca tcacatacgg
    30901 atcaaaatat aaatagaaat cgataggaaa acctagtact tcgaaagtac cattgagtag
    30961 tgcttcattt tccaaataaa atttatattc aatatcacta tctttttgat actcactaag
    31021 gaaaaagctg atgatttgat tgactttttc tttatttgaa ttgatcgtta agacaggatc
    31081 tccttgtctt tccgtaagag ctggtatttc tgggagttct ggttcccgat tagcaaaaat
    31141 acggctgcct aaaaatacgg tcgaaccaag tactacccca agaaggataa ggaaggcgaa
    31201 tttccagtaa tttgttttct ttgttttgtt tgaagatacg tcagatcttg ttttcatgtt
    31261 tattgatcat cctccttctc taaccattgg ttttgtgtct cgatcagctg atctctcact
    31321 gcatttgcca tcaattgata acctaaatta tttggatgga aatgatcttc ttcatagagc
    31381 acattatttt tgatgccatt tagattttct gtcgtgctag ttgtttgatc tgttccttct
    31441 tctgtgacac ctacttcctg attcaatcct tgataaagca aatcattgat tgggataaag
    31501 tagcagttcg gcattttttt tgtcaccgtt tctgttcctt tgttccaatt gttaacaatt
    31561 gtctgcatgt cagtgatctc aggaaagttc aaataaaatg gattgtagat gcctaaaatg
    31621 taaataggtg cggttttatt gagggatcga aattcttcca atagttctgt gacattttcc
    31681 tgatagtttt tcagtggctt tttaaaggaa ttgaccgtta acccgaataa attattttgg
    31741 ataactttca tcaaatcatt accgccaaca gtcagtgtga taaaatcagc tgttttgata
    31801 ttgtttcgaa tttcttcttt tgtttttact cgctttaaga tctggtcact tcgttcacca
    31861 tttacaccat agttttctgt ttcaaccgcg gtcaaattat aacggtcttt tagatcattt
    31921 gcgacaaggg gaacgaatcc tccttgcttg gtttgatctc ctactccttc agtcagcgaa
    31981 tctccgatag ctatgtagtg aagtttttct tttttatttg gatcttctct cacagtagtt
    32041 actggtttca ttagtgggcc agctttcggt atagaagcag ctaaacctgc atagatgatg
    32101 atcgcaatta ataatgggaa caaaacgagg atcaaatgtc gtttcgttaa tttcattttt
    32161 tcacctcttg tattcgattg gttctaatgt tatcaaaaat tttatcaaaa aggtagtctt
    32221 tatgaaaaaa gaaaacaatt attttcctaa ttttataaag aaaaagagaa taagctgcaa
    32281 gcctttcagg ctgagggctt atcctctttt cgactaatct acataataca tgattgcaaa
    32341 cgcgtcttta ccagtatgcg tagcgataac cggattcgta tgcagtacag ggatatgcat
    32401 gtccgggaat aattcttgca aaccctcttt gaattcttgc gccaattctt ttgcatccgc
    32461 atgtgaaata ccgatttgtc gcacatttgg caattctttc agttcatttt tcaaattatt
    32521 aaaccattta tcgaacgttt tctttcctcg ccccttagtt acagggatta attcagaatg
    32581 ggcgaaattc atcaccacgc gtatgttcaa aacactagac agcaaaccag ttgttcggct
    32641 gattcttcca ccttttacca agttatctag tgtggatatt ccgatgtata attttgtatt
    32701 gtcttgtact tttttgactt cttcaaggat tttctctact gttttacctt cttttgctaa
    32761 ttcagctgct cgaattacct gaaaagataa tccttggtca gtggtatcac tgtcgataac
    32821 cgttactttc gtttttgtta gattactggc ttgtcttgct gcttctactg ttccactcaa
    32881 gccttttgtc atatggatcg aaatcacttc actgccgtct tctcctagct catcatacat
    32941 cgagatgaat tctccaattg gtggttggct tgttttgggc agattttttg ctttcgccat
    33001 catttccata aatctttcgc cgtctagttg gtcatcatcg ggatatactg ttccgtcgat
    33061 catgatggat agcggcatga cacgaatgcc taaagcatcg cgtacatttt tttccatcgt
    33121 acatgaagaa tctgttacga tttttacatt tgtcattatt ttctctctct ctttctttaa
    33181 caagatgcct tattcatgtt aaactacatg taagacaatt tctattttta gtataacaga
    33241 caaataatga tttttcatta attacaccta gtaaaggagc cgttttcatg gaaaaaacga
    33301 aatttaatcg aacgtatgaa atattaaatg aagtgtttaa tgcagtcacc catggaattg
    33361 ggactggatt gagtatcgcg ggactagtgg ttcttttagt aaaaggtgcc cacttgcatt
    33421 cctccctcca tattgtctct tatgctatct atggatcgat gatgattctt ttatttttaa
    33481 cgtctacttt gtttcattca ttgattttta caaaagcaaa aaaggtgttc caagtattcg
    33541 atcatagttc gatttttctt ttgattgcag gaagttatac tcctttttgt ttgctgagta
    33601 tccgaggatg gctaggttgg actttatttg tattaatctg gttgtttgcg attcttggca
    33661 ttatatataa agcgttgacg ttgcataaac gggagagagt atcaaaagta tctacgatca
    33721 tctatatttt tatggggtgg ctctgtgtcg tggctgctgt tcctctttat cactctcttg
    33781 gaatgactgg tgttattttg atggtagcag gcgggctttc ttacacgata ggcgcattct
    33841 tttatagctt gaattctatc cgctatatgc atgttgtttg gcatttgttt gttatgttag
    33901 gtgcaggttt tatgtacttc tctgttttat tctttactta ataaaaaaaa taaagctatg
    33961 gatgggacag ctctaaataa cagctagtct atccatagct tttttatatc gtcaattcgc
    34021 ctgtcccgct ttttacagga ttctcattac gctgatacgt ttcaaattga tgcggataac
    34081 gatttttttc atccatttct ccttgactag tattgatcag tgaccaatca gaccagtcaa
    34141 caggtggaaa ataagcatct ccatcgaata catgatggat gactgtacgg tataatatcg
    34201 tacaaaatgg cagaaattct tgataaacag ctgaccctcc agcgatgaaa aagataccgt
    34261 ctgtttcttt ttccagagca agcacgtctt ccacactatg catgacatgc acgccttctg
    34321 ctttatagga agaatctctc gtcaaaacaa tcgtctgacg attcggcaac ggacggctac
    34381 ccatcccttc gaatgttttt cgtcccatca ctagtgtatt tgcttctgtc atttgtttga
    34441 agaatttcag atcatttggc aaacgccaag gaagacggtc ttcttttcca atcaatccgt
    34501 tttcatcctg tgcccaaatt gcagctaaca ttttctcatc ctttctgatt ctaaacagcg
    34561 ataggagctt tgatcgccgg atgtgggtca tatccttcta attggatatc ttccatctca
    34621 aaatcaaata ctgactgctt ttcctgattc aaaactaaag tcggaaatgc gcgtatttct
    34681 cggctcaatt gttctttcat ttgttcgata tgattgatat aaagatgagc atctcctaat
    34741 gtgtgaacaa attcgccaac cgctaaacct gtttcatgtg caattaggtg agtcagcaac
    34801 gcataacttg caatgttgaa cggaacgcct agaaacacat ccccactgcg ttgatacaat
    34861 tggcagctta gccgtccctc ttgaacataa aattgaaaca tcgtatgaca aggaggcaaa
    34921 gccatgctag gaacatcttc cggattccat gcagaaacga tcaatcttct agaatcagga
    34981 gtctttttga ttgcttcgat cacttgtttg atttgatcga taaagccacc gtcttttgtt
    35041 tcccagtgac gccattgata gccgtagatg tgtccaagat cgccgtatgt ttcagcaaag
    35101 gaatcatcgg ttaggatctt ctcgcaaaaa agcttgctct gctttttata ttcttcattg
    35161 aattcaggat ctgtcaaaca tctatggcca aaatcagtca tatctggtcc attatagtct
    35221 ggacttttga tgtagcgttc aaatgcccat tcatcccaaa tatggttttt attttgtaat
    35281 agatagcgga tattcgtatc tccttttaaa aaccaaagca attcgctttt gatcaatcca
    35341 aaaggtactc gtttcgttgt taacaaagga aacccttttt gcagatcaaa ccgcatttgg
    35401 tatccgaata agcttaatgt gcctgtgcct gttctgtctt ctttacgatg cccttcttcc
    35461 aacaattttc ttcctaagtc aagatacgct tgttccatcc tttttcctcc taatcttcta
    35521 cccattcact caagtattcc caccgtgcca ttttttcttc aagttgtgct tctgtttcag
    35581 atacgtcgtt ttgcaattct tgtaatcgag tgaaattatc tccttgatga ttcatctctt
    35641 cttggagcaa tgagattttt tcttcaagtt ctgcaatctc atcttcaatc gtttcccatt
    35701 ctttttgttc catgtaagat aatttcttct tttcagcagg ttccttgctc tgtatttttg
    35761 gtttcgcttt ttcagcaggc tgctgggtct ctttttgctt cagtaaataa ctggacatgt
    35821 ttccgtaata aagttcttgt tttccgtttc cttggaatac taataactta tctgctacct
    35881 tgtccaagaa ataacggtca tgggaaacag tgatcacagc acctggaaaa cttctaaaat
    35941 aatcttccaa aatcgtcaat gttgctatgt ccaaatcatt ggtcggttca tccaataata
    36001 aaacattagg ttgctgaatc agcagtttca acaaaaataa ccggcgtttt tctccgcctg
    36061 aaagtttgcc tatcagtgtc ccatgcataa atcttggaaa taaaaatcgt tcaagtaatt
    36121 ctgctactcc gatttgactg ccatctgctg tttttgcttc ttcagcagct tcttgtaagt
    36181 aagcaatcat ccgtttatct ggtgccattt cttcattttc ttgtgtataa tacgcaagat
    36241 gaaccgtttc accgatagac attgtaccgc tatccagggg aattctgcca gctagaatat
    36301 taagcaatgt cgattttccc gcaccatttt taccagtaat acctagtctt tcccgagatt
    36361 gaatcaataa atctaaatgt tctagcagtg tttggtcatt tatacggtaa ctgccatctt
    36421 tgatttccag tacttttttc cctaaacgtt gtgtcgctag gttcagctct aaatcatctt
    36481 cttggtttac ttggtacaag ttctccttta gatcttcgaa acggttgatc cgtgcttgct
    36541 gcttggtgcc acgtgcctgt acgccagccc tcatccaagc taattcctgt ttataaagcc
    36601 gcttgcgctt ttcttcttgt tctacttcta cacgatcacg ttctgctttt tctaagacgt
    36661 aagcttcata atttccttta tactcgtata attttccaaa tgaaagttcg aagattcggt
    36721 ttgctactct atctagaaaa taacgatcgt gggtaaccat caatagtgcg ccacggtatt
    36781 gtttcaaata gttttccaac cactcgatcg cttcataatc taaatggttt gttggttcgt
    36841 ctagtaatag taaatcgggt tcatcaatca acacttgcgc cagactaact cgttttttct
    36901 gaccacctga taaagtactg atttttttat ctaattcact gattcctaat ttttgcaaaa
    36961 taatctttgc gttggtatct gtagtccaag catcttcttt attcatttgt tcttctgctt
    37021 tagcataacg tttttgcaca tcagggtctt ctccgttttc ccctagttcg atcaatgctt
    37081 cctcatatgc tcgaacagtt tggatcaagg ggctgtttcc ttgaaagact gcttggagaa
    37141 cagttaattc atcggaaaat tcagctgctt gagaaagata accaatacgg taatcagttg
    37201 gataaaacac tgtttgccga tcaccgtcgc tgctatcgat accggcaata atatttaaga
    37261 gacttgtttt acccgtcccg ttcgtcccaa tcaacccgat acggtctttt tcgtggacca
    37321 aaaaagaaat ctggtcaaac aaatcttttt caccataagt ttttttgagg tcagttactt
    37381 ttaattcttt caacatgttc cctccttatc ttggatattg tatcataaaa atagtgaact
    37441 tcaggaaaat ttttttggta tttgttcttt ttgtgttctc aaaaaaagaa caggaccgat
    37501 tcgatcctgt tctgatatgt tttgtttttt ataaaacttg aaaattttta cctttttatt
    37561 ttattggact cgtttttttt gactttgctc gaaacgcaaa tcggaaagaa aagttatcag
    37621 atggtttgtt catcgtataa gattctaaca aattgatatt cctgcgatag ctgttcgcag
    37681 aaaacgtcgt gattttttca gcaacttcgt attcatcaat gatttttctt tcaagataat
    37741 aacctcttag catttttttc gtaaacagac tatcttgctg gatcatccac gaagaaatca
    37801 aattccccga gttcattttt attccttcaa gtttgcgttg ctggataaaa aattgaacca
    37861 tcgtttcatc ataaatatca gtcaagttat ctaaactttc aataatcaac tgagtatttt
    37921 tttgatatgt ctctctgatt tcttggatat cttcatcact aataaatgtc tgacgatcag
    37981 catatcttct ttgccaaaac attttcggat ggagaacgat tcgcattaac ctacggacaa
    38041 caatgaacca aaagccgata aaagacaaaa tctggcttgt gatagaatgt tcgaatcgag
    38101 aaagaaaacg ggagtaaaaa cgataaccat tagaagaaag cttgcccgaa cgataacttt
    38161 catccaatcc atcccgttcg atcgacaaaa tcagtgcttg tatttcttgt acttcttgct
    38221 tttcatcatc ggttaaatct tccaagtaca gatcacgtat ccgttcttga taattttcta
    38281 tcactgcttc agtggctaaa aattcttttg tattacgatc aatttccttt atttcttttc
    38341 gcaaaacttc aacgacctct tctaaaatca tcaaagcatt caaatccgtg cttttcgctt
    38401 cctctgtttc cgtcagcata ggcaagacaa tgatccctat gaccaaagtc actaagatga
    38461 cacccgccgt cagaaacaaa agcaaagacc gctggtaaaa catcatatta ttgattgaaa
    38521 atggcaaaat aaaaatcgta gccaaactga ctgttccctt tacaccgcca aatgttaaga
    38581 tcaagatctc gttcaactgt tttttgaact ttttactgcc atctttgaat acataaaaaa
    38641 gactgataga aatgaatctg atcacaaaaa gcatgacaga gattaatacg ataatcgcta
    38701 ataaaagacc gtttggatat aaaccgtctc cccatactgg cgagaacact tgcgtcaatt
    38761 cgattcctaa aaatataaat actaatgcat tcagtgtaaa agcaatagtt gtccatgtac
    38821 tgtgggacaa gctcgacaat tctgcatcaa acacagagat tttcctaaaa ccagaagctt
    38881 ggagaatacc tgctgccact gcagcaatga ttccagatac atcaaatgcc tctgccaata
    38941 cataggctag aaaaggaagc aaaagttcga tcagcagata ccctgtgaca tcttgtgctg
    39001 atgctttttc gatcaaatga attatttttt gtttgaacca gacaagcaaa aagccgatca
    39061 aagctccccc aatactagaa actactagac tcatccccgc gtttactgct gaaaaagatc
    39121 cggtgatcag cgcaccaaga gcaaactgga aggcagtaac accagatgca tcattcaaaa
    39181 gaccttctcc ttccaaaata tgcatcgcct ttttagggat attcactcgt ccagaaagag
    39241 aactgactgc taccgcatct gtcggaccta acgctgctcc aaaagcaaag caggcagcaa
    39301 gtgggacact tggcaacagg aatgataacg tcgcacctac cccgacaagg gtcaaaatga
    39361 ctcctccaaa tgccaagaac aaaattgtcc cgaaattttt gagaatcgaa gaaatatctg
    39421 ctttttctcc ttctctaaat aacaatggtg caataatcat aactaagaac aattcaggtt
    39481 caaaatcgat tgattgtccc cactctgtca gacctaatac cactccaagg aaaatttgga
    39541 tcagtggagc tggaatcatt ggcaaaatgc ggctagctat gttcgataag gtgatcgtta
    39601 ctgcaaatac aataactaac tcagcaaatt ccatcgtcca tcccctttat tttttatttt
    39661 gtgtaagcat ttttaaaaat tgttcttttt tctttaaaat tttggtttcg actacttcaa
    39721 cctgtgtttg gttcccctct aattcacact gtacacgttt tgactccaaa cgaagcagat
    39781 catgtttcag acgttccaac tgacgataca tcaatatatt tcttttacgc aacttacaaa
    39841 gtatttgacg gcggtcatcc atcgaaaggg cgccatagcg atcatgcaac agtttttttt
    39901 gttgttcaaa actctctctc attttccaga tctcctatac acaaacttcc atcattatag
    39961 caaagttctt tttggatgaa aaatatttca ttgtttcatc gtttattgct agttagaaat
    40021 ttccataaat ttttcaacgc ctcattctat tccaaatagt atcagatatc aagtaaaact
    40081 taagaaagat aaaagtttct gacaaaagga tgtttacttt cccattcctg cctttcttgt
    40141 tcttttatca caacaaaaaa atggggctgc gacaaaaatc ttgtcacagt cccttattct
    40201 gaataaacgg tgtggaagag acagcctatc agtagtaata agacacatct ctttcacagc
    40261 ctcatttttg cgtaaaaatc gatttattcg aaaaattccc cgtatacatc ttctacacgt
    40321 tgatctggat tgatcacgat atacaagtta cctgtttttt cgctgatttt tgttgattga
    40381 tattgattgt ctaatccttc tgttccttta tctagatatg ggaagtacac aagatcaggt
    40441 gtgccatttc ggtaaaggat ttccattcgc tcgatatctt cgtatttcaa tgcgcgatta
    40501 aacatgccca tttccagtcc gcctaggttg atatcatgtg tcgcaactgc gtccccttca
    40561 tggaagattt cgattttaaa tccagcacac ggatggattt caacaaattc actgccattg
    40621 attcgtccaa aacttgttgt gatttgtttg atccaaagat cgccgatatg acggcggttg
    40681 atcgtccagg tttctccatt tctaaaataa aattttagcg ctgtcatttc tttactcatt
    40741 aaaaagactc ctttatgatg taatttattc ttgtactacc tcagtttagc acaaatttat
    40801 gtaagtggtc acatcttatg ttttttcttc taatgacgta tctgcccact ttaagtagcc
    40861 aacaaagctg agcagataca gtctagtgtt ttttaaatca atttttcttt cgataaaaaa
    40921 tccaaaacct gttttttatc attttttatt ctttgttcta cgacggcttt ttcagctaat
    40981 tgcaaagctt cactaaccca cttaccaggt ttttttccag tttcttctag caagatttta
    41041 ccatctactg ctaattcgct taatgacttg ataggtaaac gatcatagga ttctcctacg
    41101 gcatccatct gaggtgtttc gtcaaaatag atcatcaatt cttctacttg cagtgcatgt
    41161 tttttgccta agaagtacaa ttcgaatggt tccaaagctc cagtttctcg ttgtcgtagt
    41221 ccttggacaa gtgcttgaac gtctttaatc atttggttag aacacttcca cgatttcaaa
    41281 aaggaacgaa tctcattttc ttttagatcc agttggctga tcaataatgc ccatgcttgg
    41341 ctctcttctt tcaatgggac ggatggtaat tcagcaaaac gtaaaagtgc ttcgccttct
    41401 tctttcaaac cgggacagca aatgtagcac tctgtttcaa taaatgcatg gattcccgcg
    41461 gcccgttcat ttcccaagag catcttgatg aattccacat ttatacgttc tacagatatt
    41521 ttacctaata gctcgtggtt ttctttgatg gcgtagaagg tttcttcctc taatttgaac
    41581 cctagttgac tgacaaaacg caacccgcgc atcattcgta aggcatcttc atggaaccgc
    41641 tcatgaggat tgcctacagc tcgcaagatc tgttcttcta gatcttgcag accatcgaac
    41701 aaatcgatga tttctccatc ttctttcaaa gcaaacgcat tgatcgtaaa atctcttctt
    41761 ttcaaatctt ctgccaacga acgaacgaat tcgacatgat ctggtctacg gtagtcctga
    41821 taagttgatt ccgtccgaaa tgtggtgatc tcatattgtt catcattttc taataccaac
    41881 acagttccat gttcgatccc aatatctatc gtttttggaa aaagctgttt gatttcttct
    41941 ggaaatgcgc ttgttgcgat atccacatca tggattggct ggttcagcaa gatatcacga
    42001 acacttccac ctacaaaata agcttcatat cctgcattct ctaatttttt cagaactgga
    42061 attgcctttt ggtattccag aggtaattgt tttaatttca tttttccaac ctttcctgcg
    42121 tgtttcttgt cctgagataa agactcagtt gcatggtgac tgtttgacaa gtccgtttca
    42181 gagtcttcaa ggccaggacc acttcatctt gtttactcgt ccttacgctc aaagcgatta
    42241 tggtctcttt gattgaattt ttccatgttt ttttcaaaaa cttcagttaa gtcgatattc
    42301 aatgaattag ccatgatcat cgtaacgaat aaaacatcac caagttcttc tgctaccgag
    42361 ttaactggtt cagaagcctt tttgcttttt tctccataag tgtggttgac ttctcttgcc
    42421 aattcaccta cttcttcagt taatcgagcc atctgagcta atggagagaa ataccccacc
    42481 ttgaattgct ggatatattc atccacttcc tgctgcatac tgtataaaga acgcttttta
    42541 tccatctttt tccctccaaa actatcttac caaaacttta acgcaaatcc tagataagag
    42601 caattgagaa aatcgaattt ccatgctaca tttaggaagg aatttctaaa tggaaattct
    42661 gtgaaaatac ataattgaaa gctggaattc atatggtcaa acgaaaaaca gtaaaagaag
    42721 ttctttttat cattatagga acaagtattt atgcatttgg tcttgtttac ttgaacatcg
    42781 ctaatcattt agcagaaggg ggagtctcag ggatcacctt gatcttaaga gctttatttg
    42841 gtattgatcc agcttatacg actttattaa tcaacatccc attgatttta ctaggaggaa
    42901 agatcctcgg taaacgttcc ttggcctata ctgttctagg tacactttct ttgtcattct
    42961 ttttatggat ctggcaaagg gttccactag aaatcaattt acagcatgat ctactgatcg
    43021 tttctctact tgcgggttta gttgcaggtg taggaagcgg gatcgtctac cgcatgggtg
    43081 gaacaacagg cggcagtgat attatcgctc ggattttaga acaaaattac ggcatcacta
    43141 tggggcgttc cctattggca tttgatatca tcgtcttact tgcttcatta acctatatcg
    43201 acttgaaacg tatgatgtat acgttgattg tttcgtatgt tttcagtcgt gtgattgatt
    43261 cgatcttaga tggcggatat tctgcaaaag gtctacttgt cgtctccaac aaaagcgaag
    43321 agattgcacc actactgatg accggtctgc aaagaggtgt gactttcctt tctggagaag
    43381 gcgcattctc tggtaattca aaaaaaatca tctatatggt cgttagctct agtgaattaa
    43441 ctgaagctaa gaggatcgtc cacgagattg acgaaaaagc tttcctttca atcatcaatg
    43501 tgcatgaagt ggaagggcaa ggctttactt atttgaagcc acagacaaga ttactgaaaa
    43561 aactataaga aaaaagacaa agataggcta tgaggaaaga ttccacatcc agctgacctt
    43621 tgtctttttt agtactatac tatttttaac taattgttat gttcacttac ccggttactt
    43681 ttcttttgtg tctgcaaaag gatctttcgg ttaacttcat cagataattt caaaaaagca
    43741 tccctatctc catcttctag tgcttgatcg atttgagcat aaagcatttt tagttttgcc
    43801 tcttcttctt tttcttgaaa atatcgttca attaattcag tatcttcttc actgactgaa
    43861 gaatcccaag aagaaaatgg attgtcttct aagatagata gatattctct tgtctgccaa
    43921 gcattttcga atatacattc cacatataaa ggctttttcc aattcaaacg gatttcatga
    43981 aaaatctggt ctgtgtccgt aaattctttt ccctgcaaaa acaatttcat gggttcccct
    44041 tcaacagata gatcactgat ctgcagtcct cgatccgcaa aattcgcccc ttcaacaaag
    44101 tggacattgc ttaaaatcgc ttcatgattg gcaagataat tcaagatcca aaaaacttct
    44161 cttcggctaa aagatacatg attgaccatc cacgttaaaa aattcttctt ttcccgtaca
    44221 tcaattagca tttttctcac ctctctgtta ggtcatctaa taatgacagt gcctccatat
    44281 ctcctggttc aagtgttaaa taataagtca ataattcttg cgctcttttc agctgccctt
    44341 cttctcttaa gaaaaaggca tactctttca agaaatctgg ttcatgtgat agttcatgat
    44401 aagcctgttc atagtgtaca gctgcaacgg taaagtcttc taattcatta taagcatggg
    44461 ctaggttcca ttctgcataa ggattagcag tttcttccat ctgattgatc acttgtatca
    44521 catcttcata tctttcatca tccaagtaaa gattacttaa agtaagtaat gtttcatctt
    44581 gtttgtctcc tacttctaaa gctttcagta aaagttcttc tgctcgctgt ctatcatgca
    44641 gacggaaagc attttccgag gccaggtgat aaagttcgac ttggtaagga ttttctttga
    44701 ttccttcttc taacactgtt tgtgcttctt caatcatttc ttcttcttga agagcttgtc
    44761 ctagatatag atataacgat tgataatgcg gattaacttc tcgaagttct tccagatagt
    44821 tgattgcctg ctgattttct ttcaattgaa gattcgtaaa agctagctgg aacagtcgat
    44881 catctgtcct ttcttctttc agtgcttctt ctaaaggtac gatagctgct tcgaattctc
    44941 cttgcatact taaggattgt cctaaacgtt cctgcatcga gattcctgaa atttcattta
    45001 tcccgcttgc caacaatgtt tcataaacca ctgctgcctc tttgaaacga tcaactgaaa
    45061 aatacaattc agctagcgca aactggatca atggttcatc aggaagcaac ccagctgctt
    45121 ctttcaattt tgcttcactc acttcaggga ttccgataac ttggtacaaa tcagctatcg
    45181 ccaataagct ttgcggatag taatcacttt gcttatccac tttttccagg tattcaaaag
    45241 ctaagtcgat ctcattatct tcgattgcaa tttctgctaa cgggatgttc agtccgtcat
    45301 tttctgggta ttggttgatc agttgttcaa aaatctgttt tgcttcgatc aaaaagccaa
    45361 tagccagcaa ttcttcgcct agttcagcta atatatctgc tgggtcttct tttaatgctt
    45421 tttctaaaaa gatttgtgct tgtgacaagt cttcatctga taaagcttgc aacatttttt
    45481 cgctatttgt attcatttca attccctctt atctcactga cgatttgttg aaataccgtc
    45541 attgttctca ataattcttt ttcgtttgga tgttggtatc cgtctattat acctattttt
    45601 tctaaaacaa gtattttttt gcttttttct gccaaaaaaa gaactttttc caaatactcc
    45661 atctttgaaa tctgttccag ccagtggatt ggatagccaa gcaagtccag ccacacaaaa
    45721 aagtttttta gttggaaatc aaaaggatat gcttggagat tccacacgaa atgaaacatc
    45781 attcctaaaa attttttcat actagcagaa agtagatggc cattttcggt cagataaaaa
    45841 gcttgttcaa acagtttacc ataatttgac aggttttctg cctctaattg ataaaaatag
    45901 atcatttctt cgatcatccc ggcaaacggc cgagtcgtta attgcttttt atttggatag
    45961 tttttataca gattttgcaa aaaacgatga tcacaaatca ttccgcaaca gataaaagtc
    46021 aaaaagtcaa tctgctttcc gtccacctgc cgctcgctga ttgtttgatc ataaacgatg
    46081 cgttctggca gattgatggt ttgcagcact acacgatttg gtagctgcaa aatcgtccgc
    46141 tgcggtgtta atgctcggct tagagaacgg atcgatacag ggatcatcca tagtgatgaa
    46201 tcaagggttg tatgtttttg aaaaaaacct gccagatcca tgatcccttc atttccaaat
    46261 ccgatgataa acatggattg attttcggga tagcgtttga cgaaatcaag caggttcttt
    46321 aattctgtta aatgattgca ttgagtgttg gcacaaatat accagtcatt gtttgcttga
    46381 tttgcaaata acgggttgat cttatctgca aacaaatcat aatagcgctg attcgttaaa
    46441 aataagattg gctgatcaat ggaaagttct cttagctgac tgactagcgt ttctccgtaa
    46501 aacactttcg tcaacgcatc ttctacttga taatcaagtt ccatacgcat ccctcctctt
    46561 cttattttta ttttaccatt agtaagcaaa ttctagaaaa gataagagca aagctcacct
    46621 cataacttgc aaataaataa aaattaaata aaatacatgt gttttctatg tatgacatac
    46681 ggattcggaa tctctgtctc ttatttattc cttttctgct aggaaataaa aagaaaagca
    46741 cttgtagccc tagagccaca agcgcttttt caactgtctt atttaacagc gtcttttaat
    46801 gctttacctg gtttgaatgc aggtactttg ctagctggaa tttcgatttc ttcaccagtt
    46861 tgtgggttac gacctttgcg agctgcgcgt tcgcgaactt caaagttacc aaaaccgatt
    46921 aattgaactt tttcgccgtt agctaaagca tcttgaatag ttgaaaatac agcatctact
    46981 gctgcagttg catctttttt agttaaatca gtagctgaag caactttttc gattaattct
    47041 gctttgtttg ccatggatag attcacctcc tccacaagag atatgataga aatatcattt
    47101 agttaaaact ttctgagtgg tccagaagtt taaaaagaaa tattgattaa cttcaatatt
    47161 ctgttatcac gatatcatat gaaccgctta atagcaaggt ttttaccaat tttcacatgc
    47221 tttttttagt catagaaacg taacaaacga aactgtcttt ttcttttgaa aaaaaaacgt
    47281 cgatatggta aaatgcctat tttctcctac gagggataat tcggatggac gtcccttcaa
    47341 aagtaaatgc ttttctgatt tgattttcta agaaacgttc ataagaaaaa tgcatcaatt
    47401 cctcttcatt gacaaaaacg acaaatgtag gtggtttgac agcgacttgt gtagcataaa
    47461 aaatcttcag gcgttttcct ttatctgttg gtgttggatt gattgctaca gcatccataa
    47521 tgatatcatt caacacagcc gaagagatac gaagattctg gttcatactt acttgttcga
    47581 tcatttcagg cagacgttcc aaccgttgtt tagtcactgc agaaacaaaa acgattggag
    47641 cgtaatcaag ataccggaat tcatcacgaa tctcttgttc gaaatcacgc attgtattgg
    47701 tttctttctt tacaagatcc catttattca cgacgataat aattccacga ccagcttcat
    47761 gggcgtaacc cgctactcgc ttgtcttgtt cccgaatccc ttcttcagca ttcagtacca
    47821 tcaaaacgat atcagaacgg tcaatggcac gcattgcacg catcacgctg tatttttcag
    47881 tattttcgta tacttttccg cgtttacgca taccagctgt atctatcatt aagaatttct
    47941 gaccactttc agaaacgaag tgggtgtcga ttgcgtctcg ggtcgttcct tcaatatccg
    48001 aaacaattac acgttcttct cctaaaatcg cattgatcaa agaggattta ccaacatttg
    48061 ggcgaccaat caaactaaac ttaatcgtgt cgtcgtcctc ttcttcagtc tcttcagaga
    48121 aatacttcac tgcttcatct aaaatatcac caatccctag tccatggctt cctgaaacag
    48181 gataaggatc gcctagccct aaggaataaa attcatagat atcgtttctc atttctggat
    48241 tatctacttt attgactgct aaaatcactg gtttattact tctgtataaa attttcgcaa
    48301 ccatctcatc tgcgtctgtc acgccttcgc gtccgctgac tacacaaatg attacatctg
    48361 cttcttcaat cgcgatttct gcttgatgct tgatctgatc cataaaaggt tcatcgccaa
    48421 gatcaattcc gcctgtgtcg atcacgctga attctcgacc taaccattca ccttttgcat
    48481 aaatacgatc acgagttact ccaggagtat cttcgacaat cgagatacgc tcaccagcga
    48541 tgcggttaaa aatcgtagac ttaccgacgt tcgggcggcc aacgattgca attgttggat
    48601 ttgccatatt atttcctcct cacaagtttc caatctcact tagtttaacg aatgcattgc
    48661 caactttcaa gcatagattg tcaattaaac gaagtatttt tattccttat tgaaatacta
    48721 atcgcttttc aaaagaaaaa ctgagacaaa agcagctgct ttcatctcag tcacagatac
    48781 aaaatcaatt atttttcaga atcatcggtt gctttttctt ctacttcttc tttcaacgca
    48841 tcacctaaaa tgtctcccat agtaaatcca gtattttctt ctggtaattc ataagactca
    48901 acattttttg gttcttcttc tgaagctggt ttttcttcta aagctttgat actcaatgca
    48961 atacgatgtt cttctggatg gatctctaat actttcactt tcacttcgtc accttcatga
    49021 agcacttcat gtggtgtcgc aatatgttta tgtgaaattt gagagatatg gactagccct
    49081 tctactccag ggaacacttc aacaaaggca ccgaagcttg tcaaacgttt aactgttccg
    49141 tctaatactg tgccaactgg agcttttgta tcgatttctg accaaggtcc aggctgtgtg
    49201 tctttgattg acaaggaaac acgttcttgt tcaggattga tagacaacac tttgacattt
    49261 acatcatcat tgacagccaa tacatcactt ggttttgcta catgactatg tgaaatctct
    49321 gagacatgga ctagaccgtc aattccgcct aggtcgacaa atgcgccaaa atcagtaaga
    49381 cgtgcaacac gtccactgag aacatctccc tcatggatag ttgaaagaat ttcttttttc
    49441 ttttcttctc tttcgttttc cactaccgct ttatgcgata agatcaaacg attttctgat
    49501 ggttcgattt cgatgatttt aaagctcaat gttttgcctt tatagtctga aaagtctgca
    49561 acaaaatgat cttccaccat tgatgcaggg acaaaaccac gtacaccgac gtcaacgact
    49621 aaaccgcctt tgacaacatt tgtaacaggt gcttcgatga ccttgccctc ttggaaatct
    49681 ctttcgatat cttcccaaac ttttttagca tctaaacggc gttttgaaag tagatagctg
    49741 ccgttttctt tatctttgcc gatcgtactg ataacgacta aatctaatac atcaccgact
    49801 tttactactt cgctgatatc ttctaccggc aaagttgata attcttttgc cggaacgact
    49861 ccttcaactc cagccccttc gattccgaca atcacttgtt tgtcttcaat gactaaaact
    49921 tcgcccttaa caacatcgcc aacttttact tcttgtacgc ttttcattgc gtcttccatt
    49981 gactggttgt tctcttgatt ttgatcaaat tctgtcataa cacttgtcct cctaaccaac
    50041 attctgccgt aagtacggta ctgtttttag tttaattgat tattcaaaaa aaataaagcg
    50101 atttcgttcg ttttccttga aaaaacaatt gtttttcatt ttttttctta atttaaaaaa
    50161 gaagataacc ttttttcaaa acaacagctt ttattgctgc aacgacttct ggaatcgatt
    50221 tgccagtcgt atcgatacga acagcatcct ctgcttgctt cagcggagag acgtcccttg
    50281 ttgaatcata atggtctctt tcctgtatct ctttcgtcag tgtttcaaga tcagtcggaa
    50341 ttcctttttc ttggttctct ttgaaacgtc tttctgcacg ctcacttacg cttgctacta
    50401 aaaagatttt cacttctgca ttcggtaata cagctgtccc gatgtctctt ccgtccatga
    50461 caacgccacc gcgttcccct atttttcgtt ggacagcaac taattcttct ctgacttttc
    50521 catgtgcaga gacttcagaa acagcttttg tgacatctgg ttgacggatt tctaaagtca
    50581 catcttttga tgctatgaat acatgctgtc catcttcttg ttgcgaaaaa gagataggat
    50641 actttttgat cagcgaaact agcccttctt catctgaaaa agatacattg ttcgtaatag
    50701 ctaaataagt tactgcgcga tacatggcac cggtatctgt ataaatataa ttcaattctt
    50761 ttgctaatat cttggcaacg gtacttttcc cagatgaagc aggcccgtcg attgcaatac
    50821 tgatttgtgt catgatataa ctcctttttc tcagaaaaaa agccgcaaag cgcggctatt
    50881 attatttaat tcttaattct tgaccagggt aaagcataaa gttgtttgga tctaagccat
    50941 tcaattcaaa caattgatca gtggtgatcc ctgcacgctc agccacttgt tttggacctt
    51001 cgcctgcttg aacagtcgtt gttgaaccgg ctgcagctga tgaagaaggt gttgtttctg
    51061 ctgcattgct gcttgaatcc cctgcttgat aagttgcctc acttgaggaa gtcggtgttg
    51121 tctctgctgc aatactgctt tctgacgtta cacctgtatc ttgagacaca gtagattctt
    51181 ccgcgattgt actagattct actgttgaag tctcagttga ctcctctttt gttgtacttg
    51241 tagaactttc tactactgat gtcgaagttt cagaagaagt tgctgctgtt gcattgttgc
    51301 ttgatccatt tctgatccag aaataagcac ctgctggaat agcgatacat aagaccagta
    51361 aaatcaaaaa tagcgaaaga aataatgtat ttcctttctt ttgttgtctc tgagaacttc
    51421 ttgatgaaac ttcatcatct gtatcgtaga ttggttgttc ccatggttct tgtgtttcgt
    51481 tattttctgt agattgatgt ctatcttttc tgctcactgt tttacctcct aaactctatc
    51541 gtgtgtatta taccatagga aaaagtgtct tgtcctaaaa aatcaatgtt ccatgttttt
    51601 tttaaataaa tttagtaaaa tcgttcccca atgaagttgt tcagtcttag aaaaaagctt
    51661 cctctttggc agaagttctg cttttgctcc atcaatatca caacaattct gcggcttttc
    51721 ggagagtttt tcgccaaaat aagctaagat gaattttctg cggcagtttt cttcattaat
    51781 atagcgaagc atctgctgca ggcggaattc tttttctttt tcctgacgtt tgagccgttc
    51841 cacccaattt tctggttttt cagactgctg gatcagctcc atccattttt tttgcagctc
    51901 atcaaaggga gcttgctctg tggcatattc caaataaagc tcaaaacttt gtcgctgttc
    51961 tctagaaagt tgattaaaga aataatggat acgttcatcc ccactttgat aaaaaagaat
    52021 tgctgcactt tcttcctgat ctctgcctgc tcgaccgatt tcttgtacat agttttctag
    52081 actgtctggc aaatcataat ggaccacata acggatatca gatttatcga tccccatacc
    52141 aaatgcattc gtagcaatga gaaactgcag ctgatttttt acaaactgct gttgaagttg
    52201 tcttctttga gctgcatcaa gaccaccgtg atagtatcca actgtaaaac gttccctgaa
    52261 taaatgatac aatcgttcga cttctttttt tgttgcacaa taaatgattg ccgctccttg
    52321 tgctctttcc atgaagtgtt caagttcttg ttctttttgc tgcgttttcc gaacaaaaag
    52381 cgagatattt tgccgattga ctgattgtct gatctctttt ggtggtcgat caaatagtac
    52441 atgacgtata tctcttgcta ctaaatctgt tgctgtcgcc gttagtgcca gtgtgaccgg
    52501 gtttcctaaa agctttcgga tttttcctaa ctgttgatat tctggacgga aatcaactcc
    52561 ccattgagac acgcaatgag cctcatctac tacatataac gcaatatttt gttttttcag
    52621 atgctctaaa agagaaggat tagtcaacat ttcgggactc aaaaacaaaa atttatattg
    52681 cgatagatga cggaggacgt atcttctttc agattgcgaa agtaaactat tgaaagcagc
    52741 tactcgtttc tcccctcgtt tttgtaattg cgtcacttga tcttccatta gagaaagcag
    52801 cggtgacacg atgatgacca gaccttccat taaatacccc gtcaattgat agcaaaggct
    52861 ttttcccgtt cctgtaggca aaatagcgag agtatcttct ccatcaagca acgcctgaat
    52921 aatttcttct tggccgggac gaaatgaatc aaatccaaaa tattttttta attcatttat
    52981 taacattctg tatcgcttcc tttctcaata gatagatttg atacaaacga aattctccat
    53041 aatctaattg tccatctaca ttccattcgc taaagcgcca ctctcgcacg gaatccttat
    53101 ttggttccaa acgttcgatt gtttctgcag agagaatcca ctcgtatgga aaatcatcaa
    53161 aaaacaaggc ccactcaata agatgatctg ttactgttcc tctttttaaa tgacggattg
    53221 ccatgatctg gtcaattgtc tgaccattca taaacatctg cttggtgatc atcatgcttt
    53281 gattgaaatt ttgctgtaat aatgaatcga tcaaagcgtg ccaaagcgaa tctggccgtt
    53341 tttcgatttg tgctaacaaa aggtggatac atctgctttg ttgcagataa accgctgact
    53401 catcattgtt ttttgccagc tgataaggaa gcagccctgt tcgctcattt ccagataatt
    53461 ggttagctaa aaaatctgct gcttcttctg gtatctccga aaacagctgc accaaactct
    53521 catacgcagt atcaatcaaa atttcacgtg gcaattggct ttcactaagc cattttttta
    53581 gttggaaagt ataaaaagga cttgtctctg ctggaagata gtcttgaata cccgttgcca
    53641 gattagaaat tacttgaaca gcaaatttaa tgagccgcca actagtttcc cctgttctgc
    53701 catagcggtc aaaacgcaaa ccagtatgtt ctatttgatg ttcggacaac atatcagccc
    53761 ctgcaggcgt tagctttgct tcattttcat ttatttcgat ccaaccttgc tgcgcaattt
    53821 tttgcaaaat ttgataaaac ttatcctgct tcaattctgg aaaactgtta tggataaaca
    53881 aaagttcgtg ggtaaatcca taaataagta ctgaagtcgt tcgttttccc acaaggagtt
    53941 ggtaaagaga actaatccgc agcttgtgtc ctgatgaaaa tagcgataaa ataaaaacaa
    54001 tcatattatt ctccttaagt taggatggtt agaaatgtca ctactatgta aacttgtacc
    54061 tgaacgctgt attgcatgcg gtctttgtca aatcgaagcg ccagatattt tcgattatga
    54121 tgaagaagga atcgttttat ttgcccaaga accagaggct catcaacagt tcgtgatgcc
    54181 ggaagaagaa acatatgtaa aaaaagcgta tcaaaaatgt ccagttcgag cgattttatt
    54241 agaaaataaa atataaatat aaacgaaact ttaaaaacgt acactaagtt ctctaaactt
    54301 gaaaataagc cgggatctgt ttctcttgtt ccgtgaaatc ccgacttatt ttgaaagatt
    54361 catttgctat ctatagttaa ttgactagtt ttttcacgga ccctatagga cccatttata
    54421 ttatttcaac tttttcagcc agttttccaa tgtattgacg atcacagttt gctggtctgc
    54481 gttagaaatt tctgcctctc catctccttt ttgctcacca tagctgccaa aacctccatg
    54541 atttccgcca ttgattgata catactctgt tctttccggt aaatactgct tagcttcttg
    54601 ataagcttcc tgattcaata ctttatcttt tgtcgccgtg atcgataaaa caggaacagc
    54661 tgattctgtc aatgttcctt ttttgtcggg ataacttgcc atgaagaata ccccttttaa
    54721 atgtttgtct gaatgattct tattgacata acgacttgcc attacgccac ctaatgaatg
    54781 gccaccgatc acataatcgg aatttggaaa atcttctttg accttatccg ctttgtttcc
    54841 gcccatcact gctagatcaa gtggcatttt gacaatagcg actgaaaagc cgttttttgc
    54901 taaatcttgc gcccaaatac tataactttc tggctctacg agagcaccag gataaaaaat
    54961 aagtaatgga gactttccat gattcttttt ctctccttca aaaaataagt aatcttcttt
    55021 gttttttcct tcttttacca acacttgcgc ttctgacgat ggttcgtacg tattttgctg
    55081 tatatagtaa taaccggctc caccgagtaa tcctaaaatg atgactaagc caattagtat
    55141 tcgtctaaat attttcactt tctcccacct tttctatctg agtgacccct ttttcttaat
    55201 caagtatgat tataccttaa aaaaataaga gagaaatgca aactttccag catcaaataa
    55261 aaaaagacgg aatatccatt tgaatatccg ccctttttgt atgtttattt ttatttagca
    55321 cataaacgat acacagcttc tgcataaatt tccattgctt tatacatact acttaattcc
    55381 catcgttcat tcgcctggtg cataaagtca ggagtatcag ggaacatcgc accgaatgcc
    55441 acacactggt tcatcgtacg agcaaaagta gcgccgcctg atacaaacgg ttcagtcatg
    55501 tctccagtaa tatcgcgata tgtccccaat agatttttca ctaactcact gtctagtggc
    55561 acgtataatg aatcaaggaa gtcaaattct tcataagtca acccatattt ttctgctgtt
    55621 ttagttaatt tagctactaa atcatctttc ttgaatgtaa cagggattct catatctaca
    55681 ccgatttttg tttgttctgg tgtgatttcc aaacttgcaa agttcatcgt tagctcgccg
    55741 gattgttcat cttctgtttt gcccacgaca gcttcaccag ttgcattttc ttgaactaat
    55801 tttcctaaga aattgattgg gccaaaatca aatacttctg aaagagcaat tgccagacga
    55861 gaaatagcat tcactccttg tgctgcatct ttcgcgtgga tacttttacc aagaacgacg
    55921 atgccttcac cttgttcttc gaaatcgaat tcgtgttttt tcaatgcttc ttttacttca
    55981 gaaagctttt ctccagagta aggagctgca tcaggaacaa cattcaacgc tcctccagct
    56041 tttacagaaa actcgttcgt tcctggtcct gtcaagtaag cttgcaataa gcctttttct
    56101 gcataaatta aaggaaattc agcatctggc gcaaatcctt gcgtaattcc ttcttctttt
    56161 tcgttatatt tttctaaaca acgccataaa ttttcttcat ctgtaccaaa gataaaacga
    56221 atacgtgtct tgaattccac gccagcatca atcaaagctt tgacggcata cattgccgca
    56281 atagaaggac ctttgtcatc ttgggatccg cgaccaacta accagccgtc ttttatagtt
    56341 ggatcgaatg gttttgtttc ccaattattt tcgtcccctg ctggaacaac gtccatatga
    56401 cacaagatac cgaataattc atctccagag ccaatctctg aataaccgta atagccttct
    56461 ggatctttaa atgttctaaa tcctagcttt tcactgattt ccaatacttt atccaaagct
    56521 tcaatcactt ttttaccgaa gggatgtcct tgtcctgaat ctgcttcatc tagtacggaa
    56581 ggaattcgga tcaattcgct caatgattct aatgcttgtt catgatgttg ttctgtcaca
    56641 aattttttca tgatattatc ctcctaatat gttaaaaagg gtcatgtcaa attcctccga
    56701 cacaacctct tcttctcaaa taatgacgtt ttaagcctgc tccatcagca agcagaatgt
    56761 tcttaacaaa tgatgaaaga aaaaacactc actgaatgga gctattgact tatccacgtg
    56821 cttatcgata gccgtttatc ggtttcttca ttcttatgat agaccttgta cctgtatttg
    56881 tctaggttta gaacattgct gctacaccta ggatcacaca gatggctaca aataatatcg
    56941 caattaattt accggtaaat ttccaccaca ctgaaacatc gaaacgagca attgctacag
    57001 cacccattac gatagcagat gttggtgtaa ttaagtttac taaaccagat gcagattgat
    57061 aagcagtgat tactaaatcc ttaccgacgc cggcaaattc acccataggt cccatgattc
    57121 ccattgttgc agcagctaag ccggaagttg aaggaatcaa gaatgacata gggatataga
    57181 aaataaatgt aataatgatg aatacacttg aagataatga acttaatcct tgttcgcccc
    57241 aatgtaagat ggtgtctgtg attaatccat tgttcataac cacttggata ccacgagcaa
    57301 cagctactac gattgctaca ccgatcaaat cagaagcacc agctaaaaat tcagagacaa
    57361 attcagactc tttcatgcca taagtgattg ctactacgat ggacattact aagaataaca
    57421 tagtaatttc tgggaagtac cagtcaccta atggaagcat gcttttacct agaaccgtac
    57481 ctagtaccgg aaggtttgtt aaccagtttg tcaatgattc aaaaatcgtg aaattcgcat
    57541 tcaaggttgt ccaaggaatc aaactgatga tcatgatacc aaaagtaata aagaacaata
    57601 cattaacatg tttttgtttt ttcgtgatta cttcttgacg accgattgct tcaatatcga
    57661 aatgcttcat atcttcttta cgttggttgt atactaatga ttttgttgga tctttttcga
    57721 tctttgatgc ataacgatac acatatacaa tagaaatagc taacaaaata acgaacatca
    57781 agaatcgaag accaatacct tcacccatcg aaatcccaac agcttgtgaa gctacgcctg
    57841 ttgcaaaagg atttaccgtt gaagctagac agcctacttg agaccctact aatacgattg
    57901 ctacagctac tactgtatca aagcctacag ccatcatgac tgggatcagt aaagcataga
    57961 atggaatcgt ttcttctgcc attccgtaag tagagccgcc taatgcaaat aacagcatca
    58021 atactggaat aagcattttt tcttttcctt tgaatttctt cacaactgaa gcaatcccgg
    58081 catctagtgc ccctgttttg ttgataatac ctaaaaatcc accaacaacc aaaatgaaga
    58141 aagagattgg gattgcagca ggtgtttctt tagttagctc ccccgctggt acacctagca
    58201 tcccattgat tggcgccatg aaaatatccc acagcccttg aggattttgt tgtactcgtt
    58261 catatgatcc agcgacgatg ttgcctgcat cgtctacact atagtgccca gctggtataa
    58321 accaagtaag tacagcgatt actgcaataa taataaataa gaccgtatac gctgaaggca
    58381 tttgaaattt tttctttttt gtttgttcca tcttcttctt cctttctttt ccgttagaga
    58441 caacaatttt ggctctaatg ttattttatc aaaaagagta tcagaacaaa aataataaca
    58501 cgcattttgt aaaacttttg ttcaaaaagt tcgtttttcc ctattccctc ctcttttttc
    58561 aggtttttaa agctttcttc tttttcattg tagcgtttcc ttttttttat aaacaaacat
    58621 tttcgtcttt ta
//
LOCUS       DASJSZ010000006        57037 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.006, whole genome shotgun sequence.
ACCESSION   DASJSZ010000006 DASJSZ010000000
VERSION     DASJSZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.006"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            44..1561
                     /locus_tag="U4B03_000343"
     CDS             44..1561
                     /locus_tag="U4B03_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN2067223.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1533..2822
                     /locus_tag="U4B03_000344"
     CDS             1533..2822
                     /locus_tag="U4B03_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2067224.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            2997..3893
                     /locus_tag="U4B03_000345"
     CDS             2997..3893
                     /locus_tag="U4B03_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2067225.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3907..4770
                     /locus_tag="U4B03_000346"
     CDS             3907..4770
                     /locus_tag="U4B03_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2067226.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4776..5339
                     /locus_tag="U4B03_000347"
     CDS             4776..5339
                     /locus_tag="U4B03_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN2067227.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5361..6872
                     /locus_tag="U4B03_000348"
     CDS             5361..6872
                     /locus_tag="U4B03_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN2067228.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6884..7843
                     /locus_tag="U4B03_000349"
     CDS             6884..7843
                     /locus_tag="U4B03_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN2067229.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            7929..9008
                     /locus_tag="U4B03_000350"
     CDS             7929..9008
                     /locus_tag="U4B03_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2067230.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(9260..10660)
                     /locus_tag="U4B03_000351"
     CDS             complement(9260..10660)
                     /locus_tag="U4B03_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN2067231.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            10878..12539
                     /locus_tag="U4B03_000352"
     CDS             10878..12539
                     /locus_tag="U4B03_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEN2067232.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12496..13194
                     /locus_tag="U4B03_000353"
     CDS             12496..13194
                     /locus_tag="U4B03_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN2067233.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            13210..14634
                     /gene="araA"
                     /locus_tag="U4B03_000354"
     CDS             13210..14634
                     /gene="araA"
                     /locus_tag="U4B03_000354"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEN2067234.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            14941..16101
                     /locus_tag="U4B03_000355"
     CDS             14941..16101
                     /locus_tag="U4B03_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEN2067235.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            16219..18078
                     /locus_tag="U4B03_000356"
     CDS             16219..18078
                     /locus_tag="U4B03_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2067236.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            18188..18622
                     /locus_tag="U4B03_000357"
     CDS             18188..18622
                     /locus_tag="U4B03_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067237.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(18881..19042)
                     /locus_tag="U4B03_000358"
     CDS             complement(18881..19042)
                     /locus_tag="U4B03_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067238.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(19061..20083)
                     /locus_tag="U4B03_000359"
     CDS             complement(19061..20083)
                     /locus_tag="U4B03_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEN2067239.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            20211..20564
                     /locus_tag="U4B03_000360"
     CDS             20211..20564
                     /locus_tag="U4B03_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN2067240.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20576..21742
                     /locus_tag="U4B03_000361"
     CDS             20576..21742
                     /locus_tag="U4B03_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2067241.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(21834..22454)
                     /locus_tag="U4B03_000362"
     CDS             complement(21834..22454)
                     /locus_tag="U4B03_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEN2067242.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(22646..22942)
                     /locus_tag="U4B03_000363"
     CDS             complement(22646..22942)
                     /locus_tag="U4B03_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2067243.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            23146..23796
                     /locus_tag="U4B03_000364"
     CDS             23146..23796
                     /locus_tag="U4B03_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN2067244.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            23812..25539
                     /locus_tag="U4B03_000365"
     CDS             23812..25539
                     /locus_tag="U4B03_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2067245.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            25536..27428
                     /locus_tag="U4B03_000366"
     CDS             25536..27428
                     /locus_tag="U4B03_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2067246.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            28132..29001
                     /locus_tag="U4B03_000367"
     CDS             28132..29001
                     /locus_tag="U4B03_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEN2067247.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            29210..30235
                     /locus_tag="U4B03_000368"
     CDS             29210..30235
                     /locus_tag="U4B03_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN2067248.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            30274..31224
                     /gene="manA"
                     /locus_tag="U4B03_000369"
     CDS             30274..31224
                     /gene="manA"
                     /locus_tag="U4B03_000369"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEN2067249.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            31318..33021
                     /locus_tag="U4B03_000370"
     CDS             31318..33021
                     /locus_tag="U4B03_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2067250.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            33023..35056
                     /locus_tag="U4B03_000371"
     CDS             33023..35056
                     /locus_tag="U4B03_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2067251.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            35067..35507
                     /locus_tag="U4B03_000372"
     CDS             35067..35507
                     /locus_tag="U4B03_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2067252.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(35707..36834)
                     /locus_tag="U4B03_000373"
     CDS             complement(35707..36834)
                     /locus_tag="U4B03_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2067253.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(36837..37514)
                     /locus_tag="U4B03_000374"
     CDS             complement(36837..37514)
                     /locus_tag="U4B03_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2067254.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            37778..39544
                     /locus_tag="U4B03_000375"
     CDS             37778..39544
                     /locus_tag="U4B03_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEN2067255.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            39559..39996
                     /locus_tag="U4B03_000376"
     CDS             39559..39996
                     /locus_tag="U4B03_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2067256.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            40009..41160
                     /locus_tag="U4B03_000377"
     CDS             40009..41160
                     /locus_tag="U4B03_000377"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEN2067257.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(41222..41920)
                     /locus_tag="U4B03_000378"
     CDS             complement(41222..41920)
                     /locus_tag="U4B03_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEN2067258.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(41913..43265)
                     /locus_tag="U4B03_000379"
     CDS             complement(41913..43265)
                     /locus_tag="U4B03_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEN2067259.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(43488..44888)
                     /locus_tag="U4B03_000380"
     CDS             complement(43488..44888)
                     /locus_tag="U4B03_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEN2067260.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            45049..45786
                     /locus_tag="U4B03_000381"
     CDS             45049..45786
                     /locus_tag="U4B03_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEN2067261.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            45911..46390
                     /locus_tag="U4B03_000382"
     CDS             45911..46390
                     /locus_tag="U4B03_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEN2067262.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            46387..47154
                     /locus_tag="U4B03_000383"
     CDS             46387..47154
                     /locus_tag="U4B03_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067263.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            47384..47953
                     /gene="lepB"
                     /locus_tag="U4B03_000384"
     CDS             47384..47953
                     /gene="lepB"
                     /locus_tag="U4B03_000384"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2067264.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(48057..48659)
                     /locus_tag="U4B03_000385"
     CDS             complement(48057..48659)
                     /locus_tag="U4B03_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2067265.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            48765..49234
                     /locus_tag="U4B03_000386"
                     /pseudo
     CDS             48765..49234
                     /locus_tag="U4B03_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            49340..49924
                     /locus_tag="U4B03_000387"
     CDS             49340..49924
                     /locus_tag="U4B03_000387"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN2067266.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            49927..51000
                     /gene="prfA"
                     /locus_tag="U4B03_000388"
     CDS             49927..51000
                     /gene="prfA"
                     /locus_tag="U4B03_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEN2067267.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            50993..51832
                     /gene="prmC"
                     /locus_tag="U4B03_000389"
     CDS             50993..51832
                     /gene="prmC"
                     /locus_tag="U4B03_000389"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN2067268.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            51825..52004
                     /locus_tag="U4B03_000390"
     CDS             51825..52004
                     /locus_tag="U4B03_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067269.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            52026..53051
                     /locus_tag="U4B03_000391"
     CDS             52026..53051
                     /locus_tag="U4B03_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN2067270.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            53242..54486
                     /locus_tag="U4B03_000392"
     CDS             53242..54486
                     /locus_tag="U4B03_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEN2067271.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            54639..55586
                     /locus_tag="U4B03_000393"
     CDS             54639..55586
                     /locus_tag="U4B03_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2067272.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            55720..56349
                     /gene="upp"
                     /locus_tag="U4B03_000394"
     CDS             55720..56349
                     /gene="upp"
                     /locus_tag="U4B03_000394"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEN2067273.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(56488..56730)
                     /locus_tag="U4B03_000395"
     CDS             complement(56488..56730)
                     /locus_tag="U4B03_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067274.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            56851..56923
                     /locus_tag="U4B03_000396"
     tRNA            56851..56923
                     /locus_tag="U4B03_000396"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56884..56886,aa:Thr,seq:cgt)
BASE COUNT        18931 a         9991 c        11829 g        16286 t
ORIGIN      
        1 caacgaggtg tattattcat gtataaatta aaggaggtca gttatggaag ccacaatgtt
       61 gattagtagt gtcaagcgtg taggcaaagt tgatccgcgg ttgtacagtt catttattga
      121 acatatggga cgagcagtat atgaagggat ttatcagcca gatcatccat cagctaataa
      181 aagtggattt cgccaagacg tcattcactt ggtaaaagaa ttgaacattc ccatgattag
      241 atatcctggt ggaaattttg tgtcaggatt tcgttgggaa gacagtattg ggcccatagc
      301 aaatagacct agaaggttgg atttagcttg gcggacaatt gaaacgaatg aagttgggat
      361 tcatgaattt tataactggt gtcagcatgt gggggccgaa atcaatatgg ctgtcaatct
      421 gggtactcgt ggagtagatg cagctcgtaa tttagtcgaa tattgtaatt ttcctaaagg
      481 aacttattgg agtgatttaa ggcgttcgaa tggtcaagag agcccttttg gaattaagac
      541 atggtgttta gggaatgaaa tggacggtcc ttggcagatt ggacataaaa cagcggaaga
      601 atatggacgg ttagcagagg aaacggctaa ggcgatgaaa ctcgtagatg attcaattga
      661 ggtggtttta tgcggaagtt cgaatcggca aatgcctaca tttggtgatt gggagttaac
      721 agttcttgat caagcgtacg atcaaataga ttatttgtct ctgcatcaat attatggcaa
      781 ccaaaaaaat gatctttctc attatcttgc acgttcctta gacatggatc ggttcatttc
      841 tgaagtgaca gcgatgtgcg atacagtcaa ggcgaagaaa catagtaaga agcagatcaa
      901 cctgtccttt gatgaatgga atatttggta tcattcagcg gaacaagatg agcaagcagc
      961 gccttggcag attgctcctc ctttactaga agataactat aattttgaag atgcattact
     1021 attaggctgt ctattaatca ctttattgaa acatgcagat agagtaaaga ttgcttgtct
     1081 tgctcaattg gtaaatgtta ttgcaccaat caggacagaa acaaacggag aagcttggag
     1141 acagacgatc tattatccat ttatgcaagc tgctgtgtat ggacaaggag aggtgttaag
     1201 cccacaaatt cgttcagcaa actatgcgac ggaagatttt gaagaagtgc cgtatttaga
     1261 aagtatagcc gtaataaatg acaaagaact tgtcatattt gcagttaacc gtggagaaga
     1321 agaaatgaat ctaactgttc acttgtctga attatcatta gagggagtga ttgatttttc
     1381 tgaaatgagt ggatttgata caaaagcaac taatcttcct aaaagtgaac aagtaaaacc
     1441 aagtcattca acaaatgtaa aagctgaaga tgggaaaatc aattgtttat tgaaaccgct
     1501 atcttggaat gtggtcagat gtcgtttatc agatgaagga ggaacaaaaa atgaaaggta
     1561 aaaaatggtt aattggctct ttgttggtag ctgcaacagg tattttagga gcttgcggtg
     1621 gcggaagttc agcagaatct gattctgata aagaagtgac tttgactttt tggaatgggt
     1681 ttactgcatc tgatggtgaa atattgcaag aaatcgttaa tgaatttaat aaaacaaatg
     1741 ataaaaacat caaaattgaa atggatatca tgacttgggc caatttgaat gaaaaacttc
     1801 ctacagcgat ttcttctaaa aaagcacctg actttattgc attaaattat ccagattttg
     1861 cacagtatgt agaaaacgga gcggtacaac cattagacga tttctgggaa tatgatggag
     1921 tagataaatc tgattttaaa gaaacggcta ttgaattagg tgaagtagaa ggtaaacagt
     1981 attttgtccc catgcaggta caaggcatgt atatgtattg gaataatgat ttgtttaaac
     2041 aagctggatt agatactgaa caaccaccta ctacttggga agaattgaca gaaatggcac
     2101 caaaattaac tgatagtagt aaaaatgtat caggttttgt cttcaataag gatggaactg
     2161 caccgttata taactggatg ttagcaaatg gcggtaaatt agtgaacgat gattatacta
     2221 agtctgaatt tgcgtctcct gagaacctta aaacgttaaa agcgatccaa aaaatgatcc
     2281 atgtagacaa gactggtcca gaatcgattt ctggcgccga aatggacaat ttaatgaatg
     2341 ctggtcaact agccattgaa atcaatgggc catggttaaa taacggattg aaagcaaacg
     2401 agattgatta taatgtgact accttaccac aagggagtga ttcagacaaa aaaacagcta
     2461 ttttagatgg cgtaggatat gccattccgg caagtacaga taataataaa aaagaagctg
     2521 tctatgagtt tttgaaatat tggaatacaa ctgaaattgg taaaaaatgg agtatcgaaa
     2581 atggttttcc tgcctacttg gattcagtag caaaagatga agaagtaaaa aatgatccta
     2641 ttgtatctga attatcgaaa caaatggact atgcagagcc atatttacca ggttttaccc
     2701 aaatctctgc gatcaataac gatatcatca atccactgat tgaaaaatta ttagcaggag
     2761 aagatcctga gacactcatg aacgatgcag atgatgccat caatcaatta ctgcaaaaat
     2821 agtacagaac tttatacagg atagactgtt ggcaagatac agatatctga atgatcgacc
     2881 tagtatttgc agataggtgt tattgcaaaa atcacagcga ttgttcggat atcttattca
     2941 gattcgttta aaccaagatt gtatatttta tcaaaatgaa agatgaggtg gactatatga
     3001 acggattcac caaatattgg aatcgtcctg atcgtgctgc gtatttgttt ttaactccat
     3061 cttttttagt tttattatta tttactgtca taccgctttt aggaacattt gctattagtt
     3121 ttacagattt gaatattttc tttacatcaa gaaattttgt aagttttgaa aatttcacac
     3181 gtatatttag tgatgaacga gcagttaatt cgttgattca ttctttatat ttcaccgtac
     3241 tcgaaacgcc aacacaaatt atcgttggtt tattagcagc agtagcatta tctaaaaata
     3301 ccagattcaa tcgtttttgt cgttcgacat tttacattcc agttatttgt tcattgacgt
     3361 caattgcgat tattttttct atgttgcttg atccgaatgt aggggcgatt ccttacttgt
     3421 tttcacaagt cggcttgccg atccctcaat tttttcgcga tccaaccctt gctatgccga
     3481 cggtagccat catgactgtt tggaagaatt ttggtgttac tatgacgatt ctcttgactg
     3541 ctattcaggg aatctctccc tctttgtatg aatctgctca aatggatggt gcaacaaatc
     3601 gacagcagtt ttttaacatt acgctacctc agatcgttcc ctccttaggt ttttgtattt
     3661 taacgaatct gattggttcg atgcaagtgt ttgaccaagt atatgttgca accgctggtg
     3721 gcccacaatt taagacagaa acagctgttc aatatattta cagtcgagga tttacagcac
     3781 cttatgaatt gggctatgca tcagcactgt cttcggtttt gtttatcatt gttgcaacta
     3841 tagctatctc cactaatgtg tacatgtcac ggaaagaaaa acaaatgaaa taggagggaa
     3901 acctgtatgg caccagaaag ctcgattaca gttactgtga atagtcgaaa agcagttagt
     3961 ggtaaaaaaa tcattcgaac gattagtatg ctagttatat tgttggttat tgcgtttcct
     4021 tttttgtggc tgattatttc ttcttttaaa cacgaaaaag acattatctc atttccgcct
     4081 agaatatttg cagatagcta taccctagat aactatatta aggtttggac aacgatccct
     4141 ttattagatt acatcaaaaa tacagtgatt tttgctggag ggacagtgat cacttctgta
     4201 ttctttgatt ctcttgcggg gtatgcattt gctcgtatgc gtttcaaagg aaaatcagtt
     4261 ttgttctatt tcgttttgct gacaatgatg atcccattcc aagtcttcat gattcctcta
     4321 tttattgaag tcaatttgct tggcatgctt gatacttatg caggtttgat cattcctcgt
     4381 atgacaactg catttggtat cttcatgatg cgttcctttt ttattacttt accagattct
     4441 ttggaagagg cagcacgcat tgatggatta agtgagttta atattttttt gaaaatcatg
     4501 ttgccattga gcaaacctac tcttttatca ttagggattt tcacgctgat gaatagttgg
     4561 aatgacttgc tttatccatt gatattgaca agtagttcaa aaatgagaac tttaccagca
     4621 gggttagctt tatttacagg gcagaacatt tctttttatg gacctgtaat ggctggaacg
     4681 gttatttcaa tgctgccatt actagtggtt tatatttttg cacagaaata ttttgttcaa
     4741 ggaacagcta tgtctggaat gaaagagtga ggaagatgaa aatagttgag actaaacttt
     4801 atggacagtt gattgtggtt tctgattaca tcttattagg tattctctgg gtaatcgtgt
     4861 gtttgcctgt tattactatc gtgcctgcta catgtgctgt tttttatgta atggcgcaat
     4921 ggaaaggtga gggatcagga aatatcattt attttttctt tcaaggaata aagcaacatt
     4981 ttggtaagaa cttgttcctt agtctgttaa cgatcattat ttattttctt gttaatcaat
     5041 tattagaaga acagacaatc gtactttcaa ttagtggata tgtagtttta cttatttatt
     5101 ccatgtttct tttatcctgg atatatgtga tttctgaaaa caaagatatc acatttttac
     5161 aaatttttga acaaagtgca tggaaagtat tttttgattt tttgagaaat attctttgtt
     5221 gcgtattgtt attaggattt gtactgctca tatttctttt ccctcctttt atttttatct
     5281 ttgctggtgg cgtatggaaa cttgtatcta ttatcttgac tataggaaat gaaaggtagg
     5341 gaaagctgtt ggacgaagaa gtgaaagtaa aaatcagaga ggattttacg gtaggagaga
     5401 tcagttctcg gctgtttggc tcatttgttg agcatatggg aagtgtcgtc tatacaggga
     5461 tttatgaacc gggccatgaa acagctgatg gtgacgggtt tagacaagat gtcctctctt
     5521 atgtaaagga attagaatta ggtgtcattc gctatcctgg aggaaacttt acatctggtt
     5581 atgattgggt ggatactgta ggtaggaaag aacaacgacc aaaaaaaatt gatttagcat
     5641 ggcgaggtat agaaccaaat tcttttggac tccacgaatt ttttaaatgg atcagtcatg
     5701 tacaagcgaa accgatattc acggtgaatt taggaacaaa aggaattgat gaagcaaaaa
     5761 atttagtaga atattgtaat tttccaaaag gaaccttctg gagtgattta agaataaaaa
     5821 atgggcaaaa aaagcctttt ggcgttaaat tatggtgttt agggaatgag ctagacggtc
     5881 catggcaagt gggggctaag agtgcttatg aatatggtag gttagcaaat gaagcctcaa
     5941 aagtcatgaa gttagtggat gattcaattg aaacaatctt agttggaagc tctacgccac
     6001 gtttaccatc gtaccctgaa tgggatcgta tcgcattaga agaagcttat gagaatgtag
     6061 attatttagc tttgcataac tatattgata agtatgatga agaagacttg actaaacctc
     6121 ctaaaaatga gcgtgatgat acagcgacat accttgcaaa agcttatcga ttcgatcgtc
     6181 aaattgaaga aatcgttgct acttgtgatt atgtaaaagg atatttgaga agtgagaaaa
     6241 caatgtatct agcgtttgat gaatggaatg tacatgccca accggaaaaa agacataggg
     6301 attttgaagt cggtagtccc atcgattggt gttactttac tatggaagat acattattat
     6361 ttggttcatt agggctggct attttacgac acgcagatcg cgtaaagatt agttgtcaat
     6421 cattgttagt aaacacaata cctttaatac tgacagatgc taaaggacaa gcatggagga
     6481 atccaactta ttatgtgatg caacacttat cgaaatatgc taaaggtcaa gtattgaaac
     6541 aacaaatcat ttgccctgtt tatgattgtg aaaagataaa aaatgttcct tatgtggaca
     6601 gtgtgatcgt agataacggc aatcagctga ctattttctt gatcaatcgc acaaatgaac
     6661 aacaaaagat aacgattgaa catgatttca atttgctaga tcaaggagag cactacacat
     6721 taaccagtag cgatctgttg gacaaaaata cacgtgaaga accagaaaaa ataaaacctg
     6781 aaataaacta tttttcaata gaaaaacatg acgaattaaa tgtagggata gaaaaatact
     6841 cttggaatgt aattagaata aagaaaatgt gaggaaattt gttatgatga agacacttta
     6901 taataatcct atcgttcggc aacgggcaga tccttgggta tataaacata cagatggtta
     6961 ttattatttt acaggatcgg ttccaggcta ccaagtgatt gaattaagaa gggcaaagtc
     7021 actgaatgaa ttagaacatg ctgcaagcct gattgtatgg caggctcatg aagaaggacc
     7081 gatgagtgaa ttgatctggg cacctgaagt ccattatatc aatggaaaat ggtatattta
     7141 ctttgctgca agcaatcata aaactatacg tgatgaatct catcatcacc gcatgttcgt
     7201 acttgaaaat cagcatatag atccaatgaa tacagattgg atagaaaagg gacaaataaa
     7261 aacgaaaagg gaaactttca gtttagacgg aacaactttc tcttctcaag gaaagctata
     7321 ttatgtgtgg gcccaacaag atcctgaaat tactgggaat tctaatcttt atctatccga
     7381 aatggataat ccttggacgt taaaggggaa gcagatcctc ctttctatac ctgagtatga
     7441 atgggaaaaa cggggttttt cagtaaatga aggtccagca gtccttactc gcaatgggaa
     7501 aatattcatt acgtattctg gtagtgcaac agatgaaaac tatgccatgg gcctgctgtg
     7561 ggctgatgag cacaagaatc ttttattaaa agaatcatgg cataaattat cggagccagt
     7621 attcgtttct tctaaaaaaa ataagcaatt tggtcctggt cacaactcgt ttacggtttc
     7681 agaagacggg tgctttgata ttttgatcta tcatgctcga ccagagaaga accaaaaagg
     7741 ggatccgtta gacaatccta atcggcacgc gaacgcgcaa tgctttacgt ggaatgagca
     7801 aggatttcca gaatttggtg aacctgttcc gtatgcctta tgagcacaag aaaccttgat
     7861 ttttgcttga ataaaccttg cagactcgtt atgatatatg tacgtacaaa taaaaagggg
     7921 caaggcaaat ggcaaaatat caagatattg cagaagagat aaaacaaaag atccttcgtg
     7981 aggaatacga agtaaataca accattccct cagaattcaa gctgcaagaa atatatgacg
     8041 tcagcagaca tacgattcgg caagcaatcg ctgtgttggt aaatgagggc tatctgcgta
     8101 aagaaaaagg gtcgggcaca tatgtagatg acagctataa gaaagaaaaa acaccattga
     8161 acagaacaaa aacgatcggt gtcatcacca catatctctc tgattatatt tttccgtcga
     8221 ttatccgagg aatcgaaaat gaattacgag aatctgggta ttcattgctt ttagccagta
     8281 cgaataacga tcacgaattg gagaaagagt gtttgaagaa aatgattgct tatggggtag
     8341 atggtttgat cgttgagcct acgaaaagta atcagtataa cccaaactta gcactttatg
     8401 ttgctttgag agaacaaggc attccgatgg tgatgatcaa tgctgcttat gaagagttaa
     8461 atgcctctta tatttgttta gatgatgtga aagtcggttt tatggcagca gaatatttga
     8521 taaaaaaagg acatcagcgt ctgttgttca tcacaaaaat cgacgatctt caaggaaaat
     8581 acaggatgaa aggttttatc agcgcgtgtg aacagtataa tgtgcgtttt ttgacggaag
     8641 atatcattac tttcaccacg gaaacacgag aaacaatcgc tgaaaaaaca gtacaacggt
     8701 tgaaagaaga acaagcttta gatggcatca tttgctataa cgatcaaata gctagtttaa
     8761 tgctagataa gttgacagaa aatggctatc atattccaga tgacctatcg atcgtaggaa
     8821 cagacgattc ttctttaagc catttaggga ataaaaaatt gacaacattg acgcatccaa
     8881 aagaaaagtt gggacaagat gccgcacggt ggatcgtcac aacgattgaa aaaggacagg
     8941 cacagccaaa tattctctat gaacctgtac tagttgaacg ggaaacagtg aaagaaaacg
     9001 aaaaataaga gacgggtttt taccgcctct tattgctcct acggttactc gtcgcaatgt
     9061 ttgtgagagc ttttgtcatc gctcaaggct gaacgtttct ttcaccacct ctccactaaa
     9121 cggtgttctc ctaccaactg gtcacaagtc acaatattca tcaaacatga gaagctgttt
     9181 tttgaaattg ttccttgtga ctggaaacta agcggttcct tcttatttct tgaagctgac
     9241 cacttctgtc acaacctctt taagaatgat ggatagaaat acgtttagga taagtttttt
     9301 tagatgcact ttgttcgatt tcctctaaag atttcccacg agtttctggt acgatctctt
     9361 gaataaataa aacccctaaa aagcagatga tcccaaaaat gccgaaaacg atttcttgag
     9421 acatagaagc agtcatcaca ggaaaaagca agccaactag gaaagaacca atccaattaa
     9481 aagaagaggc taggccagat gctcgcccgc ggacagctag tgggaatatt tcgcctacga
     9541 ttacccatgt cagtggagcc catgtgcaag aataaaatgc gacataaata cataaaaaca
     9601 agagaatcaa caacgaattt gtatgagcat ccagtacagt tcctaaaaca gctggtagga
     9661 tgaaagaaag tcccatcacg ctaccgccta ttttcaataa agttcgccga ttgaacttgt
     9721 ctgcaataac caaaaagagc aaagaaccag caactaaaat gattccttgg atgatcggcc
     9781 acatcaatgc atcgctcgct gcatgcccag tcgctttttc tacgatcagc ggaatatagt
     9841 aaaaaatagc attagctcct tgaaattgct ggaatactgc tacaccaata ccggcaatga
     9901 ctaaggaacg atatttttct aaaaacaaag tatgccaaga agttccggtt gtcttttctt
     9961 gtttgacatt cagctggatt tgttcgaatt cctgttcagc ttcttttggt gtccgaatat
    10021 agttcaatac tctttttgcc tcttccaagc ggccagattg cataagaaaa cgcggtgact
    10081 ctggtagagc taaaacaccg aaaaataaaa tcagcgcggg gacagcagcg agccctaaca
    10141 tcacgcgcca agccatcgtt tcaggcaacc cttttagcaa ataatctgca acataggaaa
    10201 ggagcatgcc agaagcaatc atcacttgat tgatccctga caatcttccc cttagtctag
    10261 caggagccat ttcagacata taagcaggaa ctagggcaga tgcagcgcca acagctaaac
    10321 ctagaagaat ccttgctgca atcagaaaat aagaaccatt gtgaggggca gatgcagaaa
    10381 ggacagaacc agccataaaa ataagtgcag aaagaagaat catttttcgt cgtccaagct
    10441 tatcagaaag aagaccagat aaagagccgc caaggatcgc acctaacatg accgaagacg
    10501 tgatccatcc tatcaaagaa gctttacctg ccaaacccca atcatgctgt aaaaaaggta
    10561 aggcgccggt cataacacca atatcatatc caaatagaat gccgccaaat gcgccaaaaa
    10621 aataaataaa cgatgaggaa actttttttt cttttttcat aagtattctc tccttctaga
    10681 atttcgagaa ggcggttgat cagattttgt aagcaatttc attttataac ttatgtacgt
    10741 acaaaacaag tgttttttat tgtattcttg taaataactg cttgacatgt acgtacaaaa
    10801 gtatacaata atcattgtaa gcgctactaa aaagacggtt tgatcaggtt tgacgagaag
    10861 gagggagtta aatcattatg aaggaagtaa atgcgcaaag tatgttagat gtcatagatg
    10921 gtcgtacatc tttagggatt gagtttggat caactaggat caaagctgta ttgattgatt
    10981 catcttttac tcctattgcc gctggtagtt atgagtggga aaacaaacta gaaaatggta
    11041 tctggaccta ttcattagaa gatatttgga aaggactgcg cgtcagttac cgaaaaatgg
    11101 cagcagaagt attcgaaatc tatggcgaga atcttacgac aatcgggtcg attgggttca
    11161 gtgcaatgat gcacggttat ctagcatttg atagcaagga acagttacta gtaccatttc
    11221 gtacttggcg aaatacaatc actgaagaag cagaacgaat attaacggaa aaatttcaat
    11281 acaatattcc gcagagatgg agtatcgctc atttgtatca atctattttg aataaagaag
    11341 aacatgtgaa agaaattaat tttttgacaa cgcttgcagg ttatgtccat tggcagctta
    11401 ctggtgagaa agtactaggc gtaggagatg catcaggcat gtttcctatc gatccttcaa
    11461 cgaaaaccta ccatcaagaa atgaaaaata tttttggtca gttaatcgaa aaagcgcacg
    11521 tagcgattga cttggatcac attttgccta aagttctagt agcaggagaa gaagctggca
    11581 agttaagcga atcaggggct tatctgatcg accctacggg aaatttgaga ccaggaatcc
    11641 cggtttgtcc gccagaaggg gatgcaggaa ccggtatggt cgctacgaac agtgtagcca
    11701 aagggacagg gaacatttcg gctggtactt ccgccttcgc tatgatcgta ttagaaaaag
    11761 aactagaaaa tgtccatccg gaaatcgaca tggtcactac accttcagga gaactagtag
    11821 gcatggttca tacgaataac tgctcttcgg atatcaatgc ttgggtcagt atttttgaag
    11881 aatttaccaa ttctttgggt atggagataa gcagagataa attattcacg gtattattca
    11941 acaaagcgct agaaggcgat acggattgcg ggggattact ttcttatggt tatttttccg
    12001 gtgaaaacat tacaggagta aatgaaggaa ggccattatt tgtacgtaca ccaggtagcc
    12061 gttttgattt agcgaatttc atgcggatcc atttagccag tgcttttggt gcaatgagaa
    12121 tcggaatgga tatattgaaa tctgagaatg tccaaattga tcgtttagtg gggcatgggg
    12181 ggatttttaa aacaccagaa gttggacagc ggattctagc atctgctatg gaggcaccag
    12241 ttacagtgat ggacacagcc ggagaaggtg gagcgtgggg aatcgcgtta ttagcagcct
    12301 atatgatgga tcgacatggg aagtctttag aaaacttctt ggcagaagac gtgtttggac
    12361 aagaccaagg aatcaccatc acaccttcag cagaagaaat agcaggctat cagatattta
    12421 tgagacgtta ccgtgagggg atcgacattg aaaaaacagc gattcatcaa ttgcaagaaa
    12481 aaggagtgat agaaaatgct ggaacaactg aaagaagaag tataccaagc aaatcttgac
    12541 ttgcctaaac acggactcgt caaatataca tggggaaatg tgagcgcttt tgatcctgaa
    12601 acacgatatt tcgttatcaa acctagcgga gtcagttatg aagagatgac agcagacgac
    12661 atggtcgttg tggacttgga caatcatatc attgaaggta aactcaatcc ctcttcggat
    12721 acgcctactc atgctgtttt atatcgttca tttcctcaaa tcggaggaat cgtccatact
    12781 cactcgacat gggcgacgat ctgggcacaa gcaggattgg atgttcctgc gatggggacg
    12841 acgcatgcag atacttttta tggatcggta ccttgtgctc gatttttgac acaacaggaa
    12901 atcgacagcg ggtacgaata tgaaacagga aaagttatca tagaaacatt caaagaacgc
    12961 aaaattgatc ccttagcaat tccaggggta cttttacacg gacacggtcc atttacttgg
    13021 gggaaagatg cgcaaagtgc ggtaatgaac gcagtcgtac tagatgaagt atgcaagatg
    13081 aatttattta ccagacagct caattcattt tcagaagagc tgcctcaacg gattttagat
    13141 aaacattatt tacgaaaaca cggaaaaaat gcgtattacg gacaaaacaa ataaaaggag
    13201 tggctaatta tgttgaatat tggagaaaaa gaattttggt ttgttgtagg atcacagcat
    13261 ttatatgggg aagaagcact gcgagaagta aagaaacatg cacaagaaat ggtcgatgaa
    13321 ctgaatgaaa atggccaatt gccttatcca atccgccttc aagaattagc agttactgct
    13381 gatacgatca caaaaatcat gaaagaggtc aattatcgag aagaagtagc aggtgtgatc
    13441 acttggatgc atactttctc accagcaaaa atgtggatcc gcgggacaaa gcttctacaa
    13501 aaaccattgc ttcatttagc tacgcaatat aacgaaagca ttccttggaa aacgatcgat
    13561 atggatttca tgaatttaaa tcaatccgct catggtgacc gcgaatatgg gttcatcaat
    13621 gctcgattaa ataaacaaaa taaaatcgtc gttggctact ggaaacgacc tgagatccaa
    13681 aaagagattg ctgattggat ggatgttgca gtagcctata atgaaagttt tggaataaaa
    13741 gtggctcgtt ttggtgacaa catgagaaac gtgggtgtca cagaaggaga caaagtggaa
    13801 gcacagattc aatttggttg gactgttgat tattttggta tcggagatct tgtacaagta
    13861 atcgatcgcg tatcagacga agaagtcgat caattatttg aagagtacaa ggaattgtat
    13921 accttcgact atggtgatta tgaagaaaag acatgggagg aacatgtaaa agtacaagct
    13981 cagcaggaaa tcggtattcg ccgtttctta gaagaaggag gctataacgc ttttacgact
    14041 aattttgaag acctgtatgg aatgaaacaa ctacctggac tagctgtaca acgtttgatg
    14101 gcagaaggat acggttttgc tggggaaggc gactggaaaa ctgcagcgat tgatcggttg
    14161 ctgaaaatca tggctcgagg gaaagataca ggattcatgg aagattatac ttacgagcta
    14221 gctagtggcc aagaagcaat cttggaatcc catatgatgg aagtagatcc gacgcttgct
    14281 gcgacgaaac caagaatcgt tgtttctcct ttatcaatgg gagatagaga agatccggcg
    14341 cgtcttgtat ttgatgggaa agcaggagaa ggggtagtcg tatcgatggc agatttcggt
    14401 acgcattata aactgctgat caatgaagta gaagcctttg aaccaacgac tgaagcacct
    14461 aatttacctg ttgcacgtgt gttatggaaa actaaaccga atttccatga aggtgtccat
    14521 tcatggattc aggcaggtgg cggtcatcat accgttgtct cgctaaatct aacaacagat
    14581 caaattgaaa cgtgggcaaa attagttgag ttggaaacag tggttattag ataaaaagag
    14641 gttgtgaaaa agccacttag ttttaagaaa taagaagaga ttgacgaaat ttgcttttca
    14701 acttttttga aatttagtct tattatcaat gtcagctaac ggaacaccat ttatcaagat
    14761 atctcaccct caatattttg ctctcgaaaa taaggcttat ggcagtaact tgtcatagcc
    14821 ttatttttat cactcagcct actctgctct aactgctctt tttgcttcta ggaagtcaaa
    14881 tggtaaacag ttgtctaatt gcagaaattt cgagatactt gttagtaagg agggggtttt
    14941 atgtatcacg ttcatttgat tgatgctatt ccagaacaag agatagaaaa gttacaaggg
    15001 gaatatttca aaataataga tgatttgcca gccgaaggaa tagttattcg tagtacgaaa
    15061 gtgaaagatg aatggcttac accagatttg ctagcaattt caagagctgg tgttggggtg
    15121 aacacgatca acgttgaaaa agcttctgaa aacgggacga tcgtcatgaa tacaccaggt
    15181 gtaaatgcta atgcagtaaa agagcttgtt ttgtgctgtt tgctcttatc ttcccgtccg
    15241 ataatcgaag caagtcgtat ggtacaaaca ctaacaggtc ctaatatttt agaacaggca
    15301 gaaaacaaaa gaagtgctta tgtgggacga gaattgcaag gcaaaaccat cggtcttctt
    15361 ggtcttggag cgataggcac aaaagtagcg ctaagttgct atagcttagg gatggatgtc
    15421 ttgggttatt ctatccgcga tgcccagttg gattatgttc ggcaagcaga tttagaaaca
    15481 gttctttcta cctcggatta catcgtagtc atgctgccgt tgacagaaga tacaaaagga
    15541 ttgatcgatc aggcgaatat agaaaaaatg aaaaaagatg ctgtacttat caatgtagga
    15601 agaagtgaaa ttgttgataa atatgctgtt atgcaagcat tggaaaaaca acatctggct
    15661 aaatacttga ctgattttcc agaagaagag ttcttagaaa atgaccgtat acttatgttg
    15721 cctcatctag ggggatcgac tcaggaagct ttggcagaca gcggacgttt ggcagttgaa
    15781 gccttaaaag attatctttt gttcggtacg gttagagaag cggtcaatta tccatccgca
    15841 cgaatgtttt tccaagcgcc gtttcgattc actatatttt atcaaaagaa aaccaatata
    15901 ttagcagaga tattttctct gctgaatgag aacgagctag cgatcgcaga tatatcgcgt
    15961 aaccataaga atgagcatgt ctacatattg attgacatag acagtactga ttttgaacag
    16021 ctcaatcaag tgaaacaaca gctagaaaaa atttctggag tacgaaaggt ccgcttatta
    16081 aagaaaccag aaaggccata aagaaagaga atatttggat ttcattaatt cttctttccg
    16141 ttctttactt tcgaatttcg tacaactaaa ataagagaag caacattctt ttattgatag
    16201 aaatgaagga gcaagcattt gaaaagatat ataagcagac tttcgaccaa acagaaaaag
    16261 attttaaaag gaacagcagc aatcatcagt atcttttttc ttgttgtttt tagtaattta
    16321 tttttgcagt ggtgtcaaaa tgatctatcc gtagaccttg cgttgaaatt tgctttttct
    16381 tggcatacgg aaaaattctt cttagcctgt ctcgtcttgc tgattattct tatttttctg
    16441 attgcactag caggatctgt ccctttaggt tcccttacgt atgtcgtagc catcggtgta
    16501 ttaggttttg caaattatat gaaaatgagt tatcggcaag aaccgattta tccagacgat
    16561 ttgaagatga tcactgaaat cggattgctg aaagatatga ccggcactat gctgtttaca
    16621 gtgatcttag ctgccgcagg aaccgtgata ggattgtttt gctggtacat gtttcgaagc
    16681 ctgaaaaaag gaaggcgatt ccaattgatc cgattaacga cactgcttgt ggcaattgga
    16741 ctgctagggt atatcagtaa tttcaacaat cccgataatt tgttgcgaaa agcatataat
    16801 aaaacggcac tatggatacc ttacagccag aaaatgaact actataacac tggttttatc
    16861 ggtgggtttt tgtacaatct gaaggtagaa ccaatggatg aacctgaagg atattcgaaa
    16921 gaaaagatca aagaaatcac tgaaaaatac cagaagctag ccgatgaaaa aaataaaact
    16981 gtagaggaag agtcgccgaa catcgtgttc gtgatgagtg aaagtttctc agatcctagt
    17041 cgattaaacg gcgtagaagt atctggtgag ccattggcag attattatga agtagctgat
    17101 caaacatata gtggcaatat gctttctcaa aactatggcg gtggaacagc aaatatcgaa
    17161 tttgaagcat tgactggttt ttctatggcg ttatttaatg cgcaactcac caccccttac
    17221 acgatgcttg taccaaaaat ggatcagctt ccttctatcg tttcagcgtt aaacgcacaa
    17281 tcataccaga cgacagcgat ccatccatac aatacttcga tgtataaaag agaggatgtt
    17341 tatcagacat taggatttga ccagttcatc agcgagcgta cgatgaccta tacggatacg
    17401 atagagaata atccatatat ttctgatgag tcagcttaca aagaagtaat gacacttctc
    17461 aaggaagaaa aaacacctca gttcattcat ttagtcacga tgcagacaca tatgccctac
    17521 gatggaaagt atgataatct agactatcct gcaaaaactt cagatggaag tgggacactt
    17581 gacttagaga attatctgca agatatttcc tatagcagca cggcactgaa gcagttcacg
    17641 gaagaattga agaatctttc tagaaggact ttagttgttt tttgggggga tcatcttcca
    17701 gggatctatt cagatacgat ccaagcaaaa aatgacaaac aaacattgca tgaaacacaa
    17761 tttcttatgt tcgacagtaa aggagaatta gaaaagcaaa cgactcacga tgcaatcacc
    17821 agtccgtttt attttgcagc gaatctaatg gaacagacga atcagacaac aaatggcttt
    17881 tatcagctgc ttttatcatt agaacaggaa cttccggcgt ttgaacgaga actgtactat
    17941 caaaacggac agtggtataa agaagcgcaa ttcaatcgct cgcaacaaga gatttacgac
    18001 gaatatcaat tgatccaata tgacattgtc gcagggaaac aatatagttt agcagatggt
    18061 ttctttgagc acgaataaga atggcgagct gtatagaaac cattaaattt tttcaagtac
    18121 ttgtcatttg aggataaaaa caagtagaat aaataataac aacaatggag aaagtaggaa
    18181 aacgaacatg ttaccgaata ttggctatta tgtgcagtcc atcgatcaaa tcgtcaagga
    18241 aacagaagca atcggagaag gaatgcatcc gaaatatgaa gtgatcagaa acgcaattga
    18301 tgaaggaaaa acaagtgaac tggacaaatc gactcttgaa gagatcatcc tgttatttga
    18361 agaaggaaca aaaaaatatc gggcaatgct tatgaaagtc agtactttgc gtccaccagc
    18421 aaaagcaatg ggcattcata aaaattttga aaaagcttat ctttcatatg tagcaggctg
    18481 tgaagaaatg atccaaagta tcgatcttga atcaggggta gatgaagatc tattcaatac
    18541 atctgaagca aaacaagacc aagcgacaga ccaattgaca gctgccatcc aaaaaatgac
    18601 taatttgttg ttgaaaaaat aaaagaaatc tttgataaca ataaaaacta gagttatcta
    18661 atataaatgt aatattacct ttatgataga gtgatcaaaa tcctttatga taatgacata
    18721 cctataacca acaacaacct tgtacttact caaaatcgag taaatactcc ttttccgtcg
    18781 agtctaacca tctcggcggt tttttatttg tagaaaagaa catcaggata gacggaaaag
    18841 tcttgtcctg atgttctttt cagatagtat ggaaagctga ttagttttct acattctgta
    18901 catattgttc aaagtagttc tgtaaatcgg ctttgagttt ctgatattcc tcttcgctat
    18961 actctttatc ttcgattttc ccatcaaaga aaggaatatt tagttcttcc cgtagtctgt
    19021 ctaatacgtc ttgctgattc atttcattct ccttttctta ctaacgatca aaataaacac
    19081 agacacattc tggtacatag acatcttttt tcagtaattc cagtaatcct gtttcaggat
    19141 gacggcgata taatgtcaga ttatcggtgt tctgattggc acacacgaca aaattattgc
    19201 ttggatctag tgcgaaatcg cggggaaaat ccccttctgt tgagatgatt tgcttgtttt
    19261 gaatgtggga accatcttca aaaactgcga atacagcaat cgagttatgt cctcggttag
    19321 aagcataaag gaacttgcca tcatttgaaa tgcggatagc tgcccctcca ttttcaccgt
    19381 cgaattcttc tggcaaggtc gatattttct gtaattcttc aaaagcacct gtttctttat
    19441 tataagaaag cacgcggact gtgctgtcta attcaccaaa taaataagcg aattgtccat
    19501 ttggatgaaa gacaagatgg cgagggcctg ttcctggctc tgcagtgaag gtagttacaa
    19561 gattcaattt gccgttttcg gacacatcat aagtatacac acgatctgta ccaagatcac
    19621 atacgactaa tcgttggtca ggagtcagat ccgtatagtg gacatgcgca tgatcttggt
    19681 ttttatgagg acctgtcgct tcttgatgga atattgaatc tgctgctttc agttcgccat
    19741 ctcctaatat atggtagaca tttacttcac ctttatgata gttcgccccg tatagtaatt
    19801 gacgtgcttc atcaaccgct acataacaca aaggagcgcc agactctgtt accgcattca
    19861 aaaaatgaaa atccttatcg tacgcaccgg caccacctaa tccatccaca ctagtaactg
    19921 tgtaaagatt gccagcttta ctttttgcta cataggtagg gctattttct tcaacagcta
    19981 atgaaaggtt ggctaattct tctttttctg tatcaaggtc gatcgtatag attcctttac
    20041 tttcgcgacg cgtataggtt cctaaaatta tcttttcgat catatgagct gattcctcct
    20101 aattgtaatg aaatagtttt cgtttttatt ctatcacata attattccac acggctaaag
    20161 aatgagaatg tgttatactg tttttgaaag caattaaagg agagagtgca atgattcagg
    20221 caaagattac agaaattggg acagaagcaa taaacaagga agaacccatg ctgattttgt
    20281 tcgataaaac agcaacagct gccttacgaa aatattcggt gattcaagaa gtatcaaaag
    20341 acgaaccttt ttctttgaaa gcaggcggaa caattgcttt tgatgatcag gtatatacga
    20401 tcgattatgt cggtccgatg gccaatggga atttaaagac agtagctcac gtatcattga
    20461 tttttgatgc ttgtccagaa gaaggacaaa ttgcaaatgg aatctatctt catccgcata
    20521 gactgcctgc tattggtttg ggaagccaaa ttttttataa ctaacaggag gaattatgga
    20581 aggaaaaaat aagaagaaca gaccggtatc gtggttttgg cgctggtttt taaataatca
    20641 agtagtcaca gccttacttg ttgtattgtt aatattgctg atcgtctttt tatttacaaa
    20701 agtttcatat ttgtttgcac caatctggca atttttagca atcgtaggtc tgcctatcat
    20761 tttagcagga atcttgtatt acttgatgaa tccggtagtt gattactttg aaaagcgaaa
    20821 agtaccaagg ctttacagta tcattgggct gtttgttttg gttgttgcac taatcgcatg
    20881 ggggagcgta gtgatcatcc ctaagattca ggaacaaaca gtgagcttca tcagtaactt
    20941 ccctcaatat gtagatacga ttgataataa attaaccgag attttgcggg acccgctgtt
    21001 caaccaattt cgcgaacagt tggaaacagc aggtgataag ttcatgaatt cggcaggcga
    21061 tatgatccaa gatatatcta agagtacagt tcagagtctt ggaagttttg tcggcgcggt
    21121 agcgactatt ttagtggctc tgctgacgat gccgtttatc ttattttatc tattgaaaga
    21181 cggtaaacaa ttagcacctt attttgtcaa atttttaccg acacgcatgc aaaaaccgac
    21241 gttgaatgtg ctgagtgaaa tgaatagtca agtttcttct tatataagag gacaactaac
    21301 tgtggctttt gctgtggcaa tcatgtttat gattggtttt gcggtcattg gtttagagta
    21361 tgctgtcacg ttggggatta ttgcgggttt tttaaacctg atcccttact taggatcatt
    21421 tttagcgatg attccggcta tctttttagc cattgtagca ggtccattca tgatcatcaa
    21481 agtactgatc gtttttgcag tagagcagac aatagaagga cgcttgatct ctccattagt
    21541 actagggaat cagttagcaa tccatccagt aacgatttta ttagttctgc tgacttctgg
    21601 gaaattgttc ggaatcatcg gtgtgatctt ggggattcct gtttatgcag cagctaaagt
    21661 agtgattact catgtttttg aatggtacac aacaatctct tctctttatg aagaaaatcc
    21721 gttgaaaaag cagaaagaat aacaaaaaga gacagatggg aaaatatcca aggcacaaat
    21781 gctgctttga agattttttc ctctgtctct tttgtatgca tttatctcaa tttttaaaat
    21841 gatttaccta aagcagcagc tttatctaat gcattttgca tcaactcatc tgcacggtca
    21901 gggtcataat cgattccttc gatgaatagc tgatctactt gatcgatgcc tacaaagttt
    21961 aaaatacctt ttacatattg agaggcaaag tcttgtccgt tgtatacacc gccattagat
    22021 tggatgtgca gtgctttttt acctgtcact aatccttctg gcccgttttc tgtatagcgg
    22081 aacgtttttc cagctacatt gacggtatcg aaccaagctt ttaattttgt tgggatattt
    22141 agattccata aggcgtttgc gataacgatt ttatcagcag caaggaactg atcagtcaat
    22201 tcattgaaac gagcaatttt ttgttgttga ctttctgata aggaagtgaa ttctgcacca
    22261 gcacgtaatg cttcccaacc tgataataat tcttcatcga tttccggaat atcatcgcga
    22321 taaagttcca aaacagtcac ttcatcatct ggatgatttt ctttataact tgttaaaaaa
    22381 gacgtcaatg ccttaactgt gcgcgattct tctgcagtta ggggatgacc tttgactact
    22441 aaaacagatg ccatatgctt accttctcct tttattgcta tgattgttat aactatgact
    22501 aacaaaatca gtttactcga aaaagaaaag gctatcaagt catattgata aggttgaaga
    22561 ataaataact aaaaaaagac agggtataaa agaggctgtg acacgatttt ttgtcacagc
    22621 ctcttttgaa tctaacaagc aatattcaag caacactcga ctgaagagct tccatcccac
    22681 caatgatata aacagcacga aaaccattag cagtaagttg atcggctaat gcttctgatc
    22741 gcctgcctgt atagcttaga atataataaa gtttctttgg atctagattt tttaattgtc
    22801 ttggcaagat agcagcgggg atagaaagcg agtggcttaa gtggtttttt gtgtaatcta
    22861 aggtatttct tgtatctaaa acgatgctgt cagcatcgag aggcaattta ttgaagtctt
    22921 ggacactaat tgattgggcc atttatctca tcctttgttt agtatgtggc tattttaatg
    22981 ggcaactctc attaattcaa atggaaggat tcctcttatc acttttttaa tgtatcggat
    23041 gataatgaaa aaaactatgc aaatgtcttg caaaacaggt aggaggtgtt ataatgacat
    23101 agcgtcatat gacaatgtgt catcttgaaa gaaaggagaa tccatatgcc gacacagaca
    23161 tttttccatt tgcctaaaga aaaacaaaaa agactaatcg aggctgcgag aatcgagttt
    23221 tctagagtac ccttaaaaga agcttcgatt gctaatatcg tcaaattagc ggatatccct
    23281 agaggcagtt tctatcagta ttttgaagat aaagaggatc tttattttta ttactttgag
    23341 acaatgagac gtgacagctc taaagatatg ttacaactaa tgaaagaatc cgatggtgat
    23401 ctattcaaag gattcgaatc ctatttttct aaaatgattt atgagatttt ggaaggcgag
    23461 aatgcttctt tttatcgtaa cttgtttatg aacatggatt accgtgcctc aagaaaagtc
    23521 gctcctcata cgagtcgcca aaaaacgcct cacgatccac ataaagaaca caaacaagat
    23581 gcaaaagagt tgcttgaatt gatcgatcgg acgaagctga aagtaaaaaa tgatcatgaa
    23641 ttggaattac tgatccaatt agtgatgaat actgtattta caacaattgc gcattcgtat
    23701 cgcttatttt caacatctga agattactca gtagagcaag caatcagtga attcaatttg
    23761 aaactttctt ggctgaaaaa cggagtatat aaatagaaag aagggaacta gatgctaaaa
    23821 atcgtaaaac ggatctcctt gacttcagca attgcagctg cagttttcat ggtgatccaa
    23881 gtactggcag atttatattt gccgacactt acctccaata ttattgataa aggggtagcg
    23941 caaggagatg tcgattatat ttggcataca ggttttgtga tgatcggctt ttctttgatc
    24001 agtatcttag ctgctatcgc aaatacattt tttgctactc gtgaatcaca gaaactgggt
    24061 aagcaattgc gtacggatgt ctataaaaaa tcagagagtc tgacaaaaga tgcgtttgat
    24121 aaatatggaa cggcctcttt gatcacaaga acgaccaatg atgtgactca aatccaaatg
    24181 gttacacaaa tgtttttacg tatgatgatc aatgcaccaa tcacgttgat tggcgcgagc
    24241 atacttgctt atcaaaaaga tcatcagttg acaaaaatct tcttagttgt catcccagta
    24301 atgattattt tgatcggcgg aatcatgtac tttgctgttc cattattcaa aagtatgcaa
    24361 aagaaaactg accgtttaaa cctggtattt cgagaaggat tgacaggggt cagagtgatt
    24421 cgagcattcg ataagacacg ctttgaagaa aatcgttttg atttagcgaa taaagattat
    24481 acaaatacgg caatcaaagt taatacgatc gtcgcattga tgatgccaat gatgacattg
    24541 atcatgagcg gtacaaacgt agcaatcact tggtttggtg gacactacat agcagatatg
    24601 actttggaag tcggtaatct gattgcattt atgacttatg cgatgcagat cttgatcagc
    24661 tttatgatgc tttccgctat ttttatcatg gtgcctcgtg cacaagcttc agctgaccgt
    24721 attaatgaag tgttggatga aaaaatcggt atccatgacc ctgaaaatcc taaaacagtt
    24781 tcttttgctg gaaaaaacgc aacattggcc ttcaatcatg tcaattatcg atatcatgga
    24841 gcagaaaaac ttgcattgga agatattgat ttccaagcca agtcgggcga gatcgtcgca
    24901 atcatagggg ggactggttc aggtaaaacg accttagtca atttgatccc tcgattatat
    24961 gacatcgaat ctggttcgat ccaaataaat ggaacggaca ttagtgacat gactcaatat
    25021 aatttgcgag aattaatggg ctttgttcct caaaaagcag ttctgttctc tggaacgatc
    25081 cgagacaata tgcagtatgg taagccggat gcaacagatg aaatgatttg gaaagcatta
    25141 gaaatcgcac aagctaaaga ttttgtttca gagatggaag atggcttaga tagccatgtg
    25201 gaacaaggag gaggaaactt ctctggtgga caaagacagc gtctggcaat tgcacgggca
    25261 ttagtcaaaa cagcagatat ctatgtgttt gatgattcct tctcagcgtt ggatttcaaa
    25321 acggatgcca atcttcgcca agcattgaaa acgaatatga aagaaagtat caccgtttta
    25381 gttgctcaac gagtaagtac agtaatggat gcagatatga tccttgtgct agatgaagga
    25441 aaactggttg gcaaaggaac acatgaagaa ctattagcaa caaacgaaac ttatcaagaa
    25501 atcgttcatt cacaactaag agaggaggac ttagcatgag taaaacaaac acacgtccct
    25561 ctcatggacc agcaggcggt ccaggacatg gtggacctgg cagaaatctc ggtgtaaaag
    25621 gtgaaaagcc taaaaatttc tggggaacgg tacgccgatt gtttggctat atgtctaaac
    25681 gtatggttgc gatcatcgct gtatttgtac tagcaatagc agccgtgatc ttccaaatcc
    25741 aaacaccaaa ggtcttgggg aaagcaacca cagaaatatt caaaggtgta atggctggcg
    25801 ctcaacagat gcaacaaggc caacaagtaa caaaatttcc gatagacttt gataaaatcg
    25861 cgcagatcat tgcgacagta atcgtgatgt atttgatttc tgctgtattc aactttttac
    25921 agcaatttat tatgacacgt gtttcccaac gaacagttta tgaattacgt catgatttgg
    25981 aagccaaaat gaatcgtgta ccgatttcct attatgatac acatacaaat ggggatatca
    26041 tgtctcgagc aatcaatgat atggataaca tcgcaagcac gctgcagcaa aatttgactc
    26101 aatttgtaac tagtgcagtc acattgattg gggtcatcgt gatgatgctt tcaatcagct
    26161 ggcaattgac gttagtcgcg ttattgatga tccctttaag cttgatcatc gtgatgatcg
    26221 tagcgccgaa atcacaagtg tttttcgcag atcagcaaaa aagtttaggt ttattgaata
    26281 accaagtaga agaaacatat ggtggtcata ccattgttaa aacattcaat catactgaaa
    26341 aagaccagga agtatttgaa aaagaaaatc agaatctgta tgccgctggt tggaaagctc
    26401 aatttatttc ggcaatcatc atgccgttga tgaatttcgt taaaaatcta ggctatgtgt
    26461 tcgtcgctgt actaggcgga gtaaaagttg caaatgggaa catgacatta ggggatgttc
    26521 aagcgttctt gcaatataca acacagttct ctcaaccaat cactcaactt gcaagtttga
    26581 tgaacactat ccaatcaaca gttgcttcag ccgaacgggt atttgaagtg ttggatgaag
    26641 aagagatgtc caatgaaaaa tctggccttc cagaagaaaa agatacacca tacaaagttc
    26701 gttttgaaaa tgttcaattc ggctacacac cagataactt gctaatgact gattttaact
    26761 tggatgtcaa acctggtgag atggtcgcta ttgtaggtcc gactggtgca gggaagacga
    26821 cactgatcaa ccttcttgaa cggttctacg atgtttctgg aggtagtatt cgttacgatg
    26881 gtgtagatac ccgtgatctt tcacgtgatg aattgcgcgc acagttttca atggtactgc
    26941 aagatacttg gctgtttaca ggaagtatct atgacaatat caaatatggg aacgacgatg
    27001 caacagacga acaaatcatt gaggctgcta aagctgctca cgtagacgat tttgttcgta
    27061 aattgccaga aggatataac actgtattaa acgaggatgc aagcaatatt tcgcaagggc
    27121 agcgtcagct aatcacaatt gcgcgggcat tcttagcaaa tccagatgtc ctgatccttg
    27181 atgaagcaac atctagtgta gatacacgaa cagaaatact gattcaaaaa gcgatgaacc
    27241 gcctattgga aaaccggaca agtttcgttg ttgctcaccg cttgtctaca atacgcgatg
    27301 cagacaacat tattgtgatg aatcatggtt cgatcgtaga aacaggaaac cacgatacat
    27361 tgatggaaaa agacggattc tatgcagatc tttataacag tcagttttct gaagaagaag
    27421 cttcatagaa tagtacaaat ttattatttt ttttaggggc tgtgacaaaa gtcgtgtcac
    27481 agtccctttt tccgaataaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa
    27541 cggcaaaata tgagaagcta tttttccgtt ttgtatctct aatactcagg tcaaaccgct
    27601 gattttacaa cctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
    27661 atagtcaaca aacctgagaa gctgtttttt gaaattgctc cttgtgactc ggagttaaac
    27721 ggcaggctca caacctcttg gtaaacggtg ttcaaaagcc agtttctcgg tattaagcca
    27781 aatttatgca aaaatgtaga aagccatttt cacaaaattt ttcttactac tcgaaactaa
    27841 gcgacttttt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    27901 gcccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
    27961 cagaacagct ttttcacaac ctctttttat aagagttttt actcattaat ttacaaaaaa
    28021 caagcaaaat gcgaattttc tcacattttt tttaacaaaa tcaaagttat tgcttgaaag
    28081 aagaagaatc catcgttaaa ataaagcttg acgtagaggg gagcaagaaa tatgaaagat
    28141 tttcaattgg gggctgccac ctatttaaaa agtgtagcca ttcgggtgaa agaccgagat
    28201 aaaatgattg atttttacaa gaacgttgtt ggctttgatt tgaaaagaga agaaaacgaa
    28261 ttagccattt taggaacaga agaagctgga agtgaaatgt tgctgctgga agaaagtcct
    28321 agagcgaatg atcacatggg ggtgatcaaa aagctgaacc gtttttcatt gattatccct
    28381 agcacagaag aaatggcaga tatcctttgt cgtatccgta aaatgaatta tccaatcaaa
    28441 gaagctttag aagatcatgg acgtatgggg attttgctcg tagacccaga agagaatgaa
    28501 ttggaaattt accatgaaga gaataagaac gaccaaagag aaattccaga aacaatggat
    28561 caagaagcat tattaagaaa atctgaagaa aaattcagta aactatcaac aggttcttat
    28621 tttgaaaaaa tccatttaaa tgttactaac ttaacaaaag aacgccaatt tcttaatcat
    28681 gtacttggtt tgaaggtaca taatgaagaa gagggcatcc atgtattgaa tgaaggagat
    28741 ttccatgttg gtttgacaga agctagcgga ggcgcaattg atttgcctac agatgaagtg
    28801 attggacttg attttctgaa attcatgatc gatactgaaa gcatggaagg actgattgct
    28861 cacttgcagc aactatcagt tgatttcttc ttagacaaaa agcacacagt actcacaatc
    28921 tatgatgcta taggaatcga atggtggttc gtcagtgagc caaaagaaaa gaataaaact
    28981 gacattcgat gcgacgctta actttttctt tcttaacgat tgtcgcatgt ctcgtcattt
    29041 tatgaacggt catactatcg cttattctag agatagaaag agaaaatagt ataccggggg
    29101 aaactgcgcg gtttttcgcg cagttttttt gtgtaaaaaa ggttgattcc tcatagatta
    29161 agctttaagc ttggactgta agaatctgat agaaaagcag gagaaaaaga tgtatccaca
    29221 cacgaaacag cttattttga aaaagatgga agaaggattt tatccaggtg tagcagtcgc
    29281 cttcataaaa gaaaatcaag tagaaacatt tacagccggc tttgcccaag tcgttcctga
    29341 aaagcagctg atgacagaag atctgttgtt tgatgcagcc tcactgacga aagtcgttgc
    29401 aaccacttca ttagttttac gtctattaga agatggaaag atagaactcg atcaaccgct
    29461 tcataaatat cttcctgaat ttgaaaatca gacaatcact ttgcgtcatt tactgacaca
    29521 tacttctgat atcaaaacat ggataccaaa cagagataag ctatctcagg aagaattgat
    29581 cgccgcttat ttgaagctag aaccaggtga gcaactagga aaagaagtaa aatatacaga
    29641 tgccggtacg attcttcttg ggttgatgct agaggaaatt taccaagaaa ctgcagtgga
    29701 aatctttcaa gaacaagtat tggaaccatt aggaatgatg aacagcttat ttcttccttt
    29761 tccttccaaa aaaatcgttc ccacagaaca actgccttcg ggtgaggttt tgcgtggaca
    29821 aactcatgat ccgaaagcaa gagttttggc tgaacatgca ggtaacgcgg gtctctttat
    29881 tgatttgaaa gacagcatta cttttgtcca aatgtatttg aacaagggaa aaatccgtaa
    29941 tggtcgtttt ttagaagaag agacgattga ttctttactg caagatcaaa caccgaaaaa
    30001 aaatggctat cgttctttag gatgggactt aaaaaaggat gtagaagacg gtcatgcgat
    30061 tctttttcat actggttata caggtacgtt tttcttgatt gatataaaaa cacaagaagc
    30121 gttgatattt ttgtcaaatc gcgtacatcc tgtcgatcat cgtgcggagt atctcatatg
    30181 tcgagatgaa attgttgaaa actttttgaa agagaaagcg gtattaccga aataatgcta
    30241 taatagctat gaaaaatgag aggagtttga attatgcagg aacctatgtt tttaaaacca
    30301 gtttttcaag aaaaaatttg gggaggaagt cgtttacgtt cagtctttgg ttttgatatc
    30361 cctaacgata aaatcggaga agattgggcg atcagtgcgc atcctcatgg tgtcagtgta
    30421 gtagaaaatg gcgaattcaa agggaaaaga ttagatgaac tatggtcaga acacaaggaa
    30481 ttgtttggcc atccttcaga accagtgttt ccattattga tcaagatctt agacgcagaa
    30541 gatgatctat ctgtacaagt acatccagat gatgcctatg ggatgaaaca tgaaggtgaa
    30601 ttaggaaaaa ctgaatgctg gtatatcatc gatgctgaac ctggtgcaga aatcatctat
    30661 ggtcatcatg caaagacaag agaagaacta gctgagatga tcaaagatgg gcgctgggat
    30721 gaccttttga agaaagtacc agttaaaaaa ggtgatttct tttatgtacc aagcggaacg
    30781 atccatgcta ttggtaaagg aatcatgatt ttagaaacac agcaaagttc tgacacgact
    30841 taccgcgtgt atgactacga cagaaaagat gcgcaaggac aaacaagaga gctgcatatc
    30901 caacaatcga tcgatgtaac aactgtccct gcgaaaacac cagaattaca aatcaaagaa
    30961 gtccgaaaag ggaattcatc gatcgtcact tatttagaaa ctgagttttt caatgtatat
    31021 gagtgggata ttaaaggaat cacatctttc aaaaaacaag ccccttatac attgatgacg
    31081 gtaatcgaag gagcaggaga ccttgtcgtt gacggaaaaa catatccatt agaaaaagga
    31141 acaagctgta ttattccaaa tggcgtttct gagtgggcgg tccaaggtga actagcaatc
    31201 attgcgtctg aaccaggcaa atagttccca gatagctgaa agcagcagac ggtttttcgt
    31261 ttgctgcttt ttctacataa aatccgtcca tcaataaata gaagtctggt gaaaaagatg
    31321 gaatacaaat ggaaagcaaa caacagcagt cgcgtgtcta tgaaacaact gagaaaagct
    31381 ggacatcact taaatgaaat gatcatgcca aatctaagtc tgtttatcgc ttggggaatc
    31441 ttgtcattag caacaaaagg tgtgtctggc gatttgcaca ccacattaga agaggtatgc
    31501 cattggatga tccagctgct tttacccatc ttgatcagtt attcaggcgg gaaatcggta
    31561 gggggacaaa aaggggcagt cgccggtgca attgcttctt tagggttgat cgctcgttcc
    31621 aatgctccac aaatcgtagg agcgatgatt atcgggccgc ttgccggcat cgtatatgaa
    31681 caatttgttt ctagattcca tgtgaaattc aaggctggat atgaaatgct tgtcagcaac
    31741 ctactagtag gactgactgg tagcgttatt tgtataattg gaatcgtttt tattgaacca
    31801 gttttaaata gtgttcactt gttgctcgcc tccgttgtgt cttggctagt cacaatgaat
    31861 ctgttaccac ttgtcagtct gatcttagaa cctctaaaag tactattttt caataatgca
    31921 atcaatcatg gtgtattgac acctttagga atcgaattct cacaaacagt cgggtactct
    31981 tccttatttt tgatggaggc aaatcctgga cctggtttag ggatcttact ttctatcctt
    32041 tgttttgcag aaaaacagga aaaagtaaat gcgagtggag cattgatgat ccatgcgatt
    32101 ggcgggattc acgaaatcta ttttccattt gtcttgctgc gaccgtatct atttttagct
    32161 gtaatggctg gtggggcaag cggcacgttg atatttcaat ggttagatac gggattagct
    32221 gcacctgtat cgcctgggtc actactcgtg atttttgcca atgcgcctta tccacaacta
    32281 gctggggtaa gtatagggat agcaatcagt acatttgtga ccttcatttg tgcagcgata
    32341 ctattgaaat taccaaataa agaaaaagag aacaaaacgg tcaaagaaag gggagaaatc
    32401 ttggaaccat ctatcattca ttccgtcatt tttgcttgcg actcaggtat gggatcaagt
    32461 gctatgggag ccgccctgct gaaaaagaaa ttagcagcag aaaaaatcga tctttctgtc
    32521 ggatacagct ctatctatga attgaataac caagtaaatc agctggttat cgtgcaacat
    32581 gaactagcgg gtcttgcaag agaaaaagta cctcaaagtc aaatctatgt actggatcac
    32641 tttttggaaa cagatatctt gctgtcacga ataaaagaag cgatccaatt tccaaagcgg
    32701 gaagaaaaaa aagagatagc agatatcaca gaacaaaaaa cgtcgcctgc taatattgta
    32761 tttttgtata aaaacaacag acgcggctca caaactatgg gcatgacgat tttaagtcaa
    32821 ttagcaaaaa aagcgaaaaa acagtttgtt atctcaaaag aatcaataga aaaaatcaaa
    32881 atccaagaag aaacgattta tatcgcacaa agagaactag ctgtaactga acagctagaa
    32941 gaaaagatcc ctcggctagt tctagttgaa cattgggtag ctacgaatga atacgaaaaa
    33001 ctcgtgaagg gagaaatata agatgtattt atcgcccaga gaaaaacaat tattagcaga
    33061 atttctcagt agcccgtcac ctgtatctat ccaaaaaatg atgaatttat tgaaggtcag
    33121 taaacggact atttaccgcg aattagataa tcttgaagtc tcattgaaat cggtaaatgc
    33181 ttctttgaaa aaagcagcta gaggctcatt tgtcattgag gcatcagaag aagtattaga
    33241 gcaattaaaa gaaaatacag atgggaaaag taatgactta tccactgcta aacgacaaca
    33301 cgcgatttta gccgagttgt tactgacgac agagccgttg agtcttgctc atttcatgga
    33361 atcatatcgc atcagtaata ccacttttta tgcagatatc aaacagttgg aagaaagtat
    33421 tcgccaactg cctttggaaa tcattcggaa ccagggatac gagatcgaag gaccggaaaa
    33481 ataccgccgg cttctgacag cgaatgtatt agaattagaa atcaatgaat atgaactttt
    33541 tcatagtatt tctttcgatg catcactgaa ttattttttt cgattcgttg atccgcaaca
    33601 tctatctctt gcacgcaaag taatcgggga agaattgatc caaaaaaaga ccaatctaag
    33661 tgatagaaag cttgaacatt tggttttgat gctgacattg acgatggagc gtgtgacgaa
    33721 aaatcattta ctgacaaatg agacatataa tagtctggca aacaaagaat tactacagac
    33781 agctaaacga ttgttttcac ggatcgcttc tgagacaaaa caactttatc cagtaaatga
    33841 gatcgtcttt tttgcaggat tattaaatga cttcacgaac tcatttgatc agaatttctt
    33901 tgaagagacg ttcgatacac agcttgctta tttagtaaaa caactgattc aagaagtaag
    33961 tgaagagacg aatgtccatt tttatgaaga tgaaactttg tacaaaatgc tgttgactca
    34021 tttatctggt gtgttttcaa gagccgtttt acaagaagag acactgacca atccgatttt
    34081 agaaaaaatc atggttcaat acgaggaagt tgcgaatgca atccgtaaag cagtagagcg
    34141 gatttttcaa gaaaaaaaat tatccgaaga agagatcgct tatatggtcc ttcattttgc
    34201 gaattcattg gaacgaagtc cgaaagaaag caatatcagt attgcaggat tttcgccaag
    34261 tggattagct tctactagca tgttggaact acgattgaaa aggtattttc ccttcatcca
    34321 acaaattcat ttctatcgga ttgctgattt aaaaaaaatc gatttacaag aagaatatga
    34381 tgttgttatt tctacatcta ttttaccagg atacggtgga aaatatattt tagtctcccc
    34441 gctactgcta gaagatgaag tcaagcagct aaaagaagct ttttcgcata ttgagaagaa
    34501 aaaaaggcaa aaaattacgt ataacagcaa taacgagtgg atgatagaag atacttatga
    34561 ggaaacaatg catttcatgg atcaaatcaa tcaattactc ggtcgttttt ttatccaaga
    34621 actggataat cctgagacga tttccgaaac gattcgcagc ttatttcctt attttcctga
    34681 aggactagta acggatgaag aggtggttca tcgccggctg cttaagcgat atgaacaagc
    34741 acctatcggt attcctcata caaatatggg gttattccat acatccagct cattgatttc
    34801 taaaccgatc ttttgtattt tcaatttgaa acatccgttg accattgaag ggatggatag
    34861 acagccagtc gatttgtccc gtatgttagt tatgctggcg ccttcgccaa tccaagaaaa
    34921 agacgggatt cttttaggaa agatcagtgg agcaatcatc atgaatgact tgaacaccga
    34981 gatcttccgc tcaggaaata aagaaatcgt ttatcagttg ttagcgaaaa tcctgattga
    35041 agaaataaag aaatgaaggt gaaaagatga tacagctacc agcagaaaaa atattgctcg
    35101 atcaatccta tcaaaattgg aaagaagcgt tgaccgagat cggccagtgg atgacccaac
    35161 aagggggaat caaaccggca tatatccaat caatgatcga aagacaagaa aaagcgagtg
    35221 tctatatcgg gaatttcgta gccttgccac atgggactga caaagatgaa gaagtagtag
    35281 aagaaggaat ctatttagtc caagtgcctg atggtgtgga ctttggaaca aaagaggaac
    35341 caaaaatcgc gacactctta tttgtagtgg ctttaaaaaa agaacatcag ttagcggtgc
    35401 tccaagattt agcctttttc tgttcagaca tcgaccaagt aatggcgctc agtgatgcgg
    35461 aaaccattga agaagtccaa atgatttttg aacatggaca tatataacca atgttcacag
    35521 taaaaaaagc tgccttgcat caagtaataa cttcaaccaa gataaaaata agaggagcta
    35581 tttttgttgg gaggttctta ttactgcgga tgcaagcttt tgaaaactat ttattctaga
    35641 aaaccgttta tttgaaatag gggacggtga caaggttttt gtcaccgccc ctattatttt
    35701 tgtgttttaa tagctctttt ttatatagat agtgcccata cctatgaata ataaaacgat
    35761 actccatata ccaagtgtca agataccatt catgaaagta acctgttctg aagctagtgg
    35821 cgagaaaata tctcctccta tgatcacatt ttgaaaatca acataagaag ttggcaagta
    35881 atgactgatt gattctatcc atccaatcga agaaaacaag cgatactctg ctaagaaaag
    35941 acaaagaagt gtacctaaaa gattgactag agaatgttca aataaagcag atagaagaaa
    36001 actgattgta cttaaaaaga gcacccacaa caacaggaac agaaagctct ggaaaagata
    36061 ctcatacatc ggcaggattc gtatctcctg accaatgatc gttcctacag gataacggaa
    36121 gtctcccaaa ccatttttga ttattactag tatagagacg atcaaaaatg gcatcacgag
    36181 acttagcata gctgcaccat aataaatggt aaaacggatg ctatgtttct ttaccatact
    36241 atccagtaat agattgacta gagagattac ctgctttctt ttttcataag agatgatgga
    36301 agaaagaaac aaggcggtta tacataaaat caaaaaacta gaaatcattc ctgaaaaaat
    36361 attttcgtag taatttgcta aagagagatg cgctggcaag tcaaaaatag aatatctttg
    36421 gatctgatgt tctttcatat aacggagcaa ctcgacacgt ttttgttgtt caatgattgg
    36481 aattccttgt ctttgaccag aaatcagtcg atccagtata tctttttcat aatgataagt
    36541 tgcttcgacc atttgctgga cattccctgt gttttttgca tgtaagattg cttcgatcag
    36601 tttgttgctt ttctctaaac tttccacaaa ttctttctca ttctcatctt ctttcaacgt
    36661 ttcaatcaaa cgttgcatct cttcatagtt atgattcagt tcatcgaatt ctgtataagc
    36721 aagtttatga tcaaaaaaaa gtgggaatgc aaagagcacg accaacaata gaggaaataa
    36781 aaagtttact ttgtttttta gtaagagatt gcgttcaaat gtaagtaaag acatagttac
    36841 acctcatata atttacgata aatagctaaa atctcttcca tatttgagga ttctgtgacg
    36901 atttctccat tttttagaaa acatgcttta gaacaaatcg attggatatt cgcgatagcg
    36961 tgggaagaaa gaagaatcgt cttcccctct ttctgccatt gtctgatgag gctattcaag
    37021 atatcaatac ttcctgggtc taatccgttc aacggttcat cgaataacat gacagatgaa
    37081 tcactgatca agtaagcagc aagcaagaca tgttgtttca ttcctagaga catttttcca
    37141 atcggcaaag taaggtactc agtcatcttc aatcgttgga aaacagtttg gcattctact
    37201 ttgctctgcc aatagtattt gatcaaagtc aaataatcaa cagccgatag atagtcggat
    37261 aactgattcg ttgattctag aaaaaagaaa gaccggcgat aatctgtata agcagtagga
    37321 gagatgttgt gaagggacaa ttgatcataa gttgttggca gcagttgggc aatgccattc
    37381 agcaaagttg ttttgccaaa tccgttcggg gcaatcaaag cagctacttc tcctgattct
    37441 actaaaagat tcaattgtcg aatcacacgt tttttaccac gtttcatgtt catgtttttg
    37501 atcagtaatg tcatagatag gcctccaata taaaatttaa taacataata gaatggaaaa
    37561 catctaaatt aaatgctaac aaaagttatt gtttgcatat tataaaaaaa ttaaaataac
    37621 agttttattt aaaattgaag ttttctgata ccgaaatctt tttattttgc tgacatactc
    37681 aaacctttgg cacaaagttt cgtgccaaag tacgactatt tctcttttta gtaagcggat
    37741 acaattagag tatcaaaagg agagagggag agacaaaatg gaacggacga ttcccaaaaa
    37801 caacatttcg ctaaaagcga gggtacaaaa attaggcagt actttatcaa gcatggtcat
    37861 gccaaatatt ggagcactga ttgcctgggg tgtattaacg gctctattta tcccagatgg
    37921 ttatttacca aatgaggctt tcgctaccat ggtaagccca atgctaactt atctgattcc
    37981 tttattgatc ggttatactg gcggaaaagt aattgctggt gaccgcggat cagttgttgg
    38041 ggcaattgca acgatgggtg tgattgtcgg aacagatatt ccaatgatgc taggcgcgat
    38101 gatcatggga cctcttggtg gatatgccat caagaaattt gatcagctat tccaaaaacg
    38161 gatcaaatcc ggttttgaaa tgctagtcaa taatttttca gcaggtctta tcggttttgg
    38221 attagcgtta ttaggttttt ctgcgattgg tccagttgtc gatgcattga ctcaagcaat
    38281 ggcaaaaggt gtagagatca ttttatctgc ccatttgatt ccattgacaa gtatttttat
    38341 tgaaccagca aaaatcttat ttttgaataa tgcaatcaat catgggattt tgacaccatt
    38401 gggaactgaa caagtagcag aggctggaaa atctattcta ttcttgcttg aagcaaatcc
    38461 tggtccagga cttggcgtat tacttgcttt tactttgttt ggcaaaggag cagcaaaatc
    38521 ttcagcacca ggagccatga tcattcactt tttaggcggg atccatgaaa tctattttcc
    38581 ttacgttatg atgaaaccgt tattattctt atcagttata ggcggaggaa tgtcaggaag
    38641 tttcattttc caattgatgg atgctggact aagagcgcca gcttctccag ggtcgatcat
    38701 tgcgattcta gcgatgacac cgttcaattc ttaccttcct gttattttgg gcgtgattgt
    38761 tgccgcagct gtttcgtttg gtatttctgc agtaattttg aaagcggatg cgaaagatac
    38821 aaatgaagac ttcgaaaaat ctgttcaaga gacaaaggca gctaagcaag cttccaaagg
    38881 tgcccaaggt attcctcaag gagctacatt ctctggcgta caaaaaatca tttttgcttg
    38941 tgatgcagga atgggatcaa gtgcgatggg cgcttcgatc ttgagaaaaa aagtacagga
    39001 agcagacttg cctcaaacgg tgaccaatca agcaatcagc caactgacag atgactcgaa
    39061 tacattgatc gttactcagg tcgaactaca agaaagagca aaacaaaaag caccgagcgc
    39121 gcgttttgtc tcagtagaaa actttttgaa ttcacctcga tatgatgaaa tcattcaaga
    39181 attaagcagc aagcagaaaa catcagtttc tgaaattgag aatgagccag cagaagagtt
    39241 tctttcagca aacatcaaac aaatcaaaga gattctactt gtccacgatg atcgttccgg
    39301 atcagcaaca atggggatga ccgtgctcaa agatatatta gacaagaacc atttaactat
    39361 cccgttaaga aaagtcaata tccaagattt aaaagatgaa cctgattcac tgatcgtagc
    39421 caaagaaagt ttagcggaaa aagtacagat ggctgcacct caatccattc atttatcagt
    39481 caacagcatg gtcaatccgc aaaaatacga atcgattgca caatatttga aacagacagc
    39541 ttaagggagg aaaacagcat gcaaagtttg ccattcgaca tgatcgagct caatcgatct
    39601 tttaaaacca aagaagaagc gatccgctat tgcggtcaga tgcttgtaga agcagggtgc
    39661 gttgatgagg aatatatcga tgccatggta gatagagaca aaatgttatc tgtttatatg
    39721 gggaatttta tcgcgattcc tcatggaaca gatgaagcaa aaaaatatgt gaaaaaatcc
    39781 ggcatctgtg tcgttcaagt accagatggt gtagattttg gtaccgaaga agaagaaaag
    39841 attgcgactg ttttgttcgg tattgcagga gtcggagatg agcatttgca acttgtacag
    39901 caaatcgcac tttattgcag tgatatggac aatgttgtcc agctagcaga cgcgctcagc
    39961 aaagaagaag taacggaaaa tctagcaatt gcttaaggag agagaaaaat gagagcagta
    40021 cattttggtg caggaaatat cggtcgagga tttattggag aggttttggc agctaatgga
    40081 tttgagatta ctttcgtaga tgtgaatcac acgatcattg atgctttaaa tgaaagaaaa
    40141 gaatacgaaa tcgaattagc agatgaatca aaagaaaaaa tcactgtaaa aaatgtcaaa
    40201 ggaatcaaca atcaaacaaa tccagaaaaa gtaatccaag aaatcgctga agcagattta
    40261 gttactaccg ccattggccc gaatatctta ccttttattg ctgagttgat tgcaaaaggt
    40321 atccaagaaa gagaaaagga aggaagcaca acatctttag atatcattgc ctgtgaaaat
    40381 atgattggcg gttcagcatt tctagaaaaa gaagtgtata aatatcttcc ggaaacatca
    40441 tttactgaca aatatatcgg ctttccaaat gcagcagtcg atcgaatcgt cccattacaa
    40501 caccatgaag atcctttatt cgtccaagtg gaacctttca aagaatgggt cattgacgat
    40561 agccaaagaa aaaataaaaa aatccaatta aaaggggttt tatatgtaga tgatctagaa
    40621 ccatatatcg aacgcaaact atttagtgtc aatactggtc atgcgactgt cgcttataca
    40681 ggtgccttgt taggttacca gaccattgat gaagcaatgc aagatgcgtt agttgtggca
    40741 caattaaaat cagttttgca ggaaacagga agtttgctga ttgccaaatg ggggtttgat
    40801 gcagagcagc atcatgcgta tattgaaaaa atcattcatc gcttccaaaa caaaaatatc
    40861 tctgatgcga tcacacgtgt cgctcggaca ccattacgta agttaggtta tcaagaaaga
    40921 tttacacgtc cagtgagaga actacaagaa cataacttga cttgcccaca tttgacggca
    40981 acaatgggga ttatttttaa ttactatgat cctgaagatg aacaaagccg tcaattgcat
    41041 gaaatgaaga tccatgaaaa cttggaacaa ttgattcaag aagtcacagg aatcaatgat
    41101 ccgaaaacaa tcgggaatat caagcaaaac gtgaaccgtt atgccaagca agtcgcttaa
    41161 aataggatac gaaaaagggg aatgtgacga aatttttgtc acattcccct ttttcgaata
    41221 actaggctgt ttgtccttca agatagggca gatccaattc tttaccgtaa cgttgttcta
    41281 aagctaggcg gatcaaacga gctgccaggt tctcatttct tgcaaggata ggtccatgga
    41341 aataagaacc gaagacattt cggtaaatga caccttcaga ttggtcttca ccattatttc
    41401 cttgaccttg taccactttc cccaaaggtc gttcaccttc acctaagaaa gtccgaccat
    41461 tgtggttttc aaatccataa taagtttcat tgaattcttc gttatggata acgatatcac
    41521 cgataaaccg gttattttcc tgacttaacg tgtagtgatc cagtgcactg attccttgga
    41581 tcttttctcc attagctccc atgtagtagt gacctagcaa ttggtagcca ccacaaatgg
    41641 ctaacatgac gccatcattt tcgatatagt tgatcaaaga atctttcttt ttctggatgt
    41701 cttttgaaat gattaattgc tcaaagtctt gaccaccgcc aaagaaaacc aagtcatatt
    41761 tatcagcatc aaaaggttca tgtatactaa cgatctccgt atggcaagaa acccccattt
    41821 tttctgctat atatttcaac atcaaaaggt ttccgttatc accatatgtg ttcatcaaat
    41881 ttccataaag gtgggcgata ttgagttcat aattagccac ggttcattcc tcctttgata
    41941 aatccttgag cagctagttc tttacgaagc tgcaatacag cagtgtaagt agctaaaata
    42001 tagacatgct cagttggcaa ttctttgatt ttttcgatta cttctggtaa ttcttttgtt
    42061 tctgtcaatt tattttctgg aatcccggca acttttagac gtaaagtcat atctgtatga
    42121 cggtctcctc cagaaataac agctgggata tccatatcgg caaagttttc aaaattacca
    42181 tcccaaatcc agctgacatc gattccgtct gcgtaattgg catttaataa agccactaaa
    42241 gaaaatggat aaggagctag accaatcata tcgattactt gattgatacc tacaggattt
    42301 ttgacaagta ctaatgtaca ttttttccca ttgatattga tgacttcttg tcggccgaat
    42361 actttctcgt catatcctaa accttgtttg attttatcag gatcgacatt gaagtactct
    42421 gccactgcag tagctgctaa agcattgtag acattataca taccgccaac ttcgattccg
    42481 tattcatgac catcgatcac gaaatctgct gacacattgt ccatccgtac catatcagtt
    42541 aatcgataat ccaattcagg acgtttgaag tcacaatttg gacagtaata tttccctaag
    42601 tttgcataag tgatcatttt ataatgcaag atgtgatgac aatttggaca taagacaccg
    42661 tctgtattgt aatgagccat ttgttcacga tcttcttcat ggtcaaagcc ataatatttt
    42721 ctaggattga ctgtttcgat agaattgaag attggtgaat caccgttact gataatcgtt
    42781 gcatttggtg actttgcggc accgtctaca atcattttat aggtcgtata gatttctcca
    42841 taccgatcca tttgatcacg gaaaatattt gtaaataaga acagttctgg tttcatatat
    42901 tctgtgactt tgctcaaact tgcttcatct atttctagga cagcaaactt tttctgtcct
    42961 ttttttggtt ttgcctgcaa gaaagtcgat acaattccct gtaccatatt cgcaccagta
    43021 gggttggtta gaacttcatc gaattgctgg cgcaaaatat tgactgttaa agcggtagtc
    43081 aatgttttac cgtttgttcc ggtgacaacg acgacttggt agtcttttgc caaactatct
    43141 agtatatgtg gatcgacttt cagcgctagt ttacctggta aacttgatcc gcctttaaaa
    43201 aaagttttta atgcccattg cgacgttttt ccaactgcaa tcgcaaaatg actgcgtatt
    43261 cccattatgt aacctccaaa aacgatttgc tgacgaataa attcaactaa tcataccata
    43321 tgttgaaaaa aaggttaaat tgcttttctt attcttaaga aaaggttacg gctgaaagtg
    43381 catgaaatcg gacaaaatcg catgatttaa caaaaaaaca gcttgaaaca aaatgattgt
    43441 ttcaagctgc tgtgatttta ttattccctc ttacctatat tcccatttta accgataata
    43501 attttctcgc ttggatattc atagcctaga tctcgtgttt cttttggcac gaataacatc
    43561 aatgtatata gcattccgat ccgcccaata aacatcagtg tagcaatcgt gattttgccc
    43621 acaacagaca agtctccggt aatccctaaa gataaaccag ttgttccaaa cgcagaagtt
    43681 acttcgatga tgatcgagat cagcgtttgt tcttctgtag cagataaaaa aacgatacaa
    43741 aagaaacaca taccaagaga aagcataaag acaacgactg attttcgcac atcatcttga
    43801 tcgattctcc gtccaaaaat attgatgtta tcttcacttt tgaggaagga atatagatac
    43861 aaaccgatga ttgctacagt agttgttcga atcccgccgc ctacagaact aggactgcat
    43921 ccgataaaca tcagtaaaga aaaaatgatc agtgttgtga tttgaaaatc acccaaatcg
    43981 tgaatctgca gaccagcatt acgagtcgta atcgaataaa acatcgaatt gatccatttg
    44041 acactcatat tggaatcttg gaataaatgg tccttttcca atagataaat caagacagtt
    44101 cccgaaacaa acaagataac aaaagctaac acagctaatt tggtaaacaa ggaaaaacga
    44161 aacggaagct tcgcattgga acgtttgtat aatagccatt cccggcactc catcaataca
    44221 ggaaaaccaa ttcctccgac aaaaatcaaa aacatgatca ggatcaagaa aaaatagtca
    44281 tgtgcaaagg gcttgatgga atccccagtg acgtcaaacc cagagttcgt gacagccgat
    44341 atcgcctgat aaaaaccgta aaaaacggca tcacgccaac gatcaaaata gcctctgtaa
    44401 taaaaataaa tagaaaagaa tgtcccaaaa agtaattgaa accagattaa aatagcaaaa
    44461 gtgatccgaa tcagtcggac gataccagac aaacgcggtt gattcatatc cgtcatgatc
    44521 agctgtcgct gtttcagtgt gatccgtcgt ttagaaaaaa tgacaaaagc agtagaaatc
    44581 atcatgatcc ccaaaccacc aacttgaaat aatatttcca gcagtataat accgttatca
    44641 ttgaacacag aattgatatc aaaagtcgat agtccagtca cactcactgt actgattgcc
    44701 atgaacagca tgtccacaaa agaaacatgg ctatctggtt cgcgaaaaat cggcatataa
    44761 aatagaacga gtgataagac cgtcatcaat atataataaa aaacgatgat ttggatcgat
    44821 gagaaatgat tggcagcaaa cttctgtcct cgccgctgca gccttcgaaa gaataaatca
    44881 acatgcacag tactatcctc ctgacaatag tcgtttctac cagtatacaa ggggaattag
    44941 gaatactcaa agaaattatc aaacagataa caatacagct ttcgattaaa atagaaatgg
    45001 cttataataa ggaaaatcct agaaaaaaga aggaggagga ataacaaatt gattaatgct
    45061 caacaaatcg tagaaaaaat gaaaaatcaa aaaataaatt atgatacagt tctgaaaaaa
    45121 atgatcatgc aatgggagaa agaagaagta aagcctaaga tattaataca tacatgctgt
    45181 gctccttgca gtacgcacac attggaattc atgtctcaat atgcagagat cacgttattt
    45241 ttttcaaatt cgaatattca tcctaaaagt gagtatttgc gaagattaca tgaacaaaaa
    45301 cgatttgtag accaatttaa tgagaaaact gaccatcatg ttgcgctaat cgttgatgaa
    45361 taccgtccaa atgaattcac caaaatggtt ttaacaaacc atcttgaaaa cgaaaaagaa
    45421 ggtggtgctc gctgcagtgc atgcttcaat atgcgattag atcttgttgc gcaaaaagcg
    45481 caagaactag gatacgatta ctttggaagt gccctgacca tctcgccaaa gaaaaatgca
    45541 gctttgatca atcagatcgg aatggatatc caaaagattt acgggacaca atacttgcca
    45601 agtgatttca agaaaaacaa aggctatgaa cgctctttgc aaatgtgcaa agagtatgat
    45661 atttatcgcc aatgttattg tggttgcgtg ttcgctgcca agcagcaagg atgcgatctg
    45721 aaagaaatca atcgggaagc aaaagcttac ttaaaaacat caaccgtagt ttttgaggaa
    45781 aattaaagaa atctttcgga aaaacgtaag aaaaaaataa agaatttatg gtagaaataa
    45841 ggtagactgt ataatttgtc cgtagtaaag taaggaaaca agaataaatt tcaatgaagg
    45901 aagagataag atgcgaaaag tagcacctat tttgaagcca atcgctgtat taacaatctc
    45961 gacgattgtc gcgactacta caggtattgt gaatagttcc agctaccgtg caccaagttc
    46021 aagttctagt actgtagaag aaacaacacc aactacttca gtctcgacat ctgaaccaac
    46081 aacaacggaa agtttgaatg aaaccactac gacaagtaca tgggacgata caacaactag
    46141 ttcgtcgtat gatgagacat atacaactga cagctcaagc gaaacaagta atacggtacc
    46201 agatggcact ccatcggatt caaccaatcc aagtacaagt ccggatacaa gtgctagctc
    46261 cgaaacgata ccaagcagta cgactgtaga tgataacacg caaaatgatg atgcagcacg
    46321 tacagcacag caaaaccaag attcaggaaa tacccaaacg tataaccagc aagcaggtga
    46381 ttctcaatga catttttact agaccttatc aacggggtgc ataaattcct tggctattta
    46441 gacatcagtc ctaaatatct taaccgtgga tatacgattt taagtgtgat tcccacgctt
    46501 tatttattga gaattgttta tggactttgg caaaaccaaa actatcttca atttttctta
    46561 tatggacttg catttctagt gcttgtttat tttactgttt taaatgtatt ttattacttt
    46621 ttagataaaa acacgaaagc cgatgtcact cagctttttg tgaagtattt acctgatgaa
    46681 gcgttcaaca tccagacaga agaatcgata gataatggaa attcgatcga taaagtgaat
    46741 acacaagagg tcatggtttc ttatgacgaa gattatcaat tgaaattagc cgagaatatg
    46801 cggtatttga ttggaaatgg tgagatcaaa acaaatgaac tcggtcgcat ggatggattt
    46861 ctggtcgata gaaatacatt gtatccttat tactatgtca agaaaacagg agagaaagag
    46921 tatgttctaa atattggacg aagctactcg gacatggaaa aagtagggac cattcgactt
    46981 gactcaacag aagacacatt ggaacctgtc ggattgttca tcgtcggcgg tgattttgtc
    47041 aaagacgggt tacgctatca tgagccttat cgtctgaaat tgattgcaaa aaaggcacag
    47101 tcgcaggaag aaccagtggt ttatagtcgt agccaaagaa gaagacattc ataatgtgat
    47161 gaaacgccgt gtatcagtgg ttgcgataaa aacgtttatt cggaataagg gggatgtgac
    47221 acgtcttttg tcacgttcct tttttatata acgagaaaga taagacgaac acatcactta
    47281 ttctttcctt tcatttataa acattgtaga ataaagaaag tacaacgaag tattgtgaaa
    47341 aaacgtaata aataacgagg aaaatacaag taggaggtaa gctgtgaaag aatttttgaa
    47401 aaattggggg attttgatcc ttgtattagc ggctatttta ttagctagag tgtacgtgtt
    47461 tacaccagtt actgtaaacg ggcattcgat ggaccctaca ttaagtgatg gacaacgatt
    47521 gatttcatct aagatctcaa actatgaacg aatggatatt attactacaa aggaaccggg
    47581 ggatgaagaa cggatgattg ttaaacggat catcggtatg cctggagata cagtcaagat
    47641 ggaaaatgac cagttgacca ttaatggcaa aaagtatgat gaaccttatc tggatgagtt
    47701 caaaaaagaa ttttcagaag ataagttaca aggagaatat gcttatagct caggattcca
    47761 agcacaagca gaaagttcat ccacctttac gagtgacttt gaatatactg tgccaaaagg
    47821 aaagtatctt gttctaggcg ataaccgctt gatttcaaaa gacagccgaa tgtttgggtt
    47881 agtcgacaaa gatatgatcc aaggaaaagt tgttttccgt tattggcctc tttcagaaat
    47941 taaaatcatg tgatgaaaaa ataaaaagaa gctgtgacat gttttttgtc acagcttctt
    48001 tttccgaaaa ttccttctta tttcttggag ttgctcactt tttttgcaat ctcattttaa
    48061 gctttttggc tgatggataa gaaaagcgaa gcgtttttgt tcataaatag cttttcccca
    48121 cgatggaagg tatgactagt agcagagaca aagcccgttt tttccaatcg attattcaat
    48181 tcttcttggc taaagccatt atggactttc ggatgactaa tttgttcatt cttgtcaaag
    48241 tctacgatga tcaattgacc gccaggagct aaaatttcat aaagttttgt aagaatattc
    48301 tccgtatcag gaatgtgtaa aagtacaagt gatagaagga tgagattagc ttggacagca
    48361 ggaaattcga cagaagcatc agcgtggatc gttcggacat ttttcagatc tgctgcttgg
    48421 atcttttctt ctgccatttt cagcatagtt tctgaagcat cggcaatgat cagttctttg
    48481 aatctttccg caagcggtaa actaacaagc ccagttccgc ctccgtaatc cagcaggact
    48541 ttttggtggc tgtcgcgcgg tagataagta gttagttctt cgcgaataat cttggctaat
    48601 gcttgacggt caggggaatc ataatggtta gcgatggaat caaaaatatt ttttgtcatc
    48661 aaatagctcc ttacgtaaaa ctttgaaatt ttaacttatt atagcacgtg aaaagaaaga
    48721 aagcatgcta tactcaattc aaaaagggag aatctagaaa gaatatgccg atatcgtttg
    48781 taaaagatag agaggaaaaa ggaaagtgtg tccgagaaat attactagac ttgcctgagt
    48841 ggtttgggct tcctgaatca acggaaaaat atatcgagga atcaagtaaa ctacctttat
    48901 ggtgtgaaaa aagaaaagaa gaatatttag gctttatcac tctttcacag acaagtgaag
    48961 atactgccga aatctattct attgtatggg aataaaaaaa gactatcatc accaaggatt
    49021 aggaacaaaa ctttttaaag aagcagaagg ttacgcagca aaacattaca aatatttgca
    49081 agtgaagact gtagacgagg ggcactactc tatatatgat caaactattt gtttctacga
    49141 atcattagga ttttcaaggt tggaagtttt tccaaattta tgggatgaat ggaacccatg
    49201 tttagttctt gtcaaaaaat tggaacaaaa gtgagcaata gatttaatac taatctgtgc
    49261 ttgatgaaaa ttctcactgt aaaaaatgct gaaaatcaag tataatacca taggaacaaa
    49321 ctggaaggtg aaaaagagca tggcacaact attttttaag tatggcgcaa tgaatagcgg
    49381 caagacgatc gagatcctaa aagtcgcaca taattatgaa gaacaaaata agccagttgt
    49441 cttaatgaca agcggtattg atacaagaga tggtttaggc gttgtttcaa gccgaatcgg
    49501 actgaaacgt aatgcgatgc cgatatttga agaaacagac gtatttgacg taattcagaa
    49561 aatggaaagc aaaccctatt gtgtgctgat cgatgaagcg caattcttaa aaaagaaaca
    49621 tgtcattgct tttacgagaa ttgtggacga attggatatt ccggttatgg catttggttt
    49681 gaaaaatgat tttcgaaacg agttatttga aggatcaaaa tatttattgc tttatgcaga
    49741 caaaatcgaa gaaatgaaaa caatctgttg gttctgccac aaaaaagcaa tcatgaattt
    49801 acattatatt gatggaaacc ctgtttatga aggcgatcaa gtgcaaattg gcggaaatga
    49861 agcttattac cccgtttgtc gaaaacatta ctttcaccca tcaatgatta cagaaggaga
    49921 ataagcatgt acgatcaatt acaagcaatt gaagaccgtt atgaagagct tggagaactt
    49981 ttaagtgacc cagaagtgat ttctgataca aagcgtttta tgcagctttc aaaagaagaa
    50041 gcaaacacgc gcgaaacagt cgaagtttat cgtcgttata aagaagtagt acaaggaatc
    50101 accgatacag aagaactgct tggtgaaaaa ctggatgatg aaatggctga actagcaaaa
    50161 gaagaattat ctgaattaaa aaaagaaaaa gaagtcttag aagaaaaaat caagatcttg
    50221 cttttaccaa aagatccaaa tgacgataaa aatatcatca tggaaatccg tggagcagca
    50281 ggcggagacg aagcagcatt attcgctggc gatcttttca atatgtacca aaagtatgct
    50341 gaagcacaag gctggaaaac agaagtgatg gaagctaacg ttaccggaat cggtggatat
    50401 aaagaagtga tcatgatgat caccggagac aacgtatatt cgaaattaaa atatgaaagt
    50461 ggtgcgcacc gcgtccagcg tgttccttca actgaatcgc aagggcgtat ccatacttcc
    50521 accgcaacag ttgtcgtgat gccagaagct gaagaagtag agatcgatat tgaagacaaa
    50581 gatatccgta cagatatcta tcatgcttca ggtgctggcg gacagcacgt caataaaaca
    50641 gcatctgctg tacgtttgac tcacttacca acagggatcg ttgttgctat gcaagatgaa
    50701 cgttctcaaa tcaagaaccg tgaaaaagca atgaaaatct tgcgtgctcg tgtgtacgat
    50761 aagatccagc aagaagcaca aagcgagtat gatgccaatc gaaaatcagc agtcggaact
    50821 ggggatcgtt cagaacggat ccggacgtat aatttcccgc aaaatcgtgt gactgatcat
    50881 cgtatcggat taactatcca aaaattagat cagattttag cagggaaatt agatgagatc
    50941 gttgatgcat tgatcatgta tgatcaaaca tcaaaactgg aagctatgca aaatgggtaa
    51001 aatgacctat cgagaagtcc tgtcacgggc ttcttctttt ttagaggatc atgggaaaga
    51061 agcatatagt atccaatttt tatttttgga acgaaaacaa tggaaaaaac ttgattggct
    51121 ccttcatatg aacgaagaga tttcagaaga agaacagcgg ttgatcgaaa ccgatctgcg
    51181 acttctgctt gcagatcatc cacctcagta tttgctagga tatgcagatt tttatgatca
    51241 tcggttaaag gtaactgaag caacactgat ccctcgacca gaaacagagg agttagtcga
    51301 atggtgttta gacgagacgc ctggtgtacc gttagaagtg atagatattg ggacagggac
    51361 tggagcaatt gctattagtt tgaaagcagc aagaaaaaat tggcatatat ctgctgtgga
    51421 tctttcagaa gaagcattag aagtagcgaa agaaaatgct cagaaagaag gaacgaaaat
    51481 cagtttttat catggagata cattaggacc agtaatggat cagcaatttg atgtgattat
    51541 ttctaatccg ccgtacatca gtcggaatga atgggaactg atggatgaaa gtgtccggtc
    51601 attcgaacct aaaatggccc tttttgctga gaacgatgga ttagctattt atgaaaaaat
    51661 agcagttgaa gcaccaagcg tactaaaatc aaatggaaag atttttttag aaatcggatt
    51721 tcgccaaggt gaagcagtaa aaaaaatatt tcagcaagca tttccagata aaaaagtggc
    51781 gataaaaaag gatctttttg gcaatgaacg aatgatccaa gtttatggtt gaagttgtgg
    51841 atgaaaaatt ttttatttgt ttttttatta taaaaaaaca cttatttgac aaggatgtaa
    51901 acgacttttt ggtttatcaa caagtttatc cactattgac aaatagatta tccacaaaca
    51961 gtggaaaact tgaggataaa ctcagacaaa ataagaatgt atgaaaaagg ggttgtggat
    52021 aaactgtgga aacgaaacga tatgatgaaa cagaattgca agaggcagcc gaagcactga
    52081 aagaaggaga acttgttgct tttcctaccg aaacagtata cggattgggc gccaacgctc
    52141 ttttaccaaa cgctgtcaag aaagtgtttt ctgtaaaagg gagaccgcaa gataatccat
    52201 taatcgtcca tgtcgcctct tttgaacaag taaaagagta tgtggacaac tttcatccgg
    52261 cagcagagaa gattgtgaag aacttctggc ctggaccatt aactttgata tttaagatca
    52321 aaaaagacac tttgccctca gttgtgacgg gaggattatc cacagctgca tttcgtatac
    52381 cggataacaa aaaaacacta gaagtaatcg aactttccgg tgttccgctt gtcggtccaa
    52441 gtgcaaatac gtcgggaaaa ccaagtccaa cgactgcaga tcatgtatat catgatctac
    52501 aaggaaaaat taccgggatt attgatgatg gagctacgcg aattggtgta gaatccactg
    52561 ttcttgattt aagtgatcca acagccatgc ctatgatttt acgaccaggt gcagtaacga
    52621 aagaacaaat cgaagcagtc atcgaatctc ctgttgctat tgatcagcat cttgtaaaag
    52681 aaaatgaaac accaaaagcc cctgggatga aatacaaaca ctactcaccc gatacacgtg
    52741 ttctgatggt cagagatggc gattggtcaa ctgcagttca atgggcaaaa aataagaaga
    52801 tccgtgcagg tgtcatagca agccccgaaa ttgcggatca agtgcggaca gatactgcag
    52861 ctgtttatat gtataacgac aactcagtag aagcggcggc taaaggattg tttgcaggtc
    52921 taagaggatt agatgaacca acacttggat tagatttgat ttttgtacaa gtgtatccag
    52981 aaactggatt ggggaatgct tacatgaatc gcttgaaaaa agcagcagga caaaattatt
    53041 ttgaaaaatg atcaattggt tattgtcgca ttcttttagt atgatacaat gctatcaagt
    53101 aaatcttcta tcagtgaacg aaaaactaag cgtaactaat cgtaatagaa gagatacgaa
    53161 tatttggcaa tttgtttttg atgggaaaaa taaaattgta ggaaaataaa attgtaggaa
    53221 aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga tctttgggct
    53281 gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc ctctgaaaac
    53341 tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa atacgcagaa
    53401 ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt tgaaaattta
    53461 gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca gcctcactcc
    53521 ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga cacgatctta
    53581 ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa cttcagtggc
    53641 aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat tgattataat
    53701 gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg tgccagtgct
    53761 tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt aggcgcaaaa
    53821 ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca tcctaatcca
    53881 gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg ccctcgaggc
    53941 ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc ggtatttcct
    54001 ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc attcaaagaa
    54061 gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc aaaagcaatg
    54121 gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac agataatcat
    54181 ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga gagcattttg
    54241 gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact aagtccattc
    54301 aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt caaagaagaa
    54361 gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga tgataacgct
    54421 caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc gttgcataaa
    54481 aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct tttctgttta
    54541 ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat atgaggtgta
    54601 attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt atctcgttag
    54661 aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc tatgaactag
    54721 caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg aacaaatatt
    54781 ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc aatattcgtc
    54841 tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt catggagcag
    54901 gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt gcggttcgta
    54961 cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa tatccaacag
    55021 caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag tcaaaaaatc
    55081 tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg gctacagtca
    55141 tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg ctgtattatc
    55201 cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa aactattttt
    55261 tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa gaagagcgtg
    55321 ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat cttccttcag
    55381 caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct tatgcgcgca
    55441 aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc atccatgatt
    55501 ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg gcattatcta
    55561 cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact tcttttcaaa
    55621 aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat tttgttttac
    55681 aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt tcaagtgatc
    55741 gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg cggaacaaag
    55801 gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt atcacgggat
    55861 atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca aaaaacgttg
    55921 tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat ggttgacggt
    55981 atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg agatgaagaa
    56041 acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc acgtcagttg
    56101 tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat cgatgcattg
    56161 aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc tgagggagta
    56221 aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct agatgaaaaa
    56281 ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg tttgtttggt
    56341 acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc ttttttggtc
    56401 atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata taaaaaaacc
    56461 atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt agttaacgat
    56521 tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat caatctcaac
    56581 aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc cgtgaattgt
    56641 gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta cgatagttgt
    56701 atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca attataccac
    56761 gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt acaatcagcc
    56821 aaatagagta tataatttct tttggataag gccagcttag ctcagttggt agagcaacgc
    56881 actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag agaagacaga
    56941 tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg aaataaagta
    57001 tgctttctta gcaatagagc atgtattatt tctataa
//
LOCUS       DASJSZ010000007        55507 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.007, whole genome shotgun sequence.
ACCESSION   DASJSZ010000007 DASJSZ010000000
VERSION     DASJSZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.007"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            3..371
                     /locus_tag="U4B03_000397"
     CDS             3..371
                     /locus_tag="U4B03_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEN2067275.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="U4B03_000398"
     CDS             551..1492
                     /locus_tag="U4B03_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEN2067276.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="U4B03_000399"
     CDS             1561..2292
                     /locus_tag="U4B03_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEN2067277.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="U4B03_000400"
     CDS             2436..4616
                     /locus_tag="U4B03_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEN2067278.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="U4B03_000401"
     CDS             4636..5118
                     /locus_tag="U4B03_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEN2067279.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="U4B03_000402"
     CDS             5134..5895
                     /locus_tag="U4B03_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN2067280.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /locus_tag="U4B03_000403"
     CDS             6026..7360
                     /locus_tag="U4B03_000403"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEN2067281.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="U4B03_000404"
     CDS             7667..8152
                     /locus_tag="U4B03_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEN2067282.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="U4B03_000405"
     CDS             8145..8828
                     /locus_tag="U4B03_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEN2067283.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="U4B03_000406"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="U4B03_000406"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEN2067284.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="U4B03_000407"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="U4B03_000407"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEN2067285.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /locus_tag="U4B03_000408"
     CDS             12444..13073
                     /locus_tag="U4B03_000408"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN2067286.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="U4B03_000409"
     CDS             13125..13457
                     /locus_tag="U4B03_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN2067287.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="U4B03_000410"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="U4B03_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEN2067288.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="U4B03_000411"
     CDS             14283..15656
                     /locus_tag="U4B03_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN2067289.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="U4B03_000412"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="U4B03_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEN2067290.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="U4B03_000413"
     CDS             16711..17151
                     /locus_tag="U4B03_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN2067291.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="U4B03_000414"
     CDS             17295..17996
                     /locus_tag="U4B03_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEN2067292.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="U4B03_000415"
     CDS             17993..19114
                     /locus_tag="U4B03_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEN2067293.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="U4B03_000416"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="U4B03_000416"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEN2067294.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="U4B03_000417"
     CDS             20493..21401
                     /locus_tag="U4B03_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEN2067295.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="U4B03_000418"
     CDS             21436..21708
                     /locus_tag="U4B03_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEN2067296.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="U4B03_000419"
     CDS             21719..22765
                     /locus_tag="U4B03_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEN2067297.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23016..25625
                     /gene="clpB"
                     /locus_tag="U4B03_000420"
     CDS             23016..25625
                     /gene="clpB"
                     /locus_tag="U4B03_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEN2067298.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25776..26462)
                     /locus_tag="U4B03_000421"
     CDS             complement(25776..26462)
                     /locus_tag="U4B03_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067299.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26440..26934)
                     /locus_tag="U4B03_000422"
     CDS             complement(26440..26934)
                     /locus_tag="U4B03_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN2067300.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26954..28174)
                     /locus_tag="U4B03_000423"
     CDS             complement(26954..28174)
                     /locus_tag="U4B03_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2067301.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28321..30156
                     /gene="lepA"
                     /locus_tag="U4B03_000424"
     CDS             28321..30156
                     /gene="lepA"
                     /locus_tag="U4B03_000424"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEN2067302.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30352..31263
                     /locus_tag="U4B03_000425"
     CDS             30352..31263
                     /locus_tag="U4B03_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2067303.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            32259..32510
                     /locus_tag="U4B03_000426"
     CDS             32259..32510
                     /locus_tag="U4B03_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067304.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            32593..32865
                     /locus_tag="U4B03_000427"
     CDS             32593..32865
                     /locus_tag="U4B03_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067305.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            32955..33194
                     /locus_tag="U4B03_000428"
     CDS             32955..33194
                     /locus_tag="U4B03_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067306.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            33341..37624
                     /locus_tag="U4B03_000429"
     CDS             33341..37624
                     /locus_tag="U4B03_000429"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HEN2067307.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            37723..38088
                     /locus_tag="U4B03_000430"
     CDS             37723..38088
                     /locus_tag="U4B03_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067308.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            38090..38542
                     /locus_tag="U4B03_000431"
     CDS             38090..38542
                     /locus_tag="U4B03_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067309.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            39156..40466
                     /locus_tag="U4B03_000432"
     CDS             39156..40466
                     /locus_tag="U4B03_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN2067310.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            40489..40800
                     /locus_tag="U4B03_000433"
     CDS             40489..40800
                     /locus_tag="U4B03_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067311.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            41160..42416
                     /locus_tag="U4B03_000434"
     CDS             41160..42416
                     /locus_tag="U4B03_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN2067312.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            42413..42958
                     /locus_tag="U4B03_000435"
     CDS             42413..42958
                     /locus_tag="U4B03_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067313.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            42959..43459
                     /locus_tag="U4B03_000436"
     CDS             42959..43459
                     /locus_tag="U4B03_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067314.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            43540..44096
                     /locus_tag="U4B03_000437"
                     /pseudo
     CDS             43540..44096
                     /locus_tag="U4B03_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            44116..44460
                     /locus_tag="U4B03_000438"
     CDS             44116..44460
                     /locus_tag="U4B03_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067315.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            44479..45390
                     /locus_tag="U4B03_000439"
     CDS             44479..45390
                     /locus_tag="U4B03_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2067316.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            45469..46461
                     /locus_tag="U4B03_000440"
     CDS             45469..46461
                     /locus_tag="U4B03_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2067317.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            46458..46676
                     /locus_tag="U4B03_000441"
     CDS             46458..46676
                     /locus_tag="U4B03_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067318.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            46689..47099
                     /locus_tag="U4B03_000442"
     CDS             46689..47099
                     /locus_tag="U4B03_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067319.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            47151..49679
                     /locus_tag="U4B03_000443"
     CDS             47151..49679
                     /locus_tag="U4B03_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2067320.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            49695..51905
                     /locus_tag="U4B03_000444"
     CDS             49695..51905
                     /locus_tag="U4B03_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067321.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            51913..53004
                     /locus_tag="U4B03_000445"
     CDS             51913..53004
                     /locus_tag="U4B03_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN2067322.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            53025..53567
                     /locus_tag="U4B03_000446"
     CDS             53025..53567
                     /locus_tag="U4B03_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067323.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            53762..54019
                     /locus_tag="U4B03_000447"
     CDS             53762..54019
                     /locus_tag="U4B03_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067324.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            54033..54365
                     /locus_tag="U4B03_000448"
     CDS             54033..54365
                     /locus_tag="U4B03_000448"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067325.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            54410..54958
                     /locus_tag="U4B03_000449"
     CDS             54410..54958
                     /locus_tag="U4B03_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN2067326.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            54965..55417
                     /locus_tag="U4B03_000450"
     CDS             54965..55417
                     /locus_tag="U4B03_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067327.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
BASE COUNT        18859 a         9012 c        11944 g        15692 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 cattaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    22981 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23041 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23101 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23161 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23221 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23281 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23341 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23401 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23461 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23521 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23581 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    23641 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    23701 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    23761 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    23821 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    23881 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    23941 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24001 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24061 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24121 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24181 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24241 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24301 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24361 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24421 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24481 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24541 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24601 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    24661 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    24721 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    24781 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    24841 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    24901 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    24961 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25021 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25081 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25141 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25201 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25261 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25321 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25381 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25441 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25501 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25561 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25621 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    25681 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    25741 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    25801 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    25861 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    25921 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    25981 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26041 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26101 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26161 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26221 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26281 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26341 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26401 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26461 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26521 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26581 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    26641 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    26701 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    26761 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    26821 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    26881 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    26941 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27001 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27061 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27121 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27181 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27241 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27301 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27361 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27421 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27481 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27541 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27601 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    27661 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    27721 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    27781 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    27841 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    27901 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    27961 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28021 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28081 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28141 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28201 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28261 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28321 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28381 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28441 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28501 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28561 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28621 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    28681 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    28741 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    28801 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    28861 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    28921 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    28981 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29041 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29101 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29161 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29221 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29281 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29341 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29401 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29461 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29521 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29581 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    29641 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    29701 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    29761 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    29821 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    29881 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    29941 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30001 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30061 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30121 gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat
    30181 taaataaacc tttttgagta gatgcaacac tgaggattat ctgtataaaa gattttaaac
    30241 tgtcatttgg ttatcacggc gatactaaaa ttaacaaaat tggaatattt ttcttgaaga
    30301 tttttagctg tcagtatatg atgaaagcat ctcataggag gtgctttttt catggttgat
    30361 aattcaagtg gaagggttct taaatctcta agaaaagaga aaaaactatc tcaaaaaaag
    30421 ttagctgatt tagcaggtat ttcacaaagt actttagtta aatatgaaaa agggtcaaga
    30481 aaaatcccta aagacgtaga taatactcta agcaagattc tgaatataga aactctaata
    30541 aaagatgagg aagataagat tgagattctc atcggtaaac taattgcata cagagatatg
    30601 aataagttac ttaacaaaga gttagctgat aatataggaa ttagtgaagt attactcagt
    30661 tatgtgttaa atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat cgatattttt
    30721 ctattaagta atgaaaaaga aattttgaag gagattaatc gtgatagtga gatatttagt
    30781 ttatcaaaag acgataaaat tgttatggga aaaagaatac gagaagtccg aaagaaccgg
    30841 gaagagacgc ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa aaatgttata
    30901 agtagatggg aaaaaggtat taatattcca gatattgaaa aactaatgaa tattgcctat
    30961 cttggtaagg tcactgttcc ttaccttatg tacggagaag attataagaa tatacttcca
    31021 aaagatgaaa gagttagcga ttttaaaaag ataaattctt ttagtatggg attaaggatg
    31081 aggaaaatta ggaaagatta ttatctagaa agggaggagt ttggaaagct tttttctcct
    31141 tcaatttcaa aatggtccat agataggtat gagaatggta gagatatacc caatactaat
    31201 cgaattatcc aatatgctta tataggaaat ctttctttag aatttttgat ttacgggata
    31261 taaaaatagt ttacgtcgat acttattaag ttatcgacgt tttttattat agaaaaaatt
    31321 tataaaagga ggtttcttga tatgataaga aacaaaaagg gtttgttgaa agttgcttta
    31381 ctggcagctt ttttctaagt aactttttga caagctcaat ggcaacacaa gcggaagaag
    31441 taatacaaga actaactgtt atgaataaca gctagataaa atgggctatg tatgttatcc
    31501 aacaatttag taatcactag aagcctagcg aaagttcgct tgataagtta tagatgagta
    31561 ttgatttgta gtgtttccaa atcaaaaggg tacagagcca catgaaatat gttgtcctct
    31621 gcagcgtcag gatgggaatc cataccatgg agattcggag atctgtgagg gaatacttac
    31681 gtggttaata tgtcagttgt gttggacgta gtaatggtca tagtgggtag attgactgaa
    31741 ttcttatatg agaaaatgaa accaacaaga agatagggtt atttatacag aattggggat
    31801 cttttctgga aaagtaatcg gataatgcga gattcgtacg gtctttttta aatttctcta
    31861 aaaaaaacgg catcgaatgg gttgggtgat tgataagcaa tgaatcatag actgatatag
    31921 aataaaaaat gattctattt atctttttac atctatcgtt ttttcaagta gccgcaaatt
    31981 atatcagcat tcaacaattt ttgtatacaa gttctagtaa aaggatctga agaaaaagtt
    32041 ggttgttgtg aagataaaaa aagaatcagg ttttactgag tttttcttca aaatttgtgt
    32101 tattaggtta ggacaaagat aagaagaagt atgaagattt aaaaaaggaa tgaggaaaag
    32161 atagtaattt ccgattattg gagttttttt atttataaag aagaagcgaa taagttgtga
    32221 ttcctagatt tcgcgcgtga ttaattaatg agcaaaaaat gcggaaagtt ttagtgttta
    32281 atacacgagg acaaagtttc ttaggtattg aaggcacacg tgtaacggtt gatgggtggt
    32341 gtgattgttt cgaggttgga aaacatgttg aacttatggc atttactgag ctaaaagata
    32401 agaacaacgt ggaatttttc gagagtgata ttggttggga cgatcatgaa gaagtttatg
    32461 gaaaatttat tttgcggaag ggaaattctt atacgaatgg ggaagcataa gtagcgattt
    32521 gttagagatc attgatagga ttgaaattgt cgggaatatt tatgagaatc tagagttatt
    32581 tgaggtgata gaatgagtga tggttttgaa caagtatcaa taattaaaaa tcaagttcgg
    32641 gagattctta gaaaaaaagg ctatttggta gatagttatt ttgaaggaga ttacgaaaca
    32701 tgggtaggtg tctatgctcg tccagagaat aaacctacct atttagatcc tacgacgtca
    32761 gaagatgcct atttacaaaa taggtatcga gtagatggtt ttaagcaaga ctttgcggag
    32821 tggtttgaat gggaaattga aaacggagaa gtaaaagaag aataagagta cttgttgtat
    32881 caaaataagc cagtagaaca accaaaccag tagctagtaa tgacaataat actgaggaat
    32941 cggatcatta tggtatgatt gatatttctc ttccagaaga tcttaagcca gatcctgtaa
    33001 caattggcaa tagtgtcatg atttttgatt cattagatga agctatgtca tatggttgga
    33061 aagaaattaa ggatgaaggt agtaattggt atggcatgag atttggtgat ggtgaagttg
    33121 ccaaaaaagc tggtattcca attgggaaat ggacagttga tttccacgaa gcaggttctt
    33181 tttttgttct gtaattatag tgggagggta aaatcattga tgaaatgtgt tcagataaga
    33241 gcgccagata aaaatagaag agacagttca cgtcgttatc acttttggta acggcgtttt
    33301 gttttattat ataaaattct caggaaaagg aggaattctt gtgtatgcaa aaacgaaaat
    33361 gaattggttg aaagcactcg cattggtggc tttttctcaa ttctgtttct tggtgggaac
    33421 tgctcaagca catgcagaag aaacgacggc tatacaacct acgatggaag ccattgccca
    33481 agtcgcttct gaagtgacag aagtccaacc agcagaagcc ggggtcccag ctggtgaagc
    33541 cactgttgaa caggcccctg tgacagaagc gccaacggtc aattccaaaa cgactctcga
    33601 tcagacgatt ggggaaacac aacacccagg gaatagtcct ggtacttcac aaccagttga
    33661 agaaactgcc tcaattacaa cccctgcttc agaaacgtcc actctagaag gagaaaaagc
    33721 tgccgaagca acgccttctg tgcagccaga gtcttctgag aaacaagcgg aagaaaagaa
    33781 acgaatagaa gaaacagtgg aacaaccagc ggctactgct gacaaagaag gaaaaaatga
    33841 ggaaaacgtt gaaaaaacaa tctcttcaac cgcagctagt caaaatcaac caacgaaacc
    33901 aaacaaagaa tctacggcta gtattgaaaa tcaaaagaaa aaagagcaga acattccaaa
    33961 aacaggtaca cctacactgt tagtaacagt accaaccaat cgttcggttc gtgttcgccg
    34021 tgcaaccaat gcagctgttt taggtggtcg gaaaattgat tggggcgaaa atattggtgt
    34081 tacttctagt ccgaacaatc taaacttcac tttaaattat atggttcgtt ttgagattaa
    34141 tggacaagtg gcgttttgta ttgagcctgg tgtctcagcc gcagaaggtc aagtttatac
    34201 agctactgct ttgaaaaact atttgaaaaa ttcaaatgta cggaaaaaaa caagcttgat
    34261 ctcttattta ggatatgtca caaataaaga caaatccgat gaacaataca ttgcggcaca
    34321 atttatgatt tgggaagctt tgggcacaaa aatcccaatc aaaaatatcc gtgttgatta
    34381 tgatcgtcga aaagcagaaa ttcaacgttt gattaataac tttggtgaaa aagcaacttt
    34441 taataatcaa acacatacgg taaaaattgg cgaaacgttg aaagtaaaga ataaaaatga
    34501 ttttttgaaa tatgtgaaag acattcaaaa accaaaaggt gtcaatgtat ctattcacgg
    34561 caatgaagta tggattacag tcacaaaaga ggcaccggat aaagcaaaaa ttcaattgaa
    34621 taaaattaaa gttcaaggtg cgccattagc ctatcgtcgt ccaggagctc aaacaattgg
    34681 tgtgttacat ccaacagaac ctaatgcgtc tgttattaat ttacaaattc taaaacaagg
    34741 aaacattcgt gtgtttaaag aagatgcgga aactggtgtg aaagcacaag gtgcagcttc
    34801 tttagaaaat gcggtctatg gtttgtatca agcagatggg aaaaagatta aagaaattac
    34861 cttgaaaaaa attaacggca aagtacaggc agaaattaaa gatcttgatc ctggcaaata
    34921 tgttttaaaa gaaatcaaag cgccaaaagg ttatgtgctt tctgatgaag caattgaaat
    34981 tgttgttgaa cctggaaaaa ctgtcaatgc gaccgcaaaa gatcaagtga tcaaaggaac
    35041 cattgattta gtgaaagttg ctaataaaga tttagtcgat tcaacgaatc cagataacaa
    35101 accaaaatta gctggcattg aaatcacttt gacgtcaaaa acaactggca aagtggtgaa
    35161 aaccgttgtg acagacaatg atggttatgc tacttttggt aaaaataccg ttgtctttga
    35221 tacgtacatt ctttctgaaa ccaaaggaaa agaagggtat aaactatttg aaccacttga
    35281 agtcacaatt tctgaaaatg ggcaaacttt ccattatgtt ttagaagata aagtaattga
    35341 acaacgtctt aaagtggtga aagtggatca agaaacagga aaagtgattc cacttgctaa
    35401 tacagaattc aagatttttg atactttagc caatcgttat gtcacaatgg agattccaaa
    35461 tgatacagaa acaacggata tctttaaaac caatgaaaaa ggctttttcc taactaacgg
    35521 tacacttact tatggaaaag atcgctatcg tttggaagaa atcaaagccc ccgtaaatta
    35581 cgttttgaat caaaacccat tagtgttctc gattaccaat gatacagaac cgatcaaagt
    35641 gatccatttt gcgaaccgtc aagcacggaa aagtgtaaaa ttattcaagt atgaagaatg
    35701 gaacaagaag aaaacgccgc tcgcaggtgt gaactttacc ttgtataacg gtaaaaatca
    35761 agcaattggt gaatatacca ctgatcaaaa cggggaaatt ttagtgaaag gtctagttgc
    35821 cgatgactat tacttcttgg aaaatgcccc attagaaagt ttccaaccaa atacagataa
    35881 acaagcattc acggttgcat tcaaacaaaa tgcgccagat gatggggaaa cgttagtggt
    35941 ttctgtacgt aattacttga ttccaccaaa actagaaaca caagcgtcaa ataaagccga
    36001 tggtgaaaaa gtggttgatc cattagaaac gatcgtcatt aaagacagtg tttcttatac
    36061 caacttgttt gtcggaaaag aatacaccat ttcaggagta ttgatgaaca aggcaacgaa
    36121 tgcgccaatt ttacaagatg gcaaagaaat cacaagtacc ttggccttta ttgcagacaa
    36181 agtcaatggc acaaaagacc ttgaatttgt ggttaatgct agtgttctga aaggtcaaac
    36241 gatcgttgtt tttgaaaaac ttttccgtga tggcattgaa gttgcagcac atgcggatat
    36301 caacgatgaa gcccaaagtg tgaaagtgaa taatccaacc gttcatacca cggctgtctt
    36361 tgaagacgga ttgacagtcg cagacccaac aggattgttt accttaaacg ataccgttga
    36421 aattaccgag attcttccag gaaaagcata cttggtcaaa gggatcttaa tggataaagc
    36481 aaccggtgaa cccttgcttg tgcatggaaa tacggtagaa acgagtgttg gctttattgc
    36541 caaagcagaa aatgaaacgg tgatcgtacc atttaccttt gatttgacag gtataaatgg
    36601 tcgtgaaatc gtcgtgtttg aaagtctttt tagaaatgat gaagaactag caaaccataa
    36661 agacatcaac gacaaagggc aaacggtacg agtaactaac ccaagtgtac gtacaacggc
    36721 tacacataaa cccactggct tgaaagaggt taatccatta acgaaagtca ccattacaga
    36781 cgctgtgaaa taccacgatt tggtcgttgg aaaaacatac acagtcaacg gtgttctaat
    36841 ggacaaagta acggaaaaac cagtgctagt agatggaaaa gaagtcacaa gtaccttaac
    36901 gtttgtggca gaaacaacgg atggtacagt caatctagac tttattctag acgcacgtgc
    36961 gttgcgaggc aaagaagtcg tggtctttga agatctgttt agagaaggcg tattattggc
    37021 ttctcacgca gatattaccg acaatgacca aaccatcaaa atcagtaatc caaccattac
    37081 gacgaaagcg acgaataaaa atggcggaaa agagatcttg gccttgccaa atcaaactgt
    37141 gcttgaatgg gtcaaagtag acggtcttgc gattggtcaa atctatcgct taattgttca
    37201 aggctactta acaccagacg gcacaccatt tgaaggcact catgcggaaa aagtctttgc
    37261 ggccgataaa gaaatgatgg aattcttatt tgaattctta gtggacggtc gcaatcttgc
    37321 aggaaaaggc ttgagttttg cggaatggct ccaagcgaaa acggaagatg agaaagaaac
    37381 gtttgaagaa gtggcgaaac acaatgtaga tctaacaaac aaagatcaaa cggttcaaat
    37441 tgtggaagcg ccaaaaccac cgcaaccagt gaaaccagaa caacgggtta aaccagctac
    37501 tgttgtacca atgttgccaa aaacaggtga acaaacaagt agtatactta tggttcttgg
    37561 attagtagta ttagtcattg ctttaggtat aacactttat aaagtacgta aagccaaaga
    37621 ttagtcatga ggaacaagcg gttaggattt ttaaccgctt gtttgattgt aatgtagaaa
    37681 tatttagaat ttattttggt ttttaagagg aggaaaaact ttatggcaga aaaagcattg
    37741 cgtatgaaga aagagttttt aggtcaagta tttttattgg gttcacggga acctaatcca
    37801 ccaaaggaaa ttaatggtgt gtctgggcga tatgttcaat tacaagcaga gtgttcgcga
    37861 gaagaacagc ctgctttttt tgtttatctc acagctgaag aggataaaag attacgatta
    37921 ccagaaaaag aggtgcgtat ttcatttgaa gaagcttatt ttgtttttcg taaagaacga
    37981 gtgaatagtc gtgaaacaaa tgatatggga caaacagagc gtcgaacaac tggctatcaa
    38041 gatgttggtg tctttttaac tggtgtaaaa attggggagg ttttgtaaga tgggattaaa
    38101 atttccaaat aattatgaca ttacagaagc attagatgaa acttgtttag gagccgtacg
    38161 ctttggcgca ttagaagagt ctgatcgtgt atttgataat gaaacaggcg attatacggg
    38221 agaaattcga tcacagcgag cacaattagt ttttggaaat ccagatgaac caaagattgc
    38281 acgacgagtt aaatttccag cagatgtaga tttgactggt tttaaacgac gtgatcctgt
    38341 gaaattaatt gatccagaat atcatgtggt atatattcca gcacaagatg gcaatcgtga
    38401 atattcagat ttagtgattt atgcgaaagg attagagaaa gtagaagctc caacaaataa
    38461 tcctaatatg aaacaatcac aagaaaaagg tttagatcaa aaacaaccaa gtgttgattc
    38521 taaggaaaaa gagaaaaaat aaggtttttt tgaaagaata ggaaggagtc ttcctattct
    38581 ttttatctaa gatagaagga aggggagatt ttgaattcag ataaaaccaa tcaaatactt
    38641 tgagagattg tgcttgttat caaaatatcc aacaattgat tgagtatttt ggagacaatc
    38701 caacgatttt tgtacaaaca aatgcatata tttgtgcggt tattgggcag tgtatgtttt
    38761 tacgtgatct tgttcgtttt tcattaccgt accagtggaa acaagatcat caggaagtga
    38821 tttggattcg tatagtacga atatgtaata tgtgtgcaca cccttataaa aatcgattaa
    38881 atctttttcg attatgtttt tttactaaat gtgatattcc tgtattagtt gctttttgtc
    38941 aaaagttttt agcacaggaa aagtaaaaac agaaaggagg aatgtgacaa ttgaaagctc
    39001 attttttcaa gtatcgtggt cggcgaattc gctatttcaa tcgttattta tggttttgct
    39061 attatggttt ctttttgttt ccatttttga ttctgttagg ttggtttagt tggaaagtat
    39121 tttatccacg tttaagtacg ttttcgaatt ggcaaatgta tcttattcct ggaataagta
    39181 ttgtgttgta tttgttgctg gttagtgggt taattttagg gttgatgcat tggtcgcgac
    39241 taaaagaagg atattttgcc tctgtttatt ggcgtcagtt acttgttcga atgttaattg
    39301 acaatcggtt tgtttataca aagaaacaag ttagtgagaa acaaacaagg gaaaaaatga
    39361 gattaccaaa agtttatttt tatcaaaaga aacaagaatt agtggtttct tttcctttag
    39421 atggtgggcg ttttcacgaa cgatttttaa aaatgggtca tttgttatct gaagtatttt
    39481 tgcgtgattt aattcgtgaa gaacgagaaa aagcccgaat caattatgta ttcctttcag
    39541 attctggtcg gattcctatt gatcaatgta tagcagaaaa tggacgtatt catttaatga
    39601 aaactttgga ttgggtatat gatcaatcac caaatatgtt gattgctggt gcaatcggtg
    39661 gtgggaaaac ctatttattg tattctttaa ttcaagcttg tttaagtgtt gggacagtag
    39721 atatttgcga tggaaaagcg gcagatttag cagcattagg tgacgtcgat gtattcaaag
    39781 gacatgtgtt ttacaaaata aatgaagaaa tgattatttg tttacgaaat gcattaaaag
    39841 aaatgaatca acggtatgcc tatatgaaaa ccatgaaaga tcctaaatat gaaccaggga
    39901 aaaattatgc ttattatggg ttaccaccgc attttatctt ttttgatgaa tgggcagctt
    39961 tttatggttc gttggatatg gaagcgagaa atagagtcga taaattgatt cagccattag
    40021 tattaaaggg tcgtcaagca gggatgtaca ttattttagc aatgcaacga ccagatgcag
    40081 aatattttcc aagtggtgtt cgggataatt taacattccg tatttctgtt ggtcgattgt
    40141 ctcccattgg ttatttaatg acttttggag aagattttaa aaataagcca ttttacaaca
    40201 aagatcaacc tggtcgtgga tatgcttttc caggatttga tgtacctgta gagttttttg
    40261 cgccatttgt ttacaaagat tatcacttta tcaaagaatt tgagaaattt actgaaatga
    40321 ttgtctatga ttttacagta aaagaggata gtcaagatca aacgatggga gatgataatc
    40381 agttgttaca agaacaacta gagcgccaaa tgtatttagc aaaagatcca aaaggaacaa
    40441 gtttttctga tgcgcatatt ttgtaatcaa ctaaggaggt gaagttaaat ggcagagagt
    40501 tgttattata gagtagctac agcaatacga atgattaatc cttctctttc ttccagaacg
    40561 ttttatgatt ggttgaatcg tattgaacaa gtaactgatt accgcttttt gcgcaaagaa
    40621 cgtgttttta caggaaaagt tattaatcaa gtattgctaa cgaaaaaaga tatagaacga
    40681 ttgactcgat tgtatcatta tcgagtagat ttagaagagg atttaacttt gtcaatttat
    40741 cgcgtttttt ctcctgaaaa atatagtgaa ataacaaaat tagatcattt aattttgtag
    40801 ttattatcta tcgggagggc tctagacgta gtctggtagc cgcactctgc ctggcggtgc
    40861 gcattaaggc tgcgaagcat gggcctataa tgcgcactcg ctagcagcgt gtgcagctca
    40921 caaagaggtc gtgcgcttgc gcacggcctc tttgtgtaaa gccccccgat atataatatg
    40981 gggggcagaa ataacataac acatagaaaa aagttttata aacccttttg tatcaagtga
    41041 ttatctaagg tcagcagttg tactactttt cccaaaaaac gagggggact agttgatata
    41101 tcggtgtttc taaagggtta aaaaaatcga ttttgttatt ttataaaagg agcgagaaaa
    41161 tgacaccgat tgagctaaaa agctacagaa agaagttaag tgcaattcat aatataagtg
    41221 gagataaaaa gccgctatca caagcaagat ttgcgcaatt gatgggctgc tcaaaagatt
    41281 acattagaaa gatggaacaa gggaaaagaa aaatcccaga ggattttgaa caacgaatca
    41341 aggaacgctt tcgattgtat atgaaaagcg gaatagatct tcgagcaatg attgattatt
    41401 tgcgtctttc cttttttggg acagactaca aaacagtgat tcaacgtgta ttacgaatgg
    41461 atctgacaga atttgaaaca cgtccaacgg gcttgtatct ttttgactgt ctttctgttc
    41521 gtgggaatat ctgggtgttc tggcattcaa aacgaactgt aacagaaaat gtacttgtgc
    41581 aattttcagg acaaggttgc cgggaatatg aggaagtcat gcgtgaacaa caaacagatt
    41641 ggcgaacatg gctatattcc atttggaaaa atcaaggaaa gttggaagct ggttatacac
    41701 gtgtgcaatg tagccgattc gatattgcgt tagatgaact ttggggattg gaaacggaag
    41761 aacatttcga tctctttgaa ttactagaaa aacaaaaagc aggtttatta gaaatggact
    41821 ttaagagatt ccagaatatt ggcggttgtt ttttagatga tggctatttt cgaagtgaag
    41881 gattatcttt gtattttgga tcgcgaaaga gtcctctctt ttttaatttt tatgagaaac
    41941 gttttgaaat tgcgaaccga gaaaaaattt cagaattaga agcattaacg aaatatggta
    42001 tttataatcg atatgagtta cggttagcca atgagaaagc aacacaagca gtagaagcat
    42061 ttattttagg tgattcaccg aaacgtttag gagagattgg cgttggttta attaatgcaa
    42121 aacttattgt ttgggataga ctggaaagtg atgcacggat tgttgataaa cgatgggcaa
    42181 aattatttcg ttcgatggaa gaattggatt ttacagtaaa gccagaaaaa ttctcattag
    42241 atcgttcaga acgctggttt ttgaaacaag tagctccgac attaaagttg ttacaagaag
    42301 cagatcgtat tttaggaaga gactttgtac aagaagggat tgataatgcc gaactaaatg
    42361 acgagcaaca aaactatcta gaatggatgg gaacgtcagg agggaaaatt gcatgaatgc
    42421 atatacgaca acagaatggc tgcgcctgtt ggatttaaag gctgcaatcg aagcattaaa
    42481 tgaaaagatg gttgatttgt cttattttcg ttttcgagtt ccttatattg aacaagcggt
    42541 aaaagctgga cgttatcagg aaaaagagaa ctggcaagaa attgcacgct tattagaagt
    42601 tcgtaaggga tacgaacaag aactcgaaga gttggaattt agccgaagga aaggaaggct
    42661 agaattcatt cgcttttatc gattttcatt gccaatacct gcgattttag cggttaaaaa
    42721 aggctgcgat aaaatgaaga tatatgaaaa ctgtgtggca gcacttagca gcgaaaaacc
    42781 tcttatggaa gaaatttcat tggtgactgt cacttgggaa atgagtcgcc agccaacaga
    42841 agaacagaca tatctttctt tagaagagat tgagatagaa ttagaggaaa ttggtcgcta
    42901 tgccacttgt tctacctatt gtggatcggt cattagtatt gctggtgtga ttgtctaaat
    42961 gaaaaaaatt atgatttatg ttggtgcgta ttggtcacgt gatccaacag tattagaaaa
    43021 tccagaggcg gtacactatt gcttgcgcca attattctat ctgtataaag aacgattaga
    43081 atcgttgatt cgtcagttac catatactga cagacgttta gatgaacttc ttctaaggta
    43141 tccagcaatg tataaacgac gaaaaaatag attgttacca gaagagtatc ccattgaaaa
    43201 acatgaactt gaagggcgat ttgtggctta tttctatgat gatgttcgta tgcgcttagt
    43261 agaacaacga atggaaatag aaaatgatcg ctactatttt atcaattatt gtaagaagcg
    43321 taagtatcaa gtaactgatg atttttatga ttgtatttta caagatgggg aagtgattct
    43381 atccaaaatt gctccaagtt ttcgtgaact tgtgccgatt gattttttga aaaaatgtca
    43441 tttgcggatt cttccgtaaa aaagagctat aaaaggaaag aaaagtagaa gccaaatcgg
    43501 tttctgcttt tttatttttt ttaggaagga gaagatggta tgtcactgga atttttagga
    43561 gagccaattg aaaaggaaga gttcgtttcg cattatatgg atttgtttat tcaggtgata
    43621 aaaaactttg tgcgattgat gaattttatc cacgcgaaca agactatcta gccgctgaac
    43681 gaataaaaat cagtttatta tgggaaaaat tcacagatga aaataaccga ccgccagatt
    43741 atcgctattt atcagattgt ttaacgaata atgtgctttc taaagaatac ctatttcaca
    43801 aacgtgagaa aaatcaaatg tcggttgaac atttttgtga ttgttatatt gcattattgc
    43861 gtagtcaaca gttattaaca gtagaacgat atggtgcaga gtttggttat gtcttgcaag
    43921 ctgagcgtca tagagctaca agatgctatg aaaaagaaga tcaagtgatt gatggctatg
    43981 aagaattatg tattcgaaac aatcgctatt tgcaacaacg tttatatgaa caattgatga
    44041 tggatttttc aaatctctat agagggtatg aatgttcgat gattcaagaa aggtagaaga
    44101 aaagggggaa tttttatgtg gcgattatta tttttggtgg tatgtggtat ggcattgttc
    44161 tatgtgtatc gtgtctatct ctatccacga atggaacgat ggacaagttg gtatagagat
    44221 cctttaaaac aacgaaaagg attgaagcgg tatcgttatg aattacttgt tgaattttcg
    44281 gatgggcgac aagcacagta ttattttttt gcaaactatc caaaatacaa caccagtgaa
    44341 ctattgattc aattattcaa aaataatcca tatgttgggt tagaagaaaa cggacgggag
    44401 ttcttttatc gttcagataa tattagtgcg gcgtctatga acaaaacacg aattgcataa
    44461 aaaattggag gaataatcat gaaaaaaaca acattagcag tcgtaacaac actaggaatt
    44521 tcattatttt cgtttcaatt agggagttca ttggtttatg cagatgaaac tgtagaaaca
    44581 cctaagtcag aaattgtaga aacattgcca ggggaaggat taccagatgt tgagacagag
    44641 ctaaatggag gaacaacgga aaaaccagca gaacctgaac aattgcagtc agagaaacct
    44701 gaaaaagagc cagtgacacc agaaccagag tttcctgaat caactttacc gactgattcg
    44761 atcgaagaag aaggtgtgca tagttcaaca acaacggatt caagtatagt cacaactccg
    44821 gaacaatcag gaacaaccag ttcttctgaa aagaatactg attcgtcaag tacggaacag
    44881 ccaacgatgt cgtctaccag tgaagaacaa aaagaagaac cgaaacaacc tgttgaaaaa
    44941 tcagaagcaa ttccaaaaga aaataaacct attgtttcag caaaagaagt cacagtatcg
    45001 gtaaccccaa gcggtgaaat tacgacgaat acttctcaag gaatgagtgt accgattgtc
    45061 actagtaatg tagaggaatt gacccatatt ccaacaccaa ccaccccatt aaaagtagca
    45121 ggtgatcaaa caattgttgg tgtaaaagat ggtattccat taatccaaga tagtgaaggg
    45181 aatttggtaa aggatttgtc gattcctgta aagaaacttc caagtggcaa tattgaaatc
    45241 aaaacagcag atggaaaaac aaaagtctta ccaaagactg gtgaagaaat acatgttgca
    45301 ctttctgttc taggaggagt gttgacaagt attgctggat ttgttggata taaaagaaag
    45361 agaaaaggaa tagaaaatga gggtaattga atggactatt tttcagaaaa acaagcttat
    45421 cgttgtcaat cgtacgtact agtaaaaaca tactagaagg agtagcttat gaaaaaacga
    45481 caactacggc ggaaagaaaa agtttataca gagaaagtac cgaagcaaaa gaaaatccat
    45541 tcaaaaaaag cagtgaaatg gatttggtgt ttggttttat ttctttgttt ttcagggggc
    45601 ttggcatttt ttcaatccat gaaaacgaag gttcaagtgt cgtttttgaa agatgaaatc
    45661 caacaattac aaaagaaaga agtacaaagt cggtcagaat taacgtacac acctgaaatt
    45721 gaatccttta tgaatcgttt tgttgctttg tatatgaata cattcgataa tcatgaacaa
    45781 gaacaacaac gtcaagaaac attaaaaaaa gagttttatg cacaaaactt tattcaagaa
    45841 ccaggaagtg tttcaggtgt tcgaaaatta actgctgctt cgtttattgg attaaaagaa
    45901 attgaaacca ttccaactgc tagttataaa gtaacttatg atattcgctc gatgaatcca
    45961 gagagtaaga aagaaacaag tgtgacaatc acacaagtac tcaatcttcc cttttctatt
    46021 tcaaacaagg gctgtcgtat tattgcttat ccttatttta cggcactacc aaagaataca
    46081 gaaaaacaac gaatgattac ttatgatgac acattatacg aaacggttga tgcgtcggaa
    46141 gcgaaagatg ttgaaggatt tgtggaagat ttcttagata agtatgcgaa aaacaatgca
    46201 aaagatatgt catacatgat gaaagaacct gaaggactca atcaaacagc taaagttgat
    46261 caagtagaga tgaaagtatt gcgttataaa gaaacgaaaa aaccttttat tgtaaaagca
    46321 acggtcgtct ttcgagatgg tgatacgtca cttttacaaa aagaacaaat gacgattgtc
    46381 attacgaaaa aaacaaatca gtattatgtt gaaaaattaa cacataattg gttggatagg
    46441 aaaggagaag aacggaaatg acactgaagg gaattgtaga ttttatatta acgcaaggtg
    46501 gctatttttt aataatggtg gttgtttatc tgggattgcg aaattttatg aaatcaaaag
    46561 taggaaatgc attaatatct atcgcagcag gtgccgcagt ttatttcttc ttaaatgatc
    46621 caacacgtgt atttgatgcg tttggtaaaa tcattgaaaa attatttatg gattaggagg
    46681 atagtgaagt ggattgggaa ccttatgatt attcgattgt ttatgcggaa ccttatcgcg
    46741 tacaacagat tacaagcaat tggcgtcttc catttccagt tgcattagtc aatgcagcaa
    46801 cttttttgtt ggtcgtaatc tttgaatatt tatttttacg ttcatttatt ggttgggtca
    46861 tttctttttt tccaccagca ggagttgttt tttatatagg actcccctat ttatgtgtgt
    46921 tagcactggc aaaactaaaa cctgatggaa aaaaagtgca tttttattta tgggattggt
    46981 tttgttatat gattcgtatg atgaatggtg attgtgtgta ttataactgg gacaaggtat
    47041 caaacaatgc attggaagaa ttactttttg tgaataagta tgtttattta gaggattaga
    47101 aaaagggaca atcaactggt tgttcctttt ttattgggag gtgaaattca ttggtgcttg
    47161 taaaagaacc agtaaaagca ttcaagaaaa attgtttgtt aaatggagag gattgtatct
    47221 gggcctacta tcatgttttt ccacgaacaa tttcaaaaaa cacaccaaaa aagacacagg
    47281 aaataaaaga aatgatggct cgtttactgt tgcttttagc agattatgaa gatgtggagt
    47341 tggtgatgta tccacgaaca atgaatttac ggacgaagtt tgacacatta ggaaagggat
    47401 tttctcccga tagtcaagca attggtgcgt attatgcaaa agaaacagtg acattgttgg
    47461 aacgagaagg tcaagtctat cgtccttcat ttattttagg aatcaaagtc aaagaaaaag
    47521 caacggtgga gagttttcat gatgtaataa atctaatctg gttagattta gcaggaagtt
    47581 tagcacgctt atttggatta gaagtagatg atttggaaac tttgttttct gaaagtgatc
    47641 cttatgtaaa agaagtgaca caattacttt atagcttttc tattcgtccc ttagtaaaaa
    47701 aagagctgtt gtatctcaat cgtctgcaat atattcgagg aatggctcac aaagtagaag
    47761 aacaaaataa ttgggaagta ctgaaagaca atcaaggaac gtatgtacgt ataccgaagg
    47821 aacataaagg ccttcttcaa ttagaagatg agttagggag tagttattta gcattgttgc
    47881 ctattgggga aacacctgaa aatatgagaa attgtgatct atttcaattt gctcaggatc
    47941 tttctttccc agcagaattg cgaatgaaaa ttcactatcc acgcttgaaa ggacagggag
    48001 gtattggttt aaaactgaat tggctatcgg aacggttcaa aacagagcat aaggaattgg
    48061 ttcaaaatga tgaagaacct agtgaacgct tattaactgt tcgaggatta gtaaaacatt
    48121 taaagcaacg agtagataat gggcaacctg tcgtcgattg gatcgcatgt tttattgtat
    48181 acggtcaaac ggttgaagaa gttcgtcaaa atagtgcaac ggttattcaa caacttcata
    48241 cgattatcca tatccaccgt gcaaaaaatg atcaacgaga attgttctat aaatgtttat
    48301 tagggcaacc attgggacgg cagttacatt ggaagcaccg aacaactgta gaagcagttg
    48361 gcgaatggtt gttttcagca gtatcagaat taggtatgga atccggctgg tatattggtc
    48421 ggcaagatac attaggggat ggtggttctc gaggagaaaa aacaccatta gaagaattaa
    48481 tttatgcgtc aaatcgtttt gtctttttga atcttctagc cattgcggaa ggaatcaaag
    48541 gtgcgttgtt tgatgcgcct catgtagcag taactggaaa aaccggtaaa gggaagacct
    48601 ttttagttgg cttgctcttt ttgtatagtt cgtttatgaa tgtacaaagt ttatttgtgg
    48661 atcccaaagg agaaaagcgc aaatggttta cgaaactcat tcaagacccg tattatcaga
    48721 aacattaccc tttgtttgta gaacatttgc aaaaaatgag ttatgtcacg ttagatgcta
    48781 caaatccccg taattatggt gtattagatc caattgtttg tttatcccga gtagatgcga
    48841 aacaagtagc ccaggatatg attgatgagt taagtccgtt aggtaccaat caccttttaa
    48901 aaggggcggt attaaaggga atccaagaag ttttagttca aaaagaagcc ggacaacaag
    48961 ttggcttgat gcatgtggtc gactatttag aacaaatgga acaaaaagag gttcgagaat
    49021 atggtgagtt tttacgcttg acggtagaag attcgatttt acgtttagga ttttcttatg
    49081 gagaaaaccc gggattagac tttaatgcca aaacaacgat tttagagatc caagatttga
    49141 aattaccaaa tgacaatgtc cgaccagaac tatatacaga tgcggatcga aagtctttgt
    49201 gtttgatgat tagtttaggt cgtttttgtg aaatgtttgg aaaacgagat agtagtaaga
    49261 aaacagcgat ttattttaca gaagcatggg tatttaataa ttcgaacgca ggacgtagta
    49321 ttattgcagc aatggcacgt gtggggcgtt cccagatgaa tcaattagtg ttagatacac
    49381 aatttattgg tgacttaggt tcagataaag aaaaagggaa ttttggtgtc gtctttgctt
    49441 ttgatgaaga cagtgagcga gaaaagattt tgaatcattt acgtttggaa gtaaatgaag
    49501 cgaacttagc tgaaatgagc cacatgatta aaggacagtg ttggatgtta gatccctatg
    49561 gacgtgtagg aaaactcaat gtacacagtt tgtttgaaga aatgaccgct gcattacaaa
    49621 caacggaaaa aacaagtcac tctcaagtag aagaacagga tacgttaatc atgcaataga
    49681 aatgaggtga aacaatggct tatataaagc ataaggaaaa gaaatggtgc gttttgtttt
    49741 ttttaacgat tggattcgga ctgctttttg ttggtggatt ggtgtatgcg gcagaaaagc
    49801 aggtggatca atcgatctat gagattggga aatttcatat tgataccttt tcttcttatt
    49861 tatctgatag tgaattttca ttaggcaaaa tgttatttgg ggcaaatggc acagattttc
    49921 taaaaatgat gacgaatatg ttctttagtt taagtaaatt gatttggcaa ggccttgatt
    49981 ttgtcattga gaagctctat gaaggagctg caatgaacga gttaattcac cagtttttca
    50041 ctttttcgga tactatttat tcaaaaatat acgatcctat tggtgcgggg attgtcgtct
    50101 tgtatgtgtt atatctattt actcttcgta tatttagagg gaaacattac gcaaaaatgg
    50161 tgtttattcg atttctcttc gttgtcattt tttcaatggt ttggtttggc tatggtagta
    50221 acgtacctag taaaggagaa gcttttacca aagggttaaa tgatttgtcc gtggaggtag
    50281 aaggctttat tttttctgct acaaatagtc tagatgaatt ggcgacaaaa gatgtcaatg
    50341 gtgttttagt gccaacgaca caaaaggaag ccattcaaaa agtgcgccaa ctttattata
    50401 aagcggctgt tgttgatccg tatatattgt taaattatgg gacaacaaat ttagaagaat
    50461 tgtatcaagc agagattgaa ccacaagaat ttctagccaa ggaagcgtca agtgaagaat
    50521 tatctgatat tgagacaaaa gtgaccactg ctgcaggaga agatagaggg aaaaagaagg
    50581 cttatcgtgt ctatattcaa ccaactaaag ctgtttataa agcagtcgtt ggttctatgg
    50641 tgccattgct taatctaagt attggattac cacttgtagt gattggcatg attcgtttta
    50701 tctttcaaat gttggtgttg tgtttattac ttgtgattcc tttttcatta ctcgcttctt
    50761 ttgttccagt atttgattat atgctcatga ataccttaaa gtcctttttg ggcttgatgt
    50821 ttcaaaaatc tttttacagt gtgattattt tggtggcatt tttgatctat aacatagtag
    50881 acgatttaat tccaatgaat actgtttttg gttttttagg aaatatgttt gtcaaagcac
    50941 tcctatcaat tttactcatt gtaaaacgga aagatttatt aaagaaacta gggttaggag
    51001 ctgcagatca aactataaac aacacgaaaa acatagcaag tcattctttg tctcaaatga
    51061 aacaactgcc tggtcaacta tcaactgctg gacaaaattt ggcaatcaaa ggtgcaggat
    51121 tagcagggaa tgtgtatcga gcaacgaatc cactcgcttc tgcagcgata catggatttc
    51181 aaaagtttca acaaaaacgt acaccacaag aatcggtaaa agggaggcaa gtggttaatc
    51241 cctttgaact aggtgggcgt tcaaatcaag ccttgcctta tagtggtcgt catgttcgtt
    51301 atcatggggt agagcctgaa atgtcttcta ccaaatcgaa aacagagtgg aattctggtc
    51361 tttcaaatgg tttgattcca aattcgtcca tgaattcagt aagaccaaaa caacaggtac
    51421 aatcattggc acaattgagt aagcaacaag ggaataatca aagaaaacca ttgttaaata
    51481 aaggtatcgc ttttgttggt caatcgattc cgttgccttc tggaaaagta cccgtgccac
    51541 aattaacagc tgctggttct ctttcacaac agattcgcca agtgaaaaat caggcgaaaa
    51601 atgggagtta tccagcaatg cagcagttat tgaaccaata ctctgttcct aattcgtcgg
    51661 tagccactct atcaggaaat cgttctgtgc aacaaccggg gcaacgttcg tctgaaagta
    51721 aaccgtcgat ttccgcacca aaatcgttac aacctgcatt ttcttcttta gataagccaa
    51781 cagagcaatc ggaaaaaata gaaaaggatc ctaaaacact gccgattttg gacgcaaaac
    51841 caggaactga ttttacttta ccaaaaggac gaacagtcca aaaaccatta tcgaagaaag
    51901 gatgaggaag aaatgaagaa agggtgtcta ggttgtcttc ttttctttat tcttcccttt
    51961 tttctattgc cattcttttt ttcaggagaa ctttctgagc atggaggaga agctacaggt
    52021 ctagctgtcg cttttaatgg ggaatatacc gaaggattac cgattttttc agagattaaa
    52081 ggacgtggtc caatcacaga tgaacatgcg caagcagcag ttggtgcagc tgtaaagtat
    52141 cagttattac cgagtgtgat tctttctcaa ttaggctatg aatcagctta tggacagtca
    52201 caatctggaa gaaacgatac gaactttttt gggattacct ggtttcctgg ttgtccctac
    52261 ccacaaggtt ctgcacgtgg aattggtggt tctgaaggtg gtttctatat gaagtttccg
    52321 aacttaaaag cttgttatga ctattatggc tttatggtgg cgagccaaag caactttaat
    52381 caatgtgtgg gcaataaaga tcctggaagt tgtttattga ttctaggtcg tggaggctat
    52441 gcggcagcag gtattaccga agggagtccg tattatcgag gagcaatgag tatcataacg
    52501 tcctataact taacggaata tgatactttt gcgatcaagc attggaatag cctaccaaca
    52561 aaaaaaactt cgcttattgc aggaaaaagt aaaggcaatg cgaaggtttt ggatcggatc
    52621 attggacgat ccgtaaataa tggggaatgt tatggtggta cggcatggta tgtatctcaa
    52681 ttaggtggcc cacagttaat gggttctgga cacatgtttg cgatgttaat aggggaagat
    52741 tataactgga caacctatgg ttggcaagta atcatgaacc caaaaccaag tgatttacaa
    52801 gcaggagatg tggtgaattg gcaagcaggt ggtgcattgt ctcctggaat ctatggtcac
    52861 accggaatta taacagatgt ttcatatggc ggacaacatt tctctagtgt ggaacagaat
    52921 gccgagcaag gacggattat ggctcggtat caacgcacgt acaatcaaac aaaaattcgt
    52981 agtctggttc gtaaggtgaa ataagaaagg cagtgactag aaacgtgaaa aaagggataa
    53041 ttttagtatt tagttggtta tgtttgatag gtcttactgg ttgtcattgg ttttcttctg
    53101 aaaaacaaaa aacacaattg agaacgacag aagaaacaat cgaagcagta aaagaaacaa
    53161 gtggaactct tcaaagtact gaaagtaaca gtcaaattgg ttcattagaa gacgagacag
    53221 cgcaacaaat gcaaatagat gaaatgacaa aaaatttctt aaaagatttt gctagaaaat
    53281 ggagtaattt cacagatgtt tataaacgca atcaagctgt gagggactat atgacagatc
    53341 gttgtattaa agacaatagt attgatgtag acccgcatgt acaaatgaaa acaactggga
    53401 aactttacca ggtgacacaa gatatagaga atccggatca atatttgtta tttggagaag
    53461 aaacaatgaa taaggttact caatttgtat tacttcaatt gaaagtaggt caagaagctc
    53521 aaaagattga tgcgatcaag atttattatg tacggagtgc gtattaaaat aaaaaatgaa
    53581 cactcatatt tattatacac cgaagaaata gaaaaaaaca gactgttttt ttaagaaaaa
    53641 ctatgtcatt ctttacttga aaaaagttgt tataggacag aaaggataaa acaaaaacgg
    53701 aaaaaatatg tcttatgaat aactataaga actattctat tttttaggag atggatacga
    53761 gatgaaaaaa ctaattggct atagtgtgat gtgtattgga tttgttggtg tgggattgtt
    53821 tgtatcgact gaatctgctt ctgcggctga atggtataat cctatctggt ataatccgaa
    53881 cattgaatgt caaaaaggct atccgtatgg accgacaaat ctgaaaaatg gaaaatgtag
    53941 agtaaattgg aatggcgttg tagatagtgt aattactggt tcaatggact ctgctctaca
    54001 tggctttacg ggtcactaga aggaggaaga aaatgtctga agagaaaaaa gaaatcgttg
    54061 aatcattagt agctttaaaa gattctcttt ctaagattga atatgttgat aggcaagaaa
    54121 ttcaaaaact aattgatgat actattattg aaatccaaga tgcgcgttgt gaaggtataa
    54181 aaatttctgt agctttatct aaagtaatag aaaaaatgaa ccgttcatta gcgtttaatg
    54241 gattgaagct agatagacaa actagtctga tatgggatca tttaaaagac ttgtatgaca
    54301 agtctaaaag aagtgaacgg actgctgtat caatcttaaa aggcctttgg ggaatgaatt
    54361 cataaacaag atatttaaaa agtaacttat tttttatgga ggagaaatta tgaaaagaat
    54421 aattaaaata tttggtgttt taggtgtttt cttattaggt ttaagtactt ttgttagtac
    54481 tggaacaagt gtgtatgcca cagaaaaagt gaatacagtt gatttagaaa taaagaataa
    54541 attagtagat gaattaactt tttattttga tacagttggt tttttagacg aacatggtgt
    54601 ataccatata aaggattttg gtgctattcg tgaacgaatc agtcaagata acgaacaagg
    54661 aagagcagca agagtctttt atgaacatat tattgttcct agaatggaga aagttgattg
    54721 gaaacgatat gctggatgtg tagccattaa ttctcttcct ggaggaggag tggcttgggc
    54781 tataggtacg cagggagatt ttattgaagc attaaaagct tttaattttg aaaaagctgc
    54841 ggatttatta ttaaaaattg cagaaaaaca attaggaaaa gctgcagcta aagagttagc
    54901 taaggtaaat attgtagcag gaattgcagc agctgctgct agctgtgcat tagagtaaaa
    54961 gtttatgaaa agtttagaac aagagcatat ggcttttaaa caagcaatgt ttaaagaaaa
    55021 tatctattta aaccataact atattcgtgt ttctaaggca tgttcaccag tattgaatat
    55081 gcttggaggt ggaaatggat tatatcattt actgtttgtc gatgtttgtt ggttaatttt
    55141 tttaccagat gaactagtta tagtcaatga aaaaataaca agtaaaaatg aagtgtttaa
    55201 ttattcttta acaagaatta attacaaaga gattaccaag ttttcactag aaaaagttcc
    55261 tttttgggga gaattttgtt taaaaattaa atgtaactgg aaaagaatgt atttctatat
    55321 tgatggtgat gatgctctta cattcggtaa aactactttt tctagtttta attttcagtt
    55381 tctattaaaa aataattttt atggtttatt gaaataaggt tgcgaaggaa gatgtagagt
    55441 tatttgcact gttgtgttgg tatatctctt aatgctgtgt ctttttgggg agattaatta
    55501 gtggagt
//
LOCUS       DASJSZ010000008        50965 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.008, whole genome shotgun sequence.
ACCESSION   DASJSZ010000008 DASJSZ010000000
VERSION     DASJSZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.008"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(56..1561)
                     /locus_tag="U4B03_000451"
     CDS             complement(56..1561)
                     /locus_tag="U4B03_000451"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEN2067328.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="U4B03_000452"
     CDS             complement(1653..2243)
                     /locus_tag="U4B03_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2067329.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="U4B03_000453"
     CDS             2422..4068
                     /locus_tag="U4B03_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2067330.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="U4B03_000454"
     CDS             4117..4836
                     /locus_tag="U4B03_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN2067331.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..6262
                     /locus_tag="U4B03_000455"
     CDS             5315..6262
                     /locus_tag="U4B03_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2067332.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6274..7194
                     /locus_tag="U4B03_000456"
     CDS             6274..7194
                     /locus_tag="U4B03_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2067333.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7235..8692
                     /locus_tag="U4B03_000457"
     CDS             7235..8692
                     /locus_tag="U4B03_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2067334.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9037..9651
                     /locus_tag="U4B03_000458"
     CDS             9037..9651
                     /locus_tag="U4B03_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2067335.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            9644..11368
                     /locus_tag="U4B03_000459"
     CDS             9644..11368
                     /locus_tag="U4B03_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2067336.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11365..12816
                     /locus_tag="U4B03_000460"
     CDS             11365..12816
                     /locus_tag="U4B03_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2067337.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(13289..15442)
                     /locus_tag="U4B03_000461"
     CDS             complement(13289..15442)
                     /locus_tag="U4B03_000461"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEN2067338.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(15444..16490)
                     /locus_tag="U4B03_000462"
     CDS             complement(15444..16490)
                     /locus_tag="U4B03_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2067339.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            16640..17929
                     /locus_tag="U4B03_000463"
     CDS             16640..17929
                     /locus_tag="U4B03_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEN2067340.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            17942..20644
                     /locus_tag="U4B03_000464"
     CDS             17942..20644
                     /locus_tag="U4B03_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN2067341.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            20629..21486
                     /locus_tag="U4B03_000465"
     CDS             20629..21486
                     /locus_tag="U4B03_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2067342.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(21557..22456)
                     /locus_tag="U4B03_000466"
     CDS             complement(21557..22456)
                     /locus_tag="U4B03_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2067343.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            22625..24796
                     /locus_tag="U4B03_000467"
     CDS             22625..24796
                     /locus_tag="U4B03_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN2067344.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            24864..25205
                     /locus_tag="U4B03_000468"
     CDS             24864..25205
                     /locus_tag="U4B03_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2067345.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(25481..27235)
                     /locus_tag="U4B03_000469"
     CDS             complement(25481..27235)
                     /locus_tag="U4B03_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2067346.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(27246..28082)
                     /locus_tag="U4B03_000470"
     CDS             complement(27246..28082)
                     /locus_tag="U4B03_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2067347.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(28084..28767)
                     /locus_tag="U4B03_000471"
     CDS             complement(28084..28767)
                     /locus_tag="U4B03_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEN2067348.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(28926..30320)
                     /gene="uxaC"
                     /locus_tag="U4B03_000472"
     CDS             complement(28926..30320)
                     /gene="uxaC"
                     /locus_tag="U4B03_000472"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN2067349.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(30359..31858)
                     /locus_tag="U4B03_000473"
     CDS             complement(30359..31858)
                     /locus_tag="U4B03_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEN2067350.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(31851..33272)
                     /locus_tag="U4B03_000474"
     CDS             complement(31851..33272)
                     /locus_tag="U4B03_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEN2067351.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(33332..34324)
                     /locus_tag="U4B03_000475"
     CDS             complement(33332..34324)
                     /locus_tag="U4B03_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2067352.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            34556..36754
                     /locus_tag="U4B03_000476"
     CDS             34556..36754
                     /locus_tag="U4B03_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2067353.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(37212..38084)
                     /locus_tag="U4B03_000477"
     CDS             complement(37212..38084)
                     /locus_tag="U4B03_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2067354.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            38384..39358
                     /locus_tag="U4B03_000478"
     CDS             38384..39358
                     /locus_tag="U4B03_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2067355.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            39380..40072
                     /locus_tag="U4B03_000479"
     CDS             39380..40072
                     /locus_tag="U4B03_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2067356.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            40038..41546
                     /locus_tag="U4B03_000480"
     CDS             40038..41546
                     /locus_tag="U4B03_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2067357.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            41620..42723
                     /locus_tag="U4B03_000481"
     CDS             41620..42723
                     /locus_tag="U4B03_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2067358.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            42726..44063
                     /locus_tag="U4B03_000482"
     CDS             42726..44063
                     /locus_tag="U4B03_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN2067359.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            44050..44928
                     /locus_tag="U4B03_000483"
     CDS             44050..44928
                     /locus_tag="U4B03_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEN2067360.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            44925..45848
                     /locus_tag="U4B03_000484"
     CDS             44925..45848
                     /locus_tag="U4B03_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEN2067361.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            45845..46255
                     /locus_tag="U4B03_000485"
     CDS             45845..46255
                     /locus_tag="U4B03_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN2067362.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            46259..47827
                     /locus_tag="U4B03_000486"
     CDS             46259..47827
                     /locus_tag="U4B03_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN2067363.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            48067..49485
                     /locus_tag="U4B03_000487"
     CDS             48067..49485
                     /locus_tag="U4B03_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2067364.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            49497..50807
                     /locus_tag="U4B03_000488"
     CDS             49497..50807
                     /locus_tag="U4B03_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN2067365.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT        16394 a         9009 c        10414 g        15148 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccctgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga
     5521 aaatttcaaa tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt
     5581 atataatgta gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat
     5641 gagcgagttg agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta
     5701 ttttctatct tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg
     5761 aatattcaat cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc
     5821 gaaatattgg ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag
     5881 cattatttac tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt
     5941 tgatggagct agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact
     6001 aatgacgatt ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt
     6061 ttataacatt cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta
     6121 tatctacaat ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta
     6181 ccaatcagtt gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga
     6241 tagcgattct gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc
     6301 caagtccgct ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt
     6361 gctatttcct gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc
     6421 atattgtcga ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg
     6481 tacttgttcg accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt
     6541 actgttttag gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca
     6601 cggtcaaact ttccattccg tcgttttttt accgtttttt gtttaattac gatgttgttc
     6661 tcaccaggga tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg
     6721 atttgggcat tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca
     6781 ttctttaaac gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc
     6841 gaactgcgaa tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc
     6901 agtttatttg ccgcattggg ttactggaat gactggttca atgctttact atacatccaa
     6961 gatgataagc tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat
     7021 ctgacacaaa attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga
     7081 gaatctgcca gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca
     7141 tttttccaga agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac
     7201 aaaaatataa atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt
     7261 tacaggtgtt tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa
     7321 agaccaggca gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc
     7381 agataatttc gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt
     7441 aaaagtcaat atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat
     7501 ttcttccggg gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa
     7561 aggagccttt gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga
     7621 tttagatgaa gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt
     7681 caatggaaat gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata
     7741 cgatctttca attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt
     7801 ccatgaaaaa gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg
     7861 gaactttgat tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga
     7921 ttcgaaaatc atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca
     7981 tagctggtac aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc
     8041 actagaagga aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga
     8101 tacaattttg acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact
     8161 aaaatctaca gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa
     8221 agaaaaatca gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct
     8281 tgtttggggg atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa
     8341 attgctagat ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa
     8401 gattctatat acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat
     8461 tgctgatgca gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac
     8521 agaattaagt aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac
     8581 tgttgatcca gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga
     8641 taaggtattg aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat
     8701 aaagacaaag gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt
     8761 tcaaaagcca gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc
     8821 acaaaatttt tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt
     8881 gttcaatcag ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt
     8941 tccgtttgtt tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa
     9001 tgagaaagta taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag
     9061 ttatttatcc ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt
     9121 gccggcggga tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat
     9181 ttgtacggat tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga
     9241 aattttttaa gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt
     9301 tacaatttat atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt
     9361 ttactttttg ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt
     9421 gaatatacga tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc
     9481 ttttcgtctt ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa
     9541 ttcaaaggat tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg
     9601 acaacacatt ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat
     9661 tctattttca aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat
     9721 cggtgtaacg atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa
     9781 acaggcagaa aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa
     9841 aacaaccttg agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa
     9901 tgtgacgacc tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca
     9961 aaacagtacc gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta
    10021 tgaagaacta agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc
    10081 tagagaaaac aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta
    10141 tatcaagttg cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca
    10201 aaagaaagaa ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata
    10261 catgatttct ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga
    10321 aacgtttgaa agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc
    10381 gattgacgat ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg
    10441 tatattagct gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc
    10501 gctgatcatt ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt
    10561 caaacgatat tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg
    10621 gaatttagag acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat
    10681 cggtatcaac gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga
    10741 aatcaaacag caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt
    10801 atataataca ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc
    10861 agatgttgtg tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat
    10921 cactttaaaa gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta
    10981 tcctaatcga gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc
    11041 taaatttgtg atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg
    11101 attcgataat gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat
    11161 caaagataat ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc
    11221 tcatcctaaa gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg
    11281 tgccagcttc ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac
    11341 cgtttctatt acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa
    11401 tacatgattt tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata
    11461 gtagcaacag cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat
    11521 ttagtagtaa cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct
    11581 caaaaagaac gtcataattt tgaatttatg attttatcag gataccaaga attcgattat
    11641 ttgaaaggag gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc
    11701 gaattggttg aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa
    11761 aatcaacaag aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat
    11821 gaaataagtg aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg
    11881 attcaactgg caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa
    11941 atcttttact atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca
    12001 gatgaagttg atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg
    12061 atcagtatcg gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg
    12121 aaagacaatc tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca
    12181 gtacaagcag aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa
    12241 aataaacaat tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta
    12301 gcagccatga tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa
    12361 cgggaattga ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag
    12421 gcaaaaacgg tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga
    12481 cagaaaaagt acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa
    12541 gaaccactta cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga
    12601 cagctgttca aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg
    12661 gaaaaagcga aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag
    12721 attggctaca acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc
    12781 cctaaagagt accgagaaaa cagtggagaa tcataaaaaa aaaagccaat gtaatgaaat
    12841 aacttataaa aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg
    12901 cagctttttc atgacctctc gataaacgga gttcaaaagc cagtttctcg gtattaagcc
    12961 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
    13021 aacagctttt tcacgacctc tccactaaat ggtgttcaat cagctgaccc tcggtattag
    13081 ctcaaaaaat ggcaaaatat gaggaactat tttgccattt gtctctctaa tactcaggtc
    13141 agaacgctga tttcacaacc tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat
    13201 aagaccaacc aaacgaaaaa tatgaggatc tatttttgct tggctgttct tattacttga
    13261 tgcaggacag ctttttcaca acctcttgtt aattatagtt tgataaggaa aaaggcaaat
    13321 cttttgcttg atatttctta gggtgaggga tgatccctaa ttggtaagag atcgtgccgc
    13381 ctttcagcaa atcttgatgg gtaaaatata atttcgtata ttctctgtcc atccgtttga
    13441 ttttatggat aaattgttgc tgcggccgat taggcgttgc ttcaatcgtc aattcctttc
    13501 catttgacaa aagcaaaaca gccttgtcaa ttaatggcat accgataaca tattcaccgg
    13561 ttccaggcgt gacaggataa aatcccagac tattgaagat ataccaagcg gccatacttc
    13621 cattgtcctc atctccaggg taacccattg gagaatcgtc gaaggtctga gtcatcaact
    13681 gtttaagcag tggctgtgcc atttcaggtt gtcctatata actgaacaag taaggatagt
    13741 gaaaacttgg ctggtttgaa atagctaatt gtccaaattc tatcgcggcc atctcactca
    13801 tttcgtggat ctcaaaacca tatcctcctg ggtcaaacgt tgggggctga ttgcacagct
    13861 caatgagttt ttctttatat ctctcactgc caccatattt tccgatcagt cctgaaaaat
    13921 cgtgataaac agcaaagctg ctttgccaag cacttccttc tgcataatct tgtccccaac
    13981 gattagctga aaatggttct ctgaacttgc catctttatc ttttgaacgc ataaatcctg
    14041 actcatagtc aaagatattt tgatagttca atgattgctg ctgatatttc ttcgtcattt
    14101 cctcttcttc tagtaccttg gcaacttggc taatacaaaa atcgctataa gcatagtcta
    14161 atgtgtgatt gactgattcg tggtagtgat taggaacata gccatatgtc aagtaatctt
    14221 ctgttccttg gcgtccataa tttggatgtt tgctttgaac cgtagcacct ttttccattg
    14281 ccgttagaaa ttcaggcatc aagtctcgtc ggatatcttt tacagcagca tctgcaatga
    14341 ccgcatcgat caatgtccca ggcataagtc cacgttcatc aggagacagc cattttggca
    14401 aatagccact ttctctatag ctgttcaaaa aaccttccag catttcttcg tatttttcta
    14461 cagcaatcaa agaataaaga ggataaaccg ttttatacgt atcccaaaac ccattatttg
    14521 tatagagaac accaggtttt atagatcgac tagtcgtatc atagtggatt ttttgcccac
    14581 taggatcaat ttcataaaat gtttgtggaa ataaaaacac ccgatataaa ttatgataaa
    14641 acgtccgcaa ttgttgttga tcatggtgtg tgagctgtat acgatcaaaa taggactgcc
    14701 attgttcttg actgtgagca aggtaagtct ctttgctttg gttttgttct agttccaggt
    14761 tataagctgc ttgttccgag ctgataaaag atgtcccaaa tcggatagtt tgtttttttg
    14821 tctgttcaaa atacaacggg atcgagtcgc tatttttgtt ggaaacagct aactcatcaa
    14881 ttgttaaagg atattcaaat ctcaatataa aataaaaaga aaaatcagga tcttctgaac
    14941 cagcaaaatg tgtgattttt ccttccactg tgtaaggatc tataacaaag agctgatggc
    15001 gtcccggaaa ggataaaagt aaaccgctgt catttttgct gtaatcaata gataagattc
    15061 cgccatacat gctaggtatc aatgtagaac agatgccata acgtaattga cagatcgaca
    15121 gatgagtcgg gcaaaatata ctttcttctg ggcggtaaga actttgagca tggaaaaggg
    15181 tgttctcctg tagttttcca ttgatcggcg taaacagcat atgactgaaa tcacccatcc
    15241 aaggacttgg ttgatgggta agacggtagc cttgaaaaac gcggtcatca ggatgaaacc
    15301 accagctgcc gttttgatcc gtagtttgcg gtgcaaaata attcatccca aaaggaacgc
    15361 cagtataagg ctggcagttc ccattagaga aatttggctg attcgctgtt gcatgtctag
    15421 tatctatttg accgatattc attttatttc tcctttgttg attgtctttt gatgaatgaa
    15481 actggaaatt tactatcgga tggtcgatct tttgtctcta accaagtgac caaagcatct
    15541 cccgcaagct tacctaactg ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca
    15601 agagctgctg cttggatatc atcaaatccg ataattgaaa aatcttcagg gatatgataa
    15661 ccatgctgtc tcaagagatt catggtctgg atagcgacaa catcgttttc acaaacaaaa
    15721 gcagttactt catacttagt gatataagaa agcaattcat ctaatgtcgc gcttttgtca
    15781 tccagtgaag tgtggaaagt caaaccagct ttgtccaatg cttcaaggta accaaggtaa
    15841 cgctgtcgaa cggattgtgg atgagtctgc gtaccaaaca aataagcgat ttttgaatgt
    15901 ccctgttcga tcaaataaga ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg
    15961 gtaggaaaag gtaactcata gattttttta tctaatatga cgacagggaa ttctttcaat
    16021 gaaagctcaa aaagtaagtc aagatgttgt tctgtatcaa gagcataata aatcaatccg
    16081 tcaaattcat tgattagctc ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc
    16141 atgacatcaa tctgtttatc ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca
    16201 gacaaatcag cgacatatgg aagaagaaac agaatccgtc gtgtttttgt aggagaagca
    16261 agaatctgtt ccggagaagc ggatttaacg aaactgcctt tcccgcgaac gcgataaata
    16321 agtcctaatt gctctaattc agtcaatgca cgtttggaag tgattctgct tactttatag
    16381 gtacgtgaaa gttctttttc agttggtagt tggctgtcta caggtaattc accagttaaa
    16441 atagccgttt gcaaatcatt aaatattgtc tggtacaatg gttgagtcat ataaacacct
    16501 cttaatgata tatcatattc aaataatacc ataatggatg aaaaagtaaa taaaaagata
    16561 ttttttgaaa gcgtagacat aattggctgt atgaagtata atctttaaag atatatcaaa
    16621 tagaaagaag gttttattag tggtttataa agatatacca aaatcggtgc agaaatttat
    16681 ggaagatatc acacaaaaat gtggggatat ccatgcggac tgggcaaaga attttacagc
    16741 agcatttgcc aatactctgt tgacaacagt caaaaagcaa aatgacggaa ccaccttttt
    16801 attgacggga gacattccag cgatgtggtt aagagactct actgcacaag ttcgtcctta
    16861 tttagtgatt gcaaaagaag atgaggaatt agctgacatg atcagcgggt tagtcaaaaa
    16921 gcaatttttt tatatcaata tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg
    16981 tgctggccac caaacagatc atacaaaaat gaacgattgg atctgggaaa gaaaatatga
    17041 aattgattct ttatgctacc caatacagtt ggcctatctt ctttacaaaa atacaggaaa
    17101 aactgatcaa ttcaatcaat cttttattga aggtatcaaa aaaatcttgg acgtgttcaa
    17161 aacagagcaa gatcatgaac actctcctta tacatttgta cgagacacga caagaaaaga
    17221 agatacgctg atcaatgacg ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc
    17281 tggtttccgt cctagcgatg atgcttgccg ttatggatat ctggtaccat ccaatatgtt
    17341 tgcggtggtc gtgttaggtt atgtggaaga aatttttcaa acgatactcg atgataaaga
    17401 aattgtacag caaacacaga aattgaagaa agagatccaa gaggggattc agaatcacgg
    17461 acgaacggtt aatcaaaaag ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa
    17521 cgccacagtc atggatgaca gtaatgtgcc caatctgatc gccgcacctt acttgggata
    17581 ttgttctgaa caagaggaag attatctgca aacaagaaga acattgttga gtaaagaaaa
    17641 tccatatttc tatgaaggaa agtacgcaaa aggaattgga tcctcccata cgccagaaaa
    17701 atacgtttgg ccgattgcat tagctatgga aggaatgaca acgtcagaca aatctgaaaa
    17761 agagcggata ttgaatttac ttgtagcaac tgacgcagga acacacctta tgcacgaagg
    17821 attcgatgtg aacaatcctg aaaattatac gagagaatgg ttttcttggg caaatatgat
    17881 gttttgcgaa ttagtcatgg attactttga tattagagta gaaaagtagg aggtatcgta
    17941 aatgaaaaag aaagtatata tcatttctca tagccactgg gatcgtgaat ggtacatggc
    18001 ttacgaacag catcacatgc gactgattga attgatagac gatctactag agctatttga
    18061 agttgatccg tccttcaaca gttttcattt ggatgggcaa acgattattt tagatgacta
    18121 tcttcaagta aggccagaaa aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt
    18181 gagaattgga ccattttata tcttgcaaga tgattttctt atcagctcgg aatcgaacgt
    18241 gagaaatatg ctgattggca tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg
    18301 ctattttccc gatacatttg gaaatatggg acagacgcct caattaatga aacaagctgg
    18361 tatttcagca gcagcgtttg gccgtggtgt aaaaccaatt ggctttgata atcaagtttt
    18421 ggaagcagaa aactattctt cccaatactc agagatgtgg tggaaaggcc ctgatcaaac
    18481 agcaatcttt ggtctgctat ttgccaattg gtatagcaac gggaatgaga ttccagtaga
    18541 aaaagaagca gctcttgcct tttggaagca aaaattagcg gatgcagaac aatatgcctc
    18601 tacgaaccac ttattgatga tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc
    18661 aaaagcaatc catctcgcaa atgaactgtt tccagattac gagtttattc attctaattt
    18721 tactgactat ctggaagcag ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg
    18781 agaattgaca agtcaagaaa cggatggctg gtatacttta gccaatacag catctgctcg
    18841 ggtttactta aaacaatgga ataccaaggt ccagcgacag ctagaaaata ttacagaacc
    18901 acttgcgacg atggcttatg aagttagtgg aaactatccc catgatcaat tagattatgc
    18961 ttggaagaca ttaatgcaaa atcatcctca cgacagtatt tgcggttgtt cagtcgattc
    19021 tgtccatcgg gaaatgatac ctcgttttga aaaagcagat gaagttggaa agtatcttgc
    19081 acaagattcc ttagaacagc tgaccgcagc aatcgatacg accggattcc caaaagacag
    19141 ctttccattt gtcattgtaa atactgcagg aatggataaa actggtgaag cagaaataac
    19201 catcgaatta gaaagaaaaa gattcgctga aggaatacct gaacaactat atcaagagtt
    19261 agaaaatttg cctaaacgta agtatcatgt agaaacaaaa agtggggcca ctataccagc
    19321 tattttatct gaagaaactg tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt
    19381 gccatatatg gcaagaatga tcaaagtgac tttaccatta gaaaacatgc cagcattttc
    19441 ttgggaaaca ttcgcgcttg tagaagggga agcgaaagca gaagaaaagg aaacaatgat
    19501 tcatcagagt ggacgaatca tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg
    19561 taccattaca atggaagatc gcaaaaacaa acgaaagttg aataatctgc atatttttga
    19621 agatatcggc gatataggaa atgagtatat ctttaaacaa cctttctgtg atcagccaat
    19681 cctatccagt aacaaagaaa actctgaagt aaaagtatta gtagatacac cggaaatagc
    19741 aaaaataagt attctacagg aaatggaaat ccctgtttct gcagacgagc gtttagaaaa
    19801 agaacagcag atggttgtgg aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg
    19861 aatccttcaa ataaaaacaa tcatgacaat cagaaaagat agtaaaaaaa tcgactttga
    19921 aacgactctc gacaatcaga tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt
    19981 acatgtggaa catcacgaag cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt
    20041 gtctaaaagt tgggaaaacc ccacgaatcc gcaacatcag caagcatttg tcaatattca
    20101 tgatgaagag tacggtgtaa cagtcggaaa ctttggtttg aatgaatatg aagtaacaga
    20161 agacggacaa atcgcagtga cgctacttcg cagtgtggga gaactaggcg attggggata
    20221 ttttccaaca ccagaagcac agtgtctagg tgagcatcgg ttcaattaca gcattgaact
    20281 acatggacca gaagaaaaat tctctactta tttgcatgca tacgcagcac agattccttt
    20341 ttccactcaa caaatcaaac atcatgaagg aacattgatt tcaaaacaac aatatttaac
    20401 gataaaaagt gaaacgtttg caattacagc tttgaaacgc agcaagttta gtgataaagt
    20461 agtagtaaga ggtttcaata tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa
    20521 tggaaaaact gtcatattga atctgcttga agaaccaacg aagcaagcgg ttgtcccaat
    20581 aatccaacct tatgagattc ggacaatcgg attcgaggag gaaaacgaat gtatggcgga
    20641 atagaagcag gcggaacaaa atttgtgtgt gcggtttcag atggtggaga aattttagaa
    20701 aaattaagta tccctaccac aacaccagaa gaaacattgg atcaggtatt tgaatttttt
    20761 gatcgttatg aattgaaggg aatcgggatt ggctcattcg gcccgattgg tatcgacaaa
    20821 acaaaagata attacggata cgttttggca actcccaaaa aagggtgggc acaattcgat
    20881 tttcttggca caatcaagga acgctacaat attcctgctg cttggacaac ggatgtcaat
    20941 gctgctgcat atggggaatt gaagcaagga gcagcacaag gaaaaaatag ttgtatttac
    21001 cttaccgtgg gaacaggaat cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg
    21061 atcgcacatc ctgaaatggg acatatttgg gtgaaacgcc atccagatga ccattatgaa
    21121 ggtacttgtc cttatcataa agattgttta gaaggattag cagcaggacc gtctattgag
    21181 gcacgcacag ggatcaaagg gcaagatctg ccgcaagatc atcctgtttg ggctattcag
    21241 gcttattata ttgctcaagc actgatcaac tatacgttga cgttagcacc tgaaaagatt
    21301 attttaggcg gtggggtaat gaatcaggat catctccttc aaaaaatccg tcagcaattt
    21361 gttgaattga tgggtggtta tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg
    21421 ggactgccga atgaaagtgg gattatcggt agtttgctgt tagcagaaca cgaacatcaa
    21481 aagtaagcag acaaattaca caaaaagaac agagtcttat cattatctgc tgataagact
    21541 ctgttctttt ttatttctat tcccataatc caggtaaagc tttaccgact attttttctt
    21601 gagtaaatgg atggatcatg tgcagttcta atgcatggag catcagtcgt tcagctgttc
    21661 tattttggta gagagggtct ccaacgatcg gatgtccctt atgagctaaa tgaactctga
    21721 tctggtgtgt acgtccggtt tccaaaacac aatagacttg tgtttgttca gaaagtactt
    21781 tgttgacagt gacatgtgtc aaggcgctct gaccattttt atcatctacg accctttttc
    21841 tccgatcatg gcgatctcgt ccaattttat tggtgatggt catatctttg gcaatttctc
    21901 cccatacgat ggcttgatat ctccgataga tcattttttg ttccaacatt cttccaagta
    21961 tcggcaagac aaatggattt tttgcaaata aaacggcacc gcttgtttct ttgtccaagc
    22021 ggtggacaac gtaaggtcgt tgctgtttag tgtcgagata agcagctaaa tgattgagta
    22081 aggtactgtt ttctgtcggt tcatttggat gagtcttcat tcctgcaggc ttatccacta
    22141 taatcaaatg ctcatcttca tataaaacac ggatgtgctt cgcatctcct aattgaactt
    22201 cttgataatt ataatctgtt tcttcgaata acaatgtgat ctggtcattg ccctttactt
    22261 catgatggaa caaagcaggt tgttgattga tccaaacgtt tttacgtata cgcaaaaagt
    22321 gtcgaacctt tctaggaact aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag
    22381 gatggttcat aggtaaagtg atcgtgattt tcacaagtgt actcctttca gtgcaatcgc
    22441 agactaattg taccattttt aaaacatatt taagaaaaaa cttacatttt tctgttcatt
    22501 cttatgttac tctacttgca ctaacgtaaa aaaacatatt gaactacaac ttaagcagga
    22561 agaaaacact ctttcactat ggtaaaataa gacgattgaa tcaaatagac aggagaagaa
    22621 gcaaatggat tttcaaagca ttttagaaaa gataaaagct gctttgatca gtttttggcg
    22681 ttggatcaga ccatatctcg gacaattcca tcgatggcga aagcgcatct ggaaaaaata
    22741 tcatgtgaac aaattgattt tgctgattgg tttgatctgt gtcttagtga caagtatcta
    22801 cctctttatt ttagcaaaac aagcaaatgt cgaaacgtta aaatcaggat taagccaatc
    22861 gacagtcatt tatgataaga aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac
    22921 atatgtagaa ttaaacgcga tttctcccta tatccaagat gcagtaatct cgacagaaga
    22981 taggaatttt tacgagcatc acgggtttga tatcaaaggg attgcccgtg cagcagtccg
    23041 aatggtcttg tctggcggaa catcaggggg cggaggaagt acgatcactc agcagcttgc
    23101 caaaaatgcg tatctgacat tagaccaaac gtttgatcga aaagcgaagg aattattttt
    23161 ggcaatcgag atcgaaaaga agtacagcaa agaagaaatc ttgaccatgt atcttaacaa
    23221 tgcctatttt ggcaacgggg tatggggtgt ccaagacgct tccagaaagt attttggagt
    23281 agatgcaacg aatgttacgc tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc
    23341 tggtatttat aatccgatcg atcacccaaa aaatgcgaat gatcggaaaa acacagtact
    23401 aagtgtaatg gaagaaaacg gaaaaatcac aaaagaacaa gaacaagcag aaagccagac
    23461 agatatcagc gcgtatctta atgacacata cgataattca gattctggtt atcgttatcc
    23521 ttattatttt gatgcagtga tcgatgaagc agttaacgag tatggtcttg atgaagaaga
    23581 tgtcatgaat aaaggctata aaatctatac agctttagat caggattatc aaaagcaatt
    23641 agaagctact tatcaaaaca gtgcattatt cccagcaaat gcttcagatg gtgccatggt
    23701 ccaatctgct tcagtagctt tagatccgaa tacaggtggt gtacaagcac tagtaggacg
    23761 aagaggagat catgtcttta gaggattcag ctttgctaca cagatgcgtc gttcaccagg
    23821 atcaacgatc aaaccattaa gcgtatatac accagcactg gaagctggat acaagccttc
    23881 cagtatcttg aaagacgaac cgcaaagtta ttacgatgcg cataattttg acgggacata
    23941 ccaaggagaa gtaccgatgt atcaagctgt tgcacaaagt ttgaatttgc ctactgtatg
    24001 gcttttgcat gagatcggtt tacaaaaagg atatgataaa gcagaagaat tcggcctgcc
    24061 tttagcaaaa tctgataagt attatggctt agcccttggt ggactggaaa aaggagtatc
    24121 tccattgatc atggcgggtg cttacagtgc atttgccaat gatggtcaga tgtatacgcc
    24181 gcatcttatc acgaaaatcg ttgattcgac tggagcggtg atcgtcgata acacaaacaa
    24241 aaaaccaaaa caggtcatat caaaggaaac agccgatgaa atgaccagca tgcttcttgg
    24301 tacattttca aatggaacag cggctgctgc gaatcctgca ggttatacga tagcagggaa
    24361 aacaggaacc acagaaacta atttcgatgc cacaaaagca aatgaccaat ggatgatcgg
    24421 ttacacgcca gacgtagtga tttcgacatg gatgggttac gaagaatcca gcaagctgca
    24481 ctatttggaa gggacaagtg gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg
    24541 catccttccg tatacttcaa aaacaggatt tagtgtagca gatgcctatg ccacaggagg
    24601 acaagtggta gctgcagatc aagttggaaa cacaggaaat tctaatggag agtctggaaa
    24661 ttggcaagat aacttgaatc aatatggtga acaagcaaaa gaagggctga aaaacttcgg
    24721 cgatatggtg aaagaaggcg ttcagggaat tggtgaagcg gctaaagacc tctggcggaa
    24781 atatcaagga gagtagcccc tttgaaaacg aataacccca tgttacaata gagagtaact
    24841 actaaaagaa aagagggcat aacatgtcaa acatctacga tagcgcgaat caaatcgaac
    24901 gtgagatccg tgaattacca gaattcaaag cgttgaaaga agcgtacgag aaagtacaag
    24961 caaacgaaga agcacacaaa ctgttcaaag acttccaagc gatgcaaatc gaattacaag
    25021 aaaaacaaat gagtggacaa gagttcagcg acgaagatgc aatgaaagca caagaaatgg
    25081 ctgtgaaaat ccaatcagaa gaagtaatca atgacttgat gcaaaaagag caaggcttca
    25141 gtacaatcat caatgacttg aaccgtatca tcatgacacc agttcgcgac ctttacagcg
    25201 aataaaacta ttttatgaaa caacagaggg tgtgaaagag acgttcggcc ttgagcgatg
    25261 acaaaattgc ttcttattgc cgataggctg tctcttgaac accatttacc aagaggtcgt
    25321 gagcctaccg tttaactctg agttacaagg aacaatttca aaaaacagct tctcatgttt
    25381 gatgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta ttcagaaata
    25441 ggagctacga caaaagtctt gtcatagctc ctattttttg tcattcattt ttcaagcggt
    25501 agaaatagcc tttttgagct aacagttttt cgtaagtacc tatttcaacg atttttcctt
    25561 catcgatcac aacgatttta tctgcctctt tgatcgttga aagccgatga gccacaataa
    25621 aagtagtggg tgtttctgtc aggtaattca gggcttgctg gatttttttc tctgactggt
    25681 tatctaaagc tgaggttgct tcatctagca aaatgatttt tggctgacgt atcaacgctc
    25741 gtgcaatgga gatacgttgc ttttggccac cagacagctt gttgccatgt tcgcctacta
    25801 aggtatccag accatcagga agctggtcaa tcagtgaaga aagttgcgca gcttcgatga
    25861 cttcatctaa acgttctttt gatacgtttt tcaagccata agtgatgttc tctttgatcg
    25921 aagcggagaa caacaaagtg gtttgaggaa caacggctaa ataatttcgc acacttcgca
    25981 tatccagttc atccaaaggc tgatcatcta gaaaaatttt tcctgaagtc ggctggataa
    26041 atccaatcaa taagttaagg agtgtagatt ttccagagcc agatggccca acaaaagcaa
    26101 ttgtttctcc agctttcacc tctagagaaa aatgatcgat aatcggtttg agtgagtccg
    26161 gataaagaaa agaaacatcc tcaaaacgaa aatttccatc caaatgttgg atttttttct
    26221 taccgtgata agtttctaca tcgccagcaa tcaacagttc gccaatagaa gtgatagatt
    26281 ccatgccttt tgaaatcgta ggaatcagcg tgatcattcc agaaacggaa ttgacgatcg
    26341 tcgtgaaata cgtctgatac atcacgacat cgccgggttg gatgcgccct tgtaaagcta
    26401 atatcccagt gaaaatcaaa caaatcagct gaaatatctg aaaactagcc cagctgactg
    26461 atccgaaaac agattgcagg atatctaagt ggaatccttt ttcatagatt tgagtaaaat
    26521 gcttatttaa gcgagttgtt tcgtgttttt ctaacccatg agctcgagca atcggtgcta
    26581 attcgatcac ttcgatcact tttgcagaag tctcttccat ttcttgcctg aagtctgagt
    26641 tggactgtct gatcctttta cggaaaaaga gagtaacgaa tgcggcgaga ggaacagtca
    26701 ataagaaaaa catgaaaaca acaggacttt tataaagcgt gatacctaaa gctacgaata
    26761 aattgacagc gatatttaat ccgctgacaa atacttgaga ggacaacgtt tggatagctt
    26821 ctacatcacg gatgatcttt gactggattc gtccagattg caattctttt tgataaggaa
    26881 tagatagttc ttggattttc ttgataaggg tcgctcttag tccagcttct acttgacgga
    26941 tcgaagtgct atggtatttt acatgaaaat aattcgtcaa tatattttga ccaatcagac
    27001 aagccatcaa aaaagcgttg aaataaatgg cctgcaactg agaagaatct ttcgatgaag
    27061 ctgcattgat tacattggca atagcgatcg gcattaacca agatggaaga tgtttgataa
    27121 taaaaaagag tcctgacatg gcaaaacgcc aataatttcc ctgataaaga tgcagcaatg
    27181 tctgaaaagc atttgcatga tatttttgat aacttttctg tattcgttcc tccataatct
    27241 tctccttaac aatcttggta aataaactgc aagtgttcta ttgcttcagg aacataaatc
    27301 tcaggagtag cttcaagagt ggcgtacatt agaggacgct catatttgat ataatgcagt
    27361 accgggcgga aatctaaaca gcccttgcca acaggtacca tgcggatctt tccattttcc
    27421 atgatatagt ctttgagatg aagcgctgta atataaccat gtaaattgtc caaggcacct
    27481 cggatcactt tttcctgatc agcatgattt tctttatgca tcaaattcgc acaatctaaa
    27541 atgattttta aatttggtga agctacttca tcgattaaac gtttagctaa ctgatagtta
    27601 tagattggat gattgatccc tgcttcaaca gcaacagtta cgccaaaatt ttctgcatga
    27661 gcgactaatt caatgataga atttctggcg atctgataag cttcttcagt aaaattttct
    27721 tttgtgtaac cgacagagac actaccggtt tccgtagcaa tcatcttggc ttgataggca
    27781 gagcacagag caatttgctg tttgaaagtt gccaatgctt gtgcacgaac ttcttgattt
    27841 ttgctggata ggttgatata gcatcctaaa atggaaagtt cgatattgtt tttttggaag
    27901 tattcaccaa aataggcagc actcccaagt gtcatgtcag ttaatttaag ttgttcagga
    27961 aatgcttttt gtggtgcaaa ttggatatgg gacaagtgaa gctgtgatac tttttgaacg
    28021 atttcttcac gatttcgaac atctaaatcg tgggcacgga tgcctaaatt taatggaaac
    28081 atctcaaact cctttttgta aataattagc aagaggcaga cgggattctt ttatcaattc
    28141 aacaattatt cgggcaactt tatcagcgcc ttctggacta aagtgggtat tgtcttttag
    28201 tccttctggg taatttttac aagtatcagg tataagatgc aggtagaatt tttttgtctc
    28261 ttcttttgag aaggtctgga acaattcttg tgatcttgag aacacatcta aaagaggaat
    28321 atggtgtttc tcagcaaatg cacgcatcgc ttttggatag tctccaagta tatctggatt
    28381 caatgttccg ttttctaagt aatctcgtcg cgtcacagat gtcagtaaga ccatttggac
    28441 gttcgctttt tttgcgcatt caaaaaatat ctgcagattt tttaaatagc taccgaaggg
    28501 aagcgtacct ctttcttctt ggattttctg gtcgttatgt ccaaattgta caaataagaa
    28561 atcattcggc tgaaactgtg aagctagacg ggctaacctt ccttcctgga taaatgattt
    28621 ggtgctgcgt ccattctttg cttggttatt gatctggatc tcgggtgaaa aataagcaga
    28681 gattttttct ccccaacctg tctcgggacg cttgttttca tctttttctg ccgcagtaga
    28741 atcaccggca atatggatag taggcatgtt atctcttctt tcataaaggt ttataggcaa
    28801 tgaagcagaa cagcaagaga agaagacgga gcaaatttgg tccgtcttct tctctaaaac
    28861 gtggataaac ggattccgtt atttcactat cctgtttttg attcgttact gttactatta
    28921 atcaattagg caaaaaagcc aaagtaattt ttagcatttg tataactaat cgcttgaact
    28981 agacttccta aaagttgttc atcttcaggt gcttctcctc gcatgaccat ttcgcctaaa
    29041 aattcacaaa gaacacgacg gaaatattca tgtctagtgt aggacaggaa acttcttgaa
    29101 tctgtcagca tgccgacaaa attccccaaa gcactaccat cagcaaattg tgtcaattga
    29161 tcgcgcatac cagctcgagt atcgttgtac caccatccag aacctaactg cagttttcca
    29221 ttctgttctt tttggaaaca gcccatcaaa gctaaaatcg caggatagtc attcggattc
    29281 aatgaataaa ggatcgtctt tggcaactga tccgtttctt ctgcacggtt caataatttt
    29341 tgcagaggaa tagtcaaaga aagatcattc attccatcat agccggtatc gggccctaat
    29401 ttttccaaca tttgtgtatt acagttacga taagcatgga tgtgcagctg catcgtccaa
    29461 ttatgagaat gatagaagtg ggtcaaatgt atcagtgttt ctgtacgata agcatcgatt
    29521 tcatctgaag ataatagttt gttagctcgt gctttttgaa aaatctcttc taatactgtt
    29581 tcatccgctt ctttatagcg taagatgtct aaagcgtggt cggaaacacg acatccatgt
    29641 tgatggaagt attctatccg ctgccctaag acttctatca gttcttgata ggtggtgatc
    29701 gtttggtcag ctgcttcttc cagtagtgtg acccatgcat taaatccttc tttttcaata
    29761 ttgagtgctt tatctggacg gaatgaaggt agaaccttca aatctggttc ttcatgagca
    29821 agcaactgat gatagtgcaa gtcgtctgcg ggatcatctg ttgtacaaat cacttctaca
    29881 ttcgattttt ttgcgatttc ccgacggcga aaatcaggag ctgccaaaat ttcatttgct
    29941 tgtgtccaaa ttttctctgc attttcttca tttatcaaca agtcaatacc gaagaaacgc
    30001 ttcaattcaa gatgtgacca actgtaaagc ggatttccga taatttttgg tacggtttga
    30061 caccaagcta aaaatttttc aaaatcagaa gcatttcctg tgattttttc ttcaggcacg
    30121 ccgcaagctc gcatcaagcg ccatttgtaa tgatcgcctc ctaaccaagc ttctgtcaga
    30181 ttgctgaaat tgttgttttc gtaaatttct tttggatcta agtgacagtg ataatcaata
    30241 atgggcatgt tttttgcgta ggaatggtaa agctttttcg cccaatcgtc ttttaataaa
    30301 aaatcatcag ataaaaacat aaatcttcct tctttctttt tatactgttt tatgaaaatc
    30361 aaagtgttac tccgttttta aaaatggcaa tttctcgaac gtcattttgt tcatttttcg
    30421 tttcttctcc actagctaca gcgatgattt tttgaacaaa cgattctaag attacttcca
    30481 ttggttgttc taataagacg cctgcattaa aatccatcca atgacctttg cgttcaaaaa
    30541 gagttgtatt cgtagatacc ttgaccgttg ggacatatga gccaaacgga gtccctcttc
    30601 ctgtggtaaa caagaccaac tggcaatcgg atgaagcaag agcagaggca gctacaagat
    30661 cattcccggg agcttgcaat aaactaagcc ctggttttct gattttttcg ccatattgta
    30721 gcacatcaac gacaggagag cttcctgatt tttgcgtgca gcctaaagac ttatcttcta
    30781 atgtagtgat cccacctgct ttattaccag gagatgggtt ttcgtaaact ggttccccgt
    30841 aagacaagaa atactgtttg aagtcattga tcaagtcaac gattgagtca aaaacttctt
    30901 cattttccgc tcgtgccatc aatacttgtt ctgcgccaaa catttctggc acttctgtta
    30961 agatcgtgct gcctccttga gcaatcaagt agtcagaaaa tgcaccaagt aaaggattag
    31021 cagttattcc cgatagacca tcagaaccgc cgcatttcaa accgacattc aacttagaga
    31081 gcgggactga tatgcgctgg tcattttccg ccgctgttaa aagctcttct aatagagcca
    31141 caccttgttc gatctcatcg taaacatctt gagccactag aaatttcact cgttcttcat
    31201 catattcacc taaaattttt ttgaattcag gaactgtatt gttctcacaa cctaatccaa
    31261 atactaaaac tcctccggca ttgggatgat ttactgcgtc cgctaaaatt tctcgagtat
    31321 tttgatgatc ttttccaagc tgcgaacagc cataaggatg ctttaaaatc gtaatatggt
    31381 caaactgtcc cagatctgga tgtttttcct tgaactgttt gacgatcagt tcagcaatgc
    31441 cattcacaca cccaacggta ggtactaaaa acaaatcatt acgaatccct acttttccat
    31501 tttttcgcaa gtaaccttga aatgttcggt tctcaaaaag gtaatgactt ggatggacat
    31561 ctttttcata tcgataatcc aattcaccag aaagattggt ttttacatta tgagtgtgca
    31621 gccattgacc ggcagttacg tctgtcgtca catgaccgat tggatagcca tatttgatca
    31681 cgttgtctcc agctttcaaa tcacccaatg caatcttatg accttgcggg atatcttcaa
    31741 gagtggtgat cgtttgctga tcaatcgtca gttgtgtttg ttttggaata ggggttaatg
    31801 cgacgaccac actgtctcgt cgatttaatt tcattgttgg ttgcagggtt tcaagcatgg
    31861 accatcgttc ctttcatttc ttgtaatact gaccggacac ctttttgatc gatctttgca
    31921 gctatttctt gaactgtatc tgtcacattc ggtatttgag acaaatcttc tccccaaagc
    31981 gcagcgtttt gcaataatcg aggaatagct gttccattat cagaccaagc atcagtaaat
    32041 tcgcttatcg tcttttcatc atcatgtggc tttacttgat cgcctcgata cataagaagt
    32101 agcgcagcta gtgaaacagt gattaacggt ggtacctgat tttgttcttc gatatacttc
    32161 ttgaacactg gaagcagacg ggtgctgaac tttgaaacac tatttaatga aatcgcttct
    32221 aatttgtgat gggcaaaagg attcaagaaa cgttctttga tttggtcagc ataaatggct
    32281 aattcatctt taggtaaaga aagcatagga atcaattcta gattgaataa ctgatcaaca
    32341 tatgcggaga ataaaggatc tttcattact tcttctacag tttccaattc cgccaacaaa
    32401 gcaagaggga ccatcgctgt atgcggtcca ttaagcaaat gcaccttacg ttcacgataa
    32461 ggagtcatat catctgtgat gatgacattc aagccagcct gtttgaatgg tagttgattc
    32521 tccaattctt taggcccttc gatcacccaa agcatgaatg gttctgcttt gaccatcaat
    32581 tgatcttcat agttatgccg ttgattgaaa acacctgcat cttcgcgagg ataacctggg
    32641 acgatgcgat caactaaact acaacagaaa acattttctt tttctaacca ctcaacaaaa
    32701 gcaggttcta aatcccattt ttctgcgtag cgtaaaatga tttcttttag acgttcgccg
    32761 tttctgtcaa tcagttcgca aggaatgatg gtaaagccag ctttgtttaa tttgaaacgt
    32821 cggtataaaa aggctgtcag tttgcccggg aagctctgtg ggggagtatc tgttaaatta
    32881 tcagtctcca cgaattggat tcctgcctct gttgtattgg agacgatata ggccaagtcg
    32941 tcattttctg ctagagatag ataagcatcc cattcatcat aaggattgat catttgttct
    33001 actacggtga taatctcgga agtatcaacg atttcgccat tgctgagtcc ttgtaaaatg
    33061 accgtatata aattatcttg ctggttcatc atatttccaa gaccttgttc taaaggttga
    33121 atgattgcaa cattaccttt aaacaattgt tggttattca gctgctgaat ctgccaatca
    33181 ataaatccac gcataaaatt gccttcacca aactgaagaa cttttacagg tgcttcgttt
    33241 tttttcacta attcttttgt cagttttttc atataaactt cctttctgtg ttatgcacac
    33301 gttaacattt ttatataaaa ttacttgtag tttacaagta attgatcgac tcacgaagga
    33361 tcaacaaggt ggagagctca attttagatt gttcgatcgt cctgccttcg atcaaatcta
    33421 gtaaaagctt tgttccttct tgacacattt ctttcaaagg cttacgtacg gtggtaagcg
    33481 gcggattgag ataggagacg actggagaat catcgaaccc gatcagagaa atgttctggg
    33541 gaacacggag gccagctaaa tgtacagctc tcaatgcacc aatcgctatg tcgtcgttgc
    33601 cgcaaaaaac cagagtaggc aggtcagaaa gtgataatag gtattccatg ttttggaggc
    33661 cgctttctaa gctgtaatct cctgttaaaa aataggctgg attgacaggg atgctcgctt
    33721 tttttagact tttcaatacc cctttttccc gttcttttgt cgaacgaaaa tttttctttc
    33781 cgccaatata agcgatcttc gtatgtccgc atccaatacc gtaatctact gcctgcataa
    33841 caccggccgc atcatcggca gtgacgttga taaccgctgg atcattgatg tgacgattga
    33901 caacaacgaa aggaaattct ttttctttga gatagtcgat gaaaggctga tcatcatcac
    33961 tttgactcat aacgatcagt ccatcgtagc gttgcaggtt gatttctgtt aaatcaacaa
    34021 catcgatact ttcaacagat aaactgtaat ttgtgtctaa tactgaatgg atgcctgcaa
    34081 tggaatctgc taggaaattt gatgaagttc ctttatgcaa acttgagaaa aatagtccga
    34141 ttagatattt tttttgattc actaaacctt ttgcactata gtttggcgaa taattcattt
    34201 ccttagctag ttgtcgtatt ttttgttttg tttcattttt tattaaactg ctgttattca
    34261 aagcacgaga aactgtcgta tgagaaacgc cagctgcttt agcgatatct ttgatcgtta
    34321 ccattccatc attttcctta ctttttcact ttttgttaac gcattcatca tattacgata
    34381 actacgataa gtcaatagat ctttgatatg aaaagattgc acacgttaaa aattacttgg
    34441 atttttattt ttaattatgc tattcttgaa agcggggaat gctcatttaa ttagatgagt
    34501 atcaatttta cagaaaaatg aaagcctttt attttaaaag gaggagagga agaaaatgaa
    34561 gaaaagtaca gtattttata aaatattttt accgatgttt ttattaggag ccgtacttgt
    34621 cataggattt agtttattca tttaccagaa tacctatgaa tcgatcgaat cgagttattt
    34681 aacagacaaa aagaatttgc tcaggcaagt taaaactaac gttgaatgga aaattagaac
    34741 gattgaatat tcgttttcta cgtatggttc gaccaaaaat ttctcggaca tattcaagca
    34801 tccgctgaca tatacagatt actccactta ttctgaaatc aggaaagaac taaattttat
    34861 agaaacgatt gttatggatg ataataatta tgatttagtc agtttggctg gtaaatgggg
    34921 agtaatcaat ggatcgttga gtcaactgac cgaagaggaa gttgaaaagt atcaacagaa
    34981 gtacataaat aacaaaaata atttattttg gcaaaagaaa aacaaaggaa tcgaaatggt
    35041 gatttctttg ccaatgtttg aaaaagaaaa atatgcattg ggaattgcta cgatagagaa
    35101 acatacaata gaacaagttg ttggtgaaag tggagaagac atactttcta ttgaaaacga
    35161 agttggcaac ctttttacta ctcagacagt aaaaaataaa gagcaaccaa ctgtattaac
    35221 ggacgtggat ctagattaca atcagctgaa agtgataaga gaaaaagata aaacgtatat
    35281 agtactgaaa tcagattata ataattggat ttacagatta gaagttgact ctagtgcaat
    35341 tggcgcaatg atccgcaatc tgagaatcgg gctagttgca gtgtctttaa cactggtagg
    35401 actcattggt cttctttcct acttattttc ggacagattt gcccgtccga tttctcaaat
    35461 acaggaacgt ctgaatctta aatcaaaagg attcagtggg aaagaactag atttggtcgc
    35521 tcaatcagtc agtcggatca taggagaaaa agaagcgtta agtgcacact tagttaccca
    35581 aaagccacag ctggaaacgc tatttgtact tagtttattt agaaatcgag tagaaaggcg
    35641 agaactagat cagcggttgc agcaatttgg ttatagttct caaaatgact gctattatac
    35701 agctttggtt caaatcgatc tgttagaaga tagtcacggt ggggagcgtg atttgctact
    35761 tttagcgatc aacaacatga tcacggaaat tgtgccaaag gaaaatcgaa tgctgccgat
    35821 cgttttgaat gaagaaatgc aagccactat ttatcgaatg gataaagatg atgcagaagc
    35881 aagcaaaaaa gtcatggagt attgccgact cattcaaaaa cgtgtaaaag aatacttgaa
    35941 tttaactatc agtataggca tcagtaaccg gttcgaaacg ctgaatgaaa gcaagcaatc
    36001 ggttgatcgg gcaaaagaag ccctgtatta tcgtgtaaat actggtccaa gttcaattat
    36061 tttttatcag gaaatcgttc ctgctgctca tgaaaaaaca cttattagat atccgataga
    36121 agaacaaaat cgattgtttg aagccattcg ttctggaaat caggaagtga agaatttagt
    36181 acatgaattg attgatgcat tgtttgcaca aaataaaaat cctttgagtc gggaagtagt
    36241 ttcggtccgc ttgatcaatg aactcgttca attaggacag ttattgggag tagattcaaa
    36301 aaactttgat aacatgaaac aaatttatgt aaaagcactg aacaattatc atcccaaaga
    36361 actagaaaaa cttgttttag aacaattagt tctgccaatc acagaatgct ctcaagcaat
    36421 cactgatcat gaatttaaaa atttgtcgga gaatattatg catatcgtct acacagaata
    36481 tgatcgtgat ctgtctttag atgtgatcgc tgataggttg cattataacc ctaactattt
    36541 aagtaatgta tttaaaaagg aaacaggaga aaaatttggt gattttgttc aaaatcatcg
    36601 tttagaggta gcaaaaaaat ggttgaaaga gactaacctc tccgtaaaag agattgcaga
    36661 aagattgcaa tatcgaaatc cgcaaaattt tattcgtttc tttaaaaaga aggaatcgat
    36721 tacgccggga gaatatcgaa aaaaatattt gtgaatcaag aggctgtttt tcttgtggac
    36781 tgattcaagc aataaaagaa gttagaataa ccctagtatt tgtatttatg gatagttaaa
    36841 tttcaaggct aagcacgcaa aaagaactgt gacaaaagtc ttgtcacagt tcttttttac
    36901 gaataaatgg cgttcaagag acaggctatc gacaataagg cataattttg tgaaatatgg
    36961 gaagctattt ttcaaaatcg cttcttattg ctcctgtggg ttactcgtcg caatgtttgt
    37021 gagagttttt atcatcgctc aaggctgaac gtctctttca caacctctga tacacggtgt
    37081 tccaggagta gattcttcga aaaataagcc gaaatttcac aaaaatttaa gaagcaattt
    37141 tcgtaaatgc cttcttattt cttgaagctg accacttctg acacaactac cattttgcgt
    37201 tcagtacata atcacccttt gacagaacct acgagtgctc ctttgacaaa atatttctga
    37261 acaaaaggat aaacaatcag cataggcact gttgcaataa cgataacagc cattttaact
    37321 gtttgtgctg gtggaatcac atcaacggca ctactttctg cttgcatccc acttgataca
    37381 atgacgattt ggcgtaaaag aatctgtatc ggccatttag atgaatcgtt gatatacaaa
    37441 atagcagtca tataagtgtt ccagtaagta acagcataaa aaagcgagat agtagcaata
    37501 gacggtaacg ctaatggcag cataattttg aaaaatacac caaaatctgt gcaaccatcc
    37561 atttttgctg attcttccaa actatccggc aaggcttgga aaaagtttct cataatgatc
    37621 atattcattg cattaacagc cacaggcagg atcatggccc agtaagaatt gaccagccct
    37681 agagaacgaa caactaagaa cgttggaatc atccccccgc tgaacaacat agaaaataca
    37741 acaagaaagt tgacgaaacc tcgaccaaac aaatactttc tggaaagacc gtaggccatc
    37801 agtgaagttg cacacatact gacgatcgtt ccgacacctg taacaccaat cgaaacagca
    37861 agtgctctaa aaatggtcgg cgtagaaaga atgtagcgat acgcgtctaa agaaaaggta
    37921 gtaggaaaaa ggataaactt tttcgcgacg acttcttgtg tagaagcaaa agatgtagcg
    37981 atgatattta aaaaaggaac gatacagatc aaagctagca aaattagaaa cgtattgttg
    38041 aaaagtgtaa aacttctttc tcctatagag actttctttt tcatatagat aggctcctcc
    38101 tcaaaataaa ttaaatgttt ctgtttctat atgttcaatt tatcatcggt gaaagattac
    38161 gtatataatc atttcctatg ctaagtgtca gatagaaaaa aatagaaggt ttgctatgcg
    38221 tagtttattt gtgaaaaaat acactttatc gttttgtata gcacaaaaat cattgctatg
    38281 taagcgtttt tacttacggt aaaaatgatt atatacgtaa ttcagatagt tatctatcat
    38341 gaagtcataa gcagcaaaca ttcacaaaac taaggagggg cttatggaaa gtgtaaagaa
    38401 acaagaacca tttcaactga cgctatcgaa aaaagaagaa aagctcagaa gacgaaaaaa
    38461 aataattgca ggaatcaaaa cgaataaatt tttatatgtg atgattctgc cgggagtcct
    38521 ttatttcttg atttttaggt atcttcctat ggggggactc gttattgctt ttcaagatta
    38581 tcaaccattt ttaggaataa tgggaagtcc ttgggtcggt ttcaaacatt tcattcgttt
    38641 gtttacagaa ccgacattct ttacattatt aagaaatacg ttgatcttgt tcggattaaa
    38701 tatcttcgtg ttttttccga tgccaatcat tttgtcttta ctattaaatg aagtaacgaa
    38761 caaacgattg aaaaacggga ttcaaacgat catttacatc cctcacttca tgtcttgggt
    38821 aatcattgtt tcgattactt atgtattttt gaatgttgac ggaggggtat tgaatgaatt
    38881 ccttgcaaca atgggatttg aaaaaatcag ttttctaaca tcccctgaat ggacacgcac
    38941 agtatatatc ggacaagtga tctggaaaga gttgggctgg tctacgatca tctacctttc
    39001 agcgataact gcagtcgata cacagttgta tgaagctgca gaaatggatg gagcaggacg
    39061 cttacgcaaa acatggcatg ttacattgcc ggcaattcgt ccagtaatca ttaccttgct
    39121 gattctaaaa atcggtcaga cattagactt agggttcgag catatgtact tgttattgaa
    39181 ttcattgaat cgaagcgttg cagaaatttt cgacacctat atttatacag ctggattgaa
    39241 gaatggacag ttaagtttca gtacagcaat gggattgttt aaaggggtcg taggattgtt
    39301 tatggtaatt ttagctaata agattgccaa aaagctagga gaagacggcg tttactaaga
    39361 tagaaagaga gggaaagata tgctgcagtc attagataaa ataaacggaa ggctaaatcg
    39421 tattttacag ttggtttatt tgaattttct gtggggcggt ttagtcatcg caggtttggg
    39481 agtttttact ttcggtcctg catcgtatgc gatggttgca gtgattagaa aatggctgcg
    39541 ctcaaaagaa acgtttcctt tgacgaaaac gtacttcaca tattttaaag aaaactatcg
    39601 tgaaaccttg ctgatgagct ggatatatgg cgccatcggg attgttttac ttgtggatct
    39661 ttctttcata cacaattggt atcttaggat cgctttgtat attgctgcgt tcatatatgt
    39721 gctctcagca ctctatattt tcccaatcat ggctcattat gattggaaag gatatttttt
    39781 caaaatgaaa atggcatttt tattcggatt ctcacatctt cattactcat tagtgttgat
    39841 attaagtatg gtagggattt atctgatgat catccaaaca atgccaggtg ttttaacctt
    39901 tatcggtggc agtctcttct tttccgcagt cacgtggaca gcaaattcta tattttgcca
    39961 tttggaatgg aagaactcat atacagaaaa aacagaagaa aacaaaaata ttattaaaac
    40021 agtgaaggag aaaatttatg aaaaaagcaa cgctcgtaaa attaggtctt agttttgcag
    40081 gaattctagc acttgccgca tgcggcagca gttcaagtaa cagttcttca gagacagaag
    40141 atggaaaagc taaaatcact tggttaaata ttttgcatca tgcttcaccg cctacagaca
    40201 cgattgtgaa cttgatcgaa gaaaaaaccg atacggaact gacattcaac tggattcctg
    40261 atgcttcaaa agaagaaaga ttaacaactg cattggcttc tgatgaacta ggagatatcg
    40321 ttacacttca aattgcgatg ttgaaaaact cttctgtccg caatgcgtta aaatcaggcg
    40381 tattctggga tgtatcagaa tatttaaaag actataaaaa tttaagtcaa atttctgaag
    40441 atagaatcga agctgcgagt atcgacggtg ccttgtacgg cgtaccgata caaaaagact
    40501 acgctcgtgc tggcttagtc attcgaaaag actggttgga taatctagga ctagatgttc
    40561 caactaccat ggatgagctt tatgaagtag ctaaacaatt tacagaaaac gatcctgatg
    40621 gaaatggtgt ggacgacaca gttggttttg gcgatcgtgc gcctagtgat attcgttata
    40681 ccagctttaa acttttcact tcttattttg gagcaccaaa tggctgggct gtagacgata
    40741 atggcaaatt cactccagaa tttgatacag aagagtatat gaaagctttg aatttttcaa
    40801 gagatctatt tgagaatggc tatttggcac aagactttgc agttacccaa aagactgatc
    40861 agcaagaaca atttgcacaa ggaagaactg gaatctacac tggaatggtg gatatcaaga
    40921 atctgaagac gatgggtcaa ggtattcaag atgatatgga attagtgcct gtaaacaaga
    40981 tctcagatgg tgaaggcggc ggttatcatg tttggtcaga aggaaatggt attggcggct
    41041 tgctagcatt tcccaaatct caagtaaaag acgaagcaca attgaagaaa ttacttcaat
    41101 ttgttgatga tcttttagaa gaagatgtgt ataaattgat gactggcggt atcgaaggaa
    41161 cgcattatga attgaatgat gatggttcta tcgaatttat cgatatggat ctttggcaac
    41221 aagaggtgca accgttatca agctctcgtc ctagtgaagt gacgttctct ttcaaagata
    41281 cagaccctga aaaagaattc tctaatcaat tgatcaaaga aaatgaagaa tttgctgtaa
    41341 tggatccttc tgtgccattg gattcaccga cgaacaatga agtaggatca gaaattgaaa
    41401 aaatcgtcat tgatgctacg gtgcaataca tcatgggaca gcttgacgaa gatggattca
    41461 aaaaagctgt agaagactgg aaagcacaag gaggcgatcg aatcacacaa gagtacgagg
    41521 aagcatacaa agcagcggca cagtaatata gacgattggg acaaatactt cttggtattt
    41581 gtcccaaatc ctattttaat ttttaaagga gagcaaataa tggaaaactg gtcaaagaaa
    41641 ttagcaaaaa gtatcatgga aagaacacct cgcttatatg aagaaaagtg gtataaagga
    41701 aaatggtctt atgactatgg cgtcgtactt aaagggttcc aattactttg ggaacgaacc
    41761 caagaaaaaa tttattttga tttcataaaa gacaatatcg actatttcgt gcaagaagat
    41821 ggaacgatcc gtggctacag cgttgaagaa tacaatatcg accatgtaaa tacaggaaag
    41881 cttttctttt tattgtacaa agaaacaggc gaagaaaaat ataaaaaagc agcagaactc
    41941 ctttcccagc agttggcaaa ccatccacgc acatccgaag gagctttttg gcataaagaa
    42001 atttatcctt atcaaatttg gttggatggc ttatatatgg gttctccttt ttatgctgaa
    42061 tttatccaga attttagtga agaaaaagat taccaagatg ttttgagaca gtttgaaatc
    42121 agttataaac atctattcga tccaaaaaca aatttgctga ttcatgcatg ggatgaaaag
    42181 aaggtccaac catgggctga tccaaaaaca ggtctatccc atcatttttg gagccgatcg
    42241 attggctggt atttgatggc tgcagcagat acctatgaat tacttcatga agaaacagat
    42301 tgttctttgc tgaaagaaat tctagaaaag acactggctg cattacttcc atatcaagaa
    42361 gaaaacagcg ggacatggta tcaagtaaca acagaaacaa aacggaaagg aaattatctg
    42421 gaagcttccg gcagtagtat gtttgtttac gcaatggcta aaggaatacg tttgggactg
    42481 ctgaacaaag aagaatggct tccacaagtg aagaaatcac atcaaggatt acttgatgaa
    42541 tttgttctgg aaacaaaaga aggctggctg aacttgaata aaaactgtca agtagcaggg
    42601 ttaggcggag cagatcagcg agatggttca tatgcttact acatcagtga accaattatt
    42661 tgtaatgatc aaaaaggcgt tggtgcattc ttgcaagcat tgattgaagt ggaggcatta
    42721 taaatatgta tgatattttg acatttggcg catcaattga tgaattgaat actgaagcaa
    42781 tacaacaagc cattgatgct gcagcaagtg acggaggcgg gaccgtagtt gttccagcag
    42841 gtgaatttct aacaggagct ttatttttaa aaagcaatgt cgaacttcat ttaagcgcag
    42901 gagcggttct gaaattctct gacgatccaa aagattatcc tgttgttcat tcacgttggg
    42961 aaggagtcca tcgaaaagtt tatgcttcct gtatctatgc tcaaaatgta gagaacatct
    43021 ctgtcactgg tttcggtaca ttagatggaa atggaaaaaa atggtggcat acttttagaa
    43081 atgaaccaga taatctcgct tatcctcgtc ctaaattgat gagtttccat aactgtcatc
    43141 gaattactgt gaaagatatt aaattgatcc agtcaccaag ttggacgatc aacccgattc
    43201 tttgcagtaa tgcaacgttt gataacttga cgatcttgaa cccagcggat tcaccaaata
    43261 cagacggaat tgatccagaa tcatgcaaaa atgtgcgtat tagtaattgc catattgacg
    43321 tgggagatga ctgtattgca atcaaggcag ggactgaaga tacgtacgaa aggatcgctt
    43381 gcgaaaatat cacgatcaca aattgcacga tggtccatgg acatggcggt gtcgtattag
    43441 gaagtgaaat gagtggtagt attcgtaata ttaccatttc aaattgtatc ttccaagaaa
    43501 ctgatcgagg aatacgattg aaatctagac gaggtcgagg cgggatcgtt gaagatattc
    43561 gagtatccaa tatagtcatg gataatgtga tgtgtccatt tatactgaat ctttattatt
    43621 tctgtggtcc gcgagggaaa gaaccgtatg tatgggaaaa aaaggcttat ccgatagatg
    43681 aacggacacc agcattcaga aggatccatt tttcgaatat cacggcaagg aatgtccatg
    43741 cttctgcagg ttttatttat ggattagcgg aacaattcat acaagaaatc acatttgaca
    43801 atatcgatgt ctcgatggcc caagatgcag ttcccggtgt tcctgctatg atgacaggaa
    43861 tagaggaaat gagcagacaa ggattttata ttggctttgc gggtaaggtt tcctttaacc
    43921 agatgtcgat tgaaaatcac gagggaccag catttctatt agaaaatact aaagaaatca
    43981 gtattgagaa attccaatca aatcaatcag aaaatatgga tgaactggtt gttcgtaaaa
    44041 actttgctta tgcagttgaa tgatttttta ggtgctccat ctagcagaca tacccctagt
    44101 ggaaaacgat tataccgaga aaaacaaaac ggttttatgc ttgaatacta tcaattgtac
    44161 ttaaatgaat tggaaacagt accagccctc tatgcttatc cagaaaaaga aggaccgttt
    44221 ccgccggtcc tttacctgca ttctcacggc ggagattttt cagtcgggaa aagtgaattg
    44281 atccatggtg ctccttactt agcttctcca tcttttgcag aagtactgac aggaatggga
    44341 tatgctgttt ggtcgattga tgcttgggga tttgaagaac gcggaggtat ttctgaaagc
    44401 gagttgttca aacaattttt attaacagga aaaacgttat ggggaatgcg tatttatgat
    44461 gtgatcagct tgatcgatta tttggagaca agggaagaca cagatactca acggctcgca
    44521 acgattggtc tttctatggg aggcttatta agctggtggg tgacagcatt ggattctcgt
    44581 gtcaaagttt gtattgatct agcggcacag gtggacatcg aaatactttt gctacatcgc
    44641 cgcctagatc atcatgggtt ttattactat gtgccaaatc tattgacagc ttatacaacg
    44701 ttagcaatcc aagaaaaaat cgctccgcgc cctcgtctta gcttagttgg aaaaaacgat
    44761 acgatgtgct tggacgaagg cgtactgaag ctgagaaaag ggctggacaa aaagtatcaa
    44821 tcaatgggta gtccagaaaa cttttcttct ttttctttaa caggcggtca tatggaaaca
    44881 caagaaatga gaaatatttg gaaacaattc ataagggagc atttatgagg atgaaagaca
    44941 aactgataat tggaaagaat gctatagata ccccgacgga ttttgaaact atccaagaag
    45001 gaatcgatta tttggcaggc ctgccggata gccatccaaa ggtcttgata gtaaaagaag
    45061 gaagatatga tgaatatata gaaagcagat tatccaattt taaacttgtg ggagcaggaa
    45121 cagtagttat ctccggtaat cgttttgcaa aacaggagta tcttgacggt agccagttag
    45181 ggactttccg aagtgctact tttttcttag aaggatcgaa tgtcgtatta gaaaatcttc
    45241 acatcgaaaa tacagcaggt ccaggagaaa aggtaggaca ggctgttgct ttattcaatt
    45301 caggagatcg cgtatcttta aaaaactgtc gactatctgg ttatcaggat accttatgca
    45361 cgggtccgtt gcctgatttg caaaaagatg gtactccttt ttgtacaccg gcttctgcca
    45421 atcctgagta ttgtcgtcag acctatgaat attgctggat tgaaggaacg gtggatttca
    45481 tatttggtgg ggcagatgcc gtttttcatc agtgtcatat ccatagctta gccagtaaaa
    45541 atcctggata catcacagca gcatctacgc cgaaaaatca aaaaacaggg tatcgttttg
    45601 aaagctgtac cttgactgca gaacctgata ctagaaacgt ctatttagga agaccgtggc
    45661 gtccgtatgc aaatgtctta tttgaagcgt gtaagatagg tgggcatgtt cacccaactg
    45721 gttgggataa ttgggatgac aagaaaaatg aacagactgt caaattctct gaacgtaata
    45781 acaattatga aacatcgatc aaaagacctg aatggataca tttagaaaag cagggggttc
    45841 agaaatgaaa agaaaatggg aactattgct aggaatggtc ggtggcagtc tatctttgat
    45901 tttctttggc gggttagcag ttactttaag taatatgtct gcgtcagagt ttaaaaaaag
    45961 ttatcagtcg cttgctgtag atcattcaac tttgtcttta gaaaatacat ttggattatt
    46021 acaggacatg actggtctgt ttgccgttgt gttgtttatt tcacttgctt ttttagcagt
    46081 agcattgttt ttgactgcta aaggaaaata cttaacaact gccactggtt tgtattttat
    46141 aacaggattc atattgttga ttggcactca gtttattgcg tttccttttg cattttttta
    46201 tttcgcagct ggggcattca gtttatatcg agtaagaatg agaaaaggag cctgaatcat
    46261 gcagactatc aaagtgaaaa atcctatttt aagaggattc aatccagatc ctaatatatt
    46321 aagagtgaaa gagcggtatt acattattgt ctcctctttt gaatggcttc caggaatccg
    46381 agtatatgag tcgaaaaatt tagtcaactg gtcacataca acggatattt tgaccactca
    46441 agtatctttg caaggaaatc ctacaaatgg aagtatatgg gcgccacaaa tcagttatgc
    46501 tgatggattg ttttatcttg tttacacaga tgtcaaaagt gcaggccgtc cgtttaaaga
    46561 tgtccacaat tatttgatca ctgcatctga gatcacaggt ccttggtccg aaccaattta
    46621 tttaaatagt aacggctttg atccttcttt gttccatgat cttgaaacgg gaaaaaaatg
    46681 gcttgtcaat gaactatggg attatcgtct ggacacaccg aataaatcca gcggtatcgt
    46741 attacaagaa tatgatgcag agaagaagaa gcttgatggt cccatctata aaatatttgc
    46801 aggtacagaa ttagcgaaaa cggaggcacc acatttgtat tttcgtaacg ggtattatta
    46861 tttgatgacg gcagaaggcg gtacaggaag aggacattca gttacagttt gccgagcaaa
    46921 gaatattact ggtccttacg agcttgatcc tggttttcct atgctgacag ctagtgacaa
    46981 acctgcttct acattgcaat gtacaggaca tgggagctta gtacaaaccc cgtcaggaag
    47041 atggtacatg acatatcttt gtaccagacc attaaacggt gcggctattt tgggaagaga
    47101 aaccgctatt caagaagtgt attggacgtc cgacgattgg ttaagactta aagatagcca
    47161 gacaactccg atggaagaaa taatgattga gacaaatgaa gaggtacaac aagaaaaaga
    47221 tcatcagttt atggatacat ttgaaggaga gctaaagaag aactggaatg ccagacgcat
    47281 tttgccaaat gctgattggt gtgatacaaa atgtagacca ggttttttac gtctgatttc
    47341 cggtgaatct cttcaatcta ccttcgaaca tcatttgtta gctattcggc aaacggattt
    47401 tacttttgat gcggagacag catttgaata catgcctaat aattttaatc agatggctgg
    47461 actggtactg tacttaaatg aatccaatta tatttattgt tatgttactt ggaatgagga
    47521 caagggaaaa tgcctacgta tgatagaatc agaaaacgga gtagcacaaa tagaagattg
    47581 gacgattcca ctcacagaaa agaagacttt tttgaaagtc actgtaaaga aagagacagc
    47641 acagtttcat ttgaaagacg tatcaactga agaatggaag aaagtaggtc cgcaaaaaaa
    47701 catgtgtata ctctcaggag ggtttactgg aaattttatc gggatagcag tgcatgatct
    47761 aaataaaaaa agaggaagtt atgctgattt tgagtttttc aagtatgatg gaaaagatca
    47821 tctgtaattt aattctttaa taaatgaggt tcactaactt attttcacta gttagtgaat
    47881 ttttttgttt gtatatattg ttatgtaaaa aaagttatca atcttatgca tcaatgtttt
    47941 tagattattt tttgtttttt atctgtcaag ataatgcaat aaaaagccaa cattgtgaat
    48001 ataaaaacaa gaaaaaaaga gttataattt tgaaaacgtt tactagaaga ggtaggagga
    48061 attcaaatgc tttcacaaaa tgctgttaca gcaaatacaa taaaaaaaga aaaaaaggaa
    48121 tttgggattt ccgataagat aggctacatg ttcggtgatc taggtaactg ttttatttta
    48181 ggtttagtca atagtttctt gatgatttat tatacgaatg ttcttggcgt ttcaggggca
    48241 atcgtaggta cgttattctt gatttcgaga attttggatg cgtttgctga tgtgatggtt
    48301 ggaagattat gtgatgtctc agagttaaca gaaactggcc gatttattcc ttggatcaga
    48361 aaaatgaagt atccattttg cttagtgact gtcattttgt ttttgccatt cgtccaacat
    48421 cttccaatga cggtgaagat tgcttatatt tttgtttcat acattgttta tgggattttt
    48481 ctctcaacaa tcaatatccc gtatggagca atggcagcag caattagctc aaatcctaat
    48541 gaccgcacct cgttgtccac gttccgaagc attggttctg cgattggcgg atcaactaca
    48601 ggattcttca ttccgatttt aatgtatacc cataacaaga gtggacaaca agtcgtttca
    48661 gggaatcatt tcttttggat ttcaatcgga tgtgctgcaa tagcatttgt cgcatatatg
    48721 ttaacttgta aattgactac tgaaagagta agagttgtca aaaacgaaaa agtatctgct
    48781 tctcagttgg taaaaggttt gttagctaat cgagcactga ttattttagt cattgttgac
    48841 attttcattg tgatcaatca gattttagct gggacgaata tgacttattt attcaatgat
    48901 tacttccata ataaacaagc aatgtctgtt gctcttttat ttacttacgg aacagtagta
    48961 gttttagcac catttgctac aaagctgaca aaattatttg gtaaaaagga agcaagcgta
    49021 tttgcgttac ttgcttcttc atgtatgtac ttgctgatgt atttccttca tataacaaat
    49081 gcatgggtat atctgattct tttatttatc gctactttag gatctggttt attcaatttg
    49141 atggtctggg cattcattac tgacgtgatt gattatcatc aatataagac aggcttaaga
    49201 gaagatggaa cagtatatgg agtaaattct tttgcgagaa aagtaggaca agcatgtgca
    49261 ggagcggtag gcggatttat gctggcactc attggttacc aatcgtctac aacaggtgga
    49321 gccatccaat cagttttagt acaagaaagg atttatacga tggcaaactt gttaccagca
    49381 ttctgcttat ttgtatccgc aatcattttg ctagtaggtt acccactgaa tcgttcaaaa
    49441 actgtattta ttggtgaaga attgaaaaaa atcaatgaaa gatagggaga aacgtattgc
    49501 ggacaaaaaa agggattatc aatgctttga actttaaaac aaacgacact caacaagatg
    49561 atacaataat actgcaaaaa gcaatcgatc aaggtgcgaa agaacactta cctgtattta
    49621 ttccaaaagg catttattta gtcggagccc tttttttaaa agataagagt catcttatct
    49681 ttgaagaagg tgcagtcctc aaggggagaa cagatatcga agcatttcca gaaatcgata
    49741 cacgagtagc cggcgtagaa atgaaatggc cagcagcgat tttgaatgtt ctctcggcga
    49801 aagatattct tatagaagga aaaggaataa tcgacggtca aggagaccat tggtgggagc
    49861 tgtattgggg aaaagatcaa aaaagcggga caagagctga atacgatcaa aagggacttc
    49921 gttggattgc ggattatgct attaaacgtc cacgcgcttg tttactgtat catgcagaac
    49981 atgttgttgt tcgtgatttg atttttcaga aatcaggttt ttggaacttg caaatcactt
    50041 attctaacga tgtattagta gaaaaagtaa tcatccgcaa caatgatgga ccaagtacag
    50101 atgggattga tattgattct tcaacaaatg tgcgtgtcta tgaatgtgat ttggcctgtg
    50161 gtgatgattg tattgctatt aagtctggca gagatggcaa tggtgcaaga gtcaatcgaa
    50221 aatcatcaag aatcgaggtt gctagatgta aaatccgttc aggatacggt gtaactattg
    50281 gtagcgaagt tagtgctgga gttagtgacg tatatattca cgatatcgat ttttttcagt
    50341 cagactgtgg tattcgaatg aaatcttcaa aagaaagagg gggagtgatc gaaaatatca
    50401 gggtagaaaa tctgaatatg attgatgtac aatttccttt ttcttggata atggactggc
    50461 ataatgaata caatagaaaa aatagcaatg accttgctca aatgccagaa agttggcaag
    50521 cagtggctga agaaatacct gaatcaaagc aaatgacgaa agtcaaagac atacacatca
    50581 aaaacgtaca ggcatgcctt tctccaggtt actcacttcc ttcccgagct tttgatttag
    50641 tagcatttcc tgaaaagcct atagaagatg tctgcttttc tcattgccaa ataactgcaa
    50701 aagaattcgg gcgaatcgaa gcagtacaaa acttatgttt caaaagctgt gatttatcta
    50761 tagaaactgg caacacagct gcaaacaata cttttgacaa tcgttgaaat tataataagg
    50821 tcaagcttaa ataatatttt agcttgacct ttattttttt taatgatata ttaaacaacg
    50881 ttgtcgcgct tcattgaaac attttatgaa aattccaaaa aagagttgac acatgttttt
    50941 agacgtgata agataactaa gtcgt
//
LOCUS       DASJSZ010000009        50922 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.009, whole genome shotgun sequence.
ACCESSION   DASJSZ010000009 DASJSZ010000000
VERSION     DASJSZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.009"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            137..508
                     /locus_tag="U4B03_000489"
     CDS             137..508
                     /locus_tag="U4B03_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN2067366.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(782..1474)
                     /locus_tag="U4B03_000490"
     CDS             complement(782..1474)
                     /locus_tag="U4B03_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2067367.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1477..2262)
                     /locus_tag="U4B03_000491"
     CDS             complement(1477..2262)
                     /locus_tag="U4B03_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2067368.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2499..3899
                     /gene="lacG"
                     /locus_tag="U4B03_000492"
     CDS             2499..3899
                     /gene="lacG"
                     /locus_tag="U4B03_000492"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN2067369.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3924..4241
                     /locus_tag="U4B03_000493"
     CDS             3924..4241
                     /locus_tag="U4B03_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2067370.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4254..5927
                     /locus_tag="U4B03_000494"
     CDS             4254..5927
                     /locus_tag="U4B03_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEN2067371.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5941..7572
                     /locus_tag="U4B03_000495"
     CDS             5941..7572
                     /locus_tag="U4B03_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEN2067372.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8063..9628)
                     /gene="guaA"
                     /locus_tag="U4B03_000496"
     CDS             complement(8063..9628)
                     /gene="guaA"
                     /locus_tag="U4B03_000496"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN2067373.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9687..10610)
                     /locus_tag="U4B03_000497"
     CDS             complement(9687..10610)
                     /locus_tag="U4B03_000497"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEN2067374.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10806..11411
                     /locus_tag="U4B03_000498"
     CDS             10806..11411
                     /locus_tag="U4B03_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2067375.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11719..12735
                     /locus_tag="U4B03_000499"
     CDS             11719..12735
                     /locus_tag="U4B03_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2067376.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12876..14177
                     /locus_tag="U4B03_000500"
     CDS             12876..14177
                     /locus_tag="U4B03_000500"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEN2067377.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14223..14885
                     /gene="deoC"
                     /locus_tag="U4B03_000501"
     CDS             14223..14885
                     /gene="deoC"
                     /locus_tag="U4B03_000501"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN2067378.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14906..15304
                     /locus_tag="U4B03_000502"
     CDS             14906..15304
                     /locus_tag="U4B03_000502"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN2067379.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15370..16452
                     /locus_tag="U4B03_000503"
     CDS             15370..16452
                     /locus_tag="U4B03_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2067380.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16664..18226
                     /locus_tag="U4B03_000504"
     CDS             16664..18226
                     /locus_tag="U4B03_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2067381.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18219..19352
                     /locus_tag="U4B03_000505"
     CDS             18219..19352
                     /locus_tag="U4B03_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2067382.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19352..20308
                     /locus_tag="U4B03_000506"
     CDS             19352..20308
                     /locus_tag="U4B03_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2067383.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20407..21573
                     /gene="deoB"
                     /locus_tag="U4B03_000507"
     CDS             20407..21573
                     /gene="deoB"
                     /locus_tag="U4B03_000507"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN2067384.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21597..22415
                     /locus_tag="U4B03_000508"
     CDS             21597..22415
                     /locus_tag="U4B03_000508"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN2067385.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22432..23139
                     /gene="deoD"
                     /locus_tag="U4B03_000509"
     CDS             22432..23139
                     /gene="deoD"
                     /locus_tag="U4B03_000509"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN2067386.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23437..24123)
                     /gene="gpmA"
                     /locus_tag="U4B03_000510"
     CDS             complement(23437..24123)
                     /gene="gpmA"
                     /locus_tag="U4B03_000510"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN2067387.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24295..24876)
                     /locus_tag="U4B03_000511"
     CDS             complement(24295..24876)
                     /locus_tag="U4B03_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2067388.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25009..25692)
                     /gene="rpiA"
                     /locus_tag="U4B03_000512"
     CDS             complement(25009..25692)
                     /gene="rpiA"
                     /locus_tag="U4B03_000512"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEN2067389.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26011..26424
                     /gene="rpsL"
                     /locus_tag="U4B03_000513"
     CDS             26011..26424
                     /gene="rpsL"
                     /locus_tag="U4B03_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEN2067390.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26508..26978
                     /gene="rpsG"
                     /locus_tag="U4B03_000514"
     CDS             26508..26978
                     /gene="rpsG"
                     /locus_tag="U4B03_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEN2067391.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27072..29159
                     /gene="fusA"
                     /locus_tag="U4B03_000515"
     CDS             27072..29159
                     /gene="fusA"
                     /locus_tag="U4B03_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN2067392.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29331..30518
                     /gene="tuf"
                     /locus_tag="U4B03_000516"
     CDS             29331..30518
                     /gene="tuf"
                     /locus_tag="U4B03_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN2067393.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30925..31233
                     /gene="rpsJ"
                     /locus_tag="U4B03_000517"
     CDS             30925..31233
                     /gene="rpsJ"
                     /locus_tag="U4B03_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEN2067394.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31264..31893
                     /gene="rplC"
                     /locus_tag="U4B03_000518"
     CDS             31264..31893
                     /gene="rplC"
                     /locus_tag="U4B03_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEN2067395.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31921..32544
                     /gene="rplD"
                     /locus_tag="U4B03_000519"
     CDS             31921..32544
                     /gene="rplD"
                     /locus_tag="U4B03_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEN2067396.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32544..32834
                     /gene="rplW"
                     /locus_tag="U4B03_000520"
     CDS             32544..32834
                     /gene="rplW"
                     /locus_tag="U4B03_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEN2067397.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32873..33706
                     /gene="rplB"
                     /locus_tag="U4B03_000521"
     CDS             32873..33706
                     /gene="rplB"
                     /locus_tag="U4B03_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEN2067398.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33749..34027
                     /gene="rpsS"
                     /locus_tag="U4B03_000522"
     CDS             33749..34027
                     /gene="rpsS"
                     /locus_tag="U4B03_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEN2067399.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34050..34397
                     /gene="rplV"
                     /locus_tag="U4B03_000523"
     CDS             34050..34397
                     /gene="rplV"
                     /locus_tag="U4B03_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEN2067400.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34411..35067
                     /gene="rpsC"
                     /locus_tag="U4B03_000524"
     CDS             34411..35067
                     /gene="rpsC"
                     /locus_tag="U4B03_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEN2067401.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35070..35504
                     /gene="rplP"
                     /locus_tag="U4B03_000525"
     CDS             35070..35504
                     /gene="rplP"
                     /locus_tag="U4B03_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEN2067402.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35494..35682
                     /gene="rpmC"
                     /locus_tag="U4B03_000526"
     CDS             35494..35682
                     /gene="rpmC"
                     /locus_tag="U4B03_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEN2067403.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35707..35973
                     /gene="rpsQ"
                     /locus_tag="U4B03_000527"
     CDS             35707..35973
                     /gene="rpsQ"
                     /locus_tag="U4B03_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEN2067404.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36031..36399
                     /gene="rplN"
                     /locus_tag="U4B03_000528"
     CDS             36031..36399
                     /gene="rplN"
                     /locus_tag="U4B03_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEN2067405.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36436..36744
                     /gene="rplX"
                     /locus_tag="U4B03_000529"
     CDS             36436..36744
                     /gene="rplX"
                     /locus_tag="U4B03_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEN2067406.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36772..37311
                     /gene="rplE"
                     /locus_tag="U4B03_000530"
     CDS             36772..37311
                     /gene="rplE"
                     /locus_tag="U4B03_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEN2067407.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37330..37515
                     /locus_tag="U4B03_000531"
     CDS             37330..37515
                     /locus_tag="U4B03_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEN2067408.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37551..37949
                     /gene="rpsH"
                     /locus_tag="U4B03_000532"
     CDS             37551..37949
                     /gene="rpsH"
                     /locus_tag="U4B03_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEN2067409.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37981..38517
                     /gene="rplF"
                     /locus_tag="U4B03_000533"
     CDS             37981..38517
                     /gene="rplF"
                     /locus_tag="U4B03_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEN2067410.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38678..39034
                     /gene="rplR"
                     /locus_tag="U4B03_000534"
     CDS             38678..39034
                     /gene="rplR"
                     /locus_tag="U4B03_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEN2067411.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39055..39555
                     /gene="rpsE"
                     /locus_tag="U4B03_000535"
     CDS             39055..39555
                     /gene="rpsE"
                     /locus_tag="U4B03_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEN2067412.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39570..39749
                     /gene="rpmD"
                     /locus_tag="U4B03_000536"
     CDS             39570..39749
                     /gene="rpmD"
                     /locus_tag="U4B03_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEN2067413.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39793..40233
                     /gene="rplO"
                     /locus_tag="U4B03_000537"
     CDS             39793..40233
                     /gene="rplO"
                     /locus_tag="U4B03_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEN2067414.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40233..41528
                     /gene="secY"
                     /locus_tag="U4B03_000538"
     CDS             40233..41528
                     /gene="secY"
                     /locus_tag="U4B03_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEN2067415.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41590..42237
                     /locus_tag="U4B03_000539"
     CDS             41590..42237
                     /locus_tag="U4B03_000539"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEN2067416.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42428..42646
                     /gene="infA"
                     /locus_tag="U4B03_000540"
     CDS             42428..42646
                     /gene="infA"
                     /locus_tag="U4B03_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEN2067417.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42679..42795
                     /gene="rpmJ"
                     /locus_tag="U4B03_000541"
     CDS             42679..42795
                     /gene="rpmJ"
                     /locus_tag="U4B03_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEN2067418.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42813..43178
                     /gene="rpsM"
                     /locus_tag="U4B03_000542"
     CDS             42813..43178
                     /gene="rpsM"
                     /locus_tag="U4B03_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEN2067419.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43206..43595
                     /gene="rpsK"
                     /locus_tag="U4B03_000543"
     CDS             43206..43595
                     /gene="rpsK"
                     /locus_tag="U4B03_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEN2067420.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43675..44613
                     /locus_tag="U4B03_000544"
     CDS             43675..44613
                     /locus_tag="U4B03_000544"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEN2067421.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44641..45021
                     /gene="rplQ"
                     /locus_tag="U4B03_000545"
     CDS             44641..45021
                     /gene="rplQ"
                     /locus_tag="U4B03_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEN2067422.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45293..46132
                     /locus_tag="U4B03_000546"
     CDS             45293..46132
                     /locus_tag="U4B03_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2067423.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46108..46977
                     /locus_tag="U4B03_000547"
     CDS             46108..46977
                     /locus_tag="U4B03_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2067424.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46967..47764
                     /locus_tag="U4B03_000548"
     CDS             46967..47764
                     /locus_tag="U4B03_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN2067425.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47967..48713
                     /gene="truA"
                     /locus_tag="U4B03_000549"
     CDS             47967..48713
                     /gene="truA"
                     /locus_tag="U4B03_000549"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN2067426.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            49378..49615
                     /locus_tag="U4B03_000550"
                     /pseudo
     CDS             49378..49615
                     /locus_tag="U4B03_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(49759..50535)
                     /locus_tag="U4B03_000551"
     CDS             complement(49759..50535)
                     /locus_tag="U4B03_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2067427.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            50658..>50922
                     /locus_tag="U4B03_000552"
     CDS             50658..>50922
                     /locus_tag="U4B03_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN2067428.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHE"
BASE COUNT        16153 a         9271 c        11052 g        14446 t
ORIGIN      
        1 cactcagaga acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc
       61 tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga
      121 gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca
      181 atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc
      241 ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa
      301 aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt
      361 taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga
      421 aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg
      481 tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca
      541 aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt
      601 taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa
      661 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
      721 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
      781 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
      841 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
      901 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
      961 cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa
     1021 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
     1081 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
     1141 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
     1201 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
     1261 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
     1321 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
     1381 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
     1441 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
     1501 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
     1561 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
     1621 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
     1681 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
     1741 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
     1801 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
     1861 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
     1921 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
     1981 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
     2041 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
     2101 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
     2161 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
     2221 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
     2281 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
     2341 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
     2401 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
     2461 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
     2521 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
     2581 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
     2641 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
     2701 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
     2761 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
     2821 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
     2881 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
     2941 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
     3001 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc
     3061 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
     3121 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
     3181 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
     3241 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
     3301 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
     3361 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
     3421 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
     3481 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
     3541 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
     3601 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
     3661 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
     3721 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
     3781 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
     3841 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
     3901 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
     3961 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
     4021 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
     4081 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
     4141 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
     4201 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
     4261 gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt
     4321 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
     4381 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
     4441 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
     4501 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
     4561 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
     4621 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
     4681 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
     4741 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
     4801 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
     4861 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
     4921 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
     4981 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
     5041 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
     5101 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
     5161 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
     5221 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
     5281 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
     5341 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
     5401 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
     5461 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
     5521 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
     5581 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
     5641 ctggcgcagg gacaagtggg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
     5701 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
     5761 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
     5821 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
     5881 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
     5941 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
     6001 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
     6061 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
     6121 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
     6181 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
     6241 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
     6301 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
     6361 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
     6421 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
     6481 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
     6541 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
     6601 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
     6661 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
     6721 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
     6781 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
     6841 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
     6901 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
     6961 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
     7021 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
     7081 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
     7141 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
     7201 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
     7261 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
     7321 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
     7381 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
     7441 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
     7501 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
     7561 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
     7621 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
     7681 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
     7741 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
     7801 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
     7861 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
     7921 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
     7981 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
     8041 ccctccatat tatgttggct ctttattccc actccactgt tgctggtggt ttagaagtga
     8101 tgtcatacac gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt
     8161 gcaagacttc ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag
     8221 cacggatacc gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga
     8281 tccctggaag aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt
     8341 cttcacgcaa gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc
     8401 caagaacacg aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag
     8461 gcatgcctaa ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt
     8521 cgatcaattc aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg
     8581 tctgtgctgt ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga
     8641 aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt
     8701 cattcccaat gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga
     8761 agcgatctct tgcattgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca
     8821 ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt
     8881 ggtcaccgat cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa
     8941 gaccaagcaa gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga
     9001 taaagttatc cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca
     9061 gcaattcgat tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat
     9121 tacgtgttgt gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa
     9181 agccttcagg aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg
     9241 gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt
     9301 cgcggttttt agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat
     9361 agcaaatccc taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat
     9421 tctcatcata tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt
     9481 ttacttcttc tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa
     9541 tacgtcttgt gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa
     9601 cgttcgtcat atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct
     9661 caaaatagtc aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata
     9721 tgattttcag ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc
     9781 tgtaaatttt tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca
     9841 cgatctccga tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt
     9901 aacgcaccaa aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag
     9961 acagaaaaat caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta
    10021 ttgatcccct caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg
    10081 atatcataca catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca
    10141 cggttcaaga aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc
    10201 atgattccac gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc
    10261 tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta
    10321 ctttttccaa cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat
    10381 ttatgaagaa ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa
    10441 atcagatgag caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc
    10501 aaacttttga tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat
    10561 tctcgccatt cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc
    10621 tgcgttctcc catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa
    10681 gagagattgc cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt
    10741 gacttgcgca cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg
    10801 aagacatgac aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat
    10861 gggcttttga gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac
    10921 gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac
    10981 ctgatgaagg aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg
    11041 catttgcgac acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag
    11101 cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct
    11161 atgaagcagt tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg
    11221 gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta
    11281 cactgacaat tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag
    11341 aaacatttgg taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg
    11401 tgaaagaata actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg
    11461 tacgggtacg tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt
    11521 tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata
    11581 cagaacttgt atagaaggct gccaagtcgg tagataaagg aggtttttat caaaaaacat
    11641 actttccttt tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga
    11701 tagaagggag ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta
    11761 tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca
    11821 agaaaaaaag tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca
    11881 aatgtgatca atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc
    11941 ttcttctcca aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc
    12001 atgttatgca attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc
    12061 caccgctcag tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca
    12121 ttagacagcg gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt
    12181 cgggataacg gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg
    12241 tttattgaaa atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa
    12301 ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat
    12361 gggaaatttg ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa
    12421 ttactcaaac ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga
    12481 tgctatcagg cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga
    12541 ttcgatggat taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca
    12601 agttttgata ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa
    12661 agaaaagttc cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta
    12721 ttgacaaagg gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag
    12781 tgcttttttt atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat
    12841 gtataatacc gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa
    12901 aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata
    12961 cgaatggaaa gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag
    13021 acatgacaaa tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca
    13081 tcgatttgag ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg
    13141 acacgaccac attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa
    13201 tgtctggacg cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg
    13261 gatttcagat cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag
    13321 cggtaattgg tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg
    13381 acgtcacagc gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa
    13441 tcgcggcagg agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga
    13501 agaacatcga agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta
    13561 accgagaaac agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa
    13621 atagtctaga agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag
    13681 tagaaatgtg ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa
    13741 ccgatgaagc aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat
    13801 tcaaagaaat gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa
    13861 ttttgactgc acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa
    13921 tcgtggcaaa tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag
    13981 aagaagatat tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga
    14041 caaaaggtga gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg
    14101 aattattata taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg
    14161 acatcatcac agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag
    14221 aaatggaact aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag
    14281 ctgtgatgca aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc
    14341 cagcatgggt tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag
    14401 taatcgggtt tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg
    14461 ccatcaataa cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa
    14521 aggatgatca acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg
    14581 cgcttgtcaa agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt
    14641 gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag
    14701 gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg
    14761 tcaaagcttc cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg
    14821 ctacaagaat cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct
    14881 attaattttt tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc
    14941 tattgaagcg ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct
    15001 cgtaacggaa tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt
    15061 aacgaattgt gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt
    15121 tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg
    15181 acaagtaatg gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg
    15241 tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct
    15301 ataactcatg ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc
    15361 tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg
    15421 ttatttttag gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca
    15481 tcagacacaa caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc
    15541 aaccagtcag cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga
    15601 aatgatggct tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac
    15661 caagcattga atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg
    15721 atccaagaac aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc
    15781 gatggattag ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca
    15841 ggtgttgcag cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa
    15901 ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa
    15961 gaattaggaa aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat
    16021 aaaggacgtt caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct
    16081 tcaggcggta caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac
    16141 aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta
    16201 aacggcgaaa agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta
    16261 gaagatttgg cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat
    16321 ggattgaaag aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa
    16381 gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct
    16441 gaagaaaact aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt
    16501 tgattagaac aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa
    16561 caactccctg aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt
    16621 caatcgttgc cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat
    16681 tgtcatagaa atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat
    16741 caatttacaa gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa
    16801 atctacattg atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat
    16861 gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat
    16921 ggtccatcag cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg
    16981 aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa
    17041 agtttctgaa cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt
    17101 tggaatggaa caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat
    17161 ttttgatgag ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat
    17221 gaaggaatta gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat
    17281 caaagcagta gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa
    17341 cgttaaagac gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt
    17401 caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt
    17461 ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg
    17521 cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt
    17581 gactggtttg cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa
    17641 taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa
    17701 atacgggtta gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca
    17761 aaaaccttac agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga
    17821 attgatcgaa gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc
    17881 aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt
    17941 gattgttgca aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg
    18001 tctgatcgaa caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga
    18061 aattttaaat gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt
    18121 tgatccgaaa gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga
    18181 agaagcacga aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat
    18241 tccgtaattt attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat
    18301 tgatggctgc ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc
    18361 ttggttcgca aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag
    18421 cattaggttt ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag
    18481 cactttgcgg gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag
    18541 ttgttttgct tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg
    18601 ttccaggttt acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga
    18661 actatatctt attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc
    18721 gctcaagttt ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt
    18781 taacacaagc ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc
    18841 ttattttcgt ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg
    18901 gattgaatcc ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt
    18961 cgatggtcat ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt
    19021 atcaaaactt ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt
    19081 ccttgcttgg ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt
    19141 tgaagatcgg tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg
    19201 tatcgattgc tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg
    19261 ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa
    19321 cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat
    19381 ccttacttct acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt
    19441 ttctgaacga agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt
    19501 tagttccatc gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat
    19561 cgctatttta gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac
    19621 agtcagtttg cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc
    19681 tttaggtgtt ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca
    19741 gaatctgggg tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt
    19801 ctttaaaaat acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt
    19861 gatcatcttc aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc
    19921 tgccgacaca ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg
    19981 cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc
    20041 tggtacgatc gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa
    20101 tcctttaggg gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat
    20161 tggcgcacaa ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata
    20221 tttgctgaca atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa
    20281 cgggaagaac tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg
    20341 aagtgttttt cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga
    20401 ataatcatgt ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca
    20461 ccagatgccg ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa
    20521 gcaggattga acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa
    20581 ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta
    20641 gggaaagata cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt
    20701 cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc
    20761 cgtaaagtcg tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa
    20821 caccaaatga aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc
    20881 gcggcacatg aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt
    20941 gatataacaa aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt
    21001 gaaccaggag acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga
    21061 cacacagtcc ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc
    21121 aacgatattt tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat
    21181 ggcgttgacc agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat
    21241 ttagtagatt ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg
    21301 attgaagaat ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg
    21361 ctaatgatta cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga
    21421 gaatatgtac cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa
    21481 ggatattatt cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa
    21541 aatggcgaaa gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga
    21601 ccaagttaag cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag
    21661 aaattgattt tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag
    21721 atgctgtaat gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc
    21781 atgcaggaaa gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga
    21841 gattccacta ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg
    21901 ccggactagg ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat
    21961 ttgtaccagg agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt
    22021 tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata
    22081 cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag
    22141 aaggcgtata tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt
    22201 ctcggacaat aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag
    22261 ctcacagcgg attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc
    22321 aagcgaatct gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta
    22381 aagcattggt taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg
    22441 catattgaag cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg
    22501 cgagctaaat atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc
    22561 ggtatgttag gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga
    22621 atgggaatgc catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag
    22681 aaattgattc gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta
    22741 gtactagcac aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt
    22801 cattttcctc caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa
    22861 aagggctata cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat
    22921 ttgacagaga cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg
    22981 gctctttatt acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc
    23041 gaccatttga tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa
    23101 atgatcgaag taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc
    23161 ctgcattaag taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg
    23221 gacttattat tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaatctgcc
    23281 gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat
    23341 tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa agggactgtg
    23401 acaaatcgtg tcacagtccc ttttttatag tgttttttat aggtagtatt ttttagcaac
    23461 tgttaagtca tcatttaatt cataaacaag tggttgacct gttggaattt caagatccat
    23521 gatgtcttcg tcagagatac cttcgatatg tttagctagg gcgcgtaatg agttaccatg
    23581 tgcagctact aaaacagttt tgttgtctag taatgctgga gcaatttcat cttgccagaa
    23641 tggcaaagcg cgttctaatg tcactttcaa gttttctcca ccaggaatat cgcgtttgtc
    23701 taacattgcg taacgacggt catttgctgc agaaccttcg tcagtttctt ccattaatgg
    23761 aggaagagta tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt atttttcagc
    23821 tgtttctttt ttgttcaaac cttgtaattt accatagtga cgttcgttta agcgccatga
    23881 tttgatttgt ggtacccaaa gttgatctga atattctaag atcaagttac atgttttgat
    23941 tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg attccagctt ctttgatttt
    24001 gcgtccgcct tcttttgctt cttcgattcc ttctggtgct agatttacat cagcccaacc
    24061 agtaaattgg ttcaatgcat tccattcact aagaccgtga cgagaaaaaa ctaattttgg
    24121 catgtttagc tctcctttta agataggttt atatttctga aattttcagt tctgttttat
    24181 tgtacaattt tttggaagag atttccatcg aaaaattaga taagattcta tgagaaaaca
    24241 agtagagaat ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta ggtattagat
    24301 gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg ttccaactga
    24361 aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat agagacgtcg
    24421 gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc cttttttacg
    24481 agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca tgtttgctag
    24541 gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc cttcatcaag
    24601 actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta attgccgcgt
    24661 agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt gcatgtcagc
    24721 gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat atgtctcatg
    24781 gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta aatcaacgtc
    24841 atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct agcagtgatt
    24901 ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga tagttttttc
    24961 gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt atttacgacg
    25021 gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag aaaccatgtt
    25081 taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag ctaagctttc
    25141 tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa
    25201 gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt caaaaatatg
    25261 aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact tcccaagttt
    25321 atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg tagcgactat
    25381 tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag cactgatttc
    25441 atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct ttaacggtat
    25501 tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg tgatacgtaa
    25561 accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg ttgaaccggt
    25621 cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc cagctaattg
    25681 ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt tcctcactat
    25741 tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa gctttttcaa
    25801 aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt
    25861 tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga
    25921 tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag
    25981 acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa
    26041 cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc
    26101 tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg
    26161 ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg
    26221 tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac
    26281 agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg ttaccatatc
    26341 gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac
    26401 ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta
    26461 tatagaagta attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag
    26521 gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc
    26581 gcttgatcaa ccgtgtaatg gttgacggaa aacgcggtat tgcagctaac attatctata
    26641 atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag
    26701 caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact
    26761 accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag
    26821 taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca
    26881 tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg
    26941 ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct
    27001 tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag
    27061 aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg
    27121 cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa
    27181 tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac
    27241 aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa
    27301 tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc
    27361 gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg
    27421 aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa
    27481 tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag
    27541 caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg
    27601 accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa
    27661 cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag
    27721 ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg
    27781 aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac
    27841 tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt
    27901 acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag
    27961 aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga
    28021 ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag
    28081 gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga
    28141 tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg
    28201 ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc
    28261 ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag
    28321 ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc
    28381 gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact
    28441 tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc
    28501 ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac
    28561 gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag
    28621 aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca
    28681 ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc
    28741 cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg
    28801 aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc
    28861 cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg
    28921 atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg
    28981 gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa
    29041 ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag
    29101 atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat
    29161 ttcttgaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag
    29221 tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata
    29281 cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag
    29341 aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg
    29401 gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag
    29461 ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct
    29521 caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag
    29581 gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct
    29641 tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc
    29701 gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg
    29761 aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc
    29821 ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt
    29881 catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag
    29941 aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac
    30001 gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg
    30061 aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc
    30121 gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg
    30181 cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata
    30241 caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat
    30301 tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg
    30361 aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat
    30421 taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta
    30481 cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa
    30541 gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct
    30601 tttttattta ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga
    30661 aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga
    30721 aaaagccttg ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg
    30781 aattgcacag acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc
    30841 ccggacgctt tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc
    30901 aaaataggcg aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg
    30961 tatgaacacc gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact
    31021 ggagctagcg tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc
    31081 cgtgcgactc ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt
    31141 cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta
    31201 ccatctggtg taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta
    31261 ctcatgacca aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct
    31321 ggtgaattaa ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa
    31381 acaatggaaa cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt
    31441 ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc
    31501 attcgtgaat tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta
    31561 gatgttttcc aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc
    31621 caaggggtta tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac
    31681 catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt
    31741 cttgctggcc gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc
    31801 gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta
    31861 attactatca aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga
    31921 atgccgaatg tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac
    31981 gaagaaatct tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa
    32041 cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc
    32101 ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca
    32161 ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa
    32221 cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa
    32281 aacaacttgg tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa
    32341 caagttcttg ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac
    32401 gactttgctg ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat
    32461 gtgagtgtgc tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact
    32521 caaattgagg aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca
    32581 ctgaaaaatc aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg
    32641 caaacaaaac tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg
    32701 tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa
    32761 aaaaacgtcg caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg
    32821 aagctgctga ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat
    32881 taaaaagtac aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga
    32941 aatcacaaca tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg
    33001 taacaacaac ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg
    33061 cgtgatcgac ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata
    33121 tgatccaaac cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta
    33181 catcttagca cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga
    33241 tatcaaagtc ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa
    33301 cattgaaatg aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca
    33361 agtacttggt aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat
    33421 gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat
    33481 caacatcggt aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag
    33541 cgtaatgaac cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg
    33601 taaagcacca gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa
    33661 agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg
    33721 tgtcgcacat tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt
    33781 cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat
    33841 caaaacttgg tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt
    33901 ttatgatgga cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt
    33961 aggtgaattt gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa
    34021 acgctaattt gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa
    34081 ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag
    34141 gtaaaagtgt cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa
    34201 tcattgaaaa agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg
    34261 ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc
    34321 gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag
    34381 tcgtatcaga aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg
    34441 cgtgtaggca tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag
    34501 ttcttacacg aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct
    34561 gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct
    34621 aaaccaggta tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta
    34681 aacaaattaa ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta
    34741 gatgcaaaat tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt
    34801 cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact
    34861 caagtatctg gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga
    34921 actgttcctc ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca
    34981 acatacggaa aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa
    35041 aaaaacactg agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc
    35101 gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg
    35161 aatacggttt gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc
    35221 gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc
    35281 acaaatctta tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg
    35341 aaggatgggt tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg
    35401 aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga
    35461 tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta
    35521 accactgccg aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga
    35581 ttccaattag caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg
    35641 attgcacgca tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt
    35701 aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac
    35761 aaaatggata aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc
    35821 aaacgcatga actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt
    35881 ggcgacatcg tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg
    35941 ttggaagtag tcgaagaagc agttattatc taataacacg agattttcct atatagattg
    36001 aatgcaaaat ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc
    36061 gctgataact caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt
    36121 aaaactgcta acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg
    36181 gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt
    36241 cgtacagacg gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat
    36301 aaaagccctc gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac
    36361 ttcatgaaga tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg
    36421 aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa
    36481 gacaaaaaca aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc
    36541 gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga
    36601 attgttgaga tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt
    36661 gtagctggcc gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa
    36721 accggtgaag ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc
    36781 ctaaaagaaa aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc
    36841 tctgttatgc aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg
    36901 gtatcaaacg ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa
    36961 aaaccaatga ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg
    37021 ccaattggtg caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg
    37081 gtttctgttt cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac
    37141 ggtcgtggaa actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat
    37201 gatttagtag ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat
    37261 gaagaatctc gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag
    37321 gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca
    37381 acacaagctt atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc
    37441 catctttgcc gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag
    37501 aaagctagct ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga
    37561 cagatccaat tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt
    37621 ctttagaagt acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag
    37681 gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc
    37741 taaaatttgg taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag
    37801 gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg
    37861 caatcatttc gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg
    37921 gcggcgaagt tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa
    37981 gtgagccgta tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac
    38041 ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat
    38101 atcaaaatga atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa
    38161 atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc
    38221 gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga
    38281 tcaaaacttg tattgaacgt tggttactca catccagtag aaatcacacc accagctgga
    38341 gtaactgttg aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa
    38401 gttggcgaat tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa
    38461 ggtattcgtt atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg
    38521 tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt
    38581 caaaaaaata catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt
    38641 gaaatagtag tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa
    38701 caaaacacgt caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg
    38761 cccacgcttg aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt
    38821 agcgggtgta acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa
    38881 aacagaagca gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat
    38941 taaagtagta gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc
    39001 tgaagctgct cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt
    39061 tatatcgatc caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca
    39121 aaagttgtta aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa
    39181 aacggacacg tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa
    39241 gcaattgaag atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca
    39301 cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt
    39361 tctggggttg ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat
    39421 atcacttcta aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa
    39481 ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa
    39541 gaattaatcg gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca
    39601 gtattatcgg acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga
    39661 ttaacagttc agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt
    39721 ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga
    39781 ggtgccaaac ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt
    39841 aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa
    39901 ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca
    39961 ttgttccgtc gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc
    40021 attaatcttg atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta
    40081 gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa
    40141 ctaaacaaga aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt
    40201 gaagcggctg gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta
    40261 aagtaaaaga cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc
    40321 taggtgcaca tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt
    40381 tgccattttt caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt
    40441 tctcaatggg ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg
    40501 acatcgtgcc tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga
    40561 atcaagcgac tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg
    40621 ctggattcaa tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg
    40681 taacgatcgg tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga
    40741 tcacagaaaa aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta
    40801 gattgcctga aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag
    40861 acatatggaa atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga
    40921 cattcgtaac atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg
    40981 tagctggcgc gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc
    41041 cagttatctt tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg
    41101 gtagattcgg ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag
    41161 cacctggtgc tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg
    41221 ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa
    41281 gcgtacgccc tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta
    41341 cagttggtgc agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt
    41401 gggatcttcc acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag
    41461 ctttagaaac tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca
    41521 ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga
    41581 gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct
    41641 gaaaaaatca ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca
    41701 gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca
    41761 ttagttcctg atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca
    41821 gagaaaggct tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac
    41881 gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa
    41941 gaaatcttgg tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac
    42001 cataaaattt tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa
    42061 ttctatcaaa gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc
    42121 aaaaatagtg aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat
    42181 ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac
    42241 gattgcccgt agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat
    42301 agaatatgtc agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag
    42361 gtgggaactg cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt
    42421 actgtgcgtg gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc
    42481 gaatgcaatg tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg
    42541 taaaattcgt atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc
    42601 tccatacgat cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa
    42661 actacaggag gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt
    42721 aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa
    42781 caacgtcaag gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc
    42841 ctcgtgataa acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg
    42901 ctaaaaagat cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga
    42961 atgaacaaac agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc
    43021 gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc
    43081 gtcatcgccg tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta
    43141 aaggcccagc tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa
    43201 acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag
    43261 gtattgcaca tatccattct acattcaata acactatcat catgatcact gatactcacg
    43321 gaaatgcatt agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa
    43381 ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat
    43441 taaagactgt tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt
    43501 cattacaagc tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata
    43561 atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta
    43621 tcagtaacgt cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt
    43681 gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc
    43741 gttgaacctc ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta
    43801 ttatcttctt tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa
    43861 ttctctacca tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc
    43921 ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct
    43981 gctacagtaa ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat
    44041 atgtacatct gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct
    44101 ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt
    44161 ccagtcgatt caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt
    44221 gttggacacc gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt
    44281 gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt
    44341 gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa
    44401 aaagaaaaaa tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat
    44461 tgtttgaaac gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa
    44521 atgatcaaag tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac
    44581 gacttaggtt taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac
    44641 gtgagttatc gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta
    44701 acaactgatt tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt
    44761 cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt
    44821 caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc
    44881 gtcattgaat cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt
    44941 caaggcggat acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg
    45001 gttgtcttag aacttgtcta aaatcacact aataacatca ctttatagat gattctttta
    45061 gagtgttatg atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga
    45121 atatcgcttg gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt
    45181 catgacagat atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt
    45241 tgtctttttt tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc
    45301 aattattgaa ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt
    45361 gaaagatgtc tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg
    45421 ttcaggaaaa tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat
    45481 cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt
    45541 tggaatggtt tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt
    45601 tgcttttggt cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga
    45661 tgcacttgag aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg
    45721 agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat
    45781 tttagatgaa gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat
    45841 caagaagatc aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga
    45901 agcagctaat gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac
    45961 accaaaagaa attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt
    46021 tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat
    46081 gacagaagaa aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact
    46141 tatcagccga atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa
    46201 gaaaacagtt atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag
    46261 catttgaatg cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt
    46321 cagccggata cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt
    46381 cagtttccag aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct
    46441 aaaaactttg gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa
    46501 gttgggctgg atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg
    46561 cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa
    46621 ccaacggcag gacttgatcc ccagggtaga aaagaaatga tggagatgtt ttggcgtctt
    46681 cataaggaac agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat
    46741 tttgcagatt atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa
    46801 gaggtctttc aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc
    46861 gagtttgcag aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct
    46921 gaagaattag cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt
    46981 gatatttggt cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa
    47041 attattagcc agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta
    47101 ccttttgttg gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt
    47161 ttttatccga ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt
    47221 cttgtttaca agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt
    47281 tggtgtgatg aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac
    47341 tctactcacg cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg
    47401 tccattgaaa gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct
    47461 tcgttttgtg cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg
    47521 cggtgttgat tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct
    47581 gattccttta tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc
    47641 aagaggctac aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag
    47701 agacacgatg gttatcattg cttttgtatt attaactgtg ctactgatct ttttgagagg
    47761 ataaacgttc agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat
    47821 gcgagcagtg tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt
    47881 tcttttatct gaaaagaaag aacgaaaaaa ttttataaaa aaatcattat tccagataac
    47941 cttttaggaa caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcatacgacg
    48001 gaacaaattt taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag
    48061 aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga
    48121 cagatgccgg agtacatgcg attgggcaag taatccattt tgattatcca gaggagcgtc
    48181 cgttagaacg aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac
    48241 aagtagaaat tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc
    48301 aatttcgagt ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt
    48361 ttccttaccc tcttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc
    48421 acgatttcac ctctttttgt gcatcaggaa gctcgataga ggataaagta cgtacgatct
    48481 acgaggctaa gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa
    48541 atggcttctt gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg
    48601 gccgattgcc tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag
    48661 gacctacagc acatcctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggttg
    48721 tgacagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattac ttctcaaaat
    48781 tttgcaaaat ttcggcttat tttcgaagga gctacttctg gaacaccgtg aatcagaggt
    48841 tgtgaaaaag ctgccctgca tcaagtaata agaacaacca aacaaaaata gctcgtcata
    48901 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    48961 aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggaaa aatagttcct
    49021 catattttgc cgtttgttga gctaataccg aaggtcagct gattgaacac cgtttaagaa
    49081 agaggtcgtg agcctgtcat ttaactctga gtcacaagta acaatttcaa aaaacagctt
    49141 ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt
    49201 gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg cccttaatga
    49261 ggacagtcgg aacaggtttg tcatttttat atttaaatgg cacaaatttt tctaattatt
    49321 tgtcatttag cgctgaaaat ttttgctatt tatctaaaaa agtgataact acttaagatg
    49381 aagcagaata tttacgccat acggcataca accgcgaaaa atataaaaaa aagaaaacga
    49441 ttgaacgcgt ttttgcagtc gcaaaggaaa agcatggaat gcgctacatc aagtatcgag
    49501 acttggaaaa caatcaggtg tatacgctga ttgtttttgc tgcaatgaat ctgaaaaagt
    49561 tgtccacttg gcagttggaa catatgtctt catatctttt attatacaaa aatagcaaaa
    49621 gcaccagtct acactcttag taaagaggaa actgatcctt ttgtctacat tcttaaagta
    49681 cttaaactaa gtactttatt ttactcttta ggattgatac agtagcatct atccctttat
    49741 taataatctt ttattttttt aaataaagat gttgtctcca tcttcaggca ctaaaacttt
    49801 ttcggaaatt cctttttctt tattgaaaga ctttaattct tctcttgaca gtccccaatg
    49861 gtttacagct tccatatgaa cggcaataat tgtggaatct ggtattgctt tatgcaattc
    49921 atataaatcg tctttcccca taataagaga ttctccaact aaaaaatgat tatctccagc
    49981 atttgctact atgatttctg gattatactg atcaatagtt tgtttaacag cttcatacca
    50041 aactgtatct cctgtaaagt atagtgtctt ctcagtcgaa tgtttgaaaa caataccgca
    50101 aacattacct acataattca agatttctcc tctgccatgt ttgccttttg ttttaaccaa
    50161 ttgtatagat tcaaaaactg tgtcttctcg tagtacctcc acatttttaa aaccatatag
    50221 tcgaatttca ttcgcatcat tttcatcttg cacaaataat tttatttctt taggtaggac
    50281 ctctttcgct atatcgtccc aatgatctag atgtaaatgt gttacaataa cagcatcaat
    50341 accgttgata atatcttcga ctggcattgg caaatcaact agaggattca tttgatcttt
    50401 tcttggtgat ggaaaaggac ctttcgaaat aaatggtgga agtgttcctt tttccgctaa
    50461 cattggatca attaaaaatt ttttacctcc atagtctaag atagctgttg cattacgaat
    50521 gtgatgtata ttcataaacg ttgtctcctt tttgttgata tgaaatattt ttagtataat
    50581 tcaattccat acaataaata aaccgaacaa aaaaagtctt ttactatttt tacttaattt
    50641 atgaaaggtt gtttaccatg ttagaccaaa cagatataaa tatcattgag gaactatcaa
    50701 aaaatagtcg aatcacaatg aaagaacttg gagaaaaagt acatttgact ggacccgctg
    50761 tctcggcgag agtgacaaaa ctagaggaaa gtggagttat tgaaaattat acagtaaaat
    50821 taaatatgga aaagctaggg ttttcgattc acgctttttt aattataatt acgcaaaatt
    50881 taaatcataa accctattta tcatttatta aaaatcatga aa
//
LOCUS       DASJSZ010000010        50067 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.010, whole genome shotgun sequence.
ACCESSION   DASJSZ010000010 DASJSZ010000000
VERSION     DASJSZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.010"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..629)
                     /locus_tag="U4B03_000553"
     CDS             complement(105..629)
                     /locus_tag="U4B03_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2067429.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U4B03_000554"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U4B03_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEN2067430.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U4B03_000555"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U4B03_000555"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEN2067431.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="U4B03_000556"
     CDS             complement(2351..3031)
                     /locus_tag="U4B03_000556"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN2067432.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="U4B03_000557"
     CDS             3157..4026
                     /locus_tag="U4B03_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2067433.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="U4B03_000558"
     CDS             4052..4567
                     /locus_tag="U4B03_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067434.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="U4B03_000559"
     CDS             complement(4614..5207)
                     /locus_tag="U4B03_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2067435.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="U4B03_000560"
     CDS             complement(5232..5411)
                     /locus_tag="U4B03_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067436.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="U4B03_000561"
     CDS             complement(5488..5997)
                     /locus_tag="U4B03_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067437.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="U4B03_000562"
     CDS             complement(6093..6473)
                     /locus_tag="U4B03_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2067438.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="U4B03_000563"
     CDS             6803..7552
                     /locus_tag="U4B03_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEN2067439.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="U4B03_000564"
     CDS             complement(7736..9013)
                     /locus_tag="U4B03_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067440.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="U4B03_000565"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="U4B03_000565"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEN2067441.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="U4B03_000566"
     CDS             complement(9935..10879)
                     /locus_tag="U4B03_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEN2067442.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="U4B03_000567"
     CDS             complement(10881..11567)
                     /locus_tag="U4B03_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEN2067443.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="U4B03_000568"
     CDS             12041..12361
                     /locus_tag="U4B03_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEN2067444.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="U4B03_000569"
     CDS             12515..13420
                     /locus_tag="U4B03_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2067445.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="U4B03_000570"
     CDS             13417..14190
                     /locus_tag="U4B03_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN2067446.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="U4B03_000571"
     CDS             complement(14371..15795)
                     /locus_tag="U4B03_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2067447.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="U4B03_000572"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="U4B03_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN2067448.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="U4B03_000573"
     CDS             complement(16517..18499)
                     /locus_tag="U4B03_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2067449.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="U4B03_000574"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="U4B03_000574"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN2067450.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="U4B03_000575"
     CDS             complement(19412..20473)
                     /locus_tag="U4B03_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN2067451.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21250)
                     /locus_tag="U4B03_000576"
     CDS             complement(20684..21250)
                     /locus_tag="U4B03_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2067452.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="U4B03_000577"
     CDS             complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="U4B03_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN2067453.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="U4B03_000578"
     CDS             complement(23038..24159)
                     /locus_tag="U4B03_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2067454.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="U4B03_000579"
     CDS             24285..25226
                     /locus_tag="U4B03_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN2067455.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="U4B03_000580"
     CDS             complement(25376..26272)
                     /locus_tag="U4B03_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN2067456.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="U4B03_000581"
     CDS             26541..27881
                     /locus_tag="U4B03_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2067457.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="U4B03_000582"
     CDS             28099..28542
                     /locus_tag="U4B03_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN2067458.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="U4B03_000583"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="U4B03_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN2067459.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="U4B03_000584"
     CDS             complement(30867..32090)
                     /locus_tag="U4B03_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEN2067460.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="U4B03_000585"
     CDS             complement(32121..32960)
                     /locus_tag="U4B03_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN2067461.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="U4B03_000586"
     CDS             complement(32977..33657)
                     /locus_tag="U4B03_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2067462.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="U4B03_000587"
     CDS             complement(33659..34732)
                     /locus_tag="U4B03_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2067463.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(34788..35026)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(35069..35302)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35379..36050)
                     /locus_tag="U4B03_000588"
     CDS             complement(35379..36050)
                     /locus_tag="U4B03_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2067464.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="U4B03_000589"
     CDS             complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="U4B03_000589"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEN2067465.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="U4B03_000590"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="U4B03_000590"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEN2067466.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40799)
                     /locus_tag="U4B03_000591"
     CDS             complement(39933..40799)
                     /locus_tag="U4B03_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2067467.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40959..43052)
                     /locus_tag="U4B03_000592"
     CDS             complement(40959..43052)
                     /locus_tag="U4B03_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2067468.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(43801..44424)
                     /locus_tag="U4B03_000593"
     CDS             complement(43801..44424)
                     /locus_tag="U4B03_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067469.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44583..45980)
                     /locus_tag="U4B03_000594"
     CDS             complement(44583..45980)
                     /locus_tag="U4B03_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2067470.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(46012..46983)
                     /locus_tag="U4B03_000595"
     CDS             complement(46012..46983)
                     /locus_tag="U4B03_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2067471.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            47357..47782
                     /locus_tag="U4B03_000596"
     CDS             47357..47782
                     /locus_tag="U4B03_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067472.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(48270..48971)
                     /locus_tag="U4B03_000597"
     CDS             complement(48270..48971)
                     /locus_tag="U4B03_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2067473.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(48987..49427)
                     /locus_tag="U4B03_000598"
     CDS             complement(48987..49427)
                     /locus_tag="U4B03_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN2067474.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(49427..50002)
                     /locus_tag="U4B03_000599"
     CDS             complement(49427..50002)
                     /locus_tag="U4B03_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEN2067475.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT        14430 a        10481 c         8733 g        16423 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaataacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtga taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    40801 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    40861 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    40921 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    40981 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    41041 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    41101 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    41161 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    41221 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    41281 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    41341 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    41401 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    41461 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    41521 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    41581 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    41641 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    41701 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    41761 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    41821 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    41881 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    41941 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    42001 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    42061 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    42121 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    42181 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    42241 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    42301 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    42361 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    42421 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    42481 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    42541 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    42601 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    42661 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    42721 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    42781 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    42841 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    42901 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    42961 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    43021 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    43081 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    43141 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    43201 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    43261 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    43321 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    43381 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    43441 gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    43501 cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg
    43561 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
    43621 ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac
    43681 acggtgttca atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact
    43741 attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt
    43801 tcattgtcca taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg
    43861 attttcttca cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt
    43921 cgactcgttt ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca
    43981 ttccgctaaa cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac
    44041 ttttccattt ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa
    44101 atgcttgtct ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt
    44161 aataaattga ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta
    44221 attggcagtt attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc
    44281 tactgattct ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat
    44341 ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag
    44401 aagaaacggt ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta
    44461 ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa
    44521 cttctttttc acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt
    44581 tcttaagcct caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg
    44641 attgttcttt ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat
    44701 gcattggtcc acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca
    44761 ccttcttgaa tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa
    44821 tcatcgtgga tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg
    44881 ttttcagaaa taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca
    44941 tagattcctt gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt
    45001 tcataattgt cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct
    45061 tttgctttta cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct
    45121 ttgataaccg tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc
    45181 tcaatcaatt cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag
    45241 aacgcatctg ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag
    45301 cttggtgtca gattcaagat gataccaata acaccacctt gctccatttc atggtaagat
    45361 ttgatggcta acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata
    45421 tctgactctt ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt
    45481 tcattatgag taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca
    45541 tatttttcat aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac
    45601 atcggcatat cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca
    45661 tcaaaataac gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt
    45721 atcaaacggc tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac
    45781 tgtacatcct cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca
    45841 aagaactttt gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg
    45901 cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct
    45961 ggaaattgat attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat
    46021 tccatgtatg atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat
    46081 tcgttttaac agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt
    46141 gattttcgga aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat
    46201 atccttcccg attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt
    46261 gtaattgctg acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata
    46321 gccgcttttt ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg
    46381 gaccgcttct ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc
    46441 aataaatccg atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact
    46501 ttctcgatca ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc
    46561 gataactgga atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa
    46621 gaagacaatc agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt
    46681 ttctggctga tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct
    46741 ttcgatccct gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc
    46801 aatagagaag cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa
    46861 cgctgaaatc gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa
    46921 gtaacgggaa actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc
    46981 catacattct cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat
    47041 gaccgcattc tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt
    47101 agagcaactt cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt
    47161 aatatagcat ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg
    47221 attttttgcc tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg
    47281 tttttctctt ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc
    47341 aaatggggga agtattttga agaaattaat cgtactattt attttgtctg cgggactttt
    47401 gacattgact ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga
    47461 ttcttctgtc ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt
    47521 acgtaaagat atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg
    47581 gacacgcacg tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt
    47641 gctgacagat atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa
    47701 taaaaaagaa acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga
    47761 tggcaacatc tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt
    47821 ttctgctata gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc
    47881 ctgattattg tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa
    47941 gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat
    48001 cttatcctgt tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc
    48061 actatggctg atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag
    48121 taaaacaaac agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca
    48181 aatatttgaa tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt
    48241 tttctttccc gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg
    48301 ttatccgtaa aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc
    48361 tgacggatca ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc
    48421 acttgtattt taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac
    48481 gtttcataat aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt
    48541 tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc
    48601 cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac
    48661 tgctgtactt cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta
    48721 tcttcataga tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct
    48781 aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa
    48841 taaaagagcg gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc
    48901 tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa
    48961 atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc
    49021 aatgtttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt
    49081 tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc
    49141 aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag
    49201 tcaaggatag caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact
    49261 tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag
    49321 tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg
    49381 atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct
    49441 tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat
    49501 tgttggaaaa gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag
    49561 tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct
    49621 tgtcgggcgt cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac
    49681 ctagacctcc agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt
    49741 catcgatcgt ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa
    49801 aaagcccatt cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa
    49861 tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca
    49921 tgtattttcc taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac
    49981 cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc
    50041 tacacgtgtt tttttaaaat aaaagac
//
LOCUS       DASJSZ010000011        48287 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.011, whole genome shotgun sequence.
ACCESSION   DASJSZ010000011 DASJSZ010000000
VERSION     DASJSZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.011"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(49..1557)
                     /locus_tag="U4B03_000600"
     CDS             complement(49..1557)
                     /locus_tag="U4B03_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEN2067476.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1716..1907
                     /locus_tag="U4B03_000601"
     CDS             1716..1907
                     /locus_tag="U4B03_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067477.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2110..3027
                     /locus_tag="U4B03_000602"
     CDS             2110..3027
                     /locus_tag="U4B03_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEN2067478.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3020..4093
                     /locus_tag="U4B03_000603"
     CDS             3020..4093
                     /locus_tag="U4B03_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067479.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4241..4759
                     /locus_tag="U4B03_000604"
     CDS             4241..4759
                     /locus_tag="U4B03_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067480.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4740..5132
                     /locus_tag="U4B03_000605"
     CDS             4740..5132
                     /locus_tag="U4B03_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEN2067481.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5208..6143
                     /locus_tag="U4B03_000606"
     CDS             5208..6143
                     /locus_tag="U4B03_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEN2067482.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6276..7055
                     /locus_tag="U4B03_000607"
     CDS             6276..7055
                     /locus_tag="U4B03_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN2067483.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7048..7365
                     /locus_tag="U4B03_000608"
     CDS             7048..7365
                     /locus_tag="U4B03_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN2067484.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7609..9219
                     /locus_tag="U4B03_000609"
     CDS             7609..9219
                     /locus_tag="U4B03_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2067485.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9376..10224
                     /locus_tag="U4B03_000610"
     CDS             9376..10224
                     /locus_tag="U4B03_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2067486.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10455..10934
                     /locus_tag="U4B03_000611"
     CDS             10455..10934
                     /locus_tag="U4B03_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067487.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            10967..13195
                     /locus_tag="U4B03_000612"
     CDS             10967..13195
                     /locus_tag="U4B03_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2067488.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13324..14967
                     /locus_tag="U4B03_000613"
     CDS             13324..14967
                     /locus_tag="U4B03_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN2067489.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15040..15585)
                     /locus_tag="U4B03_000614"
     CDS             complement(15040..15585)
                     /locus_tag="U4B03_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEN2067490.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15608..16093)
                     /locus_tag="U4B03_000615"
     CDS             complement(15608..16093)
                     /locus_tag="U4B03_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2067491.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16083..16895)
                     /locus_tag="U4B03_000616"
     CDS             complement(16083..16895)
                     /locus_tag="U4B03_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEN2067492.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(17034..18146)
                     /locus_tag="U4B03_000617"
                     /pseudo
     CDS             complement(17034..18146)
                     /locus_tag="U4B03_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            complement(18167..19075)
                     /locus_tag="U4B03_000618"
     CDS             complement(18167..19075)
                     /locus_tag="U4B03_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HEN2067493.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFES
                     ILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            complement(19169..20218)
                     /locus_tag="U4B03_000619"
     CDS             complement(19169..20218)
                     /locus_tag="U4B03_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2067494.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            complement(20300..20989)
                     /locus_tag="U4B03_000620"
     CDS             complement(20300..20989)
                     /locus_tag="U4B03_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2067495.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(21095..21463)
                     /gene="rplL"
                     /locus_tag="U4B03_000621"
     CDS             complement(21095..21463)
                     /gene="rplL"
                     /locus_tag="U4B03_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEN2067496.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(21516..22019)
                     /locus_tag="U4B03_000622"
     CDS             complement(21516..22019)
                     /locus_tag="U4B03_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEN2067497.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(22223..22350)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(22429..23118)
                     /gene="rplA"
                     /locus_tag="U4B03_000623"
     CDS             complement(22429..23118)
                     /gene="rplA"
                     /locus_tag="U4B03_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEN2067498.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(23222..23644)
                     /gene="rplK"
                     /locus_tag="U4B03_000624"
     CDS             complement(23222..23644)
                     /gene="rplK"
                     /locus_tag="U4B03_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEN2067499.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            23987..24655
                     /gene="sdaAB"
                     /locus_tag="U4B03_000625"
     CDS             23987..24655
                     /gene="sdaAB"
                     /locus_tag="U4B03_000625"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEN2067500.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            24668..25540
                     /gene="sdaAA"
                     /locus_tag="U4B03_000626"
     CDS             24668..25540
                     /gene="sdaAA"
                     /locus_tag="U4B03_000626"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEN2067501.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(25631..26650)
                     /locus_tag="U4B03_000627"
     CDS             complement(25631..26650)
                     /locus_tag="U4B03_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN2067502.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(26823..27728)
                     /locus_tag="U4B03_000628"
     CDS             complement(26823..27728)
                     /locus_tag="U4B03_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2067503.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(27787..28332)
                     /gene="nusG"
                     /locus_tag="U4B03_000629"
     CDS             complement(27787..28332)
                     /gene="nusG"
                     /locus_tag="U4B03_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEN2067504.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(28433..28603)
                     /gene="secE"
                     /locus_tag="U4B03_000630"
     CDS             complement(28433..28603)
                     /gene="secE"
                     /locus_tag="U4B03_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEN2067505.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(28627..28779)
                     /gene="rpmG"
                     /locus_tag="U4B03_000631"
     CDS             complement(28627..28779)
                     /gene="rpmG"
                     /locus_tag="U4B03_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2067506.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            28941..29585
                     /locus_tag="U4B03_000632"
     CDS             28941..29585
                     /locus_tag="U4B03_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2067507.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(29641..30144)
                     /locus_tag="U4B03_000633"
     CDS             complement(29641..30144)
                     /locus_tag="U4B03_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN2067508.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(30157..30203)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            30741..31706
                     /locus_tag="U4B03_000634"
     CDS             30741..31706
                     /locus_tag="U4B03_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN2067509.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(31805..32977)
                     /locus_tag="U4B03_000635"
     CDS             complement(31805..32977)
                     /locus_tag="U4B03_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2067510.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(32964..34109)
                     /locus_tag="U4B03_000636"
     CDS             complement(32964..34109)
                     /locus_tag="U4B03_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2067511.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(34123..35055)
                     /locus_tag="U4B03_000637"
     CDS             complement(34123..35055)
                     /locus_tag="U4B03_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2067512.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            35172..36251
                     /locus_tag="U4B03_000638"
     CDS             35172..36251
                     /locus_tag="U4B03_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2067513.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            36248..36866
                     /locus_tag="U4B03_000639"
                     /pseudo
     CDS             36248..36866
                     /locus_tag="U4B03_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(36976..37203)
                     /locus_tag="U4B03_000640"
     CDS             complement(36976..37203)
                     /locus_tag="U4B03_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2067514.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(37306..39894)
                     /locus_tag="U4B03_000641"
     CDS             complement(37306..39894)
                     /locus_tag="U4B03_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEN2067515.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(39914..40573)
                     /locus_tag="U4B03_000642"
     CDS             complement(39914..40573)
                     /locus_tag="U4B03_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2067516.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(40622..41125)
                     /gene="trmL"
                     /locus_tag="U4B03_000643"
     CDS             complement(40622..41125)
                     /gene="trmL"
                     /locus_tag="U4B03_000643"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEN2067517.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(41346..42212)
                     /locus_tag="U4B03_000644"
     CDS             complement(41346..42212)
                     /locus_tag="U4B03_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2067518.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            42321..42962
                     /locus_tag="U4B03_000645"
     CDS             42321..42962
                     /locus_tag="U4B03_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEN2067519.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(43024..44385)
                     /gene="mgtE"
                     /locus_tag="U4B03_000646"
     CDS             complement(43024..44385)
                     /gene="mgtE"
                     /locus_tag="U4B03_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEN2067520.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(44403..45302)
                     /locus_tag="U4B03_000647"
     CDS             complement(44403..45302)
                     /locus_tag="U4B03_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2067521.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(45304..46101)
                     /locus_tag="U4B03_000648"
     CDS             complement(45304..46101)
                     /locus_tag="U4B03_000648"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEN2067522.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(46079..46783)
                     /locus_tag="U4B03_000649"
     CDS             complement(46079..46783)
                     /locus_tag="U4B03_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEN2067523.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            46914..47489
                     /locus_tag="U4B03_000650"
     CDS             46914..47489
                     /locus_tag="U4B03_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEN2067524.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            47607..48284
                     /locus_tag="U4B03_000651"
     CDS             47607..48284
                     /locus_tag="U4B03_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN2067525.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT        14763 a         9651 c         9046 g        14827 t
ORIGIN      
        1 tcgattaagc taatagtttc gttttcaaca tttataagta aactttttct attggtaata
       61 tagattttcg gaagggtaga caggtgcgca ggtaatatcg attcctagtt ttttcaatgt
      121 ttgttcatca tttttattga ccatcacagt agagtgggct tgtgctccct caagttcagc
      181 tagtttatca taagcaactt gagcgattgg attcgttact gcactgattg ataaagcgat
      241 caagacttca ttgatgctta gtgcatccaa agaggaatga agtgtctttg atttcaatcc
      301 ttgtattgtt tcaagaacta atggtgacaa taaaggaatg tcatctgcga tatgggctaa
      361 atgttttact gcatttaaga taactgccgc tgacgcatcc atcaatgaac ttgtacgccc
      421 ggtaatgatg acttcatctg gtaattcgat tgcaatcaca gcagaaggat cttttgtagg
      481 actttgcgct tgaatcttat ctgcatagtc atgggcaggg actaccacgt ttcggtcttc
      541 aggacgtaaa ccgacttctt ccataatcag tttgattcga ttaactgctt cttcgtcgat
      601 caatcctttt ttgaagtcgc attctgtttg gaaataacga cggatgattt cttgtttaga
      661 ggcttcttgg acaacggtat catccacgat cccaaatcct acacgattca cacccatgtc
      721 agtaggagat tgataaacag attctttacc agtaattttt tcaataatac gtttgattac
      781 aggaaatgtc tcaacatccc gattatagtt cacagctact ttattgtagg catcaaagtg
      841 gaaggaatcg atcatattga catcttttag atcgactgtg gctgcttcat aagcaatgtt
      901 cagcggatgt ttcaacggaa cattccatac tgggaaagtt tcgaacttgg agtaaccagc
      961 tgcatgtcct ttttggcttt catgatacag ttggttcaag gaagtagcga gctttccact
     1021 tcctgggcca ggagctgtca ctacaacgat tggtttagtt gtttcaatat actcattttt
     1081 tccaaaacca tcttctgaga cgattttttc tacgttgact ggatagtctt cgatttctga
     1141 gtgtttatag accttgatgt ttcgtctttc tagtttattg ataaagactt tcgtggcagg
     1201 ctgtccacta tagcgagtga taacgacact gttgattaat aagccataac cacgtaattc
     1261 atcgattaac cgcaagatat ccatatcata agtaatccca taatcaccac ggatcttatt
     1321 acgttcaata tctccagcat agacgcaaat cagtatttca gcttgatctt tcaatttttg
     1381 cagcaatttg attttcgcat cttcttcaaa tcctggaagt acacgtttcg catgcttgtc
     1441 gtcaactagc ttcccaccaa attctaaata taatttgtca tagtggttca ctcgttccaa
     1501 aatatacgct gactgctctt caatatattt ttctgaatca aaaccaattt ttttcactag
     1561 gttccctcct taagtactaa agcatttttt catttttttg aggattcgtc aactggaaga
     1621 gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag atagttattc
     1681 attggtatga tagtttttga taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa
     1741 acgcatataa acactatatc cgttcgattt ttaccggtta tgaaagaatg cttgaagatc
     1801 ccgaattgga acaattgacg tttacttatc taaacgaaga aacgcagcta actcgtgatg
     1861 agcatcagcg cattcatttc acaacaagag atctgcctag taaataaaaa ataagataaa
     1921 ggtcatcgga ttcgattctg tcctatcaga tggaaagact agttgatcga aaaaaaggct
     1981 gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc cgctcttttt tataacaatt
     2041 ttttacagaa aatggtacta ttgaagtatt aataagtgaa aaacagatga gtaagaggag
     2101 gcaagtagta tgaaagagcc agatacaaat gcaaattatg agccagacga actagaggag
     2161 acgaatgcgg atcaggatac aggacaagaa tcgtcttctt tttacgatat caaagaaaaa
     2221 gcaagcatga atctttccca attattagaa gacttacagg cttttgaaaa agctgtccgc
     2281 gaagaagata tccctgagat ctaccgttta tacaatggcc gattgaatga agaattaaaa
     2341 agaagctcaa atgaaaatca cgagatcgat caattgctga tgcgtaagat ccatgatcgt
     2401 tttttacagg aatttccttt tatggaacaa gtagaaaaca tctcgccaac gatgagctat
     2461 tacaaaatcg gcacttatta ccatgatcgg cccacggtcg gtattgatgc aagtctgcca
     2521 gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga aatattccca accggagtca
     2581 acggactatg acaaaaaaat caatgagcta gacgcaaagg tgatcactgc tcagacagaa
     2641 attgaacgtt tagatgaaca aataaaagaa atcaatcgac aaatgactga tttgaacgac
     2701 aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag
     2761 aagcaagtac tatctaatga aaaattaggc tggcttcctt atattgagac gcctgaaaca
     2821 atccagcagc aaaaagaagc attgaagcaa gaagcaagag cagaccagct tcgtgcagcc
     2881 atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga gggattcggt
     2941 caagcaatcc atgaattctt gatgaattat cttggcaatg agaatcctaa taatcaatct
     3001 aatgaaggag gttccgagta tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga
     3061 acaaacagaa gatcatgaag aaacgaacac ttcattggaa gaacaaacaa gtaatcctct
     3121 ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa atacacgaaa aagaaaaagt
     3181 aactccgacg aatacgaata ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc
     3241 cgggtattta gaaggaaaag agcaagatca tcaagatatc gtcagtttga ttgaaacagc
     3301 ggaagcagaa ttagcggatt tacgtatgag aaaagatttg atcgaatcag attttccaca
     3361 attattagaa tcatatgatc gttttatatt gaatgaagaa ccaatctcca cgatcggaaa
     3421 gacaagcctg atcgaatact tcacgtatga attactgaaa ccgttgaatg atattggtct
     3481 ggaacggtca aattcagctt atgacacatg gaaaacaaga catttttcga tcaattatac
     3541 gttgaatgga caaaacgaat tagtcttttc ctttgtatta ccgaccatcg atcaacgata
     3601 ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag acaatggaaa tcaatgtcca
     3661 agatgaccgg gtgctttctt tgatccgaca ctggtcagtg gatcgagtat tctctgcagg
     3721 gcagatcacg atattcaatc acaaattgaa tcaattatta gcccatgcaa gaacacttgg
     3781 ttttaccgtt aatcagacat tactagacaa tacgaagcca cttcgtttga atcttcagag
     3841 cgaatttgaa ttgacgaatg aagtattgga tgatattttt atcgtggcaa tgaaaaatcc
     3901 atcctacgat atggaaaaga tcaacgaaac tacttatcaa gtacgattag ataaaggaca
     3961 gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct catctggaga
     4021 ctacccacga tctgtcattg atttctttat caattacgaa ttcttggtcc cattaatggt
     4081 gagaaagtcc tgattggata agttggatta ccagatgtca ttcagataat tctgttccaa
     4141 aggacttgcg gcagttatcc agaataattg tcacaagtct tttctgtatg tactaaatgg
     4201 acgtgaataa cttgctgatg aaggagggat ttcattagga atgtccacaa ataaaatagc
     4261 acagcttagc ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc aatttattcc
     4321 gaatttccag ccaatgactg ctattttttt atttatagta ctttacttaa gcttaaaaga
     4381 tgcgttaatt gtcatgagtc tttctattgc gatcagcagt ttttattttg gtgttggacc
     4441 atgggtcatc ggccaatgga tcagctatac tgttgttctg agtttgtttt caatcgtctg
     4501 cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga gtctgttttt ttctggcggg
     4561 catggtttac ggaataggta tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc
     4621 ttggatctat taccttcaag gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt
     4681 gttctttctt gtttttctgc cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa
     4741 tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt
     4801 gcagtacaac ctatacacaa tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa
     4861 aagcaacaat cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa gtgttcttca
     4921 aaaaaggcga tgatttactg agtattttga aacgaaattt tgaagtcaaa gaagataacg
     4981 gcttcatcac ttcgcttgaa gggcactcac aggatgagaa aaatcaactc tattggatgt
     5041 ttactgtgga cgggaaaagt gctactactg gtgccaaaga gatcaaattg aatgatggac
     5101 aatcggtcat tttcaatttg tctaaactat aagtttcata aaaaactgtt aaagggcgaa
     5161 tattgccata attcagtgag ttcagttgat gaaagaagga aaataacatg gaaaatttta
     5221 aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc taatattcta gttgctatca
     5281 gcaaatttgt cggctatgca atcagtggca gtgccgccat gctaaacgaa agcatccaca
     5341 gtatcgttga ttgcagcaat cagattttgc tgttattcgg tgataaacaa gccaataaag
     5401 gacagagcga gttgcatcaa tttggtgaag gacgagcaaa atactttttc agtacaatcg
     5461 tcgcaatgat gcttttcttc ggcggtgggg cacttggggt gatggaagct tttgaaaagt
     5521 tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt tatagcaata ctgttatttg
     5581 gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata
     5641 ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa atactgatta
     5701 tttttacgga agacttgtgt gctgtaatcg gtttggtctt ggctttagca ggaacaatat
     5761 tgacttatgc aaccgggatt gctgcatttg atgcgatcag cggattgctg atcggtttct
     5821 tgctgatggg agccgccatt ttcctagcaa aagaatttta tagtctgatc atcggtgaaa
     5881 gcgtgacagc aagcgattta tcaaaaatca agttagcttt tgaacgtcct gagatcagtc
     5941 ggctgatcga tataaaaaca gttcatttaa gtccaacgga gattttagtt gcagcaaaag
     6001 cagatatcgt tggtaacaaa gagaatgaag cttactcggt catcaatgat attgaaaaaa
     6061 atatgcgtca gtctcttcct gacaagaaga tgtatatata cattgaaaca gataaatatg
     6121 acccaaatta ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa
     6181 tataatcttg tttttaaata aaacggctac aatttagcga atttatgtta caatgagtcc
     6241 gctgtttatt tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa agtgcgccat
     6301 ggcttcaagc tttgcatgca gtgatgccac tttgtataag ctatatacca gtaggattag
     6361 cttgcggagt attgttgcaa aaagttggat ttgatcctct cttatcagga ctgttatcga
     6421 ttctggtctt ttccggtggc gcgcagtttt tagttgcatc tatgcttacg acgcaagcat
     6481 catttgcaac gacattattg atggttttct ttttagaatt acgctataca ttattaggag
     6541 caagtcttgc cggttttatg aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat
     6601 cactgaatga tgaaaactat gcggtgaact acttaaagtt ttcaactgac ccttcctgga
     6661 ataaaagaaa ggctttatat gtcaattggt tctcaatgac tgcatgggcc ggtagcaata
     6721 tgatcggtaa ttttttcggc tcggtgattc gagtagatgc tgaccttgtc cattttgctt
     6781 tgacggccat gttcattttc atgtttatca tgcagatgaa aagcgcacta ctgatcttta
     6841 caggtttatt ctcaggcgta ttaggggtcg tattcatggt attattccaa aatactttcg
     6901 gattgattgc agcaacagtc attgcttcat ttgccggatt tatgctggag aaggcattca
     6961 aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca
     7021 acttcccgga acaggaggcc aatcaccatg actaaactat atttgctact tttagttgct
     7081 tgtctgttca tcgtggcata tatcccgcgc ttgttcccga tgctttattt tactcatcga
     7141 aaagtgccat cctggttcag tgactggatg aaatatgtac cggtggcatt atttgcggcg
     7201 ttagctttta aagatgtttt cattactcat gaacatctgg atattgcttg gaatatcaaa
     7261 atagctgcaa tgattctagt agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca
     7321 gttttgacag gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt tttattttat
     7381 taaaaacaga gattgcgaca taagttgttc aaactgatct atccgaactg catttgcaac
     7441 agtcaattct ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt ggcttatgat
     7501 gcaagctctt ttttatttta ggaattagag agggatttgt ccttctatga ttttttgcta
     7561 taatagggaa gatgtttatt ttatgaaaga atagttgagg gaatttaaat gaataaaaaa
     7621 ttgatgcaag gaacattttg gctaaccttt gctaatcttc tatgtaaagt tttaggggta
     7681 gtctatttga ttccctggct aagtatgatg gggaacaatc aagatggtat gcttgctacg
     7741 acattgtata atgttggcta tcttccttat ggattgttcc tgatgcttgg tacagtcgga
     7801 tttccaaatg cgattgcaaa aaaagtagct gtagccacaa aagaaggaga taacgctgct
     7861 tgtcggctga tttttagaag tacgatcaat atcatgtttg tcatcggtat cgcctctgct
     7921 gcactgatgt atttatttgc tcctttacta gccggaatca gtccgattgc taacgttaat
     7981 aatgggatac tagctatcag aagtttatgc ccatctttga ttgctatccc aattcttagt
     8041 gcaatgagag gatatttcca aggaaaaaat gatctgcggc cttatggaac ttctttgatt
     8101 atcgaacagg ctgtgcgagt gatcgttatc ttggctggta cttattactt gcgcgtattg
     8161 actgacggaa ccatattaga agctgtattg atcagtacag tcgcttcttt cttcggtggg
     8221 ttagctgcga ttatccatat gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa
     8281 gattttttga tttccagccg ttatttcgaa agagaaaacc gatctgcctc tgtctctatc
     8341 attcgggaaa cactaccgtt tatttttgtc ggctcagtca ttactatcgt tcaaatgatc
     8401 gaccaagtaa cgatgaaacc actgcttcac tttttccgtc cagagatcgc agatcagcaa
     8461 ctggaattct tattcagccg tgcttctgtc aatccaaata aattgacatt gatcctgatc
     8521 tccatggtag gaacagtagc tataagcagc ttgccaatat taagcacgat gaagaaaaaa
     8581 gatcggatac aaatcgaaaa aacagtagga gatagttttt cgattgcgtt attgattctt
     8641 cttccttcat tgactggaat gtctttatta gctggaccac tatacacatt gttttttggc
     8701 tatgatcctg aaagtgtcgg atatttccaa atggctttgc tagcttcttt gtttttctct
     8761 ttattcacga tcctatctac gatgcttcaa tctttgaatc accatcgttt tgcgatccga
     8821 ttaactgtag aagcaattat cctgaaagtc atcttccaag taatcggatt aggattagtt
     8881 ggcggatacg ggatgagctt atcaaatggc ttagcattcg gtattgtctt tgttcgcggc
     8941 tatcacttta tgtgcaaaga ataccgaata gcacctttag caaagatcag caattttttc
     9001 ttaaaaactt tccgctccac cctgattatg cttgcagtat gttttgccgt tttctttttg
     9061 ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa tgatttattg tgtggctgta
     9121 ggaagtattg gcggcttagt atttttattt tctcaattcg gaaaaaatgg gtttcgaatg
     9181 ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg ttaactaatc gttttttgct
     9241 gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg tttttctatt ttccaatttt
     9301 agtttcatat ctaatcttac ttatgatata gttataaggt ctggaaaaat tctttgaaac
     9361 gaggaaatta aacacatgaa acttgcagta gttacagata gttcggcata tctgccggaa
     9421 cgtatcaaga accatcccga cttattcgtt attccaatcc ctttgattat ggatgggaaa
     9481 atttataacg aaggaatcga tatcgaagcg gatgaatatt atggactttt aaaaaacagt
     9541 aaagattttc ctactacttc acaacctgtc gtaggggaag ttttgggttt atatgaggaa
     9601 ttgaaagaga aagggtatga cacgatttta agtatccatc tttcttcagg gatttctggc
     9661 tttgtcaaca cattatttgc gatgaaagat gacatcagcg gagtaacggt cattccttat
     9721 gactctaaga tcactagtat gccgatggga cacatggtag aagctgcctt agacttaaat
     9781 gatgaaggtc agtcccttga tgaaatactt gcacatatcg atcgaatccg agataatacc
     9841 tacgcttatc tgattgtaga tgatttgaac aatttagtac gaggaggacg tttgacaaat
     9901 ggtgcagcgc tgattggcag cttattaaaa atcaaaccga tcctgacatt ttctgaaggg
     9961 aaaattgttt tattcgaaaa aatccgttca agtaaaaaag catttgctcg agctgaagaa
    10021 attattggta gaagaaataa cgaaatacaa ccgccagtca aactttacgt gatacatgca
    10081 aataatttag aagtagcgat tgcggagaaa gagaaattgc agaaaaaata tccaaatgca
    10141 gaaatcgaaa ttggtcattt cggaccagtc attggtacac atttaggtga aaaagccatc
    10201 ggcattgcca tttcagcgca ataattcctt ttattcaaaa aacataccaa aaatattcca
    10261 acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt
    10321 ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc
    10381 gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat
    10441 gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag
    10501 aagactatga taaagttgat ggaagaaatg cattaaatac ggatgcaaaa gggctttcac
    10561 tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac
    10621 atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt
    10681 tagcgatctt agctgcacaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat
    10741 tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg
    10801 tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac
    10861 tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg
    10921 agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc
    10981 actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa
    11041 aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca
    11101 agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac
    11161 acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt
    11221 tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa
    11281 ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact
    11341 gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga
    11401 gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc
    11461 tgaaatcgtt cgagcgattg aaaaaggata tttattagag attcaagagc cgactgtcat
    11521 ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa
    11581 tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg
    11641 aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga
    11701 aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat
    11761 ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc
    11821 aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac
    11881 ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc
    11941 cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact
    12001 ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa
    12061 aggaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga
    12121 aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga
    12181 gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag
    12241 tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat
    12301 gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc
    12361 gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc
    12421 tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga
    12481 atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa
    12541 aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa
    12601 ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga
    12661 tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc
    12721 cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga
    12781 ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca
    12841 atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc
    12901 acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa
    12961 tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga
    13021 tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat
    13081 tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac
    13141 tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa
    13201 taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa
    13261 caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac
    13321 gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct
    13381 agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt
    13441 aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca
    13501 tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg
    13561 atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg
    13621 gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct
    13681 aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg
    13741 aataattgga catcgatttt cggcggttct gcttgggagt attctcatga aactggacag
    13801 tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg
    13861 aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt
    13921 ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct
    13981 ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt
    14041 tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga
    14101 ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat
    14161 atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaact tgcattaagc
    14221 gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat
    14281 gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca
    14341 aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag
    14401 gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg
    14461 aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc
    14521 attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat
    14581 gctggttttt ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat
    14641 gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc
    14701 attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat
    14761 ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac
    14821 ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta
    14881 ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa
    14941 gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt
    15001 gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc
    15061 accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa
    15121 acgaggtctg acaggtactt ggacatgttt gatatgcata ccgattgatg tgtcaccgat
    15181 atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa
    15241 agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa
    15301 attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc
    15361 ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca
    15421 ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct
    15481 gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac
    15541 tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct
    15601 ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg
    15661 caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg
    15721 gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta
    15781 ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc
    15841 cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat
    15901 cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag
    15961 cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga
    16021 tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt
    16081 gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta
    16141 tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct
    16201 actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca
    16261 caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga
    16321 tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc
    16381 acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac
    16441 gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc
    16501 gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct
    16561 tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt
    16621 atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca
    16681 tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata
    16741 gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt
    16801 ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat
    16861 ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt
    16921 ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt
    16981 aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac
    17041 ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc
    17101 gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc
    17161 tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga
    17221 gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa
    17281 aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc
    17341 aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca
    17401 taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac
    17461 agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc
    17521 cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa
    17581 ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga
    17641 agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt
    17701 atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta
    17761 gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga
    17821 gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat
    17881 cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga
    17941 aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc
    18001 catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta
    18061 tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt
    18121 tgttccatta aataaagaat aggaataccc gtggtgctag ttaattttaa tatcctaaag
    18181 aatatctcaa agttccttca aaacgtcgtt gtgctcgact taatagaaca ctgtaagcca
    18241 gtaatatgct ttcaaatcta ctctctagcc ctttaacaga acgtgagttg aaattttgac
    18301 aacctaattt ttctaaggaa gaaaaaacag tctcaactgt ttttctttgt tttccaagta
    18361 agctgtggtc tagtttttct gatttatcca tgtttttacg tggcggaacg gatatagtaa
    18421 tgcccttaag ggcaagcttt tcatggattt tatggctaat atatcctttg tcccctagga
    18481 tattgggaag attcgcttct tccgataaag tctctaacac tttcacgtca tggacacctg
    18541 gattcgtgat tgagtaagta atcggaaagc cagtcttagt aacgatcata tgaattttaa
    18601 gaccataaaa ataagatttt tttgttgagt tatagccaac tttggctatt tgattcaata
    18661 gttttgcttg tctatttcta actggtttac ataatggact aggaaaacta tctataattc
    18721 cgactagttc acctttggtg agcttcttga tgaaaaagta tcgaataatc ttgatagtgt
    18781 aagctaagtt tgaacttaag cgagtgaatc gactcctact aggaaagtca ccattaggaa
    18841 ataagacgga acaaaccgct ctatacgtgg ctctttggct agtatagcca ttaattatgc
    18901 cccatataac acaagcaata atcactgtat cgcgttgctt cagttgatca gtatttcttc
    18961 ggtttttaat cttatctggt acacaattgt ggtatatgtt agaaacagtt ctcataattt
    19021 ccttgaatgt tataaaagta tctgtataat gttcagtata ttttgatgaa cgcatatgta
    19081 gtcctcattt cttgggagtt ttgagactac tatatgcgtt tttttgttga tttaccaaca
    19141 tgtttagaca ttttcctcta ggaaaaaatt aactagcacc acgggtaaat acagtattat
    19201 ttcggttgct tgctgcttca cctagccctt gtactcgtgt ttgattggcg acttgaccaa
    19261 attgattccc gacgtctaat aaaataatag ctaaaagcaa aatggccaaa tggcttccgc
    19321 caaccaataa agcaataaag cttattgttt gcataacaag tcccatatac acgatttttc
    19381 tttcagaata agaatctgcc agtcgtccca cgataggtgc agcaactgct ccagacaaac
    19441 caaaaatcgc taatatccct gcttcgaatg ttccccaatt gtaggcggga ctgctgatat
    19501 aaaaaatcaa cgtagaccaa aagatcgaaa atgttccaaa catgaaaaat ccactgactg
    19561 ccgcttctct taatagacgt tgagaagcaa ctaaatgcgg caaactttta agtgaatcaa
    19621 ggtatgaaac agctgtacta ctttgctgga cagaacgcgg cattttccag taaagcagaa
    19681 gtgtaagaat tccgactgca cataaagcaa ataaatagat catccgccaa gaaaaaagac
    19741 tggcaacaat accagatacc gtccgagaaa gcaaaatccc cacgagtaaa ccgctaagca
    19801 atgtacccat gaccttccct cttgcttttg gtcccgctag taccgcaccg taaggaatga
    19861 tgatttgcgg cacgatcgaa agtaatccta ttaaaaaaga agcgcatgca aataacgtaa
    19921 acgaaggcgc aaaaaaagcg gccaacagag agacggcaga taacgctacc attcgaatga
    19981 tcaattttgg acgatccacc acgtccccta aaggaactaa aaacagtaac cctaacgcat
    20041 agcctaattg ggtcaacatc gtcaatatcc cgactacact tgtgctaaca gataaactag
    20101 ctgctacttt tgttccaatc ggctggatgt aatacatatt ggcaacaaca acgccacaag
    20161 tgattgataa tagtaaaata agcgaagttg ttaaaggtgt tttttcatta ctcttcattg
    20221 atttccccca tttcctatct tagtttacaa cttatggaaa agattggaaa tagaaaaata
    20281 caactattcg ccacacttat tatttgtgct tttgttttcc ttgttttttc ttgcgatctt
    20341 tataaatgaa gacaactaac cctactagca cagtaacact tgcaaccaac gtgactgcag
    20401 ttttgttata tccgtgaaac agagcttctc caccacacgc atacaacaca gacgaaggga
    20461 tgactccaat cacgatcgac aatatgattt gctgtagagg caatttcatc gcatctgcag
    20521 caaaattgac catagaagat ggaataaccg gtatcatata gcctaacatt acacctattt
    20581 ttggatgttt catttgtcgg attgcttgca cccaatgatt cgtttcggtt ttcttatcta
    20641 aaaattttaa gcgatgcata aggaaaattg cagtgatgtt accaagagca ttacccaaaa
    20701 tgttgatgat cgcacctgtc agtggtccaa agctcaaacc agcaatgact ccaactactg
    20761 aagtaggaat acctggaaca gcacacatca ccgctgtcaa tacgatcaag agtatcgatg
    20821 caaaagtccc atgcgaacga atatgtgcga gtagctctgc cttactcgct ctcggattta
    20881 aaaataattg gatgtctgtc tgatattcta aatacaaccg atagcccact accagtatca
    20941 gaatgattcc caacgagatc aacatcaatc ggattatttt ctttttcata tttcctcctg
    21001 aaaaaaatct atattcatta aataatagtt taacataaaa acaaaaaagc accgaaatcc
    21061 aacataggat ctcagtgctt ttaaatagta aaaattattt aacagttaca gatgcgccaa
    21121 cttcttcaag tttagctttc aattcttcag cttcttcttt agatacgcct tctttaactg
    21181 gtgcaggagc gccatcaact acagctttag cttctttcaa gcctaaacca gttgcttcac
    21241 gaactgcttt gataacttta actttttggt ctccagctgc agttaattct acagtgaatt
    21301 ctgtttgttc ttcagcagca gcagcgccac cagcagcagc tacagctaca ggagcagcag
    21361 cagatacgcc aaattcttct tcaatagctt taactaggtc gttaagttct aggatagttg
    21421 cttcttttaa ctcagcaacg atgttttcaa tgttcaatgc cattataatt tcctccgttt
    21481 aattgttctt ttttatttaa tgctttatgc ggcaattaag ctgcatctcc atcttctttg
    21541 tctgccactg ctttgacagc gtaagccacg ttgcggactg gcgcttgtag tacagatagc
    21601 aacatagata gaagtccttc gcgatttggc aattttgcca atgctgtgat ttcttccaat
    21661 gaagagactt taccttcgat gataccgcct ttgatttcta atgcttttgc ttctttagca
    21721 aattcgtcca tgattttcgc tggtgcgact acatcttcgt tactgaatgc aacagctgta
    21781 ggtccagtga atacttcgtc catgccttca aggccagctt ttttagctgc acgtgaaaga
    21841 atagaatttt tgataacttt catttctacg ttagcatcac gaagttgttt acgtaaacgt
    21901 gttacttcat caactgttaa tccgcggtag tcaacgacta ctactgatgc agctgctgta
    21961 aatttttcgc ttacttcatc aacgattgct gctttttttg cgataattgc ttcactcatt
    22021 tttgagtttc acctcctgaa ttttgatgga agagtttttt gcactgaaaa ggcaagtcac
    22081 ttttatagtt actttgctca accagtatta ttcatcacct attcacttcg ttcatagtga
    22141 ttctactcgc tttgctcgaa aggaaactcc tttcgctttg ctcaaccagt attgttcatc
    22201 atctcttcac ttcgttcata gtgattcaca ataaaaaact ctatgccacc gcagacatag
    22261 agggactatt gaatatattc aataaacgtc ctcggtagga aattaaggct tgatgccacc
    22321 tactgtcttc ggtacaagtt aattattaac ctcaactagc ttagcatgct aaactagttg
    22381 agtcaagaat tatttttcag ttggatcaat caacattcta atatttaatt agaaagaagc
    22441 ttggtcaaca tggataccag gtccaaatgt tgttgtgatc gttacatttt tcatgtattg
    22501 acctttagct gctgatggtt tagcttttag caatacatca tggattgctg caaagttttc
    22561 aactaatttt tcatcgtcaa atgaaacttt tccgattggt acatggatat ttccagcttt
    22621 gtccacacgg taagttactt taccagcttt cacttcgttt actgcttttg ttacgtccat
    22681 tgtaactgta ccagttttag ggtttggcat taagcctttt ggtcctaata ctcgtcctag
    22741 acgacctaca gtagccatca tatcaggtgt tgcaacaact acatcaaagt caaaccatcc
    22801 gttttggatt ttttgaacca tgtcgtcgtc acctacgaaa tctgcaccag ctgcttcagc
    22861 ttcttttgct ttgtctcctt tagcaaaaac taaaacagtt tgtgttttac cagttccgtt
    22921 tggtaataca acagcaccac ggatttgttg gtctgctttt ttagggtcta cgttcaaacg
    22981 gtatgcaact tcaactgttg catcaaattt tgcaatgttt gtttcttttg ctaaagctac
    23041 tgcttcggca acaggataaa ctttacttga atcaactttt tttaatgcat cttgcatttt
    23101 tttgctcttt ttagccattt tggttcctcc ttgattgtgg ttataacggt tgtacctccc
    23161 acgtgttcta tatctttcaa tatagagcca actgagaagc tgctgtctta gggtgtatag
    23221 attattctac agtgatcccc atgcttcgtg cagttccttc aaccatgcgc attgctgctt
    23281 caacgttagc tgcgtttagg tcttccattt ttaattcagc gatttctctt acttgatcgc
    23341 tagaaacttt cgcaacttta tttttgtttg gttcgcctga acctttttcg attttagctg
    23401 cttttttcaa tagaacagca gctggcggtg tttttgtaac gaatgtgaat gaacggtctt
    23461 cgtatacaga aataacaacc ggaatgatca aacctgcttg atcagctgta cgagcgttga
    23521 attcttttgt gaatcccatg atattgatac ccgcttgacc tagtgcagga cctactggtg
    23581 gagctggagt tgctttacct gcaggaattt gcaatttaac gattttttct actttctttg
    23641 ccacgagaca tacctcctta agtccgtgat gtggttaatt ggagatgcat atttctcctc
    23701 ccacgtttta tctgatctga aaaaatccag acaaataatg tgcaaaacaa tgcacactgg
    23761 tacagtatag caaatttatt tgttttttca agattttttt ctgctatttt tacattttgc
    23821 ttgaataaac gctaaattta ttctaaaaaa acaacaattt ttcgttctca tttcttcttt
    23881 tatgttacac ttgcgctagc atggttcaac agatgaaagc aaacaataac acggtttttt
    23941 agacccttgt acaaagattc ctacataaca aaatgaggtg gagaaaatga aagatttaag
    24001 gtatagaagt gtctttgata ttattggacc tgtgatgatt ggtccaagta gctctcacac
    24061 tgccggagct gctagaatcg ggaaaatcat acgtagtata tttggcgaac agcctgattc
    24121 tgtagatatt tatctatatg aatcatttgc aaaaacctat cgggggcacg gaacagatat
    24181 tgcattagtt ggcggcctgt tagggatgga acccgatgat gaacgtttag ctgattcttt
    24241 gaaattggca tatgaagccg gtatggaagt cttgtttgtt cctaagagtg aaaaagccga
    24301 acatccaaac tctgtaaaga tgcttctaaa aaaaggaaca cgaaagcttt cagtcactgg
    24361 catttcgatc ggcggtggca atatccagat ttctgaattg aatggattca aaatttcttt
    24421 aagcatgggt actcctacct tgatcgttgt ccaccaagat gttccaggta tgattgctaa
    24481 agtgactagt atactttctg agaccaatac gaatatcggc actatgacag tgacacggga
    24541 atcaaaaggt gaaaatgcca ttatgataat tgaagtagat gatcctcaag tagaagaaac
    24601 ggtaaaaaga ctcaaacagc tgccaaatat cgacagcgta aattatttcg attaaggagc
    24661 taaaaaaatg tttttatcga ttgaagaatt agtagatcaa gcaaaagctt atccaagtgt
    24721 agcagaattg atgattgcag ttgaaatgga aatgagtggc cgttcccgta gtcacatcat
    24781 cgagatcatg gaaaaaaacc tctcagtaat ggaacaatca atcaacgagg gaaaagaagg
    24841 tgttacctcc gtcacaggaa ttaccggtgg tgatgcaaaa aaattagatg attatttaaa
    24901 tgaggcagac tttttaagtg gagaaacaat cttaaaagct gtacgcaatg ctgtagctgt
    24961 taatgaagtc aatgcaaaaa tggggttgat ttgtgctaca ccgactgccg gaagcgcggg
    25021 tgttgttcct ggtgtgctga tggctgtccg agatcggctt gatttgacac atgaacagca
    25081 attggaattc ttatttaccg ctggcgcttt cgggttagtc atcgcaaaca acgcttctat
    25141 cagtggtgca gaagggggat gtcaagcaga agttggttct gcaagtgcaa tggcagcagc
    25201 agctttggtt tgtgcaaaaa atgggactcc tcatcaagca gcacaagcag tagccattac
    25261 attgaaaaat atgatgggac tgatttgcga tcccgttgca ggcttagttg aagtcccgtg
    25321 tgtcaaacgt aatgctttag gctcttctca agcttttatc tctgctgata tggcattagc
    25381 gggtatcgaa agtgtcattc cgccggatga agtcgttgct gcaatgtacc aagtcggacg
    25441 tcaaatgccg tcgattttca aagaaacagc agaaggtggc ttagctgtta cgccaactgc
    25501 taaacgactt gcggaagaga ttttaaataa gagggcgtga taaagctcct taatttcgag
    25561 tattaagaga ttagttatct tcataacaaa aaacgaagag aggactaatt gcccttctct
    25621 tcgtttttta ttaatccatc aatttcgtat tttttacttc atcgatcgtt tttgttcctg
    25681 caagttgcat cgtgatcgac aattctttgt tcaggtgttc aaaaacagat gttactcctt
    25741 ctgccccacc tagattcaac ccataaatca ctggtcggcc aatcgcaact aagtcagcac
    25801 cacttgccag agctttgaag acatgctctc ctcgacggac accagagtcg aaaatcactg
    25861 gtacacgttt gttgactact tcggcaattt ttggcaatac ttcaaaggac gcaggacctc
    25921 catctagttg acgtccacca tgattggata cccagatacc atctgcccct gcagagatag
    25981 cgattactgc atcttctggc gattgtattc cttttacgat aactggaaga tgggtgattt
    26041 ctttgattgt tttgatgtca tctggtgtca agccttgttt tgcagctgca taaatttctg
    26101 cgattccttt tccttcgcca ttaccacttg cactttgttc gctatacgca gctaagttag
    26161 gcattggtaa tggaaattgg aattgattga tgacgtcttc ttcacggtaa ccaccgagcg
    26221 ttgaatctgc cgttaaaatg atagctttag cccctgcttc tactgctttg ttcaaaatga
    26281 actcattgaa tctgtcgtct ttactcatat ataattggaa aaactgcggt gcgcctggcg
    26341 ctgcatcggc tgcgtctttg attgtcgtat ttgcataagt gctgatggaa aagattgatc
    26401 cagcagcagc tactccttta gctgtatcag cttccccttt ttcatgtgcc aatccttggg
    26461 ccgctgatgg cgcttggata atcggtgtat ctaattcaat tccgaaaaca gatgtatgca
    26521 aatcggctga gtcgattccg cgtaatatcc gtggcatgat tttctttgta ttaaaagaag
    26581 tcgtattttc tttcatcgtc cactcatctt cagaaccacc gcgaatatag ccaaaagctc
    26641 ctgcttccat gcgctctttt acacgacctt ctagttaggc aatgtttaca atatcgattg
    26701 gatgttcttc attacttgta tgataagtca tagtagatcc ttctttcatc ttcttgatta
    26761 catataaatc atactaaact tacaaaaagt aagcaaacat ttactctcag atacttttat
    26821 cgctattctt tccaaataaa tcgacttaca agctgatcta cttcttggtt ttcatgaagc
    26881 atactatgag aggcattttc acctgtcact actttttcct ctatttcatt tccgcgttgc
    26941 ttcaacaacg catagaccgc taatgcactg tttaacggta ctgttccatc actcaagtct
    27001 gtttcatcta gttgaccagc aatcaagaaa aatcgagtgg ttataggaat attaccgatc
    27061 atttgctggt aatcttgata gcggttagaa acgacagcag gcccctttgc taactcatct
    27121 gtcagagact gcgcactatc atcgacaaaa tcattaaaag gcgcgccaat tgctgttaat
    27181 gtctgaactt gtggcaatga cgtatcctgt ccatacgttc ctaaatagcg tagtgcacta
    27241 actcctccca tggaatgccc cactatgttg actttatcta tgccgtagtt ttctttcaga
    27301 tacaaaagac aagttttgat ccattctgct tgattccact cgttattttt attatcttca
    27361 aacaatactt ggaccatcgg attgtctgct tttccagaca aatctcctgt tgcttgtatt
    27421 tgtccatctg ttcctactgt taaaaccaat tcttttactg cttgctgatt tttttccaaa
    27481 cgccgcaaca tccctttaaa ggaattttct gtcccgctgt agccgtggaa aaaaagtgtt
    27541 ggaatagtgg ttttttctat cactttactg gattctgccg ttttttcttt atcagaaact
    27601 tcagtagtat ttatcttcgt cgtctggcaa ccacttaacg caaacaaact gataaggact
    27661 aaagctgctg cagttgcacg tttgcataaa tttttgattg gttctgtcat acacctcttc
    27721 ctttccatat agaaaaaaga aagacttgaa aggccaatca tatgatcgac atttcaagtc
    27781 tctttattaa tcgattttat ctacttgatc gaaatcaagt tctgtactgg tttcacgtcc
    27841 aaacatatcg atgttgactt tcaatttttc tttttctccg tcaacttctg ttacttgtcc
    27901 ttctaatcca gcaaatgcgc cttcgatgat tttcacgact tcgccaactt ctacttctag
    27961 atcattttgt cgcaggctca taccgataga acgcaagata tgattgactt cttctggaag
    28021 taatggtgct ggtttgcttc cggctccgtg tgaaccaacg aatcctgtca caccaggtgt
    28081 gttgcggaca acataccatg aagcgtctgt catgatcatt tcaacaagta cgtatcctgg
    28141 gaatgtttta tgaacgattt ctttttcttt cccatttttg acttcagttt ctttttcttc
    28201 aggaacaacg acacggaaaa taaagtcggc cattttcata ctttgcgcac gagattcgat
    28261 atttgctttt actttgtttt catatcctga atatgtatgc aagacatacc agtttttttc
    28321 aaaagattcc attgaaggct cctcatttca ttgtaaaata aaaaaacctc agtacacccg
    28381 aagtttttct ttcgcttcat tatagcacta aaagtacgtt gtggctagcg agttatttta
    28441 aaatccagcc aaataagctt tggatggctg tatccattac gtaaaacatc aatccgaaaa
    28501 taatagaagt ttcgatgact actaacgtat ctttacggag ttgttttctg cttggccaag
    28561 ttacttgttt gatttcatct tttacgcttt ttagaaattt catgagacaa cctcccttgc
    28621 tactgtttat ttcgtttctt tatgtaatgt atattgatta caatatttac aaaacttatt
    28681 gatttccaaa cgttcgccgc tttttccttc actgactgat tttgtgtagt tgcgagaccc
    28741 acaaacggaa caagcgagtg ctgctttttt ctttgccatt tacttctgcc cccttgctga
    28801 gaatcagact tatacttcat aacagtatca cgtaactttt tacttgtcaa tttgtgaaat
    28861 aaactatcta ttgttttgga taaaattatg atatgattaa tttgaaatta agctaaaatg
    28921 agacaattgg aggtatagcg atgctattaa aaacacttgt atataagaaa aaagatctga
    28981 caaccgttac agagactagt acattagaag aagcattaaa aattttagaa acttccggat
    29041 accgttgtgt gccgatctta gattcttcag gtaatatttt ccgtggaaac atttacaaaa
    29101 tgcatatcta tcgtcataaa gcaaatggcg gtgatatgac gcttcctgtc actcatcttt
    29161 tgaaaaacgc tacaaagttc atttacctgg atacttcttt cttcaaagta ttctttacga
    29221 tcaaagagct tccttatatt gctgtccttg acagtgaaaa tcatttttat ggtattttga
    29281 cacatagtac gttgcttaat atgctctctc aatcttggag cgtagaacaa ggaagctatg
    29341 tactgacaat tgcttccaca ggaaagcaag gagatttggc tgctattgca aaaatcattt
    29401 caaaacacag cagcattgcc agctgtatta ctcttgatat cgaaaaagac gaatacattc
    29461 gacgtacatt gatcacgcta ccggctaaca ctgaagcgaa agtatgtgaa acgatcgtca
    29521 accacttaga gaaaaagaat tataaagtgg tagaaattga aaatttgcaa aaccaagaag
    29581 cataatcaaa aagcgaaaaa agaaaacagc taatctgctt tcttttttcg ctttttataa
    29641 ttagacacgc ttttcaaaat caagcgcgcg atctaaataa tacatcaatg gtgctgaaac
    29701 agccatgacg ataaactcac ttaatgcaag cgtgccatag gttgtccaaa atgcttggcc
    29761 acctgttgga actaacatat aagcaatcag tcccatgctg atggtaaaga agatcgtgtt
    29821 caatactaaa cgcatcttta catttttcac tttgttttgc aataaagctg tcaggcctaa
    29881 agaaagcaat gtttggccgc caccaaaaac cacatccatg ataccaaaac caaagaaggc
    29941 attatagacg atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa agacaactaa
    30001 atgattcaaa ctttctgaaa tacgaaactg gatcggccct gaggatacag gcgcaactaa
    30061 aatagttaac gctagataaa gagccatgat gatgccattt acaacgatta cggataaccg
    30121 ttgtctgctt ttcgtttttt gcattttctc tcctcttttc tcctagtttt tttaacgcgg
    30181 gatggttgat gaaccgcggt tatgtgaaac aaacaataat tataacagac ttttcattta
    30241 tttcaatccg tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta aacgtttgct
    30301 aaaaatcgtg ggatatggta taaagtaagt gtaaggggtc ataagaattc ccagtaaagc
    30361 agacgaagta ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat aaaaaaggat
    30421 gtgggaattt gagcaacctc ttactagaga acggtctagc aagcagctga agatctaagt
    30481 ggacatgccg aaccttggaa cttctcctta cgtaagtaat ggatcatgcg cttgctaggc
    30541 ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg ctataataac
    30601 caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat tcaaaaaata
    30661 gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata tcttgtttta
    30721 ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat ccatcggcag
    30781 gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa aataagcata
    30841 ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt gctgaaactt
    30901 tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc gatactttcc
    30961 cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat actttctatc
    31021 agatggaagt agaattccca gttgcttata ttccagcagg ttctggaaat gattttgctc
    31081 gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta gcagctcaat
    31141 caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag
    31201 ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca aatcattctt
    31261 ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta ttctctattc
    31321 tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga ggaaaacaat
    31381 taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt ggcggaggaa
    31441 tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac
    31501 gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa aaacaattga
    31561 aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca acaacaccgc
    31621 aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc acaataacca
    31681 caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta catctggaac
    31741 accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt tttttactat
    31801 gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta cccaaaataa
    31861 attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg aaccaaaaag
    31921 attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta ttttgaacaa
    31981 accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag tattctgctg
    32041 gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg atcccaccgc
    32101 tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg atcggcaata
    32161 aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag gtgtggatca
    32221 ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa ctaatcaaac
    32281 taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg tttcgtttac
    32341 tgatctcttt gcgattaaac gctagattga tgacgctgaa accgctaaaa atggatagaa
    32401 acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc atccgctgac
    32461 tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt gtctgttcca
    32521 tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt
    32581 tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt atcttgatcg
    32641 tcgccatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt aagataatgt
    32701 ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca tccaatgtaa
    32761 cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc gactcttctt
    32821 cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca atattttgcg
    32881 caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc gatttatttg
    32941 ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct ttccatccaa
    33001 gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt ggtataaaaa
    33061 ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa gttgacgaga
    33121 tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg ttccgacccc
    33181 atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct atccttttgc
    33241 accagattca tttttggtat caaattacca accaatgtac ctaacaaaat cgcacacaac
    33301 gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc tacttgatag
    33361 ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt aaagcttgct
    33421 gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa gcgaatacca
    33481 tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat cgtcccgtag
    33541 agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat attacgacct
    33601 gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg cgcgagatca
    33661 acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa aaattgatcc
    33721 atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag cgaaacagat
    33781 gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc attcgcttct
    33841 acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac ttgttctaag
    33901 actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat tccagtatta
    33961 atcgtttcaa gattgccacc ttgatcaatc tcgccaaagg ccgccataaa catcaatcct
    34021 aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag cagtattttt
    34081 gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc tcaaatcctt
    34141 gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta tttttccgta
    34201 actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat ttagttcagt
    34261 agttgttact tggagctgac cttttttata agcgattcca tcagaagcta ttgttcgctg
    34321 aatcgctgac aacacatttt ctgggagtaa tgggatgtcg atcgtcaaac gatccgccgt
    34381 ccgtaccata tcttttagag attctttggt tcctttagca atcacctgtc cttgatccat
    34441 gatcacgatc tggtcgcaaa gaatttctac ttcttccatg taatgtgttg tatagacaat
    34501 ggttgctcct tgtcgattca gctccttgat actttctaaa attttattcc gactttgggg
    34561 gtctacagct actgtcggct catctaaaaa aattaaccgc ggcttgtggg caatcccaca
    34621 tgcgatgttc aaccgacgtt tcaagccacc actaagttct cctggacgaa acgaaacata
    34681 tttttctaat cccactaatt gaatcacctc ttcaatcaag gctttccttt tctctttttc
    34741 ggaaacatat aaaccgcaaa aataatcgat attttcttgc acacttagtt cgtcaaagac
    34801 cgcaacgtct tgaggaacga ccccaatcat tgccttgaca gcataatttt caggagtcat
    34861 tgttgtcccg aacaatttaa tggttccttt gtcataattt aacaaagaaa gcatacagtt
    34921 gatggcagtc gatttcccac tgccgtttgg acctaacaga cctaagactt ctccttcttg
    34981 tacttgcaga tcaaaatgat tcaatgctac aaaatctcca tagcgtttga ctaatttatc
    35041 aatttcgata atcatcatga aaaactcctt ccaaatttct tgttgatctg actataataa
    35101 agtgcaagaa aagaaactag tgacgattgg ctaaaccaca catgacattt gtcacaatga
    35161 aggagtctgc tatgcataaa tctcggctac tcaacgaaac tagctttcta ctggttatct
    35221 cattgctttt gctggtaagt caaggattcg ttgctatgca agttatctat ttattgatcg
    35281 ccgttttatt cactggtgtc agttatttac tgcctacaaa gccaaattgg attttccaag
    35341 ttattctatt tcttttatgt gcctttttac ctgatcttgt cctgtttctg ccagcgacga
    35401 tgcgagtgat cgcccctgaa gaaagccacc ttgattactt gtttttgtta ggttttttgt
    35461 tgatttcgac ggttggtaat catcccccca tcaaggtcgc ctttgtcgta attttgctgt
    35521 ttttttcctt ttacttacga aaaaaagatc aacgaacgac cgatcttgag aaaaagtaca
    35581 ttgtctccaa agatactcat tgggaaaaac aacaagagtt atcggaacaa aataaagcat
    35641 tggccctgtc acaagaaaca caattagcct tgcaaattgt ggaagaacgg aaccgaattg
    35701 cccgagacat ccatgacaat gtcggtcatc tgctgtcaag tgcgatttta caaattggtg
    35761 cgcttgaaat gatcaatcaa gaaccaaaac taaaagaacc attagaaaat ttaagtgcca
    35821 ccgttcacac tgggatggat cggattcgtc aaagtgtcca tgacctccat gagacatcga
    35881 tctcttttca acactcctta gagtttctga tcgctgattt tccttcttct gttacgattg
    35941 aaggaaactt gtttgaagga ttgaatgaaa cgcagcaaaa ctgcttctta atggtcatca
    36001 aagaagcctt aaccaacagt atcaaacata gtcatgccaa aaccgttgtt ttacattttc
    36061 gaacgttgcc ggccttttat cgcttgcaaa tcacgaatga tggcaccagc ccacaaaaaa
    36121 attctgctcc cggtattggt ttgaaaacga tgcgtcaacg tgttcaagaa caaaagggac
    36181 aagtccatat tttccaagaa aaccagcaat ttcagttaac gatcatttta ccaagggagg
    36241 gacaccaatg ataacagtag tcattattga tgatgatcca tttgtgacga aatcgttaca
    36301 aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt gtgccaagga
    36361 tgcgcgaaac ccatcgtccc gatgttttgc tgacggacat ccgcatgcct aaacaaacgg
    36421 gcatcgatgc tgccaaagaa attttggcta aatttcccaa agccatcctt ttgcttttga
    36481 cgacattcaa agatgaagaa tacatccgcg aagcattttc catcggtgtc aaagggtatt
    36541 taatcaaaca gaacttgcaa gcgattattc cttctgtcaa atccgcttat aatggacagg
    36601 ccgtatttgg taatgaaatc atcgagactt tcactcaaat gatgaagaat aaaccgacga
    36661 ttgatagtca tgcttatgct tcttttagtg aacgtgagct tgcaatcatc aaagaagtag
    36721 ctgccgggaa aaacaataaa gaaattgcag atgccttgta cttgagtgac ggcactgttc
    36781 gcaattacat cagccaatta ctagaaaagc tagatttacg ggatcgcacg caattggcta
    36841 tctattttta tcagcatttg caataagtct tatctaacac aaaaaaacct gaatgatgct
    36901 ccttcgcatc attcaggttt ttttcacatc ttctattcaa ttgtcccgca aaccatttcc
    36961 tcacgattga aagaatcaag gattcgcagg gctgccagga ttcgttggat tgttcggcgt
    37021 gttaggcgga aaaataggcg ccttgtctaa cgttttttct agttttgatt cttcacgatc
    37081 gtctcgttct acatcaggac tttcattcaa tttgaattct ttttccatct catctaactt
    37141 tttaaagtca ttgccagatt cttttgctaa tttcgctaat ttatcttcgg tttctttagc
    37201 cataaagtaa cgcccccctt ttgatttcag cttagcatat cacactgaaa aaacaaaaga
    37261 aaacgctaac caacgatcat ttttagctat ttctcatctt taaaatcaag cattttttgc
    37321 cggtataaaa tcgtagggag aactgttttc cataccaatc atcggatcta ctaactggtt
    37381 atcgatcact tctgctgtaa gaacgaaatc tttagaaaca gcaggttcta cctcttcttt
    37441 cttttcttct ccaacaaatg aagaaaacag atctggttcc actgtttcca ttacttggtt
    37501 tttcgtttca ttcgtcccac tgtcatcttc tacaggtagc ttttctttta caacccgtgg
    37561 ctcttttgta tagttcccat cttcaaatgg atcatttccg accaaagaat cttcataagc
    37621 tgctagtttg gtccggagag tgatattcat tttatcatta tccatgatcc ttgatttcaa
    37681 cgtagtcatc cggcttgctg attcattcgc tacacaagtg acataggctt gctcttcccc
    37741 taacaaaatc aacagttcct tactgcgagt cactgccgtg tagagcaaat tacgctgaag
    37801 catccgctga tattgatgga ccatgggcaa aataaccatc ttgaattcac tgccttgtga
    37861 tttatggatt gagcaacaat aagaaagagt gattttattc cattcgtttc ttttataagt
    37921 gacctcattt gcatcaaact ggataactaa ttcatccact ttatcttcag aatcttttgc
    37981 caagatgata cccgtgattt ctcccatatc tccattgaat acattcaatt cgggagtatt
    38041 gactaattgg aggactttgt ctcctatccg ataaacggtg tcgttccaat gtacttcctt
    38101 ttttccaggt ttccctggat tgaatatctc ctgcatcatt ttatttaacg catcgatccc
    38161 tgcaggtccc cgatacattg gtgctaatac ttggatatct tgtgcacgga atcccttatt
    38221 tttcgctttt tccacaattt tagagatcaa gggttcaatt tgataagcat cacttcggaa
    38281 ataggaacga tctttttgat tctttgtgaa atcttttggc agacgtcctt ctttgatctc
    38341 atgggcaaga ggaataatac tagaaccatc accttgccga taaatctgat tgagttccat
    38401 tttgggaatt tgttcgattt ccaaaagatc atggagtact tgaccaggac cgacagaggg
    38461 aagctgatct ttgtctccta ctaagataac ttgcatattc gtcggaatcg ctttgaataa
    38521 tgtgttagcc agccatgtat cgaccataga catctcatca acaatcaaaa gtccgccttc
    38581 taattctttc gtactgactg aaggtgtttt ttcccgtcca ttcagcccca gaagacgatg
    38641 gatcgtactg gcaggcagac ctgtcgtttc gttcattcgt ttagctgctc ggccagttgg
    38701 tgctgcaagt agaataggaa aaatttcttg ggtatatttt gaaggatcca attccagtcc
    38761 gttcaactct gcaaaaagat tgacgattcc gttgatcaca gtagttttcc ccgtacctgg
    38821 tcctcctgtt aaaataaata gaggggaacg gattgcttca gcgatagctt gttcttgcga
    38881 aggtccatac tgtatgccga atattttttc gatacgccga atattcttct gcacttcttt
    38941 ttcaggataa tggatttctt ttttacgact gagaaggcgc tggatcgaat taccgatccc
    39001 ccattcagaa aagtacaaac tgttttcgta aagattagtc tcttcttgtt gtatctttcc
    39061 ttcttcaaca agttggataa ttacagtagc aacctgatca ggctggattt caattgggcg
    39121 acttgtttct aaaatacgga tcgtttgctg aagcaatgtc tccgccggta cgtatgtatc
    39181 tcctgtttga actgcatgct gaaaaatctg atgcaagata gctgctcgaa ttcttctcgg
    39241 tgaatctgct gcaataccga tttgttcggc tagattatct gcacgtttga acccgattcc
    39301 ttctatgtct tcgactagct ggtacggatt ttcttcgatg atatccaaag actcgttctt
    39361 ataggcctgg tagatactga aagctaactg actaccgaac ccgtaacgat tcagtcccat
    39421 gatcacttga tccatcccgt ggtttagccg aatcgtctca gcaagcattt ctctttttgg
    39481 cttcgtcaaa ccggagattt gttttaatac cgaaggatca gcaatgattt tttcgatagc
    39541 atcttctccc agcaattcta caattttttc tgctgttttc ttcccgattc ctgggaattt
    39601 ttcgctggaa agatacgcta cgatcccatt tgcagttgtt ggcttttctt gctcatatct
    39661 ttcgagttta agctgccttc cgtatttagg atgatccact aattctccat aaaaacgata
    39721 agtctcattt tcttgaatat cgccgaaact tccggtgact acaatctctt tttcacggta
    39781 atcagctgtg gtttcactga tgtgtaccaa aagcactttg taaaaattac taggattttg
    39841 aaaaaaaatc gcggcaactg taccaacgaa ataaggcatt tcttctggct gcatcggaag
    39901 ccgcctcctt cttctatagt aaaggttctg gtgctgtttc agttcctaaa aaatctgttt
    39961 ggttccatac tttcagttca tatggcaata atctgcaagg ctcttcagtc tctaaaatcg
    40021 tcaaactact atttaacaat ccacccattt cacgcaactc acttagctct tttcctgtca
    40081 tccattgaat ggctgcagtt aaagaagctc catgtcctac gaataaaagt gggccatctt
    40141 tatgtcgggc aaccgcatct gctacaacag tttcgatacg tgtgattgcc gattcaatcg
    40201 tttctccgtg aaaaggagaa ggatcataca gatctaaatg attacgcaaa ttcggcagat
    40261 tatccggata tttctggtac atttcatcga tcgactgacc ttctaaagta cccagcccta
    40321 gttctcttag cttatctgta taaaaaatct ctaccggacg agcaagctct ttgttgatac
    40381 cttctgctgt atcacgtgct cgctttgctg tactggaata aattgcttta aaaggaacat
    40441 attttacctt tttcccaaat gctttgattt cttcgtaact ttgtggcaaa agcggagaat
    40501 cgccattgct cccttgaaaa cgtctttcct gattccattg tgtcttccca tgtcgggtaa
    40561 aatatagttc catacgcagc aaactcctgt ctttctttta ttttactcta taataaactg
    40621 tctaatccag cttgtcattt tcatagtgat gcgtcaattc taaattaggg aatccttgct
    40681 ggcgaagtgc ttcatacact atcattgcag ctgtattcga taaattcaat gagcggacat
    40741 gctcatcatt catagggatg cgtaaacact tttcttcgtt ttcacgcata aatgtttctg
    40801 gcaaaccggt tgtttctttt ccaaataaaa agaagtgctc ttctccatca gcaaaatttt
    40861 cgtcactata tactttatgg gcaaattttg agatcaaatg tagtttacga ttacctaaaa
    40921 atgttaaaaa agcttctaaa ttactgtggt aagtaatatt tacatcattc caataatcca
    40981 accctgcccg ttttagatgt ttgtcatcag ttgaaaaacc tagtggctcg attaaatgaa
    41041 gcggagtatt cgtggctgca cacgtacggg caatatttcc agtgtttgca gggattaatg
    41101 gttcaaataa aacgatatga ttcatcagga tcattcctct catatttatg ttcgtttcta
    41161 ttttctgaca atttgatttt tgcataaaaa aaaacgtatc aactcggtgt cagtcactgc
    41221 gagtcgttac gttagtgaag ctcttctcac taacttttat cagaacagga acctgataaa
    41281 aaccaatgaa aaaacaactt gttgctaata taacaccctc aacttttttt tgcaacctct
    41341 actcattaaa aactgctaga aaaacgtttt ctcttagttc cttttaataa ttctatgtca
    41401 attgtaatct tcttcaaagg attcttatat aagtctgctt tgattcgctc ttctacctgc
    41461 cattctaaag gtgacatggc caaaagatct ttttgatttt cctctggtat ctcaaattca
    41521 taggttacac gctgacgttc agttaatttc attgcttcag caaacttggc tactacacgt
    41581 tcattggaat aactctgttt ttcaggttga ttcggataaa aagcctgacg tagttccttt
    41641 aaataatttt cttgtggcac caccttcaac acttctccat cttctgagag tacacgttgg
    41701 aattcttggt aatgagaagg cgaaaagata tttaaaaccg cgttgaatgt ttgatcagca
    41761 aacggcaaat tggtcaaatc agctacacac caaaatgctt cggcagattg ttctgtagct
    41821 gcgtagactc cttcttttga aatatcaaat ccaacagcat tttgaatctt tttcaatttt
    41881 gaaagttcat tcaagaagct accttctccg cagccaacat ctaatagcga gacttcttca
    41941 ggtaaatgct gcgcaatagt ctcaataacg ggctgataca tgccagattg gatcattcta
    42001 cgccttggaa cgaacattcc ttgatcgtat tctgttttaa ccgcatggga taaaaaataa
    42061 agtgttcctt ttttggaaag gtcaaaacgg tgattatttt tgcatatcaa gccattatca
    42121 gactttttta cttcttcttg gcaaaccgga cagcggaaca gctgatgatt ttctgccaaa
    42181 aactggcgtc ctcggtcaat tttttttaac atagttcccc ctcatttctg ttttatctta
    42241 ccgttagatg ctcactttgg caactgctaa aaatatgata caatgcctag aagaaagtga
    42301 aagataaagg agaagattag atgatcaaag aattttgcgc tgaaaactac acatctattc
    42361 ctctagcaat tgctagagga gccgatcgga tcgaattgtg tgacaactta gcggtcggtg
    42421 gcacaacccc gagtactggt gtgatcgaag aaacgttaag ttatgctggg gaaaaagata
    42481 tccccgtaat gacgattatt cgtcctagag ggggcgactt cgtctataac gatatcgaat
    42541 tgaaaatcat gcatactgat ttgattgaag caaagaaatt agggacggat ggcgtagtat
    42601 ttggttgtct tactccttca ggctggctag acgaagaggc cttagaacta ttgatcgaaa
    42661 cagcagaagg gttgcagatc accttccata tggcttttga cagtattcct gaagatcggc
    42721 aattcgaagc aatcgattgg ctggtagaac atggtgttga ccgtatcttg actcatggcg
    42781 gtcctgctga caaaactatt gtagaaaatt tcccacggct taaagaattg attgcctata
    42841 cagatggccg cttaatcatt ttgcctggtg gtggtgtcac tgcagagaat gcagcacatg
    42901 tagctaaaga attgaacgta agcgaagtac acggaaccaa aatcgtggat cttcagttat
    42961 aatcttataa acaaaaaaac atttgcaaca ggcataatgc ttattgcaaa tgtttttgtt
    43021 tttttacata aaatagccca taaataaaga agcaatattg aaataaatca gtacactcgt
    43081 caaatcgctc aaagtcgtga taaagggacc gcttgctact gctggatcaa atcctagacg
    43141 atccattagc atcgggatta aactacctgc aagattcgcc actgtgattg caaatagcat
    43201 agccatccct atgacaaacc ccaagataaa attatgtttc caaaaaccta cgattagaaa
    43261 aatcgtgatc cccgtaacag ctcctgtgac aacgccggtc aagacttcag ccaaaacaag
    43321 tcgaacaaat ccatgatctt tctcatcaga aatcgcaagt cgacgaacag ccacagctag
    43381 cgactgggtt ccagcattac cggcagttcc tgtaatcagc gaaatgaaga ctgctaaaat
    43441 actcgcttca cttaccaagt cttcatagtg actgattaaa gaagcggtcg acatacctaa
    43501 aaacaacaga gtgatcagcc aaggcaaacg tcgagacgct gcctttactg gattttcatt
    43561 aatttcttcc acattgaccc cggcaagtcc tgaataatcg ctagcagctt catcatcaat
    43621 aacgtcgatg atatcatcga ctgtgacgat tcctaacaaa tgatcatcat aatcggtaac
    43681 aggaagagct aagaagtcat aatctctaaa tgtttgtgcc acatcttctt ggtcgtcgcc
    43741 tacatgaact gaaagcactc gttcgctcat cagatcagaa atcattgcgt cgtcatcatt
    43801 cacgattagg tcgcgcagag aaataacccc tactagctga ccttcttgat taacgacata
    43861 gatgtaatag attgtttcag ctacatctgc ttcgttcttc agcacgtaca tcgcagagcg
    43921 aacagtttga ttggctacga tcgaaacaaa ttcagttgtc atgatcgcgc cagccgtttc
    43981 atcctcgtaa tggagaagtt ccttgatttc actggcatcg tctgccgaca tcaaacttaa
    44041 atatttagca atttgctttt tatccagcgt attaagcaag tcaacagcat tatctgtata
    44101 catctcagaa agcatatcag ctgcatagct aggacgcatt tcatctaggt attctttcat
    44161 atgttcgttg tcttcttcaa tcacatcgaa catatccgct aattctttag gcgacagata
    44221 ggaatagatc agctgcctgt tctcttctgt aagagattgg tagaattgcc cctgctcata
    44281 gatatgcatc tctaaaaaac tgtttctgaa tgcctgaata ttattttccc ttaaatcttg
    44341 agtcaactgg gcaaatcttt cctctaattc ttgattttca tccaaaccaa tctcacctcg
    44401 ccttattctt caaaccgtct cacaactgcc tccatgtctg cggctaaagg ctgcttgaac
    44461 tccagcaact cttctgtaaa tggatggtaa aaacgcagat cataacaatg aagggcttgt
    44521 ctttttattc ccagatccat tcgcccgcca tacatatcat caccgagcag ggcacagcca
    44581 attgcggaaa aatgaactct tatttgatgt gtccttcctg tatgcagctg gatatcaact
    44641 aacgcgattt ctgttagtcg cttctctatc cagtactcag tctgtgccga acgtccttca
    44701 tcagagacca ttcgcttcaa tagcgaagaa taatctcggg cgatcggcaa atcgatatgt
    44761 ccatgtgccg ataaatctga aagccttcca ctgactaagg cctgatattt tttcacaaac
    44821 ttcttttcac gaagttgcat atctaatttt gcgtgcgcaa atccatgctt tgcaaaaagc
    44881 atcaagccag atgtatcccg atctagtcgc gtcaccacat ggattacttg gttttcgtaa
    44941 ccttgttttt tataataagc ctttacacga ttagccattg tcccatttgg atggtattgg
    45001 gcaggaatag acgaaacgcc tgctggttta tttactacca gaacgtgttc atcttcatac
    45061 acgatatcga tcggtgtttc atccaaaagt acggtttcat gttctccttc agcaggaatc
    45121 acaatggtta ccttatcatc cttagctaga gaaaacaaga cattttctac ttgatcatta
    45181 actttgattt gaccgccttg aaattttatt ttcgctaaca gaccctttga gatccctttt
    45241 tcttttaaaa aatattttac ttgctgagaa tgatctttct cataatccca tgtaaactgc
    45301 atatcaatcc tcactaataa aggcatcttt cacacgatgc caaaaatgca tatgtcgata
    45361 agaagcaaaa tggatccgtt cttctgctat tcgataataa attgagcgga tatttgcttt
    45421 tgcgacattc aattgatcaa ccgttacaag ataatcttct gtattttcca attttatttc
    45481 tagccattca tcatgggcaa tcacgatcgg agaacccaaa gtacgaaaca ctcgattatt
    45541 cagtgaagca atctctgcca actgaaaggc attgatacta ggatggatca cagcaccgcc
    45601 gacactttta ttatatgctg tcgaaccagt aggcgtagaa atcgtcaatc catctcctct
    45661 aaaactttca aacagctcgt ttttgatgta tacatcggct accatcgtac gattggcccg
    45721 tttaatcgtt gattcattca gtgctaaaaa atgctggtcc ggtgtttcat ccaaataact
    45781 gatccgaaca tccagcaagg gataactaac actttgttcc cggttcgttc gtaagctgtc
    45841 tactagttct tccagctcgt aatcccgcca atccgtgtaa aaccctaaat gacctgtatg
    45901 cacgcccaag aaacgcacat cattcagaca atgactgtag tgatggaacg ccgacaataa
    45961 tgtgccgtct ccgccgactg agatgaccaa ttctggctca tgatcatctc gttgaatccc
    46021 tgcttgttct aaaagtgcgt tcaaacgttc tgtcacttct atagtctttg cttctttatt
    46081 atgaacgatc gctactttca tcccctgcct cctctctttc gctttctttc ttcaaaagcg
    46141 ttcgatagta gacatcatct gattttcctc ttccatggga gaataaccgc tgtgcttctt
    46201 ggatttcttc acggatactg gacatctctt catccaactg ataggcagct tctgccgcac
    46261 gctgaagtcg ttcactcatt tcttctggaa aaacgccttg atatttataa ttcagcgaat
    46321 gctcaatcgt tgcccagaaa ttcatcgcaa gagtccgaat ctggatttcc gccaaaattt
    46381 tcttctcacc gctgatcagc tgtacaggat actcgatcac cagatgataa gaacggtagc
    46441 cacttgcttt tttatttgtg atgtaatctc gttcttggag aactttcatg tcttttcttt
    46501 tacggatgat ctcaacaact tgatggatat cttctacaaa ctgacacatg atccttaacc
    46561 cggcaatatc agacatttct tcttctaaac gtcccattgg aatatgccgt aattgtgatt
    46621 ttgttacaat actatcgact ggcttgactc tcccagtgac aaattcaatc gggacatgtc
    46681 gattttgttc acggaactgt ttgcggatcc cccgcagttt cactttcaat tctgatacag
    46741 tttgttcata tggtgctaaa aatgcttccc aatctcgttc catagctgat acctctactt
    46801 cattaacatt cattgtttat tctaccatat atgcaaagtt ttttcgtact cttgatttat
    46861 gcattgacca aaaaaagaaa attaggcaaa atagacaaaa aggagtcgat caaatgacac
    46921 aaatgcttga aatcgaatat aaatctatgc tgacaaagca agagtacgag atattcatcg
    46981 ctcactatca attaacagat aaagatttcc gtgtacagac taatatttat ttcgataccg
    47041 ttgatgaaca gctgaagaaa caaaattgtg gcctacggat ccgtttcgtt ggtcatcaag
    47101 ctgaatatac attaaaaaca cctgctgaaa aagggcgtct cgaaacaacg gataccttta
    47161 taacagaaga agcagaagca tttatcttcg aaaaacgtct gcctcgttca ggcgcagttt
    47221 ttcaaaaact ttctgatttg aatatcgatc ctgcttcatt aatccaaacc ggaaagctta
    47281 caacaaaacg agctgaattc cctattgaag aaggactttt agctattgat gaaagctggg
    47341 gagacaatct acacgatttt gaactggaac tagaggtagg ggatgcatct gtgggaaaaa
    47401 ttgcttttat aaatttttta aaacgttttt ctttacctta tcgtcctgct aagaacaaga
    47461 tccaacgtat gcttgaagct aaaaattaga aacctttcat tttcacaagg cacttgagtt
    47521 cattttttta cttttttctg ttaagttata ggatgtgaaa gaatctagtc actagttgta
    47581 tagaatctat tttgggggag attcatatga tcgaaatcta tttatttgtt aatcctttag
    47641 gggaatactg tttcgaaatg gaacaacagt tattacaatt tatcgaagaa gagtacggga
    47701 caccatcaaa agaaaagatg caatttcgct ttcttccttt ggtcaatttg cagacaattg
    47761 gggatgtcat gcaacaaaaa ggaatttctc agaacaatct tgaggaacgt aatcacttat
    47821 tctcaatgac ttattctgca gcattggact gcaaagcggc gcagtttcaa ggaaagaaaa
    47881 aaggccgccg ttttttgatc aaattgcaag aagcagtcgg ctgtaatgac ataccatatt
    47941 ctcaagaact tgctgaatcc atatttgaag aaatcggcgg cgatttgaat atgtttaaag
    48001 aagatcgtca gtctgacttc gttaaagaag tattcttttc tgatcaaaaa attgctcggg
    48061 aaatgggcat cacaaaacac ccttctgcag tcgtttacaa ttatgcctgc gaccgtgatt
    48121 ttggtgtttt agtagaagta aaaggtacaa gtagcctttg gagaatcaca gagctgtgta
    48181 aaacaacaga taactcatat caagttttta atggtgaaca tttacataca aatgattctc
    48241 agttccattc aagaactagc catttacact tgctttctaa ttaaaaa
//
LOCUS       DASJSZ010000012        42778 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.012, whole genome shotgun sequence.
ACCESSION   DASJSZ010000012 DASJSZ010000000
VERSION     DASJSZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.012"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(122..499)
                     /locus_tag="U4B03_000652"
     CDS             complement(122..499)
                     /locus_tag="U4B03_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067526.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /locus_tag="U4B03_000653"
     CDS             755..1483
                     /locus_tag="U4B03_000653"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEN2067527.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="U4B03_000654"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="U4B03_000654"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEN2067528.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="U4B03_000655"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="U4B03_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEN2067529.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="U4B03_000656"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="U4B03_000656"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEN2067530.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /locus_tag="U4B03_000657"
     CDS             4618..6057
                     /locus_tag="U4B03_000657"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEN2067531.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6080..7132
                     /locus_tag="U4B03_000658"
     CDS             6080..7132
                     /locus_tag="U4B03_000658"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEN2067532.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="U4B03_000659"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="U4B03_000659"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEN2067533.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="U4B03_000660"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="U4B03_000660"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEN2067534.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="U4B03_000661"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="U4B03_000661"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEN2067535.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="U4B03_000662"
     CDS             10651..11565
                     /locus_tag="U4B03_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2067536.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="U4B03_000663"
     CDS             complement(11698..13263)
                     /locus_tag="U4B03_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2067537.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="U4B03_000664"
     CDS             complement(13256..14707)
                     /locus_tag="U4B03_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2067538.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="U4B03_000665"
     CDS             complement(14721..15737)
                     /locus_tag="U4B03_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2067539.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /locus_tag="U4B03_000666"
     CDS             15957..17045
                     /locus_tag="U4B03_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEN2067540.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /locus_tag="U4B03_000667"
     CDS             17099..18625
                     /locus_tag="U4B03_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEN2067541.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /locus_tag="U4B03_000668"
     CDS             18627..19832
                     /locus_tag="U4B03_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2067542.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="U4B03_000669"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="U4B03_000669"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEN2067543.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..22754
                     /locus_tag="U4B03_000670"
     CDS             21330..22754
                     /locus_tag="U4B03_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN2067544.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23215..24324
                     /gene="pdhA"
                     /locus_tag="U4B03_000671"
     CDS             23215..24324
                     /gene="pdhA"
                     /locus_tag="U4B03_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEN2067545.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24327..25304
                     /locus_tag="U4B03_000672"
     CDS             24327..25304
                     /locus_tag="U4B03_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEN2067546.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25329..26972
                     /locus_tag="U4B03_000673"
     CDS             25329..26972
                     /locus_tag="U4B03_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEN2067547.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26980..28386
                     /gene="lpdA"
                     /locus_tag="U4B03_000674"
     CDS             26980..28386
                     /gene="lpdA"
                     /locus_tag="U4B03_000674"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEN2067548.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28548..28823
                     /locus_tag="U4B03_000675"
     CDS             28548..28823
                     /locus_tag="U4B03_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEN2067549.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28829..29596
                     /locus_tag="U4B03_000676"
     CDS             28829..29596
                     /locus_tag="U4B03_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEN2067550.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29764..31593
                     /gene="typA"
                     /locus_tag="U4B03_000677"
     CDS             29764..31593
                     /gene="typA"
                     /locus_tag="U4B03_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEN2067551.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31854..33626
                     /gene="recQ"
                     /locus_tag="U4B03_000678"
     CDS             31854..33626
                     /gene="recQ"
                     /locus_tag="U4B03_000678"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEN2067552.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33740..34042
                     /locus_tag="U4B03_000679"
     CDS             33740..34042
                     /locus_tag="U4B03_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEN2067553.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34045..35208
                     /locus_tag="U4B03_000680"
     CDS             34045..35208
                     /locus_tag="U4B03_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN2067554.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35234..38662
                     /locus_tag="U4B03_000681"
     CDS             35234..38662
                     /locus_tag="U4B03_000681"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEN2067555.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38683..39807
                     /locus_tag="U4B03_000682"
     CDS             38683..39807
                     /locus_tag="U4B03_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067556.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39797..40234
                     /locus_tag="U4B03_000683"
     CDS             39797..40234
                     /locus_tag="U4B03_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2067557.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40218..40556
                     /locus_tag="U4B03_000684"
     CDS             40218..40556
                     /locus_tag="U4B03_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEN2067558.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40675..41235
                     /gene="rsmD"
                     /locus_tag="U4B03_000685"
     CDS             40675..41235
                     /gene="rsmD"
                     /locus_tag="U4B03_000685"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEN2067559.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41232..41723
                     /gene="coaD"
                     /locus_tag="U4B03_000686"
     CDS             41232..41723
                     /gene="coaD"
                     /locus_tag="U4B03_000686"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEN2067560.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41713..42756
                     /locus_tag="U4B03_000687"
     CDS             41713..42756
                     /locus_tag="U4B03_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEN2067561.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT        14111 a         7333 c         9555 g        11779 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    22801 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    22861 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    22921 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    22981 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    23041 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    23101 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    23161 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    23221 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    23281 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    23341 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    23401 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    23461 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    23521 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    23581 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    23641 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    23701 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    23761 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    23821 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    23881 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    23941 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    24001 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    24061 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    24121 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    24181 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    24241 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    24301 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    24361 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    24421 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    24481 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    24541 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    24601 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    24661 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    24721 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    24781 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    24841 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    24901 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    24961 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    25021 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    25081 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    25141 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    25201 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    25261 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    25321 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    25381 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    25441 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    25501 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    25561 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    25621 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    25681 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    25741 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    25801 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    25861 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    25921 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    25981 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    26041 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    26101 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    26161 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    26221 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    26281 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    26341 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    26401 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    26461 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    26521 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    26581 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    26641 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    26701 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    26761 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    26821 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    26881 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    26941 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    27001 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    27061 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    27121 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    27181 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    27241 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    27301 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    27361 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    27421 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    27481 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    27541 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    27601 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    27661 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    27721 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    27781 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    27841 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    27901 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    27961 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    28021 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    28081 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    28141 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    28201 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    28261 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    28321 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    28381 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    28441 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    28501 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    28561 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    28621 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    28681 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    28741 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    28801 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    28861 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    28921 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    28981 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    29041 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    29101 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    29161 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    29221 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    29281 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    29341 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    29401 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    29461 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    29521 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    29581 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    29641 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    29701 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    29761 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    29821 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    29881 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    29941 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    30001 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    30061 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    30121 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    30181 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    30241 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    30301 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    30361 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    30421 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    30481 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    30541 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    30601 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    30661 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    30721 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    30781 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    30841 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    30901 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    30961 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    31021 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    31081 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    31141 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    31201 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    31261 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    31321 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    31381 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    31441 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    31501 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    31561 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    31621 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    31681 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa
    31741 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    31801 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    31861 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    31921 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    31981 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    32041 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    32101 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    32161 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    32221 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    32281 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    32341 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    32401 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    32461 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    32521 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    32581 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    32641 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    32701 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    32761 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    32821 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    32881 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    32941 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    33001 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    33061 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    33121 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    33181 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    33241 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    33301 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    33361 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    33421 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    33481 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    33541 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    33601 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    33661 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    33721 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    33781 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    33841 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    33901 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    33961 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    34021 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    34081 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    34141 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    34201 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    34261 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    34321 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    34381 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    34441 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    34501 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    34561 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    34621 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    34681 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    34741 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    34801 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    34861 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    34921 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    34981 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    35041 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    35101 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    35161 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    35221 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    35281 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    35341 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    35401 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    35461 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    35521 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    35581 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    35641 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    35701 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    35761 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    35821 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    35881 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    35941 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    36001 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    36061 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    36121 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    36181 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    36241 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    36301 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    36361 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    36421 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    36481 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    36541 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    36601 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    36661 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    36721 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    36781 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    36841 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    36901 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    36961 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    37021 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    37081 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    37141 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    37201 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    37261 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    37321 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    37381 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    37441 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    37501 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    37561 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    37621 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    37681 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    37741 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    37801 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    37861 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    37921 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    37981 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    38041 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    38101 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    38161 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    38221 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    38281 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    38341 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    38401 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    38461 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    38521 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    38581 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    38641 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    38701 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    38761 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    38821 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    38881 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    38941 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    39001 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    39061 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    39121 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    39181 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    39241 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    39301 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    39361 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    39421 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    39481 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    39541 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    39601 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    39661 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    39721 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    39781 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    39841 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    39901 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    39961 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    40021 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    40081 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    40141 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    40201 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    40261 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    40321 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    40381 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    40441 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    40501 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    40561 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    40621 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    40681 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    40741 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    40801 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    40861 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    40921 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    40981 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    41041 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    41101 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    41161 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    41221 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    41281 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    41341 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    41401 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    41461 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    41521 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    41581 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    41641 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    41701 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    41761 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    41821 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    41881 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    41941 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    42001 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    42061 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    42121 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    42181 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    42241 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    42301 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    42361 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    42421 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    42481 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    42541 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    42601 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    42661 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    42721 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggt
//
LOCUS       DASJSZ010000013        42099 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.013, whole genome shotgun sequence.
ACCESSION   DASJSZ010000013 DASJSZ010000000
VERSION     DASJSZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.013"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            147..794
                     /locus_tag="U4B03_000688"
     CDS             147..794
                     /locus_tag="U4B03_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEN2067562.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            809..2335
                     /locus_tag="U4B03_000689"
     CDS             809..2335
                     /locus_tag="U4B03_000689"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEN2067563.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2384..5182)
                     /gene="ileS"
                     /locus_tag="U4B03_000690"
     CDS             complement(2384..5182)
                     /gene="ileS"
                     /locus_tag="U4B03_000690"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEN2067564.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5444..6151)
                     /locus_tag="U4B03_000691"
     CDS             complement(5444..6151)
                     /locus_tag="U4B03_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEN2067565.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6172..6954)
                     /locus_tag="U4B03_000692"
     CDS             complement(6172..6954)
                     /locus_tag="U4B03_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN2067566.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(7003..7290)
                     /locus_tag="U4B03_000693"
     CDS             complement(7003..7290)
                     /locus_tag="U4B03_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEN2067567.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7305..7904)
                     /locus_tag="U4B03_000694"
     CDS             complement(7305..7904)
                     /locus_tag="U4B03_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEN2067568.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7918..8595)
                     /locus_tag="U4B03_000695"
     CDS             complement(7918..8595)
                     /locus_tag="U4B03_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEN2067569.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8612..9853)
                     /gene="ftsZ"
                     /locus_tag="U4B03_000696"
     CDS             complement(8612..9853)
                     /gene="ftsZ"
                     /locus_tag="U4B03_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEN2067570.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9876..11201)
                     /gene="ftsA"
                     /locus_tag="U4B03_000697"
     CDS             complement(9876..11201)
                     /gene="ftsA"
                     /locus_tag="U4B03_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEN2067571.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11357..12577)
                     /locus_tag="U4B03_000698"
     CDS             complement(11357..12577)
                     /locus_tag="U4B03_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEN2067572.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12592..13680)
                     /gene="murG"
                     /locus_tag="U4B03_000699"
     CDS             complement(12592..13680)
                     /gene="murG"
                     /locus_tag="U4B03_000699"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEN2067573.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13706..15067)
                     /locus_tag="U4B03_000700"
     CDS             complement(13706..15067)
                     /locus_tag="U4B03_000700"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEN2067574.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15081..16043)
                     /locus_tag="U4B03_000701"
     CDS             complement(15081..16043)
                     /locus_tag="U4B03_000701"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEN2067575.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16069..18261)
                     /locus_tag="U4B03_000702"
     CDS             complement(16069..18261)
                     /locus_tag="U4B03_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN2067576.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18262..18663)
                     /gene="ftsL"
                     /locus_tag="U4B03_000703"
     CDS             complement(18262..18663)
                     /gene="ftsL"
                     /locus_tag="U4B03_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEN2067577.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18668..19627)
                     /gene="rsmH"
                     /locus_tag="U4B03_000704"
     CDS             complement(18668..19627)
                     /gene="rsmH"
                     /locus_tag="U4B03_000704"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEN2067578.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19648..20079)
                     /gene="mraZ"
                     /locus_tag="U4B03_000705"
     CDS             complement(19648..20079)
                     /gene="mraZ"
                     /locus_tag="U4B03_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEN2067579.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20249..20617)
                     /locus_tag="U4B03_000706"
     CDS             complement(20249..20617)
                     /locus_tag="U4B03_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEN2067580.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20788..21744
                     /locus_tag="U4B03_000707"
     CDS             20788..21744
                     /locus_tag="U4B03_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2067581.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21817..21933
                     /locus_tag="U4B03_000708"
     CDS             21817..21933
                     /locus_tag="U4B03_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEN2067582.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21988..23670)
                     /gene="recN"
                     /locus_tag="U4B03_000709"
     CDS             complement(21988..23670)
                     /gene="recN"
                     /locus_tag="U4B03_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEN2067583.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23688..24137)
                     /locus_tag="U4B03_000710"
     CDS             complement(23688..24137)
                     /locus_tag="U4B03_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN2067584.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24244..25062)
                     /locus_tag="U4B03_000711"
     CDS             complement(24244..25062)
                     /locus_tag="U4B03_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEN2067585.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25071..25961)
                     /locus_tag="U4B03_000712"
     CDS             complement(25071..25961)
                     /locus_tag="U4B03_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN2067586.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25963..26187)
                     /locus_tag="U4B03_000713"
     CDS             complement(25963..26187)
                     /locus_tag="U4B03_000713"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEN2067587.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26192..27529)
                     /locus_tag="U4B03_000714"
     CDS             complement(26192..27529)
                     /locus_tag="U4B03_000714"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEN2067588.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27530..28384)
                     /locus_tag="U4B03_000715"
     CDS             complement(27530..28384)
                     /locus_tag="U4B03_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEN2067589.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28481..28930)
                     /gene="nusB"
                     /locus_tag="U4B03_000716"
     CDS             complement(28481..28930)
                     /gene="nusB"
                     /locus_tag="U4B03_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEN2067590.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28923..29348)
                     /locus_tag="U4B03_000717"
     CDS             complement(28923..29348)
                     /locus_tag="U4B03_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2067591.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29594..30658)
                     /locus_tag="U4B03_000718"
     CDS             complement(29594..30658)
                     /locus_tag="U4B03_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN2067592.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30830..31075)
                     /locus_tag="U4B03_000719"
     CDS             complement(30830..31075)
                     /locus_tag="U4B03_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2067593.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31214..31588)
                     /locus_tag="U4B03_000720"
     CDS             complement(31214..31588)
                     /locus_tag="U4B03_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEN2067594.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31671..32585)
                     /locus_tag="U4B03_000721"
     CDS             complement(31671..32585)
                     /locus_tag="U4B03_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEN2067595.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32606..33409)
                     /locus_tag="U4B03_000722"
     CDS             complement(32606..33409)
                     /locus_tag="U4B03_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEN2067596.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33442..34431)
                     /gene="manX"
                     /locus_tag="U4B03_000723"
     CDS             complement(33442..34431)
                     /gene="manX"
                     /locus_tag="U4B03_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEN2067597.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34534..35025)
                     /locus_tag="U4B03_000724"
     CDS             complement(34534..35025)
                     /locus_tag="U4B03_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2067598.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35216..38023)
                     /locus_tag="U4B03_000725"
     CDS             complement(35216..38023)
                     /locus_tag="U4B03_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2067599.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38457..38750)
                     /gene="rpmA"
                     /locus_tag="U4B03_000726"
     CDS             complement(38457..38750)
                     /gene="rpmA"
                     /locus_tag="U4B03_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEN2067600.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38763..39107)
                     /locus_tag="U4B03_000727"
     CDS             complement(38763..39107)
                     /locus_tag="U4B03_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEN2067601.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39134..39442)
                     /gene="rplU"
                     /locus_tag="U4B03_000728"
     CDS             complement(39134..39442)
                     /gene="rplU"
                     /locus_tag="U4B03_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEN2067602.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(39462..39538)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            39724..40365
                     /locus_tag="U4B03_000729"
     CDS             39724..40365
                     /locus_tag="U4B03_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN2067603.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40427..42058)
                     /locus_tag="U4B03_000730"
     CDS             complement(40427..42058)
                     /locus_tag="U4B03_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEN2067604.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT        11862 a         9168 c         7155 g        13914 t
ORIGIN      
        1 aagaatgagt cttctctatc atctttcgtt ttttgttgta aaaacgtcta aaaatccttt
       61 gttttttatt tagaaaaata gtcaattaac gttttttttg ttacacttac taatgatggt
      121 tttttaccat gaaaggatga tcgaatatga caccaaatcg agaagattat ttgaaaatca
      181 ttttagaact aggtggagat acaacgaaag tcaacaataa acaaatcgta tctagtctag
      241 atgtctcagc tgcatctgtc agtgaaatga tttctaaact tgtgaaagaa gagcttgtag
      301 aacattcccc ttatcaaggt gtacaattga ctgcaagcgg actgcaaaaa gctagcagtc
      361 tgattcgcaa acatcgctta tgggaagttt ttttagttga acatttagac tatagctgga
      421 atgaagtcca tgatgatgct gaagtattgg aacatgtgac ttcggaacat cttgcagatc
      481 atttagaaaa ctatttgaat catcctgaac attgtcctca tggaggatcg attcctaaaa
      541 aagaggaagt tgtccatgaa gaacgccgac aaacattgga gtcctatcct gtcggtacaa
      601 aagtgcgtat cgcacgagtg cttgatgaaa aagaattgtt ggattactta gtcactattg
      661 acctgaatat caacgaagaa tatgagatcg ttgacatcgc agcttatgaa ggaccgatca
      721 ctatccaaaa tgatagaaag aaaatccccg tcagctttaa ggcggccagc acgatttttg
      781 tcgataaaat ataaaggagt gtatccacat gtctaatgaa aaaaatgtat tgtttacgat
      841 attcggcggt acaggtgacc tagcccagcg taaattgtac ccgtctttgt tccgccttta
      901 cagaaaagga aatttaggcg agcattttgc tgtaatcgga accgctcgcc gtccctggag
      961 cgatgagcac taccgtgaag ttgtcaaaga aacaatccgt gcattgaatc cttcagatga
     1021 agaagcaact acctttgcaa gccattttta ctatcaatcc catgatgtaa atgattcaac
     1081 gcattatcag acattaaaag aattgtctga ccgattagat ggacactatc aattaaacgg
     1141 taaccgtgtc tattatttgg ctatggcacc tcaatttttt ggaacgatcg tttctcattt
     1201 gaaatcacaa aatattatga caaaaaatgg cttcaatcgt ttgatcatag aaaaaccgtt
     1261 cggttctgat taccagtctg cttttgaatt gaaccagcaa atcagagaag tattcccaga
     1321 acaagatatt ttcaggattg atcattattt aggaaaagaa atgatccaaa acatctcagc
     1381 tatccgtttt gccaataata tttttgaggc tcaatggaac aatcgctata tcgacaatat
     1441 ccaaattact tttggcgaaa acctcggtgt agaagatcgc gggggctact acgatcatag
     1501 tggcgcactt aaagacatgg tgcaaaatca tattttacag gttgtagcat tacttgcaat
     1561 ggaaccgcca gtagcatttt ccgaaaaaga aatccgtaca gaaaagatca aagcattgaa
     1621 agctatccgc atttataacg aagaagaagt gttagaaaac ttcgttcgcg ggcaatatgc
     1681 tcaaggtgaa ttggatggtg tacaattcaa gggctatcgc gaagaagaca aagtggatgc
     1741 tcaatcagag actgaaacat tcgtcgcagg caaattcact atcgataact tccgttggtc
     1801 aggtgttcct ttctatgtac gtacaggcaa acgtttgact gaaaaaggga ctcggatcaa
     1861 catcgtattc aaacaagttc ctgttaatgt attcaaaaca tctgtcgatg aaccatgtga
     1921 cgatacgaca cttccaccta atgttctaac gatctatatt cagccaactg aaggattttc
     1981 tttatccttg aatgggaaag aagtagggca aggatttgaa acggaaccaa tcaaattgga
     2041 atttagaaat agtgcagaaa tggttgaaaa tagcccagaa gcttacgaaa aattactatt
     2101 agatgcgttg aatggtgatg gaacgaactt ctctcattgg gaagaagtcg ctcaatcttg
     2161 gcatatcgtg gatgtcattc gaaaagcttg ggaccaaaca caaccggatt ttcccaacta
     2221 caaagctgga tcaatgggac ctaaagcagc tttcgaacta cttgaaaaag acggattcga
     2281 atggatctgg caaccagata actggtatcg tgaacgtgga aaattaaacg attgataaca
     2341 aaagggggtg tgacaaagaa catgtcacac ccccttttcc cttttacttt tcttcgaatc
     2401 cttcagctac agcttcagga tagaagtctt caacgatttt tgcacaacga ccgcatagat
     2461 gaggaagttt ttcatctact cctacatctt tacgaacttg gcgacagcga tcgcaagttt
     2521 ccccgtctgc tttttccact gtaatagcaa catcatcaaa taccatagcg ttatctggca
     2581 cttgttcatc tgctgccata acttcaaagt agtctggtga aacgatcaat aattgtgcaa
     2641 tatctgcatc caaagctttt aacatttcac gaacttgttg ttttggataa acagttagtt
     2701 ttgcttccaa agatttacca atcaatttgc tgtttcttgc ttcttccaat gcttttaaga
     2761 cattatcacg gaagtccatg aaagcagtcc aggtatccat caattcttct tggtttgcaa
     2821 actcttcata accaggaaac tcgcttaact gaacgtactc ctcttcttct ttcaggaatg
     2881 accagatttc ttctgctgta tgcggaatga taggagtcaa taattttgtc aaagctacag
     2941 ctgtttgata gaaaacagtt tgcatgcaac gacgttgata atcatcttct gcttcaatat
     3001 aaactacatc tttagcaaaa tccaggtaga atgacgatag atcaaccgtc aagaagttca
     3061 taactgtacg gtaaatatgc atgaagttat atttgtcgta cccttcttct cggatttctt
     3121 tgattacttg gttcaatcga acggtcatat atttgtctac agaacgtaaa tcattgtacg
     3181 aaacagtatc ttttttagga tcgaaatctt ctgtattcgc taataagaaa cgcatcgtat
     3241 ttctgatttt acgatatact tctgccactt gacttaagat atccatagaa acacgaacat
     3301 ctgcttcata atctacacta gatacccata agcgaaggat atctgcacca aattgtttga
     3361 tcactttttc tggtgcaatg atgtttccta aagatttact catcttacgg ccttcaccat
     3421 ctaaagcaaa tccttgagaa ataactgatt tgtatggtgc tacaccattg atagccacac
     3481 tagtcgtgat acttgagttg aaccagccac gatattggtc tgatccttct aaatacatat
     3541 ctgctgggaa tgataattct ggtcgctgac gtaaaacagc ttcatgagaa gaaccagaat
     3601 caaaccagac atccatgata tctttttctt ttgtaaattc gccattagga gaacctgggt
     3661 gggtaaatcc ttcaggaaga agttctttcg cttctttttc aaaccagata attgatccat
     3721 gttcagcaaa taattttgcc acatgttcga ttgtttctgg tgtgatgatt gcttctccat
     3781 tttcagcata gaaaatcgga agtggtactc cccatgcacg ctgacgggaa attacccaat
     3841 cgccgcggtc acggatcata ttatacaaac gtgttttacc ccatgggata acccactcta
     3901 ctttttctac ttcatccaaa atattctgac ggaatttatc gatagatgca aaccattgtg
     3961 gagttgcccg atagatcaca ggctttttcg tccgccaatc gtgaggataa ctatgagtaa
     4021 agaaatctag cttcagcaaa gctccttttt cttttaatag atcagtgatc ataggatttg
     4081 ctttgtcata aaagatacct tcaaatccag gtgcttcttc tgtaaacaat ccttttgagt
     4141 ctacaggtga cactacagat agtttatatt tgctaccagc gatatagtca tcttccccat
     4201 gtcctggagc agtatggact aatccagtac cagcgtctaa agtaacatga tcgcctaaaa
     4261 tcacaagtga ttcacgttca tagaatggat gacttgcagt catatattcc atttcttgtc
     4321 cagcaaattc ttttagaact gttacatttt cccaaccgat ttctgattgg actgtttcta
     4381 gcaaatcttt cgctacgaca aacttacggc catcggcttc caccaataca tacgtaaatt
     4441 ttggatttac agcaatacct tggtttgctg gcaatgtcca tggtgttgtc gtccaaatga
     4501 caaaagctgt atcttcatca agaatatctt tgccgtcttt gacattgaaa gctacaaaaa
     4561 tagatggaga ttttacatct ttatattcaa tttcagcttc cgctaaagaa gactcacttg
     4621 acggagacca gtaaattggt ttcaatcctt tgtaaatata gcctttttca gccattttac
     4681 caaatacgcg tacttgtgca gcttcataag aaggatctaa tgtaacataa ggatgttccc
     4741 aatctcctgc aactcctagg cgtttgaaat ctgctcgttg cttatctact tgggaaagtg
     4801 catattcacg acatttttcc aagtattctg ccatggacat ttctttacgt ttcacgcctt
     4861 tattagccaa tacttgttca atcggcaatc catgtgtatc ccatcctgga acatatggtg
     4921 cacgatagcc agacattgat tttgaacgaa tgataatgtc tttactgatt ttattcaatg
     4981 aatgtcctaa atggatatta ccattggcat atggaggtcc atcatgaaga acaaagctag
     5041 gttttccttc gttcaactct tgtctttttt ggtagattcg attttcttcc cattcttttt
     5101 gccactcgcc ttctcggttt ggcaaattac cacgcatagg gaattttgtt tttcctaatt
     5161 gcaaggtttc tttcattttc attcaaccat acactccttt atttgttttc cggacacaaa
     5221 aaagcatgtt cagccgcaag ggacgaacat gccgtggtac cacccaaatt tagttgcatc
     5281 tatctagtgc aaactctctg tgaccaataa cgaggtcagc cgctgcttct tactctaatt
     5341 tcggaaaagc aggtctcaag tagaggatct ttcaccatgc agtgtcttac cgaacttcca
     5401 ctctcatcgg ctcgctttaa gaataacaaa gtgcttttct gttctactcg ttttcatttt
     5461 caccaggcac gatcgagata tcaatagcct gtgtatcaaa tgccgtcata ggtgcttcta
     5521 tgactcctgt gtctgagttt acatcagtat cattttcatt gtcaagctgt tttgctaaaa
     5581 cttctttgat cacttcatgg ctgtcagtca cgtagctgga gaatggttgt aaaatttcat
     5641 cccattccgg acttttcact tgttctaatt gagactcaag catcaagctg atacgttgat
     5701 ggaatactcg tgtttttttc ttaagatctt cagtttctgt cgcaagctgt cttgcttttt
     5761 ctgttgcatc tgtcaagatt tttcttgctt tttcttctgc atcagttgtt aattgatgtg
     5821 cacgtttttc agcgttcgca actaactcat ctgctttgtt ttgtgcagaa gtaacgatca
     5881 cttcggattc cttgcttgca cttgttttta ctttatcagc cgtatcttga gctacgatga
     5941 ttgattggtt caaagcatct tttaattcat tgaagtactc aagtttttct tcagaatgtt
     6001 tgactgcttt ttctaattct cgatttcttt gagtcaattc ctcatagtca cgaacaacta
     6061 aatctaagaa atcatctact tcgtcttgat tatatccccg catttttgtc gggaatgtct
     6121 tattttggat atctaatgga gttaaagcca tgagtaaacc acctttttat tttatttacg
     6181 taatacaccg aacagtaccc tgtacttttc ttttttcgtc ttcccctcaa gttcttggat
     6241 ctggactctg ccataacctc tgactgaaat aatatctaat agttcaagta caaaatcagg
     6301 tcttgtattt tctgtccagt tcacttttac tttaccagct tcgatcagct gcttggaccg
     6361 ctgtctggaa atattataaa tctcagagat cacactgtct agtcgcatgg aactaactgt
     6421 tcctctttct tgtgtccagc tatctttcgg cataatcatt tctgtgtatt tgattggttc
     6481 caagcgtaca gcaacctttc caatctttgt tacttgtgca gcaacaaaat gatccacctc
     6541 atttgcaata aatacttgcc agcgatcacc gtccgagatg atatcgccaa agtaagagcg
     6601 tttgatcccc gttcccacta aagttccaag gattttccca tgacttaggc tggcaaattt
     6661 gctaggataa tggatttcat acaatgctgt ttcaaaatct tcctctacag gttcgtaata
     6721 atcggggaaa atcaaacatc tttttctttc tgcctgttca tatccgccat aaaacatgaa
     6781 acgcaaatca ctgctttgtc gaatcagtgt ttctaaaata taggattgtc ttgggtcaag
     6841 aaattcggtc agatagggtg catattgtcc ttgaacctgt tcaatccaat cgcctaccga
     6901 atcgataaat gtacgttcat ctgcccgaaa gtgctggtat acatttgcat ccaaaatttt
     6961 cccctcctat ctattctgag aataaagatt ttgttgcttc aatcaataaa ctaaatggac
     7021 agttatccat ataacgatct gtgctaaacc ttgtacggct aagttcaaga tcaggattgc
     7081 ccctaaaatt gtgaaatcca agggtccaat cctcaaaggc agacgatcga acaaactgat
     7141 atacggttcg cagatcttag ctaataatcg tcctagccct gattgatacc cgcctggaaa
     7201 ccaagagagc aatgcatata ccatcaagat aatgctatag aaataggctg cccgttgaag
     7261 taaatgaagt aatagtgcaa tagtataaat cgtagttcct cctcttacag atcaaacaaa
     7321 ttcgcatctg caaaagattg agcggttgaa ctgtcaatct ccatcccagt cggtgtacaa
     7381 agaaaaatct catctccaac acgtttgata tctccatcta gtgcataaac tgttcctgtt
     7441 agaaaatcaa cgattcttct cgcttgtttt tcttctacta aatgaaaatt cactaaggtt
     7501 gcttctccag caatgattcg tttagcaatc gccatagctt cagaataagc tcttggttcc
     7561 attacagtga tttttccagc aagatttgct ttaggttgtg tagaggttct tgaagctgtt
     7621 ctttctttcg ctacagtttt tgtaggtgat tgatgtagtt caattacttt tttcttttca
     7681 gccggctgaa tactttcatg cgttacagga cgtgaagtcg ccgttttttg tttttcatga
     7741 gtattcgcag cagatacaga atgagttgat tgattctgtg ttgtattttt tttagttggc
     7801 tgtacagcct tttttgttgt ttcaggataa tcttggtatt cgtactcttc ctcatcagct
     7861 aatccgaaaa aattagcaat tgctcctctg tttaaaagcg gcatttttgt tcctccttta
     7921 ttcgtctttg aatagcgctg ttcctacacg gataaatgtt gcaccttctt caatcgcaat
     7981 ttgaaagtct tgactcatcc ccatacttaa ttcactacat ggtgcatggg cataaccttt
     8041 tgcttgaaca agatcacgca attctttcag cgtaccaaac actttatgga tttgctcatc
     8101 agaagcttct ttaggcgcca tagtcatcaa cccaacaacc ttgatttttg tgcaggcagc
     8161 taaagattcc acaaacggta aaagctcatc tggtgaaatt ccatgttttg attcctcacc
     8221 agaaacattt acttcaataa aacaggataa ttctttctct aaccgcttct ggatttcttc
     8281 tgctaatctt aaactgtcca acgcatgaaa gtaatcaatt tcatttacaa tcaattttac
     8341 ctttctgcgc tgtaaattac caattaaatg ccactttatt tcaggaaaat caaataaatc
     8401 ttgtttcttt tctaataatt tatctactcg gttttctgca caattcataa cgccagctct
     8461 aaccaattct tcagcttgag cggattcaac agattttgtt acagctacta atgtaacttc
     8521 tttcggatca cgatgaacaa gagcacacga atcctgaatt tcttgcttga ttttctgcaa
     8581 gttgtcagca atcatgtgct cttcctctcc tttatttttt acgacggaaa aacggtggcg
     8641 tatttaattc atcatcgcca tgagattttg tttcttcgcg gttgaatgta tcaaattctt
     8701 ttttttcgat attgtcaaaa ttggaatcat caactctagg acgaacattt tgttcacgtc
     8761 tgatatccca atcgccaaaa ctgttttctt cttctgaaga agttggttta gcttgatcca
     8821 tatctaatac agtcttttga gcaggtgatt gcatttgtgc ttgtctagct ggtcggctag
     8881 acttgcgttc cttttttgat tcatcgatac ctgttgcgat gaccgtcaca cggatttcat
     8941 cgcccatttc ttcattgata gatgtaccta gaatgatatt tacatcgcca gttgctgcat
     9001 ttgctacgat atctgaagca tcttgtgctt cgaacaatgt catatccaaa ccgcctgtga
     9061 tgttaagaag tacttgttca gctccatcaa tagatgtttc aagcaatgga gaggagattg
     9121 cttttttcgt tgcttctact acgcgatctt ctccactagc cacaccgatc cccatcaatg
     9181 ctgtaccttg gttttccatc actgttttca cgtcagcaaa gtcaaggtta acgtaacctg
     9241 gcgctgtgat caaatcagag ataccttgta caccttgtcg taaaacatta tctgcttcac
     9301 ggaaagcttc aagcatcggt gtctttttat ctacaacttc taataaacgg ttatttgaga
     9361 taattaataa tgtatccaca ttttctttca aacgagcgat accttctgca gcaaaacggc
     9421 cacgtttcgg tccttcaaaa gtgaatggac gtgtcacgac accaacagtc aatgcgccca
     9481 attctttcgc gatacctgca acgattggtg cagcacctgt accggttcct ccacccattc
     9541 ctgcagtgat gaaaatcata tctgcaccgt ctaaagcttc gcgtaaggat tgttcacttt
     9601 cttctgccgc tttttgtcca acttctggct gagaaccagc acctaaacca cgagtatatt
     9661 tggggcctaa ttggataact gtttcagctt ttgaattttt taatgcttgc acgtctgtgt
     9721 tggctgtaat gaattcaaca cctttaacgt tttcttcaat cattcggttt acagcatttc
     9781 caccgccacc accgactccg atgactttga ttactgcgcc gtcattgata ttattatcaa
     9841 ttgagaattc catatcttat tcctcccagg atgaattaat caaaaatatt tgtaaagaaa
     9901 cccttgattt tattttttac attttctccg gactcttttg gttccggctc atcgtagatt
     9961 gtttcttcag gtgcttcata gctctcatat gtgtttgttg tttcttgttc aactaccatt
    10021 tgacgagctg ctgaggtttc acccgtaaca gcaatttttg ccaattgata cacttcactc
    10081 aagtttgctg aataatccac gatacttatt acatttgtaa ataccgggtt tctcagcccc
    10141 atgtgatttg gaacatacaa ttttacattt acgccgaaga tttcttgtgc caagtccaca
    10201 acaccaggaa gactagcagc tccgcctgtc aatacgacgc cacctggcaa ctctaatgct
    10261 tcgatttggt ccaatgcatc ttttgctttg ttgaaaatct gttccattcg agctgaaata
    10321 atttctgaca agtagcgttc atcgactttc actggttctg actgaccaat cacatctact
    10381 ggaaattctt catctggtga tgtccgttct gggtatgcat ccccgtagtt tattttcaac
    10441 gcttctgcat tgttaaatga cgtatttaat acaatagaga tatctttagt gacaaattcg
    10501 ccaccttctt ggtcaagatt ggtaaacttc aattgcttat catgcataac agccgttgtt
    10561 gtttggccgc cacccatatc aatgacgatc gtaccaaaat ctttttctcc gtcagaaaga
    10621 attgattcag tcagcgctaa aggagtaatc accatttcgt tgacgattaa tccggcattt
    10681 tccacgcatt tacgtatgtt atggatgatc gttttaggtc ctgtaaacaa cagaccgtac
    10741 atttctaatc ttacgccgat cattccacga ggatctttga ttccctcgaa cccatcgaca
    10801 gtaaaatctt gtggcaatat tgacacgatt tgacgttcag gaggtattga acgaacaagg
    10861 gcagctgaag ccacatttct gacatcttca tcggtgattt cctttgaatc tccgttaact
    10921 gcaatcattc cctggcaatt ttctacttca agcatgtttg ctggcaaacc gacacttacg
    10981 cctttgatct ggatacctgc tttttcttct gcttgtcgta ctgctcgttg tatcgcttgg
    11041 accgttttat cgatatcaac gataatgcct cggttaatcc cttctgattt tgcgtttcct
    11101 actccaataa tattcatttg gctgtcgata tattcagcca cgacaacttt aacagatgtc
    11161 gttccaatat caaggcctac atacattcct gtttttgcca tgaatgggat tccctcctac
    11221 ttactattcc atatacaagc gaacaactag cgtccgtttt aaaatatact tattaataga
    11281 tatattcact gcttccaatt ctaccataaa ttactggttt ttagaaagta cattcttact
    11341 ttttaaagtt gttttatcaa ttttcattta aacttgacgg agttgtactt ggattatctt
    11401 ctatttgact tgtttcactt gaatcatttg aatcagatga tccgtttgtg ctagacgatc
    11461 caggtgtttg tgctttttgc gtttctgcct cttcacttga ttcgtttgtt tgatccgcat
    11521 tttcttcact ttcagttgtt tcttccgatt ggcccgattc ttccgtttct tgagtgctac
    11581 cgtaaggatg cgagaagata ccaacttcca tgtcgatcac acctttttca tccatatctt
    11641 tggctacttg cgaataatat ttcatttggt tcgcaagatt attgatattt acaataactt
    11701 gattgccgtc attcatattc aattggatca gatttttatt gcttgctctt ggcgtgtact
    11761 tgatttcaga aattgctttt tgtaattctg atgataactg attgtattgc tttgttaatt
    11821 ctttgatttt tgcagagtct ttgaactctt ccaagatagg gagatttttt gtaggatttg
    11881 ctactttttc atctaatact gttccatttt caatcactgg gtagtattga ttgttttttg
    11941 cgatcaaagc aatctcttta tattctgtta catccaaatc gaattcattc atccctttga
    12001 aatgtatatt tgctgtttcg attcttggct gtgctttttt aaggcgatcg atataagtag
    12061 atcgatgaaa atactgtccc caaacatttt ctcctaatga caattttgta tcagagatga
    12121 tttcttggct gttgactgtc ttattgcctg atactgcaac tgcttgtaat ttgctataag
    12181 gagaaacata atagatcaaa aaaatcagcg gtatacccaa tacagtgatg atcaaagcta
    12241 accgacgaaa tagtactttg tgccgttgac tttttaaatt gggcagccgg ttcaaaaaag
    12301 aaccgttata tggtccaccg tttttctttt gcttgatctc ttcaaaaaat tcgatttttt
    12361 tctcggactg gctttcttct tgctgttcct cttgaaccgt tttttcttct tcagaatctg
    12421 aaaggggtgt atctaatgtt atgtcttctt tttttggctc ttggtttttc atctcttgtt
    12481 ctgccagata ttttcggtta gccttttgcc aaggagtcaa attgttttca tcagaagacg
    12541 gatgagacaa tttttttggt tcttttttct tactcactaa gaccaccgcc tttatttttt
    12601 gattatttgt tttactaagt cataaagacg ttttgaagca tcagggattc cttgttcttt
    12661 agaagctcgg cacatttgct tttgcaattc ttcatcaccc atgatttcgt tgactgtctg
    12721 acttagtgat tcacctgtta gttcattatc tgcaatcatt tttgctgctc ctgcgtgaac
    12781 aagactcatt gcatttttag tctgatgatc attcgtaaca tatgggctgg gaaccaacac
    12841 tgccggtaac cccagagctg tgaattctgc gatcgaagtt gcaccagcac gccccaccag
    12901 cagatcactg gaagccatga cctctgccat atttttgata taaggctgga cactaatatt
    12961 ttgtatttca ggatttattt tatttaggat ctcttcataa tatctatctc ctgaagcata
    13021 caaaacttgg taagaagaag gaatatattc acttaaaaaa actgatactg cttgattgat
    13081 tttaagtgct ccttgactac cgccaaaaat caagactgtt ttcttttctg gatccaaatc
    13141 aaatgttttc aaaatatctg attttttagt atctgctact tcttgtgctc gaggattacc
    13201 tacaagaact gctttgtttt caggaaaaaa ggaagccgca tctttaaatg aaagagcgat
    13261 ccgatccaca tatcggctta aaaatttatt ggtaacacct ggtacactgt tttgttcatg
    13321 gataactgtt ggaataccca ttttggctgc agcgtacact accgctccgg aaacatatcc
    13381 tccagtaccg ataacgatat ctggttgaaa atcactgagg attttttttg cttgacggat
    13441 actcttaaga aacaatcgga ccgtcttgat gttatgcatg gaaatttttc gtttaaatcc
    13501 ttgaatctcc aaggtatgga aaggcattcc tgtatcaggg acgattttgt tttctagacc
    13561 tcgttttgct cccacataca tgaattcagc attcggttct tgagtcttta cataattgac
    13621 aaaggcaaga gctggataaa tatgtccccc tgttcctccg cctgtaacca aaattttcat
    13681 tttttatcgc tcgcttctac tttttctatt ttaatcggtt cacagcttcc atgaatctgt
    13741 ttcctctagt ttcaaaattt gggtattgat cccagctggc attcgctggt gataataaaa
    13801 ctacatcacc atctgtactg ttttctaatg ctaaaggaac tgcttgttct acagaatcgg
    13861 ctgttactat tgtctcgatg cctgcttttt ttccagcatc catcaacttt tcttttgttt
    13921 ggccaaatac gaccattgct ttgattcctt ttaatgaagg aattaattca tcaaatgtat
    13981 ttcctctgtc taatccgcca gccaataaca cgaccttaga cgtttcaaat ccacttagag
    14041 ccattttagt cgctaaaata ttggttgctt ttgaatcatt ataaaatttt ctgccttgga
    14101 tttctcctac atattgcgta cgatgtggta cgccatggaa aaaatgcaac gtttctcgta
    14161 tagcttcatt tgatatacca tacaattttg ctacagaaat agcagctagt gcattttcta
    14221 cattatgctt acctggtacg ccaagttcag atatttccat tattttttct tttttatagt
    14281 atattgagta atcgtcagca tacacaccgt cttccaacac ttcttttgtc gagaaaggca
    14341 gtacgcgtgc ttttgttctt tgcgctaatt cttgtagttc tgactgattc cagttaagga
    14401 tcagataatc tttttctgtc atgttttttt gaaggttcca tttagctttg acatattctt
    14461 ttcttgtacc atgataatca atatgtgctt catagatatt ggtaatcacc gctatatgag
    14521 ggcggaaatc tgtgattccc atgagttgga agctagacaa ttccatcaca attttatctt
    14581 ctgattttgc ttcttgcgct acaccacttg ctggataccc aatattaccc gctaaacgtg
    14641 caattccttg atctgctcct gcattcagta gtagtcctgt catcgttgtt gtcgttgttt
    14701 ttccgttcgt tcctgtaatc ccaataattg ggcattccgc tacttcgtaa gctagctcaa
    14761 cttccgtaat taccggaata cccatttctt gggcttttgc tactaaagga tgactgtagg
    14821 gaatccctgg atttttgacc atcaatgaaa attcttcatc caacaattcg attggatgac
    14881 tacctgttat tacttttatc cctaaagaaa gcagctcttg tgcttccgga ttttcgtcaa
    14941 aaggttttcc atcattaacg gtgacaagtg cgcccaattc atgtaataat ttggccgcac
    15001 ttactccgct ttttgcgagt cctaagacaa gcactttctt attttcatat gttgatattt
    15061 ttttcatttc ccgtcatctc ctaccaaacg atccataatg tgatcaacga agtcacaatg
    15121 ctgatcaacc aaaaaactgt atcaatcttc cactccgacc atccgcacat ttcaaagtga
    15181 tgatgaattg gagacatttt aaaaattcgt tttcctgtaa gcttaaagga agtaacttga
    15241 agcatcacac ttgctgtttc cataacatag atcaaaccaa tcaaaagcaa tgtccattct
    15301 tggtttagca ttatagaaat tgccgctaaa agtccgccca acgctaatga accgacatct
    15361 cccataaaaa tcttggcagg ttttctgttg tagacgaaga aacccagtaa acctcctaaa
    15421 acacttaagc aaatgattaa tacatcatac tgatgctgat gccaagcaat tagaccgtaa
    15481 gctgcaaaag agatacttcc taagccagca actaatccat cgatgccatc tgtcagattt
    15541 actgcatttg aaaatccaac aagccagaag agagcgaaaa taccataaat gattcctaaa
    15601 ggtagatcta gtccgaagaa attcaataca cctggataac cttcagaacg gtaaacaaga
    15661 taaaagatga ttccccctag aatctgtcca atcaatttct gcttcgagtt cagtcccatg
    15721 tttcttttct tgaatatttt tataaaatca tcaagaaagc cgattactcc gtataacgct
    15781 aatacaaaaa gcaagatgaa aagagtagga gttagctgtt tttgccaagc tccaacccaa
    15841 attcctgtca aaatagagcc gatcagaaac actaagccgc ccattgtagg agttccggct
    15901 ttgctattgt gccactttgg tccctcctca cgaatcgcct gaccttgctt tttcatttga
    15961 aaataaccaa taaatagcgg cattgcggca attgtcattg cacaactgct gacaatggga
    16021 atcaatgctt gtgtccattc catgctcttt tctctcctat ttctcttctt attcttctaa
    16081 agtaaaacta agtttgtctt cagtgatttt ttcatatgga gcaagttctt gtttgacaca
    16141 ataaccatct ccatcaaaac taacttcaat accgaggatt ttccccagct tcatgatatc
    16201 tgcttttgac catcctgtta cgtcaggcat cagcttatca ccaccagtat ataggatcag
    16261 tttttctcca gagatcaact gatcaccgtt ggcagtagat tgtttttgta cttttttacc
    16321 attaccgatc actacaggtt gcagtccact tttttgagca tcagctgctg caacgtccgc
    16381 ttcaagatta cgataatcgg ctacggatac tttttctgtt tttgtttctg tatctgaatc
    16441 ttcttcagtt tctttaaaat ccattgcccg tttcatcaat ggatttgcga ttttagccaa
    16501 tgccattcga tcatatgtct tagggtgttt cattgttaga tacaagacat agtctggatc
    16561 ttcagaagga accatctcca caatcgaata caggtaggct gtatctcctg tttgatatcc
    16621 ccctgtatca gaagcgattt gggctgttcc gttttttgcc gatacattgt atccaggtac
    16681 actatataca ccataagcac taccgtagtt ttcgctttcg acaacatctc tcatatattc
    16741 acggactttt tctgttgttt cttttgaaaa aggttgccct agaacttctg tttgagttgt
    16801 tcgctcttct ccagtttggg gatccaccac tttgctgata tatcgaggtt gaatcattgt
    16861 accgttattc gcaatagaag tgaaagcttt catcatttgg aaattcgtca cacctaccgc
    16921 ttgtccataa gcactcattg ctcggtcgac aatatttaat gtaggtaaag caccgtttac
    16981 ttcatcatct aatccagaat gagtactttg tccaaatccc agtttttgta aatagttata
    17041 ccatcgtccg cctaatcgct gttccagtat gaccatcccg acgttacttg accatgaaag
    17101 agcttgacgc atggtcagca cacctttttc accaaagtca tggtcattga tcgtagcatc
    17161 ttctacttgg atttttccag attggaaggt ttcattctcg ttgaattctc cttcttcgat
    17221 agcagctgca gtagtgaaaa ctttcatggt tgatcctggt tcataactat cttgtaccaa
    17281 gaaattacgc cagatagcat cttttccagt caatccttcc attgtttcag gattgaaagt
    17341 aggtcgttgt cccattgcca agatttctcc ggtttttgct ttcatcagga ctgctgtcaa
    17401 ttcttctggc tgatattctt catttacttg atccatcagt gtttctaaat aactttgtag
    17461 tctgctgtct aaagtcgtgt agatatcttg tccgtcgaca gctttttctt cttctgccac
    17521 tgtacctggc agaggatttt gaaaattatc tttttgataa ataatcttac catctttacc
    17581 gctcaagata tcattgtaag cagactccaa acctagttta ccaactaagc cattctcatc
    17641 tttgtctgga actgcgtatc caataaaatg agaagaaaag acgccatttg gatacatccg
    17701 agcctgatgg tcgacaaaat aaagaccagc gacaccttct tttttcatgt ccgcttcgat
    17761 gttttgcttg gtttcttggg agatattttt cccatagcta ccaaattcaa cttgatacag
    17821 ctcttttttt gcgccgtcct ctaaaatatt cagtgcatct tttttgtcta tagaaagatt
    17881 cttatgcagg atttctgcta ttttatcaaa attcttttct tcaacataca gttttttatc
    17941 cccagaggta tatgtttttg agagtatcgc tttaattgaa taagaggagg catcttctgc
    18001 gagcgccaca ccgttacgat cataaatcgt tcctcttttt gcttttacta cttcacttcc
    18061 ttggtacaac tgttttgttt tttcagctaa tgaggtacca gcaacatgac ctccaataac
    18121 gatatacgag aaacggacgg caaataaaaa gaacaatcca atactcgtag caaataaaat
    18181 gatacctact tttttgcgat tgttcatcgg atttagattt ttcttttcca taaaccgccg
    18241 aattttattt ttaagactca tttacttcac tttccttaaa ttgtcgtcat taatagacaa
    18301 tcctttttct tctgcgattt gtttaatccg ttcagtacga gaaagttcgt ttttttcttg
    18361 ttctaaactc gttttttctt gctttttatc attgatttct gtttgtattg acgtgatttc
    18421 tttttcagct ccgctgatat ttgtgcgcag cataactgtt aaaacacata aagcaacaat
    18481 agcaatcgcc acgcttgcca cagcaatttt ttccaaagca gaaatctgct ttaatcgttt
    18541 tgccggtgat tgtggaagaa tgatttcttg attcgaagaa gaatcaggca tttgttgttc
    18601 cggctgatcc accaattcag gtaaatcata ttcgtaatct tgcattttct ttaattctgc
    18661 catcattcta ctcctctttc attcttattt tttctgcaat acgtaacttt gcactgcgtg
    18721 atcgattgtt ttctgttagc tcttcctctg aaggaaggat tggtttacga gtaatgactt
    18781 tcagttctgg ttggaattcg tcaggtatga caggaagtcc cggtggtaga tcttgtaccg
    18841 tactatattc tttaaacata cttttgacaa ttctgtcttc caatgaatgg aatgtgatta
    18901 cgctgattct tccattgact ttcaataacc ggattgcctg ttctaaagag gcctcttcgg
    18961 caccgagttc gtcatttact gcaatccgca cagcttgaaa aattcttttt gcaggatgcc
    19021 cgccttttct tcttgcggga gctgggatga cttgtttaat gatttcgaca agctcaccag
    19081 ttgtttggat tggttttgtt tttcgtactc gttcgatttc acgagcaatt tgtttggaaa
    19141 atttctcctc gccatagcgg aagaaaatct ttactaattc atggtaggaa tattcattaa
    19201 ttacgtgata agcggataaa ggagctgact gatccatgcg catatccaac ggtgcatctt
    19261 gatgataact aaatccacgt tctgcttcat ctagctgagg agaggaaacc cctagatcat
    19321 acaaaatgcc gtctacctta tctacatgct cactcaagcg ttcttctaat tctctgaagt
    19381 tcgctttgat aaatgtgacc ataccttgtt cgacataaga agctaaacgc agcttggcgt
    19441 gatcgattgc tttttgatct tggtcaaacg catacagatg ccctttagaa cttagttggc
    19501 ttaataaata ttcactatgt ccggcaccgc cgagtgtaca gtctacataa acgccatccg
    19561 gtttgatgtt caatccatct accgtttctt tcaacatgac tgtatagtgt tgaaatgttt
    19621 cagtcattag tcagcctctt cctattctta tagtccgaaa tcaatcatac tttcagcaat
    19681 ttcgtcaaag ttctcttctg ctgctgcaga aaaatcttgc catcgtgtct catcccaaat
    19741 ttcaattcgg ttagatacac cgatgactac gcaacctttt gtcaaagctg cataatttct
    19801 taacgtgctg ggtatattga ttcgtccttg tttatcaagt tcgcattcag tggcagccga
    19861 atagaagaat cgcacaaaag tacgtgcgtc ttttttagcc aacggcattt cgttcagttt
    19921 agcttccaga ttttcccatt cgctcatcgg atagccgaaa agacagccat ccaatccgcg
    19981 tgtcagaaca aacttttcgc caagttcttc acgaagtttt gagggcacga ttaaacgtcc
    20041 ttttgcatca atattatgtt ggaattcacc catcaacatt gaaagccccc cattcctcca
    20101 ctaactaaaa ttagtctacc acaatccccc actttctacc accttttctg ggaaatccgg
    20161 gaacaacttt ttttattcat aaacattttt ttaaaaatca agctgcaaaa agccttttaa
    20221 aagacttttt acagcttttc tatttttttc ataggtaaga aagtatgttc acaatgatca
    20281 agacagcgta caagacgatt gtcaacagaa aaaccaaacg ccaagtcatc ttgaaataac
    20341 gcggataaat aatttcttca taataatatg cttggaaaac agcaatacaa attcccatta
    20401 gcaaaacaga aataaagaaa aacggtaaga tagaaattgt ccctgtattt ctagacaatg
    20461 catgcatacc gccccacaaa aaaggaatcg ccaaatcagg ggcttttatt ttaaaacgac
    20521 gatttaacga aaataatttt acaagaaatt ggcaaccaaa caaaacgatt accgggtaaa
    20581 tataccaaaa caagataagt ggtgtgaatg tgcccatgaa cgtaaactcc ttttttatcg
    20641 ttgcttcaag tatactgtat atactttaca caactgtttc attttattta aatttatgca
    20701 gttatattct atatcttaca aacacattcc atgtattcca attccctatc cgttataata
    20761 aaagaaacag gcaacaggag gaaaatcatg atcaaatatt ttacattaga taatgagaaa
    20821 ctgcgtaata ccggaaagga aactgaccaa acgatctggt attgtgtaga acgtcctaat
    20881 caagaagaga ttcatcagct gacacatcaa tttgaaattc cgtcagatta catcacttcc
    20941 atattggacg atgcagaaaa ttcccgtaca gaagaattca atcaatcaaa gttgacaaag
    21001 ccagcgttat tattgttaca atatcctttt gctaaaacaa gtcctagcgg ttattttcaa
    21061 gtggatacat acccgctctc tattattttg acgcccaaaa agaagcttat cacaattacg
    21121 aatcatgaac cactgttttt aaagaaagtc tttagccaga cctttccaaa aagtgatatt
    21181 tcgcctaatc tcaatatctt ctttcaattc atttggcagg tcacactctc ttacaacagt
    21241 tatttagcta cgcttcaatc tcaatgcgat actttggaag ccaaacttca agtatccact
    21301 gaaaacaaac aactttatca aatcatggat atccaaaaaa gcctcgttta tttcagagaa
    21361 gcgacttctg ctaatttaga tgcattgaaa gaactagcgc aaaataaact tttccaagaa
    21421 aatcatgctt tgaaaaatca tttacgtgat gtattggtgg aaacagagca ggcaatgact
    21481 acttctcgca ttcaattaaa actagtagaa caaatgaatc agactttttc agcaatcgta
    21541 tcgaataatc tgaataatat tatgaaaatt ttaacttctt taacgattat tttaactata
    21601 ccgacaatcg tcggcagtat ctatggaatg aacattaagt tacctattgc tgaaagggat
    21661 gatgccttta tatggctagt tctattaaca ttatttattt gtatcgtaac tacctattat
    21721 ttaaaaaaag ggaagttttt ataaaaaagc cctcttgtgg aaatggaaaa aagattgtac
    21781 acttgaatca actaattaat gacgaggttg aaaacaatgc gtgaaaaaaa acaaacaagt
    21841 acttttgcta aagtaacaaa atttgtcatt tggacaatgc tgattttaac gattggatct
    21901 gttgtattga ctgctgtaat gagtgtcatg taaaaataaa atatagctat cagctgctga
    21961 aaaggacctg aaccactgcg aggtggctca ggtccttttt gcgagtttca atagttcttt
    22021 tgcatgttcg agtgtgagtt ttgtaatctc acttccagcc agcatacgtg caatttcttc
    22081 ttctctttct tttgttttca actctgaaac agaagtttgc gtacgtccat caatcactgc
    22141 tttcacaata taatattgat agtcggcgac tgcagcaact tgaggcagat gagtgatgca
    22201 caaaacttga gagtatttcg atatttgaga aattttatct gcgatggcct gtgcaactct
    22261 tccgctcaca cctgtatcca cttcatcaaa aataatactt gtcacacctt gctcagaaga
    22321 aaaaatagtt ttcaaagcaa gcaacatccg agacagttcg ccaccagaag cgactttcac
    22381 caaaggcttc agtggctctc ctggattggt cgtaatgtaa aattcgattt ggtcaaatcc
    22441 atcatgattc aattgtctat tttcttcttt taagaaacgt acctcaaact cagtgttttc
    22501 catatacaga cttttcaatt catgcagaat ggatttttcc aattcttttg cgattttttt
    22561 acgcgcttca tgaagatctg cggcttgttg ctgaagtaga agctcttttt gctctagttc
    22621 ctctttcatt ttgtccaatt gaccttctga aaaatcagaa gaagctagct cttcggtgat
    22681 ttcgtcatag taagccaata ttgactcaat ggattcgccg tatttacgtt tcaactgtcg
    22741 gattaattct aaacgttgcg taacttcttc taaacgactc tcatctaact ccaataaatc
    22801 aatctgacga ctcatatcgc cgattgcatc ttgcagcaaa tagtaagcac tttggatatt
    22861 ttcataaatt gcttcatact ccacatctag atgggcgatc ccttgcagtt ctgaaacggc
    22921 caagccaaca ccatctacac tgttttgttc attattcgtc agtgctccgt aacctgcagc
    22981 taatccatca gcaatttttt gataattgct aagtttttct ctttcttctt ttaatttctc
    23041 ttcttctccg acttgcaaat cagcagcagc tatttcttct tgctggaaat ggagcatatc
    23101 tattctctga acataggatt tttcattttg ctgcactttt cgtacccttt tttccagctc
    23161 acgataagag atatattctt gctgataact ctttttcttc tgttggaaag ctacatctcc
    23221 aaaacgatcc aataaagcaa gatgtgattc aggctggagc agttcctgat gttcgttttg
    23281 tccttggata tctactaaat aactgccgat tcttctcaga ttagctaatg tgataatatg
    23341 accattcact cgacaaacat tttttccagt taaggacata tcccgtcgaa caatcagatt
    23401 gtcctcatct gtttcgatcc ctaattcaac catcagttca gagaatcctt cttgttttgg
    23461 tagttcaaac aacccttcaa gcacacattt ttctgctcct tgacgtatat aatcgcttga
    23521 acctcttcct cctgctaaca gacctaaagc atcgataata atggatttac ccgcaccagt
    23581 ctctccggtc agcgcggtca ttccttcgtg aaaagaaagc cgcaattccg gaataatcgc
    23641 aaagttagta atactgattt cttgtagcat cacattcgct cctcaactta aaggtaatgc
    23701 aggaagtctt tcttcaataa aactgcctgg tcttcagttc gtgtaatcat caaaacatta
    23761 tcgtcatcat tgataatcga aaataattct ttcttgtaac gtttatcaat caaatttgct
    23821 gctgccgaag catttcccgg caatgttttc aaaataacaa atttttccat ttggtcaact
    23881 gccacgaaag cgtcttttaa tagtttttcc aatttagtat ttacatcttc gtttgtttca
    23941 gctggtatgc tataacggta gccgccatct gaagccggta ccttgatcag tttcagttcc
    24001 tttatatctc tggaaatcgt agcttgagta acagatattc ctttgctttt caggatttca
    24061 acaaattctt cctgctttcg gatatcttgc tcactcaaca atcgagtaat caaacgatgc
    24121 cgatcttttt ttctcattac gtcttcgctc cttagcatat tcttcttaat caatgatagc
    24181 ttatttcctt ggtataataa agaagaatcc gcatacttct cacttgattt tcacagttac
    24241 aatttattta tggtcaaact gttcgtgtgc atttgaaacc acttctgcca tattaatagc
    24301 tgaattgatt gttccgcttt cgggtacttt cctcaagtga gccaagaact cgatatttcc
    24361 ttctcccccc gtgatcggag aataacttaa ttccagcaca tcatatccac cttgtgccgc
    24421 aaatgacaaa atgtcttcta aaactttttg atgggtctga gggtcacgaa caattccgtt
    24481 tttccctaca gcttctctgc ctgcttcgaa ttggggtttg attaaagcaa tcacttctcc
    24541 ctcttctttt aaaatagcat gtaatggagg cagcatcagt tttagtgaaa taaaagaaac
    24601 atcgataaca gcaacttctg gaattcctaa ggtaaagtct tcgggttttg cataacgaaa
    24661 attcactctt tccattacga cgactctagg atcttgtctt attttccaag ctagctgatt
    24721 gtagccaacg tctaaagcat aactcatttt tgcgccattt tgcaaagctg catcagtaaa
    24781 tccgccagtg gatgcaccaa tatctaacaa tgtttttcct tcaacagata gatcaaatgc
    24841 tttgagtgct ttttctaatt ttaatccccc tcgagaaaca taaggaagtt ttttaccttt
    24901 gactcttaat tcactactca ctggaatttt ttctcccggt ttatcaaacc gctcgttttt
    24961 ttcgttgtaa atcaaaccag ccatcactga acgtttggct tgttctcttg tttcaaataa
    25021 tccttgtttg accgctaata catctacgcg ttctttttcc attctttccc tcatttctcc
    25081 acatcaaatt gcttgatcat ctggcgcatc agcccgccat caaaagaaaa aacgtcctct
    25141 tctaatttgt ctatgatctt gttggcggat aacagctcaa tctccaacgc ctcacgtgtc
    25201 ttttctaggc ctaataaacg cgggtaagta tttttttcca tacgttcatc tttaccagct
    25261 gttttcccta atgttttcgt tgtaccaatc acatcgagta gatcatctct tatctgaaaa
    25321 gctaaaccta aatgttcggc taatcgttga agtagcaata agatttcttc aggttgcttc
    25381 gctaatatgc caccagccag caaggcataa cgaatcaaat gccctgtctt tctttcatga
    25441 ataaatgcta attcttctaa agaaagcttt ttcgattcgc cctcgatatc tgcagcttgc
    25501 ccagcaacca taccttgtga tcccgcacaa acagctaatt gctgaagcag cagaagctta
    25561 ggagaattac ctaagtgtgc catactgatt agctggaatg ctcctgtaag caaaccatct
    25621 ccggccaata tagctaatgc ttcgccgaat actttatgat tggtaggttt tcctctacga
    25681 agatcatcat tgtccatcgc aggcaaatca tcgtggatca aggaatacgt atgcaccatt
    25741 tctaaagcag ctgctacttg atagacggga acatcaatag gctcatcaaa ggaagcaacg
    25801 gccgttaaaa gtaataaggg acgtattctt ttcccgcctg ctttcactga ataactcatc
    25861 gcatcataga gtcctctatc cgttgtatgc tcatctaaaa aagacaagat ttctttttct
    25921 acagcaggta gatgaatagc tgaaaagtca gttaatctca tctcagcttt cctcgtttcc
    25981 gtcaaaatca acttcttcat ttgattctgt catcattttc gtcaaggtct cttctgcttt
    26041 agctaatgta tcttggcaat gtttgcttaa ttccattcct cgtttaaacg aatctagagc
    26101 tgattctaat ggaacgtctc cttgttctaa ctgcatgaca attttttcta attcttgcag
    26161 tgactcttca aaagttggat tttccatatt tttattctcc tttgtctatc tcttgtactt
    26221 gtcctttaac gattccatct tgataatgga tcgtcagcaa atccccttga tgaatctcgg
    26281 tgacactttt tactacttga tcatttaacg tcgtatagct gtagcctctt cccatgattt
    26341 tcaatggact caacaaatca agagattgaa cagcttgagc aaaacgctgc tgctgttgtt
    26401 tcatatattg ttggattttc tcttctaagc gtttttctag ataagcagtt tcttggcgag
    26461 ctgctttaac tcgataaagc ggagccactt gttccaattg gtggcttaat gcgatcgttt
    26521 gtttttcttt cgcatgataa atttgctgtg tagcttgata taatcgctgt ttcaactggt
    26581 ctaatttgat ggtctgacct tcatacaatc tttcaggctg ccgaaataca taggaacgtc
    26641 gagcacgctc gaaccgttct tgtttgcgct ggatctggcg tacaaacgcc tgctgcaatc
    26701 tagcttgccg ttcttgaatg cgcatcaact cttctgacaa aacaggaaca gctaattctg
    26761 cagctgctgt tggagtggca gcccgaacat ctgctactaa atcagcgatc gtggtatctg
    26821 tttcatgtcc tacagatgaa atcaccggcg tattagcttg ataaatcgcc cgagcaaccc
    26881 gctcttcatt aaacggccat aaatcctcaa tcgaaccacc cccacgaccg atgatcattg
    26941 tatcgaaatt gcccatctct tctactctct ggatattcct gacaacattg tccgctgctt
    27001 ggtttccttg tacaactgtt gggaacaaaa ccaattggac aattggatat cttcttttca
    27061 ccgtcgtgat gatatcgcgg ataaccgctc cgcttggact ggtaaggacc gcgattcgct
    27121 ttgggaaacg tggcagcatt tttttaggcc cttgaaaaag gccttccttc atcagctttt
    27181 cttttcgttc ttccaattct tgatataaag cacctattcc atcaggctcc atgtgctcta
    27241 catagatttg ataagaacct cccgcttcat ataatgaaat acgcccaacg acaaggactt
    27301 tcattccttc ttttggctgg aatcgtaatt tttggaatgc tcctttgaac ataatagcag
    27361 atatttttgc atggtcatct tttaagctga aatattgatg ggcattggca cgtaaacgga
    27421 aattcgatat ttcgcctgtt aaatatacgc gttctaaata aggatcggca tcaaattttc
    27481 ttttcaaata cttcgttaat gtagtgacag ttaaatattc ttgtgccatc tattcacctg
    27541 ctccaaaagc ttcggcagat tttacggttt gatacatcag catcgtgata gtcattggcc
    27601 ctacaccctt tggcacagga gtgatccagc ctgcaatagg ctccacttca tcaaatttca
    27661 catcaccgat cagcttaccg ttagcatcac gattcatccc tacatctatc actacagcac
    27721 ctggttttac gaattctttt gtgacaaaat gtccacgtcc tattgctacg actaaaatat
    27781 ctgcttcttg tgccagttca gccagatttt cggttttaga atgagcaaca gtaacagttg
    27841 catctgccat caagagtagc tgagccatcg gtttaccgac gatattgctt cgtccgataa
    27901 ctactgctcg tttacccgcc ggatcgatag aataagcttc aaacattttc atgatgccgt
    27961 atggagtaca aggaatcttg tccggtttac ccgcaaggag tctgcctaaa ttcatcggat
    28021 gaaaaccgtc tacgtctttt tcagggcgga tagctaacag gatcttttct tcatctatat
    28081 gctttggcaa tggtaattgg accaatatac catgaaaact aggatcttga ttgtattttt
    28141 caatttctgc aagtagttcg tcttcagaaa tcgtctcagg ataacgctct accaatgaat
    28201 ggattccgat ttcttttgct acacgttctt tatttcttac atagatttgg ctggctggat
    28261 tttcccctac caataaaacg actaatccgg gatggatacc attatctttc aatttttgga
    28321 ttttttcagc aatctctgcc tgcatttttt cagctaattc ttttccgtta atcaattctg
    28381 ccatctaacc actcccagat attttttgtg ttttcattct accatagaat tttcccgatt
    28441 tataacaggt ttatgatgat tcatagaaaa aagctaggac ttacgcccca gcttctaatt
    28501 ctttcaatac attggataaa acaccgttga cgaatttacg tgaacggtca tcactaaatg
    28561 tttttgccaa ctcgattgct tcatttaaag caacagccgc tggtacatct gtcacataag
    28621 tcatttcaaa aatcgcgata cgcaagatca ccaaatccat ttttgagatc cgattgatgt
    28681 gccaattgtt ttttaaatgt ttttcaatca ctttatccaa ttcatctttt tttgcacaga
    28741 caccgccaac tagcgtatca agataaacag gaacaaactc tgattcatct tcgttgatca
    28801 tgtcgcggtg gtctaattcg attgcgttga aaatcgcatc ttcttttgtt aggtctgcat
    28861 tgaagtctaa tggaaataaa gcctgcaatg ccatttcccg aatctcgtgt cttgataatt
    28921 ctttattcat tctcttcctc ttcgtcttca aaaagttctt cgattgttgg ttgttctaat
    28981 ttttcaggaa caacagctac tacatggata ttgacttcag ataaatcgat atccgtcatg
    29041 aaaaggactt gctggctgac ccgttcctgc atttccattg ctactttagg aactgaaaca
    29101 ccatattcta gataacagta aagatctact ttcaagccat cttcctcgtt tgctaagtaa
    29161 acaccttttc cgtgagctgc acgtcctaat aattcagtga cattgctggc aaaagttccg
    29221 cgcattccgt atacgccttc tactttagaa gctgcaatcc cgataattac ttcgatgact
    29281 tcaggagcga tcacgatctc tcctaaatct tggtttgcgt ttaaaatcaa atttttttca
    29341 tctgccattt atttattcct ccctttgcct aagcacgaga tacatccgaa ttctctatga
    29401 tgcggcatag tgatcacgtg gtaagaaaac tgccgccgat cagctgcaaa ctgctatgaa
    29461 ttggagaaaa cacccaactt tattatctct tacagcttaa aagttcttct atagtgtatc
    29521 attaaatcga gtgattttct ataacaaagt ctgtgaacac tgatttttac gcttacttct
    29581 tctattgatg aaactataag atgatcaatt cttttggtga acgagtcaac acacgatttc
    29641 catcttctgt gatcaacaag tcatcctcaa tacgtactcc accgattcct ggtaagtaga
    29701 tccctggttc atcagtgatc acatttcctg gtacaaattg cttttctgca cgagaagaaa
    29761 cgtttggtcc ttcgtggatc tctaaaccga tcccatgtcc tgtagaatgt ccaaatgctt
    29821 ccccgtaacc atgttttgaa atataatctc tagcaactgc atctaattgg acaccagtca
    29881 ttcctggttt tgcagcatca ataactgcta attgagcatc taagactatt tgataaattt
    29941 cttttaattt ttccccagga tcaccaatag cgaatgtacg tgtcatatcg gaaacgtacc
    30001 cttcgtaata gcaaccaaag tccaatgtga tcaagtcacc ttgttcgatg atttttttgc
    30061 ttgctacacc atgcggcata gccgaacgca atccgctagc aacgatcgta tcaaaagaaa
    30121 cacctgaagc gccaagagaa cgcatataaa agtctagttg atttgcgact tcaatctcag
    30181 tcatgcccgg ttggatcatt ttcaggatgt gatcatatgc catatccgca atatgacatg
    30241 ctttttcgat aatagcgatt tcttcttcat ctttcacttc tctgagttct tcgatcatct
    30301 gtgaaacagg tactaattcc gcatcgatga tttcttctaa aaccgaatat tctaaaaatg
    30361 agactgttgt ttcctcaaaa gctaatgagt gcagttcttc tttgcgtact aagtttgcta
    30421 cctcatcaaa aataggtccg acatttttaa taatttcaaa cccttgagcc tgcgctgccg
    30481 cctgctcggt ataacgaaaa tcagtaataa aaaatgcttt ttccaaagta ataacagcta
    30541 atcctgtcgt acctgtaaag ttcgttaagt aacgaagatt gtacggactg gtaaccaaaa
    30601 atgaatccaa attttcttct tgcatttttt tgcgcaattt ttctacacgt agcatcataa
    30661 tgaaactccc tctttctttg cacgcttttt tccattatag caaacttttc ttcgtacacg
    30721 acagttaaat aaaaatttcc ggaaaacttc tttttatttt aaataaataa agccaaaaag
    30781 gagtcacagt ctcgctgcaa ctccttttag tttactattt tgtaaaaact tatgatcgtt
    30841 ttccaaagaa aaatgagaca actgctacca aaatcactgc gcctaaaata gatggaataa
    30901 gtgccatacc tgcagctttt ggtccccatt cgccgaaaat cgcttgtcca atacttgaac
    30961 cgactagtcc agcaatgata ttcgttacac agcccatcga atttccttta ctggtgatcg
    31021 cacctgcaat tgctccgatc acggctccaa caatcaatgt ccataaccaa ctcataatat
    31081 aacctccttc tttgctaatc aattttagca tatgggattt aaaggggaca aataattggt
    31141 tttgctattt catattttct tctgcaaagt aaaaaaataa agaaaacctc ctcttagaag
    31201 ttttctttat tttttacttg cgtttaaaca tactacgaat accacgtttc actacatcaa
    31261 agaaactcaa tgattgtacc attctatctt catctacata ttctctgatc gcacgaagca
    31321 cttttttagg ttctttggaa gaaaatgtgt atgtaccatt ttttttcgtt tgaatggcat
    31381 atcgaggaat ccattttccc tttagcatga ctgatgctag tacataatcc acttcttccc
    31441 atggaatttg gatatatttg cgtctatctc gcgcatggta aaactcaaaa ccattattac
    31501 ctatcatgat ttgcccgtaa tctgtcagac ccgtaaacga agtagcatca attaccaagt
    31561 ctacttttgt attgattgat tgaaccattt tctccctcca gatatttgtt tatgaacagt
    31621 atactatata aaaaaagctc gggacaacgc ccgagctata tttttatgga ttataaaagt
    31681 ccgatgacgt gtccaacaac accgactacg aataaaccaa ggatgataac gattggagaa
    31741 acctttttct taagcaacca catacataac aatgttaatc ctaatgcagc taaaccagga
    31801 atcaattggt ctaagttgtc ttgtaatgtt gttacctttt caggagaaag ggctaagcca
    31861 ttattaactt gttcaaatgc actcttgatt ccttcaccac ctgaaggcaa tttatcccat
    31921 tcgatgtaag cacctttatc taattttaca gttgatacaa ctggtaagaa tttgattgat
    31981 acccatcttt gtacaagggc agctaatacg aacatcccta aaattgaagc accttttgtt
    32041 acgtcttgta acaatccgcc agaaaggtca tcagtaattt tagaaccagc tttgtagcca
    32101 aattcttgtg tataccacat gaatgcccaa cgaataatgt tccaacctaa gaagaagatg
    32161 attggtccaa ggatatttcc tcccatagct aatgaagcac ctagtgcacc tagcattgga
    32221 cgtactgtaa accagaacac tgggtcacca acaccagcta aaggacccat catacctact
    32281 ttaacccctt gaatcgctac atcatcaaca ggcgcgccat tcgcacgttc ttcttccaga
    32341 gccaatgtaa cccctaaaat tggagaagca atgtatgggt gtgtattaaa gaattccaag
    32401 tgacgtttta atgctgcaga acgttcttct tttgttttgt ataatttttt gattgctgga
    32461 atcatagaaa atgcccagcc accgttttgc atacgttcgt agttccatga accttgaatg
    32521 aaggtagagc gccacgcaac agctaaacga tctttttttg ataatttgat tttttcttct
    32581 gccattttat tttcctcctt caagattaat agtcattcaa aatgtcgcct aaagggtcac
    32641 cagagttgct tccgcctcca ttatttgatg aaccacccat tttagaaagg ttcaagtaaa
    32701 taagagctaa tgcaacacct aaagcaccta gagcaatcaa tgttaattga gaaattgcag
    32761 ctacaacgaa accaataacg aagaatggcc atacttcttt tgtagccatc atgttgatta
    32821 ccaaagcgta acctacagct acgaccattc ccccaccgat agccatacca tctgttaacc
    32881 atgctggcat tgattcaagg aatgattgca cagtttgtgc tgggataaat agcaacgctg
    32941 ctgctgggac cgcgatacga accccttgta aacatacagc aatgatgtgc cacatttcaa
    33001 ccttacggat gtttccttct tcagcagccg catccatcaa atgaacgatt ggaacagcga
    33061 ttgtacgtac gatcattgtt aagaacaacc ctgcaacagc caaaggaacg gcaatcgcga
    33121 tcgcagaagg aacaccttta acaccttgtc cacctaaaac taaaataatt gctgatgcta
    33181 ctgatgctaa tgcagcatct ggtgctacag cggcaccgat atttgcccaa cctaaagcga
    33241 tcatctgaag cgtaccgcca agtacgatac cagcttcgag gttaccggta actaaaccga
    33301 tcaatgtaca tgccactagt ggttgatgaa attgaaattc atcaagaata ccttccatac
    33361 cagctagaaa ggcaactaat acgactaaaa ttattgaaat aatagacatg ataagcctcc
    33421 tataaatttt aatattattt attacgcttt tgctaattca gcttttgctt ttttcaaaat
    33481 atcatccatg ttatcgcgtg aatcattagg tactttacgt acatcgaatt tcacaccttt
    33541 ttgttcaagc ttctcaaacg cttcaacgtc ttcttgtccc atagacaata ctttacttac
    33601 aacgacttta ccgaccgaat gcgccattga cccaacattt aattctttga tgtctacgcc
    33661 accttcaaca gctgtcaata cgtcttcagg attttcaaat aaaagcaatg ctttggtatt
    33721 tccaaaacga gggtcttttg ctacttcgat cattttgcta ataggaataa catttgcttt
    33781 tacacctgga ggagcagctt gttcgattag tctcttacgc agatcatcct ttgatactgc
    33841 atctgaaaca acgataatac ggtttggttg cactgctttt gtccaagctg tcgcaacttg
    33901 tccatgtaac agacgtgagt caacacgtgc taatacatat ttgatttttc cgtctccgac
    33961 cacagttcct tctggaagtg ctcctttagg ttgtgcgtct tcaactgctg ctttcggtgc
    34021 ttctgccggt tctaattctt ccggttttac ttttacaccg tcttttgctg tttcaatgat
    34081 gtgtgctgca atttcatgag cagattccat tgaaaagcgt gatgcgtagg cttcaatcaa
    34141 cattggtaaa ttcaaaccgc tgacgattgc ccatttatct ttgtgctctt cgaataatgt
    34201 gtttgattga ttgaacggtg ttccgcccca taaatcaact aagaataaca cttcgtcttg
    34261 attatcaaaa gaagcaaccg cttcttccaa tttggctctc aagtcatctg gaccttcact
    34321 tggtttcaaa atcacggctt tgacgttttc ttgttcgcca aagatcattg aaccggattg
    34381 taagattcct tcagcaaatt gtccatgact tgctaggata atccctacca tatttatacc
    34441 tcctatttac ttttgttgta acaaattcaa aaatattaat aattgctaac cattacaaca
    34501 gaattatttg accttttcca acaaccattc ttgctacacc tttttgattt tcgctagtag
    34561 ttccatcaaa aggattttcc tgtcagcagg tacttttcga atctctaact ccacttcttg
    34621 actgttcaag taacgaaagg aatcaatatc ttgttgattt actgaaacaa aattggtaat
    34681 cattgatttg ccttcagtaa aactcatacc gccgatattc aacgacttca actgtacacc
    34741 gcagcggaca accgcttcaa catcttgggg attggtgaat aaaagcatca cttttgctcg
    34801 tgataataat ccactatgat agatttgaat cattttctgc acggttatta catttgctgt
    34861 gatccccggc ggtgcagctt cttttagtag aaactttctc aatttatctt gagcgactac
    34921 atcgctgatc acaatgatac gttcaacacc tgtttgcttc gaccaaactg ttgcgacttg
    34981 cccgtggatt aaacgatcat ctatacgtgc caatcgaata tccatctgca aaactccttc
    35041 ttcctcacta cttcacagaa acccctatca tttctgtgtg aacaaccacc tcctagcaca
    35101 cactactata agcaaaaagc gtgccaactt ttttacgata cactaattga atttaaagga
    35161 ttcttaacca atacacaaaa acgatacact ataaaagtgt atcgtttttg tgtattcatg
    35221 gcacagtgta ttctttttta tctaactgat tatggactag acgtagcaga taatacattt
    35281 cttctatcgg cacttttact tttatctgat cttccaacag aacaactgag gctgccaatt
    35341 ggctataaag aggatcattc gttgtatttg tcaattcgtt ttcttccaca gtcaatggtt
    35401 cattccgaat aatacgttcg atcattcctg ctgtgtgcat cactaggttg attttgaatc
    35461 catattcttc atcgccgatt cccgtcgtct gagagatttg tgtagaaaat tgccacattg
    35521 gatcgattaa cttactacca ttaataaaga tgaagttatc agagataaac tcaacacacg
    35581 tatttttagc tgactcttca tctagggaga tgaattcagt ttcctccaat tctgattcca
    35641 tcagcaactg atccaaaatg acttcgatat tttgatcaat aaaacgttct aaaggaataa
    35701 atggggcttg gatattcaaa tcagtaatcc cagtagtagc tatgatctga tattcttttt
    35761 ggataactgg cagttcttct ttcacttcca caatcgacaa agctagaact tctaattctg
    35821 tttcttgacg cttgtttacc gctcgttcga tcaattcttt gattctttgt gctgtacctt
    35881 cgccagatgc gcagatagcc aaaacagctt ttttcttatg aataggtttt gcttcctgaa
    35941 cagattctac agaagatatt tctgcgtaac ctcgaaaatt tttcaatgat tcatgcaaca
    36001 tatctagatc tgtccctaat actgagacct ttcgtacagt ttccaaaact agagaagttg
    36061 ttaccatatc aacagtacgt actggaattc cagtgtctct ttgaatttgc gaattaaacg
    36121 acgcaaggga ccccatatct actaagagta tgaccccgct tccttcatct acttcttgta
    36181 cattgcgttc aattcggtcc aatgctgttt tcggatccat atccaaaggc atgtctactg
    36241 ctcgtacgtt ctctgcatct aacaactgtt ggacaacttg gaccatactg cttgctgtgc
    36301 tgtttccgtg agctgcaacg actacaccga ttctgccgct tgcttgattt gcccgtaacg
    36361 agacgagtag aacggtcaag taatagtctt cactttctgg aatctccact tgaaaatacg
    36421 tttcgatata actacgcact tctttcgcaa cagcatattc atctggaaaa tcaaaagcca
    36481 tctcacggat gttatcattc gtatgccgtt cttctcctaa gttgattttt ttcaaaaaag
    36541 aactgatatg caagctcata gcataaataa agttttgctg aaactcatat ggtaaagatc
    36601 ttttgaccat tacataaatc tgattggtca cttctatgat tttcggatct acaaattctg
    36661 ctaatttgct gtctgcatta aaagaaaaac catggtcttt atagaaagac ttcagatgga
    36721 tgttgatatc tgtagaaatg aaatgattga ttgcttcttg atccaaacca tccgacttca
    36781 gtaaagctgc tttatctccg atgatatcat atagattata aggtaactca taggagtccg
    36841 attggatttt catgatcgta tcatttggtg ctacagttat ctttggctcc aatattttag
    36901 aaagctctgc cattgcactg cggttgcttg ctaattgaat caatccgcta cgaatccctt
    36961 ctgtcaaatc atcgattgtg atcgagattt cgggtgattg catatgattt agaaatcctc
    37021 gtgcacatac taactggata tttgacttca attgtcctat atttccatag gtcacactgc
    37081 cgatcaatgc ttttgctaca tcttctgtca aagaaatctt tcgctgcgtg cggtttgctt
    37141 cgtgggcaat catgattttt actaaatcga ctttctcatt tgcaggacgt ttttcaaaag
    37201 ccggaagttt gatattgatt gggatacgcc gaacaaaagt ttctaataga gaagaacctg
    37261 ggtcttctgt ggtcgcacct acgatccgta catttgcttc atgggatttc gttgtttccc
    37321 caagacgact gtaagttcca tgatccataa agtaaaagat catttcttga ccttcaggcg
    37381 gcaaccggtg aatctcatcc aaaaacagca taccgccatc cgcttgatcg ataatcccca
    37441 ttttttcttc atctgcgcct gtaaaagcac cttttgcgta cccaaataaa tgactcatca
    37501 aaagctcagg gttattggca taatcagcac aattgaaaac aatcagttcc gtttcctcat
    37561 ctattacatg atgcgctttt gcaaaatgat acatcgcgtg tgcaaaataa gtctttcctg
    37621 atccggtagg tcctgtaatc aaacaattta atcctcttgg cggatacaaa atcgcagctt
    37681 ttgcctgttc cactgaattt ttcatgctgc cgttagctcc aatgattttt gcaaagatat
    37741 ccttagtatc tatttggagt gtattttttt cttcagagac cggcgtttct ttgtaactga
    37801 ctacatgttt tgtaactggc tttacgtgcg taacgagtgt ttgatatatg taacgtactg
    37861 gtcgtccgtc tgtctttatc agttttcctt cgcgcactag ctgattcaga tctttactgc
    37921 tatttgttcg ctgaatccct aacacttcag caatttcctg tgttgttact ccttgatcat
    37981 attctgcctt tgttaaatga gcggtttttt ctttgacata catataaatt cggtcaatgc
    38041 gttttgccaa tggtatcgct ccttttctat agactattgt acttaggctt caaaacataa
    38101 tactatgtat ttatttctgt ttcgtcaact atttacctga ttttgttttg ttgataacac
    38161 gcggatagat agaaatgttc aatttaaaag aacgaaaaat cccgatacga tttttataat
    38221 ggaaacaaga aaaagaacac tgtttaccat ataattattt cactgatagc aaacaatata
    38281 gcttccatca attgaaaacg atagttatca aaacatcctg attccaaatt ggcaaaaaaa
    38341 tattaagtgt aaaaagacaa agtaaacttc gtagcctcca ctacaacttt tatttttata
    38401 aacaaaaaag gctgtgacaa aagtcttgtc acaacctctt taatcataag attcaattaa
    38461 gcttcttgag ctactggata aacagacact tgttttttgt tacggccttt acgttcgaaa
    38521 cgtactacgc catcaacttt tgcaaataaa gtgtcgtctc caccgatacc cacgtttaca
    38581 cctggataga tttttgtacc gcgttgacgg taaagaattg atccacctgt aactgtttgt
    38641 ccatcagcac gtttagcacc tagacgtttt gattctgagt cacggccgtt agatgtagaa
    38701 ccgccacctt ttttgtgggc aaataattgt agattcatag ataacaacat ggtctgcacc
    38761 tcctatttat cattaattgt tttaatttgg atatattctg agttttcagc ttctatcgat
    38821 tgtaatccta ataaaagatt ttctaataga atctcagcaa tattgttttg ttcttgtgtc
    38881 attcctgaag tgacttcggt ataaagataa ccgccttctt cttcattcat ttcaacaatc
    38941 ggctcaaaac cggctaatga ggcaatcccg ttgacagtgc tgatcgctaa cgcagaaaca
    39001 cccgcacaaa cgatatcact tccataagga ccagcatcag catgcccggt caaagtgaat
    39061 gaaacgatct gacctgcgtc atttcgctta aatgttcctt tgatcatata taggccttct
    39121 ttctcaaacg ctattaagcg ttgatcgcgt cgataaccac ttttgtatat ggttgacgat
    39181 gaccttgttt acggtgtgaa tgttttttag gtttgtattt gaaagtaacg actttctttt
    39241 gtttgccgtg tttttcaaca gtaccttcaa cagttgcacc tgcaacagtt ggagcgccta
    39301 cttttgtaga ttctccgcct actaagataa cttcgtcaaa aacaactttt tctcctgctt
    39361 caacgtcaag tttttcaaca tagattgctt gtcccacttc aacttttact tgtttaccac
    39421 cagttttaat aattgcgtac atgctataaa gcacctcctt ctgatatact tagactcgcc
    39481 atccgaagtg acagcatatg ctgtgcttat tacttgttct gagcggttgt agctgtggag
    39541 tccacaatta caacagtact agcttaccag aacagcaacc gttagtcaac taaatttaaa
    39601 atcaaaaagt aaaacaaaaa ttcaagcaat acttctgctt ttctatcctc acagttctac
    39661 caaataaaat agtcgcattg tgtttctttt attcttataa taaaagaaaa aggaggagca
    39721 taaatggaat acccaaaaga actgatacaa gaagcttctt taagaatgaa cggccgtcct
    39781 gtagaaggat ttatcgctgg gcaaggacct cttcatccta aactgatgct cgtgggagaa
    39841 gccccaggga aaacagagat tgatacgcat gttcctttta gcggtcaagc tggaaaagaa
    39901 ttgatgcagg cactttcttc aactggctta acacgagaag aggtgtatat caccagtgct
    39961 gtaaggagcc gcccgtatcg cgtgactcat cggatcaata aacggacaca acagtcagaa
    40021 actgtttatc caaatcggac acctactcgt tccgaagtat ttgcgcatgc accaatattg
    40081 gattacgaac tgatgaaggt ccagccaaaa ttaattgcga cacttggaaa cattggtctt
    40141 caacgtttac ttggcaaaga atatacggtt accaaaaatc atggtcaact ctatacaggg
    40201 cctgtcttac aattaacaga acaaaaggat gcctacgaat ggtcagaaaa aaactatcga
    40261 attcttccac tttttcatcc agcagctatt ttctacaatc gctcccttgt acctgttatc
    40321 catcaagact ggcaagtact agcttcactt ttacagcaat attaaaaaga acagacaaga
    40381 ccataaaatc ttcagtttac agtcttgtct gttctttttc attgttttaa tggctaattg
    40441 cagtatattt cgcatgtaaa gctgtcaacg catgagcaac tgtttgatgt tgatgcgtga
    40501 atgtatgttc ccacaagtga gaaagttgtt tttctacttc tggctgcaag gaagtataga
    40561 taatcgtttg tcctctttgc tctgcttttt cttgaaattc atcaaaaact gcacgtgccg
    40621 tcaaatcaat acttggtacg cctcgcatgg agaaaatgac accatcgtga tgatttagtt
    40681 cttccaattt atttttgagt ttttctgaag acataaaaaa cagtggacca ctaatataca
    40741 ctactgtcca gttattcagc ttttccgttt ctggcagccc cattctttgc caatcgatcg
    40801 tttctaatga aatcgtgata gcagcgcttt tgacaacaaa aaatacacag ccgccgatca
    40861 ccccgatcac gatagcaata cttaaatcaa agataactgt acaaatcatc gtaaggataa
    40921 acaatagaac tgctgaccaa tatttattag taaatagctc cttgatcgtt tcccattcat
    40981 tcatacgcca agccgtcaca atcaaaacac ctgcaagtgc tggcattgga aactgtgaca
    41041 tcactggcgc aaaaacaagc atggagagca gtaagacaac tgcatgtatc ataccagcta
    41101 atctagtttg tgcaccagat ttgatggcca cgcttgtacg ggcgatggcc gcagtagcag
    41161 gaatcccgcc aaagaaagga agcaacagat ttccaattcc ttgcgccatt aattcttgat
    41221 tgctatctaa gggcttattt gccattcggc cagccgaagc accacatagc aagctttcaa
    41281 tcattcctaa taaagcaatg ctaatagctg gaactgccac ttgttgcaag gcagaaaagc
    41341 taaaatctcc catattcaaa cgattactac tgatcaatgt ttgtggaatc ttacccacag
    41401 ttgctactgg taaatggaac aatagcataa ctgctgttgc caaaatgata gccagcagcg
    41461 aactaggcat tcgtttcgcc catttttttg gatagataaa catccctaaa atgactagcc
    41521 ctcccatgat caaagaggct gtgtttatcg gaaatcctat cgtttgataa ctacttagtt
    41581 tttcaataac gtttgtacct tccgagtgaa caccaaataa attatcgatc tgacctaatg
    41641 caataatgat agcaatacct gaagtaaacc ccgtaattac gggtgctggg ataaaagaag
    41701 tcagtgttcc taatcggaaa agtcctgctg caagtaaaaa tagacccgct aaaaaagtag
    41761 caagcaatac accttgcatt ccctttgttg ccgcaataga catcaagata gctgccatcg
    41821 cacctgtcgg acctgatatt tgataaaaac cgcctgataa accgccaata acgataccgg
    41881 caatgatagc cgtaatcaaa cctgcggccg catcagctcc gcttgatacc ccaaaagcca
    41941 aggctaaagg caatgctaca gcagctactg tgatcccagc caaaagatct ttctgaaatc
    42001 ttgaaagatc ataacctgaa aattcttttt ttagtgagga aacataattt ttgaacacct
    42061 atctaactcc tttttcatga aattttctaa aataccatt
//
LOCUS       DASJSZ010000014        41704 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.014, whole genome shotgun sequence.
ACCESSION   DASJSZ010000014 DASJSZ010000000
VERSION     DASJSZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.014"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="U4B03_000731"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="U4B03_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEN2067605.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="U4B03_000732"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="U4B03_000732"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEN2067606.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="U4B03_000733"
     CDS             complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="U4B03_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEN2067607.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="U4B03_000734"
     CDS             2179..2499
                     /locus_tag="U4B03_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEN2067608.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3480)
                     /locus_tag="U4B03_000735"
     CDS             complement(2611..3480)
                     /locus_tag="U4B03_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2067609.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="U4B03_000736"
     CDS             complement(3576..4880)
                     /locus_tag="U4B03_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2067610.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="U4B03_000737"
     CDS             complement(4877..5386)
                     /locus_tag="U4B03_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067611.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="U4B03_000738"
     CDS             complement(5561..7696)
                     /locus_tag="U4B03_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2067612.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="U4B03_000739"
     CDS             complement(7779..9434)
                     /locus_tag="U4B03_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2067613.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="U4B03_000740"
     CDS             complement(9436..9750)
                     /locus_tag="U4B03_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2067614.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="U4B03_000741"
     CDS             9920..10180
                     /locus_tag="U4B03_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEN2067615.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="U4B03_000742"
     CDS             10194..10508
                     /locus_tag="U4B03_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEN2067616.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="U4B03_000743"
     CDS             complement(11238..11792)
                     /locus_tag="U4B03_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN2067617.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="U4B03_000744"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="U4B03_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="U4B03_000745"
     CDS             complement(14153..14869)
                     /locus_tag="U4B03_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN2067618.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="U4B03_000746"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="U4B03_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEN2067619.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="U4B03_000747"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="U4B03_000747"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEN2067620.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="U4B03_000748"
     CDS             complement(18339..19799)
                     /locus_tag="U4B03_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2067621.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="U4B03_000749"
     CDS             complement(19789..21084)
                     /locus_tag="U4B03_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2067622.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="U4B03_000750"
     CDS             complement(21416..22093)
                     /locus_tag="U4B03_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEN2067623.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="U4B03_000751"
     CDS             complement(22123..23214)
                     /locus_tag="U4B03_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEN2067624.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="U4B03_000752"
     CDS             complement(23315..23749)
                     /locus_tag="U4B03_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2067625.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="U4B03_000753"
     CDS             complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="U4B03_000753"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2067626.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="U4B03_000754"
     CDS             complement(25787..27682)
                     /locus_tag="U4B03_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2067627.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="U4B03_000755"
     CDS             complement(27740..29515)
                     /locus_tag="U4B03_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN2067628.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="U4B03_000756"
     CDS             complement(29565..31061)
                     /locus_tag="U4B03_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2067629.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="U4B03_000757"
     CDS             complement(31255..32877)
                     /locus_tag="U4B03_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN2067630.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="U4B03_000758"
     CDS             complement(33205..34518)
                     /locus_tag="U4B03_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN2067631.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="U4B03_000759"
     CDS             complement(34763..34987)
                     /locus_tag="U4B03_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067632.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="U4B03_000760"
     CDS             complement(35007..35429)
                     /locus_tag="U4B03_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN2067633.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="U4B03_000761"
     CDS             complement(35571..36281)
                     /locus_tag="U4B03_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067634.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="U4B03_000762"
     CDS             complement(36299..36796)
                     /locus_tag="U4B03_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2067635.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="U4B03_000763"
     CDS             complement(36833..37285)
                     /locus_tag="U4B03_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2067636.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="U4B03_000764"
     CDS             complement(37420..38025)
                     /locus_tag="U4B03_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2067637.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="U4B03_000765"
     CDS             complement(38475..39095)
                     /locus_tag="U4B03_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2067638.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="U4B03_000766"
     CDS             complement(39097..39966)
                     /locus_tag="U4B03_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEN2067639.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="U4B03_000767"
     CDS             complement(39989..40396)
                     /locus_tag="U4B03_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN2067640.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="U4B03_000768"
     CDS             complement(40415..41704)
                     /locus_tag="U4B03_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2067641.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11759 a         8983 c         7021 g        13941 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccat
//
LOCUS       DASJSZ010000015        40186 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.015, whole genome shotgun sequence.
ACCESSION   DASJSZ010000015 DASJSZ010000000
VERSION     DASJSZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.015"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..434)
                     /locus_tag="U4B03_000769"
     CDS             complement(<1..434)
                     /locus_tag="U4B03_000769"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEN2067642.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
     gene            680..1627
                     /locus_tag="U4B03_000770"
     CDS             680..1627
                     /locus_tag="U4B03_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2067643.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(1838..3910)
                     /locus_tag="U4B03_000771"
     CDS             complement(1838..3910)
                     /locus_tag="U4B03_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2067644.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(3843..4220)
                     /locus_tag="U4B03_000772"
     CDS             complement(3843..4220)
                     /locus_tag="U4B03_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEN2067645.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(4349..4927)
                     /locus_tag="U4B03_000773"
     CDS             complement(4349..4927)
                     /locus_tag="U4B03_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2067646.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            5059..6351
                     /locus_tag="U4B03_000774"
     CDS             5059..6351
                     /locus_tag="U4B03_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2067647.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(6411..7529)
                     /locus_tag="U4B03_000775"
     CDS             complement(6411..7529)
                     /locus_tag="U4B03_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEN2067648.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(7543..8964)
                     /gene="arcD"
                     /locus_tag="U4B03_000776"
     CDS             complement(7543..8964)
                     /gene="arcD"
                     /locus_tag="U4B03_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEN2067649.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(9210..10331)
                     /gene="mnmA"
                     /locus_tag="U4B03_000777"
     CDS             complement(9210..10331)
                     /gene="mnmA"
                     /locus_tag="U4B03_000777"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEN2067650.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            10735..11334
                     /locus_tag="U4B03_000778"
     CDS             10735..11334
                     /locus_tag="U4B03_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEN2067651.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(11472..12758)
                     /gene="efmA"
                     /locus_tag="U4B03_000779"
     CDS             complement(11472..12758)
                     /gene="efmA"
                     /locus_tag="U4B03_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEN2067652.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(13029..13373)
                     /locus_tag="U4B03_000780"
     CDS             complement(13029..13373)
                     /locus_tag="U4B03_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEN2067653.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(13510..14655)
                     /locus_tag="U4B03_000781"
     CDS             complement(13510..14655)
                     /locus_tag="U4B03_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2067654.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(14835..15809)
                     /locus_tag="U4B03_000782"
     CDS             complement(14835..15809)
                     /locus_tag="U4B03_000782"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN2067655.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(15889..16764)
                     /locus_tag="U4B03_000783"
     CDS             complement(15889..16764)
                     /locus_tag="U4B03_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEN2067656.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            16894..18801
                     /locus_tag="U4B03_000784"
     CDS             16894..18801
                     /locus_tag="U4B03_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEN2067657.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(19342..22620)
                     /locus_tag="U4B03_000785"
     CDS             complement(19342..22620)
                     /locus_tag="U4B03_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEN2067658.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(22891..23436)
                     /locus_tag="U4B03_000786"
     CDS             complement(22891..23436)
                     /locus_tag="U4B03_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2067659.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(23448..24140)
                     /locus_tag="U4B03_000787"
     CDS             complement(23448..24140)
                     /locus_tag="U4B03_000787"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEN2067660.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(24159..24443)
                     /locus_tag="U4B03_000788"
     CDS             complement(24159..24443)
                     /locus_tag="U4B03_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067661.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(24456..25019)
                     /locus_tag="U4B03_000789"
     CDS             complement(24456..25019)
                     /locus_tag="U4B03_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2067662.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            25247..25909
                     /locus_tag="U4B03_000790"
     CDS             25247..25909
                     /locus_tag="U4B03_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEN2067663.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            26033..27229
                     /locus_tag="U4B03_000791"
     CDS             26033..27229
                     /locus_tag="U4B03_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEN2067664.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(27330..28706)
                     /locus_tag="U4B03_000792"
     CDS             complement(27330..28706)
                     /locus_tag="U4B03_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2067665.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(28743..29610)
                     /locus_tag="U4B03_000793"
                     /pseudo
     CDS             complement(28743..29610)
                     /locus_tag="U4B03_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(29699..30724)
                     /locus_tag="U4B03_000794"
     CDS             complement(29699..30724)
                     /locus_tag="U4B03_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2067666.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(30742..31416)
                     /locus_tag="U4B03_000795"
     CDS             complement(30742..31416)
                     /locus_tag="U4B03_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2067667.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(31600..32493)
                     /locus_tag="U4B03_000796"
     CDS             complement(31600..32493)
                     /locus_tag="U4B03_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2067668.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(32567..34264)
                     /locus_tag="U4B03_000797"
     CDS             complement(32567..34264)
                     /locus_tag="U4B03_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2067669.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(34257..35570)
                     /locus_tag="U4B03_000798"
     CDS             complement(34257..35570)
                     /locus_tag="U4B03_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN2067670.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(35570..39607)
                     /locus_tag="U4B03_000799"
     CDS             complement(35570..39607)
                     /locus_tag="U4B03_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN2067671.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(39620..40078)
                     /locus_tag="U4B03_000800"
     CDS             complement(39620..40078)
                     /locus_tag="U4B03_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2067672.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT        11320 a         8719 c         7174 g        12973 t
ORIGIN      
        1 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
       61 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
      121 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
      181 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
      241 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
      301 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
      361 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
      421 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
      481 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
      541 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
      601 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
      661 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
      721 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
      781 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
      841 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
      901 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
      961 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
     1021 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
     1081 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
     1141 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
     1201 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
     1261 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
     1321 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
     1381 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
     1441 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
     1501 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
     1561 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
     1621 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
     1681 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
     1741 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
     1801 agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt gctgctttta
     1861 gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt ttcagcaagc
     1921 gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc aatttttctg
     1981 tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg atttccattt
     2041 tccggtgaca ttgcgggcat cgatgattag agacttttgg atcttttctt ctgcggtagt
     2101 tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc tcgcgcagat
     2161 tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat tcttgtttgc
     2221 ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc aagaaggttc
     2281 ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt tctgtcaaat
     2341 ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta ggagatactt
     2401 gcagtccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg ataatctctg
     2461 cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag cttgcttctg
     2521 acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga ttgattctca
     2581 ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc catcctgctt
     2641 ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc gtaacttttg
     2701 tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca atcatttgat
     2761 agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca gttggcaaaa
     2821 gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg acgatggctc
     2881 cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc aatcgttctt
     2941 tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga taagtaacga
     3001 tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca gaatagccat
     3061 agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc gcagattccg
     3121 tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc aattgaacaa
     3181 gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc attttggctt
     3241 gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac tgctcgattt
     3301 tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct gctgataaat
     3361 tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc cagtccgctt
     3421 ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt cttagttgct
     3481 tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc ttcactggtt
     3541 tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct cttccggcat
     3601 ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg attgctttta
     3661 attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc attggcaacg
     3721 tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc aatcctaata
     3781 aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt ttgtgctttt
     3841 gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc gctagaatta
     3901 gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt tttcaacaac
     3961 gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg ctcatctccg
     4021 tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata attttgatgg
     4081 atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc gaatgtttct
     4141 tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt gtccccgaac
     4201 atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga ctcatcttag
     4261 tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta atgaacatct
     4321 taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag ttattgatca
     4381 tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac
     4441 gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga actttttctt
     4501 ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt tcatggattc
     4561 catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca tacactgctt
     4621 gatttgaaaa agcaaaggat cgataagttt ctgcatagat ctgcagtgct tcctgaccac
     4681 tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt ttcgctgtta
     4741 gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga gccttgatat
     4801 ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct tctgctagtt
     4861 ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt tccatgtttc
     4921 ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag agaaaaaaga
     4981 gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga tgaaaatttt
     5041 cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag gaaaagagaa
     5101 acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg tatcggattt
     5161 agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta tagtacgtca
     5221 caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac tacgcttatc
     5281 tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct gttgcgtgct
     5341 tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt gtatcaatta
     5401 gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc cactgccctt
     5461 gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt agcaactggt
     5521 tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc agcgtttggc
     5581 tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt gttgagtctg
     5641 tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag
     5701 attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac aacgatgatc
     5761 atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg ccaattgctg
     5821 catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc tgggattgcc
     5881 actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag tgaaagaata
     5941 cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt cgtcacgaat
     6001 gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg tctgctccct
     6061 gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg gatcttcagt
     6121 tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg ctccagtgtt
     6181 tccagtgcat ttggttatcg cggcgtcttt ctttcgactt ctcttcttgt tttggtcaac
     6241 ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc agaagaaaaa
     6301 gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata agcgtaaatg
     6361 aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt
     6421 tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt tttacaacag
     6481 taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa tagtactcct
     6541 gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc taatttaatg
     6601 aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt ggctacccat
     6661 tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa tattttttct
     6721 ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca aatcatcgtg
     6781 tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt ccagccaata
     6841 cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc tttttcatag
     6901 acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac ttcatctatc
     6961 aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt tttccgatct
     7021 agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg
     7081 actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt caaatgaaca
     7141 aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata agtaggaagc
     7201 atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag attagcacca
     7261 gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag agaagcgact
     7321 tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc gtttttgcgt
     7381 ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc taatgtcaat
     7441 gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt atttagccat
     7501 tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct tgatccagcc
     7561 tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa tcattacttt
     7621 ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga agcaaaaacc
     7681 aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa tgatgcccat
     7741 caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc cttcttttaa
     7801 agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg ccgctgtaca
     7861 taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata agaatagatt
     7921 agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat tcgtttttcc
     7981 ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg ctgggagcat
     8041 cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga tcagcgctgc
     8101 tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg aaatagttgc
     8161 taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat agataaacaa
     8221 taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt
     8281 ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca tcaaacaacc
     8341 tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat tatcagatac
     8401 gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata caaatattaa
     8461 aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga cgctcgctcc
     8521 ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg caaataaaat
     8581 agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg cgctcatcag
     8641 catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc aaccactgat
     8701 gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac catctagatc
     8761 tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac caatcccaac
     8821 gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat cacttgtgat
     8881 cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc cccatttgct
     8941 aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg aacaaatcac
     9001 ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc aactttattc
     9061 atattattgg atattatgaa ctttttacac aagtatttca agtataaaat gaataaaaat
     9121 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
     9181 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
     9241 tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa tacaactgct
     9301 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg attatcatcc
     9361 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
     9421 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
     9481 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
     9541 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
     9601 cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc accatcaaga
     9661 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
     9721 tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
     9781 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
     9841 agctgttctt gtgataattg gctaaggaag taagtttgat ctttattatt gtctactcca
     9901 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
     9961 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
    10021 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
    10081 aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat cccgatttga
    10141 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
    10201 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
    10261 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
    10321 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
    10381 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
    10441 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
    10501 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
    10561 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
    10621 cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag atgttatgag
    10681 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
    10741 tactttatca gtgatatgca ctttttccat gaacgcttat taggaaaaaa cgactttgct
    10801 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
    10861 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
    10921 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
    10981 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
    11041 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
    11101 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
    11161 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
    11221 gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac tccgttatct
    11281 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
    11341 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
    11401 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
    11461 tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt tttctgtaat
    11521 tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca taataaaatc
    11581 ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
    11641 aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc taagacacga
    11701 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
    11761 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
    11821 tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
    11881 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
    11941 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
    12001 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
    12061 ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc aaattttgca
    12121 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
    12181 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
    12241 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
    12301 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
    12361 ataatcgact ggatcgttgg catttggaat gtttgtgcga ccgaacgcat aaacaaagac
    12421 acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc taaaataagc
    12481 gcgaaaatcg ctacaataat atccgtgaca atcaaaagag cttttttatc ccagcgatcc
    12541 actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag taaagtagca
    12601 aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat cgcatattgg
    12661 acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa ataatttctc
    12721 ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct tttctttttc
    12781 attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa aaaatacctt
    12841 tttccctaaa taacaatcgt ttcaatttaa atttctttac ttagaaacaa taaatcagca
    12901 aaatattaaa aataacaaca tgtgttaaat ttaaaaataa aaaagacaac taatttgcgt
    12961 cgtccttaca aagttagtca tctaacaata ggccgtttca aaactagttg tctttttata
    13021 tccacagatc attcgacttt ttcgaataac ccacggctaa tcatcccatc catcagaaaa
    13081 taaaatttat cttggctcat tttctgtttt ggatctttga aaatattgac tgctttcaat
    13141 ccatttgctg tagtgatcga cattttcaat gtttgaatat cttttgatac agtgattttt
    13201 aatttgaatc cttctttgct ttgcggcgtg gcatctaatg gtcggatcaa ttgataattg
    13261 cctccgtttg tttctgtaaa tccattatcg cgcaatgtat atctttttgc atcaggatgc
    13321 aaacgatagc taattggtgc gcccagtacc gttgctgttg tttcaaatgc catgattttc
    13381 acctcgataa aattagtttc tctttattat aaaagtttat cctgattttt actaggtttt
    13441 tcagttaagt tccctaaaaa atgaaggcgt tctctcagac atctgttcat ataagaatga
    13501 cttcactagt tattttttga cagcacgaac taattgatca aggaaatact ggatgtcgtc
    13561 ttcggtattt cccaaaccaa agctgatacg gatggattct tttagtatcg gtgtatcttc
    13621 tccatacatt gcttctaata cgtgtgaagg ctcgatattt cctgctgtac aagctgaacc
    13681 agtagaaata gcgaccccat taagatcaag cttcatcagt agcaaatcat ttgcttcgcc
    13741 aattaagcga agattcaaca catgagggag tttattcgtt aaatcaccat ttacttgata
    13801 agggatttcg tttgcttcta atgtatcaag aatcaattgc tggaatcctt tatatttgtt
    13861 gttacgtgct tcacgttctt caggtgacag cagttcaact gcttttgcca taccccagat
    13921 ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc tgctccccgc ctaaaagcaa
    13981 tgcagggata tttactccgt ctcgtttata taagaaaccg acgccttttg gcccattgat
    14041 tttatgagca gagatactta ataaatcgat tcctaattct tctggatgga tcgtttggtt
    14101 tccgtacgct tgtaccgcat ctgtatggaa ataagctgga tgatccgcta aaagttcacc
    14161 gatttctttg attggcatca aatttccgat ttcattgttc ccgtacatca cagagaccaa
    14221 gatcgtatct tctcgtaacg cttttttgac ctcttcgatc gacagattcc ctttttcatc
    14281 tacaggcaga tatgtaacgt caaacccctt tgtctctaaa tatttcatgg tattaagaac
    14341 agcaggatgt tcgatagctg tagtgattag atgtctgcct tctttttgac gcgagaacgc
    14401 tgtttctaag atagctgtat tatccccttc tgttccccca ctgttaaaga tgatctcgtg
    14461 aggttttgct tgcagactgt ctgcaatgat ttgtcttgct gtttccaatt tttcatgcgc
    14521 gtggcgacca aatccatgta tgcttgaagg atttccaaac gtgtcttgca tgattgccgt
    14581 catcgcttca attacttgtg gatgcatggg ggtagttgcc gcatgatcca gataaatagt
    14641 tttcacttca ttcatccctt ccaaattatt gctaggcaca ttctagcata aaatagcgaa
    14701 caaaccaaag aagagctcat ttttgtctag aggacgtttc atgggcatag aaaaaaggag
    14761 ctgtgacaaa agtcttgtcg cagctccttg aagctgctca cttctgtcac aacttctttc
    14821 cctctttttt ttgtttattc gaattctttt tcttccagac ggaataacgg actcatcggt
    14881 gtattttcat ggatgcgttt gaccgcttca cccattaatt ctgaacatgt aacgattgtt
    14941 aagttttctg gatgacgatt ttcttctgtg aaaacagaat cagtgataca aatatctttg
    15001 attggtgcat tgtctaaaat acttttcgca tgatcggaca ataatccatg tgacgcacag
    15061 gcatatactt cttttgcgcc gttttctttt aatacatttg ctgctcgtgc aagcgttgct
    15121 cctgtgttca agatatcatc taccatgata catgtctttc cttgaacatc accgatcaca
    15181 taaccaccct cagttgaagc atcatctgca tgatctacga tggctaatgt gctgttcaaa
    15241 tattctgaaa gactacgtgc acgctgaaca ccgctgtttt ttggcgaaac aactacgatg
    15301 tcatctcccg tcagacctaa ttgacggtaa taatgagcaa acaacggcat cgtaaataaa
    15361 ttatctacag gaatatcaaa gaatccttgt acttgaaccg tgtgcagatc taaagtcagt
    15421 actcgagttg cccctgcttc ttcgatcaag ttagcgatca atttcgcagt gatcggttca
    15481 tgaggtttcg ccgtgcggtc ttgcctagca tacccatagt aaggcaatac gacattgatc
    15541 gttttcgcac ttgctcgctt cattgcatcg atcatgatca gtagttccat atagtaatca
    15601 ttgacaggtg cattcgttga ttgaacgata tacacatgat cccctcgaat actttcttcg
    15661 atattgatgg aaatctcgcc atcactaaat tgcttcacga cgcttttacc taattctgtt
    15721 ccgaacactt tcgcgatttt ttcagctaat ggacggttgc cattcaaact aaaaatcttc
    15781 aacgtgtcat cctgatactt attactcatg tagtcctccc caaaacttct ctattcttcg
    15841 atagtttaac atatttttcc aagattttct gttaatttca gcaaattctt aaggaagtga
    15901 aactgttccg ttactccaac tggcaggaat gactttatga taatcgcccc agttgtcgtg
    15961 tactttgtaa taacgttctc ctgtttcagt ttcaaaataa gcgtacgctg tcacccaatg
    16021 attgccatat gtactgccaa gtactttcaa aataccaatc atcacaggtt tgccttcacg
    16081 tatacgtttt gtagctcgct gccatgaacc tagaagagtt gctcgtgcac gctcatgatt
    16141 gccattcttc tttaaaaaac tagaaatccc tgtactaacc tgaaaaggaa tcgtaggaag
    16201 tcctagtggt tggatctggc tacgcaatgc cttcgtcaaa accaacgaat cagccgtatt
    16261 tttcttgcga atctcatttg gaagcatcca ccccttacga tagtcttgat aataggcaag
    16321 gagaactgct gccgcgtaag taccacaaag atagcctgag ggggtagccc atttcttata
    16381 cctcaaaaaa cgttcttctg gcaatcccac ccagcaattt gcgagttcct ttcctgacct
    16441 tttttcttcg atcaaaacgg gaatatcttc ttttctgttg atccatctag ataactcgat
    16501 tggctcttca ttgatcagct tccatttttt acaagaaaga ccctcttgat aagtatattt
    16561 tgctggagaa tattctaccg gatgtcctga ttgatcgata atcacataac tttgatcttc
    16621 aaaaaccaga gctaaaaaaa gcaatttact ttttcttgac tgcagctgct gagtgctggc
    16681 taatttttta atttttttat ctactttgaa atataaaggt ttatccacta cttgattcag
    16741 ttgggatcgc ctcctttttt tcattataag tgaaggattg aaaaaaacaa atcagtttga
    16801 ttgttcgaat tttcaatttt ttatgttcta tttgttaatt ccttcatttt gtaagataat
    16861 gggcacacga aacaaaaaag gagcgatcga tgaatgaaca aagaacgatt gaaacaagat
    16921 ttctatgaag cagtcaatga agaatggcaa aaaacagcaa aaatccctgc agataaacca
    16981 gctacaggcg gtttccaaga tttagttgac gggatcgacc aacttttgat gggagaaaca
    17041 gacgatctgc ttgcccatcc agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca
    17101 tattatcaac tggcaaacga ttatgaccga agaaatgagt tgcatgctca accgcttcaa
    17161 cctataatcg aaaaagttga atcattgcag tctttcgacg aattgaataa gcaatttcca
    17221 gaatggctgt tggatacatt accattgcca tttaatctgg atgtagatgc agatatgaag
    17281 aatgcgcaaa cgaacgcctt ctttgcttat ccgccttctt tattccttcc agataagacg
    17341 tattatgcat cagaccatcc caacggtgca caactaatag gcgttttctt tgatatggct
    17401 gttcagttat tagagcttgc cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa
    17461 gcagtgcaat ttgataaatt gattgctcct catgtaaaaa gtgcagaaga aaacgcagat
    17521 tacagcaaaa tgtataatcc gcaatctttt gatacattta ttaaatacac agataaactg
    17581 gacttgaaaa aacttgtcac gggtttgatt gggacaactc cagataaagt gatcgtaact
    17641 gatccagtgt acttcgatca tttgaatgac atcctgacag aagctcattt tccgttattc
    17701 aaaagctgga tgatcattaa aacagtacgt tctcttagtg gttatctatc agaagaattc
    17761 cgccaagtca gcggaatcta ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag
    17821 aaaaaagcag cgtattacct tgcctctgga caattcgatc aagtagttgg cgattactac
    17881 ggaaaaaaat attttggcga agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg
    17941 atcgccgttt ataaaaaacg tctagaagaa aatgactggc ttggggatgc aacaaaagaa
    18001 aaagccgtca taaaattaaa taacttaggt atccaagtag gctaccccga taagatccca
    18061 gcattattcg accagttcaa aacagtacct gcagatcagg gtggtacttt gctttcaaat
    18121 actcttgttt tcagcaaact cacattaaaa gatcgtttca gcaaatggaa caaaccagtg
    18181 gatcgttcag aatgggaaat gagtgctgac acggtcaatg cttattatca tccatttcgg
    18241 aatgtgatcg tctttccggc agcgatcctt caagcaccat tttacagtct agaacaatca
    18301 agctctgcta attatggtgg aatcggagct gttatcgctc atgagatttc ccatgcattt
    18361 gataataacg gcgctttatt tgacgaatat ggcaacttga acaactggtg gacagaagaa
    18421 gatcttacac aatttcaaaa caaagcacaa gcaatgatcg aagaattcga tggtctgcct
    18481 tttgctgacg gaaaagtaaa cggaaaatta accgtttcgg aaaatattgc tgatgcaggc
    18541 ggtctaagct gtgcattgga agcagcaaaa acggaatcag atctttctat cgaggaattc
    18601 tttgtcaatt gggcaacgat ttggcgtaca aaagcaaaaa aagaatacca acaactttta
    18661 ctgcaaatcg atgtccatgc ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt
    18721 gacgaattct atgaaacatt tggtatcacg gaacaagacg ctatgtatct agcgccagaa
    18781 aaaagagtgc gaatctggta agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa
    18841 ggaatttggg aaaatcgctt ctcaaatttt tgtgacattt cggcttattt ttcgaagaag
    18901 ttacttctgg aataccatgt attagaggtt gtgaaaaagc tgcttagttt caagtagtaa
    18961 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta ctaccgagaa
    19021 actggctttt gaacaccgtt tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt
    19081 agagaaaaaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgagg
    19141 gtcagctgat tgaacaccgt ttagtggaga gattgtgaaa aagctgtcct gcatcaagta
    19201 ataagaacag ccaatcaaaa gcagcaagga gaacctcatc tataaaaaga tagaaggaag
    19261 tgagacatct attggtctca tttccttcta tttttacttt cctcagtcaa ttgttttaca
    19321 tcataaaagg agaaattcca cttatttttt tcgctgtttc atgacataaa caactacacc
    19381 gattaacaaa attcctccag ctaatgctag caacgggtta ttaaattctc ccgtttttgg
    19441 gagtccttga gtgccatttt tttcactttc ttttgctgtc ctatccggat gagaaggttt
    19501 tccatttggt tttccatttg gtgattcatt tcctggattt tctctttctg gtttgtcatt
    19561 tcccgggggc acatcggcag tttctcgcac agtgatcgct ttttgctggg taacagattc
    19621 tgtatgagca gaccattttt ccccatcaaa tatttccgaa cgataagtga cttctagcgt
    19681 atattctcct tttgaaggtg ctgaaaaaat agcggtatga ttagaaaatt gagcacttga
    19741 ctgtgtttca acgatttgga tcttttctgg gaaataacgc atatcttcaa agacaggcgt
    19801 tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt
    19861 tccttctgca ttctccgcca caccatttgc ttcttgtata ataatcggat tattttgttc
    19921 atttggtttt gtctgatttg gttcttgtac gattgcatac tctggatatt ccgaagacgg
    19981 tgaggtttct ccgtcaaaaa tagcacctac agataatgga agaccatcca ctctttcctt
    20041 ttctgtttga cgattccccg gtttaatctg catcagtcca tgaacatgga tacttccatt
    20101 ttcatagcgc gtaacaggct gctgaccttc tttcgaacat gtatttagag actcgatcca
    20161 atcttttgat atggtatgtc caagagtatt ttctgattgt ttctctcgtt cattccaata
    20221 gtagttcaac gagtggtaga tttgatcttc gttttgattc aatgggcgga tctgctctct
    20281 catctcaaga ttctctgttc ggaaagataa gataccagaa gcatggtagt ctgtgtttcc
    20341 tgctgaagag gtataaagag ccacattata tgaaccgtta ttaaaacttg tactgctatt
    20401 gacgataata tctgttccgc tattggaatc gattcctttc gcaagatttt cgaaagacaa
    20461 actattgatc aattcatgag ggcccgttaa agaagaaccg cccattttga atccattacc
    20521 gttaccttct ccctcaattc ctggtaccca gccgtttcga taagcaactg agtttttgat
    20581 gattactttt ccgatcgaac ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc
    20641 attatgatac gcgatacaac cgtcaaatac atttccttct cctattgtca gtttggccgc
    20701 aaatccatcg gcatcttcaa atcctggatc ggcattttca aaagatgtac agttcttgac
    20761 taagttatgc gcgggccatg cttcgaaagg atcatttgag ctaccgctga tttggattcc
    20821 tgtatttcca ttccgataag catgaatctc ttcgatcaaa ttatgatttc cagaaatctg
    20881 taatcctttt tgcatagctg cactatttgt aatgtcaaac cctcgtaaac cccagtagtg
    20941 actgaacagt gtcatcccat ttccagttcc tttaaaatca agtacaggac gcttgttttc
    21001 tggatttttc tctgcaatca actgaatcgg gttttctctt gtcccgttaa cgccttttgg
    21061 aatcgtcaat cctctttcga aggaataagt tccaggcgcc atgacgattg tctgcccagc
    21121 atatgcgtat ctcaatgctt gtgtcagaga taatggattt tgtctgcttc cgtttcctgc
    21181 atctgaccct tctggtgtta cataaactgt cgaatattta ggtttacggt agtccactgt
    21241 atgaagaatc tcttttgttt cataactagt catctctacg tattcttctg gcggatagtt
    21301 tttatctggc gtaaaactga aagtaaattc gttcgttcct tccatcagtt ttgtctgata
    21361 atcaaattcc cgattcgcag tgatagccaa gttttttgcg ttcttaagca cctctcctgt
    21421 tgctttattt ttaattgtca ataatccgtc agcatttgct ctaaaggaca tagtatgatc
    21481 ttggtttcct gaatgattac tagaggtcac agtgtaaatc ggatcaatca agcgagtagg
    21541 tctttcttgc ccaggaggat ctgtcgcagg atctgagtaa gcaacttcga ttccttctac
    21601 cttgatcgtc atattccttg ctgcagcaaa tcctgcataa ataaccgatt catctgaagc
    21661 gtataatttt tcccagtcat acatgatgtc agaagccatt tcttctcctg tttcaggatc
    21721 gtaataatat gcctctaaac cagtattcgt ttttttcatc tctacgtcca gtttagtaat
    21781 cgagttcgtg gccggaaaca gattcccatg accgtttccg aatatattat aagaacctgt
    21841 ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt gtttccactg agacactccc
    21901 aggagctggc gcagcttgcg gatcttcaga tgatattcct gtgattgccc gtgtccccaa
    21961 tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc acttcttgtc tatccgagtc
    22021 ccagacatat tcgatccgac tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc
    22081 aaacaaatga ttttttggta ctgcgtctct taccattaaa gcaaatcctt cttgggcatt
    22141 cgtataggtc caagattcta tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg
    22201 gattgcttga taataatagg tcattccatc tgatccgctt gactggaatt ttccgccgtt
    22261 attcgtggaa gagagccgca cgccgtcttc taatggttca atggcatacc tactagcact
    22321 tgtgctttct ccgaaaacaa cccctttcca ctcttcattt gcttcttcag attgcttttt
    22381 tgtcttttca ttttctgatt ctttcatcat aggatcattt ccttcttccg tatccatatc
    22441 ttcactagtt cttggtattt cctccattgt tgacatccct tcaatgttca cgtgtgcatt
    22501 ttcttcgttc acagactgtt gttcgttagc agcaacttca acgacagaaa ttaaaaccgg
    22561 ggtactgagc ataaggagat tcatcattag cgcactgtat tttatacgtt tttttaacaa
    22621 gtgtattcct cccgtaacaa attagaaagc gtttacttaa agtattacct aatttatttt
    22681 tactgtcaat aaaaaacaaa attataataa gtgtatttta taaaaagaaa aaatataact
    22741 taagttgttg ataataaaga gatttattaa cgtaaaaaga ataagttttt ttaaaataac
    22801 gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc tcttctttta ttattttgtg
    22861 ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
    22921 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg aatctttgaa
    22981 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
    23041 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
    23101 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
    23161 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
    23221 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
    23281 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
    23341 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
    23401 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
    23461 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
    23521 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
    23581 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
    23641 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
    23701 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
    23761 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
    23821 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
    23881 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
    23941 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
    24001 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
    24061 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
    24121 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
    24181 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
    24241 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
    24301 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
    24361 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
    24421 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
    24481 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
    24541 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
    24601 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
    24661 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
    24721 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
    24781 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
    24841 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
    24901 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
    24961 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
    25021 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
    25081 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
    25141 tctttagtcc gatactcttt ctagtagtcc gtatgctaaa ataagtatgt aatcgaataa
    25201 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
    25261 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
    25321 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
    25381 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
    25441 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
    25501 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
    25561 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
    25621 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
    25681 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
    25741 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
    25801 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
    25861 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
    25921 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
    25981 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
    26041 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
    26101 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
    26161 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
    26221 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
    26281 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
    26341 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
    26401 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
    26461 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
    26521 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
    26581 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
    26641 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
    26701 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
    26761 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
    26821 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
    26881 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
    26941 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
    27001 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
    27061 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
    27121 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
    27181 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
    27241 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
    27301 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
    27361 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
    27421 acagtttgca gacgaagcat caatacaata caagtcaaaa tggagacgat tggcaatagc
    27481 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
    27541 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
    27601 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
    27661 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
    27721 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
    27781 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
    27841 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
    27901 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
    27961 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
    28021 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
    28081 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
    28141 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
    28201 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
    28261 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
    28321 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
    28381 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
    28441 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
    28501 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
    28561 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
    28621 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
    28681 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
    28741 gattaaactg ttttcatata ccctaaaagg gataccacct aaaatgacta aaaggcaacc
    28801 aacaataaca taaatgagct cttttttcgt ttttctttcg ccaaggatga aaatcccacc
    28861 taaagtagag ataatgatcc ccatttgtga aagtgagaag ccaacagcta aaccaagtga
    28921 tttgactgtt agaagcatgc agatgtttcc taagccccat aataatccac agatcatgtt
    28981 tttccacaca aactgatcga ctttaacttt acggaaagca aagaagcttg cgcctaagat
    29041 cattccgata ctttgaggta agataattga catcgcatcc aaattcgccc agttgacgat
    29101 gatggtatat acaccataac caatcgtaga gaagatcaat gctctaaaac ccttaccaaa
    29161 atcaagcatt ttattatctg tatctatttt tccttcgtca tcttgtcggg cagtcaaata
    29221 cgcaccgctt acaagaagag ctaaagcaat aataccgtat acataatctt tcgtttgtgt
    29281 ccattcatgg aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa aaccagtaga
    29341 caatggtagc cctacagaaa cacccatgta cttcattcca tggaactgtc cattttgtcc
    29401 aacactccaa gctaagccgg ataaaaatcc gatcaagaaa atccaagggg tgattacagg
    29461 actaacaacg aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt
    29521 ttgttgattg gcatcgccac caattttacc acttactaaa ccaatactac cccatgccaa
    29581 catagggact aatgctatta aaatttccat aaccaatctc cttttatttc acatgataag
    29641 aaacttatca gcgaaaacgt taacaacaca agcacttttt ttcgtttcta acgattttct
    29701 aacgtttttc attataaaaa tttaagattg ggaaaaataa agtcattgtt gtcccttttc
    29761 cttcagttga ttcagccgtc aaatggatac ctaatttttc ggccagattt tttgctaaat
    29821 aaagtcctaa tcctgttgaa tgtgttttgc taagtcgacc atttctccca gtaaatccct
    29881 tatcaaagat ccgtttcaag tcttccgctg ggataccgat ccctgaatct ttaactgaaa
    29941 ggctgactcc ttgcgcttcc ttttctatgc tcacaatgat gtttccgtga tccggcgtat
    30001 atttgatggc attactaagc aattggttga agataaaagc gatccattta gcatcggtca
    30061 aaacagtatg atcctcacca acgatctcgt agcgaatatt tttttggata aaataatttg
    30121 cctgactgcg tatcacaggc tgaatgattc cttttagtcc atattcttgg atgaggtagt
    30181 cccgcgagaa actgtcgagt cttgcataat acaaaacttg ttctacgtat tcatcgattt
    30241 ttgataattc attttctagt aatgtatact tttgatcgtc aatatcgaac tcgatcgaac
    30301 gaactagcaa tttagaagcg gaaagtggca ctttgatttc atggacccat gagtcaatat
    30361 aatctttttg gtcttgttga ttactgattg ctgattgcat tacttcttgg tgttcacgga
    30421 tcagctgatt gatatattct tctaccagtt tttcttcttc tgtcctccca ccactcaaat
    30481 aattttgcaa aggggaagtt ttttgtatgt cggctaattt tccccaccat cttctcttgc
    30541 tcaagtaacc acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag
    30601 caattgtgct caaatcaact ttgatgtttg gcgccagcca catcacaaag atcgtcaatg
    30661 caataaaaaa cagccatccc aagatcaatg tccactgatc tttcaaatat ttaaaaaaat
    30721 tcatcttaac cgctcatcct ttcagggaac gatatatccc tgtcctactt ttgtttcgat
    30781 atatccttgg atacctgcct gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa
    30841 tgtattgtca tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt tttcccgact
    30901 taagatcttt ccattgtgct tcatcaaaat aaataataga cgatattcat ttttgctcaa
    30961 atcaatgatc tctccgttga tttccatact tccgttgtca acattcaacg taatgccatt
    31021 gtggctcatc atttcactag ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt
    31081 gatttttgca atcagtacat cgatttggaa atgttttgtc acaaaatcat ctgcccccat
    31141 gttcatagcc atgatcatat ccatattcgt attcctgctg gagataaaaa tgatcggtac
    31201 tttcgatact tcacgtattt tttgacacca ataatagcca tccaaaacgg gaagattaat
    31261 atctagtaat acaagctgtg gttcttctct ttcaaagtct gagaacactt gattgaaatc
    31321 ggtcgttcca aaggtttcaa attgccattt ctcaagttcc tccatgatca attgtcgaat
    31381 gatctcttcg tcttcgacta ccatgatttt tgccataaaa aatccctcca tctttcttct
    31441 tgctactaca atatcaaaga agcccttgtg aaacaagttg gcttacctgg aaaatgtcat
    31501 tttagcaaac gttaaaaaaa actggaaata tgcgatttaa tcgcgtttgt ctcttccaag
    31561 cagcttaaat ttattatgtt attcttaata attggtctat tatctgttat ttttctttcg
    31621 ttgtttttgt cttttaaatt ttcgataaca ccaataaccg aaaagcgtta gcactagcaa
    31681 tatacctaga aggagtataa gtaatcgata aagatggtat ttttttgtct gttctatctt
    31741 tgaagagact tcttctgtaa agggaactcg tcttcctgtc actagtaatc ggtgagtgtt
    31801 cacggtgtaa ggtgtacaag tgataagtgt gactagatcc tgcttttctc taattgctaa
    31861 tgtatttgtt tttgttggaa ggacaacaat tttctcaacg acttcataag cgagcatccg
    31921 accatacact tcaagataga atcggtcatc ttgctccatt tcttcaagat ccgtaaagag
    31981 ttttttctca gcaataccgc tatgggcggt aatcacggaa tgtgtactct ctcctccagt
    32041 tggaaaagag gtgccttgca ggactgtcgc tcctctatct aacaaaagat cattcgtttc
    32101 atcaaataaa ggcaagctaa cattgatttt cggaatatag atggctccga tcatatgttg
    32161 ttcgtaatat tcttttcctg gattttggac atttctaacc gcttgctcaa aaggatcttt
    32221 gacttgtccc attcctggga ttactgatgt atgagcaagc gtctgatttt tcttttcatg
    32281 cactgctaat tgttgctcat gtttttcttc tttatcgcgt tcaagctgct tttgataatt
    32341 ctcgatcctt ctttgatcca aataattgct gagagcatct gctaaaaatg gatatgtaaa
    32401 aactgcagct ccggcaagaa acatcactgc cataaataat tgtaataaaa gactgatttg
    32461 ggtttgaggt ctattaccta ttttcttttt cattttcttc accttcttat ttaaaaaaac
    32521 tgcagcaacc tttataaaac cgattgctgc agcttatttt tgttgcttat acttcagact
    32581 tcattttaga tttacggtac cagctgtacg ctccaatcat caaacttatt ccgattagta
    32641 agaatgccaa gagcccgttc ccaccagtag caggaagtga cccgcgtttc atattcggta
    32701 ctctttctgg ccctggattt tctactccta cgatctgaat ttcttcatta tagcttccat
    32761 aagagatgat aaattcagta aaggctgcct cacctgttgg aagtacataa ccttctggcg
    32821 ccatggtctc tttcatttga taggttccat aatctaaccc ttgaacctca aagtatccat
    32881 ttccatctgc gacaaattct gttgcttgtc cgcttgttgc ccactcaatt ctatctgctc
    32941 ctgattcata aacagctaaa ggcgactccc ctttataaaa tattgcgtat tcagaacggt
    33001 cttcattcca caattcgaat cttgcatctt taagcaattc actgcttgat gcatcatatt
    33061 tttcaaactt tcttccacct gttgtgactt tctctggcgg ctctactcct tcttcataat
    33121 caaattcatc atttcgtccg atttcaataa ccgctgtgtt gttatggaaa tcatccgctt
    33181 ctagttctgt ggaaaccttc aaattatacg tgatcgttaa atctcctcca cgtgcatctg
    33241 ctaatctacc taaagtatct actttggcta ctccattttc ttctgtcaaa atcgtcacga
    33301 caaacccgtt gcttactact tctactatat aatcttgtcc ttttgttaaa atcgtctcgc
    33361 cggctcgcac ttctatttcc tgatcataat aagttagacc aactgtaggc gtatctgtga
    33421 ttttgtaatt tttgataaca gcgcttccgt tttcaaggac agaatcgatg aaataaggaa
    33481 taggtgctgt gatcgtgtaa gaagccgttt ttcctctttc tagatcaata taagaaatcg
    33541 tttcttgttc tccagcttca ttaataatcg ttacttgtct aaggtcgctt tcctctacat
    33601 tcatctgttt atctgcttct ctgatttcgt ttttcggata caaatgcaca tctgtattgt
    33661 atgtgtcttt ccatgtagaa ccatcccaca cgccatccac catatcaggc atcatcactg
    33721 gcatcataac aaccattgga gacgcgattc tggaaatatg agctggcgaa taagtttcta
    33781 agaaaagata gactttatct ctagaagaag gtgcttcttc cgtcacttgg acggtagaga
    33841 ataccgcttc tccagcttca ttcgttgttt gtttgtcaat acttgtcagt cgatatttta
    33901 aaaaccaact ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca gaattaagac
    33961 cgtcttctcg ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat acttccgtta
    34021 cgtcaaacaa atcaaaatcg actcctggaa tattttcttc accaaaatcc gtagaaacca
    34081 atccgctatt cggtcgttct tccggaacag atgagaaacc ttttttgtgt aatgtaatcg
    34141 tcacttcact tggtttttgt gcgacttctt cggctttgac tagttttccg ttcccgaaaa
    34201 tccctattcc cgcagtaacg agtaacagta cactcattcc cgtgacaatc atttttttat
    34261 tcattttttt ctccccgttc tttttttatt aataaaacag cacctaacaa taaaagaatt
    34321 cctagtaaag aaatcaacgt tcttgcttca ttcgtttgcg ggaatacgga ccattttcct
    34381 gattgacctg gattgcttgg accactcggc tctttcggtt tgtccgttgt ttcttttacg
    34441 gtctcgttat aaacacgtgg tgttgcggat tcataggcag agactggaac tttcccggat
    34501 tctaattcag ccatctcctg tccgttaact actacatatt gtggttggcc ttttgcatcc
    34561 acccaaaatt cagggatgat gacctcgatt ctcttacttg cttcatccat ctcgtaacct
    34621 tccacacctt ttaattcttc aaaataatag gtgccagaag gcaagaaacg ttgtcctgtc
    34681 gtaacaaggc cctctttatc cgaaatgaat ttgcttacat ttttgtcatt caaaggatca
    34741 ttgtctgctg gtttgatcca ttgatttttc aagtcattag caggagccat atctaaataa
    34801 tatttccccc cttctatcat ctgatataga gcaaaaacag caccttctaa tggttttcct
    34861 ttttcagttg ttcctctttg acgtccatac ttgaagaagt atggatctct tagataaccg
    34921 acatttttag gatagatatg aatctcttgc agttcctcgg ttgattctgt cgggtgataa
    34981 attggcaaaa tagcagcaat tgggatagct gtctgatcga tatcaacatc caatccaact
    35041 tctggatcaa tcgattcttc gaaaatcaaa taaactgcat cccgatcttg gtttttctta
    35101 ggtgctttga tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt cccagttgtt
    35161 accgaagtcc ctatttggac aagcctttct tctgtgatta gctgacggat attgtttctg
    35221 ttcgtttcag cgatttgctg gacgaattct ttaggagtca tgttttcagc agcttttttt
    35281 tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag tggcaccgtc
    35341 tgtgaaatga tatctgctgt ttcccctgct tcctcagaga tagccaagcc atcattttca
    35401 tggtattcaa attgatcgtt ataatcgata tctcgaatca tccgtttatg caacacgaat
    35461 tccacgacat cttcccctga ttcttctgcc gacatcgcat gtggaagaag gactgctgca
    35521 agcggtagta aaacaagtaa ttgaagcatc aaacacaaca gctttttcat taccttgctc
    35581 cctccttttt agattgtctg acaaacaaac cataggctgc tcctgcaagc gccatgaaga
    35641 atgcagcaag caggatatag aataatcgtc cggatcctcc tgtagcaggc aacggatttt
    35701 caggatcgtt cccgacacta agctgtaatc tgattcgatt tgtctccaaa agttcatatt
    35761 ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt tggataatac gaaatctcaa
    35821 cttttagtgt acccgcttct tttcctggaa ctacccgatc attttctatg tcaggttctt
    35881 ctcgataact aatttctaaa ataaagtaac ctggaagttt tctatagctg tcaggtgact
    35941 tcacttcctt gatccgatag accccttcca acaagtctga agcattgaat agggaagaat
    36001 cgtttggatc tggtgccaag cctccctcga tgatttcata ctctcctgca ctattttgtt
    36061 tctcaattga aaattctgcg ccttcaagac gtcggctatt catactgtca attttcaaaa
    36121 gttccaaatc gattggcgct agttcatctt ctaaggttac ttgtagtaca ccatcgatga
    36181 tttcatagcc ttccggcaag ggagcagttt cgtcttcgat gaccgtatct tcaatgataa
    36241 acttaccttc ttcattcagt gcaaactgga acacttgatc agatagccga tacccatttg
    36301 gtgcgacaga ttccgagacc tcatattcac caatagacag atgcagaggt tggtcgtaat
    36361 ttaaatcgac atacttttct actcctacgg cacgagaata gaagtatccg atcccaggca
    36421 aacgtttcga ttcttctggt tctaacggat taccagtgta tgctcgatac agcgggaagg
    36481 aagaatcccc cactttcctg atatcgaaac ctgcaccatc taatgtatgg ccttcatggt
    36541 ctaattttct aactcggatc tcccgataat caaacgtatt gtaagcagtg aatgctaccc
    36601 ataaacgatc ctcttcatct gttgtatacg tgagatattg ccaattttct tcacctgttt
    36661 gcttgagttg gatagtccct atagcattat cgtaaagtcg caagatccat gtgtcatttt
    36721 catcaggttc tttgaaacct tctgggttct ggctctcaat gattttgtat tctgcatagt
    36781 tggtagtttc ttcactatcc ttataaacaa atgaatctgg ctctccgatt ggatgaataa
    36841 ctttctcacc atccacgatt acatatttcc cgtcttcatc tactgaatag aacgtgagtc
    36901 tccctttctc ttcctctact tcgttcacac cagaaagccc tttcgctact ggatatccga
    36961 tttccagtcc gttaccatct gtttcataaa gttcaaactc tgcgccattg attggcatat
    37021 tactgccttc aattagttta ttgatattca tgtggattgg cttgaattca tttcgcattt
    37081 ccagtttgac atacaactca tcatactctg tgccttctat gttaggctct tgtacaacag
    37141 aaagcttgtg atattctcct tcataatagg aattcggatc tttaccttcc ggtccgccga
    37201 ctctcgaacc agatgtatat tcatctacaa gctcgtagtg acctaacatc tcatcttcgt
    37261 tggctgcata ttttttgatt tggaattgat acgtctcatc tactaactga taacgtggtg
    37321 gtccagcgat ctctctgatc caaaatatgt cgctgactgc tccagtttca tctcctaaaa
    37381 tcggcatctt cgtttcaaag atcgctattc cgtccgtggt agttttactt gtttcgattg
    37441 gttcggcttc cccactctct ggattttggt catataatgc aaattcgact ccagccaacg
    37501 gctcgccatt ttgacccttc ttgctgactt ggaaattacg gcctttatgt tcgtaacgaa
    37561 tggaagaaga tgggaaatac atccgtccag tagtgatatc attctcataa tcctccaggt
    37621 tctgaatata ggccatattg ttcactgtgc aaaaatttcc gtcttgagct cccgcatcaa
    37681 gttttacttg gtacttgatc ctgaccattt ccccttcacc taaagaaatc ggagaaaatt
    37741 tataagcttg gttttcccgt ccgacatacg ttgtcggagt cgccactact tctggtgggg
    37801 cttgtccatg acgttcttca tcccatattt ctggctcttg gtacttcatg ccgttttctc
    37861 ctggatctag atggtaaaac tgaatatctg ccgatctgcc agctccaaat ggtgagacat
    37921 taaaaccagt ttcatcataa agtgtggctt gctggatcgt tctcttgaag gtagaagaga
    37981 tatcctccaa ggctgtaaca atgtcattct tgttgttcgc attgtaatat cgcggtgtta
    38041 cgccatcttc ttctaaatca gcaatatttt tcaatacatt tcgtccaata gcatctagtc
    38101 gttgtctccc gctaacgttt ccatcgattc ctaaaccaat ggagaagata cttggcgggt
    38161 tctcttgata cttgtttttc agccaatgat gataagcaac tgtgttgatc gcagatgact
    38221 tttcttgcga agtaggactg ccattcgtat tgttattaga agaaccatca ccaacccagt
    38281 gagtcgcttt attatctcct tcttttacct ctccataacg atagtgtaca tttacttcat
    38341 cttctggacg gttaaagtca gatgcatagg taactggatg agtaaaccct gcattagaag
    38401 tattcccaga aaactcctca aaatttctga acaaaacgtc attatcttca gcaatcttat
    38461 cttccatcac agtccagttg ctccaagctc cattagtgct cgtacgataa cttgactgaa
    38521 tcggtgcata actgaaggtc ggtgtcccat ctcccacaac gattaggatt ttttctggat
    38581 ttctattttc tccgccattg tctttataca atgtttcata gccattttta agccctagtg
    38641 taagcggagt tcctccgatt ggtgtacgat ttaatgctgt attctcactt cccgtcaaac
    38701 gattgatatc atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg ttattgatag
    38761 ctgtactatt atggtaaaaa ttcaccatac cgattcgaat atccagactc gggtcagaga
    38821 gcagatcttt actcatttcg tttactgcct caatcaacgc agcattttta gtttgactat
    38881 tggtcccagc ggttaattca ctcatactcg ctgacttatc tagaacaaaa acgatatcga
    38941 ttggactgat ttcctttttc tcgcccccga tgacatctag atagacattg taaagggttg
    39001 ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg cagaacaggt tctacccatt
    39061 ttttgatgta cgccccgtta taatccaagt aactgttttc aaaattccgt tcatttcccc
    39121 aaagctgaac ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt gatccattat
    39181 tagtagcata gtcgtaattt cgccagttcg ctcccccgtt tgtataatca ctatcatcga
    39241 tcaagttctc tccgtcatat ggcgagcctt tgtctccttg atagcgttca ttgctttgtg
    39301 tccaatttcc taacactttt gtatagtcat tcgttgtaaa cttcgggtag cgatgctgtt
    39361 cgttttcaac ttctccgcta ggatagatat attcataggg gtcttgcgta gctgttggcc
    39421 caagacgctc caaaataccg ttgaataact ggcgttgaat gcgctggagt gcttgaccgc
    39481 tgggtacgat tggttcgtcc agcttttcca tctcattttc ttcgctacgt tgaatttcag
    39541 cggaatcttc tgtatcagtt tcattttctt gtaattcttg gttatctatt ctttcatcaa
    39601 ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa
    39661 tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat
    39721 cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac
    39781 gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac
    39841 cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt
    39901 caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg
    39961 tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg
    40021 gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat
    40081 ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca
    40141 tctaattata atttaattta attttaattt taaatcaaat gatatc
//
LOCUS       DASJSZ010000016        39455 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.016, whole genome shotgun sequence.
ACCESSION   DASJSZ010000016 DASJSZ010000000
VERSION     DASJSZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.016"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..861
                     /locus_tag="U4B03_000801"
     CDS             4..861
                     /locus_tag="U4B03_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEN2067673.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            976..1896
                     /gene="pstC"
                     /locus_tag="U4B03_000802"
     CDS             976..1896
                     /gene="pstC"
                     /locus_tag="U4B03_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEN2067674.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1896..2780
                     /gene="pstA"
                     /locus_tag="U4B03_000803"
     CDS             1896..2780
                     /gene="pstA"
                     /locus_tag="U4B03_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEN2067675.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2795..3601
                     /gene="pstB"
                     /locus_tag="U4B03_000804"
     CDS             2795..3601
                     /gene="pstB"
                     /locus_tag="U4B03_000804"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN2067676.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3617..4375
                     /locus_tag="U4B03_000805"
     CDS             3617..4375
                     /locus_tag="U4B03_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN2067677.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4388..5065
                     /gene="phoU"
                     /locus_tag="U4B03_000806"
     CDS             4388..5065
                     /gene="phoU"
                     /locus_tag="U4B03_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEN2067678.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5303..6334
                     /locus_tag="U4B03_000807"
     CDS             5303..6334
                     /locus_tag="U4B03_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN2067679.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6331..7098
                     /locus_tag="U4B03_000808"
     CDS             6331..7098
                     /locus_tag="U4B03_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN2067680.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7198..8766
                     /gene="liaX"
                     /locus_tag="U4B03_000809"
     CDS             7198..8766
                     /gene="liaX"
                     /locus_tag="U4B03_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEN2067681.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9063..9383
                     /locus_tag="U4B03_000810"
     CDS             9063..9383
                     /locus_tag="U4B03_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEN2067682.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9383..9733
                     /locus_tag="U4B03_000811"
     CDS             9383..9733
                     /locus_tag="U4B03_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN2067683.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9900..10838
                     /locus_tag="U4B03_000812"
     CDS             9900..10838
                     /locus_tag="U4B03_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEN2067684.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10853..11683
                     /locus_tag="U4B03_000813"
     CDS             10853..11683
                     /locus_tag="U4B03_000813"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN2067685.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11727..12752
                     /locus_tag="U4B03_000814"
     CDS             11727..12752
                     /locus_tag="U4B03_000814"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN2067686.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12767..13705
                     /gene="galU"
                     /locus_tag="U4B03_000815"
     CDS             12767..13705
                     /gene="galU"
                     /locus_tag="U4B03_000815"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN2067687.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13830..14270
                     /locus_tag="U4B03_000816"
     CDS             13830..14270
                     /locus_tag="U4B03_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEN2067688.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14273..14875
                     /locus_tag="U4B03_000817"
     CDS             14273..14875
                     /locus_tag="U4B03_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN2067689.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14934..16040)
                     /locus_tag="U4B03_000818"
     CDS             complement(14934..16040)
                     /locus_tag="U4B03_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN2067690.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16250..17251
                     /gene="ccpA"
                     /locus_tag="U4B03_000819"
     CDS             16250..17251
                     /gene="ccpA"
                     /locus_tag="U4B03_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEN2067691.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17588..19933)
                     /locus_tag="U4B03_000820"
     CDS             complement(17588..19933)
                     /locus_tag="U4B03_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN2067692.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20212..21114
                     /locus_tag="U4B03_000821"
     CDS             20212..21114
                     /locus_tag="U4B03_000821"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEN2067693.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21327..23102
                     /locus_tag="U4B03_000822"
     CDS             21327..23102
                     /locus_tag="U4B03_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEN2067694.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23168..24754)
                     /locus_tag="U4B03_000823"
     CDS             complement(23168..24754)
                     /locus_tag="U4B03_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEN2067695.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            25005..25229
                     /locus_tag="U4B03_000824"
     CDS             25005..25229
                     /locus_tag="U4B03_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEN2067696.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25370..27121
                     /locus_tag="U4B03_000825"
     CDS             25370..27121
                     /locus_tag="U4B03_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2067697.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27121..28905
                     /locus_tag="U4B03_000826"
     CDS             27121..28905
                     /locus_tag="U4B03_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2067698.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29231..30082
                     /locus_tag="U4B03_000827"
     CDS             29231..30082
                     /locus_tag="U4B03_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2067699.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30178..32058
                     /locus_tag="U4B03_000828"
     CDS             30178..32058
                     /locus_tag="U4B03_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2067700.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(32098..33000)
                     /locus_tag="U4B03_000829"
     CDS             complement(32098..33000)
                     /locus_tag="U4B03_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2067701.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            33231..34634
                     /locus_tag="U4B03_000830"
     CDS             33231..34634
                     /locus_tag="U4B03_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2067702.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            34634..36067
                     /locus_tag="U4B03_000831"
     CDS             34634..36067
                     /locus_tag="U4B03_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2067703.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            36212..37138
                     /gene="trxB"
                     /locus_tag="U4B03_000832"
     CDS             36212..37138
                     /gene="trxB"
                     /locus_tag="U4B03_000832"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEN2067704.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            37352..39079
                     /locus_tag="U4B03_000833"
     CDS             37352..39079
                     /locus_tag="U4B03_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEN2067705.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            39094..39444
                     /locus_tag="U4B03_000834"
     CDS             39094..39444
                     /locus_tag="U4B03_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN2067706.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT        12615 a         6968 c         8500 g        11372 t
ORIGIN      
        1 ctaatgaaaa aacttaaatt attcttacca ataataggat tagctattct tttatcaggc
       61 tgttcaaagt ggattgatcg aggagaatcc attacggctg ttggctcttc tgctttacag
      121 cctttagtag aaacagttgc tgaaacttac cagacagaaa atcctggaaa attcatcaat
      181 gtacaagggg gcggaagcgg aactggtttg tctcaagtac agtcaggagc agtcgacatc
      241 ggaaactctg atttgtttgc ggaagaaaaa tcggggatcg atgcctcagc attagtcgat
      301 catcgagtag cagttgttgg gatcactcct attgtgaaca aggatattgg cgtaaaagac
      361 atcagtatgg aaaacctgaa aaaactattt acaggcgaga tcaaaaactg gagtgaagtc
      421 ggtggaaaag atcagccagt agttatattg aaccgcgctt caggaagcgg aacccgtagc
      481 acgtttgaaa aatgggtatt ggacggagaa acagctgttc aggcacaaga acaagattca
      541 agcggaatgg tgcgccagat cgttgccgac acacccggtg caatcagcta tgttgctttt
      601 tcttatgttt ccgatgatgt agatacgttg aatgttaacg gtgtagaacc gacagatgaa
      661 aatgtgacaa cgaacaaatg gacgatttgg gcatatgagc atatgtacac aagagatcat
      721 ccaaaaaaac tgacaaaaga atttttagac tatatcttat ctgatgaagt acaagataac
      781 atcgttggag aactaggtta tatccctgtt tctcatatga aagtcgaacg tgattggcaa
      841 ggaaatatcg ttaaagagta atctttacac aaaatttaca aacttagcta aacttttaca
      901 tgttattaaa accaaattga tgttcaattt gtacactgag tgagtatgtt aaacctgatt
      961 aagggggagt catctgtgga agatgtgaaa aaagtattat taacaaaatc aaaacgttca
     1021 aaaatggaac agcgtggaaa attcatcagt tttttatgta tcgcgctgat cgtgttggtt
     1081 gtattatcta ttttttattt tgtagctagt aaaggattgg ccaccttttt tactgataag
     1141 atcaatgtat ttgattttct gtttggtaca gattggaatc caagccaaat cggagcaaat
     1201 gggaaaccaa tggtaggggc attgcctatg atcacaggtt cgtttattgt gaccttttta
     1261 tctgctatcg ttgccacgcc atttgccatt ggtgcagcag tattcatgac ggagatctct
     1321 cctaaaaacg gaacgaagat cttacagccg gtcatcgagc tgcttgtagg tattccttct
     1381 gtcgtttatg gttttatcgg tttatcagtt atcgtgcctt ttgtcagaag tatttttggc
     1441 gggactgggt ttggtatctt agctggtaca tttgtgttgt tcgttatgat cttgccgact
     1501 atcacaacga tgactgttga tgcattaaag gcagtgccac ggcactatcg ggaagcttca
     1561 ttagcgcttg gtgccacaag atggcaaaca atctacaaag tcgtgctacg agcagccgtg
     1621 cccgggattt taacagcagt cgtttttggt atggctcgtg cgtttggtga agccttagct
     1681 atccaaatgg tcatcggtaa tgcagcattg atgccaacag gtcttactac tcctgcttct
     1741 acattgacgt ccatcttaac aatgggaatc ggaaatacaa tcatgggaac agttgaaaat
     1801 aatgtgttat ggtcattagc gctgatcttg ctattgatgt cattattctt taatatcgtg
     1861 atccgtatga ttgggaagaa aggagagctg aaataatgaa cgcaaaacgc tcagataaaa
     1921 ttgctacagg tgttttatat atagtttcgg ggatcatcgt attgatttta gcagctttac
     1981 tactttatat cttagttcgg ggaataccgc acatttcatg gtccttcttg accgaacctt
     2041 ccaaagccta ccaagaaggc ggtggaatcg ggatccagct atttaactcg atttatttgc
     2101 ttttgatcac gatgatcatc agttttccta tttctttagg tgcgggtatc tatctttcag
     2161 aatatgctgg aaaaaactgg ctaacagatg tgattcgcac gtcgattgaa atcttgagct
     2221 cgttgccttc tgtcgttgtc ggtttattcg gtttcttggt tttcgttatt caattccagt
     2281 atggtttctc gattatttct ggtgcattgg ctttgactgt attcaacttg cctcttttga
     2341 ctagaacagt tgaggagtct ttacaagcgg tccattacac acaacgagaa gcaggattgg
     2401 ccttaggttt gtcacgctgg gaaacagtga taaaagtggt ggttccagaa gcgacaccag
     2461 ggattttgac tggtgtgatc ctaagttctg gaagaatttt tggtgaagca gctgcattga
     2521 tctatacggc tggacaaagt gcacctgcgt tagattttac gaactggaat ccattaagtg
     2581 tttcaagccc gatcagcatt ttccgtcaag ctgaaacatt agctgtccat atttggaaga
     2641 tcaatacaga aggcacgatg cctgatggtg tttctgtttc ggctggcgct tctgccgtgt
     2701 tgattattgc tgttttactg ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc
     2761 gaaaaatgac ttcagcctaa ggagaagaag caaaatgaaa aaatataact ggaatgaaac
     2821 aaatgtcatc accatgccaa aagagaaaga agtagcacta tatacggatg atctacatgt
     2881 ctggtatggc gacaatgaag caatcaaagg agtggatctg gaatttgaaa aaaataagat
     2941 cactgcactg attggtcctt ccggctgcgg aaaatctacg tatcttcgtt cgttgaatcg
     3001 aatgaatgat gggatcgcta atacgaaaat cactgggaaa atcatgtatc aaggcgtaga
     3061 tattaatgcg cctcaagtgg atgtttatga gatgcgcaaa cggattggca tggtttttca
     3121 acgtcctaat ccgttcagca agtcgattta tgacaatatt acatttgctt tgaaacagca
     3181 cggcgaaaaa aacaaaaaga aattagatga aatcgttgaa acaagcttga aacaagctgc
     3241 cttgtgggac caagtaaaag acagcttgga caaaagtgcc ttggctttat ctggcgggca
     3301 acagcaacgt ttatgtattg ctcgtgcgat cgcgatgaaa cctgatatct tgctgctaga
     3361 cgagccggca agtgctttgg accctatttc gacaggaacg gttgaagaaa cacttgtaaa
     3421 cttgaaagaa gactatacga tcatcatcgt gacgcataac atgcaacaag cagctcgtat
     3481 cagcgactat actgcctttt tctatttagg aaaagtaatg gaatatgaca aaactaacaa
     3541 gatatttacc agaccaaaaa ttcaggcaac cgaagattat gtgtccggtc attttggtta
     3601 agacggaggc ctcaacatga caaaagagat tatttcatct aaagatcttc atctgtatta
     3661 tggaaagaaa gaagcactaa aagggatcga tctaagcttc aatcaagggg aaatcactgc
     3721 gatgatcggt ccttctggtt gcggaaaatc cacttatctg cgttctttga accggatgaa
     3781 tgacctgatt ccgaatgtaa cgatcaccgg aagtgtttta tataaagaca aagacatcta
     3841 tggtccaaaa acagatgttg ttgaactaag aaaagaaatc ggcatggtat tccagcagcc
     3901 aaacccattt cctttttcta tctatgaaaa tgtcatctat ggattgaaat taaaagggga
     3961 aaaagataaa aaagtccttg atcaagtagt tgaagaaagc ctgaaggcag cgtctgtttg
     4021 ggatgatgtg aaagacaaat tgcataaaag tgctttatct ttatccggtg ggcagcagca
     4081 gcgtgtatgt atcgcacgtg ttttggcagt taatccggaa atcatcttat tggatgaacc
     4141 aacaagtgcg cttgatcctg tttctactgg gaaaatcgaa agtatgctgt tagaattaag
     4201 agagcggtat acgatgatca tcgtgaccca taatatgtct caagcttcac gtatttcaga
     4261 taagacggca ttctttttag atggtcactt gatcgaatac aatgatacga agaagatatt
     4321 catgaatcca gaaaaacaag agacagaaga ttatatttca ggtcgatttg gataaggagg
     4381 aaacggaatg ctacgtacac aatttgaaga agaattgctg aatctccata atcagttcta
     4441 cgaaatgggg atgatggtga gcagcgccgt gcacaagtca gttcgtgcat acatcaaaca
     4501 cgataaaatc ttagcccaag aagttatcga aaacgatata aatatcaata acatggaaac
     4561 acgcttggag aagaaaagtt ttgaaatgat cgcattgcaa cagccagtta caactgattt
     4621 gcgtatgatc attacggtca tgaaagctag ctcggattta gaacgtatgg ccgatcatgc
     4681 ggtgtctgtt gctaaatcca cgattcgcgt aaaagggcag acgagaatac cggaaatcga
     4741 aaaagagatc tccgatatgt ccgattatgt gaaaaaaatg gtggacaacg tcctagttgc
     4801 ttatgtcaaa acagacgaaa aagacgcacg tacgatcgcc aatatggatc aaagagtcaa
     4861 tgactatttc aatcgaatct ataatgcaac aatcaaaaat atgcaggaaa atcctgagac
     4921 agtcatcagt ggaacagact acttgaatgt tgccagttat ctagaacgga tcggagacta
     4981 tgtgacaaac atctgtgaat ggatcgtcta cttggctaca gggaaaatca ctgagttaaa
     5041 tacgaaccac aatgagattt tctaaactga agctgtgatt atgccgtttg tttaggaaaa
     5101 gaggttgtga aatcagcggt tgtgaaaaag ctgttctgca tcaagtaata agaacagtca
     5161 aacaaaaaat agctcctcat atttttcgtt tggctggact tattaccgca gatgcaagct
     5221 tttgaacacc gtttattcgg gaaagggttt gtgacaaaac atgtcacaaa cccttttcat
     5281 catttcatag aaaggataaa gaatgcaaaa aaaaattagc atcatcacta tattttttat
     5341 ttgtgtgctt acttcatggg ttattcagca tcaagccgct ttatatgaag tacctgtcat
     5401 cgagatagaa aaagtacgaa taaaagagga tcatcaattg attacaggaa aactgatgaa
     5461 cacggatcaa aaaggagaat cgatttattt atcggttcct tatcaaaaaa atcaaataga
     5521 aacagcaaat ataaaagcga gacaaatgta tctagtccaa atgcatggag atgaatggca
     5581 gctagtagca aaaaaaaatg atggctggct tttcttcttc acaagtatct tcattggcgc
     5641 catgttgctt attggtggaa aacagggtct atttgcttta ggaagtctca tcatcaatat
     5701 cgttttactt ttacttttgt tgctcatctt tactaaaaca gaagggattt ctctattaaa
     5761 tatatctatc ctgttcgtcg ttatagggat gattattagc atattggctt tagatggatg
     5821 gaatagaagc agtataatca aaatttcagc tgcagttacg agtgtttttt tagcattttt
     5881 tatttgtctt ttgacaatga atcattggaa agaccaagga ctgcgctatg aggaactgaa
     5941 ttatctgaca cgaccgtatc gttctgtttt tctttctagc gtactgattg gaacagctgg
     6001 aggaacactc gataccgtta tcacagtcat tgcgacatta gaagaattga ctaagcgaca
     6061 accagaaata acagcgaaac agttatggct ttcaggaagg caagtgggtc gagatgtgat
     6121 tggctcgatg accaatatcc ttttgttcgt ctatatcagt gggtcagttc catcgcttct
     6181 tttatattta ggaaatcaat ggtcgttcaa agaaacgata gaacagcatt tatcattgga
     6241 atttttacgc gtcattgctg gcagcttagg aatagtatta tcgatcccta tctcggtcct
     6301 cttttttctg actgtaagga ggctgaaacg atgaatgtac ttattatact tgttgggatt
     6361 ttagcaatca gcgtgctact tattggcggt acgcaaggta tgtatattct tttaggctta
     6421 ttcatcaatt tagggatctt tttcctgttg ttattcggtt atcatcagaa gtggccgata
     6481 ctggcattgt cggtcatagg atttttattg attgctgtgg ttatcttatt ttttatcaac
     6541 ggttataatc taaaaatgcg tgcagctttt gtgagtatac tgatcttttt attttgtttc
     6601 ctactattgc ttcctatcac tgatttttta gcaatccaag gttttacatc ggtagaacta
     6661 gaagaattat ctggcttaga taagacttta gcgattgatt ttagattact tacgagatcg
     6721 ttgcttctga ttagtcttag tggtgctgtt ttagatgcga gtgtagcaat ttctagtggg
     6781 acattcgaag tatatcaggc aaatccgcaa ttatcttttt accaattgcg ccattctagt
     6841 ttcgctattg ctaaaaaaat attggcgtca acagtaatga cacttctttt tgcattcatg
     6901 ggaagttctt tagcgttgat cctctggttt attgatttag acattccttt tcagcaagtg
     6961 atcaatgaaa aatcatttgt tctagaatat acgatggcta ttcttaccac attgtccgca
     7021 ctacttgttc ttcctttaac ctgcgtgact acagcatatc tctttacaaa aaagaaagaa
     7081 caactcaaga tggagtaaaa gttacaactt aagtcctatg acatttttac tgaatcagtg
     7141 cataatatgg atgtaaggaa aagcttgcaa acaattttgc ttaaggaggc aatttccatg
     7201 aaagaaagag aacgtatttt agatttagtg aaaaaaggaa tcctttcgac tgaagaaggc
     7261 ttagatttat tagaaagcat ggcaactgaa aaagacgaaa agcaaatcaa aaaagaagca
     7321 gacaaggtaa ctgctagtca taaagaaaaa gatcaggcaa gtcaattgat cgataaactt
     7381 gaaaacggcg aagaagaaat ttctgagcca gttgatccaa aggaaagaga aagacaagat
     7441 caagaaaact tagaaaagat cttagatgaa ctagcaactg aagcgaataa aacttcggcg
     7501 cgtctagatg aagtaaatgc tcaattcgct gacaataagg cagcacgcaa tgagaaacag
     7561 gaagcattga tgcagctgaa tacaaaagaa gaattaggcg agttgacaga agaagaatta
     7621 gcacagcgtc agacgttaga agcagaaatc aaagaattag aagctagtgg cgatacactg
     7681 ttagaagaac aattgaaatt agaagcagaa ttaaaagata tccgtaagaa tcaatggtct
     7741 gagaaaaaag aaacgtttac tgataagttt gaattgccag atgattggaa agatcaagca
     7801 acagatacac tgaatcaagt aggcgaaaaa atgtcagaag ctggatcaca gttaggcaaa
     7861 ttcttgaaga aaacattcca aactgtttca gaaacagtga atgacaacat ggaatggaaa
     7921 gatgtcagct tacgtgtccc tgggattgcg acaacgaaat ttgagcatga attttactat
     7981 gaagctcctg cagcaagtat ccttgatatc aaggcagcaa acggaaacgt gacgttaaaa
     8041 acgtgggaca gcaacgatgt gaaagttgaa gctaagatca aactttacgg aaaaatgggt
     8101 gctgaaccat ttgaagcatt ctctgaacgg agccagattg aggttaatga agatcatatc
     8161 tccttccaaa ttccaaataa acgtgtgcgt gcagaccttg ttttctactt acctaaacga
     8221 gtatacgacc acgcagctat taaacttcta aatggcaaca tcatgattga aacattggaa
     8281 gctaaagata tttatacaaa atctacgaat ggtaacatca tcgtggacca attaactgca
     8341 acgatgctgg aagtagaagg tgtaaacggc aatatcgata ttcgaaacgg gaacatctta
     8401 gattctatta tcgaaacagt aaatggtaca gtgacatttg gtgcgacagc agaaaacctt
     8461 tctgtgtctt tggtaaatgg tgatgtacgt cttacgatta aagaagataa tttgaaaaaa
     8521 gtagaagcaa gttcagtgaa cggaaatgtc aaagtcgctt taccagatgg catcggtttg
     8581 gaaggacatg ctaaaacaag tcttggaagt atcaacagcc gtctgtccaa ttacgaagta
     8641 gttcgtgaga aaaaagaacg gacaaaccaa atgttgcaat tccgtcgtgt ttcagatggt
     8701 gaaatcgcgc aagtgcaact ttcaacgaca acaggaagta tctatttaaa agatacagat
     8761 aaataaagag gttgtgaaag agatgttcag ccttgagcaa taagaagcga ttttgtaaaa
     8821 tagcttcccc tattttacaa aattgtgcct tattgccgat agtctatctc ttgaacaccg
     8881 tttaccaaga gattatgaac ctgccaatta tcgccgagtc acaaggaata atttcagaaa
     8941 aagctcgtca cgttttatga atatggtaat ttgtgaccga gaattttgca gaaagacacc
     9001 gattaagaag aaggttgtca caacgatttt cttcatttaa aatagtagaa cagaggtgag
     9061 acatgatgaa aaaaaagttg acaaaatcta ataaaaatgt tgttttgaca gggactttag
     9121 caggaatagg agaatacatc gggattgatc cgactgttat ccgtgtgctt tatgtattcc
     9181 tgagcttagt tgcttttgga agtccaatta ttttatacat tttattagct ttgattatcc
     9241 catttgcgcg ttctgcacga acaaactatg gacataacaa tgcatattat gaaaaaaaca
     9301 attatcgtga acaaactcgt gaacaaacaa aaaaacgcaa agaagcggaa aaaatcgatg
     9361 atgacgaatg gagtgacttt taatgtcgta ctttcaacgt attttagtta atacgttaac
     9421 attcatatca ttggctgttc tttttcctgc gatggtccat gtgagcagta tttggacggc
     9481 cgttcttgcc agcttcgtat tgtctgtgtt gaatatgttt gtcaaaccga ttttgacgat
     9541 actgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta tcaatgcagc
     9601 gattcttaaa atgacctcct tttttgtggg agcagaacat tttagttttt cttcattcgg
     9661 tgcagcgttg ttagtagcta tcttgatgtc gcttgtcaat gtaattgtca gcgaacataa
     9721 cagtagaaaa taaaagaaag ctgtgccgaa ctatcggcgc agttttttta tttagaatca
     9781 ggaatgcgtt ttctattaca ttggtaatta aataaaaacg ttttttttaa ttggtagtac
     9841 ttttggctag acaatgataa aatgatagag aacactctaa tagaaatgag gtagcatgaa
     9901 tgtcagaagt tgtaaaagtt agccaattag tgaaaaaatt atctttggaa atcgtgacag
     9961 gcgacgagaa aagcttagac cgtgtcatcg taactgggga catctcacgc ccaggactag
    10021 agttgactgg ttattttaat tattattccc ataatcgttt acagctattt ggcagtaaag
    10081 aaatcagttt tgcggaacgg atgatcccag aagagcgttt gatcatcatg agaagattat
    10141 gtagtgaaga tatgcccgca tttattattt caagaggatt accagctcca gaagaaatga
    10201 tccaggcagc aaataaaaat gggatagctg tactgcgttc tcccatctca acttctcgtt
    10261 tattaggaga attatccagt tatttagatg gtcgccttgc ccctcgaaca agtgtccacg
    10321 gtgttttagt ggatgtttac ggattaggtg tactgattca aggtgacagt gggataggaa
    10381 aaagtgaaac agcgttggag ttgatcaaac gcggccatcg attgatcgca gacgatcgag
    10441 tagatgttta tcagcaagat gaattaacgg tgatcggaga accgcctaaa atcctggaac
    10501 atctgattga aatccgagga atcgggatca ttgatgtaat gaatcttttt ggtgcgagtg
    10561 cggtacgtgg ctctatgcaa gtccagttgg ctgtttatct ggaagcatgg gcaaaagata
    10621 agaaatacga ccgtctgggc tctgaagaca cgaccgttga aatcgcggaa gtgggcattc
    10681 cacaaataaa aatccctgtc aaaacaggac gaaacgttgc aatcatcatt gaagtagccg
    10741 cgatgaattt ccgtgcacgt acaatgggct ttgacgcaac gaaaacattt gaagaaagat
    10801 taagtcgctt gattgaagaa aactcaggca acgactagca aggagtgaca atatgttagc
    10861 acaaatcaat cgagttgctt ttgagcttgt agggattcct atctactggt atgcattgat
    10921 catcgtatca ggtatcatta tcgctatgtg gcttagcagc agagaggctg ttcgggtagg
    10981 attaaaagca gatgatgtga cagattttat gttgtgggga ttgcctatct ccattattgg
    11041 cgctcgctta tattatgttt tatttgatct tccccagtat atagctgatc caatccaaat
    11101 attcaatatc cgttcaggag gcttagctat ttatggcgga ctgattgcag gcgcgatcac
    11161 gctgtatttt tttactaaat atcattttat ttctttgtgg acttttttag atatcgctgc
    11221 accaagtgta ttattagcac aggcaatcgg tcgttgggga aatttcatga accacgaagc
    11281 atatggacca gacacgacgc gaagcttctt agaaagtctg catttaccgc aattcatcat
    11341 tgataatatg tatatcgaag ggatctatcg tcaaccaaca tttctttatg aatcactttg
    11401 gagtttgatt ggatttattt tgcttttgtt tttacgaaag aaaccacacc ttttgaaaaa
    11461 aggcgaagtc cttttatgtt atctaatgtg gtatagtttc gggcgtttct ttattgaagg
    11521 attacgtaca gacagtcttt actttttagg tgtgatccgt atttctcaat tattatctgc
    11581 tgtgttattt gtcggtacat tgattctatt tatttggcgc agaaaaaaag gaaatttacc
    11641 ggattatgac cgctcattag gaaaaaatca acaaattatc tgatttttag agcgttaaaa
    11701 atgaaaaaaa tttcaggagt ggtaaaatga attatagagt agcggtttta ggaccaggct
    11761 cttggggtac tgctttggca caagtcctcg ccgaaaacgg acatgacgtt cggatttggg
    11821 gacatcgtgc ttcacaagta gaagaaatca acacccaaca cacgaatcaa cgttatttac
    11881 cagatcatta tctacccgat tccatccaag cattcaccaa tatggaagat gcagtaaaag
    11941 acgcagatgc ggtgctattc gtcattccaa cgaaagcaat acgttctgtt gcaaaagaac
    12001 tagtgcctaa attgaataca aagccagtga tcatccatgc aagtaaagga ttagaacagg
    12061 gaacacataa aaggatctct gacatcttgt tagaggaaat cccagcagaa aaaagaaaag
    12121 ctgttgttgt tctttctgga ccaagccatg cagaagaagt agcaaaacat gacatcacta
    12181 cgatcacagc tgcaagtaca gatgcaaaag cagcaaaata tgtacaagag ctgttcatga
    12241 ataattattt tcggatctat accaatcctg atgtgatcgg tgtagagatg ggggcagctt
    12301 tgaaaaatat tattgctatt ggtgctggcg ctattcacgg cttagggttt ggcgataatg
    12361 caaaggcagc aatcatgacg cgcggattag ctgagatcag ccgtttaggt gtagcaatgg
    12421 gtgccaaccc gctgaccttt atcggattaa gcggaatagg cgatctagtc gttacttgca
    12481 caagtgttca ttctaggaac tggcgcgcag gcaacttgtt aggacaaggg cagcccctag
    12541 aagaggtgct agccaatatg ggaatggtcg ttgaaggtgt gaatactacc caagcagcga
    12601 tggaactagc ccaaatgatg gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc
    12661 tgtatgaaca aaaagaaatc aaaacagccg caaaagaaat catgttacgt gatggaaaaa
    12721 aagaaaatga attctcatta acaatttttt gaaggagcga atgtatatga aagttaaaaa
    12781 agcagttatt ccagcagcag gattaggaac aagattttta ccagcaacaa aagctatggc
    12841 aaaggaaatg ttgccaattg ttgataaacc aacaatccag ttcatcgttg aagaagcgtt
    12901 gaaatcaggt atcgaagaca tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga
    12961 tcactttgat tcaaatattg aattagaaac aaacctaagt gaaaaaggaa agaccgaatt
    13021 attgaaatta gtagaagaaa cgacagatgt caacttgcat ttcatccgtc aatctcatcc
    13081 aaagggttta ggacatgccg ttcttcaagc aagagccttt attggaaatg aaccatttgt
    13141 cgttatgcta ggcgatgata taatggaaga cgaagtacca ttgacacagc aattaatcga
    13201 tgattacgaa cgtactcatg cttctacgat cgccgttatg caagttcctc atgatgaaac
    13261 ttcaaaatac ggaattatca atccaggaga agtattggaa gatgggttat acaatgtcaa
    13321 aaactttgtt gaaaaaccag atccaagcaa agcaccaagt gatttagcta ttattggacg
    13381 ttacttattg actccagaaa tttttgatgt attgtccgag caacaaccag gagccggaaa
    13441 tgaaatccaa ctaactgacg caatcgatac attgaataaa acacaacgcg tatttgcccg
    13501 taaattcaca ggtaaacgtt acgatgtcgg tgacaaattt ggttttatga aaacaagtat
    13561 tgaatatggt ctaacacatc cagaagtagg tgcaccttta cgtgagtaca tcctgtcttt
    13621 aggtaaaaca ttagaagcag aaggaagcaa gatcgatcat aatccaaaag gaataacaga
    13681 aatcaaagaa gaaaagaaaa aataaccctt ttttctaacg agcttcagct gctaattgaa
    13741 taaaaagtag ttgaagctcg tttttatttg tttaaaaaac acgaaaatgc tatccttata
    13801 gaaaagagag acaagaaggg agtttgatca tgtcaggagg agaaattgca ggattgatcg
    13861 cagcaatcgc ctttgcgata cttgtcgttt ttatcgttcg tgtgttgttg caagtttcta
    13921 aaactttaga aaaagtagat cagactgtag cagaagcaaa tacgacgatc gaaatcgtca
    13981 caaaagacgt agatcttttg tctagacaag tagaaggctt actggtcaaa agtaatgaac
    14041 tgctcttgga tatcaatgga aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg
    14101 atttaagtga aagtgtatca gaacttaatc attctagtaa aaacatcgcg acgaaagtcg
    14161 gcggaatcgg taaaacgaca gctaaagcga cggttgccgg aaaagtcggt gggacagcga
    14221 tgaaattttt taaaaacaaa aaacatactg aaacgacagg aggaaaataa aaatggctaa
    14281 aaaaggtgga tttttattag gtgcagtagt cggaggcaca gccgcagcaa tcgccgcgtt
    14341 attgttagca ccaaaatctg gtaaagaact tcgggatgaa ttagctgccc aagcggatga
    14401 tttgatggat atggcttcag attacacaga tatggcgaaa gaaaaatcga ctgagttaac
    14461 aggtattgca aaagaaaaag cgcaagatct ttctcaacaa gcaggtgatc tagcaggtag
    14521 tgtcaaacaa aaagtaagtg aatcagcaga tgaagcagga agtgaatctg acgatatctt
    14581 gttaaatctt aaagaacaat cagctgactt atctaatcgt ttcaaaaaaa cggcagaaga
    14641 tatgaaaaaa cctgcagatg aattaggaag tattgctaaa gatacttcag aagatatcat
    14701 gattgatgta aaagacgctg ctggtaaagc aggagatact gtcaatgaag gaatgtcaga
    14761 agcaaaacca gttgcagatg actcaaaaga aatgatgaaa atggcaaaag aagatgcgaa
    14821 agaagctaaa aaagaaataa aagatacaat gacagattca tctacaaatc actaaaatca
    14881 caaatttata gaataaaaag gtgggagcgg actagctctc acctttttta ctcttatttt
    14941 tctaaaacaa gtaattcttt tggtgtagtc gtgaatggca cacagccatc gcttgtcaca
    15001 tatacacagt cttcgatacg tacccctact ttttcaggaa tatagattcc tggttcaaga
    15061 gagaagcaca tcccttcttc gattactaag tcatttcctt ctacaaggga agggaattcg
    15121 tggacggttg ttccgatccc gtgacctaaa cggtgattga aatattcgcc gtagccatag
    15181 ctgttgatca cgtttcgtgc gatttgatca agttcacctg ctgtcacacc tggacggacg
    15241 gcttcttgtg cggcaagctg tgcttcaaga gtgatgttgt agattttttc ttggaagtcg
    15301 gttggttttt gataagcaat tgtacgagta gcatcactgc aatagccatt ccaaacaacg
    15361 ccaagatcga ataacacaag ttcattcggt gaaatcttcg tatttcctgg tgtaccatga
    15421 gggcttgcag catttgctcc tgcaaggact aatgtgtcga aagacatttg tgaaacacct
    15481 tgttttttca attggtattc aatttctgca atgatcgctt gttcagtcac gccttctttc
    15541 actgctttaa agccaatctc aaatgcaaca tcggcccatt taccagcttc gatcagtttg
    15601 tctttttcct ctagagtttt gatcagttgt agacgttgga tgatggaagt tagatcagtg
    15661 taagaatccg ttcccgtcaa gatatcagag atagcatcga agcgctcgac agtcaaggcg
    15721 ttcttttcaa tccctacatt gccaggctga ccatagcgag cacgaagttc ttttccgatg
    15781 atctcccaag ggttttcgct gtctaaatag ccacggacat catacgccca actactgttt
    15841 tgagcatctt caacttccaa agcaggagtg aaaaggaaag gatcttgttc aagagggata
    15901 aaaagagcta gtacacgctc atgaggatca ctttcgtatc cggaaaagta agcgatgtgg
    15961 ccagggtcgc tgatataagc caattcgact tggtgttgtg ccatccattg acgtagttca
    16021 ttgattttta cgttattcat ttggcaacct actttctaaa ttaattttgt attcaataat
    16081 tgtaccactg atttattaaa agaccaacgt tctaaaaact gaaaaaagta aaaattttca
    16141 tgaaaacaga gataaaacgg ttgcaattct tgagattatt tgatattcta ttttagaaat
    16201 gtaagacgta ttttcagaaa acatataaaa tatgaatagg agaacacaaa tggaaaagca
    16261 aacaatcact atctatgatg tagcgagaga ggcaaatgtt tcgatggcta ctgtttcacg
    16321 tgtcgtaaat ggcaatccaa acgttaaacc agcaacaaga aaaaaagtct tggaagttat
    16381 cgatcgcttg gattatcgtc caaacgcagt tgcgcgtggc ttggcaagta agaaaacaac
    16441 aacagttggt gtaatcatcc ctgatgtcag caatatgttc tttgcttctt tagcacgtgg
    16501 tatcgatgat gtggcaacaa tgtacaaata caatattatc ttagcaaatt cagatggcaa
    16561 cgaccaaaaa gaagtcaatg tattgaataa cttactggct aagcaagtgg acggcgtgat
    16621 cttcatgggt caccatatca cagacgaaat ccgtggagaa ttttcacgtt caaaaactcc
    16681 tgttgtatta gctggctcta ttgatccaga tgaacaagta ggtagcgtaa acattgatta
    16741 tgtatccgca acaaaagatg cagtaaacaa attggcaaaa agcggcaata aaaaaatcgc
    16801 ttttgtcagt ggtgcgttga tcgatcccat caatggacaa aatcgtctga aaggctataa
    16861 agaagcatta agcgaaaata acctaccata tagcgaagga ttgatctttg aagcacaata
    16921 caacttcaaa gatggtctat ctctagtcga tcggatccat aatagcggtg caacagctgt
    16981 ctatgtttca gatgatgaat tagccatcgg tattttggat ggtttattag accgtggcgt
    17041 gaaagtaccg gaagaattcg aaatcatcac aagtaacaat tcactactga cagaagtagc
    17101 tcgtcctcgt ttaacaagta tcacacaacc attatacgat ttaggggcgg tatctatgcg
    17161 cctattgacg aaaatgatga ataaagaaga agtcgaagaa aaaacgatta tcttgcctta
    17221 tggaatcgaa gaaaaaggat ctacaaaata ataaaaggac aaccattcag aggttgtgtg
    17281 cctgccgttt aactctgcgt tacaaggagc aatttcaaaa aacagcatct catgtttgat
    17341 gaatattgtg actcgtgatt cggagttggc aggagaacac cgtttatttg ggaaaagggg
    17401 ttatgacaaa attttgttat aatccttttt ttgtgtctta aaaaagagag aaagtggttg
    17461 aaattacatt tatcgtatat aaaacaatcc gtgtatagat gctgcatttt gttcaccacc
    17521 atcaaaaaaa taagggactg tgacaatact attgtcacaa tcccttatta ctgtgtattc
    17581 tattaaatca atcatctttc tcatctgatt ctttagttgc ttgattcgct cttgcataag
    17641 ctgaagcggt gttccagtaa tctgtatagt tttcatcggt accgccaacg ccaaatttaa
    17701 aggcgctttt cttcggtcca tttttcgccc atagcgattc aactgttttt cctggtgttt
    17761 ggatggattt tttattgaca gtaacttttc caggtgtttg accagtaaat ccagatactt
    17821 ttttcttcat cacatcagat gataattcga atttctcatc agtgccaaaa atgtcaggtt
    17881 ttgcttggta agcttgattg atcagattgg caagataatt agccgaacgt atccttgctt
    17941 tgctgtccat tccagtattg tcatcgtgcc ctgcccagct actgatggta atagacggag
    18001 tagagacaat tagccatgaa tctcggtatt cattggtaga accagttttt ccgacccaat
    18061 ctgcttttcc tagatttccg tttaagcttg agatggcatc tttgaatggt gtagtgattt
    18121 ttgcattgat cacactacgc atcatgtcat tcatgatcga tgccgttgct tccgaataga
    18181 tccggacaga gttgctttcg tgtttataaa ttacatttcc tgcgttatct gtgatggaat
    18241 cgatgatata gccttcttca tagacgccgc cgtttgctaa agcctgaaat ccgttggtct
    18301 gttgaagcgt cgtgacatct actgtaccta aaggagcaga ttcaacgccc caattgtcat
    18361 ttgccgggta gttcatcttt gccaagtagt gctcgtaagc atattgtttc gatccaccgg
    18421 aattcaacac atcttgatat aagtgataag ccgggatatt gttggaccat tctagtgact
    18481 cacggacggt ttggaaggtg ttcgatcctt cgttagtagc attgacgatt ttctcgccag
    18541 cattttctcc ttcttgccaa gttgttgcat aatctgatac acgggattct gatccgataa
    18601 gccccatgtc gattgcaggt ccataaacaa gaactggttt gattgatgag ccagcttgac
    18661 gttctgtatc gaaggcatgg ttattttgat tctgactgta attccgtccg ccaacgaatc
    18721 cgtagatgcg gccagttctg ttatccatca atacatttcc ggtttctact tgactgccag
    18781 caccatcatc taacatgtat ccataatttt caacgccatt ttgcaatgca gcatagacat
    18841 ctttatcgat tgtcgaatgg attgtatatc ctttattttg gatcgtttgc tgtgcctttt
    18901 gataataagc gtcactgacc gatgaatcag acatatcagc gttgtctttt tcagccaatt
    18961 gttgtgcaat gattcttgtt gcttcattca tcacagtata atacaagaat tctctgtcag
    19021 tctgttctgc gatttgttgt ggtagaaagt cttttgtaag gtcataggct tttgcttctt
    19081 cgtattgttc tttcgtaatt tggccttcac gatacatact gaataaaaca aaatcttttc
    19141 tggcaaggcc ggcagaaaga tcttctttta gcgcgcctgt gttcgtgtat ggactatacg
    19201 tgatcgggct ttggggtaat ccagcgatat aagcagcttg cggcagcgtc acatcttttg
    19261 catctacacc aaagattcct tttgcagctt cctggactcc agcaatgttt tgtcccttat
    19321 tattacgacc aaatggtgag acattcaaat acgtagcaat gatctcatct tttgaaaagt
    19381 atttttctac ttgtgcagaa agaatgatct cgtttgcttt acgtttgaat gttgtttcat
    19441 ctgtaaggat ctgctgtttg actaattgct gtgtcaaagt tgatccacca gaagagccaa
    19501 taccagtagc ttcagatact aatgctcgta aaacagcttt aggaacgtaa ccttggtgtt
    19561 tatcgaagta ttcatcctct gtgctgataa ttgcagtttt caataaaggt gaaatcttat
    19621 cagatgaaat agtcgtgcgc atcagatctg tctggatttt tgatatctct gtattatctg
    19681 cataaacaat ctttgacgtt tctgtaatgt ttcctagttc tgtctgcagt gcttttttag
    19741 tcggcaattg tgtgtcttcg acgagatagg caaaatagcc agcgcctatt ccaataccta
    19801 gagctgcgaa catcaaaaga agaacagtaa taaaaacagt caatgattga aaaactctaa
    19861 aaataatctt aggaacaaac catttttctt tttttcttct attctgtttt tttcgagtat
    19921 ttttgcgtgg caaaagggtc acctcaacta atggttttat ttcgctaatt atagcaaata
    19981 atagaacaaa aaaacagaca aggctgtttt tggcaggaat ttctttttta aaagagttaa
    20041 aacgtgagaa aatgatcttt attataaaag agttcttaag cttttcttta gagaaaattt
    20101 cagttactat ggagaacagg gcaaagaaaa atcaatcatt ttctttgtat atcatcttac
    20161 caatatatag gaattgttag taacaattgc ctttcaagga ggaatccatc catgttatta
    20221 ggctcacacg tcagcatgag cggaaagaaa atgctgttag gagcagcagc agaagcagcc
    20281 agttatggat cttcgacttt tatgatctat acaggcgccc ctcaaaacac tagaagaaaa
    20341 gccatcgaag agatgaatat tgatgccgga aaagcgtaca tggcagaaaa cggtctaaca
    20401 aatatcgtcg ttcatgcccc ttatatcatt aatttgggga atacgatcaa accagaaatg
    20461 tttccttttg ccgtccaatt tctgagagaa gagattgttc gtgcggaagc tttaggcgct
    20521 actcaaatta cacttcaccc aggtgctcat gtaggggcag gaacagaagc cggaattgct
    20581 agaattattg aaggcttgaa tgaagttttg acaaaagaac agacggcaca aatcgctttg
    20641 gagacaatgg caggtaaagg tacggaaatc ggtcgaacat ttgaggaatt agcgcaaatc
    20701 atcgatggag taaaattaaa tgaaaaatta tccgttactt ttgacacctg tcatacgaat
    20761 gatgccggtt ataatattaa agaagacttt tcaggtgtaa tggaagagtt tgataagatc
    20821 atcgggttag atcggcttaa agtactccat atcaatgatt ctaaaaatcc gcaaggttcg
    20881 cataaagacc ggcacgccaa tattggtttt ggaacaatcg gattcgacac gctgaataag
    20941 atcgtccatg atccaatgtt tgacaatatt tcgaaaatct tggaaactcc ctatgtcggc
    21001 aaagataaaa agaaagctta cgcaccatat ggaaaagaaa tcgccatgtt caaagcacaa
    21061 atattcgatc cagaagtgtt tcctgagtta ttagcagaag aagtgatact ctaagatttt
    21121 ctatctgaca gcagcaaaaa aggaccaggc acattgcctt gtcctttttt gtttaagaaa
    21181 caaaggagta ctttttgcag tctttttcat catgcatctt ctcccccctc tttttaaatt
    21241 tagacaaaca aggtatactg ttagaaaagt aaaaaaacga aaagtttcac aagcaagtgg
    21301 caaaacgtga ttggaaggac gaaaagatgc tgaacacttt aaaacccttg atcaaccaac
    21361 taaaaaagaa acatacatac tttattcttg gcatagccct attatttaca attattcgtt
    21421 tgtatttata tagtaaagca atctatggaa cagacatgtc ggcaacctat gatgaccagc
    21481 tgctgattcg ctatgctcaa aatattttgg acggtaaatg gctaggggag tatacgacta
    21541 caacactttc caaaggaatc tcctacagct tatttttagt acttgcacat aaactgcatc
    21601 tttcttatgc tgttttgttc ggcatgttca gtatttttgc cagtatattg gttgcactcg
    21661 cgtttaaacc gatcgtgaag aacaagtatc ttcttttaag tatctatttt tattttttgt
    21721 tttctccgat tagttttaca catgaatatt ccactcgaac atatcgtaat gccctcgtga
    21781 ttccggctgt tgagatcatt atcgcttgtt tactagctat ttattaccgc aaatacggtt
    21841 ctagcagaaa attgctgcca tggtttttag ggctttctgt cgcttttcct ttcttttggt
    21901 tcattcgcga agattctatc tggcttcttc cttttactgt agtagctttg ggtatcagtt
    21961 tcgtccagat cttgctaggg caagactttt ctttcaaaga agtaaagaag ttaaaaaaac
    22021 gactctttct tctctcgaaa cgggaatggc tgaaactttc attgttttta cttcctatag
    22081 ttggcttttc catcgtctat cagtggatcg aatataaaaa tgagacaaca tacggtattg
    22141 caaccatcaa tgaccgttcc agtggagaat ttggacgttt gatgaaacaa ctgatccgaa
    22201 tagatgatga aacgaacttg aataaagaaa acagtgagat atgggtctca catgctgcac
    22261 taaataaagc attagccgca tctcctacat tccatactgt ttctacccgt attgacgaat
    22321 tatatacgac tcacgcgtgg actcaaggtg gaaaagtaaa agaattaccc ggagatatca
    22381 tcttttgggc attgcgagat gtggtaagcg aatctggtta ttaccaaaac aatgcaaaag
    22441 aaaccaatga tttttggaaa aaagtcaacc aagaattaga agaagcctat cacaagggaa
    22501 ccatcaaaaa gaaaaaagag ttctatctga tgggatcagg agatggaaag acgatgagtg
    22561 atctgccaat cgtattcgat ttccttcaat caggatacgc ttatgcatgg cgatataaag
    22621 atttccatca aggaggaaat tatagtttcg gaacgcaaga acaagtcaaa caggctcaag
    22681 aattattaaa ccttccgcta atggagaatt ggatcgatag taatgaaccg agaacaccta
    22741 atttccaatt aacaaaatca gcaaaagtcg cgaatgtcat cataaaaatt taccaagttc
    22801 tggctattcc cgtgctgatt gcttcaatag cagggatatt acttatgata ctaggaaccg
    22861 tatttgcaaa agctgatcgt gcctattata gttcctcttt gattgtggtc accggattaa
    22921 tactgacaca attggttttt cttttcggtg tctcgtggtt ttgttcttat gcgccagaaa
    22981 aaaaggatta ttttttaagt gtctacactg gcgcaggcgt gccattaacg caattcatcg
    23041 tagtaacaag catgctagtt ttcagtaaat taaatctgaa aaaggcttta tcttctaaat
    23101 aaaaagaccc ttggtagcta gatttttaac ctaactatca agggtctttg tgtggaaaaa
    23161 tcagtttcta ttcgatttct tctaccgttt cctgcaaacg ctgttcatat tttttgtttc
    23221 ctttatataa atcaccaatc gtccatgcca acaggagaag gacaagaaca atgattacat
    23281 aagcgatgtg atcagccaat gctagttgag cagaagtggc ttgttcacca aagaaacttt
    23341 cgatctgtcc tggtaatcct cgtaagttca agtaagttag actaatcaca gagaaccagc
    23401 caagaagttt tacgatgaat gaatttttaa atcggctacc catctccgct tggctgttgg
    23461 tcatcatcag taaagggatc atcgaaaacg gcaacgcaaa agccaagaac acctgggatt
    23521 cattcatcaa gttattcaaa gcggtatgtt cttcgatcgt tccttttgta cttgtaaaca
    23581 aaacacaaat caatacagga atcacagaaa gcaatcgtgt gaccagtctt cttagccaaa
    23641 tcgggatacg catatggatg aagccttcca tgatgacttg accggtcaac gtaccagtaa
    23701 tagtagagtt ctggccagaa gcaagaagtg ctacagcaaa taaagtggat aagataccag
    23761 attttgctac agcaatcaag ataccattgc tcatagctga tggatcagat aaggcttcat
    23821 acagaccaaa aaaggaagga tcttggacag aaccggtttt gaagacagcg acacccatga
    23881 tcaatagcag tgcattgact agaaaagcca tcgacaattg gatattagag tcccaagtag
    23941 aaaaacgtac agcttgtgct acatcttctt cgtcttggtg atcaatcttc cgagtttgag
    24001 aaactgctga atgaagatat aagttatgcg gcatgactgt tgcaccaata atccctaaag
    24061 ctccagtcag cggagtttcg ccgccgatga ccggtgagtc tgagaatgta tgactgttag
    24121 ggataaagcc agcaaataaa ctgtgccaat ctgggttaga caagccaact tgatagacaa
    24181 aaacgaataa aatgacgaaa attaagcaga caaccagtgc ttcgatctta cgaaacccga
    24241 cttttgtcag tagtaagaga acgaaaacat ctaatacagt aataaagacg gcaatcacca
    24301 atggaatatc aaaaagtaag tagagggcga ttgctgcacc aatgacttct gcgatgtctg
    24361 ttgccataat ggctaattca gtcaagatcc ataaaatgac acccaatgtc ttacttgtgc
    24421 gtgctcggat tgcttgggcc aaatccattt gggtcacgat tcctaatttt gctgccatat
    24481 actgtaaaag catagcaatc agactagaca tcaaaataac tgacatcaat aaatattgga
    24541 aattttgtcc accagtgatc gaagtggacc agttacctgg atccatataa ccaactgcta
    24601 ctaatgcacc tggtccagag taagcaagaa gtgttttaaa aaaacttttc ccctttggta
    24661 catcaactgt tccatttact tcagctaaag ataaaccatt tgtatgttca ataagtcgat
    24721 gcttatgaaa ttgtttgttg gattgattat tcaagaaaaa accacctttc ctatttatct
    24781 acgattacat tctacacctc aaaaagaaaa aattaaagaa aaatattcgg tatgcctaat
    24841 ttattttcgt ggcgcgtctt gttagatgac gaaagaaatt ttgttctcct ctaaaaaaac
    24901 cactattttt tgcatgaaac taaagaatac gcctttcttt tttcaataaa ctttagtaaa
    24961 ataagtcatt gagagcaatt cggaaataga aaggagagat ttatatgtta tcaacaggta
    25021 ttgtcgtact tattgctatt attgctttgc taatcggcgc agtaggtggc tttttcctag
    25081 cgcgtaaata tatgaaagac tatttagaaa aaaatcctcc cgttaatgag gacatgttgc
    25141 gatcaatgat gatgtcaatg ggacaaaaac catctgagaa gaaaattcgc cagatgatgc
    25201 agcaaatgaa gaaccaaggg aaaaaatagg ttctgaccca acgacttttt acgggctgat
    25261 tttttcagtc cgttttttat tttgtaattt gttagactat tcagattatt cagcaaaaag
    25321 acaagcacag acaatcctag gctcttgata aagacaaagg aggagtaaaa tgtcgatttt
    25381 tagaaagtta ggctggtttt ttcgtcagga aaagaaacat tatttaattg gggttttctc
    25441 gctattcgca gtggccttgg tacagttagt tcctccaaaa gtgattggga tcatcattga
    25501 tgagattgcc gataaaaatt tatcagtaaa gatcattttc gggtggatct tcctattgat
    25561 tatcgctgct ttcttacaat atctttttcg ttatatctgg cggacgaata tttggggaag
    25621 tgcagctcgg ttggagaaag aacttcggca gcagttattc aaacatttta caaaaatgga
    25681 caatctcttt taccaaaaat atcggactgg ggatttgatg gctcatgcaa cgaatgattt
    25741 gaatgcgatc cagaatgtag caggagctgg tatcttgacc tttgcagatt cagtgattac
    25801 gggcggaaca acgatcattg tgatgatttt gtttattgat tggcgcttaa cactgattgc
    25861 gctgattcct ctgcctttgc tggccgtcac ttcacgagtg ctggggtcga aactccatga
    25921 tgcttttcgt gattcgcaag aagctttttc tagtatcaat gataagacac aagaaagtat
    25981 cactgggatc aaagtcatta aaacatttgg acaagaaaaa gaagatctag cggattttga
    26041 agaaaagatt gaagatgcaa ttattaaaaa caaacgagtc aattttctag actccttatt
    26101 tgatccattc attaccttga ttatcggtct gtcttatgtt gcaacaatta ttttaggtgg
    26161 aaagtatgtc ttggaaggca cgatctcgat cgggcagatg gtctcattta ttagttatat
    26221 cggtatgctt gtttggccga tgtttgcgat tggtcgattg ttcaacgtat tggaaagagg
    26281 aaatgcttct tatgatcgcg tcaatgaact tttgcatgaa aaaacgcata tcgtggaaca
    26341 actgggtgca gtgcagaccc cggtaaaagg cgagctgaat gtagctattt cctcttttaa
    26401 ttatccgaaa gaagaatcac cagcattaga acaaatcaag tttgcaatcc aagaaggaga
    26461 aacattaggt attgttggga aaactggtgc gggaaaaaca acgatcatca aattactgat
    26521 gcgtgaatac gatgattatc aagggaaaat ccaatttggc ggacgttcga tcaaagaata
    26581 ttcattgaat gctttattag gatcaatcgg ctatgtgcct caggatcact tcttgttttc
    26641 gatgaccatt cgagataata tccgtttttc taatccaaaa ctttcagaag aagaagtaga
    26701 gcatgcggct tctttagcat ttatcaacaa tgaaatcaag gaattaccaa aaggttatga
    26761 taccttagtt ggagaacgtg gagtctcttt atctggcgga cagaaacaaa ggatttcgat
    26821 tgctcgagca ttgatcaaaa atcctgaatt attaatatta gatgatgcac tttctgcagt
    26881 agatgccaaa acagaagaag ctatcttgca taatttaaaa gaaataagaa aagagaaaac
    26941 aactatcatt gcagctcacc gtttaagtag cgtgatgcat gcaaaagaaa tcattgttgt
    27001 cgatcaaggg aaaatcattg aacgaggaac ccatcatgag ctgcttgcac taggcggctg
    27061 gtatgaacaa atgtgggaga aacagcaatt agaagcgaag atagaaggag gcgaggcctg
    27121 atggatgaaa attatcaatc ggaatggaca aaatcgattc cacttaaaga acagaaaaag
    27181 atcatcaaac gactgatgaa gttcgccaaa ccttttagaa caacgttcat cgttgctatc
    27241 ttatttgcgt ttgctctgtc tgtgatcaat gtcctattgc caaggatcat ccaaactttt
    27301 atggatgatc atctggctaa acagtcggct acgacacaag ttattttgtt ttttgctggt
    27361 atgtacttat ttggagtcct agtaaaaagt atcatttggt ttttccaatg gtatttgtat
    27421 tccatggctt cattgaagac ctatcagtat attagggtaa aattatttga aaagctgcat
    27481 acactaggta tgcgttattt tgatcagaca ccggcaggat caaccgtttc tcgagtgacg
    27541 aatgacacag aaacgctatt tgaattttgg tacgtctttt tgatggtatt gactgggatc
    27601 tttgcagtcg tttcttcatt cattgccatg tttcaaatca atggagaaat tgccttgtgg
    27661 actttggtgt tcttgccgat tttaggaatt gtcatctggt attaccagaa atttagttct
    27721 cgtatttacc gaaatatgcg ggaaaaatta agtcagctga atacaaaatt gaatgaatcg
    27781 atttccggca tgcgtattat ccagcagttt cgccaagaag ctcgtttagc taaagaattt
    27841 gaacaagtga acaatgagta tttagacacg cgctatgcga tgatcaaaac gaattcgatt
    27901 ctgttaagtc cgatcatcaa ttttctatat gcattggcga ttgcgttggc gttgacttta
    27961 tttgggatcg atgcgttgca ttctcccgta gaagttggac tgatctatgc atttacgact
    28021 tatgtccagg gattttttaa tccaatgtct caaatgatgg actttttgag catcttcaca
    28081 gatgggatgg ttgctggtag tcgtatcttg aagattatgg atacggaaga atatgcacca
    28141 caacaaaacc ctggagcaga tcaaatgatt acttcaggta agattgagtt tcgcaatgta
    28201 agcttttctt atgatggaga gcacaatgta ttaaacaata tctcttttgt tgctaatccg
    28261 ggagaaaccg ttgcattagt gggacacacc ggaagtggga aaagctcgat catcaatgtg
    28321 ttgatgcggt tttacgaatt ttatgaaggt cagatattga ttgatgatca tgacattcga
    28381 gaatatccaa tggaagagtt acgtaagaaa atggggctgg tcttgcaaga tgctttcttg
    28441 ttttatgggg atatcgcagg aaatattcgg atgatgaatc cagctatcac agacgaacag
    28501 atacgccatg cggctgagtt tgttcaagca aacaaattca tcgatgaatt accaaaagga
    28561 tatcactcaa aagtgatcga acatggtgcc agctattcta gtggacaacg acagctgatc
    28621 acttttgcgc gaacaatcgt gactgatcct aaaattctag tattggatga agcaacagca
    28681 aatatcgata ctgaaacaga aggattgatc caagaaggat tggcgaaaat gcgacaagga
    28741 cggacaacga ttgcgattgc ccatcggctg tcaacgattc gagatgcaga attgatttta
    28801 gttttagaca aaggtcatat agtagaacgc gggaatcatg aaacacttct agcgcaaaat
    28861 ggtttgtatg cagatatgta caaactgcaa agtagtggta tataaaagat gttgtgacag
    28921 tagcattttg acctaagcac tagagtgaaa ataaaacata gcttcccata tttttcatct
    28981 gtgagagtta gtgtcgtagt taccagattt aaaaaagacg acgtcagcta gtcagaagtg
    29041 cctaatagcc cctaagaaag cgttgacaaa atcgtttgct ttcgataata taaagaaaac
    29101 gatacaactt ggatagtgat tgcctgatgc aagctaaacc tgatgagtct cccatggttt
    29161 catggacggc tttttgggtt tttctttttg ttttggatac agccctatat atcatggaaa
    29221 ggagcaaaac atgctgattg agaaagtact gaataacaac gtcgtaataa caagggatga
    29281 aagagatcgg gaaatgattg tgatgggaaa aggattggca ttcaaaaaga aggtgggtga
    29341 cccgattctt gactcatgca tcgacaaaaa atttcagttg acaaatgacg gactttcaca
    29401 acagttccaa gaattggcac aagcgattcc tcttgagtac ttagaaattt cttgtgaaat
    29461 cattgaatat acaaaagagg ctctgcaagt ccatttaaat gattctgtgt atatttcatt
    29521 gacggatcac ttgtatacag ctatcgaacg tcaaaaaatg gggatctttg tgccaaatgt
    29581 attgctgtat gatatcaaac aactgtttcc taaagagtat gccattggga aaaaaacagt
    29641 gaagaaaatc tcggataaat accacatcaa gttatcagaa gacgaagccg gatttatcgc
    29701 attgcattta gtcaatgcac aaggcattca aagccagcaa gacaatacct acctgtttac
    29761 cacaacaata caggatattt taaatatcgt ccgctattac tataagatta catttgatga
    29821 agattcagtc cacttttttc gattcgtcac acacctgcgt ttttttctga cacgtgtcct
    29881 agatggagcg aaatcagaag atggagaagt ttctgaggaa ttgttccaag ttattcaaaa
    29941 aaaatataag gatacgtttg gttgcgttga gaaaatagca gactttttaa ccggggcttt
    30001 cgactaccag atgtccacgg atgaaaaaat gtatttaacg atccatactg cgcgtttagt
    30061 gcatgagaat cgttgtaaat aaataaaaaa ggaggacaaa ttaacaagaa cgtatgaaat
    30121 aagggtagtg acattcagtt ggctaaacct tccactaatt aattggaggg aatagaaatg
    30181 ggaaaatacc aggaattagc agaaaacatc gtaaagaata tcggaggaaa agaaaatatc
    30241 cgtagcttga cacactgtgt gacgcgactt cgtttcaaac taaaggagga aaataaagca
    30301 aaggatgatg ttttgaaaaa gatggaaggg atagtcacca tcatgaaaag cggcgggcaa
    30361 tatcaagttg taatcgggaa tcatgtgcca gatgtctatg cagaagtact gacagtagcc
    30421 gggatttccg atgaagcaga agaagaggtt tcttcaagta gtaatctgtt caatcgatta
    30481 atcgacatat taagtggctg tttccagcct tttttaggtg ctttggctgc agcgggaatg
    30541 gtcaaaggat taaatgcttt gttcttgttt ctcaaatttt atactgaagg atcgggtacg
    30601 tacatcatgc tgaatgggat aggggatgca attttttatt tcatgccagt gatcttaggg
    30661 tacacagcag cgaaaaaatt tcatctgaat ccgatggtgg ggctcgtgat tggtgcagca
    30721 ctttgttatc cgactgtaca aggagcagca ctacaaagtg gtttccctga tggcagtcct
    30781 gcgccttata gcttgctggg tcttccggct tatgaaacat ttttgggtat cccatgggtg
    30841 ggcagcagtt atactagtag cgtcgtacct gttatcttca ttatcgcatt tgccggacaa
    30901 gtccaaaaaa ttgccaagaa atggattcct gcagtcgtcc agaccttttt ggtttctttt
    30961 tctgtgttat taatcgcact tcctgctggc tttttgatta tcggtccgat tgtcagtata
    31021 ctgacagatc tattaagcac gggattccaa gcattgatgg acttttctcc agctgtgtat
    31081 ggtgcagttc tagggtttct ttggcaagta ctggtgattt tcggacttca ttggagtatt
    31141 atccctcttg cgattatgca gctagcgcaa aatgggtgga gtcagatgtt agtaggcgtt
    31201 tatggcgcga gttttgcaca gacagcagtg gtattggcga tgtatttcaa gttcaaggat
    31261 caaaaaatga aggcgctttg catgccagct gtgatttcag gaatcttcgg cgtaacagaa
    31321 ccggctattt atggaatcac cttaccgaag aagaagccat ttatttactc gatgattggt
    31381 ggtgctgttt caggaatcta tttgatggca tctggaacaa tcagctacat aatgggcgga
    31441 ttagggattt ttggggtggt aaactacatc acggaaacag acgccagtgg tatgtttcaa
    31501 gcgtttatcg gcattgctat tgcttcggta atcgggtttt cgctgacttt tttcttttgg
    31561 aaggatgaag caggtgaaac agatgctata cagaaaaaat caggtaaaga aattgtaaaa
    31621 acaccgatac aaggacaaat cgctccacta aatgcagcga aggatgcagc ttttgcacaa
    31681 ggaacactag gtcgaggaat cttaatctac cctgaaaaag gagaagtacg tgcccctttt
    31741 gacggaacag ttatgaccct gttcccgacg aaacatgcaa tcggaatggt ttctgaaaca
    31801 gggctggaat tgctgattca tgtcggattg gatactgtgc agttggaagg gaaatatttt
    31861 gaaagcttag tgcagcaagg agaaagagtg agacaaggcg atctgttgct acgcttcgac
    31921 aaaaagaaaa tcgaaaagga aggatacagt ttagaaacac ctgtgatcgt caccaattac
    31981 acggattatt tggatatttt ggaaaaccaa ggggagacgg tcgggccaga cgatgcactt
    32041 atcaccgcac tgacctgaac ggttaggtga agaatggact aggcttaact attcttttta
    32101 ttccacttct ttattttcaa aattgaaagg ctctttcatg atggctgagc tgatagggcg
    32161 tttatcgaat ttttgaccga cagaaattag atggactaaa ttctgttgat aattttctgc
    32221 aaaaacacaa ctatataaca cgtctagaag ataacaaatt gatgtattga tcgtaaaatt
    32281 accaattttt gaatacagtc gctcgcgtgt cgtaagtcgc aaagcggctg tactcattgc
    32341 agatagcgtt gaatcaccga tactggtcaa agctaaaatt ggaacatcat tttgtttcaa
    32401 tatttctgcg gtacgaagga tcacattgtt ttcacccgta taagaaatca gaatcccgca
    32461 catatcgctc gtacaattgt acgcttcata gaatgattca ccaaaagtgg tgacaattgt
    32521 gacatccatt ttgatgcgct tcattttcag catgaaatct tgagaaatca aagtattcga
    32581 gttactggcg aagatcacga ttttttttgc ctggagcagt aaatatttag ctttttgcag
    32641 ttggtcatga tgaatgagag aaagtgtatc atgaatcgtc atctgttcta aagaagccat
    32701 tttatttgcg attgtcataa taccgtctgt ggtggaaaaa ggaagattcg catctacgtc
    32761 ttgaaaattc ccttcaagat aggctgtttc agctaaaaaa gcattacgaa aactgaccca
    32821 gccatcaaaa cccatttttt ttgctacacg gatgagtgta gacgggtgga cataacatcg
    32881 ctcagctatc gcttggatgg tcatttcgcc aagtgctgga gaatcgctta agagaaactg
    32941 gatcacttca ttttctgaag gagaaaagga gatttctttg attttttcac gtaataacat
    33001 gtagatacct tcttcttatt tttttacgta aattataact tttttgaaaa tgttttgtaa
    33061 acggatttat actatataac ttaaaatagg ctttttttga caagatattt acaaaaatcc
    33121 actagaaaac agtaactttt gtaaatgtta ctggaaagaa acggatttct attgtttgtt
    33181 ttgtttacaa aaaagataat gaaagtgtaa aaaagtaaag gagtgagaac atggcaaaag
    33241 gaatcaaaat cgctacgatt ggtggaggca gcagttacac ccctgagttg atggaaggat
    33301 ttatcaaaag atatgaggaa ttaccgatac gagaaatctg gctcgtagat gtagaagaag
    33361 gaaaagaaaa gcttgagatc gttagtcaga tgtctcagcg tatgtgggat gcttctccat
    33421 atgaagtcaa gatctatgca actctggatc gggaagaagc attgaaagat gcggattttg
    33481 ttacgactca gtttcgtgtc ggtcaattag atgcacgaat caaagacgag cggatcccta
    33541 gttattatgg gatgattggg caagaaacaa atggtgctgg cgggatattc aaagcttttc
    33601 gtaccgttcc agtcgttttg gcaatcgttg aggacatgaa gcgtttatgt ccaaatgctt
    33661 ggctgatcaa tttcgcgaac ccaagtggga tggtgacaga agcagttatc agatatggca
    33721 aatgggataa agtgattgga ttatgcaatg tgccagtgat ggcgatgatg acagaaccag
    33781 caatgcttgg caagaaaaaa gaagagctga tctacaagtt tgccggtcta aatcatttcc
    33841 attggcacaa agtagcagat aataaaggaa atgaccgcac ggcagaattg attgatcgtt
    33901 tatacgatga agataatgga ttaccgaaaa atatctttga tatcccgttt ttcaaagaac
    33961 aattaaaaca aatgaacatg atcccttgtg gctaccaccg ctattactat cgtgaagaag
    34021 aaatgctgga acatgcttta gaagaatatc aaacgattgg tacaagagcg cagcaagtga
    34081 aagaaacaga agcagagcta tttgaattat acaaagatcc agaattagac cataaacctg
    34141 aacaattgca aaaacgaggt ggcgcttatt attcagacgc tgcctgtgaa acgattgctt
    34201 caatctatgg aaataaagaa acacagatcg ttgtatctac aaagaatgat ggagctgttc
    34261 ctgacctgtc ggcagattgt gttgtagaag tcactgccta tgttggcgca caaggagcga
    34321 gaaatgtagc gtttggttct cttcctgctg ctgaaagagg ctggttgcaa gtaatgaaga
    34381 atatggaatt gctgacgatc gaagcagctg tgactggtga ttatgggaca gctttacagg
    34441 cgtttacgat caatccactt atacgatcag gaaagacagc aaaacggatc atggatgagt
    34501 tgtttattgc acaccagcag tatttaccgc aattcaagga aacaatcagc cgattaaaag
    34561 aagaaggaat cgaagttcaa gatgaactgg caagagaatt atcaggtaat acacatataa
    34621 tggaggaagt ataatggggt tcagaaaaga ttttttatgg ggcggtgcta cagcagccaa
    34681 tcaatgtgag ggcgcttatg atgaaggtgg acgcggacta gcaaacgtag atgttgtgcc
    34741 aatcggaaaa gaccgttttc ctgttatcac cggaaagaaa aaaatgtttg aatttgatga
    34801 ggatcatttt tatccagcaa aaattgcaat cgatatgtat catcgttata aagaagatat
    34861 tgctttgttt gcagaaatgg ggttcaagac ttatcgaatg tctatcgcct ggagtcgtat
    34921 ttttccaatg ggggatgaga aagagccgaa tgaagctggc ttgaaatttt atgaggatgt
    34981 ctttaaagaa tgccaaaagc acggaattga gccacttgtg acgattacgc attttgattg
    35041 tccaatgcat ttgatcaaag aatatggtgc gtggcgttcc agagaactcg ttggttttta
    35101 tgaaaatctg tgtcgagtca tcttcaatcg ctataaagga cttgtcaaat attggctgac
    35161 attcaatgag atcaatatga tccttcatgc accatttatg ggcgcagggc tgtattttga
    35221 agaaggagaa aatgaagaac aaatcaaata tcaagcggca catcatgagt tggttgcaag
    35281 tgccatcgca acgaagattg ctcatgaagt agatccggag aacaaagtag gctgtatgct
    35341 ggcagcagga gcatattatc catacacttg caatcctgaa gatgtttggg cttctagaaa
    35401 atcagatcga gaaaattatt tcttcattga tgtccagtca cgaggagaat atcccgctta
    35461 tgcattgaaa gaaatggagc gaaatggcat tcagatcgaa atggaaccag gggatgccgc
    35521 attgctgaaa gaacatactg ttgattttat ctctttttct tattattctt cacgagtttc
    35581 gactaccgac aacgaactct tggaacagac tgcaggaaat atctttgctt ctgtcaaaaa
    35641 tccatatctg caagcaagtg aatgggggtg gcaaattgat ccattaggct tgagaataac
    35701 gatgaatgat atttatgatc gttatcaaaa accattattt attgtagaaa acggcttagg
    35761 tgcagtcgat acgccagatg aaaatggtta tgtggaagat gattaccgaa tcgattacct
    35821 ggcagcgcat atccaggcga tgaaggatgc agtagaacta gacggagtcg atctattagg
    35881 ctatacgact tggggatgta tcgatttagt atcagctggt accggtgaga tgaagaaaag
    35941 gtacggattc atttatgtcg atcgtgataa ccaaggaaat ggcacattag cccgttcaaa
    36001 gaaaaaatca ttcaactggt ataaaaaagt aattgattca aatggagaaa atctttcgaa
    36061 caattgatca atgatttata aaagtctatt gtttttcagc ggatttttcg agtataataa
    36121 agctattcga gaaacgcaag cagaacaata gacttttttt cgcattggct gtaaagtatg
    36181 cttacttgat taagtaaagg agaatctaca tatgtatgat gtaatcgtta ttggcgcagg
    36241 tcctgctggg atgactgctg ctttgtatgc ttcacgttca aacttatccg tattgatgat
    36301 cgaacaaggt gcccctggcg gccaaatgaa caatacggca gaaatcgaaa attatcctgg
    36361 attcgattcg atcatgggcc ccgatctagc ttataagatg tatgaaagtg tctcaagatt
    36421 tgggacagaa aatgcttatg gaatcgttca agatatcaag gatcacggca gccataaaga
    36481 agtcatctgt gaagataaaa gttacgaagg aaaaacagtc attatcgcaa caggctgtat
    36541 ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct ggacgaggcg tttcttactg
    36601 tgcagtctgt gatggtgcgt ttttccgtaa caaacgtcta gtagtcatcg ggggtgggga
    36661 ctcagcagta gaagaagcca tctatttgac acaatttgct tctgaagtag tgatcgtcca
    36721 ccgtagagat gaattacgtg cacaaaagat catccaagac cgtgcatttg caaatgaaaa
    36781 aatttccttt atgtgggata cagtcgttga cgaaatcgtt ggaaacgaaa tggtagtgac
    36841 aggtgtgaaa gcacgtaatg tcaaaaccgg caatgaattt gatttagcag cagacggagt
    36901 attcatctat gttggactgg atccattaac tgcacctttt agagaagcag gtttgacgaa
    36961 cgaagctggc tggattccaa cagacaacga aatgaaaacg aatatgccag gcgtttttgc
    37021 catcggagat gttcgtgaaa aagatttgcg acaaatcact acagctgttg gtgacggcgg
    37081 tattgctgga caacaagtgt ataaatactt ggagtcttta gaagaaacaa cagcataagt
    37141 ttaaaaagag tgaaagaacg gtttcaattg ttcttttact cttttttttc gcgtgaaatc
    37201 cactgaatcc gatttatgtg aatacttgca atcatgcttg ataaaagaat cctttcttga
    37261 tttaagcgtt ttttggtcct catactttca tatctaataa gtgaatgcta tacttattaa
    37321 gaaccatatt tcagaaagag gtgttatttt catgtcttgg gaacaagtgt atgaacaatg
    37381 ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc tgggtgaaga
    37441 tcctgaaaaa cttgaagatg ctttttatgc accgttggaa ttcggtactg caggcatgcg
    37501 gggaatctta ggacctggga tcaatcgaat gaacatctat acggtaagac aagcaacaga
    37561 aggactagca cgtttcatgg atacacaagg tgaagaaaca aaacgccgag gtgtcgctat
    37621 cgcttatgac tcacgccatc agtcaccaga atttgcaatg gaagcagcca aaacactggc
    37681 acatcacaat atcccgtctt atgtattcga aagtctgcgt ccaacaccag agctttcttt
    37741 tgcagttcgt catttgaaga cgtttgcggg aatcatggta accgcatctc acaacccagc
    37801 ggcttacaat ggatataaag tctacggaga agatggcgga caaatgcctc ctgcagacgc
    37861 agatgcattg acgaaatatg tccgtgaagt cagcaatccg ctaaaagtcg aagtattatc
    37921 tgacgaagaa gctaaacata gcgggttgat cacgatcatc ggagaagaag tggacgctgc
    37981 ttatctagaa gaaatcaaag cagtgacgat tgatcgtgaa ttggttgaaa caatggggaa
    38041 agatttgaaa cttgtgtaca cgccacttca tggaacaggg aaaatgttgg gcgaacgtgc
    38101 gttgaaacaa gctggattcg aacaattcgt actagttccc gaacaagctg tggcagatcc
    38161 agatttcact acggtcaaat cgccaaatcc agaagaacat tcagcttttg aatatgctat
    38221 acgacttgga gaaaaagagg gagctgactt gctgattgca acagatcctg atgcagaccg
    38281 tttaggcgca gcggttcgtt tgccagatgg ttcttatgaa gtattgactg ggaaccaaat
    38341 cggagcattg tttgctcaat atattttgga agcacatcaa tcaagaggca ctttaccaga
    38401 aaacgcagcg gtattgaaat caatcgtttc cagcgaattg cctacagcaa ttgcggaaca
    38461 ctacggtgct actatgttca atgtgttgac agggtttaaa ttcattgctg aaaaaatcca
    38521 acaatttgaa gaagaccatt cacatacttt tatgtttgga ttcgaagaaa gttatggtta
    38581 tttagttaaa ccttttgttc gcgataaaga cgcgatccaa gcattgcttt tattagcaga
    38641 agtagcagct tactataaaa aacaaggcaa aacgttgtat gacggcttgc aaacgatttt
    38701 tgaaacctat ggctatttcg cagaaaaaac gatttctgtg acaatgagcg gacaagaagg
    38761 ttctgcgaaa atctctgctt tgatggaaaa attccgtaaa gaagcaccga cagaatttgc
    38821 cggagcaaaa gttattcaaa cagaagactt taaagaattg acgagaacag accgtgaagg
    38881 aaacgtagaa aaactaacaa caccgccatc cgacgtattg aagtatacat tggaagaaaa
    38941 tggctggatc gctatccgcc cttcaggaac tgagccgaaa atcaagtttt atattggtgt
    39001 aaaaggaagc tctacagaag acgcagccga aaaagtagcc tcttatgaag cgattatcaa
    39061 aaagatgaca gaagaataaa aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt
    39121 cagtcttttt tcaagttgga ggtgaatgga ttgccagaca cgcaaaaatc aaaagtagag
    39181 aaagtcagtc aattattcaa aatgttgagt gaccccactc gtcttaaaat tctgctatat
    39241 ctgaaagacg gggaacaaaa tgtgacagca atcacccaag cagtggaaat ggagcagtct
    39301 gctgtatcgc atcaactacg tttattaaga gaaaatcatg tcgtcaagtc tcatcgcgag
    39361 ggaaaagcga ttttgtatag tcttgatgat tctcatgtac tggatatttt gaaccagaca
    39421 ctcaaacatg tggaacatca ataacgatag gtgca
//
LOCUS       DASJSZ010000017        38411 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.017, whole genome shotgun sequence.
ACCESSION   DASJSZ010000017 DASJSZ010000000
VERSION     DASJSZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.017"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            508..1431
                     /locus_tag="U4B03_000835"
     CDS             508..1431
                     /locus_tag="U4B03_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2067707.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            1640..2218
                     /gene="pgsA"
                     /locus_tag="U4B03_000836"
     CDS             1640..2218
                     /gene="pgsA"
                     /locus_tag="U4B03_000836"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEN2067708.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            2458..3699
                     /locus_tag="U4B03_000837"
     CDS             2458..3699
                     /locus_tag="U4B03_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEN2067709.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            3808..4857
                     /gene="recA"
                     /locus_tag="U4B03_000838"
     CDS             3808..4857
                     /gene="recA"
                     /locus_tag="U4B03_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEN2067710.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            5268..6824
                     /gene="rny"
                     /locus_tag="U4B03_000839"
     CDS             5268..6824
                     /gene="rny"
                     /locus_tag="U4B03_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEN2067711.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6903..7709)
                     /gene="proC"
                     /locus_tag="U4B03_000840"
     CDS             complement(6903..7709)
                     /gene="proC"
                     /locus_tag="U4B03_000840"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEN2067712.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            7850..10423
                     /gene="mprF"
                     /locus_tag="U4B03_000841"
     CDS             7850..10423
                     /gene="mprF"
                     /locus_tag="U4B03_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN2067713.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            10565..11428
                     /locus_tag="U4B03_000842"
     CDS             10565..11428
                     /locus_tag="U4B03_000842"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEN2067714.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            11673..12611
                     /locus_tag="U4B03_000843"
     CDS             11673..12611
                     /locus_tag="U4B03_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN2067715.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            12634..13314
                     /locus_tag="U4B03_000844"
     CDS             12634..13314
                     /locus_tag="U4B03_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2067716.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            13287..14111
                     /locus_tag="U4B03_000845"
     CDS             13287..14111
                     /locus_tag="U4B03_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2067717.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            14278..15096
                     /gene="purR"
                     /locus_tag="U4B03_000846"
     CDS             14278..15096
                     /gene="purR"
                     /locus_tag="U4B03_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEN2067718.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            15599..16972
                     /gene="glmU"
                     /locus_tag="U4B03_000847"
     CDS             15599..16972
                     /gene="glmU"
                     /locus_tag="U4B03_000847"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEN2067719.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            17066..18037
                     /locus_tag="U4B03_000848"
     CDS             17066..18037
                     /locus_tag="U4B03_000848"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN2067720.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      18043..18145
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            18211..18780
                     /gene="thiT"
                     /locus_tag="U4B03_000849"
     CDS             18211..18780
                     /gene="thiT"
                     /locus_tag="U4B03_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEN2067721.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(18861..19634)
                     /locus_tag="U4B03_000850"
     CDS             complement(18861..19634)
                     /locus_tag="U4B03_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067722.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            19901..21601
                     /locus_tag="U4B03_000851"
     CDS             19901..21601
                     /locus_tag="U4B03_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN2067723.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            21571..21789
                     /locus_tag="U4B03_000852"
     CDS             21571..21789
                     /locus_tag="U4B03_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEN2067724.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            21809..22699
                     /locus_tag="U4B03_000853"
     CDS             21809..22699
                     /locus_tag="U4B03_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEN2067725.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            22717..23001
                     /locus_tag="U4B03_000854"
     CDS             22717..23001
                     /locus_tag="U4B03_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067726.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            23488..24102
                     /gene="gmk"
                     /locus_tag="U4B03_000855"
     CDS             23488..24102
                     /gene="gmk"
                     /locus_tag="U4B03_000855"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEN2067727.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            24107..24412
                     /locus_tag="U4B03_000856"
     CDS             24107..24412
                     /locus_tag="U4B03_000856"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN2067728.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            24665..27073
                     /gene="priA"
                     /locus_tag="U4B03_000857"
     CDS             24665..27073
                     /gene="priA"
                     /locus_tag="U4B03_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEN2067729.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            27088..27579
                     /gene="def"
                     /locus_tag="U4B03_000858"
     CDS             27088..27579
                     /gene="def"
                     /locus_tag="U4B03_000858"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN2067730.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            27572..28510
                     /locus_tag="U4B03_000859"
     CDS             27572..28510
                     /locus_tag="U4B03_000859"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEN2067731.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            28510..29868
                     /gene="rsmB"
                     /locus_tag="U4B03_000860"
     CDS             28510..29868
                     /gene="rsmB"
                     /locus_tag="U4B03_000860"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEN2067732.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            29881..30621
                     /locus_tag="U4B03_000861"
     CDS             29881..30621
                     /locus_tag="U4B03_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEN2067733.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            30618..32687
                     /gene="pknB"
                     /locus_tag="U4B03_000862"
     CDS             30618..32687
                     /gene="pknB"
                     /locus_tag="U4B03_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEN2067734.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            32852..33751
                     /gene="rsgA"
                     /locus_tag="U4B03_000863"
     CDS             32852..33751
                     /gene="rsgA"
                     /locus_tag="U4B03_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEN2067735.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            33764..34414
                     /locus_tag="U4B03_000864"
     CDS             33764..34414
                     /locus_tag="U4B03_000864"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN2067736.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            34415..35053
                     /locus_tag="U4B03_000865"
     CDS             34415..35053
                     /locus_tag="U4B03_000865"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEN2067737.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            35208..36062
                     /gene="mreC"
                     /locus_tag="U4B03_000866"
     CDS             35208..36062
                     /gene="mreC"
                     /locus_tag="U4B03_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEN2067738.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            36066..36575
                     /gene="mreD"
                     /locus_tag="U4B03_000867"
     CDS             36066..36575
                     /gene="mreD"
                     /locus_tag="U4B03_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEN2067739.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            36830..38404
                     /locus_tag="U4B03_000868"
     CDS             36830..38404
                     /locus_tag="U4B03_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN2067740.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT        12631 a         6873 c         8356 g        10551 t
ORIGIN      
        1 ttgcttctca aatttttgtg atatttcggc ttattgccga agaagctact tctggaacac
       61 cgtgtaccaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa
      121 aaacagcttc ttaatgtttt taacttggtt ggacttatta ccgcagatgc aagcttttga
      181 acaccgttta aggaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacata
      241 cggaaaaata tctccttata ttttgccggt ttttgagcta ataccgagga acagctgatt
      301 gaacaccgtt taagaaagag gtcgtgagcc tgccgtttaa ctccgagtca caaggaacaa
      361 gttcaaaaaa cagcttctta tatttattgg atattgtgac ttgtgaccga ggagttggca
      421 ggagaacaat gtttattcgg aaaaaaggag ctgtgacatg acttttgtca cagcacccaa
      481 acataacgtc tttcgttgca acagcagatg cttcggagaa tcaaacagac tggagaagat
      541 gaacgagtgg cttttgaaac catcggagaa aaattacggc aagcacgcct gaataaaaaa
      601 atatctttag atgaattaca gcagattaca aaaattcaaa aacgttattt ggaagcaatc
      661 gacaatgatg attttgacca attgcctggt aaattttacg tgcgggcatt tattcggcaa
      721 tacgctgaag ctgtaggaga agatggagac catttagtcg atgtttttga tgggaaaagc
      781 aaattgacag gatcggtccc tgaacgtccg cagcctgaaa cagtaagtac ttctagaaaa
      841 gcaatgcatc aggaagaatc gcatccaagt cgcttttgga caagtcttcc tgtcatttta
      901 ttaggattgg ttgctttggc gattgtcgtt gtcgtgggct atatgacttg gcaagatcgt
      961 caagctgatc caataatcgg agacacagct tcttcgctta aagtagaagg atcagtcgag
     1021 gagtcctctt cctcagagca aagcacgcag accaaagagt cttctacagt agaatcaaca
     1081 acaaaatcaa gtgaagaaaa gaaaatggca atagctacag gagaagattc tggcagtgcc
     1141 atcgcagtaa atatcacaga cgctaccaag cctgtaaccc ttgaatttac tgctgtcaat
     1201 cgtgtatggt taggtgtgct agtagacaat gcctatgttt atcaagggac actggcagca
     1261 aatgaaacac agagtaccgt gttaccggaa acagcaacaa atgcgaccat cacgattggt
     1321 gcagcaagca atgcaacgat caaagcgaat ggagaatccg taccagttaa tccaggggaa
     1381 aacaatcaga gtccaaaaaa tgtgaacctt actcttcaat acgcagaatg atcgtataaa
     1441 ttagtttacg catctgttta cttgtaggta taaaaaagaa ggatcgtgac aaatctgtgt
     1501 cgcggtcctt cttcgttaat tcaaaaagat caatttcaat aaaaatactc ctttgaataa
     1561 attcatttgg aaaagtaaat aatatcggtg gaaacttcgc tgatagtcac gtagaatagg
     1621 gaagaaagga gtcgatagtg tgaatttacc aaataaatta actgtactta gaatttgtat
     1681 gatcccgatt ttcattttgg ttgtaggttt acctttagat tggggacaat ttaacgtagg
     1741 agacaccaca ctggctgtca cacagctagt aggtgcagtt atttttgctt tagctagtat
     1801 cacagactgg ttagatggaa aaattgcccg tgcgcgaggc cttgtcacta attttggcaa
     1861 gtttgcggac ccgttggcag ataaaatgct agttatgact gcttttatca tgttggttga
     1921 ccaaggaaag gcccctggtt gggtagtagc gatcattgtc tgtcgtgaat tagccgtcac
     1981 aggcttgcgt ttactacttg tcgaacatgg agaagtcatg gctgcagctt ggccgggaaa
     2041 agtcaagacg gctacacaaa tggttgctat tatcttgctg tttatcaata atatcccatt
     2101 cagcctgatc ggtattccag tagatcaaat catgctttat gcttgtctga tctttactat
     2161 ctattcaggt gtggattact tcatcaaaaa cagcggtgtg ttcaaaggat caatgtaagt
     2221 atcgtttagt ggagaggttg tgagcctgcc gtctaactct gagtcacaag ggataatttc
     2281 aaaaaacagc ttttcatgtt ttatgtatat tgtgacttat gaccgaggtt ttggcaggag
     2341 aacactgttt atttggaaca aggagctgtg acaagatttt tgtcacagct ccttgccctc
     2401 tttttaaata aaaaaatata aaaatacagc gtatactata taagggagcg ggaagtaatg
     2461 aaagcagaaa taatagctgt cggaacagaa cttttactag gacaagtcgt gaatacgaat
     2521 gccactttct tgtcagaaca gctagctgat ctaggtatcg aagtttatta tcaaacagtt
     2581 gtaggagata atcagcagcg tttagaagag ttgattgctc tagctgaaac aagaagtgaa
     2641 ctcatactct tatgtggcgg attaggaccg acagaagatg acctgacaaa agaagcaact
     2701 gcggctcacc tcggtaagtc tctcatacaa aatacggaag gatacaaaaa attattggca
     2761 tattttgaaa caacccatcg aaaaatgacc aaaaacaatc tgcaacagtc ccagattatc
     2821 gaaggcgggg ttcctctacc aaatcgtaca gggcttgctt tagggacttt ttatcaaaca
     2881 gatacacatg cgtatatcct tcttcccggt ccgcctaatg agttgaaacc aatgtttgtg
     2941 gaacaagtac gtcctttact agaggaacgt tttccttctg aagaaaaact aatttctaaa
     3001 gttttgcgct tttatgggat tggtgaatcg cgactggtca ctgagctaaa agatctgatt
     3061 gagacacaga tcaatccaac gatcgcacca tatgccaaac caaatgaagt gacattacga
     3121 ctgacagtga aaacaaatga tgttcaggca gggaatcaag ctcttttagc attagaagaa
     3181 aaaatccaag aacgagtagg ggaatatttt tatggatacg gcgatgataa ttcgctagca
     3241 aaagtcgtag tggaactact gaaagaaaac aagcagactg taacagcagc ggaaagtttg
     3301 acagccggcg ccttccaagc cgcattaggc gacattgctg gtgtatctga agtatttcca
     3361 ggcggattcg ttacctactc gctccaaaca aaagctggtt ttctggagat tgatccggag
     3421 ctattagcag aatatggaac agtcagcaaa gaatgtgtgg agcagatggc cattcaagca
     3481 cgtgagatag caagaacaga ttacagtgta gctttcaccg gagtggctgg accggacacg
     3541 ttagaaggtc aatctgcagg aacagtatgg atcgctattt catctaaaga cggcgtggtt
     3601 tcggaaaaat atcactttgc cagagatcgt gctgctatca gatataatgc ggttatgcaa
     3661 ggatttgatc ttttgagaaa acagctttta agaaaatgat aaatgcgaat atttgttcgg
     3721 ttttttcttg ctttttttca ctaaaaaaga tatgataagg ataatgaaat gttgctctga
     3781 tttgtaaata taaggaggat tatcgtattg gcagatgatc gtaaagcagc cttagatgct
     3841 gcactgaaaa aaattgaaaa gagctatggt aaaggctcta ttatgaaatt gggcgaaaaa
     3901 atcgaccaac aaatctctac aattccaagt ggctctcttg cgttagatgt cgctttaggt
     3961 gtcggcggtt acccccgtgg acgtatcatc gaagtatatg gacctgaaag ttcaggtaaa
     4021 acaacagttg cactacacgc tattgcagaa gtacaaaaaa atggcggaac ggccgctttc
     4081 attgatgctg agcatgcgtt agatccgcaa tatgcacaaa aattaggtgt gaatatcgat
     4141 gaactacttc tttcacagcc tgacacagga gaacaaggtc tagagatcgc tgatgcttta
     4201 gtatcaagtg gggctgtaga tatcgtagta gtcgattcag ttgctgcttt agttccacga
     4261 gcagaaatcg acggcgaaat gggtgactca catgtcgggt tacaagcacg tttgatgtct
     4321 caagcattgc gtaaactctc tggttcgatc aacaaaacaa aaacaatcgc tattttcatc
     4381 aaccaaatcc gtgaaaaagt aggcgtgatg tttgggaatc cagaaatcac tcctggagga
     4441 cgcgcattga aattctacgc aacgatccgt ttggaagtac gtcgtgcaga acaattgaaa
     4501 caaggtacag atattgttgg taaccgtaca aagatcaaag tcgtaaaaaa caaagtagca
     4561 cctcctttca aaatcgctga agtagatgtc atgtatggtt tagggatctc acaagaagga
     4621 gaacttctag acatggcagt agaaaaagac attgttgata aaagcggtgc ttggtattct
     4681 tataaagaag atcggattgg tcaaggacgg gaaaacgcta aaatctatat ggctaatcat
     4741 cctgaaatga tggcagaagt ttctgctcta gttagagcag cttatggtat tggtgaagaa
     4801 gtcgctgtcc cagaagatga aaaaggacaa gaagaattgc ctttagtaga agaataaaaa
     4861 ttatgataaa caaaaacggc tggtctgaag agtaggtact ctccttgacc tgccgttttt
     4921 ttattgaata aagaaatcgt gagttattta attatacgag tgggtacata aaataaaaat
     4981 atctgttcat ctacaaagaa taatttaata gaaaagtttc tcaatttaaa ggtttaccta
     5041 tatgaaacta gtgtcattta ttttaaaaac catcgtttaa tgaataagta gaaagccgtt
     5101 tctttgttta tttaaagttg acactacggg gtacaaacat tagaataaag ttgtgcatgt
     5161 tttttgtaca catatatgcg aagatgcata ttgacgagtt tagaataatt tatcatagaa
     5221 gctgtataga tgataaaaat gaaaaagata gtatggaggt ggattttatg gtattcaaca
     5281 ttctcctcgc tatcatcggt ttaattgtcg gtcttggttt agggtttatg gtcgcaaagt
     5341 cacgtcacga gaaagaaata gctggtgcac aatcatcagc tgctggaatc atcgatagtg
     5401 caaaaaaaga agcagaaact ctaaaaaaag aagcattgtt agaagctaag gaagaaaatc
     5461 agaaataccg ttctgaagtt gaaagtgagc tgagagaaag taagttagaa ttaaaatctc
     5521 aagagaatcg tttgattcaa cgcgaacaaa cgctgaatcg taaagatgac tcgttagaaa
     5581 aacgcgagaa ctcacttgaa agtaaagaag aagcacttag ttcaaaacag caattaattg
     5641 aagagcgaga aaaagacgtt gaaaaaatga ttgaagatca acaaaaagaa ctagaaaaaa
     5701 ttgctggttt atcaaaagag gacgcacgag atgtcatcat gaaatcgact gaagaagagc
     5761 tgaatcatga gttaactcta atggtaaaag aatctgaaca gcgtgccaaa gaagaagcag
     5821 atcgcaaagc gaaaaactta ttatcattag cgattcaacg ctgcgcagca gatcaagttt
     5881 cagaaacgac tgtttcagtc gttacccttc cgaatgatga aatgaaaggt cgtatcattg
     5941 gacgagaagg tcgtaatatt cgaacgcttg agacgctgac tggtatcgat ttaatcatcg
     6001 atgatacacc ggaagcagtt gttctttccg gattcgatcc aatccgaaga gaaatcgcac
     6061 ggatgacctt ggaaaaactg atacaagatg gacgaatcca tccagcgcgt atcgaagaaa
     6121 tggtcgagaa atctcgtaaa gaaatggatg aacgtattcg cgaatacggg gaagaggcag
     6181 cgtttgaagt cggtgctcat acacttcatc cagatctaat caagatcatc ggacgtctgc
     6241 gattcagaac tagttatgga caaaacgtct tgaagcattc ggtcgaggtt gcaaaattag
     6301 ctggtatctt agcagcagag cttggcgaag atatccaatt agcaaaacgt gccgggttgc
     6361 tccatgatat tgggaaagca cttgaccatg agattgaagg atctcacgtt gagatcggtg
     6421 cggaattagc tgccaagtac aaagaaaacc cagtagtaat caacgcgatc gcttctcatc
     6481 atggagatgt ggaagctact tctgttatct ctgtccttgt agctgcagca gatgcgctct
     6541 cagcagcaag acctggtgcg cgaagtgaat cattagaaaa ctacatccgt cgtttaagaa
     6601 gtctggaaaa tatttcgaac agcttcgccg gtgtagaatc aagttacgca gttcaagctg
     6661 gacgcgaagt tcgggtaatg gtcaagccag aagaaatctc ggatttagac gctgttcgtt
     6721 tagtacgtga tattcgtaaa cgaatcgaag aagaactgga ttatcctgga cacatcaaag
     6781 taatcgttat tcgggaaact cgtgcaactg attatgctaa ataataaaaa atacaaaaga
     6841 agagccgcgg caaaagccac agctcttctt tttttgtaag gcacgaaaaa acattgctat
     6901 cattattttt tattcaacat ctcaagatct ttctcgatcg tcgtattgat tcctttgatg
     6961 attgctggga tgaatcctgc ttcttctaag gcaactacac ctgcaacagt tgtaccgcca
     7021 ggagaagaaa cttggtctac aagttcccat ggatttttat cacttgaaag gaatttttga
     7081 caagaaccca taattgcttt tgcagcaatc tcagtggctg tctgtttgga caaaccattc
     7141 agtacaccag tccgtcccat cgcatcgatc agcatgtaaa cgaatgcggg tgagctgcca
     7201 gcaaggccaa tgaatgtact gaaatctgat tctgctaaag gatagacgct gccgattgct
     7261 tcaaaaatca tcgtagcttg ctgaagctgt tcctccgtgg cagcttcatt tttgcaaatc
     7321 gcagaaacgc ctgctcctac gctgacattg acattcggca tcacacgtac gatggaggcc
     7381 tgaggatctt caagcgctgt ttctaaatct attaatgatt ttcctgaagc aatagaaatg
     7441 aataagactt tctggctata ttctcttagt tcttccaata ctttttcaat gatttttggt
     7501 ttcacggcta aaagaacgat atctgattgt gatgcaactt cctgattact tttgcatgca
     7561 tggacgccag tttcagcagc aaatttttgt gcattttcaa aatgtccact ggaaacgaac
     7621 acctcttctt tcgagactgt tccttttttt aataaaccat caataatagc ttgtgccata
     7681 ttccctgtgc cgataaatcc tactttcatg ttatcctctc ctttagcatt aggatatctt
     7741 tctttttcta tcatcgcaag caaagaacag gtctaaagtg gaaaaattaa aataaataag
     7801 ataacattgg ggtatgacac aaaagtatac aagttaggaa tgggaaaatg tgaaaaataa
     7861 acgatacttg atgctgcaat ggataaagga acatagtctg attttaaaac tgattttttt
     7921 cggctccgtg ttagtctttg tggcaaatca agtcgcaaat attgctaatg gcatgagttg
     7981 gcaagatatt tggcaaagga tgggacaaca aagccgcatg acgatcctgc tcatgattct
     8041 ggctggactg ctgggtgtca ctccgatgtt gttgtatgat tgggtgacga tcagagtctt
     8101 agaaaaacaa ggaaaaccaa aaatggaaag aaaagagttt cttcttgctg cgtgggtaac
     8161 aaacacgatc aacaatctcg ccggttttgg tggtgtggta ggagcaagtc tgcgggctag
     8221 tttttatgga aagcagacaa atagaaaaat ggttttagcc actgtatcaa aagtcgcact
     8281 atttatgctg actggtttgt ctgtatggtc gtttcttaca tttattgatg tcttttttat
     8341 tcagtcagaa agtatttttc gaagctattg gatctggctt ttcggcggta gttttttagc
     8401 accagtcatt ttcttttttg cttatctaaa gagaaagaca ttgttcttcg agttttatcc
     8461 aaaaggcttg cttggtctgc tactggcatc ctttggacaa tggacgggag cccttgcggt
     8521 atttttgatc atcggcaagt tgatgagaat agatatttcg atgctttcga tctatcctat
     8581 gttcatgatt gctacattga taggaatgct gacgatggtc ccaggtggaa tggggacttt
     8641 tgacgttttg atgatcttag gaatgagtca attgtctgtt cggcaagatg tagcagtagt
     8701 ttggctatta tattatcggt tgttttatta tgttctacct tttatgacgg gacttctatt
     8761 gttcatcaca catacaggag tcaaaatcaa tcgattttta gataacattc cgcgactgtt
     8821 tttccaaaag gttgcgcata tcgtgctcgt aagtgctgtc tattttgcgg ggatcatgat
     8881 ggtacttcta tcaacaatca ctaatctgtc tgcaatcagt cggctatttc aagtattatt
     8941 gccgttctca ttcaattttt tagatcagac agtgaatcta ttggttggct ttttattact
     9001 cggccttgct agagcacttt cagtaaaagt caaaaaggca tttctgccta cgattgcttt
     9061 actatctttt ggtattttaa atacaatcac acgtacgtta tcttggcagt tgatattggt
     9121 atatgtcttg attctgttag ctgtgtggtt atccagaaaa gaattttatc gaacaaaatt
     9181 cgtttattct tggggagcta taatctttga tgctagcttg ttcggctttt tgtttatcgt
     9241 ttacgcaatt gcaggctatc acagcggacg ctttttagtc ggtcgaataa caggaaatca
     9301 ttttattttg tttccttcag atgatgtgtg gttctcaggg cttattggtt tgggcatctc
     9361 attgctagct ttagtggcgc tttatcaata tttagctgaa gatactacga agcttggcag
     9421 tgagtacgaa aaagaccgtt ttgaccgttt attggaaaca tacggaggca cagctacaag
     9481 ccatcagctt cgcttgtcag gttacagtta tttttattac caagaagacg accaagatca
     9541 agtcgttttt ggttatcaga tcaaaggaaa caaatgcttt gttttaggag atccagttgg
     9601 caatcctaaa atgatgcggg cagcaacgaa tcagtttatg aaacaagcag attgtttagg
     9661 ctatcagctt gcgttttata agatatcaga aagctacgta gtgatgcttc atgaattggg
     9721 ttttcacttc acgaaagtag gtgaagcagg gattgttgat ctagcaacag aagacttgtc
     9781 tcggctgagt cagcaacttg aatatcaacg tttgatgaaa gaaggatacc attttacttg
     9841 gtacgatcaa ctgccggaag aattacatca agctgtaaat gaagtttcac aaaaatggct
     9901 tcaaggtgca cgagaaaaat acttctctgt gggacgattt gataaatcat atgtattatc
     9961 tagcggtgtc ggtattgtcc gtaaagatca aaaagtcgtt ggcttcatca ccgctcagcc
    10021 gatgacgaag aagcaggcgg gatatgattt gttgcgggtc ttacctaaag aatctgagga
    10081 attagctgac tatctgttag ccaatctgtt tattgtatac gagaaaatgg gctactcgga
    10141 agccaatcta ggcttagctc cattagctaa tgttggagaa acagattttt ctttctttca
    10201 agaaaaagtc atgcatatcg tatataatta tggtaatttt ttctattcct tccagtctgc
    10261 ttatgaaaag aaattaaaat atgtcgatca ttgggaagga cgttattttg cttataggaa
    10321 aggaagtaat ttcggatttg ctaccatgca gctactcagt ctgataggaa aagaaaaagg
    10381 aaatgttccg agtttggcag aagaagtgct cacagaatta taagaatcac tagaacttgt
    10441 ttggattatg aagagaaaat tctgtgaaag ctgttgaaac gatgacaaaa tcaaccacta
    10501 ggcagacata agctatactt ttatttttcc ctatgataga atacttgcga cttagacttt
    10561 tcgaatgggt gagaggatgg aattgattga aaaagcgcca gccaagataa atttaggact
    10621 ggatgtatta tatagacggc aagatgggta tcatgagttg gaaatggtga tgtcgagcgt
    10681 agacttggca gatcatctat tttttgaaga aataacagaa gacaaaatca ttgtagaaac
    10741 aaataaagca tttttaccgg tagatgaacg gaacaatgtt tatcaagctg ctttattgct
    10801 aaaaaataaa tacaatatca aaaccggtgt caagatcttt gttaagaaga atattccagt
    10861 agcagctgga ttaggcggag gaagtacaga ctgtgcagct gctctaagag ggatcaacca
    10921 attatggaat ctgggctgtt cgttagaaga actggcagaa atcggtttgg aagtcggtac
    10981 agatgttcct tactgtgtct acggaagtac ggcatttgtc ggtggcaaag gggaagaaat
    11041 ccaagtatta ccttctatgc cacagtgctg ggttgtgttg gtcaaaccaa gaatgagtgt
    11101 ctctacacgt acagtgttta gagaagtaga tacgcaagcc atccaacatc aagacatcca
    11161 cggacttgct gaagccatcc gagagcaaga ttatcagcag atgatcaaaa aaatgggaaa
    11221 cagtttagaa gatataacgg gaaagcggca tccagtcatc catcaaatca aagagcgtat
    11281 gatcaagtac ggagcagatg ctgcattgat gagtggaagc ggaccaaccg tttttgcctt
    11341 gtgtcaacat tattcaagag cgcagagagt atataatggg ttaaaaggtt tttgtgagga
    11401 agtttattta gtacgaaccc tgcaataaaa tataagaagt aaaataggtg atgaaaaact
    11461 gaggaatcct aaaatggtat tcttcagttt tttattgtag tgaaatggct agatatgagc
    11521 agcaactcat ccattttacg atttaatcaa gaatatacat gcttaaatca gaaattgatg
    11581 gatttctatt gactgtgttt ttttattttg atacattagt tcacgttaat aaatcgtaaa
    11641 attccgattt gttcattgaa aggagcatat aaatgaaaaa atatcttttg acagctacgg
    11701 taatgatagg tgctttactg tttgcagcat gtggaaatac gaacaaggaa gcagacaaaa
    11761 aagaggattt gacgatcgtt acgacttttt atccgatgta tgattttaca aaagagatcg
    11821 taggggatga aggaaacgtg aaacttttga tccctgctgg aacggagccg catgatttcg
    11881 aaccaagtgc aaaagaaaga gcagaaattt ccgatgcaga tgttttcgtg tataatagtt
    11941 cagacatgga attttttgtg gattcgttaa aggattcagt ggatagcaaa cagaccttga
    12001 tgatcgaagc agctaaagga atcgaccgtt tagaatcgca agaagcagac gaacatgaag
    12061 aatcagaaga agggcatggt cattcacatg aatacgatcc acatgtttgg ctcgatccgg
    12121 tattagctat aaaagaagtc agaacgattg ctgaagaatt aggcgaaaaa tatccagata
    12181 aaaaggaaac ttttacgaag aatgccgatg cttatatcaa aaagcttgaa gcactggatc
    12241 aaaaatactc cgatgagcta aaagatgcaa caaaccgcac atttgtcact cagcatgctg
    12301 cttttgctta tttagcgaat caatacgact taaaacaagt agcgatttcg ggcgtttcac
    12361 cagatcagga acccacacca agtcgcttag ctgagctaaa agaatttgtg aagaaaaaca
    12421 acatcaaagt gatttatttt gaagaaaatg cctcttcgaa ggtagctgag acattgtcga
    12481 atgaaacagg agtgaagcta gaagtgttga atcctttaga aagcttgact aatgaacaaa
    12541 tcaaagcagg cgaaaattat atttctgtca tggaaaaaaa tctgaaagct ttaaaagaaa
    12601 gtatcaatta agttttagaa tggaggttgt aaggtgcgtt atatcgaaat atctgatttg
    12661 accttttact atgatgatga acctgtacta gaagatgtct cttatcatgt agatgcgggt
    12721 gaatttgtca ttttaactgg agaaaatgga gcagccaagt caaccttggt gaaagcatcg
    12781 ttaggattac tgaaaccttc aaaaggaatg ataaagattg ctgagaaaaa tcaagaagga
    12841 aaaaaattaa gtatcggtta tattcctcaa caagttgcat ctttcaatgc aggttttcct
    12901 agtacggtga tcgaattagt ccgttcagga agatatccaa gaggccgctg gttcaagcgt
    12961 ctctctgaaa aagatcatca acatgtacag cgtgctttag aagctgtagg gatgtgggat
    13021 atgcgaaatc ggcgaatagg tgaattgtct ggcggacaaa agcagcgaat cagtttagcg
    13081 cgtatttttg ctactgaccc tgacttattt gttcttgatg aaccaacaac aggtatggat
    13141 gaagaatcca gaaatgaatt ttatcgtctt ctccgacata gtgcgcatgt ccatgggaaa
    13201 tcagttctaa tgatcaccca tgatcatgaa gatatcaaac agtatgcgga cagacagatc
    13261 cgactggtga gaaaggagga ttctcaatgg cgttgctttc atatgaattc atgagaagag
    13321 cgtttcttgc cgctatcttc attgccggaa tcgccccgat gctaggtgtg tttttagtga
    13381 tcagaagaca atcgctgatg gctgatacgc tttctcatgt ctctttagca ggggtagcgt
    13441 taggattctt tttgaacctt aatcctacac ttacgaccat gctagtcgtt gtattagcag
    13501 ctgtgatatt ggagtatctg cgaacgatct accgaaccta ttcagaaatc tccattgcca
    13561 ttttaatggc tggtggtttg gcgctagcgt tagtattgat gaatttgagc ggagggaatt
    13621 cagcaacaag tatccaatcc tatctgtttg gttcgatcgt gaccattaca tgggagcaag
    13681 tcatcttttt aggaatctta ttcgttgcca tcggtatatt gttcttttta ttcaaacggc
    13741 cgatgtatgt tctgactttt gatgaagata ccgcccacgt ggatgggcta cccgttcatt
    13801 tgatgtcgat gatgttcaat gtgattactg gtgtggctat tgctgtgatg atcccgattg
    13861 ccggtgcact tttgatatcc gcaatcatgg ttcttcccgc agcaatcggt atgcgattag
    13921 gcaaaagctt caatgtcgtg attgctatca gtgtttgtgt aggatttcta gggatgctgt
    13981 caggactaac tagctcgttt tatctagata cacctccagg agcaacaatc acattagtct
    14041 ttattgcttt gttcctgatt gtaaatatca tcaaacgaat attggttgcc ttcagaagaa
    14101 aaaataacta aattataaag aaaagaagcc ttataaaaac gaaaaatttt ttataggctt
    14161 ctttttattt taataaatac cgaactattt tcttattttt tcataaaatg ctcgaatttt
    14221 tgaagaaaaa cttatataat gtaaaacgat aaaatttctt aaaaggagtt ggaataggtg
    14281 aaagtacgtc gcagtgaacg tctagttgac atgacacagt atttattgaa tcacccgcat
    14341 gaattgattt ccttaacaag ctttgccgaa cgttatgaat ctgctaaatc ttctatcagt
    14401 gaagatttgg gaatcatcaa gaaaacattt aaagaacgag ggtatggaac cttggaaaca
    14461 atcccgggtg ctgcaggtgg tgtattgttc atccctgaaa tttcatatga agaggcgaaa
    14521 aaatatattg aatctctagc agaacgtcta tcagaacaag accgtctgct tccaggagga
    14581 tatgtttatc tctctgattt gttaggagaa cctgaactgc tgcgtcaagt tggaagaatc
    14641 attgcttcta aataccttgg tgaaaaaatc gatgctgtaa tgactgtagc cacaaaaggc
    14701 gtacctattg cccaagctgt gtcctattat ctgaatgcgc cattcgttat tgtacgccgg
    14761 gactcaaaaa taacagaagg atcaacagta agtgtcaact atgtgtctgg atcttctgaa
    14821 agaattgaaa agatggaact gtctaagaga agtttgaaac gtggttcacg tgtccttgta
    14881 gtggatgatt tcatgaaagg cggcggaaca gtcaacggaa tgaagagctt gattgaagaa
    14941 ttcgaatcag aacttgttgg catcactgta tttgccgaat caaaattcaa cggtcatcgc
    15001 gccatcgatg attatacgtc actgctttat gtcaaagatg tagatactca aacgaaaaat
    15061 attacggttg tgccaggaaa ttatttttca gaataagagg ttgtgaaaaa gccgcttagt
    15121 ttcgagtagt aagaaaaatt ttgtgaaaat ggctttccac attttttcat aaatttggct
    15181 taataccgag aaactggctt ttgaacaccg tttatctaga ggttgtgaaa tcagcgtcct
    15241 gtcctgagta ttagctcaat aaacggaaaa attgctcctc atattttgcc gtttattgag
    15301 ctaataccga aggtcagctg attgaaaaac gtttaatgaa gaggttgtaa gcctaccgtt
    15361 taactctaag tcacaagaaa cgatttcaaa aaacagcttt tcatgtttta tgagtagtgt
    15421 gacttgtgac cgaggagttg gcaggagagc accggttacc aaatagatag gcccgttatg
    15481 gtctatctat ttttttatcc agaaaacgct gggataaaag aaaatcactt gttttgttga
    15541 aaaaatgggt atactgggat aaactgaaat cagcaaacta tatttaggag tgacgcagtt
    15601 ggacgcacgt tatgcaatta ttttggcggc agggaaaggt acccgcatga agtcaaaatt
    15661 atataaagtt ttacatcccg tttctggtaa acctatggta gaacatatca ttaatcgggt
    15721 cagtgaaacg aaaccagatg aagtgattac gatcgttgga cacggagcag aacaagttaa
    15781 agcacaacta ggagaacgca gtaaatatgc gttacaagca gaacaattgg gaacaggaca
    15841 tgctgttttg caagctgctt ctttcttgga aggaaaaaaa ggaacaacgt tagttatcag
    15901 tggcgataca ccattattga ctactgaaac gctgaacaat ctttttgaat atcaccaagg
    15961 aaaaaatgca agtgcaacga tcttgacggc acaagcagaa aacccaacag gatatggacg
    16021 aatcatccgt gaccatattg ggatcgtaga aaaaatcgtg gaacaaaaag atgcaacacc
    16081 agaagaagct cttgtacaag aaatcaatac aggaacttac tgttttgaca acgaagcttt
    16141 gttcgatgcg ttaagcaaag ttggaacaaa caatgctcaa ggtgaatact atctaacaga
    16201 tatcatcgaa atcttaaaag aagaaggcca tacagttgct gcttatcaaa cggatgattt
    16261 tgaagaatca atgggagtca atgatcggat cgcgttagct aaagcaaatg agatcatgag
    16321 aaagcgaatt aaccagatgc atatggtcaa tggcgtaagc tttgtcgatt cagcaacgac
    16381 atacatcgat gcaggcgtag aaatcggtcc agacacattg atcgaagcag gtgtacaaat
    16441 ccaaggaaat acagtcattg gttctgattg tgtcatcggg tcacattcaa aaatcgtaga
    16501 cagtaggatt gaggatcacg tcgtcattga aaactcagtc attgaaagca gccatgtcaa
    16561 gaaacatgca gatgtgggac cttatgcaca tttacgacca aaagctgaaa ttggcgaaaa
    16621 cgtccatatc ggaaactttg tggaagtgaa aaatgctcaa atcggcaaag gaactaaagt
    16681 cggtcacttg acttatgttg gtgatgctac gctgggagaa gaaatcaatg tcggatgtgg
    16741 tgttgtcttc gtcaactatg atggaaagaa caaacatcat acgactgtcg gcgaccacag
    16801 ctttattggt tctagcacga atatcatcgg acctgtcgag gtagctaaaa acagttcgat
    16861 tgctgccggg tcaacaatta cagacaatat tccagaatat gcgctggcaa ttgcgagagc
    16921 aagacaagtc aataaagaag gatatgcaaa aaaactccct tatctaaact aagacttttg
    16981 caaaatcact tgatttattt aaaaaatatg actactattt attaggtaga attctagagt
    17041 tgtgaaaaag aaagtggagg tccccatgtc aaaacattac tttgacccaa gattaaaaat
    17101 ttttgcgtta aattccaatc gcccattagc agagaaaatt gcagcagcag tcggcgtcga
    17161 gttaggaaaa tcatctgtta ctcaatttag tgatggagaa attcaagtaa acattgaaga
    17221 aagtattcgt ggatcgcatg tctatgttat tcaatcaacg agcagcccag tcaatgataa
    17281 cttgatggag ttgctgatca tgattgacgc actgaaacga gcaagtgcaa aaacaatcaa
    17341 cgtagtcatg ccttattatg gctatgcgcg tcaagaccgt aaagcacgtg cacgggaacc
    17401 aatcacagcg aaattagtag caaacatgat tcaaaaagct ggtgcaacaa gaatgctgac
    17461 tttggacctt catgcagtcc aaatccaagg tttctttgat attccagtcg atcatttgat
    17521 gggtgcgcca cttattgcca attatttctt agaaaaagga atcaaaggcg atgatgttgt
    17581 cgttgtttca ccagaccatg gtggtgtcac acgggcacgt aaattagcgg aatttttgaa
    17641 atctccgatt gcgatcatag acaaacgccg tccaaaagca aatgttgcag aagtaatgaa
    17701 tatcattgga caagtcaaag gcaaaacttg tgtactgatc gatgatatga tcgatacagc
    17761 aggtacgatc acattggctg ccaatgcttt gaaagaagct ggtgcaacta gtgtgtatgc
    17821 ttcatgtaca catccagtat tatccggccc tgctttacaa agaatagaag attcagctat
    17881 cgagcgtcta gttgttacag actcgatcta tcttccagaa gatcgtaaaa tcgacaaaat
    17941 cgatgaagta agtgttggcg gactaatggg agatgcaatc aaacgtatcc atgaaaacaa
    18001 accagttagt ccattgtttg aaacaaaaaa taaataataa ataaactcac aaaggggagt
    18061 ccaattgggc tgagattgaa tctatttcta aacccttcgt acctgtatcg gttatgcgag
    18121 cgtaggaatt gtgaataaac agctgtttga ctattttcga aagctgcttc ctcctttgtg
    18181 acttttttat tcaaaggagg aagttttttt atgggaaaaa atagagtttg ggtagaagga
    18241 acgattgttg cagcgttagc gatggtgttg tcgttgattc cgatccagat cggcagcagt
    18301 ttttctattt cattagggca gattccattg actctttttg cgttaagaag agggacaaag
    18361 gcagggatgc tggcaggttt catttggggc atcctacatt ttccgttggg acaagtttat
    18421 tatttaagtg tcgttcaagt gttgattgag tacccgattg cctatacttt tgcaggcgca
    18481 gcagggctca tggcttcaaa tgtccaaaaa ggctatgtag acgataatac gaaacaaatt
    18541 agaaaaagca tcgtgtttgg tgcattgatt ggcgcacttt ccagatattt ttggcatttt
    18601 attgcaggag tggttttctg gggagcgtat gcactatggg ggatgaatcc ttggctcttt
    18661 tctttcgtaa tgaatggtgc cagcgggttg agtacagcta ttgtaacgat catcgtttta
    18721 ttagggatcc aacaaacgac tccttcgatt tttgtgccag ataaacgaca ggagctgtaa
    18781 ataaaaaaga agcaagaaac ttgagacaga atcatgtcct agtttcttgt ttccttttta
    18841 ttttttgata agaaatagat ctacttcaat ggcagataag aaagatcggc atcttctacc
    18901 aattgccagt gtcgatcggt gacagagtag attaaagtag cgatgatttc ctcttctcga
    18961 taccgggtgc cttttaattt ccaaatataa tcttcaatca catagattgg gatacgatat
    19021 ttaccatcga tcaaaccaac ttgatcaaaa gaaaaaatga tcccttcctg catcaaatta
    19081 cgcatttctt tcaataattc cacactgatt ttttcaacag gtgtttcttt tttgaaaaaa
    19141 gattttttta cattgatctt atctgacaag atcgaactta atttactagc aaaaatccta
    19201 taaaattcat ctaaatagcg ataataaatc cgattcagct gatcattaag atcgaagtat
    19261 acaaaattat tctgtagttt ataaaaaaac ggcgaatgaa gatgtgtttt catatgacca
    19321 aaatataata attctgaaat ttctaaaggt gtcaattctt tcagcatcgt taaatcagta
    19381 aagtcgatcc acttcaattc atcgaatcca cgcctgttac gagaagagga aaagaaacgc
    19441 tgaatatttt ctgtaccacg gattactttc agccctgtat gggattcata ttctccagca
    19501 gaagcagatg gatcaagcaa gagcaagttt tgaggaaaat gcacgatact gtttgcaaag
    19561 tcagtagcag tcagcccttt tgttaaaaca gcattacttg tggtatcaat atgtacataa
    19621 actgactcag tcatcatgtt cctcctctta tttcctctta atctctttta ttttacaaca
    19681 tttgcattaa aaaagcttgt ttcttttatt ttaaaattcc attacaaaat cacgttttca
    19741 ttgaaaacac tctcattttc aattaatttt tgtgattttt gtattttttt atttaaagga
    19801 ggttttatga attgttttct tatggaatct acaaagtcga atacgagact catgaccttt
    19861 ttatgacgta ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta
    19921 cttttcacag aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt
    19981 ttttcaaagt catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc
    20041 ttttaaagca gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga
    20101 tgttattttt aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg
    20161 gtatatggaa gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc
    20221 tcctctatca gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca
    20281 attaaataaa aagctggtct atcatacagc acttgtttta ggtagaggag gagcgcacgg
    20341 tgcttatcag attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat
    20401 cacaggtacc tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa
    20461 agctgtgaat ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc
    20521 tgcagttgaa gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc
    20581 actaattgaa ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga
    20641 tttgtcctta ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc
    20701 agaactagcg gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg
    20761 gatactcgcc tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa
    20821 atatgcagac ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc
    20881 aacagaggca tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc
    20941 agacattttt atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt
    21001 tgatagccaa aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt
    21061 aggctattac tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg
    21121 gcgaggattt ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag
    21181 cataaaattt tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg
    21241 cggattggcg atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta
    21301 tcaagtagac atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga
    21361 tttattggca caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca
    21421 gcaaaaaata caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa
    21481 acgcaaggat cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct
    21541 atatttatat tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta
    21601 gaagaaatcg ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc
    21661 agctggaatg gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa
    21721 atggggaaag gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa
    21781 aatatgtaat gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat
    21841 ccgcgaaaca aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct
    21901 agatgttcaa atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct
    21961 tattaaacag cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg
    22021 caaaaacgaa aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt
    22081 gacagacgga attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc
    22141 ggaacgcttc gtgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat
    22201 ggaagaattg gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc
    22261 gagtcgagaa aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt
    22321 caaagaagta ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat
    22381 aaggtatcaa aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt
    22441 gacggagcta gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc
    22501 gatccatgta caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt
    22561 agatttcctg atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc
    22621 aatcgaaatt aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca
    22681 agtacaaaat attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc
    22741 cacgaattaa gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt
    22801 gacgtatgga aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac
    22861 gacggtgctt acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta
    22921 gaagatccga cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact
    22981 ttgtccaata tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataaacagaa
    23041 ttggatttct tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt
    23101 cgtgtcacaa tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt
    23161 aagccaaatt tatgcaaaaa tgtgagaaac tattttcaca aaatttttct tcatactcga
    23221 aactaatcgg cttcttcacg acctcttata cacggcgttc aaaagcttgc atctgcgata
    23281 ataaccccaa ccaaacgaaa aacatgagaa gctgtttatg tttggctgtt cttattactt
    23341 gatgcaggac agctttttta cgacctcttg gtacagggta gcttttttac caatttctag
    23401 ctataattaa agataacctt gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac
    23461 aaaaagacta gttgaaagga cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct
    23521 ggtccatccg gtgtaggaaa aggcacagta cgaaaagcaa tttttgagag tgaggataat
    23581 gattttcagt attcgatctc aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc
    23641 gttgattatt tcttccgaag caaagaagag tttgaatcaa tgatcgaagc tggagagatg
    23701 ctggaatacg cagaatatgt cgggaattat tatggaactc ctttgtcata tgtcaacaag
    23761 acattggatg aaggaaaaga tgttttcttg gagattgaag tccaaggagc aaaacaagtc
    23821 aaagaaaaag tgcccgatgg tgtattcatc tttttgactc cgccagattt agcggaatta
    23881 aaatcacgga tcgtaggccg aggaacagat gcagatgaag taatcgaaaa acgaatgaaa
    23941 gttgcaaaag aagaaatcga aatgatggct ctttacgact atgctgttgt aaatgatgaa
    24001 gtgccgctag cagttgaacg aatcaaaaac attattgcca gtgaacattt tcgagtagat
    24061 cgcgtaatcg gtaaatatat taaaatgtta aaggagatgt agtcttatga tgttaaaacc
    24121 ttcaattgac tcactattag atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag
    24181 taaacgcgcg catgaattgg atgcaagtgc acagcctact ctagattctt ttgattcagt
    24241 aaaaagcgtc ggtcaagctt tagaagaaat cgatgcagga aatgtagtca atgatccaca
    24301 tcctgaatta aaacgtgaac gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga
    24361 gcgtgaacaa cacgaattgg aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa
    24421 aatagagcga acatctaaaa agaaaagcaa acccagctgt ttccttttca aacattcgcc
    24481 acttattgtc tggtagaaaa ggctgtgtct gaactttttg acacagttct tttttttctt
    24541 atctgctgtt aattttttgt taggatttgg cagaagataa gacaaatcca tcgatctcta
    24601 gtaaaatagg caaggaactt tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg
    24661 aaccatgaca ttaacagctg aagtcattgt ggacgtacct actatgcaga ccgatcagcc
    24721 attcacttat ttggttccct ctgaaatgga aacagctttg caagtcggga tgcgagtaga
    24781 agtccctttt ggtaatggca atcgtcatgt ccaaggattt gttatggcta tccaaaaatc
    24841 agaagaatca gctaatccat ctttgaaggc agttattcgt ttgctagacc ttgctccggt
    24901 ggtcaatgaa gaattacttt cattagctga ttatatgaaa aaaattactt acgcattcaa
    24961 aatcacttgt ctacaaacga tgcttccgag tgtgatgaaa gcagaatatg ataaattgat
    25021 ctatcctttg gcagacacac cagaagttca ggtattgttt gatcagcgag aagtgatttc
    25081 ctggaaggaa gcagaagaag aaggcagcct ttctcaattg attcgctggc gccaagagca
    25141 attagtagat atcaaatatg aagtccatac acgaaataaa gtaaaaacga ttcgtttggt
    25201 ccgttctttg ttaactgaaa agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc
    25261 caagaaacaa aaagaacttc ttttgtgttt atctgaactg agccaagaag aaccaattgc
    25321 ttatttcaaa aataaaggaa tcagtactgc tgtcttgaat caaggaaaag aaaagggctg
    25381 gctggagttt atagaatcag aacgttatcg cgacccttat aaagaccgag tatttgacca
    25441 aacgacggca ttggaactga atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc
    25501 aggtcagcag caaaaagatc aggtcttttt gctggaggga atcactggca gcgggaaaac
    25561 agaagtatat cttcaggcaa tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt
    25621 agtgcctgaa atcgcattga ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga
    25681 atcagttgcg gtcttacata gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa
    25741 gattgagcgg gaagaagcgc aagtagtagt aggcgcacga tctgctattt ttgccccgct
    25801 gaaaaatatc gggctgatca ttatcgatga agaacatgaa tcaagttaca aacaagatga
    25861 gacaccgcgt tatcatgcga gagatctagc aatttggcgc agtaaatatc atcattgccc
    25921 aatagttcta ggaagcgcga cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt
    25981 ttatcagtta ttgcagttaa accaccgagc caaagcagct gcacagcttc cagctattga
    26041 gatcgtagat atgcgtgaag agtttcagaa tcatcggacc tctacttttt ctgctaattt
    26101 gcaggaaaaa atacaaaatc gattagacaa aaaagaacaa actgtcctac tgctcaatcg
    26161 tcgaggatat tcgtcttttg tgatgtgtag ggactgtgga tttgttcttc catgtcctaa
    26221 ttgcgatatc tctcttacac tgcatatgga tacacgttca atgcgctgcc attattgcgg
    26281 acatgaagaa cctattccca atcgctgtcc taattgtggc ggcaataaaa tccgctacta
    26341 tggaacaggt actcaaaaag tagaagagga actccgagaa ctttatccgc aagctaggat
    26401 tttgcgtatg gatgtagata ccactcgaag aaaaggggca cacgaacaga ttttacaaaa
    26461 attcggtgca aaagaagcag atatattatt agggacacaa atgattgcta aaggtttgga
    26521 ctttccagaa gtgacattag ttggggtgtt gaatgctgat acctccctga atttaccgga
    26581 ttttcgttct agtgagcata cattccaatt gcttacccaa gtttcaggac gagcagggcg
    26641 agcggaaaaa gcaggcgaag tcgtgatcca aacattcaat cctcaacatt acgccataga
    26701 attggctaaa aaacaaaatt atgagcagtt ttatcagcag gaaatgcatg tacggcatcg
    26761 tggaggctat cctccttatt actttactgt gaaaataacc gccagccatc ctgaagaaca
    26821 agtagcggca aagaaaatct ttcagattgc caatcagcta aaagaagtgt taagccctca
    26881 aagcttgcta ttagggccaa cgcccagcat gattttgaga gtgaaaaacc gatattatta
    26941 tcaactaatc atcaaatata aacatgaacc gaatcttcct catgttttgg atgaaatttt
    27001 gaatggaagc cagaaagaac agcgacaagg tctttttgtg gcaattgaca acgaaccatt
    27061 aaactttatt taagaaaggg ccaaattatg cgctatccta tattgatcca ccctaatgat
    27121 aaattaaaaa gaactgctca gccaatcgat gtaatcacgg acgagacaat cgctttatta
    27181 gacaatttat atgagacaat gattgcgaat gacggaatag ggatcgctgc tcctcaagta
    27241 ggacaaaaca agagaattgc tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt
    27301 aacccagaaa tcatcgaagc aaaaggtgaa agccttgacg tagaaggttg tctcagtatt
    27361 ccgcatgtct atggaacggt caagcgagca gatgaagtga ccgtgcgtta ttatgatcga
    27421 gacggagaag agatagaagt gacggctttt ggctatttag cccgtgcctt ccagcatgaa
    27481 atcgatcact tagacggtat cttgttcatt gagaaaatga tacagcagat cccggaagaa
    27541 gaacttgaag aatatatgga ggaacatctt gatgactaag ctagtattta tgggcacacc
    27601 tgctttttct gtgccgattc ttgaagggct gatagaatca ggttacgaga tccaggcagt
    27661 ggtaacacag cccgaccgtc cagttgggcg gaaacgagta attacgccga caccggtaaa
    27721 aaaagcggct ctgaagcacg gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc
    27781 agaaatggaa gaaatcattg aattggtgcc tgatgtgatc gtcactgcgg catttggcca
    27841 attcttgcct gaaaagttat tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc
    27901 tttacttcct aaatatcggg gaggtgcgcc tgttcattat gccatcatga atggggagaa
    27961 agaaactggt gtaacgatca tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc
    28021 gcaagaaagc ataccaatca ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag
    28081 ccttttagga agaaagctat tgctagaaac attgcccaat attttggatg gacaaaaacc
    28141 agtacctcaa gacgagtcag aagcaacttt ttcgccaaac atcacacggg aacaagaagc
    28201 aatcgattgg cataaaactg cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg
    28261 gccaattgca ttcacgacct atgagggcat acgctggaaa ttattatcgg ttgaagtatt
    28321 ggcagaaact acagacaaag cacctggtac gatcatccgg aaagacaaaa aaaatctatg
    28381 gattgcttgc ggtaaacaga ccgtcttagc catcaatgaa ttacagccag ctggtaaagg
    28441 aaaacaggcc gtacatgatt tccttaacgg aagcggtcag caagttcaga ttggtcagca
    28501 ggtgaaataa tgaaaaaaat ccctaaaaaa atgaaaaaat cagtacgata tatggcatta
    28561 ctgacattag agcgagtgaa caagggtggc gcctactcga atcttttgtt gaatgagatg
    28621 atcaaacaaa gcgagctaaa tcaaaaagat gtgggtttat ttacagaatt gatctatggt
    28681 acgattagcc atcagcgact attggaattt tacgttgcac cattgatcgc taaagcaaaa
    28741 aaagtggatg actgggtcaa gatgcttctt tatctttctg tataccaatt agaattttta
    28801 gataaagtcc ctgcacatgc tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga
    28861 aatcctggga cagggaaatt tgtaaatggt gtcttgagga attatcagcg tcaaggagca
    28921 ccagatctgc aaggcattgc tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct
    28981 ctatggatga cgaaacgact ggtggatcaa atcggatacg aagaaactga aaagctcggt
    29041 ttatcgctat atcagcctag ccatgctagt gcccgagtag atacacgccg actttccaga
    29101 gatcaggcca tcactgtttt gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt
    29161 ccatacggta tagtagcgaa aaaagggcat ttagcttcta gctcattgtt ccacgatggc
    29221 gtcatgacga tccaagatga aagttcgatg cttgtagcgc cgtctatgca aatcgagcct
    29281 gaacatcatg tcttagatgc ctgtgcagca cccggaggta agacgacgca tattgcttct
    29341 tttttagatc cgtcatgcgg tggagaagtg gtcgctttag atgtacacga acacaaagtc
    29401 aaactcattg aagataatgc agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag
    29461 atggatgcta gagaagtcag taaacagttc gaagcagagc agtttgatcg gattttagta
    29521 gacgcgcctt gttcaggtct tgggctgctt cggcgaaaac cagatattcg ttataaaaaa
    29581 cagccggatg aattagcgaa tttaccaaaa atccaattgg aaattttaga aagctgcgca
    29641 ccaactttaa agtcttccgg tattctgact tatagcactt gtacgatctt aaaagaagaa
    29701 aaccaagacg ttgtttctgc tttcttggaa agacaccctg attttgagag gattgatgtt
    29761 ttgaccaatc ctatgctaca atcatcaatc gaagagaaaa tgttgacttt gtatccgcat
    29821 cagttttaca cagatggatt tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa
    29881 atgcagattg aatatcaatc agatgtagga agacgacgta acacaaatca agactatgcc
    29941 agtgtattta ccagccgttc aggtctcaag cttgctgtat tagcagacgg gatgggtggt
    30001 catcgtgccg gagacattgc cagtcagatg gcggtaacga atctaggcga agcttgggaa
    30061 aaacaagaag ctgttgacga tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa
    30121 gaaaatatgc tgatttatca gcgtggacaa gaacagccgg aatataacgg tatgggaaca
    30181 acaatcgtag cggccgctct gtcagatgac tggttcacga ttgccaatgt tggggatagc
    30241 agagcttatt tgattcgtga agataaaatc atgcaattga cggaggatca ttctttagtc
    30301 aatgaactag tcaaatcagg agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa
    30361 aatattttaa cacgttctgt cggcatgcct ggaacagtcg aagtagatgt ttctactcat
    30421 ctttggcaac taaaagatcg actgctgtta tgttcagatg gcttgacgaa catgctgtct
    30481 gaagaagaaa taggaacgat catcaatcaa gaaggcccat tgtctcataa agtaactgag
    30541 ctgattgatc gtgccaatga agcaggcgga gcggataata tcaccgttct tcttatcgaa
    30601 tataaggagg acagtgtatg atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca
    30661 ctggaaatat cggtagcggt ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg
    30721 atcgtgatgt agcagttaaa gtcttacgat ttgactttca aaatgaccag actgcgattc
    30781 gacgatttca gagagaagct ttagcagcaa cagaactcgt tcatccaaac attgttagtg
    30841 tttacgatgt tggtgaagaa gacaacatgc agtatctggt gatggaatac gtcaaaggaa
    30901 tggacttgaa acgatatatc caaacacatt atcctgttcc ttatgaaaca gcagtaaata
    30961 ttatgcagca gatcctttct gctatttctt tagctcataa ccatcaaatc atccaccgag
    31021 atttgaaacc ccaaaacgta ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg
    31081 ggattgctat cgctttatct gaaacatcga tcacgcagac gaatacgatg ctaggatctg
    31141 ttcattatct gtcccctgag caggccagag gaagtatggc aacgaagcag tcagatattt
    31201 atgctttagg gatcatcctt tatgagatgt tgacgggtag cgtaccgttt gatggtgaat
    31261 ctgctgtaac aattgcttta aaacattttc aagatgatct tccatctata aaagcacttg
    31321 atccaaatgt gccacaagca ctggaaaacg tgattttacg ggcaacggct aaagaacctg
    31381 cagaccgtta taagtcagcc gaggaaatgt ctgatgatct ttctacagta ttatcaccag
    31441 ctcgtgcaaa cgaagaaaaa tggcagcctc atgtgatgga taatgaaaca aaagttatca
    31501 caccccttac ggaagatgca ccaatgcctg attcattcaa atctatgcct ctgccaaaag
    31561 acaagccaga aaacgaaacc ttaccagagg aggaagaagt tctgcctgat cctaaaaaga
    31621 aaggacagaa aaaatggtgg ctgatcctat tagctattct tgctgtttta ggaatcggaa
    31681 cagctgtatt ctttgcttct ggcgggcgag gagaagttgt tgttcctgat gtttctgatc
    31741 tttctgaagc ttctgctaga gaaacactcg caaaagcgaa attagaagta gcaacaaaaa
    31801 cagaagaaat tgcagatgac aaaatcgaag aaggtaatgt agtgaagaca gacccggcag
    31861 cgggaacgac agtcaagcaa aaacgcgaag tcactctgta tattagtagc ggcacgaaaa
    31921 aaatcaaatt agatgattac agtggtaaaa gttatgaaga tgccgaagca agtttgaaag
    31981 aattagggtt ttctgctgac ttgattcaaa aagaagagga attcagttca gaggtagaaa
    32041 gcggaatgat catttcgcaa tctccatcag aagatacaga agtcgatcct aaaacagaca
    32101 cgattacttt cacagtcagc aaaggaccag aaacagtgac tttgagtagt catgtaggag
    32161 aaaactacga aactgttcgt aattggcttg ttacacaagg attcaatatc aataatatta
    32221 gtgctactta tgactatagc gacactgtac catcaggttc gatcatcagt caggatccag
    32281 cctcgggtga agttgtagca gaagaaactt acatcaattt tgtcgtaagc caaggaacag
    32341 agcctaaatt gaatgatatc tccggctata caaaaagcga agcacaaagt tatttagcta
    32401 gtgtcggtgc agaatatatc gggcatgaga cttccgaata ttcaaacacg gtggataaag
    32461 ataaagtgat tcgtacagta ccaggggcag gaacgacaat taccaaagga atggttgtga
    32521 acgtaattta ctctaaagga ccagatccag cgtcttctag ttctgaaaaa gactcttcag
    32581 acagttctag cagctcaagt agctccagta gcacaagcaa ctcttcaaaa gaagaatctt
    32641 ccagctccac aagttcatca gctagcacct cagaaagttc aaaataagtg ctgaataaaa
    32701 tatatcagaa gggaatgcga cattgatttg ttgcattctc ttttttgtgt ttctatctct
    32761 tttgatcaat tttcaagcca tatactttga gaagtttgca tggaataaca cctactttcc
    32821 tcttcgtatg gtaaaataaa caaaaacgga ggtggcactt atcaaaggac aaatcagaaa
    32881 agcaatcagc ggtttttact atgtatacgc aaacggagag acctatcaaa caagaggaag
    32941 aggaaatttc cggaatcgga aaatcacgcc gcttgtcgga gatcaggtga tctttgagag
    33001 tgaaaatcaa acagacggct atcttctgga aatcttgccg agaaaaaatc aattagttcg
    33061 cccaccagtc acaaatgttg atcttggaat cattgtcaca agtatcgtag agcctgcatt
    33121 ttcttataat ctacttgacc gctttctagt taccttggaa tacgagtcga tcgaaccgat
    33181 tatttttttg acaaagaccg acttgtcaga cgacccagaa aaaatcgaag agatcaagca
    33241 aacctatact aaaataggct atccggtgat tgtacccaat catacgggtg atacgacaga
    33301 actgatccgt tacttcccag aacggctatc ggtctttatg ggtcagtctg gagcaggaaa
    33361 atcgacgcta ctgaatcaaa tatcaccgga actgaattta gcaacaggag agatttctga
    33421 tgcacttgga agaggacggc atacaacacg tcatgtggaa ctgattccta tctatggcgg
    33481 gttagtagca gatacaccag gatttagctc gattgacttt ttgacaattg aagcaggaga
    33541 actgccgaga caatttcctg aatttgtaga agcttctgtc cattgcaaat ttagagagtg
    33601 catgcatgct aaagaacctg gctgcgaagt aaaacataga gtagaaacag gtggtatcgc
    33661 acagacacgt tacgacaatt atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat
    33721 gtatgcaaaa aaagaaggta aacgaaaata ggaggaaaca agaatgaaga tagcaccatc
    33781 cattttgagt gcagattttg ccaatttaca aagagacatt gagttagtgg aaaaaggagg
    33841 cgcagattac atccacgtag atgtaatgga tggccgattt gtcccaaata tcacattagg
    33901 gccaaatatt gttcaggcaa tccgtcctgt tactaaatta ccgttagacg tccatctgat
    33961 gatcgttgat ccagaaaact acattccagc atttgcaaaa gctggagcag atatcattac
    34021 tgtccacgtc gaagcaactc ctcatattca tcgggcactt caaatgacaa aaagcttagg
    34081 agttaaagct ggagtagtta tcaatccagg aacaccagtc tccatgatca aacatgtttt
    34141 gcctttggct gatcaagtac tagtcatgac tgtcaatcct gggttcggcg gacaaagctt
    34201 tattgaagaa acagtagaaa aaattacgga actcgctgat ctacgtagag agaacaattg
    34261 gacttatgca atcgaagtag atggcggaat cgttccagaa acagcaaaaa tctgtaaaga
    34321 tgcaggggcg gatgtcttcg ttgcaggatc atatatctat ggagccgaaa atccagtcgg
    34381 acaaatcgaa cgattaaaag aggcattaga ctaaatgaaa attttattag ctgctggagg
    34441 taatccgcaa aactggccgc agtctctgct gtccaaatac gatgcctatg tcgggataga
    34501 ccgtggtgcg ctgtacctgc ttcaagcagg ctacccagta gatctggcag tgggtgattt
    34561 tgattcattg tcgaacgaag agcagaagga agtatttgca aaagccaaag agtatacccg
    34621 ttcccctgca gaaaaagacg atacagatac acagttaggt atcttaaaaa catttgaacg
    34681 ttacccgcaa gcggaagtca ctttgattgg agctacgggc ggaagattgg atcatctttt
    34741 agccaatctc tggttaggta tggaacctcg cttcaaaccc tttgcccatc agcttattat
    34801 cagagattgt caaaattatt tgtcttatta tttgcctgga gagcatatag taagacagat
    34861 accagatatg aagtatttag cttattgctg cttgactccc gtctcgaatc ttacattaaa
    34921 aggcagcaag tatgtattgg aaaataaaga agtagaatat ccgacatctt atgcaagtaa
    34981 cgaatttctt tcagataccg cagaattttc tttttcagat ggtatgattg cagttattca
    35041 aagcaaagat tgatccataa acaaataaaa aaatttgtct ttcaggctaa aaatgacaga
    35101 atctattatt tgctaaaaaa aagcttaaga ggagcaactt accttttgtt tcacggcatt
    35161 tagtgataaa attgaacaca agagtctaat taattcgagg tgtctttgtg aagaagttca
    35221 atcctaataa aaatattatt attaccttgg ttattgttat catcgtggtg acgatactaa
    35281 gtgtgaccgc tgcgcagcga gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta
    35341 atgacagtgt tgcaatggtt gataaaacaa tctctgcccc agtacggtgg gtacgaaatg
    35401 gagtagattc tgtccataat cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa
    35461 aaatcgatag ctatgatgac gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag
    35521 cgttgaaaaa agaacttgag ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa
    35581 atgtcattac tcgttcgcct gacagttggc aggacatgct gatcgtagac aagggttcta
    35641 aagacggtat cgaagcaaat atggcagtca tgtcgcaaaa aggattgatt ggccgagtga
    35701 tcgaggtcaa tacggcttcg tctaaaatcg aattactgtc atcctctaat gaaagctcca
    35761 atcattttcc agtacgggta tcttcggcta atggcgaagc gtttggtttg cttaaaaact
    35821 atgatgaaaa gctccatgcc ttagtggtga cccaattaac tggtgatacg gatatcaaag
    35881 aaggggatgt tgtccagaca tccggtcttg gagggaattc tccagctaac ttgccgatcg
    35941 gtacggttat taaaacgaaa ccagatagtt atgggctgga tcgggaagtt tatgtgaaac
    36001 cttatgcaga aatgtatgac gtgtcagttg tgacgattgt ccaacggcta gcggaggatt
    36061 agtccatgtt gaagaaagaa acaatgaagt actatctgcc aatcgttttg ttctttttga
    36121 tgttgataga tggtcattta acaagaatgc taggggagtg gtcgaaaggc acctatatgt
    36181 caaatgccca ctttctgata ttggcattat tatgttgcag tatggcgttt gaaaaacgtt
    36241 atttactgat taccacgatt gttctcgggg ctatctatga cgcttactat attggcgtta
    36301 tcggtatcta tgcagtagct ctccctttaa ttgtatggtt gatgtatgta atgaaagacg
    36361 ttatccatgt caacatcttt actgaatttt tcagtatgat catctttgtc acgggttatg
    36421 aattgtttac gatggtggtc cagttgattt ttaaattagc agtagtaaat aacacgtatt
    36481 ttattacaag gtttttagga cctacactgc tgttgaacat gattatattt gtattattca
    36541 tttttccctt taagaaatta ttcagtgatg aataatttct ttttttattt ttaggtcgaa
    36601 agcgaaaaag aatcgttgtt atcttaagaa atgctagact taacgcggct ttttcatgat
    36661 cattttctga aacgaacagt tttgtcattt ttaaaagttg ttttgaaaca tttttgtaac
    36721 ataactatta tatcgttgac acactgctat ggtacaattt ttatatcgtt aagaaagact
    36781 taaatatatt tagatattta ttaaattaag ccagtcggag gaatgaagag tgaaaaagag
    36841 tttaatatca gcagtaatgg tatgttcaat gaccttgact gccgtagcat caccgatcgc
    36901 tgccgcggcg gacgattttg attctcagat acaacagcaa gatcaaaaga ttgcagactt
    36961 gaaaaatcaa caagcagatg cacaatcaca aatcgatgcc ttagaaagcc aagtttctga
    37021 aatcaacaca caagcacaag acctattagc aaaacaagat acattgcgtc aagaatctgc
    37081 acaattagta aaagacattg cagatttaca agaacgtatt gaaaaacgtg aagatacaat
    37141 ccaaaaacaa gcacgcgaag cacaagtaag caacacaagc tcaaactaca tcgatgctgt
    37201 attgaatgct gattcattag cagatgctat tggacgcgtt caagcaatga caacgatggt
    37261 aaaagcaaac aacgacttga tggaacaaca aaaacaagat aaaaaagctg tagaagacaa
    37321 aaaagctgaa aacgatgcta aactaaaaga attagctgaa aaccaagctg ctttggaatc
    37381 tcaaaaaggc gatcttttat ctaaacaagc tgatttgaac gtactgaaaa cttcattagc
    37441 tgctgaacaa gcaactgctg aagacaagaa agctgacttg aaccgtcaaa aagcagaagc
    37501 tgaagcagag caagctcgta ttcgtgaaca acaacgtcta gcagaacaag ctcgtcaaca
    37561 agctgcacaa gaaaaagctg aaaaagaagc acgcgaacaa gcagaggctg aagcacaagc
    37621 tactcaagct tcatctactg cacaaagctc agcaacagaa gaatcatcag ctacacaatc
    37681 ttcaatgact gaagaatcat catcagctac acaatcttca gcaacagaag aatcaacaac
    37741 acctgaatct tcaacagaag aatcaacagc gcctgaatct tcagcaacag aagaatcaac
    37801 aacagcgcct gaatcttcag caacagaaga atcaacaaca gtgcctgaat cttcagcaac
    37861 agaagaatca acaacagtgc ctgaatcttc aacaacagaa gaatcaacaa cacctgcgcc
    37921 tacaacacca tcaacagatc aaagtgttga tacaggaaac ggcacaggaa gtagtactcc
    37981 ggctccaacg ccaacaccaa cacctgaaca accaaaacct gtaacacctg ctccagctcc
    38041 ttctggaagt gtgaatggtg cagcaatcgt agcagaagct tacaaatata ttggtactcc
    38101 atatgtttgg ggcggtaaag atccaagtgg ctttgactgc tcagggttca cacgctatgt
    38161 ttacatgcaa gtaactggtc gtgacatcgg tggttggaca gttcctcaag aatcagctgg
    38221 aacaaaaatt tcagtttctc aagcaaaagc tggagacttg ttattctggg ggtcacaagg
    38281 cggaacttac cacgtagcga ttgctttagg cggaggacaa tatatccatg ctcctcaacc
    38341 aggcgaaagc gtaaaagttg gttcagttca atggtttgcc cctgactttg ctgtcagcat
    38401 gtaagaatga t
//
LOCUS       DASJSZ010000018        37624 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.018, whole genome shotgun sequence.
ACCESSION   DASJSZ010000018 DASJSZ010000000
VERSION     DASJSZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.018"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..610)
                     /locus_tag="U4B03_000869"
     CDS             complement(<1..610)
                     /locus_tag="U4B03_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067741.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKL"
     gene            complement(789..1508)
                     /locus_tag="U4B03_000870"
     CDS             complement(789..1508)
                     /locus_tag="U4B03_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2067742.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1639..2790)
                     /locus_tag="U4B03_000871"
     CDS             complement(1639..2790)
                     /locus_tag="U4B03_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEN2067743.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3007..3747)
                     /locus_tag="U4B03_000872"
     CDS             complement(3007..3747)
                     /locus_tag="U4B03_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2067744.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3751..4074)
                     /gene="rsfS"
                     /locus_tag="U4B03_000873"
     CDS             complement(3751..4074)
                     /gene="rsfS"
                     /locus_tag="U4B03_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEN2067745.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4131..4724)
                     /gene="yqeK"
                     /locus_tag="U4B03_000874"
     CDS             complement(4131..4724)
                     /gene="yqeK"
                     /locus_tag="U4B03_000874"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEN2067746.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4708..5358)
                     /locus_tag="U4B03_000875"
     CDS             complement(4708..5358)
                     /locus_tag="U4B03_000875"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEN2067747.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5368..5679)
                     /gene="yhbY"
                     /locus_tag="U4B03_000876"
     CDS             complement(5368..5679)
                     /gene="yhbY"
                     /locus_tag="U4B03_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEN2067748.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5695..6804)
                     /gene="yqeH"
                     /locus_tag="U4B03_000877"
     CDS             complement(5695..6804)
                     /gene="yqeH"
                     /locus_tag="U4B03_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEN2067749.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6801..7328)
                     /locus_tag="U4B03_000878"
     CDS             complement(6801..7328)
                     /locus_tag="U4B03_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEN2067750.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7508..8293)
                     /locus_tag="U4B03_000879"
     CDS             complement(7508..8293)
                     /locus_tag="U4B03_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEN2067751.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8286..9155)
                     /locus_tag="U4B03_000880"
     CDS             complement(8286..9155)
                     /locus_tag="U4B03_000880"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEN2067752.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9156..10535)
                     /locus_tag="U4B03_000881"
     CDS             complement(9156..10535)
                     /locus_tag="U4B03_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEN2067753.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10543..10962)
                     /gene="fabZ"
                     /locus_tag="U4B03_000882"
     CDS             complement(10543..10962)
                     /gene="fabZ"
                     /locus_tag="U4B03_000882"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN2067754.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10987..11463)
                     /gene="accB"
                     /locus_tag="U4B03_000883"
     CDS             complement(10987..11463)
                     /gene="accB"
                     /locus_tag="U4B03_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEN2067755.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11466..12701)
                     /gene="fabF"
                     /locus_tag="U4B03_000884"
     CDS             complement(11466..12701)
                     /gene="fabF"
                     /locus_tag="U4B03_000884"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEN2067756.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12720..13457)
                     /gene="fabG"
                     /locus_tag="U4B03_000885"
     CDS             complement(12720..13457)
                     /gene="fabG"
                     /locus_tag="U4B03_000885"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEN2067757.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13469..14386)
                     /gene="fabD"
                     /locus_tag="U4B03_000886"
     CDS             complement(13469..14386)
                     /gene="fabD"
                     /locus_tag="U4B03_000886"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN2067758.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14493..14717)
                     /locus_tag="U4B03_000887"
     CDS             complement(14493..14717)
                     /locus_tag="U4B03_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN2067759.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14770..15732)
                     /locus_tag="U4B03_000888"
     CDS             complement(14770..15732)
                     /locus_tag="U4B03_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN2067760.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15742..16182)
                     /locus_tag="U4B03_000889"
     CDS             complement(15742..16182)
                     /locus_tag="U4B03_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2067761.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            16488..17453
                     /locus_tag="U4B03_000890"
     CDS             16488..17453
                     /locus_tag="U4B03_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2067762.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(17510..17740)
                     /locus_tag="U4B03_000891"
     CDS             complement(17510..17740)
                     /locus_tag="U4B03_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEN2067763.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17826..18713)
                     /locus_tag="U4B03_000892"
     CDS             complement(17826..18713)
                     /locus_tag="U4B03_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN2067764.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18802..20025)
                     /locus_tag="U4B03_000893"
     CDS             complement(18802..20025)
                     /locus_tag="U4B03_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2067765.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(20042..21088)
                     /locus_tag="U4B03_000894"
     CDS             complement(20042..21088)
                     /locus_tag="U4B03_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2067766.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(21252..22979)
                     /gene="ptsP"
                     /locus_tag="U4B03_000895"
     CDS             complement(21252..22979)
                     /gene="ptsP"
                     /locus_tag="U4B03_000895"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEN2067767.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(22979..23245)
                     /locus_tag="U4B03_000896"
     CDS             complement(22979..23245)
                     /locus_tag="U4B03_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEN2067768.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            23724..25958
                     /locus_tag="U4B03_000897"
     CDS             23724..25958
                     /locus_tag="U4B03_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2067769.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            26106..26426
                     /locus_tag="U4B03_000898"
     CDS             26106..26426
                     /locus_tag="U4B03_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN2067770.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(26631..26855)
                     /locus_tag="U4B03_000899"
     CDS             complement(26631..26855)
                     /locus_tag="U4B03_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEN2067771.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(27053..28633)
                     /locus_tag="U4B03_000900"
     CDS             complement(27053..28633)
                     /locus_tag="U4B03_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEN2067772.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(29034..30410)
                     /locus_tag="U4B03_000901"
     CDS             complement(29034..30410)
                     /locus_tag="U4B03_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN2067773.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(30538..31656)
                     /locus_tag="U4B03_000902"
     CDS             complement(30538..31656)
                     /locus_tag="U4B03_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2067774.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            31798..32232
                     /locus_tag="U4B03_000903"
     CDS             31798..32232
                     /locus_tag="U4B03_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2067775.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(32310..32843)
                     /locus_tag="U4B03_000904"
     CDS             complement(32310..32843)
                     /locus_tag="U4B03_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEN2067776.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(32937..34115)
                     /gene="mutY"
                     /locus_tag="U4B03_000905"
     CDS             complement(32937..34115)
                     /gene="mutY"
                     /locus_tag="U4B03_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEN2067777.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(34108..34905)
                     /gene="recX"
                     /locus_tag="U4B03_000906"
     CDS             complement(34108..34905)
                     /gene="recX"
                     /locus_tag="U4B03_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEN2067778.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            35012..36394
                     /gene="rlmD"
                     /locus_tag="U4B03_000907"
     CDS             35012..36394
                     /gene="rlmD"
                     /locus_tag="U4B03_000907"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN2067779.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(36568..37146)
                     /locus_tag="U4B03_000908"
     CDS             complement(36568..37146)
                     /locus_tag="U4B03_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEN2067780.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            37468..>37624
                     /locus_tag="U4B03_000909"
     CDS             37468..>37624
                     /locus_tag="U4B03_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN2067781.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPM"
BASE COUNT        10673 a         8020 c         6486 g        12445 t
ORIGIN      
        1 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
       61 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
      121 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
      181 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
      241 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
      301 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
      361 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
      421 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
      481 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
      541 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
      601 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
      661 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
      721 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
      781 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
      841 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
      901 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
      961 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
     1021 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
     1081 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
     1141 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
     1201 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
     1261 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
     1321 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
     1381 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
     1441 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
     1501 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
     1561 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
     1621 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
     1681 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
     1741 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
     1801 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
     1861 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
     1921 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
     1981 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
     2041 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
     2101 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
     2161 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
     2221 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
     2281 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
     2341 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
     2401 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
     2461 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
     2521 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
     2581 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
     2641 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
     2701 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
     2761 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
     2821 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
     2881 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
     2941 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
     3001 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
     3061 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
     3121 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
     3181 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
     3241 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
     3301 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
     3361 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
     3421 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
     3481 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
     3541 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
     3601 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
     3661 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
     3721 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
     3781 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
     3841 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
     3901 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
     3961 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
     4021 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
     4081 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
     4141 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
     4201 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
     4261 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
     4321 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
     4381 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
     4441 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
     4501 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
     4561 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
     4621 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
     4681 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
     4741 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
     4801 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
     4861 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
     4921 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
     4981 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
     5041 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
     5101 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
     5161 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
     5221 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
     5281 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
     5341 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
     5401 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
     5461 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
     5521 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
     5581 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
     5641 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
     5701 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
     5761 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
     5821 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
     5881 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
     5941 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
     6001 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
     6061 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
     6121 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
     6181 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
     6241 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
     6301 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
     6361 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
     6421 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
     6481 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
     6541 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
     6601 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct cattataatg
     6661 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
     6721 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
     6781 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
     6841 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
     6901 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
     6961 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
     7021 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
     7081 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
     7141 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
     7201 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
     7261 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
     7321 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
     7381 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
     7441 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
     7501 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
     7561 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
     7621 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
     7681 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
     7741 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
     7801 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
     7861 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
     7921 cctctccttc gccgcgctct tctgctccta caccgcaata agcacctgct gtattgatca
     7981 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
     8041 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
     8101 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
     8161 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
     8221 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
     8281 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
     8341 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
     8401 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
     8461 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
     8521 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
     8581 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
     8641 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
     8701 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
     8761 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
     8821 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
     8881 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
     8941 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
     9001 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
     9061 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
     9121 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
     9181 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
     9241 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
     9301 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
     9361 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
     9421 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
     9481 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
     9541 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
     9601 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
     9661 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
     9721 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
     9781 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
     9841 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
     9901 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
     9961 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    10021 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    10081 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    10141 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    10201 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    10261 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    10321 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    10381 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    10441 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    10501 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    10561 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    10621 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    10681 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    10741 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    10801 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    10861 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    10921 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    10981 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    11041 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    11101 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    11161 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    11221 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    11281 ttcaggtatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    11341 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    11401 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    11461 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    11521 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    11581 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    11641 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    11701 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    11761 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    11821 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    11881 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    11941 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    12001 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    12061 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    12121 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    12181 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    12241 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    12301 ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt
    12361 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    12421 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    12481 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    12541 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    12601 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    12661 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    12721 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    12781 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    12841 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    12901 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    12961 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    13021 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    13081 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    13141 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    13201 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    13261 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    13321 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    13381 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    13441 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    13501 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    13561 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    13621 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    13681 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    13741 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    13801 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    13861 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    13921 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    13981 tcggcgcatt catcactgca accattttcc cgcttccagc tggggcagct tctgtcatat
    14041 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    14101 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    14161 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    14221 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    14281 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    14341 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    14401 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    14461 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    14521 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    14581 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    14641 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    14701 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    14761 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    14821 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    14881 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    14941 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    15001 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    15061 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    15121 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    15181 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    15241 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    15301 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    15361 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    15421 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    15481 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    15541 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    15601 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    15661 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    15721 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    15781 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    15841 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    15901 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    15961 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    16021 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    16081 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    16141 actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat
    16201 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    16261 ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca
    16321 actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct cacatatttt
    16381 gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt
    16441 tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa
    16501 aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg
    16561 tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga
    16621 ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg
    16681 tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc
    16741 gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta catattacca
    16801 ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct
    16861 ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt
    16921 ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg
    16981 aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt
    17041 acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc
    17101 ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt
    17161 ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa
    17221 agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt
    17281 ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa
    17341 aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac
    17401 agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc
    17461 tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta
    17521 agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat
    17581 ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta
    17641 cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct
    17701 ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta
    17761 tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc
    17821 gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct
    17881 tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg
    17941 gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct
    18001 ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa
    18061 taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt
    18121 aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct
    18181 gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat
    18241 gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct
    18301 ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt
    18361 gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt
    18421 ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc
    18481 gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata
    18541 ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa
    18601 ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga
    18661 tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg
    18721 acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt
    18781 ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc
    18841 aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg
    18901 ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc
    18961 taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa
    19021 gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata
    19081 agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc
    19141 ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac
    19201 atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat
    19261 caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg
    19321 ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga
    19381 aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa
    19441 tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt
    19501 aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc
    19561 acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg
    19621 ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg
    19681 gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc
    19741 tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc
    19801 ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg
    19861 cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata
    19921 gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc
    19981 gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa
    20041 actagtcctt gacctgattt acttttttta atttgctaga gactttttca ttcagtacac
    20101 tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt
    20161 gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga
    20221 taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat
    20281 acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga
    20341 tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt
    20401 ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta
    20461 caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg
    20521 tgtattcggg catcttctta gcaatttcta cgaaatcaat gattccttta cgttcgaaaa
    20581 acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact
    20641 tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt
    20701 gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga
    20761 tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct
    20821 ggttcgatct gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa
    20881 ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat
    20941 tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg
    21001 tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt
    21061 cactttcaaa atataataat accttcacct tacgtccccc ttcacttata tagtataaca
    21121 tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta ctgacaaaaa
    21181 cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca caccagcttt
    21241 tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc catcatatcg
    21301 cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa gcgtttcatt
    21361 aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag acccatacct
    21421 actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc tgcccatttt
    21481 ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga tgggttatat
    21541 ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt gtattgaatc
    21601 aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc tgctaagact
    21661 gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac ttccttgcct
    21721 tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg gaattctttg
    21781 agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc acgaagcaat
    21841 gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat acgcaatgca
    21901 cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag ttctttatct
    21961 ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc taatacagct
    22021 ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat atacaggaat
    22081 tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac tagatctttt
    22141 ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac agtttctgca
    22201 ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat
    22261 tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc gttgacagcc
    22321 aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat tgctggaatt
    22381 tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc tgttacaaac
    22441 gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc atgtgcaaca
    22501 acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa taagtgcgct
    22561 aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata agcattatct
    22621 tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc tgattcagca
    22681 tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg atctgaaaga
    22741 accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg cgctgctttt
    22801 tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa acgagcttct
    22861 tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg aactagcagg
    22921 taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat ttcaaccatt
    22981 attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca tcagcttcgt
    23041 cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa gacataacgc
    23101 ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca tcagagttaa
    23161 atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca gtttctgcta
    23221 ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt gttgttttta
    23281 tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc ctttcatctg
    23341 taaagattaa catttttttt cgttagatac aacagttttc attcaatttc agataaaagt
    23401 attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac aaacgtgttt
    23461 atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc ctttgaccct
    23521 ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta taataagagt
    23581 caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat ctcttaaaaa
    23641 actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat gtacccattt
    23701 ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga
    23761 ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt cagagctgtt
    23821 atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa aatgatccat
    23881 ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg caacagcaag
    23941 gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc aatggtggtc
    24001 agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat atcacagaac
    24061 aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc aaacgagtca
    24121 ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa ccaggtgttg
    24181 gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa
    24241 aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa ggtactggta
    24301 tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt caagcagaaa
    24361 atgtgatcct attcatagat gaagttcacg aaatcgtcgg tgctggttct gctggtgatg
    24421 gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa ttgcaaatgg
    24481 ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc
    24541 gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag
    24601 gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag gccatcgaag
    24661 cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat aaagccatcg
    24721 atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa
    24781 caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct cgagaagaag
    24841 atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa atgaaagaac
    24901 gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa aaaatcgttg
    24961 aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca caactgaaaa
    25021 acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta gatcgagtag
    25081 cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca atcggctcct
    25141 tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg
    25201 aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat atggaaaaac
    25261 atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa gaagctggtc
    25321 aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat gaagtagaaa
    25381 aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt cgattgacag
    25441 atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca agtaatgccg
    25501 gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt
    25561 ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt tttgacggga
    25621 tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag
    25681 acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct acgaatgtca
    25741 aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct cttcgacgca
    25801 cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac ccttctatcc
    25861 atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct aaaccagaac
    25921 gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat agcgtgagac
    25981 ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa tactgttcat
    26041 tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata aaagaggtga
    26101 gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta aaccagcttg
    26161 aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact attgtctaca
    26221 ctgaagcccc tgaacataat gaaattctgc tgatcaatga caaacgtaaa atccagccga
    26281 aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc gtttaccacg
    26341 aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt ccaaaacatg
    26401 aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa agagcgtggg
    26461 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc gaaagcagaa
    26521 gcaactcctt cggaaataag ctgaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    26581 ccagcttatt tcttggagtt aaacgcttct gtctcagcct ctttttcttt ctagcttttt
    26641 gatttttctc ttttcttttg tacttttctc ttttcatttt cagttctacg cgtgtcttta
    26701 tttttaactt tttcctcttt atggatgatt gatcgtactt tttgtgcaat ttgctcgttt
    26761 ttgtcccagt cttttacatc taataatcga cataatcgta acatctcttc ttttttctgt
    26821 ttttttcttt cacgtctttg ccattctgtt tccatgtgtc acactctcct ttctttaatc
    26881 atatgtacgc aaaataattt ttatattttt ttgattagac atttctatga ttttaatata
    26941 gtaaaaagag ccgtcacgga aaatccgtca cggctctaaa caaaatatga gaggctgttt
    27001 ttgtttggct attcttatta cttgatgcag ggcagcttgt gtcaagactt ctttacatca
    27061 aactttttaa ttttacattg ggatatttat ccgcaaacca gcgttctgca aattggttct
    27121 cgaataagaa caacggttgg tcgaaacggt cacgagccaa gatattgcga cttgaactca
    27181 tcttctcatc caaatcttct ggatcgatcc agcgagcgat tttgtgtccc attggagaca
    27241 tgattacttc ggcgttatac tcatttaaca tacgatattg gaatacttcg aattgtagct
    27301 gaccaacagc tccaatgata tattcttccg ttagatatgt tttataaagc tgaatcgctc
    27361 cttcttgaac taattgatag attcctttgt ggaaggattt ttgtttcatg acattttttg
    27421 cagtcacttt cataaacagc tctggagtaa atgaagggag ttcttcataa gcaactttta
    27481 gttttccttc gaacaatgta tccccaatct gataattgcc tgtatcatat acaccgatga
    27541 tatcgcctgc tactgctttt tcgacgtttt ctctcgaatc tgccataaat tgtgtgacat
    27601 tgcttaattt cagtttttta tttgtacgtt ccaaccagac atccattcct cgttcgaatg
    27661 ttccagaaca gattcggaca aaagcgattc ggtcacgatg ggctggattc atatttgctt
    27721 ggatcttgaa tacgaaacct gaaaattctt cctcataagg acttaccatc tgatcatctt
    27781 gtgttttgtg tccataagga gctggtgcca attcaacgaa agtctctaaa aaagtctgta
    27841 caccgaagtt agtcaatgca gaaccaaaaa atactggtgt ctgatcgcca cgagcaattt
    27901 tttcttgatt aaatgcatct ccagcctctt tgattagttc tacttcttct aagacttgtt
    27961 catataagga attttcagtt agaggatgat tttcgggaag cagtccatct tcatccaatt
    28021 ctactaatcg ttcatcctga taattttccg gacgataaaa ctctacacga ttatggtaga
    28081 tatcataaag accttctagc cctttaccca taccgattgg ccaattcatc ggataagatt
    28141 cgatatctaa tagctcttct aattcttcta acaattccaa tggttcacga ccatcacgat
    28201 ctagtttgtt gataaaagta aatatcggga tgccacgttt ttttacaact tggaataatt
    28261 tctttgtctg tgcttcgatc ccttttgcac tatcgattac catgaccgca ctatccacgg
    28321 ccatcaaagt acgataagta tcttcagaaa aatcttcatg tcctggtgta tccaagatat
    28381 ttactcgttt cccttgataa tcaaactgca taacagaact agtaacagaa atcccacgtt
    28441 gtttctcgat ttccatccag tctgatttgg caaaattacc tgtttttttg ccttttacgg
    28501 tcccggcttg acggatagcc ccaccaaaca atagtaattg ttctgtaatc gttgttttcc
    28561 cagcatccgg atgggaaatg atcgcaaaag ttctgcgatt gtccacttgt tgctttaatt
    28621 ttggattatt cataacctaa ctccatttct tccattcaat tatttatatt ttctctaggc
    28681 acagattgtc cgatctctta aagaaagaca atttcatata gggctgtgac aaaagccgtg
    28741 tagcagtctc tttttccgag taaacggcgt tctagagaca gcctatcagc aataagccca
    28801 cccaagccaa aaatacgaaa agctgttttt gcttggctgc tcttattact taatgcagga
    28861 tagctttttc acaacctttg tcaactacta caactgaatc agtatagcat ggattcagcg
    28921 aatgaaaaag ccaccaaacc ataagagtca aatatttttt tgtcagctga ctaaaagaaa
    28981 agacgtgttg cttgtttatt caagcaacac gtctttcata accaagtttc caattatctt
    29041 cttggctcgt catcttcgaa ttcttttctg aagtatcttc gttcttcttc aggttccgct
    29101 tctgtttctt ccggatcatg aaaaatgaca cggattttaa ggattcgaga accttccatt
    29161 tcttcagaaa ccagcgtaat attatcgaca tcaaacgata acttttcgcc ttcatccgga
    29221 atcatgccta atgctgtaat caaataacca gccatcgtat ctacatcact catatgcaga
    29281 tcactgtcaa aagcttcatt gaactcatca atcagcatac gtccttggat gatatattcg
    29341 tgttcatcga ttttttcata aagattctca acttcatctg attcatcatc gatttctccg
    29401 acgatttctt ctaaaaggtc ttctagtgtt gctaacccaa cgacgccgcc atattcatct
    29461 aataaaatag ccatctgatt ctgcgtcttt ttcatttcat ataacagatc atcaataaag
    29521 accgtttcag gaacgaataa aggctcctgc atgattttct tgatatctaa attatcaaag
    29581 ccgaatttat gggcagcttt tagtaaattc tttgtatgaa ggattccgac tactttatct
    29641 ttgtcttcgt tataaacagg gattcttgaa tagttttcgg ataaaacttc attcacattt
    29701 tcttccacag tgtcgttgat atcaatcata aatgcatccg ttcgtgggac catgacttct
    29761 cgggcaactt tcgtatcaag agaaaaaacc ccttggagca tttctaattc ttcattattc
    29821 aatacgcctt cgttttcaag catataacgc atttcatctc tggtcatttt tgaatcttca
    29881 tcatcaaaag tcatcggcgt gatacgcgcc aataaacttg tggaagcaga aagcagccag
    29941 acgaagggct tagctactac accaatcaag cgtatcagcc ctgaagtgaa ctttgcgact
    30001 tcttctgatt tatttaaagc aatacgttta gggtacaatt ctccaaaaac aatcgataca
    30061 taggtcaaaa tagccagcac aatgatattt gcaatatttt ttgccgctgt gccgccacct
    30121 aataccggag ctaaccgttc cgataaagta ttagctagtg aagcaccaga caaaatgttg
    30181 accaaagtga ttcctacttg gattgtcgat aggaaattat ttggattctg caatattttt
    30241 aaaagctttt gagatttcac atctccttca tcagcttttt gctcgattcg attcttattg
    30301 atcgaaacaa cagcaatttc ggaagctgct aaaaaagcat tgatcaatgt caaaacgatc
    30361 aataataaaa tctgcgctaa cagcgactga ctctcagggt cagcattcat aatcgcattc
    30421 tctccttata aaattaattt ttagtttacg atagaaaaaa acgtggcact atagccacgt
    30481 taaaatggta tcacaaagca attttctttg caataaaaac aagtgtacta aaactaccta
    30541 cgaggtcgta gcaattttct tcttgttttc attttgcttg tcttctttga cgattttgat
    30601 tatataggaa atgatcgtct tgcagattgc gtaaaaagga acacctaaga agataccaag
    30661 caatccggca atatttccag ctactagaag aacgataatg atcgttaatg gatggatttt
    30721 cagtgatttc ccaattacat taggataaat gatattccca tccaactgtt ggacgattag
    30781 cacaaccaca caacaaagta atgcttttac cggatcattg aagactgtta ctagaaatgc
    30841 aggtgctagt cctagataag gtcctaaata tggaattagg tttgtcaatc ccgcaatgac
    30901 cccaaataag aaagcataat ccacaccaat taccatatat cctaaaaatg tgaaggtacc
    30961 aacgaataaa cattcgattg cttgtccgct aatgtaatta gccaatgttt tattcagttg
    31021 ccccaataac tccacaattt ggtttcttct tttttctggg aaaaactgtt ggatgtttgg
    31081 cactagtttt tctccgtctt tcaacatata gaacaagatg aatggtgctg taatgattac
    31141 tacggttgcc gaagcgatcg ttccaacgat cgaacctaaa ctattggata ctccactaag
    31201 gaattgctga atgattgtcc cgtaagaaat gtctaattgc tcaaaatatt tattgatatc
    31261 tacttccttg aacaaaggca attcggctac ctcttttaac cagctttcta cctgtttgat
    31321 gaaatcaggc atattagatg ccaatgagga aatttgctgt actagacttg ggatgacact
    31381 aagtaaaatc caaataattg ctgcaactaa aaggagcagc acaattgcga cagcccatat
    31441 tcttttgatt tttgctttca tcaacagctt gacaatagga ttcaataaat agtaaagaaa
    31501 gcctgcaatc agtacaggtg caaataacgt ggaaaaaaat gtcccgatcg gttcgaaaat
    31561 aaagtttatt tctgatgaaa tcatgatcaa tgcagctatg atcaataatt caagcgacca
    31621 aaacattagt ttagactgtc taagtttttc aaacaataga tttcctcctt tctttcttac
    31681 tagtatatat tatattcgtt aaaaattctt ttattctttt tttatttttg aaaacttgcc
    31741 atacttttta tttgttttat tttccagtat actatacgaa aggaaggtgg ctagaccatg
    31801 aaagttgtac aaacaaaaga tacaatgagc aatatttact tagatgctgt acggatcagg
    31861 catcaagttt ttgtagtcga acagggtgtg cctttgtctc gagaaataga caaagatgaa
    31921 gcccattgta tccacttcgt cctgtactca gataaaaaag aaccgcaagg aactgtacgt
    31981 cttttgcctt tagaaaacgg aaaaatgaaa ttacagcgga tggcgattct ttctgaatac
    32041 agacaccaag ggttaggaaa aatattgatt gaagaagcag aaaactttgc taagaatcaa
    32101 ggctataata cgattttatt aggggcacag tccacagctg aaacttttta cgaaaaacta
    32161 ggatatacag cttacggcga tccttttgaa gatgctggca tgcctcatat ttatatgaaa
    32221 aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca gcaagctttt gagaaaaatc
    32281 tctcactggc ttactgttct tttttgtgtt tacttacgcg acaattgctt gtaacggtta
    32341 taccaaatat caatatagcc ttccgagaac gggccatctc cattattgat ccagtcaacc
    32401 agaatcttca cattttcttt taggatgaag tcaatatcgt tcgggtaatt catcattttg
    32461 ctgtgcatct catattcatc gacatccaat agtcgtttct cgccgtcagg aaataccttg
    32521 atatccaagt catagtcgat atatttcaag gcctcatcgt ccagcaaaaa aggtgaagct
    32581 agattacaat aataggaaac ccccttttct ctgatcattg ctattacatt gaaccagtat
    32641 tttttatgaa aataaacaat cgctggttcg cgcgttaccc atcggcgtcc atcagattca
    32701 gtgactaatg tgtgatcatt cacgccgatc atagagtgtt cgcttgtttt taataccatg
    32761 gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt aactttggat cgtcacaaac
    32821 tctccttctt ttggaactcc catagcaacc cttccttaag cagcattttg tgctttgtgt
    32881 ttgggtgttc attacaccga catcataatt attatatcaa taaaaatgag ctttgcctag
    32941 aaaggattcc tattgtttcg atttttctct aactgatcta ccagtttcat ttggacctta
    33001 ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact tcgaagtttt gttctcggta
    33061 aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa ggagaacatg ccatttcaga
    33121 tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct gccaaacaaa gagttcctgc
    33181 ttttcaaatg ggagattttg ctcttcttct gctactaggt catcaaacag atcgatttct
    33241 ggctttgttt cccattcttt tttcaatcgc tcatactcct cttgtgtcac ttccatcatt
    33301 ggaaatgtcc acatattcgc cagcaatttt tggctatctc tttcttcaaa ataatacgct
    33361 ccagaatgat tttgaagagc tgcactgata taataaacgt cttttggctt tgcttttttt
    33421 gtttttacag gaaaactcgt ttggattcct cgtttgttag ctaaacaaaa ggcttggatc
    33481 ggacaagtct cacactttgg agaagttggt gtacagatag ctgaacctag atccatcatt
    33541 gcttggttga attcacctgg atgcttttca tcaatgattt tccgcatcgc ttcatcaaag
    33601 attttcctgc tagaagcttt tgcgatgtct gcttcaatgc aaaacaatct gctgactact
    33661 cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa atgcgatact tgcaatcgcg
    33721 cctgtcgtat atggtcctat tcccttcaat gagctaattt cttcaggcgt ttgcggcatt
    33781 tccccatcaa attctgacat gatctgttta gcagcagctt ggatgttccg ggctcttgag
    33841 taataaccaa ggccctccca tgcttttaaa agcttttctt ctggagcatt tgctagctct
    33901 tcaattgtag gaaaccattc catgaaacga taaaaataat cgatcactgt gtctactctg
    33961 gtttgctgaa gcatgatctc agagatccag atacgataag gatcacgatt atagcgccaa
    34021 ggcaggtttc ttttttcttg ttcataccat tgaatgaact gatcttgaaa ttcttttgtt
    34081 tctttatctg tccatttttc ccattcttta ttcactttcc tcttcctttt ccgtaatgaa
    34141 tcgctggatc atatccaaat caaatccttt ttgatataag ctttgcttta cttttagatt
    34201 tcttttttta ggttcaaacc ttaaatttct tcgccatagt ttatctcctt gtttgactaa
    34261 ataatcatat tctatttctt gatctgcttc ttgcggaaga tcagctatcg cttgttggat
    34321 cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg atttttctca ataattcttt
    34381 ttggcttttt ccgtgatttt ttccaaacgc tttttcggct gtctgatgtg cattttgaac
    34441 ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg atttcaggtt tcaaaccttt
    34501 ttgctgcatc tgctgcgcta atttctttgg tcctttgtca cttaagcgca tattcgtgcg
    34561 cacataactt tccccataga ccaaatcatc gacaaggtcc aattctttta agcgggctac
    34621 gattttattt cgatcttctt gaaggatttc cttttctttt aagtatgttc ggatttcttt
    34681 ttccgatcgc aactgataac ttaagtaatt cattgctagg tgaaaaccga aatcagctcc
    34741 gctactcttc ttgatctctt ggatagtttc ttcgtctaat tcccgtcctt ttaataatcg
    34801 atagcgtact aatacatctt cagatacttg taaagcttct ccttcagata aatctacgcg
    34861 atagaaaggg cctttgcctt tactgattct cgtaacggtt aacataaaca tcctcctcga
    34921 acatatattc tctatcatac cacagaaaat ccccgtaatc aggtattata tgttagaata
    34981 aaccacagaa aaaagaaaaa gggagatact tatggcagaa acaattgtca aaactggaca
    35041 aacggttcct ttgaaaatca agcgtttagg cataaatggt gaaggaatcg gttatttcaa
    35101 acggttgatc atatttgttc catatgcact accaaaagaa gaagtactag tgaaaatcac
    35161 gaaagcaact cctcgctatg cagaaggaga attaataaaa gtcaaaaaaa caagcaaaga
    35221 tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt ggctgtcaat tacaacactt
    35281 agcttatcaa gctcaattgg attttaaaaa ggatctgctt ttgcaagcat tggaaaaatt
    35341 caaaccagca ggctttcgat cttatcaact tttgccgacg atcgggatgg atgagccttg
    35401 gcattataga aacaaagcac aatttcagtt acgaaaaaat aagcagactc aaaaaattga
    35461 agctggttta tacgaagcaa actctcatca gttagtccct ataacagact gcttggttca
    35521 agaacctcga acacaaaaag taatgaatac tgttgttcaa ctattgaata aatttgatgc
    35581 acccatctat gatgaacgga caaacagtgg aatcttccgc acaatcatgg ttagaatcgg
    35641 tgttaaaact ggggagttac aagttgtttt tattactcgc agtaaaaaat tcccgcaaaa
    35701 aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa gttgtttcaa tcatgcaaaa
    35761 tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa acgatccact tatggggaaa
    35821 agaagccatt gaagaacaaa tcaatgaagt gactttcgat ctttctcctc gtgccttctt
    35881 tcaactcaat cctcagcaga ccgaattgct ttatggcgaa ggaatcaaag cattagatgt
    35941 ccagcctaat gaaacgatcg tggatgctta ttgcggagtt gggacgatcg gcttaagtgt
    36001 agcaaaacaa gccaaagaag tacggggaat ggataccgtt cctcaagcaa tcgctgatgc
    36061 aaaaaaaaat gcggaaagat taggatatca caacactcga tatgaagtcg ggacagcaga
    36121 gcaagttctg cctaaatggt tgaatgaagg ttttcagcct gatggtatca tcgtagatcc
    36181 accacgtacg ggtttggata acgcattgat ccaatcatta ttgaaatacc ctccaaaaaa
    36241 acttgtttat atctcctgta atgtttctac acttgccaga gatctggtta atttaagtaa
    36301 aaagttcaag attgaatatt tacagtccgt cgacatgttt cctcagactg cacgttgtga
    36361 agttgtcgtg aagatgacga gactcgctga ctaaatgaaa gaggttgtga aaaagtctgc
    36421 cctgtatcaa gtaataagaa cagccaaaca aaaatagctc ttcatgtttt atgaatactg
    36481 tacttgtgac cgaagagtta acaggaggac actatttatt taaaaaaagg gttgtgacat
    36541 agtttttgtc acagcccttt ttgattatta ttggatattc acgtctatca cttgataaaa
    36601 tgcatttgtc gtatcgctga tattccatac tgctaaaatc acattatatc cttttcgatc
    36661 ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga acagttgctt tgtcgtctat
    36721 ttttgtgatc aattcaaaat cactaaattt caaaggttga ttaggattcc aagttggttt
    36781 ggtaatgtag taatcccaag tacttgtccg atgttgtgct gttaaattcc aagtaatatt
    36841 taatgttcct ggattaacca tgtttttatg ccatctgtta gatgtctgtt catctaaagg
    36901 ctcaaagccg ctgactcctg cactagcaat ttttccatca atgaatgtat ttttggtagc
    36961 ttcgatactt tgcggttccc attgtgctct tccgacattt tgatttaagt ttcctgctgc
    37021 tgatgttcct aggtatgcac ggcttcctgg agaagtaata taaccatgcg caaaacctgg
    37081 aatgctaaaa ccgaacaacc ctaaactaac taacccaata aacaataacg tactaatctt
    37141 tttcatgtag aaacctcctt catttgataa ccacattgta aacgctttat tgtattaaca
    37201 caaacattat gcttttataa ttttatgttg aagtaaaaaa ggtgaaaaaa gtaagatgca
    37261 gattataaca gaagtagacg agaaaagtac aaatagaaaa catgagaatc aaatcatatg
    37321 atctttttca aaaacatcat tcatataaac aaatgttcaa ctttttaata acaacaatta
    37381 tcaatttttt tcttacttca caaactaaat acaggtcttg ctgtgagcct tcatggtaaa
    37441 ctaatattat agaaaggagt tagtaaaatg aagatgataa aagatattcg tttttgggtt
    37501 tgtgttatag gaattgtcat attaggcttt ttatcagggt tgcttagcgg aaatcccgga
    37561 aaatattact attctttgca acttccacca tttgcaccac ctagctggat atttgggccg
    37621 atgt
//
LOCUS       DASJSZ010000019        34961 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.019, whole genome shotgun sequence.
ACCESSION   DASJSZ010000019 DASJSZ010000000
VERSION     DASJSZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.019"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            157..1863
                     /gene="efbA"
                     /locus_tag="U4B03_000910"
     CDS             157..1863
                     /gene="efbA"
                     /locus_tag="U4B03_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEN2067782.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2021..2923)
                     /locus_tag="U4B03_000911"
     CDS             complement(2021..2923)
                     /locus_tag="U4B03_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2067783.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2920..3612)
                     /locus_tag="U4B03_000912"
     CDS             complement(2920..3612)
                     /locus_tag="U4B03_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEN2067784.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3655..4296)
                     /locus_tag="U4B03_000913"
     CDS             complement(3655..4296)
                     /locus_tag="U4B03_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2067785.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4334..4648)
                     /locus_tag="U4B03_000914"
     CDS             complement(4334..4648)
                     /locus_tag="U4B03_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067786.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4636..5988)
                     /locus_tag="U4B03_000915"
                     /pseudo
     CDS             complement(4636..5988)
                     /locus_tag="U4B03_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5985..6917)
                     /locus_tag="U4B03_000916"
     CDS             complement(5985..6917)
                     /locus_tag="U4B03_000916"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEN2067787.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6914..7690)
                     /locus_tag="U4B03_000917"
     CDS             complement(6914..7690)
                     /locus_tag="U4B03_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEN2067788.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7744..10929)
                     /gene="carB"
                     /locus_tag="U4B03_000918"
     CDS             complement(7744..10929)
                     /gene="carB"
                     /locus_tag="U4B03_000918"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEN2067789.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10934..12010)
                     /gene="carA"
                     /locus_tag="U4B03_000919"
     CDS             complement(10934..12010)
                     /gene="carA"
                     /locus_tag="U4B03_000919"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEN2067790.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12015..13295)
                     /locus_tag="U4B03_000920"
     CDS             complement(12015..13295)
                     /locus_tag="U4B03_000920"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEN2067791.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13314..14240)
                     /locus_tag="U4B03_000921"
     CDS             complement(13314..14240)
                     /locus_tag="U4B03_000921"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEN2067792.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14253..15536)
                     /locus_tag="U4B03_000922"
     CDS             complement(14253..15536)
                     /locus_tag="U4B03_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEN2067793.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="U4B03_000923"
     CDS             complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="U4B03_000923"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEN2067794.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16324..17235)
                     /locus_tag="U4B03_000924"
     CDS             complement(16324..17235)
                     /locus_tag="U4B03_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2067795.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17232..17696)
                     /locus_tag="U4B03_000925"
     CDS             complement(17232..17696)
                     /locus_tag="U4B03_000925"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEN2067796.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17701..18399)
                     /locus_tag="U4B03_000926"
     CDS             complement(17701..18399)
                     /locus_tag="U4B03_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2067797.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18386..18772)
                     /locus_tag="U4B03_000927"
     CDS             complement(18386..18772)
                     /locus_tag="U4B03_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067798.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18919..20589)
                     /locus_tag="U4B03_000928"
     CDS             complement(18919..20589)
                     /locus_tag="U4B03_000928"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEN2067799.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20772..21005
                     /locus_tag="U4B03_000929"
     CDS             20772..21005
                     /locus_tag="U4B03_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN2067800.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21104..21535)
                     /locus_tag="U4B03_000930"
     CDS             complement(21104..21535)
                     /locus_tag="U4B03_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEN2067801.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21614..22027
                     /locus_tag="U4B03_000931"
     CDS             21614..22027
                     /locus_tag="U4B03_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEN2067802.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22065..23213)
                     /locus_tag="U4B03_000932"
     CDS             complement(22065..23213)
                     /locus_tag="U4B03_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2067803.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23335..23931)
                     /locus_tag="U4B03_000933"
     CDS             complement(23335..23931)
                     /locus_tag="U4B03_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2067804.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="U4B03_000934"
     CDS             complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="U4B03_000934"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEN2067805.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27161..27592
                     /locus_tag="U4B03_000935"
     CDS             27161..27592
                     /locus_tag="U4B03_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2067806.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27637..28986)
                     /locus_tag="U4B03_000936"
     CDS             complement(27637..28986)
                     /locus_tag="U4B03_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2067807.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29056..30219)
                     /locus_tag="U4B03_000937"
     CDS             complement(29056..30219)
                     /locus_tag="U4B03_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN2067808.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30348..30671
                     /locus_tag="U4B03_000938"
     CDS             30348..30671
                     /locus_tag="U4B03_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067809.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30854..32203)
                     /locus_tag="U4B03_000939"
     CDS             complement(30854..32203)
                     /locus_tag="U4B03_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2067810.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32272..33225)
                     /locus_tag="U4B03_000940"
     CDS             complement(32272..33225)
                     /locus_tag="U4B03_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEN2067811.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33327..34517)
                     /locus_tag="U4B03_000941"
     CDS             complement(33327..34517)
                     /locus_tag="U4B03_000941"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEN2067812.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9951 a         7641 c         6120 g        11249 t
ORIGIN      
        1 tacctaaaaa tttggatttc aacgatagaa acactgtttt ttatgtaatt agtccaagat
       61 ttttctagac ttttgcttta ctattcggcc aaattggttt ttcttctttt tttcgttata
      121 atgaatatac ttatgaaaag aaatgaggaa tgaaccatgt cctttgatgg tgtatttacc
      181 cacgcgatgg tcaacgaatt gcgtgaaacg ttactatctg gccggatctc gaaaatccat
      241 cagccttatg aaaatgaaat cgtgctggtg attcgttcac gcggcaaaaa ccatcgatta
      301 ttattatctg cgcacccaag ttatgctcgt gtacagatta cccaaatcga ttatcagaat
      361 cctgataatc cgcctaactt cgtgatgatg cttcgaaaat atttggatgg cgctatttta
      421 gaagacatcg aacaaatcga aaatgatcga gtgatccatt tccactttgc taaacgaaac
      481 gagttgggtg atctacaaaa tatcatattg atcgttgaat taatgggacg tcatagtacg
      541 atcgtcttag ttaatcgaga aactggaaaa atactggatg cgatcaaaca cattggaagc
      601 tctcaaaaca cttatcgttc tttgcttcct ggtgtagaat atgttgctcc gccaaaacaa
      661 gaggtcttga acccatttag ctccgagaaa gaaaaaatct ttcagcggct ttcccaaact
      721 gaactagatc caaaagcaat ccaacgtcag tttcagggaa tcggtttcga tatcgctcaa
      781 gagctgacca aaagattact ggaacgtcct aatgaaaaga tggttgtatg ggacgaattt
      841 ttctcagcca tcagccatca gcctattcct actttttacg aaacggaaaa taaagatttt
      901 ttcacaccga ttgcttatca ggtattgtcc gaacaggcat ctgctgtaac agcttatcca
      961 acactcagtc aattattaga tagctattat cacgaaaaag cagagaaaga tcgggcaaaa
     1021 caacaaggcg gagaactgat tcgaaaaatc gaaaatgaac taaaacgaaa taaaaataaa
     1081 ttgaaaaaac gtgaacaaac actcaaagaa tccgaaaacg ctgagaatta ccgtagaaat
     1141 ggcgaactac ttactacttt tctgacacaa gtaccacgcg gcgcaaaaga agttgtttta
     1201 cctaattatt atgaagaaga tcggccaatc aaaatagcac tagatccggc attgacacca
     1261 aatcaaaatg cacagaaata tttccatcga taccaaaagc tgaagaacgc tgttaagctg
     1321 attggcgaac aaatccaaga agccaaagat gaaatccaat atcttgaatc tgtcttatct
     1381 caactggaaa ttgccggccc aatggatatc gaagcaatta aagaagaatt gacttcagaa
     1441 ggatacctga aaaagaagaa cttaaaaaaa caaaaacgta agaaaccatc tcagcctgat
     1501 caatactttt cttctgacgg aacgctcatt cttgttggta aaaacaatct tcaaaatgac
     1561 cagctgacaa tgaagacagc taaaaaaacc gactattggc ttcatgcaaa aaatataccc
     1621 ggttcgcacg tcatcattaa aagtgacaag ccctcagatg aaacaattac agaagctgcc
     1681 gagttagctg cctatttttc aaaataccga tactctgccc aagtgccggt tgatcttgta
     1741 caggtaaagc atatccgaaa accaaatggc gccaagcctg gttatgttat ttatgaaaac
     1801 caaaagacga ttatcgtgac accagaagaa gaaaaaatta cagctatgaa acaaaacagg
     1861 taatcaatga gtgtttctag aatcatattt gcgtactttc ccacaacagc aattagttat
     1921 aaattgtttg ctacaaaaag aggagttgtg acaaaattct gtcacaaccc ctcttttttg
     1981 atcaacggtg ttcaataaac agcgttctca tctgcttttc ttagctctgc tggtccagta
     2041 accgagtaag ctctttgata aatctttgag cgatttcact gagcggacgt ttcgccgaat
     2101 aaatgatata aaattcattt agttcgcctt ctgccaaagg gattaaccgc aagtgctcgc
     2161 gaatccctcc aatcacaata ccagatcctg aagcgtaagc atcagtttca agaacgatat
     2221 tcatcaaagt acctcgatca ctcgtataaa tcactgtttc ttgatcaggg atctcgataa
     2281 ggtcttcata aaaatacggg aaattatttc catcctgtgt aaaacgaact tgaggataag
     2341 ataacaattc ttctttggtg atctctgcgt gttcagctaa tggatgtcct tgccttaaaa
     2401 aaatatgcgt tttgaatttt ccaatgctgc ggccacttag ctctccttgt tgtaaatatc
     2461 ttctgatacc agcttgatta tgctcattca tataaataat gccgatctca ctgtgctgct
     2521 ctttgacttc ttcgatcact ctcgctgttg tcgtctccat gatccgaaat tgtttgatct
     2581 cgtctttgaa ttcctcgagt agctgtgcgg tcaaaggacc taagaaatca taatgctggg
     2641 cagcgatcga aaaattgtct ctcttttcac tcgttagata gtgagtttct aatgtatcaa
     2701 gctggaccaa tattttctcg gcacgattta agaagtcttg cccttcttct gttaaatatg
     2761 cccctcgatt cgttcgagta aacagcgtga tccctaactc ggtttctaat tctttgatac
     2821 cagctgaaag acttggctgg gagacaaaca ttttctttgc cgcttctctg aaactaccat
     2881 tgtttgctac agcgaccacg tatttcatct gctgaatatt cattgctcaa actcctcgaa
     2941 ataataaatt tctcttccat tcaagggctg gattggtcgg ttgttcggtc gagcatactg
     3001 accatcgaag gggagaagaa actcttggtt cagttttcct acatgatcca ttacaaacca
     3061 ttggattggc ccgctggtag gcggagttgt caatgagcca atataatgga aatggctttt
     3121 ttgctccgga aagaacacat ccggatttac ccaatgctca tgtttttcta agttccaact
     3181 ttccccattt tcttgattat acagccaacc ttcctgtacc attagctggt acatgacacc
     3241 cacaactaga ttctcttttt tgctgttcat atgaacaaaa tgaaattcaa tatcttcctg
     3301 ttttccgttg atgatatgtt cagaaggcaa gtggaaatga atatctgtca aataatagcg
     3361 ttctttcttg aactctacat aactgagttg gtcaaaaggt actaaatgaa tcgtgttttt
     3421 gaattccttg tcagtaaacc gttgcttttg ataatgaaag gaaagcgtgt ctccttctat
     3481 tttggtttct tcccccttgg tcaaatgaat aggcgactgc aatggaaatt ccgcacctct
     3541 cgcaaaccaa tcacataaac tcgcccaatt ttccggacct ttgtctccag aatagcccca
     3601 ttcgacatcc atattctttt tcaactatac ttccccctgc atctcatatc atttttattt
     3661 ttcttctttc tttacaatcg ttgagacggt ttctaaaaac gcctcttttc cataaaattc
     3721 ctggttcaat agttccggaa atatctcttt aaagaaatac tgaacagaag gccgatgttc
     3781 aaagctttta atcgtagtca ctgcctcaat gaacatctcc ttataaactt tttccgggtt
     3841 atttttgatg atttcatcaa aagattcata aagaaggtca accttatctg aaatggctaa
     3901 tagttctcct tctacggttt catcttttcc ttcgaacaac cgacggcgat aaacttcttg
     3961 gaactcagct ggaatctctt ggttgatgaa ttcgtccgtc atcttttctt caactgtttg
     4021 cagcatccct cttaactctt tgcttgcgta tttcaccggc gttttgatat caccaataaa
     4081 acgctctgtg tagtcatgat tcaatgattt ttcatagagc agtttccaat cgatcggttg
     4141 tccattcgct tcttcaatgt ctcctagaac ttgcgcaata gatgctacac gatacgaatg
     4201 tgctgcaact gtatgctctg tatatttaaa aaatccaggt gcccttgtga tcgtctctaa
     4261 attgttcaat cctaaaataa attcattcaa ccccataaaa gttacctccc tttgagattt
     4321 ctttttccga atatcatatc attaaataac tgatattcgc aacatttccg ctctttacca
     4381 tttttgaaac agctccctta ccaaaaaata gagaaaatct atatataata gaaaaaagag
     4441 aagagattat tctcttcttt ctgctaacca gtcttagaaa aatgaatttt aaaaaaatca
     4501 aaaactgatc acgatagaag tttcttttca tacctgatga aaagatgctt ctcgcaatca
     4561 gtttttggat ttttgctatt ccaattgatg attctggaga cacaaatcgg atttctctat
     4621 cttcatcaag cgcgcttaaa tctgccaatt ttcaggatct tttttccatt cttttagcag
     4681 agttaattct tgctcatcga tataacgatc ttctaaagct gcttcaatca atacagaata
     4741 attggtcaac gtcataagag ggatttcggc tttttcgaaa tttgcttttc cttttggcaa
     4801 ctcatacgta aagatagccg caacaccaag gatatccgca ccttctcttt ttgctgcttc
     4861 agctgcttcc aagacacttc cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg
     4921 accttcaaat atacgtcctt cgatttggtt acctttccca tgatctttcg ccttacttcg
     4981 aatatagacc attggcaaat ccagtatttc cgctacccat gcggcatgag ggattcctgc
     5041 tgttgcagtt ccagcaatca cttgtacttc agggtaagct tctttgattt tgcttgctaa
     5101 tccttttgcg atctcttttc ggacttttgg atagctcatc gtgattcggt tatcacagta
     5161 aattgggctt ttgattccac ttgcccatgt aaaaggatta cttggattta aaaatactgc
     5221 ttcaatttct aataagtctt ttgcgatact ttgttcaaca ttcattttag gttagctcca
     5281 ttctgtttga atcgtttgat atgctttata aggattagtt gactgagtga ttggtcgacc
     5341 tataacgata aaagttgagc cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg
     5401 atctcctgcg actgttcctt ctggtcttat acccggagtt aagcaaacaa attcgtctcc
     5461 tgccatttct ttgatcgctt ctacttccca agcagaagaa accacaccat ctaatcctgc
     5521 ttttttggca cagctagcat aatggatcac acttttttct aaagaaactt cgattagttg
     5581 atcggcatgc atttcttctt cacttgtaga agtaagctga gtgatagcta gaagtagcgg
     5641 acgttttcca ccttctggag tcccctcttc taatcctcgc attgccgcct ccatcatacg
     5701 aataccgcca gcggcatgaa cacaggtgag atcaacacca agctttgcca atccccgcat
     5761 tgctttttcg accgtattcg gaatatcgtg aagtttcaga tctaaaaata cattatgacc
     5821 agcctctttt agataacgta cgatttcagg tccttcttgg tagaacaatt ccattccgat
     5881 tttcacaaac aatttttctt ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct
     5941 agttgaaaaa tctaatgcaa taattggtcg ttggtctagt tgattcatcg ttcacgttcc
     6001 tctctcactt ctttgattaa tctttccagc gattcgatgc ctagttcgct cattcgcaca
     6061 gggagctctt taattagttt aggacagata tacggatcgg tgaaattcgc ggtccctata
     6121 gctacagcac ttgctccagc cataaacatc tcaagaacat catccactgt ctgcacgccg
     6181 cccatcccaa tgattggaag gctggatata cttgctactt ggtgaatcaa acgaatagcg
     6241 acaggcttga ttgctggtcc ggagagtcca cctgtttgat tggctaaaat cggttttctt
     6301 gtcttaaggt caatccgcat accaagaaga gtattgatca tcgtaaaccc atctgcccct
     6361 ccagcttcga ttgcctgagc gattggaacg atgtctgtta catttgggga caatttgaca
     6421 tagacaggaa cagaagaaac tttctttact gcttgtgtta attgaaaagc aacatcagaa
     6481 tctgttccaa aagcaattcc gccatgtttg acatttgggc aagaaatgtt cagctcgatt
     6541 gctttcacat ttggtgcttc gccaattttg ctacaaactt ctacatagtc ttcttcacaa
     6601 gcaccagcaa catttgcgat gatgggtaac tcattgtact cctctaattc tggaagaagt
     6661 ttatgcataa caacgtctag accaggattt tgcaaaccaa ttgcattcaa catgccagat
     6721 ggtgtttccg ccactcttgg cgtgggattg ccaaagcggg catttggagt cgttgccttg
     6781 accatgatcg atccaagctg attcaaatca tagtattttc cgtattcttt gccaaaaccg
     6841 aaacagccgc ttgccggcat gataggattt tttaattcta ggccaggaag tttgaccgct
     6901 aaaggattct cattcatata attacctctc ctattttaaa aatcggtcct tcgtcacaaa
     6961 ctttcacact ttttgttcct gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag
     7021 cccctatccc acatgccatg cgggattcta atgataactg gacattttga tgcgaagaaa
     7081 acagctgttc cactgtcttc aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt
     7141 ccgtcttcgt tcctaacaat aaattaccga catttccgtg cataccgtat gttccatcgt
     7201 cagtagcgat tcttgtttct cctagacgat taaattcttt ttcataataa acgacttcat
     7261 gtgtggcaaa acctaaaaaa tgcgttggct taattccttt ttcgactaac tgttttgata
     7321 actcatatag aggagggaca ccgattcctc caccgattat aaatacttcg tctcctgtat
     7381 gaagattgga caaatcaaaa ccattgccta aaggacccat gacatctaaa tggtctccgg
     7441 cagaaagagt agagaagact ctagtcccct caccttccac ccgataaatg atacggcaag
     7501 tatttgtttc gtggtcgata tgattcaagc taatcggtcg gcgtaatagc aagtctgcac
     7561 gcggcacgag aatatgcaag aactgtcctg gttgctgcat ttcttttact agatcacctt
     7621 tcaatgtcat ttcaaaaatc tttggtgcca gctgtttttg ctgaacgatt tccatcattt
     7681 cttgtttcat ctgtcccctc ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg
     7741 ccgttaaata gggttagttg aaaaagcacg ggattccatc actcgaacga tcgcatctgc
     7801 tgtgtctaaa gaagtaaaca atggaatacc atgttcaact gcttctcttc ggatgatgaa
     7861 accgtcttta gaagcatttc cccgatcttt atcaatcgta ttgacaacca cttgtgtttt
     7921 accatctctt attaagtcga ctacattttt ttctgctgct tcctcagaaa ttttcgctac
     7981 tgtttcaacg accagaccat ttttttcgaa ataagcagct gtatgtttcg ttcctatcag
     8041 actatagcca atttctgtaa aacgtttagc taattctagt gcttcttctt ttgtttcgtc
     8101 cgcaatcgta aataatacgg ctccatattc aggcaagtgt aatccagaag cttcgaatgc
     8161 cttgtacaaa gctttgtcta attgtctgtc agatcccatt acttctccag tcgatttcat
     8221 ttcaggccct aagtaagtat caactttctg caatttcgta aaagaaaaga caggtgcttt
     8281 aacatgaact tcttttgttt ctggataaag tccgttttca taaccaagtg tctttagttt
     8341 ctctcccaaa atagctttcg tcgcaatttg tgccattgga attcctgtca ccttacttaa
     8401 gaatggaaca gtccgacttg ctcgaggatt gacttcgatc acataaacct cattttcatg
     8461 gatgacaaac tggatattca tcatcccgac acaattcaaa cctaatgcta agcggatcgt
     8521 gtaatcttcg atcgttttct tgatcgattc agataatgtt tgaggtggat acacagccat
     8581 tgaatcccct gaatggacac cagcacgctc aatatgctcc atgatccctg ggatgagtac
     8641 tgtttcaccg tcacaaatcg catccacttc gcattcacca ccaagcaagt aacggtccac
     8701 tagtactgga tgttctggag atgctttcac agcatgttcc atgtaatctt ctaaatctcg
     8761 ctggttttca acgatttcca tcgcacgtcc gcctaataca tagcttggac gaacaagaac
     8821 tggataacca attctcgaag cgataaccac ggcttcttct ttacttgtag cagtatcccc
     8881 taaaggttga ggaatatcca actctttcag cgcttgctcg aataaatcac gattttccgc
     8941 acgatccaag tcttcgatcg ttgtccctaa gatttttaca ccagcagcag tcaaaggttc
     9001 ggctaagttg attgctgtct gaccaccaaa ttgaacgatt actcctatcg gctgttctag
     9061 ctcgatcaca ttcatcacat cttcgaaggt caatggctca aaatataatt tatccgaaat
     9121 agaaaagtca gtcgaaactg tttcaggatt gctgttcatg atgattgctt cgtatcctgc
     9181 ctgctggatt gctttgacag aatgcacagt cgcatagtca aattcaaccc cttgtccgat
     9241 acggatagga ccagatccca gtactagaat tgagggcttt tctgatcgtg aactttcatt
     9301 ttcatattca taggaactat aaaaataagg tgtttttgat tcgaattccg ctgcacaagt
     9361 gtcaaccatc ttatatacag gaatcaaatc attttctttt ctcatctggc gaacggcttc
     9421 ttcgtttatt tcccacagct ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt
     9481 tttcaatatc ttgtcatcca acggctgctg tttcaactgc tgttcgattt ctacgatatg
     9541 caacagttta tctaaaaaga ataaatcgat ttttgtcaaa tccgctaatt cttctatggt
     9601 gtaccctctt ctaatagctt cagctacata aaacaggcga tcatcttgtg ctcgaaccac
     9661 tttttggatt aggtcatcgt ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt
     9721 accgatttct aaagaacgca cagctttcaa taaagactct tcgatattcc gaccgatagc
     9781 catcacttca ccagttgctt tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt
     9841 atcaaacggc cagcgaggaa ttttagctac aacataatct aatgcaggtt caaattctgc
     9901 aaaagtcgtt cctgtaacag gatttttcat ttcgtctaat gttaatccaa gcgctatttt
     9961 agcagctagc ttagcgatcg gatagccagt cgccttactt gctaacgcag atgaacgaga
    10021 aacacgcgga ttgacttcaa tcacataata attaaaacta tgtggatcaa gagcgagttg
    10081 gacattacag cctccctcga tcttaagtgc tcgaataatc gaaagagaag catcacgaag
    10141 catttgatat tcatggtcag acaatgtttg actaggggca aatacgatag aatcacctgt
    10201 atggataccc accgggtcaa aattttccat attgcagaca acgattgcat tatcagccga
    10261 atctctcatc acttcatatt caatttcttt aaaacctgca atacttcgtt caatcaaaca
    10321 ttgggtagcg ggggaaagac ttaatccatt ttctgatacg atacgtaatt cttcttcatt
    10381 ttcacacata cctccaccag ttccgcctaa agtaaaggct ggacgaacga tgacaggata
    10441 gccgatttta tttgcaaaat ctacagcttc ttctactgta ttgacgattt cagactcggg
    10501 gattggctga ttcaattctt tcatcaactg tttgaataga tcacgatctt ctgcttgatc
    10561 gatcgcagaa agctttgttc ctagaagttc tatgttcaat tcttctaaaa tacctgatgc
    10621 tgccagctcc attgccatat tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc
    10681 tggacgctct tttcgaagaa tccttgatac aaattcaagt gtgatcggtt cgatatatac
    10741 ttgatctgcc acttctttat ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac
    10801 ttcatatcct tcttctttta aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc
    10861 ggcttgacca atgatgatcg gaccagatcc aatcaccata attttcttga tatctgtacg
    10921 tttcggcatc tgtttactgc tcctttcctg cgtccatcat ttccataaat tcatcaaata
    10981 aatgtagtgc atcatgcgga ccaggggcag catctggatg aaactgcaca ctgaaagcgg
    11041 gataatctct gtgtttgact ccttctactg ttccgtcatt tacttctaca tgagttacga
    11101 tgagtttttc tggatcaatc gtggcttcat cgactgcata gccatgattt tgagaagtaa
    11161 aatcaattct tccggtcgct acttctcgta ccgggtgatt taatcctcga tgtccgaatt
    11221 tcattttata ggtatctgca ccgttggcta aagcaaataa ctggtgaccg agacaaatac
    11281 caaaaatcgg tacttttcct tggatttttt tgatcatttc gattgcttct ggcacatctt
    11341 ttggatcacc aggtccattc gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct
    11401 cggcatctgt gttataggga agtactgtga ggttacagtt tcttttactc aattctcgta
    11461 aaatactatg tttcaagccg aagtcaatga caatgacttt ccgacctgtt cctggacttg
    11521 gataaggttt gttagttgag acttgctgca cttgattggt tgctagaacg gttgctttca
    11581 gctgatcaaa tgcatgttcg aaggaatctc cagcatcaac tatactagct ttcatcgttc
    11641 cgacttcacg gattttgcga gtaagggcgc gcgtatcgat acctgatatt ccaggaattc
    11701 ctttttgttt caaaaatgaa tctagcgtca tttggtttcg ccagtttgaa gctacacgtg
    11761 catgttcttt tactactact cctttgcatg ttggagaaat cgattcataa tcatctctat
    11821 ttaccccata attacccacc ataggatagg taaatgtaat gatttgaccg ttaaaacttt
    11881 gatcagtgat agtttcttga taaccagtca tacttgttgt aaaaacgatt tctccgaaga
    11941 cactagcatc tgcaccaaaa gcctttcctt cgaaaactgt tccatcttct aatataagca
    12001 aacgttccat acttttattc tcccttctgc cacacaagtt ttccgttgac aaaagtcatt
    12061 tgtgtttcgc cttttacttt ccagccgata aaaggtgtat tctccccttt tgataaaaag
    12121 tcttctttat cgatcgtgca agtttgtgta atatcaaaaa ctgctacatc agcgggggca
    12181 ccaaccgtta gcgttcctgc attcaaacca aaaatctcag ccggtttaac tgccaaccaa
    12241 ttgatcactt gttccaacgt gaatcgacca gtttctacaa aatgagtata gatcagttga
    12301 aacgctgttt cgcttcctac gataccaaat ggtgatttca tgaaactttg gcttttttct
    12361 tctaatccat ggggggcatg gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct
    12421 tcgatcaaag cttctctgtc ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg
    12481 aaatcttcgg gaatatcttc gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt
    12541 acgtggattc ctgctttttt tgcgtctctg attacacgga cactttcttt agtagaaaca
    12601 tggcaaacat ggtaatgaac accagttgct tcagctagca acagatctct agcaatttga
    12661 gaagactcgg ttacgcttaa gatcccggga agaccaagtt cttctgcctt cttgccagcg
    12721 tgcataacgc cgccaaataa taaagattca tcttctgtat gggctactag ggctttattg
    12781 ttttttgcag cttctttcat agccagatac atcgtcccag ctgtttggac acctacaccg
    12841 tcatttgtga aagcaaaagc accttcttca atcagtgctt cttgatcaac taatttctca
    12901 cttcgcagat tttctgtgat tggcgcatac tgcaatacct tcacaactgc atcttttcta
    12961 atgatgtcat atacttgtct cagcttttct gccgtatcag gtacaggatt taaattcggc
    13021 atcgcgcata cagtagtgaa accgccacga gcagctgagc gagtcccagc ttcgatcgtc
    13081 tccttgtatg taaatcctgg ctctctcaaa tgaacatgga cgtccactaa accaggtgtg
    13141 atcaattgtc cctttgcatc aaacacttca tcaaattcgg cttctgagaa tcctgtaccg
    13201 attgctttga ttcgcccatc ttctatccaa atttctgctg gtgtcgtcat attttttcgt
    13261 gtattgatct gaccgttttt aatgagtgtt ttcatttttt catcctccta taattacgct
    13321 tttccattta gcactgcttc taagattgcc attctgacga atactccatt gctcatttgc
    13381 tgaacgatcc gcgactgtaa tccttccact aatgaatcag ctaattctac atcacggtta
    13441 actggtgctg ggtgcataat gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt
    13501 aacccatgtt cctgatggta gccttctttt gagaaacttt cttttccatc atggcgttcg
    13561 tgttgcaccc gaagcatcat catgacatca acttgatcta gtagttcatc caatggcata
    13621 taatgtccgt acacttcgaa ttcatcgtcg taccattctc taggacctga gaagaatact
    13681 tgtgcgccta gacgttttaa catctgcata ttggatttag ctacgcgaga gtgagtgata
    13741 tcacctacga tggcaacttt cagaccttca aaatgaccaa attcttcata aatggtcatc
    13801 aaatcaagca agcattgggt tggatgctgt ccgcttccgt ctcctccatt gataatcgaa
    13861 cactggattg ttttactttg tatcagttcg tcataatagt tctcatcacc atgacggata
    13921 accgctacgt ctacgcctaa tgctgacatc gtcagtactg tatcgtatag tgtttcccct
    13981 ttttggacag agcttgtgct tgcttcaaat tcaatcactt ctagccctag ttttttctcc
    14041 gctacatcga agcttttatg tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag
    14101 tactggcgct tttgcggagt ccaattagca ccttgcttga attcctgccc tctgcggatc
    14161 aagcccataa cttcttgatc agtaagtgcc tcaacggtca ataaatgctt taagctgata
    14221 cgttctgatt tgatgatcat aatgtttcct ccttagtttt cttcgcttcg tgaagtttct
    14281 ggtaagataa gatttaaaat gatgccaagt acagttgcta atgccattgc cgacaatgta
    14341 aatgtgccta cttcaaagac gagtccgcca attcctacta ctaagataac tgaagcaatc
    14401 aacagatttt tctttttatc aaaatcgatt ttgttctcga ttaaaatctt cattccactt
    14461 gcggcaatta ctccaaataa gataaaactg attcctgaga tgactggtcc agggatactt
    14521 aaaatcaaag cgcttaattt tcctacgaat cctaaaatga ctgcaaatac tgcagctcca
    14581 ccgatcacaa acacgctatg gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa
    14641 cttgttacag gaggacctcc gacaaaggct gccacgattt gtgctaatcc atcacctgtc
    14701 aacgtcttat gaagacccgg ttcttcaaag aaattacgtt tagttagttt attcaatacc
    14761 ataagatggc cgatatgctc tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt
    14821 gctccaagat atagctttgg ttcatattgt acaaagggaa tctcaaagtt aggaacagca
    14881 aaccaggcag cgtctacgat tggcttagta tccacgattc caaacagcaa ggctacgatg
    14941 tatccgctaa taatacctag taaaatcgga atcaatccga gaaaacctct tagccacatg
    15001 ttgaagaaaa ttgttaatcc taatgtgatc agcgctacag caatatactt gaaatcatac
    15061 gtgccctgat tatacatagc atttgttgct gcatttgccg caagtcccaa cccgataacc
    15121 atgatgactg gaccgacgac gattggaggc aagattttat ccagccatgc tgaaccgatc
    15181 tttttgatga tcagcgcaac aatcaaatag actaatccag ttgtgactgc tccttgtgca
    15241 atcgctggat aaccatctgt tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg
    15301 cttcctaagt acgcaggtat ctttccttta gtagtaaata agtacaccaa tgtacctagt
    15361 cctgaactga ctagcgcaat acctggatcg atacctacta agatggggac taacacggtt
    15421 gctccgaaca tcgtgaacaa gtgttgtaaa cttaatccta cccaatgtcc tggggttggg
    15481 cgatcgtgga tatctaacac cacgtcatca ttatgaaatc cttttttctc cgacatgttt
    15541 ctctctcctc taatcttcct ctttcaaaat catgatgcga tcttgtccat caagctcttg
    15601 catctctact aaaatctctt ccgcccgaga tgttggaatg ttcttcccaa cataatctgc
    15661 acggattggc aattctcgat gcccacgatc gaccaggact gctaatgaaa tttttcttgg
    15721 tctgccaaaa tccattactg cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac
    15781 atcatcaatc aaaataactt cttttccttc taaggaaact gggatgtcag aagaatgaag
    15841 ttccggttct tctggatttt cttttttgtc atctcggtat aaagtgatat ccagttctcc
    15901 tacaggaata tcgatatctt ctagctgctt caggcgttcg gcaattcttg atgcaatgta
    15961 gattcctcgt gttttgatac ctaccaatac gatatcttgg atactatggt ttctctcgat
    16021 aatctcataa gtaattcgag tcaatgctcg cttcatggtt acctgatcta ctacttcttt
    16081 tgcttgcatt ttcattcccc ctactttttt gcataaaaaa actcctgccc taagaggaca
    16141 ggaggaatgt ttagactgag cgtatactga tacactgata gctatactat gtcaatgagc
    16201 aatctactta tggcgcacca tacgtatcct tgctcccacc cttcttagcc tcacgggact
    16261 aaacttaaag gaattctatt aagttttatg ctttcgttgt taattactat accgttatct
    16321 aaattaaaat gcaatgtttt ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag
    16381 aggtgcttca aagatcatct tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc
    16441 atggagaaac tgaccattgc ctttcaaagt tttcttcggt ccgtatactg gatctccagc
    16501 taacggataa ccgatatact tcatatgcac acggatctgg tgtgttcttc cggtttctag
    16561 ttttaattca accagagtaa atccttcgaa acgttccaat acagtaaaat gtgtgacagc
    16621 cgacttccca tcttctctta tcgcctgcat tttccggttt tccttcgaac ggccgattgg
    16681 cgcattgatg gttccttttt cgtgaggaat ctctccatgg actaaagcaa catatttgcg
    16741 caaagaggtc ttatctttta attgctgagc taatgattcg tgtgcctggt catttttggc
    16801 aaccattagc agtccggaag tatctttgtc gattcggtga acaatacctg gacgaataac
    16861 gccgttgatc gtagaaagat ccttgatatg gtacatcagc gcattgacca ttgtgccatg
    16921 cgtatgacct gctgaaggat gaacgaccat tccttgaggt ttgtttatca ccgctacatc
    16981 ttcatcttcg taaacgatcg tcaacgggat attttctggg accacatcta atgtttctgg
    17041 ttcggggatc aagacagtaa tctcgtctcc ttgttttact ttgtaatttg ctttgactgg
    17101 ctgtccattg acagaaaccg ctccattttt cagccacagc tgaatttgcg aacgactgta
    17161 atcggtgaat aaattagtca atactttatc gatcctacca gattcatggt gaatcgtcgt
    17221 tttaagttct gtcatttttt tctttgatcc ttttcatcta aaataatata aattaacata
    17281 caaatcactc cacatacaag tgtcacatct gctacgttaa aaatcggaaa gctcataaaa
    17341 tctgtctgga acatatctac tacatagcca agacgaatac ggtcgataaa attgccaatc
    17401 gcaccagcta gaatcaaact caatccaatc atgaaccagc gattaccttt ttcacgattt
    17461 ttaatcatca atgtgagaat cacaacgact gcaatgagtg tcactatagt aaagaaccac
    17521 atcttccctt ctagcatact ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa
    17581 atacctggta taagattttt cgtctctcca agcgctagat tatcagtgat ccaccatttg
    17641 accaactgat ccactcctac tagcaaagcg ctgaatatcc aataaataat taacaaaaaa
    17701 tcaatccttt tctatttgtt tataaatatc acgaggtcgt acgatcccga gcagattacc
    17761 atcgggattc ccatctttag tgataagtac ggcttccaaa gtcccttgct cacgaaacat
    17821 cgcttcaacc tcgtaaatat gtgttttagc tggaacaaaa tgataatttg tgtactttcc
    17881 gttttggatg atcaaatcca acgctcgttt atttgttaaa tcaaccggac ctttttggct
    17941 ttcttttgct agccaaaacc cgatattacg taaagtaatc aatgctacaa attttccttt
    18001 attgtataac ggaaattgtg aatacctttt ttctgctact atctttaaca gttcaagtaa
    18061 cggaagatct tgttcaaaac ctgttacacg ttttgcaaat cgaggcagca cggtttcagg
    18121 ctgaatcagt tcttgttcga tcagctggat tcgcttgact gtccaatcat ttggctctgc
    18181 aatgacaaaa tcgactgcga tccgatcatg gacgatcgca ttccttaact gagcaagctg
    18241 taaaagatca tcttcgtatt ttctgatcat taattgtttt ctttgagcta ggcgtctgac
    18301 aagttcggta aatcccatat ttcgtgcatt tcccatctcg tctctcatcc atttttcgat
    18361 tcgattgaaa ctgattaaaa aaagatcaga gtttgccata attttcctcc ttgatcaaat
    18421 atgcccggtt ttttatatag aaaaagaacg caatcaacac aataactgca aaaaaactga
    18481 aaaataaatt ttgtaggaaa acggccccca acccagttgc tagaaataaa aacggataga
    18541 tttgaagaat caagtaattc attgtgatca cataagcagg ctttgcttct ttcactgtat
    18601 aaacggggct ttttagccaa taaaaggtga cctgcatcgt aaagggcact tccagatcaa
    18661 gcgttatctt ttgttcatga ctgagaacac tgcgtttttc attgttgatt cttagctgaa
    18721 tcgagctacc cataccgtaa aaaccggttt ttcttataaa tgtcgttttc atcataccct
    18781 cctattatgc tttagtatag ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa
    18841 caaaattaat tccctatcgt ctaaaaaaag aagctgtagt caaaatacag ctccttcgat
    18901 ggtagattat aatcaagctt aaaacaatcc caacacttct ccgtcatttg tcacgtccat
    18961 attcaatgca gcaggttttt taggcaaacc aggcatcgtc ataacatctc cagtcaaggc
    19021 tactaaaaat cctgccccta attttgggat gatttcgcgg atcgtgatgg tgaaatcttc
    19081 cggtgcacct agcagtgaag gatcatcaga aaatgaatac tgtgtttttg ccatacaaat
    19141 cggcagtttg tcccatccat tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct
    19201 gaaaacaaca ccatttcctc cgtaaatttt cttgacgatt gtttctgtct tttcttgtat
    19261 cgtttcttct aggcggtata atggcttgta attagcttct tttctatcaa tcaatcgtac
    19321 tacagctttt gctaaatcaa cgccgccttc tgctccatct gcccatacac tcgctctctt
    19381 ggcaagaatc ccctgatttt cacacaagtg ctccaacagt gtgagttcat tgtctgtatc
    19441 tgttacgaat tcattgattg cgacgatcac aggtagctga tattgttcca tattacgtat
    19501 atggcgcttt aaatttgcaa aacctacttt caaagcatca agattctcat ttttaagttc
    19561 atcttttttc attccgccat gcattttcaa tgcacggaca gtggcgacaa tcaccacagc
    19621 atccggcgct tttttcaagt taggtacttt gatatctaaa aatttctctc cgccaagatc
    19681 tgcaccaaat cctgcctctg tgacaacgta atctgctaat ctcaatgctg tcttggtagc
    19741 taaaatacta ttgcagccat gagcgatatt ggcaaacggt cccccgtgga caaaagctgg
    19801 tgttccgtaa attgtttgta caagattcgg cttgattgca tctttcagca gtaatgccag
    19861 agcgccttct gctttcaagt ctccagcagt cacaggctga cggtcaaatg tatagccgac
    19921 aacgatcctt gcaagacgag cctttaaatc ttcaaggtct gccgcaagac acaagatagc
    19981 catgatttcg ctggcaactg tgatatcaaa cccatcttct cttggaacac cttggattgg
    20041 cccacccaag ccaacaatta ctttacgcag ttcacgatca ttgagatcga ctacccgttt
    20101 ccaaatcact cggcgggcat cgatgttcaa ttcatttcct tgatgaatat gattatctaa
    20161 aagagcagat aatgcattat tagctgttgt gatagcatgc atgtcaccag taaaatgcaa
    20221 gttgatatct tccataggca atacctgtgc atatcctccg cctgcagcac cacctttgat
    20281 tcccatcaca ggaccaagtg agggttctcg caatgcaata acagattttt tattgatttt
    20341 attgagcgcg tctcctaacc ctacagtgat cgttgacttt ccttcgccag ctggtgttgg
    20401 attgatggaa gtaactaaga tcaaatgccc ctcagcgtta tcctttgatc gattgattcc
    20461 agaaaaattg attttcgcct tatatttccc atacagttcc aaatcgtctt ccttaagatc
    20521 taatttacta gccacttcaa caatcggttc tagctctgtt tcttgtgaaa tttgcagatc
    20581 attcttcatt tgattacccc ttttcacgaa ctttattcaa caaaatatct tctttgttaa
    20641 gatatgccaa ttataccttt ttttattcag aaaaaaaaca taaaattaca ggtttttaat
    20701 ttatttgacg tacttataaa tactctttat aatgaaggct atgatcagac tttagaaagg
    20761 tcgtgagtgc tttgaccaca ggaattgtaa aatggtttga caacaaaaaa ggatacggct
    20821 ttatcagtta tgatgacaca gaagaaattt ttgtacattt tactgcgatt gaagaagagg
    20881 gattcaaaac tttggaagaa aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag
    20941 gaacacaagc tgcccatgtc aagaaaggca acgttcagac taccgatact agtatgcatg
    21001 aatagataaa caaatagagt gagacgctaa acgggatgaa ccgtacgtct cactcttttc
    21061 ttttcctgta aaaattcgtt tttttaggtt ctgattttta aaattagctt tttctttttt
    21121 tcgtataagg ataaggaatt gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa
    21181 ggactacttg atcttttttt cgcatcatga aaacagatgg ggctttctca tcttgcaaca
    21241 tcatttgcac cccagtatta gttggcgtca aagatcgaat ctgtccgaca gatatttctc
    21301 ggtctttcca aaaagcagca taacgtataa tcacagagtt tgattttaaa ataaaagaac
    21361 gccgcaaacc tagaaataga aaaacaagaa aaatacctaa aacaaaattg cttttccaat
    21421 aaggacctgt gttttctaaa gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg
    21481 accaatagat gatcgtatgt gccagttcag gctgccatcg aatatttttt ttcattctct
    21541 aacctctcac cgattcagta atgatataat cttaccataa cattagtttg aacaaaaagg
    21601 agaatctctt tttatgttaa aagtatacat tgatgcctct acaaaaggca accctggtcc
    21661 tagtggaggt ggaatcttga ttgtctatca aggtaaacaa gaacagctat ctatcccgct
    21721 ttctataggg acaaaccatc aagcagagtt cgaagtcttt ttaaaaactt tagaatttct
    21781 aaagaaaaac gacttacaga atgaaacgat tttctgtttt tcagacagca aaacactagt
    21841 ttctacggtc gacaaaaacg caacgaagaa cgagctcttc cttccctacc ttcaaagaat
    21901 ccaagaactt ctatccgaat tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa
    21961 taaaggagca gataatcttg cacgtcaggc tctccataaa caattaaaag aaaaaaagac
    22021 tctttaaatt aaaaacttta aggagtcttt ttatagtttc tcatttaata taaaagataa
    22081 ttgtgatcaa acgtaatttc caattggtaa tctccgattt tttcttctat ttgactacgt
    22141 aagattcttt ctagttctgt atcaacaaac tggaatttct cagggaccac tacatcaaat
    22201 tgcagcgttt ttccttgatg aattactcta aaatcatgaa tattaagccc tgtacctgtt
    22261 tcaagaagaa tttgctttaa ctgccgcaag atattatagt atttttcgtt atcaacaggc
    22321 attggatcca agtgacagac aagttccaca tccaaagact ttttaaagtc tttttctatc
    22381 agatcaataa tcttatgagc cacattcaaa ttcattgatt catctacttc gatgtggact
    22441 gatgcaaagc gtttattggg tccatagtta tgcaccaata aatcatgata gccaagtata
    22501 tcatcgcaag catttaaatg ctcttccatt tcttcaatct ctgctggcgt tgggcgactt
    22561 cctaatagtt cgtatacaaa atttcgaagc atcgtcaatc cactataaag gatatataat
    22621 gctaaagcaa aacctatata tccatcaata cgccatccag ttacccattc gattgctgct
    22681 gaaagcaata cagccagtgt agtaaagaca tcattataac tatctgttgc tgtagctctc
    22741 aatgtttcag aatcaatgga ttgcgcaata acctgataca ttttgccttg tgcaaatttc
    22801 aaaaagatgg aaatgattag gacaaaaaag acgatgggtg ttaaagagac attttctgga
    22861 tggatgattt tttcagcaga agactctaaa aattgaaatc ctacataagt aatgatgatc
    22921 gaaacaaata acccactgat atattcgaag cgttcgtgtc cataaggatg ttcctgatct
    22981 gctggctttg cagcaatttt aaatccgatc aaagtcaata tggatgaagc agtatctgat
    23041 agactgttca ctgcatcagc cataatggag acacttcctg aaaaaagccc gatcattagc
    23101 ttagagacaa acaagatcag attcgtaacc aatcctaaga tacccgctag tactcctttt
    23161 tttgtccgct gttgcggtat ttgtttcttt ttttcttctt gtttcttagc caagtttttt
    23221 accccttcta gtttgcttgt tgctcaggta aaaagatgga gaggacgatt gttttcctct
    23281 cctctcttcc tttttctatc tatcgtgcgt cctattacta ttaaaagatg gtcattattc
    23341 tggtttcaac ggtgttgctg caaataacgc ttttgctagt aatggtgtta tgattcccgc
    23401 acctattgct tctggtatcc cattgattgc tgcaacccct gctaaggttt taaataaaag
    23461 agcagagtct acaccatagg catctgccgt tgcacctgta taaaataatc ccatgaatcc
    23521 taacactaag actgtattga ccaatgaacc cagtacccct gcaaaggctg agctgatcgt
    23581 tattgattta tttttttgat aaatccagtg gaaaaccaag cctgctacta agccaacaag
    23641 tactctggga actaccgaga taatcggatt cgtgaacact aatgtatcaa atggcgttgt
    23701 tggcatggca taggcacgaa caattgtagc gattccccaa aacaagccaa tcaccatgcc
    23761 atcttttggg cctaatacta ctgcagcaat aattactgtg atatggataa ttgttaaact
    23821 aatgaatcct aatggaataa accccagcca aggaaccatc gcttgtacga tgatgattgc
    23881 taacaagatc gcacgtaaga ctagtcgaaa ggtcttattt ctactattca ttttttcacc
    23941 cctttttatt tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg
    24001 aatccgacca tttgattaga tgcgccatcc aattgaaaaa agtatgaggg caagacttgt
    24061 ttgtcttcgc ccccataatc atgtttcgtt caatgtttga acttttgttt ttctgtcaaa
    24121 cgtaaacgcc ctattgttgc gcaacccatt ctttcgcagc tttaagtgca tctggaatac
    24181 cagcaggatt tttcccgcca gcttgtgcca tgtctggacg tccaccaccg ccaccgccta
    24241 ctttcggtgc gattgcttta atcaagtcgc cagcttttag tccagcttca tttgattctt
    24301 tcgtcattgc cgctagcaga ctgacttttc cttcttgttc gtttgctaaa acaagaatat
    24361 ctgaaatatt tttttgtttc cattggtcag ctaattgacg cagctgattc atatctttca
    24421 cttttacttg tgctgcaatg attgtttttc cattgatcgt ttctggatct ttgaatacat
    24481 caccagcttg ttgattcgct aacttgctag ctaattgttc attttccttt tgtagctctc
    24541 ttacttgctg ttgcaattgt tccgcttttg aaacaacttc tttcaattga ggtgatttaa
    24601 caattgttgc tacttgtttt agttgttttt cttcttcttc aagtagttcg tacgcttctt
    24661 tacttgttac agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt
    24721 tgaagattcc gatgttttct gtatttgcta catgtgtgcc tccgcacagt tcgattgacc
    24781 agccgccaat atttactact cgaacgtcat gtccgtattt ttcaccaaac aaagccattg
    24841 cacccatgtt tttggctgta tcgatatctg tttcaattgt ttcaactggg atagcttccc
    24901 agattttttc gttgacgatt ctttccattt cagctaattc atctgaagtg atttgaccaa
    24961 agtgggtaaa gtcgaatcgt aaatgtccag gagcgactaa agaaccagct tgatttgcat
    25021 gctcgccaag tacttctttc aatgccttgt gcagtagatg ggttgctgta tggtttttga
    25081 tgatccgatt gcgcatcgat tgatcgatca atagatgata ggtttgtcct tcactcattg
    25141 gtgcttttac ttctacggtg tgcataaatt gaccattagg cgctttttga acatctgtaa
    25201 caactgctac gatttcgcct acttcattca tgatccatcc ttgatccgct acttgcccac
    25261 ccatttctgc atagaaaggt gtttgatcaa agatcatttg agcttcacct tctgcgactt
    25321 cattcaaaag agactcacct ttaacgatga ccaataattg actatctgat tcttgttctg
    25381 tgtatccaat gaatttactt tctactttga tatctgtcaa taaagcagat tgtaccccca
    25441 ttgatttttc agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt
    25501 caaaaccagc atgatcaact ttcagtcctt catcttctgc cacctcttcc gttaattcta
    25561 ccgggaagcc atatgtgtcg tatagtttga agatgtcttt tcctgctaga gtcgtcccgt
    25621 tttcgttttt tacttcttgg atcacttgat ttaagatttc caaaccttca ttgattgttt
    25681 cattgaaacg ttcttcttct gtacggataa ctttttcaat aaattctttt tgttgaagta
    25741 cttcaggata ataacttacc atgatttccc caacaacagg gactaaacga tataagaatg
    25801 cttgttcaat acctaatttt ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga
    25861 cataccctct cccttcattc gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc
    25921 gaatatgatc tgcgattact ttgtaagaaa tatctgttac tgggttttca ccatatttta
    25981 cttgtttgct tagcttttct acggcgtgga taataggcat aaacaagtct gtctcaaagt
    26041 tcgttgacgc atcttgaacg attgagacca tgcgttccaa tcccatcccc gtatcaatgt
    26101 ttttatgagg aagtggttca aatgtgttat caggcttatg gttgaattct gaaaatacta
    26161 agttccagat ttctaaataa cgttcatttt ctcctcctgg atagttttca ggatcgtctt
    26221 ctgctaaatc gttataagct tctccacggt catagaaaat ttccgtatcc ggaccacatg
    26281 ggcctgcacc gatatcccag aagttgtctt ctacatcaat aatgtgatct agtggcaggc
    26341 caacttcttc atgccagatg cgttttgctt ctgtatcttt tggataaact gttacataaa
    26401 gtttttctgg atcgaatgcc atccattttg gatcagtcaa aaattcccat gcccagtgga
    26461 tggcttcatt tttgaaatag tcaccaatcg aaaaattacc cagcatctca aacatcgtat
    26521 ggtgccgtgc tgttttaccg acattttcga tatcatttgt tcgaatagat ttttgggcat
    26581 ttgtgattct tggattttct ggcacaactg aaccatcaaa atattttttc aatgttgcaa
    26641 caccagagtt gatccacaat aaggttggat cgttcacggg gatcaaagac gcactaggtt
    26701 caatcgtatg tcctttagat tggaagaaat caagaaacat ttgtcgaact tgtgcactac
    26761 ttaattgttt catgtcatta cctgctttct ataaattaaa tacaaaaaaa caccatcaca
    26821 gccaaggacg aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc attacctctt
    26881 aaactcgata acgctgagct gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt
    26941 gattagtgac tattcttttt tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa
    27001 tcatatcctt ttcgtactta attagtatac gcattctatt gatcactgtc aatatctcaa
    27061 tttttatttt catttccttt tggtcatact ccacgatttt tggtacgata agaataaagg
    27121 aatcaataca tctctgctat tcatgacaag gaggaatatg atgaaaaaaa tagttggagc
    27181 ggatgaatgg atacgcgcag ctagctgctt tttgagatat caagtttttg tattagaaca
    27241 agggattcct gcagaactag aattcgacgg aaaagatatc ccgcaacaaa cctattatct
    27301 atttcttgat caaaaaagac cagtagctac tttgcgttat gaaaagaaaa cacctacttg
    27361 cttcaagccg gaacgatttt gcgtagcaaa agaataccga ggaaaagggt taggcggtca
    27421 gttgcttgaa gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta gttatgtagt
    27481 agctgaaatg acggctgtag atttttatga aaaacaaggg tatcatactt gcacagaacc
    27541 ttttattgaa gatgaagtac tttgtatcgg aatgaaaaaa agtctagctt aaaaacaaga
    27601 gattgtgaca cgccttttgt cacaacctct tgttttttat ttatgtgaat ttttcttatt
    27661 tttacgagca gtacggcttt gctgtctgcg ttcgattttg cgttcttttt tatttttttc
    27721 tgcaattgcc caatcgattt ttttcttata accaggcttg atctttttct ttttcttctt
    27781 cacaagacca atcagcgtgg gatctaatac atcttttgat ttttctcgtt tggcacgtct
    27841 attacgatcg tatgttttga cgatttcccc gtttttgatt tcttttggtt caaatgagac
    27901 gcccaatttt tctatcgcat caatggcttg ctcatcattt ggcgaatata acgtaatggc
    27961 cgttcctttt aagccattac gtccagtacg tccgacacga tggataaaga agtctagatc
    28021 ttctggaatt tctgcattaa taacatgaga gacaccttcg atatcgatcc ctctagcagc
    28081 taaatcagtc gctacaacat attgatactc cagattctgt acttgtcgca tcactcgttt
    28141 tctctctctt ggggtgatgt ctccatgaat ctttgctact ttcaagcctt ggtttttcaa
    28201 gtaatcggca atttcatcca catgctgttt tgtattggca aatacgattg ctaaataagg
    28261 atgaccaatt gtcagcagtt gataaatcat ttgattctca tctttacctt ttgttgaaat
    28321 cagccaattt tcaatgtctt cagaaataac tgcttttggt ttgatttctt caatgattgg
    28381 attttccatg tattttcgta agaatggacg aagtttttct ggaatagtgg cagaaaaaac
    28441 taaaaactgc agcttttctg gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc
    28501 catatccaat gtcatatccg cttcatcgac aacaaatgca aatgcggtgt gaatcttcag
    28561 cgcttgttca ttcatcatgt ctaatattct accaggcgtg cctactacaa cctgtggctg
    28621 ttggtgatgc agacgattta attgacgctg tttgtctgta ccgccgacaa aattcgagac
    28681 acggatctcc ggtgtagaaa atttagcgat ttgtgtagct gcttcataga tctgtgcagc
    28741 tagttcccta cttggagctg taataacaac ttgtacttcc tctttttctg cattgatttt
    28801 ttcaaacaaa ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag attgcccaat
    28861 gatatttttt ccttttgcaa ttaaaggaat caaacgttcc tggacctctg tgggctgttt
    28921 gaagcccttt gcggttaacg cttcattgat aaacggtttg aagcggaata tatcaaaatt
    28981 attcattcgt gcacctcttc tctttaaatt atcacgactt tggcattata acacagacaa
    29041 agcgtttttg ctcagctaat ttcgagaatc atcatccttt gtttggccaa tcgtgctaac
    29101 agtatatgtg tgactagctt tttcataaaa cagaatccgt ttttttctca aaaatgatag
    29161 caagatattt aaaataaaaa taccttgtag taacaagaca ccgagagaaa tgaatactgc
    29221 actcatctgc aacaccgtat tcgttgtcga attcatgatt tgctgaagca actgccccaa
    29281 ccagcttaaa ccataaaaaa gaaagtacag atacccgtag cggatcatca atgcttttaa
    29341 agaaattcgt tcgcggtctt cttccaccaa acggatacgc acaatttttt tgccaagtgt
    29401 ccatccattg gttataaacg gtacaatcat aaagtatacc cctacttcta ataaaagcca
    29461 ccagatatga gaagtaactg cccctgaatc gaacgcaatt ttaaagaaaa tcgccgctac
    29521 caaattaata agagataaaa atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa
    29581 tgagacagtc tttccttttt cataagaaag ttcatccatt cgtcttcttg taggaagcat
    29641 aaatgtaaat aacggcgtaa cgacaaaacc aatgattcca cccaatgtat ttgtcactaa
    29701 atcattcaca tcaaataagc ggtatggtct tgggtagata aaataaagtc cacttagctg
    29761 cgtcaattca aaaaatagag aaaggcagaa actagcaatc atcgttttca taaaagaaag
    29821 ccggaaataa tatcttaaat aaatgccaaa cggtatgagt aataaaacat taaaagctgg
    29881 ttccaaaaat acactctgct tcattgccgg caggtaggta ctaggttcag acaagaccaa
    29941 taccgtgttg tctctgaaat gttgccatga agcaaataaa tgcagctcca tctttggccc
    30001 tgtcatttgg gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa aatacgcaca
    30061 caataaataa aaaacgaaag aatacaaaat aactgccctt gttactagaa atgttccata
    30121 ctttcgatat tcataaataa aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac
    30181 aatcagcgca aaacgaatcg gttcgatata acttgccata tgactcctcc ttgattcttt
    30241 gtatctctct tatcttagca gtcctttgct gtttcaagca agtaacgagg ctattatttc
    30301 tcatttcttt caaagtaaag taagataaac acaaaggggg tgtcgagatg ctgaatatgt
    30361 actttgtttt tggcgtacct atttttttac tgttccttta tgctacgatc gcttatgtaa
    30421 gaaaaagaac tacaattcat tatttaggat ttattctcct gattatttcg ggtttcatgc
    30481 tagtcttcaa tcttcagact tggcagcaag cacttcttga aatggataaa atgactccac
    30541 atgctttgtc taaagtgctt ggctaccctg tctatctgat ttggcttccc attttcattt
    30601 ctggttgctt ggttttatta aatatttatc gcggcgtacg ccgaatagtt cagttacgca
    30661 aatcaaagta aaagacagaa atcggttgag cccgatttct gtcttttact ttgctatttc
    30721 atcgtagaca aaacgacatt tacgtgatca aaattaacga gtacttttga catttcttcc
    30781 acgtactcct catttaaata aaatattttg tttggcagag aagtgcagtt ctaataaaat
    30841 tcgaatgttt tagctatcta cgttttaaat agagataaat cgggatacca gcaagtgtca
    30901 atactatccc catcgaagct aaaaaggtct gagtgataag cgtgcttgat acgataaaag
    30961 tccctccaat gattgctact agtggaataa acggatacat tggaactttg tatggtcgaa
    31021 acaaatcagg ttctttttta cgaagaagaa tgacaccaac aaagaccatc gtataaaaaa
    31081 tccaaatgac aaagatcagc atatccgtta gtgtatcaaa accaccgatc atcatcatgc
    31141 caatagcgat gactagctct aagatacctg cggctacagg aactttagtt ttatcatgaa
    31201 gtttagcaaa gagcttgcta aaaggcaggt tgttttcttt tgccataaca taagggagac
    31261 gcattccagt aagggtatag ccattgatgg ttccataaac agaaatcaag ataccaactg
    31321 tgaccagtct tccaccgaag ccgccaaaaa tcatttttgc aacgtcactt gccgcattgc
    31381 tgtttcctgc gactccgtcg atagatgcgg ttcttaagaa caccgcattt actaataaat
    31441 aaacgatcat gattccgatg attcctaaag aaattgcttt tggcaaatct ttcgccggct
    31501 ttttcaattc tcccgaaata tttcctacgt gtatccaacc gtcgtatgcg aacatcgttg
    31561 ctagcaagcc cgctcccaag gctgagaaaa atgaaagatt ttctcctgct tggatcggaa
    31621 acagttggaa gtctactcct tcttgtcgga acaaaccgaa aatgacgatc acaaacaaag
    31681 gaataagctt gcagacaagc gtaatcgact gaaaagcccc tccagcttta gaacctaaaa
    31741 aattgatcaa caggatcgat acagcagctg taacagctac gggaacaatc attgactggg
    31801 aaagaccaaa tagattgacg aactgtgtac cgaaaatgat cgacaaggct gctacatttg
    31861 ctggaaaata aatcacgact tgcgcccagc ccaataaaaa agctgctgta tttccgtaaa
    31921 tccgttcgat atatttaatc atccctcctg tttctggaat agcagctgcc agttcagctc
    31981 ctgttagtcc tgcacagaca gaaatcatac caccgagaaa ccaagaaaac atatgcaaac
    32041 tggcggatcc tgtaacttct gctacgcttg cggctttaaa aaatacccct gcaccgatca
    32101 ctgtgcccat aacagtcgat aaagcagtaa aaaatcccat cgtacgtttt aattcatttt
    32161 ctatttttac ttcttttact ggcgtggctt gatattcttc catcccttgt cccctttcat
    32221 accatagcca atcccaaaag tataaaaaac tttttggatg tggttcttgt tttataccgt
    32281 ctctaccaat ttttctttca gtttttccaa catatccttt gtcatcacat ctaagtcgta
    32341 tttcggcgaa aagccccatt cctcttgcgc acaagaaaca tctagtgaat ttggccaaga
    32401 ttctgctatt gactgacgta caggatccac atcataatcc atttcaaaat ctggtatgat
    32461 tttttgaata gatgctttga tctcttccgg gtcaaaagac atcgccgata tattgaaggc
    32521 atttcgatga accaatcgat caggatctgc attcatgagt tttataatgg catcgatcgc
    32581 atctggcata tacatcatat ccattgatgt accttgatca atgaaactag tgtactttcc
    32641 ttctttgatt gctgaataat aaatatcgac tgcgtagtct gtcgtaccgc ctcctggcaa
    32701 ggttgcataa gaaatcaatc cagggaaacg gacaccacga gtatctacgc catatttcac
    32761 ataataataa tcacatagta gttctccagc taccttcgtt accccataca tggttgtagg
    32821 acgctgaaga gtatcttgtg gggtatgatc ttttggagta tttggaccaa acgcgccgat
    32881 tgaacttggc gtaaaaaatt tcagctggaa ttctctagcg acttccaatg cattgaccag
    32941 cccagtcacg ttcaaattcc aggctaattg tggttttgct tctgcaacag ctgacaaaag
    33001 agcagccaaa tgaatcaatg tgtccacttg atattgttct gctatgctgc gcattttttc
    33061 gtaatccatt acatctagga tctcaaagat atcgtttttc tcaatgactt caggcatgcg
    33121 tatatctgtc gcaatgacat tttcactgcc tagttcttga cgtagccgtg tgatcaactc
    33181 tgaaccaatt tgtcctaaac atcctgtcac caatactttt ttcatgatga tcctccctga
    33241 tcttttttac tactatcttc agatgattaa caatatatgc gtgaacatgt tctctaattt
    33301 taaatagacg aaaaaactaa atttagttaa ataatttcca tttcttttcc tattttttga
    33361 taaactgcta ctgcttcatc aagcatttcc ctcgtatgtt ccgcagtcgg catatttctg
    33421 atcctgcctg tcccaagagg cacagttgga taaacgattg gtttagcata aatcccatgt
    33481 tcgatcagct tcttagagaa agtctgagtc actttttcgt ctcccaaaat gactggagta
    33541 ataggtgttt cagacatacc gatgttatag cctacttttt tcaattcttc tttaaaataa
    33601 ttagcatttt cccaaacttt ttcgacaagt tccggatgtt cttgcatcaa agtaatcgaa
    33661 gccaatgcag ctgcagctgc acctggagtt agcgaagtag aaaataggaa tggccgactt
    33721 ctagctttca accagtcgat tagtgttttc gatccagcta cataaccccc aacaacacct
    33781 atagctttcg ataacgtgcc catttgcata tcgattttgt cgcttaatcc aaaatgcttg
    33841 acggttcctg caccttttcc agttacgcct gagccgtgcg catcatcgac gtaggtgatc
    33901 agtccatatt tttcaacaat cggaataatt tccggcaatc gtgcaatgtc tccatccatg
    33961 gagaaaacac catctgtgat atacatgatt tttttatatt tcttactttc tatcgcttcc
    34021 tttgcttttt tctcaaggtc tttcatatct tgatgtttaa ttcggatgat ttttgctccg
    34081 gataagcggc aaccgtcaat aatagatgca tggtttaatt catccgataa gatagcatct
    34141 tcttttgtca ttactgcaga gatagcgccc atattgcagt tgaatccaga ttgaaaggcg
    34201 attgctgctt ctgtcccttt gaattctgcg attttttctt ctaattgctg atggattttc
    34261 agcgaaccat tgatcgttcg gactgctcct gctcctacac cataagtttc ggttgcttca
    34321 atacatgctt ttttcaattc ttcgcgattg gcaaatccaa gataattgtt ggatgctaga
    34381 ttgatcatct tttttccgtc tacgatgata caagcaccat tttcactttc tagtatgtcg
    34441 atcgtattgt ataacccctt ttctttcagt tgatccaatt ctttctctaa atacattgtt
    34501 agttctttgg aagacattgt ctccacctcc atattcatct gtcttgcaga acataccaaa
    34561 catatatcgt gtcattcatc tacaaagcaa gtatgctata tttttgataa cgcttacact
    34621 gattatgaga attatttaac cacatttttt atttacagta tctttttact ttcaaatgac
    34681 ttatatagta tgaacaatta ccttttagat aatgatctat tttactctac ctgggcagat
    34741 aaatataaca caaccattga tagaagccct ttcgttaaaa tcaattgtat tctgaatatt
    34801 taatttattt attgcataga ccaataaaaa tacataaaat ttccatcttc gtttatcagc
    34861 aatttttaat tttactctct gaatatttct taaaagtttt acttgttgaa ttcgaaaaaa
    34921 ggactgtgac aaaagtcttg tcacagtcct attcttccga a
//
LOCUS       DASJSZ010000020        34525 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.020, whole genome shotgun sequence.
ACCESSION   DASJSZ010000020 DASJSZ010000000
VERSION     DASJSZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.020"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(176..1462)
                     /locus_tag="U4B03_000942"
     CDS             complement(176..1462)
                     /locus_tag="U4B03_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN2067813.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="U4B03_000943"
     CDS             complement(1455..2723)
                     /locus_tag="U4B03_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN2067814.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="U4B03_000944"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="U4B03_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEN2067815.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="U4B03_000945"
     CDS             complement(3495..3941)
                     /locus_tag="U4B03_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2067816.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="U4B03_000946"
     CDS             complement(4034..4279)
                     /locus_tag="U4B03_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEN2067817.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="U4B03_000947"
     CDS             complement(4394..4972)
                     /locus_tag="U4B03_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2067818.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="U4B03_000948"
     CDS             complement(5049..5579)
                     /locus_tag="U4B03_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEN2067819.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="U4B03_000949"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="U4B03_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEN2067820.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="U4B03_000950"
     CDS             complement(6436..6831)
                     /locus_tag="U4B03_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEN2067821.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /locus_tag="U4B03_000951"
     CDS             complement(6828..8240)
                     /locus_tag="U4B03_000951"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEN2067822.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="U4B03_000952"
     CDS             complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="U4B03_000952"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEN2067823.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8859..9098)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9095..10555)
                     /locus_tag="U4B03_000953"
     CDS             complement(9095..10555)
                     /locus_tag="U4B03_000953"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEN2067824.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="U4B03_000954"
     CDS             complement(10725..11882)
                     /locus_tag="U4B03_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEN2067825.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="U4B03_000955"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="U4B03_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEN2067826.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="U4B03_000956"
     CDS             complement(13349..13876)
                     /locus_tag="U4B03_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEN2067827.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14106..14912
                     /gene="proB"
                     /locus_tag="U4B03_000957"
     CDS             14106..14912
                     /gene="proB"
                     /locus_tag="U4B03_000957"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEN2067828.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14909..16156
                     /locus_tag="U4B03_000958"
     CDS             14909..16156
                     /locus_tag="U4B03_000958"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEN2067829.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16232..16576
                     /locus_tag="U4B03_000959"
     CDS             16232..16576
                     /locus_tag="U4B03_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067830.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16693..17019)
                     /locus_tag="U4B03_000960"
     CDS             complement(16693..17019)
                     /locus_tag="U4B03_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067831.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17634..18836)
                     /locus_tag="U4B03_000961"
     CDS             complement(17634..18836)
                     /locus_tag="U4B03_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2067832.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            18953..19468
                     /locus_tag="U4B03_000962"
     CDS             18953..19468
                     /locus_tag="U4B03_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2067833.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19667..20113)
                     /locus_tag="U4B03_000963"
     CDS             complement(19667..20113)
                     /locus_tag="U4B03_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN2067834.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(20160..21638)
                     /gene="msr(C)"
                     /locus_tag="U4B03_000964"
     CDS             complement(20160..21638)
                     /gene="msr(C)"
                     /locus_tag="U4B03_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN2067835.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(21992..22621)
                     /locus_tag="U4B03_000965"
     CDS             complement(21992..22621)
                     /locus_tag="U4B03_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2067836.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(22605..23069)
                     /locus_tag="U4B03_000966"
     CDS             complement(22605..23069)
                     /locus_tag="U4B03_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2067837.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23556..24824)
                     /locus_tag="U4B03_000967"
     CDS             complement(23556..24824)
                     /locus_tag="U4B03_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2067838.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(25055..25792)
                     /locus_tag="U4B03_000968"
     CDS             complement(25055..25792)
                     /locus_tag="U4B03_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2067839.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25859..26428)
                     /locus_tag="U4B03_000969"
     CDS             complement(25859..26428)
                     /locus_tag="U4B03_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN2067840.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            26556..27428
                     /locus_tag="U4B03_000970"
     CDS             26556..27428
                     /locus_tag="U4B03_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2067841.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            27770..28642
                     /locus_tag="U4B03_000971"
     CDS             27770..28642
                     /locus_tag="U4B03_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2067842.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            28639..30597
                     /locus_tag="U4B03_000972"
     CDS             28639..30597
                     /locus_tag="U4B03_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2067843.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(30929..31780)
                     /locus_tag="U4B03_000973"
     CDS             complement(30929..31780)
                     /locus_tag="U4B03_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2067844.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            31903..32370
                     /locus_tag="U4B03_000974"
     CDS             31903..32370
                     /locus_tag="U4B03_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEN2067845.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(32633..33115)
                     /locus_tag="U4B03_000975"
     CDS             complement(32633..33115)
                     /locus_tag="U4B03_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEN2067846.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(33215..34078)
                     /locus_tag="U4B03_000976"
     CDS             complement(33215..34078)
                     /locus_tag="U4B03_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN2067847.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         9864 a         7270 c         6217 g        11174 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tctccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aattgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataac gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttggatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcggt tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    13921 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    13981 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    14041 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    14101 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    14161 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    14221 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    14281 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    14341 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    14401 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    14461 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    14521 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    14581 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    14641 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    14701 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    14761 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    14821 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    14881 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    14941 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    15001 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    15061 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    15121 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    15181 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    15241 cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    15301 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    15361 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    15421 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    15481 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    15541 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    15601 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    15661 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    15721 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    15781 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    15841 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    15901 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    15961 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    16021 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    16081 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    16141 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    16201 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    16261 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    16321 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    16381 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    16441 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    16501 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    16561 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    16621 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    16681 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    16741 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    16801 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    16861 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    16921 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    16981 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    17041 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    17101 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    17161 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    17221 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaat
    17281 cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt
    17341 tgtttctcta atacttagga taaaacgctg attttacaac ctctgttaaa cggtgttcaa
    17401 tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt
    17461 tgtttctcta atactcagga caaaacgctg attttacaac ctcctgataa acggtgttca
    17521 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagaag ctatttttgt
    17581 ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctgat ttcttattct
    17641 tcttcatttg tagcaaaata attacggtga taaatgatac aacatacact gataagaaca
    17701 atggcacctg caaaataaaa cgcatagctt tcaccaatat gttcaccaat cacacctgca
    17761 ataaatggtg ctaaagctcc accgaaaaag cggacaaaac tatagctgga cgaagcaacg
    17821 tttcgttgca gacctggtat ttccatagca atcgtcgtca acagtgtatt gataattcct
    17881 tggaaaaatc ctgctaacac aatgctgatc ggtacaagga ctggcataga agttccagct
    17941 cctaataaca gcaaacaaat aatgaataag acaaatgctg ctaaaattgt ccgataagtc
    18001 gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag caatagccag aagcacaccc
    18061 cagccaaaaa atacaaaacc gacttctaat tcagaatagc caatcaataa aaatggcgca
    18121 tacgcaagta aagtaaagaa tccgaaatta tataacaatg cgattagtcc aactgaacga
    18181 agtttatgat tgcttaacgc tttgacacct tcccaaaggc ctacttttct ctttggtttc
    18241 tcagtttcag gtaaaagtac cgctagggct ataaaaccgc aaaacatcaa aaatgctacc
    18301 ccaaaaaatg gataacgcca tgaaccactg cccaagaccc caccgactag agggccaaca
    18361 gccattccgc agccaagtgc tgcttcatac atcatgattg ctttttctgt gttaccaact
    18421 aaaatactga cgatcgttgc taaagcagta gagacaaata aagcattccc gacaccccag
    18481 ccggcacgta acccaatcag cattccgata ttcccagata atccgccaag accagcgaat
    18541 acaacgatga taaacaatcc tgtcatcaat gtttttttca aaccgatccg actagataag
    18601 aaacctgtaa atagcatcac cacaccagtg actaacatgt aactagtaaa taacagggtc
    18661 gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt ttaagattgg gtctactaga
    18721 cccactccca taaaagcgat catacaggta gctgctacaa ctagcgctga acttttcccc
    18781 tttgtttctt catttgtttg ttcttgtata tctatttgat tctccaatat ttccatccct
    18841 ttctctctat tagcatcata catgatttat tttacatgtc aatatgcaca tgtaaaacgt
    18901 ggaaacttta ctgtgatata ataaagaaaa taagacgaga ggtaggattt ttatgaatac
    18961 tttagcctat atattattat gcgtgctttt atccaaacca cgttctgggt atgaattgaa
    19021 gcagctgatc acaatttttt gggaagccca tcacagtcag atctatacaa cgcttgccaa
    19081 gttagaccag cagggttatg tgaccgtttt ggacaaagac gagcactctc aaaaaaagat
    19141 ctaccacttg actgatgaag gacgtgctct tgtagaggaa tggatcaaag aggaaactcc
    19201 cgctcctacg caaaaagatg aatttctagc aaaaatctat gctttttcga cgctcgataa
    19261 aacaacagct actggactgc tattcactag agaacaacag ctgaacaaaa tcatgcaaaa
    19321 aaatcaaaaa aaattgaatg acctctcgct agacaagaaa caagaatttg gacgttatgt
    19381 tgtgatccag cgcagaatta tgctttgtca gcaggaactc caatggtgtc agcaagtaag
    19441 agaacaaatg cagcagtttt ttgagtaagg gataaacaga acctttctag aaatatctat
    19501 tgaagagcaa attatcttca ataaaagagc aatacagttg gaaaagaaaa agcgatccga
    19561 acgacaggcc aaaacctatc gttcggatca ctatttttct agaactgcat tcaagagact
    19621 atgacaaaaa gcatgccaag gccttttttc aaataacagt gttccatcat tcattacttt
    19681 gaattgcttt tagcacactt cttgaaagcg ggccaacgat gatcaactgc aacggtaaag
    19741 cggcaatcac attcattttc caagcattca tataagcaga gaataattga tcaggtattc
    19801 cttgttccat gatcacaccg aataaagaca tacatgtcac cattcctaaa accatcaagc
    19861 atgaaatagt taagatcatt tggaccttgc tttctggatt tactggtagt ttaaaggcaa
    19921 tttttttcgc cagcacaccg atgatgaaga catccaaaat gaaagccacc acgaatccag
    19981 gcactagacc aatcaacaaa cttgataaat gaagatcttg atgcagccat aaattatacg
    20041 cgctcatccc caagaccatc aaaaaacaca tcattgttgt aaaaattatt ccttcttttt
    20101 tatttgtagg caatctgtac cactccttta aatttgtcac gaaaatcatc gtagcacatt
    20161 taaaaatttc tcgtaagtac tttttttatt ttctgccctt ttatttcata aacttcatca
    20221 gccgtacgct cgacaaagta agaatcatga gaagtaaaca aaatcgttcc cggatatatt
    20281 tgcattagct tctctaaagc ttcgatcgtt gccatatcaa taaaattagt cggttcatct
    20341 aacagcaaca cattacttgg cttagtaaaa agcaaagcaa tcgtcagacg ggtcgcttct
    20401 cccccactta atgtacataa cggtttcgtc aaagtttcct gagcaaaccc taagtgattc
    20461 agcaaactac ggacaagcga ttctgaaaac tctgtttgtc tcatgacaaa acgaatgatg
    20521 gattcttcag atgtcatttg ataacccttt tgctgatata cttgaaagct tactttcgga
    20581 gagagaagga ttccttctcc ttgtttgcgg atatgttcta ataaggttgt cttacctgaa
    20641 ccattttcac cgacaatcgc gattttttta cctaaagaaa actgaaaatc accatttacc
    20701 agtaatgttc tcccacttct ttccaattgg acattttgtc ccatgatggg atagcggctg
    20761 tggagttcaa gagattttgg tactggaaaa cggatttgtt tcatctgctg cggcttggtt
    20821 gtttctccta ttttttggag tcttctctct aatgttttcg cattcttttg gatggctttt
    20881 tgtacactgt ctttttgttt agaggaagac aaccgatcca gtctgatttg ttgctttttc
    20941 tttttactgg acactttttc caacttttcc gcttgttttt tcttttcttg gatcgatttt
    21001 tccaatcgtt ttttctcttt catgatcgac tctgcttctc gtagctgagt ctttttctcc
    21061 aattcctttt gagtgagata ggcactataa ttccccgaaa actctcggat ttctccgtct
    21121 tttacttccc agattttcga tgctagctca tctagaaaat agcgatcatg actaacaaaa
    21181 accagcgtac cataataata acgaagctct tcaatcagtc gatcaatact ttttctatct
    21241 agatgggttg ttggctcgtc tagaagcatc ccttgttcat aagaagaaag aagacgtatc
    21301 aactcgatct ttcgttcttc ccctccgctc catttccgct ctgaagtttc ttccagtctt
    21361 aactggaaat gactggctaa ttccaaattt aggtcctttg cttcagcgag ataggttaat
    21421 tgaggcaggt agttgaaggt gatctctgtt tggattttcc catgatcggg aaaaagttca
    21481 ccggctatta gtttgagcaa agtcgattta ccgacaccat tttctcctat gatccctata
    21541 cgatcttgct gatagacacg aagagaatca atagatagtt ctagctggtt gccaaagctg
    21601 acttgtagat ttgttatatt tactgctaaa ttttccatat gttttacact cctatttcat
    21661 gtaaaacaac aattatcgat actaaaaaag agcagaaaaa tctgtttgca aaaaaactgc
    21721 atcacatctt ttcctactct gaacgaaata actatgacta aatcaccaaa tctgtcatag
    21781 ctttcaccta ttctactgtc gttcgaaaaa atggacatac tccatccatg aaattgttgt
    21841 ttatacatgt cttttgaatt tatttagttg ttattcaaaa gttcgaaacg taatttcatc
    21901 gatgcagtca tttttccgac ctcctttttt attaggttac cacacacgat tcaaaaaaag
    21961 caatagctac tgaatagtaa ctgaaaacct tttattcgaa catagtgatc ttttctggtg
    22021 ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc tattgataaa ataataactg
    22081 gaacataacg gtctttatcc aaaccaaatg cctctgccaa ctgatctgct tcgaatccac
    22141 caattggatt tgtttcatag ccatgatcac gcgcaactaa catgaattgc atagccgcta
    22201 agctagaatc gactttgact acatcgttca tttcttctct tgaaaaactc ttgtaataag
    22261 gaatgatcgc agctaattgt tgatctctga cttcttttgg catcttccct gattcgtaag
    22321 cttggttgta gatttcttcg ccatattcgt aacattgcat atcgccaaaa atcatgacca
    22381 ttgcagaaga agtatcattt tgtctcgtat tgaaacggat caaaggacgc aagatttctt
    22441 ttgcttcctc gctttcagcg accacaaagc gccatggttt catattgaca gaagaaggtg
    22501 cagtcgtcgc ttctttgatc atttccagca tttcttcgtg tgggattttg tatgtttcat
    22561 catatacgcg aacggatctt ctaccagtca taattgttga aaaatcattt ttttgttcca
    22621 ttattattaa cctctttcgt ttctgttgtt atattgcaaa gttgggtatt caatttatct
    22681 aaaagtgtta gaaattgttt catttctaca tcgctgaaag ccggggatag cacggaacct
    22741 gtatctttat ggtccatttc acaatgcttg attttcttct gggctttttc tgttagcgct
    22801 acatacactt cccggttgtt ctgaggattt ctctgacgcg aaacatagcc atttgcttcc
    22861 aataccttca gatgacgagt aactgctcct tgatcaatct gcaacttttc ttgcaaactg
    22921 ctttgtagca atggattttc ttctttcagt ctcatcatga tctcgtatct agtaagactt
    22981 ataccgatat tttcttcaaa gatctggacg attttttgat cggctaattt gatttgatat
    23041 aaaaggtgac taatctcttc taaagacata atttcctcct ttctaataat tgactcgtca
    23101 acgattgact tgtcaataat agaataaaaa aagaaaaaag tcaaagaatc agctgtgtaa
    23161 taaaaaaaac gctctcaata gaaaagtata gagaaaagaa attttttgta gaatgaacgg
    23221 aaaaaatgat aaagaaagca caacgctaaa accaagatta ttgattctta tcaaactata
    23281 gaagatagct cttctgcagt agataatttt acaccgccaa aaatttccga aatcgccttt
    23341 tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg cttaaacagt aataaaaaag
    23401 ttatgacaag atgttgtcac aacctttttt gagtaaacgg tgttcaaaaa cttgcatctg
    23461 cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt ttttgtttgg ctgttcttac
    23521 tacttgatgc agggcagctt tttcactacc tctttttaat aaccataaaa tcctattcct
    23581 tcatatctcc agcccatcgg tgttagccaa tctctttcct tagcactcaa ggtatagtga
    23641 tgggtaccag ttttttcatt tggattaaat agtcgataaa ttggcttgga accaccagaa
    23701 taaaaactta gaccttcata acgccaaccg atttttgtta aagagttgat ctcagattgt
    23761 gccattgtgt aatgatggtc tccactgtaa ggattgtata aacggtagac aggttgtccg
    23821 ctgtttggtg cttgccaagc aatcccttca tattcccagc ctacagttgg cagataattt
    23881 ttttctccgg cattcaatgt atataaatgt tctcctgtat gacggttata cagacgatac
    23941 atcggagaag ttgaagttgg tgctgtatca tactgtgtca gattataggt ttcgattaca
    24001 ttgttcaatg tagaaccata attaggcgca gtggcataac gacctgttag ccaagccgta
    24061 gcatctttgt aagagttcgt attgcttttc catgcaccgg aatagtagta aacgcctggc
    24121 tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca atgattcttt gtacgaagga
    24181 tattttctaa aagcggcatt caccgtaatc cattgcccat tcacatattc cgaagtaggc
    24241 atcgtaacag attggccatt atagcttcct ttaatcccaa atagattgta attaggggca
    24301 gaagccaaag tactgttccc ccagccactt tcaacgatgg cttgagcaat catgactgat
    24361 gcatataagt cattggcact tgccaaactt tgtgcagttg aggcaatact atcaatgaac
    24421 gcttgttgag aattagcaga tgagcgagca taaaaagcat agccagtata gttttcgaat
    24481 tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc catctgtcaa ttggttcgtg
    24541 atttcttctg cattctctgg attgttctga gcttctgctg atgtactgtc ttcggtttgt
    24601 gtttgttctg ttgtttcagt tgttggttct tcggttgaaa ttgtattttc tgagacagta
    24661 gctgactgca aagtactttc tgtatcgttt tcaattcctg tttctgaaga actcgatgtc
    24721 tgagttgcga cagtttctgt cgattcttca gctagcactg ttgtgttggc aactgctaga
    24781 tttcctaaaa gaataacgct aaataataaa ttctttgctt tcatcttttt tctcctttga
    24841 attatgaaac ttttcctaaa taacagtgta actaaaaaag taaaagcaag tcaatgtcca
    24901 tatttaaata acatttgttt ctttttaata aatttgcttt ttctgcataa aaagacgtaa
    24961 atgaagaata ttcttctcca ttcacgcctt aaattgatgg tcgtttgaat aactatttaa
    25021 caagtaattc tctctggata ttatccgtta tggattatac ttcctgtgtc gtcggacgga
    25081 taatcatgtt gctgacagac atccggtctg gcgtttctat cgcaaatgct acagcagaag
    25141 ctatatcttc agatgttaat ccccattctt tttgcgcttc atgaagttca agagcagcct
    25201 ttttgtcgct gatggtttga tataattcag tttgaacagc tcctggagag atgatcgtag
    25261 atttgatgtt attttgccgt tgctcttgac gtaatccttc catgattgca cggacagcaa
    25321 attttgttcc gcaataaacg gcggaatcag gataaacaac atgtcccgcc acagaatcag
    25381 ttgaaatgat atgcccagat ttttgttttt ccatgattgg aagtacggcg gaaattccat
    25441 ttaacacacc catgatgttg atatcaagca tattttgcca ttcatcacgg tgaccttcaa
    25501 ctaatggagc ggttggcatg attcctgcat tattgtacaa tacatcgatt cgaccgaact
    25561 tttccatcgc taaatcgata actgcttgga cttgtgcaaa atctcttaca tctgctgttt
    25621 gaatatatag ctcagcttct ggtaaagatt cttttattgc ttttaggcga tcttctcggc
    25681 gtgcagctat aactagttta gccccttttt ctgctaatag cttcgttgtt gcttcaccga
    25741 ttccgcttga agcgcccatg atgacaatca ctttttctga taaatcattc attctattct
    25801 cctcctgttt tgaaaaatct cttgcttata ctcactatct cttatttcat cttactgttc
    25861 aaataatccg ttcgacaggt ttgacgattt tggcagggat tcctgcaacg atggtattgt
    25921 ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat ggcattctcg ccaatcgtta
    25981 cgcctggcag aatagttgct cctgctccga tccaagcatt tttcttgatg tggattggct
    26041 ttactgacaa gcctcttcgt tcttttggat cggttagatg gttcaccgtg atgaggttga
    26101 cacgaggacc aatcaatact gaatcttcta aacagatgcc acctaagtca acgaacatca
    26161 cttgacgatt gataaagaca tttttcccta tactaatatg tttgccgaaa tctgtataaa
    26221 aaggcagcag aacagtcacc gtatcatcta ctttgtcact aatgatattt tctagccgtt
    26281 tgcggatatc tgctggttca tggtacccag tattcagcgc tgctaaaggc tcttcgtttt
    26341 ccgcttggat ctgatggatc acttcaaaga gcggatccgt tactcgaatc tctttattga
    26401 cgatttgttt ttcgatgagg cttgtcattt tgctccctcc ttttatcctt actttataac
    26461 ggacggctta tgatgtctaa tacttataaa acatacttaa ctattccttt taactatact
    26521 tagtggtatg cttttttaaa aacaggaggg aaaaaatgga tattcgtata ctgacgtatt
    26581 ttttagcagt agctagagaa aaaaatatca gccgagcagc aaaatctctg catatcacgc
    26641 aacctactct ctcaaaacaa ctgaaagatt tagaagaaga acttggtgtg acacttttca
    26701 ctcgaggctc ccgagagata cagttgacgg attctggtcg atatctttat tcaaaaggaa
    26761 aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt aacaaatgac gcaatcatct
    26821 ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat ctctttcatt tctgatagcc
    26881 ttcattcgtt gatcaaggcg catcctgata tccagtttca tttatatagc gggaatgcag
    26941 atgatatcat agacaaactg gatagcggat tacttgattt tggactcgta atcgaaccga
    27001 caaacaaaca aaaataccag tcatttcgac tgccagccca agatcgttgg ggacttttga
    27061 tacatcggaa tcatccttta gcaaagcaaa cagtcatcca tccgcaggaa ctgaaaactg
    27121 ttcctttgat catttcccgg caaacgtcag tggacagtca actagctgaa tggctaggtt
    27181 tcagcataga tcagttatcg atcgtcggaa catataactt attgtacaat gcttctttga
    27241 tggtcgataa cgggactttt ggtgcattag ctctagatgg gatcatcaat acttatggga
    27301 cggaaaaagt attcatccca ctcgatccgc cattacaagc ttcactgaat ctcatttgga
    27361 aaaaagacca gcctctttct cctgctgctc gtaaattttt agatgtccta acggagaaat
    27421 acagttaagt agagtttttt tccttcgctt tcagcttttt aacaacgaat aagttaagca
    27481 aaagatccaa tgattaaata acataagttt cttaatttgt tccttccttt tatttctgat
    27541 aattccagta aattactata attataaaaa aagaattgac aaaacataac tgtctatata
    27601 aaattgatta cgttaacaat aattcgtttc atgtgactag atcatactag gcatgaaaaa
    27661 tctctgagga acatggtttc cctaggaggt ttttcgtgtc tatttttgtt tggccgcaat
    27721 caactggtct agcaaacgtg aaaaccaaat cttcaaaaag ggtgcgcgca tgaaaatcaa
    27781 aaagatcttt aatcaaaatg cagtattggt agatgacggt ggacaggaaa aagtagctat
    27841 tggaaaaggg attggatttg acagaaaaag aaatgatttg atttttgatc gtgatgtcga
    27901 acggatattt gtcatggaac cagaaggtca aatcaagttg caaaatttac ttaatcagat
    27961 tgatgaacgc tttttatttg cagcagaaac gatcattgat catgcggaaa ctgttttgat
    28021 ggaaaagttg aatgaacatg tattgatcgc tttgaccgac cacattgctt tttctgcgga
    28081 gaacattaat aacggtatcg tcatccgcaa caaactttta agagaaatcg aagtgcttta
    28141 cagtgatgaa ttcagtattg ctcaatgggc agtagagtat ctgacaaaag agcttgggat
    28201 tccatatact tatgacgaag caggatatat cgcaatccat attcacagcg gacgaagcgg
    28261 tcggaacaat aaccatcgca gtatccgtga agtgacgatt gtatctgaca tcatccattt
    28321 agtggaatca gaattggaaa cagatatcca ttcagaacca ttttcactga attattcaag
    28381 actggtgaat catttacgac tgcttctaca acgaacccat gcgcaacaat atgctgtttt
    28441 agacacggaa atcgtagaaa tggtcaaacg aaaataccca gaaagctata agatttctaa
    28501 aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg atcacgaaag aagaactggg
    28561 ttacttggcc atccatatcg agcgtttaag aagcgcaatc gtacaaacaa atgttaacaa
    28621 tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa tgggacgttc ccttcaactt
    28681 ccagttgcgg tcttacctgc tgctgcttta ctagtaggga tcggaaactg gtgggcatct
    28741 tatagtaacg acattgtcgc tcatttttta caagctggcg gtaacgcagt tttaggtcaa
    28801 ttacctttgc tgtttgccgt tgggttagca ctagggatgt cagtggacaa atcaggtgct
    28861 gctgccttgg caggtttggt cgcatttgaa gttccagtaa atgttttaaa aactgattca
    28921 gtagctactt tattgaatat caaaccagaa tcagttgatc catcttttgc tcagatcagt
    28981 aacgtattta ttggtattat tgccggattg attgcggcag ctctttataa tcgtttccac
    29041 gaagcaaaat taccgatggc tttatctttc tttagcggca agcgtctagt gccaatcttg
    29101 gctgcctttg tcatgttgct gatcagtgca gtgctactgc tcgtatggcc tccggtttac
    29161 tctgctttag tttcttttgc taaattcatt ttgagtttag gctggggtgg tgcaggtctt
    29221 tatggcttct tcaaccgttt actgatccct acaggattgc atcaagcaat gaactccgtg
    29281 ttctggtttg acgtagcagg aatcaatgat attggtaaat ttttggcttc tgaaggaaca
    29341 aaaggcgtaa caggaatgta tcaagctgga tttttcccaa tcatgatgtt cgggcttcca
    29401 gcaggagcat atgctattta cagaaatgca cgtccagaaa acaaagcaaa aacagcttcc
    29461 ttgatgttag cagcagcttt tgcttcattc tttactggtg taacagaacc acttgaattc
    29521 tcatttatgt tcgttgcttg gccattatat gtcttgcatg caattttcac tgggatttct
    29581 ttagcagtga gtgcattctt ccattggaca gcaggatttg cctttagtgc cggatttgtc
    29641 gattacttcc tatctttgaa aaatccgatt gcgaatcagc cattgatgtt gatcgtccaa
    29701 ggattagtaa cagcagtgat ttactacttt ggctttaact ttgctatcaa aaaattccat
    29761 ctgatgactc ctggccgcga ggaagcagat ctttcagatg acgatactgc aacgactaat
    29821 tcagacaata aatatgcggc acaagcaagc aaaatctacg ctgcattagg cggagccgac
    29881 aatgtaactt cgatcgataa ctgtactaca cgccttcgtt tgcaagtcaa agatacaggc
    29941 ctgatcgatc aaaataaaat caaagccact ggcgtacctg gtatgaagat catcgacgga
    30001 aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg ttgcagatga gatggcaaaa
    30061 ctacatggtg gtgctgcaac acgacctgca caaacaaaca ccgtggttaa aacagaaaca
    30121 tttgccggtg gaacgatcga acaagatatt tacgctatcg caaatgggaa actgattcca
    30181 atcactgaag tttcagatga tgtattcgcg gaaaaaatga tgggtgacgg ctatgctgta
    30241 ttaccagaaa atggagaaat cttctctcca atcgctggta cgatcacaaa cattttccca
    30301 acgaaacatg ctgtcggaat ccaaacagat gctggtatcg aagtcttgct tcatatgggc
    30361 atcaacactg ttgacttaaa aggcgaacca ttcactttat atgtaaaaga aggtcaaaaa
    30421 gtcgcaagag gtcaattgat tgctttagtt gatcttgccg ctattcaatc tgcaggcaaa
    30481 aacacggata tggtcgttgt attcacaaat ggcgacaaag tccaaagctt agaaatcgaa
    30541 cccgcacgtg atgtaaaagc aaatgataaa atcggctctg tttccaacaa agcctgagcc
    30601 atcgtcttcc ccagactttt ttatcgataa acaaacagga gctgagacaa aaatcttgtc
    30661 tcagctcctt tttccgaata aaaacggcgt tctcctgcca actccgcggt aataagtcca
    30721 gccaaacgta acatatgaag agctattttt tatttgactc ttcttattac ttgatgcaga
    30781 acagcttttt tacaacctct gataaacggt gttcaaaaac cagtttatcg gtatgaagtc
    30841 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttcata ctcaaaacta
    30901 atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct tttaatgctg
    30961 cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag gaaatagcta
    31021 actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct tcttgaggga
    31081 atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata atttctaact
    31141 ttttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt agattgccgg
    31201 ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca gcttctgcat
    31261 attcacgttt caatgcccaa cctgtttttc catttcctgc agcataaacg aattgaccag
    31321 tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta ttccgtaaaa
    31381 atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga tctggcgcta
    31441 atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa gataccccag
    31501 ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct ggaacgccag
    31561 agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct aaatcatcat
    31621 aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt tcttcactac
    31681 gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc agcgcataac
    31741 caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact ccctattctt
    31801 tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca taaagagtgt
    31861 ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa
    31921 tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac ccgatcactt
    31981 tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg taaaatcatg
    32041 agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca gccttccctt
    32101 gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga aggtaataca
    32161 cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa
    32221 gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga aatggctaag
    32281 atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa aagaccagaa
    32341 aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca tttttctgat
    32401 cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt catgcccctt
    32461 tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata aacggtgttc
    32521 aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc tatttcccat
    32581 ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt
    32641 tattgactac cggtatccag atttctccca tatatttccc gttggtaact cccatttcga
    32701 ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa
    32761 aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgattacaa
    32821 caagatacgt tccttttggg aatgggatga ttcgggtagt gtttggaaca tcctgactcg
    32881 tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat
    32941 aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct
    33001 caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca
    33061 caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc
    33121 ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta
    33181 cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc
    33241 aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag
    33301 cttcgactaa ctcataatct gaagaaggaa gccattctgc atagattttc ccccaagtat
    33361 cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta
    33421 tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt
    33481 gcatcaaact gcccttttct tccaagcggg ctccatcaaa tccataagaa acgttgatta
    33541 cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg
    33601 tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg
    33661 ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc
    33721 ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctgctga
    33781 tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt
    33841 catatccata tctaaacgcc gtttctgtca ctcttgatcc atctaggaga tcgcgattag
    33901 ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa
    33961 agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa
    34021 gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat
    34081 ttatcttcct ccttgcttgc ctttttatca tattatcaaa gaaagtcaat tacttgatga
    34141 atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa
    34201 gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa
    34261 ataaattttg gggaagacac attctaagta aacaaagcgg taaaaatatc ttttattccc
    34321 ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag
    34381 aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta
    34441 tttatttcta acaaaatcac ttcatttttg tcagataaat aaaaaacaaa aaaagttcca
    34501 tctttaaaac acgctattct tttac
//
LOCUS       DASJSZ010000021        34510 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.021, whole genome shotgun sequence.
ACCESSION   DASJSZ010000021 DASJSZ010000000
VERSION     DASJSZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.021"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            212..604
                     /locus_tag="U4B03_000977"
     CDS             212..604
                     /locus_tag="U4B03_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2067848.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="U4B03_000978"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="U4B03_000978"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEN2067849.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="U4B03_000979"
     CDS             3165..4775
                     /locus_tag="U4B03_000979"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEN2067850.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="U4B03_000980"
     CDS             5067..5936
                     /locus_tag="U4B03_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEN2067851.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="U4B03_000981"
     CDS             6101..7372
                     /locus_tag="U4B03_000981"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN2067852.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="U4B03_000982"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="U4B03_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEN2067853.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="U4B03_000983"
     CDS             8763..9026
                     /locus_tag="U4B03_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEN2067854.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="U4B03_000984"
     CDS             9471..9920
                     /locus_tag="U4B03_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2067855.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="U4B03_000985"
     CDS             9977..11395
                     /locus_tag="U4B03_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2067856.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="U4B03_000986"
     CDS             11395..12255
                     /locus_tag="U4B03_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEN2067857.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="U4B03_000987"
     CDS             12429..13202
                     /locus_tag="U4B03_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2067858.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="U4B03_000988"
     CDS             13349..13714
                     /locus_tag="U4B03_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEN2067859.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="U4B03_000989"
     CDS             complement(13862..15070)
                     /locus_tag="U4B03_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2067860.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="U4B03_000990"
     CDS             complement(15089..15367)
                     /locus_tag="U4B03_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067861.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="U4B03_000991"
     CDS             15521..16216
                     /locus_tag="U4B03_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2067862.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="U4B03_000992"
     CDS             complement(16658..17455)
                     /locus_tag="U4B03_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEN2067863.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="U4B03_000993"
     CDS             complement(17452..18636)
                     /locus_tag="U4B03_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN2067864.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="U4B03_000994"
     CDS             complement(18661..19908)
                     /locus_tag="U4B03_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN2067865.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="U4B03_000995"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="U4B03_000995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEN2067866.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="U4B03_000996"
     CDS             21888..22202
                     /locus_tag="U4B03_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEN2067867.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="U4B03_000997"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="U4B03_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEN2067868.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="U4B03_000998"
     CDS             22887..23531
                     /locus_tag="U4B03_000998"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEN2067869.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="U4B03_000999"
     CDS             23546..23875
                     /locus_tag="U4B03_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEN2067870.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="U4B03_001000"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="U4B03_001000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEN2067871.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="U4B03_001001"
     CDS             24863..25687
                     /locus_tag="U4B03_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEN2067872.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="U4B03_001002"
     CDS             25680..26027
                     /locus_tag="U4B03_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEN2067873.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="U4B03_001003"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="U4B03_001003"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEN2067874.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="U4B03_001004"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="U4B03_001004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEN2067875.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="U4B03_001005"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="U4B03_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEN2067876.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="U4B03_001006"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="U4B03_001006"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEN2067877.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="U4B03_001007"
     CDS             31657..32451
                     /locus_tag="U4B03_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2067878.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="U4B03_001008"
     CDS             32441..34483
                     /locus_tag="U4B03_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2067879.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11095 a         6119 c         7248 g        10048 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgattt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat
//
LOCUS       DASJSZ010000022        34474 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.022, whole genome shotgun sequence.
ACCESSION   DASJSZ010000022 DASJSZ010000000
VERSION     DASJSZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.022"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(74..283)
                     /locus_tag="U4B03_001009"
     CDS             complement(74..283)
                     /locus_tag="U4B03_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067880.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(294..1622)
                     /locus_tag="U4B03_001010"
     CDS             complement(294..1622)
                     /locus_tag="U4B03_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067881.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            1758..1913
                     /locus_tag="U4B03_001011"
     CDS             1758..1913
                     /locus_tag="U4B03_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067882.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(1987..2574)
                     /locus_tag="U4B03_001012"
     CDS             complement(1987..2574)
                     /locus_tag="U4B03_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEN2067883.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(2657..3907)
                     /gene="clpX"
                     /locus_tag="U4B03_001013"
     CDS             complement(2657..3907)
                     /gene="clpX"
                     /locus_tag="U4B03_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEN2067884.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(4154..5443)
                     /locus_tag="U4B03_001014"
     CDS             complement(4154..5443)
                     /locus_tag="U4B03_001014"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEN2067885.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(5597..6529)
                     /locus_tag="U4B03_001015"
     CDS             complement(5597..6529)
                     /locus_tag="U4B03_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067886.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(6686..7345)
                     /locus_tag="U4B03_001016"
     CDS             complement(6686..7345)
                     /locus_tag="U4B03_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN2067887.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(7348..8373)
                     /gene="sppA"
                     /locus_tag="U4B03_001017"
     CDS             complement(7348..8373)
                     /gene="sppA"
                     /locus_tag="U4B03_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN2067888.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(8386..8676)
                     /locus_tag="U4B03_001018"
     CDS             complement(8386..8676)
                     /locus_tag="U4B03_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067889.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            9086..10891
                     /gene="glmS"
                     /locus_tag="U4B03_001019"
     CDS             9086..10891
                     /gene="glmS"
                     /locus_tag="U4B03_001019"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEN2067890.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(10963..12318)
                     /locus_tag="U4B03_001020"
     CDS             complement(10963..12318)
                     /locus_tag="U4B03_001020"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEN2067891.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12342..13511)
                     /locus_tag="U4B03_001021"
     CDS             complement(12342..13511)
                     /locus_tag="U4B03_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067892.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(13508..14395)
                     /locus_tag="U4B03_001022"
     CDS             complement(13508..14395)
                     /locus_tag="U4B03_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEN2067893.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(14584..18264)
                     /gene="nifJ"
                     /locus_tag="U4B03_001023"
     CDS             complement(14584..18264)
                     /gene="nifJ"
                     /locus_tag="U4B03_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEN2067894.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            18462..18983
                     /locus_tag="U4B03_001024"
     CDS             18462..18983
                     /locus_tag="U4B03_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN2067895.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(19682..20247)
                     /locus_tag="U4B03_001025"
                     /pseudo
     CDS             complement(19682..20247)
                     /locus_tag="U4B03_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20426..21082)
                     /locus_tag="U4B03_001026"
     CDS             complement(20426..21082)
                     /locus_tag="U4B03_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067896.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(21650..22585)
                     /gene="whiA"
                     /locus_tag="U4B03_001027"
     CDS             complement(21650..22585)
                     /gene="whiA"
                     /locus_tag="U4B03_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEN2067897.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(22619..23617)
                     /locus_tag="U4B03_001028"
     CDS             complement(22619..23617)
                     /locus_tag="U4B03_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEN2067898.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(23614..24498)
                     /gene="rapZ"
                     /locus_tag="U4B03_001029"
     CDS             complement(23614..24498)
                     /gene="rapZ"
                     /locus_tag="U4B03_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEN2067899.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(24633..25364)
                     /locus_tag="U4B03_001030"
     CDS             complement(24633..25364)
                     /locus_tag="U4B03_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEN2067900.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(25368..26633)
                     /locus_tag="U4B03_001031"
     CDS             complement(25368..26633)
                     /locus_tag="U4B03_001031"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEN2067901.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(26896..29712)
                     /gene="uvrA"
                     /locus_tag="U4B03_001032"
     CDS             complement(26896..29712)
                     /gene="uvrA"
                     /locus_tag="U4B03_001032"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEN2067902.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(29722..31716)
                     /gene="uvrB"
                     /locus_tag="U4B03_001033"
     CDS             complement(29722..31716)
                     /gene="uvrB"
                     /locus_tag="U4B03_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEN2067903.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            31975..33477
                     /locus_tag="U4B03_001034"
     CDS             31975..33477
                     /locus_tag="U4B03_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN2067904.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            33479..34216
                     /locus_tag="U4B03_001035"
     CDS             33479..34216
                     /locus_tag="U4B03_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2067905.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT         9814 a         7447 c         5954 g        11259 t
ORIGIN      
        1 aatcagtcaa aaaaaaataa aaaaccagtc aaaaagactg gttttttatt tttttgtcta
       61 tcatcaagcc gttttatcgt tttttatttt cgcttcttgt ttttttcata taaacagaag
      121 caggttttct ttccttatga agaagataat aataaagtcc catcccgcca aacatgacca
      181 aaaaaccgat gactgatcga ataggaaaat taggaacaaa aaagagacag acggataata
      241 atgatgccgg aaccattatt cgataataga gttgtttatc cattttcttc acctcatatt
      301 ttttgtaaag taatcaattg ttctaattct cggtaatcta aaaattgcac acgcagatgc
      361 tgctcacctt tcttgacatc gaattggata cttcgccgct ttttctttat tcgtttgaag
      421 gtaacatcag agagtctatg tttgatttcc tttaacggta attctgacag ggattctgaa
      481 ggaacataga ttcttaagga taaaagatac tcttgatatt tttcatctgt tacctctact
      541 tctagatatt ccctaatata ataacaataa gcttcgattc cattatccaa agcaacagtc
      601 ggatcgataa ctttatagca catcatgcgc gagcgattct cgtcaaccca aggaaaatac
      661 gcctgagcat ttagtgttcc gcgaacacgc ctttctttat aatctccttg ttgaatatca
      721 taactttttg ttacaaaaaa tgaagcattc tttttaggaa caggttgcat ttgtgcattc
      781 tgatactgga cagattggat cgtatggtca gtgtaaatgt attcaacgaa tggcggtttc
      841 aataaaccga caccattgtc ccaagcaaga tgatcaaacc caagctggtc cctaaaatag
      901 gattgaaaca tttgattgtt tggcttttgc cagttaaagg ggtctcgata aagctggata
      961 catatgactt cgttttctaa ttccttcact ttttctactt gttggatgtc gagaaaaatc
     1021 ggtttaggga tgatttgatg ataatagcga ctataataca tttcccaaca cgaagtgaaa
     1081 cataaacctt ggtaaaaaag atcgtatcct ggaaattggt tgcaatcgac tactacttcc
     1141 ccgttttgtt gtttcatttt aggtaaatgt tcgatttcat cctttttctc aaacaatagc
     1201 cgttgagaga tctgttttgt attgtgatag ataaactcgc tataagctcg catataggcg
     1261 aacaccccat gagcaatcat ttttttctga atgaagtctc gaatcaaaaa ttgcttttct
     1321 ttgaaaacag agatagacaa tacatttttt atcaataatt gattatcttc caagcgaaaa
     1381 ctacattcgt tctcgtcatt tcctacaact aaccaacttt cgtctcttac cgatgctagc
     1441 attatttttt cttgaagctt tcgaagatca tacggatccg cttgttgtgt gaaagagcgg
     1501 ataaatgttg gctggatcaa cagacgtgtc aaaatagccc aatcgcttat ttcctgtttt
     1561 ttttcttctt catttaagtc tgtgagctgg atagtcatca ataatttatc ttcgccaatc
     1621 attttctcac ctctttaaaa tctttttata atttaattat aaccaacgat aaagaaaaac
     1681 taaaacttta tgtcttagaa accaatttgg acgattacat gatagactaa gtaaaaagaa
     1741 acaaagaggt gtttacaatg gggttgaaat ttgaacgtac agataattta gataaacttt
     1801 tcgaatcatt tgctgtcgat ccggaaaaaa cagttgcacc aaaagaagag atcgatcgat
     1861 ttctaaaacc acagccacaa aatgaaaaga aaaaagacaa gaaacaaaag taagttaagc
     1921 tcaaaaaaag acttaccagc caaactttta tgaaagagtt tgactggtaa gtctttttta
     1981 ttctttttat gattcaattg cttgttcgat tgctgcccac gcttcttctt ttccctgttt
     2041 tgtaacggaa gaaaagagaa taaacgtgtc attgcgatca aaatccaacg cttttttgat
     2101 ttcgctctca tgcttgttcc atttaccacg agggatctta tctgccttag tggcaacaac
     2161 aattacaggg atatcataat acttcaaaaa ttgatacatc tggatatctt cttgagaagg
     2221 cttatgacgc aaatctacta aagaaacgac cgcacgtagc tggtctctag tcgttaaata
     2281 tgtctcgatc atctttcccc attttgctcg ctcagttttg gatacttttg catacccata
     2341 tcccggaaca tcgacaaaat gtaatgcgtt ctcgatcaaa taaaaattca atgtctgggt
     2401 ctttcctggc ttaccggaag tacgggctaa attctttcga tcaatcaatg tgttgataaa
     2461 agaagatttc cctacatttg atcgtcctgc cagagctatt tctggcaatt gtgtatctgg
     2521 atattgtttt ggtgccactg cacttatgat aatttcagct tgatggactt tcatggtctc
     2581 tctccttaac aaggtgatga ataaagcaag cggctgtcgc aaaagccaaa atgactttta
     2641 cggcagcctc ttttcatcaa cccgcttttt tctctgaatc tttatagatt actgttggct
     2701 caccagacaa ttcagctgct tcttttgtga taatcacttt ttcgatgttt tcatttgaag
     2761 ggatatcaaa catcacatcc atcatgattt cttcaatgat ggaacgaagt cctcgagcac
     2821 cggtattgcg ttcgattgct tttttcgcaa tggctctcaa cgcttcaggt tcgaattcaa
     2881 gttcggtatt atctagagaa agaagttttt gatattgttt gactagcgca tttttcggct
     2941 cagtcaagat ccgtaccaga tcatctgttg tcaatttctc taaagctgcc ataacaggta
     3001 atcgtccgat aaactcaggg atcaatccga attttagtaa atcttcagga atgatatgct
     3061 gcatgacact ttcgtcttca gaaagttttt ggttttgtgt accaaatcca atcgtctttt
     3121 cgcccatacg attcttcaca atcgtttcga tgccatcaaa tgcaccacct acgatgaaca
     3181 atatgttcgt cgtatcaatc tgaataaatt cttgatgtgg atgtttacgt cctccctgag
     3241 gtggcacgct tgcaacagtt ccttctagaa ttttcagcaa ggcttgttgg acaccttcgc
     3301 cggaaacgtc acgagtaata gatacatttt cgcttttacg cgcaatttta tcgatctcat
     3361 caatatagat gatccctttt tctgcacgtt ccacattgta atcagcagat tgaagcaatt
     3421 taagcaaaat gttttctaca tcttccccca cataaccagc ttctgtcaaa cttgtcgcat
     3481 ctgcgattgc aaaaggtaca ttcaatgttt ttgctaatgt ttgtgctaaa aatgttttac
     3541 cagaccctgt aggtccgatc aagcagatat tgcttttttg cagttcaaca tcatctgtgt
     3601 tttcttgtgc ttgctgattg acacgtttat agtgattgta tacggcaaca gctaacgagc
     3661 gttttgcgcg atcttgtcca atcacgtagt tattcaatac ttcaagaatt tctagtggtt
     3721 tagggacttc agtgaattcg cgtacagctt cttcataaaa ttcttcatca atgatttctt
     3781 tacataggtc gatacattca ttacagatgt agacccctgg accagctacg atctttttca
     3841 cttcttcttg ggttttcccg caaaaagaac agcgtacggt ctcattgcta cttggattgt
     3901 catacatggt cttcacccct taacttgcaa aaagtatgca agaaactatc aaagacttta
     3961 cagtctttta ctatcataac atagtttagt aaaaagaaaa agtgtttaac aaaggatcaa
     4021 ctgacctata cctttgcatt ttttactctt aacaaaaaat aaggaactgt gacaaaatcg
     4081 tgtcacagtc ccttatttcg aataaacaat tttctagaag tagcttcttc agaaatgatt
     4141 tattacttga tttctattct ttcgcttctg tcgctgtacc tgtgatcaat tcgattgctt
     4201 ttttcattgt cacatcatgt tccagcatat cttctgtcaa cacgcgtttg atttgttcga
     4261 ttggcatgtt gtatgtttct gataattctt ggatttctgc attgatttct tcttctgttg
     4321 cttgaagatt ttcagcagct gcaattgctt cgatcacaag atttgttctt gtgcgcattt
     4381 ctgcttcacc ttcgaattgt ttatgcaagt cttcttctgt agaaccagtt aattgatagt
     4441 acatttcagg agagatacct tgacgttgca tgttgttcaa gaattcgtcc attgaacgat
     4501 gtacttcatc atgaaccatc acgtgtggca attcaacgat ttctgcgttt tcaacagcca
     4561 tacggatcgc tgcttcatct tttgcatcgt ccgctgcttt ttcttttgtt tctactaatt
     4621 ctttgcgata tttttctttc aattcgtcta atgttgatac ttcgtcatca acatcttttg
     4681 cgaattcgtc atctaattca ggcacttctt ttgcttttac ttcatggatt gtcactttga
     4741 agactgcttc ttttccagca agatcttctg cttgataatc ttctgggaaa gttacattta
     4801 catctagact ttcacctgat ttatgaccaa ctaattgatc ttcaaaacct ggaatgaatg
     4861 agcctgaacc taattcaagt gagtaatttt cgccttttcc gccatcaaac gcttcgccat
     4921 ctacaaagcc ttcgaagtcg attactactg tatcgccatt ttcagctgca gcatcttctt
     4981 tgataactaa ttcagcttgt gcttcttgtt cacgtttgat acgtgcatcc acgtcagcat
     5041 ctgttacttc acggtcttgt ttttcaacaa ctagatcttt atattgtcct aattttactt
     5101 ctggtttcac tgttacttca gcagtgatta cccagtcttg acctttttcc atgctttcaa
     5161 catcgatttt tggttgagaa acaggatcga tgccagcttc tttgacagca gcttcataag
     5221 cttctggtaa tacagcattc aatgcatctt catataaagc ttcttcaccg tacatacggt
     5281 tgaatacttg acgagaaact ttccctttac ggaatccagg tacgttcaag ctttttttca
     5341 ctttattaaa cgcagttgtt aatccttttt ggatttcgtc ttgcgcaatc gtaaatttta
     5401 aaacaccatc attcgtgcct tttttttcaa atgttgcagt catttaattc cctccgagtt
     5461 tagaaattca ttttatacat caaaaacgtt tcatgcatac ctttaaatag tacactaacc
     5521 ctttcaaatt gtaaacatat tgaatggata gaatccttgt ttttcataac atttttatct
     5581 ttttcactga taaaatctaa tggaagaaga tcatttgctg caaaattttt tctctttttt
     5641 ttaaaaaatc ttgtttttca tcttctgtac cttcatacat cgtttttcct gtacacgcct
     5701 ctacagtcag ttgaaaccag cgcttcgggt cttcgatgta tgtatcatag acaggataaa
     5761 caattgcaga ttgcaacgtg aattcttcat ttaacacgga actaagcagc gcatcttcat
     5821 taccgtaacg atctgaaaat acacataatt gctggtaact tgcctgctgc tgcggctttg
     5881 gcagatcttt tggtatgact ttgactaact tttcttcaat cgtcaaatag aaaacaggat
     5941 ggcattcgtt caattgccta agtgtctcca gtaattttgc ccttgctaaa atatggacct
     6001 ctggcgatac aataagttta ctcgctgttt ggataaactc atgataagga agctgttcga
     6061 tctcatcaat agacgcaagc tgacgtgcag gttccaacga aggaagttcc tccgatttat
     6121 tgtggagtgc cttgatcttc tgcctctcca tttgtccgga taattcttct gcaaattgga
     6181 ttttttcaat gacccgctgc tcgaaagctt cataaaaatc tgtacttgct atcaattttc
     6241 tagcatataa aaaatcttgt gtactgatta acaagtcaaa ataaaattca gcaaactcct
     6301 cattttgaag atactgtttt tcatgcaaga gtgcttgctt caatgcttct tttttctcgt
     6361 ccaactcgta aagacaaaaa acaagcaggc tattagcctc aaagctttct ttcaactcgt
     6421 aagctcgttg aaataattct tttgcctgct gatattgctg gtttgctagt gcttgttgtg
     6481 ctactcgcat taaattctca tatttttctg gaaaatcaat cggcttcacg cagtgtcctc
     6541 ctcttgctca tgttcagctc atgttcaata gtagcaaaac aaaaaaagca tgacaattaa
     6601 aaataactga agcaccctct aaaagctaga ttttagatct gacttttaga aggtgctttc
     6661 aactgttact gctttttatt gctcatcagc tgttgacttc ctggttgaat gctttgatca
     6721 gattgatcgt aacaacactt gtattagtga aaaaatcgcc tagatgttgc tttttctgat
     6781 taaagattgc tggaagatac cctaacatca gcaaaggaaa ttttagaatg aaccggcaag
     6841 caccttctct aactaaaacg gttgaccagc taagttcttc ttcatcaaag ctgatgactc
     6901 gaataccaaa gatcattttc ccaatcgttt gaccatgatt gaatttcgtc aataaaataa
     6961 aatacgctag ataaataagt aaggctgtga atccgtagat actcagataa gaatctgaaa
     7021 aattaagcca acctagattt ttggcaatac caagtgttaa acgcgtaata gctccgatac
     7081 acaaaagatc gaccaagaaa gcaaacattc ggataaaaaa acctgcatag aaatacttag
     7141 gaaaatcatt tgcttttggc ggttcatttc ttgtgtccgt atactttttc catttacgtt
     7201 gtttcttttt gatttcttcg ggcgtcaaag gctctctttc aaaagagctc agtacctttt
     7261 gggaaaagtt ggagtcaggt cctacagcag gctgctgttg cttatctttt tcttgttcct
     7321 tattttcatt tgaaagctct gacatgctta ctcacctccg taataataca ttgcttttgg
     7381 tgattccgct gtccctagat tctcaagaac ggataagatc tgactagtct cagaaggctt
     7441 caatccctgg aattctgcta gtttacttcc taaccatgta ctagcaaacc cagatggcgt
     7501 ggaagaatat tctactagtt ccgcatcttc aagctgttgt tcctttcgca tagcttttaa
     7561 agcatcttca ggaaatccca attcatcgac taaacctact tcttttgctt ggacaccatc
     7621 ataaatcctt ccatcagcaa tttttttgac ttcttcttca gatttattcc gtccctcgct
     7681 gacgattgct acaaaacgac tatatgcgct gtcgatatat gcttgaagca ccgcttcttc
     7741 ttcttttgtc tctggtcgcg ttcctgaacc catatctttc aacgctccgc ttttaactgt
     7801 tgtatcttcc acacccagtt tttctagaag tcctgaataa ttcaatcctg acatgatcac
     7861 accaattgag ccggtcactg tttcttctgt tgcaaagatt ttatctgctt gagcagaaat
     7921 atagtagcca ccgctagctg ccatgttttt catgctgaca tacattggga tatcatgttc
     7981 tttacgaatc gtatccaatt tcttagcgat ttccgcactt tcgtagacac cgccaccagg
     8041 agaatttact tctaaaaaga tacctttgac tgttggatct tcttcaatcg ccttcagttg
     8101 ttccataaaa tttttatgat tgtaaccttc agaagcaaat aaacctgaag accctcccgc
     8161 agcaatcgtt ccatctactg ataattttac gattttttgc gtactgtctc cctcttccaa
     8221 cacatttgaa gctagttctt catccccata taaccaactg ttcaatccct caatttgatc
     8281 gttttcctga ggcttagtca aactagctga aactaacgaa accacaaata atcctgcagc
     8341 aatcagaact gctgcccaac gtctcttatt cattctttct cctttctaat acaagttttt
     8401 tatgacgatc acagtttctg actgctcaga agtgtcgaac cgctctgctt tttcttcccg
     8461 cgcatacccg gaaaacgact cttttgtcaa ttcatcttct accgtaaact cacgagcttc
     8521 agcaacttcc aaggcatcag gtgcgatttc ttggatgcta tcccagccag tatctaaaaa
     8581 tattttcgta tcatctgata caccagctga tgcttcgaat ttggacaatt gtttttttaa
     8641 cattcccagt gtcatgatct caatttttga atccattttt actcctcctc tatccgatta
     8701 ctttctattg taccgaactc ctgaaaaaga tgatagataa aaacccatta ttttttgaca
     8761 taaaaagagc atttgacaaa gcatttgata tataccaatt tattttttat tctttttttg
     8821 ttgacaagaa cattcgcaaa aggttatgat tttaaatgtt tccagataaa aaacaaaaga
     8881 ggaggatagt tatacctttt aggatagcgc caggtctgtg taaacagatg acgaggagag
     8941 ggtttatcga aagattcggc ggatggccct agggactgca ctctacagaa aatccacaaa
     9001 aaatatctgc aaagataaaa caaaaggatt ttcctgcagc tggaagcatt catacgaatt
     9061 cattatagaa agtaggatgt tttttatgtg cggaattgtt ggaatgattg gattaaaaaa
     9121 tgtaacacca ggattgattg acggtctaga aaaattagaa taccgtggtt atgattcagc
     9181 tggtatcttt gtctcagatg gcacaactga ttatctggtc aaagcacaag gcagaattca
     9241 aaatttgaaa aacaaaataa atattgatac tacaggaaat ataggtattg gtcatacacg
     9301 ctgggcaact cacgggcaac cttctgaaga aaacgcccat ccacatactt cccaaagtgg
     9361 acgcttcgtg ctcgttcaca atggcgtaat cgaaaacttt gaagaactta aaattgctta
     9421 tttagcaaat gaccacttta ttggtgagac cgatactgag attatcgctc atctaattga
     9481 aactttcgcg aaggatacta caacgcaaga ggcgttttta aaagctttgc gggtcatcaa
     9541 aggttcttac gctttcgctt tgattgatcg gacagcacct gatgttattt acgtcgctaa
     9601 aaataaaagt ccactattaa tcggactcgg cgatggattc aatgtgattg ccagcgatgc
     9661 aatggctatg cttgctcata cgaaggaatt cgttgagatt gaagatgaag agatggttac
     9721 cgtcacatca gaaaaagtta ttattcaaaa ttttgcaggt gacatcgtag aacgttcttc
     9781 attcgaagca caagtagatt cttcagatat cgagaaaggt acctatcctt tttatatgct
     9841 gaaagaaatc gacgaacagc ctattatcat gcgtcgtatc gctcaaaaat acgtaacaga
     9901 agacaatcgt gtctctcttg atcaaaagct ggtcgataca ttgtctgata gcgatcgaat
     9961 ctatatcgtt gcttgcggaa ctagttacca tgcggggtta gcaggtaaac agactctgga
    10021 aaaattgaca cagatccctg ttgaggttca tttagccagt gaatttggct ataacacacc
    10081 tcttctttca aaaaaaccat tctttatttt cttgagtcaa agtggcgaaa cagcagatag
    10141 ccgccaagtt ctggtaaaaa tcaaccgtct cggctaccct tcacttacga ttacaaatgt
    10201 tgctggttct actctttcgc gggaagcatc ctttacactg ctattgcatg ctggacccga
    10261 aatcgcagta gcatcaacga aagcttatac tgcacaaata gctgtactaa ctttattagc
    10321 caaagctatc ggtgataaaa aagaatttga aacttctctt gtattcgata tcactcatga
    10381 attgagttta gttgctaatg cgatggaatc ggtcatcgcc caaaaagatt atttagaaga
    10441 gttggcagaa gtatatctca gcgatacaag aaatgccttt tacattggac gtggcgctga
    10501 ctacaacgtt tctcttgaag ctgctttgaa attgaaagaa atcagttata tccaggcaga
    10561 aggttttgcc gctggcgaat tgaaacatgg aacgattgct ttgatcgaag aaggaacacc
    10621 agtgattgga atcatcagtg aagaagtgac tggtgcgcat actcgaggaa acctgaaaga
    10681 agtcgaaagc cgtggcgcta aaacactagt tattgtctca gagggattag aaaaggaaag
    10741 tgaccagctg gtgctaccaa ctgtccaccc ttatctaact actctaacca cagtcgtacc
    10801 gactcaatta cttgcatatt acgcaacgct attacgggga ttcgacgttg acaaaccacg
    10861 taatttagcg aaatcagtga cagtagaata ataaaagaac aaggaccagt gtcagacatc
    10921 tgacactggt ccttgttctt ttatttttta tgaaaatatc tcttaatcca gtccgatttc
    10981 ttctttgact actgccgcga ttttatcgac atagtaatta actttctcat cagtaggcgc
    11041 ttctgccatc actcgaagta atggttctgt accggaagga cgaactagga ttcttccttc
    11101 tgatcccatt tcagcttctg cctcttcaat cacttttttg attgccggaa catccatcgc
    11161 accgttttta tcacttactc ggatattcac taatttttgc ggatagattg ttacctctgc
    11221 tgcaagctct gataatttct tacctgtctg tttcatgata ttcagtaact gaattcctga
    11281 aagcatccca tctccagttg tgttaaaatc taagaaaatc atgtgcccag actgctcgcc
    11341 gccaaagtta taatcgtttt tgcgcatttc ttctacaacg taacgatcac caacttgtgt
    11401 aacgacatct ttcaaaccga ttccttccac cgctttgtgg aatcctaagt tactcatgac
    11461 agtcgtaacg atcgtatctt ttttcaatcg atttttttct gccaagtatc tagcacagat
    11521 aaacatgatt ttatcaccgt cgacaatgtt tccaagttca tccaccgcaa taatacgatc
    11581 gccatcgcca tcgaaagcta gtccagcatc tgctcctttt tcaacgacca tctcagctaa
    11641 acgttctgga tgggtagagc ctacgccatc attgatattt aacccgtctg gatttgtgcc
    11701 catggtataa aaatctgttt caagatcggc aaacaaacga ttaacacttg ttgaagttgc
    11761 tccgtttgct gcatcgacac aaatggtcaa accggaaagg tcaccgttga tcgtttgtac
    11821 aaggaattga gaatatttca ataagccttc tggaaattct tctacagttc ccaatccttc
    11881 agctgatgga cgaggcaatt catccacttc agcatctaat aatgcttcaa tttctagctc
    11941 ttggtcatct actagtttaa aaccatctgc accgaaaaat ttgattccat tatcttgtgc
    12001 tggattatga gaagcagaaa tcatgacccc tgcgcttgct ttttgcaaac gtgtcagata
    12061 agcaacacct ggtgtagaga tcacacctag ctggaacact tcgataccca ccgataaaag
    12121 tcctgaaatc agcgcttctt ctaacatttg acctgagatt cttgtatccc gccctactaa
    12181 cacgcgtggg cgttttcctt cttcttcatg ctggcttagg acataacctc cgcaccgtcc
    12241 cagcttaaat gcaagttctg gcgtaagttc cttgttggct tcaccgcgta cgccgtctgt
    12301 tccaaaatat ttacccatag ttgtaaaatt actcctttaa cttaattttc tgaggaattt
    12361 gttctgctca tttgtgttga ttcgcttgtt tctgatgttt cttctgtact tgtggtcgag
    12421 ctgcttgtcc cagattccga actggaagaa atggttgttg tcgatggttc cgttcctgaa
    12481 tcagcagatg aaatacttct actcgtactt gtattttctt gtgtccgact gctcgtcgaa
    12541 gcagagctac tgcttgacgt ttgcaacacg ggtgtaatct ccacactaac tgattcgggc
    12601 ttgatactta acccctcttc tactggtatt tcaattttac gagttgttcg atgattgata
    12661 cctgagacat tgatcggtac agcaatactg tcaccgatcg atgcaagcaa atcgctagag
    12721 ccgcttactt ctgcttccag ttttgaaaaa ctgaaatcat agctttctac cccggcagct
    12781 ggtgttcctt gctgtatccc ataaagtcgt accgacttgc ttggcttgat cgaatcagct
    12841 ctcactgtta cttgcactgg atcagagacg atacttaata cttcccctgc tgcattcagc
    12901 gcctgtattg ggacctcact attaatccca tcatctgtga ccatcgaagc gtccacatca
    12961 gctactactc gtttgatttc tgccaatgtt ttgtcacctg tagtgataga gactttttta
    13021 ggactaacgg aagtatcatc aagttcatat ccagatggtg tgaacgttga aggaatcaac
    13081 gtttccacat cgaaatcttt tattaccttt ttctcaaccg ttactgtaat agtcgatggt
    13141 tcgatctttg ctgtgacagc actgctcaaa ttttgaattt ttagcggaac ctcatgtgtt
    13201 ccttcgccca gcttagtgat atctgctgtg acgcggaaca tccgtgtatc ttcgtttgcc
    13261 tctgcattca actgaactcg gtttgcactg gaaagtttga cagtcactgc attttcatat
    13321 ccatggatat agtagcgctt tgaatcataa gaaatcttga ttggcacccc agtaactgtt
    13381 tgattatacg cttcatttcc agaaagcgta tttggaacgg actggccatt tgcattgaag
    13441 tacagcgcaa tactgaacag caatgcaata aaactgtaaa gaatgcttgt ccgtttctct
    13501 tttgagatca tttgcgatcc cctcccttag tcataccgtc taggaaagat tgaatcacgt
    13561 ttttcttgtt ttttgttttc tcttcagcaa tcagttctct acgcaaaatt gccaaatatt
    13621 cttgctggga taagccagcc atcagttcat tatctaatgt aatgctgaca tccccagttt
    13681 cttcagagac aacgatggta atcgcgtcgc tgacttcgct tataccgaca gctgcccggt
    13741 gtcgagtccc gaattctttt ggaatcaaca tactttctga tagaggaaga taggcgcttg
    13801 ctactgcgat ctttccttct tttacaataa ctgcaccgtc atgcagcggt gtattaggaa
    13861 taaagatatt gatcagcagc tctccagtga tatctgcatc taatgcaatt cctgtctcaa
    13921 tatactcatc taatccagta tgccgttcga tagtaattag cgcaccgatt tttcgcttgg
    13981 acatatattg gatggctttg tcaaaagaca agatcatctt ctcatcttcc tgctgttcgc
    14041 tttttgattg tttgaaaagc gaactgcgtc caagatgttc cagtcctctc cggatttccg
    14101 gctgaaaaat cacgatagca gctatcaccc cgtacgtaat gacttggttc atcagccaag
    14161 acaaggtgtg cagaccaatg atttccgcta cgattcggat aacaataaaa atagctacac
    14221 ctttcagaag ctgaatcgct tttgtgcctc gcagcagttg gatcagtttg tatacaagat
    14281 accagacaac cagaatatca agtatgttca ccaaaaaatt cagagagaac aagtcaccgg
    14341 aaaacatttg ctgccaatag tccaaattaa aaagttcccc gatttgaaat gacataaaaa
    14401 gcctctcttc tcttttgaga tttcctctct agtatatcat atgttttaat tttagaggct
    14461 gatctgctcg tatttttctt gagaaaaacg cataattttc aggaaaattg caagtatgcg
    14521 ttttattttt ctctgcaata aatattttta aacttcagag aattattcat tatatttttt
    14581 ctgttatttt tccttttttt cttttgtagc ttgttccatt tttttacgtc gttctgctct
    14641 ggcagctctt ctttcttctg cttcaaggtc cgtcgttttt tcttttctag tcttaacggg
    14701 tacatcgata gattcctctg gttcaagtcg tttcttgatt ttttctaatt ctcccgtcag
    14761 tgcagcatag ttgtaaaaac gtgttttagc gtcttggata gtcttttcaa ataaagcttt
    14821 tgcaagattt ggatttgcgt tctctagcga ggaaaagcga acttgtttcc tcatgaaatc
    14881 aggcatagca gagaaatcag gttttttgta gtccaaagtc attgggttct tgtttttttc
    14941 gaccattgca gggttatatc gatataatga ccaatagcca gaagaaacag cttcttttgc
    15001 ttctttaagc gattctttca ttccaccaag caaaccatgt gtgatacaag gcgtataggc
    15061 aatgattaaa gaaggtccag gatacttttc tgcttcttca aaagccttta tcgtctgcat
    15121 ttgattggca cctgaagcaa tttgcgccac ataaacattt ccatatgtca tcgccatcat
    15181 tcctagatct ttctttgaag catactttcc actagccgaa aatttagcga ttgccgaagc
    15241 tggtgttgct tttgaggttt gaccgccagt attggaatag acttcgttat ccaaaacaag
    15301 aatattcacg tctgctccgc tggcaagtac atgatcgatc ccgccaaaac caatgtcgta
    15361 agcccatccg tctccaccaa tcatccattg gctgtttttt acgaatagat cctttctctt
    15421 gtatatatcc tccagttcag ggacaattga tcgttcgctt tcgatcgcag cttgcagttt
    15481 tgctgctctt tgctgcgttc cttcgctctc aaacatgtgt accaaccagt cttccatcaa
    15541 ttctcgtagt tcttctgata cttttggcaa tacttctttg attgaacttg ccaaagcctc
    15601 gcgacgagtc tgtgccgcta gaagcatacc ataaccaaat tcagcattat cttccaataa
    15661 agaatttgac catgcaggcc cttgaccaca ttcattggtc gtataaggag aagcaggaga
    15721 agcaccgccc caaatagaag aacagcctgt cgcattcgcg atcatcattc gatcaccgaa
    15781 catttgtgtc aataatttta cataaggtgt ttcgccacag cctgagcaag cgcccgagaa
    15841 ttcaagaagc ggttgattga attgtgtaga caagacagta ttctttcctt tgactggatt
    15901 tgctttttgt cttaatgtca ttgcaaaagc ccaattcaca gcttgttctt tctgttcttc
    15961 ataaggcttc atcactaaag ctttttcttt tgcgggacaa gcctctacac acagaccaca
    16021 acctgtacag tcttccactg aaacttggat acggtatttt acgccgtctg cgcctcgcat
    16081 ttctctcacg ataaatcctt ctggcgcttc ttccatttcc tcttcatctg ctaaaaatgg
    16141 acggatggca gcatgaggac aaacaaaggc acattcatta cacatcgtac agcgatcact
    16201 aatccactca ggtacttcta aagcgacgcc ccgcttttcc atagaagccg ttcctaaagg
    16261 catttcgcca gttgtcattc catttgctac aagtgtacca acagagagtt gatttccttc
    16321 ctgtgcattc actggttcaa gtatttcttg tatataacca gtgttccttt tattgatttt
    16381 ttcaggcatc tccaacgttc tccaatggtc tgggatcgcg acttgatgaa gcagttcggt
    16441 cgtccgatcg attgcttgta tgtttttttc tacgatagcc attgatttat gtccatagct
    16501 tttcagggct tcttctttta gcagcggaag cacctcatca aaaggaatga tgtcagctaa
    16561 tttaaaaaaa gccgtttcca ttgccgtatt gatccggcgt cccaaaccga cttcttgggc
    16621 caatctcatg gcattgatcg tatagaatct aatgttattt tcagccaagt aacgttttaa
    16681 ttttaagggc agatgcgttt ctaattgttc gtcgctccaa agagtattca ataaaaaaat
    16741 gccacctggc ttcaatcctt tgaccaagtc atagctatgc aggtaagctg gtgtgtgaca
    16801 ggcaacaagg tccgcatgct cgacaagata cgctgaacgt atcggcgtat ctccaaaacg
    16861 aagatgagag acagtcagac cgccagattt tttcgaatca taataaaaat agccttgcgc
    16921 atatttatcg gtgtgatctc caataatctt gatagctgat ttatttgctc caactgttcc
    16981 gtcagatcca aatccccaaa acttggcctg gaatgtttgc ggttctgtca aatccagact
    17041 ttcctttggt tccaaagatt gataagtcac atcatccacg atccctatgg taaaccgttt
    17101 tttgcgtatg cttgggtctt ttttcaattc atcaaaaact gctgagattt ggtcgggagt
    17161 cacatcttta gaccccaatc cgtagcgacc accaataaca gccgggcgaa gttcagaatc
    17221 atataacgca ctttgaacgt ctaataagag aggttctcca cctgcccctg gttctttact
    17281 tcgatcaaga acagcaattg tttttactgt atgaggcaac ttttgcacga atgtttccac
    17341 tggaaaagga cgataaagat ggatattcaa aaaacctgtt ttccgtccgt ttgcattcaa
    17401 gtaatcaatc gtttgttcaa tcgtctgtgc aactgatccc atagaaacaa tgacttcttc
    17461 cgcatcgtct gctccgtaat aagtcaccaa gtcataagaa gttcctcgta atcggttgat
    17521 ctctttcata tatttttgaa cgatttcagg taatttatca taataggggt tgatcgtttc
    17581 ccgttgttga aaatggatgt ctggattctg gttcgtacca ctaacagtcg gatgattggg
    17641 attcatccct cgtttacgaa atgaagctaa tttctcctga ttgatcagag gagctaattc
    17701 ttcatattcc aaaacttcga ttttttgaat ctcgtgactc gtacggaatc catcaaagaa
    17761 attcaaaaaa ggtacttctc cttcaataga agcaagatgg gcaacaggtg ctaaatccat
    17821 cacttcttgt acactgcttt cagctaacat agcaaatcct gtttgtcttg cagccatcac
    17881 gtctccgtga tctccgaaaa tattcaacgc atttgtcgtc actgcccgac tagcaacatg
    17941 gaaaacactt ggcagtagtt ccccagcaat cttgtacata ttcggtatca tcaaaagcag
    18001 cccttgagaa gcggtatatg tagttgtcag tgcaccagtt ttcaatgatc catggaccac
    18061 accagctgct cctgcttcgg attgcatttc tactactttt actggttgac cgaatacatt
    18121 ttcttttcct gctgctgacc attgatctac taattcagcc atggtcgaac tgggtgttat
    18181 tggatatatt gcagcaatct cggtaaatgc ataagaaata tatgcggctg ccgtattgcc
    18241 atccattgtt ttcatttttc gcatgattca tcatccttgt tcatattctc tcttattatc
    18301 tgttccatta taacgtaaaa cattccattt tttcttttat ttataaaaat atttcacata
    18361 tgtctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    18421 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    18481 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    18541 aaatggtcga attcatcaac ctatcttgtc cgtattgccg acaatggttt gaagaatctt
    18601 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    18661 ataaagaaaa agaaagcttg cagcgaggaa acgtgatgca ccggtatctc acaatcagcg
    18721 atggtcaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    18781 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    18841 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    18901 ttccgactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    18961 aagaattgat ccaaacaaaa taaaaaaata aggagcgtag gacaaaagta aagaacactt
    19021 ttgtctcacg ctctaaacac ctataaacgg cggaagcaga agcaactcct tcttatttct
    19081 tggagttaaa cgcttctgtc ccagcctcct tattccgaat aaatggtgtc caaaagcttg
    19141 catctgccag attatctcat ttcttatgac aagctgagca aattatctcc accaaataat
    19201 ttattcatat ttttgacttg cgcatactaa ctaattctta aattttcata caaaatttct
    19261 caaattccct taacgagtaa tatttatgta cggtgattca cactagtatc ccaatcactt
    19321 tttaagttat cccgatttca attctacttc aaaaatagtt gcttttatgt atgctgttag
    19381 ttgattagat ggtcgtttgt tgagctaggt cgtttatttt aaacaagttg tttctttagc
    19441 ttatacctat ctcgtctacc aaacgcaaga tggcaaacac aaatgcatac gcatctgtat
    19501 ttgccacttt gattcgtata aagctaacaa ctcctaactc ctctcattta atagttgctc
    19561 tcttttttat tcagatacat ttttgctgat cacagcctag tatttcaatg gttttgaaca
    19621 ctaaaccaaa ttgttccatt acaacattca ctggctgatg aaatggatta atagatttcg
    19681 attattcaaa gcgattttct attggtggat tgtgttccca actaacaaga cggattccaa
    19741 agtcattata taactcttca agagttaacc ctttcctgtt cccaatagat gtatctctcc
    19801 cacgctcgat ataaaatatg gagtttcgaa tgccaagacc tggcaaatct ttccttgtta
    19861 cagttacact gctactaact tccccatata aaatccagct cctccaaaat gataatatat
    19921 ttttcgacca tcatctaact catgtaacaa taattgttgt gccctcttgt cactcgccaa
    19981 atgcaaacgc cacgtgcgat accatgccat gctacctgca tctgcagaaa atccatcaaa
    20041 taatgcaaca attgtatctt ctatcacaat cctttcccca tctacttcat attctaatct
    20101 aaaatcaaac tcccctcggg taatcgcagg tcttggtggc ttaggaccca atattattct
    20161 acttaaaacc gcattcgttc ccagatgaaa cacagctata acaacaataa tgattaatat
    20221 tttgtttcta tttgataaat ttctcattat tcgcaaatca cctctacatt ctcacatact
    20281 agttcgtaat actcctttaa tatcaaaata tctactgatc gtaataatat tcgctatgaa
    20341 ttcctactac tcgtataaga aaggccaaat catcagcttt tacttgctga tgatttggct
    20401 tgtttctatt tatcgtaatt gttttctaat caccaaaatt gttaacaatc ggttcagata
    20461 tttcccaatt cactagcctt atattatatt gatttaacaa ttcttcttca tttattgtat
    20521 tttgatcaag cggaccaaat tgttctaata attctttgtc atgtaaaata tcccttctag
    20581 taatgcctct ttccctttca atgaaagaag cactaggaaa agtatgatta taaccattca
    20641 acctatctcc catgtaataa ttagcaggtc ctggaaaata aaatatttgc ttattatcac
    20701 taacttctaa cagcaacact aaatcttctc cgctggctaa acgacttgtc catctacgat
    20761 atctccctat gccttcgttc attccaaatc catcaaattc aactattaac gtgtcttcta
    20821 tcacatgtaa ttcttcattc aattcatact caagtctaaa aggaaattct ccatatgtaa
    20881 tctctggcaa tggtggtgct ggagttctcc aaataaatac aataactgtg aatagaaaaa
    20941 atccaacgat cgatataaat gttatgagac ctgctcgaag taaaacccga tataataaaa
    21001 atgctgtatt tcgctttgct atatctagaa aaataaaaag tactgcaatc ataatgatag
    21061 taaaagttgt aaaaaaacgc atttttgccc tccctttata aacatcgatt ctcaaattct
    21121 aaaccaaatc ttggtagttc aagttcaggt ataacgtatt ccaaccatac atctcaatta
    21181 ctatttatga acttatgatt atcactgaat aaatcactcc atccccttgt taaaacttct
    21241 acattaatta cacctggttg ttttcgacta gctaatccaa tgaacttatc aaccatcctt
    21301 atccaccaac cacgagaatc ttgaccagaa gtaagaagag cagttctcaa ggatgatcga
    21361 gcagttcctt ccagtttttt aattaatcag gccagttata gataaatact tgatcttatc
    21421 tatatcagga cttctatatt aagcaaaaat ttatgtacaa aactaaaatc ttcattcagt
    21481 tgtctccccg ctttttatac gattgtatta aaaacaatag aagcctttcc aaattatgtt
    21541 actccagtaa agatggaatt atagtttgac ggaagaaaag caagtataac ttttaaaaaa
    21601 aactgataca ttcgcatctc ctgcttcatg tatcagtttt ttctttggat taaactgctt
    21661 cttcacgcag tttttccgca aattcattta ttttgcgaat tcggtgatta attcccgatt
    21721 ttgatatcgc acctgaggga atcatttccc ctaattcttt caagctgacc tctggatgtt
    21781 gaagccgaag ttctgcgatt tcctgaagtt tttcaggaag ggcatgtaga ccgacctttg
    21841 cttcgatcaa ttcgatgttt tcgatttgtt tagaagccgc atcaatcgtc ttgttcagat
    21901 ttgctgtctc acaattgact agacgattga ccgaatttct catatcccgt acgattcgaa
    21961 catcttcaaa tcggagcatt gagttggttg caccaatcaa tgttaagaaa tcagcaattt
    22021 tttctgctcc ttttaaataa gaaatatagc cattgcgccg ttcaagcgtt cgagcatgta
    22081 gaccgtaata atttaacata tcgcaaatgt cttggttatg ctgttcataa atagaaaata
    22141 tttccaaatg ataacggctc gtttccggat tattgactga acctgaggcc ataaaagcac
    22201 cacgtaagta agaacgcatt ttttgcgaat ttcccataat ctcgtccgaa acgtgtccat
    22261 tgaacatcat accatccatg atatcaagat ccatcaacac atcttttgtg ccttgcttca
    22321 accggacgat atagacattg ttctttttta atttcatttt tttacgaacg agtagctcac
    22381 ttcgtacctg ataatgatct ttcaaaagag aatagatacg tctggcaatt gcagcatttt
    22441 ctgtttggac gttcaaaata aactgctggt tcacaatacc taaagagccg ttcattcgca
    22501 gcaaagcagc taattccgct ttcgcatgtt ctcgatgaac ttccaaacca gttagttctt
    22561 tcttcacatc tgcagcaaat gacatcctat tctcctcctt tctccaacaa taaattgctt
    22621 aatattttga accgaaaact aaacggaaca attcttctac tactttgtct ccatcgtgga
    22681 agacaccacc atctttcaat tccaaaaagt cggtggaaat cactcgacat ccttcatttc
    22741 tcaatccgtt gaagtcgtgt gcgacttgta ctagatactc atcatagatc tctgggtcca
    22801 tataatcttc aggcactttt tccgtattga ccaatactgt atcgataaaa ggctgattca
    22861 aatgcttgtg taaaacacgt atatgatcgg catctgtgaa atgttccgtt tccccttttt
    22921 gggtcatgat attacagata tagacggttt cagcagttgt cttgagcatt gcttcgccga
    22981 tttctggtat cactaaattt ggaaggatac tggtgaataa acttccgggt cccaaaacga
    23041 ccatgtcggc gtccataatt gctgaaacga cttttcgagc cgctttggct tcacttttat
    23101 catttgtgtt gcagacatac acacggtcaa tggttttacg gtcgatcgcg attttagatt
    23161 ctcctactgc agtcgttccg tctttaaaga cagcatgcaa ggtcaaagga gtttctgaag
    23221 aaggataaac atgtccatct acatgcatca tttttgctaa taattgaatc gcctcataag
    23281 tgcttccccg catctcggaa actgcggcaa tgatcagatt accgatcgta tgattggcta
    23341 aaaactgatc ttcctttttg aaacggtatt ggaaaatatc ttcatagaat tgtggcatat
    23401 ccgataacgc cactaagaca tttctcaagt caccaggagg agccatagcg atcgattctc
    23461 tgattgcacc gctgcttccc ccatcatctg caacagtcac tactgctgtg atatctgctc
    23521 cttgatttcg caaacttttc aggataactg gcagaccagt gccaccgccg accactacga
    23581 tctttggttt tcttatccgg taggttttca ttttcacgac cggttcaccg tctctttcct
    23641 tttatccttg tcccgatgtg tcacattgac tttatagttt ttccctaaag catttgccaa
    23701 acgttcagtc aatgccacag aacggtgttg accgcctgta cagccgattg caactgtcaa
    23761 gctgcttttt ccttctttaa tgtatccagg caatacagtt tccagaagat ccaaaaattt
    23821 atggtagaat gattgcgttt catcaaagga catcacataa tcatagactg gctgatcttt
    23881 tccggtcaat ggtcttagtt ccgggatata atgagggttg ggcaaaaaac gcacatccat
    23941 cacaatatcc gaatcgatcg gcaacccgta tttaaaacca aatgatacaa attcgatccg
    24001 aaatccttgg tctccctttg atttgaatga ctcattgatt ttttcacgta attgtcgtgg
    24061 tgtcaatgtc gtcgtatcaa tgatcacgga agcttgggct ttcaagtcgt ctagtatcgc
    24121 ccgttcttta cgtatccctt cagtgatcaa accgtccatc gccattggat gtgctcgtct
    24181 tgtttctttg tagcgagcca ctagttcttc gtctgaagca tctaaaaata agatagaagt
    24241 gtcgatgaag ttcgtatttt cgatttcgat cagcatgttt tggatttctt caaagaagga
    24301 acgagaacgt aaatccactc ctaaagccat ttttgtgact ttgcctgatt ctttgattaa
    24361 ttcccagaat tttggaatca atcgaggcgg catattatcg atacaaaaat agcccatatc
    24421 ttcaaaactt tggatagcta ctgttttccc tgcaccgctc atacctgtta tgatgactaa
    24481 ttgcaaatta tctgtcataa ttacgaactc ctctcacatt gtgcatagta aaattatatc
    24541 attccgggcg tatcattacc agttttcttt ttcttttaca aaaagatttg gaccaaatat
    24601 cttttaggat atttgcccca aatccaattt ttttactttt ccgatgctgt atccaatgcc
    24661 ttgcttcttt cagctagcgc tcgttcaatc gtccgatctg ctaaaataga ttgggcatcg
    24721 atcactggat aatggttatc ttcagcaaat tcatgcatca atgacaattc tgtgcaacct
    24781 aagataactt tttcacagtt taggcgctct accgcttctt ctaagatttc gtaatatagc
    24841 tcttgattca aataatccga ttccttgatc tcatgataga tcagataatt gattttttct
    24901 tgaagaacag tatcaggaat cactgtttcg aatccaagat ttttgacttc gcgttcatag
    24961 attccagctt tcatacttcc ttctgttcct agaatcgcta ctctccctgt cgtatgctga
    25021 cggacaagtt catttgctgc ttctcttggc atatgaagga taggaatatc cgttgccgct
    25081 tgtaattctt caaaaaagta gtgtgcggtg ttacaagtca acacaatgaa gttcggctgc
    25141 aataaatttt gtttctcaat atcatccagc agaaacggca ttggattttc ttctgatcga
    25201 tctaaaatat aagctgtacg atcaggaact gttgcatgat tgaataagac ataattcaaa
    25261 tattcttgat cttttgtagc tttcgttcgg tggttgatca agcgaacaaa actttctgtc
    25321 gccatcgtgc ccattccgcc taaaatactg aaaaaattct ccatgaacta gccttctttc
    25381 acatgaaaat actttttgaa tcttggaata tagttatgga acatatattt catcaacaac
    25441 cagcgcttga ttgaccggtc tttttcataa aacacagtag taccgtaacg attttctttg
    25501 atcatttgct cagctagttt tttgtcttca ttttcccgag catattctaa aaagattttt
    25561 ttgggcacac ccatccataa tcttgcttta tctgattgat tcgttccata ggtcgtttca
    25621 acaaagggtt tgttaaacac tcgatcttct gtgacgaaac gagccagatt caagccgttc
    25681 aatgtcacaa agaaactgct acgaccttgt ctcaaattga tttcaaataa tttatattct
    25741 ccatctctag gatcatactt catatcgaaa ttggcaaagc ctgtatattc gattttttcc
    25801 aaaaatgcct taatggtttg atagattttt tcgttataat ctggcatgat cacaacgtaa
    25861 ttaccaatcg atgctggtgt aggatcttct aacagtggat gaccaagaca catcatccga
    25921 acttgatgat cttcatctac atacgcattt agcacacgca tattactatc atcacccggg
    25981 ataaaatctt gtacaatcat ttcactttta taaccggctt cgtatatccg gcctaaaatt
    26041 aaatcgaact cttctctagt ctctaaaata aaagcttttt ttcttccttc aaattggaca
    26101 gaaagatact ccacactgtt tgcaggtttc aaagcaactg ggaattcaaa aggcaattcc
    26161 tgttccaaat gtccatttac taacatttct tctctgacga tcaatgtttt aggatacgga
    26221 agatcatatt cttcacaaac ttcataaaag cttactttat tgatcagtct ttcaaataaa
    26281 gaataatcaa tatatggaca gataaaatat tctgacagct cttgtttatg ttgggagatc
    26341 aattctgcat atccatcacc acaagcaatc aaaagatatt ttttactctt atcagaatat
    26401 ctttcttttg ccaaacgaag catcgtctcg ataaacacag ggtctttatc aaatcctgga
    26461 ataacttcta cattgacaat tttactgtag cgggtagggg ctaactgatc cgaagcatat
    26521 gcttggcaga ttttgccgta tgcttcatta aaagaacgtg ccataccata aacgttcatg
    26581 tcgcttccta ataatatagg gataaattct tcattttcaa tactgttcat cattatctct
    26641 cacttactca taatctcttg tttttctttt ccaactacac aaggaatatc ataccataaa
    26701 gaaaaaagca atcaaaggaa actcctccga ttgctcaatt cttttttgaa cggattgaac
    26761 ttataggaag tttttgtcta taccaatcag tgtagaaaga actaccatgt aatgattcta
    26821 gaacactgat attaataaaa caagcaaaaa ttcatttttt tatgtttttt taactatctc
    26881 agctatctat cctctttata ccattatttt tttcaaatag tgacctgtat aactttcttt
    26941 tacgttaatg atttcttctg gtgttccagt cgcaaggatc gttcctccac cttctccacc
    27001 ttctggtcct aaatcaatga tataatctgc tgttttgatt acatctagat tgtgttcaat
    27061 cactaaaact gtgttaccag catctactaa tctttgtaat acatgcaaca agcgggcgat
    27121 gtcatcgcta tgaagtcctg tcgttggttc atctagtata tagaaattct ttccattaga
    27181 gattttgtgc aattcactgg caagtttcat ccgttgtgcc tcaccaccgg acaatgtcgt
    27241 tgctggttgc cccatcgtca cataacctaa gccaacatca acaatcgttt gcagtttgcg
    27301 atgaatcttt ggaatgtgct tgaagaattc tacagcatct tctactgtca tttccaaaat
    27361 atcagcaatg ctttttcctt tgtaatgcac ttctaatgtt tcagagttat atcgtttgcc
    27421 atgacatact tcacaaggaa catagacatc aggcaaaaag tgcatttcga tcttaataat
    27481 tccatccccg cgacaagctt cacaacgacc gccttttacg ttgaaactaa aacgcccttt
    27541 cttataaccc cgcatttttg cctcgttcgt ttgagcaaat aacccacgga tatcatcaaa
    27601 tacacttgta taagtcgctg gattacttct cggcgtccgg ccgattgggc tttgatcgat
    27661 atcgatgatc ttttcgatac tttcgtagcc ggaaattgtc ttgaatttac ctggcttagc
    27721 agaattctta tttaattttt gcgctaacga tttctttaag atactattga ccaatgtact
    27781 ctttccagaa ccagacacac ctgtgactgc tacaaattct cctaatggaa attcaacact
    27841 aatatttttc agattgtttt ctgaagcacc agtgatcttg attgctttcc cattaccttt
    27901 acggcgttct tttggtacag ggattgattt tttccctgaa agatatttcc ctgtcaaaga
    27961 attttcatca ttagctactt cttcaggagt gcctgcagcc acgatttgtc cgccttgttc
    28021 tccggcccca ggtccgacgt caatcagata atctgctgcc cacatcgtat cttcatcatg
    28081 ttcgactaca acgagggtgt tgccaagatc acgcattttc tttaatgagc caatcagtcg
    28141 atcgttgtcg cgttgatgca atccgatcga tggttcatct aaaatataca aaactccaga
    28201 aagattcgat ccaatttgtg tagccaatct gattcgctgc gcttctcccc cagataatgt
    28261 tccggaagcg cgactcaaag ttagatagtc tagtcccaca ttttccaaga atgaaagtct
    28321 gtcatttact tcttttaaaa tcggtttggc aattactttc tcttgttcag ataacgttaa
    28381 gtttgagaag aaatctactg ctttttgaat agacaactca tttgtttgcc cgatattcgt
    28441 tccattgatt tgaacagata gagcttgggg attcaatcga taacctttac atgtctgaca
    28501 agttaattct gtcatataaa gacgcatttg ctcacgcgtg aaatcactgt tcgtatcatg
    28561 ataacgacgc tcgatattct tcaatacccc ttcgaaagtc gtttctacat cgcgaacgcc
    28621 accaaaatca ttttcataat ggaaatggaa aagatcatct gtcccattca agatgatatc
    28681 ctgatgctct tccggtaatt cttcgaatgg tgtatctaaa tcgataccga aactttcaca
    28741 agcctgcgct aacatttgag gataatactg agagctgatt ggattccatg gaacgatagc
    28801 accttgacta agtgtcttcg ttctatctgg aatcactaaa tcttgatcga cttctaattt
    28861 gacacctaaa ccatcacagt ctggacaagc tccaaaaggc gcattgaatg agaacagtct
    28921 tggttctagt tcgccaacag taaatccgca ataaggacag gcatagtgtt cgctaaacaa
    28981 catttcttct tcaccgatca cgtcaaccag agcatagccg tctgccaatc gcagagccgc
    29041 ctctaaagaa tcaaatagac gagaacgaat cccttctttc acaatgatcc ggtcgatgac
    29101 gatcgcgata tcatgttttt tgtttttctc cagctcaggc acttcactta aatcatagat
    29161 ctcaccatcg actcgaacgc ggacgtatcc ctctttttgg attcgttcga atattttttt
    29221 atgttgtcct tttttcttta ccacgatcgg tgccaacact tggatctttg tgcgctcagg
    29281 caaagccatt acttgatctg ccatttgatc tactgattga ctggtaatct cgatatgatc
    29341 gttgggacaa atcggatgtc ctacacgtgc aaacaacaag cgtaaatagt cattgatttc
    29401 cgtcacagta cctactgtcg accgaggatt tttacttgtt gttttctgat caatagaaat
    29461 cgccggactc aatccgtcga tgctatctac gtccggctta tccatttgtc ctaaaaactg
    29521 acgagcataa gcagaaaggc tctctacgta tctccgctgc ccttcagcgt acagcgtatc
    29581 aaatgctagg gaactttttc cagaaccgga caaacctgta acgaccacga atttatctcg
    29641 tggaatcgtt acatctatat ttttcaagtt atgggcacgt gcaccgtgaa tcgtaatttt
    29701 atcgttagcc atgttgtcct cctactttga agcttttaat tctaaaatca tatctcgcac
    29761 attagcggct gtttcaaaat ccaatgcttt cgccgcattt ttcatttctt tttctaattt
    29821 catcaataac gtatcttttt cttctttcgt catttcttca tacgagactt gtaccgcttc
    29881 ttttgtatca tcttccgatt ctttcgtaat agaaatcaaa tctcggattt ctttaatgat
    29941 cgtttttgga acgatcccat gttcttcatt gtacttctgc tgaatcgtac ggcgtcttga
    30001 tgtttcatcc atcgctagac gcatagaatc tgttactttg tctgcgtaca tgattacttt
    30061 accttcttca ttccgagcag ctcgtccaat cgtttggact aaggaacgtt cactacgtaa
    30121 aaagccttct ttatccgcat ccaagatagc aacaagggaa acttcaggta cgtctaatcc
    30181 ttcacgaagc aaattgattc caactaacac atcaaattca cctaatcgaa gatctcgaat
    30241 gatttctgtt cgttccagcg ttttgatatc actatgcaaa tatttgactt tgatccctaa
    30301 ttctttgaaa taatctgtga ggtcttctgc catttttttc gtcaatgtcg ttacaaacac
    30361 ccgctggtct ttttcgactc gttcatttat ttcgcctact agatcatcga tttgtcccat
    30421 gattggacga acttcgatca ctgggtcaag cagccctgtc ggacggatga tttgttggat
    30481 aacggtatct gtttgttcat gttcatatgg tcctggcgtc gcagagacat aaatgatctg
    30541 attgacatgt ttttcaaact cttctaaccg aagtggccga ttgtctaaag cagatggcaa
    30601 acggaaaccg taatctacta gcatctgttt tctcgctcgg tcaccattat acattcctct
    30661 gacttgaggc atggttacgt gcgattcgtc cgccacgatc aaaaaatctt caggaaagaa
    30721 atccaatagc gtataaggcg gttcaccttc tttacgtccg tccatatgcc gagaataatt
    30781 ttcaattccc gacgtgtagc ccatttctaa catcatttca atatcgtaat tggttcgctg
    30841 ttctaaacgt tgagcttcca gaagtttgtt ttcatttcgc aagacagtta aacgctgttc
    30901 taattccgct ttgatattcg ctacggcatg ctccatatgt tcatcattcg tcacaaagtg
    30961 agtcgccggg aagattgcca cgtgttccgt ttctcctaaa acttcacctg tcagtgcatt
    31021 gacttctcgg atacgttcga cctcatctcc aaaaaactcc acacgtaagg cacgttcatc
    31081 tcttgaagcc ggaaaaatct ccacgacatc accgcgcact cgaaaacgac cgcgctgaaa
    31141 atctatatca tttctttcga attggatact gactaagtca cgaatcaact gattgcgatc
    31201 tagttctgct ccctgtcgaa tcgaaactac ttgtttttgg tactcaaaag gcgaacctaa
    31261 tccaaaaata catgaaacgg atgcaatgac gatcacatca tttctttcta ataatgaact
    31321 agtagccgaa tgtcgtaatt tgtcgatctc atcatttaca cttgaatctt tttcgatata
    31381 cgtatcgctt gaaggcacat aagcttcagg ctgataataa tcataataag aaacaaaata
    31441 ttcaacagca ttgttaggga aaaattcctt gaattccccg tacagctgac cagccaatgt
    31501 tttgttatgg gcaataatta gtgttggttt attcactttc tcaataagat tggaaatcgt
    31561 atatgttttc cctgttccag tcgcacctaa taagatttgt gcttttttcc cgccgactac
    31621 gccatccact aattggttaa tcgcttcagg ctgatcacca gccggttggt atttcgagac
    31681 aagctcaaag tgtcgggagg tatctctctc aatcattctt cgtcctcctt ttcattcatt
    31741 ccttttcatt ttacgaccaa taaaaagaaa aggaaagcaa aggcattcct tactaaaaaa
    31801 ttataccaca ccgaacatac tttcgccact tttttatctt attatgcatg ttacgtttta
    31861 taacataaaa tgttagaaac tcgcattttt ttcatttttt cgagcttaca gtcttgtctg
    31921 attcagcact tttcgcctat aataataaaa tattggaaat ttaggaggtt gattatgaaa
    31981 agaaagcatt tatttcactc atttgttttc ttaatgacag gacttgtgac acttgcgttt
    32041 ggacatgttg cacaagctga caacactaca acggcagagt caagcacaag cgagacaatg
    32101 caaaaaatag agcccaaaaa agacgtatac gtaattgcca gtgactctgc ttttgctcca
    32161 tttgaatttc aaaacgcaaa gggcgagtac gaaggcatcg atgtaaaatt gatggaaaac
    32221 atcgccaaac tgcaaggatt taacatccga atggactacc gtggtttctc tgctgctttg
    32281 caagctttgg aatcaggaca agcagacgga atgattgccg gaatgactgt aacagatgaa
    32341 cgaaagaaaa gctatgattt ttctgattct tactttgata gcgggatcca attagctgtc
    32401 aaaaaaggag atacttcgat caagtcttat gaagatttga aaggaaaaac agtcggagca
    32461 aaagttggta ccgaaagtgc tgaattttta agcgaccatg aatctgaata tggctattct
    32521 atcaaatatt ttgatgctgc tgatcaattg tacgacgggt taaaagttgg cgcaatcgat
    32581 gcaatgatgg atgattatcc ggtaatcggt tatgcgatca aacaaggaca agaattggaa
    32641 acaccgatca aaaaagaatc tggcggaaaa tatgcatttg ccgttaaaaa aggacaaaat
    32701 cctgagcttc ttaatatgtt caatgaagga ttgaaagaat taaaaagaac aggcgaatac
    32761 gataagatta ttaaagaata tgtagcggat ggttcagaaa cttccagtga gtcttccact
    32821 gctgatgaat caacatttct tggacttatc caaaacaact atcaagcatt gttgaaagga
    32881 ctttggagaa caattctcct aactttgatc tcctttgctt tggctatagt aatcggtgtg
    32941 atttttggat tattcagtgt agcaccaata cgcgctcttc gcacaattgc tagtatttat
    33001 gtcgatatca tccgaggaat tccaatgatg gttcttgcgt tctttatttt ctttgggttg
    33061 cctggtatcg ttggatttac gattcctgac ttccttgctg gtgttatcac cttgactctt
    33121 aacgcaagtg cttatatcgc agagattgtt cgaggcggga tcaacgccgt tcctgtcgga
    33181 caaatggaag cttccagaag tttaggtctt tcttataatc ggacaatgca aaaaatcatt
    33241 ttgccgcaag cgatacgcat catgatccca tcattcgtca atcagttcgt tatctcatta
    33301 aaagacacaa cgatcatttc cgcaatcggt gtagtcgaat tgctgcaaac tggtaagatc
    33361 attgttgcac gtacaacaca aagtacgtat gtttacttga tcattgcgat catgtacttg
    33421 attttgatca cagcattgac aaaattagca aaagttttag aaaagaaggt gaagtaagat
    33481 ggcagaaaaa atcttagttg agcatttagt caaaaaatac ggcgataaca cagtattaaa
    33541 cgacatcaat gtgtccatca acgaaggaga tgtcgtctgt atcattggcc cttcaggttc
    33601 aggaaaaagt acatttttgc gttgcttgaa tcgtttagaa gaaccttctg acggtgaaat
    33661 tattattgac ggcgcccact tgatggataa aagcacagat atcaacttgg tgagacaaca
    33721 tatcggaatg gtattccagc actttaacct attccctcat ctttctattt tggaaaacat
    33781 cattttagca ccgatggacc tgaagaaaga atctagagaa gaagcagaga aaaaagcaat
    33841 caaattgctg cagacagttg gtctagaaga taaaaaagat atgtatcctg aaatgctttc
    33901 cggtggacaa aagcagcgtg tggctattgc tcgtgctcta gcgatgaacc ctgacatcat
    33961 gctttttgat gagcctacat ctgcactcga ccccgaaatg gtcggtgatg tcttgaacgt
    34021 catgaaaaag ttagcagaac aaggtatgac gatggttatc gttacgcacg aaatgggctt
    34081 cgctaaagaa gtggccaatc gtgtcatgtt cattgacgac ggaaactttt tagaagacgg
    34141 aacgcctcaa caaatctttg aaaatccgca aaatgaacgg acaaaagatt tcttgaataa
    34201 agtattaaat atttaataaa aaccaaaaat gcccggtcgc ttcatgacga ccgggcattt
    34261 ttagtttctt gttttttcac ttaactgtac cagccgcatc gcttgttctc ctgcacaaac
    34321 aagcaatcgc tctacctgtt ccatcgcata agaaagtacc atcggttcat tgacgatatc
    34381 aagtacatag tcgataccac cttcagaaac tatgatgatt atcttattct aaagctggct
    34441 tttttgttgg tacaaacatg acaactacaa aaaa
//
LOCUS       DASJSZ010000023        34366 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.023, whole genome shotgun sequence.
ACCESSION   DASJSZ010000023 DASJSZ010000000
VERSION     DASJSZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.023"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            294..983
                     /locus_tag="U4B03_001036"
     CDS             294..983
                     /locus_tag="U4B03_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2067906.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            970..2178
                     /locus_tag="U4B03_001037"
     CDS             970..2178
                     /locus_tag="U4B03_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2067907.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2472..3743
                     /gene="serS"
                     /locus_tag="U4B03_001038"
     CDS             2472..3743
                     /gene="serS"
                     /locus_tag="U4B03_001038"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEN2067908.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4292..5776)
                     /gene="guaB"
                     /locus_tag="U4B03_001039"
     CDS             complement(4292..5776)
                     /gene="guaB"
                     /locus_tag="U4B03_001039"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEN2067909.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6281..6976)
                     /locus_tag="U4B03_001040"
     CDS             complement(6281..6976)
                     /locus_tag="U4B03_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEN2067910.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7066..8166)
                     /gene="ychF"
                     /locus_tag="U4B03_001041"
     CDS             complement(7066..8166)
                     /gene="ychF"
                     /locus_tag="U4B03_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEN2067911.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8175..8366)
                     /locus_tag="U4B03_001042"
     CDS             complement(8175..8366)
                     /locus_tag="U4B03_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEN2067912.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8387..9277)
                     /locus_tag="U4B03_001043"
     CDS             complement(8387..9277)
                     /locus_tag="U4B03_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN2067913.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9264..10031)
                     /locus_tag="U4B03_001044"
     CDS             complement(9264..10031)
                     /locus_tag="U4B03_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2067914.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10043..10756)
                     /gene="rsmG"
                     /locus_tag="U4B03_001045"
     CDS             complement(10043..10756)
                     /gene="rsmG"
                     /locus_tag="U4B03_001045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEN2067915.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10988..12889)
                     /gene="mnmG"
                     /locus_tag="U4B03_001046"
     CDS             complement(10988..12889)
                     /gene="mnmG"
                     /locus_tag="U4B03_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEN2067916.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12906..14303)
                     /gene="mnmE"
                     /locus_tag="U4B03_001047"
     CDS             complement(12906..14303)
                     /gene="mnmE"
                     /locus_tag="U4B03_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEN2067917.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14602..14985
                     /locus_tag="U4B03_001048"
     CDS             14602..14985
                     /locus_tag="U4B03_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067918.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15153..15911)
                     /locus_tag="U4B03_001049"
     CDS             complement(15153..15911)
                     /locus_tag="U4B03_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEN2067919.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15929..16747)
                     /locus_tag="U4B03_001050"
     CDS             complement(15929..16747)
                     /locus_tag="U4B03_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN2067920.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16744..17100)
                     /gene="rnpA"
                     /locus_tag="U4B03_001051"
     CDS             complement(16744..17100)
                     /gene="rnpA"
                     /locus_tag="U4B03_001051"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEN2067921.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17218..17352)
                     /gene="rpmH"
                     /locus_tag="U4B03_001052"
     CDS             complement(17218..17352)
                     /gene="rpmH"
                     /locus_tag="U4B03_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEN2067922.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17875..19209
                     /gene="dnaA"
                     /locus_tag="U4B03_001053"
     CDS             17875..19209
                     /gene="dnaA"
                     /locus_tag="U4B03_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEN2067923.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19407..20537
                     /gene="dnaN"
                     /locus_tag="U4B03_001054"
     CDS             19407..20537
                     /gene="dnaN"
                     /locus_tag="U4B03_001054"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEN2067924.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20766..21008
                     /gene="yaaA"
                     /locus_tag="U4B03_001055"
     CDS             20766..21008
                     /gene="yaaA"
                     /locus_tag="U4B03_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEN2067925.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            20995..22119
                     /gene="recF"
                     /locus_tag="U4B03_001056"
     CDS             20995..22119
                     /gene="recF"
                     /locus_tag="U4B03_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEN2067926.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22116..24062
                     /gene="gyrB"
                     /locus_tag="U4B03_001057"
     CDS             22116..24062
                     /gene="gyrB"
                     /locus_tag="U4B03_001057"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEN2067927.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24082..26553
                     /gene="gyrA"
                     /locus_tag="U4B03_001058"
     CDS             24082..26553
                     /gene="gyrA"
                     /locus_tag="U4B03_001058"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEN2067928.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26789..27088
                     /locus_tag="U4B03_001059"
     CDS             26789..27088
                     /locus_tag="U4B03_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEN2067929.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27138..27650
                     /gene="ssb"
                     /locus_tag="U4B03_001060"
     CDS             27138..27650
                     /gene="ssb"
                     /locus_tag="U4B03_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN2067930.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27676..27912
                     /locus_tag="U4B03_001061"
     CDS             27676..27912
                     /locus_tag="U4B03_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEN2067931.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28055..30028
                     /locus_tag="U4B03_001062"
     CDS             28055..30028
                     /locus_tag="U4B03_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEN2067932.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30034..30486
                     /gene="rplI"
                     /locus_tag="U4B03_001063"
     CDS             30034..30486
                     /gene="rplI"
                     /locus_tag="U4B03_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEN2067933.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30760..32127
                     /gene="dnaB"
                     /locus_tag="U4B03_001064"
     CDS             30760..32127
                     /gene="dnaB"
                     /locus_tag="U4B03_001064"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN2067934.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32907..34199
                     /locus_tag="U4B03_001065"
     CDS             32907..34199
                     /locus_tag="U4B03_001065"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEN2067935.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10491 a         6687 c         6811 g        10377 t
ORIGIN      
        1 aacaaataat aatatatcaa catcacatgt gaaagtcaat aagaaaatgc ttatttatca
       61 acatttctag cttacatgat cattggttag ttctggtcct ttttcgttcg tctttataac
      121 tccgagagac gtaaaatcct tttgttttca gtttaatgtt catgttcttg tcttattcac
      181 accaactaga tgtctcacct tctaaaattt tccttaacaa agtgctgctt ttatctattt
      241 ctgctttttt ttgctagaat aatagtaaca atatgactct ggaggattta tttatgaaaa
      301 ttttgattgc tgacgatgat aaagaaatcg ttgaattatt aagtatctat gttcacaatg
      361 aggggtatga agcagtaaag gcttatgatg ggaaagaagc attatctaaa atcagaacaa
      421 tccctgatat tgaattactt attttagata tcatgatgcc tgagatggat gggatgcaag
      481 tagtgaaaga gctacgaaaa gaatcccaaa taccaatcat tatgctgaca gcaaaaacta
      541 ctgatatgga taagatcaaa ggactggtcg ctggtgcaga tgattatgtg accaagccat
      601 ttaatccgtt agaagtaatg gctagagtaa agtctttatt acggcgtaca aaaatgcaga
      661 ttgccccaga tcagcctgat gtattagaag taaatgcatt aatcatcaat aaagactctc
      721 atgaagttaa aacaatcgat gggaaagaaa tccagttaac agctttggaa ttcggtatcc
      781 tctacttgct tgctagtcat cctaatcgag tatttagcgc ggatgaaatt tttgagcgtg
      841 tttggaaaca agaaagtatt gtctctgcta aaactgtcat ggttcacgtt agtcatttaa
      901 gagataagat tgaagaagca actggtggtg aaaaagtcat tcaaaccgtt tggggagtgg
      961 gctataaaat tgacgcaaga tagtaaacga gcaactgaaa aacgccgtcg gatcaatttg
     1021 acttcaaaag aaatcagcga attgttagcg gaaggaatca tcacgattat ccttttgctt
     1081 ctattaaatg tttctattct ggttgttatc agttcggtga tcaacagttc accttctttg
     1141 acgaatgcga tttgggattc aaagaatatt tttgcagaac gattgaacac tgatttgttc
     1201 tggaacggcc ggaattttat tattcccttt ttctttctat tagatattgg tgttttgtat
     1261 tggcgcttga ttcgaagata tcgccaaatg caattgcggc atatcatcag cgaacttcac
     1321 tacatcgcaa acggaaatta tgatcatcgt atcccttttg aactgagtgg tgacttaagc
     1381 cgagtggtaa caagtatcaa tggattggtg gatagtaccg tagcagcgat tgaagacgaa
     1441 cgtaaaatcg aaaaatcaaa agatgaattg atcacgaatg tcagtcatga tatccgtacc
     1501 ccactcacgt ccatcattgg atacctagga ctgatagaag atggccagta tcactcagaa
     1561 gaagatcttt taaagtatac gcatactgct tatatcaaag caaagcagat gaaatcttta
     1621 gtggacgact tatttgaata cactaaggta cgccagcctg ctgtcccggt gaatttttct
     1681 gcctttgaca tgatccagtt gatcgaacag ttagcagctg attttgagtt ggaagcttct
     1741 aaaaaaaata tccaaatact tgttcaatca aaagtagact cccttatcat ggatggcgat
     1801 accgaaaaac ttgtacgtgt cttcaataat cttttgacga atgcgttgaa atacggaaaa
     1861 ggagcaacga agatcgtaat cgaagtagaa cgtatcggtt cagaagttgt tgcaacagtc
     1921 aaaaataacg gagcaatgat cccacaacag gcaatcgata atctattcga ccgtttttat
     1981 cgtgtagaag aatctcgttc acaagcaact ggaggaactg gacttggttt agcaatcgct
     2041 caaagcattg tggctttgca cggcggatac atttatgcaa aatcagataa gcaatggact
     2101 tctttcatca tgcaccttcc aatcaagaaa aatgaaaaat tacctattga tacacaggaa
     2161 gttattgacg aaagctgaaa tattcgctat tcttattaca attattagaa taaatgaaaa
     2221 acacagaacc cagtacactt tattcttttt cagagagctg atggcaggtg taaatcagca
     2281 aaggataatt tggaagttcc agttcgttta cgtgcccttt cgaaataaga aaggtcggtt
     2341 caacagccgt tatgtgttca acaagtgcag ctgacgctga acttgggtgg taccgcgaat
     2401 ttcagactga ttcgtcccaa gaataatggg atggatcagt ctttttttat tttcaaaaag
     2461 gaggaaatca tatgttagat gtaaaaatga tgcgacaaaa tattgaagaa gtacaagaaa
     2521 aattgatgac tcggggcgta aaaaaagaaa cattagctga attcatggaa ctagatgaaa
     2581 atcgtcgtaa gctactagtc aaaagtgaag aattaaaaaa atatcgaaat gatgtttctg
     2641 ctgagatcgc ccaattgaag cgtgcaaaag aagatgcttc tgctaaaatc gctgaaatga
     2701 aagaagtcgg tggcaacatc aaagcattgg atactgaaat tgctgaagtg gatgaaaaat
     2761 tgaaacaaat tgcaactact ttgccaaacc ttccccatga ttctgtgcca gttggaacag
     2821 atgaagagga caatgtagaa atccgtcgtt ggagcgaacc acgtacattt gcttttgaac
     2881 caaaaccaca ctgggaaatt gcagaaaatc ttgatatttt agattttgaa cgtggcgcaa
     2941 aagtagccgg tagccgtttt gtctattaca aaggattagg tgctcgatta gaacgtgctg
     3001 tctacaactt catgcttgat cagcatattt acgaacacgg ctatactgaa atgatcactc
     3061 cttatatcgt caacagtaaa gcgatgtttg gtacgggaca atttccaaaa ttcaaagaag
     3121 atgttttcca attggagaat actgatctaa cattaatccc aacagctgaa gtaccattaa
     3181 ctaactatta taatgatgaa attttagatg gtgcagattt acctatttat ttcactgcac
     3241 taagccctgc ttttcgttct gaagccggaa gtgctggtcg ggatacacga ggactgattc
     3301 gtcttcacca attcaataaa gtagaaatgg tcaaattttc ggatgtagag cattcttacg
     3361 aagaattgga aaaaatgaca gctgatgcag aagatatctt gaaaaaattg catttgcctt
     3421 atcgtgtgat ggccttatca actggagaca tgggcttttc tgcagctaaa acttatgact
     3481 tagaagtatg gatacctgca caagatactt atcgtgaaat cagttcttgt tctaactgtg
     3541 aagatttcca agcacgccgc gcaatgatcc gttatcgcga taatgatgga aaaatccact
     3601 atactcatac tctgaatggt tcaggacttg cagttggccg tacagtagct gccattttgg
     3661 aaaactacca aaatgaagat ggttctgtca cagttccaga agcgctcgtt ccttatatgg
     3721 ggaatctgaa agttattaaa taaagaggct gtgaaaaagc cgcttagttt cgagtattaa
     3781 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta ataccgagaa
     3841 actggctttt gaacaccatt tatcgagagg ttgtgaaatc agcgaattaa cctgagtatt
     3901 agagaaaaaa atggaaaaat agtccctcat attttaccgt ttgttgaact aataccgagg
     3961 gtcagctgat tgaacaccgt ttagtggaga ggtcgtaagc ctacccccca gctcctagtt
     4021 acaaggacaa attcaaaaaa cagctcttca tattttatga atgttgtact tgtgattcgg
     4081 aggtggcaag ggaacactgt ttattcaaaa agaggctggg acagaagcgt ttaactccaa
     4141 gaaataagaa gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc ttcttatttc
     4201 cgaaggagtt gcttctactt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt
     4261 ctttactttt gtcccacgct ctttaatgtt tttaagattc aactgaatag tttggtgctt
     4321 cttttgtgat ttgtacatca tgagggtgtg attcttttaa tccactgccg ctcatttgga
     4381 taaattgcgc ttcatcacgt aattgctgta gattggctgc cccaacataa cccattcctg
     4441 aacgcaaacc gccgatcatt tggaaaataa tatctgcaac acttccttta taagcaaccc
     4501 gaccttcgat tccttctggt actaatttat ttgcttcatt gacactacct tggaagtaac
     4561 ggtcttttga acctttttcc atcgcgccta atgagcccat acctcggtat gttttgaatc
     4621 gacgtccttg gaagatttcg aattcacctg gtgattcatc tgtccctgcc aacatacttc
     4681 ctagcatcac tgcatgtccg ccggcagcta atgctttgac gatgtcacca gaatatttga
     4741 tccctccgtc tgcaataatc gctttgccat attgacgagc aactgatgct gcatcataaa
     4801 tagctgtgag ctgaggtaca cctacaccgg ctaccacacg tgtcgtacaa atcgatcctg
     4861 gtccgatacc aacttttacg acatctacac ctgcatcata taaagctttt gtcgcttcag
     4921 ccgtcgcaac gtttcctgca atcaatgttg catctgcaaa agttgaacgg atttcttgaa
     4981 tttttcggat cacacctgca ctatgtccat gagctgtatc gatgatgatt gcatctgccc
     5041 ctgcttctaa taaagcattc gcacgctcaa atgtatcgct tgttacacca acggctgctg
     5101 ctacaagcaa tcgaccatgt tcatcttttg ctgcatttgg aaattcgatc actttctcga
     5161 tgtctttgat cgtgatcaat ccgcttaaac gaccttcgtt atctacgatt ggtaattttt
     5221 cgattttatg tttttgcaaa atcttttctg catcttttag agaggtacct actggggcag
     5281 tcactaagtt gtcttttgtc atcacatcag caatcggcat gtgataatct gttacaaaac
     5341 gcatgtcgcg gtttgtaatt atccctacca attttctgtt ttcaagtgtt tcaacgattg
     5401 gcacaccact gatacgatat ttgctcatca attcttctgc gtctgctaca agatgctgtg
     5461 gcgttaagaa gaacggatca atgattacgc cactttcaga acgtttgact tttcttactt
     5521 catccgcttg ctggctgatc gtcatgtttt tatggatcac acctagtccg ccttgtcttg
     5581 ccatagcaat ggccatcttg ctgtcggtaa cagtatccat acttgcactg atgattggaa
     5641 tattcaactt aatattttta gctaattgta cgctcatatc cacttcgttt ggtaacacgt
     5701 gactttctgc tggtatcaat aagacatcat caaaagtaaa tccttttttc gtgaactttg
     5761 tttcccagtt ggacattttt agtaccactc ctctaattta gtttatctat taaaataatt
     5821 gattttttca tttaagtcaa tgatctactt ggaatcaagt tagctttctc atagaatacc
     5881 aatataaagc gtttacatct gtgtgtatta aaagagattg taaaaaagct gtcctgcatc
     5941 aagtaataag aacagccaat caaaaacagc ttctcatgtt tttgaattgg ttgggtttat
     6001 taccgcagat gcaaactttt gaacaccgtt tagtggagag gctgtgaaaa agctgcttag
     6061 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca catttttgca taaattggac
     6121 ttaataccga gaaactggct tttgaacacc gtttatcgta aaaagggact gtgacaagac
     6181 ttttgtcaca gtcccttact attatttaac tatggataaa caaaatgagg ttctttacta
     6241 cccctagata tctagcaaaa tagctgatct ttttgctagt ttatcttctt gggacggata
     6301 aacttccaat aatcccgtat tttttcttca agtagtaacg gattgctaaa gcagttgccc
     6361 cgataatgat aactactact ggatctaata ctggattgat aactggtggc aatagtgttg
     6421 tcgcagaaaa cagtacgacc caaagaagtg tcgttccacc taggataagc aatgtcttac
     6481 ccattcctgg tttcttgctt cgatctgctc cgggatattc atattgatag atgtatttgt
     6541 acatcatata aaagacccag ccaccgattg cagaacctag aacgagtgta gaaaagccat
     6601 aagctggcgt tgttccacgt gaaaacaatc cggtaatcgc aatcatgata cttaatagac
     6661 caagcaacag caacgtatta tctaaccaca tcaataaagg ggtggatgaa ttttttactt
     6721 ctgctggctt attgatgatt gcttccgttc tttcagagac tgtcccaaac aattgacggg
     6781 ctgttcttcc gcctttttgt tctgcaacta aagtaggcag aatgtcatga agggctttta
     6841 ctttttcttc ttcagacaaa ttcgctgctt ctaaagattt tttcaaatca aaaatgtact
     6901 gttgatttct tttggttaat tttgtttcta aactattgtt ttcttttacg attgctcgta
     6961 attgttctgg ttccattatc taatagtcct ccttagccgt tccttttctc aacggtcgtc
     7021 acaaaagaca ttctaccata ttctacagct agtttgatag cccttttaca cattaaagcg
     7081 gaataacatt acgtcgccgt cttgtacgac ataatctttt ccttctaatc gaacacgtcc
     7141 agcttctttt gctgcatgca tgtttccgta atgatccaag tcttcaaaag agacagtttc
     7201 tgcacggata aatccgcgtt cgaaatctga atggatgatt cctgctgctt gtggtgcttt
     7261 gattcctttt ttgaatgtcc aagcacgtac ttcttgttcc ccagcagtaa agtaagtagc
     7321 taagcctaac aaatcataag cagcacggat caactgatcc aaacctgatt cttcgattcc
     7381 taaagcttct aaaaattccg ctttgtcttc ttcatctaat tctgcgattt cttcttctgc
     7441 acgagcactg accacgatca cttctgcatt ttcttctgca gcaaagttgc gcacttcttg
     7501 tacataatga ttcgcgtctg catctgctac gtcatcttct gagacattgg ctacataaag
     7561 aattggtttt gtcgtcaata aaaatagtga tttaacgatt ttttgttctt cttcagtaaa
     7621 ttcgatcgtt cgggcagatt ttccttcttc cagtacaggt ttgattttat ccagtacagc
     7681 tagttctgcc actgcatctt tatctttggt tttagcaact tttgctacac gagtatagcg
     7741 tttgttgata gattctaagt ctgctaacac tagttccaag ttgatcgtat caatatcttc
     7801 tagtggatca acacgtcctt ctacgtgagt aatattatca tcatcaaaac agcgaacaac
     7861 gtgacaaata gcgtctactt ggcggatatg gcttaagaat tgatttccca aaccttctcc
     7921 tttacttgcg ccttttacga ttccagcaat atctgtaaat tcaaaagtcg tcggaacagt
     7981 ttttttcgga tgtacaagtt ctgttaagcg ttgcaaacgc cagtcaggta cttctaccat
     8041 ccctacgttg gggtcgatcg tcgcaaaggg atagtttgct gcttctgctc ctgcttttgt
     8101 gatcgcatta aacaaagttg attttcctac gttgggcaac ccaacaattc cagcagtcaa
     8161 tgccataaat ttattcactc tttctctgca tttttttcaa ttactttttt taatttcttt
     8221 tcaaaatctc gacgggtcat catgacgata tgtccgcaat tcatacatct gattttgata
     8281 tctgcgccca ttcgaatgac ttcccagcga ttcgtctggc aagcgtgagg ttttttcatt
     8341 tcaacgatat cgtgcaagtc gtacataaaa atagcctcct ttttgtttat tcttcatcaa
     8401 attgaatatt caagatatca agaattcgag tcaaatcttt ttgagaaaga tattcaatct
     8461 cgatcttacc tttcccattt ttttcttgga tagctacact tgtaccaaat ttatccatca
     8521 aacgatcttc gctttctcgg atataatatg gtttttcctt tgctagcctt gggatttttt
     8581 tcttttcttt tccatcattt tgatttaact cagaaacaag ctgttccagt tggcggacag
     8641 ttaaattttc agttactgca cgattggcta atttcaagat caatgtttta ttttttaaac
     8701 ctaacagcgt acgggcttgt cccatagata aacgctgatc ttgtaccatt tccttgacaa
     8761 gatccggtaa agaaagcaag cgcaaataat tggcgatata aggtctgctc tttcctaaac
     8821 gactagctac ttctgcttgt gttagtttca ggtttttcat cagcatctca tatgcttctg
     8881 cttcttctaa cggattcaga tcttctcgct gcaagttttc cagcactgct acttgcatca
     8941 tcgcttcttc gtcaaagtct ctaacgatag cagggatcgt ttctttttca gctagtttgg
     9001 acgctcgaaa acgtcgctcc cctgcaataa tctcatatcc ttttactgcg gaacggcgta
     9061 cgatgatggg ttgaaagaca cctgattgtt caatagaatt agctagttcc tgcaaagata
     9121 cttcttcaaa tgttttacgg ggctgatacg gatttggtcg cagctcattc aatggtagct
     9181 ggacaacggt atcatctttt acatccaccg tctctaagtt ttctaaatct tgaaacagtg
     9241 catcgattcc tcggcctaat cctttacttt tcttcacgac ttaccacttc ctttgccaat
     9301 gcttggtaga cttcagctcc tcgagaacgt ggatcgtagt cgatgatcgg ttttccatga
     9361 ctaggtgctt ctgataaacg aatatttctt ggaatgatcg tttcgtaaac tttttcgcgg
     9421 aagtatttac gtacttcttc tacgacttca tttcccagat ttgttcgtgc atcatacatt
     9481 gtcagtaaga ctccctcgat ctctaattct ggattaaagt gtttttggac taaacgaacc
     9541 gtattcagta attggcttaa tccttccaat gcgtaatact cacactgaac aggtatcaaa
     9601 atcgaatcac ttgctgtaaa tgaattgatt gtcagatggc caagagaagg cgggcaatcg
     9661 atcaaaatat aatcatactg actgcttact tctgctaaag atccttttaa tcttgattct
     9721 cttgccatca ttgacgtcaa ttcgatctct gctcctgcta attggagagt cgcaggcaca
     9781 atactcaaat tttcgtgttc agtgatcaaa gttgcctcat caattggcaa ttcattgact
     9841 agaacatcat agatgtctct tgtcacatca ggttttctga tcccgacacc actggtagca
     9901 tttccttgtg catccatatc cactagcaat acttttttgc caaggctagc aagacaagca
     9961 cctagattca ccgtcgtcgt tgttttgccg acgccacctt tttggttcgc aacagatatt
    10021 atttgtgcca tctcttctcc tcctatttga ttggctgctt attaggcagt ccgggctttc
    10081 ttgggtattt ctttggggtt ggtttctttt tttgaataac gatgatatgc cgttgatcag
    10141 agacattcgg caaggtaacc gcctcttctt tgaggaattt accacctaat aaagcaatcg
    10201 cttgttttgc ttctgtcaat tcctcttcac tttttgctgc tttcaatgca taaaaatagc
    10261 cttcttgttt agccaagggc aaacacaatt ccgctaacac attcaaacga gcaaccgcac
    10321 gagcagtgac aaggtcaaat gatgcacgaa agtgaggatt ttgcccaaat gtttctgccc
    10381 ggtcatgata taaggaagca tcgattccta ataatcgggt taactcagat aagaaggtta
    10441 ttcgtttatt cagagaatca acgatcgtta tctgcagttc tggaaaggca atttttaaag
    10501 gtatgctagg aaaccctgcc cccgaaccaa catcgcataa agataaaggt tgcgtaaagt
    10561 ctacttcaaa tgctaatgta agtgaatcgt aaaaatgctt cagatatacg tctttttcat
    10621 ccgtaatagc tgtaaggttc atcttttcgt tccattcgac taatgtccga taataaatag
    10681 cgaattgatt catttgatga tcggtaagct ctatcccttt ttctgccaag gattggcgaa
    10741 attcttctgg tatcataaat attcctttca ttgtgaaaca gcaattgttt cacagattct
    10801 ctccctactt taacgcaaaa tctctagaca tgcaatcctt ccatatggat tcttaaaaat
    10861 ttgaaaagta agaaaagccg atagactttt tctttctagt atgactcata aaatatacac
    10921 agaagagaga actagctttg agcaatttct gcaaaaacta gttctctctt ctatttatta
    10981 ttacgtatta cgctggttca ctgactttcg caatcttccc ttgttcgata tagaccatta
    11041 ggatacttag gtctgcaggg tttacgccac tgattcggct agcttgggca attgtttctg
    11101 gttggatttt ctgcaatttt tgttttgctt ctgttgctaa tccgttgatt gctgcgtagt
    11161 cgatgttttc tggaatcttt ttcgcttcca tgcgttttaa tttttctact ttttccatgg
    11221 cttttttgat gtagccttca tatttgattt ggatttctac ttgttcgatt tcttttcgtg
    11281 tcaattcttc attttctgga ataaaccgta gaagttctgc ataagtaatc tcaggacgtc
    11341 ttaaaaagtc gcttgctagt acaccatctt ttaatggtgc cgcattaatc gttgctaaaa
    11401 agtcttggac ttcttctgtt ggtttgattc gtacattcat caaacgtttg atttcttgtt
    11461 caactgcttc ttttttcttc aaataattct tgtattgatc ttcttttact aagcctacac
    11521 ggtgacccat ttctgttaat cgcaaatctg cattatcatg acgtaaaagc agacggtatt
    11581 cagcacgtga agtcagcaaa cgataaggtt cgttcgttcc ttttgttacc aaatcatcga
    11641 tcatgacacc gatgtaaccg tcacttcgtt tgatcactaa aggttctttt ccctgaactt
    11701 tcaaagctgc attgataccg gcaatcagcc cttgtccagc cgcttcttca taaccgcttg
    11761 taccatttgt ttgtccagct gtataaagat tttcaacgac tttagtttct aatgtcggac
    11821 ggagctggtg tggctctact acgtcatatt cgatagcata tcctgtacgc atcatttcta
    11881 ctttttctaa tccagcaata gaatgaagca tgttgacttg aacatcctct ggtaaagaag
    11941 tagataaacc ttgaacatag acttcttccg tatttaagcc ttctggttcc aaaaataatt
    12001 gatgacgtgg tttgtctgca aaacgcacga tcttatcttc gatggatgga caatagcgag
    12061 cgcctacccc ttcaacgatc cctgtaaaca ttggggcacg atgtaagttg tctcgaataa
    12121 tttggtgagt cgtctcacct gtataggtca gccagcaaga ttcctgatct agtttatagg
    12181 cactatctgg tgtgctgaag ctaaaatgat tcggctcttt gtcaccaggc tgctcttctg
    12241 taacagaata gtcgatcgta cttgatttta ctcgaggcgg tgttcctgtt ttgaaccgtt
    12301 cgatagcaaa gccaagttct tttagatgat tcgctagtcc aatagaaggt tgagagttat
    12361 tcggacctga tgaatatttc aattcaccga taatgatttc tccgcgaagt gctgttccgg
    12421 ctgtaataac aactgctttg gcccggtagc tcgctcctgt ggacgtcact actccatgac
    12481 aaacaccatc ttcgacgatc agttcttcga cgattccttg tcttagcgtc aaatttggtt
    12541 ctttttcgat cgtatgtttc atttctgttg aataagcatg tttgtctgct tgtgctcgca
    12601 atgctcggac agctgggcct tttcctgtgt tcaacatacg catttggata taggttttat
    12661 cgatattctt acccatttct ccgcctaacg catcgatttc tcgaacgaca acgcctttag
    12721 ctggtccgcc tacagaagga ttacatggca tgaatgctac catgtccaaa tttattgtca
    12781 gtaataatgt ttttgatccc atacgtgcag cagcaagtgc agcttctgaa ccggcgtgtc
    12841 ctgcgccaac aacgatcacg tcatacgcat ctgcttcata atgttccaat cgtttgttcc
    12901 tccttttatt ttcctaaaca aaattgactg aataattgag tgattagctc atcttgtaca
    12961 ctgtcgccga caacttctcc tagatagtcc cagcaacgcg tcatatctat ttgaacgaga
    13021 tccacaggca ttcctgaatc aatacctgca atgacttctt caagtgagag ggaagctttt
    13081 tctaacaacg cgatatggcg ggtattagaa acataagtag cgtctctttc tcctgtttct
    13141 ccgctgaaga ataaatcaga aatagctgat tccaaacgat ctagtccgtc gtttttagct
    13201 acggaaattg aaaaaactgg ttcattttcg atcaatttct tcaatttttc ttgttccagc
    13261 tgtgctggca aatctgtttt atttagaaga atgatccgtt tcaatccact tgtcgcttcc
    13321 aataattgtt catcttctgc tgtcagtggt tcgctttggt ttagaacaag taagatcaag
    13381 tctgcttctg ctaatgcttt tcggcttctt tcgaccccga tccgttccac cacatcttct
    13441 gtttcccgaa ttccagctgt atcgattagc ttgagcggta ccccccgaac attgacatac
    13501 tcttcgatta catctcgagt cgttcctgca atatccgtaa cgattgcttt ttcttctctt
    13561 aacaaatggt ttaacaaact tgatttgccg acatttggcc ggccgatgat tgccgtactt
    13621 aacccttccc gcagaacctt cccttgcttg gatgtagcaa gtagcgcttg gattctttgt
    13681 tggatcatct gcgctttttc aagcaataac ttcgtagtca attcttcgac atcatcatat
    13741 tctggataat caatatttac ttctacttgt gctaatgtct ctaaaatctc ttgtctcaat
    13801 gagcggatca aagcagacag attgccgtct aactgattaa gagctagtcc cattgctttg
    13861 tctgtttttg cccgaatcaa atccatcaca gcttctgcct gtgaaaggtc aacacgacca
    13921 ttcaagaatg ctcgttttgt gaattcgcct ggctcagcta atcttgcacc ttctcttaac
    13981 aatagctgca aaatttgatt gactacaacg atcccaccgt gacaattgat ttctactaca
    14041 tcttcccgcg taaatgtctt tggtgctctc atcacggaga ccatgacttc atcgacaagc
    14101 tgattgctct tgggatcaac gatatgtcca taatggatcg tatgacttgg tacttctgat
    14161 aatcgtttgt ttccagattg ataaacttta tctgctagtt gcacagcttg atcgccactt
    14221 aaacgaacga tactgattgc accttctcca ggtggggtag aaattgccgc aatcgtatca
    14281 aattctaaag taatttcagc cattttttca cttccttttt cattttgcgc acaaaaaaag
    14341 tgcccactcc accctttatg cacaataaac ggtgaacttc gcactttgac tgcttgttct
    14401 tgattaaatt ctggtcatag attatcacac ttgctataag gaatcaagtg tttaaaacaa
    14461 ttagatgaag ttgagcctgt tctgcttcca ttaccttata tttaattgtc cttggatgtc
    14521 tctttgcgtt ctttggttta ttcgaagaga agatactgta taaacaaggc tttatctttc
    14581 ttaaaaaaaa cggtacacta aatggtagcg ctatctaaac tatacacttt tgaaaggaga
    14641 aaatatgtga gcccgttcag atctgctgca ttggaagaag aaagtgaccc tcggatcaac
    14701 gatgtcgttt tatctacagg gaatatcaac cagaaatata tcgtccgcga tatcgttttt
    14761 gttgtggaat gcttagaagc tgatttgttt gacccatctt ttcccaaaga tgaccttttc
    14821 ttaacaacga agaggaaatt aaagacgaag gtacttgaat atggtgcgaa tgcagtaatc
    14881 aactgtcatt ttgagtatca gcaaaaaaaa cgcgacggaa agtattttct ggaacttttt
    14941 gcttatggaa cagtgatcca atttatccaa acaacgattg gttaaatgaa acagacaaaa
    15001 aaagcggatc aaaactgaag acagtttcga tccgcttttt tctatcaaag ttagtgacta
    15061 cttcatcgta tactctgata agaatgattt ctagtaaaat atacttggtt tgaaaaactt
    15121 tgtcaacgtg atttattcaa aaagtaatgg aatcaataat attttttagc aggttctact
    15181 actaaataac gataaggttc ctcaccttcc gaatgtgttt ttacatgttc atctttgctt
    15241 aatgcagcat ggatttgttt acgttcaaat gcaggcattg gttctaagaa aacaggctgc
    15301 cctgtccgtt ttactttttc agccgtgcga tcagctaaac gctgaatgat ctcttggcgc
    15361 ttttcacggt aattgcctac attgactact acagacagct tatttcttgc taaacgatgg
    15421 atgaagactt gtgccagata ttgcaatgca ttcagtgttt ttccatgttt cccaatcagt
    15481 atgccttgct tttgtgtttc taaatggaaa acaaccatat tatttgattc tctttccatt
    15541 ttgacaagag ctggagcatc aagctgtttt gaaatttcag taagataaac tgccaattcc
    15601 gttaaagctg cttcatcacc tagctcactt acttcttctg ttttttcttc aagaatcgtt
    15661 tcgttgacta cctcgatctc agctgttggt tcttcttcca aaatttcttc tacagtctgt
    15721 tctactgttt cagaaacttt ttcgctgata cttggctgga tcgaaagccg tgcattcttt
    15781 cgacccattc ctaggaaacc tttttttcct tcttccagta tttcgatttc gacttggtct
    15841 ttggccaaac ccaaatcagc cagacctagt tggacagctt cgtctattgt tgcagcttca
    15901 taaatcggca ttagcattac ctcccctatt atttccgttt tttcttagga ttcattgctt
    15961 tcttaagagc acgttctctt tcgcggattc tccgtgcttc ttcttcacgc tcttttcgaa
    16021 ttttgaaagg gttgttgatc agcagcgttt gtacaacttg gaaggcattg gaaactaccc
    16081 agtataatga aagaccgctg gctaaattga cacccatcac taaaatcatt acaggcatca
    16141 cgaaattcat gatcttcatt gatgcattcg attcaatttg actcatgcta gataaatacg
    16201 tacttgcaaa agtaaagatt gctgctaaaa tcggcaaaat aaatgttgga tccgcgttcc
    16261 ctaagttcaa ccataagaaa tgcccttgtt tcaactctgg tacacgtgaa atagattgcc
    16321 ataacgccat caagattggc atttgtacta ataaaggaag acaaccagca tatggattga
    16381 cgccattttc tgcatataaa cgctgctgct cttctcttaa cttactttgt gtttccggat
    16441 ctcttgaact atattgttgc tgcagtgctt ttaatttcgg ttgtaattct tgcgttttac
    16501 gcatgctctt tgtttggaaa tgcatcaacg gcaataggat cacgcggatg atcagtgtaa
    16561 ataaaatgat ccctatccct gcattaccaa atgacaacgc cttgatcgcc tcagcaaagt
    16621 aataaacaat ataacgatcc caaattcctg tactctgtgc gctgacttcc ccagttccgc
    16681 aaccggataa gacgatgacc aaactaacga taccggccat caaaagtaaa cgtttgtatt
    16741 tcttcactta ctcttttcct ctctgtcgat tatttttgca agttttaaga cgtggactaa
    16801 attggagcga acctcatcat aagtcaaccc cttcacactt ggtctagcaa tcacaataaa
    16861 atccaactcc ggatcaattt cttcttttat agaataaact gctgctcgga tttttcgttt
    16921 gacttcattg cggtttaccg cgttgccgac cttttttcct acagataaac caactcgaaa
    16981 atgaggctgt cccggttttt ccaaacggta aacgacgaat ttgcggttag caaaagaatc
    17041 tcccgtatgg aacacggctt gaaactctcg ttcttttttt actcgatacg cttttctcat
    17101 tagttttctt tccttttcta tctctcactt acgctcataa tcataccata attacgattg
    17161 tcctttcatc atttccctaa aaaagagtat aaaaaaaacc actgaaaccc cagtggccta
    17221 agctgaaaga acttttcttc ctttgcgacg acggctagct aacacacgac gtccattttt
    17281 agtactcata cgtttacgga atccgtgaac tttttgacgt ttacgtttat ttggttggta
    17341 tgttcttttc atatattcca cctccaaaat gttcttatct attttataaa cacagacata
    17401 ctttgatagt ataacgacaa agtagtgatt ttgtcaatat aaaatagaag atcgattgtt
    17461 aaaacttatc cacaagatta tttttttcta tgggaatcct atgtggaaaa agttatgcac
    17521 agggattatc cacagataat aaaaggctgg ttttacacag aaaacacacg tgtaaaaaaa
    17581 gtaatccaca atcaggcgat ttcctgtgta ttccgtctcc taactattgg tatatctgta
    17641 ttttttatcc acagacgcaa gaggaaatct attggttttt acgagttttc cacctgtgca
    17701 ttagtggtgt ggaaaacttt ttttgacaac tgtgtttttc tcttttttct ttttgtcgat
    17761 tgtgtggata attgtaatta aaaaattttt ttcttcggtt tctgtggaaa actattgaaa
    17821 aaaatgctaa attagtacta gttattcact ttttaatgaa ggaggaggcc ttatatggta
    17881 tccctcgatg ctttatggaa tgaattaaaa gcaacatacc aaaaggattt atcgcctgct
    17941 agctataata catggatcga aacagcgaac cctcgaaccc ttgatcaaaa ccagctcgtt
    18001 gttgaggttc ctagcaagat tcataaagaa tattgggaaa aaaacctggc gacaaaaatc
    18061 gttgaagtcg gttatatgtt atcaggaaat gagattatcc ctcgctttat tactggcgaa
    18121 gaagcagagc aagaagaagt tatagaagaa aaaaatccaa aagttgtggc acctagtccg
    18181 ctaaaaaaag ccatgctgaa ccctaaatat acctttgata cgtttgtcat tggtaaagga
    18241 aaccagatgg ctcatgcagc agcgctcgtt gtagcagaag atccaggttc tatttataac
    18301 ccgctcttct tttatggagg cgtgggactt ggtaagactc acttgatgca tgcaattggt
    18361 catcaaatgc tgcaaagtca gcctaatgcc aaagtaaaat atgtaagcag tgaaactttt
    18421 gctaatgatt tcatcaattc gatccaaaac aaaacagcag aagagttccg tcaagaatat
    18481 cgaaatgttg atttactgct agtggatgac attcaatttt ttgcagaaaa agaagctacg
    18541 caagaagagt ttttccatac tttcaatgcc ttgtacaatg aagggaaaca aatcgtacta
    18601 accagtgatc ggttgccaaa cgagattccg aaactgcaag aacgccttgt ttctcgcttt
    18661 gcatgggggc tgtctgttga tattacaccg ccggatcttg aaacaagaac agcgatttta
    18721 cgtaaaaaag cggcggctga acgtctagag atcccagatg acacactaag ctacatcgca
    18781 ggacaaattg attctaacat tcgtgaatta gaaggtgcgc ttgttcgagt gcaagctttt
    18841 gctgcgatga atagtgaaga tatttcgact agtctagctg cagacgcact gaaaacgtta
    18901 aaatcaggaa aaggacatcc tcaattgtca atcctgcaga tccaagaaga agtggcaaag
    18961 tattatcaca tccaattgaa agatctcaaa ggaaagaaac gcgtcaaatc tattgttgtt
    19021 cctagacaaa tcgctatgta tttggcacgt gagctcacag ataattcttt accgaaaatc
    19081 ggagctgaat tcggtggcaa ggatcataca acggttatcc atgctcatga aaaaatccag
    19141 cagctgatgg attctagtgt cagtatgcaa aatgaagtat cagaaatcaa aaacttttta
    19201 ctaaattaat ttgtcgattc tatcaactga aaatagcatg tggataaaag aaaaaaacgt
    19261 tcaaaagtta tacacacttt ttccacagct gttttttcgt ttctctctta cgttttttga
    19321 gttttccaca gaatcaacat gccctattac ttttattatt ctttttaact aataataaat
    19381 aaacaaacat tcaaaggaga tacatcatga aagttacttt aaaccgagct agctttatgc
    19441 aggaattgca aactgttcaa cgagctattt caagcaaaac cacgatccct attttgacag
    19501 gtgtaaaaat cacactgaca caagaaggtt tgactttgac ggggagcaac gctgatatat
    19561 caattgaaac ttttttgtct gttgaaaacg aaaaagcaaa tatgcaaatc gaatctactg
    19621 gttccattgt tttacaagca cgtttcttta gcgaaatcat tcggagactt cctgaagaaa
    19681 catttacttt agaagtttta gaaaataaac aagtagcgat cacttctgga aaagcgaatt
    19741 ttatcgtaaa tggattagat gcagataact atcctcatct tcctgttgtc gaaagccata
    19801 accagatgaa attacctgta cacgtattga ctaaactaat caacgaaaca gtttttgctg
    19861 tctcacaaca tgagagtcgt ccaatcttga caggtgtgca tttcatttta tctgataatt
    19921 ctttattagc tgtagctact gattctcacc gtctaagtca acgcgtgatt ccagtagaac
    19981 aagcggctga tcattttgat attgttattc ctggaaaaag tttgatcgaa ttatctcgct
    20041 cattaacaaa tgaagaagaa atcgtcgaaa tcagcattat ggaaaaccaa gtgttgttca
    20101 aaacagaaac gatgtatttc tattcccgtt tgctagaagg aaactatcca gataccaatc
    20161 gtttgattcc ttcaagtttt aatacagagg ttgaattttc tgttcctagc tttttagcgg
    20221 cgatcgaacg ggcttcttta ctttctcatg aaggccgtaa caacatcgtt cgtttatcta
    20281 ttcgtccaga tgcggttgtt ttatacggaa attcgccaga aatcgggaaa gtagaagaaa
    20341 gcttgagtta tacagcaagc agtggcgatc cattagatat ttctttcaat ccggattata
    20401 tgaaagcagc gttgcgtgca tttggagata tgagcatcaa agtgaaattt atctctgcta
    20461 ttcgtccatt tacattggaa ccaacggaag atggcgttca atttattcag ctgattactc
    20521 ctgtacgtac aaattaacaa tgattttcaa tgttcttgaa aatttaattg aaaagaaata
    20581 gaaaaaataa aattttaaac agaaggccct aaaatctctt ttaaggcttt ctgtttattt
    20641 ttgtttaaat cacctttttt agattaaatc gtcttatatg gaaaaatata tggataaatt
    20701 tgttttattg cgaaaaaaaa ggtataatag agtatacttt taataatgaa aatgagggat
    20761 gggcattgaa acaacaagtc attctaaata cggaattcat gacgttgggc caaatgttga
    20821 aagaagtcac agttatcggt agtggcggac aagcaaaatg gtatttggca gaaaacactg
    20881 tgttagtcga tggcgaacca gaaaatcgtc gtggacgcaa actttatccc ggcatgatgg
    20941 ttgaagttcc agaagtagga acttttttta tggtaaaaaa aggaaacgac gataatgagg
    21001 ctgaataagc tgtatttaaa aaattatcga aattacgaag agctgaacat cgaattttcc
    21061 aagaacctcg tcatcttttt aggagaaaat gcgcaaggaa aaacgaatct tttggaaagt
    21121 atctacgtat tagctatgac gcgaagtcat cggacaagta gtgagcagga attgattggc
    21181 tggactgacg atcaggcgat gattcaagga gagatcacaa aaggttcatc cactcttcca
    21241 ttagaaatct tattatctaa aaaaggccga aaaaccaaga tcaaccatat cgaacagaaa
    21301 aagttaagta cttacgtggg acaaatgaat gtcattttat ttgcaccaga agatctttct
    21361 ttagtaaaag gcagcccgca gatcaggcgg aagtttttag atatggaatt aggccagatc
    21421 aatccaatct atctttatga tcttgtccaa taccaagcaa ttttgaaaca aagaaaccag
    21481 tacttgaagc aattgaatga aaaaaaacaa actgatttgc tttatttgga tattttgtcc
    21541 gaacaactag cagcgtttgg cagcaaagta ttgaaagcaa gagcacagtt cgtcaagcga
    21601 ttggagtttt gggctaattc tttgcatcag caaatcaccc atcaaaaaga gcagctggaa
    21661 atcgagtatc tcaccgcagt cgattcttta gaaacacata cgcaagagca aatccaagaa
    21721 caatttctcg cacttttgaa ccaaaataaa aagaaagacc tttttcgggg aacaacgaca
    21781 gttggtcctc atcgtgacga tctttccttt tttattaatc agaaaaatgt gcagacttat
    21841 ggatcgcaag gacagcaaag aacaactgca ctaagtgtca aattagcaga aatcgatttg
    21901 attaaagaag agacagggga atatccaatc cttttgttag atgatgtgat gagcgagctg
    21961 gatgataatc gtcaactcca tctgctagaa acaatcgaag gaaaagttca aacgttctta
    22021 acaacaacga cacttgacca tgtaaaaaat aaaatgactg ttgaacctga gattttttat
    22081 gttcaacaag ggaaactaaa aggagaagaa actgaatgac agaagaaaga agtttagtag
    22141 aacgcgctaa agagtatgat gccagtcaga ttcaagtact tgaaggactt gaagccgttc
    22201 gtaaacgacc tggcatgtat atcggttcga ctagttctga gggtctgcat caccttgttt
    22261 gggaaattgt cgataattcg atcgatgaag tattagcagg ttttgcaacg aaaatccatg
    22321 tgattattga aaaagacaac agtatcaccg ttattgacga cggtcggggg atacctgtag
    22381 atatccaggc aaaaactgga cgtcctgcag ttgaaacggt ctttactgtt ttacatgctg
    22441 gaggaaaatt cggcggtggt gggtacaaag tttctggtgg acttcacgga gtaggatctt
    22501 ctgttgtaaa cgcgctttct acacagcttg atgtcaaagt atacaaagaa ggaaatgtgt
    22561 actatcaaga atatcgtcgt ggagctgtag tggacgactt aaagattatc gaacaaacgg
    22621 atcgccatgg aacaacggtt cactttacgc cagaccctga aatttttaca gagacaactg
    22681 agtttgattt ttcaaaattg gctacccgga tccgtgaact tgctttcttg aaccgcggga
    22741 tgcgtatatc gattgaagac aaacgagaag aagaaccagt catcaatgaa taccattatg
    22801 acggcgggat caaaagttat gtcgaatact tgaatgcaaa caaaaccgtg atttttccag
    22861 aacctgtata tttggaaggt gaacagcaag atatcgcagt tgaagtctct atgcaatata
    22921 cagacggcta ccattccaat atcatgagtt ttgccaacaa tatccatacg tatgaaggtg
    22981 gaacacatga atcaggattc aagactgctt taactcgtgt gataaatgat tatgcccgaa
    23041 aacaaaagct gatgaaagaa aatgacgaca acttgaccgg tgaagacgtg cgagaaggat
    23101 tgacagctgt tatttctatc aagcatcctg accctcaatt cgaaggacaa acaaaaacaa
    23161 aattgggcaa ttccgaagta cggacagtca ctgatcggtt atttagcgaa catttcatga
    23221 aattcttgct ggaaaaccca agtgtgggcc ggcaaattgt tgaaaaagga ttactagctt
    23281 ccagagctcg tctagcagcg aaacgtgcgc gtgaagtaac gagacgtaaa ggtgctttag
    23341 agattagtaa tctgccagga aaattagcgg actgctctag caacgatcct gaaaaatgcg
    23401 aattgttcat cgtcgaagga gattcggccg gcggatcagc taagcaaggt cgtaatcgag
    23461 agtttcaagc catcttgcct attcgtggga aaatcttgaa tgttgaaaaa gcaagcatgg
    23521 acaagatctt ggcaaatgaa gagatccggt ctttatttac tgctatgggt acagggtttg
    23581 gagaagattt tgatgtatcg aaagctcgct accacaaatt agttatcatg actgatgccg
    23641 atgtcgacgg cgcgcatatc cgtacattgc tgctgactct tttttatcgt tacatgcgtc
    23701 ctattgttga agctggatac gtctatattg ctcagccacc tttatacggg gtaaaacaag
    23761 ggaagaatat tacgtatgta caaccaggaa aaaatgcaga agaagagttg aaacaagtcg
    23821 ttgcttcact tcctgcctca ccaaaaccaa gcgttcagcg ttataaagga cttggagaaa
    23881 tggatgatca tcaactatgg gaaacaacta tggacccaag caatcggatg atggcccgcg
    23941 tcagtgtgga cgatgctatt gcagcagatc agatatttga aatgttgatg ggggatcgag
    24001 tagaaccccg tcgtgcattc atcgaggaaa atgcacacta tgtgaagaac ttagatattt
    24061 aatcatgaag gaggaaaaag aatgagtgaa gaaatcagag aaaacatcca tgatgtcaat
    24121 ctaaccagcg aaatgaaaga ctccttcatc gattatgcga tgagtgttat tgtcgcacgt
    24181 gcacttcctg atgtcagaga tggtctgaaa cccgttcatc gccgtatttt atacggcatg
    24241 aacgaattag gtgtaacacc ggataaagcc cataaaaaat ctgccagaat cgtcggagat
    24301 gtcatgggta aataccatcc tcatggagac attgcgatct atgagtcaat ggtacggatg
    24361 gcacagccat tcagctaccg ttatatgcta gtggatggtc atggaaactt tggttctgtt
    24421 gacggtgacg gtgccgccgc tatgcgttat accgaagcac ggatgagcaa aatcgctaca
    24481 gaaatgctgc gtgatatcaa taagaacact gttgatttcc aaagcaacta tgatgataca
    24541 gaaaaagaac cagttgtact acctgctcgc ttcccgaatc ttttagtgaa cggaacaacg
    24601 gggatcgccg taggtatggc cacgaatatc ccacctcata acttgtctga ggtagtagcg
    24661 gctatcgatt tgctgatgga taatcctgat gtgacgacca atgaattgat ggaagtatta
    24721 cctggaccag attttccaac aggtggtttg gttatgggga aatcaggaat ccgaagagcg
    24781 tatgaaacag gtaagggttc tattaccgta cgtgcaaaag ttgagattac tgagatgcca
    24841 aatggtaaag aacggatcat cgtgacagaa ttgccttata tggtcaataa agcaaaattg
    24901 atcgaacgga tttctgaact gcacagagac aagcgtatcg aagggatcac agatttgcgg
    24961 gacgaatctt cccgtgaagg tatgcggatc gtcattgaca tccgtcgtga cgccagcgca
    25021 tccgttatct tgaataactt atataaattg acatccttgc aaaattcatt tggattcaat
    25081 atgttggcga tcgaaaaagg tgtgccgaaa gttttgagct tgaaacaaat ccttgaaaac
    25141 tatatcgaac atcaacgcga agtcatcaca cgacggacgg tctttgaaaa agaaaaagca
    25201 gaagcaagag cacacatctt agaaggtttg cgtattgctt tggatcatat cgatgaaatc
    25261 attgcgatta tccgcgggtc taaatcagat gacgaggcaa aagcaacaat gatcgaacgg
    25321 ttcgatttat ccgatcgcca gtctcaagct attttagata tgcgtttacg ccgattgacc
    25381 ggtttggaac gggaaaaaat cgaaaatgaa taccaagaac tcttaaaact gattgaagat
    25441 ttaactgaca ttctggcaag accagaacgt gttacagaaa tcatcaagaa tgaattagca
    25501 gacttgaatc aacgatttgg tgataaacga cgcacagaat tactagtcgg tgaggtctta
    25561 agtctggaag atgaagattt aatcgaagaa gaagaaatcg ttattacatt gacaaacaat
    25621 ggttacatca aacgtttggc aaataatgaa ttcagagctc aacgaagggg tggacgtggt
    25681 gtccaaggaa tgggtatcca caatgacgac ttcgtcaaaa atctggtttc ttgttctacc
    25741 catgatacat tgttattctt tacgaataac ggaaaagtgt atcgggcaaa aggatacgaa
    25801 attccagaat acgggcgtac agccaaagga atcccaatca tcaatttact aggtatcgat
    25861 tcgagtgaaa gcatccaggc aatcattgcg gtgacgggag aagcagaaga aggccactat
    25921 ctcttcttta cgacgcgtca aggtacagta aaacgtactt ctgtcactgc ttttgctaac
    25981 atccgaagta atggtctgat cgcaattggt ttgaaagaag acgatgagct cgtcaatgtg
    26041 ctactgacag atggtcaatc taatatcatt attggaacac ataacggcta ctctgtcact
    26101 ttcaaagaag aagcagtccg cgatatggga cgtacagctt ccggggtcag agggatacgc
    26161 ctgcgtgagg aagattatgt cgttggggca gccttaatga aggaaaatca agaagtatta
    26221 gtgatcacag aaaaaggtta cggaaaacgg acaaaagtat cggaatatcc agtgaaaggc
    26281 cgcggaggta aagggatcaa aacggcgaac atcactgaaa aaaatggtcc actagctgga
    26341 ttgaccactg tttctggaga tgaagacatc atgctgatta cagataaagg tgtcattatc
    26401 cgtttcaacg tatcgactgt ttctcaaact ggacgttcaa cattaggcgt acgtttgatg
    26461 aagatggaag ctgatacgaa agttgtcacg atggcagcag tcgaaccaga agctgcagaa
    26521 caaacagttg aagaacaagg atcagaagaa taaccatcaa gactaagtgt gtaagctagc
    26581 aagaataacc gattaaaaaa tctggttatt cctgctggct tttttgatta gttgaatttc
    26641 tcttaaaata ttcgaaactg ttcattctgt attgcttttg accaaaatag agtgtataat
    26701 tagagcttgt gagtagtcta attaattaga taactctcct tgctcttagg aacctaagag
    26761 ccgaaagtcc acagggaggt gaatatcaat ggaaaacacg aaatatgaaa tcatgtacat
    26821 tattcgtcca aacattgatg aagaagcaaa aactgcttta atcgaacgtt tcgatgcaat
    26881 cttgaaagat aacggagcag aagttatcga atcaaaagat tgggaaaaac gccgtttcgc
    26941 ttatgaaatg aacggttacc gtgaaggtat ctatcacatc gttaaagtat cttctccatc
    27001 atcagcaaat gctgtcaatg aatttgaccg tcttgctaaa atcaacgacg atatcattcg
    27061 tcacatgatt gttaaagaag aagcttaatt gttgtttcat gtgaaacaat agaaaacgat
    27121 gagaggagat gactattttg attaataatg ttgtattagt tggacgtctg acgaaagatc
    27181 cagatttgcg ttacacagca agtggtacgg cagtcgcaac atttactttg gctgtgaatc
    27241 gtaattttac aaaccaaagc ggcaatcgcg aggctgattt tatcaactgt gtgatttggc
    27301 gcaaatcagc agaaacttta gcaaactatg ctcgtaaagg aacactttta ggtgttactg
    27361 gacgtatcca aactcgttct tatgataatc agcaaggaca acgtgtatac gtgacagaag
    27421 tagtagctga caacttccaa ttgctggaaa gtcgttctgc atctgaaaat cgccaacaaa
    27481 gcggcggatt ccaaagttct ggtcaatcag ctggcggatt tggcggaaac aataactcta
    27541 atcaaacatc tcaatcatcc aacggtatgc ctgatttcga ccgtgatact tctgacccat
    27601 tcggctcaag ttcaacgatc gacatttcag acgatgattt accattctaa acgaatttga
    27661 taggagggaa atagaatggc acaacaaaga agaggcggac gtaaacgtcg taaagtagac
    27721 tacattgctg ctaaccatat tgaatatatc gattataaag atatcgaatt actaaaaaga
    27781 tttatttcag aacgcggtaa aatcttgcca cgtcgtgtaa ctggtacagg cgctaaaaac
    27841 caacgtaaaa ttactgttgc aatcaaacgc gctcgtatca tgggcttgct acctttcgtt
    27901 agcgaagact aataaatcct aggaattgaa tgactgatcc ttttgatcag ctattcaatt
    27961 cctttttttc tgtaaaaata acctctattc gtttagtcgc tcgaatgacg aaagttatgg
    28021 taaaatcaaa ttagtcatat atgggaggaa aattatggaa aataaaaaca atcatttatc
    28081 cccgaaacct ctcttcctta tcctgtttat attaatcgaa gtattggtag tcttaatttt
    28141 accatttcgt tttgttagcg cttcggtcat tctattgatg aatttatggg gtgttcgcta
    28201 ttggttgaaa atgaaacgtt ggcttatttt aaataaacgt gaaaaaatcc agcaggcttc
    28261 gactgtagct gaaaccaatc tagcttatgt ctcagaagtc atgcctgtag gagtgattgc
    28321 ttatcagcca tcaagccaaa aaatcgaatg gctcaatcca ttagcattga aaatcaaaga
    28381 tcaaagtgga ttaacagagg aagaattgct agatactttt tttactgcaa aagaaaaaaa
    28441 gaaaaaccaa gtctatctag cagataaaac atttcagttt gatatggata cagtaaaggg
    28501 tgttgtttat tttaatgata ttacccaaaa ccagttgtta aaactccatc agacgaacag
    28561 tcagccggtg attggtattt tatctattga taactatgca gatgccattg ataaattaga
    28621 tgacaaagag atttcttatt tgaactcttt cgtcactaca ctagtttctg actggatgaa
    28681 tgaataccat gtctttttca aaagaatcaa cgcagaacgg tttttcttcg tagcacagat
    28741 gatcgatctg aaaaagatga tggataaaaa atttgatttg ctggatcgct ttcgagcaga
    28801 agctgaaaaa cagagctcgc ctatcaccct tagtatgggg attgcttatg gctcaagaac
    28861 cctcgatgaa atcggtggag aagctcagaa taatttagat gttgctttag tccgtggagg
    28921 cgaccaagtt gtcgtgaaag aggtaaaaga aggagcaaaa cctgtttatt atggggggaa
    28981 atctgctaag aatgtcaaac gcacgcgtgt tcgttctcga gcgatgagta ccgctttgcg
    29041 tcggattttt gatgaaacag gcgatgtgtt tatcatgggg cacaagtttc cggatatgga
    29101 tgctttaggc tctgcttttg gtgtcagttg tctggcacgc ttcaaccaga aaaaagctta
    29161 catcgttatc aatgagaacg agatcattcc agatgtagaa cggtgtcttg aagaaatcca
    29221 taagcatcct gaattagaaa tgcaactaat ctctccagaa cgtgccatag agctgaaaaa
    29281 agaagaggac ctcctcgtga tggtggatta ccataaacct tctctatcga tctctcaggg
    29341 cctatatgag caatttgata agattgtgat cattgatcac catcgtcgtg gggaagagtt
    29401 tccaagtaaa cctctgctga cttatattga atcttcagct tcttcagctt cagagctagt
    29461 ttctgagctg atccaatacc aaagcagcaa aacaaatcgc ttagatcgtt ttgaggccac
    29521 attattgctt gcagggatgg ttgtagatac aaatagtttc aatgtccgta caactgcacg
    29581 aacttttgac gttgccagct atttaaggtc ttgtggagca gacgcatcct tagtacaata
    29641 tttattgagc tcggatttag cgacatactt ggagatcagc aatttgattg ctggaagcga
    29701 ctatgtgact tctgatattg ttgtggcggc agggacagac gaaaaagagt ataatagtgt
    29761 tatagcagca aaaacagcag atacattgct ttcgatgatc aatatcaatg cagctttcgt
    29821 cattacaaag cggacagacg gcttgattgg gatcagtgcc agaagtaccg gcacgatcaa
    29881 cgtccaaatc atcatggaat ccttaggtgg cggaggacac ttcacaaacg cagctactca
    29941 actggaaaat acgacagttg aggaaacaaa acagaagcta tttgctgcaa tcaaagaaaa
    30001 tatgaatcaa atatatgata aggagtaatc caaatgaaag taattttttt acaagatgta
    30061 aaaggaaaag gtaaaaaagg ggacatcaaa gaagttccag caggttatgc acaaaattat
    30121 ctaatcaaaa ataacttggc aaaagaagca acgaaaagca gtatcagtca gttagcagga
    30181 caaaagaaag cacaagaaaa acacgaagct gaaatcttgg aagaagctaa aaagttaaaa
    30241 gaattcttgg aaaaagaaga tactgtagtc gaattgaagg cgaaagcagg ggaagacagc
    30301 cgtttgtttg gttcgatccc ttcaaaacaa attgctgaag ggttaaacaa acaatacaaa
    30361 atcaaattag ataaacgaaa aatcgaatta gaaaatccga tccgttcact aggctacaca
    30421 aacgtacctg ttaagcttca tcatgaggtg acggctacga tcaaagttca tgtcgtacaa
    30481 gaataacaaa tcttgatagc gatgttcagc tggtaagtat aaccagaagg aaaaaagaca
    30541 aaccgaaatt tatctgagaa aacagctgca gcaatgaaag caaaagctgc aaatcgattt
    30601 cagattgatt tcggtttctt ttttgggaac agcaaaatag cataaagaaa acgagtataa
    30661 taaaagagaa ctattttttt attacacttt cggtacgaat gatgactttt ctctcatttt
    30721 cttgtaaaat gaagcaattg aagacaagga gcgatacgta tgaatgaagt gtggcaggat
    30781 cgaatcccac cgcaaaatat tgaagctgaa caagcggttt taggctctgt ttttcttgac
    30841 gcagaaacga tcattgatgc catggagtac atagagccaa aagactttta tcgtcgtggc
    30901 catcaaatta tttttgagac aatgatcgag ttaaatgatc gtaatgaagc gattgacgtg
    30961 gttactgtca aagaccggct ggaacaagcc aatttgttag aagatgccgg tggtttaagt
    31021 tatctctcag acttggcatt agcagttccg acagcagcaa atatcgtcta ctatgcgaaa
    31081 atcgttgaag aaaaatcatt gctgcgtaca ttgatccaga cagcaacagg aatcgttacg
    31141 aaaggattcg agcaaggaga agaagttcaa acaattttag atgatgccga acgcagtatc
    31201 ttggaggttt cggaaaaaag aaatcgaagc ggctttttat ctattgctga agtactgaac
    31261 tccacgattg aaaatatcga tcgactagct caaaacaatg aagaaatcac aggtctacca
    31321 actggctatc aaggtctgga taaaatgaca gcaggtttcc agcccgaaga actaatcatt
    31381 cttgctgcac gtccagcggt aggtaaaaca gcgtttgcat tgaatattgc tcaaaatgta
    31441 ggaacaaaaa cagatcgttc tgttgcgata tttagtttgg aaatgggggc agaatctctt
    31501 gtaaatcgga tgctttgtgc agaaggttcg attgaggcca gccatttacg tacaggtcag
    31561 ctttcggaag aagaatggtc gaatctgatc gttgcgatgg ggagtctttc tcgagcaaat
    31621 atttttatcg atgatacacc agggatcaaa atctctgaaa tccgtgcgaa atgtcggaaa
    31681 ctagcgcaag aaaaaggcaa tctaggcttg atattgatcg attatttgca gttgattgaa
    31741 ggtaacggta aagagaaccg tcaacaagaa gtatcagata tctctcgcca actgaaaaaa
    31801 ctggcgaaag agctgaaagt acctgtcatt gctctttcac agttatctcg tggagtagaa
    31861 cagcgacaag acaaacgacc agtgctaagt gatattcgtg aatccggatc aattgaacaa
    31921 gatgcggaca tcgtagcctt cttgtatcga gatgattatt atgaaagaga aggcggagat
    31981 gacgatgatg gaaacgagcc aaaaaatgac aacgtcatcg aagtcatcat cgagaagaac
    32041 cgaagcggtg cacgaggaac agttgaacta ctgtttatca aagaatacaa taagttttcg
    32101 tcgctttcgc caagagaaga attttaaaga agaggttgtg agccttgagc aatcccgaga
    32161 gctctcgtaa gaagatgcaa ggagtaaccg caggaacgac tagagtactt aatttaaaca
    32221 gatctccaaa gggattgtga caaaagtctt gtcacagtcc ctttttccga ataaacggtg
    32281 ttcaatcagc tgacccttgg tattagctca gcaaacgtca aaatatgagg aactattttg
    32341 acgtttgttt ctctaatact caggtcaaaa cgctgatctt acgacctctt ttccttaaac
    32401 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat catcaaacat gagaagcagt
    32461 tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacaacc tctttcttaa
    32521 acggtgttca aaagcttgca tctgcggtaa tacgcccaat caaacgaaaa atatgagaag
    32581 ctatttttgt ttggctgttc ttattacttg atgcaggaca gctttttcac aacctctgat
    32641 tcacggtgtt ccaaaagtag cttcttcgaa aaataagtcg aaatttcaca aaaatttgag
    32701 aagcaatttt cgtacattcc ttcttatttc ttgaagctga ccacttctgt tacaacctct
    32761 tttttaataa ccattctaaa aagataaagt tcgtttttta agcgttttta taaaataaaa
    32821 ataaaaatca ttcgatttta tttgaaatat gtatctatta ttgatacaat ggttcaggat
    32881 taaataatat agaacgaggt gttcacatgt catctgtagt agtagtaggt acgcaatggg
    32941 gcgatgaagg gaaaggaaaa atcacagact ttctcagtga aaatgctgaa gtgattgcac
    33001 ggtaccaagg tggcgacaat gcagggcaca caattaagtt taacggtgtg acttataaac
    33061 tgcatttgat tccttctggg attttttata aagacaagat cagtgtgatc ggaaacggcg
    33121 ttgtagtcaa tcctaaatca ttggttaaag aattagctta tttacatgaa aaaggtgtag
    33181 aaacaagtaa tttgcgtatt tctgaccgcg cgcacgtaat cttgccttat catatcaaat
    33241 tggaccaatt acaagaagat gctaagggcg aaaataagat tggtacaacg attaaaggaa
    33301 tcggacctgc ctatatggat aaagcagctc gtgtaggtat ccgtattgca gacttattag
    33361 ataaagaaat ttttgaagaa cgtcttcgtt tgaatctaga tgacaaaaac cgtcaatttg
    33421 taaaaatgtt tgattcagaa ccacttgatt ttgacgatat ttttgaagaa tattatgaat
    33481 acggacaaca aatcaaacaa tacgtcacag atacttccgt tatcttgaat gatgctttgg
    33541 atgcaggcaa acgcgtgttg tttgaaggcg cacaaggtgt aatgctggat atcgaccagg
    33601 gaacttatcc ttttgttact tcttctaacc cagtagccgg tggggtaaca atcggaagcg
    33661 gtgtagggcc ttcaaaaatc aataaagttg tcggcgtctg caaagcctat acgtcacgtg
    33721 tcggtgatgg accatttcca actgaattat ttgatgaaac aggagaacaa atccgcgaag
    33781 taggacgaga atacggtacg acaactggtc gtccgcgtcg tgtaggctgg tttgattctg
    33841 tagttatgcg ccattctaaa cgagtttcag gtattaccaa cctatcttta aactcgatcg
    33901 atgtattaag tggattggaa acagtaaaaa tttgtacggc ttatgaatta gatggcgaac
    33961 taatctacca ctatccagca agcttgaaag agttgaaccg ctgcaaacca atctatgaag
    34021 aattgccagg ctggtcagaa gacattacag gatgcaaaac acttgcagaa ctgccagaaa
    34081 acgcacgtaa ctatgtacgt cgtatctcag agcttgttgg tgtacgtatc tcaacatttt
    34141 ctgtcggacc tgatagaaca caaacaaaca tcttagaaag cgtctgggcg caaatctaag
    34201 gataaaaacg acgactgaat caggacgttc ttaccagagc gagtcgaagt gtttatctat
    34261 acgataataa taacttgaag aggtagtgaa agagacgttt agccttattg ccgataggct
    34321 gtctcttgaa cactgtttat tcagaataag ggactgtgac aaaaac
//
LOCUS       DASJSZ010000024        34097 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.024, whole genome shotgun sequence.
ACCESSION   DASJSZ010000024 DASJSZ010000000
VERSION     DASJSZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.024"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(29..1261)
                     /locus_tag="U4B03_001066"
     CDS             complement(29..1261)
                     /locus_tag="U4B03_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEN2067936.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="U4B03_001067"
     CDS             1516..2085
                     /locus_tag="U4B03_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2067937.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="U4B03_001068"
     CDS             complement(2263..2703)
                     /locus_tag="U4B03_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN2067938.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="U4B03_001069"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="U4B03_001069"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN2067939.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="U4B03_001070"
     CDS             3657..4580
                     /locus_tag="U4B03_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN2067940.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="U4B03_001071"
     CDS             complement(4805..5044)
                     /locus_tag="U4B03_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2067941.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="U4B03_001072"
     CDS             complement(5049..5468)
                     /locus_tag="U4B03_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2067942.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /locus_tag="U4B03_001073"
     CDS             complement(5695..6933)
                     /locus_tag="U4B03_001073"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2067943.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="U4B03_001074"
     CDS             complement(7040..7675)
                     /locus_tag="U4B03_001074"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2067944.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(8017..8652)
                     /locus_tag="U4B03_001075"
     CDS             complement(8017..8652)
                     /locus_tag="U4B03_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2067945.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="U4B03_001076"
     CDS             9164..10306
                     /locus_tag="U4B03_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN2067946.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="U4B03_001077"
     CDS             10299..10505
                     /locus_tag="U4B03_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN2067947.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="U4B03_001078"
     CDS             10584..11777
                     /locus_tag="U4B03_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2067948.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11982..13121
                     /locus_tag="U4B03_001079"
     CDS             11982..13121
                     /locus_tag="U4B03_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEN2067949.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="U4B03_001080"
     CDS             13114..13914
                     /locus_tag="U4B03_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2067950.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="U4B03_001081"
     CDS             13914..14741
                     /locus_tag="U4B03_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2067951.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14719..15453
                     /locus_tag="U4B03_001082"
     CDS             14719..15453
                     /locus_tag="U4B03_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2067952.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="U4B03_001083"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="U4B03_001083"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN2067953.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="U4B03_001084"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="U4B03_001084"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN2067954.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="U4B03_001085"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="U4B03_001085"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN2067955.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="U4B03_001086"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="U4B03_001086"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEN2067956.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="U4B03_001087"
     CDS             19038..21071
                     /locus_tag="U4B03_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067957.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..22395
                     /locus_tag="U4B03_001088"
     CDS             21115..22395
                     /locus_tag="U4B03_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEN2067958.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22605..23411
                     /locus_tag="U4B03_001089"
     CDS             22605..23411
                     /locus_tag="U4B03_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2067959.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23423..24643
                     /locus_tag="U4B03_001090"
     CDS             23423..24643
                     /locus_tag="U4B03_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2067960.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24633..26219
                     /locus_tag="U4B03_001091"
     CDS             24633..26219
                     /locus_tag="U4B03_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2067961.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26258..28396
                     /locus_tag="U4B03_001092"
     CDS             26258..28396
                     /locus_tag="U4B03_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2067962.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28764..29747)
                     /locus_tag="U4B03_001093"
     CDS             complement(28764..29747)
                     /locus_tag="U4B03_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN2067963.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30125..31516
                     /locus_tag="U4B03_001094"
     CDS             30125..31516
                     /locus_tag="U4B03_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN2067964.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31532..32572
                     /locus_tag="U4B03_001095"
     CDS             31532..32572
                     /locus_tag="U4B03_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2067965.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32591..33676
                     /locus_tag="U4B03_001096"
     CDS             32591..33676
                     /locus_tag="U4B03_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN2067966.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33709..>34097
                     /locus_tag="U4B03_001097"
     CDS             33709..>34097
                     /locus_tag="U4B03_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN2067967.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKH"
BASE COUNT        11060 a         5833 c         6716 g        10488 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt tatatcgtgt atttacagag
    21541 atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg
    21601 atcaatacga atataatcgg aagttctttg atgactcttg ctttattgat cagtggattt
    21661 gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt tgttgcagct
    21721 tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg
    21781 atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcttac
    21841 acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc
    21901 aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct aggagaaaaa
    21961 tctgaacaaa ttttactagg aatgaaaaca tttatcttcc aacgaggaaa ccaattttta
    22021 ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg atttgggatt
    22081 gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac
    22141 gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat
    22201 acattgactt catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat
    22261 cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat
    22321 atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc
    22381 caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt
    22441 tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg
    22501 tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat
    22561 ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt
    22621 atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg
    22681 acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc
    22741 tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg
    22801 atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct
    22861 cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag
    22921 tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccact
    22981 tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa
    23041 tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt
    23101 ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc
    23161 tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc
    23221 ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat
    23281 ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca
    23341 cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag
    23401 atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa
    23461 atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa
    23521 gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg
    23581 tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat
    23641 tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt
    23701 gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa
    23761 tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga
    23821 atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt
    23881 cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga
    23941 agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat
    24001 caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt
    24061 ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga
    24121 agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa
    24181 taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt
    24241 gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa
    24301 agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg
    24361 aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc
    24421 ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg
    24481 tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa
    24541 gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt
    24601 agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag
    24661 atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag
    24721 agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag
    24781 tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg
    24841 gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg
    24901 attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga
    24961 aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc
    25021 ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc
    25081 tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag
    25141 cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa
    25201 aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg
    25261 gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg
    25321 cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag
    25381 atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt
    25441 tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta
    25501 tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag
    25561 tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa
    25621 cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga
    25681 caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc
    25741 tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa
    25801 gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg
    25861 aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag
    25921 aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa
    25981 aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag
    26041 ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag
    26101 aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac
    26161 gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa
    26221 taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt
    26281 atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc
    26341 aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc
    26401 tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac
    26461 ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt
    26521 ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga
    26581 tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta
    26641 tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg
    26701 atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag
    26761 gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa
    26821 cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca
    26881 ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga
    26941 atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat
    27001 cagtctggca actaattctg cattggaaat agcagaagga gaatttatag cactcttaga
    27061 taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca
    27121 tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt
    27181 tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc
    27241 tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta
    27301 tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca
    27361 tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag
    27421 taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct
    27481 gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat
    27541 tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta
    27601 tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga
    27661 aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata
    27721 caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc
    27781 tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa
    27841 cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga
    27901 tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg
    27961 agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca
    28021 ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg
    28081 tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt
    28141 agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta
    28201 tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc
    28261 agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata
    28321 tattgcgcat gatccatttt acaatgataa tttaacaaaa gaaggaatcg gcgacttttc
    28381 gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac
    28441 ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg
    28501 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    28561 caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt
    28621 cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct
    28681 cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa
    28741 tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca
    28801 aaagccactc tatcaattat catgggttta aaataagaag tgatggagat aaccagattg
    28861 ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc
    28921 tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca
    28981 ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta
    29041 tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac
    29101 aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga
    29161 gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg
    29221 tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta
    29281 cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg
    29341 ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt
    29401 gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag
    29461 tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa
    29521 taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc
    29581 agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa
    29641 ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata
    29701 tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc
    29761 actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg
    29821 taatacaggt attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata
    29881 taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta
    29941 ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt
    30001 agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac
    30061 taggttttta aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt
    30121 atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga
    30181 tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat
    30241 cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat
    30301 tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt
    30361 gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc
    30421 gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct
    30481 tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa
    30541 aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc
    30601 aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat
    30661 caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga
    30721 aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac
    30781 atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat
    30841 tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat
    30901 catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt
    30961 agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca
    31021 aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg
    31081 accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc
    31141 tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt
    31201 aggaccacgt cctgaacgtc ctttcttcgt cgatcagttc aatgcagaaa acccttatta
    31261 ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata
    31321 tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt
    31381 gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc
    31441 tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga
    31501 aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt
    31561 accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga
    31621 ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat
    31681 cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg
    31741 gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt
    31801 cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa
    31861 gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag
    31921 aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt
    31981 tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg
    32041 ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata atagttgtga
    32101 ggatcgaata tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa
    32161 agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa
    32221 caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag acgatattaa
    32281 taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta
    32341 tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga
    32401 tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac
    32461 aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt
    32521 cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga
    32581 ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga
    32641 gtctttcatg atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt
    32701 gatttatgaa gattatgcag atgttcaaga ctatcattca gagattgaaa gattaggagg
    32761 ccgtattttt gttgtaccta atcctaagaa aaatattttt aaatatttaa tagaagtgaa
    32821 taagttacta aaaaaagaat ctttttctat agttcacaat caggtttatt ttggcggggg
    32881 aattaattta tggttagcca aaaagaatgg aatcagacag agaattgctc atagccatgc
    32941 aactgaagat ggaaaatctc aaaatattgt aatgaatgta ttgcgtaaat ttttaaccaa
    33001 attgttgtta caaaatgcaa cagactacct tgccgttagt caagaagctg gtgaaagtct
    33061 gtttcaaaac cacccctttg agatagttca taatggtatt gatttagagc tttatagtaa
    33121 aaattcagaa gctaaggtta ataagagaaa agaattggat atctcaatga gtacttttgt
    33181 tgtgggaaac attggacgat tagagaaaca aaaaaatcaa tcttatttgt tagagatttt
    33241 tttagaaata ctaaaaatga attctgattc ttacttgatt ttagtaggtg aagggtcact
    33301 tagaaataaa ctgaaagcca aagcagtaga attggatata gagaaaagag taaaattttt
    33361 aggagaaaga aacgatattc ccgagttatt atctgtgatg gatgtttttg taatgccttc
    33421 tctttatgaa ggtctgccaa tggttggatt agaagcacaa gcagcaaaat taaaattagt
    33481 cttagcagat actatttcca aagatacaaa gttaacaaat aatgtggatt ttgtatcatt
    33541 agatgaaaca ccacaagaat gggcaaaaaa agtaataaaa gagccgtatg ctaataaaat
    33601 aacacaagaa ctaaagagtt atgatgtttc gtacactact gaacaaatgg agagtatata
    33661 tagtagagat cattaaaaat gttcaagata ttttaggagt gattattttt gaaaatatgt
    33721 tatttgatat tggcatacaa tgatattggc aacttaaaga gattggtcaa tcgattgaac
    33781 gaaaatgcag tttttattat tcatatagat aaaaaaactg atatagaacc atttattgaa
    33841 gcatttaaat catatgataa tgttgaattt ttatgtaata aaaaccgtgt taaaatttat
    33901 tggggtggtt tttccattgt acaagcagaa atcaatttag taaagagagc attacaaaat
    33961 gagaaatatt taaaatatgt attattatct ggtgctgatt atccaatcaa agataatgag
    34021 tatatttata attattttaa aaagaatagt tcagtggagt ttattcgtgg aatagatcta
    34081 gatcaaatta aacataa
//
LOCUS       DASJSZ010000025        32790 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.025, whole genome shotgun sequence.
ACCESSION   DASJSZ010000025 DASJSZ010000000
VERSION     DASJSZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.025"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            190..1464
                     /locus_tag="U4B03_001098"
     CDS             190..1464
                     /locus_tag="U4B03_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2067968.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1847..2575)
                     /locus_tag="U4B03_001099"
     CDS             complement(1847..2575)
                     /locus_tag="U4B03_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEN2067969.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(2553..4127)
                     /locus_tag="U4B03_001100"
     CDS             complement(2553..4127)
                     /locus_tag="U4B03_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN2067970.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            4356..5294
                     /locus_tag="U4B03_001101"
     CDS             4356..5294
                     /locus_tag="U4B03_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2067971.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            5306..6217
                     /locus_tag="U4B03_001102"
     CDS             5306..6217
                     /locus_tag="U4B03_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2067972.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            6241..7713
                     /locus_tag="U4B03_001103"
     CDS             6241..7713
                     /locus_tag="U4B03_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2067973.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            7764..8375
                     /locus_tag="U4B03_001104"
     CDS             7764..8375
                     /locus_tag="U4B03_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2067974.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            8405..10318
                     /locus_tag="U4B03_001105"
     CDS             8405..10318
                     /locus_tag="U4B03_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEN2067975.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            10446..13427
                     /locus_tag="U4B03_001106"
     CDS             10446..13427
                     /locus_tag="U4B03_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEN2067976.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            13424..15085
                     /locus_tag="U4B03_001107"
     CDS             13424..15085
                     /locus_tag="U4B03_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEN2067977.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            15125..16057
                     /locus_tag="U4B03_001108"
     CDS             15125..16057
                     /locus_tag="U4B03_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEN2067978.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(16449..17996)
                     /locus_tag="U4B03_001109"
     CDS             complement(16449..17996)
                     /locus_tag="U4B03_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEN2067979.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(18098..18451)
                     /gene="tnpB"
                     /locus_tag="U4B03_001110"
     CDS             complement(18098..18451)
                     /gene="tnpB"
                     /locus_tag="U4B03_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEN2067980.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(18441..18635)
                     /locus_tag="U4B03_001111"
     CDS             complement(18441..18635)
                     /locus_tag="U4B03_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067981.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(18861..20063)
                     /locus_tag="U4B03_001112"
     CDS             complement(18861..20063)
                     /locus_tag="U4B03_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2067982.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(20066..20260)
                     /locus_tag="U4B03_001113"
     CDS             complement(20066..20260)
                     /locus_tag="U4B03_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN2067983.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(20307..21155)
                     /locus_tag="U4B03_001114"
     CDS             complement(20307..21155)
                     /locus_tag="U4B03_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEN2067984.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(21360..21710)
                     /locus_tag="U4B03_001115"
     CDS             complement(21360..21710)
                     /locus_tag="U4B03_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2067985.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            22305..22751
                     /locus_tag="U4B03_001116"
     CDS             22305..22751
                     /locus_tag="U4B03_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN2067986.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            23375..24367
                     /locus_tag="U4B03_001117"
     CDS             23375..24367
                     /locus_tag="U4B03_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2067987.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            24383..25267
                     /locus_tag="U4B03_001118"
     CDS             24383..25267
                     /locus_tag="U4B03_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2067988.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            25316..26923
                     /locus_tag="U4B03_001119"
     CDS             25316..26923
                     /locus_tag="U4B03_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2067989.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            26924..28408
                     /locus_tag="U4B03_001120"
     CDS             26924..28408
                     /locus_tag="U4B03_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEN2067990.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            28585..29577
                     /locus_tag="U4B03_001121"
     CDS             28585..29577
                     /locus_tag="U4B03_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2067991.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            29629..30498
                     /locus_tag="U4B03_001122"
     CDS             29629..30498
                     /locus_tag="U4B03_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2067992.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(30918..32483)
                     /gene="guaA"
                     /locus_tag="U4B03_001123"
     CDS             complement(30918..32483)
                     /gene="guaA"
                     /locus_tag="U4B03_001123"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN2067993.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(32538..>32790)
                     /locus_tag="U4B03_001124"
     CDS             complement(32538..>32790)
                     /locus_tag="U4B03_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2067994.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT        10280 a         5520 c         6603 g        10387 t
ORIGIN      
        1 aaataggact gatagagtga tataagaaca tctaaggtag aattgatttt gcttctataa
       61 tattttgtgt tggtggaaaa tcgaaggcga ttttctacta acactttttc ttcgtttcaa
      121 ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca aaacgtcatc ctaccaaagg
      181 agtctctcaa tggataatca tactagaaaa ttactcggat taacagataa acatttattt
      241 tttgatgaag aatggctgat tgaaaaagaa tataaaggcg ttcaagcgca gtttattaaa
      301 ggaaaattgg atgactctcc ttcctattgt gaacactgtc gatcggttcg tattatccgc
      361 aacggttcct atacgacacg cactcagata ttgaaagtca aagaaaagtt aactatttta
      421 gaattaaaac gtacccgttt tctctgccat gattgtggaa ggaccttttc tgcgaaaacg
      481 gacttagttg atgaacatca tcaactaaca aaggaactga agcaggcaat tctcatggaa
      541 ctgtacgaaa atcaatcacg taaactaatc gctaagaagt acttcgtctc ggatgggacg
      601 gtgacacgga tcttgcgtga agcaacgaag cattaccagt cacgtatgaa cttccttccg
      661 acggtgttgt gtatggatga gttcaaatca atgaagtctg tttctggttc catgagtttt
      721 atttgtgttg atgggacaac ccatcagcta tttacgatcc ttgaagatcg cagactttac
      781 aaactaactc aatacttcct gcactttcct agaaaagccc gtttaaaggt caagtaccta
      841 gtgatggaca tgaatgccag ctattgtcag ttgcttaaaa cagtctttcc aaatgcggag
      901 atcgtaactg accgctttca tattgtccaa cacatcaatc gcagcttcaa tcaattacgt
      961 gtccagatca tgaatcgttt tcgaacctca cactctgaaa atcagaagaa gtaccgtcgg
     1021 ctcaagcgat attggaaact cttactcaaa gattccacga catgggagcc actcaaacgg
     1081 cattatcatc gactgtttaa acgaccaatt agtcaaacag aaatcgttga tgagctgtta
     1141 agctacaacg aagaattgcg aactgcgtat catttttgtc aattattacg ctattatttc
     1201 gttaaacgag gtacggaagg ctttcaagaa acccttaaaa caatttcatc tacgttacct
     1261 cagtcgttta aaaagaaatt cacgatcttc cattgctacc aatcgggtat ttcaaatgcc
     1321 tttaaggtgt cccactcaaa cggcgtcact gaggggctga ataacaagat caagctgatt
     1381 aaacgaattg cgtttggtta tcgcaacttt tataactttc gggcacggat ctatttacaa
     1441 caaggtctga tctttgaaaa ctaaaaaaat atgcggcgca cagaacgcgc cgcatatcgg
     1501 actaagtttt attcaaattt gtcttcacca acacgatttg acgaagagcc tatttaaata
     1561 actacatcca tttattctat cttctaaaat agcctttttc atttcagtta aatagttcag
     1621 cgccattttt gcactcgact tgcatagtag ttttttccat attcctcaac ctcagcgggg
     1681 gttgaagaca taaggaaaat cctatgcgtt atgctcaact ggatctgatt aaatattcta
     1741 atgtattttt ctctaccatc attaaatggt taatagccaa aaatcaccac ttttcaatgg
     1801 ctccttgttg tataagtata actagaagat gaacacgtct gagaaattat agaagctttt
     1861 tacggtaatt actggggctg tgtccagtag tttttttgaa gagaacagaa aaatagctca
     1921 cgtgatctat tcctactttt tgactaattt catcaataga taagaatgtg tctttcagta
     1981 gagcacttgc tcttttgatt cgaaagtcta gcaagtaatc atgaaaggaa taacctgttt
     2041 cttttttgaa ggcccgtcct aaataagcgg gatggtaatg gaattgtgta gctacatctt
     2101 tgagcgtaag cttttcacta taattgtttt gaacgtaatc aatcacttgt tgaattgact
     2161 cattaaaaga atcatcagtt agagagtttt gattagctgc tgatttacgc attttttctt
     2221 cttgtactaa cttaactaaa taaggaaaat caatgggttt cgttatgtaa cttttaatag
     2281 ataaatcaat cccctttttt gcatactgaa aattttggtg ggcactaatg acaatactag
     2341 taaagtagcg attggatgtt tgttgcaaag attcgattaa ttcgagtcca gaaattttcc
     2401 ccatcgtaat atctgtgata agtaaatcaa agcgctggtt ttgcaggttt ttaaatgctt
     2461 cgtctccgtc tgtgtagcag tgatcgataa tgatgtcggg gactttcttc aaaagataat
     2521 gccttatgcc ttctacaatt gtacaatcat cgtcaacaat tgcaattctc aacatgcata
     2581 ttcccccctt tttgtatttt gatagtaatc gttgttccga cgtgtagttg gctttctata
     2641 acaaaacgtg tatctccagg atagagatgc tttatacgtc gaaaagaatt tgagatgccg
     2701 atattctcac tataaggaat atcatcatta agtttttgtt gaatatgctc cagcttgtct
     2761 tggtccattc ctttcccatt ttcaacgatt tttaacacat aatggttggt tgtttcagtt
     2821 gcatagacag tcaaacgatt ttcctttttt gatgaatcga agccgtgttt aatataattt
     2881 tcaacaaatg gctgtaaaga aagttttggt aacattaatt gatcaatatg atcaatcagc
     2941 tgatagtcaa agttatgtcg ataacgacgt tgatgtagtt gcaaggagag gtcgatatta
     3001 attaattcct ctttcaaatc aattatgtcg ggctgatatg tattattgcg atacaacttg
     3061 ctcaataaat atatactgtc gctgatttct tcgtcatcat tcaatatggc agtcattcga
     3121 ataaattcca aattattaaa tagaaaatga ggctcgattt gatattgaag tgctgcatac
     3181 tttgcttctt gcttcaacca attattttca taaagtgttt tttgatatgt agcattatct
     3241 aaataaaact gattgatatg catagcgatt tctttcaaat caccgacttt gttttcgata
     3301 ttgattgact tgtagtctcc tgtagatgaa tgatttaagg aagtaatgat ttcctgatac
     3361 tgctggatat agttacgaaa acttctattt aataattgat gaatagcaag aataaacaaa
     3421 ctcattaata agaaaatcat gattgccaga cctaataagg tttgtgacga ataatttgga
     3481 ttttcatata cttgtaatgt gtggttaccg atagctatct gttgcgtttt tttccattta
     3541 gaaggcgaga tgtcttcaga taaattgacg gaatcaatac cattagacaa agaataattg
     3601 taatgtgttg gaaagagact cttttttagc gcgttgatcg aaacgtctag aatcaatgta
     3661 gcatcgggca tgtcctcact aatgaaactt aatgaacgaa taaaagaaat tttttcgttt
     3721 tttgtaggtg cgtaggcact taagtatttt tcgtaatcta atgagataga tatattttta
     3781 acttcatcat cttgcacaag tttttttaag ttatctacta aataggtagg ggagcgatcc
     3841 tttgacaaaa tatcttccat gtattcagca ggtgatgaga ggtagtagtc attaaggttt
     3901 tcttggaaat cataattttg atagatctct gtcacgatac cggagagaat acgattatat
     3961 gtttccatat agtgggtgat ttgctgaatg ttttgcaggt tttgattttt ttctttttca
     4021 atcacattga tataggaaaa gaacaatgtg aaacaggcaa gtaaagtaat aaaaaaaatc
     4081 aatatgtatg taaaacgcat aataactttt gataaatcgt ttttcattca aagtcctcat
     4141 tttgataatc tctaatttta tgaatcatta tagcacatat tacaagtgat gtaaacgctt
     4201 tgcaaacagg gctacttatg attgtgaata cagtttccgt ttaaaaacta ttctattgta
     4261 gaggtttaat aaactaaaaa taatgtataa aaaattaatg taagcgatta accaagtggg
     4321 tataatcaag gaaacgctga gagaggagag acaaaatgaa gctaaagaaa gggttattag
     4381 gaaatctaaa aaaaaaccga gcattgttta ttatgctttt accaggtgcg ttatggttat
     4441 ttatattttg ctatatacca atgtttggag ttgtaatcgc ttttaaagat ttcagaattt
     4501 catcaagggg attctttaca agtttgttca acagtccttg ggttggctgg gaaaatttca
     4561 aatatctctt tgcttcgcaa gatgcttgga tcattactcg caatacggtt gtttataacg
     4621 ttgtaggtat tttgctaagt actttttgta cagtgtcatt agcaatcatt ctgagccaaa
     4681 tggcgaacaa acggttattg aaaaagatgc agacatttat gatttttcct aattttttat
     4741 catgggtagt catcagttac tttgtttacg catttttgag tacggataaa ggattaatta
     4801 atattttact aagcaatttt ggtgtggaac ccataaattg gtatagtgaa ccgaagtact
     4861 ggccttttat tattgttttt acgagtttat ggaaaggcgt aggctatgga tccatcattt
     4921 attacgcgtc aattatgggg atcgatacta gttattatga agctgcttta gttgatggtg
     4981 ccagtaaatg gcaacagata aggaatgtag tcttgccaca tttaagatcg attattgtca
     5041 ttatgttgat tttgtctgtt ggtagtattt ttcgagctga ttttggattg ttttatcaat
     5101 tgccaagaaa ttcgggggct ctttatgatg tgacgaatgt attagatact tatgtctacc
     5161 gtggattaat gaatagcgga gatatcggaa tgtctacggc agcgggatta tatcagtcat
     5221 cagttggctt tgtcttgatt ttgattacta ataagatagt caacaagtta gatcctagtt
     5281 cagcactatt ttaggaggaa tcaaaatgat atcaaaatta aaacggcaga tgtacttttc
     5341 gctgccagtc gaacggtttt tcacaatatt actaatctta tttactctta gctgtatttt
     5401 tccctttatt ttcgtaatcg tcatttcatt aacagatgaa aaagcattac aactcaacgg
     5461 ttacgaatta ttgccggctc aatggagcac tgatgcatat caatacttaa tacaagatgg
     5521 cggacagtta cttcggtcat taggcgttac aattatgatt acagtgattg gaacgttgat
     5581 tactgttttt atgacgggaa cctatgctta tgttttatca agagcctctt tcccttaccg
     5641 gaagttcttt actttttatc ttttttttac catgttattt gcgggaggga tggttccttc
     5701 ttacttagtg atgacgaaga tgcttggctt aaaaaataca atttgggctt tgattctacc
     5761 tcttgctttt agcccataca atgtcattat tttaaggaca ttctttaaga agtcgattcc
     5821 agaatcgatt atcgaatccg ctaaaatgga tggctgtagt gagtttcgag tttttttcca
     5881 aatcgttttg cctttagcga tcccaggggt tgcaacaata ggtttatttt caagcttagg
     5941 ttattggaat gactggttca acgcattgct gtatattgac accaacaaat tgattccatt
     6001 gcaatatttg ttaatgaaga ttcaaaattc aatggaattc ttagcaaata ataatgacat
     6061 tacattagcg cagcaacaag caattcaaaa tagccttcct caagaatcaa cacgaatggc
     6121 aatggtcgta gtagctactt taccgattgc aattgtttat ccgttcttcc aaaaatactt
     6181 tgttcaagga ttgacgatcg gtggagtgaa agaataaaaa agaataggag atgttaaaag
     6241 atgaaaaaga acatgcagaa atatttgatt ggtggtgctt ctgtactaac ccttggccta
     6301 ttaggtgctt gtggtagcag taatggcgaa gcaaataatg aaacaggaac cacaaaagtt
     6361 acttggtatc aggttggaga agaacccaaa gattttgatg aggtcatgac aaaagtcaac
     6421 gaaaaattaa aggaagaaat caatgtagag ttggatatgc ggctgattcc ggacggggat
     6481 tatcagcaaa agcttggtgt gatgatcaat tcaggagaag aatatgatat ttgtttcgtc
     6541 aatggcacag attatgtgaa ttatgggaac aaaggtgctt tcattagtct caacgatcat
     6601 ttggataaag agttgaaaga ttatgcagct gaattgaatg aaggatttat tgaaggtggg
     6661 gcaattaatg gcgaaactta tggattgcca gtcaataacg atattgccac aacggaatat
     6721 tggaccttta ataaagattt ggtagatcaa taccaattgg atgtagagaa tgttgaaggt
     6781 gtagaaacat tggctccagt attagaaaag tttgcagaaa aaaatactga tacgaatatt
     6841 acgccaatcg gtatgacccg taccagtgtc tgtattcctt atgacaatat tatcggtggt
     6901 ggtgtaccgc tagcggttgc atttgaaggc gatacacaaa cagttgttaa tacttatgag
     6961 actccagaaa tgatgactta tttgaaggct atgcatgagt tttataaaaa aggatatatt
     7021 ttgaaagatg cagccactgt tacgacagat ccatttcgaa ttgatagtaa aaactggttt
     7081 ttaggtcgta actcaagtgg ttatcaacca ttagcacaaa aaaaattaga agctctagcg
     7141 ggacatggat taattttaaa accagctgga aaagctttta aaacgactgg atttgcaaga
     7201 gtggcaatgc atgcggtatc tgcttcaagt aaacatccgg aagcagcctt gaaattgttg
     7261 aattatgtga ataccaatca agaagtagcg gatttaatgg tttatggaat tgaaggaaaa
     7321 cactatgatc gcaatgaaga tggttcgatt actttaacaa actctgatga ttattatcaa
     7381 ggcgcttggg cattggtaaa tacttatcta gtttcaaaaa cttcaccgat tacagaagaa
     7441 agtaaaaaac tagaagaaga aagtaaaaag tttcaaaatg aagcaccgat gtctcctatt
     7501 ttaggtttct cttttgacgc ttctgatttt cgaacagaaa ttgcaagtgt acaaaatatc
     7561 gtgtcacaat atgaagtact tttaagctcg ggaacagttg accctgaaca atatgtacca
     7621 gagttgttag aaaaactaga aaaagctgga ttggataaaa ttcaggcatc tgctcaagaa
     7681 caatacgatg aatggcgaaa atctaacaaa taatttttta ctaaatgaaa agaatagact
     7741 aagcagttag tggggggatt caaatgaacg aaaacaaatg gaccaaagga tttaattggg
     7801 tttttcaatt gttgttacta aactttactt attttatact aatttgttta ggatttggaa
     7861 ttttaggttt tttccctgct acgatagctt tgattgctgg cgttgtgaat cgaaaaaaat
     7921 atggcttgca agagtctgtg aagttcatga gtaaaatcta taagaaaaat tttgttagat
     7981 ataatctata ttgctggtca gcaactttgt tgttggccgg caatattact acgatctggc
     8041 tttgttggca gcaaagcgca ccattgtttt atttattaag tacattatta tcggtaatta
     8101 cactgttcct agtctttgca atgttgaatt gggtcgtgat caaaagtctt gggttgtgga
     8161 tgatgggaga ccgactgatt gactatttgc ttttcacagc aacgatgttg ccaacggtag
     8221 tcatgcaagg cttggtgctt ggaatttatt tccgaatatt gatgattttg cctgtgattt
     8281 ttctgtttgg cggaacagct gtaacggtaa tattgattag taaattaact gaaagagcgt
     8341 tagcaaagtt gccttgtcag atacaatcgc actaattttt tcacaaaaag gagtatttct
     8401 ttctatgaag ttaactgata aacaagtagc taatatagcc tttatccagc aacttgagat
     8461 tccattgaca caaaaagatg aagaacaaat agacttgatt tttactgaca caaaggctaa
     8521 tgtgcgacaa atcaagatta ctaaaaatga gcagcaacga atttgcttgt cttattcaca
     8581 tgaagacttg ctgtatcgag gattgtttat ctgccttcca cgttttttag ctcaaggagg
     8641 agagatgcca tacattgaag aaacctcgat cattgagacc agttattcat tagatgcttc
     8701 tcgtaatggc gtgatgaata aagagactct aaaaaaattc atttcttact tagcgttgat
     8761 ggggtacaac acattgtata tttacacaga agatacctat gaagtagaag accttccata
     8821 ttttggttac cttcgcggag cgtatacaaa agaagaagtg aatgaaattg ttgaatttgc
     8881 ttctggtttt ggcatagaag tagttccatc gatccagacc cttgctcact tgacccaatt
     8941 tttgaagtgg tatgctcaac aagaactgat ggatgatcaa aatactctac tgatcggtga
     9001 agaaaaggtt tatgcggcaa tcgaaaagat gattattgcc tgcaaagaaa tgtatcagac
     9061 agagcgtatt catttgggaa tggatgaagc ctatcaagca ggtctgggac gatatttaag
     9121 cctgtatggg tattgtgatc ggacaacttt aatgattcaa catatggacc gtgtgttatc
     9181 aattgttgat aaatataaga tgaaaccaat gatttggagt gatttcattt ataagctatt
     9241 agatgaaacc ggtaaagata ggtattacaa tcctgatttt aaactcaaag cattagcagc
     9301 taaaggatta ccagcaggat taacctatgt tcattgggac tatggttcag atgaagtttc
     9361 ctcctatgag aaagtaattg ataaccattt gaaattttgt gacattgatc attacgtttt
     9421 agctagtggt gcccatatct gggggagact agccccgaat catgggaaaa cagaagcaat
     9481 tgtttctgca gctgtaaaag cttctaaaaa taaaggtgta cgttctgtga tgttaactac
     9541 ttggggagat gaagggcagg aaacagagca ttggcattcc ttaattgggg cattaaaatt
     9601 agcagattat gtctattctg aagaagagtg gaatgatttt gaggaaatct acgatcgttt
     9661 attaggaaca ggtagttatc aattaatgtc cgaattacga tatttcgatg aagtggatac
     9721 cattacaaat aacaacttac aaatggcttc cgtgagtaaa ctgctacttt ggcaagatcc
     9781 gttagttggt atatatgact ttcatgtagc cgctcataac aaaattgcca ctagaacaat
     9841 tggtacttat tatcatgagt tagctcagaa attagatatg aataaaaatt ggaatccaaa
     9901 acaaccaatt ttaacaacta ttcgtcaacg atatatactt ttagctgaaa ttttagcaca
     9961 aaaggcagaa ctaggcatct atttacaaca ctgctgtctg gtagaagacc ttgcaggatt
    10021 aatggaatta gcgaatcggt gtgaagcact agccaatcga gtaaatgaat tggcatctat
    10081 tcatgaaata atttggtatc aaacatacaa aacctatggt tgggaagtat tagaacaacg
    10141 atatgctgca ttagctcatc gtctgaaaac cgttgcgcgt cgtattaaaa atcatcttca
    10201 ggggcaagat gccctagagg agcttttgga agaacgatta ccgttttgtc aatatcaagt
    10261 gccgcttgaa atttctggct ttaattatcg gcaaagctcg gtgtcaggct acaattaacg
    10321 aaggaatctt cccgttgaac ggaccacttg ctcatctaat gggaaagtgg ttcgttttct
    10381 attcataagt agcttaccac taactctttt attaagaaga gaaaaaaagt aggaagtgag
    10441 aaaatatgtt atcggactat tatcaaaatc tgtcagttat aagtgtcaat acagaaccat
    10501 tacgtaatta ttatattcct tatgcaagta aagaagaagc aaaacaagag ttagaaaggg
    10561 aaaaaacgag ccagtttatc gatctcaatg gtacatggga ttttcagtat tatccttctg
    10621 tccaacagtt accggaaaat ttttttcaag aggcctttac tgaaagctat gatagaattt
    10681 ctgttcctgg agtttggcaa acagagggat ttgacttacc tcagtatatc aatgtatctt
    10741 atccaattcc ttttgatccg ccttatgtac ctaatgatac cccttgtggt gtatatcggc
    10801 gtcagtttca aattgagcaa acagatgcac gcttttactt aaatctagaa ggagcagata
    10861 gtaattgttt tgtttggata aatagaaact ttgtaggcta tgaccaagtg agtcatagta
    10921 catctgaatt tgatgtgacc aagtatttac aggttggggt taatgaaatt caaattctag
    10981 tgatgaagtg gtgtgatggt acctactttg aagatcagga taagtggcga atgagcggtc
    11041 tatttcgtcc agtgtatctt cttcaacgac caactatgca tctgacaagt tatcaactac
    11101 ggacgttgac tgatcctaaa aaaggaagcg gaacattaat ggtttctttt caacgattta
    11161 ctcctattga agtatcgtta cctatttctt acgaattatt ccatcctgat ggacgtctca
    11221 tagcgaaagg ggagtgttac tcggaacaac aaataaattt ggatgttgaa gattgccttt
    11281 tttggacagc agaaacgcct aaattatata cattactttt aacatgtaat ggtgaatatt
    11341 ttaaagaaaa agttggcttt cgagcactga cgatcaagaa tcgtaagtta ttgctgaacg
    11401 gaacacctat taaattatat ggagtcaact atcatgacgt gaatcagcaa ggcacgccag
    11461 tgatgacccg agagcagtta cgtgcagatt tgttattaat gaaaaaatta aatgtgaata
    11521 ctgtacgaac ggcacattac cctaaatcac cagattttta tgaattggcg gatgagattg
    11581 gtctgtatgt aatcaatgaa gcagatttgg agtgtcatgg tgtggttaat ctatatgata
    11641 ctgaagcgaa ttatcaacta attgctaatg atccgacatt cgaaaagccg attatggacc
    11701 gagtgatccg ttctattctt ccttatcaaa atcgaagctc tatcattatg tggtctatgg
    11761 ggaatgaatc tggttatgga gtaaattttg aaaaagctca agctttagca cggaaattgg
    11821 atgattcacg gttgattcat aatgaacgct cgattgaacc agttattgac caagtgaatg
    11881 attttagtaa cttggatgtt attagtcaaa tgtatccccc actggaagat gttcatcgtc
    11941 attgtcaaag aagtgatact gaaaaacctt tttttatgtg tgaatatagc cacgctatgg
    12001 gcaacggtcc tggagatctt aaaacgtact tcgatttgat tcaacagtat gattcctttc
    12061 ttggcggttg tgtctgggag tggactgatc atgcaatcaa tagaggaact ttagaaacgc
    12121 catcttattt atacggtggt gattttggtg aaaaatatca cgacggcaat ttttgcgtag
    12181 atggtttatt gaccccagat cggcaattaa cagtaggtgc attggagtac cgtaatattc
    12241 attcgcctat tcaactgatt gctgtagatg aggagctgca agaagtaacc ttgagcaata
    12301 taactgcttt tacagccggt tcagcattta acctttctct tgaatggtat atagatggaa
    12361 aaaaaacagt ttatgaagta atgaatcctt tcatacttga gcctggcaaa agttgttcat
    12421 tgcccatcac agtacctaca acgattcatg gtgctcatac ggttgctgtt tcgctatctg
    12481 tttataaaaa tgatgagctt tatggacagc aacagctctt tcttaaaaaa gaaaaaatga
    12541 gattgccaag aactgctcat cataatgatg caccacaaat ttcagtaaaa ggcacagagc
    12601 tgataatcaa aggagaatcc tttatttata tatatcaatt agcaaaagct gcgttcactt
    12661 ctgtaaagat caatcaaaag gaacttttgg aacgtcctac tcaattttca atttggcgtg
    12721 caccaacgga taatgatcgt aacttagtaa acaaatggaa agcagctgga tatgctgaaa
    12781 tgtctttaga aatcagaaaa aaagactgga aattcgtaga tgatcatctc ctgatcgaaa
    12841 cgtcttatcg aggagttatt ccaggattac agccaatttt tacaatgcac atacagttga
    12901 aaataaatgg tgcaggcgta atcaaccaaa aagtatcagt ggagaaaaat ccgatttttc
    12961 cagatttccc tcgctttggg atggactggc cgttgaatga tactttttcg aaggtttctt
    13021 attgtggcta tgggccgtat gaaagttatc ctgataagca ctatgctgcg atgtttggta
    13081 tttatgagat ggagattgag gaatttgatc agccatatat caaaccacaa gaatatggta
    13141 accgctggca atgtgattgg gtatcaatca aaaatcatca aggatctacc atgacggttt
    13201 atggaaactc taatttttct gctctgcctt atagtcaagc tcaattaacc gaaaagtcac
    13261 accattatga attaataaaa gaaaatagaa cttggttcaa tttaagttat aaacaaaatg
    13321 gtatcggctc acacagttgc ggtccttatt tagaagatcg tttccgtttt atggaagata
    13381 cttttgactg gaatatagtg attactttca aaggagaaaa tagatgaaag aaacaaagat
    13441 caatcaagtc aaaaaaaaat tatctcgtta tcaagaagtt gtttatcgaa accattgctt
    13501 aaaatggtca aaaaataagc tgaatgttcg ttttaatgga ttattttatc gtttcgaaca
    13561 gttagaagaa gtctggaaac cggtaacgaa tttccaatca tgtcctgtac agctcataaa
    13621 tgatccaaaa gaaacatacg atttatgtat aacacacctt tataaccagc atttgaaaaa
    13681 tgacggattt tatttaaagg ttactaatga caatcaactt acgattgaat cagcaaacct
    13741 tcgagggttt caatatgcta tggaagcctt attagagagt tttttttcgg taggagatca
    13801 attattatta ccggaattga ttctgaaaca tgaaccatca gtgaaaattc gaggaatcat
    13861 tgaagggttt tatggtatcc cctggacaca tgaaatgcgc ctctctcttt tttcatatat
    13921 gaaagataac caattgaata cttttatgta tgcccctaaa gatgatgaat tattacgtaa
    13981 aaaatggcgt gaactctacg atgcgcaaga attagacaaa tttaaggagc ttttatctgc
    14041 tgctgaggct agcaatatcg atttttggta cttaattagt cctggaaatg atattgatat
    14101 tacgtgtgaa gaagacattc aagttcttct gaaaaagtta gaacaactga ttgagcttgg
    14161 cgtatttcaa tttggtctat taatggatga tattgactat caattaaaag gtgctgctaa
    14221 acgccgtttt cgagagccag cattcgctca tgcctattta gtgaatcgag tagaggaata
    14281 tcttagcact cggttgtcca atcatgaatt agtcatttgt cctacagaat atgataatcg
    14341 tcatggttct cgctatttag caacgttaag tcaagaaata ccagaacaat taccgttctt
    14401 ttggactggc ccaagcacgt tagcggcgtc aatttctaca gaagagctgc aggaaatggc
    14461 ttctgtctat caacggccga tgctgatttg ggataatatt ccggtaaatg attatttaga
    14521 ggataaagaa ctattgttca tgagtccata tgagaatcga acaccgaatc tttctaagga
    14581 gagataccag gttacaggag tagtttcgaa tccaatggct caattagaag cttcaaagtt
    14641 tacgatcaat agtatggcta attacttatg gaactgtgag cgttttgatc ctttggaaac
    14701 ttggacgtca gtcgttgaaa aagtagttgg aagtaaacta caaccagctt atcttacctt
    14761 aaccaatgct tttccaaatc attacacaag cagtctgctt tcagatgaag aattactgct
    14821 agccaaagac ccagaatggg tcacaaaaga aatggctgct ttagtccaag cagtaaaatg
    14881 gatccagcaa gaacaacata catctagatt gagaacagaa ttggagcctt ggttacaagc
    14941 aatcacagaa agttggactt tctggcaaga atggcaatta aaaaaagatc caaaagaagc
    15001 agaagaatgg ttggctaaga aaaaaacaca ccgcatagga cgtgatttag taactgccca
    15061 tttagagcaa attatcaaaa gttagatatc ttaaaaaata aaaagcaaga aaaaggagaa
    15121 caaaatggtc aagagattaa ttagtgcaaa ctacagtgat gtcaagaaaa tgacgtcaaa
    15181 agagttgaag caatccatca aagcgagtga aggacgggtg attgtttgtg aaacggttgc
    15241 taatactctt ccacaatctg gagaaattac gaatgcagaa gttgcgtcag catttggtgc
    15301 agatttgatt cttttaaatg gtttcgattg ttatcgtcct gcagtttttg gactaccaaa
    15361 tgtgacgatt gagaaggttt ttgatctaca gcaaacgatt gaaaacccga ttcctaaatt
    15421 aaaagaacta gtcggtcgtc ctgtgggaat taatttagaa cccattgccc ctaaaacaaa
    15481 tatgatgagt gagaaagtag aaatcagcca aggaagagca agctctgttg aaacgataca
    15541 aaaagcagaa gaactaggag tcgattttat ttgctttacc ggtaatccag gcactggagt
    15601 aaccaattta gaaattacta aatccgtaaa aatggcaaaa aagtattttt cgggattgat
    15661 tattgctggg aaaatgcata gcgccggctc agaagagcca gttgtgaata aagaggcgat
    15721 tatttcttat gcggaagcag gcgcagatat tttactagtt ccttcagtag ggtctatccc
    15781 aggcttttta gagcaagacc taattgaagc ggttcggttg gcaaaaaaat atgatttgtt
    15841 aacgatgtct gcaatcggta ctagtcagga aagtgcccga ccagaaacat tgcggcaatt
    15901 agccattatg aataaaagtt gtggtgttga tttacaacat attggagatg ctggatacgg
    15961 gggattggca ccagcagaaa atattttcga aacctcggtt gcaattagag gaatgcgaca
    16021 taccatcaac tgcgttagcc gctcgatcaa tcgctaacaa aattaaataa aaaatggtta
    16081 aattgattat ttaagtggga acatacttga gatatcagct aacttctgtc actttaaaaa
    16141 agggactgtg acagaagtga aaatagacaa aaaatatccg aacaagagac taggatagca
    16201 gaaaaactat cgttttgcaa cgaggaccaa ctggaccgta ggagcaattc tcttattgtt
    16261 cggatatttc gcttgttcaa ggacttatgt ctgtagtgcc cttttttggt aggaaaaatg
    16321 gtagggaacc atatcatttt taatgaaaat caagcatttc aaaagttgaa aagtaagcgc
    16381 cccatagaac agtacctgta cgtcaaataa cccgacctat agaatagaat cgggaatgtt
    16441 tggcgttttt agttacgaga aaaatgttgt tgtacatcct tgttccaagg caacagttga
    16501 tctaaagttt ctgctgaaag tacgggaaga tttggtatct tatcaaatag aaactctaga
    16561 tactttacag gattcaatcc gttaagttct gctgttttat atacactcag aatgattcct
    16621 gaagttcgtg ctccttcaag acttgtggaa tgcaaccaat ttttccgtcc tatcaccagc
    16681 tctttgatat ttcgctccgc acgattattt gtgagttcta aacgaccatc ttcaaaaata
    16741 ggttcaaaac cacttcgtag tttacacgca tattgaacgg ctgcatccaa cttggatttt
    16801 gcaacaggat ctatcgttgc catccactca taaaaagcaa taaataatgg acgcagttct
    16861 tgttgccgac agcttaatcg cttctcagga gaaaagtctt tccatttctt ttctaggaca
    16921 aaccatttat ccaattgttt gactgcttga gctgcgggaa tatccgtgtt tttctttcct
    16981 tttggaattg cttcgaaaaa tttccgccga acatgcgcga aacaatagac aagtcgtaca
    17041 ctttccacgg cattataccc gctatagccg tcacagtgta aataccctgt aaactccttg
    17101 agaaaagttt tcggaacatc tccagcacga ctttcgtcgt ggtgataata aacgatggga
    17161 tgctgactgt cttttcctgt acaaaactgc cagtaatagg tatctgtttt ggcactttca
    17221 atcacacgat aacttgtttc atccccgtgt agaatctctt cttgattaag atagactctt
    17281 aatcgttctc ctaaagggtc taacgcgttt tgcactgctt taatatgcca attggtgata
    17341 ttatctctcg aaatatccaa cccatgtgtt tgtttccaat agttctcttg ccgataggca
    17401 ggaactttct gaccaaattt catccggatc gtttccgcca ctaaactatt ggaacctaag
    17461 ctattagaaa tcaaaggttt aggaacgatc gcttttttaa aagaagaaag tccttctttt
    17521 tcgcagcttt tacaacaata ggtatgttga taatgggtga gacaatccaa catagcctga
    17581 tggaaacgaa tttcttctcg tacttttgtc cgtccgatat ctttcatctg tgaaccgcaa
    17641 tgttcacaaa attgttcttc caaaggaagg agatgttctt tctctgttat aggtaagtgt
    17701 ttgattttgt ctgctttcgt tcctacaact cgttgatgtc cttttactgg aataatggtg
    17761 gcagctgctt ctggaacact tacagaaatt tcttcatcac taaaaagatt caattgccca
    17821 ttcgtttcct caaagagagt ctctttagag cgtccaaata atttgttggt taaataattg
    17881 accttttcat tcaatagttg gatttcagtt gtaagattct gaatggtttg ttgttgtgct
    17941 tgattggttt tatgggcttc ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt
    18001 tttcaccacc ttgaatcgaa ctgattttgt tacagaatac cacaagaaac gcacaagaaa
    18061 taggggttca acacattttt tgtgttaacc ccccagctta aaaaactcct ttttttcctg
    18121 gcaaaatagc tcgaggttga tcgatactta atccttctaa caaccatcga acctgttggt
    18181 tcgttaaggt tcgaacttcg tctttcgtcc gaggccattt taacttgccg ttttctatcc
    18241 gcttataaca gagaagaaaa ccatcaccgt cccaatagag aagtttgaaa cggtcttgac
    18301 ggttcccgca aaataaaaaa acggcatctt cgtaaagctc gagttgatac tcttgttgaa
    18361 taagactggc aagaccatca attccttttc gcaaatctgt ttttccgcac acaatataaa
    18421 tatgtttggt ttgaagtaaa tcataaagca ttggacaaca gttctttcaa aatagcttga
    18481 agaatataat tgttaatacc gttatagatc aaaacttcag aaccattctc tcttttgatg
    18541 cgcatcgcta acttggcagc cgcgggttta cctttttctg gtgcactctt ttcgtttcct
    18601 aattggacag gaacaatctc atttaattta tacatagaaa acctccagac ttcttgatag
    18661 ctcaagtata tcttgaggtg catttgttag taaggtactg ttctatgggg cgcttacgtt
    18721 gaaaaacgtt gatttatcgg cattccccat ccatattata tttacgtctt attcccactc
    18781 aatatgagta gcctcgtaaa gaagctatta tatcagtgct ttttcagcct attcctcatt
    18841 ttggtaggga aatggtaggg ttagattttt actgcttgat ttccattaaa gtttgcaact
    18901 ttagattttg ttgcacaggt aaatttaatg tttcctgtca tttgttctgc aattccttca
    18961 tcattacggc tccatagatg agcgtagtgc tttaaagtga tttcagggct agaatggcct
    19021 aaccgttgcg acacgaccag aatgtctgcg ttaaactcat tgattaggta gctaacatga
    19081 gaatgacgta atccttttcc ttgtattagg ggtaccttag ctagtttggc ataacgctga
    19141 ataattcgtt gaacagttga acgatagagt gggaggtcag tgtagctaag aataaattgt
    19201 tctacaccat gatttttttg aataactttc cactttttta gcacttcaat tgtatcatcg
    19261 tccagagaaa tgatccgcaa gccgctttca gtttttgtgt aaggtttccg aataaaatct
    19321 ttttgatttg tcatttccaa ggtgtgatga accctcaact ttttacgttt gaagtccacg
    19381 tcattccaat taagtgctaa tccttcgctt acacgaacac cagtcatgta atatagccag
    19441 atcattaaaa aacacatatg ttcgtagaaa tcatctttat aaatttttgc caaaacttct
    19501 tcaaattctt cttttgtcca gtaaggaacg attgattttc cttttggaat tgcttttgtt
    19561 cttttggaaa tattctcatt cagaaattgc agggtgaccg catagtctaa tgatttacga
    19621 aacataccgt acataagtga actgtatgct tgtgagtatc cgctatcgtt caataaccaa
    19681 attcgataat tttcacaatc tcgcacattg atttcacgaa gcttcttatc accgaaaaat
    19741 tcagtaatct gaatcaatcc acactcacga gaactccaag tacttttttg tacgctagct
    19801 ttgtagtgtg gaacgtaaga ctgctccata aattctttgt aagttaaatc ataattcgca
    19861 aatccattta caaccatgta ttcatttttt atacgcatga cttctttgtg tgcttcacga
    19921 gctgatgaga atcgttttcc tagagaatct tttgttcctt tcttctggat cctctttcca
    19981 gtcacttgat ctgttcctaa ctcaactaga taagaaaagc gaccattttt atcttgatac
    20041 acacaagggt attttgtttt catagttatt cctcctgtga aagaggaaca cctataattt
    20101 cttcaattac ttgtcggggt actaccccaa ctcttttatt attataatat ggatatccct
    20161 gatttaccat tcttgttttt gcttgtcgga ttattgtggt agcttgatgt gttttaaatc
    20221 ctagagcctc taaatctttt cgacaaattg tatttttcat tgtataaact cactttcaaa
    20281 gaaaacacta tcattgggta atagtgttat ttctttattt ttgtataatg aacggtactt
    20341 tcttgaaaat ccatcacttg ttcaggactt cccaactcat ttaaagctaa agcattgtaa
    20401 aaaaatggac gtctaactat atattcgctt gtatatagta tatcttgatt cgtggaatca
    20461 tagggtttct ttaccatctt tttggtgaca gttggtagtt tgagattccg ggacttgaaa
    20521 aaagcttttt tcttatgctc tttcaattcc agctctttat cgaaatattt gctgatatag
    20581 cgcccgcgat tttcgatact gtctacatcg atcttgttga tcttaacgaa accctgaccc
    20641 caaatcgtag ttaaccggtt aaatgagata aaggggaaat caaacaagat aatatggtag
    20701 tggattgcac cgcgttgctg cttttcccac gtggcgagat actttaaatt tgctttttta
    20761 gttttataga ggtcataatt cagtcgtttg ataaattttt taaattcatt atttgcatac
    20821 tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa atttcgtttg attgtcaaaa
    20881 ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt gataatgttt ctgtttgcgt
    20941 tttaaactgt catattgtcc ttgagcggat aattcatcat agttttttct tttttcaaga
    21001 gtaatatcat tttcataaat ccaagctaat ttttgttttc gttccagatt tacttcgttt
    21061 tcgatcgttt tacgtttggg agtaatgatt gcttcataat cataaatttc aatatacgtg
    21121 ggagtttcaa tgattttctg attataagct tgcaaaaatc atcactcctt gttgcgtatt
    21181 cgattgtcaa agaaccagtc attttctcct gatgttgatc tataaatcaa gttaaggggg
    21241 aaggcctgcg tagcagccct tctagcctat ggggtgttgg gcggtcttaa cggacagaaa
    21301 cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac ccaacactcc atagtatagt
    21361 tagttttgtg tagtggtacc ttgttttctg ggaatcaaag ttgccgtatc agcggttgcc
    21421 gataacccat aactgacaaa ttgtccattc gagcgtcccc aaggtacaat caccaaattg
    21481 gtaaatgtaa ttatggctaa accttgttgg gtaagttctt ccggcgtgat cacaggatgt
    21541 tcagatttga ctttgacact gattttttcg tagcgaagtt ttggtaggat acattcgtag
    21601 ctccaaccta gaatttcgcc ctccttttgc cagcgagtga cattgacgac ttggtattct
    21661 ttttcgagtg tttcttttgg taaactgaga tcggtaattt ttattggcat cacaattctc
    21721 cttttttata cgaaagttta tacgatttga ttaaaaaata tagatggcca tctacgactt
    21781 gtattatata cgagaactta tacgtagtca agagattttc taaaaaaata tatacgattt
    21841 tttatacgag tgttataatg aaaaaaatga taaggaggaa tttgaatatg tttggtttag
    21901 tacaacctaa taaagtgaag gtgggccaac gaatcaaaga aataaaagac ggaatgtctc
    21961 tctcttttac agaattaggg aatcgattag gtttaaaaaa gccgacgatc agttcctacg
    22021 ttcaaggcta tgcgttggct cctaagagtg tgatcaacca attatctagt atcagcggaa
    22081 aacctgtcgg ctggttttat tttgggcatg tggaagaata tatccgagag tatttgtttc
    22141 tcattggtca gaaaaaaata gtggacgatt atcctgaaat cattgagaaa ataaagcaag
    22201 aattttatac gggcgagttc aaaaatcctg cttgggagaa tgagctaggc tatcctttgg
    22261 aggaatttat ccaagaatgt tttgtagaat attcgagtga aatcatgaag gactatgtcg
    22321 tacagattac gaaagaggta cttgaagaat ccaaaacgct aaacaaacta tcatcaaaag
    22381 caaaagaaga agctgtcacg attctttcat ctgggatcat tgactatatc gagatgagtg
    22441 gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat ggtgaaagaa tcaataaaaa
    22501 cctttgatgt gaaacaagac ctgacattta atgattccta tttgattgga aagttgatca
    22561 atattcttgg tacagatgaa gatacggagc tgtttctttc ttctttatca atgatattta
    22621 ctgataagca tttttccact cgatttggtg gggaagaatt agtcgaaata tttcaatcga
    22681 tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc agatgaattt tatgattggt
    22741 ttgagaagta aaataagaaa agtggcttag attacaagtt ggtgaaaaaa tttttttcag
    22801 cataacatca gtaatctata aaggggttgt tgattatact gtagaagtta agtactcaag
    22861 agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt tcactagaaa atgtactctt
    22921 ttttgtgatt tttcttagtt atagagtttt tttgaaactt taaaaatgag aaaacatatt
    22981 gattttaaag agtgtagaca tagcttcttt tgctaaatag attaaactta ctggtgagtg
    23041 gtcttgaaca tacttttaaa tcacatttta ctgagtttta ttggatttta taattcattg
    23101 gaaacctcgt acttaatctg tttttatcta tcgactagaa tctgtatata ttccagctat
    23161 caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt gtttaaaata ttattatcag
    23221 aatacttaga tacaaaattc tggcacaaag taaaaaaaat attgacttaa aaaaatggat
    23281 atggtaatct ataaacgaaa atagtggtaa cgtttccaaa atgcatcttc gttaaatttt
    23341 tattttttta aacgaaaatg gaaacgtttc taaaatgtat tgggaggaga ttgaattgga
    23401 aaagacattg ggaccaggtg gtgagataag ggcggtacaa cgggataaaa aaaatatttc
    23461 gaagtgggaa aaattaaaga aacagttgcg gcgtgataag tggatgtact tgttgctctt
    23521 accaggtatc atcatgacgt ttatctttaa gtatgtgcca atgtacggtg ctgtgattgc
    23581 atttaaagac tataatccta tgctaggaat tttggaaagt ccttgggtag gactagatca
    23641 ctttagggaa tttatcagtt caccaaattt tacgcttttg ctgagcaaca cattaaaaat
    23701 tagtatttat ggtttattgt taggtttttt tccaccaatt attttagcac tgagcttgaa
    23761 tcaaattatc aataaaaaaa taaaacagaa aatccagttg attctatatg cgccgaattt
    23821 tatctcagta gttattattg tggggatgct gtttttgttc ctatccgcaa atggacctat
    23881 taatgggtta gtagaaaagg ttacggggca accaattatg ttcatgtcta atccagctta
    23941 tttcagaacg atttatattt tatcgggaat ttggcaagga atcggctggt catccattct
    24001 ttacaccgca acgctggcgg gagtcagtac cgatttatat gaggcagcaa atatcgatgg
    24061 ggcaaatatt ttacaaaaga tttggcatat cgatttgcct gcattgaggc ctgtgatggt
    24121 gatcagcttc atcttggccg ccggcggtat tatgaatgta ggatatgaaa aagcctactt
    24181 gatgcaaact tcaatgaaca tccctgcctc agaaatcatt tccacttatg tatacaaagt
    24241 cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt ggtctgttca atatgattat
    24301 taatgtcgtt ttattactga ttgtcaacaa tataaccaag cggctaaacg atggtcaagg
    24361 cttgtaatga aaggagaaaa ttatgaatag tgctttatca aaaatcgacc gtcaaatctt
    24421 atggttgaat cgagtgattg taactttttt agtcgtcatc accattgttc cgctgattta
    24481 tgtactggta gcttctttta tggacccagc tactttgatc aataaaggta taagcttcaa
    24541 tccgaaagat tggacaatac agggatatca acgggtattt gcagatgatt ccattttacg
    24601 cggttttgta aattcgttgt tttactcttt tagcttcagc ttattgactg tatttattac
    24661 gatgatcact gcgtatcctt tgtctcgacc taacttggcg gggaaaaaga ttattatgac
    24721 tttttttgtc attacaatgt tcgtgggtgg tggcttgata cctacctatt tattggtaaa
    24781 gaacttgggc atgttagata gtgtatgggc gattatcgta ccaggtgcta taaatgtgtg
    24841 gaatattatc ttagcccgta cctattttag tgctttgcca aaagaacttt ctgaagctgc
    24901 tactatcgat ggcgccaatg acttgcaaat actgttcaaa gtcatgcttc cattagcgaa
    24961 accaattatg ttcgtattgt ttttatatgc ctttgttggg caatggaact catactttga
    25021 tgccatgatt tacatcaaag atcccgactt acagccgttg caactcgtat taagaaatat
    25081 tttgatccaa aaccaacctt cacaagacat ggtgggagcc aatactgcca tggcagaaat
    25141 gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc atttctagtc tgccattaat
    25201 cataatgtat cctttcttcc aaaaatattt tgataaaggt gttttagttg gttctgttaa
    25261 agggtaagta ttgttcacta tttgtatgaa taaaaattat aaaggagatt ttaagatgaa
    25321 agcaaaatgg atgctgccaa tcgtttttac ttccgtacta attttggggg cttgtggagg
    25381 ctcaaaagaa aagagtaaat caagccctga ttatgaacta gaaaatgtct cttttccatt
    25441 gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc cctttggctc ccaatgatcc
    25501 taatgacaaa ttgattttcc aacgattaga agaagagacc aatctccata ttgattggac
    25561 aaactacagt agtgattttg ccgaaaaaag aaatttggat atcgctagtg gtgatttacc
    25621 agatgccatc ttcaacgccg ctgcaggtga ctatgattta ttaaactggg cggaaagtgg
    25681 cgttattgta ccgattgaag atttgatcga agattatatg ccaaatctga agaaaatttt
    25741 tgatgaaaac cccgaatacc gacaaatgtc tactgcacct gatggtcaca tttactctat
    25801 gccttggatt gaagaattag gcgaaggaaa agagtcgatt catacagtaa atgggatggc
    25861 ttggatcaac aaggagtggt tggataattt gggtcttgaa atgccgcaaa ccactgatca
    25921 gttgatgact gtgttggagg ctttcaagac gcaagatcca aatggtaatg gtgaagcaga
    25981 tgaaattcct tttagcttta ttaacaatgg aggaaacgaa gacttaaaat ttttaatggg
    26041 ggcattcggt ctaggtgata atgatgacca cttagtcgtt gataacgatg gtcaggtaca
    26101 atttactgct aacaaagaag ggtataaaga agccatcaaa tatttcaatg aaatgaataa
    26161 taaaggtctt attgataaag aggctttcga acaagattgg aatacgtatg ttgccaaagg
    26221 gaaagaacat aagtatggcg tgtactttac ttgggataaa gcaaatatca cgggtgcaga
    26281 tgatagttat gacgtcttgc cagtcttagc gggaccagat ggaacgaaac atgttactcg
    26341 tacgaatggc atgggtttct caagagatcg tttcgtaatt actagtgcga ataaaaactt
    26401 ggaacttaca gctaggtgga tagataaaat gtatgaaccg ttacagtctg tccaaaataa
    26461 ctggggaacc tacggtgatg aagcacaggc gaatattttt gaattaaagg atggtatgtt
    26521 aaaacatttg cctttagagg ggacagcacc gggggaacta agacaaaaaa ccgaagctgg
    26581 aggaccatta gcagttcttg attcttatta cggcacagtc actactatgc cagatgatgc
    26641 gaagtggcgt ttagatttga tgcatgacac gtatttgcca tatattacag aagacaatat
    26701 ctatccacgt gtctttttag aaaaagaaga aacagatcga ttagcaaaaa tcgaaacgga
    26761 tatgtgggat tacatctatc gcaaaagagc agagtggatc accggcggta aaatcgatga
    26821 agaatgggaa gaataccttg ctgaattgga acgtgtaggt ttggaagaat ggattcaaat
    26881 aaaacaaact ggctatgata attttttgaa gggagcacaa taagtgttag tagaagaaac
    26941 ggcgattttg gataaagcaa atcgatttat tgaaatgatg cgaggaaaag ttaaccctca
    27001 gtatcgacaa aactatcatt tttccgcacc catcgggtgg ataaatgacc ccaatgggtt
    27061 tatatattac aaagggcatt accatctctt ctatcaatac tatccttacg atagtcgatg
    27121 ggggcccatg cattgggggc atgcgcgcag tagtgatttg gtccattggg aagatctacc
    27181 agtagcgtta gccccagatc aaccttatga cagcggcggg tgtttctcag gaagtgcaat
    27241 tgaaagagac ggaaaactat atttatttta tacaggctgt caagaaattg atggtattag
    27301 ccatcagcat caatgtttgg cggtctctga agatggtatt cattttagca aatacagtgg
    27361 taatcctgtg gtagcagaaa aacagctagg tgaaaatggg ctaatacaag aatttcgtga
    27421 tcctaaagta attgttcaca acgatggtta ctatatggta gtagctacta aatctacaca
    27481 gtctactggt agaatagtga tgtttcaatc gtcagatttg tatacttggc acttttactc
    27541 tgtgctgcta gaagggactt cagagcaagg aataatgtgg gaatgtcctg atttattcca
    27601 tttagacgga cgagatgtct taatcatgtc tccgatacaa atcaagaaga acggactgga
    27661 atatcataat atcagttcta ccatggcttg tattggtgag gtaaactggg aaacaggtca
    27721 gatgatagtt gataatttcc atgaaatgga ttttggaatg gatttttatg caccacaaac
    27781 aacaagagat cctaatggtg gacgggtgat gattgcttgg atgcagatgt gggatcgtac
    27841 attgcctaca catgagttag gccatctttg ggcaggctct atgagtttgc cccgagagtt
    27901 aagagtgaaa aaaaatcgat tggtacaaaa acccatctca actgtatata gtcatttagt
    27961 ttatcaacaa ggcagtgaaa atattgcagt gactgacaca tcagtcgtat ttcgaaacgc
    28021 gctgtctgat tcttcatatt tacatctagt cgcagatttg gagaaagctg aagtttttac
    28081 aattgaatta ctgcggtcta agacggattg tctcaaacta gcctatgaca ctgctacaca
    28141 ggtttttgaa ttggatcgtc agaaactagg aagagagctt acaggggcgg aagaagaccc
    28201 tctgaatgta cgaaaagttg aagtaccttt gattgataat cagctcgttc ttgagatttt
    28261 tagagatacc tcatcaattg aagtctttat taatgggtta gaagttatga cggctacttt
    28321 ctatgagctg gaaaaagctc gagatatctt gtttcattcg atcggagaaa gtcgtattca
    28381 agcttttgaa acagcgagtg tgaaataaat gtttgattgc ttaggcgatg aatcaattat
    28441 ttaataggtt ttgaaggata atgtttttag aaaagaacag atagctagat ttcccatgga
    28501 tttgctatta gtattttgaa aaaattagta atttatgaca aaattcatta tactaaactt
    28561 gtgcagaaat gtgagggatc aactatgaaa gcaacaatga aagatgtcgc aaaattagca
    28621 ggtgttggag taggaactgt ttcccgggta ttgaataacg gttctgttaa agcttctact
    28681 agagaaaaag ttgaagcagc aatgacagag ttaaactatc aaccagattt ttatgcccgg
    28741 gggttaaaga tgaagcatac gaacacggtt gctttgattc tacccacggt atggcatcca
    28801 ttttttggcg aatttgccta ccatgtagaa acagcattag aaagacaaaa ctacaagctt
    28861 tatttgtgta actccagtgg taatagtgaa aaagaacaag aatatatcca gatggtcagc
    28921 caaaataaag tagacggcat cattggaatt acttattcag atatcgatga atacatttcg
    28981 agtaatctgc cttttgttag tattgaccgt tatttcactg aagaggtgat ttatgtgacc
    29041 gctgacaatg cggccggtgg acgtttggct gcaaatgaac tttatcgccg aggatgtcag
    29101 aatgtagcat atatcggtgg gtaccaagaa acaccgaatg aaacgaagag tcgtcgaaaa
    29161 tattttgaaa tcgagtgccg tcaactgggg atttcctata aagcgttaga tatgttagaa
    29221 cctttagacc atctctcaga acgtgtaaca aattttttgt tagagaatcc cgatatcgat
    29281 gggattttta cgatcaacga tccaatggca ttagctgtca ttgatatgct tgaaaaaata
    29341 gggaaaaacg taccagagga tgtccaagtt atcggatttg atggtcaaaa gatgacagaa
    29401 ggagatcctt atctagtctc aacgattgcc caacctacaa agcaaatggc ggaagtggca
    29461 gtggcaaaat taattgctgt cattaatgga gagacagtag aaaaacgtac cgtattgccg
    29521 attgaatttg gcgaaggtga cacaacgaaa cctttagtga gaaacgattt tgcttagtgc
    29581 aaatacgttt ctttttttga aaatgagtga atgaaaggtg tagataaaat gttatatggt
    29641 ggaatagaag ccggtggaac aaagtttgtc tgtgggattg gtaatgaaca attacaagtg
    29701 atagaacgga aaagttttcc taccacaaca ccagaagaaa cgatggagca agtgtttgat
    29761 ttctttgata agtacaaagg aaatatcgca tcaattggtg taggagcatt tgggcctatt
    29821 gatattcaaa tcgaatcaag aacttacgga tatatcacca acacaccgaa gttagcttgg
    29881 caaaattatc attttgtagg agctttggaa aacgccttgt cggttcccat ctattggact
    29941 accgatgtaa atgctgcttg ttatggagaa tatatggcag ggtatggaaa aggtaaaaat
    30001 agtgttctgt attacaccgt tggtacaggt gttggtggtg gtgctgtagt ggatgggaaa
    30061 atagtcgcag gttttagtca tcctgaaatg ggacatatgc tcttacaacg tcataatgaa
    30121 gatcaatttg aaggaatctg tccttttcat catgattgtt tggaagggtt agcatcagga
    30181 cctgcgattg aaaaacgctt tggaagaaaa gcccagacta tctcatttga tcatccttat
    30241 tggacgattg aggcagatta tcttgctcag tgtgtgtata acacaacgtt actatatgca
    30301 ccggaagtaa taattttagg cgggggtgta atgaaacagc cgcaactact cgagttgatt
    30361 cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata cgcctactct cgaagaatat
    30421 ctactaactc caaggcttgc tgacaatgcg ggcttgattg gctgcctagc tttagcaata
    30481 aagaaactca atcattagaa tagtatttat tagaactatt cataacatac cggaagttta
    30541 aaagggtatt ctgaaactta aatagtctca attcattctt ctttcttgag aatctgagta
    30601 gagactttta ttctgtttca aattaatata attacgaaat gatattgagt acaagaaagt
    30661 tctttttacc agatctttta aatcgaaaaa tctgatatat atatttatag tactaaaaat
    30721 ctgattagat aaaaagctaa ttcaatagga aaataaaggt agtgaataaa agcaaagccc
    30781 ttgttagtag ttaatctaac aagggctttg gtagggaaaa tggtaggaaa ccatcttatt
    30841 ttttacgaaa atcaggaact ttagtaattg aataatgttg atttataggt attcctcatc
    30901 catattatgt tggctcttta ttcccactcc actgttgcag gtggtttgct ggtaatatca
    30961 tacacgatac ggttaacgtg tgcgacttca tttacgatac ggacactgat tttttgcaag
    31021 acttcccaag ggatacgggc aaagtcagcg gtcatgccgt caatagaagt gacagcacgg
    31081 atacctacag tgtagtcata cgtacggcca tctcccataa ccccaacaga gcgaatgcct
    31141 ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac cggcattggc gatttcttcg
    31201 cgcaagatag cgtctgattc acggacgatt tctaattttt cttctgtcaa ttcaccaagg
    31261 acacgaatac ctaagcctgg tccagggaat ggttggcgcc aaacgatgct gtcaggcatg
    31321 cctaattggg tacctaattc acgtacttcg tctttgaaca atgtgttcaa tggctcgatc
    31381 agcttgaatt gcatgtcttc aggtaatcca cctacattgt gatgtgattt gatggtttgg
    31441 gcagtttcag ttcctgattc gatcacatcg gtataaaggg ttccttgtgc aaggaaagaa
    31501 acgccttctt caccggcaag tttggttgct tcatcatcga agacatatac aaattcattt
    31561 ccaatgattt tacgtttttc ttctggatca gacacgccag caagttttga taagaaacgt
    31621 tctttggcat cgacttttat aatgttcaag ccaaattttc cgccaagact ttccataact
    31681 tgttctgctt cacctttacg caacaatacg tgatcaacga agatacatgt taattgatcg
    31741 ccgatcgctt tttgtaaaag aacaccaaca acagaggaat ctactccgcc tgaaaggcct
    31801 aataaaactt ttttgtcacc gacttgttca cgaatttttg cgatctccat gtcgatgaag
    31861 ttatccattg tccagtcacc agtacattgg cagacatcta gtgtaaaatg gcgcaacaag
    31921 tcatttccat attcggaatg gcgaacttct gcatggaatt ggattccgta cattttgcgt
    31981 tctgcatttt ggatcgatgc gatggggcaa tctttgctgg tagcaacgac atcgaaatca
    32041 gtcggtgctt gggtaactag gtcaccatga ctcatccaaa cggtttgttt ttcaggagtg
    32101 ttttggaaaa gggccgcttc tgcagaagtg atttccaatt ctgcttttcc gtattcacgg
    32161 tttttggctg gtttaacttt tccgcctaat ttataagtaa tcaactgcat accgtaacag
    32221 attcctaaaa ctgggatccc caaatcaaaa atcttttcat caataccaaa ggccttctca
    32281 tcataaacac tgtttggtcc gccagaaagg atgattcctt tagggttcat tgcctttact
    32341 tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa caccgaactc acgaatacgg
    32401 cgagtgatca gttggttata ctgactaccg taatcaagta cgataatttt tggctcttcg
    32461 tcaaatcgtg ttggtgaaga caaatttgaa taaaacttag tccgatatgc ggcgcgttct
    32521 gtgcgccgca tattttttta gttttcaaag atcagacctt gttgtaaata gatccgtgcc
    32581 cgaaagttat aaaagttgcg ataaccaaac gcaattcgtt taatcagctt gatcttgtta
    32641 ttcagcccct cagtgacgcc gtttgagtgg gacaccttaa aggcatttga aatacccgat
    32701 tggtagcgat ggaagatcgt gaatttcttt ttaaacgact gaggtaacgt agatgaaatt
    32761 gttttaaggg tttcttgaaa gccttccgta
//
LOCUS       DASJSZ010000026        32220 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.026, whole genome shotgun sequence.
ACCESSION   DASJSZ010000026 DASJSZ010000000
VERSION     DASJSZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.026"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            264..452
                     /locus_tag="U4B03_001125"
                     /pseudo
     CDS             264..452
                     /locus_tag="U4B03_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            498..1463
                     /locus_tag="U4B03_001126"
     CDS             498..1463
                     /locus_tag="U4B03_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2067995.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            1485..1697
                     /locus_tag="U4B03_001127"
     CDS             1485..1697
                     /locus_tag="U4B03_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN2067996.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            1772..2248
                     /locus_tag="U4B03_001128"
     CDS             1772..2248
                     /locus_tag="U4B03_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEN2067997.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            2245..4053
                     /locus_tag="U4B03_001129"
     CDS             2245..4053
                     /locus_tag="U4B03_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEN2067998.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            4130..4345
                     /locus_tag="U4B03_001130"
     CDS             4130..4345
                     /locus_tag="U4B03_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HEN2067999.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            4437..5300
                     /locus_tag="U4B03_001131"
     CDS             4437..5300
                     /locus_tag="U4B03_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068000.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            5337..5873
                     /locus_tag="U4B03_001132"
     CDS             5337..5873
                     /locus_tag="U4B03_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEN2068001.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            5887..6309
                     /locus_tag="U4B03_001133"
     CDS             5887..6309
                     /locus_tag="U4B03_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEN2068002.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            6239..8638
                     /locus_tag="U4B03_001134"
     CDS             6239..8638
                     /locus_tag="U4B03_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEN2068003.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            8670..10661
                     /locus_tag="U4B03_001135"
     CDS             8670..10661
                     /locus_tag="U4B03_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN2068004.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            10684..10935
                     /locus_tag="U4B03_001136"
     CDS             10684..10935
                     /locus_tag="U4B03_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HEN2068005.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            10925..12154
                     /locus_tag="U4B03_001137"
     CDS             10925..12154
                     /locus_tag="U4B03_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HEN2068006.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            12151..14232
                     /locus_tag="U4B03_001138"
     CDS             12151..14232
                     /locus_tag="U4B03_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEN2068007.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            14381..18301
                     /locus_tag="U4B03_001139"
     CDS             14381..18301
                     /locus_tag="U4B03_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HEN2068008.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            18302..19246
                     /locus_tag="U4B03_001140"
     CDS             18302..19246
                     /locus_tag="U4B03_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068009.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            19250..19453
                     /locus_tag="U4B03_001141"
     CDS             19250..19453
                     /locus_tag="U4B03_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HEN2068010.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(19753..19989)
                     /locus_tag="U4B03_001142"
     CDS             complement(19753..19989)
                     /locus_tag="U4B03_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2068011.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(20172..20558)
                     /locus_tag="U4B03_001143"
     CDS             complement(20172..20558)
                     /locus_tag="U4B03_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HEN2068012.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(20540..21868)
                     /locus_tag="U4B03_001144"
     CDS             complement(20540..21868)
                     /locus_tag="U4B03_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN2068013.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(21829..22158)
                     /gene="mobC"
                     /locus_tag="U4B03_001145"
     CDS             complement(21829..22158)
                     /gene="mobC"
                     /locus_tag="U4B03_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEN2068014.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(22416..22786)
                     /locus_tag="U4B03_001146"
                     /pseudo
     CDS             complement(22416..22786)
                     /locus_tag="U4B03_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            22931..23116
                     /locus_tag="U4B03_001147"
     CDS             22931..23116
                     /locus_tag="U4B03_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2068015.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            23274..23441
                     /locus_tag="U4B03_001148"
     CDS             23274..23441
                     /locus_tag="U4B03_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068016.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            24059..24718
                     /gene="vanR"
                     /locus_tag="U4B03_001149"
     CDS             24059..24718
                     /gene="vanR"
                     /locus_tag="U4B03_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HEN2068017.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            24721..26082
                     /gene="vanS"
                     /locus_tag="U4B03_001150"
     CDS             24721..26082
                     /gene="vanS"
                     /locus_tag="U4B03_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN2068018.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(26173..27474)
                     /locus_tag="U4B03_001151"
     CDS             complement(26173..27474)
                     /locus_tag="U4B03_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2068019.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            27753..28559
                     /locus_tag="U4B03_001152"
     CDS             27753..28559
                     /locus_tag="U4B03_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HEN2068020.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            28577..29284
                     /locus_tag="U4B03_001153"
     CDS             28577..29284
                     /locus_tag="U4B03_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEN2068021.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            29281..30252
                     /locus_tag="U4B03_001154"
     CDS             29281..30252
                     /locus_tag="U4B03_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HEN2068022.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            30245..31273
                     /gene="vanB"
                     /locus_tag="U4B03_001155"
     CDS             30245..31273
                     /gene="vanB"
                     /locus_tag="U4B03_001155"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HEN2068023.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            31279..31887
                     /locus_tag="U4B03_001156"
     CDS             31279..31887
                     /locus_tag="U4B03_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HEN2068024.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(31953..32126)
                     /locus_tag="U4B03_001157"
     CDS             complement(31953..32126)
                     /locus_tag="U4B03_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068025.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         8167 a         8197 c         8689 g         7167 t
ORIGIN      
        1 aatattatag aaaaaatttt aggttatcgc tgaaagcccc ggaaatacgg gcatttcgca
       61 ggatatgaga actcaattta ctaccaattt actactttcg gattgagccg catatatgct
      121 gaaccatact gctaataccc gccaccccaa atacggcaca tcataaattg aatacgacac
      181 cgtaaacgcg gcgttcctct ttcccgtcat gggagaacag gacgccgctt ttttatgccc
      241 aaatagagaa aggaggcgac gccatgctat ttacttccgg ccctgaccgg gcgtttgaac
      301 ggctgatgca ggaaaagccc ggtttcgatc actacgacgg cggcggggcg gacgcgccgg
      361 aggattgcgg cacctgccga ttttaccgcc cggattggaa atatcaattc tgccagtttg
      421 cagagtgtcc ctatcagccg ggcaagctga ccgactatgc agcggtcaaa ttcacagtga
      481 aaggagcgaa caacgctatg gcagttttcc gtgtggaaaa gaacaaaggc tatacggtga
      541 tgtcaaatca ccacctgcgg aataagaacc tgaccttaaa ggccaagggc ctgctgtcgc
      601 agatgttgtc cttgccggag gattgggact ataccctaaa aggactgtcc tatatcaacc
      661 gggaaagcat cgacgcgatc aggacagcgg tatgggagct tgaaaaagct ggatatatcc
      721 ggcgtgagca gggccgtgac gcaaaaggca aaatggccga tatggtctat accatctacg
      781 aacagccggt attggaaaac ccggtattgg aaaatccaac atcggataac ccggtgttgg
      841 aaaatccgac atcggataat ccggcgtcgg aaaatccaac gcaattaaat aaagaagtac
      901 aaagaactga cttatcaaaa aaagaaaaaa taattacaga tggacaaagt acccattcca
      961 ttcctatccc atccccgacc cctgccccta tggagggcga ggcagcggct acgccgccgg
     1021 aacggaaaag gacaggaaag gatgaggctg tacggattta tcgggaaatc attttggaga
     1081 acatcgagta tgcctatctc attcaggaca ggagcattga ccgtgaacag cttgacgaaa
     1141 tcgtagacct gatgctggaa accgtctgca ccgcccgaaa gacaatccgt gtcgccgggg
     1201 acgactaccc tgccgagctg gtgaaatcca agtacatgaa gctgaccggc gaacatatcc
     1261 gctttgtcct tgactgtctg cgggagaaca caacgaaagt ccgcaatatc aaacaatacc
     1321 tgcgggctat gctgttcaac gcccccagca ccatttccaa ctactacacg gcccttgtcg
     1381 cgcacgacat ggcacagccc gattggggaa agcctaaatc cggcctgccg gactattcct
     1441 gcggcgaggg cgaaagcctg taacgagaaa aggaggacga ccctatgaag cagggcgcat
     1501 tgatttttga cgagcgaaca gaccgttacg acatccgctt tgatctggcc gactattacg
     1561 gcggcctgca ttgcggggag tgtttcgacg tgatgatcgg cggcaaatgg aggcccaccc
     1621 gcattgaaat ggccgaaaac tggtatcttg tcggcatccg ggccgacgac ctttccggcc
     1681 tgcgggtgcg gatttgacct atatctacct gcggcgtctg gtggttccgc cttagacgcc
     1741 gcttttgact ttctgacaag gagggatgcg attgcaggaa gaagttactc aaaagaccat
     1801 tgccctgtcc atgaaaacgg gcaagctcac ggctcaagtg ctgcaagcgg cgctgaaaaa
     1861 gtacctgcaa caccgggcca agggcaaaaa cacgctgcac cacggccagc agagcctaaa
     1921 gcagcttaaa aagcatgggg ctgatctctc caacattgag atcaccgagg ccaacattgg
     1981 agcgttcaag ccatgcgcca agaaatacgg cgtggatttt accttacgaa aagataagac
     2041 cacccagccg ccccactacg ttgtgatttt caaggccaag gatgcagata accttgatct
     2101 ggcgtttcgg gagtttaccg ccaagacgct ttccaaggag cagcggccct ctatccgcaa
     2161 ggttttgtcc gctgtgaagc aaaaggcggc ggcccagacc aagcagcggg ccaaggagaa
     2221 aatcaaggaa agggggctgg aactgtgaag cctgacgtaa agaaattgct gatcctgaac
     2281 ctcccgtatc tgatttttgt gtacctgttt gcgaaatgcg gggaggcgta ccggctggcc
     2341 gctggtgctg acctgtcggg gaagctcctg cattttgcgg atggttttgc ggcggctttt
     2401 gccaaccctc tgccaagcct gcacctgttt gacctgtgta tcggtatcgc cggtgccgtg
     2461 atcgtgcggc tggtggtgta ctgcaagagc aagaacgtga agaaataccg caaaggcatg
     2521 gagtatggca gcgcccgctg ggggacggcc aaagacatag cgccttacat cgaccccaag
     2581 tttgaaaata atatcctgct cacccaaacg gaacggctta ccatgaccgg caggcccaaa
     2641 gaccccaaga cagcgcggaa taaaaacgtg ctggtgatcg gtggcagcgg ttcagggaaa
     2701 acgagatttt tcgtgaaacc caacctgatg caatgctttc cgaccacgga ctatccgact
     2761 tcgtttgtgg tgacagatcc gaaaggcact ttggttctgg aaaccggcag gatgttccag
     2821 caggcgggct accgcattaa aatcctgaat accattaact tttccaagtc catgaagtac
     2881 aacccctttg tctacatcca ttcggaaaag gacgtgctaa agctggtaaa taccctgatt
     2941 tccaacacca agggcgaggg ggaaaagtca gcggaggatt tttgggtgaa gtcggaacgg
     3001 ttgttttatt cggccctgat cggctatatc tggtacgagg ccccagcgga ggaaatgaac
     3061 ttctcaacgc tgctggaaat gataaacgcc agtgaagccc gcgaggacga cccggagttt
     3121 caaagccccg tcgatctcat gtttgagcgt ttggagcaga aagaccccga ccattttgcg
     3181 gtgcgccagt acaagaaatt cctgctgtcc gcaggcaaga cccgttcttc catcctgatt
     3241 tcatgcggtg ccagacttgc gccctttgac atccgggagg tacgcgagct gatggaggac
     3301 gacgaaatgg agctggacac catcggggac gaaaagacgg tgctgtatct cattatgagc
     3361 gatacggaca cgacctttaa tttcattctg gctatgctcc aaagccagct aatcaacctg
     3421 ctttgtgacc gggctgatga tgtgtacggc gggcggctgc ctgtccatgt acgcctgatt
     3481 ttggatgaat ttgccaacat tggccaaata cccaatttcg ataaactcat tgccaccatt
     3541 cgaagccgtg aaatatcggc ctctattatt ttgcagagcc agtcgcagct aaaagcgatt
     3601 tacaaggacg ccgccgagat catttcggat aactgcgata gtgtgctttt ccttagtggg
     3661 cgcggcaaaa atgccaagga gatttccgat gcgctgggga aagaaacgat tgacagtttc
     3721 aacaccagcg agaaccgggg aagccaaacg tcccacggat tgaactatca aaaattagga
     3781 aaggagttga tgtcccaaga cgaaattgcc gtgatggacg gcggcaagtg tatcttgcag
     3841 ctacggggtg tgcgcccgtt tttatcggag aaattcgata ttaccaagca tccccggtac
     3901 aaataccttg ccgacgccga caagaagaat acctttgacg tggacagatt tttgactatc
     3961 acgcgccgga aacggcagca agtggtaacg caggatgaag tattcgacct gtatgaaatc
     4021 gacctgtcgg acgaggacgc cgccgctgaa taaacagcgg cttttttcat tccaacaaca
     4081 aaatcaaaaa atcattatga gccggaaacc ggcagaaaga ggtaaattta tggcattttt
     4141 taattccgca gttactactt tgcagactat cgtgatcgga ctgggcggcg ctctgtgcgt
     4201 atggggcggc atcaatctgt tggagggata cgggcaggac aatccggggg caaaatcgca
     4261 gggtgtcaag cagcttgtcg ctggcggcgg cgtggccctg atcggtgtca ctctggttcc
     4321 cctgctgtcc gggctgctgg gctgatcctg gcctttgtcc ctgtccaaac ccttaactaa
     4381 tcgcctccgc gtcctcccgg ccagaccggg agggcgcggg aaaggaggtg ctacccgtga
     4441 ttagtgaaat cattgaagaa tggataaaag gcatcctcat ggacggtatc acgggcaacc
     4501 tgtccgggct gttcgatacg gtaaatgcca aagtcggaga aatcgccgcc gacgtgggac
     4561 gaaccccgca ggcatggaac agcggtattt tcaatatgct gcacagcctg tcggaaaccg
     4621 tgatcgtccc catagccggg gccatactcg cccttgtcat gtgctatgag cttatccaga
     4681 tgataacgga aaagaacaat atgcacgact ttgacagctc catgttcttc cgctggatat
     4741 tcaaatccgc gtttgcaatc ctgatcgtaa cgaacacatg gaacatcgtc atgggcgtat
     4801 tcgacgccac ccaaagcgtc gtcaatcaaa gcgccggtgt gattatcggg cagacatcca
     4861 ttgattttaa caccctgata ccagaccttg aaagccaatt ggaggctatg agcgtgggcg
     4921 ctcttttggg gctgtggttc caaacccttg ttgtgggcct caccatgaat atcctttcca
     4981 tctgcatttt ccttgtgacc tatggccgca tggtggaaat ctatgttgtc acgtcgctgg
     5041 ggcctatccc ccttgccaca atgggcaacg cggaatggcg cggcatgggc caagggtatc
     5101 tgaaatccct gctggcgctg ggcttccaag cctttttaat catggttgtg gtgggtatct
     5161 acgcggtgct gatacagaat atcggttccg cttccgacat atccggcgca atctggggct
     5221 gtatgggcta tacggtgctg ctgtgcttct gcctgttcaa gaccggcagt atatcaaagg
     5281 ccgtattcgg ggcgcattga tcccaaaatc caagcataca caggaggaac ttgtttattt
     5341 caacgtacaa catcatttac gctgacccgc cgtggcggta tgactgcaaa aacgtacagg
     5401 gcgcagcgga aaatcactat tccaccatga gcattgacga gctgtgcgcc ctgccggtgg
     5461 aacggctggc gtccaaagac tgcctgctgt ttttgtgggc cacattcccc atgctgccgg
     5521 aagctctgcg gttaatcaaa gcgtggggct tttcattcaa gaccgtggct tttgtatggc
     5581 tgaaacagaa ccgtaaaagc cttacatggt tttacgggct gggccgctgg acaaggggaa
     5641 acgcggaaat ctgcctgctg gcaacacacg gcaagccgaa gcgtcgttct gcctctgtcc
     5701 atcagtttat tatcagcccg attgaacaac acagcaaaaa gccggatgtg acccgtgaaa
     5761 aaatcgtaga gctggcgggc gatttgcccc gcgtggagct gtttgccaga cagaaaaccc
     5821 ccggctggga cgtgtggggg aacgaggtgg aatgtgacct tatcctgacg tgaaaggagg
     5881 tgccaaatgg cttatgtaac cgtccccaag gacttaaccc atgtgaaaag caaggttgtg
     5941 tttgggctta ccaaacggca actgatctgt ttttccgggg cgctggtgat cggcgtcccc
     6001 ttatattttc tcacccgtga ctatcttacc aacagcgcgg cggccctgct catggtattt
     6061 gccatgctgc cgggcttttt gcttgccatg tacgagcgtc acggccagcc ccttgaagtg
     6121 gtgattatgc agatcatcca gtgctgcttc atccagccaa aggagcgtcc ctatcagacc
     6181 gacaacacct acaccgccct tgtgcggcaa taccagatgg aacaggaggt aaaggccatt
     6241 gtccaaaaag gaaatcccaa gagaaaccga aaaaacaaag ctgacccgcg cccagcgaaa
     6301 agaaattgac gcggtaatcc gtaaatataa gggcgacggc aggccccaca cggcgcagca
     6361 gagcatcccc tacgaggtca tgtacccgga cggcgtgtgc cgggtatcgc ccggcgtctt
     6421 ttccaagtgc attgagtttg cggacatcag ctatcagctt gcccagccgg acacgcagac
     6481 ggctatcttt gaaaaactgt gcgacctgta taactatgtg gacgcctcca ttcatatcca
     6541 gttttctttc ctcaatcgga aagttgaccc ggtgcagtac gccaagagct ttgagatcgc
     6601 gccgcagggg gacgacttcg acgacatccg ggccgagtac accggcattc tgcaaaagca
     6661 gcttgcaaac ggcaacaacg gcatggtaaa gaccaaatac ctgacattca ccatcgaggc
     6721 cgaaagcgtg aaagcggcgc gagccagatt gaagcgcatc ggctttgacc tgttaggcta
     6781 tttcaagagc atgggggccg tagcccatgt gatggacggc tgggaacggc tgaacctgct
     6841 gcatggcgtg taccacccgg acggcgagat attcaatttt gactggaagt ggctggcccc
     6901 gtccggcctc tccaccaagg attttattgc cccgtcctcc ctgtgcttcg gcaatgcaaa
     6961 gacattcggc atgggcggca aatacggcgc ggtaagtttc ctgcaaatcc tgtccccgga
     7021 gctgtcggac gatatgctgg ccgacttcct caatacggaa agcggtgttt tagtcaatct
     7081 ccatgtgcag gccattgaac agaccaaggc gatcaagacc atcaagcgca aaattaccga
     7141 ccttgacgct atgaagattg cggagcagaa aaaggccgtc cgcagcggtt acgatatgga
     7201 cattcttccc agcgaccttg ccacctacgg cgaggacgcc aagaagctgc tgacaaagtt
     7261 gcagacccgc aatgaacggc tgttccagtt gacgttcctt gtcctcaacg tggccgacac
     7321 caagcaaaag ctgaataacg acgtgttcca agccgcaggc gtggcccaaa agcataactg
     7381 tccccttgtc cggctggact accagcagga acagggcctt gcaagcagcc tgccgctggg
     7441 cgtcaatcag atcaagatac agcgcagcct tacgacttcc agcgtggccg tgttcgtccc
     7501 ctttgtgacg caggagcttt ttcagggcgg cgcggccatg tattacggca tcaacgccaa
     7561 gtcccgcaac atgattatgc tggacaggaa gcaggcccgt tgccccaatg ccttgaagct
     7621 cggcaccccc ggcagcggca aatccatgag ctgtaaatcc gagatcgtca gtgtgttctt
     7681 aacgacgcct gacgatattt ttatttcaga cccggaggcc gaatattacc cccttgtcaa
     7741 acggctccac ggacaggtaa tccggctgtc gcctaccagc aaggactttg tgaacccgct
     7801 ggacatcaac ctgaattatt cggaggacga taacccgctg gccctgaaat ccgactttgt
     7861 gctgtcgttc tgtgagcttg tcatgggcgg caagaacggg ctggaagcca ttgaaaagac
     7921 cgtgatagac cgggctgtgc gcgtgatcta ccggccctat ctggcagacc cccgcccgga
     7981 gaatatgccg atcctgtccg acctccacaa agcgttgtta gatcagcatg tcccggaggc
     8041 tgaccgggtg gcgcaggccc ttgacctgta tgtgtccggc tccctgaacg tatttaacca
     8101 ccgtaccaac gtggacatcg gcaaccggct tgtgtccttt gacattaagg agctgggcaa
     8161 gcagctcaaa aagctgggga tgctcattgt ccaagaccag atatgggggc gcgtcacggc
     8221 caaccgaagc caaggaaagg ccacatggta ttttgccgac gagttccatt tgcttttgaa
     8281 agaggaacag acggcggcct attctgcgga gatttggaag cggtttagga aatggggcgg
     8341 cattccaaca ggtgccaccc agaacgtcaa agacctgctt tcctccccgg aaattgaaaa
     8401 tatccttgaa aacagcgact tcattacgct gctcaatcag gcgtccgggg atagaaaaat
     8461 ccttgcggaa cgcttgaatt tgtccaccga acagcagaag tacatcgaca attccgaacc
     8521 cggcgagggc cttttgatct ttgaaaacgt ggtgctgccc tttaccaacc ccatcccgca
     8581 caacacccag ctttacaaga ttatgacgac ccgcttaaac gaggtggcgg gagtatgacc
     8641 cggcgacgcc acaaaggagg tgaaccacca tgaaagagct gaaagccaag gcgaaagtga
     8701 cgcaggccat gacccgcgac gggctgacgc tggaaaatca ggccaccggc gaggtggaga
     8761 acatttccag ccgggaggcc gaacaggatt tgtccgcgcc ggggccgggc ggcaccgccg
     8821 ataagctgct ggaacgggcc gacgccatcc atgacggcca taaggccaag aaagccgcga
     8881 agcaggccgg ggctgtggcc gccgaggggg acgctgccct gcaccggcca tccgcccggc
     8941 tccaactgtc ggaggaagaa cgggccgacc cggatttaca gccctatatc cgaaaatcgg
     9001 acaaacgggc cgataagctg gataaggccc gcgccgccat ccccaagaag cgcgtcccgg
     9061 tcaaggaaac cgtctttgac gaggcaagcg gcaaagcaaa gaccgtcctg cactttgaca
     9121 aacaggacaa ggggcctccc tccctgaaac ccaatccggc aggccgtcct gtttccgagg
     9181 tttttctatt cggccacggg aaaatccatg aagtcgagca tgaaaacgtg ggtgtggagg
     9241 gcggccataa gggggaggaa cttgccgagc ggcaggccgg aaaagccatc cgcagcacca
     9301 tccggcacca caggctgaaa ccctactggg acgcggaaaa ggccgagcgc aggctattgt
     9361 ctgccaacgc ggagtatttc taccagaaat ctttgcggga taacccggag atcgcacagg
     9421 cggccagcaa ccccatttcc cggttctggc agaaacagca gatcaagcgc cagtacgcca
     9481 aggccgcccg gacagcgggc cagaccgcag gcgcggcggc acagggcgcg gccaagacag
     9541 ccggggcctc cacgaaagcg gccaaggccg cagccaaaga aagtgaacgg gcggcctcct
     9601 ttgtggcccg ccactggaaa ggggcgctgg ccgtccttgt gttggggctt ttgatgctgc
     9661 ttatcatcgg cggcttacag tcctgcaccg ccatgttcgg cagcgccgga agcggcatca
     9721 cggcgtcctc ttacttttcc gaggatagcg atatgttggc cgccgaggac gcctacgcgg
     9781 agctggaagc cgagctgcaa gactatctgg acaactacga ggccacccat gactatgacg
     9841 aataccattt tgaccttgac gagatcagcc atgaccccta tgtgctgatt tccattctgt
     9901 cggcgctcca tgacgggata tttaccatcg gggaggtaca gggtgatttg tccatgctct
     9961 ttgacaagca atatatcctg acagaaaacg tcgtggtaga aacccgttac cgcaccgaaa
    10021 ccgatacatg gacggacgcg gagggcaaca cccatacaga cacctaccaa gtgccgtatg
    10081 actattacat ctgctacgtc acgctggaaa acttcgacct gtcccatgtg ccagtctata
    10141 tcatggacga ggaaacgcta agtttctatt ccctgtatat gtccacgcta ggaaaccggc
    10201 ctgatctgtt ttccggcagc cagtacccca acgcctccac cctgacgccg ccgacctact
    10261 acgacgtacc cccggaggct ttggaggatg ccacctttgc cgccatgctc aaagaggcgg
    10321 aaaaatatat cggttatccc tatgtgtggg gcggcagctc tcccagcacc agctttgatt
    10381 gcagcggctt catttcgtgg gtaatcaatc atagtggatg gaacgtggga cggctggggg
    10441 cgcagggcct atataacatc tgcacccctg tttccagttc gcaggccaag cccggcgacc
    10501 ttgtattttt caagggaacc tacgacaccc ccggcgtgtc ccacgtcggc ctgtatgtgg
    10561 gcaacaatgt gatgctgcat tgcgggaacc cgatttccta cacgaacctt acttcaaact
    10621 actggcaatc ccatttttac agttacgggc gtttacctta acgccagaaa ggagctttca
    10681 attatggcaa ccaaacttga ccgcatcgaa aaggacatcc agaaaaccaa gtctaagatc
    10741 gcggagtttc agaaacagct tcgggagctg gaaacgcaga aaaccgaaca ggagaatttg
    10801 cagatcatcc agcttgtgcg cggcatgaac atgaagccgg aggaatttgc cgcgttctta
    10861 cgcagcggcg caatgcaggc cgcccctgcc gcaacccctt accatgaaca ggaggacaat
    10921 gcccatgaag aataagacca gacggatttc ccgtattctc cccgcacttc tggccgtgat
    10981 gctctgcgtc acggcctttc ccgttaccgc ccttgcaggc ggcaatgacc cggcaccggc
    11041 cccgctgccg gaggctacaa ccgaacccgc tacgggcggt ttggagcctg aaacagacga
    11101 aaccggcacc gccacggacg ggacagaaac cgaggccgaa aaagataccg gccctctgac
    11161 cggcaaggac atcaccgacc tgttttccgc gctgttcggt tccaaggtga gcattgccgc
    11221 cacggacgac ggcatccaga tcaccaccgg caccgacaaa aaagaacctg aacagaccgg
    11281 caccgtcacc accaacggcg gcaggctgaa tgtccgcacc ggcgcggggc ttgacaagac
    11341 cgcgttcacc cagcttccca atgggacgac ggttgaggta gtcggcacag acggcgactg
    11401 gattaaaatt ctcttgccgg agcgcatcgg ctatgtccat tccgactata tgaccgtcag
    11461 cgaaaaggaa gtagcggcta ccggcgaggg cggtttttcc ctctccattg acccggagga
    11521 aattgcttct ctgctggaac tgttcaatgg cggcggggcc agcgccgccc tgtccccgga
    11581 cgggaacctg tcactgattg acgacatcgg cagcgccgcg aaaaatggca agcagtttat
    11641 caccgtggaa accaaaaacg gcaatgtgtt ttatctgatt attgaccggg acgacgaggg
    11701 aaaagaaact gtccatttct taaatcaggt ggatgaagcc gatctgctga cgctgatgga
    11761 ggacggggaa accgtcaccc ccgctgctgt ctgctcctgt accacgaaat gcaaggccgg
    11821 ggctgtgaac acgaattgcc cggtatgtaa aaccaacctg accgagtgca gcggcccgga
    11881 gccgcaggaa ccccagccgg aggaacccga agccccggag gaagaaccca agggcggcgc
    11941 gggcggcctg atcgtgttcc tgctggtggc gctggcgggc ggcggtgcgg ccctttacta
    12001 tttcaagttc aaaaagccca aggctgaaac caccggcagc gacgatttgg gcgagtatga
    12061 ttttggggag gacgaggatt tggaggacga ggaacccgaa accgagtttg acccggaagc
    12121 tgattttgtg agtgaacagg aggacgagga atgacatata aacttgtaat cgcggaaaaa
    12181 cccagcgtgg cccgcagcat tgcgggcgtg atcggggcca cggagaaaca cgacggctat
    12241 ttggagggga acggctatct ggtgagctgg tgcatcggac atctggtgtc ctttgccgac
    12301 gccggacgct atgacgagcg tttcaaaaaa tggcgctatg aggatttgcc cattctcccg
    12361 gaaacatggc agtacatcgt ccccgacgac aagaaacagc agtttgacgt tctctgttcg
    12421 ctgatgcagc gccccgacgt gaccggcctt gtgtgcgcca ccgacgccgg gcgcgagggg
    12481 gaattgattt tccgtttcgt ctaccagatg gcgggatgcc aaaagccctt tgagcgcctt
    12541 tggatttcct ctatggagga cagtgctatt aaggacggct ttgcccacct gaaaccgggc
    12601 ggcgactatg atccgctgta tcaatcggcc ctgtgccggg ccaaggccga ctggctgatc
    12661 ggcattaacg ccacccggct tttttccgtc ctgtaccaca agaacctgac tgtgggccgc
    12721 gtccagacgc cgaccttaaa aatgctggtg gacagggacg ccaagattac cgatttcaag
    12781 aaagagaaat atcacatcgt ccatatcacg gcgggcggcg cggacgcggt aagcagccgc
    12841 ttttccgacg ctgccgaggc aaacaccgtc aaggcggctt gtgcaaaagc gcaggccgtt
    12901 tgcgtttccg tgacgcggga gaaaaagacg gaacagccgc ccaagctcta tgaccttacc
    12961 accttgcagc gggaggctaa ccggcttttc ggctataccg ccaagcagac ccttgactat
    13021 gcccaggcac tgtatctaaa gaagctgctg acctatccga gaacggatag tcaatatctt
    13081 accgacgata tgctgcccgc cgtggaaagc cttgtgtccg gcctgtggga gctggtgccg
    13141 tttgccaagg gcctgaacct ttccccgcag tttgaccgca tcctgaacag caaaaaggtg
    13201 tccgaccacc acgccattat ccccaccgct gaatttgtga agcagggctt tggcactctg
    13261 gccgagagtg aaaaaaagct gctgtccctg atctgctgca agctcttatg tgccgtggcc
    13321 gggccgcata tctatgaggc cgtcaccgcc acatttacct gtgccgggca ggagtttacc
    13381 gccaagggca agaccgtcct ttcccccggc tggaaagaga ttgaacgccg gttccgttct
    13441 tccttaaaga ccgacgccga cgaggacgcc gaggcggtgc gggagctgcc ggagctttgc
    13501 gagggccaga cctttgcaga cgtggccgcc tctgtcaccg agcatttcac cacgccgccc
    13561 aagccctaca ccgaagatac gcttttgtcc gctatggaac gggccggggc cgaggatatg
    13621 ccggaggatg ccgagcgcaa gggattaggc acaccggcca cccgcgccgc cattctggaa
    13681 aagctggtgc agatgggctt tgtccagaga aaaggcaagc agctcattcc caccaaggac
    13741 ggcatcaatc tggctgtggt gctgccggag gcgcttactt ctccccagct tactgccgaa
    13801 tgggaaaacc ggctgaccga gatcgccaag gggcaggccg atcctgacga atttatggac
    13861 ggcatcgaga cgcaggcgcg ggagctggtg cagacctatt cctgtatcag tgaggaaaag
    13921 cagaaattgt ttcaggccga gcgtgttcct atcgggacgt gtccccgctg tggtgaggct
    13981 gtctatgagg gcaagaaaaa ctactactgc ggaaaccggg cctgccagtt cgtcatgtgg
    14041 aagaatgacc gattttttga ggaacgcaag aaagcgttca ccccgaagat cgccgctgcg
    14101 ctgctcaaaa ccggcaaagt caaggtaaag ggcctctatt ccgtaaagac cggcaaaacc
    14161 tacgacggga cggtgctttt ggctgatacc gggggcaagt acgtcaacta ccgcattgaa
    14221 cacaggagct aaaggacagt aacacagcaa gcatagggag tgtggctaca ctcccggtaa
    14281 aatcccccgg ccgcgtccct gccgggccgg ggatttttgc ttgttgggga agtccccaac
    14341 cccctatttt ttgaaacaga acagagaaag gagtgaccgc gtgaacgaag tattttctat
    14401 ccaagtccat aaccggcagt ttttggaaca gggcaaacaa ggcgtctggc tggatttgcc
    14461 caccacaacc gaaacgctgc aagccgccct gcgggatgtt ggcattactg ccgacaatcc
    14521 gcaggatttt tttatcaacg ggtattcctg cccggaggac agacaccttg ccattcccta
    14581 tgacatggtg ctggccgccg acgtggacga gctgaatttc cttgccgccc ggctgggcca
    14641 gcttgacgcc gccgagatcg cagagctgaa cgccgccctg caaaatcccc gtggcggctt
    14701 tgagaatatc ggccagatca tcgactacac cgaaaatgtg gattactatg tgcatcttcc
    14761 cgacgttagg ggggatggac agttgggcga ctactacctg aaccgttccg gcatggtgga
    14821 tatgccggag gaatggaaag ccgggattga tacggcgcag tttggacgcc acattgccca
    14881 gcaggaacaa ggcgttttta cccagtacgg ctatctggtg agaagcggcg acgaatggca
    14941 gcgcgtccat gagggccagc cggtgccgga ggaataccgg gtgatgcact acccccagcc
    15001 ggaagctgac cgggaggaac cgcaggccca gcagccggag gcatccgaaa gccccgacaa
    15061 agcgccgccc ccggttatcc cgatcatgct ggacggcatg aacagcaaag aacgcatgaa
    15121 agtgattact gaccgtctgg aaaccggcgt acaggagctt tttgaaagta gccgctatat
    15181 ggcttactta tccgctatgt ccaagatgca cgattacagt ctgaacaata cgctgctgat
    15241 cgccatgcag ggcggttcga ttgtggcggg ctataacagg tggcaggatg aatttgaccg
    15301 ccatgtgaag cggggcgaga aaggcattaa gatactggcc ccggccccgt tcaaggttaa
    15361 gcagcagatg gagaaaaccg acccggccac ccagcagccc gtgatcggcg cggacggcaa
    15421 gcccgtgacc gaagaacagg aagtgacggt tccggccttt aaggtggtga cagtctttga
    15481 catcagccag accgagggca aggattttcc catgccctat atcagtgaac ttaccggcga
    15541 cgtgcagcag tatcaggatt tctttgccgc gctggaacgg acatcaccct atcccataag
    15601 cattgagccg atttcagacg gggccaaggg ccgctgcttc tacgcaaaac agcgcattgc
    15661 catcaacgag ggcatgagcg aattgcagac cctaaagacc gccatccatg agattgccca
    15721 tgccaagctc cacgccattg accgggaggc ccccgccgag gaacaggcgg cccggccaga
    15781 ccgcagcacc cgcgaggtac aggccgaaag cgtggcctat accgtctgcc agtattacgg
    15841 gctggacacg tcggactatt cttttggcta tgtggccgat tggagcagcg gcagggagtt
    15901 atccgagctg aaagcctccc ttgaaaccat ccgggccacg gccagcgaat tgataaccga
    15961 gatcgacggc cattttgccc agcttcagca ggagcgcgag gcccggcagg aacaggaggc
    16021 cgtcacggtt gacgttaccc cggaggcagc ggccccggcg caggccgagg aagaacaggc
    16081 cccggccctt gatccggcag ccgagccgct tgtgactgtg ctatggagcg aaagccccca
    16141 gctaaaggac ggccagcaaa tgccgttgca tgaggccgac gcgctgtttg cccgtctgga
    16201 tgcagcatat ccggcagagg caggctatga caaaaccgcg ttccgcattg actgtatctt
    16261 tcagggcgag ccggacagct acgaggggcg gcaggatttt ggagataggg acggttccct
    16321 gatcgaacat atccagagct accaccaatt ttacgcgcag gacgaaaact ggaaaaagca
    16381 tgtaatcgct acaaccggca tagagggctg ggagcaggac aaggcccatc gggatatgct
    16441 gttaggcgag tttgtcccct atatgaagct gcattgcagc cttgcccaaa tggaacagac
    16501 ggcgcagggc cgcttacagt ccggggaaac cctgacgccg gaggaaacgg cctatttggg
    16561 ggcgcttctc tcttatgtgc aggagtgccg ccccatgctc aatcagggcc agtatcagtt
    16621 gccggagccg ccgaaactgg ctgacttcga ccagagcttg caggactaca aagcacaggt
    16681 acaggccgag atcgcgcagg aggccgccgc agccggtatg accgtggagg aatacgcggc
    16741 ggctggctat gaagcacccc aacaggacac cttttccatc taccagcttc gaggcgggga
    16801 ggaaacccgc gactatcggt ttgagccata cgagcgttta caggccgcag ggctggccgt
    16861 ggacagtgcc aactatgacc ttgtttatac ggcaccgctg gataaaagca cgtctttgga
    16921 ggatatttac cgcacattca acatcgacca cccggcagat tttaggggac acagcctttc
    16981 cgtttccgac gtggtggtgc tgcaccagaa tggacaggac accgcccact atgtagacag
    17041 cttcggctat atggaagtgc cggagttttt gcgggaaaat cccctgcgta ccgccgaact
    17101 gtctacggag cagaacgaaa acatgattga cggcattatc aacaatacgc cgcccgaacc
    17161 gcagccggag gaacccgaac agctatcttt tgaggatgtg gcagttgctt ccggccagac
    17221 acaggaggaa cccccggagg ctgcgccccc gcaaacggct gtccgctatt atcccatcaa
    17281 tgagggagcc gcccgccgcg ccaaggaagc catgagcttt tctgactacc ggcccggcag
    17341 cgccacggcg gaataccggc actatgtcga tcaggccgtg gaaattgcgg agcgccagaa
    17401 aaagcgtgtg gagccagagt atcacgaaaa gatagacagc ctccttgaca cctacgcccg
    17461 caagctggcc gagaacatga ataagggcta tgagattacc gcccgcgtcc cctctgtcct
    17521 gattgccggg ccgtccaatt tcccggtacg ggcgaaagaa aagcagaacg ccgccagtga
    17581 ccgcaacatg gaggaatttc agtacattca gggcttgctt gacaaaatcc gcagcaccgg
    17641 catgggtggt atcagcgccg acgatccgca ggccgtttcc aagctggaaa agaaactgga
    17701 aaagctggaa gcgtcgcagg agttgatgaa ggccgtcaat gcctactacc gcaagcatgg
    17761 gacgctggac ggatgccccc atctgatcga aagaggcatt gaaaacctga aagcggatat
    17821 ggcgtcgggc tggcactatg agaaaaaacc gttccagtcc tggcagctct ccaacaataa
    17881 cgccgagata cgccggttaa agggccgcat tgaggaattg acccgccaaa aggaggcggc
    17941 ctatgtggga tgggagtttg acggcggcac tgtggagatc aaccgggtgg ccaaccgcct
    18001 gcaaatcttc tttgagggga agccggatgc tgctgtacgc gacgagctga aaagcaacgg
    18061 tttccgctgg tcgcccaagg ccggggcatg gcagcggcag ttaaacgaca ccacaatccg
    18121 cgtcgctgac cgtatcaagt gcatccagcc cctttccggc gaaaagccca gcgccttaca
    18181 gcttgcggcc cgcagggaga aagaaaagcc ctctatccgg gcgcagctca acgcggccaa
    18241 gaccgaacag gccaagcagc caccggcgcg ggaaaaatca aagggattgg aggtagagta
    18301 aatgcagaag tttgaaaatg tggacgttct ggccgcgctg gaacagatca tgcggcagaa
    18361 caccgcgttt tatcaaagtg actttggcat tgacaaagac attatcaggc gggatgccgc
    18421 cagcgatcag gccgcagaca agaccctgct ctggatgtcc cggccctccg ggacgtactg
    18481 tttccgggaa cgtgatgttt tcctgaaaga cacccgacag tacaacacat ggaaatttta
    18541 cggggagcag acccgtgaca agatactggc ctacgcggtg gagctgaccg gcacacaggg
    18601 cggcactatc cggggcaatc tctatgagct ggactatccg cagcatttcc ggcacgtcat
    18661 tgaaaccgcc cagccggtga gtgtgaaccg gctcttttat gagcatggca cccgtgacat
    18721 cccggagagc cagcgttttg acggaagccc tgaccgggta ttgggaaact ttttgtacta
    18781 tgaggcccag ccccatgacc cggctgtgct gcaggaggcg ctacggacgg agcagcacag
    18841 ccgggggcgg gccgtccccg gcgatttcaa agtccacgtc accgctttgc atgacagccg
    18901 gattgaaacc gaggcccggc gtattgtgga cagcctgaaa gatctgtccg cgcctaacag
    18961 ccccaataaa acgcatttca tggtggagat ctccccgcac tttgcggcga ttgcttccac
    19021 caaggataat gaccggctct ttgccatgct gccctataaa tccctgtgtt ttaccgggat
    19081 gaaaggcagg catggcgtgt atgccgtgat taaccgggac gagcgccggg atgtgagtat
    19141 tcgcaggccc cggccctcta tccgcaagca gctatccgac agcaaacagg ccgccagccc
    19201 aaaaaaggcg gcggccagaa ccaaaaagaa tgaattggag gtataaggaa tgatgcactt
    19261 taccgtggag gaagaaaacc tgatctgtat gtaccacaat gcagaccggc gcagaacgat
    19321 aaacaggctc accgccgtca tgccggttat ggatggggat atgcgggcgc aggcccagca
    19381 gaccctttcc aagctggaac gcatgacgga tgccgactat gacggccaga agttccactt
    19441 cacagacgaa taacgaacag cagacgccgg gcgcggcggc catgtggtcg cttgcgtccg
    19501 gcgtcttttc tttttgccta ttcctacgca tttagaaccc ggtattgtcg gggataggat
    19561 gaagtattcg cttgcccctg caaacatgcg ggagaagcct tgcagcgggc ggttttcaat
    19621 cccttaaatg cgtaggttgc agctataatt agacccctga aacagatata gccgcgcagc
    19681 gggtgtatct gtttcagggg tgtgcaggtg atagccgcaa agcggcgtaa gagggtgcag
    19741 ccctcttgcc gtctatctct gttccagcgg gaagctcggc aatgcttcca gcagtttcag
    19801 ggaaatgcgc tgccgtatat cttcgtcaat gactttcttc tttttgccgt ttgcctgtct
    19861 gatctccacc gtggaaagct cgttgatgta gtcagcataa tgggccaaca ctttttccgt
    19921 ggcccacctc tcaccggcaa cggcggcttt gatcgtttca tagtccagca gcttttcttg
    19981 tccgtccatc ctgatacccc cgtcagactg attttttcta tgataccata ttcggaagca
    20041 tggaaccaga gaaaacggcg cttttctgct ggtaaattct acgcatttag aacagcactt
    20101 cggcggggat aggataaaac acccacatac cccgctgacc atgccggaaa agccttgaaa
    20161 tataagggct tttacagctc taagtgcgta ggtttccagt atcttttccg ctggcgctcg
    20221 gcctctttgc ggcggcgcat ccgtttggag caggccgcgc agtatttggc ccggttagac
    20281 ttcggcgtga acgtggcccc gcactcacag cagcgtttca cttcgtcccg gtctttggtg
    20341 atctcggcat acagggccgc atccagcggg aggacagcgg ccttaaacca gcggcacaga
    20401 agcgaatagg aaatgctctg tacgcaaata cattcttcgc cgtcgtccag caacaggcag
    20461 ttgccgtcac aatagttgca gcactcatgc gtcagcttcc gggccttgcg atattgaggg
    20521 taattcattc gtggaatact catagctctt gcgtccattc ctttcccggc gtcatacgca
    20581 aaatgccgtc cacattctgc ttgatgatgc cgtattcctg catctgcttt ttcgcttcgc
    20641 cgtacttctc atacagccgt tccttttcct cggccaatcg cttgtactcg gctttcaggg
    20701 cgtacagatc ggggagcttt ttcacaccct gtccctttag ggcctttgcc gcagcttcat
    20761 acaaaatcaa cgtgctttca tgctcacgcc ggaattttgc cttatccgct gcattgcggt
    20821 attccatagc cacgggccag agctgcttgt aggtagaaat attctttatc agcaccgcca
    20881 tatccgcaag ccggtgttcc atagctttga gggtagcggc ggcctcgtcg ctggcggcgg
    20941 tgatctcggc cactttgctt tccaaatccg ggtaggtgtc tatcccatgt tcagtcagga
    21001 aattcaaagt ctttgcggcc tgcttcaaat tgtggagctt cgcccacctc tcatagcctg
    21061 ccgactgctg ggccttaatg ttgttttcca gatcaatccg caaagaaatc cccttgcgct
    21121 ctttgggcgc tctggtgata acgcggcctt tgatacgctc tttgatggct tcctcggtgt
    21181 aggcttcccc caatgtcttg cagcgggtaa agcgttcctg tccgggagcg cggaaagaaa
    21241 taaactttcc cggcttgatc tcatagccct gcgcctgcat gagccgcagg aaatcatcaa
    21301 aatcttttgc ctgcgggata gcggcgtcaa tggctgcttt gagcttcgct ttccagctcg
    21361 tccctttccg ctgggcgtcc cactcggcat agctcttgcc cttactctgg ccgggcttca
    21421 ccacggacaa tccgttttcc ttgcacagcc tgtcgctggt gcgccggata taggcatagc
    21481 tctgtttgtt ggaaatgtac ttgcgctgat ttaccatatc cacggcacag aaaataatgt
    21541 gattgtggac gtggccctta tcaatatggg tagtcagcac atagggatat ttcccttgca
    21601 gcacttcctc ggcaagctgc ttcccgatct catgggcctg ttccggcgtg gcttcgccgg
    21661 gggcaaagga ttgtatcagg tggtggccca gattattgcc cttttggtat gcctgcgcca
    21721 gcagcatttc aaattcaatg tccgctgttt cataagagca ggcaaaggag gccaccagca
    21781 gcttgccgtc agttttgtcc gggttctcga tgtagtcaag ggcttttttc agcgttgact
    21841 tgataggatg tatctttgta actgccatat ctgcgccagc gcccccttta tatcctcgat
    21901 gtcctgtgcg tacaccggcc ccataccgtt cagcctcttt gcaatctgat tgacgttgac
    21961 gccgatcttc tgtatctcgg cggtctgcgc ccggatgtcg ctggtgtcca tgttgacgat
    22021 atagccgtca atgagcatct tccgggcgta ggctgaaaag ttcttcgtat gcagcagcgc
    22081 cattttctgt tcaatcatag cccgttcctc cggcgtcaca gggacgcgca gcacgatttt
    22141 tcgctttctg tttgccatgc ttcaccgtcc tttccattca ggggtttggg gatttcccca
    22201 acaagcaaaa cggacgcggc ccggcaggga cggggcaggc cgtttttccg ggagtggcta
    22261 cactccctat gcttgctacg ttactgtttc tccactaaca agacgtttcc aaagccccaa
    22321 actacccgca aaatagccct ctggaaagaa aaaagtgcaa aaaaatagaa gctacgctgt
    22381 gcagctcccg ctaaaagtaa aacagaggat gccagttaca catcctccgt ttcattcttc
    22441 ttcggctttt caattagtat ggcgctgtca atcgcgccct cgatgatcgg caggtattct
    22501 tcggggcaga gtttcagctt gtggctgacc ctttgccgca gttcgctttc ctcccgcatg
    22561 atctccgggt taaagtagcg ttccacagga agcccgcagg ctttaataag ctgtatgaca
    22621 accggcagac tgggaatggc ccgtcgtttt cgatgttggc aagatagcgc cagtcaatat
    22681 ttattttctc tgccattgct ttgcgcgaaa cgcgctttgc ttgccgggcc gctttcacat
    22741 ccgcgccgaa agtttcaaag ccgggacaat cttcaatatt cgccatatcg cacctcccac
    22801 ttacattgta tatttcatac tctgcccgtg gaatgttggt atatgcacct ttcttaaagt
    22861 ttatatttca cattttgttt cttttattct tcgagcaaaa gaattataat gtaccctgtg
    22921 gaggtgtact atggactaca tgacgttgaa agaggcaggc gagaaatggg gtgtgccccc
    22981 acgatggata aactactatt gtgctgcggg acgcatcccc ggcgctgtaa agatggcgac
    23041 gatctggctc attcccaaaa ctgcggaaaa gccgattgac aggcgtacaa agaaaggaaa
    23101 ggcggttact gaatagtata atgaaaccgg cgcattttgg atatggtttt gaagagagag
    23161 aaagcatggt attttgtcac ggaaaaacta taagatgata gatggcaggc tgctccagac
    23221 cgataaaaag tattctcatc tgaaacagaa agaaaagatt tacacatgga tgtatgaaga
    23281 agcgaaactt tatcatgaca gaaatggaaa atgcccgaaa caaagacgag gaagctgctg
    23341 ctgtatcagc cgtatatggt agaattgaaa aggccggcat ctggattctt tatggtgaag
    23401 tctccagaca ttggataact gctggtctaa tgcgcctttg agtatggggt gattgatttt
    23461 aataccgggg aggtacggct ttttgaggaa aatattctgg gatttacatt tggcgattat
    23521 tacatttcct gtaactgcaa ggacacggat gatgaagata tgaggaatat agacttgctg
    23581 attttctact gtcttgaacg gtattaggac tgaatctgct gcgcggagtg cggcaagagc
    23641 gaccggaggg agtacacaca ggccctgcct gtgtgaaaag ccataccgca aggtgcattc
    23701 gcaaaaaaga ggccgcgcat tttgctgagg cttggggtgt ccccaacaag caaaacgaga
    23761 acgccgcagg cgggcaagtg gtggatcaga atatccgtaa aggatattct gatccattgc
    23821 ttgccagtat tatcgggatg ttttgacaac cgctgtaatc gaaacatccc aaacattaag
    23881 aaggtgttaa ttattattat cttgatttaa ttcgtttaaa ccaataatat agatacatac
    23941 accatgaaaa gaagcgagta tcccaatgac ggatgattaa aatccgaaaa gaaatcgctt
    24001 tgaaactaca gggaaactac agactgttat gttatcttct taaaatggag ggatttttat
    24061 gtcgatacga attctacttg tcgaggatga tgatcatatc tgtaatacag taagggcgtt
    24121 tctggctgag gcaggatatc aggtggatgc ctgcacagat ggaaatgagg catacaccaa
    24181 gttttacgaa aacacttatc aactggttat tcttgatatt atgctgcccg gtatgaacgg
    24241 gcatgaactt ttgcgtgaat ttcgtgcgaa aaatgatact cccattctga tgatgacagc
    24301 cctgtcggat gacgaaaacc aaatccgggc gtttgatgca gaggcagacg actatgtaac
    24361 aaagccattc aagatgcaga ttttactaaa gcgggtggaa gccctgctgc ggcgcagcgg
    24421 tgcgctggca aaggaaatcc gtgtcggcag gctgacactt ctgccggagg atttcacggt
    24481 actttgtgac ggtacagagc tgcccctgac acgaaaagaa tttgaaatcc ttttgctgct
    24541 ggtgcagaac aaaggcagaa ccttaacgca tgaaatcatt ttgtcacgta tatggggata
    24601 tgactttgag ggtgatggca gcacagtcca cactcatatc aaaaatttgc gggccaagct
    24661 gccggaaaat atcatcaaaa ccattcgcgg tgtaggatac cgattggagg aatcataata
    24721 atggaaagaa aagggatttt cattaaggtt ttttcctata cgatcattgt cctgctactg
    24781 cttgtcggtg taacagcaac actgtttgca cagcaatttg tgtcttattt cagagtgatg
    24841 gaattacagc aaacagtaaa atcctatcag ccattggtgg aactgattca gaatagcgat
    24901 aggcttgata ttcaagaggt ggcagggctg tttcactaca ataaccaatc ctttgagttt
    24961 tatattgaag ataaagaggg aagcgtactc tatgccacac caaatgccaa tacatcaaat
    25021 agttttagac ccaactttct ttatgtggta catagagatg ataatatttc gattgttgct
    25081 caaagcaagg caggtgtggg attgctttat caagggctga caattcgggg aattgttatg
    25141 attgcgataa tggttgtatt cagcctttta tgcgcgtata tctttgcgcg gcaaatgaca
    25201 acgccgatca aagccttagc ggacagtgcg aataaaatgg caaacctgaa agatgtaccg
    25261 ccgccgctgg agcgaaagga tgagcttggc gcactggctc atgatatgca ttccatgtat
    25321 gtcaggctga aagaaaccat cgcaaggctg gaggatgaaa tcgcaaggga acatgagttg
    25381 gaggaaacac agcgatattt ctttgcggca gcttctcatg agctaaaaac gcccatcgcg
    25441 gctacaagcg ttctgttgga gggaatgctt gaaaatatcg gtgactacaa agatcattct
    25501 aagtatctgc gcgaatgcat caaaatgatg gataggcagg gcaaaatcat ttccgaaata
    25561 ctggagcttg tcagcctgaa tgatgggaga atcgtaccca tagctgaacc gttggacata
    25621 gggcgcacgg ttgccgagtt gctgcccgat tttcaaacct tggcagaggc aaacaaccag
    25681 cggttcgtca cagatattcc agccgggcaa attgtcctgt ccgatccgag gctgctccaa
    25741 aaggcactat ccaatgtcat attgaatgca gttcagaaca cgccgcaggg aggcgaggta
    25801 cggatatgga gtgagcctgg tgctgaaaaa tgccgccttt ttgttttgaa catgggcgtt
    25861 cacattgatg atactgcgct tccaaggctg ttcaccccat tctatcgcat tgatcaggcg
    25921 cgaagcagaa aaagtgggcg aagcagaaaa agtgggcgaa gcggtttagg acttgccatc
    25981 gtacaaaaaa cgctggatgc catgagcctc cagtatgcgc tggaaaacac ctcggatggc
    26041 gttttgttct ggctggattt accgctcaca tcaacattgt aaatatttaa aacttaaatg
    26101 attttgggct ctttgtcaaa taggactgat agagtgagat aagaacatct aaggtagaat
    26161 tccttttcta ctttagatgt tttttgattc ataggtaatc agattattta gtaagaaaat
    26221 tctggtcttc attttccgaa agcttcgaaa gccataagcg actcgtttca atgctttgat
    26281 gtgggtatgc agattttcta ttttggcatt tgaataaggc aattcgaggg ctaaacgaat
    26341 gccggattca tagtttataa aagctttttt tatagaatgc agttcttttg gaacggaatt
    26401 gggcatcgtt cttacaagtt taaagaaagt gtccgcctct ttgtcgtgaa acgcttgaag
    26461 aatttcttga tagtaaaaat aggcgattcg taattcgtca gagatcgaaa gcagacgatc
    26521 caccacttca ctttcggtga gatacgccca accaaaattg cgccatttgg tccatttgtc
    26581 ataaacaagc ttgtcctgtc gtttgatcag aagccgagac aacgccttta attgtcgata
    26641 atatttggct tgtgttggat cgtttcgatc aaagcttttc atgattcgta cgcgaatatc
    26701 ctcaaattta cgaatgagat gtttgacaat gtgaaaacga tccacaacga gttttgcctt
    26761 tggaaaacat tcctttatca gagaggcata agaaacattc atgtccgtca cgatgacctt
    26821 taccttcaat cgtgcgcgtc gttcaaattg cataaaataa gaaacgagag cctttttctt
    26881 ccgtgaagga agaatatcca gtaattttcc agtatcgcca tccatacaag aaaaggagta
    26941 gggaccgact tgatttttca gtgaacgaaa ttcatccatt aataaacaac gcggtaacca
    27001 atttcttttt acttttgtca tggggatcag agaagcgagt gtccgagaaa cggtcgacca
    27061 agaaacgccg caatgcttag caattgtcgt cattgcttgt ttttcagtta gctcttctaa
    27121 aattttatag cgcacttgtt tagcgataaa gcagtttcta tcgacaatgt aggtattcgc
    27181 cgtaaaatag gtgtggcagc cacgacagtg gtagcgttgt ttcgctaaag caagataagt
    27241 cagttggttc gcacatttat taagttgaat gagcgatttt ttctttccgt tcttaacgat
    27301 catgccttca tgattcatct gtttacacaa aggacagcga cgaatttctt tacttaacgt
    27361 cgcagaaaca acaagactct tgatattctt aatgctcttc gattccacat ttgtgatgaa
    27421 aatgttttta tctttgattc caagtagtag tttgatatga tgaagatcag gcatacagaa
    27481 cgctcctttg attgtgttgt ggtgattcaa ttctaacgga gcttctgtat gttttgtttt
    27541 ttcatgctca aaagggaccg atagaatgat tttctatcag tcctagaaat tatagagccg
    27601 attttgaccg acaggtataa ccctgccggt ctttttttgc tgctacagga aaactacaga
    27661 ctggctacag ggaaagtaca gatgcgcttg ccataataac aatcgtacca gccacaaatc
    27721 gtagttttat tgcaaaggag gcattcgatc aaatggaaaa aagcaactat cattccaatg
    27781 cggatcatca caaacggcat atgaaacaat ccgtggaaaa gcgggctttt ctatgtgcgt
    27841 tcattatttc gttcacagtc tgcacgctgt ttttggggtg gagactggct tccgtattgg
    27901 aggcaacaca gataccgccc atccctgcaa ctcatacagg cagcagcact gacgtagtgg
    27961 agaatttgga ggaaaacgct cttgccaccg ccaaagaaca gggagatgaa caggaatgga
    28021 gcctgatttt agtgaacagg cagaacccca tccccgcaca gtacgatgtg gagcttgagc
    28081 aactatcaaa tggtgagcgg atagatattc ggatttctcc ctatcttcaa gatttgtttg
    28141 atgccgcaag aactgatgga gtttacccga ttgtcgcatc cggataccga acaacagaaa
    28201 aacagcaaga aattatggat gaaaaaattg ccgaatataa ggcgaaaggc tacacctctg
    28261 cacaggctaa agcggaagca gaaacttggg tggccgtgcc gggaacgagc gagcatcagc
    28321 ttggtcttgc tgtggatatc aatgcggacg gaattcattc aacaggcaac gaggtttata
    28381 gatggctgga tgaaaacagc tatcgttttg gttttattcg tcgctaccca ccagacaaga
    28441 cagagataac cggtgtgagc aacgagccgt ggcattaccg atatgttggc atcgaagctg
    28501 ccacagagat gtacaaccaa ggggtttgcc ttgaggaata tttaaaacca gaaaaatgag
    28561 aaaaggatgt tatgttatgg acagaaaaag attgacacag cgtttcccgt tcctgcttcc
    28621 aatgagacgg gcgcagagaa aaatgtgctt ttatgcggga atgagatttg acggctgtcg
    28681 ctatgcgcag acgatagggg aaaaatcgct ttcccatttg ctctttgaaa cggattgcgc
    28741 attatacaac cacaataccg gatttgacat gatataccag gaaaacaagg tgttcaactt
    28801 aaagctggca gcaaagacct taaacggctt attgataaga ccgggggaaa ccttttcttt
    28861 ctggttgttc ctgcatacgc cattgacaat tatccagcgc agaggtcacg aagtaaagga
    28921 gtttccagag ccaaacagtg acgagattaa aggggtggat gcaaccattt cagagggctg
    28981 gattgattta aaagtgcgaa acgataccga ctgcacctac caaatatggg tgaccttaga
    29041 tgatgagaaa atcatcggtc aggtgttcgc cgacaaagag ccccaagcat tatacaaaat
    29101 tacaaatggc agtatccagt atgtccgtga aagtggcggg atttatgaat atgcccaggt
    29161 taaacggatg caagttgcct taggtaccgg ggaaataata gattgcaagc tgctttatac
    29221 aaacaaatgc aaaatctgtt atcccctccc ggaaagtgtg gatattcagg aggagaacca
    29281 atgagaaaaa gtgtgggcat tactgttttt ggatgcgaac aggatgaggc aaatgctttc
    29341 cgcgctttat cgccggattt tcatattatc cctacgctga ttagcgatgc gatatcggca
    29401 gacaacgcaa aattggccgc tggcaatcaa tgcgttagcg taagccataa gtccgaggtt
    29461 tccgaggcga caattcttgc gctgagaaag gtcggggtaa aatacatttc tacccgcagc
    29521 atcggctgcg atcacattga tacgactgcc gccgagagaa tgggaatctc ggttggcacg
    29581 gttgcgtatt cgccggacag cgttgcggat tatgctttga tgctgatgct gatggccata
    29641 cggggtgcaa aacccaccat gcacgccgtg gcgcaacaag atttcagatt ggatcgtatc
    29701 cgggggaaag aactggggga tatgactgtg ggagttattg gaaccggcca tatcgggcaa
    29761 gcggtcgtca aaaggctgcg gggatttgga tgccatgtgc tggcctatga taacagccga
    29821 aaaatggatg cagattatgt ccagcttgat gagcttctaa aaaacagcga tattgttacg
    29881 ctccatgtgc cgctttgtgc ggatacccgc catctgatcg gtcagaagca aattggagag
    29941 atgaagcaag gcgcattttt aatcaacacc gggcgcggag cgcttgtcga taccggggcg
    30001 ctggtggagg cgttggaaag cggaaagctg ggtggtgcgg cactggatgt gttggagggc
    30061 gaggatcagt ttgtttatac cgattgctcg cagaaagtgc ttgaccatcc ctttttgtcg
    30121 cagcttttaa ggatgccgaa tgtgatcatc acaccccata cggcgtacta cactgagcgt
    30181 gtgctgcagg atactacaga aaaaacaatc aggaattgtc tcaattttga aaggagttta
    30241 cagcatgaat agaataaaag tcgcaatcat cttcggcggt tgctcggagg aacatgatgt
    30301 gtcggtaaaa gccgcaatag aaattgctgc gaacattgat acggaaaaat tcgatccgca
    30361 ctacatcgga attacaaaaa acggtgtatg gaagctatgc aagaagccat gtacggaatg
    30421 ggaagccgac agtctccccg ccatactctc cccggatagg aaaacgcatg ggctgcttgt
    30481 catgaaagaa agcgaatacg aaacacggcg tattgatgtg gctttcccgg ttttgcatgg
    30541 caaatgcggg gaggatggtg cgatacaggg gctgtttgta ttgtctggta tcccctatgt
    30601 gggctgtgat attcaaagct ccgcagcttg catggacaaa tcactggcct acattcttac
    30661 aaaaaatgcg ggcatcgccg ttcccgaatt tcaaatgatt gataaaggtg acaagccgga
    30721 ggcgggtgcg cttacctacc ctgtctttgt gaagccggca cggtcaggtt cgtcctttgg
    30781 cgtaaccaaa gtaaacggta cggaagaact taacgctgcg atagaagcgg caggacaata
    30841 tgatggaaaa atcttaattg agcaagcgat ttcgggctgt gaggtcgggt gtgcggtcat
    30901 ggggaacgag gatgatttga ttgtcggcga agtggatcaa atccggctga gccacggtat
    30961 cttccgcatc catcaggaaa acgagccgga aaaaggctca gaaaatgcga tgattacagt
    31021 tcccgcagac attccggtcg aggaacgaaa tcgggtgcag gaaacggcaa agaaagtata
    31081 tcgggtgctt ggatgcagag ggcttgcccg tgttgatctt tttttgcagg aggatggcgg
    31141 catcgttcta aatgaggtca ataccctgcc cggttttaca tcgtacagcc gctacccacg
    31201 tatgatggcc gccgcaggaa tcacgcttcc tgcactgatt gacagcctga ttacattggc
    31261 gttaaagagg tgataagtat ggaaaatggt tttttgtttt tagatgaaat gttgcatggc
    31321 gtccgttggg atgccaagta cgccacatgg gataacttca caggaaaacc ggtggatggg
    31381 tatgaggtga atcgcatcat cggcacaaag gccgtggcgt ttgctctgcg cgaggcacaa
    31441 atccatgcgg ctgcgcttgg ctatggcttg cttttatggg atggatatcg gccaagaact
    31501 gcggtggact gcttcctgcg ttgggcagcg caaccggagg acaatctcac aaaagaaaaa
    31561 ttttacccca atatagagcg agccgagttg attacaaagg gttatgtggc ctcacaatcc
    31621 agccatagcc gtggaagcgc aattgatctt acgctctacc acctggatac aggggaactt
    31681 gtttcaatgg gaagtaactt cgattttatg gacgaacggt cgcatcatac agcaaaaggg
    31741 ataggggatg cagaggcaca aaatcgaaga tgcttgcgta aaatcatgga aagcagcgga
    31801 tttcagtctt atcgctttga atggtggcac tataagttga ttgatgagcc ataccccgat
    31861 acctatttta attttgccgt ttcataatga aagtattggt tttgtatcag attttggatt
    31921 tgataaaatc tatgctggtc atggacaatc ttttactgga aagtggttaa agcatctttt
    31981 gtaatacatt gcaaattata gtattcaatc tgcccagcgg cagaaaagaa aaaaaccgct
    32041 gttgggcaga cctttcccac gcccattttg tattttgctg gcggcggttt tgagaaatca
    32101 aggccgccgt tcttttctgg cagcaaaagg tgtggcggcc cataggagga tgccgctatg
    32161 ctgttttgct attgccatgc tctaatccac ttccaccact caaaaaaagc cttgcccctc
//
LOCUS       DASJSZ010000027        30148 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.027, whole genome shotgun sequence.
ACCESSION   DASJSZ010000027 DASJSZ010000000
VERSION     DASJSZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.027"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1171)
                     /locus_tag="U4B03_001158"
     CDS             complement(<1..1171)
                     /locus_tag="U4B03_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2068026.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSF"
     gene            complement(1749..1892)
                     /locus_tag="U4B03_001159"
     CDS             complement(1749..1892)
                     /locus_tag="U4B03_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEN2068027.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2115..2594)
                     /locus_tag="U4B03_001160"
     CDS             complement(2115..2594)
                     /locus_tag="U4B03_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2068028.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(2667..2951)
                     /locus_tag="U4B03_001161"
     CDS             complement(2667..2951)
                     /locus_tag="U4B03_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068029.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3029..3223)
                     /gene="ssrS"
                     /locus_tag="U4B03_001162"
     ncRNA           complement(3029..3223)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U4B03_001162"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3295..4581)
                     /locus_tag="U4B03_001163"
     CDS             complement(3295..4581)
                     /locus_tag="U4B03_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEN2068030.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            4719..5390
                     /locus_tag="U4B03_001164"
     CDS             4719..5390
                     /locus_tag="U4B03_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEN2068031.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5356..5844
                     /locus_tag="U4B03_001165"
     CDS             5356..5844
                     /locus_tag="U4B03_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEN2068032.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            5857..6804
                     /gene="prmA"
                     /locus_tag="U4B03_001166"
     CDS             5857..6804
                     /gene="prmA"
                     /locus_tag="U4B03_001166"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEN2068033.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            6805..7569
                     /locus_tag="U4B03_001167"
     CDS             6805..7569
                     /locus_tag="U4B03_001167"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEN2068034.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            7802..10015
                     /locus_tag="U4B03_001168"
     CDS             7802..10015
                     /locus_tag="U4B03_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEN2068035.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10026..10472
                     /locus_tag="U4B03_001169"
     CDS             10026..10472
                     /locus_tag="U4B03_001169"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEN2068036.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            10643..11902
                     /locus_tag="U4B03_001170"
     CDS             10643..11902
                     /locus_tag="U4B03_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEN2068037.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(11991..12938)
                     /locus_tag="U4B03_001171"
     CDS             complement(11991..12938)
                     /locus_tag="U4B03_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2068038.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13332..14624
                     /locus_tag="U4B03_001172"
     CDS             13332..14624
                     /locus_tag="U4B03_001172"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEN2068039.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            14639..16408
                     /gene="aspS"
                     /locus_tag="U4B03_001173"
     CDS             14639..16408
                     /gene="aspS"
                     /locus_tag="U4B03_001173"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEN2068040.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            16517..17392
                     /locus_tag="U4B03_001174"
     CDS             16517..17392
                     /locus_tag="U4B03_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2068041.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            17658..19541
                     /locus_tag="U4B03_001175"
     CDS             17658..19541
                     /locus_tag="U4B03_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN2068042.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(19616..19900)
                     /locus_tag="U4B03_001176"
     CDS             complement(19616..19900)
                     /locus_tag="U4B03_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2068043.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(19890..20624)
                     /locus_tag="U4B03_001177"
     CDS             complement(19890..20624)
                     /locus_tag="U4B03_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEN2068044.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            20753..21376
                     /locus_tag="U4B03_001178"
     CDS             20753..21376
                     /locus_tag="U4B03_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN2068045.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            21568..22689
                     /locus_tag="U4B03_001179"
     CDS             21568..22689
                     /locus_tag="U4B03_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEN2068046.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            22696..25824
                     /locus_tag="U4B03_001180"
     CDS             22696..25824
                     /locus_tag="U4B03_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEN2068047.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            25843..29037
                     /locus_tag="U4B03_001181"
     CDS             25843..29037
                     /locus_tag="U4B03_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEN2068048.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29183..29890)
                     /locus_tag="U4B03_001182"
     CDS             complement(29183..29890)
                     /locus_tag="U4B03_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2068049.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         9873 a         5462 c         6394 g         8419 t
ORIGIN      
        1 aaaagctact acctaatttg atggtgccac cgtaaggctt ttcctcaccg agtaataatt
       61 tgagtaaggt tgattttcct gcaccattgt cgccgatgat ggctaaatga tctccacgat
      121 agactgcaaa actgctgtct ttgaatagta cttgatcagc gtactgctgg tgcaagtttt
      181 gaatgatgaa aacttctttg ccagattttc caccattaga caagctttgc agccgatttt
      241 tcgggggagg tggcggtttg acgagtgtca gtttcgccag gcggcgttcc agttctttgg
      301 cttttttgaa gaaggactca ttgtctcctt catgtgccca ctgacgaaaa cgacgaatca
      361 tgactttcaa ccgctggact tctttttgct gcagttcgta attttttgtc agctcagcca
      421 agcgagcttc tttcaagact cgaaaacggc tgtagtttcc gggatactca atcaatgcac
      481 cttcttccag ttcccatgtt tttgtggtga cttgatcgag aaattgccga tcatgagaaa
      541 cgaccagaac cgattttttt gtattctgta aatagttttc caaccagcga atactgttaa
      601 gatctaaatg attggtcggt tcatcgagta aaagtaaatc ggcatcggct gctaaaattt
      661 tggctaaagc gactttaact cgttcgcctc cgctaagttc tgttaaccgt gcttcctgac
      721 ggtgtggaat ccctaagcct tgcaagatcg ctgcaatccg gtcttctaat acgtaaccgc
      781 ctagttcttc aaagcgatgc tgcagctcac cgtaacgggc cagacacttc tctaagtttg
      841 tatctggttc tgtcatcaat gcttcgtagc gactcatttg ctgtttgagg tcctgtatct
      901 cagcggcaga atggagcaag tagtggttga cggtcgcttc ggaaactgta agttcttgtt
      961 cgaccgtccc gatctttaag ccttttttgc gactgataac accgctgtcg acaccttctc
     1021 tgccggtcaa gatttgtaaa aaggttgatt tgccagttcc gttgataccg actaagacaa
     1081 ttttttcacc agcttcaatt tgacaggata aatcctcaaa taatggattc ccgccgattg
     1141 tttttttaat agaaattgct tgtatgatca tgatctctct ccttgagacc actcaaaaag
     1201 gccatggtta tccatggcct gtgttattta aactcagatt atcatgagaa aaataccttg
     1261 agatggccat tgattttacg tgaattcaac tgttttggac aaacttgtcc cgtcaacagc
     1321 aactgtaaaa ccaattgcat ggctaaatgc gatatggtat cgttggtaat tttcattact
     1381 tgttatttta gtcatgcgca tcatagtatt ttccctcctt cgtgtagttg tcttttccag
     1441 tgtacctgaa agtgtcagaa agttcaacta gcgctgacat ttacttaata tttatcgatg
     1501 tgtaattctg tcagagagct ttttttatct gactgagggt aatgtctcgt ggaccacatc
     1561 attgtattct gagctaaacc agcgttcagg cgaaatcata tggtgtttga taaaaaactt
     1621 tgtctacatg acttcctttt gccatttgct taataccgtt gcattaaaca gctcttctct
     1681 ttggaaatct caccaacatc gcgttcagga gtatcgttgt ggttgcaatg gcaagcggta
     1741 gcctttcatc aattcttgaa tgatttaccg aatgtagcac ttgccttctc gcggtacccg
     1801 aacccgaaat aaaattttag tttggcgata aaatcggtat ccgccatcga tacttcatat
     1861 tttttcgtat cagattcatc ggaaacttcc acattgacga accaataagc tttggcatgc
     1921 ttaggtcgtt ttaaaccaca ccagggcgac taatcgccct ggtgttcact tttttttatt
     1981 ttacttgaac tcattgactg ttttcccttt attttaaaca ttataaagac cgatgagtgc
     2041 ttgccgcgtc ttttgttttt atcacggttt tttgtgcaaa aaaacctgcc gatagttggc
     2101 aggtcgtcta cgtgttaagc tttggtgtgg tctttagctg gcaattggtt ttctaaatca
     2161 gttcggacaa caagcacatc gcaagctgcg tttctaataa catattcaga aacagaacca
     2221 ataaataaac gttctacagc attcaatcct gtggctccta acataatcaa atctacttga
     2281 ttatcttctg ggatttctct ggcaataatt tgttttggtg agccatattc tacgacagag
     2341 gttacattat tcaagccagc tttcttcgca ttgctttcat aatctgccaa tgtttgcttt
     2401 gccatttcag tagcttgttc tgcaagcatt ccatcaaagg atgaaacggt ttggaaagaa
     2461 cgtgtgtcaa tgacatgtgc taatagaagt tctccgttat ttcggatagc tacattgacc
     2521 gcctttttaa aagctaattc agcttcatca gaaccatcta cggcaaccat gatttttttg
     2581 tattgttgta acataagcat tcactcctca agtcaatcgt attgtactct tttattgtag
     2641 aacactttag ggaaaaagag aattatttac tgctttggtt cagcaatttt ttttgcaaat
     2701 cggatactga aggtggcaga aacaataatt acgatcagta aaaagaccaa ggcaataaag
     2761 cgttgattga aaaaagctgt taaaataccc attacgccta aaacagcata tgcagctccg
     2821 taaaattgta ggaatccttg atttttatca tttttgttga tcaatacaaa aaagatatcc
     2881 tgtttattaa ataaataata gctaacgaat aataatacta aagtggcgat aatcattaaa
     2941 atcctaatca tgaacaaatc ctccaaaata agcaacaaat agaaaaagca atttgccaag
     3001 agcaaattgc tttattcttt aaaagatggt aatctccgtt aatgccgttg tttgtcctgt
     3061 agtattgatc ccgcgaattc aagcaggtgg gtcccatggt cgtagcttcg aactaccaaa
     3121 tgataaggca tgttaccagc agtgacacaa gacgggtccc aagatatcaa taaaaatgtt
     3181 cggtcaacac acaaagagga caattcgaac atcacagatt tcccacctct atagtagcat
     3241 atgaatttaa aaagagcaat gagagagctc gcagaaacag aaccgttttc ttgtctagtt
     3301 ttttggagga tgctttttcc actcttttat acggtaatac tgttgaccca gagcctgttc
     3361 atatttccct gtatcttttg gctgataata ctgagcattc ttcagtttat ctggtaaata
     3421 ttgctgattg acccaagcct gatcaaaatg atgaggatac tgataaccca cgccacggtt
     3481 gagtgatttc gcacctttgt aatggctatc tctcaaatga tcaggaacat ctccagcttt
     3541 gccctctcga atatcagcaa gtgctgcatc taatgccatg taggcagaat tggattttgg
     3601 cgacaagcaa agatcgacga cgacgtctgc tagtgggata cgtgcctcag gcaatcccaa
     3661 tttttcagca gctaatacag cgttcactgt acgagccgca gcagcaggat tacctaaacc
     3721 tatatcttca taaccgataa ccatcagacg ccggcaaata cttgccagat cacctgcctc
     3781 aactaaacgg gcgagataat gaagtgcagc atccacatca cttccacgaa tcgatttttg
     3841 aaaagccgaa ataacgtcat aatgagcatc tccatttttg tcatgggtaa gagcttttct
     3901 ttggacacat tcttcaatga tcgacagtgt aagatgaatc cttccttctt tatctgaagg
     3961 agtcgatagt gtagccagtt ccagcccatt caatgcactc cgcaaatcgc cgttcgttgc
     4021 acgcgaaagg tgaagaagag catcttcgtc taactggatt gcttgttgtc caaggccgcg
     4081 ttctttatcc cttaaagcat gttcgactgc caactggatg tcttgttctg ttaatggttt
     4141 gacttcaaag atctgtgtac ggctgcggat cgctggattg atggtaatat aaggattctc
     4201 agtagtcgct ccgatcaaga tgattcggcc actttctaaa tgaggaagca gaaaatcctg
     4261 ttttgtttta tctaatcggt gtacttcatc caataagagg atcactgtac cactcatttt
     4321 tgcttcttcc gctacaactt gtagatcctt ttttgaatct gtagcggcgt ttagcatgcg
     4381 aaatgcataa ttagtagagc cggcgatcgc acttgcgata ctcgtttttc cagtaccagg
     4441 aggtccatat agaatcatgg aagagagcat gcgagcatcg accatgcggc gtatgatttt
     4501 tccttccccg actagatgct gctgaccaac gacttcatcg atcgttcgag gtctcatgcg
     4561 ataagccaat ggctgttgca taggattttc ctccttcttg tttttacgtc ccttaattat
     4621 atcatattta gcaagatgcc tagaaagaac agccttctgt tgttttgttt tcatagctta
     4681 tccaactaat attgtgtatg ataaagtagg tgagaaaaat gacggataaa caaaagatag
     4741 atgaattttt ttggaataaa acgacgccag aagtggcacg ggatttattg gggatgtatt
     4801 tggagcatga cacaccagaa ggaagattag ctggatatat tgtagacgcg gaagcatacc
     4861 taggtccaga agacgaggcc gcccatagct atggtttaag aagaacaccg cgtgtgcggg
     4921 caatgtatga aaaaccaggt acgatctacc tttacacgat gcatactcat cggattttga
     4981 acatcattac tcagccagaa gggattcctc aaggagtaat gatacgtgca atcgaacccg
     5041 ccgcaatgat tgatcaaatg agtaaaaatc gtggcggaaa aactggtcca gatatcagca
     5101 acggtccggg aaaattagtg gaagcattag caattcctca agaactttat ggtcagtcta
     5161 tagcagatag ttcgttacgc ttagtttttg agaaaaaaaa gacaccaaaa aagatcaatg
     5221 ctttgcctag aatcggtatc ccaaacaaag gtgtatggac agaaaagccg ttgcgttttg
     5281 ttgtgtcggg aaatccttat atctctttac aaagaaaaaa ccaaatcgag aaagattggg
     5341 gttggaggaa agaaaatgaa aaagaaggat ctattaacat atttagatga aataattgaa
     5401 aaacaagtga cagactatga tgtagcttta gattgggata caaaaaatca tacgatcgag
     5461 atcgttgttc gcttgtttgc agaaaataaa gcacaatttg agattgatga tgcagaaggg
     5521 atcgtttcgc aagaaccaat tatcgaattt gaagacggcg tacttctttt taatcctcag
     5581 aaatctgttt ttgatgaacg caattatcta gcagtaatcc cttatgaagg aaaaaaaggg
     5641 ttggcaaaat cagtagctga ttctttagtg gaatatctaa atgaagtatt agctcaaggg
     5701 caaagcgatt tgcttgattt tttggatgaa gacgatgacg aagctgtatt cgagcttcac
     5761 tgggataatc agcaactagc agaattagta gaagtaaaac agcaaaatga agcagatact
     5821 tatttaccgt acccaagtta ttaaggagtg acagatatga aatggaatga agtaaaagta
     5881 gaaactgaaa gtgaagcagt cgaagcagtt gctaatattt tgatggaggc aggggcgagt
     5941 ggtgtcgcga ttgaagattc tttagatgtt gaacattttc agtcggatca atatggtgag
     6001 attttagata aaaatgattt tacatcgctg aaagaaggcg cactagtcat ggcttatttt
     6061 ccggaaacga ctttcttacc ggaaatcttg ccttttatca aagatcgtat cacacaatta
     6121 cctgagtttg gtctagctat tggaaaaaat aaagtaacag ttagtgaagt agaggaaagc
     6181 aactgggcaa cagcctggaa gaaatactat catccggtgc gtattacgcg aatgctgacg
     6241 gttgcgccaa gctgggaaaa ctaccagaca gaagatcctc tagaaaaaat tatcacacta
     6301 gatcctggta tggcatttgg cacaggcact catcccacga cttccttgac attgacagca
     6361 ctagaaacag tcttgcgtgg tggtgaaaca ttgctagatg tgggaactgg gtcgggcgtg
     6421 ctaagtattg cagcaaaata tcttggtgca aaagatgtct atgcctttga tttagacgaa
     6481 gtagccgttc gttcggcgaa agaaaatatg gatatgaatg aaatagccaa agatgtccat
     6541 gtatcagcta acgatctttt gacaggaatc gagattgaaa gtgatgtaat cgttgcgaac
     6601 attttagcag atatcatctt attgatgatc ccagatgctt ggcgtttact gaagcaaaca
     6661 ggcacgctga tcgtttcagg aatcattgaa gagaagaagc aaatggttct ggatgccatg
     6721 ttagaacaag ggtttatcgt ggatcaaatt ttccagcaaa aagactggta tgcaattatt
     6781 ttgaaaaaac cagaggtgga atagatgcag cggtattttt taaaagaatc atatgaatca
     6841 aaagactatt ttgttttaga aggggaaaac tatcaccaca ttgtacgggt tatgaggatg
     6901 gaacccgagg atgaagtttt tttggcattc aatgatcaaa tagccattgt tgcagaaatc
     6961 acggagatta ctgaagaggc tgtttatctg aaagaaatta aaaaagaaga aaatcaaaag
     7021 gaactgccaa gttcgattac catagcgagt ggctatccta aaggggaaaa gctggattgg
     7081 atcgttcaga aatccactga attaggagcg caagcttttc ttggtttccc ggcatcagcc
     7141 tctgtcgtta aatgggatgc aaaaaaacgt atgaaacgac aacaacggct tgaaaagatt
     7201 gctcaagagg cagcggaaca atcccatcgg cagatcgtgc catcagtaga gttattggaa
     7261 acgaaacaag atttatttga gcggctagat gattatgacg tggtcttggt tgcttatgag
     7321 gaatcagcaa agcaaggcga aaaatcaaat gcagccaaaa ttcttagtga aacaaaacca
     7381 gatagtcgaa tcttagctgt ctttggacca gaaggtggat tatcccgcga tgaagtcgat
     7441 tattttgtag aacacaaagg gatactttgt ggattaggac caagaatcct tcgtacagaa
     7501 acagcacctc tttatttatt aagtgctgct agctatcaat gggaattaat gaatccttct
     7561 ttcagctaac gttcttcatt aataaaagag tatcgacaga aagaattgaa aaaatagtca
     7621 acaatttgta taatagaaac aattatttat acaatggata cgaaaaatcg tttgaaaaag
     7681 cttttaaaaa gcgaacattt ccgtaaaaaa acgtataact gcacggattt tgggacaatt
     7741 atcaaacaga tatttgtccc gattcccgtt ttaccgaaaa aagaaagggg gatcgattcc
     7801 tgtgacaaaa gaagaaattt taacgggacc cggtgtcata aagctcgtta gtcaatacat
     7861 gggtcccgaa catgtagcat ttgttgagaa agcatgcgaa tacgcgactg ctgcacatga
     7921 cggacagttt agaaaatcag gcgaacccta cattattcat cctatccaag tcgcaggtat
     7981 attagcagat ttaaaaatgg atccccatac agtggctaca ggcttcttac atgatgttgt
     8041 tgaagataca gaaatcactt tagaagatct gagagaagaa tttggcgatg acgttgctat
     8101 gttagtggac ggcgtaacca aattagggaa aataaaatat aaatcccacg aagagcagct
     8161 agcagaaaac caccgaaaga tgctgctagc aatggctcaa gatttacgag ttatcatggt
     8221 caaattagct gatagactac ataacatgcg tacgttgaag cacctgcgag aagataagca
     8281 aagaaggatc gctcaggaaa ctttagaaat ctatgcgcct cttgcacatc gtctagggat
     8341 cagccggatc aaatgggaat tagaagatac agcacttcgt tatctaaatc caaaacagta
     8401 ttaccgtatc gtccacttga tgcagacgaa gagagaagaa cgtgaaaaat acgtaagtgg
     8461 tactgttgaa gatattcgaa tagcgacaga agagctgggg atttttgcag aaatctatgg
     8521 acggccaaaa cacatttatt caatctatcg taaaatgaaa gatcagaaaa agcagttcaa
     8581 cgaaatttat gacttgctag ctattcgagt gatcgtagat tcaatcaagg attgttatgc
     8641 tgtattagga gcaatccaca caaaatggaa accaatgccc ggcagattta aggattatat
     8701 tgccatgcca aaagctaata tgtaccaatc tttgcatact accgtgatcg gtccggcagg
     8761 aaatccggta gaaattcaaa tccgaacaca ggaaatgcat gaaatcgctg aattcggggt
     8821 tgctgcacac tgggcctata aagaaggaaa aaatgaaaaa gtagaaccag atggtatgac
     8881 gaaacaatta agctggttcc atgagatact cgaacttcaa gacgaaagct atgatgcttc
     8941 tgaatttatg gaaggcgtaa aaggagatat ctttagtgat aaagtctacg tcttcacacc
     9001 aaaaggagac gttactgagt taccaaaagg atccggacca ttggactttg catacagtat
     9061 ccatacagat atcggtaaca aaaccactgg tgcaaaagta aatggcaaaa tggtgcagct
     9121 tgattacaaa ttgaaaaacg gagatatcat tgagatcatg acttctccaa attcatttgg
     9181 cccaagtcgc gactggttga aattagttgc tactagcaaa gcaagaaata agatcaaacg
     9241 tttcttcaaa gcccaagatc gagaagaaaa tgtgatcaaa ggccacgaat ccgtggtcaa
     9301 atgtattaca gatctaggat ttacgcctaa agatattttg acgaagaaca aactgcaaga
     9361 agcactcgat cgttttaatt atcaaacaga agatgatctc tatgcagctg tagggtatgg
     9421 agaagttagc cccttgacga tggccaatcg tctgactgaa aaagaacgta aagaacaaaa
     9481 aatcgagcag caaaagcaag aagcagaaga aatcatgaat cagccgaaaa aagaacctga
     9541 caaaatgaaa gtacgtcatg aaggtggcgt tgtcattcaa ggggtagaaa acttactaat
     9601 tcgtatcagt cgctgctgta atccgattcc tggtgatgat atcgttggtt atatcactaa
     9661 aggcagaggg atatccattc atcgtcgaga ttgtccgaat gttcagcctg acaaaccaaa
     9721 tgtagcagaa cgtttgattg aagtcgaatg ggaagataca tcgaatacac gaaaagagta
     9781 tgatgcagat ttggaaattt acggctataa tcgttcaggc ttattgaatg atgtacttca
     9841 aacagtcaat gcgctaacga aaaatctcaa cagcgttgaa gcacggacga ataaagataa
     9901 aatggcgacg atccatttga cggttggtat ccagaattta tcccacctaa agagtatcgt
     9961 ggataaaatc aaagcagtac ctgatgtcta cagtgtacgc cggacgaatg gataggagga
    10021 cagatatgag agcagttgtt caacgggtaa gtaaagctgc tgtgtcagtc gatcagcaag
    10081 aaattggaaa aattggctac ggcctacttg ttttattagg cattcatgaa accgatacgc
    10141 aaaaagatgt ggactatctg gtaaaaaaaa ttgcacaaat gcgtattttt gaagatgaac
    10201 aaggaaaaat gaatattagt gtagaagatg tgggaggaca aattttaagt atctctcaat
    10261 tcacactgtt tgcggatacg aaaaaaggaa atcgaccaag ctttgtggaa gccgcacgac
    10321 cagaaacagc tattccttta tacgaatcat tcaatgaagg aatcagaagt cgcggcatca
    10381 cagtcgctac cggacaattc ggagcagata tgtcggtttc tctcgtcaat gatggacctg
    10441 taacgatcat tattgattct aaaaacaaat aaagtaatct ggttttcact agtaaaaact
    10501 atgaaacacg tcgcttaagt tagactgata aactctaact taagtggctt ttttgtctgc
    10561 aaaaagcgct tcttttgtca gcagtcggaa aaaaagttaa gcttaaatta tacaagttat
    10621 tttttatagg aggttgctga agatgaaaga agtaccagta gtagatatca aaatcacaga
    10681 tttaaaaaaa ttgtatcaaa aaggatacaa catgttggag gagttacgcc atgaagcgga
    10741 cgctcccgtc gtcaaagcaa agattttcaa taaagaagct atcacgatct acggatcatc
    10801 agcagcaaaa gtattttatg atccaaggaa tttcaaacga aaaggtgcta tgccaaaatt
    10861 agtattgaag acactttttg gtcaaggtgg agtacagact ttggatggag ctgctcatca
    10921 ccatcggaaa aatattttta tggatctaat gacaccggaa agaatggaag attatcatcg
    10981 tatcttagat aagaatctga cacaagcact tgaagcacag catggtcaat ttgaactatt
    11041 tgatttaagt aagatggttt tctttacatc tatttgcgag tgggcaggta tcaatttatc
    11101 agcgatttct aaagatgagg tcgaaaaatt agcagaatat caaatctcta tgatcagcgg
    11161 aacgtttact tctccgattg atcacataaa aggtgtcgaa aatcgcaaaa aatcagaaaa
    11221 atgggcgcaa ggcttgattg aagaagctcg acaaaatcct gtggctggaa aagaaaatgt
    11281 ggcattatat gcttttgcca atgccacaga tttagatggt cagcttttac ctctggaagt
    11341 ggcagctgtt gaattattga acatcatccg tccaacggtt gctctaactg tgtgggcggc
    11401 attgatggga catgcacttt tcagtagacc agatttgtat cagcaattaa aaaatgactt
    11461 ttcgacgtta caagatccgt ttattcaaga aatgagacgc tattatcctt tcttcccaat
    11521 gctacctgcg atttcgctga aagaagtaga agtagatggt tatcgtatac cagaaggcag
    11581 ttgggttatt ttagatttat atggaacaga ccatgatgaa cgaacagttg aagcgccaga
    11641 ttcctttatg atcaaacggt atgtagggaa agcaaaagat atttcctata aagaagaata
    11701 tgagatgatt gcacaaggcg ggggaaattt tcgacaaatg catcgctgtg caggagaatg
    11761 gatcactctt catagtctac gtgtgttttc cgatcagcta gtaaataagt ttgagttctc
    11821 tgtacctgaa caagattgga cgattccatt caatcagttt ccgacttatc cgaatagccg
    11881 agcgctactt tacaagaatt agattagcat aaacttatcg aaaaaaagga agatcaaaca
    11941 gtgctgatct tccttttttc atattactat aaattgataa ggtcaattga tcaaaatcca
    12001 cgaaatgctt catcaatggt ttgataatct tcattggata aatgcaaatc taaagcttgg
    12061 acattgtcag atacttgctc tggacgctta gctccaggta taactacaga cactcgtgga
    12121 ttcttcatgt accaagctaa aacaagttgc gcaggagtgg cttggtatct tttagcaatg
    12181 gggcgaagta catcgacagc ggttaaaatt tctctgaaac gttctccttg gaaatctgga
    12241 tttctttttc gaggatctcc ttcaccaaat tgtttttcct caccaagttc atattttcct
    12301 gtcagcaaac cggaagcaag aggaaaatag ggaacaaaac tgattttgtt tttttcaaga
    12361 taagggaata attctttttc agcctgccga tggatcaaac tgtatttatc ttctactaca
    12421 tcgacatatc catctgcatt cgcttctttc aattgttcta acgtgaagtt tgacacaccg
    12481 actgctcgga tttttcctgc ttcttttaat tcgtgcaatg tagcaactga ttcatttttt
    12541 ggtgtgcttt catccggaaa gtggatataa aaaatgtcga tgtaatcagt ttgcagacgt
    12601 tttagtgcat cttccaccga ttgtttgaga aactcaggag aattatcaaa tgttcgccct
    12661 ttatttggta catgagcagc ttttgtagcg atgacgacac gtgagcgatc atattctttt
    12721 tccttcagta cctcgccaat cagttcttcg gaacggccgt ttccgtaagc ataagcggta
    12781 tcgatcaaat tgatcccgct attcaaagct gtacgaacta cttgttttcc tgtctcatct
    12841 tccaaaccgt caaaaagatt atgtcctcca acggcatttg ctcctaatcc aagctgttct
    12901 gcgtagactt gtgaatgtcc gattcttatt tctgccatag ctgattcctc ctttttgtct
    12961 agtctaaaac aaaataccaa aaagctgaac taaaacgttt ttgtgagggg aaatagtcag
    13021 atatctcttg actttgagac tggtttttag taggttataa gtaatatcga atgtcaatga
    13081 aagagcgagt aagtttttga aactttacaa gagaggatca tcgaggctga aaatgatcta
    13141 aagtttcatt agcctaaaga cactctggag atggacgagt gaaaagccgt cccgcgtacg
    13201 agcgttattc gtaagaggaa agagaaatct ttcaaaatta ggtggtaccg tgtatgaatt
    13261 tcattcaccc ttgttcatga tgaacaaggg tgattttttt atttacatag ctaagaaagg
    13321 gagagaaaac tatgagtttc caaagaccaa aaggaacaaa tgatattttg ccaggcgaat
    13381 cagaaaaatg gcagtttgtc gaagaaacag cacgtttatt attcagagat tatcagtata
    13441 atgaaatgcg tacaccgatt tttgaacact atgaagtgat atcccgaagt gtaggggata
    13501 caacggatat cgtttcaaaa gagatgtatg atttttatga caaaggggaa cgccatgtga
    13561 cgcttcgacc agaaggtaca gcaccaatcg ttcgttcttt tgttgagcat aaattgttcg
    13621 gcccagagta tgcgaaaccg ttcaaaactt tttatatggg accaatgttt cgctacgaac
    13681 gccctcaaaa aggacggtta cgtcaattcc atcaaatcgg agtagaagca tttggatcag
    13741 aaaaccctgc aactgatgtg gagagcatgg tgatggcact tgatttcttc aaacagttgg
    13801 ggatcacgca aatccggcta gtcatcaatt cattaggtga taaagaaacg cgtcaagcct
    13861 atcgtcaagc attgatcgac tatcttttac cttttgaatc agagctgagt gaagactcta
    13921 aacgccgcct tcatgaaaat ccattacgcg tattggacag taaagacaaa cgagatcaaa
    13981 aatttgtggc agaagctcca tctatcttag attatctaag cgagcctgct caaaaacatt
    14041 ttgataccgt tgtttccatg ctggatgcat tggctgttcc ttatgaaatc gacagtaata
    14101 tggttcgcgg actagattat tacacacata cgatttttga aatcatgagt gacgcaccaa
    14161 aaatgggggc acaagcaacg atttgcgcag gcggacgtta tgacaatcta gtggaagaac
    14221 taggtggtcc ttcaacacct ggcttcggat ttgcaatggg tatcgagcga ttactcttga
    14281 ctatggaggc agaggaagta gttatccctg catttaatga gttggatgct tacgtggtcg
    14341 cactcggaga cgaaacaaat atcgaagcat tgaaagtggt ccaagcgatt cgaaactttg
    14401 gtttttcagc tgaccgcgac ttcatgaacc gtaaagcaaa agcgcaattt aagacagcag
    14461 ataaagcgaa tgccaaatta gtattggtga tcggtgaaga tgagcttgct agtcaaacga
    14521 ttaatgtcaa agcaatggca agccgaaaag aaaaaagttt tccattagaa gagatatatg
    14581 aacattttga tgaagtctat gatgaaatga ctttattcga gtaggaggaa aaaaataaat
    14641 ggcacaaaga acgatctatt gcggattagt ttccgaacaa caattagaac aaacagttac
    14701 attaaaagga tgggtacaaa aaagacgaga ccttggggga gtaatcttta tcgacctacg
    14761 tgatcgtgaa ggaatcgttc aaatcgtctt taatcctgaa atcaacaaag aagcatggga
    14821 aatcgcggat aaatgtcgca gtgaatacgt gatcgaagtg actggggaag ttagaagacg
    14881 cggagaaaat gcagtaaatc ctaaaatggc cactggtgaa ttcgaagtga ttgcaagtga
    14941 aatcgtgatt ttgaatcaag caaaaacccc gccatttcaa attgaagatg atcaagcaat
    15001 cagtgacgaa atacgtttga aatatcgtta tttggatctg cgtcgtccaa aaatgactaa
    15061 aaacatcaaa ttaagaaatg cgacaacaaa agcgattcgt cattatttag atgataatga
    15121 ctttctcgat atcgaaacac cttacctagg gaaatctaca ccagaaggcg caagagacta
    15181 cctagttcct tcacgtgtcc atgctggaca tttctatgcg ttgccgcaat ctcctcaatt
    15241 gtttaaacaa ttgctgatga atgctggatt cgaccgttat tatcaaatcg ttcgttgttt
    15301 ccgcgatgaa gatttacgtg gagaccgtca gcctgaattt acgcaagtag atattgaaac
    15361 aacgttctta acagcagaag aaatccaaac ttacacagag ggattgattg cgaaggtaat
    15421 gaaagaagtt cgcggtatcg aagtcacatt gccatttcct cgtatgacct atgatgaagc
    15481 aatggctcgc tacggcagcg acaaaccgga tacacgtttt gatatggaat tgatcgatct
    15541 aagcgataca gtcaaagaag tagaattcaa agtcttccaa atggctcttg aaaacggcgg
    15601 tgtcgtgaaa gcattgaatg ctaaaggcgc tgcagatcgc tactcacgaa aagacatgga
    15661 tcagttaggt caatatgtag gacaattcgg agctaagggg cttgcatggc tgaaagtaga
    15721 agaagacgga ttgaaaggtc caattgctaa attcatggga gaagcaacag aagcaatcat
    15781 taaagcaaca gatgcaaaac caggtgactt gctgatgttt ggcgcagata aaagtgaaat
    15841 cgtcgctgct gcattaggtg ctatccggac acgtttagga aaagaattag gactaattga
    15901 tgaatcgaaa ttcaatttct tatgggtaac tgattggcca caatttgaat tttcagaaga
    15961 agaaggtcga tatgtctctg ctcaccatcc attcacgatg ccaaaagaag aagatatccc
    16021 gttattggct gaagaccctg caaaagtcta tgcagaagct tatgatatcg tcttgaacgg
    16081 ttatgaacta ggcggaggtt ctatccgtat tcatactcgt gagcttcaag aaaaagtact
    16141 agaaactcta ggctttacaa aagaacaaat ggaagaacaa tttggtttct tgttaacagc
    16201 tttagattac ggattccctc cacatggcgg tattgcatta ggattggacc gttttgcaat
    16261 gttattagcc ggagaagata atattcgtga agtcattgcc tttcctaaaa acggaaaagc
    16321 agcagatgtg atgagtgatg caccaagtat ggtttcgcct ttacaattgt tcgaattgaa
    16381 tattgatgta acgaatatag aagaatagcc gataaaaagg taaaaaggat ttgtcgaata
    16441 cgcttctttt gacaaccttg atttaattga aaaacacttt ataatgaagc cataacatat
    16501 aaagcggggc ttttccatga aaaaaacatt cgaaaattgg gcttatcatg attacacgac
    16561 caagctttcg gtatctatcg tttatgcgtt gatcgcatca gtcgcattga atttctttta
    16621 tcagccagga catatttatt caagtggtat tactggtcta gctcagattt tgacgacttt
    16681 gtcaaaatca ctgataggat tcgaagtacc ggtatcaaca actttgtatt tactgaatat
    16741 acctttattt gtattggcgt ggttaaaaat cgggaagaag tttacaattt tcacttttat
    16801 cacagtcact ttgacctccc tttgtatcca tattttaccg gaaacagtcc tatctaccga
    16861 tccaatcatc tgtgcgttgt ttgggggggc tgtgatggga ctaggtattg ggctagcgtt
    16921 gagaaacggc ctatcatcgg gcggtctaga tatcgtgagt atcacgattc gtaaaaaaac
    16981 aggaaagtcg attggttcta tttcgatcta cttcaatgca ttgatcgttt ttgttgccgg
    17041 aattctattt ggctggcaat atatgtttta cagtacgttg tctattttcg tcagtggaaa
    17101 agtaactgat gtcgtttata ctaaacaaaa gaagatgcag gtaatgatcg taacgaaaca
    17161 tccagaaaaa gtagtaaatg aaatccaaaa ctgtatgcgt agagggatca cgatcatcaa
    17221 tgaagcagaa ggtgcttata agcatgataa gcagacagta ttgatgacga tcgttacacg
    17281 atacgaactg cctctactta gagaagcaat gaaagcttct gatccacaag cttttgtaag
    17341 tattgcagaa aacgtccaga tacttggtcg tttctatgag gatggcattt agatcgagtt
    17401 tgacaaaaat tcatgaaata ctgctgtaga cagacgttcg tttgtctgca gtttttttct
    17461 accatagtag aatcagcaaa gaaaaactgc ttttttattt gaaattttct taaagttgaa
    17521 aaaaagcatg tttatttttt taaatttatg ggatgataga aaaaaatagt agagaaagga
    17581 cacgattcta cttgctattt tcacttaata cccgtatatt acgaatgaaa aaagtttatg
    17641 aaaagttgga atattgaatg gaaaaacaaa aaaggttaaa aaacagccgc tacatcactg
    17701 ggtttgatgg gctccgttca ttagctgtgg tgggtgttat cttctatcac cttctgccaa
    17761 ctagtatgaa aggcggctat ttgggcgttc cgattttttt cgtcgtatct ggttatttga
    17821 tcacggatct gctgcggcag gaatgggaac aaaatggaaa aatcaatatt tggcaatttt
    17881 atgtcagaag aatgaagaga ctctatccgg cacttgtttt tatgcttgtg acagcttcgg
    17941 cttatatcac tttgtttcag cgagggctgt taaacaatct tcgaggagtt gtggtcagca
    18001 gcttactgta tgtcaacaac tggtggcaaa tcaataacgg tttatcttat ttcgatcgat
    18061 ttgctaatga gtcaccgttt acgcatatct ggtctttagc agtagaaggt caaaactatt
    18121 tggtttggcc gatcttgttt gtattgttga tggtatttgt tcgtaaaaga aaatggatct
    18181 tttatactgt gttaggtaca tccttcctct ccgccatctt gatgatgatc ttgtatacac
    18241 caggaggcga tccaacccgt gtttattacg gtaccgacac tcgtctgttt tctatatggt
    18301 tgggaagtgc gcttgcgttt gtttggccaa gtacgcgttt aaagaaaaac atcccgaaac
    18361 aagcgaaaaa tattctgaat atcgctggag gaatctcgct tttggcactg attctttgct
    18421 tcttcttttt agatgatcat tacaatttca tctattacgg aggaatgctc ctagtgagtc
    18481 tgttatgcgt agtgctagtt gcagtgacag cacaccccgg agcaagtttg aatcgatggc
    18541 tgacaaatcc agtatttact tggattggca aacgaagcta tggcatttat ctgtatcagt
    18601 ttccagttat gattttttat gaagcaaagg tgactgatat tgccgatcat gttttgttgc
    18661 atactttagt ggaaatcaca ttgattctaa tcatcagtga actttcttat cgatatgtgg
    18721 agcgtccgct agcacgtttt gattatggtc gtacattcca agtgatgaaa ggatggttca
    18781 gcaaaccgat catcagtaag agcaaaccat gggttgttcc aggcacatta cttgtagcaa
    18841 ttgcactcgt tggtttcgtt acggcaccga aaaacgctgt aacagccgat caaaaacaac
    18901 tgcaggcaaa aatcgctgaa agtaaaaaat tagcagaaga aaccagaaac aattcagaaa
    18961 acaccactag ttcggttgac caagcgatcc ttgataagta cgagttgtcc aaggcacaag
    19021 ggaataaggc acaaaaatta gaactaacgg catttggtga ttcagttatg ttggatgctg
    19081 caacggatct acaagaactt tatccaaaag tagtggtaga tggagatgtc ggacgacaat
    19141 tatatgccag tgtaccgtac attgaaaaat taaaggaaca agatttgttg aaagacactg
    19201 ttctgatcgg actaggaacg aatggctcat tctcggaagc tcagtttgat agtgtgatgg
    19261 atgcaatcgg tgatcgaaaa gtatactggg tcaatgtccg agtaccgacg aagcgctggc
    19321 aaaatgacgt gaatgcgatg cttgaacaga tgacgaaaaa atacgacaat atgacgctga
    19381 ttgattggta cagctacagt aatgaccata gtgactggtt ttatgatgat caagtccatc
    19441 caaatcctga agggatgaca cattacattc acttagtcag cgaagccttg ctaggaggaa
    19501 gttcaagtac gaacagcagt gagacaaacg tgacagaatg atagctacaa aaaacttcag
    19561 gcaaaaacat ttgtttttgc ctgaagtttt tttatattta ccgtgttttt aacattcata
    19621 tagattcagg aagcaaaacg gttgagtgtg tctgtaaata acgttctttt tgttttcggg
    19681 tgagtttttt gaatgcagct tctgcttttg ttgcttcgct tcttgaagaa aatacttctg
    19741 catggatcat cttaacaggt cttctttttt gaagacgagt atatttagca ccagtaccat
    19801 tgttatgttc ctctaatcgt ctagaaagat cggttgtata tccaccataa aatgtctgat
    19861 ctttacagaa taatacgtaa aaataatgtt catttgccat agagcatctc acgaatctct
    19921 gcagtgtatt ctccatccat attgtaagtg atcaatggtg gagcgacttt gaatccgtct
    19981 aatttgcctt ctttgattcc ttcaactaac aaaatatttg cctcttttcc aggttttggg
    20041 taaacaaatt ggatcctttt aggtgcaatt cgatagtctt ccatgacctg aatgatttct
    20101 aagaaccgat caggacgatg gaccatagta aagcgtccat ttgtttttag tagcttactt
    20161 gaaatatcga tcacttcttc taacgtcgta tgaatctcat gacgggcgat tgccaggtgt
    20221 ttattagggt tcgttttatt tgttggcagc cctttgaaat aaggtggatt acagacgact
    20281 aagtcacaag aattatgtcg aacgacagat aagctgtctt tcagatcgat ggtgtggaca
    20341 gtcatttgtt catttaaatg gttaagcgcg atactacgtc ttgccatgtc tgctagacga
    20401 ggttgaagtt cgatagcgtc gatagccgcc tgggttcgtt tacttaaaaa aagtccaact
    20461 gcgccatttc ctgcacaaag gtcaacaatc ttcccttttt taggtacacg ggaaaagtga
    20521 gcaagcagca cagcatctaa agaaaaagaa aacactttgg aactttggat aatcttgata
    20581 tcttctgcgt ataattggtc gattcgttcg tcttcgtgaa gcatggattc cctccaaaat
    20641 gaaaaaaata gtcgtctgta ttatactatt tttattaaaa agaaccaagg tggcttgtca
    20701 aacctctatt ttttatgcat actatgacca taagaaggga aaggaagaga ctatgtttta
    20761 tcgatttatg cgcggggtag ttcgagtact attagcctta gttaatggaa atgcccgcta
    20821 tgagaataaa gaagttttac cgcaagatga gaattacata ctagttgctc cccatcgtac
    20881 ttggtgggac ccattatatc tggcggtagc tgcagcaccc aaggaattca gctttatggc
    20941 aaaagaagaa ctgttcaaaa atccagttct gcgatttata ttaactcgat ccaatgcttt
    21001 tccagttaaa agagaaaatc caggaccaag cgtcattaaa acacctgtaa agattttgaa
    21061 atcgacaaat ttgagtttga tcatgtttcc cagtgggaca agacatgcta cagaacttaa
    21121 aggcggaatg gcattgattt ccagaatgtc taaagcgaaa atcgtgccag ctgtatatca
    21181 aggtccgttg acacttgggg atctgttcaa gcgtcagaga gtgacagtgc gtttcggcga
    21241 accaatcgac ttatcagata tcaagaaaac ggataaagaa ggtatggcgg agatcgaacg
    21301 acgaacacaa gcagcttttg acaaattgga caaagaagtg aaccctgatt tcaaatatga
    21361 agtcaaaaaa aaataaaatg ataagtttat gcgtgggcgg tcttttgaat ctactgtcct
    21421 gttcagttca gactttttca atgaaaacga gggagagacg tcaagtctgc cctctttttg
    21481 cgttccaata tgtaaaatag aagacaggtg tcatgtaatg gatcaaagtg ttataataat
    21541 tagaaggaca ttttttgagg tgattgaatg cgttttttac atacagccga ttggcatatc
    21601 ggaaaaaaac tacaaggtta tgacttgatt gaagaccagc gtcatgtact atcttctata
    21661 ctagatatcg cgaaagaaga aaaagtagag gcaatcgtga ttgccggaga cttatatgat
    21721 aggagtgtcc cagctgttga tgccgtagaa ttatacaatg aattgatggt cgattggaat
    21781 ttaaaggaaa agatcccgat ttttgctata tcaggaaatc acgatagcag tacgagactt
    21841 tcagcgggta cgccatggtt cagttattcg aactattttt tgcgaacaca gctagcagaa
    21901 gcatttgaac cagtcgagtt taaaaacgta caatttttct ttcttcctta ctttgaacca
    21961 attgcagcga gattatattt tgaagatgac aatatccgaa cgatcaagca agcggtagcc
    22021 aaggtgatag ataaaatgca ggagctattt gattcagaaa aaaaacaagt attgattagc
    22081 catttttttg taatgggaag tgtaaaatct gattcggaaa cgaaaatcga agtaggcggg
    22141 ttggattcga tccctggtga actacttaca ccgtttgatt atgtagcact aggacatttg
    22201 cataatcaag ctgctttgag acaggataat gctcgctata gcggttcccc attgaaattc
    22261 tctttatctg agatcaacca aaaaaaaggc gtatggatcg ttgacctgac aacaggcctg
    22321 gagttgaatt ttagggagat cgaacccctg catgatattg aagaaataac agcgagtttc
    22381 aaagaattgc tggatcctgc cttttatcag tccgtcaatc gggagaatta catctatatc
    22441 tatctcactg atagaacgat catcccgaat atgatgaacc agcttcggca aatatatcct
    22501 cgtattttgg ggattgaacg ggcaaacggt cgagcagaac aaacaaagcg caaacgaaaa
    22561 gtactagaaa tgaaagatcc tcaaaaatta gcagaagcat tttttagtga aatgacggaa
    22621 gagcagctaa ctgcaaacca agaaaaatgg ctcggagaaa cgttgcaaga aatcatggaa
    22681 atggaatgat caagaatgaa accgaaaaaa ttacaactta aaaattttgg tccttttatc
    22741 aatgagaccg tagatttttc acgtttgaca gaagcaccgc tttttttgat cagcgggaag
    22801 acaggagctg gcaaaacgac gatttttgat gggatcacct tcgcactttt tggtgaaact
    22861 tccggaagac ttcgctctgg aaaagagatg cgttctttgt ttgctacacc agacgaagaa
    22921 acaagcgtga ctttttcttt tgaacatcag cagatgagct atgaaatcga acgaaaacca
    22981 gaacaagttt tagcaaaacg aaaaggaaac ggaacaaaaa aacaggcagc caaagtaacg
    23041 ttgaccattt ttgatgggaa aggaaaagaa ataaaacaaa tcacaaaacg tacagaagca
    23101 gatcagttga tcagagaact gatgaatctc gatgcgaaac agttttctca aatcgtttta
    23161 ctaccccaag gagaattccg taattttttg gtttcatcaa gcagtgataa agagactgtt
    23221 ttacgtcatt tattcggtac tcaatttttc caacagttca atgagcgatt aaaagaaaaa
    23281 gcaaaacaac agcaacaaaa attggatcat ttagagcaag agctgcatct tctgcaaaaa
    23341 aggtttgttt tagtcaaaga agaggaacca gcagcagggt ttgaagccgt tttagcccaa
    23401 tggacaaacc atcaggcaat actcgaaaaa caaatcacgg aagaaaaaga agctctaacg
    23461 gaacttcagg aaaaacaaaa gcaactagaa aattcctact atcgttttga aagcttgaaa
    23521 aaaagttatg aagaaaaaac gatattaata aacaagcaaa aggaattagc tgctcaaaaa
    23581 gacgaaatag aggaaaagaa acgatggatt gggtattatg aatttacgca acgattagtc
    23641 gaaccaatcc agcgcctaca agaaataaaa gttgaaaaaa tagaactgtc acaaaaaagt
    23701 agtgagcaaa caaagaaact agcgatgact acgcagaatt atgaagagtg gcaaaaaaat
    23761 gaagcaaacc gcgtacgccg acaaacaaaa atcaaagaag ctgctcaaaa actgcaaaac
    23821 aaacagatga tggtgccaat tgtcgaatcc ttacgtagta aaaaacaaga cgcagaacaa
    23881 ttaacaattt cattggataa agaagaattg aatgccgaga agctgtatca aaatctcgct
    23941 atctatcagg aaagaactcg aattcttcaa gaaaatttac tggatcagga aaaactgcaa
    24001 gaagatcgtc ttgcttttgg acagttaaaa tatctagatg aaaagcagca agaaatagaa
    24061 aaacaaaaag caaaagacac tgggtcgctc gcacagataa gagaagaact caaaaaagca
    24121 actgaagaaa taacaaaaaa tagtcagcta ttgaaagaac agacagaaca agcagaacag
    24181 ctgaaaagcc aatgggcaaa gcagcaaatt gctcgtcttc agcttttttt gcgtccaggg
    24241 gagccatgtc ttgtttgtgg atcaacggaa caccccatac aacaattaaa acacgaacag
    24301 ccaaataagg aagatatctt aaaagtagaa caacaactgg cagaagctga gtctgctgta
    24361 gaaagaactc agcaaatcat tgccaaaagt gaagcacatg aaaataatct tcaacaacaa
    24421 ttatcagatt tagaacaaaa aatcatggca acgaatatac aattggctga acaagaagaa
    24481 cacttgcaac aggctttgaa aaaggcattt cctgagataa cagaacaaaa aacaaaaaca
    24541 actcttgtag taatcgaaca atctttagat agacgaaaaa gcaagatggc caatgcaaga
    24601 atagagctgg aagaaataag acaacaagca gaaaagctcg aacaacaatt agctgtctac
    24661 aaacagcagc agacagaaaa aaataatcag ctccagcaac tgcaaggaga aatcaaaagt
    24721 ttagaacaac aattgcaaga tacggatagt aactgtttag aagaggagat acgacgactt
    24781 gaaaaacagt tagaagaaga ctcgaatcaa ctagctgttg agcagaaagc aggagtgcaa
    24841 ttgttgcaag atcttcatac gcttcggaca cagcttgatc ttcaacatga acaaatagaa
    24901 aaaatagatg aaaaaataga aaaggaacaa gaaagaatag ctaaaaagtt agaaagccag
    24961 tcgtattttt caacaattga agaaatctgt gcatttgcac aacagcaagc acaatataaa
    25021 gaaaaccaac gtacagttac ttcttatcat gaagagcaat tagtgacagc tgaccggctg
    25081 cgtcaattag aaaatatcca gctaactgag acattcccag atacagaaga gatcagccaa
    25141 cagttagaag aaataagacg tatgatcgaa ataaagcaag aaaagatata cggtttacaa
    25201 gaacaaaaaa ttagtaatca aaaaatctac gatgaatgtc tttcgattta tcacacaagc
    25261 cagacgaaat tagatgagct tacgcaatta cagcagcttt cgcaaacatt caacggagaa
    25321 aatcctaaga agactagttt ggaacggtat gtgcttcagg tttatcttca agaagtgtta
    25381 caagtagcaa atgatcatct ccaacgttta acgaaaaatc gttatcaatt tgagctagcg
    25441 gataccattg gcagttatcg agggaaaacc ggattagaaa tcaatatcta tgatgatcag
    25501 gcaggtatgt ctcgaagtgc gcataccctg tctggcggtg agagttttat tgcagcactt
    25561 tctttagcat tagctcttgc cgatgtgatc cagacacaag ctggtggtgt agcaatcgat
    25621 gcattgttta ttgatgaagg atttgggtca ttagatgaag aagctttgga aatggcaatg
    25681 gaagctttag aaacgatcga aagtgaagga agaatgatcg gcatcattag tcatgtaaga
    25741 gaattgaaag aacgagtatt acaacaaata agaattgata cagaaggtag cggacaaagc
    25801 aaaatacgct atcaacttgg ataaaaaggg agattggtaa ctatgaaatg gagtattcca
    25861 gaaaaagtca ttgcacgtgg acgtcaatac ttgcaagaag gccgagtgtt atcggttgtc
    25921 cctgaccaag aagaaaacgt gtggcatgca gaagtcttgg gcagtgaatt gtatatggtt
    25981 gatttggatg gtacagcaaa agagatagat tactgccaat gtccctattg ggaggaacat
    26041 aaatattgta agcatacagt tgctgtggaa ctttatttac ggtcgaaaaa tctatctcgt
    26101 atcatgaaaa aagaccagcc aatgctagaa ccggtaaaag caacaagtga gggagaaatc
    26161 attgcaaaag gtttttctcg tctaaaaaca caaaacaatc ctcaagcggt gcaacctttg
    26221 attattgaat accaagtaga aacgattgag actaatcagt atcacccaga gctttctatt
    26281 ttggcacttt acttgcgtat cggttcattg gcatcaccga aaaaaacata tatcgtaaaa
    26341 aacatctatg attttttaca agcttacaca gaaaaagaaa cttacactgt taataaacag
    26401 taccagtttc gtctagataa acgggcgttt caaaaagaag atcaaaaaat actcacgcat
    26461 cttgcaggaa gtgctcagac gcatcaatta ttaggtacga acggcttaca agtcaaagga
    26521 aaactagata aaagatatct gcttctccca gtagaacaag cccgcttcct gttagagcag
    26581 atgaatcaga ctacacgttt gcatctgcag ataaatgaac aaaacgatca aggaatacaa
    26641 ttttccgatc aaaacaaacc tttatcattc aaagtggaaa aatttggaga agattatttg
    26701 ctgcaagcat tgaatgattt tgattttttc tttatgcatt atcagtgggg agttatccaa
    26761 gggacatttt attctctgac gaaatatcaa caaacggttt atcaaacttg gaaacaattg
    26821 attcgccgat tggagaaacc agaagtagtg ttcaagaaaa aagaattacc gtctttgttt
    26881 aaagagatta tccctctgct gtcagagatc ggcgacgtag ctattgcccc tgcagtagaa
    26941 gaggaagtag cagatatccc cgtcgaattt attttctttt tccgaaaagc aaaaggaaaa
    27001 atccaagctc gagtcgattt cgtctatggc gaagtcatct attcgactga cccgaagcat
    27061 gaggtctcga cagaacaggc ttttcaggtg ttacgtgaca cagtagaaga gaaacgagta
    27121 cttgatctat tcagaatgta tcgctaccag aaaaatgaaa ctggttatga acgtccattg
    27181 ccaagcgggg aagaactata tgcctttttc cgcacagaac tatctgtctt ccgtcagttt
    27241 ggtgaagtca gacttggaag aaaattgcga gagttgtatt tagatgcgca taaacatcaa
    27301 ccgaaaatcg aagtaaccga gaccggttca tggctcgata ttcgattcga tgtgacaggg
    27361 atagaggagc aagaaatcga tcatgtcctg caaagcttgc tgagaaatga tgctttttat
    27421 acattagaaa atggtcaggt tctttctttt gattcagaag aatttcaaca aacaagccaa
    27481 gtgttgcaac aattacggga atcgatacgt acagaagaag gaacgatcca tgtacctaaa
    27541 aatcaaggat tgatcattca aaatcaattg gaaaaaagca atgctacttt tagtgaatcg
    27601 ttccaaacga tggtacaaga tctcattcat ccagaaagat accaggcaca gcttcccaaa
    27661 ggattgaatg caacgatgag agattatcaa aagcaaggtt ttcgatggtt gaagatgtta
    27721 ggtcattatc agtttggcgg tattttagcc gatgaaatgg gacttgggaa aacactacag
    27781 acaattgcct ttttactttc ggaaaaagaa gaacggaaaa gcttctctgc tttgatcgta
    27841 gcgcctgcga gcttaattta caactggcag gcagaggtga gaaaattcgc cccttcttta
    27901 agtatccaag taatcaatgg gaataaaaaa gaacgtgaag aattactggc taaagacaca
    27961 gatatccgag tgacttctta tgccagctta agacaggatc tagctaatta tcagtcgcaa
    28021 aaaattgatt atttgattct tgatgaggca caaatggtca aaaatagcag tacgaaaact
    28081 gcacaagcat tgagagaatt agctgtacct cagcggtttg cactgagtgg gacaccaatc
    28141 gagaataatt tagaagaatt gtggtcattg tttgcaacga ttatgccagg atttttcccg
    28201 actaaaacaa aattcaggga acttgccgca gaagaaattg cgcaaatgat tcgaccgttt
    28261 attttacggc gagataaaca aactgtgttg aaagacttgc cggaaaaaac agagatgaat
    28321 ctatattctg cacttacaga agaacaaaaa actgtttatt tagcttactt gcgtcaaatg
    28381 cgtgaagaaa tcacatcaat ggattctgaa gcattcaaga aaaaccggat cgggatactt
    28441 gctggtctga caagactgcg acaaatttgc tgcgatcctc gtctgtttat tgaagattac
    28501 caaggcggtt ccggcaagct cgaacaagta aaagatctac tggtagctgc caaagagaac
    28561 aaacgaagag ttttgctgtt ttctcaattt acagggatgc tgactatctt gcaagaagag
    28621 ttagccgagc tagggatctc gacattttat ttaagaggca gtacgaaacc acaagatcgg
    28681 ttaagtatgg tagacgcatt taatgctggc gaaaaagatg tttttctgat ttccttaaag
    28741 gctgggggaa ctggattgaa tctcacggga gcagatacgg tcatcttgta tgacctttgg
    28801 tggaatcctg ccattgaaga acaagctgct ggacgtgcac atcggatcgg acaaaaaaat
    28861 gtggtagaag tgtggcgtat gatctctgaa ggaacgattg aggaacgcat ggattcactc
    28921 cagcaagaaa aacgcgaact gttccaaaaa gtgatccaag gtaatgaaga acagttgacg
    28981 aaactgactg aagaagacat ccgcatgatt ttaagtgtag gagaaatcga ggagtaagag
    29041 actcgaaaaa gcgttcagta atgggtaaga aagcctttct aaagaagcga ttgtttattt
    29101 gatttaaaaa atagcaagga ggctgggatt ccattttatc ccagcttcct tgctatcatt
    29161 tttttatttc tatttttaaa ggttatacaa agattgtctt cattgtctct aacagctgat
    29221 caaaagaaga aatctcgata tcgcaaattg gagtctgttc aattttgcgt tctcgatggt
    29281 tgaaccaaag cgcttgccag ttagcacttt ttgcacctag aacatcattg tcgtaattat
    29341 cgccaacgta taaggtctct tctggaagca tatgaaattc tttctcagct agctgaaaaa
    29401 tctctttttc tggtttttgg aaacctgttg cttgagaaat gagcatattg cgggaaggaa
    29461 tccaattgtt caattgaagt tgcttcagtt tttttgtctg atggtcagtc ggcccatttg
    29521 tgataattcc taatgggaca ttcttttcct tcaagaaatc gagggttttt ttgacctcct
    29581 catgcaagca gatattatca agttcttcct cgtaaatttt ttgaaataag aggccatctt
    29641 cttccgtaat atgagggtag tctaaatctt ttagtgattg gctgatccga tgtgctcgca
    29701 tatattctag tgtccaatct cctgccatta cttttgggaa gttttcatca ctatgatggc
    29761 ggaaccggat atatagtgga tgcatatctg catctgaaac aagcggaacg actcttttga
    29821 ccgcgtttcg aaatggctgc tgctgatcat acatcgtatc atcaacatca aacacaactg
    29881 atttaatcaa aattttacac gcctttcggt tttcattgat tttttcacaa atccttgact
    29941 attgtaacag aaaaaattgt ctaaaaaagc tgataaataa aggtttttct tactatattt
    30001 ctatttcctt gacagaataa ggtttttata tttcagtttt tttcactaaa aatcgtgaaa
    30061 cacgtcaata agtttgatga acaaattaat cattatggta gaagtcgatt gatttatttt
    30121 aatgcaatct ctgtggaatt ttcgaaag
//
LOCUS       DASJSZ010000028        29974 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.028, whole genome shotgun sequence.
ACCESSION   DASJSZ010000028 DASJSZ010000000
VERSION     DASJSZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.028"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(61..2424)
                     /locus_tag="U4B03_001183"
     CDS             complement(61..2424)
                     /locus_tag="U4B03_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEN2068050.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="U4B03_001184"
     CDS             2872..3093
                     /locus_tag="U4B03_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2068051.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="U4B03_001185"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="U4B03_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEN2068052.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="U4B03_001186"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="U4B03_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEN2068053.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="U4B03_001187"
     CDS             complement(5894..6205)
                     /locus_tag="U4B03_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEN2068054.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="U4B03_001188"
     CDS             complement(6202..6495)
                     /locus_tag="U4B03_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEN2068055.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="U4B03_001189"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="U4B03_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEN2068056.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="U4B03_001190"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="U4B03_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEN2068057.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="U4B03_001191"
     CDS             complement(8333..12685)
                     /locus_tag="U4B03_001191"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEN2068058.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="U4B03_001192"
     CDS             complement(12897..14606)
                     /locus_tag="U4B03_001192"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEN2068059.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="U4B03_001193"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="U4B03_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEN2068060.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="U4B03_001194"
     CDS             complement(16103..16903)
                     /locus_tag="U4B03_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEN2068061.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="U4B03_001195"
     CDS             complement(16900..17712)
                     /locus_tag="U4B03_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEN2068062.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="U4B03_001196"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="U4B03_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEN2068063.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /locus_tag="U4B03_001197"
     CDS             complement(18467..19189)
                     /locus_tag="U4B03_001197"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEN2068064.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /locus_tag="U4B03_001198"
     CDS             complement(19325..20206)
                     /locus_tag="U4B03_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEN2068065.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="U4B03_001199"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="U4B03_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEN2068066.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="U4B03_001200"
     CDS             21446..21925
                     /locus_tag="U4B03_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEN2068067.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="U4B03_001201"
                     /pseudo
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="U4B03_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            23226..24011
                     /locus_tag="U4B03_001202"
     CDS             23226..24011
                     /locus_tag="U4B03_001202"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN2068068.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /locus_tag="U4B03_001203"
     CDS             complement(24357..26048)
                     /locus_tag="U4B03_001203"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEN2068069.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="U4B03_001204"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="U4B03_001204"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEN2068070.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="U4B03_001205"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="U4B03_001205"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEN2068071.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="U4B03_001206"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="U4B03_001206"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEN2068072.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         7994 a         6451 c         5340 g        10189 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcata ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgta acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaa
//
LOCUS       DASJSZ010000029        29121 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.029, whole genome shotgun sequence.
ACCESSION   DASJSZ010000029 DASJSZ010000000
VERSION     DASJSZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.029"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            64..516
                     /locus_tag="U4B03_001207"
     CDS             64..516
                     /locus_tag="U4B03_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068073.1"
                     /translation="MTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRLLK
                     GDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSNLF
                     FGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            1104..2543
                     /locus_tag="U4B03_001208"
     CDS             1104..2543
                     /locus_tag="U4B03_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2068074.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2838..3056
                     /locus_tag="U4B03_001209"
                     /pseudo
     CDS             2838..3056
                     /locus_tag="U4B03_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3305..3520
                     /locus_tag="U4B03_001210"
     CDS             3305..3520
                     /locus_tag="U4B03_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068075.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3737..3993)
                     /locus_tag="U4B03_001211"
                     /pseudo
     CDS             complement(3737..3993)
                     /locus_tag="U4B03_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4180..5091)
                     /locus_tag="U4B03_001212"
     CDS             complement(4180..5091)
                     /locus_tag="U4B03_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2068076.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(5110..6114)
                     /locus_tag="U4B03_001213"
     CDS             complement(5110..6114)
                     /locus_tag="U4B03_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN2068077.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(6111..8234)
                     /locus_tag="U4B03_001214"
     CDS             complement(6111..8234)
                     /locus_tag="U4B03_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2068078.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(8239..10686)
                     /locus_tag="U4B03_001215"
     CDS             complement(8239..10686)
                     /locus_tag="U4B03_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2068079.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(10673..11062)
                     /locus_tag="U4B03_001216"
     CDS             complement(10673..11062)
                     /locus_tag="U4B03_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2068080.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(11122..11625)
                     /locus_tag="U4B03_001217"
     CDS             complement(11122..11625)
                     /locus_tag="U4B03_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN2068081.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(11638..11862)
                     /locus_tag="U4B03_001218"
     CDS             complement(11638..11862)
                     /locus_tag="U4B03_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068082.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(11966..13876)
                     /gene="ltrA"
                     /locus_tag="U4B03_001219"
     CDS             complement(11966..13876)
                     /gene="ltrA"
                     /locus_tag="U4B03_001219"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2068083.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(14622..14759)
                     /locus_tag="U4B03_001220"
     CDS             complement(14622..14759)
                     /locus_tag="U4B03_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN2068084.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(14756..15940)
                     /locus_tag="U4B03_001221"
     CDS             complement(14756..15940)
                     /locus_tag="U4B03_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN2068085.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(16199..16489)
                     /locus_tag="U4B03_001222"
     CDS             complement(16199..16489)
                     /locus_tag="U4B03_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068086.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(16479..17621)
                     /gene="dcm"
                     /locus_tag="U4B03_001223"
                     /pseudo
     CDS             complement(16479..17621)
                     /gene="dcm"
                     /locus_tag="U4B03_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(17697..17861)
                     /locus_tag="U4B03_001224"
     CDS             complement(17697..17861)
                     /locus_tag="U4B03_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068087.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(17854..18528)
                     /locus_tag="U4B03_001225"
     CDS             complement(17854..18528)
                     /locus_tag="U4B03_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2068088.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(18606..19946)
                     /locus_tag="U4B03_001226"
     CDS             complement(18606..19946)
                     /locus_tag="U4B03_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN2068089.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(20029..20364)
                     /locus_tag="U4B03_001227"
     CDS             complement(20029..20364)
                     /locus_tag="U4B03_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEN2068090.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(20374..20748)
                     /locus_tag="U4B03_001228"
     CDS             complement(20374..20748)
                     /locus_tag="U4B03_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2068091.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(20761..21075)
                     /locus_tag="U4B03_001229"
     CDS             complement(20761..21075)
                     /locus_tag="U4B03_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2068092.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(21188..24415)
                     /gene="fss3"
                     /locus_tag="U4B03_001230"
     CDS             complement(21188..24415)
                     /gene="fss3"
                     /locus_tag="U4B03_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN2068093.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            25203..25526
                     /locus_tag="U4B03_001231"
     CDS             25203..25526
                     /locus_tag="U4B03_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068094.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            25690..26607
                     /locus_tag="U4B03_001232"
     CDS             25690..26607
                     /locus_tag="U4B03_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2068095.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            26600..27382
                     /locus_tag="U4B03_001233"
     CDS             26600..27382
                     /locus_tag="U4B03_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068096.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            27383..28063
                     /locus_tag="U4B03_001234"
     CDS             27383..28063
                     /locus_tag="U4B03_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2068097.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            28056..28952
                     /locus_tag="U4B03_001235"
     CDS             28056..28952
                     /locus_tag="U4B03_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2068098.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
BASE COUNT         8409 a         6005 c         5231 g         9476 t
ORIGIN      
        1 ctatcaggta gaaccaaaag ttgtggatgt ggaaattata aaaataggca taaatataaa
       61 aatttgacaa ataaaacttt tggtaagtta gttgcaatca agccaactaa actgcgaagt
      121 gaaggaactg ttgtttggga atgtcaatgt ctgtgtggga gaaaaaagtt agtatcggca
      181 aagcgacttc taaaaggtga tgtaaaaagc tgtggatgtt tgaaatcaaa aaagcatcac
      241 attgaaaata aaaaattcgg attacttact gcaaagtatc ctttggacaa taactgttca
      301 tggaaaacaa aatgggtttg tcattgtgag tgtggcagta tgtgtgagat aagttatagt
      361 aacttatttt ttggacatac tcaaagttgt ggatgtttaa aaaggcaaga aacaaggaca
      421 tgtgttgaag ggacagtaat agagtgtcta acgtcaaaat taccgaaaaa taatacaagt
      481 ggtgttaagg gagtttatcg ttctaaagag aaatagattg cgtatataac tttaaaaaaa
      541 aagaggtact atttaggatc gtatgattct attaagagtg cagctcaagc tagaaaagat
      601 gctgaaaata aattgtttca acctttgatt gacaaatatc gaggtaatca ttaattattg
      661 tggataataa ttaacagtga tatataaaca tctataatat attattcata ctattgatat
      721 ttagtataat cctatggaat tgaatatcat agcaatgtat ttgaaaagca aattggaaaa
      781 caaataggtg caacaccata aatatgaaaa aattgggaaa aagtagaatg gaaatttgct
      841 tacgtctcac gagatagctc acactattaa tctgaagaac aatgaagtcg acttttagaa
      901 attgctagaa gaaagtaaag cgaattatca ctagttacta tagagtaagt tcaaataaag
      961 catctccaac ttacatggtt acatgaggat atcaaatcga aattactcaa gcttcctatg
     1021 gctagagtcg cgagaagttg ttgggcagta gacataggtt atattgttct tagctacacg
     1081 atcattagaa aggaaatcct attatgtata gttatcaaga agatgcaaaa gtccaaaaaa
     1141 atcgctggtg gattttgatt gctatatcta tgtttacctt catgtccaca ttggatggca
     1201 gtattgtcaa tattgcacta cctaccatct cagatgatat gggcatacca atgaatcaag
     1261 cggaatgggt agtgtccgtt tatctgatga ttgtctgttc ttgtttgttg ctgtttggta
     1321 aaattggcga tagccgtggg aaaatcaaag tatttcggat tggtacgctg atctttgtct
     1381 tgggatcgct gttgtgtggg ttcaatcatt cgctaggtat tttattgttc gcacgggtgg
     1441 tacaagccat tggtgctagt atgactatgg ctacaaatgc cggaatcatc acggaagtct
     1501 ttcccataca tgaacgtggt cgggcattgg gagcaatagg tgcgtttgtt tcattaggct
     1561 cgattgctgg accaggtctt ggcggcttga ttttgtccca attttcttgg gcgtacattt
     1621 tttggatcaa tgtaccggtg ggaatcgtaa cgatgttgat tgctgaaaaa ttcttaccaa
     1681 aagatatcac catgagcggc catacaatcg atggaggggg ctttgctgcc attgcggcta
     1741 cgattatcag cttttttgga gcaatctttt tggggcaaga aagtggcttt ttccatccag
     1801 tcccccttgt actgttcgta atttcagtga ttgcctttgt cagcttttta aaagttgaaa
     1861 agaaagcagc ctctccattg atttctttcg gtatttttaa gaacaagatt tttaccatga
     1921 gcttgattac agccttgttg attttttctt ccaacttctt tgtcaatgta gtgattcctt
     1981 tttatctgca aaatgctcgc gggttgagtc ctagctatgc tgggctgttg atgatggtct
     2041 ttcctttgat gatggtcgtc ggggcgcctt taagcggcta cctgaccgac cgggttggcc
     2101 cggagatttt ggtattagta ggcttgggct tattgtcggt aacccaggtc atgtatatgt
     2161 ttttgtcagt ggactctcct ttgtggctat acattgtcgc tactgccatc atgggtgccg
     2221 gcaatgcctt gttccagtcc cccaataata cgatggttat gagctcggtc actatcgaaa
     2281 acttgggact ggcaggctcg atgaattcct ttgcccgaaa tttggggatg gtcagcggga
     2341 tttcgctttc tacgacggtc ttatatcaag cgatgtctgc taagatgggc cagcaagtaa
     2401 ctaactatat cgcaaatcgg ccggatgtct ttttatatgg aatgaagatt acctttttag
     2461 gcagctttgt tttgtgtttt gttgcctttt tcctgactat ctggcgtatc aaaaaacaag
     2521 gaggtattca gaaaaatgtc tgaaaaaacc actgttgaag aatttttgga ggcctgcgta
     2581 tgggtgagtg ggatttccat ggatgatgga agatgaagtg aacttctaaa agccgaaaaa
     2641 ctcaagtatt atcttcaaaa tactagtgct caatgagatg cttgatttga tcacaagaag
     2701 actgcgaaag ccgctggcaa ccgcaagcta ggtatcccac cgatggacta cgttttagag
     2761 gctactggtt atgaatacgg agccaacgct ctcatctgca taagaataca tcacccagat
     2821 tatgtaaaga ttttcgatac ttctgtcaaa aaatcattaa gatagataaa tgatttccgt
     2881 ctagtcaaat tggttcagaa tgtagacaca cagatggtaa gaagtcactc acaattcgag
     2941 aatggacttg tcctatttgc cgtatacatc ataatcgaaa cgtcaacgat agtatcaata
     3001 ttttgaccaa gggctatgaa tacaagcatt gccttagaaa acatcaagaa ccttaggaac
     3061 aacggggata gcttggttaa taagagatac cgctgtttag gcttgcaagt atactctttt
     3121 atcaagaatt ccgtggcttt agtcatgaga ggcttaatac tatgaaaatt ttctttagca
     3181 tttttgattt aggttccttt tctaattata ataagatgac cactacagta acctactgaa
     3241 atatataaac agccactgta gtaccatttt caaagtagtc tatgagagga gaatcttcat
     3301 tcttatgaac gctggaattg cggatatgaa tttaataaaa aaaaccctta atgattttac
     3361 ttccaattca atatcaaaag gtactggtat caatttaagc acaataaaaa agctgaaaag
     3421 tggtgaaaga tcagtggaaa agctgaatct tttagatgct ataaaaataa cggaatttgc
     3481 tatgaaaaat ggaaaagctg agattgaaat atggagataa ttatcgtcta gtgaattact
     3541 tgccacttag ctgaatttaa cgatccttgg tgcttgaagg gggagattct tgggaataga
     3601 gcatacttgc aagcctatgt ctttactaac aacgatatct cttattaacc aagctatccc
     3661 cgtagttcta aggcttttac ttttatctaa gcaataatcg tattcaaagg aaatttttga
     3721 cccttagaaa gtctcatttt atcagacgtg agcttaggca ttgctgaatt ttgttaattc
     3781 ggttttaggt tgtgctttga gcataatatg aaagtattct ttatcacgat ttcattcatc
     3841 taaggtaatg tgatgtgatt ctgaaattct ttcaaaggca gtttttgcat aagctgacat
     3901 tacatcatca atgacatgtt tacaatgttt cactactaaa ataagggggt aaccaagaag
     3961 aaaaactgaa tgattatttg tatctaattc catggtatat caaccatttc ctatatagac
     4021 tgattatgac atggattaaa ataaaaaggc actatgtctt aattgtcggt tttcgagtta
     4081 ccgaatggca ttcatcaccc acctatagag gatgggcgac ttctgcctga aataagttac
     4141 actacttata acttctaata aaattagatt gagtcattat tatttaacat caacgatttt
     4201 ccagtttcca tttttttgta atactaattt gtactgaaag tagctagtgg tttttgatac
     4261 attatcaagg tattttatct ttgtactcac tagaaaattt tttccgtttt gtagataaac
     4321 tggattctct aactccgaaa actttaagtc aatattgatt ggtcgcatcg caccattttc
     4381 aacataatac tccaattctt tttcagaagc tgatggataa agtttaaaaa atgtttctaa
     4441 aaaatcggtg atttcttccg ttgttttggc atcaatttct gaatcgtttt ctacttgttt
     4501 tgcttcatat tttgccttgt ctggctttcc aatcattgtt ggcaaactag ttactaaatg
     4561 attcctgttt tcatcttcaa agattgtcac tcgatatgca gaagaaattg attgcgtctt
     4621 ttttccctct gtaatatgtt gctccactaa aaatgaaacg acaaattctt ccgagtgttt
     4681 ttcaacatct agaattttta cagattgaac ttcggaactc gtgggaatat ctgctcgaac
     4741 catatcttgg attaaagcga gaccgttttc tgtaaggtac tgttcgagat tactcattct
     4801 cttttcaatt gcttctttgt tatttttcca tgaaaaatat tctttcgcaa aatcctctgt
     4861 aaaactttca acgccagaag tattcactac cttctctttt ataaccactt tttcatgagt
     4921 tgtatgttga tcaatggcgg tgaaattttt ataaacgcca aaagcaagac tacaagacaa
     4981 taaaagccaa agggcaagca ccatttttcg atgcttaccc acggatacaa ctcgttcttt
     5041 tttctttttt gggctctttt ccttttgatt ccgttcaatt tttatcttca ttctttttcc
     5101 tcttttctct tatttgtatc ggccagctcc gataagatgc tgccgccaat agttttccgt
     5161 tagattcgtc caccccactg gatctcctgc atgaaacatg cgattctccc ctgcgtaaat
     5221 gcccacatgg gtcacataag taccggcatc ataggttgaa tgaaagaaaa ctaaatcacc
     5281 tggtttggca tcttttagat caatatgagt catggcatca tattgatcct gagccacccg
     5341 aggaagtttc aggtcaactt tggcataaca ccattgggtc aagccactac aatcaaaccc
     5401 cgtttctgga cttgagccac caaacacata ggccgttcct tcatacttaa atgcctcatc
     5461 aaaaatttct tggactattt tgtcatcaaa ctgagttgta gttaggtatt gcttcactaa
     5521 taaacagtaa aacatattgc cataattata ccgccaccca ccattttcct taatcgcgat
     5581 tgggttgggg taatcgactt tttgaccacc tgatttttcc tttgaaaaag attcagctaa
     5641 agcaaaacta tgcttcttcc cgtttttccc gacatgatct agatacccgc caccatagtt
     5701 ataggcctga ataatactat cttgatcaca ccctagttct ttggaacgct taaccaaaga
     5761 cgcaaaatag agacagcctt gtttgatgga tgcctcggta cttaatgaat taggtggtaa
     5821 tccggctgac tcagaacttt gcataacgtc ttccattgtg ccaccagatt ccacttgaat
     5881 aatcgccaaa agaattggaa tatactctgt tatatccgat tctttggcat acttttccac
     5941 cattgcacgg tgtttcaata cttcttctga gacactagtt ccgccaggag aagtgccaga
     6001 actaccattt ccctcactgt cttcaccaaa caacaaaccc acacacatca aaatacccat
     6061 aaaagagatg gtcagaaaag ttaatgaaat aacacccact ttcttcaagt tcattttttc
     6121 tcacctgacc ttgattttgg acgatggttc attctttttt gtactttttg attggtctgc
     6181 tttggcagag tggacctctg tttcacttgt tggaaagaat ttttcttact tgctgattgg
     6241 cttttccctt ttgaaatgat ttcttgacta gttagttttc gtttgatttc gggattcaga
     6301 tttgctctca caatccgctt tggtgcttct tttttcagca tcatatcttt ctgaggttga
     6361 gctgctttat ctattggaat attcgccctt ctttttccct gacgatttgt tgcttcactc
     6421 agtatcttgc gtttttcgtc cattttcgca tttcttcgtt gtcgttgttt ttctcgcaaa
     6481 tccactcgat ccgctttttc ttggaacagt ccgcgcttga attcttctgg tgcttttttc
     6541 gacttttcga ttcctttatg gagattgtat ttcaaattcg ttggcgtatt ccttacctga
     6601 tcctttactt gtttagtctt atctttcacc aaagcttggg tatccaaaac tttgccgatt
     6661 tttctcccag ccaaattcat tcgggattgt ttcttattat ttgaaactgg attctcctta
     6721 gttaagtcag atagtatttc gtgatcgttc ggtaatcgtg aattctttcg tagagaagaa
     6781 ccggatggct taaatttgga ttttgattgt tcctttgcca acttatttcc gactgctgca
     6841 ccaccaccaa aagccaaagc tcgtcccaca tttcgattga gttgccgcaa ttttcgattc
     6901 attaacattt gcggttttcg catgacgcgt cttcccattg actggctatc attactctgg
     6961 aggttgaaca tactcataat gtcgcccaat ttgaaataga taccggcaaa ggtcacaatt
     7021 tgcagaaaag caaccatgaa aaatggatag gttgccgaaa tattgaaaaa catcgtggag
     7081 atgctaaatg cagtcgtaat caccaaagta acacccgctc gcatcataat cgtattgaat
     7141 aaacgaataa tcgccttctt tcctaaactt tcataagtcg gcaacatgga caataagaaa
     7201 ctaattggca gaaacatcgc aaagataatg aagagaattt gcgagaacaa cataatccct
     7261 gttagaagaa aaacaaagaa ggaaatgcca atattgaaca ataccaaaaa gaccacggtc
     7321 cctaaacggt tcatggtctt ggtaatactg agattcgcat tgtcattgtc ttcaatctct
     7381 gccttgaccg cttcttcccg atctttgcct ttgttttcat ctggactcac agatagaatc
     7441 ttgttgaccc ggtcttcacc aatttcttct atgttggaat catcaaattg cagtagtaac
     7501 caaggttgtt ggacttgaat cgaaaacaaa ctatcccgaa tcaaatccac actatctttt
     7561 ccttggcttt ctgaattggg tacaacgatc tcggttccaa gggtaagggc cgcttcacta
     7621 acatctgagc taaagtcatt gatttttgtg atataggtag gcgcataagc aatgaacgaa
     7681 cccgatagta gaaaaatcat cacaaaatta atcacggctc gaactgcttt cgtggtttct
     7741 cgttttaata atccggtata cgccacatac acccccagag ctaaaatcat caataataga
     7801 aacccaaaat aaaatccgga ggtctggagt ccattttcag taataccagc caatgtttgg
     7861 atattctttc caatactttc agccgtatcc gaaatgaaat ctaatttgta agcctcttga
     7921 accacataac ccgtggcgtt cgacaaatac aaactcaccg tccaaatgaa attagtaatg
     7981 gcataaagac catacatcac gcttttccct aagccatcac cccaattcca aggcaaccaa
     8041 tcccaagagc tatccacaaa gaaatctagt tgatagttgt tcagtgaata tttggaatat
     8101 agatttcctg cttcaactgt atcatcaact agccctgtcg cctcggctac cgtaccaact
     8161 aaactcaata gaactatcag agtgagtatg ctaataaaaa cgattcccac aatccgaaaa
     8221 agtttctttt tcatcccctc actccattct ctcttgaaca ggtggacggg tatcaaaggc
     8281 atggaagagt tcttcaaaga ctggatgtac ctgaaccacg cctacccgac catacaaatc
     8341 ctgcatcaag cattggccat tttccaaatc tcgtaggcgt ttttgattgc tttcatcttc
     8401 tcgatccaag ccaaagaatt ccaaggtttt cttgatttca tgaatatcgg tactgcgaaa
     8461 cgcaaacttc aacccgatat tattcttcaa gcgttcatcc agtaaatcat cactattttg
     8521 cgtgacaaag taaaccccgg cattcatagc ccgcccagca cgaacgagct tattcgataa
     8581 tgttttccct tgcgctactt gaagaaaact ccacgcctca tctaagtcta caattttgaa
     8641 gatttctcga ttactatgga taaagtccaa ggcaaaagtg gaaatgacga ttagcatgga
     8701 aacactaagc aattccatgg tggtatattc ttcaaaagtg gtttctgcat ctggtaaaac
     8761 caaatccgct acttgaatga tatttaactg cctatctaaa ctaatcgact gggtaatcga
     8821 accatctgag aaaagcaaat gggcaaaatc ataatcggta aaggactcga tgtgatccgc
     8881 gatattttcc gcaactggtg agccttcttt tcgaagctca ttaattactt gtaataatcc
     8941 ccgttgattg ctttgggtaa ccgtccgaat ggccttacgc aacactggaa acttctcccc
     9001 atctcgacta gaaattcctg ttaaaaaggt cagaatatca attgctagac tttcagaatc
     9061 cttctttttc tccataatca cataaggatc aagtaatcct tgattttttg gcttactagt
     9121 taagttgaga atattgattt catccgcaat ttctggcaag gtttctttcc agccaccccg
     9181 ttcccctttt ggatcgacaa tcacagcttg tccaccaaaa aggacggcgt aataaactaa
     9241 gagattgtta ctaaaggact ttccgccacc gagtgaaccg aggaatgccg ctgctagcgc
     9301 attggtaact gaatccttga ccccttgtga ggccaatgcc ggtttcaggt aaacattgcg
     9361 ccccgtatct acgttataac caaagtaaat gccttccaac tcccctaaca tctgagtggc
     9421 gccaaaacca agtccggcga gaaaatcaga cgtaacatac tggatataat cattcatgta
     9481 tcgcttacta gaaggtaaga attcttcatg tagccccatc atatcgccaa aaggccggac
     9541 tagttttaca ttggtatcat cataaaaatc tagcacttca tcacaccggc gctttagttc
     9601 atctactgat tcggcactta ctcgaaccac atagctcagt ttgtacatgg attctttgga
     9661 ctgatccaag gtagtttcca attcatctac ggaatccaat gcatctaaga cattggaatt
     9721 tgtttcattg tcagattgat aggcatggtt gtccaagtct tttaattcct ttttcttatt
     9781 gcgaacggta gcaagtgcct ttttgttcgt cacaatctcc acattcatac tagtgtcaat
     9841 tgggaatgta aattgttgtt gctggtaata gaaaagttct gaggagggaa actccatttc
     9901 ccccacaatt gtattaattg ttaagtacgc tacatatgat tcatgattct catgttccat
     9961 gcataagtac cggggctttt cttcaatcaa acaacgactg ggacgtaata agtcgtaacg
    10021 tttcaccaaa gtttccgact tcagcttttt ctttggtagc tgaaattcat attcttcaaa
    10081 tggtgtaccc ttttcgccat agatatgttc aatcaaatac atcagatcac tcggtgtgac
    10141 tcgtctgact ttaaatcttc gagccaattt actttccatt agtttttcaa gcttggtata
    10201 tcggcgaatt tcttcattgg ataaggaaac aaaatcgccc atcaaatgat gattcactcc
    10261 atagacaaat tcttgaaagc cggagaagaa agatttcttg agattcttta gattgacctc
    10321 ttcatctgtc gcaatcagtt tgaagccaat aaaaaagcga taatcaatct gactatcgcc
    10381 aatcatggat actaatgcct ctgtctgtaa atcaattcgt tgtttcgcta cttctgttaa
    10441 tcgcccggta atttcctttt ttgatcgttc ttgcgtggca cgaatactac tttctgtggc
    10501 aatttgtaag gcatgaatct tgccttcacg attttgcgca atcaattgac gaaagttatc
    10561 gtggacttga tatttctgtt caggtgacaa aaaggagtaa ttgtaaggta ttaactcata
    10621 gtaggcaaaa cattctccct cttgattaaa gacaagatta ttttcaatat acttaatcgg
    10681 gtacatagtt cacactcctt accagagtaa tgggttcgtc ccatcgtttc ttctgttctt
    10741 tgactttctt tcctgcgaaa gtgaccttaa atcgtaagtg atagctaata aaagatcgta
    10801 aaaatcccaa tggtttctta ccgtcaaacg ttttttgcga catgaaccaa gtaactgcca
    10861 ccggtatacc taagtatttt aaaaaggccc cctctatcat agatagtgga ggcacatggg
    10921 caaatacgat gacaaacagc aaggataaaa caaaccaagc catctggtta aaagtcacag
    10981 gaaaaggcag ttgaaagtca ttaattgcat aaatgacctt ttcaactgcc caaatactgg
    11041 tgtagctttt tattttcttc atcaaatcat tcctttaaaa agaaggatag tttagaaatc
    11101 aactaccctt caataatttt gttaatatgg atactcaaaa acacctcgac tcgttaccaa
    11161 ataattgcct ccaatctcca agtctctacc acacgcatca aaatcaatat acgtcttcaa
    11221 gtgttctggt acttctccaa aatcaccagc ttctacaatc aagaaatatg ctacatcact
    11281 catatcatcg caatctggat aatagataat atcatccaca tgctctacca tctcctcaaa
    11341 agaattgaag aaagcatgtt gaatctcaga agcaacttca ccaactggcg ttccttcaag
    11401 ttcctccgct aagccacaca accgattaat ttcctcaatt ggcgtatact catcaatctc
    11461 aaagggaagt tcataatcat gaattgcata ttcctcgtat tcgtcattta acccaattcg
    11521 ttctttcact tcatcaaaat cgacaggtgg ggtaaaccac gcccccacca attcaccttc
    11581 gttatatttc ccgaggttcg caatataaac acgcatctgt tccatcgtgc ttccccctta
    11641 cgcaccgaca attcggttaa acaaactgag taagacatcc ttcaccccag aagcattaaa
    11701 gaccaatccg accgcaatca aagcaatcac caaaaagcca atcagtttgg aaaactcccg
    11761 cttaaatcct aaaaacaaac caatgaccac aattgccagt aagactaagg attgggcatt
    11821 ggttaaaaac cattgatata tggataggta agttcctgat attataaata tctcaaactt
    11881 ttccccccat tcacacggag cgtgcgactt tcaccgcact ccgcgttcca tcgtgtttat
    11941 attgaaaagt atttatgtag tagacctata tcaagtttaa ttcttgattg ccagctaata
    12001 ctctatattt atttagtctt atgagctgga ctttatttag cttcaataga gctatatatt
    12061 tttgtattgt ttcttctgtt ttagcgtgta ttagacggtg taccgcctct tttaccaaaa
    12121 taagattttt ataatcgtca tttccaccag ctccagttgg tacaatatga tgacaatgta
    12181 tttcattagc ttgttgaaat tcttccagag ttatagcaca ttttccatgt tgagcagaaa
    12241 atagcgaaat tcgattgtct gcgtattcta ttgtgcgatt ttccaaagat aatttcatta
    12301 actggtacat cagtaattta tcaacactta gattggtgtg gataaatttt cttccctcta
    12361 gagtgtagca attcgctaaa tacacctttg ccattggttt tctgtgttgg atatatccaa
    12421 cagggaaaat aggttcgccc tcgccagcaa cataccgaag cattttagat ttaccatatc
    12481 ttgttgcctc ctttgtcgta agttgtctgc ctgtttttct caatctgccc cttttgtttg
    12541 tacctaatct gttagtgaag atagccatta cagctctatt cagtttcata caatccagat
    12601 ttacattcgt tgcaatctga taataatttt gcatacctaa taccattgaa ttataaagtc
    12661 ttgtttcttc taattccgtt ttcccttttg agggagaagc aattttcttt gcctgtgata
    12721 ccagcttgtc tttttggttt ttgaattgtt tatcacttat atgtgattta acaactaact
    12781 tctttcttct cggtatcatt ttaatcttga aaccaagaaa gtcggagtat ctttttctaa
    12841 catttacaat tcttgttttt tcttgtgata cttccaattt taatcgttgt tcaatccatt
    12901 gagtgattgc tattttggct ttttcagcac tttccttgta gcggcagaaa actctaaaat
    12961 catctgcata acgaaccata tacatttcct ttaactttgt tttcttcatt tctttgtaag
    13021 cgttgctttt acatgggcta ccgcttttat tgatatgaac cacatagttt tttgtaacag
    13081 gattttcctg ccattggctt tctacccaat ggtctaactc attaagaaca atgtttgcta
    13141 gtagtgggga tattattccc ccttgcggag ttcctttctc tggatatgtt attgtaccgt
    13201 tagtcatttt gattggagct tttaatatcc tttttaaaat gaatatcaag tgcttgtccc
    13261 gtattcccat agcccatatc tgttttatca gcttggaatg gttcacgttg tcaaagaaac
    13321 ccttaatatc aaattcaatc acataatgta gctggctgat ttgtaggcgg ttataggttg
    13381 cttttattgc attttcaact gacctattag gtctaaaacc atagctgttt tcactaaatt
    13441 tagcttcgca gattggttct aatacttgtt ttatacattg ttgtataagc ctgtcccaca
    13501 tacaaggaat acctagaggt ctggttttac catttggttt tggtatttct tttcgcctca
    13561 ctggcttagg tcgataccca tgtggactgc ctgcaacaat gtatcttact tttttgacaa
    13621 ctgtttctgc tggcaatttg ccaatatctt taattattgt accgtctgtt cctgctgttt
    13681 tactgcctgc atttgctttg atatttcgat atgccagtaa tatattttct ctcgatagga
    13741 taatatccat aagatgagtg aaaatatctc cattcttact tttggcatat aaatcatcaa
    13801 aggtgttttg catttcgtaa tattcagcgt gtcttaaatc ttctacacac aatgtttcat
    13861 ttttattttt aggcataagg cttcactcct ttacttgaaa ttgagtgagc ttttttatct
    13921 tactcgtaat ccttattgaa acaacataat acttttctaa tatatacacg acttgagggc
    13981 attcctccat tctcattaca agaatttctt aggttcgccc tctgcttttc agcattagtt
    14041 caactgctta ttgttcttcg tcagtttatt ctcttgatga gaacccaata cctttcctcg
    14101 ttccgataat cttatctata catatatatc cttaggtcac tagctataag cctgctggct
    14161 tgataccgcc tgtaacgtta tatggcattt cataacctat attcttactt tgccacacca
    14221 gctacgattt ctcgtacctt tgcatttcta caaagttcaa atttagaccc gtacattcgc
    14281 tgtttcgtca gttccttata gaacattcta accatagata ttttatagac ccccgacctg
    14341 ttggatacgc tacccacctc tggatagttt tcgatagaag atatatgttc atcaactatt
    14401 acggtatctt tcagccgtct ttaccgagct tcaaacattc tgagtttcct catacaatgc
    14461 ttgtcggagg ctcagggcag gcgtttcaga gcgttacttc atcattccac ctgtcagatt
    14521 atcagttctc ctaataacca aactttttcg ttcttttatt agtgccttag cttttcacta
    14581 aggaacgagt cgcacataaa ttttgtccga aattcatctt gtcactttcc tttcttttcc
    14641 attgctcgat ccgctgctgc acttgcattc atcaagcaca tcacagtaac accaagcata
    14701 ctgccaccaa ataccaaaaa aatatctttt acaattccca tgacgattcc tcttttcata
    14761 taatcaaatt ttcaattgcc atactttgtt gctccaaaat ctttttatgt cggtctgata
    14821 agcgtgcatt ctgtaccata tcctggatat aggttgtccg attcaatcga tccaactcct
    14881 tagccatttt ccacgtaggg gcgacttgtc ggcctagcca attcaaggtt cgttcaatgg
    14941 tatacggttt tggctcggaa gttaattgaa tctctttccg attccgtcca ataaaacgcc
    15001 cccattgttc attcaagggc cagttggttc gccttttacc ttctacttta tccgcaaatc
    15061 gcaagtaacg attgataatg gaaaacgtgg tgctctcaat gctacgacct tttaacaaat
    15121 cttgaatcgc atgaaaagca cggtcatttt tcagtcgaat ctcaaaacga tttttcgtct
    15181 cagtttggtc taatggaatc cctaacttga tatattgttc atagtctttt tcatacaaac
    15241 agaaataaac ctcactcttg agactgccga tatataaggt attgcccatg ccgtcctttt
    15301 catcagcctt taaaagttcc ccggaacgat aacttttaaa ggtacgaaac aaggagatac
    15361 attcttcctt ctgacatttt ttcgttagct caggtatatc caatagcccc actcgatcat
    15421 tgattgccaa gtccaagcgt ttcatcacgc caccggcctt tagacaatcc tcgaaaaagt
    15481 cgtaccagct acgcttttga gcgagtaaaa aagtttcaaa ttgtcggcaa cctcgcccct
    15541 ttaattctaa aagcacacct ttatcttctt catgagacaa catcaccaca atatcaccca
    15601 tgacatactg ttcttgatag gagtaaaagg cataatcttc atggagcata tactcaaatt
    15661 tcaaatggag aatttcttga ataatttttc gtgcatccgt tgtgggaaag cgaatcctta
    15721 catagtcaaa cagtaacgtc aaaggcaaat cgggattaaa gctttccagt tggtcaaaaa
    15781 ttttcctcat ggtttttata gatgcggtta cgccaccatt ttctatctga tttaagtacg
    15841 gccgactaag tccacaagcc gtagccaatt tattttgtga aacgccgtat tcgttgcgtc
    15901 ggtcttttaa tagccgataa tctaaattcc gttgtgccaa gttctcacct ccaaattcaa
    15961 ggtgttaact ttttcaaatg agttaacacc tatacaaatt acttactatc tttgatacaa
    16021 caagcattct taatggttgc ttacttattt tttgtgtttt tgtacccccc tgttagatac
    16081 gggggtgaga catcatggtc ggctaacgcc gtccatgaac aggcacagca ccacggagga
    16141 tttctgcttc gcgaatcctc cgcggtcagc gcctaacagt cagttgtgtt ggtttaggtt
    16201 actcatctgt aataccattt acttccaact ctgctttcat ttctaacaag tattgacgaa
    16261 tatctggttc ttttgcttgg tccgcaatcc gctcaaattc tgcaattact tgatttattt
    16321 caattcgagc atccgtctcc tcattcatta aatatggatc gccagcgact ttgacaccat
    16381 tcttgcgaat atcaaatttt aaccgtcgca attgttcttc taattctggg tctgactgaa
    16441 tctttccaat cacccaataa atttctccaa tcaactcatc ataaaccatg tacatccaac
    16501 tcttttaatc ttctggcaat atcaaaaatg accggaactg ttacagagtt tccggcttgc
    16561 ttatacaact gactatctga attgacttga gaatctcgat caaaagccca atcgggaaag
    16621 ccctgaagtc gccaacactc tctaggtgtt agcttccgaa tttgatactg atccgtgacg
    16681 atcgcttgtt ggtctcctgt tagtaaggta tgaacactct gttttcccag acgacctcgg
    16741 cgacttgtgg aattaggaaa agccagattc acactgtctt ctggcttcac ctcttgatat
    16801 tctgtttcat tttttcgaat catgattttt gtaccacctt gtttcgttga caaggtgggg
    16861 gcggttccag taacttcata aactcgatta ccttgttcaa actttcccgg taatagtcct
    16921 gctacagaga ttccatccct tgctggcagt gcgattttga ttccttcatc acctgttaag
    16981 gttggcgcta cgccatctga accatagact tgtccaccca ttcctcgatt ggaaggattg
    17041 atatttacca aatttctgat gttttgatca gatgttgaag acggtccaat gataggaaat
    17101 acttgttcgg tacatgcgtc tcgagaatgt ccgataagga atatccgctc ccggctttgg
    17161 ggaatgtagg cctttgagtt aagcacctac cactccacgt cataccccaa tccatccagc
    17221 gttcggagga tcgtctcgaa cgtagcccct ccttcgtgat tgagcaatcc tcggacgttc
    17281 tcaaggaata aaagcttagg tcggagaaga gtggtgaacc gagcgatttc aaagaataaa
    17341 gtacctcgag tatcggaaaa tctttgccgc tttcccgcaa ttgaaaaagc ttggcacgga
    17401 aatccgccgc aaaggatgtc cactggtccg agggattgaa taaactcatc tgatacactt
    17461 gtgatgtcat gcatttccac ctccttactt gtatcatgaa ttgctttata actacgtcta
    17521 gcaaatttat caatttcaca aaaaccgata caatgatggc tcgcctgttc catacctaaa
    17581 cgaaagccac caataccggc gaataaatct aaaaatctca tacattacct cattcattgc
    17641 aaagaaaaaa gccaagaaca ttttgctctt ggctcataca ttattaattt gtcctactat
    17701 tttttattta atccaactaa tttccgttgt ttttcattgg cctgatcggc tacatattta
    17761 atcaccgttt tgaactcttt tgcagtagca ttttccgata aatccgcaat gctcttattg
    17821 tatttcttta caattttcgt tacttgttct ttctcagtca tgtaattgaa cctccaaatc
    17881 tttcctacta taccctagtg gtaaaaataa ttctagatta tccgtagcat aatgaacttc
    17941 ggaaatatta ttcgtataat cttttattat gatatgaagc aatacatctg gatgtttact
    18001 ctttactttt gcaatgttct ctcttgattt gaaaaaagcg ttaataaatg cctttttcgg
    18061 cacatgacgt ccctctgcaa tttcacgttc ttgtgtaaat ttccaagcga ttttagggtc
    18121 ttgataaaca tagaaaatcg tcactcgaaa atgttttttc aacgcccgcg agatattttt
    18181 ttcagcactt aatattgcaa aagttgcatc cagaataaaa gaatagccat gatctaagac
    18241 atatctaaat gcctgctcta ccaaccaaga agatgctttt tgaaagagac tagagttttt
    18301 tccattataa tcaggaaact gggtgcgaaa gtaatccgca tcaataatca catggttatc
    18361 ataattctca gctaatccaa tggcaacctc tgattttcca gccccaggac tccctgccat
    18421 aaaaactgcg tctcgtccaa cttcggggga tttccctttg ataatataag aaataaactt
    18481 atccttattt gcttttgcat actctaaata agaatcatta ctttccatat taattatccc
    18541 ctttcttcct actatataaa agtattatac gttacttgaa gtataaagtc agtattgaag
    18601 atcactcaat cattcatctt ttctaattta ctaattgaat ctaagaaatc ataccctttt
    18661 ggtacaagtg gcgtataaaa ctcactaatc acactgccac ctgtatccac ataacctcga
    18721 cctttgatgt gcttcataaa gaagtttttt tcgacctccc caaacatcat ggaatatccg
    18781 agttcactca tacgacccaa agccacgcga aagttgaatt gatcgcggat tccgtctcct
    18841 aaatatttcg catccggcct ttggcaagcc aaaatcagaa agaaaccaga ttgacgaccc
    18901 aacatcacga tttgtttgag cttattcaaa atcaccgcac tttctttcgt cgttaacatc
    18961 tccatataag ccacgtattc atcaaagatt aaaaagttgg gtggaagtcc aagataggca
    19021 taattctctc ctgttttgta gttagacatt tctttcattg ccttgctacg agccatcata
    19081 cgttcataga aatcttccac acaagcagaa atttcttcct tttgtgaata aacatgaggc
    19141 atcaccgtac ctaaatcagc taaatctgca tttttgggat cgaggataaa caattctgca
    19201 tctgacttca atagtgcttc gataatggtg aggaggaaat aggtcttccc tccacccgtg
    19261 ccaccagcga ttaacatatg gggtaaagaa tcataagccc atacttgatt tttcatcaat
    19321 cgcagagtcc cattttctgc cactacttcg tctatcccaa ttcgattggc gatcatatca
    19381 tacagtaaag tatactccac ataggagtct ttgagttcct tttctactaa ctcacaatac
    19441 agtccgcttt ctaacttctt ttccaatttt aaaagctgat cttggtattt cccaagtgaa
    19501 atttgaacgc gaatagacag taatccatct ttcattcgat aacagatttt ggggaagtaa
    19561 ctgatagtct ctctggttcg gctactattc aaatctttga aaaagccttc actttgagtt
    19621 tgtttcactt cataccaatg gttctctaga atcatccgag ctagcttttg tcgatgccac
    19681 aagcttcgcc aatgatcaag aaaaaagtga atataaagca atatcatagc tcctgaaaaa
    19741 gcgagacttc caagcagaca tactaaatct tttatagacc aaacgatttc aatagtagaa
    19801 agtgatagct tagaaatatc aaattgtcta aactcttgaa aataaaagac gctcatccaa
    19861 ccgatgaaca gagcgagtag ccagccaaga acaaagtgat aaaccaagtg ctgatctcct
    19921 gcgcgtatgc gatgtccttt gtacattttg atggtttcca cctccaatcg aaaaagccca
    19981 ataggatttc attaggcaat ttcaaaaagt tatgctagtc atttactgct acgaggaggg
    20041 gagttgtttc gtttcaacca attgatttaa aaagccttca atagcatccg gcatctccac
    20101 accaatttct tccaaaatgg attctgcctg ccgacataag ttttcattta cttcgacaac
    20161 tataaagaaa gactcttctt ctcctaacca gtctagtaca tcagagtctt cttcactgtc
    20221 gctattttct ccaagtaaac cttgatgtat aatcagaaac attttttcat cagaggttaa
    20281 tgctttaacg agttgttcct cagtcagctc gccttttcca tattgatcga gacaatctaa
    20341 aatatgttca acattaattt ccataatctt cacctatttc tttctttcat tttggttcgt
    20401 aggatttcct tgattcggct gccgcttcaa gactaaatca tccgccttaa tgaaccaatt
    20461 aacggtagtt ccttctcgaa aaacataatt ggcaacggta tccacgattg gattgaccaa
    20521 ctctacctct gcatcataag caaactcgcg taagggtaca ttagctggga tactaacttg
    20581 aatcattcgc ccttgtgcag aggattttaa attataggtt cgttctttta cctcattacc
    20641 aattgttcca tcctcatttt gcttccgtac ttctcggcgt aatgcggaaa aacgcaactt
    20701 cccaaatgtt aaacctgaat ctacgacaat accttctgtt aatctcatct tcttttcctc
    20761 ctatgctttc actaaatcat caacatacaa cacgtaatca gtaaacccac gattctcaat
    20821 ctcatagcct ttggcaacca aatggggatt cacggctttt agcggttggt cttgatcaaa
    20881 atccttttca ccggcttccg cagctactac cacctcaata tcatcggccc gttgcttact
    20941 ggaatacaaa tgataactgc gaccaatcac cgtagtattc cgtccatatc cttctgttat
    21001 aatttctcct tcaccggcat aacttaactt tccaaaggtt tcagccatat ctggcacgac
    21061 aaatttcaat tccatcttct tttcctcttt tcattgttag ttttggatac aaaaatagcg
    21121 cgactacttt tttaggaggc ggtcgcgcac gttggttctt aggatttact tgtatcatct
    21181 catcacctta gttccggcga ttccagaaat aatagcccgc cgtcgcaaaa atcaaagtaa
    21241 aaccaatgaa cagtaaaaca ttggaatttt tctccccggt ttgagggaag gtcttcactg
    21301 tgggactaga ttcttgagag ccacttggaa cagttggtgt ttccggttgt ttcggcgtac
    21361 ttagtatggt tggaacttct tttggtgtta aggtttgaga tttttctttc aaatcttcat
    21421 tgtgttttcc attgatattc ccatctttat cgtaattgat ttccgcaaaa gtaaacttgg
    21481 ttccttctgg atattttccg gtatctactt ttttcgcaag cacagttttg gtgaattctt
    21541 tatcatttac ttcataatca atcttgccag atttccaaat ttctttgttt gtaccatccg
    21601 gtaataaagc atacagaatt gtttcgaaag cttcttttga gccatccagt acatcatgag
    21661 taatcgacac atcatcaaac atatccacca catcaccatg agtaaaggtc tgagaaccgt
    21721 tttctaggtg ggcctttgtt tggatagaaa catggtgttc cactgtacaa ctgacagttt
    21781 gggcttggtt attcaagcta acatccttcg ctactggctt atcgcctgct tcaaaagctt
    21841 ccttattttc atacacatag ttgaataaga caatagtttt atttaaaact tgttcggcgg
    21901 ttaatttatg ctgaattttc caagttcctg ttttctcttt gttcgccaac gttgcgcttg
    21961 tttctgcaat caccactggt ttggcttttt catctttttc ttgggcagct tttgtcgcat
    22021 ccacatcaat ggcttgcgct accacatacc agtcttcttt gatttcatgt agattgtaac
    22081 tcaatttatc aactagctcg gtagaatcca gagaagttaa ttctttgttt ccgtctttcc
    22141 aagtcgcaag agaagttaaa ctatcttctt cctcaggcaa atcatagagc attaaacggt
    22201 tcaaactaac agaaaatgct ttgttcgtca gcttctcgta aggaacggtc accgttttaa
    22261 ttggttgttt ttggtcttgt tcggtaatgg taaagacata ctcactcttc acaaagtctt
    22321 ctttgttttc cttaaaagta gaacggattt ctaatggcgt aatcttttga aaaccttctg
    22381 gggcttctac ttcttcaagt aaataatccc cataaggaag attttcaaac ttgccataac
    22441 catcaaaccc taattgctcg ttataagctg tggtcgcttc atcttcggca cctgtgattt
    22501 cattggtccc ttccaatggc gacactttaa aggtcagatc attgaagcca gtttcggtag
    22561 tgcccgcagc tgatccagca aatttaaaga aatcaaagcc aaagcgaatc acttgttctt
    22621 ttgccgtaac atttcgggta atcacggcat ttttttcgct atcatccacc tttttgatgg
    22681 atacaggata ctttgtttca tcaaggctat atccttcggg cgcttttgtt tcttgccaat
    22741 aatactcgtt gatagctaaa tgtttaacgg caacttggtt cttttcgtct aaagtcaaag
    22801 tgaccgtttc atcagaacct ttcgttccct tcgctaactc tggtttaaaa gcctcactcc
    22861 atttaacagc ttttccatcc ttagcagtaa acaaggtgta ttcagcaccc tcaaagactg
    22921 ccttaccttg tgctttatcc ccagtatctt tgtcttcttt cgtcaaagtg gtttcgccag
    22981 tgacttcttg attttgccct tttacgttac tggtaacagg ggctacggtt tgattggcat
    23041 acttcaattc gacttttaca ggtttgaacg tattcacgaa cccatgacta gctttagttt
    23101 cggtcacata ataggttccc aattccaacg cattagcaat ctcttttggt gtttccgcat
    23161 gaccgttttc atccgtggtc atttcttgaa cgatttcacc ggttggactg tctttgcgaa
    23221 tggcaaacgt attaccagct aaggaataat tgtcattcca aagatcactc cctgtttcaa
    23281 ctccgctttt atccagaatg atttgccctt tttcccgtgc gttttttgaa gtcacgtaaa
    23341 tggtttcgcc tgctttaatg gtggtagtca tgggggtggt atcaatcgta taaggcgccg
    23401 gtactgattt ttcggtaatg gttacttttg taccatgagg aatctcatcc aatgtagcaa
    23461 tgccttcttt gtcagtagtc acgtcttttg caggtaaagt ttttccaaag tctaaatgga
    23521 aaaccgttcc tggtacaaca tcacccgatt ctttatcgac ttttttgatc tttaaagaac
    23581 cctccgtttc cacatcaatt ttgattgtgt aggtattagg tttatcaatc gccccggcca
    23641 ttaaagtctg ttggccagcc tttttatagg cgacaggtgt tccggtacca gcagatttct
    23701 taagcgtcag cacaccactt tcttttgaat tggcatttgg ggtgattatc aactgattac
    23761 cattcacacg ataatcaatg tttgcggcat tttccacaac ctcatcgaac tctgacaaat
    23821 tcagattatt cgtatccgtc acagtagtcg attgccctaa tactgtttta gccgtactat
    23881 tatggaaggt tggtttcttc tgataatcgt caatggcttg atttattttt gcctcaatgg
    23941 ctgcgatatt tacggcacta ccatctgatc gttttattga gtgaagggta tacccattaa
    24001 cttcttgcca gatcatcttc tgtgccacaa tatgagtatc tgcatctgta cctacttttt
    24061 tccataaaac ggaaactaat ttggctttat cggacatatc tggtaacggg tttttctcat
    24121 agccaggagt gaactcattg taaatcggga ctcctggttc aatacagaaa atatcttgtt
    24181 taaagccgtt gtacactgca taatgcggaa cagtgacttc tgacattgga cgaccatcac
    24241 taaaagttcc ttcaacagca tattgatgtg ctaaatctag ttctacggtt accgtttgtg
    24301 gatgggtaat ttcatcagca aaagtaatcg taggagcaat tgctccaaca attgcttgaa
    24361 cagccaaact caaaatagtt aatacacata atttcttttt ttgtttttta tacatgaaat
    24421 gttctccttt caaattaagc acaaaaaaat aagatgctcg cttgaacatc tttgtttata
    24481 aagtcttttt aaaattttta ttgactgaat agaatggatt tgagaatgaa tgagcctaaa
    24541 aatttaatct aaaatacgaa atatactgcc tttaaattag aacacatttt agaacacaaa
    24601 catttcaaat ttaatcaaat ataataaaat atatttacgg gaaaaccttt ataaatcaag
    24661 gtttttcaaa cttattcaat gatacgaaga aaaaaccagg tcttaagaaa gcccgtaaag
    24721 cttcacagtt ctcaaaacgt taattgcgat tatcgttcga gttcacgagt tacgagaatc
    24781 gtactgggcg aagcaatgct gagcgcagtt accttaatcc taacttttct ggcactttcc
    24841 acaatttgga aagtgccttt tttgtgtttt tagctatgtt aataggtagg attcttggtc
    24901 tataatatca ttcgatctat aaacaaacgg gaaaaagata aagtatcatc aaacaatatt
    24961 atttatccgc ttctgaggtt gtgacagaag tggtcagctt caagaaataa gaaggaattt
    25021 acaaaaattg cttctcatgt ttgataaata ttgtgacttg tgaccgagga gttggcagga
    25081 gaacaccgtt tattcggaag aagggattgt gaaaagtctt ttgtcataac ctcttatttg
    25141 gtagctaatt gtaaatccca aacggcatgt attatttttc ataagtccgt ttttaaggat
    25201 taatgttttc ttttgctttt tatatggtct ggcttcagcg agaagttcca acgcatatac
    25261 aaggacgact tatgtcaacg ataacaacga ttaccatggt ggccagtcca cttggattag
    25321 cactgtttgg tgcgctcttt gattggaaga caacgcaacc acgtctgcga gacagtctaa
    25381 tttttctggg agggagtctt gctatagtta tccttataac cggtctggta aaattactca
    25441 agcttgatct gaaagaagca aaaattttca gtgaagagga atacagagaa acgactttgg
    25501 tcgaaaagaa agaatcagga aactgataca agcgtataac aaactgcctg ataataggaa
    25561 agacacgtat tgtccgattg ttttttttga cattcaattg gaaaatttac actttcttta
    25621 tacatttctg gctgctttct ttatacccgt caactatact tcactcatca ataagaaaga
    25681 aggataagta tggattttgg aaatgaaacg gtcttgaaag caactggtat aaccaaaaaa
    25741 tacggtgctg ccaaagcatt agataaagta tcaatcgaaa tcaaacgtgg aatgatctat
    25801 ggattgattg gggaaaatgg agcgggtaag tctaccttta tgagaactat tatgggtttg
    25861 atctcgattg atgaaggttc gatcgaatta tttggaacga cggatttgca ggcagcaaga
    25921 cgacgtatgg gtcaatcgat tgaaacccct gctctttatc cagaattaac tgcaagagat
    25981 aacctacgta tccaagcagc gaacggcggt gtgtcggata gagaaatcga agacctgctg
    26041 aaaatgatgc gattggaaaa tacagggaaa aagaaagcaa agaatttctc tttaggtatg
    26101 cgccagcgtt tagctattgc aaatgcactg atcacgaatc cagaattttt gattttggat
    26161 gaaccgacga atgggatgga tccagcaggg atggcagaaa tgcgagaaat catccaacgt
    26221 ttggtaaaag aacggggcat caccgtcttg ctttctagcc acttgctaga tgaattgtca
    26281 cagatcgcca ctcactatgg tattttgcat gaaggacact tgatcaaaga attatcgaaa
    26341 gaagaattgg cacaagaatc tcgtcaattt ataaaaattg acacttcggc gacggaacaa
    26401 gcagtaacgg ttctcgactc tcttggttat agggattatt ttgtacaatc tagccgtgtt
    26461 atccagcttt ttgaaggaat tgaccaagta gcagccatca atcaagcatt ggtagaagca
    26521 aaagttcctg tggatggtat tcacctagtt gggcaaaaac tggaagatta tttcttacaa
    26581 ttaactggag gcaatccaca tgtttaatct attaactgct gaaaaaataa aagtatggaa
    26641 gagccgtaaa atgtgggtct cactaggact gatggtcgta cttccgcttt tttattcttg
    26701 gaacgaatgg tatatgcata caaaatacgg caatgaatta aaccaagcaa cggatacagt
    26761 catcaacggt gcaacaggga ttttaatggt agaaaaaatg gctgcatgga ttcttctggc
    26821 tttcgcagca tttgcgtgct tttatattgg ggaagaattt caaaacggta cgattcgcaa
    26881 tacgttatct ttaggacgga atcgaatgac ttattatgtg tcgaaacttc tcgttacgct
    26941 tttgatcaca acacttggcg tggttttgat cacgggacta gccatgcttg cttacacact
    27001 tgctttcgga tttggtgaag ttgagggaat caaaaactat ggaaattatg ttctaaaagt
    27061 atttcctgta ttgcttctgc tgacattagg gacattatct gttccagtgg ctttgacatt
    27121 catcactaga agtaccagtg tcagtttgct tctttctttt ctttatatca tgggaacggc
    27181 cttcattccc ggtgttttcg caaaaatcaa aggcttggaa tttctaacgg agtggtttac
    27241 ggaaacgtgg ctcatgtata cggactttgc gcaacaagca acttattcgc aagcaccgaa
    27301 aatgattctg gtcagtctag taacaatcgt ggtatcatca gctctaggaa tgtttatttt
    27361 ccaaaaatct gacattaagt aggtggtcct gctggaaact attttgatta tcgaagacga
    27421 tgaagctgtt cattccttac tggaggaggt tcttgaacag agatacaaac ttcttgatgc
    27481 ttattcaggt acggaaggaa aattattgtt aagcacatat cctgtcgatt tgatccttct
    27541 agatctcatg cttccagggc tttcaggtga agcattgtta gcagaaatcc gacagacatc
    27601 gaatgttccg atcatcgttc tttcagcaaa aagcgatcaa agagataaag tttccttgtt
    27661 ggcagcagga gcggatgact atgtgacgaa accatttgat atcgaagaac ttttgttacg
    27721 aatcggcatc caacttcgtc atcagacttc tgtccaagta aaagatctgt cgcaacgcat
    27781 ctattacaag gaaatcttac tggacaaaga gtcgagggat gtaaatgtga atggtgtggc
    27841 tgtccatttg acaggaagag aattcgatct gctgaagctt tttttagaac atccgaaaaa
    27901 agtattttcg cgagcaaatc tttatgaaac agtttggcaa gaaccatatt ttgatagtga
    27961 gaaaacagtg aatatgcata tttccaatct tcgagggaag ctagcaaagg cacatgctgt
    28021 gtatattcat acggtgtggg gagtggggtt tagatttgat taaatggctg ttgcttctcg
    28081 ttgttatctt tatgggaagt tttattttct tcctgcaaag ggaaatcaag agattaacta
    28141 agaaaatcgc cactctttct gaacgacaag aatatggtgg tcgtctttca gtcgaatttc
    28201 gagaaaagaa tctaatggac ctagtagatg cactgaatac atggttggat gaaaatgaac
    28261 aaaaaggaca agcatttcaa gaaatggaag aaaacatcag actatcgatc gtcggccttt
    28321 ctcatgatct cagaactcct ttgactgcta ttaacggtta tgtacaatta ctggaacagg
    28381 tagaggatac ggagaaacgg gagaagtatt taacgatcat caaacaatct gttgcaagat
    28441 tactcagtat gacggatcag ttttatgatc ttgctcgaat agagacgaag caaaaagaaa
    28501 tggatctcca tacgctttct ttttctaaag aggtggaaga agtatttttc tctttttatg
    28561 aacaattttc tgagaaaaat atcgaggtag cgttcgctga acactcgata aatacaactg
    28621 tattagcaga tccgctcatg ttgctgcgag tgctgcaaaa tatcatacag aatttattgc
    28681 gttatgcaga tggacaagca aaggtgacat tttcaaaaga ggatccattc attcgattaa
    28741 cagtggccaa tcctatcaaa ccaggaacgc aaacctctgt tgaaaaagtc ttccagcgtt
    28801 tttataccga aaataccagc cgcacaaata cggaatctag cggaatcggg ctttacctgt
    28861 caaaaaaatt ggtggaagga atgggaggag agatgaccgc tagattagac gggggcatct
    28921 tctccatctc cgtcaagttg cggagggttt aattatacaa gattagtgag aactagtaga
    28981 aaaatgtatt atcttatata tatttaatag agaaaaaagc ctgtgatata aatattgcag
    29041 gcttttttat tgatttgaaa taatcaagaa tattagaaac ttctacatta atcaagaact
    29101 ttcttctgta tgaaaataga g
//
LOCUS       DASJSZ010000030        28582 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.030, whole genome shotgun sequence.
ACCESSION   DASJSZ010000030 DASJSZ010000000
VERSION     DASJSZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.030"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(18..710)
                     /locus_tag="U4B03_001236"
     CDS             complement(18..710)
                     /locus_tag="U4B03_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2068099.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            705..971
                     /locus_tag="U4B03_001237"
     CDS             705..971
                     /locus_tag="U4B03_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2068100.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1147..1716)
                     /locus_tag="U4B03_001238"
     CDS             complement(1147..1716)
                     /locus_tag="U4B03_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2068101.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="U4B03_001239"
     CDS             complement(1790..3079)
                     /locus_tag="U4B03_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068102.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3433..4281
                     /locus_tag="U4B03_001240"
     CDS             3433..4281
                     /locus_tag="U4B03_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEN2068103.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4426..5367
                     /locus_tag="U4B03_001241"
     CDS             4426..5367
                     /locus_tag="U4B03_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN2068104.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="U4B03_001242"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="U4B03_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="U4B03_001243"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="U4B03_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEN2068105.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="U4B03_001244"
     CDS             complement(9831..12017)
                     /locus_tag="U4B03_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN2068106.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="U4B03_001245"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="U4B03_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEN2068107.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="U4B03_001246"
     CDS             complement(12239..12679)
                     /locus_tag="U4B03_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEN2068108.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13226)
                     /locus_tag="U4B03_001247"
     CDS             complement(12753..13226)
                     /locus_tag="U4B03_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HEN2068109.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFIVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(13536..15002)
                     /locus_tag="U4B03_001248"
     CDS             complement(13536..15002)
                     /locus_tag="U4B03_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2068110.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15313..17394)
                     /gene="cadA"
                     /locus_tag="U4B03_001249"
     CDS             complement(15313..17394)
                     /gene="cadA"
                     /locus_tag="U4B03_001249"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN2068111.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17918..18277)
                     /locus_tag="U4B03_001250"
     CDS             complement(17918..18277)
                     /locus_tag="U4B03_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEN2068112.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18307..18648)
                     /locus_tag="U4B03_001251"
     CDS             complement(18307..18648)
                     /locus_tag="U4B03_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068113.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(18645..19319)
                     /locus_tag="U4B03_001252"
     CDS             complement(18645..19319)
                     /locus_tag="U4B03_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2068114.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20253..21551)
                     /gene="asnS"
                     /locus_tag="U4B03_001253"
     CDS             complement(20253..21551)
                     /gene="asnS"
                     /locus_tag="U4B03_001253"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEN2068115.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(21591..22781)
                     /locus_tag="U4B03_001254"
     CDS             complement(21591..22781)
                     /locus_tag="U4B03_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2068116.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(22802..23329)
                     /locus_tag="U4B03_001255"
     CDS             complement(22802..23329)
                     /locus_tag="U4B03_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEN2068117.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23376..26165)
                     /locus_tag="U4B03_001256"
     CDS             complement(23376..26165)
                     /locus_tag="U4B03_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2068118.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26312..26512)
                     /locus_tag="U4B03_001257"
     CDS             complement(26312..26512)
                     /locus_tag="U4B03_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2068119.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27103..28209)
                     /locus_tag="U4B03_001258"
     CDS             complement(27103..28209)
                     /locus_tag="U4B03_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068120.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
BASE COUNT         8030 a         6066 c         4993 g         9493 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtagctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttcctttttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttt ggttcattgt ttccttcaaa atgccagata
    12901 aaagataaaa attctaagta tttcctgttt agtttctccg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaac
    13081 gtagaagtat aatttccttc aacgatccat gacggattag atactataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaatg cggaaagccg gtgatttctt tgatcttcct
    13201 tgcgagggac gattttcccg acccactttg tccaatcacg atgatcttca ttcttctccc
    13261 tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa aaaacaaggg
    13321 actgtgacaa aagtcgtgtc aaagtccctt gtttcgaata aatggtgttc aatcagctga
    13381 ccttcagcat tagctcaaaa aacggcaaaa tatgaggagc tatttttgtt tggctgttct
    13441 tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc cagaagtagc
    13501 ttctgtcaca acctcttgta acaagtattt ctattttaag gctgaaccac tgtttctggt
    13561 tcagtaattt ctttgttttt gtctcgacga gcaattgctg gcaagatcat acctaataag
    13621 atcaagacaa ttggtgtgat gatattcgtg aataattgga accaccatgc tttaggatca
    13681 gcagtataat ctacttttgg caccattcct aggatacatg caaacgcagt gaaggcaaag
    13741 caccaagcac caaagataaa accgattttt ggatttttaa caaatttata ttcagaattg
    13801 aatttcttat aagctttatt caacatcata tatgccaaga acacccatag gtaacgcatt
    13861 ggcatgacaa ctgagttcaa gttcgtcaac cattttacta attcattcat atctttgata
    13921 ccaaagatcg gcaacaaaat aatgattcct actagtatac ctgtcagaag ataaccattc
    13981 accaatgtac ctttacgatt acgtttacgt agccagtttg gtacaaattc tggatccgca
    14041 tctgctaaca agatttttag tggtgcatca attgaaaagg ctaatgcgga gatttgtcca
    14101 atcatgtttg tcaatgcgta aatgatcatc aatgagttac cgataccata gtaattacct
    14161 aaaatttgaa aagcactgta tgccccattt gccatcaagt ctttagggat attatcgcta
    14221 gcaaacagca tacccattgc gactgagcct aatacagcac aaatccctac cattgctgct
    14281 aagaagatca tccctttagg aaattccttt gcaggatttc ttgtagagtt gacataaggg
    14341 gatatttttt ccgcccctcc aactgcaaat actagcatcg aaacggttgt gaaataactg
    14401 aaatcaaatg ttggaatata tgttttcact tgattcatat gtggtgtggc aaattccatc
    14461 gttggattga tgaacggtgc cgttacagct aaaagaataa ataagatcga catgacgaac
    14521 atcgctgttc cggccagtcc gccaatcact ttcaaagtag aaagtccttt tgttgataaa
    14581 tataaaaagg caagaaatat aataagtgaa ataactgcta ccatttgtac tgacatcgta
    14641 tttacgatat ttccatttcc ttggactgcc caaccaccag caatcagaat tgcttgaggc
    14701 ttttgtgcca aataaggaat atggacgacc cagtaagtcc aagcggcata gtatgccagt
    14761 cgtttattag atgtcgtatt ttcgacccat gaactgacgc ctcctttact atccttgaag
    14821 gctgatccta attgtccaac gatcaatgcg tatggaataa aataaatggc caaaatcaat
    14881 agccaagagg tgacaactga tactccttgc tgagcatagt tattaactac attccccatg
    14941 ccccaaacca tgttaaatgc aattaacgca acagtaaacc agcgtaattg tttgttgttc
    15001 attcgttctc tcctttatga taaaaatttg ttatccagat gtaatcatat tcaattttta
    15061 acaaacttac aagacagtgt tattttatgt gtgtattatt ttatttaata cacatcgttt
    15121 tttcatattt tttccatcaa aaaaataagt tattttcttt tatttcattg accttattga
    15181 atttcaagct attttgaatt ttctgcaaaa ttaaaaacat tagttatttt ataatcctat
    15241 ctattttctt tacatttttc attaagaaaa ggatataaaa aaaagaggac gttcattcgt
    15301 cctcttcatt gatcaacgtt tattttttac atgcaagctt cgcatggcat tcaaaacagc
    15361 taatactgtc accccaacat ctgcaaatac tgcatctccc attgatgcaa aaccaaaagc
    15421 acctaaggaa agtaccaaaa tttttatccc aatagcaaag acaatgtttt gtttgacgat
    15481 tttcaatgtt ttctttgcta aatggattgc ttctgcaatc ttcgatggtt gatcattcat
    15541 gatgactaca tctgctgctt caattgcagc atcgcttccc aatccaccca ttgcgattcc
    15601 tacatctgct cttgctagca ccggtgcatc gttgattcca tctccgacaa atgcggtctt
    15661 tttattgtta ctttgtaaga tttcctctaa ccgctgcact ttatcttctg gaagcaattc
    15721 gctatatatc ttgtccacgc ctacttgctt cccgatatga tcagcaattt ttttcgagtc
    15781 gcccgtcaac atgactgtgt ttttcacacc agcctgtttt aattgaacca atgcgtctac
    15841 ggcatcttct ttcaaggtat ctgcaataac aagataacca ctgtaacttt gatcgattgc
    15901 tagataaagc aatgtaccga tttcttgact tgaaggaaaa gagatttcaa atctttccat
    15961 caatttgtga ttacctacta atacttgttt gccatcaact tcagctaaga tcccttcacc
    16021 tgcaaattcc tggatattag ctgttggtaa aagcggacct gcgtagcctt ctaaaacaga
    16081 ttgagcaatc ggatgtgtag aaaactgttc tgcactcgca atatattgca tgaatatctt
    16141 tggatcgatt tttgtatcga tcgtctggat cgaaaagtcg cctttcgtta atgtgccggt
    16201 tttatcgaat accactgtct cggtttgcgc aagcaactcc aagtaatttg aaccttttac
    16261 taatacaccg attttacttg ctcctccgat accgccaaag aaactaagtg gcacggaaat
    16321 cactaatgca catggacatg agatcactaa aaatgtcaat gctcgataga tccattcaga
    16381 aaacgccgct ccagaaacaa caagtggagg taaaacagct aataaaacgg ccaatcctac
    16441 aacgactggt gtgtaatagc gcgcgaattt agtaatgaaa ttttcagctg gtgctttttt
    16501 actgctggca ttttctacta attctaataa tttgctgata gtagaatttt cataagattt
    16561 ttctacttga actaaaaatg gtttattttt attgataaat ccgcttaata ctgtttcacc
    16621 aacatataca gatttaggga ctgattctcc tgtaagtgct gacgtatcga ctaatgattc
    16681 tccttcgatg atcgttccat ctaacggtac acgttcacca ggcttcacaa gtacaaactg
    16741 accgatcagc acgtcttcag ggttcacggt catcaatcct tccgccgtct gcacgtgagc
    16801 tgtttctgga cggatcgcca tcaattcgcg aatcgatttt cttgaatgac tgactgccaa
    16861 tccttggaaa tactcgccga tttgataaaa tagcatcact gctacggctt ctggatattc
    16921 accgatcaac atagcgccaa tcgtagcaac agacataaga aaattctcat caaagatttt
    16981 tcctttgacg atattcatca ctgccttctt cactacatca tatcctatca gcagataaat
    17041 caataaatac cctatcaagc gaattagttc attaaaatca aataatagca ataaagcgat
    17101 acctgcaagg gaaaataaaa tccggaacag cgtatttctc tctttttcat ttccatgttc
    17161 agatactttt tccttatcta tttctgttac tttgacatct ggttctaatt ccttcactac
    17221 tttttttgtt tcttgttcaa tcgtagccaa atgatcttcc tccgcgtcaa tcgttagttt
    17281 tccagaagtg aagtttaccg ttgcttcctt gactccattg attttctgaa ctcctttttc
    17341 gattttcatt gcacagttcg cacaatctaa accttctaat ctgtaacttt tcattttaat
    17401 gacctcctcg cctcaacata tgaatatctc ttcataacta attataaatg aatggttatt
    17461 catatgtcaa ggtatccgaa agaaaaaatg aaaaacatct gcatcggaaa atttcttcca
    17521 tacagatgtt ttggcttatt ctttcttttt ctcttcgatc attgccactg ctcttatcca
    17581 acccgcactt gccttcttga tcttgacagg atagcaacta agtttgaagc ctgttgctgg
    17641 caatttgtct aaattcatta atttttcgat aggacaatat cttttttcaa tccctgcaaa
    17701 atgtccttcc cagatatacg aagctccact acgctttcaa aaatatagaa agagttcttt
    17761 aatgaaaatt atttcaactt tccataaaca gtaaccccat ataaataaaa gacgtaaaag
    17821 tcacatggaa gaacttatac aggaaaacgc atagagtcag gtgaaataca tgtatcccac
    17881 ctgactctat gctttatcta acctttatta ttctctttta tacaatttct gcaccgaatg
    17941 gcatcaacgc caatcgagca agtttgaaag attgcttggc aaaaggtatc cctatgatcg
    18001 tgatacatag aaggatgccg ctgacgacat gtgcgagagc aattggtagg cctgaaacga
    18061 tcagccagat gatattaacg atcaatgaca tgccagactg gtgataaatt acttctttgc
    18121 caaaaggcgc aaaagaaagg aaggcgatct taaaacattg cataccgatt gggataccta
    18181 tgatcgtgat acaccaaagt atccccgctg ctaaccagct aagaccaccg attagtccgc
    18241 cgaatataaa ccagatgaca tttcctaaac aactcattgt atttctcctt ctattactgt
    18301 ttttgattaa aactgggcaa aaattgattt tattcttctt tttttagctt ccagcatcaa
    18361 atattctgca aatttttttt ggatttcttc cgtattttct tttgttacta atgtgtgttg
    18421 gattagtttt cctgatggtc gctgacccac ccgttcttct gactgttgca cgtccgcttg
    18481 cgcaaaaccg ataatccgtt tttcttttgt tacagcaacg agcaatagtt tattcttatc
    18541 atttaggtca gctgccgttt ttgcttttac gaatgattct ggatgatcag taatcagctg
    18601 attgatttga ggtagatctt cttttactgc ccaccgtatt tccatcattt ctcacttttc
    18661 tttcgctcat ttttatctcc actaaaaaaa tcgactgcgg caattgcatc tatccagctt
    18721 tgtgcaatga tctcatgtcc gccaatcgtt gggtggaccc catcttctct tgtatatttc
    18781 gaaaaaccat aagcaatccc taaagcattg aatatgccat ctaaaggaac cagcaatgcg
    18841 ccatattcct tagcaagttt acgaacaatt tggattttag ggtctaagtc tttacgccag
    18901 ccatgacggt cgtcggttat aggaagtaca aacggctcca tcaagatgat gttgcagatt
    18961 cccgcttttt gaagttcatt tagtaatttt cgataaacgg attcaaattg ctgagcagat
    19021 ttctcagttc cgaatatagc ctgatcgcta gtattatgcc aggtatcatt gattccgatc
    19081 aaaatcgata catagtcagg ttgtaaagct aaacaatctt ctcgccagcg tacttgcaga
    19141 tctgcaattt tgtctcctcc tatcccacgg ttcatgcatt caaattccat cgttggatat
    19201 tgggagatta gttttccggc aatcaaaagt ggataacctt tacctagatc agttgcttct
    19261 gctcggtctc ttcctgcgtc tgtaatgctg tcaccgataa ataatatacg ttttttcatt
    19321 tgtcaaaaac tcctttttga ataaaacgat gattttcttc cttatattat cccataagcc
    19381 ctatcaagac gaaagagaaa atatatgtat aaaaaatgac accagcctag tcccttcaaa
    19441 caattgaatg aacgctcatc aagtgtctag ttttttatgc tatttttttc ttaacgtgct
    19501 ggattcccct tgattccttc attgtttggt ccagagagat cgatcacacc taagcttccg
    19561 tcaatcagac cacttgatga cgccatcaga acatatattt ataggctcat ctaagacttc
    19621 cagtaaatgt tcctttacta atatctcttc tagattgctt gggatagatt ggccataact
    19681 ttatctggtc cgatgatttc aagggtgaat tgaacttaca tatggatcat tgcatgctga
    19741 aaatacggac ctactccatc ttcgaatgtt actattagtt tgttttcagg tgctagatgc
    19801 tgctttattt caacagcaga agcatcatca aatcagattt ccatttgaat aaccttcttt
    19861 gtatatgttt gattgattgc gatttcgtta tagaaccttg tgaatcaaat agcaatttct
    19921 tttgctttct tctcttatct gctggaaact tcctttatcc cgtatttaaa aaaaaagaac
    19981 tgcgagagtt cgcagttctt ttttttaaat aaacgttgct ctccggccaa ctccttggtc
    20041 acaagtcaca atagtcatca aacatgaaaa aatgtttttt tgaaattact ccttgtgact
    20101 cggagctgaa cggcagacgc acaacctctt gatataaacg gtgttcaatc agctgaccct
    20161 cgatattagc tcaaaaaaca gtaaaatatg agaagctatt tttccgtttt gtttctctaa
    20221 tactcaggtc aaggcactga tttcacaacc tcttatggat aaattcggtt caacaaacgt
    20281 gggaatggaa tagcttcacg cacatggtcg ataccagcaa tccaagtgac tgttcgttca
    20341 aggcctagac caaaaccaga gtgcggaaca gaaccgtatt tacgtagatc aagataccat
    20401 gaatattctt tttcatccaa accgtgatta cggatttgtt ctagcaagaa atcatagtct
    20461 gttgcccgtt cgcttccacc aataatttca ccgtaacctt ctggtgcgat catatctgca
    20521 cagatcacca cgtcttctct tgttggatgt ggtttcatgt agaatggctt gatggctttt
    20581 ggatagttta ggataaagac aggctgttcg aatgaattcg caatgaacgt ttcatgaggt
    20641 gaaccaaagt cttcgcccca ttcgatgtca tcaaatccat ttttcttcag taattcgatc
    20701 gcatcatcat aagtaatacg agggaatggc aattttgtat aagtttttaa gacttctttg
    20761 tctcgtccta gtacatctaa tgcgtattcg caatgatcta aaacattttg tactaagaat
    20821 gctacatatt gttcctgtac ttctaaactt tcttcttgat gagtgaaagc catttctggt
    20881 tcgatcatcc agaattcgat caaatgacgt cgtgtttttg atttttcagc acggaaagtc
    20941 ggaccgaatg aaaagacttt tccgaaagcc atagcagcag cttccatgta taattggcca
    21001 ctttgagata aataagcttt ttgatcaaag taatttgttt cgaatagatc cgtagtgcct
    21061 tctggtgcag aaccagtcaa gatcggcgga tcgattttga caaaatgatg ttgattaaag
    21121 aattcatacg ttgcacgaat gatttcattt ctgatctgca taatcgcatg ttgtcttgaa
    21181 gaacgcagcc ataaatgacg atgatccatc aagaagtctg ttccgtgttc ttttggtgtg
    21241 atcggataat cgtggctctc gcttaccact tccacatttg taattccgat ttcataacca
    21301 aattttgaac gactgtcttc acggatttct ccagttacta acacggatgt ttcttggtgt
    21361 agatttttag ctgtttggaa aacttcttcc ggcacttcgc tttttacaac tacaccttgg
    21421 aaaaaggctg ttccgtctct taattgcaaa aatgcgattt ttccgcttga tcgcttattt
    21481 gcgacccaag cgccgattgt tactgtttct cccacatggt ttttcgaatc gatgatttga
    21541 atttgttcca cgtatgactc tccttttata ttccttgaat ttcttcagga ttaattttga
    21601 cttttactct caataaattg cttgattctg cgtaccgctt cttctaacgt agcgcgatca
    21661 gtggcataac tcatgcggac attttctggt gccccaaagc cttcgccagt cacaagcgct
    21721 acccctgttt cttctaaaag atcttctacc cattgtgtga cattttcata tttacacatg
    21781 cgtagcgttt cttttacatt aggaaaaaga taaaaagctc cttgtggctt ttcaagagaa
    21841 acacccggca gttctgccac aagcggatat aacgtattca ggcgttcttc aaaggcgttc
    21901 cgcatttctt ccactgtatc ttgctcgcct ttcaatgctt caacagctgc atattgactg
    21961 actgctgtcg gattactggt tgactgggaa gcaacagcaa tcattccatt gatgatctca
    22021 gaattcccca ccgcataacc aatgcgccag cctgtcatcg catatgtttt agacacgcca
    22081 ttgatgatga tggtctgatt tttgattgct tctgaaatcg ttgcaattgg agtaaactca
    22141 ttgccattat aaactaaacg gccataaata tcatcagcaa ctatcaaaat atcatttttt
    22201 actgcccatt caccgattgc ctgcagttca ttcttgctat aaatcattcc agtcggattc
    22261 gatggagaat tgatgattac ggcttttgtt ttttctgaac gtgcttgttc tagctgctca
    22321 acagtaactt tccagctatt ggattcttcc cctttcacaa agacaggaag tccttctgcc
    22381 agttttactt gctcaccgta actgacccag taaggcacag gaatgatgac ttcatcttgc
    22441 gggtcaagga tcgtttggaa aagggtatac aaagcaaatt tcgctccgtc tgttacgact
    22501 gtttgtgaag gttcatagac aagtccatag tttttcttca gataatcaac gattgcctgt
    22561 cgaagctctg ggattcctgc tgttggtgta taatagctag ctttcccatt gcggattgct
    22621 tcgattgctg cttcttggat gttttctggt gtcgcaaaat ccggttctcc cacagtcaaa
    22681 ctcagcacat ctctgccttt tgcttttaat gccttggcct ttgccgcagc tgccaaagtg
    22741 acagacggtt ctaatcgttt tgttcttttt gataactcca tcccattcat tcctttacct
    22801 tttaaattcc cttgattgtc ttgacttcat ctccagattc aaagccaatc aggtaataat
    22861 tcagtttccc atttgtttct tttcctacta cttcccacac aggctgatca tcatacatcc
    22921 ctaaagcagc tttttcgatt tggatttcag gatgggccgc agcaattgtc tgttttgctt
    22981 cgttttcagt cagtcccttt gtttgatcca atacttttac tttttcacca gatttaggaa
    23041 tgataacggc gatttctttt cctttatcat tcgtacccgt caatgaaaag tacgttttct
    23101 ttcgagtaaa ccagtaaaaa tgatcggctt cctcgagatt tgcatatttc tttgcgattt
    23161 ctactgcttc ttttttcgcc tggaccgccg gacgactgga acgcaagtaa aaaatcgagc
    23221 tgatcacgat gaccgctaat aaaaatacga tcaagccaag caacacgcga ttaatcgttt
    23281 tttcctttgt ctgttttttc ttcaagggtc gtcgctcctt tatcttcatt ccttttcatt
    23341 atagcagatt ttaatgatga aaaaagacgg cacctttaac ttccttcttc atttttcttt
    23401 aagaattcat ggatatcctg atagatttca tctatagcta gctcttttat cggtactttt
    23461 ttcggtaatg catggatgat tcgtgcacca tattttgcag tgactaatct ccgatccaaa
    23521 ataagtaata cgccacgatc tgtctccgtc cttatcaaac gacctaatgc ttggcgtaac
    23581 ttcaaagctg ctttaggcac ggattcctga taaaaaggat tgatcccctc tgattccaga
    23641 taagcattcc tagctttgac catcggacgt aacgggttgt cgaagggtaa acgtgtgact
    23701 acaacaatct gcaaagcatc tccaggtaaa tctacgcctt cccaaaagct gtcagcacca
    23761 aataagacgc ttttttctga caaaatgaag cgtttcaaaa gtttttcccg actcccgcca
    23821 aatccttgag caagaatttc acgtcctttt tctagcatag acaaatgcat tttctgatat
    23881 accttttgca aaacatcatg agaagtgaat aaaacaagga tcggccgatc ttcttgttca
    23941 atcaatcctg tgagtgctgc ttctaaataa tcgatatatg cttgattgct gacttgatgg
    24001 actgcaggtg cttctttcgg cacatagatt cttgcctgtt tctcataatc ataaggagaa
    24061 gcaatcactt taagaggtgc tgcttcaatg ccaagtcgtt tagagagata ctgtcgatta
    24121 ggtccgatct tcagtgttcc tccaatataa agaatatggt catatcgagg ataccacaca
    24181 gttgacggca acatagctgc ttgaaaatca aggagatgaa aaatcatctg ctgtgctgta
    24241 ttcccgggaa cgaaccaatg gatcaaatga tctcgccagt cttctagcca ttggtccatc
    24301 agcaagtatt gcttgtctaa ttgatcaaat aagctatata aacttgctaa atcgatccgc
    24361 atctggttgg tccaactttc ttggataatc tcaaaagtca agcgcatctg tgattgtagt
    24421 tgttggatct cttgataaaa aagttgcaga tgatccattg ctgttccaga ttccagtggc
    24481 agctgatcca acatttcttt tgtcatcatg acttcttctg gatattgtcc gttaggttgc
    24541 ttgctgctta ctaagttcat taatccctct gccacgcaaa tctgcgcttc gcatagtgca
    24601 gagagttctt ctcgataaat cgacaaccag cgcaaggctt catgttcctt cggcaaataa
    24661 ctttgtatgc ggtcaaataa ctgattcggc tcttccagtt gctgaatttg tttatggaag
    24721 tagttaatac ttagtcgttt gtcatttact ttttcagcaa tttccggcaa atgatgcact
    24781 tcatcaatca acaaataagg actagctgga agcaatggtt cttttcgctg tgtttcttgt
    24841 actaaaaatg catggttgac tatcaaaaaa ttgctttgtt tgattttctt ttgcaagaaa
    24901 cgcacaaagt cttgtttgta aaatgactga ttccttgcta aaaacccagt gcctctgtgc
    24961 gcgacatctg caaagaatgc atggttcaga ttggttaaat gtaattcatc caaatcaccg
    25021 gtcttcgttt gtgttagcca aactaagaca gccatttggt acaaagcata ttgtttttgt
    25081 cccggcggtt ccacaagtgt acctttaaaa cgctgtaaat caatatagtg gcgatagctt
    25141 ttgataatca caggttgtat cggctgatct agcaattgat tcagtaatgg aatgtctttt
    25201 tccatgatct gctgctgcaa aacaagcgaa acggtgctga tgacagctgg tttttcaggt
    25261 gtagccaaat aactcaaagg caacagataa ccaatcgttt tgcccattcc tgtagctgct
    25321 tcgatcatca aatcttttga ttcatctttt gtgaaatggt cgtagacagc attcatcagc
    25381 ctgttctgtt cttttcgata catcagtgtt ttaccaaaca ttttttcttt tgcttgtttt
    25441 tttcttggat atgtccgctc gccataatgg acggatgtga atagctccac ttcttttttc
    25501 tgcaaagcta gcccatcgat gatttctaat gagagatcaa ggggctccgg gttttctttc
    25561 atttcttcaa gcacgtggta aatgaaacga ctcgtatcca ttcctgtatg gctacttaat
    25621 cgtgcaatct gctctaatgt gatgatcggc aatttcctca ttcgttcttc gatcagtaaa
    25681 agaagctgac ctgtcacttc ggcatcacta tccgcctgat gaggattctc atgaataaag
    25741 ccaaggcttt ctgacaaatc agctaagcga aaacttggtt ccgtcggcaa gaaaatttgt
    25801 gcaagttcta cggtatcgat gcccggtatc ttcaaactag gaagtccgca tcgcatgagt
    25861 tcatggttca aaaaattata atcaaaataa atattatgcg cgacaaatat ggtgtctgcc
    25921 aaaagattgt aaatcgtttg ggcgacatct tcaaaataag gcgctttttg gacgcgttga
    25981 ttcgtgatcc ctgttaaatg ctggatttgt tttgaaatct tccgccctgg atgaatatcg
    26041 atcgaaaaac gtgaagtgat ccgaccagct tcaatcatga cgcagccaaa ttggatgatc
    26101 cgatcttctt ttggattcgt cccagtcgtc tcgatatcaa cgactgcata agttcttggt
    26161 ttcaaaatat cttcctcttc tcctgcctct aatgttcttt tgaaattata ctacaaagtg
    26221 cagaaaaata ctctcatcgc tttttaagga aaaaaagaca aaaaagccca gacacataaa
    26281 tgtctggact tttattttta atataggtaa attaagcttt agtaacgttt gttgcttgag
    26341 gtccgcgttg accttcttca acgtcgaaag ttactgcttg gccttcttct aaagttttga
    26401 atccgtcgcc ttggatagct gagaaatgaa cgaatacatc gtttccgttt tctgcagtga
    26461 tgaatccaaa tcctttttct gagttaaacc attttactgt acctgtttcc ataataaaaa
    26521 tcctccttgt gtatcaacac atattttgca attacttgaa tggtgtatat ctggaagatg
    26581 tttatttgaa acaagctacc acagattacc gaacaaaaac tacattctta ctatatcata
    26641 ccgtttacaa aattgccaga gtttttcctt atttttttgt atttattctc ttatttagaa
    26701 gatatatttg caaataaacg aacgttttct cgaaatcacc cctataagat tcagaaaatt
    26761 tctatttgct attttcactt ttgcaaaaaa tttcaattta ttatctgaaa aaggatgaac
    26821 gaactttgga attctctaat gatccacatc attttttaac tgataaaaat tagtatgcgc
    26881 atctatcccc ctctcaactg caaggttcgt ttttttgaga aaagtatctt cccctcgcaa
    26941 agccaatggg actaaagcac aaaaataatg agcgagagac aaaagctagc ttttgtctct
    27001 cgctcaagat gaatccaacg gctgaaacag aggaaactcc ttctctcgga gctatacact
    27061 tctatcaacg cctgaccatt catctacttt ggtagttttc actcaataac gtaagatcgc
    27121 aactaaactc ttttcgtctg gtgcatcttt ttgttcaagg ataaagacct gaccaccgtt
    27181 cgtgatcaca ttatctgcta gttcattcag cacctgacgg cggtcatatt ctgtttctgg
    27241 atcttcgcca aaaccatctg ctaaattaga tgtagcaatg aataaatggg atattttccc
    27301 ttcttttgct gccggtataa tatcaacgag ttgatcaatg aactttttat caagcaactt
    27361 attgtagtca cttgcttctt tgacagctaa ttcttgcgat agtttttttg cgcctgcttc
    27421 aatttctttt aatgaaagtt gtgcaggtga agctgagatc gcaacatcgg aacgataata
    27481 agggttcttc gccacttttt tgaacatcgt ctgattttct ggtaatgcaa ataagaagat
    27541 aggccatttc tctggatttt caaatgtatc tttcaaatag tcatcgactg cttgataata
    27601 attgacccaa tcaatttcga cttcttcatc tttaggattg actccatgga acgtcgtacc
    27661 ttcactacca tttgatgcgt agttcaaccc gcctcctgtt agttcagatc ctaaagcagt
    27721 gacgacatct cttggtgcat cttcaggcaa agccacttct ttgacttgtt tattcgttac
    27781 ttcgtacaat ttcatcgaat cacgattcaa tcctaataaa tagtatgaat agttgaaatg
    27841 tccatttttg atgatcgcta ataaataagg tagatcactc acataatact ggtcatctac
    27901 aggaataccc aaacggtgga taaacacttg ttctggtgat actatgacag ctacactcgt
    27961 tgtggcacta cgccagaaag acggatcgtc caacaaacta gtaatttttt cttggaatgg
    28021 agaccaatca tgctcagagt atttcttttc aaagcgcttt ttcgcttcct ttgcaaaatt
    28081 cttgaatttg atttgatctt tttctacgtc ttgatgcgca acatgggtgt ttaacataat
    28141 cgtgacaaac ggtcctttgg taccttctga agtcaattgt gataataatt ctttttcttt
    28201 ttttgccata aacgtacccc tcctaaaaaa atcatttctt tgtatctgta aataaagtct
    28261 accacatccc attttatcaa tacaataaaa cgaatcccta ccagagaaat acctgatcga
    28321 tttttagttg tgttgtccta cttcatattt ttttcatact ttcatcaatt atatgctcat
    28381 ttccttaaca gtagcttcaa actctcttgc tatactataa aaaaacaaaa aagaacgagg
    28441 tgatcttatg caattattca tgatttttag tttggtcgca ctctcagcag gcgccctttt
    28501 tgcagcatca cgcctaaccg aacccgaagt tcagccagtc aaagttcgtg tcaatgaacc
    28561 caaaagataa ctttctatga tt
//
LOCUS       DASJSZ010000031        27715 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.031, whole genome shotgun sequence.
ACCESSION   DASJSZ010000031 DASJSZ010000000
VERSION     DASJSZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.031"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(210..1154)
                     /locus_tag="U4B03_001259"
     CDS             complement(210..1154)
                     /locus_tag="U4B03_001259"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN2068121.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1450..2394)
                     /locus_tag="U4B03_001260"
     CDS             complement(1450..2394)
                     /locus_tag="U4B03_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2068122.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2615..3829
                     /locus_tag="U4B03_001261"
     CDS             2615..3829
                     /locus_tag="U4B03_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2068123.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3851..5866
                     /locus_tag="U4B03_001262"
     CDS             3851..5866
                     /locus_tag="U4B03_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEN2068124.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5949..7322)
                     /locus_tag="U4B03_001263"
     CDS             complement(5949..7322)
                     /locus_tag="U4B03_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN2068125.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7348..7632)
                     /locus_tag="U4B03_001264"
     CDS             complement(7348..7632)
                     /locus_tag="U4B03_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2068126.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7634..9649)
                     /locus_tag="U4B03_001265"
     CDS             complement(7634..9649)
                     /locus_tag="U4B03_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2068127.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9828..10133)
                     /locus_tag="U4B03_001266"
     CDS             complement(9828..10133)
                     /locus_tag="U4B03_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEN2068128.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10222..11253)
                     /locus_tag="U4B03_001267"
     CDS             complement(10222..11253)
                     /locus_tag="U4B03_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2068129.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11460..12335
                     /locus_tag="U4B03_001268"
     CDS             11460..12335
                     /locus_tag="U4B03_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2068130.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12487..13329)
                     /locus_tag="U4B03_001269"
     CDS             complement(12487..13329)
                     /locus_tag="U4B03_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2068131.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13421..15298)
                     /locus_tag="U4B03_001270"
     CDS             complement(13421..15298)
                     /locus_tag="U4B03_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2068132.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15295..16716)
                     /locus_tag="U4B03_001271"
     CDS             complement(15295..16716)
                     /locus_tag="U4B03_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2068133.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16719..20108)
                     /locus_tag="U4B03_001272"
     CDS             complement(16719..20108)
                     /locus_tag="U4B03_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN2068134.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20365..21756
                     /locus_tag="U4B03_001273"
     CDS             20365..21756
                     /locus_tag="U4B03_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2068135.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21829..22476)
                     /locus_tag="U4B03_001274"
     CDS             complement(21829..22476)
                     /locus_tag="U4B03_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN2068136.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22484..23170)
                     /locus_tag="U4B03_001275"
     CDS             complement(22484..23170)
                     /locus_tag="U4B03_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068137.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23176..24249)
                     /locus_tag="U4B03_001276"
     CDS             complement(23176..24249)
                     /locus_tag="U4B03_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068138.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24471..25145
                     /locus_tag="U4B03_001277"
     CDS             24471..25145
                     /locus_tag="U4B03_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2068139.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25150..26490
                     /locus_tag="U4B03_001278"
     CDS             25150..26490
                     /locus_tag="U4B03_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2068140.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26639..27625)
                     /locus_tag="U4B03_001279"
     CDS             complement(26639..27625)
                     /locus_tag="U4B03_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEN2068141.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8077 a         5243 c         5029 g         9366 t
ORIGIN      
        1 attaaccaca gataaacatt gataacgatt cgcttattgt aatcaacaga ttaaatttca
       61 tatgttcata tgtacggata cattttcgga ccgattaatt ttaaccattc tatttatttg
      121 cttacctatt agatatcaat aaaaaactgc ttaacagcta tttcagctgt taagcagttt
      181 taggattttt agttcatatt cattgtttct tataatacag tgttcaatac ttctcttaaa
      241 gcacttgttg atttgctgaa cattgctttt tcttcttcgt tgattgataa ttcaacaact
      301 tcacgaacac cattttcgtc aacgattgat ggcacacctg taaagatatc tttttcgtcg
      361 tattcaccag ttaaataagc agatacaggc aatacagctt gttcgttatt caagatagct
      421 tttacgatac gtgcagtact cataccaata ccatagtaag ttgctttttt acgatcaatg
      481 atctcataag cagcattacg gactttatca gcaatcacat ctaattcatc ttgggcaata
      541 cgatggtctt tttctacaat ttcaaacact ggtttaccac cgacagttgt atgtgaccat
      601 gcagcaactt ctgaatcacc atgttcacct aagatatagc cgtggacgct gcgtgggtca
      661 acttttaatt tcaatgcgat ttctttacgg aaacgagttg tatccaatgt tgttccagta
      721 ccgataacac gtgatgttgg cagaccagat tcgttccaag cgatatatgt caaaatatct
      781 actgggtttg aagcaacaac gataataccg tcgaatcctg atcccatgat ttcttttacg
      841 atttggcgca tgattgacgc atttgtttta actaaatcta aacgagattg accaggtttt
      901 tggttgatac cagctgtgat aacaacgata tcagcatctt tacattcttc atagccacct
      961 gaccatacag ccacattttc atcgccccaa gccataccat ctaaaaggtc caatgcttcc
     1021 ccttctgctt tttcttgatt cacatcgatc aatactaatt cattagagat tccttggttg
     1081 atcattgcat aagcgatact tgttccaaca aaacctgtac cgacgattac tactttacga
     1141 cttgtttttt tcatgaataa acagctcctt ttcataacta tttgttactt tcatgaaaag
     1201 tatagtcttg tttattcatt ttatcaacat gttaattata atagttgtta ttataatttt
     1261 tgatggaaac gaattcctga atacgcataa cagataaatg acaaatgttt acacctatat
     1321 cccctgataa tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa
     1381 cgatataata agacagcaat cgcacctaaa acaattttcc ttcatatgct ttagtgaaag
     1441 attttcttct taatgtttat ttctccattc attgggagta catgttgaat acttcttgaa
     1501 taatttgaat aaagaagtcg tttgtttata tccgatacga ttagcgattt cttccaacga
     1561 aaaactcgtt tcttgaatca gttccaacgc aaagtcgaat tttttcttta gaaccagttc
     1621 ttgaaaggaa gcatcaaaat acattttcaa tttattacta agatagaccg gattgtagcc
     1681 aaaatgagct gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga tataatctaa
     1741 tactctaagc atctcatttt ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa
     1801 taaatgagtc aattcgatca gtaatagtga tgtatagaga tttaacgaac ggatcttatg
     1861 actgtatgta tcaaaataaa tcaacagcat attttcaatc aaatgcgtca aacgacttgt
     1921 ttttccgccg cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg atgcatttat
     1981 caaaaaagaa gaaagtagat tatttgtgct tgccaaatga ttcaatatct ctgtagataa
     2041 ggtttcttct tttaataaga tgttgattaa aatatcctct tgccctgtgt attcgattct
     2101 ttgagtagct tctttatcaa tgatcaccac gtcactttct tccatctcta tcctttctcc
     2161 attaataatt tttaaacatg taccgctata catataattg atttcaacaa aatcatgtgt
     2221 atgctcggga acttccgcat atcgacggtg tttactgata tgaatatttc cctgttgaaa
     2281 tagatcccct cggtggattt ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt
     2341 gatattcatt ccttgttcac gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa
     2401 tgtctaattc agctagttcc aattgactcc ctcctttttg taagcggttt ctttcgctta
     2461 ttagttcacg ctgtactgct attatatgca aagctatttt tggtttcata aagactttct
     2521 atggttggga catgaacttc ctgtgattcc gctattgaga gaataaccac ttcatactat
     2581 atttttgata agcgcttaca aaaatgaaag gaacatgctt atgaataaaa agatgatgaa
     2641 attttctctg ctgtcagtct ctcttatttt gacttctgct ggtgcagtca cgtctgcaat
     2701 tcccagtatg tcagctactt atgccacgcg ttctttatct gctgttgaat tattaacgac
     2761 tgctccctct tttatggtca ctatttttgt tttgattagt agttttattg ctaggaaaat
     2821 cggacaaaaa caaactgtga tcatcggatt agtgattgca tcgatttgtg gtatcctccc
     2881 cctcttctca caaagttaca gtattgtctt agcttcaaga gctggactag gtattggctt
     2941 tggattgatc aattctttgg cagtcagcat gattgctgcc ttttatcacg gagatgaacg
     3001 agcctctatg attggttttc aaagtgcatt tcaaggattt ggtgcagctt taatgacatt
     3061 tgttgcagga cagctagtac agttcggttg gcaatatacc tttttagtct acgccatcag
     3121 cttacctatt cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca
     3181 atcgcatggc acagccccaa caagacaagt aatcaaaacg aatcctaaaa tgattagtta
     3241 tggtattttt ctcttgctgg tcatcgtggt atataatgct gtatcgatta aattagccac
     3301 agtcctgacg acttattctc taggaaacga aactaatgca gcgaatatct taacaatcat
     3361 gcaattggca agtatggctg ccggagtttg ttttggctgg cttcagacaa aagcacatcg
     3421 ctatatgctt acactttctt tactactgat gggaatcggc tttgtattga ttgccactgt
     3481 gataaacctg tatgtgattg ggtttggtgc gatcctaaca ggaatttctt tttcgttatt
     3541 cattccttac ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa
     3601 tacttcaatc ttattggttg gcgctaattt aggtactttc attgcaccat atggtttgct
     3661 ctttctctct tttgcttctc ttgggagtaa aacacttgct gttttcgtca atggcagtat
     3721 cttgctgttg attagcgcgc ttattagcct tctagtagta cacaggacga atagaaagac
     3781 agtgataaga gtagatgaag aaataaaaga acaattgaca acaaaataaa tttgatggag
     3841 gtcgtgtaaa atgacaaatg aaaaacataa agatagatgg ctttggtatc ctggagactt
     3901 tgagatccgc catggactgc tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc
     3961 agcgtactgg tacatggatg attgccatcg gaacgtcaag tttaagagat attactttct
     4021 tgatcaacct tccatgttta aagtgacgat ccaaggagtt gggtatgtag aaattaatgg
     4081 acaaaaacat ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa aaattcgtat
     4141 ctttgtagga catacatcag ggctaccagc gatgtttatc gagggagacg aagtaaaatc
     4201 agatattggc tggacagcaa gtaattttat tgaagaatat ccagctggct ggagtccgct
     4261 ttatgtcgat atcgcaaaag atccaaatca aatctattat caaaaagaat gcgtccatcc
     4321 agtcactgaa acggagattg gcggaggaat cttattcgat tttgggcgag cagtgaatgg
     4381 ctgtgtagaa ctaatatctc tggatgacca tatcgaatct atcactctat gttatggaga
     4441 gtccgatgta gaagctatgg atatcgatta ttgctactat aagcaaatag gaatgacaag
     4501 aaaaatgaaa gctcgtaaac gcgcttttcg ctatttgttt ataccagagg tttctactgg
     4561 aatggttcaa cttcaagttt ttcatgaatt tttatctctt tcgaaacaag ctgctttttc
     4621 aagcgattct tctttactga atcagatctg gtcagtatca gaagaagcct tcaagttatg
     4681 tagtgatttg ttttttatcg acggtatcaa acgagatcgc tggatttggt ctggagatgc
     4741 gtatcaatca ttcatgatca atcaatatct tttttttgac gaggaaatca ataaacgaac
     4801 gatattagca ttaagaggac aaaatgaaat caagcagcat atgaatacta tcgttgacta
     4861 ttcgatcttg tggttgattg gtatcgaaaa ccactatatg atgacgaaag actatgaatt
     4921 tttacgcatc gtctacccta agatgcaatc attaatgaga tacttgatgg agcaaacgaa
     4981 tgaattaggg tttatttatg ggagagcaaa agattggata tttattgatt ggtctgaaat
     5041 ggataaagaa ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct atcattccat
     5101 gatcacttgc ggagatatct tagctgaaac atatggagag aacgaaaatt atcatactca
     5161 tctctatcaa gaaaaatatc aactattgaa acaaaaccta tttgactact tttgggatga
     5221 agaacttggc gcttttatcg attgctatga atctgggaga agaaatgtga cgcgtcatgc
     5281 gaatattttc gctgttttat tcgattttgc taacaaagac caacagcaat cgatcttaaa
     5341 gaatgtctta ttgaatgaca ctatcacaca gattactact ccatatttca aattctatga
     5401 acaagatgct ttatgcaaac tcggacagac gaatatcgtc taccaaacga ttcttgatta
     5461 ttggggaggg atgttatctc atgatgcggt aactttctgg gaagaatacg accctactca
     5521 aacaggaaat gaaaagtaca gtatgtatgg agatccttat ggaaaaagct tatgccatgc
     5581 atggggagca agcccaatct atcttttagg acgctatttt atcggtttgt atccaacaaa
     5641 gccaggttat gaagaattta tgatacaacc taatctagat tcatttgaaa cactgaattg
     5701 tacgctacct atcaaagatg gaaagatttc gcttgaaaaa acaaaagata agatcgtgat
     5761 tacttctgat agaacaggcg gaacagtgaa aatgaatggt aaaagttatc ctcttctagc
     5821 aaaacagcca cttgtcttaa caagctattc aaaaataaat gcataaaaca aaaaactgct
     5881 tgcgacatat cagaagactt taaatcttct tctgatattg tccaagcaat ttttattttg
     5941 aagtttgctt aattctgttc tttagaaaca atgtttgttc tttttccggc aaaaagaaag
     6001 ctagataata cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg
     6061 tattctccta gtttcccaag tacaagtcct ggtactagat aatcagtatc ggcaaatgta
     6121 gaagaagcga ttttaagatc tcctaatact ggaaggataa ataatggcag ccaactaatc
     6181 aataagctat tgaaagcaga acctaatacc gcccctcttc taccgccagt cgagttgccg
     6241 aagacaccag aagcagctcc gcagaagaaa tgtcctacta ccccaggaat gataactgta
     6301 gtttgcaagc ctatcatagc aaacatggat agtgttccta caaagaagct aacaaaaaag
     6361 ccaattaata cagcattagg tgcatatgga aaaacgatag gaacatctaa tgctggttta
     6421 gaatcgggta cgagtttttt agcaatccct tggaatgctg gaacgatttc tgccaacacc
     6481 attcgaaccc cttgaaggac gataacaaac ccagctgtga atgttccagc ttggatcagt
     6541 gcaaagatta cagcgttgtc tccattgctt agttctcttt caacaaatcc aggaccagct
     6601 aacaaagcaa gtgcgacata tacgatcgac atcaagagca tgatcgatac agtagaatct
     6661 cgcaaaaagc tgaaactttt aggaatcttt atgtcctctg tagaacggtc tttgtttcca
     6721 aaaaactttc cgatccatcc actagaagca taaccgatat ttccggtatg ccctaacgca
     6781 acttgattgt ttccagtgat gttcttcata aacggttgac aaagagcagg cgtaatagtt
     6841 aatagtgttc cttcgaagac cccgcctaat aagatcatcc acaaatttgc agtaaacccc
     6901 gcgcttacta aaatgactgc tgttagacaa gaaacataaa gcattgcttg acctgtaaga
     6961 aaaatgaatt tatatcttgt gaatctagca agtaaaatat tcacgatcat ccctactagc
     7021 atgatcaaag cagtaggtgt accgtattgt tgcaaagcaa tcgccactac tgcttcattg
     7081 ctaggaacta ctccttgagt atgcagagct gtttggaaca tcgaagcaaa aggggttagt
     7141 gaattactga ttaaaccagc accgccgatc agtactagga atccgacaaa tgttttgata
     7201 gttccttgga tcacttctga aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc
     7261 aaagaaacga ggatggaggg ctgactgacg attgacacaa acaaattcaa caatttatcc
     7321 attgatagtt cctcccttga atcattttta ttcttttgac aagatcttcg ttactttttc
     7381 ttttacttct tcgctgtcaa tgatcgattg caaaggtacg agcttttcat caggtataga
     7441 agttagcgta tctttcaaag aatgctcaat gaagaaataa tcagccatat cgctagatac
     7501 acttccaaca tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag
     7561 tgcttgtttg atgttcatct ctaccataaa acttgttcct aatccacttt gacatactgc
     7621 aaaaattttc atatcattcg tctccttttc ttatgatcgc taatatttct tcttttgttg
     7681 atgcgtgtaa taaatcttga agattttgtt tattagaaag tagctttgtt aaatttgcca
     7741 aagcccgaag atgtgcctca ttatcgatag cagctaaaca gatgaacaaa ctaatgggat
     7801 gggctggatt atcaagcaga tccacaggtt gttgaagttt cagcaaagac atccctactt
     7861 cgtttacgcc atcttctgga cgagcatgag gtaatgcgat gtgatcgcca atatggataa
     7921 acgcaccata ctgctccacg cgctcgatca tagctttgat ataactttct tctattttat
     7981 tttctgataa tagcggctgt gcggcaaatg tgattgcttc ttgccagttt tttggttgat
     8041 ccgataattg gatcatttct tctgttaata attcagatag cataggacgt tgatcctcct
     8101 tcttttctaa aagtttgttc attttacgat ttaatacttg atagagcttt tcttcgtcta
     8161 cgccttcttt taattcgata taaggcagta gcgccgacaa caattcttgg gcagaaggca
     8221 ttgaaaatcc tggtatcagc caatctcttt gtacttgatt gatcagctga tttttttcca
     8281 agtcagtcat gattggatgc agaacataga gagacttttt cttatcagta agtgtgagag
     8341 gtacagtaga aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg
     8401 aattagtctc tgtaaaaaag atcatcggga ataattgctg aagttcactt tttaagatca
     8461 atgaagagct aatgccactt ggacagacga tcacggctct tatttttcta tttctttctt
     8521 ctgcgtccgc ttttctgatc tctccgccaa atagtatagt gaaaaaacca atctcttctt
     8581 cagggatgct cttccctatt cgtctttcta acggaagtaa tgcttttctt gtcatttcgt
     8641 agatggaagc atattgatgc ttaatttgat cgatcatcac attaggtaaa taaaaagaat
     8701 aggaaatacg aaaataggca gggaccaagt gataaaagac attcatcaat aattcacgaa
     8761 acgaagcaaa ttctacggcc atcaggaatt ccatgcgatg gatgacttct gaagaacatt
     8821 ccagcaaaaa atcaagtgca ggctcctgga catctccttc aacgatcgat gctaagcaaa
     8881 gagagaaaaa tataatctcc gaattttgat gttcggaata gccaaggcca gtcaatactt
     8941 gtcgactaat gtcagtgact gcaagagaag ccaacagttc agcatctttt accagttcca
     9001 tggatgtttt tccaattcga atggttgtca aactcaaata ataagctaat tcatccattc
     9061 gatttggtgc gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa tctctaactt
     9121 tggcgtaata atcaatggaa agggttgcgg ttaaatacgt gagcccaaat cttccagcaa
     9181 gtgactgcat caaacgagca gtgcgatttt ttgcttctct tcttaatatt gcttctgacc
     9241 ctagcagcct aaaaccagtt ttcctagtat acgacagctg acaatcttcc acttctaatt
     9301 catttcttat ctttttgata tctgacagga ctgtcccttt agaaacctga agaaaatctt
     9361 gaaaatgaaa aacagaaaca gacgttgcag tcgaataaac catcaaataa agaagatctc
     9421 ttcgctcttg ttcggtatac acagcttcaa atcgagtaat atccaaaaat ttttctgccc
     9481 agtcgattgc tcggaatttc tttatacgga tacgggtatc atccacatat tcggcaaaag
     9541 gagttaattg tttgatcgcc tcgcttgtat ttacttcgat gatctctgct gctttttcta
     9601 gactgatttc tcttggtgtt ttcaggagat aactaatttt gtagttcaca gcgctgttca
     9661 cctcctactt atattctagt atggtagcgc ttttaaatcg aggggtgttt ggtacaactt
     9721 acagttggat tttttttacg actgtaccga acgatttcta tcgaatggat acagaaaaaa
     9781 agcaggtgac caaatgtctc ctgcttttat gaattgattt cgtccgatca atgatgatca
     9841 tgtttatttt ttgaggtcag ttcttcaaat ttttctttga catcttcaac cgctttttcg
     9901 atatcttttt caaagtgatt gggctcatta ggctttgttt cctcttctac agaatttgaa
     9961 ccagataaag ctgtttcttt attttctgtt tcaccagagt ttgtgccagc taatgattct
    10021 tctttgtctt ctgtttcacc agaattagtt cctgctaaag ccacttcttc ttctgtttta
    10081 ggggccattg aacctcttaa tcgtttgtgt ttttcaccgt cataatttgt catgttggtt
    10141 cctcctagta gttctatact ttttcatcgt acaataagac agtttatgcg tcaaaaaaca
    10201 aacccgattc ttacaaatat gtcatatttt tccttctgat tcttgaatgc ttttgattcc
    10261 ttcttccaaa attttcattt gtaagattgt ttcttcatct ttgatgactt tttctgcgcc
    10321 attaactaat gtctcgtaaa tcccttcgta gacacggctg taatcaccaa cctcggaaat
    10381 tactttttct tcatgataac tcccttgttc atctatatag gtcaatacac cataatgttc
    10441 gggcaaatcg ataccaaagt cttcatgatc aggcatgtaa aacattttca gatgttcttc
    10501 ttgtcgatct tttgtttgtt tcacgaacat cccttttttt ccgtaaacga cgaaacttgg
    10561 acgttctttg atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa
    10621 atccagatcg aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct
    10681 gatatcatct ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc
    10741 atataaatag ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa
    10801 ataatcataa tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt
    10861 ttggactgtc aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc
    10921 tttttctttt gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt
    10981 ctctactagt acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg
    11041 actaggtgta gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg
    11101 atcagtatat tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt
    11161 tttgatattg attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact
    11221 ctttccattg ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt
    11281 tcagtatatc ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc
    11341 agaaaaacac ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat
    11401 caaaaagata gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca
    11461 tgcttattga agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg
    11521 tcgattacat ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa
    11581 aagctacgta tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg
    11641 gatttaaaga attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca
    11701 atcaaacgat tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa
    11761 agaaaatggc ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg
    11821 aagatttaga tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa
    11881 aaggtgattc tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt
    11941 ttttgatcat ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg
    12001 aggattgcgc catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga
    12061 cctatttgaa gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa
    12121 tggccaaaca ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag
    12181 tagcgacttt ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga
    12241 tatattctca aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc
    12301 aaaaaggcct tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag
    12361 aaagatagtg tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt
    12421 tttcaaacct cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag
    12481 gaatagctaa cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag
    12541 gaaaatcagt atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac
    12601 tttctgtact tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag
    12661 taagcgatga ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc
    12721 tttttcgatt ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg
    12781 ataagcgagt gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg
    12841 taagtctgtg aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt
    12901 attttctccc cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag
    12961 cacagtattc gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat
    13021 accgatcgta tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt
    13081 aaagggatct gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat
    13141 gttttcttgg attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg
    13201 agagatgatc tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag
    13261 tatccctatt cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt
    13321 tggtttcatt tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt
    13381 gcgtaatccg ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc
    13441 gtctagcaaa gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat
    13501 agattccctt accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag
    13561 gagaaatcaa ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt
    13621 ttgttaatgg aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt
    13681 ttagacctgt cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt
    13741 tcgccgatac ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt
    13801 cactatcttg atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt
    13861 caccatctac tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt
    13921 gatcatttgt atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg
    13981 ctttttcgtt caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag
    14041 gaatataatt cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat
    14101 ttactgggtc gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat
    14161 cattatcaaa tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt
    14221 tttgcgttcc ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa
    14281 ttttttcacc gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag
    14341 acgtatcatt tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag
    14401 gcgtgattgt ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat
    14461 tatattttcc aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg
    14521 caaagtcatt gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt
    14581 gatcagttgt ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg
    14641 gtgttccttc ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag
    14701 taccgatttt tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat
    14761 ggatagtatc tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct
    14821 catatacagg gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt
    14881 ctttgatcgt gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat
    14941 ttccatcagc atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg
    15001 cttgttttgc tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga
    15061 cgttataaac tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt
    15121 ctttcccgct gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga
    15181 tgacttgagc tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta
    15241 atggtaaaat aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt
    15301 tttcactcct gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc
    15361 aacaacgcct aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt
    15421 ttctttttta ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt
    15481 tgaaatggta aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc
    15541 ttttaatcga taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga
    15601 aatcaggtga agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg
    15661 gaggtaattt cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg
    15721 tttatttgtc tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt
    15781 actgacacca aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc
    15841 tgtactgctt agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa
    15901 cgaagggatc gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc
    15961 tgtcagcttt tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc
    16021 atccgcacta tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt
    16081 atgcaaggaa taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac
    16141 cttctcaaaa ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt
    16201 tgaaagtttt tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt
    16261 ttctttgacg acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc
    16321 ttgatcttta gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt
    16381 tgttgcttct gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc
    16441 ttcttctacc gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac
    16501 ttggaatgtg acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc
    16561 atttggatga tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg
    16621 aaccatttct gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa
    16681 gaagagacac atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt
    16741 ttcaaaaaaa gataccaaat agaaaacgca caaccaatca tccctactag atagattcct
    16801 aaacggccaa ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa
    16861 gaaatctggt tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact
    16921 tgaattgacc catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct
    16981 tctggtgtaa aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag
    17041 gtatctgttt cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg
    17101 aattttgctc cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac
    17161 gcttttagtc gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt
    17221 cccgtgtaaa tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta
    17281 tcatatccgg caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca
    17341 aaactggcaa gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt
    17401 gcttcttttt tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct
    17461 tgcatcgcat acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac
    17521 tggatcgtat catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa
    17581 gcgatcttga tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt
    17641 tctgtcagcg tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt
    17701 ccgtcgccat gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg
    17761 ccaataagat ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg
    17821 aattgtttag tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca
    17881 ggtaattctt tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt
    17941 gataatatct cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt
    18001 tcccacgtat ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa
    18061 gctgcagcat ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca
    18121 gctagattgt tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct
    18181 ttagcagatg gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt
    18241 gtcctgccgt tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt
    18301 ctcacttggt aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg
    18361 atggtatttc cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc
    18421 ttgactgaaa gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt
    18481 ccattgctta cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct
    18541 gttgcatgat cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt
    18601 cgcatacgag actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg
    18661 atgccaagac catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt
    18721 gcgataaacg tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt
    18781 cttgagatta gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa
    18841 taattgctgc ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca
    18901 tccgtcagca aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca
    18961 tctcttagtg ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt
    19021 acttgatttt tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat
    19081 ccttcgcttg agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct
    19141 aaagtatcga caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc
    19201 atacttcctg accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca
    19261 tttccacgag cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac
    19321 tttcgtatcg catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc
    19381 cgatcattag gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta
    19441 ttgccttgat gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa
    19501 gtgccctgat catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca
    19561 gctggcaagg ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat
    19621 gattcttctt ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct
    19681 gtctcatcaa taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg
    19741 taaagtgctg gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt
    19801 tccaacgtaa ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc
    19861 acatcactta agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg
    19921 ttattcgcat ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca
    19981 gctgaaattg tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc
    20041 acaaagtttg ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg
    20101 gtcgtcattt ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat
    20161 taaaataaac aggcgttaca taattttatt tattttaata tgataattat tatacgaagg
    20221 gcgctaaagg gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc
    20281 ttcgattaat gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg
    20341 ttgttggaag gaaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac
    20401 aagtggatga ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta
    20461 aggtatatcg aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa
    20521 aaattggggt atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa
    20581 atgtcgcaaa gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa
    20641 acgattgagt attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg
    20701 aaacaatgca atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt
    20761 cagttaacta gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta
    20821 agttactacg gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt
    20881 atggtcaaga ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct
    20941 ttctttatta tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa
    21001 tggaaagctg gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc
    21061 ttgaaaaatc tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt
    21121 gtcaatacga ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata
    21181 cgaaaaaata tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa
    21241 tatgttgttc aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg
    21301 actcatttga cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt
    21361 ctatatgctg gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg
    21421 atttattatt tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat
    21481 tatacttctg ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat
    21541 attgaagaat atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct
    21601 attactgaag atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat
    21661 caacccattt tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca
    21721 aatgaagaag aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata
    21781 aaccaccctg ataaaaattt caggacaaat acacatagct gtttcttttt attgtattcc
    21841 ttcagcaatc caacgagatt tatagcgaaa gtcttttaac aactcttctt ttgtccaatt
    21901 atttgcaatg agatccgtca aagtgttcat tacgagggtc atggaaaagt ataggaaact
    21961 aactgcttct tccgacgatt gaatggagaa atgattgatt cttaataatt ccagccattt
    22021 ttcgttcatt ggagaatcag ccgatagttc tctacgtttg gttgtctgct gatcatttcg
    22081 ctgggttaac aagtataacc tttgccaata gatactttcg tgatctaagt cgcagcacca
    22141 ctctaaatat ttttcgatcc ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa
    22201 gatatcttga tggactatgt gagaactgct tttgatcgta tacaaataag catcttccaa
    22261 atcttgaaaa tatttataaa aagcaccgcg cgacatcccc atcttgtcaa cgatttctga
    22321 gactttgact tggctgatgt gctgtgagca aaatatctcc agtaataggt catcgattct
    22381 ctgtttcttt gcgttcgata aattgaaaaa agtttctttt ggcatccaca tctctccttt
    22441 atctctgatt atacgtggat agcccttcat tgtcatgaag gattcattcc ttctttttct
    22501 cttcttctgt caagtaccca tcttccattc gataaacgcg atcactccat tgaatcatac
    22561 ggacatcatg agtaaccata atagtcgctt tttgtttctc atgagcttct ttaactagta
    22621 atttgacgac ttcatatgca tgatctgtat ccaaactagc tgttggttcg tctgctaaga
    22681 tcaagcttgg ctcattatat aaagcgcgag caattgccac tctctgtctt tctccaccag
    22741 aaaggtcttt aggataacta tttttaagtg actggatatc taaagattgc agcaaggaat
    22801 cgatttttgt tgaatcttct ttttttgaag taacttggtc aactagttca aattgttcct
    22861 ttaccgttaa aaaaggaatc aaattagagc tttggagaat aaatcctatt tctttaaagc
    22921 gtaaatctgc tcgttttttt tctggaagct cagttagtga gtgaccgttg atcactattt
    22981 ctccggaagt cggtgtttgc aatcctcctg aaatggtaag gaaagtactt ttacctgaac
    23041 cagaaggacc aatgatgctg acgaattctc cttcctttac ataaaaatca attgctttca
    23101 aagctgagac ctttgtatga ccagaaccaa atgttttggt aacttttttc atcgttaata
    23161 tttttttcat ttttattaac ctcctattgc tgtaattggg tctactttgg ttatcgtacg
    23221 tatcgaaaac aatccgccta gcattgcgac aatgatcaaa atcccactat atagcaacca
    23281 ttgtgaccaa aaaacagcaa aaggcatcgc gcttggtaaa atcagactag tcaaataagc
    23341 aaacaaaaga gcaagcagaa cgcctaccac cccaacaata aaggactgtg ctactaaaga
    23401 tttagcgatg aagaaatttt tgatcccttg tgctttcatt actccaaaaa tagcagtctt
    23461 ctgaagtgtc atcacgtaca tgaaaatacc gaccacggca gcaaccacta agaacaaaaa
    23521 gtatatcatc gcatttaagg tcatattttg ggcagaatag ccaggaatac tttcaatgaa
    23581 tgtcccaata gttaataact gtagaccctt cgatgctttt tctttcgaaa ttttagctga
    23641 atcctttatt acgatcccat tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc
    23701 atacttcaaa gcagtccacg tatctaggga cgcatagatg acgggagaaa ctgtgtaata
    23761 agtttcagga aagataccga caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt
    23821 atctccaatt ttatagcctg aatcagccag attttgagag atgatgattt cattctttgt
    23881 tttaaattca tgccctttcg tgagttttgg taataaaaat gcttctttcg ttgtgccgaa
    23941 aactgtaaca ttttctttgt tttttccttt gattgctccg ctgtacaatc cgattggtgc
    24001 tttctctcct ccttcaatgt atttcagatc tcctcttgtt aattgtgaag ctgcaaatac
    24061 ttgatttgca tcctccgaca aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt
    24121 aaactcttct gaaagtccat tcgctagtcc tgacaacatg aatacgacat acgcaatcaa
    24181 gaacataata ccgacgatca agccatatcg taatttagaa tagcgaattt ctttgatagc
    24241 tagaaacatt tgtttttcct ccctatgata gattgatagt gacaaaatgt cacttgttga
    24301 atagtgtaac tctaaagtga caatttgtca cttgtgttgt ttttttctta tcaataaatt
    24361 gtctaagaca tctttcgata aaaccttaac atgagtcata aaaaatttat ttaagcctta
    24421 tttatgcttt ccatgataaa ctaaagtgac caaatcaatg aggtgggaaa atgattaaac
    24481 tattagtagt agaagacgat aaagtattat ccaataatat aaaagaaata ctcgctgcaa
    24541 tcggcgagat aacacaagtt tatgatggta gtgaaggact gttcgagttg gaaagtggag
    24601 tttatgatct tgcaatcttg gatttgatga tgccggaaat gaatggatat gaagtgttag
    24661 caaaaatacg gaaagaaaaa atacagactc cagtattgat tttgactgca aaagatagtt
    24721 tggaagataa gattgaagga tttgaacgag gtgcagatga ttatctaaca aaacctttct
    24781 atcgagaaga actgatcatg cgcgtgaaag cattattgaa acgctcgcta ggtctctttg
    24841 atgaaaatgt tctgaagtat aaggatatca gttgtgattt aaaaagcaat gaagtcttgt
    24901 ataaagaaca agttttgccc atacaaggaa aagagtttga attgctcgtt tattttttac
    24961 aaaataaggg aatcattttg acaaaagagc agatatttga ccggatctgg ggatttgatt
    25021 ctgatacgac gattacagtt attgaagttt atatgagcaa cttacgaaaa catctgcgaa
    25081 gtactggatt ggataaagaa atcaaaacct tacgtaatgt cggttatata ctgcaaaggg
    25141 tgtgatgcaa tgaatggaga tattacacga aagcaacgta gtcgcttttt tgcgacaaat
    25201 gtagcagcat ttgcttttat cttttttctt ttgggattca ttactttaca gatattaaac
    25261 caatctgcat acagagaaac agatctgtca ttagagaaaa taaattttga ttctcgaaca
    25321 gtacaattag agatacaaag atatcagcaa ggcgatccct ttttaaaccc gactcaaacg
    25381 gaacaaaaca gaacaaacat ggaacagcca ttaatggaac catcaaatga ccgttttaac
    25441 actcaagtga tcttgtggtc taaaaatggt gagatattga ataaagcagc tattggtggg
    25501 cgtttcaacc aaatcaaaga attaaaaatg gatacgaaaa atctgaatac catccaaaat
    25561 attgaattaa atgaagacag tggctcatct gccctctctt tccgttcgat tacgaaaaaa
    25621 gcggagacga atcaagatgg cgttgcctac atccaaattt tagaaaatac aaatcaaatc
    25681 aagaattcat tagcgacttt tcgcacgatt atgattttgt gtatgattgt tttttggatt
    25741 ctctctattg gtatcagtta ttacttgtct agtctaagca tgcgccctat tattctttct
    25801 tggagaagac aaaaagaatt tgttgagaat gcttctcatg aattgcgcac accgttaacg
    25861 attattcaaa atagtttgga acatcttttt accaagccta atcatactat tattgaagaa
    25921 tccgaaagca tcgctcaagc attgtcagag acgaggcgcc tcactggatt gacttcggat
    25981 ttgctgacaa tcgctcggaa tgattctgat cagcagctgc tttcaaagca aatgatcaat
    26041 acacaagaat atatcgaaca acttgttaaa ccatttcaag agatggccat catagatgga
    26101 aaagagttta ttctccataa cgatgcaaca ttagaagtac caattgatga aaaaaaaatc
    26161 catcaagttt tggttatttt gctggataat gcattaaaat atacgaatcc agaagataag
    26221 atcattttgg attcatctgt taaccataaa gaatggctga ttcacgtaaa gaatactggc
    26281 ccttctatca gtgaagaaga tcaagcaagg atttttgatc gattttatcg ggaagatcaa
    26341 tctcgggcga aagagactgg cggatacggg cttggcttgg caattgcaaa acaggtaatc
    26401 gaacagcatc atggacagat ttctgtatcc aattatcttc cacaaggagt cgattttaaa
    26461 gtccaattgc cgttgaagcc taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa
    26521 aatcttgtca cagccctttt ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg
    26581 aaataagccg aaatttcttt aagctgacca cttctgtcac aacctcttta gcttacgctt
    26641 acgatttttt attgcggtct tcaatgtgta catcaccgct tgtgtcaata tccaattctt
    26701 ctcggctaac atcttctgtc actttttcaa catcttcatg ttcttcttta tgaatcccaa
    26761 cttcttcacg aataactgta tgtttgctta catctacttg ctcttctttg attgggatag
    26821 taatcgtgtc atcttccaat ttttcgtctg ttccctgtga agattgatct gttactggtt
    26881 tgcgttcgat gacgatttct tcacgtttga ccggtacttc taccgtttca gtatcgttga
    26941 ccacatgttt gtggatgtcg acttctccag ttgtcacatc atgtgtattt acatctaatt
    27001 tttcttcttt tagttgaatt gtatcatcat ctagatttgg tttgcgatcg atgtcttcag
    27061 tcttgcttgt acccattgta tcagtctcgc caggtacgtc catcggatca gttcctggtg
    27121 tcatattcaa tggatccgtt gtattttgaa agcctgtcgt gccacctacg tccattggat
    27181 ctgttccagt gccagtttct aatccagtcc ccattgtgct tgctgggcta acagtatctt
    27241 gtgtatccat tgtatcttct gttgtttctg gctgacgata gccttttact gtgatgacaa
    27301 attttccatc agaaatatct cctctatatt gatataaagg atcattttct ggtgtagccg
    27361 tctcagaatc ataggtatca aaagaaaaag tatcttttat tttttcccat aaagaacggt
    27421 cttcttcacc atccatccga ttttcatctg tttcaacatc tataccagac aatccgtcaa
    27481 atcctagtct agatgctgct tcatcagtag tataaataac gatatcatct cgttcatacc
    27541 cttcgttacg caaccgttca attactgata atgcttcttc cctcgtacta tagcttccga
    27601 tgaccgttaa atttttctct tccatgtaaa ttcctcctag caaatagatt tatgattggt
    27661 atattgttac ttataaattt attctatagg agacgattgt tttctacaaa tattt
//
LOCUS       DASJSZ010000032        27632 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.032, whole genome shotgun sequence.
ACCESSION   DASJSZ010000032 DASJSZ010000000
VERSION     DASJSZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.032"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(287..649)
                     /locus_tag="U4B03_001280"
     CDS             complement(287..649)
                     /locus_tag="U4B03_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068142.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="U4B03_001281"
     CDS             complement(723..1103)
                     /locus_tag="U4B03_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEN2068143.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="U4B03_001282"
     CDS             1167..2360
                     /locus_tag="U4B03_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2068144.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="U4B03_001283"
     CDS             complement(2437..3021)
                     /locus_tag="U4B03_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2068145.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="U4B03_001284"
     CDS             3222..4220
                     /locus_tag="U4B03_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2068146.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="U4B03_001285"
     CDS             4332..4841
                     /locus_tag="U4B03_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEN2068147.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="U4B03_001286"
     CDS             complement(4871..5530)
                     /locus_tag="U4B03_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEN2068148.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="U4B03_001287"
     CDS             complement(5532..6296)
                     /locus_tag="U4B03_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEN2068149.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="U4B03_001288"
     CDS             complement(6304..6981)
                     /locus_tag="U4B03_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEN2068150.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="U4B03_001289"
     CDS             complement(7018..8403)
                     /locus_tag="U4B03_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2068151.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="U4B03_001290"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U4B03_001290"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="U4B03_001291"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="U4B03_001291"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEN2068152.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9341..10198
                     /locus_tag="U4B03_001292"
     CDS             9341..10198
                     /locus_tag="U4B03_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2068153.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10279..10614)
                     /locus_tag="U4B03_001293"
     CDS             complement(10279..10614)
                     /locus_tag="U4B03_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068154.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10681..12051)
                     /locus_tag="U4B03_001294"
     CDS             complement(10681..12051)
                     /locus_tag="U4B03_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEN2068155.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12129..12665)
                     /locus_tag="U4B03_001295"
     CDS             complement(12129..12665)
                     /locus_tag="U4B03_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2068156.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12974..14455
                     /locus_tag="U4B03_001296"
     CDS             12974..14455
                     /locus_tag="U4B03_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2068157.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14558..15265)
                     /locus_tag="U4B03_001297"
     CDS             complement(14558..15265)
                     /locus_tag="U4B03_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEN2068158.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15344..15682)
                     /locus_tag="U4B03_001298"
     CDS             complement(15344..15682)
                     /locus_tag="U4B03_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEN2068159.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15793..16755)
                     /locus_tag="U4B03_001299"
     CDS             complement(15793..16755)
                     /locus_tag="U4B03_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEN2068160.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16770..18101)
                     /locus_tag="U4B03_001300"
     CDS             complement(16770..18101)
                     /locus_tag="U4B03_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2068161.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18109..18789)
                     /locus_tag="U4B03_001301"
     CDS             complement(18109..18789)
                     /locus_tag="U4B03_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEN2068162.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19008..19487)
                     /locus_tag="U4B03_001302"
     CDS             complement(19008..19487)
                     /locus_tag="U4B03_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEN2068163.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19614..21293)
                     /locus_tag="U4B03_001303"
     CDS             complement(19614..21293)
                     /locus_tag="U4B03_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN2068164.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21841..23343
                     /locus_tag="U4B03_001304"
     CDS             21841..23343
                     /locus_tag="U4B03_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068165.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23356..24018
                     /locus_tag="U4B03_001305"
     CDS             23356..24018
                     /locus_tag="U4B03_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068166.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24119..24322)
                     /locus_tag="U4B03_001306"
     CDS             complement(24119..24322)
                     /locus_tag="U4B03_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN2068167.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24433..25617)
                     /gene="iadA"
                     /locus_tag="U4B03_001307"
     CDS             complement(24433..25617)
                     /gene="iadA"
                     /locus_tag="U4B03_001307"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEN2068168.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25774..27513)
                     /gene="yfcC"
                     /locus_tag="U4B03_001308"
     CDS             complement(25774..27513)
                     /gene="yfcC"
                     /locus_tag="U4B03_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN2068169.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8230 a         5768 c         4673 g         8961 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga caactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 ctttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact
     9361 tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc
     9421 ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac
     9481 cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatca
     9541 ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca
     9601 cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt
     9661 tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat
     9721 aaaccgtgat tatcttgatg ctactgaaat caacggtatt tttctatctt tagatgctga
     9781 ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt
     9841 ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca
     9901 cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa
     9961 agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat
    10021 cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct
    10081 tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct
    10141 aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag
    10201 atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg
    10261 ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac
    10321 atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt
    10381 gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat
    10441 ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg
    10501 agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac
    10561 actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat
    10621 cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac
    10681 tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc
    10741 aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg
    10801 aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat
    10861 attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt
    10921 gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa
    10981 ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg
    11041 aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact
    11101 tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc
    11161 aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc
    11221 agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc
    11281 aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc
    11341 aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga
    11401 caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat
    11461 tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg
    11521 atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc
    11581 gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat
    11641 ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc
    11701 accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc
    11761 aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt
    11821 tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct
    11881 atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt
    11941 tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag
    12001 acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt
    12061 tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat
    12121 gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg
    12181 tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc
    12241 aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg
    12301 tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat
    12361 tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca
    12421 atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt
    12481 tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat
    12541 gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct
    12601 tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc
    12661 atcattctta cctccgtctc ttttcatcta cccataaata aataacataa ataattctaa
    12721 aaatattatt gataattaac aaaacatatt tagttatatc cattaaaaat caatttttcc
    12781 gccttaaaaa caagaatatc ttgacaaaat accctaggaa ataaacaata gaataaacaa
    12841 ggtaacaaaa aaattaaata acgttatata aaaaagaagg cgacactctg tgagaataac
    12901 acagactgaa atgactttcc actcacagtt tttgtctaac tatatacaaa aacagaataa
    12961 aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag ttacttgttc tacaaatggt
    13021 cattcaaaaa aataattgtt ctttacaaga attatccaat gacttacata ttccaaaacg
    13081 gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc aagcaatttt tattgattga
    13141 tgaatttatt tattctaatc ataaaggaga gatccaaacc aaagaaaccg atcaaactaa
    13201 aaagttagct gttttttcta agttaaaatt atgctaccta aaagaatcca atcgttttaa
    13261 ctatttgatg ttactaatca attatccaca aactgctgta ccaaaaaaat atttacttga
    13321 acaactttat atatcgccct cctatctcga aaaactgaca aaacagctca atcattcctt
    13381 aataaaattc catttacgga tcctatctgt aaaaaattgc tgtactcttg aaggaaatga
    13441 gttatctatc cgtctctatc tttacttttt tctttcagat acatttggag gtaacgattg
    13501 gccattttcc tcacttgaat taaatgcact aaaaatgaac agtacagatg agcaaataga
    13561 aaaaatttca aaacaagaaa aaaatatcta cttgctgtta actttattca tattacgaaa
    13621 caaacaacag gcatattttc caaaaacttc ttctgaaatc actgaattga tggacttatt
    13681 gcagcataca catgattatt cagaatactt tacttctttt tcagtgagtc gcccttgtga
    13741 agagacagaa acagagagag cctactttaa ttattttttc tattgttttt ttccaaatca
    13801 aatctctttt cgacaaaaac aggagattgg ttttttattt tccacaagca aaaaccttat
    13861 ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa cagtttcctc tcaaccattt
    13921 agaaaataaa ataccaattt atttttattt tcttgtgttg gcagttgttt ttttcgaact
    13981 gttgggtgaa tccagcagta gtttttctac actttgcttt ccagaagcca cgtccaatca
    14041 ttctagttct tttatacagg aaaagaaatt aagagaaaaa atcgatacaa ttttttcaaa
    14101 aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa ttgatccaac acttactaca
    14161 aacagaaacg aaagagcagt tactcatcca tttgcagata accaaacagt ttacaggaaa
    14221 ttatcatctt caaagccatt tgcagcagtt atttcgcaaa gatgctgtta aaattaccgc
    14281 atattcagaa catgctgatt tgatcattac agatcagcat gagaaaattt cagatgaaac
    14341 ggagactttc tatctggata caacgcacaa tacagtgtta tttggcaagt taataaaagc
    14401 ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa acaaagagga tgtgacaaaa
    14461 ttcttgtcac atcctcttat tccaaataaa tggtattcaa tcagctaact ctcggtatta
    14521 gctcagatca aagcgctgat ttcacaactt ctttatttta atctcttcgg ttgccgccga
    14581 acaataagat caagcgcaac agctgcagga atgtcgaaag cgctgctgca acataagtca
    14641 gtgctgctgc aaataatacg tgtcttgcca tcggtacttc ttcttccgtc agtagtccgc
    14701 cttcactcaa aatttggatt gctcgccgag acgcgttaaa ttcgactggt aatgtcacca
    14761 attggaaaag taatgctaaa gaaaaagcga aaatcccgat attgattaac gtagagttcc
    14821 agctaagcaa tacacctacc ataatcaagg ggaacgaaat catcgaacca aagttagctg
    14881 ctggaacaag tgctgcacga atcttcaatg ggacataacc cgaagcatct tgtaccgcat
    14941 gtccgcattc atgagctgct acacctatcg cagcaactga agtagactgt gcagttgctt
    15001 gagacaaact caacatctta ttacctgaat tgtagttatc tgtgagatct ccggcaattt
    15061 gttgaacacc gacattattt atctgttgac tttgtaatat atattgtgct gcttgtgtac
    15121 cagtcacatt gtttttgctt ctcacttgat cgtattttcg gaaagtacta ttgacgtacg
    15181 ctgacgcaat tccggaaata actaatccaa gaatgaccaa aagaaatgtc ggatctaaaa
    15241 aaccatacat tccataacca tacattttcg tttcactcct tttcgtttac tttattttat
    15301 cattagtagt aggaaaagaa tgtgaaaaga ccttgaatat tctttaattc tttttcacaa
    15361 attccgattt cagtttcatc ggtccaaatc cttctacctt gcaatcgata ttatgatcgc
    15421 cttctactaa acggatgttc ttgacttttg ttccttgttt aatggcacct gacgcacctt
    15481 ttactttcaa gtctttaatc actgtcacgc tatcgccctc taacagacgg tttccatttg
    15541 catctaatac cactaatccg tcttctatga cagtttcttg ttcttctttg ttccattcgt
    15601 tcccgcattc tgggcaaatc aacaagcctc gatcttcata tgtgtactct gagccacatt
    15661 ctgggcaatt tggtaatgtc atcttctttc tcctttaaaa tctttagtga gcactattgt
    15721 aacggaaaaa gaagatcttg aaaagataca tttgtgattt ttactacaaa aggcacgttt
    15781 tcgaacgaag tatcattctg tttttctgct cttatacgat tggcatatat cttggatctc
    15841 tttatagatt tcctctactt ctgctaaatc ttttgcattc gcaccacttg ctaatggatg
    15901 gcctcctcca tgatggcgtt tagccaattc attgatgatc ggccccttag aacgtaaacg
    15961 tacgcggtaa taccctgaag gctgctgaac aaaaatcccc caagccagca cttcgtcaat
    16021 cactccagga agtgaaacga tcgaagctgt ctctgaatca tcgattccat atttatcaag
    16081 aatttcttgc ggaaggatta ttcttcctgc accattttca tccacctgga tattttgata
    16141 aagataaccg gaaagtttag cgactttcat cggcatttgt tcaatctctc tgttcaaagc
    16201 acttgcatca aatggataat ttctcaatat cgaagcaatc tctaatgtac gggacgtcgt
    16261 ggagggatac aaaaagcgcc ctgtatctcc gacgattcct gcataaagta accgtgcagc
    16321 cttaacgttc atctttaaag attcaggaaa catcaaagca aattctgcaa tcatctcact
    16381 acagctactt gcttttgtat ttacccacac aagatctcca taagggtcat cattgggatg
    16441 atggtcgatt ttgatcagtt tttctgccaa ctgatatctt tgatcactga ttctgggtga
    16501 attagctgta tcagtcacga tgactagcgc tccttggtag aagacatctt caacttcttg
    16561 catttctgct aaaaagcgaa gcccttcaat cgtttcacct acttgacgta tttctttttc
    16621 tgggaaactt tcccgtaata gttcggctaa tcctacttgt gatcctaatg catcaggatc
    16681 agggcgcaaa tgcctgtgga taatgatttt atcgtatttt tttattgtat ctactatttc
    16741 ttgctgtact gtcatttcac tcctgctttc taagaacgtt ccattacttg acaaacaacg
    16801 attgctttgg aaacgatggt gttttcaata aacacttcga tatccagttt agcggaacgt
    16861 ctgccgattt ccaatacacg tggacgaatg tctaattcgc tttccaattg gatcagccgc
    16921 aaataatgta ggttcacttg ttcaatcaag atatttcgtt tttggttcat caccattgtt
    16981 ttttgtgcga catcagaaat gatttcgcta agtacgccaa aagaaatggt tccaacgcca
    17041 ttgaccattt gtggtgctac tacgaatttg aacttagggt tttccaaacg atttccttca
    17101 cggtcagtat caatcgtatg gatatttcca gatatctgat ccgaaatcgt atctgcaatc
    17161 tgcggttgtc gctggacaag ctgcatcgct ttcattacat cttgccgggt aatcaagcct
    17221 tccaatgtca agtcatcgga aacgaccggc atgacttcta gcccatccca gatcatttga
    17281 tggctaacag aggcaatact cattgttttc tttacactga ttggttcttt cgtcatcacc
    17341 cgatcgatga tctggtttgg cgatttccct aaaacatctt ttgccgtaac aatccccatc
    17401 aaacgatgat ggcggttgac gactggaaaa cgcgaatgct gcgtttcttc tgacaaacgc
    17461 tgataatcag aaatcgtatc tgtctgctgc aaataatgag tcttttcagc tggcatatag
    17521 atatcactga caagcatgat atcttttttg atcaattggt cgcttaacgc tcggttgatc
    17581 atggttgcga ctgtaaatgt atcataagag gtgcgtaaga tcggcatttc aagatcatca
    17641 gctagctttg caatctcagc agtcgtattg aatcctcctg tgatcaatac tgcggcgcca
    17701 tcttccaatg ctaatttttg gacttccgcc cggttgccga cgatcattag tgatcctggc
    17761 gtaatatagc gttccatggc atgtttagtc atcgcgccga tgacgaattt attcaagacc
    17821 ttgtctaatc ctgccgcccc gcctaaaacg tccccttcaa tgatcctgac gacctcgcca
    17881 aatgtcaaac gttcaatatg tttttttaat ttgcgttcga tacggatggt ccctactcgt
    17941 tgaattgttg aaactagtcc gacattttct gcatctttga ttgcacggta cgctgttcct
    18001 tcactgacat tcaattcttt tgcgatgctt cttacagaaa ttcgattgcc aacaggtaaa
    18061 ctttcaatat attctaaaat caaatcatgt tttgtcgcca ttttctgcct acacttccag
    18121 cgtttccgag acgctcatta tttttccttc actatcttcc agtaactcta caaaagattc
    18181 gggatcttgt tcaatgacag ggaaagtatt gtaatggatt ggaactactt tcttggcacg
    18241 aattgtttgc gcagcctttg ctgcatcttc tggccccatc gtatagttat cgccaatcgg
    18301 caagaaagcg acatcaatat cgaattcttc tgcaagcaat cctagatcac tgaaataagc
    18361 agtatcccct gcatgataaa tcgtttttcc ttctgcttga ataataattc cgctaggctc
    18421 gcccatatac tgaacgatac cgtctacttc atagcttgaa ctatgctgtg catgtacgaa
    18481 ttttaccacg ccgaatggaa acgcatgttt tccaccgata ttcataccga atgaatctac
    18541 tcctctgctg ttcgcataat cagcaatctc aacaatactg ataattgtgg cttgattttc
    18601 ttttgcaata ggaatcatat ctcctatgtg gtcactatga ccatgagtaa tcagcaacca
    18661 atctgtcttt acttcttctg cagttaagtc tgtcaatgga tttcctgtaa taaacgggtc
    18721 aatcatcacg cgttggccat tttccatttc aattgttaaa caggaatgtc catgatacgt
    18781 aattttcata ttttacacct cgcgttttct ctgtatcttt tttattaaaa acgatttcat
    18841 cacttgcaca tcaatgaaaa aatatagctg ctaaatatat tttcattgga ttgatagtac
    18901 agtatgtaca aagcaattgt aacagataat acaggatgca ccaagtacca agcagatata
    18961 atctcaagtt tgaggtctct gctctgctta gcttctcttc tcgtccctta actaattaaa
    19021 tcagcgtaaa taggatggac gatcttttct aaatcggcta cttttatccg aaggcttctc
    19081 ccgatttcac cgcttgacac aattacttct tctttttctt ttaaagaact gtctgctaaa
    19141 aaacaataat caggatgtgc gagttttatt ccaatagggg tgtttgctcc gtggggatat
    19201 cctgtatgca ttaatacaaa atccattcca ggaaaaccaa ctttacgatc tttcgtcact
    19261 tgtctcgttt ttttgtaatc taagtgatcg ttcaaaggaa tcaatgcgat gatcacccca
    19321 gttttgtttc ctttcactac gagagtttta catatcaaag ctggatcaat ccctcttttt
    19381 tgtagttctt ctgtgaaaga ttcttctacc acatgactaa aatcaagtgg ttgatagctg
    19441 atcttttgtt cttctaaata acgttcgact tttgtttgac tcatcattga ttcactccct
    19501 ttattctatc gtttattata aagcgaggga tgctgtattc aaaaattatt tgtttttcgc
    19561 aaaaaaagag caagacgagc actctataga gaggttcatc ttactctttt tgtttatttt
    19621 ttatttcgac gttgagtagc ttccataatg actggcaaga tcactgggcg gcgttttgtt
    19681 tgttcaaata agtaacggcc taattgatct cggatgtctt gctttaattt gctccactca
    19741 aaatcattgc tttctaaatg tttctcaacg attgtttcaa tcatattgct gctttctttc
    19801 atcagatcac ggctagcttt tacgtaaacg aatccccgtg aagtaatttg aggttttgaa
    19861 atgattttct tttctcgccg actgatagtg acaaccgcta cgaaaatccc gtcttctgac
    19921 aagatttttc gatcacgtaa aacaatgttt ccgatatcac ctatgcctaa tccatcaatc
    19981 attacatttt ctgcacttgt agttcctgca acactcatac gcttgttctt atattccagc
    20041 acgtctccgc gtccagtaat atagatattt ttgtatggca tccctactgc atgagccaaa
    20101 tctgcatgag cagctaattc acgatactct ccttgaactg gcacaaaata tgttggtttc
    20161 atcaaattga tcattaattg caagtcatta ggattggcat ggccagaaac gcgcatattc
    20221 tcagagatca acttcactgt tccaccagct cgataaatga tatcttccgt tttcgccacg
    20281 atggtttcca tcgcgatgct tggcgtagta gtgatataaa ccaaatctcc ttcttcaatc
    20341 ttgacattac ggtgtgtacc gtttgccatt ttttgcagag acttgatggg ttcacccatt
    20401 cggccagttt ctaaaatgat cagttcttct ggatcatagt ttttcatgtc tttcaacgta
    20461 acaagcagat cttcacttgg taattgaagt ttttctaaac gcatcgctgt gcgaataatt
    20521 cgttcgaaat cttgaccagt caagacaact tttcgttgtg ctttttctgc tgcatccaat
    20581 acttgttgca ctcgttgcaa attacttgct acacaagcga cgatgatacg cccttcccaa
    20641 tatttgatcg tatcaaatac ttcatctgct atttgggctt cagaagctac aggctgagga
    20701 ttttctgcat ttgccgattc acttagtaag gctaagactc cttctttgcc gatttccgcc
    20761 aatcgtgcat aatctgtttg atacattggg atagccgttt ggtcaaattt aaaatctccc
    20821 gtataaacga tactaccttc ttttgttttc agattgatcc ccactgaatc tggtatcgta
    20881 tgagtggtac ggaaaaagct cactactgtt tcaccaaagt ctatttctgt atgctcatct
    20941 accacatgga aatccttaaa tcctttggct gcttcattac gactgacatt caattttgct
    21001 aactcgatcg tcaactctgt cccgaatact ggtacagaaa tttttgacaa caagtaagga
    21061 agtgccccga tcgcatctgc atgtccatgt gttaaaaaga caccagctac acgatcaatg
    21121 ttttcttcta aatacgtaaa atcagggatt acgacgtcga tccccaacaa ctcattttca
    21181 ggatatttta gcccacaatc taaaataaaa atatcctcgc ccacttctgc gacatacatg
    21241 ttttttccat tttcacgaac gccgcctaat gggataattt taatggtact cacaaacttc
    21301 acctcttctt atgacatcca agtttaaaga tgtcgatttt cttctttatc taaatatatg
    21361 agccattcca atgaagcata tgcaaaatca ctatttctca acttatggag aatcagcagg
    21421 aaaattacca gcacatccgc gttctgacag ttggatgggc attttatttt ggatatactt
    21481 cattaacaaa atggatcttt tgcgaaacgc tatagccaga atacttatat aagtattcag
    21541 ctgttcccca ttattataca acaaataaac taaatcgcta gtatcagcaa ttgttagaac
    21601 aactatgcac gtaaaaatcc ctctgtagca tctaggattt cttttgctat tcctctttca
    21661 tttcttcttc gtcggtttga aacatacttt tttcatttta ccacattttt tgcaataact
    21721 atagtgactt tttatatata cagaacctca tgttgatggt gtgcaaacag cataagattt
    21781 aagctagctt taagataggt tgtctattat caaatgcatc tactaggagg gatgcaaaat
    21841 atgaatttta tgaaacgtgc atggaaaagt acaaaagtaa aatggggacg ttcattgtta
    21901 ttgtttgccg tgttcacagc cattcttatt tttgtgctag ctggcctcac gattcgaagc
    21961 gctgcagaag aagcagcgaa tcaagcacaa aaagaagttg gagcaacagt gactttaagt
    22021 gccaatcgag aagcttcatt taaaaaacag gaacaaactt cttcaagcgg ttctaccact
    22081 gaagaacgtc cagatccagg cagtttttct cttacaccag tttccttaac ggacgcggaa
    22141 aaaatagctg aattagataa tgtaaagagt tattcctttg aaagttccgc ctcagctatg
    22201 gcaaaagatg gaataactgc catcactagt tctgattcaa gtgaagacag ttcagaaact
    22261 actgatgcag gcggtgaacc tgctggagga atgggcggga atccgcaaat gactcaagca
    22321 gactttcaaa tcagtggtgt gtcagcaact gcacaaaata gtaatttttc tgatggaaca
    22381 gcaaaaatca cggatggtga aggaatagat gacagcgaca aaggcacaaa caatgtagtc
    22441 attgattcta ctctagcaga aacaaatgac ctggcagtcg gagatacttt cacaatcacg
    22501 agtactgaag atgaagatac cacttatgaa atgacgatca aaggaattta tgaatccagc
    22561 gaaacaagcg gcagtatggg tatgaaattc aacttcatga atccgtcgaa tacactttac
    22621 acttcttaca cttttgcaaa tgaactaaat gggacatctg acgataacac gatagattct
    22681 gctatttaca ctctatctga tccagataaa atggatgagt ttctcaaaga agctgagaag
    22741 ctgatcgaca cagatacatt tagcttacaa tcaaatgatt ccatgtatca gtcaatgttg
    22801 gaacctttaa acaacgtggc aagtttttct aaaaatgttg ttttactagt ggctgtagcc
    22861 ggtattatca tacttactct gatcattatg attaccattc gggagcgtcg gcatgagtta
    22921 ggcgtcttgc tatctttagg agaatccaga agtaaaatca ttctccaatt gtttaccgaa
    22981 gtggctattt gtatgattct tgcattagga gttgcttcgc ttagcggaaa cgtcgtcgcg
    23041 aatgccgtgg gacagcagct acttgaccaa cagacagaaa ccacgacaca aaaccagcaa
    23101 gctcctggcg gtggacaaat gccaggtaac agacaagaac aaggcggtca acgcgatggt
    23161 caacaaggaa ccacgaataa tccatttgct gtttctgatc aagtcagcga attggaaatc
    23221 aatgtcaaac ctgcacaatt gggactatta gccggagtag catttggaat cagtctgttt
    23281 tctgtctttt tagcttctat agggatatta agattaaacc caagaaaaat cctattaaac
    23341 taaaggagga atttgatgtt acggacagaa caattgggat tttggtatca agatccgcaa
    23401 gatgcgcttt ataaagatgt agaactgaat ttcgaagctg gaaaaatgta cgctatttta
    23461 ggagcaagtg gttctggaaa aacgactttt ctttcgctta tttctggact agatgcacca
    23521 aaagaaggaa aggtatacta tcaagatcgt tcactgaaca aaatcgggtt aagaagttat
    23581 cgtaaaaatg atgtctcgat tattttccaa gcttacaact tgcttcctta tttatcggct
    23641 ctagacaacg tcatagcagc aatggaaatc actaagacca agcaagagaa ttatcgacaa
    23701 gttgctttag atcgactggc tcgtgtagga attgatgaaa tattagcaaa aaaacaggtg
    23761 accaaacttt cgggaggtca gcagcagcga gtcgctatcg ttcgtgctat ctgctgtgaa
    23821 catcaactaa tcgtagcgga tgagcctact ggaaatctag atgaaacaac ttcacaagat
    23881 atcgtcaaac ttttccaaga aatcgcccat gaacaaaatc gctgtattat catcgtgact
    23941 catgagattg aagtagcaaa aagctgtgat gaagtctttg aactaaaaaa taaaaaattt
    24001 caaaaaatcg aagtttaaaa aacaggataa tgatcaaata aaaaatccga accatatctg
    24061 tctcgcagat atggttcgga ttttttattt gcttgattca attagtaata agtggttttt
    24121 attttttttc gtcttttcga gaaaacttgt ctttgacttc gtcgcctaaa tttcgagctt
    24181 tttctttgat gtcagcacct agtgattgtg ctttcccttc tgtttctttg ctcttgtcgt
    24241 ctgttgcttt gccgtatgct tcttttcctt caccgactac tttatctttc atatctttag
    24301 aacgtccttt taaatctgcc attgtgcttc ctcctcttag tttgtttatt gatctacctt
    24361 cagggtaaca tcaacaacag atacagacaa acagaacgct cgttttctaa aaaagatagt
    24421 tagagctgtt gattaaaaac ttttttcgaa agtcccccat actttgactt ctttttcttg
    24481 gaccattatt tctcctttag cgataactgt atcaatatca agcgtttctt tatccaagat
    24541 acacagatct gcatcagcac caatttcgat ttttcctttt ttatccaatt tcaatatacg
    24601 tgctggatta ctggttatag ctcgtaaggc gatttccaaa gggatacttt ctttttgcac
    24661 agcttctttg atccctacta gcaatgcctt ggcacttcct actcctaacc ctaggaaatg
    24721 attgttttca tcaaaataag gcaagcttcc ttgtccatct gaggtcatcg tgaaattatc
    24781 taaagaaaca tcttcctcga tcaatcgttt cagtgctttg ctgaagcgaa cttcaccatc
    24841 tgtcttctcc caaaaatcag gatcttcgct agcagtgaag tcaatcgtgc caccacgttt
    24901 ggcaaatgcc acgcaagctt caaataattc tggtgtgcga tttgcatgag taggcaaaaa
    24961 ttgtgtgatc ggaatttctg tttcttctac gattcgtgtc aatagctcta gctttctttt
    25021 tcctccgcct aagtggacat tgatgatccc agcttttccg gataacatgc ctcctacacg
    25081 agcatctgct actgcacgag cgaactcttc aaaacttggc tgagaagaac ggtgatcgga
    25141 tactgcgatt tctccgattc cgataatttt atcaattgtc aaaaagtctt tgatgataga
    25201 atctgttact gtttttaccg gcaatcggta cgaaccttcg tatacataag tggagattcc
    25261 ttctgcttcc agacctttcg ctttgctgat caacgcagtc atatctctac cttctccgtc
    25321 tgttcccaaa caaccgacaa ctgtcgttac accagctgta gtcaattggc tcaatgtgac
    25381 ttctggcgtt cgattttgat aaccgccttc tcctcctcca cctaaaatat ggaaatgaca
    25441 gtcaatgaaa cccggggtta gtacttttcc ctcacctgaa agttcttcta cttccactcc
    25501 ttcgtagccg ccttctagct gttcttctac agccaaaatc ttcccaccgg caatcagtac
    25561 atccattttt cctaagtaat ctggtgcaaa cacttcaatt tgtcgaatga ttttcataca
    25621 tttttcctct ttctttaagt ataactgatg ataggatctt aaaaaataat ccaaacgata
    25681 ggatctttac tcctttgatt ttaccgtaaa atgacggttc ttggagtcct tatctactgt
    25741 ttggattact tttttattta caggtctcca gtactactgc agacctattg caatggcaat
    25801 catgatgaaa atagttccta aaacaaacag gaatccttgc atcttgattt ggaatcttgc
    25861 ccattttgcc cattcgatcc gtgcaacacc tagcacacca atcaagctgg ctgatacggg
    25921 tgtaaaagcg tccataaaac ctgaaccaag ctgataagca agaactgcta cttgtctgga
    25981 tacacctact aaatctgcca gtggagccat gattggcata gtcaaagctg cttgtcctga
    26041 atttgatgta acaaccaagt tgaacaaact ttggaagatg tacatcgccc aagcaccaat
    26101 gacagctgga acaccggtca acgccgtccc gatcccatgc aaaatcgtat tcaaagctga
    26161 aggaacagat gcatcagatc ctcccaagac aagaagaatc cctttggcca tacctactac
    26221 gattgctgta ccagctaaat cagctgcacc agattggaaa gaagaagcga tgtcattgac
    26281 tttcattcca tctaatttga aaagaacagc aatgattcca gcaactaatc ccatcacaaa
    26341 gaattgagaa gcgatttctg gaatgtaata gcctttttga gtcacgcccc aaacgatcca
    26401 gacaaggaca actaacatct ctaataagat caatttatgc ccaagcatga attttttatt
    26461 ttcatcagat gtctttttga tatgagaacg gaaataatca tctgatttgt aagtcaacga
    26521 actttctggt tttttacgga ttttttctgc atatatcatc atataaccag cggctaatgc
    26581 agtaacaact acccacataa tcaatcggaa tgtcgcacca gacaacacgg ggattccagc
    26641 aatcccttga gccaccgcta cgctaaacgg gctcatccaa gaggtcgcat ttccgacttg
    26701 agatgcgaca taagtcacgg ttactgctac gatcgagtca tatcctaacg caatgacaaa
    26761 tggaaccatc accattgaga atggaattac ctcttctgcc atcccgaatg tagcgccacc
    26821 gaatgaaaaa gcaaagaata atagcggtaa agctaaacgc tccagtccct tagttttact
    26881 gataaacgca tagatacctg cgtcgatcgc tccggtacgc ataataatac caaaagcacc
    26941 tccaacaaca aggatcaatg cagcgatccc tacagcagat ccatacttat ctccagatac
    27001 tagtccttca aaaacaaaat ttaagaaacc aaagccgcca aaatcatctg ttccccatat
    27061 tttggctgtt ttatgcaatt ttttagaagt atcatagata ttttcgccgt actgatcgta
    27121 aagcacatca tctgtcaagg agatttcatc tagtttttcc tgcgtaagat ttttttctcc
    27181 gtcagcaatg actttttcca atcctttttc cggaacatcc agtttttcac gttctgctgg
    27241 atgatcttgc agttcttcta attgatcaaa cacatacgac ttatccagct cgtaagcata
    27301 acggaaggaa tcttgacgta aaacagtacg agtacttgtt tcgccatttg catctttgta
    27361 ttcaatatct tgcgtgctga actttcctgc tggaacgaca aaagtcagta tccatgcaaa
    27421 gatgactaca ccaaaaataa tgacatacgt atggggtgtc gataatttag tgaagtcgcg
    27481 tttcggtttt cctgaagaat ttgttttctt caaatttatt tcttcctttc ttttttcagt
    27541 aattttgatg aataaaaaat ggataatcaa atgaattata cagtaaaaat gacatttcca
    27601 catgaataat ttattagaaa atcacttttt tc
//
LOCUS       DASJSZ010000033        27535 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.033, whole genome shotgun sequence.
ACCESSION   DASJSZ010000033 DASJSZ010000000
VERSION     DASJSZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.033"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            103..1107
                     /locus_tag="U4B03_001309"
     CDS             103..1107
                     /locus_tag="U4B03_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068170.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1108..2052
                     /locus_tag="U4B03_001310"
     CDS             1108..2052
                     /locus_tag="U4B03_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068171.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2052..3014
                     /locus_tag="U4B03_001311"
     CDS             2052..3014
                     /locus_tag="U4B03_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068172.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3041..3955
                     /locus_tag="U4B03_001312"
     CDS             3041..3955
                     /locus_tag="U4B03_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068173.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3981..5762
                     /locus_tag="U4B03_001313"
     CDS             3981..5762
                     /locus_tag="U4B03_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2068174.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6110..6796
                     /locus_tag="U4B03_001314"
     CDS             6110..6796
                     /locus_tag="U4B03_001314"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEN2068175.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6816..10397
                     /gene="smc"
                     /locus_tag="U4B03_001315"
     CDS             6816..10397
                     /gene="smc"
                     /locus_tag="U4B03_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEN2068176.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10406..11215
                     /locus_tag="U4B03_001316"
     CDS             10406..11215
                     /locus_tag="U4B03_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2068177.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11226..12224
                     /gene="ftsY"
                     /locus_tag="U4B03_001317"
     CDS             11226..12224
                     /gene="ftsY"
                     /locus_tag="U4B03_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEN2068178.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12411..14678
                     /gene="gshAB"
                     /locus_tag="U4B03_001318"
     CDS             12411..14678
                     /gene="gshAB"
                     /locus_tag="U4B03_001318"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEN2068179.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14767..15219)
                     /locus_tag="U4B03_001319"
     CDS             complement(14767..15219)
                     /locus_tag="U4B03_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEN2068180.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15369..16319
                     /locus_tag="U4B03_001320"
     CDS             15369..16319
                     /locus_tag="U4B03_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN2068181.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16312..17271
                     /locus_tag="U4B03_001321"
     CDS             16312..17271
                     /locus_tag="U4B03_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN2068182.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17268..18023
                     /locus_tag="U4B03_001322"
     CDS             17268..18023
                     /locus_tag="U4B03_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2068183.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18046..18999
                     /locus_tag="U4B03_001323"
     CDS             18046..18999
                     /locus_tag="U4B03_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2068184.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19643..19939)
                     /locus_tag="U4B03_001324"
     CDS             complement(19643..19939)
                     /locus_tag="U4B03_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEN2068185.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20294..20650
                     /locus_tag="U4B03_001325"
     CDS             20294..20650
                     /locus_tag="U4B03_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2068186.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20660..21559
                     /locus_tag="U4B03_001326"
     CDS             20660..21559
                     /locus_tag="U4B03_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEN2068187.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22076..22807
                     /locus_tag="U4B03_001327"
     CDS             22076..22807
                     /locus_tag="U4B03_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2068188.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22952..24979
                     /locus_tag="U4B03_001328"
     CDS             22952..24979
                     /locus_tag="U4B03_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN2068189.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25087..26184
                     /locus_tag="U4B03_001329"
     CDS             25087..26184
                     /locus_tag="U4B03_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN2068190.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26491..26949
                     /locus_tag="U4B03_001330"
     CDS             26491..26949
                     /locus_tag="U4B03_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2068191.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTRV"
     gene            27047..>27535
                     /locus_tag="U4B03_001331"
     CDS             27047..>27535
                     /locus_tag="U4B03_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HEN2068192.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT         9260 a         4770 c         5786 g         7719 t
ORIGIN      
        1 cacaaagata agaataacct ttgttataaa acgctttatt ctgtgtaatg tcctttttta
       61 ttaagaaaaa cttccattta ggaagcagag aggggtattc gattgtccga taaccaatta
      121 ttagaagttc agaacctaca tacaggattc cgactcaaag atgactatta cgatgcagta
      181 gatgacgttt catttacttt agacaaaaac gagattttag caatcgttgg ggaatctggg
      241 tgcgggaaaa gtaccttggc tactacgatt atgggattac ttgatcctaa taacacaaaa
      301 attacaggtg agattttata taacgatctg aatttaatcg acctgaacga aacgctctat
      361 aataaaatcc gcggaaatga tatcggaatg attttccaag atccgttgtc tgcattaaat
      421 cctctcatga gaatcgaaga ccagatcaaa gaaggacttt cctatcacac aaagatgaat
      481 gcagaacagc gtcaagctcg tgcactagaa ttgctagaac aagtagggat tcctaatcct
      541 gcgagagttg gacgccaata tccccatgaa ctatcaggcg gtatgagaca acgtgtcatc
      601 atcgcaatcg caatcgcctg caaacctcca atcgtcattg cagatgaacc gacgacagcc
      661 ctagatgtaa ccatccaagc acagattctt gatttactga aagaccttca ggaagaaaca
      721 aaaacaggga tcatcttgat cactcatgac ttaggagtcg tagcagaaat ggctgatcga
      781 gtggcagtga tgtatggcgg acagttcgtt gaagtagcag gtgtaaaaga actgtttgat
      841 catcctaagc atccttatac acgctcttta ctgaactcta taccacaaga aggaaatcat
      901 gatgcagaac tccatgtgat cgaaggtgtc gttccttctc tgaaaaacat gcctcgtaaa
      961 ggttgtcggt ttgcaccacg tattccatgg ataccagcaa gcgatcatga agaagagcct
     1021 atccttcatg aagtcgcacc gaaccacttg gtacgttgct cctgctataa gcatttccac
     1081 tttagagacg aaaaaggaga ggtgtagatg acagaattaa tcacaatcaa agacctgaaa
     1141 gttcattacc ctattcgcag tggattcttc aatcgagtaa cggatcatgt atatgctgta
     1201 gatggtgtgg attttattat cgaaaaagga aagacatatg gtttagtggg agagtctggc
     1261 tctggaaaat ctactacggg aaaagccgtt gtgggattag aaaaggtaac agccggacaa
     1321 atcatgtatg aaggaaaaga tgtaacgaag cgaagcaacc gcaaaaaaat gggttataac
     1381 aaggatgtgc agatgatctt ccaagattca atgtccagtt tgaatcctaa aaagcgagtg
     1441 ttggatatca ttgctgaacc catccgaaat tttgaacggt taagcgatca agaagaaaag
     1501 aaaaaggtaa aaggactgct agatatcgtt gggatgccgg aagatgcgct atataaatat
     1561 cctcatgaat tttcaggcgg acaacgtcaa cgtctaggag tagcccgtgc tgttgctacc
     1621 aatccaaaac tgatcgtagc cgatgaacct gtatctgctc tggatctatc tgttcaagcg
     1681 caagtattga atttcatgaa acgaatccag caagaatacg gcctcagcta tttattcatt
     1741 tcacatgatt taggtgtagt aaaacacatg tgcgacaata tcgcaattat gtacaaaggt
     1801 cgatttgtag aaatcggtac aagagaagac atttataatg acccgcgaca tatctatacg
     1861 aaacgtctgc tttcagctat tccgcaaatc gatgtagaaa atagggaaag ccacaaagaa
     1921 aatcgacgaa gagtagaaca agagtatatc caaaaccaaa aagaatacta tgatgcgact
     1981 ggtcgagtct acgatttgcg tacgatcaca actacacaca aagtggcgtt gaaagatgga
     2041 ggtgcaagct aatgtggaaa acgattctgc gacgtgtttt attgatgatc ccgcaagtta
     2101 ttattttaag tgttctgatc ttcttattag cccaagcgat gccgggggat ccatttaccg
     2161 gtttgatcaa tccaaatcag gatccggcag tgatcgaaca gatgcgacaa gcagctggct
     2221 tgaatgaccc atggtatgtc cagtacttcc gttggatcgg aaatgctttg caaggcgatt
     2281 ttggtcaaag tttcttatac aaacgctctg ttgctgactt gatcggcgaa cggatcatca
     2341 acactttata tctatcgatc ttgacagttg tccttactta tttgattgct gtgcctctgg
     2401 ggatgcttgc tggccggtat caaaattcaa tcttggataa gattgtcgta atctacaact
     2461 tctttagttt tgctgttcct ttatttatct tcggcttgat catgctattt gtttttggtt
     2521 atcgtttagg ctggttccca acaagtggat cacaaacact aggtttgcaa ggtggattta
     2581 tcactcaatg gatagaccgt ttgaaatata ttctgttacc atcaattacc caagcatttt
     2641 tggcgactgc cgtcacaatc caatatctgc gaagtgaagt aatcgattcg aaatcgctcg
     2701 attttgtcag aacagcccgt tccaaagggg taccgacgaa taaagtattc agtcgacata
     2761 ttttccgaaa tgcagcgttg ccgatggctt ctcaaatggg gtatgaaatc acttcgttga
     2821 ttgctggttc tgtagttatc gaaaagattt ttggttatcc gggagtcgga aaactattta
     2881 ttgattccat cggccaacgg gactataccg tgatcacatc cttagtattg attttaggaa
     2941 tcgcgacgct agtggggaca ttactttcag atatcatcat gagtatcgtt gacccgcgta
     3001 ttcggattca ataaaaccaa tcagaaagga gaatggatcg atgagtgaaa aaaacaatac
     3061 agcagttcaa gaaagtattc ctcctatggg gatacgaatg attgctcgag aatttaaaaa
     3121 agataaaatt gctatttttt cattaggttt gctagtggtt ttattattag tgatctttat
     3181 cggtgcgatg ctgaccgatc aagacaaagt gatgtatgtt agtatcttcg acaaatacgc
     3241 tgcaccagga acagtttcga cacaaggaca aaagttcatt ctgggagcag atgaaggcgg
     3301 acgtgatgta ttaggacaat tgatcatagg tgctagaaac tctgttacga tcggatttgc
     3361 gatcacgatc cttacttcga tcatcggggt tggattaggg atcatctctg gctactacgg
     3421 agggatcatt gacaacatct tgatgcggat cgttgatttt atcatgattc taccgatcat
     3481 gctgatcatt atcgtcttcg tatcgattat ttccagatac aatgtctggt cctttatatt
     3541 catcatgagt gcattttatt gggtagctaa agcccgatta ttccgaagca agacattatc
     3601 tgaagcaaga agagattatg tcagtgcctc aaaaacaatg ggaaccaatg attttaaaat
     3661 catgttccgc gaaatcatgc cgaatctaag ttcattaatc attactaatt tgacgatgaa
     3721 ctttgcggca aacatcggta tcgagacaac tttgactttc ttaggtttcg gcttgccggc
     3781 aaatgtacct agtttaggta cattgatcgg ttatgcaagt aacggagaag tcttagtgaa
     3841 tcggcaatgg atctggttac ccgcatcaat ccttatttta gttttgatgt tgagtatcaa
     3901 ttatgttgga caagcattca agcgttctgc agatgcgcgc caacggttag gctagtatag
     3961 aagcgaaggg ggaaatagga atgaagaaaa cgatttttgg atttgcagcg ctgatgtctg
     4021 ctgtcgttct tgcagcatgc gggggcggag gtacagatac ctctggaaac aactctggag
     4081 gcaacggcaa taacaaagag gaattaactt ttccaattgc aacaaaaaat aatgatgatg
     4141 caatcgaaga cggcgaatta gaagctgcgg tcgcaacgga cagtcagttt aaaggattgt
     4201 tccagtggga gttttaccaa gattcatatg atgcagcttt tatggctcct tctcatgaac
     4261 cgctatttgc aaacgatgac gatttcagaa tcacagacga cggagctgca acgcttgatc
     4321 tagatgaaga tacgaaaaaa gcgacaatca aagtaaaaga caacgtcaaa tggtcagatg
     4381 gtgaagatgt aacggcggac gatgtgattt tcccttacga aatcattggg aataaagatt
     4441 acactggtat ccgttatgac gacactttcc gtaacatcgt aggtatggaa gaatatcatg
     4501 atggaagtgc cgacacaatc tccggaatca aaaaaataga cgacaaaaca gttgaaatcg
     4561 aatataaaga agtaaaccca agcatgcttc aatcgggtgg tggtatctgg tcatatgcag
     4621 caccaaaaca tacgttagaa ggtgtagcaa tcaaagatat ggaatcaagc gatcaagtac
     4681 gtaaaaatcc agtgactttt ggcccttatt acatgagtaa tatcgttgca ggagaatcag
     4741 tagaatactt gccaaacgag cactactgga atggtacgcc taaattgaaa aagatcacga
     4801 tgaaatctgt accgatggca agtgcaactg aagcattgaa ctctaaatta tatgatatga
     4861 ttttccaaat gccaacagat acttacgata cctataaaga aattgctggc tatacaaact
     4921 taggccgtga acaaacttct tatacttatc taggattcaa gttaggtaaa tggaatgcag
     4981 atacaaagag tgtggaatac aatccagatt caaaaatggc tgataaatca ttacgtcaag
     5041 caatgggcta tgcattagat aataatgcag taggtgaacg tttctatgca ggtttgagaa
     5101 gcaatgcgac ttcattgatt cctccagtat tccagtcata ccatgatgat gagatgaaag
     5161 gattcacaca agatctagac aaagcgaaaa aactattaga tgatgctggc tacaaagatg
     5221 tggatgacga tggtttaaga gaagacaaag atggaaataa actaacaatc aactttgctt
     5281 cgatgtccgg tggggaaaca gcgcaaccat tggcagatta ctatatccaa caatggaaag
     5341 aaattggttt ggatgtccaa tacacaacag gtcgtctgat cgaattcaat tctttctatg
     5401 ataaattaga aaacgatgat cctgaaattg atgtttacca agctgcttgg aatacaggaa
     5461 ctgatccaaa tccaagtgga ctatggggac caaatgctgc gttcaactac actcgttatg
     5521 aatcagaaga aaatacaaaa ttgatcaacg atatcagttc aagcaaagca tttgatgaag
     5581 agtaccaaaa agaagcatat caaaaatggc aacaatatgc atttgaagaa gcatttgcga
     5641 ttccaacgtt gttccgtaat gaagtattgc cagtcaacga ccgtgtcaaa gactgggatt
     5701 ggtcttatga tgtaccaaac ccatgggcaa atgtttctgt tacagcagat tcacgttcat
     5761 aatcactaaa aatgaccgaa aaagggcgat gtcctttttc ggttttttga tatttttctg
     5821 tttatttctc taatattcag gtcaaggcgc tgatttcatg acttctccaa taaacggtgt
     5881 tcaaagtcca gtttctcggt attaagtcaa atttatgcaa aaatatggaa agccattttc
     5941 acaaaatttt ttcttcatac tcgaaactaa acgaattttt cacaacttct cgtcaatcac
     6001 taggattatt aaagctttac ttaccttttg accgaaccat ttcaaatttg acaaaaaacc
     6061 agtataataa ggaagtatcg aaaaaatatc cataaaagga atgtggaaaa tggacaacca
     6121 gctgacaaaa gaactaaaag aaaactataa catcgttttt cacgatctaa atttgcttga
     6181 acaagctttt acccattcat cctatgtgaa tgagcatcgc aatctgcaat tatcggataa
     6241 tgagcgactt gaatttttag gagatgccgt tttagaactg atggtttctc agtatttatt
     6301 ccgtttatat cctcatttgc cagaaggaaa attgacaaaa acacgagctg cgatcgttcg
     6361 cgaagacagc ttgtcgaaat ttgccaaaga atgtcacttt gatcaatatg ttttgttggg
     6421 caagggagaa gagaattcag gaggacgtac gcgaccagca ttgctttgcg acctattcga
     6481 agctttttta ggcgcgttat atcttgacca gggattcgac acgacccatg cattcatcga
     6541 aaaagtgatt tttcctaaag tcaaagcagg tgctttttca catgagatgg atcataaaac
     6601 aaaactacaa gaagtcttgc aaaaatcagg tgatgtatca atcgaatacc gattgatcaa
     6661 tgaagaaggt ccagctcatg aacgagtatt ttggatcgaa gtttatgtag atgatcaatt
     6721 gattggcact ggacaaggga aatcgaaaaa attagcagaa caagctgcgg cggaaaatgc
     6781 attggcagca ctgtaaaagt aagtgaggga aagctttgta tttgaaacga attgaaatag
     6841 ccggttttaa atcatttgca gacaagacag tcattgattt tgaaaatagc gtcacagcag
     6901 tggttggacc aaatggcagc ggaaaaagca atattacaga ggctatccgc tgggtattag
     6961 gagaacaatc tgctaaaagt ctgcgaggcg gaaaaatgcc ggatatcatc tttgccggtt
     7021 ccgatacccg taagcagctg aatattgcag aagtcacagt gattttagac aatagtgatc
     7081 attatctgcc attggagtac aatgagatca gtgtaacccg ccgttatcga cggactgggg
     7141 aaagcgagtt tttcatcaat aaacaaagct gcagattaaa agatatccaa gaattgttct
     7201 tagattctgg attaggaaaa gaatcgttct caatcatttc tcaaggaaaa gtagaagcga
     7261 ttttcagtag taaaccggaa gaccgtcgtg ggatctttga agaagcagct ggtgtcttga
     7321 aatacaagca gcggaaaaag aaagcagaac agaagctttt tgaaacagaa gacaacctca
     7381 gccgcgtcca agacatcatc cacgaattgg aagaacagtt aactcctttg gccgcccaga
     7441 gtgaggcagc aaaggagttt ttacggctaa aagaaacact gactcaaacc gatgtctctt
     7501 tgatggttgc tgaaatcaag acagcaaaaa aagattggga caacaaacaa gcacagcttg
     7561 cgaaattcaa tctagaatta ggcaaactat ctgagtcgat ccaagaacaa gaatccattt
     7621 tggcgaagca gcgtaaagaa aatgcgcaag ccgatcgtct gatcgaaaaa aatcagcaag
     7681 tattattaga tctttcggaa aaactgaagc agacagaagg acaaaaagat gttttgcagg
     7741 agcggacaaa gcatacacaa aaaagctcgc aagaatatca aacttcatta gcagaagcac
     7801 aaaaaaaggt taaacatttt gaaaagcttc aagaaagctt gatgaaagca gcagcagaaa
     7861 aagaaacaga gatccaaaaa gctgaagcaa atctcataaa aacacagcaa gaattagaaa
     7921 aataccaaaa atcaacgaaa gaattattgg cagaattaag agatcaatat gttgatttga
     7981 tgcaagaaca agcagctgta ggaaacgaac tgaagtatct agaacgtcaa tatatacaag
     8041 aaacagcaaa aagcaagcag acgttagcca agcaatcgga agtagaagca tcagtagacc
     8101 gattgatctt acaaaaagaa gaattgacac aaaagcaggc gcagctaaaa tcatctttaa
     8161 ctgaaacaaa agagaagtta gaaatgatcc aacaaaacgg gaaaaagttc caagaagcat
     8221 tagcaaaaga acagcctaaa atgtatcaat taatgaatca agtccagcag ctacgtgctc
     8281 gtaagaaaag ccttcaagaa atccaagaaa actattttgg tttttaccaa ggggttcgat
     8341 tggtcttgca gcataaacag cagctttcgg gtattgtcgg agcagtggct gaactgatcg
     8401 atgttcctgc agatttcacg ttagctatcg aagcagcttt aggtggggca gcccagcatg
     8461 tcatcgttga aaatgaaaaa gatgctcggc aagccatcac gtatttgaaa cagcaaagag
     8521 gaggaagagc aacattcctt ccactgacga caatcaaacc aagacaatta ccggctcata
     8581 tattgacaca agcagcagct gtagaagggt ttatagggat tgccagtgaa caagtcagtt
     8641 atcccgatca aatccaaact gtggttcaaa acctgctagg aacgatcctt ttagccaaag
     8701 acttaactag tgccaatgca atcgcgcaaa cgatccgcta tcaatatcga gtggtttcct
     8761 tagaaggtga tgtgatgaat gccggaggat ccatgacagg tggagcgaat aaacgaggaa
     8821 accaaggcag tttgtttgta caaaatcaag aattgaaaca gttgactagt gaatttgaag
     8881 aagcagataa acaactccaa gcacaagaaa aaaaagttca ggagctgcaa caagaaacag
     8941 ctcgtttagc cgaagaacaa gaagttttgc gtacacgagg cgaacaatta cgttttgaag
     9001 aacaagaagc gacaaatcag ctacaaaaca tcataaatga acttgaacgt tttgaaaaag
     9061 aaaagcaaat ctcaactttt gagacacgtg aactgcagca gtttatcgaa gattacgaaa
     9121 agcaaacgaa cgaattgaaa gacaaacaaa cagatcttga atcgcaacga caaaaaatcg
     9181 acgaagaaat caaatcgtta agtcaagaaa gcgaccagat ggaagccaga agagcacaag
     9241 tacaaagtca aaaagcacaa gaacaagcag atcttgctgt cttgaaagag cagtttaatc
     9301 atctgcagat ccagctccgc ggtgcccgag tccaaaaagc agaggcaacc gaacgtcaag
     9361 aagcgataga aaagcagttg gcgacattga cagcagactt ttctgatcat gaagtcacag
     9421 aagaaagttt ggaaaaacaa attaacgaat tatctgctca gcgagaaaca ttaaaagccg
     9481 agttggcaaa agccaaagag cagcgagata gaaggcaaaa ggagatcgat aagcttgaag
     9541 cagtcttggc agagcgtaat cgtgagcaaa aagcacgact gtcggaacaa tccaaattag
     9601 aagtccaaaa agaccgagct gaaatgattt tagataatca tttgacctat ctgcagtcag
     9661 aatatcagct gacatttgag aaagcttccc aagactatca agaaacaacc gatatcgaag
     9721 acagtagaac aaaggtttca tccttaaaag aacagattga aaaactggga ccagtaaact
     9781 taaattcaat cgaacaatac gaacaagtca gtgaaagaca tacattctta gcaacacagc
     9841 gagatgatct gttagcagcg aaaaaccaat tgtttgaaac gatggatgag atggatgatg
     9901 aagtgcggac aagattcaaa gaagtatttg aagctatccg tcaagaattc aaaattgtct
     9961 ttccaaatat gtttggcggc ggtaaagctg aattagtact gacggaacca actgatttgc
    10021 tgaaaacagg tattgagatt gaagtacaac ctcctggcaa aaaactgcaa agtctaagtt
    10081 tgctttctgg aggagaacga gcacttacag ccatcgcgtt gctcttttcc attatccgtg
    10141 tctgtcctgt tccgttttgt atcttagatg aggtagaagc agcgttagat gaagccaacg
    10201 tgaaacgttt tggtcgttac ttgagcgatt tccaagatga cacacaattt atcgtagtca
    10261 cacaccgcaa aggaacgatg gtcgcagcag atgtactgta cggagtgacg atgcaggaat
    10321 ccggtgtatc aaaaatcgtt tctgtacgga tggaagatat caatgaagag gcgaaaatca
    10381 acgaaacgaa agtgtaggaa cgaaaatgat caaattagta gcaatcgatt tggacggtac
    10441 attattaaat gaacaaaagc tgatttcaga tgagaataaa caagcattag cacaagcaaa
    10501 acagcaaggg gtcaagatcg ttctatgtac aggacgcccc ttagctgcga tggcgcatta
    10561 tctgcaagaa ttaggactgg tagatgaagg tgactacagc atcactttca acggcggttt
    10621 ggtacaaaaa aatgatactg gtgaaatcat ggaaaagaaa gtgatggaag tatcagatat
    10681 ccaacgctta tacaaactgg ctcagcaatt ggatctgcca ctagatgttt tgtctgacaa
    10741 tgtggtttta cagctgccta gtgccccaag gaagcaatcc ttgtacaatg tgttgaataa
    10801 gctgctgcaa ttccaaccag cggatataag cgatattaca gatgaatttg tgcttaataa
    10861 ggcagtaatc gcttatgacc aagaagaatt agatcctaaa atcaaagaaa taccagcaga
    10921 attccatgaa atgtatgaga tcatcaaaac aagaagtatg cttttggaat ttatgccaaa
    10981 aggtgttaca aaagcttatg gtatttcttt attggcaaaa gatcttggtt tgaaacaaga
    11041 agaaatcatg gcgatcggtg atgaggaaaa tgatttaccg atgatccaat atgctggact
    11101 aggcgtagcg atggagaatg ctgtgccttt cgtcaaagaa gcagcagatt tcgtgacagc
    11161 cagcaatgaa gaaaatggcg tagccaaagt gatccaaaaa tttgtattag agtaggggga
    11221 gtcaaatggg attttttgat aaaatcaagc gtgcttttac tggcgaagag aaaacagaag
    11281 aagttgaagt acaggagaag tacgacaagg gtttagaaaa gacgcgtaaa acatttggtg
    11341 aacggatgaa tgagttgttt gccaattttc gaacagtcga tgaagacttt tttgaagaat
    11401 tagaagaaac acttatcggt gcagatgtag gttttgaaac agcgatacga atcaccgaag
    11461 cattacgtca agaagtgaaa ctcagaaatg cgaaaaaatc ggcagaagtc caaaatgcga
    11521 tcatcgagaa acttgtggat ctctatgaag cggaaggaat caatgaagta aatgaactaa
    11581 atatccaaga agacggacta acagttattt tgtttgtcgg tgttaatggt gtagggaaaa
    11641 caacaagtat tggtaaactt gcccatcagt tcaaaaacga aggtaaaaaa gtattgatgg
    11701 cagcagcaga taccttccgc gctggagcaa tcgaccagtt agttgtctgg ggcgaacgcg
    11761 ctggtgttga agttgttcgt ggcaatgcag gaggcgatcc agcagctgtc gtattcgatg
    11821 ctttggaaag agcaaagaca gagcaggcag atgttttact agttgatact gcaggccgac
    11881 tgcaaaataa agtgaacttg atgaacgaat tagaaaaaat aaaacgagta atccagcggg
    11941 aattaccaga agcaccccaa gaaacacttc ttgtattaga tgcaaccact ggtcaaaacg
    12001 cgatggttca agcaaaacaa ttcaaagaaa caacagatgt gaccggtctt gttttgacaa
    12061 aattagatgg tacagcaaag ggcggtatcg tacttgctat ccgtaatgaa ttgcatttac
    12121 cagtgaaatt ggtcggtctt ggtgaaggaa tcgatgactt ggagccgttt gatccaaatg
    12181 attttgtcgt tggattgttc aaaggattac taaaagaaga ataacccatg tctaaaaagc
    12241 aggagctgtg acaaaagttg cagctccttg tttgaatgat tgtttttaac atagctgtgt
    12301 ttttgatgcc ttcattttta tacttatttg taagcggttc tcaaacaatt tcgttctttt
    12361 tcttagactt tttgattata attagattca tatgaattgg aggtcactta atgatgaatt
    12421 ttaagcaatt attattgcat gtcaatgcgc gcccttttat cgaccaagct cgatttggta
    12481 tagaacggga aggacagcgg gttgatcttg caggaaatct agcaaaaacc gatcatccag
    12541 caatctttgg cgatcgatcc tatcacccct atatccaaac agattttagc gaaacacaaa
    12601 cagagatgat cacccctgtt accgattcta ttcccgaatt atttcagtat ctcgctgctg
    12661 tttatgatgt gactgctcgt tctataccga aagaagagat gatctggcca ttaagtatgc
    12721 cacctgcctt accggaaaaa gacgaagaga tcattattgc aaaattaaaa aatttcgaag
    12781 atgtcttgta tcgacgttat ttagcaaaaa aatacgggaa gcgaaaacag atggtaagcg
    12841 gtattcattt caattttgaa tttggcgatg agttgctaag aacattgttc agccatcagg
    12901 aagaatttca agatttttcc gaattcaaaa cagaacttta tttgaaaaca gcgagaaact
    12961 ttatgcgcta tcgctggatg atcacttatt tattcggtgc ttcaccaatg agtgagaaga
    13021 actacttttt agatgaatca catccgcaag aacctgttcg cagtattcgc aacagtgcgc
    13081 tgggctatac gaatcatcca aatgtgaaag tttcctatgc ttctatgaaa cagtatttag
    13141 cagatatcga gcgaatgatc gaagaaggga aactttcgga ggagaaagaa ttttatacac
    13201 cgcttcgttt ccgaggcgga aagaaagtcg cagatttagc aacaacaggt gttcgttata
    13261 ttgaattgcg gaatatcgat ttgaatcctt atgcaagatt gggtatcaat ccagagcaag
    13321 ttcggttctt acaattattc ctgatgtaca tgttatggac agaagaaaaa gaagactgtg
    13381 accaatgggt agcagaaggt acgactcgaa ataacaaagt agcactagaa caaccatcag
    13441 atcaaacaga atttcatcaa gaaggtagag aaattcttga aggaatgaag caaatgctgg
    13501 ttgaattgga ttggttggat tctctttatt tagtagagga agcattgact caaatggatc
    13561 atcctgaaca aacgttagca gcgaaactct atcaagaagc acagctatcg agtcagcaag
    13621 aagttgctgt ggcattggga catcaatatt ataaagaaag ccatgaacgt ccctatcagt
    13681 tggccggttt tcgcgaaatg gaactttcta ctcagatatt tatgttcgat gccatccaaa
    13741 aaggtgtcca agtcaaagta ttagatgagt cggatcaatt tttgcgtctg caattccaag
    13801 accatgtaga atatgtgaaa aatgcaaaca tgacaagtaa ggacagctat atcgtcccac
    13861 ttatcatgga aaacaaaaca gtaacgaaaa aagtcttaaa agaagctggg ttccgggtac
    13921 caggaggcgc agaattttca tccatggaag aagcggtaaa agcttatccg agatttgccg
    13981 agcaggcatt tgtgatcaaa cctaaatcaa ccaattacgg gttaggtatc acgattttca
    14041 aggaaggagc tagtctggaa gattatcaag caggtttagc cattgctttt cgtgaagaca
    14101 gttcggtttt agtagaagaa tttatgccgg gaacggaata tcgtttcttt gtgatcgacg
    14161 gagaagtcca agccatcatg ttgcgagtcc ctgctaatgt tattggtgac agtatccgaa
    14221 cagtgaaaga acttgttgaa gaaaaaaata gtgacccttt gcgtggaacg aatcatcgtg
    14281 cacctttaga attgatccaa ttaggtgaat tagaacaatt gatgttgaaa gaacaagggt
    14341 tgacgatcga atcagttccc caagccaatc aaattgttta tctaagggag aactcaaata
    14401 tcagtacagg tggtgattcg atcgatatga ctgatgaatt ttctgaagct tacaaaaaaa
    14461 tcgccgtttc tgcggtagaa gcactaggag ctaagattag tggtatcgat ttgattatac
    14521 cagataaaga aatcgatcct acaactgata aaaaagcata tgggatcatc gaagcaaatt
    14581 tcaatccagc catgcatatg catgtttatc cttttgcagg aaaaggcaga agattgacga
    14641 tgaatgtatt gaaattgtta tatcctgaag tattttaaaa gaaaaagctt ttgacagatt
    14701 gtcctatttg atgtcaatct gatcaaaagc tttttttgca tgtaaaaatt tatgtgtttt
    14761 ttcgttttaa aataaacgat cccagaagct ttttttcttt tttggttttt ctttttcagc
    14821 tggcgaagag ttttcttctg ggtatttttt ttcgatatca atcacaggtt catttactgc
    14881 ttcttttgct gttaagagca atccgaattc ctcaggattt gcagtgacgt aatcgttgac
    14941 tacagtgaaa cggctattgt ttttcgttgc tagctggata tattggtttt ggacagaaag
    15001 gggtagagcc ccattcaaga gtatggaagc atctggatgt tttttgagtt cttttaaaaa
    15061 gttttctttg ttttttggtt ggatcatttc agcaacagtc atactaagat agcagcgttc
    15121 cctgaatgtc cctaaatatt tatgctgttc ttcagggttc actaaaggtg taccataaag
    15181 tcccttatcc aaatggcttt ttacatcatc ttgtgccata aaaaataccc tccttcttct
    15241 ctttgtcttt cttacgatta tagcatattc ccaataatac ctaaaacata tgaaaaagcg
    15301 gttgcttttg ttcaacgatt cacttaaaat aagaatgaaa atcattctca attagaaagg
    15361 tggattggat gcgctacgga cgactagtag cattattagt tattttaagt agtatttcat
    15421 tgttcatcgg agtatcgaat gtacaattgt cagaactgct ccagttagat gggcaaagct
    15481 tacaaatctt gctagtgact cgtttgccac gaacgatttg tttgatcctt gtgggagcga
    15541 ccagtagtat ttgtgggttg atcatgcaac atttgaccca aaataaattt gtttcaccaa
    15601 cgactgcggg cacgatggat agtgcgcgtt taggtatttt agtggctatg attttcctac
    15661 ctggtgcatc cttgattgtc cgatccttga cagcattttg ctttgctttt gcaggaacat
    15721 tgcttttttt atcccttacg cgattgttcc tccagaaaaa tcaattgatg cttccgttga
    15781 ttggagtcat gttagggaat atcattggtt cagttgctac tttttttgct tatcaattcc
    15841 agttggtaca aaatatgact tcatggcttc aagggaattt tgcgactgtg atacgaggaa
    15901 attatgagtt attgtatgct gttgttccat tgttgctctt ggtttggcta tttgcttacc
    15961 gatttacgat cattggtctg ggcgaatcgt ttgcagtaaa tctgggaata gattaccaga
    16021 agatgcagtt tttaggcatt ggcttagtat cattggctag tgcagtcatg ttgatcatgg
    16081 taggaagcat tccttttatc ggagtgatca ttccgaatct tgtctctaga atatatggag
    16141 atcaagtgca caaaacaatt ggcgtaactg ctttagcagg tagtttgttc ttagtatttt
    16201 gcgatcttgt cgctcgcgtg ttgatttttc cttatgaaat accagtcagt gtgatcgttg
    16261 gaatcatcgg tggggctatc ttcctttatc ttctgataag gggacgacga tatgcttaaa
    16321 aaaaagccat ctatttttat tggtatgctt gttcttgttt tatttgttac aggaatgtac
    16381 ttgacgatcc atacatatgg aaactggcat tttgcatgtg cattgaggag taagaaaatt
    16441 tttgcattta tccttgtctc gctagctgtc agcagtgcca caatcagctt tcagacaatt
    16501 acgcataacc agtttttgac tccttcagta ttgggacttg agtcgttata tgtccttgtc
    16561 caaacggtcc ttttcttctt tatcggagga gtgacgatgc ttcagcagac aggtacagtt
    16621 tttttcttat tgaacgttgg gctgatggtc gggctgagtc tattgttatt tttctttctt
    16681 ttgaaaaaaa tgagagcgaa tcttttttta ttactgatgt cagggatgat tttaggtacg
    16741 ctttttggta gtgtcagtac ctttttacaa gtcatcatgg atccgaatga atatgatttg
    16801 cttcaaggca gattatttgc aagctttggc aatatccaga gtcagtatct tttgcctgct
    16861 tctttgatca ttatcggctg ctggtacctg ttatttcgat atcgacatgt gctagacgtt
    16921 ttccatctcg gaaatgaaca agcagtaaac ctcgggatag acacagatcg ttttgaattt
    16981 ttgattttat gttgtgtcag tcttttgaca gggacatcaa cggctcttgt gggtccagtt
    17041 acttttttag ggtttattgt cgcaaatatc agttatcgtg tcttttctac ttatcgtcat
    17101 ggtcagctat ttatcggaag ctttctggta gctatgctgt ttctgacagg aggacagctc
    17161 gcagtagagc agattttcgg ctggaataca acagtgagtg tcgtcgttga atttgtcgga
    17221 ggaatttatt ttgtcacaaa attgattcgc gaaaggaaga gtatcctatg attgatttac
    17281 aaaaggtcaa taaactgtac catggaaaat cagtggttaa agatgtacag ttaacgattc
    17341 ctgaaaagaa gttgactgct tttatcggtc caaatggtgc tggaaagtca acggtgttgt
    17401 cgatgatcag tcgtttgata ccaaaagata caggtgaaat ctatttagac cataacgaag
    17461 taaaggcttg gaaatccaat gaattagcca aaaaattatc gatcctacga caatcgaatg
    17521 aaataaatgt ccaaatcacc gtgaaagaat tagtagaatt tggacgattt ccttatacga
    17581 aaggaaaatt gacgacaaaa gacgaggaaa tcgttcagga atcactggaa caccttgggt
    17641 tagaagaaat agcggatcaa tcgattgaca ctctttcagg cggtcaactc caacgtgcgt
    17701 atattgcaat ggtgctagcg caagacacgg attatatttt actggatgaa cctttgaaca
    17761 atctagatat gaattttgcg gttcaaatca tgcagatttt aaaaaattta gtacaagaaa
    17821 tcgggaaaac aattgttatt gttcttcatg atatcaactt tgctgcaagt tatgcagacg
    17881 aaattgttgc aatgaaagat ggacagttat ttacgcaagg aacgacccaa gaaatgataa
    17941 aaaaagatgt attggatgaa ttatacgaaa tggacatacg gatttgtgag attgaaggcc
    18001 gcagattctg tctttatttc taaaactaac attaggagag aagagatgaa aaaattagta
    18061 gtaggtatct tgattctagc atcatttggt ttagcagcat gtggaaatag caacaacacc
    18121 agtcaggcag acacaaaaaa aagcagcaca caaactgaaa ctacattgac gataacagat
    18181 agtaatggag accaaatcga ggtgccaaat aatccagaaa aagtcgtcgt attcgacaat
    18241 ggttctttag atacgatgga tgctcttggt gttggagata aggtagttgg agcagctaca
    18301 agcagccttc ctgagtatct ttcatcctat aaaaaagtag aatcagcagg tggaataaaa
    18361 gaaccagact tagaaaaaat taatcagctt cagccagatc taatcatcat ttctggccga
    18421 caaagagatt ttcaaaaaga tctaagtcaa attgctccga ccattttcct ttctttagat
    18481 gcgaaaaatc catgggaatc tttccagcaa aatgtcactg ctttagggga gatctttggt
    18541 aagcaagaag aagctaaaac tcaattagag gaattatcta gtgcaatcga tcagacgaaa
    18601 aagaaagcag aagctactga caaaaaagca ctagtcacac ttgtgaatga agggcaatta
    18661 tcagcctacg gatctggttc tagattcggt ttcatccatg atttatttgg ctttgaacaa
    18721 gcagatgatc aaatcgaagc atccactcac ggtcaaagtg tttcttatga atatgtgctg
    18781 gaaaaaaatc cggatatctt atttgtagta gacagaacaa aagctattgg tggagacgac
    18841 agcaaagatg atatttctgc caatgaactg gtagcacaaa cgaatgctgg taaaaatcag
    18901 cagatcatct cccttgaacc agatgtctgg tatttaagcg ggggcggttt agaatcaatg
    18961 aaactgatga tcgaagatgt gaatcaagca tttaaatagc ataaataaca gaggattaaa
    19021 ctctcatact atgtaacaat aatgtttctt acattcttaa atagcaagag cctatcctca
    19081 aagaagttat caaaaaccat ccgtaaaatc gtattgatga ttttacggat ggtttttatt
    19141 tatttgagaa atcttttttc aacgtgaatt cattatctac atcatacggg ttgattctag
    19201 aatggggagt ggcaaaaaat agtgtgatcc attttttcag catcaagatc gcctcctttg
    19261 atttgaatca aaggtacaaa gcgaaactta gataaaactt aaatttagat atttttattt
    19321 tgtaccgatc tcttctttaa acgatgttcc atctgctgct tctcggcaat aagtccacta
    19381 gactagaaaa tatgagaagc tattttctgt ccagcgttct tattactcaa aacaaatcgc
    19441 agttgtcaca atctcttcta aacgatgttc catctgctgc ttctcggcaa taagcccact
    19501 agactagaaa atatgagaag ctattttctg tccagcgttc ttattactca aagcaaatcg
    19561 cagttgtcac aatctcttct aaacaatgtt caatcagctg accttcggta ttagctcaaa
    19621 gcactgattt cacaacctct atttaggcat gtgtctcagc aagcggttga tagttgatga
    19681 tctgcatttt tcgataaaaa tcggcgggaa gcttactcca ccgttttagt aattgataga
    19741 aataactctc ttctgctttg attttatact cgcttaatat caatccaaaa ctattcgttt
    19801 tttttgaagc taatttttta taaatatcca atgctttggt tttatcttca ttgacaaaga
    19861 gtgtttttac ttcaatataa gaaccctcga tattttgtgt ttcttcgata aaatagatgt
    19921 tttttatata ttccttcata gtgattcctc caaagaagtc ttttatttga taagtttact
    19981 ctaccgaaga gttgttaata atccgtgaac aaaaagtaat tctttttcca atagttgtta
    20041 aaggtttttt attttttata gtattttttg ttatttaaga aaggaataag gttaaacaaa
    20101 taacaggttg ctttttgggt ctttttccgt tcaagaaact gaacgaagaa aagggaaagt
    20161 tgtctctttt gtgataagag aaatcataat aaacgaatga cttactttcc aataaattga
    20221 gaacagtcag aaccagacaa attagttgca tctgatgaat aaagtgatta ttctatgaca
    20281 tgaggtgata cagatgaaat tagaagtgac aaaagaagca caagaagtat tgaagaataa
    20341 attagaagca gacgatcaac ttttattaga tatcgaaaac ggtgacggtc catttgcaga
    20401 aagccaagtc agttgccaat tggatacggc atttcgattg atcattgtca aaaaagatac
    20461 acaaacagat ctaagtcttt attctgtagt ggctgataca ccagttggac caatcaaaat
    20521 caaagacagt gcaatacttt acatggatga tccatccaca cttgctttag aaccaactta
    20581 taatagttta caattaaaag gaccaagcgg cttgagaaaa ggcaacttgc aagttgttcg
    20641 aaaagactag agggataaaa tgggaaaatt ggcgatcatc agcgacctac atgtggacat
    20701 caataaattc ggcgaatcag agctgatgca gctttggcaa gttttgcaaa agcacaagat
    20761 cactcggctt catctagccg gagatacggc taataaagta gaccgttgcc tagctgttgt
    20821 cgaatttttc gtcaataaag gattatctac gacatttcat ttcggcaatc acgagttggc
    20881 agatgtgccc aatgaacaga tgatggacca ttttccagac ccacattttt taaatgaacg
    20941 gtatattgct ttaaatgagc agaccgttct tttagggatg aatggctggt acgactatca
    21001 gttttccgaa ttgaaagatt acgaggcaat cgtacgaatg aagcgtttgt attggtatga
    21061 tcggttcatc cagcgtggga aaacagatct cgaggtcaat gcagaaatgc ttgcagctgc
    21121 aaaaaatttg ctagaccagc tggaagcaaa aaaacttgag gtgattttat ccactcattt
    21181 cgttcctaaa cgggcattta tcatgtatca atctgctcct tatgagcgtt ggaacaaatt
    21241 aaatgcattt ttaggatcag aatcattcgg agaattactt gaccgttatt caaatatcaa
    21301 acaagtagtt tttggccata cgcatcgtcg ctttgaagat aaatccatcg gtcacacgat
    21361 ttacagctgc agaccttttg gttattatta tgaatggcag ctgacccgag attttgtctt
    21421 gaaagaacag ttgctagaca gctatgatcc tatgaaatta cgtgtcctct tgcgtgccca
    21481 tcacccagct tttcaggcct atcaaacaca acatttgcaa gaggaatttg aacaagcgat
    21541 gacaatcgtt ccgtattgag acagcattag taatgtgtaa aagaaaatac cttgtaaaac
    21601 ataaggataa gtaagagcag tagatccatt tatcgtggat atgctgcttt ttttgcggaa
    21661 aataatcagc gaatgtttat ttgatcggat gatttgtaac tcgcagagta ctagatgcaa
    21721 gcgtgtattc tttttttgaa cgataatcac aaaaaaaaat ataatataca ttttttattt
    21781 aattataatt aatattgatt gaagtctagg cagtcaagtc acattttgat agataggagg
    21841 gggacttacg aattatttta aaggctatga cagctgtatt tctagattgt gtttgcatca
    21901 agttgcaata aaaactagaa atcaaatcgg gctgataatc atttataact aatcgataca
    21961 taggaccaaa tataggtcta tgttctttta tttcataagt atcgtaaaac agaattttgc
    22021 tatggattaa tccaatcgac tgatatgttt agtaaataaa gaaagaggaa tgtcaatgat
    22081 gaaaaaagaa atcgtattaa cagcagcaat gatgttattc agcatggtag caagtacgac
    22141 ttttgtttct gcaactgaag tatacccaaa ggaatataac acagaaggaa cgattacatt
    22201 tgaagcagga gatgagggtg taactcctcc tgttgatcca gagaatcctg atcccaataa
    22261 acctgtcgat ccatcagatc ctccaagccc tggaacagga ggggcattat cgattgatta
    22321 tggatcaaaa ttcaaatttg ggactcaaaa aatttctact gcggataaaa cgtattatgc
    22381 agctgctgat gtgatgaacg atgggtctag aaaaccgaca tatgtccaag tgacagatag
    22441 gagaagtacc ttatctgggt ggaaattaag tgtctctcaa ccagaacagt ttaaaactgc
    22501 cagtggggat gaactagttg gagcacagct gaaatttacg aaagggcagg ctgtttcttt
    22561 ggttgatcca acatatacgc cgcaaacggt gaattctgaa ttaacgttga caccgggagg
    22621 aaacaatacg ttagcaatta atgctaaatc aggaacaggt gtggggactt gggtctatcg
    22681 atttggtgca aatgaaaatg aaaatcaaga tgcggtacag ctttcggtac ccggtaaatc
    22741 tgtcaagtta gcgcaacaat attccactaa attagtttgg acattagaag atacacctaa
    22801 caactagttt actgaaaaga actgttaaaa ataaatacgt agtcatattg tcttgcgagg
    22861 taatcgagca aaaattgtca aaggctaggc taaatttgtt tggatacgaa tagttcatct
    22921 aggaatttaa cgatcaggag gaaaagatat attgaaaaag aggaggatcg tgctcatttt
    22981 aagcagtctc ttagtgacta atatctttcc gatcataggg tactctttgg aattgaatca
    23041 gttggagcat ggaaatagaa gagacgaaga aagcaaactc acttcgacat tcgacagttt
    23101 aatagaatcc aaccagacac aagaaagcag tgaggattta agtatttcga gtagtggaga
    23161 gaccactgac aacgtagaag ctgagggttc ggacaaacaa ggactagaag atgcaccaat
    23221 ttctgcggtt gagctgcctg acgatcccaa tattatttct atcgataaaa tttttcaaac
    23281 acctatcggt actagcacgt ccatattgga aaatgggaag ttactgcagt tgaacccagc
    23341 agaaaggtct caaagaggag ctatttggtc aaataagaag attagtttac tctcggattt
    23401 tacatttaaa tcttatttat acttaggaaa tcaacgagat agagcgggag atggcatgac
    23461 ctttacattg actggcgatc ctagaatggc tagtaatccc agtcaagtta ttggatcgcc
    23521 aggtatggga attggctcat attctactcg ttctggccaa ccttacattg cgaatgcgct
    23581 ctcgattgag tttgatacgt ataaaaatag tggttcttct gatcgaatgg atcgagagat
    23641 aactgctgat aatagagggc atggacatgt cgcttttgtc acgcctaaac caaataataa
    23701 taattattct ggagagcata ctggcgtgac ggtagctcct acatttcttt ctaatggcac
    23761 atggcgcttg ttaataatta aatgggacgc ggagcaaaga agtcttactt atgatttgga
    23821 aggtgtcggt cgctcttctc ggattatacc tgatctcatt aggcagtttg gttctacaga
    23881 agtaaattgg gggtttactt cttcaacagg tacttttttt caagaaaatg ctgtcgctgt
    23941 cacgcgactt ccatcgaatg tcacatcaac agctgatgta aaagtcaacg atagtgatta
    24001 tggggccatc gctgaagctg gaggaaacga taagataact atcagaaata cattgaaggt
    24061 cgagggggat tttcttgaat ataagaaaat gcagactagt atcgacctcc cacaagaatt
    24121 aactattccc gagcatatct tgatcaatgg aaatcaaatt gatgccaaag atatcttgat
    24181 aaccggaaat cgagtcacgc taaatctgac agattatttt attgctggag atacgttaat
    24241 aatcgaattg gagacaactt tgcaggaagt aattcctgaa aaagtactca ctatgcattt
    24301 tgaatacctt gaaaacggga atttgattga gcaatcgaat gaaataaacc taaagatcgc
    24361 aaagctgaag gagatagata tccatgttta ttataaagat aaagacagtc agattgatat
    24421 tgctgaaatg aaaacaatca ctgggaaaat aggagagaag tataaagaaa ctcctattga
    24481 tattgaaggg tatgtttttg actcggattc agcgaataca gaaggcatct tctccgaaaa
    24541 tacagaggat atctattttt attacagaaa aggcaaactt tatctctcaa ctgctcctca
    24601 atatgtcaat tttggaaaac ataaaatatc cagtagacca ttaagtgaat tcggacagtt
    24661 ttcaggtggg ttgaaaatta tagatgaacg ggcttttgga agttggaaat tacaattgaa
    24721 acaagagaaa gttcttacaa atggagattt tgagcttcca gaagctcttt cctttgttac
    24781 ctctgaaaat acaacggtta tagggacatc cgctgttacg attttcgaaa gtgatcaaaa
    24841 gggtgaaagc gatttgtcct ctcttttaga ttctacagct catcgtggaa tcagaataaa
    24901 tattcctgta gaaaaccaaa gaatcggaac ctttgagggg aaactatctt ggatacttgc
    24961 agatgtccca ggaaattaac aggaagagaa gaatataaca aaattattga gtttcaagaa
    25021 gggcacaggt accataggct cttgtgtccc acagagacaa gattttgtta tagtcttctc
    25081 ttacatatga ttaaaggagc gattttcttg agaccaaaac aacaattatt tataacactc
    25141 ctagttattt taggggcact ttttaacaat atatcagtgt cagctagtgc atttaatttt
    25201 tcagtgacgc cgataacttc agagaatcaa atcgacaaga gaaaaactta cttcgatctt
    25261 caacttgatc cggatcagga agtagaagta aaagcggaat tgagaaatga tacagaaaaa
    25321 gaagtgaaaa tcgatatttc tgtgaatagc gcaaccacca attcaaatgt gatggttgaa
    25381 tacggaaaaa acgagattga aaaagacatg agcctgatct ttgatctggt agattatgtg
    25441 agctatccac agatagtgac gctaaaacct aaaagtgtcc aaaccgttgc ttttcatgtc
    25501 aggatgccga atgagcgatt tgatggtgta ttggctggtg ggatcacatt ccaagaaatc
    25561 cagacggaaa aggaccaaac agaaactaag gatcaaagct tatcgattga gaatgaatac
    25621 acctatatta ttgctttgtt gatgcaacag acactgaacg aagttgctcc gaaccttact
    25681 cttcatgaag ttaaaccaga tcaaatcaat gcaagaaatg tcatcttagc aaacgttcaa
    25741 aatgatcaaa aaacctatat caatcaagta gttattgaaa caaaaattac aaaaaaagga
    25801 cattctgaag ttttatatca agaggaaaaa gaaggattgc agattgcgcc gaatacaaat
    25861 ttttcttttc caactgcttt aaatggtcaa ccgctaaccc caggtgaata tcaccttaca
    25921 atgactgttt taggaaatga aaatgaaaat ggaaaatttt cgagaaaaaa aggaaatact
    25981 acgatcaact ataccaatca atgggtattt gaaaaagatt ttacaataga cggaaaagta
    26041 gccaaggaat tgaacacgac ggatgttacg attaaaaaag acaataattg gatttatatc
    26101 ttagtagctt cacttttctt attgtttatt ttattatgca tacttttgtt tgttttgaga
    26161 aaaaaagaag agaaagaaaa gtaaggttaa gataacaaat agtaaatttt tattattgta
    26221 ggatggttgg gctcttttta taacaatata aaatatacca ttttattaat aaaaatgcat
    26281 actagtaaat gatataattc taataacagg tttgatcgcg agaaaggcca aataaaacgc
    26341 tctccaaaat gttaaaaaaa agacaggacg atgtatatta tacaaacaag aatcgctgac
    26401 aacagtgact cggatccagg aaatcagttt tatccccagc tagttggtga atgcatggaa
    26461 agtaatatac gtagaaagag ctgatgacta atgaattatg agtttttttt aagcaaagaa
    26521 gatcacagaa gttattcatt acttaaatat ttagaagcaa gctcaaattt atcagagtca
    26581 atatcgaatg ttcaagaaga gttgtcgcta tcgacatttc tcttgaaaaa aacgatcgat
    26641 aagctgaaac atgatttaca aaagttcgat ctagagaaaa atttgcgagt aaccgtcagt
    26701 gaattggata tatgtctgga aattaatgga aaatgttcta gcaaagctct gttatctcgt
    26761 tatataacgg attctttatc tatgaaaatg gtattggctt tctttcagga aaaatatatc
    26821 tcagtagaaa attttgctaa acagcatcac gtgagttatt ctgtggctta taaagtatta
    26881 caaagactaa aaagaaatct aaaaaaatat caaatttttt ttgaaaagag aaagttgacg
    26941 agagtataaa atattttgtg taaatgaaaa aatccataca aaaaaggaag tcccttctgt
    27001 agaataaagt taacgacaac caattcacag aaaagaggac ttccctatga atgattttac
    27061 tacagaaatt gtgcaaactc tagtcactaa aggcgattta aatgaattat tccgttcgca
    27121 cttagaaaaa gcgataaaca cactcctacg gactgaatta acggcttttt tagattacga
    27181 aaaatatgat cgcactggtt ttaattcagg taattcgaga aacggttctt actttcgatc
    27241 aatcaaaacc gaatatggtg aattaacatt ggaaatacct agagatcgta atggtgagtt
    27301 taaacaacaa actttaccag cctacaaaag aacaaacgat acattggaaa ccactattat
    27361 ccatttattc gaaaaaggtg ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta
    27421 cggtcatcac tatactccac aaaccatgtc caacatgact aaagttctga ctgaagaagt
    27481 aaatgccttt aaatccagag ccttaaatga taagtatgtc gctattttta tggac
//
LOCUS       DASJSZ010000034        25571 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.034, whole genome shotgun sequence.
ACCESSION   DASJSZ010000034 DASJSZ010000000
VERSION     DASJSZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.034"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            220..774
                     /locus_tag="U4B03_001332"
     CDS             220..774
                     /locus_tag="U4B03_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2068193.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(947..1420)
                     /locus_tag="U4B03_001333"
     CDS             complement(947..1420)
                     /locus_tag="U4B03_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2068194.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKIKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1432..1968
                     /locus_tag="U4B03_001334"
     CDS             1432..1968
                     /locus_tag="U4B03_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2068195.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2008..2379
                     /locus_tag="U4B03_001335"
     CDS             2008..2379
                     /locus_tag="U4B03_001335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2068196.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2663..3934
                     /locus_tag="U4B03_001336"
     CDS             2663..3934
                     /locus_tag="U4B03_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2068197.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4179..5204
                     /locus_tag="U4B03_001337"
     CDS             4179..5204
                     /locus_tag="U4B03_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2068198.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5498..6607
                     /locus_tag="U4B03_001338"
     CDS             5498..6607
                     /locus_tag="U4B03_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN2068199.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6690..7586
                     /locus_tag="U4B03_001339"
                     /pseudo
     CDS             6690..7586
                     /locus_tag="U4B03_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7602..8426
                     /locus_tag="U4B03_001340"
     CDS             7602..8426
                     /locus_tag="U4B03_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2068200.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8458..9768
                     /locus_tag="U4B03_001341"
     CDS             8458..9768
                     /locus_tag="U4B03_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEN2068201.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9916..11175
                     /locus_tag="U4B03_001342"
     CDS             9916..11175
                     /locus_tag="U4B03_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2068202.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11185..12057
                     /locus_tag="U4B03_001343"
     CDS             11185..12057
                     /locus_tag="U4B03_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2068203.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12069..12899
                     /locus_tag="U4B03_001344"
     CDS             12069..12899
                     /locus_tag="U4B03_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2068204.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12939..15122
                     /locus_tag="U4B03_001345"
     CDS             12939..15122
                     /locus_tag="U4B03_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN2068205.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15183..16403
                     /locus_tag="U4B03_001346"
     CDS             15183..16403
                     /locus_tag="U4B03_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEN2068206.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16400..17860
                     /gene="gtfA"
                     /locus_tag="U4B03_001347"
     CDS             16400..17860
                     /gene="gtfA"
                     /locus_tag="U4B03_001347"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEN2068207.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17937..19043
                     /locus_tag="U4B03_001348"
                     /pseudo
     CDS             17937..19043
                     /locus_tag="U4B03_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19365..19574
                     /locus_tag="U4B03_001349"
     CDS             19365..19574
                     /locus_tag="U4B03_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN2068208.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19567..19716
                     /locus_tag="U4B03_001350"
     CDS             19567..19716
                     /locus_tag="U4B03_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEN2068209.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19870..20535
                     /locus_tag="U4B03_001351"
     CDS             19870..20535
                     /locus_tag="U4B03_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2068210.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20819..21904)
                     /gene="xerS"
                     /locus_tag="U4B03_001352"
     CDS             complement(20819..21904)
                     /gene="xerS"
                     /locus_tag="U4B03_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEN2068211.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22238..22471
                     /locus_tag="U4B03_001353"
     CDS             22238..22471
                     /locus_tag="U4B03_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEN2068212.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22468..22875
                     /locus_tag="U4B03_001354"
     CDS             22468..22875
                     /locus_tag="U4B03_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN2068213.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24012..24530)
                     /locus_tag="U4B03_001355"
     CDS             complement(24012..24530)
                     /locus_tag="U4B03_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEN2068214.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         8708 a         3903 c         4766 g         8194 t
ORIGIN      
        1 gtctaaaatt taaataagag cttagaatct tcatataagt aaggttaatt tttttgtaca
       61 aaaaagtcgg aatattctga gttactgaaa tgatataaaa tcataaacta aatcccttga
      121 ttttattgac tttcaaaagt ttatatatat aagttaatga ttatggtaaa atatatacat
      181 aatcattaac tcagaatatt tttaagaagg gaaacaatag tgagctataa tgtacaaccg
      241 ttaaggacaa atgaagagat acaagatttt cagttttggc taagaagaac aagtaatccg
      301 gaaagagata gttttttatt tttgtttgga atcaataatg ggttacgtat gtcagatatt
      361 ataggattaa aagttggaga tatacgaggc aaaagtaagc ctattattgt tgaacataaa
      421 acaggtaagc gaaaaccgat ttttatagat aatttgagag aagaaatttt attatatact
      481 gaaggtaaag aagaaaatga ttggttattt ccgagtcggc aacaaggtcg acatataaca
      541 agggatcgtg tctatcagat atatgcagat atagcagaaa aattaggacg agatgatata
      601 ggaacacata cattaagaaa aacttttggg tatcactact acaagaaaac tagagacatt
      661 gctaccttaa tgtttatttt taatcatagc tcgcaagcaa ttacaaaaag gtacattggt
      721 ataactgaag atgaaatagg agcatcactt cgtggattta aactaggtgt ataatttaaa
      781 aatttttata aacagtaaag acaaagatta aaagctattt ttatctagaa cgattttatt
      841 tcataaagtt cgcggaataa aagctggggt tactggtact tggtataggt tcgtgttata
      901 taaactaagt aattaaataa catttttagg ttggaaaagg atgaaattaa aaaaagagaa
      961 tcaatacggt ctcaaaaacg caaacgctgc ggaataagaa aaatactgat aactcaacgt
     1021 ttagcagact atctaatagg gatagtctgc ttttttcgtt tttgaccata cttttgatct
     1081 tctgcaaact tccttgcttt tcctctgtag aactcatgaa atctgcaaaa cgaattgccg
     1141 tttcctctgt ctgctttttc gcgatatggg tgtatatatc atccatagtg gttttaatat
     1201 ctgtatgtcc aagtcgaaca tacacttctt tgacagacgc accagattca aagagcagac
     1261 tacaatgggt atgacgaaaa ccatgaagtg tgatatgttt taagttattc tctttaatga
     1321 tgattttcaa attgtgattt acatagtcta aataaagggg cttattttct tctgatgaaa
     1381 acaaaagttg ttcaccatct tttataggtt tgattctcat ttttagatat tatgcccgca
     1441 cgtattcatc acatttctat tgtgaatcgc tttattcgac cgacatttga cttttatcat
     1501 aatatactcg gtttaaaatt actaatgaaa acaattaatc aagacgatca taccatgtat
     1561 cacctgtttt tttcagataa tcaccaccgt gtgggaactg aactgacttt ttttgaagta
     1621 caagaaggga acaaccagtt ttttggaacc aatacgattg aacgaacgat actaaaagtt
     1681 ccttccgaag catcattgca tttttgggaa atatattttg aaacacaagg cgtttgccat
     1741 tatggaatcg aatcattttc tggacgacct atcttacgtt ttgaagggcc agatgcaaca
     1801 cgattagctt tagttccctt acgcgaattt gaagatattg atgattattt cccgtatgtc
     1861 acagaccaga tccctacaga gcatgccatt ttaggaattg atgccattca actacgcgta
     1921 caatattccg atgcgactga acgagagtta ttgtcggtag gctggtagca caaagaatac
     1981 gtcagtttct tttcgggtca tcaacgagtg acaatcttag aaaatcaaga cactcagttc
     2041 tatcaagaag ttcagattat tcaagatcgt gagaatccag ttgccattga agggattggt
     2101 agcgtccatc atgttgcgtt tggcgtagaa aataaatctg atttacaacg catcgacaaa
     2161 caattacaag agcgcaattt tataaattca ggaataaaag atcgtgaatt ttttgtatca
     2221 ctttattatc gtgacccaaa tcaattattg attgaaattg ctacagggga aggaaaccta
     2281 gatgcaaaag cgtatgaaaa tcaaagtcca cgctttgaag aaattccttt atttttaccg
     2341 cagtacttaa atttcattcg tgaacaagtg gagcaataat tagctaatca aatccatgac
     2401 gaagagtaag aggttgactc ataagtcaaa aaataaagaa aaacgcatca aatccattct
     2461 aaagaatgtt gatttgatgc ggttcctatt tttataaact atcattttcg actaataaaa
     2521 ctacttatga gtcagcctct ctttttttct tgatttttat acctctttct aggtaaacta
     2581 aacatctgcc aaaaataccg tgtctcattg aaacattctc aactatttca aaataaaggg
     2641 gagtaccaaa tgaacgagta tattgaaatt atccacgcaa gagaaacaac ttaaaaatgt
     2701 cagtctcaaa attcccaaaa ataaaattag tatttttacc agcgtatctg gttcaggaaa
     2761 atcatcgatt gtttttgata ctattaccca agaagctggt cgtcaactga atgacaccta
     2821 tgatagtttt acccgtttat ttttacctaa atacagtcgt ccagaggttg atgagattga
     2881 taatctttct acggcgattg tggtcaatca aaagaagatt ggtggcagtg cgcgctcaac
     2941 gttagggacg attactaaca tcaatgcgtt atttcgtgtg ttgttttcat gttttgcaac
     3001 acctagttta ggatatgcaa atgcttattc atttaatgat cctagtggaa tgtgttctgt
     3061 ctgtcaggga atcggtaaga cgattgcatt tttaatagga ttgctttatt tttcggctat
     3121 tgataatgct gattttgtat tttctgatcc atttaccgca gaagttttgt ctggaattga
     3181 ggaacaggca aagaatcatg gttattttgt attggtgcat aatgttaatt cagcagaaga
     3241 tgttaaaact attcagagaa attggcgttt tgaggggttc attgttgttg gtgtacgtgt
     3301 aaaagaattt ttggaattaa ataacgtttt tgatggtcct gttgtttata ttgatacgca
     3361 tctttcaaaa gagacattaa gagaacttga acaacaaatg aatcgtttat ttgtaaatac
     3421 aaatgattac caagctagta cattggcagt ggaacatttg attcattatg gtcatgagaa
     3481 aatagcgttt ctttcttttg actacattgt aggtgaacca agtgttattc aagaacgaca
     3541 tcaagcttat atcgatacat taaagaaata tcatttaacc tttaaatcgc attggatata
     3601 taacaactct gaatttgagc gaatttatca agaattagct gaatttactg caattgttgt
     3661 tactggagat tatttagcgg ctaaattagt aaaattttta aaaaataaga ataaacgagt
     3721 gatggaacaa ttatcgatta ttggatttga tgatattact tttgcagaat taatggatcc
     3781 tgcgttgaca acaatacgac taaatccagg taacaagggg gcgattgctt ttcaccaact
     3841 gattggactg gccaaacaag aagaaataaa aaatcaagta ctactattag agggagaatt
     3901 gatttctcga gactctgttc acaaaatcaa ataaaaaagc tctgtgaaat aagtatagtt
     3961 aacaataaaa tatccgaacg atagagagaa tgaactgtga tctagcagtt cctcgtcgca
     4021 aattcactag aattagcatt atttactcta aattgttcgg atattttttc ttgtttttaa
     4081 ttatgattac agtgcctatt tttaacgaaa tttgcttatt ttcaaaatgt atgattatat
     4141 gttagtaaac actttcaaat atgaaaagag gagctattat ggtaggaatt agggatgttg
     4201 caaaacgggc gggtgtgtcc attgcaacag tatcgcgcgt gctaaatgag gatccgacac
     4261 tttcagtaac cgatgaaacc aaacgaaaga ttgcagaatc ggttaatttt tatcattatg
     4321 tcaaaaaaag tcccgttgca aaaaatttaa caaatattgc attaataacg acagtttctg
     4381 aagtagatga gttggaagat ccctattttc gaacgattcg cagagggatt caagtcgaag
     4441 cagaacaaag caaagtcaat ttgaagaaaa ttattcgttt gtcagaaaca ccgttggagc
     4501 aagacgattt gctaaaatgt caaggtgtgt tgattattgg gcaggtgcta tcttctgtga
     4561 ttgaagaagt atcagcaatt aattcgaatg tcgtagtgat tgatgaccct agtattgaag
     4621 ctgaagtaga tgcagtttat actgatttaa caaaagcaac gaatcagcat ttagatcgat
     4681 tgtataaaaa aggacaccga aacattgcgt ttatcggagg aggacgtgtt cgtttagata
     4741 gccaaggaaa taaacacgtg tcagatgatg attatcgaaa aatagcctat gagacgtgga
     4801 tgaaacaaaa aggattagaa aaatatagtc aaatttattt agaaggctgg accacacttg
     4861 acggaatgca ggcagcagat ttacttttga aaaaacatgc taaagataag ttgccgactg
     4921 ctatcatggt aggaaatgat cctattgctg tgggggttta tcgttcactt caaaaaaatg
     4981 gtctacatat tccagaagat atttcgattg tgagttttga taatattgaa gttgctgaat
     5041 ttttaacacc tgcattaaat acggtgaatg tgaatacaga agaaattgga aaactagcag
     5101 ttcgcatggt taatgaacga ataaaagaag ttagaaatgt ttcgatcaaa gtgattgttt
     5161 ctaataatat tattgttaga gaaagtgaaa aaaatatcga ttaaaaaagt aaacatccaa
     5221 aaaagggtgt ttactttttt gtttactaat atttttagta aaaaatctcg acaaaacatt
     5281 aagtaaacag ttatggtgtt gttaacaaaa agaaaacgca ttcaaaacag gaggaattaa
     5341 gatgaaacat ttcaaaaaaa tagctttaac agggatagct gttttaactg ggacattact
     5401 cgcagcatgt ggtgggggag atagtaattc taaagacggg caagtaactt taagctatgc
     5461 gagttgagat actgaccaag ctccaggact aagaaaaatg ttagatgaat ttgaaaaaca
     5521 aaatcctgat atcaaagtag aaatggagac aacgccttgg gatcaatatt gggttaaatt
     5581 agaagctgca acaacgggag gtaatatgcc agatgtagta accatgcact ctagcgaatc
     5641 ctataaatat atgtcacaga atgctttgat gggacttgat gatgttatca aagaaaatga
     5701 tattgacatg ggaaacttta ctgaaggaat tgctgatttc tatacgcttg aagacgaact
     5761 atacgcaatc cctaaagatg cttctgtagt ggggctatgg tacaacaaag aattatttga
     5821 taatgcaggt gtcgattatc cagatgaaac atggacatgg gaaacactca gacaagctgc
     5881 aattgattta acagatgaat caaaaggcat ctatggtttt gcatcacata ttggaacaga
     5941 gactggctac tggccattta tttatcaaaa tgggggagaa gtatttgccg atgacaatac
     6001 aaaatcaggt ttgaataccc cagaagcaca agaagcttta gctttttatc gagatattgc
     6061 acttgttgat catgcttcac caacaattac agaattacaa gatcctgata agttaagtcg
     6121 ttttcaatca ggtagattag caatgatgat tgaaggaaac tggtttgcac cgggattttt
     6181 gaaaaatgac tatctagttg aaaatgttgc ttctgccgtg ttacctaagg gaaaagttaa
     6241 tgcaacaatt acaaatggac ttggttgggc tgccagtgcg agtacaaaat acccagaaga
     6301 agtgaaaaaa ctaatggcat ttttagctag tgaggacgcc aataaaatcc aagcggaaac
     6361 gggggcatca atcccagcac taaaaggctt tgaagacgag tgggctgctt cttcagatac
     6421 aattgatttg agtgcttttg gaaaaatgtt ggagtatgga gtccctcgtc catacaataa
     6481 agatggatta cgagctgaac aagcagaagt tcaaattatt aaccgtattc tgtctggtga
     6541 agtaactgtt gaagatggaa ccgctcaaat tgaaaaagaa gtcaatgcga tattgagcgg
     6601 aaattaaatt agttaattat acaatataca ggcgaaagga ttctatttcg tctgtatatt
     6661 aatggatgaa taccaaaaag ggggatatta tgaaaaatcg aatttttgga aaatatcgta
     6721 aagatcaagt gatatggggc tatattttta tttttccaac ggcattagga ttaattttat
     6781 taaatatatg gccagcaatt caaacagcaa ttttaagttt tcaaaaaacg gtagggttcg
     6841 gtgatactga atgggtcgga ttaagaaatt accaaaaaat gtttgcagac ggtgaagttt
     6901 ttcagtcttt attaaacaca ttaatttatg cagtggtttc gatacctagt attgttgtat
     6961 tatccttgtt ggtggcagtt ttaatgaata gaaaagttaa aggattgtcg atttatcgga
     7021 caatttattt tctaccagtc gttgctgcac catccgcagt cgcaatgatt tggcgctgga
     7081 tgttcaataa tgattatgga ctgattaata acatgttggc taaaataggt ctcgatggac
     7141 cagcatggtt aacggatcca tcgattgcga tttattctat tatcattgta ggagtttgga
     7201 gtgctattgg ttataacatg gtgttattac tagccggctt ataagaaatt cctaaagatt
     7261 attatgaagc agcggaaatt gatggtgcta gtccaatcca gcagtttttc aacattacgt
     7321 taccattagt tacaccaaca ttattttttg tagttgtaac aacagttatc aatgctttcc
     7381 aagtatttga tgttattttt atgatgatta ctcctaatag taccgccatg tttaaatcac
     7441 aatcattagc ctatttattc tataaacatt catttatttt aaatgacaag ggatatggtt
     7501 cggcaatcgt tatgttcctt ttagtgatta tattaattat tactgcaatt caaatgaagt
     7561 tgcagaaaaa gtgggtaaac tattaaggag gctatcaaat tatgaaaaat aaattttttt
     7621 caaatgcgcc aatccatatc atgcttattt taggtggtat tgcaatggca ttacctttta
     7681 tttggatgat tttaacttca gggaaaacgt tggttgaatc tacccaaatt ccacctacaa
     7741 ttttccctga gaaatttcag ctaagtaact atcaacaagt acttgattta ttaccattca
     7801 tggcttttta tatcaataca tttttaatgg ttgttggacg agtgattggc tcggttttat
     7861 tttcagcaat ggctgcatat gcttgtgcgc gtctaagttt tccagggaaa aatatccttt
     7921 tcagcttagt acttattcaa atgatgattc ccggttccat ttttattact ccacagtatt
     7981 tattagcaca aaaattgggt attttaaata caattccagc attaatatta ccaggggtag
     8041 ttagtgcgtt tggtacgttt ttattaagac aatttttctt aggtttacct gatgaattgg
     8101 aggaagcggc ctatttagaa ggtgccaata tttggacgat ctttttcaaa attatgttgc
     8161 cgcttgctaa atcaggattg gttgcacttg gaatattcac agcgttgttt gcatttaaag
     8221 atttaatgtg gccattgatt gtcaatactt caattgataa gatgccttta gctccgggat
     8281 tagcaaactt acaaggacaa tatgccacga acttcccaca attaatggca ggttcgttat
     8341 tagcaatttg gccaatagtt gtgatattca tcgttttcca aagaaaattc attgaaggaa
     8401 ttgctacaac aggtagtaaa ttataaaact attaattagt gattggagag taagtacatg
     8461 acaaaaatta catttattgg tgcaggaagt actatttttg caaaaaacgt attaggcgat
     8521 gcgatgttaa ctccaagtct acaagatgca catattgcat tgtacgatat tgatgaaaaa
     8581 cgactaaaag aatcaaattt aatgcttaaa aatattaata aaaattcgaa tgaaaatcgt
     8641 gctgatattg tttcttatta tgatcgcaaa gaagcgttaa gagaggctga ttttgttatc
     8701 aatgctattc aagtgggtgg ttataaacct tcaacagtga ttgattttga tattcctaaa
     8761 aaatatgggt tgcgacaaac tattggtgac accatgggga ttggtgggat ttttagaacc
     8821 ctacggacac taccagtaat gttagacttt gctaaagaaa tggaagaagt ttgtccaaat
     8881 gcttggttat tgaactatac aaatccgatg gcgatgttaa ccatgggtgt tttaaaagcg
     8941 acgagtatta agactgttgg tttatgtcat agcgttcaag tatgcgtacc agaattgttt
     9001 gagcatttag gaatagccga tcaatatgac ttaaatgact ttcaatggaa aattgcaggc
     9061 attaatcata tggcattctt gttggaaatt acaaaaaatg gggaagattt ctatccaaca
     9121 attcgtcagt tagctgcaga aaaaccaaat ccacatccag attctgtacg ttttgaattg
     9181 ttgaaacgtt ttggttacta tattacagaa tcaagtgaac acaatgcaga ataccatcca
     9241 tactttatta aatcaaaata cccagaattg attgacgaat tagggattcc gttggatgaa
     9301 tacttacgtc gttgtgaaca acaaattaat gattgggaaa gcatgcgaga ggacattgta
     9361 aataatgggg agttaacaca tacgagaagc cgtgaatatg cttcatatat catggattct
     9421 attacgacag gaactccgtc agtgattgca ggaaatgtct taaataaagg cttaatcaca
     9481 aacttaccga cagatgtttg tgtggaagta ccttgtttag tcgataaaaa tggcgtgcaa
     9541 ccaacctatg tcggtgaatt gccaacacaa ttagctgctt taaatcggac gaatatcaat
     9601 gttcaagaac tgacagtaga agccgcttta acactagata aatcaaaaat ttatcaagca
     9661 gcgtatttag atccacatct atcatctgaa ttgtctttaa cagatatcaa aaatttagtg
     9721 gatgatttaa tcgaagcaca tggggactat ttacctgaat ataattaaaa agaagctgaa
     9781 agagtaagga attttcaatt ccttactctt ttcttagtta aatttagtaa aaatattgac
     9841 aaaacactta gtaaacagtt acattagtgt taacaaaaag aaaacgcatt caaattgtat
     9901 aaggaggagc ataaaatgaa gtggtctaaa aaaattattt tagcttgtat agcactagga
     9961 gcaacgatct ctttgactgc ttgtggaaaa aatcaggaag atgcaagcgg taaggtagaa
    10021 atagagtttt ttaatcaaaa aaaagaaatg actcaaacga ttcaggagat tgctaaagat
    10081 tttgaagcaa aaaaccctga tattcatgta aaagttgtgg atgtaccgaa tgctggtgaa
    10141 gtaattaaaa cacgtatgct tgcaggcgat gtaccagatg tcattaatct ttatcctcaa
    10201 agtatagaat tgaaagaatg ggcgaaagct ggttatttag aggacttaac cgaagaaccg
    10261 tatttagaga atatcaaaaa tggatatgca caacgttttg ctattgagga tcgtgtatat
    10321 agcataccat taaccgctaa tgtctatggt ttttactata acaaaactgc ttttgaggaa
    10381 atggggattc aagcaccaga aacttgggca gaatttgaaa aaatagtagc tgagattaaa
    10441 gatcaaaaca aagtaccgtt tgcgattgca ggagcagaag gctggacttt aaatggttat
    10501 caccaactag ctatagcgac tgctgctggt ggtgaagagg aagcgaacaa tgtttggcgt
    10561 ttctcagaag ttaatggaat taatgctgaa agcaaagaaa tgcaaaaaga ttttaatcga
    10621 ttagatttgt taagagaacc aaaagctttg caaaacaact ggcaaggtgc cggttataac
    10681 gatacagtag ttacttttac aaaaggtgat gcgttgatta tgccgaatgg ttcatgggca
    10741 atgccgatga ttcattcaca aaatccagat tttgaagtag gaacttttcc ttttccagca
    10801 gatgaagcag gtcaaagtct aacgattggc gcaggagacc tagccttatc agtttctgca
    10861 acttctcaac ataaagaaga agccaaacgt tttgtagaat atatgacaac acctgaagcg
    10921 atgcagaaat attatgatgt agatggtgca ccgtgtgctg ttgagggcgt tatcgagaat
    10981 actcaggatt caccattaag tggtttaact gaacttgctt ttactgatcg tcatttagtt
    11041 tggttagcca aagattggaa tagtgaaaat gatttttata ccttaacaac aaattattta
    11101 cacaatggca atcaaaaagc catgattaac gcattaaatg ctttctttaa tccaatgaaa
    11161 gctgacggaa ggtaggaaaa taaaatgaaa ataaaagaaa ttttaaatcg ttactgggca
    11221 atcatttttg tagcaattcc aattattttg cagatgattt ttttctactt tccaatggtt
    11281 caaggtgctt tttacagttt aactaattgg actggtctga cttataattt tgattttgtg
    11341 ggtttgaaca attataagat tcttttttct gatccgaagt ttatcaaatc gattggcttc
    11401 actttaattg tgacgctttg catgattgct ggacaaattg gttttggtat tttgattgcg
    11461 cgggcgttga acgcaaatat taaagggaaa acattcttta gagcatggtt cttttttcca
    11521 gcggtcttat cgggcgtggt tgtttcttta gtatttaaac aaatttttaa ctatggttta
    11581 ccaaaaattg gtagtatgtt aggcattgaa tttttacaaa ccagtctttt aggaacaaca
    11641 tggggagcga tttttgcagt aatttttgtt ttactttggc aaggaattgc catgccagtc
    11701 atcattttct tagcgggttt tcaaagtatt ccacaagaaa ttattgaagc tgctagaatg
    11761 gatggggcga atgataagca aattttttgg aatattgaaa tcacctactt attaccaagt
    11821 atttcaatgg tatttattat ggcattaaaa ggtggcttaa cagcatttga tcaaattttt
    11881 gctttaactg gtggtggacc aagcgatgcc acaacatctt tggggttact tgtttataat
    11941 tatgctttta aaaacaatca gtttggttat gcgaatgcta ttgctttagt cttatttatc
    12001 ttaattggga ttgtttcact ggcacaatta aaattatcga aaaaatttga aatctaggag
    12061 gtttcccaat gaaaacatca aaagaaaaaa atatttctaa atacttgctt ttattattag
    12121 ggggcttcat gattttagtt cccttagttt caacgctatt cggttcattt aaaacaacac
    12181 ccgatattat gaataatttc tttcgttttc ctaatccagc gactttggaa aatttccgaa
    12241 gattgttagc ggatggaatt ggtcattact tttggaattc ggcaatcatc acaatcattg
    12301 cagtagttgc tgtgacagca attattccta tggctgctta ttcactagca agaaaaatga
    12361 ctcgtgggaa agcatacgga ttgatgtatt tatttttaat tttaggaatt tttgttcctt
    12421 tccaagtaat tatgattccg attaccgtta tgatgagtaa aattggttta gcaaatatgt
    12481 ggggattaat cttactctat ctcacttatg ccgtaccaca gacactattt ttatatgttg
    12541 gttttattaa actaaatgtg cctatcagtc tggatgaggc tgctgaaatt gatggggcta
    12601 gtcactttac aacttatttc aaaattgttt tcccaatgtt aaaaccaatg catgcgacga
    12661 ctattattct taatgctcta tggttttgga atgatttcat gctaccatta ttaattttaa
    12721 atcgtgattc taatatgtgg acactaccat tgtttcaata taattatacg ggacaatatt
    12781 ttaacgacta tggtccaagt tttgcttcct atacagtagg aattgtcacg attacactag
    12841 tttatcttgc tttccaaaga agtattatag ctggaatgag caatggagca gtgaaataac
    12901 caataaatta ttctcattat aaaggagttc ataaacttat gattatattt gacaatcaca
    12961 cacaaacatt tcatttacaa aataaaaaaa ttagctatct attaggaatc gaagaaaata
    13021 attatttaac gcatctttat tttggaaaaa agattacgca ctattctggt gggtatcgct
    13081 acccccgaac agatcgttcc ttctcaccca acaatgcaaa tgcgcaagat cgtttattat
    13141 ctttagacac attaccatta gaatttccag gatatggaca tggtgatttt agagaaccgg
    13201 cgcataatat taaattagga aacggtagtc gtgtaaatga ttttcgttat gatagctacg
    13261 aaattcaaaa aggaaagccc agcttagcgg gattgccagc cttatatacg gaagatgatg
    13321 atgaagctga aacgctgatt attcaattga aagacgcgat tagtggattg actttacagt
    13381 tgttctacac aatttatcaa aatcacagtg taattattcg ttctacgcaa ttagtaaata
    13441 atagtgatga aacagtggag attaaccgct tagctagtca atcaattgat tttccaaatc
    13501 gtccactcga gttaattcat ttaaatggta cctgggcaag agaacgtcaa atgactagag
    13561 aaaaaattca tacagggata aaagtattag atagtaaacg cggatcaagc agccatcaac
    13621 aaaatccgtt tgttgcttta gtagatgcca aaacagatga atttcaagga gaggcatatg
    13681 gatttaattt agtttatagc ggtaatcacg aaacggtgat tcaaaaagat ccatttgacc
    13741 aaacacgggt cattatggga attaattcat ttaattttgg ttggcaacta aaaactggcg
    13801 actcatttca aacgccagaa gtcgtgatgg tttatagtaa tgaaggtctg aatacgttat
    13861 cacagacgta tcatgaattg tataatcacc gtttaattcg tggaaactat cgtttaaaag
    13921 aacgtcccac gttaatcaac aactgggaag caacgtattt tgattttaac gaagaaaaaa
    13981 tcatgaatat tgtcgatgat gcgaagaact tgggcattga aatgtttgtt ttagatgatg
    14041 gttggtttgg cggtcgtgaa aatgacttca cttcattagg tgattgggtt gaaacagaag
    14101 gcaaacttgc ttgtggtttg gaaaatttag ccaagcaagt gcatgataac gggatgaaat
    14161 ttgggatttg gtttgaaccg gaaatgattt ctgaagatag tgaactattt cgagcacatc
    14221 cagattgggc attgggtatt ccaggtcgtg accgctcaat tagtcgaaat caatatgttc
    14281 ttgatttttc aagacaagat gttcgtgata atatctatca acaaatgacg gcgatttttg
    14341 atcgtgtacc gattgattat gtgaagtggg atatgaatcg aaatatgaca gaagtctatt
    14401 ctgtcttgtt ggactcaacg atgcaaggtg aagtggctca tcgttatatg ttagggttat
    14461 atgaattttt agaaaagtta acaacgaatt atccaactat tttatttgaa agttgttcgg
    14521 gtggaggcgg tcgttttgat gcaggtttct tgtattacat gccacaaact tggacgagcg
    14581 acaataccga tgccgtggct cgtctgaaaa ttcaatatgg aaccagctta gtgtatccaa
    14641 tttcaagtat gggtgcgcat gtttcagcag tcccaaatca tcaaacagga agagaaacga
    14701 gtttaactat tcgtggcaat gctgcgatgg ctggtgtttt tggttatgag ctagatctaa
    14761 cgcaattaac ttctgaagaa aaagcagaaa ttaaagaaca agttgccttt tataaaaagc
    14821 atcgccaact tttgcaatat ggaacttttt atcgcttgga aagtccgttt gaaggcaatg
    14881 atactgcatg gatgtttgtc tcaaaagacc aaacagaagc catggtcttt tatttccgag
    14941 tattagcaga agctgcgcat ccattagtca cattgaaatt agcaggatta gcagaagaag
    15001 caacttatca aatggaagat gtaacatttg ctggcgatga attgatgaat ctcggttttt
    15061 atattgactc agaactacat ggtgattatg cgacacaacg cttttatttg aaacagatat
    15121 agtagcttga atttacaata taagtgcaac accaaaagag actcattcca aacgaagaaa
    15181 atatgtttta tttacacgca tttactaaaa agcagcctga tttgaattgg gaaaatgaag
    15241 aagtacgtga agaaatctat gcaatgatta actattggtt agataaagga ctaggcggat
    15301 ttagaatcga tgcgatatta aacatcaaaa aaaaactaga gtatggcatc tttgaatctg
    15361 atggagaaga tgggttagcc ttcattgggc cctggattct taatcagtcg ggcattgaag
    15421 tgtggttaaa agaaatgaga gagcggacat ttaaaccaca taatagtatg acggtagcag
    15481 aagccgatgt tcctaatgaa cgtttagatg aatatattgg tgaagatggc tattattcga
    15541 tggtttttga ttttagttat acggatattg atgtacctga aactggtgaa tggtttaaag
    15601 atagccaatg gacatggacg gatatgcgta ccaatatttt tacgaatcaa ttagttacgc
    15661 aagataaagg ttggggcgcc ttatatttag aaaaccacga tcaaccacgt tcaattaata
    15721 aatatattcc agaagaatgg atcaatgatt atagtaaaaa aatgttggcg acgcttttta
    15781 tgttactaag aggtacccct tttatttatc aaggacaaga actaggaatg accaacatag
    15841 aaatggcatc attagaagat tttgatgatg tagctacgca ttctcagtat aaacgggcat
    15901 tagaatatgg attggcacca gagcaagcat tgaaagctgt caataaacga agtcgggata
    15961 attctcgcac acctatgcaa tggaatacgc aaaaaaatgc cagattctca acttctgata
    16021 atgtatggtt aaaagtaaat cctaattatc cagaactcaa tgcagaagct cagttaacca
    16081 atcaacagtc ggtattcaat tattatcatc aattaattaa attaagaaaa aatagtgtat
    16141 acaaagacgt gctaatctac ggacaatttc taccagtgaa agataaaaat gaacatattt
    16201 tgctttatga acgccaatta gaagataaaa tcgtattagt tatcttaaac gttacagcaa
    16261 cagaacaaga atattatgta gatgaacagt accaacaagt attaatcaat aattatgaag
    16321 atgttttatt agatcaaagt cgaaaattgc gtttacgacc gtttgaaagt attgtattag
    16381 ctaattatgg aggacaatta tgaaaattaa aaacgaagca atgctcatca cctatgcaga
    16441 tagtttagga aaaaacatgg gcgaattaaa acaagtctta gacaaacatt tacaaggtgt
    16501 tattggtggt gtacatctgt tgcctttttt tccatcgaca ggtgatcgag gctttgcgcc
    16561 gagtgattat acgacagttg atccagctct tggaacatgg gaagatgtgg aagcattagg
    16621 tgaagattat tatttgatgt ttgattttat gattaatcac atttctcgtg aatcaaaatt
    16681 tttccaagat ttcaaaaaaa accatgagca gtcaccatac aaagaaatgt ttatccgtat
    16741 tcatgaattt ttcccagaaa atcgaccaac acaagaagat attgatttaa tctacaagcg
    16801 taaagacaaa gcgccgtttc aaacggtaga atttgcggat ggtacgacag aagaggtttg
    16861 gaataccttt ggggaagaac aaattgattt agatgtgacc aaagaagttg tgaaagaatt
    16921 tattcgagaa accatcaaag atatggcgaa ccatggctgt tctttaattc gattagatgc
    16981 gtttgcttat gcgattaaaa aattagatac caatgatttc ttcgtggaac cagatatttg
    17041 ggaactttta gacgaagtgc gtcaagaagc agcgaaatat gacgtcgaat tattaccaga
    17101 aatccatgag cattattcca ttcaaatgaa aattgccaat catgattatt atatctatga
    17161 cttcgccttg ccaatggtca ctctgtattc actttatagt ggaaaatcag aacgtttagc
    17221 caattggttg aaaatgagtc cgatgaagca gtttacaaca ttggatacgc atgatggaat
    17281 tggtgttgtg gacgctcgtg atttattaac agatgaagaa ttggattaca cctctgagga
    17341 attgtataaa gtcggggcga atgtgaagaa ggtctattca agtgccaatt acaataattt
    17401 agatatttat caaattaaca gcacctatta tagtgcatta ggcgataatg accgtagcta
    17461 tctgttagca cgtgcgattc aagtatttgc gccagggatt ccacaaattt attacgttgg
    17521 gttattagca ggaaaaaatg atattgaact gttggaagca acgaaagaag ggcgtaatat
    17581 taatcgtcat tactatgatt tagccgaaat tgaagaagaa gtgcaacggc cagtgattca
    17641 acaattattt gcacttttaa aatttagaaa tgaatctgcg gcgtttgatt tggatggaac
    17701 gattgaagta gcaacgccat cagaatcaga aatagtgatt acacgtaaaa ataaaaacgg
    17761 tagtgatagt gcaacattaa cggctaactt ggatacgaaa gaatttatca ttgagtctaa
    17821 agatataaaa attttcacta gtgttgcatt aaaattttaa cctattccat agaacaaaaa
    17881 atcctttata ctagcacttg gatgacttat ccaagaccaa taaaaaggac tcatacatgg
    17941 ataagtataa aacaaataca gcatttaata aatggttttc tgccataaaa ttaggaaaac
    18001 tttctgtcaa ctcccaaaaa tgtatttttg attacaattc gtatcgtaaa aaactgtctt
    18061 ttgaagctgt gctcaagctc tttctctacg cagttaatga agaaaaagaa agtttgcgtg
    18121 atttgagtac agctcttatc gaccgatcgc tccaactaga aaccgatgtg tcatcgatta
    18181 gtcataccca attgtctcga gcatttaatg cacttgaacc taaagtgtta gaagaaattt
    18241 ttcaacaact gttagaaaaa gttcaacatc aaatcaaacc aaccaagcga aatcatctgt
    18301 atctgattga ttccagtacg ttttcgctta gtttaaaacg tcataagtgg gcgactttcc
    18361 gtcaaacaaa atcaggtgtc aagttacact taaactattg ttatatggac gatactacga
    18421 tgtatccaac tgattttacc ttaacaaatg cccatgaaca tgatctcaat caactaccag
    18481 tattggtgaa tcaaccagag gcaacttacg tgtttgatcg tgggtattag gactttgaaa
    18541 agatggatca gatgcattgg gatggctatt tcttcgtcac tcggattaaa aaaaacacca
    18601 aagttcacgt gatagatact ctggaaacat cgccagaaac agaaattcta cgagacgagc
    18661 tggttcgttt aggatctaaa acctatctaa ccgccaattt ccgactagtc actgttcaag
    18721 ataagaacgg acgtgtgttt caatttatca ccaatcgaat ggacgtctct tcaaaagaaa
    18781 tctctgatat gtatcacgct cgttggcaaa tcgaattgtt tttcaagcac atcaaacaac
    18841 acatgaccat taaaaccttc ttttctcaaa gtgaaaaagg cgtgcagaat caattgattt
    18901 taacgatgat ttccgctctc ctcacgtttc tcatcaaatt ggagacgaaa acggaaaaat
    18961 cagtgttcca aatcaaacga ttctttcgtt atttactgtt tcagcccttt gaatgttgtc
    19021 tagaaaaatt aattcccaca tgagaaaaaa gaaatttggc tcgttacttt tggttttcag
    19081 ttgaataaca gataaaaaat tttctggaaa aagtcatcct ctgcgtcagc acagtttttt
    19141 ttgacttatt ttcagaggaa atagttttca gaatattcta aaatgtttta tgcaacacta
    19201 gtgatatttt ttagaattag atttctaatg tgcatttttt gagtattaag gagcaagtat
    19261 acaaagattt ataaagctga ttaaatttga ataaaaatat gcaggaacgc tactattttt
    19321 agtagtgttc ttttttatat ttgaataaaa aatgtggtgg ttaaatggtg gttataacaa
    19381 aaaatgaatt aatgaaatta ggttatggga agtatcagtc tgagagaatc atacgacagg
    19441 caaaagcaat tatggcttca aaaagctata cctattacga aaatagaaga ttaggactag
    19501 tacctgttga atctgtagaa gaagttttag gtatgaagat agaggtagag gaggattttc
    19561 agaatcatgg ttaattttaa aaaagataaa aatgggaaat atcattttac tgctagtttg
    19621 ggattcgaca aacgaacagg gaaaagagta caaaaaaacg tagaggggtt aaaacagtta
    19681 aagaagaaaa aaagcgtata ccgaacttgt ttctaattat ggaaaagaag cagaaacttc
    19741 tcaaagtact atgttgtttg aggagttttt ttataaatta ttcctacctt attatcaagg
    19801 gaaagtagaa gaaagaacat ataaaaatcg gttatctaca atcaaaaaaa cttttcttat
    19861 ttctttaaaa tggagttaag agaaatacgt ccaattgaca ttcaaaagtg gcaaaataat
    19921 ttattgagca aatataaaaa ttcttatgta agaaacatgt atgggatttt tcagatgtca
    19981 ttagatcgag cagttgtttt gggactactt gaaaaaaatc ctgcaaaagt tattggaaat
    20041 gtgaagaaaa ggaaaactac agttgatttt tggacaaaag aagaatttac aaaagttatt
    20101 tcaacttttt atattgatga tttgtaccaa cattttggtt ttatttgtat gtggttactt
    20161 tttatgaccg ttatgcgttt gggagaagcc actgcattaa cttggaaaga tattgatttt
    20221 gaaaataaaa acttgcgtgt gaataaaaca ctctattata agagagccaa aacctatgag
    20281 tttgtagaac ccgaaacggc ttctagtatt cgtacaatcg aactagacga caatacaatt
    20341 acctttttaa aagcttggaa ggagcgtcaa gtttcaatag gaggcatgtc gtttgtactt
    20401 tcttataatg gaacacctac tcagaaatat acaatccgtc atattatgag aagacatgca
    20461 acgatcgttg gtgttcatat aatccgcata cacggattaa ggcatagtca tgcttctttg
    20521 cttatatcac aataaaaaga tgcacttcga attaaagaaa ggttaaaaca tgctgatgta
    20581 caaacgactt taggaactta tggtcatttg tatctaaatt cattgagaga gataacaaat
    20641 gatcccacag attatatcca aactaatttt tctaaaatca aaccgacacc tatttacgat
    20701 aataaaaaat aaatgataaa caaaagtagg atcacaccaa aagaaacaca ctagaaaaac
    20761 tataatacag gcttttctag cgtgttgttt cttatttccg cctatttata cattatactc
    20821 ataaagcatt taatgcatcg atcgtagccg cattgtctat atgggtatat aaagttgttg
    20881 tattggtact tgaatgtccc aattgttggg cagttaatac caatgaattt gtctgttggt
    20941 agagtctagt agctaatgta tgacgcagtt tgtgagggct gacttgaatt ctaaagcctt
    21001 ttgaatattt tgctaccatc cgttcgattg caataccgga tatacgcttg gcttctcctt
    21061 ggtaaattgc tagaaataaa gctttttcgt tttctgttac tttatatcgt tctttccgaa
    21121 tagctagata attagctaaa tattccattg cgaatgctgc tatattgaca gcatctcttt
    21181 tatttccttt tctagttact accacattat tcttatctaa agataggtca tctaaattaa
    21241 tatttataac ctcggagagt ctcaatccag atcctaaaat cagtgcattg atcgccaggt
    21301 ctctttctac atctcgttga taatagatca aagctcgttt actcaaagta tttttatatt
    21361 cattggcaat ataatctaga taattttgtg tatccttatc tagaaataat tttttctcaa
    21421 tggcattggc acgtgcagct aacgtagttt tatctgtgat ggttgctact tgatccatag
    21481 gattattgtc taagtatggt tttcctagag gattattgga acttttcgaa agatatttaa
    21541 atagaacctt taatgccgtg atatttctat tgatagtaga agtagataga cctaagttat
    21601 tattatcttt gagcttagtt tttgttgtct ctttttttac attttctttt gtgcgacgtt
    21661 tcaaatgaga tttgtacaag tttaggtcat aggcagttaa ctgtcttatc gaggtgatag
    21721 aagtatcagg aacgatagac tcactcagaa gccattcata aaagagctta taatttttta
    21781 gatattctaa agtggttcga ggtgattgat ctttatcctc acgatcttga acaaaatctt
    21841 ttatataatc gggcatatga agtatgattg tatctatttt ttgatatatt tctgtatttt
    21901 tcatgtcatt catcctttca ataagtaggg gagaagagta tctctataat tggactatac
    21961 caattataga atgttttgtc aattggtatg ctctaaaatt tattaatttg tttcaaaaaa
    22021 agcatataaa caggtatacg tttaataatt taaaataaat ggtatacata aatagggaag
    22081 gaaatagaag ggagcaatct aaattttttg aattttctta attaattata tagaaagaac
    22141 aatctttcta tagagttaga catgagaggg actatgcttg atttaaaatc aacaagcgta
    22201 tatactgtat atacaaagaa aaaggaggga gaagactatg gcaattaaag aaagaaaatt
    22261 tagggaagtg gggaactcag tagtattaac tatttcaaag gagtttctta aagaaaatgg
    22321 gttaaatccc ggagatacgg tttttttaga tgaagaaaaa cttcgagcgg caattacaaa
    22381 aaaacaagcc ttatctgatg aaattgattt agcaatatct caagcattaa ctgaatatga
    22441 tactactttt agagagcttg tcgataaatg agtattcatt acctagaagc caaagatatt
    22501 ataaaaatga atgtaatcca aataaaaaaa tactctccta atgaacccct tggtgtaaag
    22561 gatgcgaatg ctttagaaat gtgtgtaggg caacttcgag cctcagcttt tggtgaagag
    22621 gtttatcctt cgatttatga aaaagcagct atcctactta tacaattgat aaagaaacat
    22681 ccttttcaca atgcaaataa acgtactgct ttcttagcta cttttgtctt tttgaaaatc
    22741 aatggacagc ttttagctat tgatcaaaaa gatgctgtaa atttagcggt atatattgct
    22801 acgtatgata aggatttcga tcaactaaaa tatgaagtga tcaacactat aaagacacat
    22861 acgcagccca tttaaggagt tactgtatgt gtctttatag ggttattagc tgctatagcg
    22921 aaataattga agtaaattac aaatagctat aattcaaatc caactatata gaaaacgtaa
    22981 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
    23041 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
    23101 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
    23161 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
    23221 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
    23281 cttatgaaat gaaagacctt ttacatcttg cagaaatcgt tactgggatt aaactaagca
    23341 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
    23401 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
    23461 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
    23521 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
    23581 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
    23641 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
    23701 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
    23761 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
    23821 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
    23881 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
    23941 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
    24001 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
    24061 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
    24121 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
    24181 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
    24241 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
    24301 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
    24361 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
    24421 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
    24481 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
    24541 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
    24601 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
    24661 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
    24721 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
    24781 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
    24841 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
    24901 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
    24961 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
    25021 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
    25081 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
    25141 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
    25201 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
    25261 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
    25321 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
    25381 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
    25441 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
    25501 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
    25561 ccccacttag c
//
LOCUS       DASJSZ010000035        25556 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.035, whole genome shotgun sequence.
ACCESSION   DASJSZ010000035 DASJSZ010000000
VERSION     DASJSZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.035"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..2232
                     /locus_tag="U4B03_001356"
     CDS             172..2232
                     /locus_tag="U4B03_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEN2068215.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2315..2884
                     /locus_tag="U4B03_001357"
     CDS             2315..2884
                     /locus_tag="U4B03_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2068216.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3059..3865)
                     /locus_tag="U4B03_001358"
     CDS             complement(3059..3865)
                     /locus_tag="U4B03_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068217.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4003..5517
                     /locus_tag="U4B03_001359"
     CDS             4003..5517
                     /locus_tag="U4B03_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEN2068218.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5920..6903)
                     /locus_tag="U4B03_001360"
     CDS             complement(5920..6903)
                     /locus_tag="U4B03_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEN2068219.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6908..7555)
                     /locus_tag="U4B03_001361"
     CDS             complement(6908..7555)
                     /locus_tag="U4B03_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2068220.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7640..8272)
                     /locus_tag="U4B03_001362"
     CDS             complement(7640..8272)
                     /locus_tag="U4B03_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2068221.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8288..8839)
                     /gene="coaC"
                     /locus_tag="U4B03_001363"
     CDS             complement(8288..8839)
                     /gene="coaC"
                     /locus_tag="U4B03_001363"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEN2068222.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8836..9573)
                     /locus_tag="U4B03_001364"
     CDS             complement(8836..9573)
                     /locus_tag="U4B03_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEN2068223.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9570..10559)
                     /locus_tag="U4B03_001365"
     CDS             complement(9570..10559)
                     /locus_tag="U4B03_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN2068224.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10817..11359
                     /locus_tag="U4B03_001366"
     CDS             10817..11359
                     /locus_tag="U4B03_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2068225.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11376..12461
                     /locus_tag="U4B03_001367"
     CDS             11376..12461
                     /locus_tag="U4B03_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068226.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12473..13279
                     /locus_tag="U4B03_001368"
     CDS             12473..13279
                     /locus_tag="U4B03_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068227.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13280..14107
                     /locus_tag="U4B03_001369"
     CDS             13280..14107
                     /locus_tag="U4B03_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068228.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14104..15177
                     /locus_tag="U4B03_001370"
     CDS             14104..15177
                     /locus_tag="U4B03_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2068229.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15605..18301
                     /locus_tag="U4B03_001371"
     CDS             15605..18301
                     /locus_tag="U4B03_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2068230.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKSIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18378..18866
                     /locus_tag="U4B03_001372"
     CDS             18378..18866
                     /locus_tag="U4B03_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2068231.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18878..19372
                     /locus_tag="U4B03_001373"
     CDS             18878..19372
                     /locus_tag="U4B03_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2068232.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19393..20202
                     /locus_tag="U4B03_001374"
     CDS             19393..20202
                     /locus_tag="U4B03_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2068233.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20199..21020
                     /locus_tag="U4B03_001375"
     CDS             20199..21020
                     /locus_tag="U4B03_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2068234.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21071..23170
                     /locus_tag="U4B03_001376"
     CDS             21071..23170
                     /locus_tag="U4B03_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEN2068235.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23167..24285
                     /locus_tag="U4B03_001377"
     CDS             23167..24285
                     /locus_tag="U4B03_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN2068236.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24324..25202
                     /locus_tag="U4B03_001378"
     CDS             24324..25202
                     /locus_tag="U4B03_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2068237.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25393..25545
                     /locus_tag="U4B03_001379"
     CDS             25393..25545
                     /locus_tag="U4B03_001379"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN2068238.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8074 a         4451 c         5409 g         7622 t
ORIGIN      
        1 acaaaattaa aaagtacata agttattatt ccgattttct tgaaacaata aaaaatcaaa
       61 aaaaatactc tcctaatagt ccatcttctt ttgattttat ttatataatg aaatcatctt
      121 atgaaaatta ctacttcatg tttagacaag agtagggagg actaagaata aatgtgttca
      181 aaaagacgtt caaaacaact aaataaacac gcttgtcgtt tagataagga acggtcgtgt
      241 tctgaaaaac actacgttaa gtttatgatg cgcacgatgc tgatcagttt atctgtaggt
      301 gtctatatgc atgtacccgt tcaggcaacg acacaaacca tcaaggtcgt acgtacctct
      361 gtttctgaga ccactgattc ccctatcact attactcata ctttcactga tgcaaatgga
      421 aaacagctac ttcctgcatt gacttcctat aataaagtca gttatcctcc aacgataaac
      481 cactactggt taattcctaa caaaacatta tggacatcgg aaaccggaag cagtgtgccg
      541 ctttctatct tttccgatat gactgatcct acaaaaggtg atcagttaaa acagatcact
      601 gatattttga atcatgcttt gacagatata aaggacggtg gaagcgcaca agtttcttat
      661 gtatatgaaa aagattattc acagtttgaa agaaagaaaa tcgatgttct ggcagggaca
      721 aaagtttcga tatctgatct ggtagaaagg ctgaagaacg cagatggaac acaaggagat
      781 atgtccaaag taatttcaaa caatgggaat atcgtggata cgactaatac aggaacatct
      841 actatttcct tgtcctattt tgatccaatt gctttaaaaa caatgactgc atctgctgtg
      901 atccatgttc gtgctgatca aacagctatc aaaggaaaag atcttcactt aagaacagga
      961 gagagttacg cccctaaaga tcttgttgaa tatgtaacaa aaagcgatgg aacttcagga
     1021 aataaagaag aaatcaaaat gaacgggaca gtagatacta caactccggg agaatatccc
     1081 gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg cagaaacaac agctgtagtt
     1141 tatgtagaag catctgctgc ttcatcgact gtcatcactc attttataga tagtcaaaca
     1201 aataaagaac ttcaagcaga aacacatatg agtagtgtaa atcgtattcc tgatttgcgc
     1261 ctacctgctg aattttctga tcgagtgcca aatatagatt tatcgactta tgaacaaaat
     1321 gggaaagtca cttctatttc tgattggatg caagcaaacg gattagacag aaaaagtcac
     1381 tggtcggaga tccttgaagt acttcactca gaactgatga ataataggaa gcaatcagga
     1441 ggggaaatta cctttactta tgtttatata ccagacaatc gttcattaga aggtcatgat
     1501 cttattgttc atccaaacga aaaaataaac aaagaagagt tgatccaatc agcaacaaat
     1561 tccttaggta aacccgtacc gttaaaggat gtatcggtta caatcaatga agtaccattg
     1621 gaggatggtt atttgaccaa acaaacaggt gattttacga ttatgtatag ttatcatgac
     1681 ccgtattcac tccaagaact ccatgctcag tcactattac atgttttacc attaaacaaa
     1741 cagatagatc aaaaagaaca aaaaagacaa gaagaaagct tgtcagaaaa aaaacaacaa
     1801 atggctcatg cagtttctcc aacagggaaa gtaaaacaaa caagaagcct agagttagca
     1861 agaatatcta gaaaaatctc agaatctaat aaaacgataa ggcaagaagc tcatttggcc
     1921 ccacaagttt ctggattgga aaatgaaatt ttagagccta aaataaaaca acaatcagaa
     1981 aaagaatttg ttgctaaaca gtcggaagat cagaaggaat cagatgacca gaaggaaacg
     2041 aagaaaaaac gcttgtcaac tattgattcc accgatgagc atctgcagcc gcaagtacca
     2101 cttcctagtc cgggaacacc cgcacctggt ggaggtattc cttcttctgc tttaggagat
     2161 ttcatgcgag gtatggcagg aacatgggta tacgctaatc gaaatgaatt tgacgcagat
     2221 gactggttct gatttatatc tctatctgtt atagtcaaat aagtagctga gtcatatgtt
     2281 atttggcata atggagacga ggagggatag aaaaatgtta gataaaacgt taccttacgc
     2341 agaaatctgg atgacaagac cacttcaaca aaatgttcca atcacgcctt tagcggaagg
     2401 ttttgaattt cgattttatc aaaaagaaga tgaatctgct tgggcggaga ttgagacttc
     2461 cgttggggaa tttcaaacga cagatgaagc acttgagtat tttcaacaaa actttgctcc
     2521 ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag acaaaaaaag gagaaaaagt
     2581 agcaacctgt accgcttggc gaaaaaaatc aagagagcaa gtgtatccgg tttttcattg
     2641 gttagcagtg aaatcagatt accaaggaaa agggctcgca aaagcattga ctgccagagt
     2701 cttgcaagaa tttcctaccc tccatacgga cggaccaatc tatcttcaca cacagacatg
     2761 gagccatcag gcgattgctc tttataaaaa aatgggtttt acttttattg ctgaaaatct
     2821 agacggcaca cctaatccag attatgaaaa agttatgcag gttttaagta aattagataa
     2881 ataacgaaat ggtgaaataa gtcctcagta ttgggataaa aaacgaaata gtcattcctc
     2941 atatcttact tttttgagtt aatactatcg gataactaat ggcatgccat acattcaaaa
     3001 aagaaggggc agtgacaaaa gtattgtcac tgccccttct cttgtttttt agcatgtttt
     3061 atcgtttgat ttttttagga ataaaattct tgatgaacgt aaatttttgg taatcgtttc
     3121 gactatagag gtaaccaaat acactgaaaa caactgctcc aataaaatta acaaataaat
     3181 ccttcatagt atccatgatt cccacgtcta agtaaccatt ttggatgatt gttttatctg
     3241 ttttaccatc tgcattttga ctttcaatga ttgtccgagt gatatctgga acatgataga
     3301 cagtgtttcc gtctttactt aactccacac tattgatttg ctggatgatc gtatcttttt
     3361 gcatatcgaa ttgcagtaat ttatctgctg aaaattccac aaactcccac ataacaccaa
     3421 ctgtcatcga aaaacagaag gaaacaactg ctagtaataa aggagtcaag ttcatggttt
     3481 ttgtattttt attgagcaaa taaacaaggg aaaagccgac acccccagct aaaaatccgc
     3541 tgaatgcatg tagaaaagta tcccacattg aaaaatagcc ataaaaatca cttaactctc
     3601 ctaaaactgt tgaactaaaa ataaaagata acaaaatgat ttctagcata tttggaatct
     3661 gtatctttaa tatcttttcc agaaaataag ggatttcaaa taaaattaat gtcagtaata
     3721 aaagcattgt atttccccag tgttggttcc agacctgatc cactagcata ataacgacga
     3781 tgatacgtaa taaaatatag attccgtttg tgattctacc tgcagttttt gtactgttcg
     3841 taaaaaaggt tttcaaatga ttcataaaat aactccttca tgtatatcct taattatact
     3901 aagaagcgtg tgtttaccaa agctatatga cttaaagaaa aacagagttg atattgtgaa
     3961 aaaaatcact taatatagta taaggagaaa agggggatac aaatgaaaaa attagttgct
     4021 ggtttattaa cagccgttgc acttcttgct atgagcgcat gcggcgcaga tcagaaaaat
     4081 acagcgcaga caagttctag tacgaaggaa tctgccgaag tatcatcagg cgcttctgaa
     4141 caagtatata cagatccaaa agaaatgaaa gaatcttatg atgtcgtgat tattggagct
     4201 ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg ctgggttgaa tccagtaatt
     4261 ttggaaaaga tgcctgttgc cggaggaaac acatctaaat catcaagcgg tatgaatgct
     4321 tcagaaacaa aatttcaaaa agaagaagga atcaaagact cgaatgatgc cttctatgaa
     4381 gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa tgcttcgata ctttgtcgat
     4441 cactctgctg atgcggtcga ttggctagat caaaacggta tcaaactgga caacttaacg
     4501 atcactggag ggatgagtga aaagaggaca catcgtccgt ctgatggctc ggctattggt
     4561 ggatacttag tggaggggtt attgaataac gtccatgagc gggaaatccc tatttttgtc
     4621 aacgcagatg tgacagacat taagaaaaat ggggaaacgg tcaatgaagt gactgtacat
     4681 gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa tcgtagttac cacaggcggt
     4741 tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg atttagctga ttatattacg
     4801 acaaatcaag aaggatcaac tggagatggt atcaccatga ttgaaaaatt aggtggacaa
     4861 accgttgata tggataaaat acaaattcat ccaactgtgc agcaggatga gggggtcttg
     4921 attggtgagg ttgttcgcgg agaaggagca atcttggtcg atcaagaggg gaaaagattt
     4981 gtcaatgaaa tggatacaag ggataaagta tctgcagcaa ttacagcatt gcctgaaaaa
     5041 tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg ccacagctat tgaattttat
     5101 gaccaaaaag gatatgtcac aactggaaag acaatggaag aattagcaga aaaaatcgat
     5161 ttaccaaaag aaacactagc aaaaacagtt gatggatgga atacggatgt tgcggagaaa
     5221 aaagatgctc aattcaaccg tgccacagcc atggaacatg acttatcgtc tccgaattat
     5281 tatgcgatca aaatcgcacc aggaatccat tacacgatgg gcggtgtgaa aatcaatacg
     5341 aacactgaag tattagataa agaaaaccag ccaattaggg ggttatatgc tgctggtgaa
     5401 gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa attcagttgc ggagatcatt
     5461 atttttgggc gacaagcggg catacaagca gctcgatttg cttcggaaaa tgaatgaaat
     5521 agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga gctaataccg agggtcagct
     5581 gattgaacac tgtttagtga agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa
     5641 cagccaagta aaaacagctt cttatgtttt taacttggtt ggacttatta ccgcagatgc
     5701 aagcttttga acaccgttta ttcggaaaaa gagcgtggga caaaagtaaa gaacactttt
     5761 gtctcacgct ctaaacacgt ataaacggcg gaagcagaag aaattccttc ggaaataagc
     5821 tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta
     5881 aacgcttctg tcccagcctc ttttttgatc agcatgattt tattcagtta tcttcaaaat
     5941 gatttttccg atgtttttgt ttgcttccat atattcatgc gcttgtttga ctgcgctgaa
     6001 ttggaatgta cggtcaatga ttggccgaat cttgccattt tctagaaaag gcccagcttt
     6061 ttgcataaaa gtttgtgcga gctctgcttt atactgatca cttctaggtg tcaataatgt
     6121 accaagcagt tggacacgtt tcccaatcaa tacaccaagg tctatctcag gcacgatggt
     6181 cccacctagc ataccaatca agacccaacg tccatcaacg gctatagaag ctaaattctt
     6241 ttcccagtaa gaagcgccga tgaaatccaa aataacatcg actccccgac cttcagtgat
     6301 ttttcgtacc tcttcttgaa agtcttgttt tttatagttg atcaattcat ctgcaccaag
     6361 ttctttacaa aaggcgagtt tttcttctga tcctgcagta acgatgattt tcgcgtgaga
     6421 aagctgtcta gctaattgga tcgctgctgt tcccacacca cttgctcctg catggatcaa
     6481 gacagtttcg ccttcttgta attttccaag ccagtaaagt gtttgataag cagtcaaaaa
     6541 aacttctgag attccagcag cagaaacata gtctagcgta tcagggagaa ggattgcacg
     6601 atcggcaggc atcacagcat attcggcata accacctaga ttgactaatc cgtatacttt
     6661 tgtcccagga gtaaaagtgg agatatcact tttattctca actacctctc cagctacttc
     6721 gactcccaaa atagggtagg gaggtttcaa agagactgat tctcttttca taatatctgt
     6781 tcgattgaca gctgctgtat ggactttgat cagtaattcg ccttgttttg gtttaggaac
     6841 aggcacttca atttgttcta atacatctac agaaccagga tgcttgattg aaatggcacg
     6901 catggatcta ttcctccttt gcccattctt ggaatgctgt attcaactct ttgctacctt
     6961 cagaaagtac tagagtcgta cgagaaatat ccgtttcttc cactagagcg gcaagtacag
     7021 cagcaactgt agagcgagtg atcgtttttg atgccttctc attaggctta gttaattgaa
     7081 tggaagtaac ttcttcatcg ttagtcaatg tgactggttg aagaatgaca aaatctaagt
     7141 tagttcgttt cagccattca tctgcataat gtttagtaat ataataatct gtcatggact
     7201 caggccattt tgctggttca tctgcataaa cagcactgac catcagatag cgagagacat
     7261 gcgcattttc agctgcgatc actgttttga ttgcgccatc caagtctact tgcagaagat
     7321 tctttccttg agagccggca gcaaaaatca atacatcgat gcctttgaat gcttcggtca
     7381 ttttttccac ggaccaagtt aaatcaaaag agacatacga aacattctct ttttttacta
     7441 atgtttggga agcaacatct ctcacccctg caaaaatttc atgggaactt ggagctaaat
     7501 cttttatcag gtgtcggcca atttgtccgt ttgcacctac aacaaaaatt ttcatttgga
     7561 tcatcctttc gacgtcaaat ctttccattc aatagtaaca ccaaaagaga aaaaaatcag
     7621 agatcggcta cgatgactat cagttagtaa gagaagggtg cttgaagtat cttaacaaga
     7681 cattgcatac tgctgcagtc gcaagacctg cgacgattcc ttcaggtata ccacttgtaa
     7741 aaataaccgc tagaatagct gaataaacag catttaaatt tgttccgatg actttggcat
     7801 aatcttgctg gaataaaaag tagatcatat tcatcactaa aatcgtattg gtcaatgaac
     7861 cggaaagtcc agctaagaat aaagaaacgg aatctgcttt ttgtttcatt agtttgcgca
     7921 gacctttata aacgaaataa ggaaccaccc caatcaagat gcgaggaata aacgcgatca
     7981 gcaaggcttt ccagcttcca tgttctgttc cgatgactgg gataaacgga gaaaagacaa
     8041 aagacaaagg tgtttggata actgtgctac ggatcatgct gatcagtcca aatgtaccgc
     8101 caagaaaagc acctagtctt ggtcctaaaa cgatcgaagc aatgattaca ggaatatgca
     8161 tggttgttgc attgattgga ccgataggga taaaacctaa aggcgtaaca gctaataaaa
     8221 tcaaaatagc taaaaacatc gcagttaatg taaagttttt tgtatttttc ataagtactc
     8281 cttttttcta agcatttctt tcattaagag tcagcatgac ctgttcgatg atcgtctgaa
     8341 tatcagctaa ggcacctttt ccaaaatctc cacaagctaa aagagattct cttggttcaa
     8401 tttcctgata gccgacagat ctcaatgttt ccagattacg ctgagtgatc gggttttgat
     8461 acatattcgt gttcatggca ggggcaatca attttggtgt tcccactgga agcgccattg
     8521 cgactgttga aagaagatcg tctgcaatgc cgtttgctag cttgccgata atgtttgctg
     8581 tagcaggcgc gatgagaaac aagtcagctt gttttgctag ttcgatatga ttgattacct
     8641 gcggatttga ttcttgcatg acgtctgtat ggactgcgcg cttcgacaga gattgcaagg
     8701 ttaatggcgt gataaaatgt gtgctgcttt tagacataat cacatctacc tgggcaccta
     8761 atttgacgaa ttgacttgta agatcagcgc ttttgtaagc agaaatactt ccagtaacgc
     8821 ctagtaagat ctttttcatt ttgattcctc cagtctaatt ttctcagtaa tcaatgcagc
     8881 aatctcagat ttactttgag cttcttccac tgttccatct ttagataaga gatagccgtg
     8941 gtgtgtctcg ccatggattt ccgtcaaatc atttgcgaaa ataaaatcag ttttgttatt
     9001 tatcagacta ttttttgcga ctgccagaag agattccttt ggtacatcta ctaatagttt
     9061 gaaaccaatg atttttgctt ttggctgcca ttcacgtaga taagaaatga tttttggtgt
     9121 tttcttcaaa acgagcacaa gatgctcggt gttagaagag atctttgaaa cagtggtatc
     9181 tgtctgttca ttttccgcaa accaattgtc cagttcttct tgcgttgaag aagtgggtag
     9241 gttttttgcc agctgttctt ctgaaaaact aaaagcagga gtaaaatcac tgactgccat
     9301 actgtggata atcgcatcgt agtgttcttt tttcgttagt tcttctaatt gtaaaaataa
     9361 gtccagtgca gattcgatcg tataaatcgt tatattggag tgtcttttcg gtttcagtgc
     9421 ttgtttcgta gtcacgtaat cgattgtggt atccggattg gcagctaaat aatcagctaa
     9481 tgcttgccct aatcgtcctg tcgaatgatt tgtaatagca cgtacttgat cgattttttc
     9541 agtcgttcct ccagctgtga ttagaatctt catttatggt cacctacatt tactagtgtt
     9601 cttccttggt gtgttccagc aagtagttta gatgctgttt caggcaattg atccagagta
     9661 atctcttgga cgaccagttc atctgcaatc tgcaaatccg ccatctgttg ccataaagaa
     9721 agacgttgat ctattggtac attgacagaa tcaattccaa tcaactggat gtttcgcaga
     9781 ataaaaggca agacagtcgc gtttagtttg attcccccag cattgccaca taaagctact
     9841 gcaccgttat aagagatcaa tggaagaaga ctgcttagct gctcgccacc tactgtgtcg
     9901 atgacatagt cgatttgctg ttttcctaaa gcttttgttg tctctggcag aaattcatca
     9961 ggtcgtacga cttgcaaagc acctagactt tttagccatg tgacagactc ttcttttctg
    10021 gaaaaggcaa tgattgactg gaatcctagt ttatgtaaca gggctatcgc tgtactgcct
    10081 acaccgccag aagcaccagt aacgacaact tttttatcgg aggtcatacc ttgacggatt
    10141 aaagcgttca cacacaacat agcagtaaac cctgctgtac caaggatcat cgcttgacgc
    10201 agcgtcatat tttttggtag aggaactaac cagtctcctg gtactttttg atattggcta
    10261 taaccgcctg gatgggtgac gccaagccca aaaccagtag caagtacaag atcttctttt
    10321 gaaaaacgtg gattgttact ttggacgaca gttccggata aatcaatccc tggagtcatc
    10381 ggatatgttc gtatcacctg accagactcg ctcattgcca atgcatcttt ataattgaca
    10441 tcagaatagg caatttttac caagacttca ttttcagcca actcagtaaa tggcagatca
    10501 ctgattgctg tatggaaaac aggttccttt attacctgaa aagatcgaaa agcgttcatt
    10561 cgtgagacac tccttttaaa attttctttt atcataccat aaagcctact cttttattct
    10621 tgttcttccg taaatttaga caaaaattag gtgaaaaata aagaatcaaa caactagaat
    10681 ttattccaaa agataaagaa atattcagca aaaaaaattt tacaaagggt tgcctttttc
    10741 aataaatttc gtatactaat acgagtggta gtaaacagga agtttactat tagaaaactt
    10801 cgattcggag gaaattatgg aaattggtga gaagttaaga aatttgcgca tccaaaagaa
    10861 tttgacacaa gaagaactag gcgaacgcac agatttgtca aaagggtata tttcccagtt
    10921 ggaacgagat ttaagttcac catcaatgga aacattcttt tctattcttg aagtcttagg
    10981 tgtgactcca gaagaatttt ttcatcaaga agcagcgaat ctgcaagtgg tttattcaaa
    11041 agaagatcat acggtttatt tcgatgaaga aaacgggtat gaattggaat ggctcgtggc
    11101 tgattcgaat gaaaaagaaa tggagcctgt cttgctgact tttgaaggtg caggagaata
    11161 taagacgttt gaaccttcat tatctgaaac gtttattttt gtattagaag gggaattaga
    11221 gttaagctta ggagagacgg tctatgtggc aaaagcagga cagtcgatct attatcaagc
    11281 gacaaaacaa catcaattaa ggaatcatct caaaaaacga acaaaagtat tgattgttgc
    11341 aacgcaatca tatttgtaat atagggggta aaaacgtggg gaaaccaatc atttcatttg
    11401 atcaagtagt gaaacgctat gatgatgaaa cagtattaaa aaaagtaagt tttgagatcg
    11461 aacaaggaaa attttataca ctgcttggtc cttccggctg tggtaaaaca acgatcctac
    11521 gtattatagc tggattcacc gaagctacag aaggggacat ctattttgaa gggaaacggt
    11581 tgaatgattt gccagctaat aagcgacaag taaatacagt cttccaagat tatgcacttt
    11641 ttcctcatat gaatgttttt gataacgttg cttttggatt gaagatcaag aaaatgtcca
    11701 aatcagatat cgaaaagaag gtaaaagatg ctttgcgtat ggtccagctt cctggatacg
    11761 aaaaacggga gattagtgaa atgtctggtg gtcaaagaca gcgtgtagcg attgcacgag
    11821 caattgtaaa tgaaccgaaa gtattgctat tagatgaacc gttatcggcg cttgatttga
    11881 aactacgtac agatatgcag tatgaactgc gggatctgca acaacgatta ggaatcacgt
    11941 ttatttttgt tactcatgac caagaagaag cgttggcaat gagtgatgag atttttgtca
    12001 tgaataaagg gaaaatcgtc caaagtggta cacccgtaga tatttatgat gagccaatca
    12061 atcatttcgt tgctgatttt gttggggaga gcaatatcgt ggacggtgta atgatcgaag
    12121 ataatctagt tgagtttgtt ggtaagcaat ttgaatgtgt cgatggcggg atgcgtctaa
    12181 atgaaccagt tgaagttgtt ttacgcccag aggatttgac gatcacgaca ccagataaag
    12241 gaaaactagt cgtaacggtc gatactcagc tgtttcgtgg tgtccattat gaaatcatct
    12301 gttatgatga acagcaaaat gaatggatgg tccattcaac gaaaaaagct aaagaaggaa
    12361 gcaaagtagg attggccttt gagccagaag atatccatgt catgcgtttc aacgagtctg
    12421 aagaagaatt cgatgcacgt cttgacagct acgaagaata ggggggacaa gaatgaaaac
    12481 aatgcgcagg atctattccg tcccttatat tttgtggctc ggtttattcg tgatcgcgcc
    12541 tgtattaatg atcatttatc aatctttttt tgatatgaat gggcaattca cgctgaataa
    12601 ttatctatct tattttacgt cggggacatc gctttctatg acgatcaatt ctgtatggta
    12661 tgcatttttg atcacgtttt tcacgctgat catcagttac ccgactgctt attttttaac
    12721 aaaactgaag cacaagcaat tatggctgat gctgattatt ttgcctacat gggtgaatct
    12781 gctgcttaaa gcttacgcat ttattgggat tttcagtgtg cacggcagcg tgaatcattt
    12841 ctttgatttt atcgggatcg ggccgaaaca gatattattc acagacttca gttttctgtt
    12901 cgtggcaacg tatatcgaga ttccatttat gattatgcca attttcaatg cattggaaga
    12961 gatgaatcct tctttgatca gtgctagtag agatttaggc gcgaatagct ttgagacctt
    13021 ccgacgagtg atttttccgt tatcattaaa tggtgtgaaa agtggtgtcc aagcagtatt
    13081 tattccttct ctttctttat tcatgctgac tcgtctgatt ggcggaaatc gggtgatcac
    13141 tttaggaacg gctatcgaac agcatttctt ggttacacaa aactggggga tgggttctac
    13201 aatcggtgtg attttaatca ttgctatgtt catcgtgatg ctcctgacag gcgagaagaa
    13261 aaaaggaggt cgctcataaa tgaaaaaatt taaatggtcg tatctttatt tagggatcgt
    13321 ctttttattc ctatatgcac cgatcttcta tctgattttt tattccttca atgaaggagg
    13381 aacgatgaac agctttacag gatttacttg ggaaaattat gaagctgttt ttgaagatac
    13441 acgtttgatc attatcgtat tgaatacgtt catgctggct ttcttatctg cattgatcgc
    13501 cacgatcatc gggacatttg gggctatggg aatctattac acgaaaaaac gacgtgcacg
    13561 gacagcgttg ttgagcatga ataacatctt attagtctct cctgatgtta ttatcggtgc
    13621 tagtttcttg attttcttta cactactagg attcggatta ggttttcaga gtgtattgct
    13681 ttcccatatt gctttcagca ttccaatcgt cgtcttgatg gtcttgccaa aattgcagga
    13741 aatgaacgat tcaatggtcg atgcggctag agaccttggt gctaataatt tccaagtagt
    13801 caaaaatatc attttgcctt atctttctcc aggaatcata gctggatatt ttatggcatt
    13861 tacctattct cttgatgatt ttgcggttac atttttcgtc acaggaaatg gctttacgac
    13921 gctctcagta gagatctatt ctcgtgcaag acaaggaatc agtttagaaa tcaatgcgct
    13981 aagtgcgtta gttttcttgt tctccatgat attggtcatt ggatactact ttattagtaa
    14041 agaaaaccgt ccaaaacggc tgaagaaaaa cagaagagta cgcgaggggg tggcacggct
    14101 gcgatgaaaa aattacaatc tctgttttta ggtattctca tcattattgc ggtgctgctg
    14161 cttggtgttc gtcaacttga aaaaacaagt ggcatgagtg gagcaaaggt acttaccatc
    14221 tataactggg gagattatat cgatcctagt ttgatcacga aatttgaaaa agaatatgga
    14281 tacaaagtga attatgaaac atttgattct aatgaagcga tgttcacaaa aatccagcaa
    14341 ggtgggacaa actacgacat cgctatacca agtgaatata tgatccaaaa aatgatcaaa
    14401 gaaaaactag tacttccatt agatcattcg aagatcaaag gattaaataa tattgatcca
    14461 cgatttttag acttggattt tgatccagat aatcagtatt cgattccata tttttgggga
    14521 actttaggga tcgtatataa cgataaattc tttgatgcaa atcaaattaa gcattgggat
    14581 gatttgtgga agccggaatt aaaagacagt ttgatgctga ttgacggtgc acgagaagtt
    14641 atggggcttt ctttgaatag tttaggctat tctttgaata gtaagaatat ggcacagctg
    14701 acagaagcag caaataaatt gaatcattta acaccaaatg tcaaagcaat cgtggcagat
    14761 gagatcaaaa tgtatatgat caatgaagaa gcctccgtgg cagtgacatt ttctggcgaa
    14821 gctgccgata tgatgtggga aaatgaacat ttacattatg tgatcccacc agaaggatcc
    14881 aatctttggt ttgataatat cgttatgcca aaaaccgtga aaaataaaga aggtgcctat
    14941 gatttcatca actttatgct agaaccggaa aatgcggcac aaaatgcaga gtatattggt
    15001 tattctacac caaatgaaga agcaaaagcg ttattgccaa aagacatttc gtcggacgaa
    15061 caattctatc catctgatga cacgatctcc catcttgaag tctacaaaga tctaggatcg
    15121 aaatacctag gcatctataa tgacctattc ttagaattca agatgtatag gagataatgg
    15181 caaaactcag taagaatggg gacttatcaa cgttctaaag agctaaaaat cgtccttttt
    15241 ctatctgagt tttccaatct tctaccaaaa gcggatgaaa cgccgctttt tttgtgttat
    15301 tttatttttt ctaccacgtt tttctaccag agaaaagtgg acaaaaccat tgtaaaatag
    15361 ggcccgagac gggctagtaa atctaagatt gaaaaagtta cagaaggata aagtaattgg
    15421 aaactatgca aatatactta acccttctgt aatctattaa taaaactaat atatgaatcc
    15481 agttcaatat ttgtagctat gaaaaaacat aaaaatcata ttagataaaa actaaattgg
    15541 tagataaatt attttgtcag atatctgagg caaatattcg actatttgtg tttcaaagta
    15601 aaaattggaa tacaaatata gaatagacat attgcagccg atagtttatt atgcttttag
    15661 acacattaaa ctggaggaaa aaatgaaaaa gagcgctaaa gatcgagttt ttgatgagct
    15721 gatacgacta acagggaata tgtcttctgt cactgccgag aaaatttcga agaatctagg
    15781 tatctctcgt cagaatgcca gccattattt gacgagatta gtggaagata aaaaggtcga
    15841 gaaattaccg ggaaagcctg ttttgtggaa gcctttagat gaatatgcgg ttgtagataa
    15901 tacggagcaa ataaacgaag cctttcattc tgtagttggt catgacggaa gtctgagaga
    15961 ggtcattcaa aaatgtatag ccgctgtcaa atatccgcct aacggactca gtgtattaat
    16021 aaatggagcc acgggtgttg gaaaaagttt tttagccaca aaaatttttg aatacgctat
    16081 ccatgagcaa attattgaaa aagatgcacc atttgctatc ttaaactgtg ccgattatgc
    16141 ggacaatccc gaactgcttt cggcaacact gtttggatac aagaaggggg cctttacggg
    16201 ggctgaaaag gataccgatg gtttattggc aacggccaac aacggctatc tttttctcga
    16261 tgaggtccat cgcctatcga aagaaaatca agaaaagctc tttctattta ttgatactgg
    16321 aaactatcgt ccagtcggtg aaaatgtaaa ttggcattct gctaaggttc gttttatttt
    16381 cgccacgacg gaaaaaggtg aaaattattt attagacacc tttgatcggc gtattcaaat
    16441 tagtgttatg ttgccaactt ttgaagatcg ccccattcga gaacgattag agctgattca
    16501 attatttttt caaaatgaag cggatgtttt acaaaaagat attattgttt caagagaagc
    16561 cttgtcttta atgttatctc atccctttag tgggaatatt ggtaagctga agaatatcat
    16621 taaaatcagt tgtgcggatg tgtatagtcg aacacaagaa gaaccattaa cgattgggaa
    16681 aaatgagatc ttgattcagt taaatatgct ggaatccgaa gtagaaagct tgccgattgc
    16741 tagtttattg ataaagaaaa atcaaaagat aaaaacggat cgaatcaaac cgtttgatta
    16801 tgattcgatt ttggaaaaaa ttactcagct gataaatgaa ggtaatttca atgaaaattt
    16861 ttcttcgata aagaatgaag tacagcattt ggtgcatcac attgataagg attctttttt
    16921 atcaatgagt agaaaattga tgattcagat gtttagaaag gtctgggtat ccgttttagg
    16981 aaaaacgtac ggattgacga cgagtggacc tgttgcagaa attattagca gatcctatgt
    17041 catttcccca agctataaga cagacttaac cgggataaat cgtacattag ctgcaaatct
    17101 acctcggacg acttacttat gtaatcagtt cttggagcat ttaccaccgc tctcagaaaa
    17161 ggacgagaac tttctaagac tgcttattgt cgtgacttta tctgattatg tagatgaatc
    17221 catcgagctg aagggcctgt tgcttgctca cggagatagt acggctagca gcattcaagc
    17281 agtagtgaat caattatgcg gcaattatgt ttttgaagcg ttggatatgc cagtagagag
    17341 taatgtgtca gagattgtcg aatcgacaag gaatttcgtg aaacggcaag tgaatacaga
    17401 tagtctagtg ctgatcgtag atatgggctc tttaaatcaa atttacacac agatcaagaa
    17461 cgatttaaaa ggggagttac ttcttctgaa taatttgacg acttccattg cacttgatat
    17521 tggcttgaag atgacgatga atgcgccatt taaatcgatt gcggaggatg cagcagacaa
    17581 gtattcaatc aatgttcaat actttgaagg cttttctcaa agtagcaaca ttatcatttc
    17641 ctgtatgtcg ggtttgggga tttcggacaa attgaaagat gtttttcgtc atcatttatc
    17701 ggatgaatcc atcgaagttt tcacgaagga ctacagagaa ctacgtgaat taatcgacca
    17761 aaatgatgaa acttattttg acaagacaaa attggtcatc acgacttcgg atcttccacg
    17821 ttccttttca attcctaata ttaatgttta tgacatctta gacggtgatg gagcagccaa
    17881 tttggagcat attttgagtc ctgaaatgaa gccggcagaa tttaaccgct tgattcagga
    17941 gctagtaaaa ttcttttcgt tggaaggaat tgcggaccgt ttgaactttc tgaacccaac
    18001 gatcgtgatg agtgaagtgg aaacagtcat ttctaaatat gaaaattact atcatttcac
    18061 tttaaatggc cgtgtaaaac taaatctcta tatgcatacg gcattgatga tggaacgttt
    18121 attcttggct cggacagaaa aatctgaaga accgaattat gatttgaaac cagatgaaga
    18181 tgaattttat tatgtttcta gaagtatttt tcaatcaatg gagatgaaat ataattttaa
    18241 agtcaatgct tatgagcttt cgctgctcta tgaattatta ggaccgtata ttcaaaaata
    18301 aagtgtctaa aaaagtgtct aatctgtttt ttttaggtta gacacttttt ttaatgtgaa
    18361 ttaacgtgat taaaccaatg tttttaaaag ttggcacagg aattgcttat ataaaagtga
    18421 acagaaagaa aggaattaac catatgaaga aaattctaat agcgactcac ggtcacttag
    18481 cttcaggctt tcttagcagt attcaattac tgacggggaa ggttcaagag attacggtga
    18541 ttaatgctta tgtggatgag tgtgattttg aacaggagtt gaatgtattt gtaaaccagg
    18601 tgaacgagca ggatcagatt tttgttttta ccgatttatt tggcggaagt gtcaatcaga
    18661 agattaccaa aacctttttg gagaaagaga ttgctgtaac tattttgtct ggctttaatc
    18721 tgccaattct gctcgaaata gtattggcaa ccaaggaatt aacaaaggag gatattcagg
    18781 aactggtaaa taaatgccaa caggaattaa agatgaccat tcttgatgag gtacatgtag
    18841 taaatgaaga ggaagatttt ttttaggagg aagaaaaatg attaaagcag tgcgaattga
    18901 tgaacgatta attcatggac aagtagcgat gacttggaca aagagtctaa atttaaccgg
    18961 gttagtagta gcaagcgatg aggcggctac caacgatatt caaaagatga cattgaagat
    19021 ggctgcacca tcgaatgtca aagtcattat taaaacagtt gatgacgcga ttcatttgtt
    19081 aaaagatccg cgggcgaagg acatgcgctt gatggtttta gtccctaatg tcaaagatgc
    19141 ggtaaaagtt gcgaaagctt tcccaacgga aatcgaatta atgaacgtgg ggaatgctgg
    19201 gaaaatgaca ccgccatcgg aagataagaa aaccttatca aaggaagtga tgttaacgac
    19261 gtcagaaatt caagacttaa aagaattagt tgaactttat ccagataccg tttttcagcc
    19321 tacaccggcg atggaaaaac gttcagctat ctctattttg agtgattttt agaaaaagga
    19381 aggagaaaaa ttatgtatga tgcatttgta gtcgctttgg ccgtttttat tggagttgct
    19441 ggacatgaaa tgttcgggat gtccatgctg agtcgtccga ttgttgttgc tccgttagca
    19501 ggagcgttga tgggggattt gcagacgggg ttgattgtcg gggcgtcact agaagccatc
    19561 tttatgggtg tcgtcaatat cggaatctct agtacggcgg agccttcttt ggctgctggg
    19621 ctggctacag cgttcgcaat caaaatggga ggaaacgtgg atgcgattat tcccattgtc
    19681 tttccactag cagtattagg attacagtta atgaatatga ttttttcttt tgtatgtgga
    19741 ccgatggctg ctaaattttt caaatttgca aaatctggtg atcaaaaagg aatcatcaat
    19801 ctgcacttct ttatgtggtt tgtccactat gcgctttatg ctcttattcc attctttgcc
    19861 gttttatttg gagcagatgt tgtcaaaagc gtcttggata atattccaga ggtcatcatg
    19921 aacggattaa cagtcgctgg aaatctgctg ccggcagttg ggatggcgat gctgttacgg
    19981 atgctttgga gtaaaaatat tgctgtgtgg tttttctttg gggccgttgt tatggcgtat
    20041 ctcgagttac cattgattgc aattgccgca gttggaacga ttattgcaat tgttatagcc
    20101 caaagagatc tacaactaaa aaatatatct gttcatgcag ttcaaagcga tgctgctgta
    20161 aatgcaacgc ttgaccaaga agaggaggat ttctttcaat gaaattagct gatggaattt
    20221 ctaaagatga gaaaaaattg gttcggaaga tgttttggcg ctcgatgact atgtatgctt
    20281 cggtgaatcc ggtgacgatg ggcggcggtg gtttcgccta ttctatgatg cccttcatta
    20341 atcatttcta caaaagtgaa gaagatcgag ctgcggcgct ggaacgtcat acacagtatt
    20401 tttcgacgac cattccttgc gcgtcctttg tcatggggat tgcggcatcg atggagaagg
    20461 aaaattctga aaaagctggt tttgacgtgg aaagtatcaa cgcgatcaaa acgagtttga
    20521 tgggaccatt ggctggaatt ggcgattcat tgttctgggg agtttggcgt gtcgttaccg
    20581 cgggactagc gatcaactta gccaatgccg gaaatatttt ggcaccaatt atctttttag
    20641 ttatgttcaa tatacctaat tacttgatgc gatactatgg cggcgtttta ggctattcat
    20701 taggctctaa atatattgaa aagctgtatg cgaacgggtt aattgaaatt ctgacaaaag
    20761 ctgctagtat cttgggtctg atcatggtag gtgcaatgac gatcagtatg gtcaaattca
    20821 ataccgtttt gagcttttca atgaagggcg aagaactaat gaatgtccaa gaaatcctcg
    20881 atagtatttt taaaggaatt gtaccgttat tggtaacatt gagctgtttc aaattactag
    20941 ataaaaaagt cggcgtcatc aaaattattt ttggaatggt cgtcttggga attttattgt
    21001 cattacttgg aatcgcatag taggaagctg cagctactag gctgcagttt ttttagcaag
    21061 gagggaaaag atgactatta atgaagtaat ttattcaccc aaacacccat tttttaatgc
    21121 tgagttaaac gacggaaatg tgactacttc aaacgaatac agctactatc ggaatattcc
    21181 tgatgtaaag ggattgacct tcgatgtaag taatcaaaag acattagcaa accttgatgt
    21241 ttgcgggacg attcaaacgt tgacctttta tcgagaaaat ttactaactg aggaaaaacc
    21301 aggtgtttgg gtcaataaac agctctctca aaccaattcg ttatccttaa aggttgaagt
    21361 aaacgggatt gtctaccatt tgtcagaatc ggatcatcgt attgaagtcg atgtgattca
    21421 agactgtttg cctagatata ttcatcatta ttcgacgttt aaggtcattg tagtatcctt
    21481 tgctcccata ctggctaata agcgtttaag tatgcttgtt caacaggttt acattgtgaa
    21541 tgaaacacac gaaagccttc aagtttcagt gaaagatgtg ccttcgtatc aagagaagta
    21601 ttcggatcag caaaatgtgt tgatttctca aaaaggaaat aaggacacga tcgcacaaca
    21661 ggaagtggca agcttcgcca ttgcttttgt ggacccaaat gcttttgaag aagtaatgac
    21721 ttttcaggaa tctgatatcg aaaaatggct gaaggaaaca ttgaactatt ttgctcagct
    21781 atttgggcag ctgtcaatgc caaacgatag agttgtccat ctctatcgtc gtgcacttta
    21841 tcaaagcttt tctagtttcg gtatgaatcg acagggacag gtcgtcggca gcaattgggg
    21901 cagctatcct gctactaatc gaatttggaa taaggatatg tattacgcat cattgccatt
    21961 caccatgttt gaaccggagt tatgtcaaaa aacggttcta tggtttgatc aatatggcgt
    22021 taagtttcct ggttcaaaat ttacaggagg tatcaatcat tctctgagta attcgttatc
    22081 cagtgcgtta ctggctagtc tttattttga acatactggg gatacaaaat tctttgagcg
    22141 gtgtccgcag gttctactga atgcttcaag aattattgag gacatattag ctcaacgtcg
    22201 gtcaggggaa ccaatgcttt ttccatctgt ttggttatca gacgcctttg cacttggaaa
    22261 gtatcatacc ggcagcaatc tttgtctttg gaaggcctgt attggacttg cagaaatttt
    22321 tgaagcccta gaacaactga gtcttagcaa gcgatacaaa aacattgcct gtaaactaaa
    22381 ggaatcaatt tgtaaacaaa tgactatcga tggtcctttt ggtgagcaat ttttggaggg
    22441 aatcggtgat gaggagaaaa caacctattc agtgaagcac tatcaaaaac cgattcttga
    22501 gcaagggtta atctttttaa gtgatgtgat tgtggatgga aaaattaatt tattgatgca
    22561 tgatggagaa gaatcagaca ctacgttgat gcctttttat caatttttag ataataaaga
    22621 tcatttatat cagaacacca tggccttttc tgctagttct ttcaatccaa cctatagcga
    22681 ggaaatcaaa ggcatcactt ggggattaga atccggcgcc acttttcctg gctttatcac
    22741 tgttttaatg agcgagctgc acaaccctca agctttcgta acccgattag aagaactaat
    22801 ttgtttggca gatttagacg gttcgtggtg gtggtggccg tatcgcttag aggctcaaca
    22861 aggagaagtc gtgcgagact ttggctgcgg caaatgcggc tgggcatcgg gtctttttgt
    22921 cagcctaatg atcagtcaat atttcggatt gaaattcaaa aaaggagtat tgcaaattga
    22981 tcctgttgat aatttaacgt tttcttggaa gaatttgaag ttcggtcaag ccttcatttc
    23041 gattgaatgt acgcaatccg agttgagtat ttcaaatttg tcagaaaagc cattgaaaat
    23101 aagtgatacg aaaaaaattc ttcacgtaga aaagggaaaa acgattcaca ttcaaaggag
    23161 aaaaagatga gtatactaaa aaatattacc caatttaaaa aaatccagag tcgagcaatc
    23221 actcccgaaa atccaacagg cgagaaaggt aaagctgcga tgttgccaag cgagctagga
    23281 ccaagccgca aaggtcgtgg cgcaattccg ctgacttctg gtgaagaaac tgtgattgcg
    23341 gatattactg gaacgggaat aattcgtcat atgtggatga cgattcgtga tcgaacagaa
    23401 tttggcagtt ttgttttacg tgatttaatc ttaagaattt attgggatgg gtcggatagc
    23461 cctgctgttg aaagtccttt aggggatttc ttttgtaatg ggtttggaga gcgttgcgag
    23521 gtgaactcgt tgccgatagt ggttaatcct acagggggct ttaattccta tttcgaaatg
    23581 cctttccaaa catccgctaa aattacgatc gagaatttac atccgaagga tatgtcctca
    23641 tttttttata cgataaatta cgctttggtg gacgaattgg aagaaaatag cctatatttt
    23701 cacgcaaatt ggcatcggga acgcattacc aaattacagg aggattataa gttaattgat
    23761 cgattaaagg gtcaaggcta ttacgtgggt acttaccttg cgcttacctg cctagaacgt
    23821 tattggtggg gcgaaggcga gtttaaattc tacattgatg gggatagtga gtatccgacc
    23881 gtaacctcaa caggttcaga agattatttt ggcggggcgt gggcctttca tgagtacgat
    23941 gagttcggac gtccacatgc gaaaaactat tcaacagcct ttttagggta tccttaccaa
    24001 tcaaatagag atgccacaag agatcgtttt gaaacgggaa aattgaatgc cgttcatgcc
    24061 tttggtgacg acgcaatgcc acgacatggt ctttatcgtt ggcatcttca agaccccatc
    24121 tgttttaccg aagatttatc ggtcacttta caacaaatcg gcaacgatga tgttcgctta
    24181 tttgagcggc aagacgatgt tgctagtgtc gcttactggt attcaacaga agcttcagga
    24241 aatactaaac gtcatttcac tgatcgagaa cgcttgccgc gttaatgaag tgtttttcac
    24301 atattcgaag ggaggaacct atgatgatat atggggcaat tgaagctgga gggactaaat
    24361 ttgtctgtgc agtcggtact gaggaattaa cgattttgga aagagtcagt tttcccacaa
    24421 caactccagt tgaaacaatg aagcaggtca ttgatttttt taaacctttt aaggaacaat
    24481 tggcaggcat cggggtcggc tgctttggtc caattgatat tcaatgcaaa tcaacgagtt
    24541 tcggtcatat cacatcaaca ccaaaacttg cttggcaaaa tttcaatttt gtaggaacgc
    24601 taaagcaata tttcaatatt ccgattgaat ggacaacaga tgtcaatgcg gcttgctatg
    24661 gtgaatatgt tgctggttct ggcaaagggt tagcaggtgt tgcatacttc actgtgggaa
    24721 caggtatcgg aggtggtgcc ttgttcaatg ggactttcgt ggaagggttc agtcatccgg
    24781 aaatggggca tatgttgatc aagcgacatc caaaggacaa tttttcagga aactgtccct
    24841 tccaccaaga ttgtttagag gggatggcag caggaccagc gattgaaaaa cggttgggtg
    24901 taaaaggcca gaatttattg gctgacgatt cgttttggca gattgaagcc ttttaccttg
    24961 cccaatgtgc atacaataca acactgatgt tttcgccaga tagaattatt tttggcggtg
    25021 gtgtaatgaa acaagaacac atgaagaaaa aagttcagga taaatttgtt gagcttataa
    25081 atggatatgt tgaaatacca ccaattgata gctacattat tacgcccgaa ttaggtgata
    25141 atgccggaat aatcggcgga ttagcattgg ccagaaaagc ggtaagaaat aagcaaccat
    25201 aattaatgaa tgtttcattg atactcatgt caaaagtatt taaccgagta atcaaatgat
    25261 caaatgaagt tggataaagc gaaaatgaat tcaaataaaa aggctctaag tcaaatcgtg
    25321 ttgttttcat taaatcactg aaaggaaatg cttctgcgtt tccttttttc tttttgttgt
    25381 gcgctgtata ccttgaggat attcactggt ggcgtacact taaatagtaa tatattgtgt
    25441 tactttatgt ctgtggtaat agttatagtt gcttaccaat gtatgttcat ttttcgaaat
    25501 gttgacttca taatccttgg cttaatgttt gaattttttt ggtgatagat tttttc
//
LOCUS       DASJSZ010000036        24952 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.036, whole genome shotgun sequence.
ACCESSION   DASJSZ010000036 DASJSZ010000000
VERSION     DASJSZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.036"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(109..720)
                     /locus_tag="U4B03_001380"
     CDS             complement(109..720)
                     /locus_tag="U4B03_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2068239.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(843..968)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1050..1766)
                     /locus_tag="U4B03_001381"
     CDS             complement(1050..1766)
                     /locus_tag="U4B03_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN2068240.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1772..2341)
                     /gene="scpB"
                     /locus_tag="U4B03_001382"
     CDS             complement(1772..2341)
                     /gene="scpB"
                     /locus_tag="U4B03_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEN2068241.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2325..3122)
                     /locus_tag="U4B03_001383"
     CDS             complement(2325..3122)
                     /locus_tag="U4B03_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEN2068242.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3092..3463)
                     /locus_tag="U4B03_001384"
     CDS             complement(3092..3463)
                     /locus_tag="U4B03_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN2068243.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3482..4369)
                     /gene="xerD"
                     /locus_tag="U4B03_001385"
     CDS             complement(3482..4369)
                     /gene="xerD"
                     /locus_tag="U4B03_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEN2068244.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4465..4938)
                     /locus_tag="U4B03_001386"
     CDS             complement(4465..4938)
                     /locus_tag="U4B03_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2068245.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5043..5906)
                     /locus_tag="U4B03_001387"
     CDS             complement(5043..5906)
                     /locus_tag="U4B03_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2068246.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6102..8201)
                     /locus_tag="U4B03_001388"
     CDS             complement(6102..8201)
                     /locus_tag="U4B03_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN2068247.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8421..9530)
                     /gene="rpoD"
                     /locus_tag="U4B03_001389"
     CDS             complement(8421..9530)
                     /gene="rpoD"
                     /locus_tag="U4B03_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEN2068248.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9554..11395)
                     /locus_tag="U4B03_001390"
     CDS             complement(9554..11395)
                     /locus_tag="U4B03_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN2068249.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11577..13892)
                     /locus_tag="U4B03_001391"
     CDS             complement(11577..13892)
                     /locus_tag="U4B03_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2068250.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14077..14937)
                     /locus_tag="U4B03_001392"
     CDS             complement(14077..14937)
                     /locus_tag="U4B03_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEN2068251.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14996..15517)
                     /locus_tag="U4B03_001393"
     CDS             complement(14996..15517)
                     /locus_tag="U4B03_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEN2068252.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            15795..16556
                     /locus_tag="U4B03_001394"
     CDS             15795..16556
                     /locus_tag="U4B03_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2068253.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16582..19173
                     /gene="mprF"
                     /locus_tag="U4B03_001395"
     CDS             16582..19173
                     /gene="mprF"
                     /locus_tag="U4B03_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN2068254.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19361..20548)
                     /gene="tuf"
                     /locus_tag="U4B03_001396"
     CDS             complement(19361..20548)
                     /gene="tuf"
                     /locus_tag="U4B03_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN2068255.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20916..21272
                     /locus_tag="U4B03_001397"
     CDS             20916..21272
                     /locus_tag="U4B03_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2068256.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(21513..22517)
                     /locus_tag="U4B03_001398"
     CDS             complement(21513..22517)
                     /locus_tag="U4B03_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEN2068257.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            22683..23534
                     /locus_tag="U4B03_001399"
     CDS             22683..23534
                     /locus_tag="U4B03_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2068258.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            23551..23796
                     /locus_tag="U4B03_001400"
     CDS             23551..23796
                     /locus_tag="U4B03_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068259.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            23983..24687
                     /locus_tag="U4B03_001401"
     CDS             23983..24687
                     /locus_tag="U4B03_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068260.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         7028 a         5173 c         4355 g         8396 t
ORIGIN      
        1 taaatcaaaa aaatactcaa aacaaacaaa taaaacgttt acattattgc atgtcttgta
       61 tttttttcca aaaaaaaaga ccaagaaatt tcttgatctt tttgatattt atttgactgc
      121 tgtatgacgt tgttcgaact gatgttgttt tcgacttaac catggaagaa gttgcgcatg
      181 cagcactaaa aagacagcac tcacgatgaa tcctttgatc aagttgaaag gaacgatccc
      241 aatcagtaca tagtttgcta aagacatgcc cagcatctgt gttgcgttca gccctaaaaa
      301 cattaagtaa agaggcgtaa taacgaaata atttgcgata ctcataaaaa tagttagagc
      361 gatggttcct gtaatgacac ctaaaaattt gtttcttcct ttatgctgtt tctttacact
      421 aaagaaataa taaatcggga atgtaaagat cgttgttgat aagaaactag cagtgtctcc
      481 taccaaatta tccggagaaa aacctgtagt cagcagatgt aatactgaac gcaagaacgc
      541 tgttgcaact ccggccgctg gaccgaataa gaacatgctg atcaataccg agatatcact
      601 aaaatcgatc ttcaaaaaac taaaagccgg catgatcgga aaagcaaaat actgtaaaac
      661 taatgcgatc gcagcaaaca tcgctactgc aaccattttc tgtacacggg tgttcttcat
      721 ttgaaatcct cctgttagga ctcctcttca gcgaactttt tgacgtttct ctccaaatga
      781 aaaaactcta ttttccaggg aaaatagagt tagagttatt tgatcaagcg tcgtcaaata
      841 agctctatct tctttatcca gactatactg tcggtatcgg aatttcaccg attcagctat
      901 ttttttgcaa aataggtcgt ggactataac caccggtcgg gaatttcacc ctgcccctga
      961 agacgaacct tatataattt ttgtttacat attcattata catatctatt caaaaaacgc
     1021 aacatctaac acttataaaa agtaagtgtt cattttgagt ggttcagcaa actgctgact
     1081 tcttttttgt tcagttcacg gtaatcccca ggacgtaggc cttgtaaagt cagatctccg
     1141 tatttttcac gttttagttt ttgcacagga agaccaactg cttgaagcat atttttcact
     1201 tgatgattac gtccttcgtg gatcgtcaat tccacgatac tgtggtttgt cttaggatcc
     1261 actgataaaa tttggaattt agctggagat gtttttcgtc cttcgatctt tattcctttt
     1321 gtcaaaggtg caagcatcga ttttgtagcg atccctttta cctttgcaac gtaaacttta
     1381 tccacttcat gttttggatg tgtcaggcgc tgagcaaaat ctccatcatt tgtcagtagc
     1441 aagatccccg aagtatcgta atccagtcga cctacaggat agatacgttc ttttaccatc
     1501 ggcaaaagat ccgtaacaac ttttcttcct ttgtcatctg caacagcaga aataactcct
     1561 ttaggtttgt acaataaata gtaaccgtat tcttcttgat aaattgggac ttcatccact
     1621 tccacagtgt catgtttccc aacttttatc cctaattccc ttacaacttg tccgtttact
     1681 tttacacgtc ctgcaaggat gaattcttct gcttttcgac gagaagtgat gccagcgtgg
     1741 gcaattactt tctgcaatct ctccattaca attattctcc tttattcatt ccatcaaaga
     1801 atagatctgg tgacacgtct tcttcaagtg aaatttccat ttcttgtgta tcaggcagtt
     1861 cttctaatga tttcaaacca aaataatcta agaagtagcc agttgttcca tataatatcg
     1921 gccgacctgg tccttctact ctaccttttt cttcaatcag tttatgggca gctagacgct
     1981 gtaccgctcc tgaaacctga actcctcgaa tctgctcgat ttcagctctt gtgattggct
     2041 gtttataagc gatgattgct aaggtttcga cggcggcttg agataatgtg tttgatgtcc
     2101 cttgcgcata tcgcttaagt aagggagcca gctctttttt agtcgttaat acataatggc
     2161 tttccgtttc aaaaatatgc aaagctgagt gcgagttttg agtgtaagat tcttttaatt
     2221 ctttcactaa acttgtgact tctgcttcgc tgagatttaa cagtccgctt aattcttcat
     2281 tactgatccc ttcctcgcca acaataaaaa gaagggcttc aagttcactt aatttcgtca
     2341 tgttcggtat ctcctactgc tggataaagc ataattgatt catagttttc tttttgttgc
     2401 gcaatgatct gccctttttt catcaattct agcaaagcca taaaagtagt gacaacttca
     2461 cttttagaag gtgactcaaa aaaagaatcg aaagtacagc catgcgcatt tctttttata
     2521 cgctcttcga taaataccat tcgttcttcg atcgttgtgt catcgggcgt gatcgttttt
     2581 tccacttttt gagaaaattt cttacgttct aagattttct gcatggcacg gatcaaatcg
     2641 atttttttga cttgtcctcg cttcaacggc ttttcagcat ccgctaaccc atcgatgttc
     2701 ataggttctt ttgtgtaata aagacttcgt tcctctgctt tttctgtcag tctttcagct
     2761 gcatatttat atttacggta ttctaaaagc tgagtgacca attcttctcg aggatcttct
     2821 tcccataatt cttcatcgtc tgtgacgaat tcttggacag gaagcaatgt cttgcttttg
     2881 attgccatca atgtagcagc catcactaag tattctcctg caacagctag ctcgagtgtt
     2941 ttcattgtat ggatataatt catatattgc tccgtgatcg tagcgatcgg tatatcatat
     3001 atatcgattt ccatttgctg aatcagatga agcagcagat cgagaggacc ctcgaataca
     3061 tctaatttaa tattgatctc cgtcaactca ttcactcttt cttctttttc tttgctagcc
     3121 attttccaat gtaaggctct gtgatttcag ttgcacggat ggagacatcc gggtactttt
     3181 cctgtaattc atctaacatt tgaaagccca tattctcacc gcgataagac ggattcaaag
     3241 aaatgtcatg gatgatcagc tgatcgttcg ttgcccagta aataccaata atcccttgaa
     3301 tgttggttga accttctggc gtaaagaaaa aaagttcata atcgtcttca gattcgtacg
     3361 tatcaatctc cttcaacaaa gaagttttac ctgtcagttt cttatggaaa ctcaatagtc
     3421 ccatagcaat tttacggttt tctttccggt aatgtgttaa catcttctca ccttcctatg
     3481 actaagccct tggaaaatgc tgcttataaa catccgtcat tcttttcttc gtgatatgtg
     3541 tataaatctg agtcgtagag atatccgcgt gacctaacaa ttcctgtact gtccgcaaat
     3601 cagctccatt ttctagcaga tgtgtggcaa agctatgccg cagcgtatgt ggtgttactt
     3661 ctttgtaaat cccagcctct cgtactaact gcttcagatt tttccagatt ccttgccgag
     3721 aaagaccttt cccatgatta ttgacaaata catgcatttc agatgcgtcc tttacaagta
     3781 atggacgtgc ttcgtctaag tagcgttcca gccattggat cgcataatca cctaaaggaa
     3841 tgatccgctc tttatctcct tttccaactg tctgtaccag tccaagagaa agatgaagat
     3901 ctcccaattt caatccaatc aattcactga cgcgcatccc agtagcatac atgacttcca
     3961 agattgcacg atctcttatc cctaacgttt ttgtcgtatc aggtgcttca atcagctttt
     4021 ctacttctgt cagagtcaat gtacttggta atttctgtgc tttcttcggc gtatcgatat
     4081 gttgcatcgg atcatgatca gtgatacgct cttgccgtaa aaactgatgg aatcttctga
     4141 gacttgagat catccgagta acagttgctg tcgctttttt ttcttcatgc aacttctcca
     4201 gaaaacggat gactaaaaag cgatcgacat cctgccatga agcaattttc tgctcctgta
     4261 gaaaatgcaa gtactgttcc aaatcacgtt catagctttg tcgtgtattg gcggacaatc
     4321 cccgttcaat attcaagtag tggagataat cgttgatttg ctctttcata ataatccccc
     4381 tcatgcctct ctatcatatc agataccgct tcattctgat agtctttttc cataatctga
     4441 gcaaagaaaa agccgtctag acactcagac ggactggttt cttttttctt tttcatagca
     4501 ttcctgacag attccatgga acgttagccg atgatctttt actaaaaaat gataacgttc
     4561 ttctactact tgctccactt caagtaaaag atcctcttct acttcctcga tcgtgccgca
     4621 gttcaagcat agtaaatgat gatggaaatg tttggcgcct tcttttctca aatcataacg
     4681 ggctaatcca tcattaaaac ttactttatc gacaattttc aattctgtca agatttcaag
     4741 ggttcgataa acagtagcaa gtccaatttc cggactcttt tttttcacta aaaagaagat
     4801 ttcttctgca gataaatgct ctttctcatt ttccaaaagc acaagcagag tagcttctct
     4861 ttgcggtgtc agcttaaacc ccgcttcatg tagctgctgt tttgtttttt tcatcgccga
     4921 aatagcattc atgaccattt actcaacccc aacaatttat aataattaca aataaaaaaa
     4981 gatataacac tatgttaatt aaaattatta taagctagtt aagaaaaaca tgcaagggat
     5041 tctcaattag attttttgac tagttttgtc acaccgtttt cttgtgtaat caatccttgt
     5101 ttcaaaagat tacctaatgc gcgcttaaat gctcctttgc tgataccaaa agctttcatg
     5161 atttcttctg gatccgattt atctgtaaag ttgatttgct gatctgcaga acgttctaaa
     5221 atggttaaaa tcatttgtgc atcatctgat atagcttcat atgctcttgg cttcaatgaa
     5281 agattcagta caccgtctgg tcggacacca atcactcgac cttttatctg ctcgcctaga
     5341 cgaggttcat tatatcgttc agaaggatgg ataaacccaa gatagaaatc ttccgttaaa
     5401 acgtacgtac ctgttaattt caagcggtat gctgttccta tcacatcttt gtttttcatt
     5461 tcttctgttc catatttgct taaagatttg aatatttttt catctgctaa actgccccaa
     5521 atacggtctt ttgcatcaac tctaagggaa atcatcaagc gatccccttt tttaggccat
     5581 agctctgtca ttgtcggcaa ttcatcaaga gatacgacaa agtctttatc tggaagaccg
     5641 atatcgacaa acgcacctaa atcttttcgt gtagcagtaa cgattccaaa agcgtaatga
     5701 ccgatacggc ttttaggaat ttgtgtagtg atccgatttt catgtttttg attttggtaa
     5761 ccgaatcctt caactgcttc accgattttg tgttcacctt ctgatttgct taagccaaag
     5821 gtttgtccgt ttttttggac aaagtagttt ttttcatttt catcaataat aatacctgta
     5881 aatatagttg ctaataaatc gttcattttt ttctccctct atagtgaaag gttttcttaa
     5941 tacatttctt tgctatcata acataatgtt atctacttga atagctgagc cttggtttgg
     6001 aaaggtgaaa aaaggagtat gacaacagtt gtgtcatact cccctttttc gaataaacgg
     6061 tgttaaacgg caggctcaca gcctctttca tttgcctaaa attaattgct cccatttcct
     6121 ccgaaccatc cagttttaca gttgatggtt acgatgtaaa atggcaaagc actttctcca
     6181 atgcctttca tcaatgcaga ttttgttgaa gtggcatata gattataata gccttcccca
     6241 ttttcgattc tggctttttc taatctgtaa cctccttcaa cgatatatcc acgttggata
     6301 catgtagcaa tcactttgct ttggacacct ggtgcccacg tccatgctgg attagatgca
     6361 tcggcaatat cacttgctct ggcagatttg attggctgat tcgctgagtt tgaagaggaa
     6421 ggggtcgagg atgccgcttc ttgctgtgct gcttgtgcga ccgcagcttg ttgagcggct
     6481 tgagctgcag ctgctgcttg agcggcttgt tttgcttgtt cttcagctgc ttttttcgct
     6541 tcttctgctt ttttcttttc ctcttcttct tttttctctt tatcagtgag catcttatct
     6601 acttgtttca attgttcatt caactgagca gatagatctg tatttttcac tttttctact
     6661 gcttcttttg ccgtcttgta ttggtcgcgt gttgctgaat ccactacttt tccgtcttta
     6721 aagactatag ctgttttttc tttagctgtt tgcaattcag catattgatt tttagcttct
     6781 gtttgtgctt gttgaatcaa ttttccaaaa tctgtgttat ccgtttcgat tgcttggatc
     6841 tctttatctg ctttcaactt gatgtcatct gctagatgat catcgacgat ggcaggtgct
     6901 atgaaccact cattcacttt ttggatatct cggatcttag attggatatc ttggtaagtt
     6961 gtttctaact ctgaatatcc tttttcattc ttaagactgg ataaatcatt ttttagtttt
     7021 gataaatctt gatgaatcat atctgcacga ataaattgat ggtgatcatc tgtataaaag
     7081 gcagcaagtt tctcatgaag ttcttccatt tgttgctgtc tttttgcttc tgctgctact
     7141 tcggcttcta ctttttgatg atggtcataa taagcccagc ttcctccacc gagtagaagt
     7201 actgctgcta aaacgagata aggtactttt tttgattttt tcttttgcgc tttatctgga
     7261 tctgtttttt ctaagtttga ttcttgatgt gttttttcag tatctggcac gattgttatt
     7321 gatttcgctt ctgcttcaaa cagcttaggc tggttctctt ctttttctat ttttgcttca
     7381 agatcagtac cctctttgct tacagattga ttttcagcat cggattcact atttatttca
     7441 tcgggctctg ttgacttttt gtctgtgttt tcgagtattt cttttttctc tgcttccggc
     7501 agtttattcg ttttttcctt ttctaattgt ttttcagtaa atacttcagt tttttctgtg
     7561 atatccgttt ttacgtcttt tatttttact gtttctttac ctgtggcgtt ttctctatca
     7621 gagtcttttt cagtttcttg ttccttttct ggctctactt tgttttcctg tgcaattttt
     7681 tcactgatca ttttgagcag ctctttcgcg tgctcttctt ttgtatcttc acgatgtgct
     7741 ttgatataag cggatagtat cgaattttct tcctcattat cgacgatttc tttcagttca
     7801 gctactcttc ttttttgttt tgattcaagc gtttcttctt gattctttcc tgattcttcc
     7861 gaagtctcat gaacagttgc tttcttctgt tcatttttct ctatattagt tacttgatcg
     7921 tgagcaggtg gttcttcgtc ttctggttct gctttttcga ataattccat tacagattct
     7981 agaggtaaat ctttcaattc cgaccattcg attggatcgt ttaattctgt atcaggaaag
     8041 actgcatctt cagttgattt atcctgcata gctgcatctg tatttgtttc ttcaattgat
     8101 tcttttattt gctgatcttc tgcagttatt tgatgaccgc agtctggaca gatagtattc
     8161 tgctccattt ttttcgatcc gcaatttgga cattttttca tcacttagcg atcggctcct
     8221 ttactaggtt tacgctacca tttaatctat catattctat acgtcacgcc aatagcttat
     8281 ttttaggatt tacttaaaaa taatgaatga atttccttcc tatagaaaaa aaagcttgag
     8341 actgcgtagt ctcaagcttt ttttctttta aaataaacca aacagtttta ctgatttctc
     8401 ttctggtcaa tagtccacgc ttattctaag aaatctttta attgttttga acgagaagga
     8461 tggcgcaatt ttcttaatgc tttagcctcg atctgacgaa tacgttcccg agtaacacca
     8521 aatactttcc ctacttcttc caatgttctc gtccggccat catctaaacc aaaacgtaag
     8581 cgaaggacat tctcttcccg atcagtcaat gtatctagta catcttccaa ttgttctttt
     8641 agtaactcat aagctgcgtg ttctgctgga cttgttgctt cctggtcttc tatgaaatca
     8701 cctaagtgag agtcatcttc ttcaccgatt ggcgtttcaa gggaaacagg ttcttgcgcg
     8761 attttcaaga tttctcggac tttttctgtt ggaagatcca tttccgcacc gatttcttct
     8821 ggcgtaggtt ctcttccaag gtcttgcagc aattgacgtt ggatacgtat caatttatta
     8881 attgtttcaa ccatatgaac tggaatacgg atcgtacgtg cttggtcagc gattgctcga
     8941 gtgatggctt gacgaatcca ccatgtagca taggttgaaa acttaaatcc tttgcggtag
     9001 tcaaattttt caactgcctt catcagtccc atgtttcctt cttggatcaa gtcaaggaac
     9061 tgcatgcctc gtcctacata acgtttagcg atacttacta ctaaacgaag gtttgcttct
     9121 gccagacgct gtttcgcttc ttggtcgcct tcttcgattt tcaatgctaa cgctacttct
     9181 tcttccgcag ttaaaaggga gacacggcca atttctttca gatacatacg aaccgggtca
     9241 ttgattttta caccggttgg cgcagaaaga tcttctgttt ttgcttgttc agcttttttc
     9301 tcagcactct tcaagctgtg aattgatgga tcaccatttt catccaccac actgatacct
     9361 gcgtcttcaa tcttttggat caatttgtcc atttcatctg cattcaaagt gaatggtgta
     9421 gcaagctgat tggataaatc atcatataca acttgtcgtt tgggtttgtt ttctttgatg
     9481 aaagctgcta ctgctgcttc atacgttttt ttatctgttc catttgccat aaaagaaggc
     9541 cccccttgag tagctatttg gcttgtttta actgtttcgt taaattgatg atttcaatcg
     9601 tcaattcaag ctcaagctgt ttgtttccag ttcgtctggc ttcttgctgc atttgttttt
     9661 ttaatgttat ttcatcagct aagccagatt tgctgatcac atctagcaag tcttggattt
     9721 ctcgtggcga actttcttct gccaccggta aatattcgat ttccaccacc aaccgcctta
     9781 aattttcatc ttgcaaaaag tctaaaaact ctgcaaggtt gaattcataa tgttgtgaga
     9841 catatgcatc gaataaaaaa taaacttctg catatgcatc atgaataaac tgaaattctc
     9901 gttcttttaa aatattacgg acagaaactt ctttaaatgc acgatataat agaatctgct
     9961 ctgccttttc tacttgcgtc aaaggtctct ttttccgtgc agtttcttgt ctcgtaacct
    10021 gagtgtgttg actttcctga ggttcttgac gattttgctg atttgtcctt cgaagatcgc
    10081 gcagttgttt ttgtatcgtc tctctgccga tctgaaactc tgttgacagt tgagatagat
    10141 acatatcttg ttcgattggt gaaacaactc ggctaagttc aactagtaat tcttcgaggt
    10201 attcgatctt gtctttttca ttatctaaat ttttcccttg ttttaaataa cgtgatttaa
    10261 aagtaaagac tgtttcgcgc ccatgagcca ctaattcttg gaaagatgct tctccatact
    10321 tacggagata ttcatcagga tcaagctttt ctggaacgac tacgacagaa agtgctaatc
    10381 gactgttttg ccgaagcaat tcaatccctc gattggttgc ttcaaaacct gcgttgtctc
    10441 cgtcatagca aaagaccaat tctttcgtca gcttttccat ttgttggatc tgttgagctg
    10501 ttagacttgt ccccatagaa gctaatccag ttttgatacc agcctgccat gcagcaatca
    10561 catccataaa cccttcgaac aacaaaattt ctccgttttt ccggatatcg cttcttgctt
    10621 tatcaagatt aaataaaact tgtcttttat tgaacagttc tgtttctgga ctattcaagt
    10681 atttgggctg atctttttcc tgcccttttt caggtgcaag ccagcgtcct gaaaaaccga
    10741 tcgtctggcc ttgagaatta cgaagtggaa acatcactct ttgataaaaa cgatcagaca
    10801 tacggccatc atcatgcgtg acaaacaatc cagagcgttc taacaattca tttgaaattg
    10861 attcattctt gaatacttgc tgaagaaaag aacgctcatt aggtgcaaaa cctatgttga
    10921 attcttcaat taattctaaa gacaacccac gactttgcaa atattctaaa gctggctgtc
    10981 cagcttttgt atgcaatagc atgtgatgat agatttccgc tgctttttca tgagcagcaa
    11041 tcagtttccc cgtagcagat tcaggatttt ggacagctat agtacgctga cgccattgat
    11101 cttctaacgg tatctgttcg aagtctgcca cttttaccac tgcttctgga aaacttagac
    11161 cttctagttc ttgaagaaaa gtaaacacgt ttccgccttt accacaacca aagcaatgaa
    11221 aaatctgctt gtcttctgca acagaaaaag acggtgtacg ttcttcatga aaaggacaca
    11281 gccccaagta atttttacca gactttttca actggacata ttgtccaatc acctcaacga
    11341 tattcgtttg actgcgtact gtttcgataa cctcttgagg aatccgttga gccatttttt
    11401 caccccctgc tctattgata tcttttgggt ttaacagaag atatttcagg agaataaccg
    11461 gtaaaaaatc gcactaactg aaatcagtgc gattttaaag gacacaatta tacatgtttg
    11521 attgtatcat atttcattgc tttttcaagt ataacacatt tctatctcat taagcatcaa
    11581 tttgctactc ttttcagtaa ctgattttga atatcagcct tccagtacaa tactcgaaga
    11641 acaatattta cgagcatgaa tacaaagaag tatcctacgc tctctcccaa ccctaagttt
    11701 acttccaaaa ttccatggaa cagcattgca gctacatata tccctacgat cgtcgttact
    11761 gctaggaaga tgcgcaatgg gttgaaggga taacaagctt ttacaactgc catgcaactg
    11821 atcccaatca gcaagtaata catcaaagta gtagtttcag catttcccca tgcgaatgtt
    11881 tttccaagta gatacaccgc aatgatattc actactacaa gcaaggcatt tggtaaagca
    11941 ttaataagtg ctgtaggcaa ataacggtaa gtgatttttc ttttatcttg ttcaaaagat
    12001 aaaaagaagg aaggataccc ttcaatcgct aaatcaatca gcgtgatttg gatagggata
    12061 aacggaaatc caattgctgt aacggcacat ataatcgata aaataaatga ataaatcgtt
    12121 ttgataaaaa agatgcctga gactttcgtc acattgttga ccactcggcg tccttcaaac
    12181 aggacttctg gcaatgtagt aaaatcagag tccaacaata ccagattcgc aatctgacgt
    12241 gttgctccat caccttctgc catagcaatg ctgcaatctg cttctcttaa cgccaaaaca
    12301 tcattcacac cgtcgcctgt cattgcaacc gttctgccgc tttctttcaa ttcatttacc
    12361 agcaatttct tttgctgcgg actcactcgt ccaaaaaccg tatagcgatg agcagcttct
    12421 cgcacttcca tttcttcagt aatttgagaa agatcgacat acgcatcata attcggtaat
    12481 cctgcacgtc tagcgatatt ggagacagta aaaggattgt ctccagaaat cactttcaga
    12541 tccacacctt cctcatgaag ataagctagt gtttcttgag cgttttttcg gattggatca
    12601 tcgatttcaa acagagcaac cggctcaaca aatgctggtt tttcttcgtt caattgctga
    12661 tttggagcga tccccaacat aagtacgcga tagccattcg cttgggctgt ttttagactg
    12721 tccggaagcc gatcttcttc caccaaacgt tcaggcgctc ctacgtacac gactcccagt
    12781 tctggaaatt ccatcgcgcc ccatttgcga tcagaagaaa atgcgacttt gtttgtcaca
    12841 tcataccgat tagaaagctc aaaataatct ctcatagccc gcatagtgat gttgttatca
    12901 ctgctttctg ccagatacgt ccccataatt tctgggatgg ctttctcgta cagcggatgt
    12961 agtgcttcta cccgctggat actcattttc ccttcagtaa tggtccctgt tttatccaag
    13021 cataatgtat cgacatgggc cagcgtttca attgaataca tgtcttgaac aaggattcgt
    13081 ttttttgcca gtttggttac ccctgtcgtt aatgcaatac ttattagcaa taccaaaccc
    13141 tttggaagca tgcctaacaa agctgctgca gatgcaataa cagaaatttt gatagaagca
    13201 tcgcggatga acagtgcttc caaaaatagt atgatcccca aaggaataat cacgaaacta
    13261 gtgaatttgg atactttgcg aatcgaatct acaagttcag aacgaatagc tttgtgagtc
    13321 tttgcctcgc tagtcaagcg gacagcataa ttatctgcac ctactcggat aacttcagcc
    13381 atgacagaac cgcttgtcaa aaagcttcca gaaagcaatt cagctccagt ttctttgaca
    13441 atcaaatcag attcgcctgt caaaagagct tcatttgctt ctgcccgtcc agatataacc
    13501 tgtaaatcgg ctggtatctg ttctcctgct ccgattttta ccaaatctcc taataccagc
    13561 tctttagtag aaatcgattg ttctttaccg tcccggatca ctagtacttc tgattgggaa
    13621 acaatcgata atttccgtac aagatttctt gcatgtattt cttgataaat tccaataacg
    13681 atattgacga caataattgc taaatacgcc atgttggaat acgcacctac aaataacaga
    13741 caaatcccaa taccaaagtt cagaaaattg aataacgtca atatattatc gctgaatatt
    13801 tgttttgtac tttttgatgc atcttcctgg tagtcatttt gctggccaga agccattttc
    13861 tgaactacct cttgctgtgt aagtccattc attttctttt tctccatttt ccgccaactt
    13921 tcaaaaataa tcacgtgttt aagtctagcg tgttccaaga ttaaaaacaa cttgttttta
    13981 taaaatttat agaaaaacac acgaatgatt ttacaaaaaa gatgccaacc tttttgtttg
    14041 aatcacccgt gtgctgtttt tattatcaat ctatctttac ttatattgtt cctccaaacg
    14101 gctcatccac caacctagtg ctgtaccggc tataaaaaga acaggacaag ctagtacccc
    14161 agcaactccc aatcccgcgt aatcggtagg aatgatcata atcgttgaag caaaaactac
    14221 acctaaaatg aaatggaata gcttgctata tgcctttgag aaaatgtaat ccatgatttt
    14281 tgacaatcca agaacacaaa gaatcccacc aattccgatc ggcacaatca cttcaagtcg
    14341 tacttcttta aatccgtcag ccatcgcttt atacaatccc atatacacca aaaaattaga
    14401 agggctcaat cctgggacga tcatacccaa accaatcaat ccgccggcaa tcagccatgt
    14461 tccaaaattc tgatcgactt gcccgtcaaa taattgagcg ccgtataata aaaacaaata
    14521 agccagaaca aaagtaacaa ccatgatgat cacatcagaa gtatctcttc cttttttccc
    14581 tgcttctttc cataacgcag gaacagttcc agcgatacat ccgacaaaaa accataatat
    14641 cgttgtttca tacgcaccta ataaaaaact gactaaaaaa gaaagtagaa agatgcctgt
    14701 cactccgcct agaccaacag gcagaaaaaa caaaacattt tctttgaaat tttttgtaag
    14761 gtgggctaaa aaagaaatca gccgctcata catgccaaag acagctgcta atgcaccacc
    14821 gctgactcct ggcaagataa atcctgaacc gataaacatt cccttcacaa accgtaacat
    14881 ccagtctttg gttgtgtacc ccacttcttg attttccatt ttttgccttt tttccactat
    14941 ttgcgtactc cttctttcat ttaattttat tcatgattga acatttcctg ctattttact
    15001 taaagacttt ttcataggtc tctattattt tttcattcat tttgacaata tcaggataaa
    15061 acatgttacc tccattatga tagagatagc cgccattgta aagcaaggac tggatccgcc
    15121 aatatcggta ttgtttccct tcctcatttc ctaaaagtgg gctcagcaca tctttagaat
    15181 acgcttctgc taattctact gtgttttctc cgccatgatt ttttacatag gcaatataat
    15241 ctttcccaaa attatatgct tggacagctg tccataaatc gcatccttgt ttctttgctt
    15301 ctactatcat ttccgccaaa tatttgacac cttgatcaat actgtcagct ggattatcaa
    15361 tgatctcagc ttctccatgc agactttccg agctctgcat gagatctgtc ccttttcctt
    15421 ttgattcggt caatattatc gccttgatca aattctcgta gttttccata tgataatttt
    15481 ttgcagctgt tgttatttct atttgatagc tttccactcg ttttaccgaa cgataagttc
    15541 ttcctcataa aaacaacacc gcaccaataa ccgtcagaat gatcatgtaa aaaagaatac
    15601 ggataataga taatcgtttt cgcttcattt catcacctag ttattttgac atcttttttt
    15661 atcttactat ttctttatct tcttggctat ttcttccttg atttctgtgt acgccttaag
    15721 aaaaaatgaa ttttcctgta ttttttgaca tcttacgaaa atgatcgttg tttttcagta
    15781 aaaacaattg cccaatgact gatataatcg ttatacttta cacagtaggt gattatgtga
    15841 aaaaagaaaa gaaatatgca aaaatgccag atgggagcga aatttactat gaaaagagcg
    15901 gtcaaggctt ccctctcttt ttactgcatg gcaatgacgg cagcggccgc tttttttcag
    15961 aacaggtccc tgtactcgaa cgctactata ccgtgtatct agtagatagc cgaggacatg
    16021 gtcgttcgac aaacgaagca tctatgttga attttcagtt aatggccgag gatctgaata
    16081 cgatcatgtt gctggaaaaa atcgatcagg cagattttct tggatttagt gatggagcaa
    16141 atctagcact ggtatttgcc agcagcttcc ctaaaaaagt tcatcgattg attttaaatt
    16201 ctggaaacac gcttgttaaa ggcgttcgtt tttctgccag agtcatcagt aatattcatt
    16261 atgcatgggt atggttactc tctttatttc gcccaagtct tcgcaaaaat cttttagtta
    16321 tcaaactttt gctgcatgac atcgggctta cagaaaatga tttaaaaaaa atcaactccc
    16381 ccactttgat catcgtggga aaaaaagatg taatcaagct aaaacattct ttgtatatag
    16441 ctaaaactat accaaaagct tcctttgtgc ttgtgaaaga acaaggacat gaattagcta
    16501 gaaaagatcc tgaacgcttc aatcgagaag ttcttcaatt tctttctgaa acataactat
    16561 taggaataga aggtgattta tttgttaaaa aataccatac aatggctgaa aaaccaccta
    16621 ggactgttca aaacaatctt tttgatttct gtcattgtca ttattgtcgg tgaattgatg
    16681 tccatcggta aaactctatc tatccagcaa ttaggagaaa cgtttgccac tatacctgta
    16741 tggaaaagcg gactaatgct attgattggt cttatctctg tgttacctat ggttggttat
    16801 gatattattt tgaatcggat gcttgaacaa aagcagaatc ctcgttacct gtttgagaca
    16861 agctggctga tcaatacgat caacaatatt gccggtttcg gcgggtttgt tagtgtgggg
    16921 cttcgctctg aactatatgg acaaaagaaa gagagcagaa acgtgatcca agcattatcg
    16981 aaagttttct tgtttttgat ggctggactt tctatttaca gtctgctttc ttttttactt
    17041 gtagtcttca cgccagttgc tccttttttg aagcaatatt ggatatggtt gatcggcggc
    17101 ggtctatatt ttcctgtcgt ctttttattt actactttca aaaagaaagg attccttggt
    17161 ggtacctcac ttaaaactca aggacagcta ttgctagttt cattgttgga atggaccggt
    17221 gtcctgttta gtttcctttc tgttggttat ttaatggaaa tccggattga tctgtggcaa
    17281 acaattccat tattcattgc tgcttcagtg ataggaatcg tgtctatgat tccaggagaa
    17341 attgggagtt tcgatgtaat gatgattatc gggttgtccg ctattggtgt gccaagagaa
    17401 atagtcgttg tgtggatctt gctctatcgc ctgttctact acatcattcc gtttttgatt
    17461 ggaatcgtct ttttcttcaa aaatatcggt tccacttttg atcagcgtta ttctggtatc
    17521 cctaaacaat tagcaacaga aattgcgcat aaaatcgtgg ttgtcttatt atacttttct
    17581 ggaatcatgc tagtactttc tgccactatc ccgcaagctt ttacagagtt tcgctggctt
    17641 catagtttga acccattgaa atttcatttt attatccaat ttcctagtat actattggga
    17701 tttcttttaa tcattatggg cagagggatc gcagcacgtg tgaaacgagc atatttgcct
    17761 acaatcttct tgattgttct agctttactt tatgttttat taagtgattt tagtttcaca
    17821 ccggtggttt tcttgtctat tctgcttctg atcattcttg cttctaaaaa cgaattattt
    17881 cgggaacaat tgatctattc atgggagtgg cgtacgatcg atggaatcat tattggtgca
    17941 ctgtccttgc tttacatcgt tattggggta tacaatctgc cggatttccc tcatcggcgt
    18001 caccatttta tttccttttt cttatttcct tctgaaaaga tttggttttc gggattgttg
    18061 gccatcatcg ctgttagttt catgatcgtt ctatttgttc atttccttca aggagaaaag
    18121 aaacagattg gcgaagcctt taatgaagaa aaggccctga agatccttac aacttatgga
    18181 ggaaactccg atagtcaatt gatcttctta aaagataagc ggatgtttgc ctatgaaaaa
    18241 gacggtgaac caactgttct tctccaattc gcttgcttca ataataaatg tattgtaatg
    18301 ggtgatcctt ctggtaagaa agaagatttt ccagaagcaa tcgaagcttt tatcgaagaa
    18361 acagatcgac tatgctattt accagttttc tacgaaacaa gcgaagagat tgttatgatt
    18421 ttgcatgaat tcggctacga tttcattaag atgggggaag aagcttatgt agacctgaat
    18481 agttttacaa cttcagggaa aaaaatgaag ggcactcgtg ctgtcttgaa tcggatcgaa
    18541 cgagaaggat ttacatttga tgtcttacaa ccaccatttt ctactgagca aatgagtact
    18601 ttcaaaaata tctctgataa ttggttgggt tcacgaaagg aaaaagggtt ttctttaggc
    18661 tttttctctg aagattatct acaaagagcg cctattgcag ttgtcaaaaa tacccaagaa
    18721 gaaattgtcg cttttgcaac gatcatgcct acttacacga acaataaagt aggaacgatt
    18781 gatctgatgc gatatgattc taaaaaagca ccttctggca gtatggattt cttgttcttg
    18841 catttgtttg cctatatgaa agaagaacat atccaatggt tcaatttagg gatggcccct
    18901 ctttcaaatg tcggaacttc cagaaagagt tttttacagg aaaggattgc atacttggtt
    18961 tacgagtttg gttctcattt ttattctttc catggattaa gagaatataa aagcaagtac
    19021 gctacaaact gggtctcacg ttatactcta tattctagag atagttggat tgcatatgtt
    19081 atgattgctt tgctgattat tgataatgcg ccagttgagc agacacaagg tagaataagt
    19141 ggtttgaaaa aatggatacg aagaaagtat taaaaactgc ataccgatag atgaacactc
    19201 tcatgatgaa taaaagagac tgaagtaaaa cgaaagttca tgcttctaca atctcttctt
    19261 atacaattga agaacaaaga gcggtccaaa cagaaatcct gtttggaccg ctcttttatt
    19321 ttaaacattt ttcattacca aaatattcga gtcgcgtttc ttatgcttcg atctctgtaa
    19381 cgatacctga acctactgtc cgtccgcctt cacgaattga gaatgtcgtt ccgttttcaa
    19441 cagcgattgg atggatcaag tcaacatcaa tcgtcacgtt gtcaccaggc atgaccattt
    19501 ctgtatcttc tggcaatgtg attgttcctg taacatcagt tgtgcggaag tagaactgtg
    19561 gacgatagtt gttgaagaat ggagtatgac gtccaccttc ttcttttgtc aacacgtaca
    19621 cttctgcttt gaattttgta tgtggagtaa tggaacctgg tttagcaagt acttgtccac
    19681 gttcgatatc gtcacgttgg atcccccgta acaacacgcc tacgttatcc ccagcttccc
    19741 cgtaatctaa cgttttacgg aacatttcta cacctgttac aactgctttt tgtgtttcag
    19801 gtttgatccc tacgatctct acctcatcac cgacacgaac agcaccacga tcaatacgac
    19861 ctgaggcaac agttcctcga cctgtaattg agaagacgtc ttccactggc aatagtaatg
    19921 gtttatctgt atcacgttct ggtgttggga tatattcatc tactgtatcc atcaattcca
    19981 taatagcagc ttcagcatct ggatcgcctt gcaatgcttt taatgcggaa cctttgatca
    20041 caggagtgtc atcgcctggg aaaccatatt cgctcaataa ctcgcgaact tccatttcta
    20101 ccaaatcgat caattcttca tcatcgacta aatcaacttt gttcaagaaa acaatcaggt
    20161 attttacacc aacttggcgt gacaataaaa tgtgttcacg tgtttgaggc atcggaccgt
    20221 cagttgcaga aacaactaag attgcaccat ccatttgtgc tgcccctgta atcatgtttt
    20281 tcacatagtc cgcgtgtcct ggtgcatcaa tatgcgcgta gtggcgtttt tctgtttcat
    20341 attctacgtg tgcggtattg atcgtaatcc cacgttcacg ttcttctgga gctgcatcaa
    20401 tactagcata gtcttgagga tttgctaggc cttttttgcc taatactgta gtgatcgcag
    20461 cagttaaagt agttttccca tggtcaacgt gtccaatcgt accaatgtta acgtgtggtt
    20521 tacttctgtc ataatgttct tttgccataa tttacaaacc tcctaaataa tatagatcac
    20581 tgaaaaattt cagctctcac ctttttatta tagtcttttt ttgactaaat caaaaagaat
    20641 acgtccgact tactttttgt aagattcgaa ctaagcttat tatacgtcaa tattggtcaa
    20701 agtcaactaa gagcactcgt ctgtcttata cgcgaaaaaa gtacctttta taaataaaaa
    20761 aatgtgtttt ttgagaaaaa cttcaagata gtccttaact ttcttttgaa gcaataagaa
    20821 caaaatgagg aaaagtttaa aattcagctg ttgccaacaa ctttcaatag acgaaatctt
    20881 ctatttcgtt aataataagg aaagaggtga agtacatgaa aaaattaatt ggcgtacttg
    20941 tcgttttagg catatttctc ggcggaagtt ttgcagctta tcattatttt tacggcggcg
    21001 aagcttatta tacaaaaatc acgacgaatg gcgaaatgtc agctacccaa gcagataacg
    21061 gagagaaatt cacgacttat acctacaagc aagcggctta taataaagat ggtgaaaaaa
    21121 aacaggtgac gcttcgcgaa gaaagagaac atccattaaa gatgaatgcg tatcttaaac
    21181 tcaaagtcaa tcctcgtaaa ggtgtattaa gctgggaaga aataaaggct tctgaagtgc
    21241 ctaaaaatgc ggcagaaaaa atcgataatt agtaaatggt cttaaaattt tattagataa
    21301 aaagaggctg ggacagaagc gtttaactcc aagaaataag aaggagttgc ttctgcttcc
    21361 gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt ctcacgctct
    21421 ttttttgtta tattcgtctt ttccgatcct tctcttgaca caattcattt aactgatcac
    21481 tggtacttga acacctaata ttctagtcat ttttataaac gattcaatac ttcatccaca
    21541 tttttgatca tcacggatat cgtaccatcc ggattattca caaattcaat caaatctggg
    21601 ttgcgatata catctactgg tacgatcagt tcgatcccgt ttgatagctt gaatttttgt
    21661 tttccaaatt ttttctcaga aatttcttgt acttctttta caggaggtgc ttctgggaca
    21721 aatccttgct cttgtacttc ttctttaaaa gccatttttg ccgtgatgtt ttccttgaag
    21781 actttttccg caatcatctc atgatccaac cgaccttttt cttctatcgt atcatagaca
    21841 gcttctttca aatcagccat gatatcaaat tcttctgtct caaatttttt tccaagatgc
    21901 ttggctactt tttggatcac ttttacattt tcttctaaag aaggtgcagg aacactttcg
    21961 atcactcttc cagaaaaata aagctctttt ttgccagaaa actcatattt tttttcaatt
    22021 aattcgtagg tccaatcagt caaattcaca gcgaatgctt catctgcttt ttgtgttttc
    22081 gcccctagaa ttgctcgatt caaaatcaag cggttgtcga tccctgcttc gtctgcttca
    22141 acaaagtgag tgtaagcctc acgataattg actttcaata aagataagta catcactgta
    22201 tctaattcat acaagacaac aaatacatct gcactaggtg cttcttcact ttcttggtag
    22261 atatcgaacc accgctgaac gattgcttca cttgcttcca caaaattttc ttgagcgatt
    22321 ttaagtgatt caacgatcat gctttcttct gctagttgtc cagtttttgt ttgagcagaa
    22381 gagatttttt ggatcttctt ttgaagaaaa tcacgaatat attcttttgt cagatccagc
    22441 tctttttgtg aaaagactgg agaaccagtt tctctgtcaa taatatgcag aattgctttt
    22501 tttaaataga tatccatttt ctcattccct tctgtcttag tttacggaaa aaaaatcaaa
    22561 aataccagaa tcaaaatcat tttcttttaa acaaaaattt caagagtaga aacaaaatgg
    22621 attctttttt tcgaaaaatg cgctacactt atagttgaac gaataaaaaa ggagctgaaa
    22681 gtatgaattc attaacaagc acttatcgtc tatcaaatgg ttacgaaatc ccggtagtag
    22741 gattcggcac atggcaaaca cctgatgggg atgtcgctgt ttcctctgtt aaagaagcgc
    22801 tggctgcagg ttatcgccat atcgatactg cccaaggata caagaatgaa gaaagcgtcg
    22861 gccaagcgat taaggaaagt ggaattccta gagaagagat attcttgaca acaaaattat
    22921 ggaatgcaaa ccacagctat gaacttgtga tgtcttcttt tgaagaatca ttgaagaagc
    22981 tccaaacaga ttatttagat ctgtttttga tccactggcc aaaccctgtt gcttttcgtg
    23041 ataattggga acaagcaaat gcagatacat ggcgagcatt tgaagaactg tatgaagcag
    23101 gtaaaatcaa agcaatcggt gtgagcaatt tcttacccca ccatctagat acactagcaa
    23161 aaacagcaaa aattatgccg atggtcaatc aagttttcct tgcaccaggc gaattacaga
    23221 caactgtagt tgagtatgca aaaaaacatg atatgatcct cgaagcatat agtccattag
    23281 gcactggtaa aatcttcgat gtgcctgaaa tgaaacaaat cgctgaagca catgacaaga
    23341 cgattgcaca agttgctttg cgttggtcgc tgcaacacga attccttcct cttcctaagt
    23401 cagtgacacc aagcagaatc aaagaaaata ctgaattatt tgattttgaa ttaacagaag
    23461 aagaaatgaa acaaatcgat cagttagatg gtgtagtcgg caaagcaaaa gacccagata
    23521 ctacccaatt ttaagtcgaa aggtgatttt atggatccaa tcgaacgctt gaattctctc
    23581 tcggaagaag ttacccagac ttttcattcc gattttgtct ttttgatcga tgcagagaaa
    23641 atccaacatt ttcctgcaag aaactggaca catgatcaaa tcatcgaaga actgaaaaaa
    23701 cggttcgatc attcgctaat ggtcaaaccc tggcacgaac atgaagtcat ttattcacct
    23761 gaactacctg tttttgcctt gataccgaaa aaatagaaag aatggaacgg attatgtata
    23821 tatacataat ccgttccatt ttttattttt ctttcaaaaa acgggaaaat acgcaaattt
    23881 ttctgttgta atttgcgtat acgcaagttt ttttgcatta ctccttttca tttggctgtt
    23941 tttttcttat aatcgaaagg gtatgagcga aggagagaac atatgaagaa aaaacagaaa
    24001 caaaaattat taaatcaatt ccgtccttca ttggatggtg taagaggaca attattccgt
    24061 tctctagaag aagagagctt gcgacgttat ggtttgccgt tacaagtcat gcttgatccc
    24121 aaaaaccgtc aaatcttaac gatcggatta gctgggaaaa caaatgaaga tacacggatc
    24181 aacgtgccaa tcgatgacaa cttcaatact gtattaaaga gaattcgttc aggagaaaac
    24241 gggattttcg aacgattccg tgacaatctt cttattgaaa tcgtgtctta ctggaatgat
    24301 caacgattga aaaacgatga accaactgcg caagctgttg cgacaccagt tacagaagaa
    24361 acagtcagtg tgccagaaga aaaaacggaa aaaccaaaag cggacacaac tgaaaataca
    24421 cgctcaactg atacgaatgc cgctttaagt tttgctgatt tttcaaaagc cgttgctgag
    24481 tatcctaaat tttacacaga acaaaatgaa catgaagtag ttattaatga aaaaaatgct
    24541 gatgaacctc gtaaattagc aactatttct atgactactg aaaatgagtt cgttatcgaa
    24601 aaagcactcg aacggaaata caaagtcaaa ttgactttga ttcctttgat cgaacaattt
    24661 gcagcgacag cgattactga tcgataaaaa ttctatttgt cataaaaggc tgggatttaa
    24721 aatcccagcc tttttagtca tataataatt tctacagcaa gtaaaaagaa attccttcta
    24781 acatcaggct caaatatcag cttgacctgt ccaacaaatc acctatgatc ctacaatgtt
    24841 aggcgacttc tcttctacct gaagatatct tggagttgcc tgatttctta agatgcctaa
    24901 ccttaactat aaaaaattct aataataaac atattttctg atagagaaaa aa
//
LOCUS       DASJSZ010000037        24895 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.037, whole genome shotgun sequence.
ACCESSION   DASJSZ010000037 DASJSZ010000000
VERSION     DASJSZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.037"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..218)
                     /locus_tag="U4B03_001402"
     CDS             complement(<1..218)
                     /locus_tag="U4B03_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN2068261.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIE"
     gene            complement(348..932)
                     /locus_tag="U4B03_001403"
     CDS             complement(348..932)
                     /locus_tag="U4B03_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2068262.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1263..1496)
                     /locus_tag="U4B03_001404"
     CDS             complement(1263..1496)
                     /locus_tag="U4B03_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEN2068263.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1511..2461)
                     /locus_tag="U4B03_001405"
     CDS             complement(1511..2461)
                     /locus_tag="U4B03_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN2068264.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2458..3300)
                     /locus_tag="U4B03_001406"
     CDS             complement(2458..3300)
                     /locus_tag="U4B03_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2068265.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(3302..4021)
                     /locus_tag="U4B03_001407"
     CDS             complement(3302..4021)
                     /locus_tag="U4B03_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2068266.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            4713..4871
                     /locus_tag="U4B03_001408"
     CDS             4713..4871
                     /locus_tag="U4B03_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2068267.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            4943..6169
                     /locus_tag="U4B03_001409"
     CDS             4943..6169
                     /locus_tag="U4B03_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2068268.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(6257..6649)
                     /gene="rpsI"
                     /locus_tag="U4B03_001410"
     CDS             complement(6257..6649)
                     /gene="rpsI"
                     /locus_tag="U4B03_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN2068269.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(6665..7108)
                     /gene="rplM"
                     /locus_tag="U4B03_001411"
     CDS             complement(6665..7108)
                     /gene="rplM"
                     /locus_tag="U4B03_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEN2068270.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(7627..8094)
                     /locus_tag="U4B03_001412"
     CDS             complement(7627..8094)
                     /locus_tag="U4B03_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN2068271.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(8214..9752)
                     /locus_tag="U4B03_001413"
     CDS             complement(8214..9752)
                     /locus_tag="U4B03_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEN2068272.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9832..10518
                     /locus_tag="U4B03_001414"
     CDS             9832..10518
                     /locus_tag="U4B03_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEN2068273.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(10719..11246)
                     /gene="msrA"
                     /locus_tag="U4B03_001415"
     CDS             complement(10719..11246)
                     /gene="msrA"
                     /locus_tag="U4B03_001415"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2068274.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(11330..12007)
                     /gene="pgmB"
                     /locus_tag="U4B03_001416"
     CDS             complement(11330..12007)
                     /gene="pgmB"
                     /locus_tag="U4B03_001416"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN2068275.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(12004..13134)
                     /locus_tag="U4B03_001417"
     CDS             complement(12004..13134)
                     /locus_tag="U4B03_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2068276.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(13134..15317)
                     /locus_tag="U4B03_001418"
     CDS             complement(13134..15317)
                     /locus_tag="U4B03_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN2068277.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(15314..16339)
                     /locus_tag="U4B03_001419"
     CDS             complement(15314..16339)
                     /locus_tag="U4B03_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2068278.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(16384..17211)
                     /locus_tag="U4B03_001420"
     CDS             complement(16384..17211)
                     /locus_tag="U4B03_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2068279.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(17208..18275)
                     /locus_tag="U4B03_001421"
     CDS             complement(17208..18275)
                     /locus_tag="U4B03_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEN2068280.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(18301..19137)
                     /locus_tag="U4B03_001422"
     CDS             complement(18301..19137)
                     /locus_tag="U4B03_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2068281.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(19134..20003)
                     /locus_tag="U4B03_001423"
     CDS             complement(19134..20003)
                     /locus_tag="U4B03_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2068282.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(20073..21356)
                     /locus_tag="U4B03_001424"
     CDS             complement(20073..21356)
                     /locus_tag="U4B03_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN2068283.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(21370..23055)
                     /locus_tag="U4B03_001425"
     CDS             complement(21370..23055)
                     /locus_tag="U4B03_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEN2068284.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            23330..24355
                     /locus_tag="U4B03_001426"
     CDS             23330..24355
                     /locus_tag="U4B03_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2068285.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         7404 a         5076 c         4226 g         8189 t
ORIGIN      
        1 aactctatct tgatagcatc aataattttc attactaaaa acagaactga ataaatcaag
       61 atgaaataaa tacatgtctt tattttacga aacatcaata ctaaaactgt agcaataatg
      121 aaacacccca cttgaaatat atttcccttt cctaaaaaaa taatgaccgg aaaaaaaata
      181 tttaaagtaa tcaaggttaa cggattgatt tttgacactc tttcacctcc aaaaaataag
      241 atcgcactgt tatcttaagc tgataaaaat gcaccgttat tcagataaca gtgcgatctt
      301 atcaaaaagc ataactgaga acaattctca atgcaagctt tttttaatta actcagtaat
      361 tcaatccacc caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct
      421 tttgaatagc tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag
      481 tggatttctt cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta
      541 tctagacaac agactgtctt tttcgtcaca aatgatgtaa ttttttcaac aatatcttga
      601 tatttggttc cagctgattg gaataaaatc aaatcgaata tacgtttatt ctcaaaaata
      661 taattaataa atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata
      721 ttttctaagt ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta
      781 atatgcggct gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa
      841 gcacccgtag ttacgcctgc ggtcaggcac agttgcctta tcttagtttg ctgaaatcct
      901 tctttttcaa aatggcttag tccactttct attaactttt ggtgagttat aatataatct
      961 ctggtttcta tgggtacttc ctcctaataa gatgaattaa ctttattatc ttacacagga
     1021 cttgaagttt caatcgttaa cgtttattac tgaactcatt ccttagagct aaactaatga
     1081 gaaagagcag aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa
     1141 tcattcacct tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag
     1201 gataagtcct atcataatca ctccaatagc cattttctta tcctccctaa aaataaagtc
     1261 ttttacttaa aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga
     1321 aatcctatat ctaaatagtc ctcatatcta agtttagcca gcatctcact tacagatgct
     1381 ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat
     1441 aattcctgaa ttgctagtaa ataaattagt atagtagtcg ttttaatttg taccatacct
     1501 accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg
     1561 catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat
     1621 agataggaat attggtatct tttgatactg ttttcattgg tctatcatcc acactgcttt
     1681 ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat
     1741 ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg
     1801 ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa
     1861 ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca atatacttat
     1921 ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa
     1981 tatttttagc gtaaataata ccgttttcta aatttaacca agcatgagga tcttccttcc
     2041 ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt
     2101 ctttgttttc ctctttgttc gcatttttaa ctaattttgt aaaccaagca tttcctccag
     2161 tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt
     2221 caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa
     2281 ttttatcttt tgctatattt tcggtaatat ctgctaaaat cgaattagta actactactt
     2341 ttaatttttc gttcgaagcc actttatctt tgtctttact atttattgaa catgccccga
     2401 atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca
     2461 taacgatctc tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac
     2521 aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc
     2581 aataaacaat ccaagcaatg atgaacttgc tccgactgtt gcagaaataa atatcatagt
     2641 tttcaagcta tttgcatata aatatgctgt agctgctggt gtaattaaca ttgcaacaat
     2701 taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata gaaccatcaa
     2761 tagataatga taaaaactga cattcatccc tattgctttt gccattaatg gatcaaaaga
     2821 agtgattaat aattctttaa aaaatagaac tactataacc aacactaaaa ccgaaacgcc
     2881 aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata aaatatgaaa
     2941 taaatccgtt gagctatttg caactcctat aaggataact cctagtgcta aaaaagaact
     3001 gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat
     3061 aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat
     3121 ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat
     3181 tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt
     3241 aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat
     3301 attattcccc ctctccaata aatatagtat ctccataggc tttttttaaa ttttcttcat
     3361 taaatacaga ttctacactt ccgtgagcaa ttagatttcg attcaaaaga ataatattat
     3421 caaaatactt ctttaccttg cttaaatcat gatgcacaat tagcactgtt tttccctgat
     3481 gtttcaattt ttttaacgta tccataatta ttcgttcact aactaagtct atccctacaa
     3541 atggttcatc taaaaatatg aaatccgcat tttgagcaag acatcttgct aaaagtacac
     3601 gttgaaattg tcctccagat aattctccta tttgatgatt agaatatttt cccatatcga
     3661 ccttttccaa agcctttgaa actctttgcc attctgcatt tttaattctt tgaaaaactt
     3721 tcattccggc atatgttccc atagatacac attcttttac ttttattgga aaagtaaaat
     3781 caatatcagc tttttgctct acatatgcaa tcttcaccag ttttttattg atatcttcat
     3841 tattaatgga gacctctccc tgatgaggga taatatttaa cattgcttta agcaatgtgg
     3901 attttccagc accatttgga ccaataatac ctgttaaagc accttcttgt atttttaaag
     3961 aaatctcgtt caatgctaaa aaatcgttat aacaaacatt aagctttttc acttctaaca
     4021 taactttctc cttctaaatg ataatgatta tcacgatcat atggtataca aaaattatat
     4081 tttagtcaac cctaaatttt atagtttaga tttttttctg ttgctgaaga ttagaatgac
     4141 aaaactattt gaaactacgc gtatagtaag tagctttcaa aatatataaa aaaggagcgt
     4201 tctcaaatga gaacatgctc ctaaaactac tttattggcg tatggaaaaa ttatacttga
     4261 agtggatagc gggttgaata agttgataag catcaaaaca tttataaaaa ttattcaaga
     4321 ctatgtctgt tccatataca aatatgaaaa tcagtaaact ccatttaact tttttcgtag
     4381 tgtgtctttt tatacatgtt tatcgttatt aatagtgaat aggaaaaaaa ctatttttga
     4441 tcattgacaa ttaaatacaa tttttgaata acgatccctg ctattcgagc tagaaactca
     4501 agtacgccat gacctgatta gcgacaaata cttagagatt tctttttgtt ctgggaaaac
     4561 aaaatgccat gacaatagca gcaaataatg atacttccgt aaggctcgcc gtaaactggg
     4621 gagaggtgaa attcctatgt gcttacccaa agcaattcaa aaatttcata actactattt
     4681 taggagatga ttttatagaa tccagagtct acctgaataa aaaacaaacc tgtgcatttc
     4741 taggaatcga taataatact ctagataagt ggattcgtat atataatttc ccagtaataa
     4801 gagttggtgg tgtagttcgc tttgacgtag aagatattaa agactttatg aatacattta
     4861 aaataacttg aaattttctt ctcgccgcat atactaaaat tagtgaaata tactgcctta
     4921 ctttttttaa ggagtgagga ttttgcctac aaagctttca aatgggaaat ataaaactaa
     4981 tctgagatat ccaaaaaggt ttaaagaaat aacaggaatt gcatcagaaa agtatcaaaa
     5041 aacatttcca aataggcaat tggcgattaa agccgaaaat gatatgaaga agaaaataga
     5101 aaaagttttg agagaggaga atgcaaactc attagaatta aaaggaaaaa taacgtttaa
     5161 aaaattttac gaatctaaat ggcttcctag atatgaatta ggtcaaacaa ttagatcaaa
     5221 caggcctcct agtgatataa ccattagtaa tacaaaagat atatttcggt tgcatatttt
     5281 acctatgttt ggtgaatacg caatgaatta tctaaatatc aatactgaga taatatcaga
     5341 tgaattaacc aaaaaatcga aagaatatgc aaatataaaa atcataaaag gatatgttcg
     5401 ttccatgttc gatattgcag aaatactaaa ttatatcgaa tttaatcgaa ctactaaaat
     5461 tattcaaagt atcactgcac ctaaaaaaaa tgctttagaa gaaaaaagaa tacaagaagg
     5521 taagcaagca ctatcatcaa aagaacttac taattggata gaagcagtaa atgatgattt
     5581 taataatcat ttacttactt ttcatgatta cacacttttt atgattaccc tgtatcttgg
     5641 tgatagaaaa agtgagactt acgctttgca atggaaatat attgattttg aaaaacaaac
     5701 ggtccgtctt aaacatactt tagataaata tcaaagaaaa aagtttacaa aaggtcgaaa
     5761 agataccgtc attcaagtac cagaagttgt aatgacattg ctaagtgaat ggaaatcggt
     5821 acaagctgat caattgttga aattaaaaat taagcaaacg ttagatcagt atctctttac
     5881 ttatactaaa ccatctggag aagtaaattg tcctgttcat gccgattacc taaactatcg
     5941 aatcaattca attaaaagaa ggcatcctga cttagttcat ttatctccac acaaactccg
     6001 tcatacttat gcaactatag ctagacaagg tggagcagat atgaatcaaa tttcaaatgc
     6061 tttaacacac tcagatattt ctacaacaaa gatatatgtg aacacaccag atatagttga
     6121 taaggctgtt tttgaagctt ttcagagagg attgaataag tgtgattgat atgtgttctt
     6181 aaatgtgttc ttaaaaacaa aagaagcact ttccaaataa ttgaaaagtg ccagaaacgt
     6241 tgatatataa aggatattaa cgttttgaga attgtgaagc tttacgtgct ttcttaagac
     6301 ctggtttttt acgttcgacc atacgtgcgt cacgagtaag taagccagca cgtttaagag
     6361 ctaaacggaa gtcaggatca acttctagca atgcacgagc gataccatga cggatagctc
     6421 ctgattgtcc agcgtatcct ccgccgttta cgttaacaaa tacgtcatat gcgccttttg
     6481 tttcagtaac agcgaaaggt tgattgatta cttcacgcaa gtcagcatgt ggaatgtatt
     6541 cttcaacatc ttttttattt acagtaattt tgccagttcc tggtactaga cgtacgcgag
     6601 caactgcgtt tttacgacgg cctgtgccga tatattgaac ttgtgccaat gaaattccct
     6661 cctattagat taagtttgtg atgtctaaaa cttctggttg ttgtgctgca tgtggatgtt
     6721 ctgctccacc gtatacgttc aattttgtaa attgtttgcg gcctaaagtg tttttaggaa
     6781 gcataccttt tacagaagtt tcgatcaaac gacgagaatt tttagcacgc aattcgccag
     6841 cagtgattga tttcaatcct cctgggtagt tgctgtgacg gtaataaatt ttgtcagtcg
     6901 cttttttacc tgttaatttc actttgtctg cattgatgac gatgacgaaa tcgccagtat
     6961 ccaaatgagg tgtgaaagtt ggtttatttt taccacgtag tacagatgca acaactgttg
     7021 aaagacgtcc caatggaaca tcagttgcgt ctactacata ccatttacgt tctacttcgc
     7081 ctggtttggc catatatgtt gtacgcacgt tattttcctc caatttaagt tctgatgttt
     7141 gacatacact tcgagtttcc ggggctcttt gtggggcaaa caataccatt taacatatta
     7201 cccgctaatc tagtcaatgt caacgttttt ctgcttgatt gaaaaattta tatgaaactt
     7261 tacttctttt ctttctttta tataaaaggg tcgtttcctg tcaaaatgaa aaacaaaaag
     7321 aggctgtgac aaaagtcttg tcacaacccc ttattttgaa taaacggtgt tctcctgcca
     7381 actcctcggt cacaagtcac aatatccata aaacataaga agctgttttt taaaattgtt
     7441 tcttgtgact cggagctgaa cggcaggctt acaacctctt ggtaaacggt gttcaatcag
     7501 ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt gacgtttgtt
     7561 tctctaatac tcaggtcaaa acgctgattt cacaacctct ttttcggtca agttcaacaa
     7621 cagtttttac gcgtcgatct ctggcgcttt gcccaaatga gcttggatca tccagatacg
     7681 tttttctgtg gctgttttga attcgataaa gatatcttgt gttggatcgt cgccttcttc
     7741 tccagaaact tcgattcctt tttggtaaag atctgctaga taacgatagc ctgcaacaag
     7801 tgtttccaaa cgttctggaa tagtacgatc ccagcggcca atttcatcag ggatttttgt
     7861 gttatctgca aattcacgca atgtagaata aggtgaaccg tctaatgtga tcaagcgttc
     7921 ggatacttca tctaactgag cgttcaaatc atccatgaac tcatccatca ttgggtgcaa
     7981 tgtaaggaaa cctggtcctc tcatgtacca atgtgtttgg tgaacaacaa cagacatttg
     8041 gcttagatct gcaactaatt gatttaatac ttctttggtt tgttggaatt tcatctttct
     8101 tgcctcctaa cattttctta cacttaaaat aatagtagac tttcaatgaa aagtacaaca
     8161 aaatcattta cctgagaacg attttcagtt cgctttttta tccgtaaagc caatcatttt
     8221 tcagaatcag ctaattcttc tcgcagttga gcaaattgct gataagtctg tgctaaatgc
     8281 ttttgtgtcg aaacaaataa tggatagatt ttctgatatt ttttatgatc agtttcttgt
     8341 actccaactt gctctttcaa ttctatcaat tcctgtgctt cactgatact ttggatcttt
     8401 ccgatgcttc tccagcctaa aatcacagct cctaatgcgg atgcttctgt gacagtaggg
     8461 aaaacaagcg ggtaacccag tacatcagcc atcatctgac gaaaaatcgg agattgagca
     8521 aacccgcctg tagcacggat ctctgtgagc tgattttcct ggcaaactcc ttttaaaatc
     8581 cgataaaggt tgagacagat cccttctaga atcgccttca tcatttcttt ttctgtatgt
     8641 tgccgctgta aaccgataaa actgccaaac gcatctgcgt cccaaagcgg tgctcgctca
     8701 ccaagtaaat agggatgaaa aatcagacca ttggcacccg ctggcacaaa agcgatagcc
     8761 gaaagcagtg catcatatgg attttctcct ttttctttag cagattgaac gacttcctgt
     8821 aaaaatgtat gccctgccca atcaaagatg ccagcaccat tgctagtcgc tcctcccact
     8881 acccaatgtt tctgatccag aacataacaa aacgtctcca tttctggatg gaactgaggc
     8941 tgagtagtaa taaaacgaag tgctccgctc gttcctacgg tcaaagcggc gatcgtatca
     9001 ccaaaagcac ctaaccctag attagaaagc ggcccgtctg cccctcccaa aacaaatgat
     9061 atatcatttg gcaaacctag ttctgctgct gtttttgatg gtagacctgt tacttgatag
     9121 gtggaaggaa agacttgcgg caattgctct attcgaatgt tcaagtagtc aagcacttct
     9181 tctgcccatt ggaattcatg gatatcaaaa tatccagttc ctgaagctgt tccgtagtcg
     9241 ctgatcagtt cgccaaacaa tctataaaag atatagtctt tgataccgac aaaataagca
     9301 gtttttttga ataattcagg ttggctgttt tgcagccact tgattttgtt caatggtgtc
     9361 attggatgaa tcggcgtccc agtctgccga taaaattctt tgccaagcac ggtatatttc
     9421 agtttttctg cttcgtccgc tgctcggtta tctgcccaag tgatcattcg agataacggt
     9481 gttttctctt catccaacaa tatcaggcca tgcatcgcag aggagaagga gacaaattga
     9541 atgattgtgt ctgaaggcaa ctgtttaatt acttctttta taacggtgca gacaccttga
     9601 aagatcaatt cctgatcttg ttctgccatc ccttgtgttt cttggatcaa agggtagcga
     9661 aaataagcgg atgctgcttc ttgggcttca tctgtgaaag caactgcttt ggtttgtgtg
     9721 gttccgatat ctaatccgat tgaaacgttc attttttcac ctcaaatttt atgtatgtac
     9781 aaaaatttag tttaagcttg cgtatcttgg ttttgaaaag ggggagatgg cgtgttactt
     9841 tattttatca ttggttgctg tatcggatca tttttatgtt taaccgccca gcggattccg
     9901 ttaggtcact ctattattta tcctagatct cattgtgtca aatgctgtcg ttcgctttct
     9961 tggtatgaat tgattccgat tctctcgatc atcgtccaac gatttcgctg ccgctattgc
    10021 aggtgtcggt tgcccttcta ctatttgttg gcagaagcct tatgtggtgg tctttttgcc
    10081 tggttcttta ctttttcttc cgcaagaaat ttttcgacgt tcatatggat gctttcggct
    10141 ttgctatttt cgttgatgga tttattttac ttcatggtcc attctcacac gctattctgt
    10201 tcttgggcaa ttttatggat tttttggttg caggccggcg tcttccaatg gcaaagtgtg
    10261 ctgttaacgt ttatcgtagg cggtgtgtgc cttcgctatg gtcaatccta tctcggagct
    10321 ggcgatactc tgttgatgct ttcttggagt ggtggcttgt ctctagaaga gttgcttcag
    10381 atcttgtttc ttgctagtct gtttggaata tccgtttttt ctgtttactt tatctgtcat
    10441 aaaaaaaagt tggaaaagct gcctttcatt ccttttttga gcagtgcgtt gctgattgtc
    10501 cttcatctta ggtattgaaa aaaggttgtg acaaacgact tgtcacaacc tctgatatat
    10561 agtgttctct taccaaatcc ttgtgacttg gagctaggcg acaggttcac aacctctcca
    10621 ctaaatggtg ttcaatcagc tgaccctcag tattagctca aaatgctggt tttacaacct
    10681 ctgatatacg gtgttccata agtagctttt tcggcttctt atttcttgac gccccaatgt
    10741 cgacgaagga aacctgcacg tccagaacct tctcgataaa gattgtaatg gagcttgttt
    10801 ttcttgtaat aatcctgatg gtaatcttct gcaggataaa attcgcttct tggttcgatt
    10861 tttgtcacga ttggcttatc gaatcgcccg ctgtcttgca atgcttgttt tgaacgctct
    10921 gcaatatttc gttcttcttc atcattatag tagatcactg gacgataaga atcaccgcga
    10981 tcagcgaact gtcctaatgc gtcagtcgga tcggtttgct gccaatagat ttccaccaac
    11041 tgatcataag aaataacttc tggatcatat tcgatctcta ctgcttctgt atgccctgtt
    11101 gtgcctgttg ttacttgttc ataagttgga tttggtacat gcccgccagt ataaccggat
    11161 gtcacagaaa tgatccctgg ctgctcttca aatggtttga ccatacacca aaagcatccg
    11221 cctgcgaaaa ctgctttttt tgtcatataa aatccccctt ttttctcatc ataccttgat
    11281 ttccaaaagg aggataacgt tttgcttgtg acagaaaaaa cggaaactat tatatacgtg
    11341 atacggcttc tgataaatgc caaaacgaac gtacttgatc aaaaacggtt aaatcaggaa
    11401 tcacatattc agcagaagga agatttatgt tttcacctat tcccaataca tgcatgttac
    11461 cagccaaagc tgcttgacaa ccagcttctg aatcttcaat gaccaagcat gcatttggcg
    11521 gtacattcaa ttgctgtgct cctttgagaa aaacttctgg atcaggtttt gcttttgata
    11581 cttgcgtgcc gtcaataagg acatcaaaat aacttgtcaa attcgttttt tctaaaatga
    11641 ggcgtgcatt tttgcttgct gatcccaatc cgatcttgat gtttgttttt ttcaagtact
    11701 caagaacagc tattgcacca ggcaaaacgg atgtttcatc cattttttga atcgcttgaa
    11761 gatatagatt gtttttttgc tcagcaaagg ctgatttctc tttttctgag aatttctgtt
    11821 ccatttttcc aaatgacagc aatcgctcta gtgactcggt tctggatatt cctttcagtt
    11881 gctcgttttc tttctcagtg aatggaatgg acagctggtt agctaaaacc agccacgctt
    11941 cgtaatgata acgagctgtg tctacaagaa cgccatctaa atcgaatagt gctgctttga
    12001 ttttcatcga ttacctcctt gaaaatgaac gacttgaagt aaagatgcga tgtttttagc
    12061 tgtgcgatga agattatcag tggcttgctg taaagcttct tgtaaaggca cagctgcagg
    12121 aaggatcgga aaaacggccg aaatgccaac atctgctgct ttttcgcttc ctttaccaac
    12181 acttccgcaa atagctatca caggaacgcc ctctggtact cttctagcaa tcccaactgg
    12241 cgcttttcct tttagacttt gtccatctag acgtccttcg cctacgatta cgaggtctgc
    12301 ttgctgtacc cgtttatcga actccagtaa atccaatacg tagtcgatgc cagagactat
    12361 tttccctgaa gcaaatgcta acagtcctgc acccattcca ccaccagcac tactgcccga
    12421 tgcctcgatt acttgagggg caaatctcat ataaaaatca tgcatggctt ggtcagccac
    12481 tgctaattca ttttctttca agcctttttg tggtccgaaa acaaaagaag cgccttcttt
    12541 tccgcataaa agattagtaa catcagtaat gacagtgatt ttggctttaa tcagctcaat
    12601 aggcacgttc gttttatcaa ctgtagcaat tttacctaga ttttctccca gtgcttctac
    12661 tttttgatta tcttgatcgt aaaaagcata acctagacct tctgccatgc cgatgccgcc
    12721 atcgttggta ctacttccgc caacgccgat gatgatgtgt cgaacacctt gttgcaacaa
    12781 ttgtcgtatc agttctccta ctcctcgcgt ggataaccta agtggctgtt gttggtttag
    12841 tgggacatct ttcaatccga cgatttctgc catttcaaaa atcgcagttt gaccgtctga
    12901 agcgtactgg atttctttgt ttttgccaaa tgcattagta acgactgctg tttgccgctt
    12961 caaccttaaa ctgtctgcta atgatgaaac cgttccttct cctccatcgc ctataggtag
    13021 cagatcatat tcagcctgaa caaaaacttc ttgaaaacct gattgaatgg cttgtgctgc
    13081 ctctaaggct gtcagacttt ctttgaaaga atctggcgca atcagaatac gcattatttt
    13141 cccctccgtt ccaagatcag ttctttttcc agctggtatt ggtctccatt gacaaaaata
    13201 ggtaatgttt ctttcgtctc tttttttatt actatttgtt tttttgtcaa aacaaatagc
    13261 aacctgtgat ttttccaaat aaaaggaaaa gaaatttttt cccatgctga aggaagtctt
    13321 ggagaaatag aaagagttcc attcgttact gtaatacctg aaaaaccaaa tataactgct
    13381 agccatagtg ctcctaatga agcagcatga attccgtcat ctgatgaatg cggatctggt
    13441 cctaaatcaa tcaggcacgc ttcttggaat aaatgatacg cccattccat ttcttggata
    13501 cggcaagcct ctatggcgtg aatcgcttta ctcaacgatg agtcgtggat tgttttgttt
    13561 tcatagtaat gcaaattctt agaaacgatt ggttgagaaa aatactcagg taataagtat
    13621 aacagcatga caagatctgc ttgcttgctg acttggagtt catttacttc gctacgggca
    13681 taatcaagta aaatagactg gctgccagga cgttctttgt aagtggtcaa atcaataact
    13741 ggcttactga gatacgtgtc atcttgcgga atcagctgtt ctttgttagg cactggcaaa
    13801 tataacttat ctaaaaaatt ttgacattct ttcaaaaaaa gcggatcgcc agcttgatat
    13861 ttaacagcta aggccacatt ataatgcgcc agatagttcg tatatgcatt attgtcgata
    13921 tattctgtgt attcatctgg accaatgact ctgtgtatct ctaaatgatc atcgactttt
    13981 acagctctgc tcagccaaaa agctgctgtt tcatgaatga gcgtttttcc ctttgtttct
    14041 aaaaacgatt gatcctctgt tgcttggaca tactcttgaa cggcataagc aaggtctgca
    14101 ctgatatgat gttctgcggc agctgaagca acttcttgtc ttgttccggt tttgatattc
    14161 aatgcagcaa atttgggtgt ctcttcctga ccagtgtgtg cgctttccca tgggaaaagt
    14221 gcaccttgat agtgataatc tcttgctttt tgccgtgcgc cttctaatcg atcataacgg
    14281 tacagcagca aattctttgc tacttcagga aactgatata aaaagaaagg caggataaaa
    14341 atttccgtat cccagaaaac atgcccttta taaccttcgc cagttagtcc cttcgcccct
    14401 acactaacac ttggatcatt gcggggagtc ataatctgta aatggtaagc agcgaagtcc
    14461 atcgctaact gatcgaaaga agaatcgcct tggatcgtta tccgatgctg atgccaaaaa
    14521 tctgtccaat aagacatcga ctgctctaaa atttctgtat aactaacgct tttctgggct
    14581 tgggcaatac ttttcttaaa cgggtctgtt tgctgaagag aggtagagag cacgatgatc
    14641 ttctccagcg taaaggacgt attttttctg atcttttctg aaaaggaagc ggtgatccgg
    14701 cgattttttg cttggaatac acctgcttta ttgaatttag ctgcaatccc aatatcaatt
    14761 ttactttctg ttgtctggta ggatgcactg atcaaatcgt caccaaatac tcgcagttct
    14821 ttttcaacca actgctgtgt accaaaattg gtttgctgtc catcgatacc cgttttcaca
    14881 tctacttggc agtcttcggt caacggcttg atgaccactt tcagggctgc ctgatggaga
    14941 ttattttgat cagcaacacg acaagtaatg acttccactt tttttccttg agcagtttca
    15001 atgagcgcat agcgtttaag ctctcccatt tctaaatcta gtgcccgctc gtatgcaaga
    15061 actttcatcg tctcaagaga aaaatgttca ccgtcgattt ttatttccca tctgatgaca
    15121 tctgggagat tcactaactc ggctgattct gctcctaaag gtcggttata tacaccagct
    15181 gcaaacattc cccttacttg attccgatag tcttcctcct ggcttcctct gataccaaaa
    15241 aatccatttc caatggtcat ctgactcgca tacttattga tatactctaa atcaaactgt
    15301 ttttcttcta ctttcataag gtaataactt ctcctgtttc tgccgaacga tataaatttt
    15361 ccaccagatt ctggatgaca tatccttgcc agccatctgc aatcatctga gtctggtcac
    15421 cgagcgcccg attcacaaaa tgagccatac ttagttcatg tgggtcatta actggcgtgg
    15481 tcttttgtaa ggaaaccagc tttccatcta catcttcgaa tagttcgcct ggatacagat
    15541 tagcacctgc ctgatcgcca caaaaagcca cattcagttt gtcttctctg atattcaaag
    15601 caaacgaagt attcagttgg atgatcttgc cgttttctaa ttccagtgtg ccaaaaattg
    15661 catcttctac tgtgaatttg cttggatccc atttcccgaa tttccctgaa ttttttttcg
    15721 taccaatttt ttggaagctg tacgcccgaa cgctttttac tttaggaaat ccagtaatat
    15781 acatcgctgt atcaagcatg tgaatgccat aatcgatcaa aggcccgcct ccttgaagtt
    15841 ctttgtcgat aaacgttccc cagcctggaa caccagaccg acgaagcgca ttcgcttcta
    15901 cgtaataaat atccccgaga acatcaagat gattcttgat cattgttgct tcgtcagaaa
    15961 aacgatgctg gaatccataa gccaaaatct gcccgttttt atcggctgtc tctgccatct
    16021 ttcttgcttg atcagctgtc atagctggtg gtttctcgca catcactgaa gcattagctt
    16081 cgagggcagc caatacatgt tcgaagtgaa agcgattggg tgtacagaca ctcacgatat
    16141 cgggagaaat ctcattcagc atttgtgtaa ctgatgtata cgcatgagaa aatcctgtac
    16201 gtcggcaaaa gtcttctgct cgctggctga ctggatcaac cacagcggcc aattctaaga
    16261 catctgatct tttttgataa taagcagcat gtactttttc tgctacttgt ccagcgccta
    16321 tgattgctac tttcttcatt tatttctccc tcctttgctt atttacttat tgttattctt
    16381 tctctattct ttctctaaat catttaaatg acgcgctgtt tccagacagt ttttcaagta
    16441 ttgcaatgat tcctgataaa ttttcacttc attttctcct cgcaaacgac attcatatac
    16501 catataacct tcatactcat tttgaagtag cgtttgaaaa tggcgagtaa aatcaattgc
    16561 gccagatcct ggttggtagc gatgattatc tgctaaatgg atatgaccca catactcttt
    16621 ttgcgtagtc aatgcttttg aaatatcatc ttcttcaata ttcatatgat aaaaatcagc
    16681 gatgatctgc acatgtttca attgattaga gtcaatgtaa tgacgcgcat ccgctaacaa
    16741 attgatcata tggtcttggt atcggttcaa aggctccaaa taaataaccg tacctgtttc
    16801 ttcggctact ttatctaaat aacgaagtga ttcgctgact gctttatagt ctccttcttt
    16861 gcttcggggt gaaaccatcg gcggtaatcg ataagtgaac attccccaag ctgctgggat
    16921 aacgattcct ttgcctccaa cctcgcttag tgcacgcaaa atctcagcaa tttgacgtac
    16981 accattaaga cgccgttctt cgataaagtc accgatccac ccatcgtagc cgttacaagc
    17041 tgtcactact gggatccctg tttctttgat tgcctgtttg actgtgtcga tattttctat
    17101 taacaactta ccgtcgattt cgtattcttc aaatccacag tctttgataa atctaaattt
    17161 ttctaaaatg tctcttggga aaaatgcttg atcttgcgta ccgaatttca ttgttctcct
    17221 cctcggactt gaacgcccat tttgatactt tcttctgggt gctggtctac ataacgcatg
    17281 taagcttctg cgctttctgc aaatggcaca actggatcaa tgatttcttc acaatccaag
    17341 taaccgttca tcaataattc ccaacatgtg tcctcgattc gtttacggtt ccatcttggg
    17401 taatctgggt ttggttcact agatgctcga gcaaagacga tttttgcatt attgaaatgt
    17461 gcttcgcgac caagattaaa cccagctgga aaaggtttag caaaggcaac ataagaaatg
    17521 atcccgccat agccgatccc tcttaaagaa gcttgtaatg cggcagcact gccactcgtt
    17581 tcaataactg aatctactcc acgtttatct gtcaattcct ttagacgaaa accgacatct
    17641 gctttcatag gatctagcgt gtaatcggca ccgtattttt ctgctaattc tctacgctgc
    17701 gctatgggat cgatcccaac gatggtagta gcacctgctt ttttagctaa ttgaatcgca
    17761 atttgcccga tagcgcctaa accgaagatt gctacagcat cacctgctcg aacatgtgct
    17821 tcccggatac cacctaaagc gaattgagca ggatcgtaac atactgcatt tttccattga
    17881 tccaaccgat caagtttacg tagtttataa ttatcaacac cgtttgtaat cagcgtttca
    17941 cgaatgggtc cataagtgca gactaactct cctttttgat actctgtcac caaactgccc
    18001 gtttcttcga tcgtaccgac aatcatattt cctaattgaa attcgccaaa taccacaccg
    18061 cgttggcttc cttcttctct tggcaagaat aggcgccatt cttctgaaaa ttcttcatcg
    18121 ataaatggac ttaatcctct aaagtcgaca atttctgttc ctggttttgg agtagcgaaa
    18181 attactcgga tacgtacttc gttcttttcg atctcacggt cttcgtaatc tttgaatccg
    18241 gctattcttg gttctaatgc aactaatttt ttcatctttt ctttcctctt tcttttcttt
    18301 ttatcctttt acgccgcctt cgactaaatc acctttaata aatcgttcag ataacgcata
    18361 cataatgatg actggtaaag cggtcaccaa tgaagcggcc atcattcgtc cccaaatgta
    18421 gtctggtgta ctgaataatg tgttcaaacc gattggtaaa gtaaattttt cggaggtcga
    18481 caggaaaatc gaagcaaata aataatcatt ccacgaaacc ataaagcaat aaacgaagac
    18541 agaaatgatt ccagaaatag ccaatggtat aatgatccga ataatgatct ggatccggct
    18601 aagaccatcg atcattgctg cttcttcaat atcagtcgga atcgtgtcaa aataactttt
    18661 tagcatgaat atcgcagtag gaagtgtttg aacgaccatc gtgataatca gtgctgtatt
    18721 ggtatcatac aaacctagac cggaaatgat tttaaataat ggaacaacga tcaaaatacc
    18781 ggaaaacata taaacggtgt aaaagctagc attgattttc gagcgagctt tgaagcgtag
    18841 ttttgacaaa gcgtatgccc cgctgatccc aatcactact gcagttacgg aggctatcaa
    18901 ggagatcact aaactatttt tgaaatacat gatgaatgga aaaatctcag cattgaaaat
    18961 atcctgataa tgttcaaaag tccattcttt cggaaaaata gtttgtgtcg ttgcaattgc
    19021 ttcttgtgaa tccttgaatg atgtcatcag catgatcaaa aatggaaaca aattgacagc
    19081 taatagcaac aaaatcgtcg cgtaaaataa gattttcctg ctctttttat ttttcatttc
    19141 aagtccacct tctttcgtga gagcaagacg accgctgcaa taatgatgaa caatagaatc
    19201 gatatagctg ctgcttttcc aagatcattg aacgcaaagg ctgtctgata caaataaacg
    19261 cctaagatat tgactttatt cgtcagtaga tagacatctg taaacatata gaacatccag
    19321 atagatcgta aaacaatcac agttgctagt actggtttga ttgccggcag tgtcacgatc
    19381 ttgaacttct gccaagcatt tgcaccgtcc atttcagctg cttcataaag cgttttgtcg
    19441 actgtttgca aaatagccaa aaatgaaata aacgcatacg gatagtatct ccagatagca
    19501 aacaaaacga ccataaagaa gctagcaact ggctggtcga accacagcgg tgcttcgttg
    19561 aaaagctgca acctatctac taacagatca ttgacgattc cataactatt attgaacata
    19621 tatttccaag caaaaatcaa agaaatagat ggcgtaacat aacttaataa aatcagtgaa
    19681 cgcaccgttt tcctaaatct gaacttgcgg ttaagcaaaa tagctacggc taatcctaaa
    19741 gctgtactgc ctgctacaac taagaatgtg taagcaactg ttaaacctaa agaacgataa
    19801 aattcactat cacttaaaat ttcaagataa ttgcttaatc cgataaaaac ggcgtctgag
    19861 ttaggattca gcggcatatc gaaaaaactg atctgaatat tggaaagcat tggaatcagc
    19921 actaaaacag ctaataaaag catgcttggt gctaaaagtc ccatggctaa ttttaagtca
    19981 gaatgtttcc ttggacgatt cattgaaacc tccccctgat aatctgccaa tagctatcta
    20041 cttttttcga acaccattgg cagaacttct tgttatttcg tatttttttg tgcgtcagct
    20101 aaagcttgat cgacatcttc gtttccgaca gttacttggt tcactgcttt tccgatcaca
    20161 cctgagcttg tgatatctcc cattttcgtg aaattctttt ccccgactag tccaaagact
    20221 tgtacttcat caaaagcact agcaatctca gatggcagct cgccaaaaga cttgatgact
    20281 tcattttctt tgtaaccatc cagttcgact acttgtttat tcactggttg agcaccacca
    20341 ggagacatca agacccactt ttccatgttt ttcggttgag ataagaaagc tgtgaatgct
    20401 tcagcagcct ctttttgttt ctcgtctaga ccagaagaaa tcgtcaatcc agatactgtg
    20461 ccatagacag cttgatcttt ttgtgttggg atagcaaaac cgatattttc tgagttcccc
    20521 gcttcataaa cagaaggcaa aatgtaagtt gaatagattg ccattggtgc tgttccattc
    20581 atgaaagcat ctttgatttc tgtcacatca ttagagcctg gcatcgtgta ttgggataag
    20641 ttttgataaa acgacaatgc ttctctcatc ttttccgtat cgatcgttac ctcgccttca
    20701 tcatccaaaa catttgctcc atttgacaaa gcaaattgag agaacgcttg ctcactcatt
    20761 gttccttcag cagtaggcat agcaattcca tattgtttat tctctttatc tgtgaaatgt
    20821 tgagcaattt ttaaaatatc atcccaattt tctggttctg aaaaaccagc ttctgataat
    20881 ttttctttgt cataccaaat accttgaacc cagccgctaa ttggtgcagc aatatagctt
    20941 tttccatctt ctgaacgaac aaggtttttg gctccgtcat aataattatc ttctccagct
    21001 tcctcgataa cgttttggac tgccgtctga tcaatcagtt catctttatc catcacttta
    21061 gcaaaatcct gactgacttc cattacttct ggtaatttac cggaacgagc taacgtaacg
    21121 atttttgtat tgaacgctga ttcttctacc ggcacttgct tgattttgat gtctggattt
    21181 tctttttcaa aatcagcaac cagttcattg atcacactta atcgttcctg ttcgacagaa
    21241 gagtgcatga attgaatcgt tgtctgttgt gattcgttag agctttgact ttcaccacaa
    21301 gcagtaagaa gaaatccagt agctagcaga caggtagtaa caataatttt tttcattttt
    21361 ttccacctct tattttatta ttttcagcca catatattca tacggctgga atatcaactt
    21421 tcgttcttga ctgcaatgga taatttgtcc agaaaacagg tctaggtaat ctccttttgg
    21481 taaaagataa cctgctgatt gattggaaaa attatggata actaagactt tctcttcctt
    21541 cgcttcgcga ataaaactga taaaagaatc tgctgagtcc agtaagtaaa tcaaactatt
    21601 cggatggaag gctttttctt tttttcgaat agatagcatc tgggtcaatt caccatatac
    21661 tgctgttctt aatggatctg tctgcaactc tttctcaata tctgctaaca aaaatttttg
    21721 ccgattgatt tttcgattct ctcctgtttt ttctacgcct ttgtaatcat ttcgacttcc
    21781 cagcacactt tgaatataaa ttgccggcac gcctggtaac gttaccaaaa atgaatgagc
    21841 agctaaaaag cgtttgcttc tccattccgt cgtatctgtt ggagaactta acgcatctag
    21901 atacgtagcg tttatctcat atggactggt cgttccatct gggtttttct tgtagtttac
    21961 tcgggtaccc tctttcttca gctgttccac taattcaagg atcgtttttt ccggcagaat
    22021 acctcgtacc ggattcaatc cgataccgtc atgggaggct aaaaaattaa aaaaagttgt
    22081 ttttttcgat gcgaaatcga tggtttttaa ccattctttc aacttacttg tattacccga
    22141 acgaatcgct tgaagggtca aaggtggtag cgaaaactga tagaccatct gtgcttcatt
    22201 ctcgccattc ccaaaataac tgatattatc tttgtgtggc acatttgtct cagtgatcaa
    22261 taccgttcct ggagctgctt catcagcgat tgcacggaat aattggatga ttttatgcgt
    22321 ttctggcagg tgtatacaag aagttcctag ttctttccat aaaaatccca cagcgtccaa
    22381 gcgaataaat gaagcgcctt tttctaagta aaacaaaaga atatcgatca ttttcaataa
    22441 aacttctgga tttttaaaat tcaagtctac ttgatcttcg ctaaaagtcg tccatacgtg
    22501 tttttctttc ccgtttttgt caatgaacgt tgtaagtaac gggctggtcc ttggtctagt
    22561 cacgctgctt gtatctgtat ccagtggcaa atcaataaaa aaatctttgt acgtatcatt
    22621 caagtttaaa tattcctgaa accaagtact ttcggcagac atatgattgc agacaaagtc
    22681 gaacattaac cgtgtttcgc ttctcatttc ttctatatct tcccatgtgc ccaaacggtc
    22741 gttgattgcc tgataattta caacggagaa accatcatca gatgtatatg ggaaaaatgg
    22801 caagaaatgt acaactctaa atgtatctgc caaatacttt tgataaaatt tcttaaatgt
    22861 acgcaaagtt ggttgctgct cttcaaaaaa ttgatcccca taagtaatca agaaaatatc
    22921 ttcttcgtcc caatgcagtt tgcctgcctg gcctttcttc tgctgataaa cactgacttt
    22981 ttgttcaatc atagaggcgg ttttctcaga caaatctgag tcgcgataaa tcgcttgtaa
    23041 aagttttgta tacatcggat tcccctttct tttttgggag cgttcccaac gatttgatca
    23101 tattatggaa aacgcttact gtcaatagca tttttttaaa taattttgat atacacaaaa
    23161 atacaaataa gaaagcttat gtatcaataa ttaatagctt cttaaaaaaa tatggtctct
    23221 catgatcatg caagctattc ctaaattttc aaaatcatag acaaaaaaga atgtctattg
    23281 tactatggaa ttattctaaa acgctcccaa aaaggaggtg catataaaat tgaaacctac
    23341 tatttatgac attgcgaaaa aagctaatgt ctcaaaatca actgtctcca gagtattaaa
    23401 taaccagcca aatatctctg ataaagcaaa agcaagtgta cttcaagcaa tcaaagaatt
    23461 gaattatgaa ccgagcaaaa ttgcaagagg actgtcagga aatggttttg acgcgattct
    23521 agcattatct catcgtactt ctcatacgac ctatggcaat cctttttttt cagatgttat
    23581 tcaatccatt tctgccgttt ctgaacaaaa taacttcgac ttgattctac aaacggcaaa
    23641 aacaccagaa gaagagctag aaaaatgcgc gaaaaagatc caagaaaaaa tgatccgagg
    23701 aattattatt ttaagctcta cgatggatga aacacttctt tttgaattgg atcaatacaa
    23761 tattcctatt gtggtaatcg gaaaagtcgg aaacgactac acgaacatct actcagtgga
    23821 cacggataat ttccaggata gttacgattt ggtggaaaca ctgatcaaga atggacaccg
    23881 tcatatcggc tgtctccatg ctccgacaga agtttatgta tccaatgatc gtctcaatgg
    23941 ttaccgtcaa tgcttatttg atcaccacct agatatccgt aatgaatgga ttatagacgg
    24001 cggctttcgc ttagaagata gcatggcagc aatgaatgat ttaatgaagc tacgagaaag
    24061 accaactgcg attttttcta ccgatgcatt aaaatcattg agtatttaca aagtggcaga
    24121 acaatctgga aagcagatac ctgatgatct ttcgatcgca ggattcagtg atcccttatt
    24181 gtcctcccta ttttcgccac agttgactcg gatagacatt ccgacaaatc agttaggaat
    24241 caaagccaca gaacttttat tccaactaat ccaccaccaa taccctgaag aaaaaagaat
    24301 cattctttca accgagatta ctctcacaga ttcgatccaa caacaagaaa tgtagaacag
    24361 aattataaaa gaacagaaac actgaggata agtcagttac tctgttcttt tttcaatcac
    24421 tcatccctcg ttaccccctc ccggtatttt agcgaatttt tcttattttc catctgtttt
    24481 agatagctgt ggataactat aatgtttgtg ttaaactatt ttttccacag ggaagaaaaa
    24541 actattttta accacgttgt gtataagttt ttatagttcg tttttttaaa cacatttacc
    24601 cttatttcaa tccatgttat ttggggatac ttatctctaa aacataaata gaataacaga
    24661 catgtctact ggttttgttt tattcgagga attattcaat aatagctagc aaaatattaa
    24721 aaataaccgc tctatcttcc catcaattta ttttgcattc aaaaaatgat ctatctttac
    24781 ttttgttgat tgacttctct tggaagatga tcgttccgat accttaattg gatacgaacc
    24841 gatgttgtta agatcctttc ccacttttct tctagttaga caaattattt tagac
//
LOCUS       DASJSZ010000038        24321 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.038, whole genome shotgun sequence.
ACCESSION   DASJSZ010000038 DASJSZ010000000
VERSION     DASJSZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.038"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..1720
                     /locus_tag="U4B03_001427"
     CDS             59..1720
                     /locus_tag="U4B03_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2068286.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1790..2152)
                     /locus_tag="U4B03_001428"
     CDS             complement(1790..2152)
                     /locus_tag="U4B03_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEN2068287.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2220..3161
                     /locus_tag="U4B03_001429"
     CDS             2220..3161
                     /locus_tag="U4B03_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068288.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3162..4205
                     /locus_tag="U4B03_001430"
     CDS             3162..4205
                     /locus_tag="U4B03_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068289.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4211..5281
                     /locus_tag="U4B03_001431"
     CDS             4211..5281
                     /locus_tag="U4B03_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068290.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5271..6224
                     /locus_tag="U4B03_001432"
     CDS             5271..6224
                     /locus_tag="U4B03_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2068291.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6236..7246
                     /locus_tag="U4B03_001433"
     CDS             6236..7246
                     /locus_tag="U4B03_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2068292.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7531..8352)
                     /locus_tag="U4B03_001434"
     CDS             complement(7531..8352)
                     /locus_tag="U4B03_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2068293.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8449..8727)
                     /locus_tag="U4B03_001435"
     CDS             complement(8449..8727)
                     /locus_tag="U4B03_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068294.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8995..11004
                     /gene="metG"
                     /locus_tag="U4B03_001436"
     CDS             8995..11004
                     /gene="metG"
                     /locus_tag="U4B03_001436"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEN2068295.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11483..12070)
                     /locus_tag="U4B03_001437"
     CDS             complement(11483..12070)
                     /locus_tag="U4B03_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068296.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12245..13567
                     /locus_tag="U4B03_001438"
     CDS             12245..13567
                     /locus_tag="U4B03_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2068297.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13722..14498
                     /locus_tag="U4B03_001439"
     CDS             13722..14498
                     /locus_tag="U4B03_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN2068298.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14491..15060
                     /gene="rnmV"
                     /locus_tag="U4B03_001440"
     CDS             14491..15060
                     /gene="rnmV"
                     /locus_tag="U4B03_001440"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEN2068299.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15053..15937
                     /gene="rsmA"
                     /locus_tag="U4B03_001441"
     CDS             15053..15937
                     /gene="rsmA"
                     /locus_tag="U4B03_001441"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEN2068300.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16388..17017
                     /locus_tag="U4B03_001442"
     CDS             16388..17017
                     /locus_tag="U4B03_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068301.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17141..18250)
                     /gene="ugpC"
                     /locus_tag="U4B03_001443"
     CDS             complement(17141..18250)
                     /gene="ugpC"
                     /locus_tag="U4B03_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEN2068302.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18459..18881
                     /gene="mgsA"
                     /locus_tag="U4B03_001444"
     CDS             18459..18881
                     /gene="mgsA"
                     /locus_tag="U4B03_001444"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEN2068303.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19204..19740
                     /locus_tag="U4B03_001445"
     CDS             19204..19740
                     /locus_tag="U4B03_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN2068304.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19780..21543)
                     /locus_tag="U4B03_001446"
     CDS             complement(19780..21543)
                     /locus_tag="U4B03_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068305.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21546..23261)
                     /locus_tag="U4B03_001447"
     CDS             complement(21546..23261)
                     /locus_tag="U4B03_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068306.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24040..24264)
                     /locus_tag="U4B03_001448"
     CDS             complement(24040..24264)
                     /locus_tag="U4B03_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068307.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         7868 a         4418 c         4920 g         7115 t
ORIGIN      
        1 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
       61 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      121 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      181 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      241 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      301 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      361 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      421 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      481 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      541 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      601 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      661 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      721 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
      781 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
      841 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
      901 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
      961 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1021 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1081 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1141 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1201 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1261 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1321 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1381 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1441 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1501 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1561 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1621 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1681 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1741 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     1801 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     1861 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     1921 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     1981 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2041 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2101 gaaaagctac tgcgtagggg atagattttt tttcatttga ttgctccttt atttttggtt
     2161 cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg gaaacattag
     2221 tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc acgctttggt
     2281 taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca tatacgaatc
     2341 aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt ttggataaac
     2401 cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat tttggaattt
     2461 ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc ggaccatcct
     2521 tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt ttaggaacga
     2581 tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta gcgatattag
     2641 gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt gcaatcaaat
     2701 tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta ccaacaattg
     2761 cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa atggtggaag
     2821 tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt tgggaaatcg
     2881 cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta ggaccgttag
     2941 ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt cctggtatcg
     3001 gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg gctgtaacga
     3061 tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg tacggtctag
     3121 ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg ataccaaagc
     3181 gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa aaaaataccg
     3241 aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat tcatggcgcc
     3301 gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc atcattttga
     3361 tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac gtggcttata
     3421 tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga aaagcaatgg
     3481 ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac gtaaatttct
     3541 ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg ggaatgagaa
     3601 tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt gtagcttacg
     3661 ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa
     3721 tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt tttgaaccag
     3781 gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg gcacgtatcg
     3841 ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga ttgacattag
     3901 gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct agtgtcatta
     3961 ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt ttaagcttta
     4021 tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc gaagggtaca
     4081 agacattcct gtatctgcct tatctattat ggattccggc agtaacttta tcggttatca
     4141 tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag
     4201 agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt ttgatacgta
     4261 tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag gagaaacatt
     4321 ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca tcatgcgctt
     4381 gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta acgatatcgt
     4441 tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca
     4501 agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg ctgagtccct
     4561 tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag aattgcttaa
     4621 attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc agttctcagg
     4681 aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg aaatcctgat
     4741 tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt tggagctgct
     4801 gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg accttggtgt
     4861 agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg
     4921 aacatcggaa gaaattttct acaacccgca acatccctat acttggggac tgcttggttc
     4981 aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt ctccaccgga
     5041 tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg cgatgaaaat
     5101 cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg cagcaacttg
     5161 gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac gtcgctgggc
     5221 tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg atgccaaata
     5281 aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac
     5341 atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt ttagttggag
     5401 aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat gaacctactg
     5461 atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa aaaggtttga
     5521 cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg ttgaatcctc
     5581 gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta gctaaatcat
     5641 ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg aatccaagcc
     5701 acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt ggtattgcac
     5761 gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct gcactagatg
     5821 tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa catcaattga
     5881 cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac cgaatcggtg
     5941 taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat aatcacggtg
     6001 ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca gaccatgaga
     6061 gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta cgtcaacttc
     6121 gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca tattacgcaa
     6181 aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga catagatgct
     6241 aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat tgattcgtac
     6301 ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga aaagttggca
     6361 acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag aatacccatt
     6421 cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg atcgggcttt
     6481 tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg
     6541 gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg gctacttaaa
     6601 gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg aaggatcgat
     6661 tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac tactttgtga
     6721 tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct atcatccgaa
     6781 gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc accagacaga
     6841 agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag
     6901 aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag taatggatct
     6961 ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg ggaaattggt
     7021 caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac atgctgattt
     7081 ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc aatggtataa
     7141 taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg taagaaagag
     7201 ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa tgaatgaaac
     7261 tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt tgtttctagg
     7321 aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca atagcagaaa
     7381 aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca
     7441 tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa
     7501 tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata
     7561 gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag
     7621 catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc
     7681 aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg
     7741 ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc
     7801 aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac
     7861 ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc
     7921 cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt
     7981 gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt
     8041 ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt
     8101 tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc
     8161 ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac
     8221 gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt
     8281 aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt ctttagaagc
     8341 aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca
     8401 gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa
     8461 aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata
     8521 ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg
     8581 caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata
     8641 catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag
     8701 tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc
     8761 tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa
     8821 aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat
     8881 agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat
     8941 ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca
     9001 gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc
     9061 ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc
     9121 tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca
     9181 gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa
     9241 ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat
     9301 aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt
     9361 gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca
     9421 gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa
     9481 gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta
     9541 ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg
     9601 atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact
     9661 tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta
     9721 tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac
     9781 tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg
     9841 ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg
     9901 ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg
     9961 cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc tgttccgttt
    10021 gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta tgatctggca
    10081 aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga
    10141 ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct
    10201 gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg
    10261 gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg
    10321 gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct
    10381 gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag
    10441 atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc
    10501 gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat
    10561 atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa
    10621 gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc
    10681 aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa
    10741 gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt
    10801 caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa
    10861 gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg
    10921 atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg
    10981 ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac
    11041 cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca
    11101 atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc
    11161 ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac agaaaaaaag
    11221 cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt ttttattgtc
    11281 cttacattca taaagtttga atgcaatgac tttattacat ctatatttat atctaacagt
    11341 agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat
    11401 atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt
    11461 tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca
    11521 aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa
    11581 tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca
    11641 accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg acttcactaa
    11701 gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt
    11761 tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag
    11821 gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt
    11881 tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg
    11941 caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt
    12001 ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct
    12061 taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa
    12121 aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt
    12181 aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat
    12241 gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat
    12301 gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca
    12361 gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg
    12421 attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat
    12481 tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta
    12541 ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt
    12601 acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg
    12661 gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa
    12721 attaatacca attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt
    12781 ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc
    12841 gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga
    12901 aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac
    12961 agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc
    13021 cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa
    13081 gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac
    13141 tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct
    13201 gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt
    13261 agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt
    13321 gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc
    13381 ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct
    13441 cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg
    13501 tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga
    13561 agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt
    13621 gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta
    13681 gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata
    13741 cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag
    13801 aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt
    13861 tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg
    13921 ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg
    13981 ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc
    14041 aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta
    14101 ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag
    14161 atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg
    14221 acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc
    14281 aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc
    14341 tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag
    14401 aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa
    14461 atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca agagattata
    14521 gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata
    14581 gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag
    14641 actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc
    14701 attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca
    14761 aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg
    14821 aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac
    14881 cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt
    14941 gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt
    15001 cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga
    15061 atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc
    15121 atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt
    15181 agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc
    15241 attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg
    15301 tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca
    15361 agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc
    15421 gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct
    15481 tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg
    15541 tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta
    15601 tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt
    15661 agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga
    15721 aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa
    15781 taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt
    15841 ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc
    15901 cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt
    15961 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt
    16021 ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
    16081 ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc
    16141 atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact attatgccga
    16201 acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat
    16261 tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac
    16321 agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag
    16381 agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat
    16441 gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt
    16501 ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt
    16561 tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc
    16621 attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat
    16681 cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt
    16741 gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg
    16801 taatatgcct gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg
    16861 gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac
    16921 actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga
    16981 gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac
    17041 tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc
    17101 tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt
    17161 gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt
    17221 cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt
    17281 ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta
    17341 cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac
    17401 cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag
    17461 gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa
    17521 agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat
    17581 aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt
    17641 cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac
    17701 gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg
    17761 ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca
    17821 cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac
    17881 ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta
    17941 atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt
    18001 ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga
    18061 tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact
    18121 ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa
    18181 aatcagttac agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca
    18241 tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa
    18301 atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca
    18361 atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat acactggagt
    18421 aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc
    18481 atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa
    18541 aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc
    18601 cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt
    18661 cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg
    18721 aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga
    18781 atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa
    18841 gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa
    18901 ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga
    18961 tataatacga acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa
    19021 atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa
    19081 ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc
    19141 gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta
    19201 aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca
    19261 ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc
    19321 acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc
    19381 gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga
    19441 tttacattga atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag
    19501 atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt
    19561 ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg
    19621 gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac
    19681 gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa
    19741 aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg tgaattgtgc
    19801 ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg tgcccatttc
    19861 gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg tggacaggcg
    19921 atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt gttggatttt
    19981 tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca tagggggatt
    20041 actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata tctttaaccc
    20101 ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt catacatata
    20161 agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc caaaacgaag
    20221 attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc caaaactttt
    20281 acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa ttgttccttt
    20341 tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg cccctgtttt
    20401 tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt cgatcaaacg
    20461 ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt tgatattttt
    20521 gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat cgatcaaatc
    20581 aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc cagaaagttc
    20641 gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca caccaacagt
    20701 gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg ataaataagc
    20761 aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt gtgcccgttg
    20821 tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag cttcttcttg
    20881 ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc ccgagatatc
    20941 cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa cgatccaact
    21001 cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg gacttagacg
    21061 catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt gattgaaggc
    21121 cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga cgatatttcc
    21181 gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa atgcatcttt
    21241 tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga tgagccattg
    21301 actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga acagtcgatt
    21361 gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt gtaacgtcat
    21421 cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa tgtcttttgc
    21481 ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt tttttacttt
    21541 cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga ttgagcaagt
    21601 aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa gatatgatgt
    21661 gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc aagataagtc
    21721 tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa agagtcatct
    21781 aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg ctgtttttgt
    21841 ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt tggaagctga
    21901 cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt ttctgtggcg
    21961 tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac agaagtttgg
    22021 ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac atccattccg
    22081 tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg tgtcaaagtc
    22141 gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt cagctgaaat
    22201 gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac atggtcaaaa
    22261 gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc aagaatcgaa
    22321 ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg tgtgaagatc
    22381 acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata attgatcaaa
    22441 gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc aactgtcagc
    22501 aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag ggcagagata
    22561 ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa cttctctgtt
    22621 tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt ctgactaacg
    22681 tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac ggtttttttg
    22741 atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa cagacctact
    22801 tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg tgctctgatc
    22861 aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt tgtcattcgg
    22921 gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt attgattttt
    22981 ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga agcatagtat
    23041 tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca taagcccatt
    23101 tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga agccatcatt
    23161 aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc caaaatgatt
    23221 tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct cctattattt
    23281 atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa tttagaatga
    23341 aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt agaaattgaa
    23401 tagactattt tttttaaaaa aaagaaaatg agaaaacatg ccagtctctt cattcatagg
    23461 aacgtctatt ttacttaatt tttctataaa tgaattctgc ataaaaaata tatcaagtaa
    23521 atgtttatag atgaaactct atttacttct tttttaattt gttcttgcaa tcttttttgc
    23581 tacgtaattt ttgtaaaaaa gtgggttgag agaaatctaa ttgattttca aagaaggagt
    23641 atgaaaatga gaaaaatatt tattcgttta ttgtgggtac tatcttagag gaataaataa
    23701 tagtggagaa aactatggtt tggcgcagat tctatcaggt ttgatggaac gaagacaacc
    23761 tcccgacatc aagtcaaaag ccacttgaga ttttgatctg aaaataaaaa aagaaaacaa
    23821 ggtaggaaca tcaatgtttc taccttgttt tcgtaatcaa tgggaaaaaa ctaagagtgg
    23881 tgtacgtaat caataaaaag gatggtgaca acagtacctc tttactctct gaaagcatca
    23941 taactcgttt ttcctcatta tgccaataaa ttgttgttat accacatata aggatttgta
    24001 atagctaaaa acagacagga aagttgaaat ctgtgaatct tattttgtat ggaaggattt
    24061 atagaacgca aaaatcaaga taaagatgaa taaaaatcct gtttcggcta tatattgcgt
    24121 atctacgcca ataaaacgag tttgttctgt catagccata taaagcaaag gcaaaaatac
    24181 aactacagcg agataagatc cgatgatttc tcgaaaagtt ttccaggaag tatgtttcct
    24241 gtttttatat ttatccgatt tcattgaata gtcatagcct ccttattctt tcatatgttg
    24301 aaattccagt ataaaaactg g
//
LOCUS       DASJSZ010000039        24260 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.039, whole genome shotgun sequence.
ACCESSION   DASJSZ010000039 DASJSZ010000000
VERSION     DASJSZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.039"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="U4B03_001449"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="U4B03_001449"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEN2068308.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="U4B03_001450"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="U4B03_001450"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEN2068309.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="U4B03_001451"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="U4B03_001451"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEN2068310.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="U4B03_001452"
     CDS             complement(3373..4680)
                     /locus_tag="U4B03_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEN2068311.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="U4B03_001453"
     CDS             complement(4683..5264)
                     /locus_tag="U4B03_001453"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEN2068312.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="U4B03_001454"
     CDS             complement(5836..7128)
                     /locus_tag="U4B03_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2068313.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="U4B03_001455"
     CDS             complement(7125..7748)
                     /locus_tag="U4B03_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068314.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="U4B03_001456"
     CDS             complement(7751..8587)
                     /locus_tag="U4B03_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2068315.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="U4B03_001457"
     CDS             complement(8604..9491)
                     /locus_tag="U4B03_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2068316.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="U4B03_001458"
     CDS             complement(9507..10790)
                     /locus_tag="U4B03_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2068317.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="U4B03_001459"
     CDS             complement(11473..12156)
                     /locus_tag="U4B03_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN2068318.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="U4B03_001460"
     CDS             complement(12174..12791)
                     /locus_tag="U4B03_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2068319.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="U4B03_001461"
     CDS             complement(12801..13625)
                     /locus_tag="U4B03_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN2068320.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="U4B03_001462"
     CDS             complement(13618..14178)
                     /locus_tag="U4B03_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068321.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="U4B03_001463"
     CDS             14352..15308
                     /locus_tag="U4B03_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2068322.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="U4B03_001464"
     CDS             complement(15613..16569)
                     /locus_tag="U4B03_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEN2068323.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="U4B03_001465"
     CDS             16673..17275
                     /locus_tag="U4B03_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2068324.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="U4B03_001466"
     CDS             complement(17363..18862)
                     /locus_tag="U4B03_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEN2068325.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="U4B03_001467"
     CDS             complement(18878..19402)
                     /locus_tag="U4B03_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEN2068326.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="U4B03_001468"
     CDS             19700..20449
                     /locus_tag="U4B03_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN2068327.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="U4B03_001469"
     CDS             20440..21225
                     /locus_tag="U4B03_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEN2068328.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="U4B03_001470"
     CDS             21194..21910
                     /locus_tag="U4B03_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2068329.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23046)
                     /locus_tag="U4B03_001471"
     CDS             complement(22012..23046)
                     /locus_tag="U4B03_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEN2068330.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23050..24108)
                     /locus_tag="U4B03_001472"
     CDS             complement(23050..24108)
                     /locus_tag="U4B03_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEN2068331.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7625 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgattttt
//
LOCUS       DASJSZ010000040        24070 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.040, whole genome shotgun sequence.
ACCESSION   DASJSZ010000040 DASJSZ010000000
VERSION     DASJSZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.040"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1273..1938)
                     /locus_tag="U4B03_001473"
     CDS             complement(1273..1938)
                     /locus_tag="U4B03_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2068332.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2118..4100
                     /locus_tag="U4B03_001474"
     CDS             2118..4100
                     /locus_tag="U4B03_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEN2068333.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="U4B03_001475"
     CDS             4104..4742
                     /locus_tag="U4B03_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068334.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="U4B03_001476"
     CDS             4770..5198
                     /locus_tag="U4B03_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068335.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="U4B03_001477"
     CDS             5655..6566
                     /locus_tag="U4B03_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN2068336.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="U4B03_001478"
     CDS             6783..7220
                     /locus_tag="U4B03_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068337.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="U4B03_001479"
     CDS             7315..8199
                     /locus_tag="U4B03_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN2068338.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="U4B03_001480"
     CDS             8423..11590
                     /locus_tag="U4B03_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068339.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11610..12155
                     /locus_tag="U4B03_001481"
     CDS             11610..12155
                     /locus_tag="U4B03_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HEN2068340.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12183..12638
                     /locus_tag="U4B03_001482"
     CDS             12183..12638
                     /locus_tag="U4B03_001482"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HEN2068341.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12635..13393
                     /locus_tag="U4B03_001483"
     CDS             12635..13393
                     /locus_tag="U4B03_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2068342.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13479..14219)
                     /locus_tag="U4B03_001484"
     CDS             complement(13479..14219)
                     /locus_tag="U4B03_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2068343.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14280..15533
                     /locus_tag="U4B03_001485"
     CDS             14280..15533
                     /locus_tag="U4B03_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2068344.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15855..16082
                     /locus_tag="U4B03_001486"
     CDS             15855..16082
                     /locus_tag="U4B03_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN2068345.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16205..16531
                     /locus_tag="U4B03_001487"
     CDS             16205..16531
                     /locus_tag="U4B03_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN2068346.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16599..17312
                     /locus_tag="U4B03_001488"
     CDS             16599..17312
                     /locus_tag="U4B03_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN2068347.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17622..18472
                     /locus_tag="U4B03_001489"
                     /pseudo
     CDS             17622..18472
                     /locus_tag="U4B03_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            18593..20458
                     /locus_tag="U4B03_001490"
     CDS             18593..20458
                     /locus_tag="U4B03_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2068348.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20506..21915
                     /locus_tag="U4B03_001491"
     CDS             20506..21915
                     /locus_tag="U4B03_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEN2068349.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            22023..22724
                     /locus_tag="U4B03_001492"
     CDS             22023..22724
                     /locus_tag="U4B03_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2068350.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22883..23362
                     /locus_tag="U4B03_001493"
                     /pseudo
     CDS             22883..23362
                     /locus_tag="U4B03_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         8104 a         3928 c         4954 g         7084 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaagtaa gcatactttt ctaactctgt cttcaattgt
    13681 tttaaagaag tattcactaa tttatcttct gtagttgttt tgaccataac ttggtttttc
    13741 aaacctttga tactttcaat atataaaatt tcccgaggat acaggacgag acttccacct
    13801 ttttgatcaa taaagatttt atcgacattt acgatctgct gttctttcca attgatctgt
    13861 tctagcactt gacgaatttg ttcagataaa ttcctgtctt ttctgatata tccaatcgga
    13921 aggattttcc gattgatgat ctctactgca aaattcgtat aagcggtgaa aaaaacaata
    13981 ttactatgtg catttttttt acgtatttgt tcggcgacat caatacctgt gagtgattgc
    14041 ttcaagtcga tatcgataaa gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac
    14101 tgattgattt gagagataga agtgagctgc acgatattct cgatatgatg attgaccaat
    14161 tctctgatga tccattcgct gtgaaactgg ttatcttcta tgataaaata ctttttcata
    14221 tgcaggctcc tattaaaaaa atatgtgtac attttgaaac aaaacaatgg aggaataaga
    14281 tggtcgcaac agtgacactt tatttgacct ttactttgat tttagattta ggtatctatt
    14341 tattcttttc gaaacaactg ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg
    14401 tgacactttt acttcctatc cctaaagtga gctttcctta cctagttggc ttattattga
    14461 tgtttctttt ttattttact acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt
    14521 tttttgctat cactggactc agcttttttc tggtttttga tctgatggat tatttaggaa
    14581 agagacatct tggaacagca agtttttttg cactacttat cgcatttatt ttctatctat
    14641 tagtaaaaag tatagacaaa cagacgagat ttctcaactt gctttttact tatccaactg
    14701 aacactattt tggaggtatt tgtattttat taagctgggg cgtgatttac ttggtattgg
    14761 gaagtgttca cttccgctca tctaattatc tgcttctatc tcttttgtcg atagtcatca
    14821 gttttttttc tttgtttttt tccattatgt tgatcagttt ccaagtaaaa gaaagacaac
    14881 atcaggaatc gcttcaactg tatcaaataa atgaagaata ttaccatcat ctagaggaat
    14941 tcaaacatga ttataaaagt ctactgtttt cattaaaaat tactcttcag gaaaataaaa
    15001 aagaaacgtt ggaattttta gaaatattag aagatcattc tgcagctatt tttgaaaaac
    15061 cgcaaataca acagctaaca aatattgtat ccccagctgt caggggtgtc tttttaaaat
    15121 ttctagaaaa agcagaatcg gagaaaatcc cgatccaagt agttattcct gtcgaagtga
    15181 aaacaatccc gattgatctg gttgttttta ttcgctgttt atctattttt atttctaacg
    15241 cattcgatca tcgtgtagcc gatcagagtc cgattacgat caatctgctg gaagaaaaag
    15301 agggatcacg ttttagtata gcgaacaagg caaatccaac gaatttgacg ctatcggaga
    15361 tggtcaaaag aggaaaaacc tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa
    15421 aaatgttgga tgtttacgaa aatgcagaac taaaaatcga attttcaaaa cagaatcact
    15481 atttttttgt agaaatgtat ctggcatcta gaaagagcaa atcagcctac tgacttactg
    15541 tcaaaggata agtttgagga aagtctacac ggagatagag acaacaacac agcatattca
    15601 aaagaaacag aagcgtgatg accgttgtca cccttctgtt tcttttgcta tgtagttaaa
    15661 caaagtagtt gacacttccg ctacttagta ttagtacgaa agtatacttc atgatactaa
    15721 gtagataaga agtgaaaagc ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca
    15781 ccagataaag ggaagagcaa tatcgcttat tttaggcgtg gagaagcaag caaatcccta
    15841 cgatcacaaa aaaaatgctg acccagcagt taagagagtt agaacaagat gaaagtatcc
    15901 atcgaaaagc ctataaccaa gtcccgccta aagtagagta ctcgctcacc gaagaaggaa
    15961 aatcattaag agagattcta gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac
    16021 aaaaagcctc aagggaagaa gacacgctac tgcatacaca tggtggctac ctaaattatt
    16081 gattttctat gacatttatt ttaaataaaa gaaaacagga gtttttttaa aagcatcatc
    16141 tattttttat gtaaaaaaat ggtatgatta atcaagtaag aagtgattgg aagaaggcaa
    16201 gcaaatgaat agtatggaaa aagtcaacca gtttagagat gaacgtaatt ggcgacaatt
    16261 tcataacgaa aaagatttgg ctatctctat ttctttggaa gcatctgaat tgttagaact
    16321 ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga
    16381 actggcagat gtattgatct attcatacat gatggcagac aaccttcacc tggatctaga
    16441 tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc
    16501 aggtgtacga aaaaaataca cagagttgta gaaatgtgtc attcggctgt tggaaagagc
    16561 aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa
    16621 aaagatggct agtcaatatc ctcttgttcc tcttgttatt agtaggacta gctttgattt
    16681 tcaatgaaca gatcaaggat tattttgtca gagagacagg agataaatat gctatagcga
    16741 atgtaacaaa agaagatctg aaaaagaata acgataaaga tgtcagtttt gactttgatg
    16801 cggtagaacc aatgacgaca gaaggggtca tgaggtcgca aatgagagga acagatctac
    16861 cagtgattgc tagtatcgca gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct
    16921 tggataatac gtcactactt tacggagcag gaacattatc tccagatcaa gaaatgggaa
    16981 aaggaaatta tgccttagca agccatcgtg caacgaaccc tgaattgctg tttacaccgc
    17041 tagaaaattt agagatgggt gccaagatct atttgacaga tttagaaaat gtgtatacat
    17101 acaaaacatt ttttaaagaa aaagttgctc ctacagatac ccaattactg aatgaagtcg
    17161 agggaaaaga aattgtcaca ctaattactt gtggcgacat ggacgccgtc acgcgactag
    17221 ttgtacaagg agaactggaa agtgttacat cgataaaaga tgcgacagat gatatgagat
    17281 ctgctttcaa tctagagaca aaaacatttt aggatactgg gacataagtc gttacagatt
    17341 aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa
    17401 cgcaaagagg tgcatctctg atttggttgt tttctagata gacttgtgtt ccagtttttt
    17461 tattttttat atgaaaaggc ttgcataaat aaaaattcga tggtatgatg attttgaata
    17521 agaaatggat tgttactgta cggcaggcaa aaccagaatt cagctaagaa acagtaggcg
    17581 aattctaggt ttttttattt ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt
    17641 tgaataacaa tgtcgtcatt tcgaaaaatg gattcgggga agaagtcgtt tgtatgggaa
    17701 aaggactagc ttttcaaaaa aagattgggg acgagatttc gccagaagcg gtccaaaaag
    17761 aatttgttta agagattctt ttgctaaaaa ccaattccag cagctgatgg ctgatattcc
    17821 ggcagaggag atcgaatggg tcaaacaagt agtcgaactg gctgaagata aactaaaagt
    17881 tgaattttcg cctaatattt atctgacatt gaccgatcat cttcattatg cgatcacacg
    17941 agcaaaagaa aatatccaac tgcctaatcc attattattt gaaacaaaaa aattttatcc
    18001 gaaagaatat caagcagcga aagaggctct tttgttcgtg aaacaaaaga cgggtgtgga
    18061 actttctgag gatgaagccg gttttattgc ctttcatttt gtgaatagcc aacaaggaaa
    18121 cgaagacatg caagtgacga tgaccgctac cacaatggta agagatatac tgagtatcat
    18181 cagtaaattc ttcggcatcg tgttcgatga agaatctttg aattatcagc gtatcattac
    18241 tcatctgcag ttttttgccc agcgttatct caaaggggaa ggatctgacg agcaagatga
    18301 atttctctac gcattagttc aaggaaagta tccaaaagcg tttcattgtg tcgaacggat
    18361 caatgagtat cttcgacaaa cgcaaaaaca ggaaatgggg atcgcagagc agatttattt
    18421 aacgattcat attcaaagag tagtaagtga gaaaaaaagc atccaatcat aaggattgtt
    18481 actgtatggc aggcaaaacc tggattgaaa aaataggttc acgaatgttt gtgactgttt
    18541 ttttcaatct gggtttttat tttttaaata aaaaagaaag aaggaaaaaa caatggataa
    18601 tcaagcagtt ggtcgtcgtg tatgggaagc ggtaggagga caaaagaatg tcaaaagttt
    18661 agtacattgt gctacgcggt tgcgattcag attaaaagat gaatctttag cagatacgca
    18721 aaagttaaaa gaagacccgg acgtgatcca agtcgtacaa agtggcggtc agtatcaagt
    18781 agtgattggc agtaatgtag cagatgttta tcaatcgatc gtggatgagg aaggtcttgc
    18841 agtttcggag gatggaaaag aagagtccaa aaatattttg aatcgtctga tcgatattat
    18901 ctctagtata tttacacctt ttctaggggc gatggcagcc gccggggtat tgaaaggatt
    18961 cttatcattg gcaacggtga tgggctggct gacagctgat agcggtactt atcaaatatt
    19021 gtttgctgca gcagatggtg tctttacctt cttacctgtc atgcttgctt ttacagcagc
    19081 taaaaagttc aaagccaacc agtttttagc agtagcaatt gctatggcac ttgtgtatcc
    19141 agctatcaca gcagttgctg gtgcgggaga agctatcagt ttctttggga tcccagtgat
    19201 tctttcacca tctggttata cttcttctgt cataccaatc attctagccg tatgggttca
    19261 aagtaaatta gagccattcg taaaaaaagt gattccgcaa tttctgcaaa tgattttagt
    19321 tccgcttgtc gttttagtag tgatggttcc attaacattt cttgctttag gaccaatcgg
    19381 gacggttgcc ggtaatgctt taggcggtct cttcaactcg atttatggat ttagtccaat
    19441 cgtagcaggt ctaatcatgg gaagtttatg gcaagtattc gtgatgttcg gcatgcattg
    19501 gggattcgta ccgatcatgt ttttgaatat cgaacaatat gggtttgatg tattgatgcc
    19561 aatgcttctt ccagccatct tggcacaagg tggggctgct ttagctgtag cattgcgaac
    19621 aaaagatacg aaacttcgag cattagggat ttcttctaca gtgacttcgt tatttggaat
    19681 cactgaacca actgtgtatg gtgtgacatt acctttgaag aaaccattca ttgccgcttg
    19741 tatttctgga ggtattggtg gagctattat cggattttcc ggagtaaaag ctttctccag
    19801 cagtttggtc agcttattga cgattccgac tttcattaat acggtggatg gtgtagagtc
    19861 aaatgtgaca gtagcagtaa tagctacagg aattgctttt gttcttgcat tcgtaggtac
    19921 attgatttta ggatttgacg aacaaacaca agacaatcaa ttagaaaaca aacatgcgaa
    19981 tgcaggagaa ccgattactt cagcacggca taccttaaaa agtcctttaa cgggtaaagt
    20041 actgccattg agtgaagtac cagaccaagt tttttcttct ggcgtgatgg gaaaaggtat
    20101 cgctattgat ccagaagtag gagaactagt tgcacctgca gacggagaga tcacgactat
    20161 ttttccaact ggacatgctg tagggatcac aacgactgac ggagcggaga ttttgattca
    20221 tatcgggatg gatacagtag aattaaatgg gaatggcttt gagatcttag tcaaacaagg
    20281 ggatctcgtc aaagcaggag atttattgat tcgttttgac atcgaagcaa ttagagcagc
    20341 tggctatagc gtaatcactc cagttgtcat tacgaatact gatgcatttg ccgatatact
    20401 tgaattagac caaaaagaga ttattgcaaa cgaagatgtt ctggcaattg taaaataagg
    20461 aggaaatcag gatgcaatca cgtttttcag aaaaattttt atggggtgga gcaactgctg
    20521 ccaaatcaat tagaaggggc ttatgatcga gacggaaaag gactatccgt tgcagatgct
    20581 atgcctggag gtaagcagcg ctttgcgatt atcggcagtg aagaatttga ctggacgatt
    20641 gatcaagaaa aatatattta cccgaatcat cgaggaatcg atcattacga tcgtttcaaa
    20701 gaagatcttg ccttgtttgc ggaaatggga ttcaaatgtt atcgtttttc gattgcctgg
    20761 acacgaattt ttccaaatgg agacgaaggt actcctaacg aagcgggatt agaattttat
    20821 gatcagctga ttgatgagtg cttaaaatat gatattgaac ctgtgatcac gatttctcat
    20881 tacgaaatgc ctcttcattt ggcaaaagaa tacggtggct ggaaaaatcg aaaactgatc
    20941 gacttctatg aacgatttgc tcagacagta ttagagagat acagcagtaa agtcaaatat
    21001 tggatgactt tcaatgaaat caattcggcc tttcactttc cagctttaag tcaaggatta
    21061 gtaaaaagca atggagcagg agagtaccaa aatattttcc aagcatggca taatcaattc
    21121 gttgcaagta gcaaagccgt aaaaatcggc cacgaattgc gttcggatat ccaaatcggc
    21181 tgcatgatta tctatgcaac aacttacagc atcgatgcca atccagtcaa tcaggcagca
    21241 acaatgatcc aaaaccaaga attcaatttt ttctgcacgg atgtccaagt acgtggagaa
    21301 taccctgcat acacagcgcg tacccataaa aaatatggag tcgatccgga aaaattagag
    21361 caaacggaaa aagacttcca gttgctaaaa gaatatccag tggactacat cggatttagt
    21421 tattatatgt ctacggcaat caatgagact gatccacaag cagcaacttc agaagggaac
    21481 ttgctgggag gagtaaaaaa tccgtttctt gaggcaagtg aatggggctg gcagatcgat
    21541 ccagaagggc tgagaatcgc attgaatgaa ttatacaatc gttaccagaa accattattc
    21601 attgttgaaa acggtcttgg agccattgat catatggaag aggatggcac gattattgat
    21661 gattatcgga tcgattattt acgtcgtcat atcgaagcga tggctaatgc agtagcagac
    21721 ggtgtagatt tgatgggcta tacgccttgg ggatgtatcg atcttgtcag tgcatcaaca
    21781 ggagaaatga gtaaacgtta cggctttatc tatgtggatc tagacgatga aggaaatggg
    21841 acactgaatc gatcaaagaa aaaatctttt gattggtata aagaagtaat tgcctcaaat
    21901 ggcgtaaact tgtaattaaa gcagctgaat gaaaaagaac acacttctca catgtgattt
    21961 gtgttctttt tttatttttt atgtgctatt cttttagtaa tagtaaagca aagggtgacg
    22021 ttatgaaaag gacacgtgta ctatacttgg aagtcgcaga taaaattaaa gaagatattt
    22081 tctcaggaaa atatccagtc ggttcgatgt tgccgacaga aagtgaatta gaagaactgt
    22141 ttcaagtcag caaaatcact gtccgaaagg caatcgaact gctggcatca gatgaatatg
    22201 tcgcaaagaa aagtggtcgt gggacgacgg ttctaagcaa tcgtccttac aatcgattat
    22261 ccaaagcggc aagctttacg caaatcttgg agaactcccg atatgaagtc cggaaagaaa
    22321 cgctggattt gcaattggtc catataaaga aggaagatcc tttgttttcc tttttcggtg
    22381 agcaagctgt ttgttttaag cgcttatatt atttgaacaa tcagccttat atttattttg
    22441 ttcattatct tccacaagaa atgatgcaat tcagcagaga agaatttggc aaagaatctt
    22501 tgtatcgttt gctgacgaaa aaaggatata ctattgactc attttctgat gatttctcgg
    22561 cagtattttt aacagaagaa gaacaaggaa ttctgaaaac agacgcacag atcggcatca
    22621 aacggatacg gaaatcgtct acgcaaattg gggaaatgat tgaatattcg gaggcggtct
    22681 accatacgga gctgcatcct taccatattg attatgaaac ataaataaaa aaagaggttg
    22741 tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt
    22801 tttcgtttgg ttaggcttat taccgcagat acacggctgt ttctggatta ccatttattc
    22861 tggataaggg ggtgtggcac gattttcaga aggaatctat gaaccaatca ctgtattgat
    22921 tttagcttcg atggtaaccc cattcggctt gacgatcgct tatttcttag ggaaaatcat
    22981 tcgtaaaaat atactcaatc gtcaagaaat cgatacattg aagaccgctt ttcctatggg
    23041 gatctgtcag atcacagagg gatgtttccc tatcgtattg aacgacttgg cacgcaatgt
    23101 catagcaacg ggagtcggtg ggacagtagg tggcggatta agcatgtttt ggggcgcaga
    23161 tagccgtatt ccggcatcgg gaatgtttgc agtcgctacg atgaccagac catgggcatt
    23221 cattggtgca ttgttagctg gttcttttgt cacaggaata accattttat tattgaaaaa
    23281 accagtagat ccgaatgcag aaatcatcca agaagaaaaa gaggaagaag acatatcttg
    23341 ggatgatttg acaattagtt aggagaggca gaatggatta caagaaaaaa gtacaataga
    23401 tgtaccttga gacaggaatg gtatgaaaga atcagagtta gaaagaatcg aatatacaga
    23461 ttttggttta ggagcgatcg aaactgtcca gagcatcggc accctccttg aaatgacgat
    23521 gaaggaaaaa tgagaacctt tcgttgtcga aagggtctgg tataccttat gtcgaaggaa
    23581 aggaaacaaa aaaactttcc tgtactattc caaaaaggaa agaaagttat tatacagtaa
    23641 aaaaagaaat tattcttcat gctagagatc agtacacgtt cgagcccaat atcaaacact
    23701 agttccaggc aggcgcaaca ggtgcggtta tcactgaatt ttcttccagc agtgacgatt
    23761 caagtgatat ttttacaaat ccagctattt atcgttaaca aaaagatttt taggagaagc
    23821 tgatgaacaa aaatgatcaa attttgatcg tttagttcgt cggctttttt atttagagaa
    23881 acatcattta tcagtgaaac ataacacgag aacaaaatga ttttttcgtt aataaaagaa
    23941 acaagtattt gttgtttaaa ataatacacg ttattggcat ataggacgaa atcccagtat
    24001 cttctataag taagtcattt catatcgatg aaaacacgtt atttattttt gttttatcta
    24061 taaaatggct
//
LOCUS       DASJSZ010000041        23713 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.041, whole genome shotgun sequence.
ACCESSION   DASJSZ010000041 DASJSZ010000000
VERSION     DASJSZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.041"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(170..535)
                     /locus_tag="U4B03_001494"
     CDS             complement(170..535)
                     /locus_tag="U4B03_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068351.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            743..1225
                     /locus_tag="U4B03_001495"
     CDS             743..1225
                     /locus_tag="U4B03_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068352.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1422..2312
                     /locus_tag="U4B03_001496"
     CDS             1422..2312
                     /locus_tag="U4B03_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEN2068353.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2638..5112
                     /locus_tag="U4B03_001497"
     CDS             2638..5112
                     /locus_tag="U4B03_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2068354.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5215..6951)
                     /locus_tag="U4B03_001498"
     CDS             complement(5215..6951)
                     /locus_tag="U4B03_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN2068355.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6948..7652)
                     /locus_tag="U4B03_001499"
     CDS             complement(6948..7652)
                     /locus_tag="U4B03_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2068356.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7783..8286
                     /locus_tag="U4B03_001500"
     CDS             7783..8286
                     /locus_tag="U4B03_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEN2068357.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8246..8584
                     /locus_tag="U4B03_001501"
     CDS             8246..8584
                     /locus_tag="U4B03_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEN2068358.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8605..10023
                     /gene="ffh"
                     /locus_tag="U4B03_001502"
     CDS             8605..10023
                     /gene="ffh"
                     /locus_tag="U4B03_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEN2068359.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10135..10713
                     /locus_tag="U4B03_001503"
     CDS             10135..10713
                     /locus_tag="U4B03_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEN2068360.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10717..11238
                     /locus_tag="U4B03_001504"
     CDS             10717..11238
                     /locus_tag="U4B03_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2068361.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11317..11871)
                     /locus_tag="U4B03_001505"
     CDS             complement(11317..11871)
                     /locus_tag="U4B03_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN2068362.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12124..12399
                     /gene="rpsP"
                     /locus_tag="U4B03_001506"
     CDS             12124..12399
                     /gene="rpsP"
                     /locus_tag="U4B03_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEN2068363.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12411..12662
                     /locus_tag="U4B03_001507"
     CDS             12411..12662
                     /locus_tag="U4B03_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEN2068364.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12786..13577
                     /locus_tag="U4B03_001508"
     CDS             12786..13577
                     /locus_tag="U4B03_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068365.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13747..14268
                     /gene="rimM"
                     /locus_tag="U4B03_001509"
     CDS             13747..14268
                     /gene="rimM"
                     /locus_tag="U4B03_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEN2068366.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14258..15019
                     /gene="trmD"
                     /locus_tag="U4B03_001510"
     CDS             14258..15019
                     /gene="trmD"
                     /locus_tag="U4B03_001510"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEN2068367.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15155..15502
                     /gene="rplS"
                     /locus_tag="U4B03_001511"
     CDS             15155..15502
                     /gene="rplS"
                     /locus_tag="U4B03_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEN2068368.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15812..16054
                     /locus_tag="U4B03_001512"
     CDS             15812..16054
                     /locus_tag="U4B03_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEN2068369.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            16405..16659
                     /locus_tag="U4B03_001513"
     CDS             16405..16659
                     /locus_tag="U4B03_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HEN2068370.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
     gene            16688..17947
                     /locus_tag="U4B03_001514"
     CDS             16688..17947
                     /locus_tag="U4B03_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN2068371.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18000..18896
                     /locus_tag="U4B03_001515"
     CDS             18000..18896
                     /locus_tag="U4B03_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2068372.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            18907..19776
                     /locus_tag="U4B03_001516"
     CDS             18907..19776
                     /locus_tag="U4B03_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2068373.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            19786..20461
                     /locus_tag="U4B03_001517"
                     /pseudo
     CDS             19786..20461
                     /locus_tag="U4B03_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            20667..22331
                     /gene="alsS"
                     /locus_tag="U4B03_001518"
     CDS             20667..22331
                     /gene="alsS"
                     /locus_tag="U4B03_001518"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEN2068374.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22344..23054
                     /gene="budA"
                     /locus_tag="U4B03_001519"
     CDS             22344..23054
                     /gene="budA"
                     /locus_tag="U4B03_001519"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEN2068375.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23184..23681
                     /locus_tag="U4B03_001520"
     CDS             23184..23681
                     /locus_tag="U4B03_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEN2068376.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         7847 a         4075 c         4933 g         6858 t
ORIGIN      
        1 tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt ttggagagaa
       61 gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatataaga aaaacatata
      121 tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt tatggatgcc
      181 gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt ggaactaagt
      241 aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag aagtaacgcc
      301 attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata ttggcaatga
      361 ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga gataaaaata
      421 tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag gcgaaatgca
      481 cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt atcatctttc
      541 atcaccacct aacaagtgtt attattttgt tagttttatt gtaaattgaa tcctgcaata
      601 atcaattact ttttcattat ttctagatta aatgaataaa ctctcatgcc attatctcta
      661 aaaaaaaagt ttttccccct aattttttta gatagaaaat tgtatagtat gatgagagaa
      721 aaagtagttt gggggaacgt taatgagaag aaaagcagta atcgatcggc tttatttatt
      781 tttttggttt tttcctatcg ttttgctacc ttttacatgg gtaaatgctg ttgtacaagg
      841 tgcggagata ttaggcctga tccaaggaag cggattagtg gtttctagga attttccagc
      901 tttgactgtt ttctatttag tgataagtgt aggtctctta ttaccaaaat ctctgatcaa
      961 tcactacaat tggcagctgc tttacttttc ttgcttatta gtatttacat ctatgttttc
     1021 tttgctgcct cgcttgatta tcggccctaa tggtttgagc gagagtggat tgaaggattc
     1081 agcttattcc tccatgtatt tcttgacgat caaaccatgg ttctatttag taattgcctc
     1141 atttattctt gcaacatttt tttacttctg gacagaaatc aggataaaaa gtgaacgatc
     1201 tgtatctata aaaaataact attgatggtg gatcatcagt aaaaaagcat atgagctgcg
     1261 taaaatgcat gtttctcata tgcttttttt attattatgt aaagaatgcg aaaaacgttt
     1321 acatgatttt tacataactt aactatattt ttacagcaac catacgaggg atttacagtc
     1381 gactgataaa ctttagttgt aaataagaaa ggagcaataa aatgaaaaaa gtattgttta
     1441 ctctaggtat gatgggaatt cttttagccg gatgcggctc aggatcagga aatacagatg
     1501 gctctgccac acaaaataat tcaagtgact caaatgaaca agtaaaaatc gttgcggtag
     1561 gttcgacagc attacagccg ttagtcgatg cagcacaaga aagctttgta caagaaaatc
     1621 caaattatca aatctctgta caaggaggag gaagcggaac tggtttgagc caagtcgaag
     1681 ctggagcagt tactatcgga aactctgatg tatttgcaga agaaagagac ggagttgatg
     1741 cttcaaaatt agtggatcat aaagtagcag tggtcgggat ggcaccaatc gtcaataaag
     1801 atacagatgt caaagatatc accaaacagg aattgattga tatctttaca ggaaaaatca
     1861 ctaactggaa ggaagttggc gggaaagatc aaaaaatcaa cgtcgtgaac cgtgcaaacg
     1921 gcagcggtac ccgcgcgaca ttcgaaaaat ggggactgga tggtgctacc cctgtgcagt
     1981 cccaagaaca agattcatca ggaacagttc ggcagctggt aagtcaaaca ccaggagcaa
     2041 tcagctattt agctttctca tatctcgatg attccacaca agcactaagt atcgacggtg
     2101 tagaaccaaa agaagagaat gtagcagata acagctgggg aatctggtca tatgaacata
     2161 tgtacacgaa cggaaaacct tcccctgaag tccaaaaatt cttagactac atgatgacag
     2221 aagaaatcca agaaggacct gtcaaagaat tgggctattt accaatcaca atgatggaag
     2281 tagaacgtga tcacgaagga aatatcaaat aactagcaga taaacatgcc acggtagtcg
     2341 ttactattaa ggaaaaatca actatgtttc cccacatata catttttcct ttaattgtga
     2401 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
     2461 accgtttatt cgaaaaaaag agctacgaca tagaactgtc gtagcttttt ttttgctata
     2521 tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa cactttccat atctgttcta
     2581 tactggatag gagaagtttg gaaatatcca gaaaacgaaa tcaatcgagg tagccacatg
     2641 aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa cacaaagatc atcagataaa
     2701 gtcatccgtc tttttccatc gtataaagaa ggattaacgg cagatcaagt caaggaacga
     2761 atagaaaaag gtgcagcaaa taattcagtc gatcccacat tcaaaacaaa tcagcaaatc
     2821 gtattggaaa acatatttac gtatttcaat ttgatctttc ttatcttagc cattttgctt
     2881 tgtttagtag aatcctataa gaacttgact tttttaccag ttatcatagc caatacagga
     2941 atcgccattt ttcaggaaat tcgatccaaa aagatcttgg acgaactaaa tgttttaaat
     3001 acagcaaaag tcaatgtcat acgagacggg atagaacaaa caatagatat agaagacctt
     3061 gtaatagatg acattgttat tttagagact ggacatcaaa ttccagcaga tgcagaggtc
     3121 gtggaaggga aactgcaagt taatgaggct ttattgacag gcgaagcaga tgaaataacc
     3181 aaagaaatag gcgatccatt aatgtccggc agtttcattg tttccggaaa agcttacgcc
     3241 cgattagaca aagttggagc agattcgtat atttcccagc tgacagtcaa agccaagaca
     3301 atgggagaag gagaacagtc ggagatgatt gcttctctta atcaattgat caaatggatt
     3361 gggatcatca ttattcctat cggtatcctg cttttttctc aaagttactt ttttaatgga
     3421 aacacgatca aagaaagtgt cgttgcaatg gaagcagcat tgatcggtat gatacctgaa
     3481 ggactgtact tgctgacgac gattgcatta gcattaagtg cgactaaact agcaaagcaa
     3541 agagttcttt tacacaatat gaaaagcatt gagacattgg caagggtaaa tgtcctttgt
     3601 gtagataaaa caggaacgat cacagaaaat aaaatgtctg ttcaaaaagt gatcgtctct
     3661 aaaaaacaag aaataatcag tcagtcagat caattggaag catgcattgc tgactatgca
     3721 aaagcgatgg caggagacaa tgcaacaatg gaagctgtta aagcatattt tacaaaaacc
     3781 actgatcaat cctatcacca tgttattcct ttttcttctg tccaaaaatt cagcagtgta
     3841 gcgttggcag acaaggtgta tgttttagga gcgcccgaaa tggttttgcg cgatcaattg
     3901 ccggaatatg cggaagagtt tgtttctttc gctgaaaaag gctatcgcgt gttggtcttt
     3961 ggtgtgtacg atgggattct tgaagaacca gtattgacag aagcagtcac accattaggc
     4021 tatattctga ttgctaatcc tatcagaaaa gaagcaagag ccacatttga ttacttcaaa
     4081 aaacagcaag tagccatcaa agtgatttct ggagataatc cgctcaccgt atccaatgta
     4141 gctgtccaag cagggatttc acatgctgat cagtacgttg atgcatctaa attatcagaa
     4201 gaagaatatg aagcagctgt cgaaaaatat acagtttttg gacgcgtcaa gccagaacaa
     4261 aagaaaatct tcgttcaatt gctaaagcgt cgtaacaata cagtagctat gacaggtgat
     4321 ggcgtgaatg atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga
     4381 aatgaagccg ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg
     4441 ccagaagttg ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg
     4501 tttttagtga agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg
     4561 acctatcctt tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca
     4621 tctttcttgc ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat
     4681 gtgatggaaa aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg
     4741 atctgcgggg tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt
     4801 ttgattgcag tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga
     4861 agtcagatca ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag
     4921 ctgttttcaa tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc
     4981 tcggcagatt cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa
     5041 aatgaaaaag agcaaaagcc gtttagtcgc gtagttaaaa aatttctatc gatatttaaa
     5101 cgagaaaatt gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc
     5161 tttcgaagca ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat
     5221 tttcggaatg gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt
     5281 accatgaagg agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga
     5341 atgccggctt cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc
     5401 aatgtcgata cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata
     5461 gcggataata atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt
     5521 gataattgga taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg
     5581 aatcgtcaac cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata
     5641 aggttctgta taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat
     5701 gatttcccga atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga
     5761 agtcagagtt tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag
     5821 tgaagtcaca ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt
     5881 ttctaaacgt cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc
     5941 tgaattagta aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat
     6001 ctcctgatgg atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg
     6061 ctcttgaatg acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag
     6121 ctgcatcttt ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc
     6181 aatcatcagt tcgttcagta acgtatataa ttgctcttct gtggtagtat acgcccgata
     6241 catcttactc atttgttcgc tcaacgcatt gatcgtctga tacaattctt cccattgatc
     6301 gggtgtttgc atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg
     6361 cagcacggtt ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag
     6421 aacaaggaag aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg
     6481 taaaaaaccg cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt
     6541 gactggtaat gcgacatata aaagatcccc attcaaagta gtacttttgc gcaaagcgct
     6601 acctaggttc ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt
     6661 caatgtttca ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg
     6721 ttcgttcgac tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt
     6781 ttcgacatct aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg
     6841 ttccacgatc tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact
     6901 aactaaaaga ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt
     6961 tcctgaaact tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct
     7021 cgctcgattt tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg
     7081 aagtcaaaat tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt
     7141 ttcatgaaat aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct
     7201 acggtcactt gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc
     7261 tcagcttcat cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg
     7321 gctaatactt cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg
     7381 ccaatgatcc gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt
     7441 ttttcttgac gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct
     7501 aaaatgatga aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg
     7561 gcactggtta ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa
     7621 ggttcatcat caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc
     7681 tgtttatatt ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta
     7741 taataggaag gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca
     7801 tgtttaggcg gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg
     7861 gaactagttg aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca
     7921 attcctagag atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa
     7981 gcaaaagtat ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc
     8041 aaagcctatc aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc
     8101 ccttcatgcg gaacccattc gatttatgac gggagttttt ccggaataaa aaaaaatggt
     8161 gccggagtgg ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa
     8221 tggctgaaaa ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct
     8281 ctttgaattt tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta
     8341 tgcagatgat ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt
     8401 atatgacaat atcaagcgga caagcaagat cttggaggat tatgaaaaaa aacttcatct
     8461 tttttctaat tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga
     8521 taagtacccg gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga
     8581 ataaataaaa ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag
     8641 gcgatgggaa aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg
     8701 cgggaaatcc gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt
     8761 actaagcgcg tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca
     8821 caacaaatcg taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca
     8881 gagttaaata aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca
     8941 ggtaaaacga cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt
     9001 ccgctcctaa tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta
     9061 ggtcaacagc tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc
     9121 gtccgtcaag ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg
     9181 gcaggacgtc tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg
     9241 acgcaaccaa atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac
     9301 gtagctgata gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat
     9361 ggggatactc gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa
     9421 ttcatcggtt ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct
     9481 agccgtatcc ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactat
     9541 gacgagaaaa aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat
     9601 gactttattg aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa
     9661 atgatcccag gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat
     9721 gttgaacgca aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat
     9781 ctgttgaacc caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa
     9841 gtgaatcgaa tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa
     9901 ggggatatga acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta
     9961 ggcaaaatgg cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa
    10021 taagatgaaa aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat
    10081 cagcaatttt tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga
    10141 tgtccgtgga gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt
    10201 cctgaatatg atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga
    10261 ttgagctggc agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc
    10321 gatattaaaa aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa
    10381 ggaattattc gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg
    10441 aaaattcaag aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa
    10501 atcgttgatt cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat
    10561 gaattagcaa aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat
    10621 gcttttttag aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa
    10681 gaggtcgtac gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt
    10741 gctataagtt tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac
    10801 actccaacag gtaagcaaac cacttatgaa gctttttatg aaacaatcga tacgaccatc
    10861 atgggcagaa aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca
    10921 gagaaaaaga atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc
    10981 gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc
    11041 tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa
    11101 tggtatatcc agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga
    11161 acatatgaag cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg
    11221 cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc
    11281 tgtacatagt tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa
    11341 atctagatac gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc
    11401 attttcatca attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg
    11461 cacattcaaa aagacaagtg attgtcgtag atggtggttt gcgccaaagc cgccgatccc
    11521 acctggtgat gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg
    11581 tcttgagcct acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg
    11641 agtaacgaaa acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc
    11701 aggagcctgt tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac
    11761 aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt
    11821 gtaagaatct tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt
    11881 cctccttcaa actatctata acaatcttac aaaaaataag taactggagc aaataaaaac
    11941 acttactgac aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta
    12001 aaatcttcta tttcctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta
    12061 cagggtaaaa aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa
    12121 ataatggcag ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt
    12181 atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact
    12241 tacaatcctt tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg
    12301 ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt
    12361 atgcaaaaac accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt
    12421 taagcgactt agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt
    12481 tagacgtcgt agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata
    12541 ttggccgtat cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg
    12601 gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt
    12661 aaataaacgt gctgtactcg aattttcgag tgcagctttt ttgttttttt attaattgaa
    12721 gaaatgatta tttggcaaaa caagcaagaa agcgtatcct tataggagga gggagtgaac
    12781 agccaatgag aaagaaaatg ttatttattc catttgctct attgttaagc gcttgtagtt
    12841 cggttactca agaatcagac aatacacatc tatcaagcag tatatctgaa actgttacca
    12901 gtccagaaaa aagtagtacc aaaacggcta ccaccactga acaaacaatg gctacctcaa
    12961 ataatgagaa aaagactgct ttagaccagt tgaaagaaca acagccaaat gttcccatgc
    13021 ctctagatgt accagtctct tcaggctatc tcaatatcgc agcgacacat acaaagcaag
    13081 gttattctat tttatattac agaacagatc gtcccctcgg gttgaatgct gacgaattga
    13141 atcaagaaac gcctattgca acctatctat atcaatatgg gtttgcttct agtcaagaaa
    13201 caatccaagt tttgcagccc tttgagatcg acacaaatgg tcagcaagtg gacttaggtt
    13261 ctcgaatcac agggtatcag caaggtgcag caggttcaag ttttcttgaa tggcaagaag
    13321 gaaactggcg tattcgaatc cgtgggaata atatagaagg ccaagatccc ttactattag
    13381 ctaaagaaat cgtagcttat ttggaagaaa acagtctacc agcacctgaa caatttggta
    13441 aaattactgt tgatatgggt gatacgacga atcgagccgt agaagtaagc tggcaagaac
    13501 cgaaaaatgc ctatacgatc actcatcaag atcctatgtc tgcgttgaag atggcagttt
    13561 ctatgaagag gttgtgaaat cagcgcgctg atctgagccc atcatttaac tccaagtcac
    13621 aggaagtaat caaaaatagc acgccgcatt tttgcataat tttggcttaa taccttaaaa
    13681 gaaaactaga attgcctctt cacacatgct aaaataaaac agacaaaaaa gaaatgggga
    13741 tataagttga ctgaatactt gaatgtagga aaaatcgtaa acacacaagg tatcaaaggc
    13801 gaagtccgtg ttatctcaac aacggatttc ccagaagaac gttacaaaaa aggaacggtg
    13861 ttaacactat tccaagacgg aaaagcacct gtggaattaa cagtcaaaag tcatcgcaaa
    13921 cataaaaatt ttgacttatt gagttttgaa ggacatcctt ctatcaatga tgtggaaaaa
    13981 tatcgggatg gcatattaaa agtgtcaaaa gacaactctg tagatctagc tgaaaatgaa
    14041 ttctattacc atgagatcat cggtctgcat gtattagaag atggaactga gttaggtaaa
    14101 gtaaaagaaa tcttgtcacc aggtgccaat gatgtttggg tcgttcaacg tccgaaaaaa
    14161 gctgatatat tgcttccata tatcgcttct gtcgtcaaag aagtcgattt agaagctgga
    14221 gtggtccgtg tagaaattcc agaaggactg attgacgatg aagattgatg tattgacttt
    14281 atttccaaga atgtttgaag gtcctatggg ggaatccatc atcggtaagg ctgtagataa
    14341 aggattatta gatatcaata tctctaactt ccgcgattat tcagataaca aacatcagac
    14401 agtagatgat tatccatacg gcggtggagc aggtatgctg ctcaaagtcc agcccatcta
    14461 tgataacatc caagccattg aaaaagctgc accggatgtc aaaaaaagag taattcttct
    14521 tgacccagct ggtaaacgat tcgatcagaa aatggcagaa gaattttcac aagaagaaca
    14581 cttagtattc atttgtggtc attatgaggg atatgatgaa cgtatccgca cattagtgac
    14641 agatgaagtt tccttaggag attatgtcct tactggggga gaattaggtg cgatggtcat
    14701 gatcgatgca acagttcggc ttttgccaga tgtgttaggg aatcagactt cagctcaaac
    14761 ggattcttat tcaaccggtt tattggaaca tccccaatat actcgcccgg cagaatataa
    14821 agggatgaaa gtaccagaag tattgacaaa tggaaatcat aaattgattg aagaatggca
    14881 attaaaagaa tcactaagaa ggacctatca acgtcggcct gatcttttgg aaacacttga
    14941 atggacacct caaatgacga aatttttaga agaaatcaag aaagaagaac aagaaaaaac
    15001 agcagatgaa gccaagtgac tttttctaaa atattcttta cagtaggttg ataatatgtt
    15061 agtattttat ggtgagtgca aaagcactta actattacga tattccgcta tgagaaaagc
    15121 tcataagaat atttggaata aggagaagaa aaccatgaat ccattaatcg aagaattaac
    15181 aaaagaacaa ttacgttctg acatcccagc gttccgccct ggtgacactg tgcgtgttca
    15241 cgcaaaagtt gttgaaggta ctcgtgaacg tatccagtta tttgaaggtg ttgttatcaa
    15301 acgccgcggt gctggaatca gcgaaactta tacagtacgt aaagtttcta acggagtggg
    15361 cgttgaacgt acattcccat tgcatacacc acgtgttgca aaaatcgaag tagttcgcta
    15421 cggtaaagta cgtcgtgcaa aattgtacta cctacgtgca ttacacggaa aagctgctcg
    15481 tatcaaagaa atccgtcgtt aatcattgtt atcagactga gaaagaaccc ttggtattca
    15541 agggttcttt tttcttagca tgtaatttga acaccctgat ccagtttttt tctttaatta
    15601 ttggtagggg agaataatgg attagaataa atctacctat aagtagtagt aaaaaaaaga
    15661 gcactatgat aatatctttc ttaagagtaa ccgctttaat aaaaaatcca gaatattcta
    15721 acataaatgg agtattctta gaatcaaaag gagaaagaag atggtttata cttatacgga
    15781 gcaagaaaaa atgaaagttt gagacacatt catgaatgat ggaaaagtaa aacaagtacc
    15841 aagttcaaca aaaaagaaaa acatcctatt gaaagaagtc ttgaaacgct ttgaccatgg
    15901 agtgacttat acagaaacag aggttaattc gatactgctg aacgtatttt ctagtggcga
    15961 ttatgtcgaa caaagacgtt atctaatcac atttggcttt ttcaaaagaa gttcagacgg
    16021 tcgggcatat cagatgatgg gaattgaaaa ttagaagttt taatttatac tcattgaaaa
    16081 aagttcaagt atgcgatatt ggttctacac cgcatacttg aacttttttt gtcatctgta
    16141 tctataatac aaagccaaga acgttattca aaatatggat agctatgata aaagctaatg
    16201 agcgattcgc agttaaataa gcaatcgtca gtaaaatcgt tccaggagcg tacatgaaaa
    16261 aatcaattgg atgcgttaca tgaataaaga taattctctt ttatttgtac gaataaatac
    16321 caaaacgtac ctcctaaaat tagattcaaa gaagatagca aagaggaaaa agatagttct
    16381 gcagcagcta tagcggcatg tggattgcta gaactttcca aacaactgcc tgtttcagat
    16441 cctcgtcatt tcgattacga agagatagca atcaaaatct tggaagaatt acagaaaaac
    16501 tatacaacga aacgagttaa cggatcaaac ggtctgcttc tccatgccgt ttatgataaa
    16561 aacagcttaa aaggtgtaga tgaatgtgtg atctggggcg attactttta tgtagaagga
    16621 atcactcgtc tagctaaaac atggtactgt tactggtgat gtataagaga agaggaggat
    16681 tcatataatg aagcgatgga aaaaaatgat gattggatca atagttttag gaatgtcact
    16741 cgtatttggt gcttgtggta atcaagaaga cagtagtgaa gaatcagcaa atgaaattac
    16801 cgtttcgaca tggaattatg aaacaacacc tgaattcaaa gcgctctttg aagcattcga
    16861 aaagaaaacg ggaatcaaag tgaaagcagt agatattgct tcagatgatt atgatacgaa
    16921 actcaccacg atgctttctt ctggagacac gacagatgta ttgacgatga aaaatctttt
    16981 gtcgtattca aattatgctt tacgagatca gttagtcgat caaacagaac gaattagaga
    17041 attagacact gatgctgctg aagaaacata tgaaatgtat gatatcgatg gaaaaacata
    17101 tgcattgcca tatcgaacgg acttttgggt actatattac aataagaaaa tgtttgatga
    17161 tgcaggaatc gaatatccaa aaaatctaac ttgggacgaa tatgaagatt tagcaaaaaa
    17221 acttagtaaa aatgacggac aagtttacgg agcatatcaa catatctggc gttcgacaat
    17281 ccaagctatt gcagctgctc aaaacgaggc gaatcttgtt gaaccagatt atcaatatat
    17341 gactgattat tatgatcgag cattgaggat gcaaaaagaa ggcgcgcaga tggattttgg
    17401 cacggctaaa tcaacgaaag taacctatca gtctcaattt gaagaacaaa aagcagcaat
    17461 gatgtacatg ggaacttggt atatggctgg catactcgca aacaaagaag caagtaagac
    17521 agaagtagaa tggggaatta cagctattcc tcagaagaaa aaaggagaat cggtcacgac
    17581 gtttggctca ccaacagcat ttgctgtcaa taaaaactca aagaaacaaa aagcggcgca
    17641 agaatttatt gaatttgcag ccagtgaaga aggagcaaaa gtattagcag gagtaggagt
    17701 agttccttca tatcgtacgg atgagattga tcagctttat tttaatctcg ctggaatgcc
    17761 aacggatgaa atttctaaaa aagctttttc tcctgatgaa atcaaacttg aatttccgat
    17821 tgatacgtat gggcctgcaa tcgataagat tttacaagaa gaatatgact tagttttggt
    17881 tggagatgaa acgccggaaa aaggaacggt aaatatggag aaacgtgtag ctgcggaaaa
    17941 agaataatca tgaaaggaga gaagccaaga gataaaacac tcttggtttc gtcatcgaaa
    18001 tggagaatag tataaataag aaagctacaa atcatttgcg tagaaaacga acgtggatcg
    18061 cttattcgtt tatcgccccg aatttcattg gttttttcgt ttttacatta ataccaatcc
    18121 tgttttcgct tgtactagca tttatgaaat gggattcttt ttcaacgcca gaatttgctg
    18181 gactagataa ctttcgtaaa atggccaacg atgagacatt ttggatttct ttgaaaaata
    18241 cttttttata taccgctgct gtagtgcctg gcacacttgt ttgttcgtta gggctagctt
    18301 tactactaaa tcagaaaatt agagggatga agctttttcg tacgatattt ttctttccgt
    18361 atgttacttc cttagtagca attgctgttg tttggaatat gttgtttcat ccaacagtgg
    18421 gacctattaa tatcttttta agacgattta ttgaacatcc tccaggttgg acttcaagtt
    18481 caaattgggc gctttgggca attgtaattg tcagtatttg gcgagggatg ggttattaca
    18541 tgattctata ccttgcagga ctacaaagta tccaaaaaga actatatgaa gcgtcttcgc
    18601 tagatggagc aaaccgttgg aaacaatttt tgaatgtgac attaccttcg ttgagaccaa
    18661 caaccttctt tgtaacgatc atgcttgtca tcaattgttt caaggtgttt gaccttgtgc
    18721 aagtgatgac ggatggcggc cctggtcgag cgacaaatgt gctggtttac caaatctaca
    18781 atgaagcatt caaaaaattt gattttggtt atgcttcagc tatttcactt gttttgttcg
    18841 taattgtttt gggcgttacg gttgttcaat tttcttggaa taagcgtcgt acttaggagg
    18901 tgtgatatgg aagagagtgt tcgaaatgaa agaaaaaaag caaaaattcg ttcaaaaaac
    18961 atgcccttta ttcaatggtt gggtatgatt ctgctgattg tattagcttt attgacgatc
    19021 attccttttt tatggatggt ttctgcttca ttcaaagcaa ataatgaagt gttaacgatt
    19081 ccgattcaat ggatccctaa aacttggcat ttagagaatt atctggttat ttggtctcgt
    19141 attcctttag tgacattttt taagaataca gcttttttaa gcgttgtgat cacgttgatt
    19201 cagttgttta cttctagttt tgctgtttac gggttttctg aactaaattt taaagggcgc
    19261 gatattttat ttttagcata tatcggcacg attgctgtcc cttggcagtc ttatatgatc
    19321 ccgcagttca ttatgatgag aaaagtgggt ttaacagata cgttgtggtc gttggtgcta
    19381 ttacaagcgt tcaatgcttt tggtgtgttc ttgttgaagc aatattacag tagtatccca
    19441 gattctttat gtgagagtgc gcggatcgat ggcttatcag agtgggaaat ctatcgcaag
    19501 atcattttac ctcttacaaa accggcgatt gccagtttga cgatcatcac attcgttaat
    19561 acatggaacg attatatggg gccattcatt tatttgtctt ctacggaaaa taaaacaatc
    19621 cagttagggt tgaaaatgtt tattgggcta tttgatgcag agtacgcatt aatcatggct
    19681 gcctcagttg tttctgttct tcctgttgct tttatatttt tagcgatgca aaaatatttt
    19741 gtagaaggga tcgcttcatc aggaatgaag gaataggagg tgattatgaa agagcagcgt
    19801 tttgacaaaa atgtttatat ggaattcttt tattggttgt atcaatcatt ggcaagtagc
    19861 ctttgttttt ggtgtgtcaa tatcttgtat atagcagctg cgttcctttt gtcgatagat
    19921 ggaaggaatg tcttttcctt tattttttct ttacttttta ttggtccagg ttgcatcgtg
    19981 atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt acgtttgttc
    20041 ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt tgcttggata
    20101 gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta tggaaaatgg
    20161 ttgattccgt tgtttgtttt acttgcttgt tttacttgct tgtttatcag tttcctttag
    20221 catcaactgc tggtattttc aggtgagaaa tccagcgtcg aaaccaaatc aagttctacg
    20281 gatagcgttc tactatacgt tgaaaaaatg gtatgtgagt ttactagatt ttttactatt
    20341 gacaagtttg tttctcttct ttttcgtaaa gccccaatgg tgtattctac taggtccttc
    20401 tatcgtattt ggtttgattt acttcaacaa tcgtaaattg atgagaacta tggatctgta
    20461 gactttataa attgaaactt tcaattaaga atgacagttt tttatgtaaa aactgtcatt
    20521 ctttttcatt ttttgaaaca tatggctaaa caaatgaaat aaaacatgct atacttttga
    20581 atgaaacagg tttagaaact gtatcactta tttgtgatta tttctaacta ataattaaaa
    20641 ataaagcata aaaaggacga tgttcaatgg ctgaaaaaat gaaacaaggt tcgaaatctg
    20701 tagtagaaag tttgattaat catcatgttg attatgtatt cggtatacca ggtgcaaaaa
    20761 tcgatggtgt atttaatgaa ttagaggatc aaggtcctga attaatcgtg actcgtcacg
    20821 aacaaaatgc tgcatttatg gcacaagcaa tcggacgtat tacaggagag ccaggagtag
    20881 ttatcgcaac aagtggtcct ggtgctagta acttagctac aggtttggta acagcgacag
    20941 cagaaggtga cccagtttta gcaattgccg gacaagtaaa acgaagcgat ctattgaagt
    21001 tgacccatca aagcatggat aatgcagcac tgttccagcc aatcacaaaa tacagtgcag
    21061 aaatccaaga tccagaaaca atttcagaag tgattgccaa tgcttatcgt atggctaaga
    21121 gttcaaaaaa aggcgcgagc tttatcagta tcccgcaaga tgtagtggat gcacctgtcc
    21181 aaggtaatgt catcaagcca ttgagcgatc ctaaattagg ttctgcttct gcagatgata
    21241 tccgttacct agctgaacgc attagagaag caaaactacc ggtactatta gtaggtatgc
    21301 gaggatcaag tgaaaaagaa acgttagcca tcaggcaatt agttgaaaag acagcattgc
    21361 ctgttgtaga gacattccaa gcagctggag tgatctcacg tgaattagaa gcgcatttct
    21421 ttggtcgtgt gggtttattt agaaaccaac caggagatat gcttttgaaa cgaagtgatc
    21481 tagttattgc aattggctat gatccaatcg aatatgaagc aagaaactgg aatgcggaaa
    21541 aagatgcgcg gatcattgtg atcgatgaag cgccagctga aatcgatcct tttatgcagc
    21601 ctgaaagaga attgatcggt gatatctcag ccactcttga tttattgaca ggttcattag
    21661 agcctcaaca agtgtcagaa gatgcaaaag aatatttagc atctcttcaa gcaaaattaa
    21721 cggagcggga cattgttcaa tcaaaaggag aagcaggtat cttgcatcct ttagaagtta
    21781 ttaacacgct tcagtcaaaa gtaacagatg atatgactgt taccgtagat gtaggtagtc
    21841 attatatttg gatggcacgc cactttagaa gttatgaacc tcgtcatttg ttattcagta
    21901 atggtatgca aacgcttggt gttgcattgc catgggcgat ttctgctgca ttggtgcgtc
    21961 caaatacaca gatcgtatca gtttccggcg atgggggatt tttattctct gcccaggatt
    22021 tagaaacggc ggtacgtaaa aaattgaata tcgttcattt gatttggaat gatggacact
    22081 acaatatggt agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt gtagactttg
    22141 gtccagttga ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc cgagctacaa
    22201 gtgtagagga attagaaaaa gctttagaag aaggatttgc gacagaagga cccgtaatta
    22261 ttgatattcc gattgattac cgtgataatg agaaattggg agaaacaatc ttaccagatc
    22321 aattctatta aggaagtgtc gacatgactg aaaaaatctt ataccagcac gggacattag
    22381 gagctttgat ggccggcttg atggatggaa cagaaacgat tgctcatata ttagaaaaag
    22441 gaactctggg attgggaaca ttgcatgggc ttgatggtga agtcatcttt ttagatggcg
    22501 tagcttatca aggaagatca gatggctcag ttgcccaatt agatggaagt gaacttaccc
    22561 cctatgcagc aattacagat tttacgccag atactagttt ttctgtctca aaaactgcag
    22621 atggagaaca attgaaaaaa gacatattag taagagaagc cggggaaaac ctatttttgg
    22681 cagtgaagat aactggatta ttcaaaaata tgcacatccg tatcatgccg aagcaagaaa
    22741 aaccttacag aagactagca aaaatttcag agtctcaacc agaattccaa caaagcaatg
    22801 tccaaggaac attagtcggg ttctttacgc ctgaattatt ccaaggagtt gctgcagcgg
    22861 gatttcacct gcattttatc gatgatacac atacatttgg cggacatgta atggattttg
    22921 aagtggctga aggaaaagtt gagattagcc gcatctcgtc ccttgtacag cattttccag
    22981 ttgatgatcc gacttttgtt cacgaaacga ttgattatgc tgatttagca gcagaaatcc
    23041 aacaagcaga atagaaaaag acagcgagat gcgttcaagg tgattaacct gacgtatctc
    23101 gtttttttga ggagaaaacc tagcgtttac aaaaaaactg agctataatc ttctccatag
    23161 atcaaaaagg agcggttttt atcatgtgga cagaaaaaca gaaaaaagat gcatacgaaa
    23221 ttttattatt acagcaacaa ggattattag aagcagaaga taactggtta gcaaatttag
    23281 caaattcatc tgcattgttg aatgagactt tgcctgagac tgtctttgca ggatattatt
    23341 tattcgatgg taatgaattg attttaggac cgttccaagg gcgagtttct tgcacacgta
    23401 tcaagatggg aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca ttgatcgttg
    23461 atgatgtcaa aacccatgca aattacattt cctgcgattc agccgcacaa tcagaaatcg
    23521 tagtgccaat ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat tgcggagtaa
    23581 cgaaagggta tgatgaaatc gatcaagcat ttctagaaaa atatgtagat cttttattag
    23641 caaattttaa tataaatgct ttttcattta ttaataacta aaatcgtcta ttgaaacaaa
    23701 aaagaagaga aat
//
LOCUS       DASJSZ010000042        23434 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.042, whole genome shotgun sequence.
ACCESSION   DASJSZ010000042 DASJSZ010000000
VERSION     DASJSZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.042"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(270..1766)
                     /gene="lysS"
                     /locus_tag="U4B03_001521"
     CDS             complement(270..1766)
                     /gene="lysS"
                     /locus_tag="U4B03_001521"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEN2068377.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="U4B03_001522"
     CDS             complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="U4B03_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEN2068378.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="U4B03_001523"
     CDS             complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="U4B03_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEN2068379.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="U4B03_001524"
     CDS             complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="U4B03_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEN2068380.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="U4B03_001525"
     CDS             complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="U4B03_001525"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEN2068381.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="U4B03_001526"
     CDS             complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="U4B03_001526"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEN2068382.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8297..8767)
                     /locus_tag="U4B03_001527"
     CDS             complement(8297..8767)
                     /locus_tag="U4B03_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEN2068383.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8813..9238)
                     /locus_tag="U4B03_001528"
     CDS             complement(8813..9238)
                     /locus_tag="U4B03_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEN2068384.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9305..9574)
                     /locus_tag="U4B03_001529"
     CDS             complement(9305..9574)
                     /locus_tag="U4B03_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEN2068385.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9585..11180)
                     /locus_tag="U4B03_001530"
     CDS             complement(9585..11180)
                     /locus_tag="U4B03_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2068386.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="U4B03_001531"
     CDS             complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="U4B03_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEN2068387.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14733..15293)
                     /locus_tag="U4B03_001532"
     CDS             complement(14733..15293)
                     /locus_tag="U4B03_001532"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEN2068388.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15646..16620
                     /locus_tag="U4B03_001533"
     CDS             15646..16620
                     /locus_tag="U4B03_001533"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN2068389.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16790..18190
                     /locus_tag="U4B03_001534"
     CDS             16790..18190
                     /locus_tag="U4B03_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEN2068390.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18268..18990)
                     /locus_tag="U4B03_001535"
     CDS             complement(18268..18990)
                     /locus_tag="U4B03_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2068391.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19069..19575)
                     /locus_tag="U4B03_001536"
     CDS             complement(19069..19575)
                     /locus_tag="U4B03_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2068392.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19698..21497
                     /locus_tag="U4B03_001537"
     CDS             19698..21497
                     /locus_tag="U4B03_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEN2068393.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            21991..23379
                     /gene="ltrA"
                     /locus_tag="U4B03_001538"
     CDS             21991..23379
                     /gene="ltrA"
                     /locus_tag="U4B03_001538"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2068394.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT         6646 a         5056 c         4323 g         7409 t
ORIGIN      
        1 ttttgttatg tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca
       61 actaaattta gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga
      121 ttattcataa cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt
      181 cggcattagc ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct
      241 aaggaattcc ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc
      301 cgaattgatt gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca
      361 ccagttggcg gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct
      421 tcgtcatttc ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt
      481 ggatcgttca gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat
      541 cgatctgtga atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga
      601 tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca
      661 ttgataacat gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa
      721 gctaatgctt cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt
      781 gcatctacca tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat
      841 gtgatttttg ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga
      901 ttcattacat cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga
      961 ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc
     1021 attccgccaa caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca
     1081 atgtccagtg cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg
     1141 tgcaatactg gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg
     1201 ctgataattt ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc
     1261 aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt
     1321 ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg
     1381 tctgttttca tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat
     1441 acttcatagg cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca
     1501 cgatcttgta gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca
     1561 gctacactag cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt
     1621 tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca
     1681 tcttcacgca attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct
     1741 acttgatgtt gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc
     1801 tataatgcca atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc
     1861 acgaaaaact tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa
     1921 attgatccag caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca
     1981 ctttcgctgc tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac
     2041 aagctacttt ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa
     2101 ttttttcggc tggtgttggt tcaggaatca gttcccctgt ttcgagataa tgctgtgtgc
     2161 ggtgaatcat ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga
     2221 catagtcaag catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg
     2281 gaatcgtcaa atgttttttc acttctttca atacttccca gtccgctttt ccttcataca
     2341 tctgaacccg tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta
     2401 atgcattttc tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta
     2461 ctgggatatt tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta
     2521 aaagccattt tgctcctgct tctgctttga ttactttatt tactggacag cccatattga
     2581 tatctattat atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat
     2641 ctttatttcc gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca
     2701 tctctaaagt ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga
     2761 ctaagcccgc accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca
     2821 tcggtgcaac aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca
     2881 cttaaatccc tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt
     2941 atttgttatt acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt
     3001 ggatttcttc tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac
     3061 atttgaattg aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt
     3121 tcaatatttc ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt
     3181 ttaattgttc ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca
     3241 tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag
     3301 ctgaagggat ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag
     3361 aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa
     3421 acattccttg tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa
     3481 gactgagatg cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta
     3541 caattcctgt tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga
     3601 gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt
     3661 gccaagtatc atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg
     3721 ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac
     3781 tcctttttcc gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact
     3841 ttggaatgct tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt
     3901 tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat
     3961 atcagatcaa ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg
     4021 attgttgaga atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt
     4081 cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt
     4141 ttgcttcttc aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt
     4201 caggcatcac accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt
     4261 ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag
     4321 ctttatcatg agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt
     4381 gttccgagta agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt
     4441 catattgaac aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta
     4501 gagctgtcgc ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt
     4561 cttcagctgt acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca
     4621 tcaagaactg atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga
     4681 tgatcgttac tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac
     4741 cagcttcgtg ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag
     4801 ctggacctgc aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt
     4861 tatttcttct agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg
     4921 caaaacctgg tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat
     4981 tccgtgcatg gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa
     5041 tctggcggtc aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg
     5101 ttgcagcaat gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt
     5161 ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac
     5221 ctaccgcatc aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa
     5281 acaaatcacg aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg
     5341 agatcgagta aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac
     5401 ctgttcctgg aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa
     5461 atcggcgagg atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg
     5521 cacccgctac atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg
     5581 atttaccaaa gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca
     5641 tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc
     5701 atataccgct gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt
     5761 ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag
     5821 tcgtatctgt tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt
     5881 tgtattcacc agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct
     5941 tgcctgcttg tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt
     6001 tattaccaaa aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca
     6061 aggcattttt catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat
     6121 ttaatttaac agttttttat caatcctgac cattctattg taccataatt taattgagat
     6181 acagcattaa tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg
     6241 atatgcttct gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc
     6301 aacataatct gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat
     6361 tttgactgat tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc
     6421 gctgtcaatg atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc
     6481 tttgacgatt tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat
     6541 gaaatccaat tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc
     6601 tccttttaag acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt
     6661 gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc
     6721 cttttccaac atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg
     6781 tagtgtattt tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag
     6841 ctatatatat ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt
     6901 actttctgat cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg
     6961 cggtctcctg cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca
     7021 tgagaaaata gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc
     7081 agaccaatcc attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc
     7141 tggtcttctg tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac
     7201 tctcttttca gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc
     7261 cactggtcta aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat
     7321 gctttgtcga aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc
     7381 atttctgcag aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt
     7441 cccatatatt tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa
     7501 tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga
     7561 tttctctgga tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc
     7621 gctgcataag tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc
     7681 gtagcaaatg gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg
     7741 attttcatta acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca
     7801 tattgtcctt cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt
     7861 tctatggcgg atttggctgg atcttcccaa atacttacat ataaagggag attcttctct
     7921 tcacaatatg agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg
     7981 atgtggacga tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc
     8041 aaaaccatcg aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag
     8101 aatccttgtt cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg
     8161 cctttttcaa cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa
     8221 cgtctaaaac gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat
     8281 cattatttgc ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt
     8341 ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat
     8401 cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac
     8461 gatttcggtt gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag
     8521 attcagcagg ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta
     8581 ctgaaataac tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta
     8641 catagccatt tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa
     8701 taaacgcacc aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga
     8761 ttgacataaa aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg
     8821 cagttgtaga actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta
     8881 atataggata aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag
     8941 ctacatactc atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt
     9001 tgacttcttt catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt
     9061 gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa
     9121 ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat
     9181 actttttctt tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt
     9241 ggattcgctc caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc
     9301 taatctattt ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg
     9361 catcttcttt tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc
     9421 caaactggac tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac
     9481 cgttgatttg aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga
     9541 ttcgggaaat ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt
     9601 ttatttaatc gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac
     9661 agacgaagac gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg
     9721 ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca
     9781 taatacagca gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa
     9841 tgccgttctt tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc
     9901 gtcacaccta atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc
     9961 agaaaagaag tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg
    10021 cgaaacgagt tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca
    10081 atggaaaaaa caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa
    10141 ttgatataag ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc
    10201 aatctaagat agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga
    10261 cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt
    10321 actaaaggct gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt
    10381 ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc
    10441 aatccactat aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt
    10501 gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt
    10561 tcatagtagt gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct
    10621 gccgtccctg tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca
    10681 atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag
    10741 aatcttcctt ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag
    10801 gaaactactt ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg
    10861 ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga
    10921 ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg
    10981 aactgcggaa gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc
    11041 tgataaacat aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca
    11101 cttaatactt ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc
    11161 cgcatttctt tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac
    11221 gcaacgcttt gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg
    11281 ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg
    11341 tcgctgtcag tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta
    11401 gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat
    11461 ccattttgat ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac
    11521 ggtcgagcag atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc
    11581 gtttatagat ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat
    11641 cgacaccgag gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac
    11701 gagaaacagc ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc
    11761 catgctgctg agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa
    11821 tcttgaatcc agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga
    11881 cttcgttaag gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc
    11941 ttcctacccg ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa
    12001 cgaacaacgt atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa
    12061 gaacatcata ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca
    12121 tttgcccatg agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt
    12181 tttgttcgat cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt
    12241 ctcgatggat tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg
    12301 gataacgatt cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca
    12361 tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta
    12421 actgtttcaa tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta
    12481 gacccaaatc gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat
    12541 caacttgacc tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac
    12601 gacttaatag cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt
    12661 gctgctgtgc caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg
    12721 ctttaaaggc cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta
    12781 gtcgatccat tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact
    12841 ggtcatcagt ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag
    12901 gtccaaaagc atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag
    12961 cgatatcctc gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta
    13021 gcttattgat gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt
    13081 gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt
    13141 gaacgccatc tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat
    13201 ggacaacgta atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta
    13261 ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag
    13321 ctactatttt ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta
    13381 cctgtcctct gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa
    13441 gcatttgttc tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt
    13501 tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt
    13561 gcatcgtccg atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt
    13621 ggaagagtga gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg
    13681 ctgaaaatgt ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg
    13741 ccgcttccct ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta
    13801 gtaaactatt tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg
    13861 agtagaattt tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga
    13921 agtaatcttg caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta
    13981 gcattttttg catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac
    14041 tcgttgggga aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa
    14101 aataccgcat agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg
    14161 ttaacgggta aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca
    14221 tctcttctcg tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct
    14281 catcaccaat atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt
    14341 tgtgcaaaga tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa
    14401 gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca
    14461 cttcatccac tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg
    14521 ctaactgatt cgcataatac aaatttggca tcagaataat catgttttta tttgttttct
    14581 taaacgctcg agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc
    14641 taggcatttt ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta
    14701 attgaataat atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg
    14761 aacgaatgcc cttctatgag atattcgact gctttaacag attgacacat gctttcttct
    14821 atcaaaggct gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc
    14881 ccttctggcc tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa
    14941 ataatacttt tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt
    15001 cccactgcca gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa
    15061 taagtcatta gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact
    15121 aaaaggatct tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt
    15181 ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt
    15241 tttgtatatt ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt
    15301 ttccacctaa ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca
    15361 tcgagctttt ttctttttta cacttgtcaa tgcatagtat aacacaaaac aaggcgtttc
    15421 ggctaaaaga gaatcgttaa taaatgataa agaaattaaa aatttcttca aagtttaatg
    15481 aaataattga tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca
    15541 accttgttat ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta
    15601 gcataagcat gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca
    15661 gaaaaaaaag atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat
    15721 gcctttgctc tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact
    15781 gctaaaactg aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa
    15841 aaaatctatt ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct
    15901 ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc
    15961 aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc
    16021 gctaacccag tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa
    16081 cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa
    16141 ttagttgacg ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca
    16201 gaattcccag tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa
    16261 aacaacccag aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct
    16321 gcttacacaa ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca
    16381 cgtatcactc gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg
    16441 aatggcgaat atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa
    16501 ggtatccaaa aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct
    16561 tctgctaagc aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa
    16621 tctttgaaca tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct
    16681 tatggtttct aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg
    16741 ctatcatgag accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc
    16801 ttcgcatcga aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt
    16861 gctgatcagc ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac
    16921 agaaataaat ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa
    16981 agaagcttat tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt
    17041 accaaaatcc gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa
    17101 tgaacaaaac ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat
    17161 agatcctgtc agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa
    17221 gctgactgat ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg
    17281 tgactctttc gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt
    17341 caaacaatta aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa
    17401 agaaaaacca aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa
    17461 tgctatcgca aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc
    17521 aagtgatatt atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca
    17581 agtacgtcaa tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa
    17641 atttaaaagt acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat
    17701 ccaaacagac agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac
    17761 tcgttcaccc attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta
    17821 tatcgaagtt gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct
    17881 tgaaacagat atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt
    17941 ttggaataaa gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga
    18001 gagtccggta aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga
    18061 ctggcaacct gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa
    18121 caccccacca agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt
    18181 ggtcttctaa caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt
    18241 tctttttatt atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac
    18301 atcatcatcg tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt
    18361 taaatcaatc gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt
    18421 ttccaactca tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc
    18481 aggagaagtt tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc
    18541 atcaacagtc aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact
    18601 gactgaaccc gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt
    18661 cgctgtccga ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc
    18721 atatccttca tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt
    18781 aatcgcccgt tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag
    18841 ccgcaaagct ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc
    18901 acgagaaagt ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg
    18961 gatattatgc catttactat gtcctgacat gatttttcct cccttgttct tttgagaata
    19021 cacttctatt ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt
    19081 cttgaatgcc gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa
    19141 catgacatct tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa
    19201 acctgcgtac cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag
    19261 cttggggaaa acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc
    19321 gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc agatggttga tactcaccat
    19381 acttccacca taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag
    19441 ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa
    19501 taagattata acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt
    19561 ttttgaagaa ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac
    19621 aatcattttc atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat
    19681 atgaagggag ttttttcatg agttattcat taaattggaa tctagattct attttttccg
    19741 gaggcagtca ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg
    19801 agtattatca ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg
    19861 ctattcttca attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta
    19921 ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt
    19981 acgcaatgct gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa
    20041 tatctgacaa tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc
    20101 gtttaaacga gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta
    20161 taaatacgct ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag
    20221 ccagcatttc gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag
    20281 ccttcaataa gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat
    20341 gggaagaagc ctggaaagaa aaggcacccc tcttcacgga tactttgaat catttagatg
    20401 gttttagact ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag
    20461 agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca
    20521 aacaacccct tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg
    20581 aatggcaaga tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt
    20641 ttgacgaagc agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta
    20701 aatttgccca atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac
    20761 gtcctggtgg ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct
    20821 acagtaattc tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata
    20881 gcagcacgat gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa
    20941 cagcaagtac attcgcagaa ttgatcgtag ccgatgctac attaaaagtc gcaaaaacaa
    21001 aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga
    21061 atatccattc ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag
    21121 tctcagaaga taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag
    21181 ctttagctac ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg
    21241 taccgtttta taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt
    21301 acgccaacca aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg
    21361 cctcaatgac gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag
    21421 atttttggca agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga
    21481 cagaagaatt tatctgattt ctattttatc acaataaaaa aagagttagt ccagaatggg
    21541 ctaactcttt ttgatgtgcg ccctgcatgg gtgaaaactt ggtggtgaaa gtccactaca
    21601 ggcttggcag taggaactgt tagcgaaaga caaggcggca atcgtgaggt tgtggctgaa
    21661 ggaagtcgga cgcaaactcc tgaactgacg aacagaaact agatacaagg ctgaattagg
    21721 tcggataagt cttcatcaca agacaaagtc caatactgcc cgaaacctat acagtaaatc
    21781 tagcagttac atgggaggaa agttttcact cttaccgggg gagatctcat gagggtgttg
    21841 tgggcgttga atgaaaacaa accagcacaa cgatcatggc gacatgatgg tgaatcatga
    21901 gaagtcagcc gaggtcatag tagtaacgac actgaccgtt acgaaggact gaacaataag
    21961 aatcttgaat aatttaggag gtgtgagaag atgcgacgac cgcagaaaac aagaaaactt
    22021 ggctatcatc aaagagctag tgtggaacac gaagggtatg atgaagtgcg cagcacttct
    22081 tgcggtgaac agaggaaaca agatggtgta gaaaatctgt ttgggagaat tctagatcgg
    22141 aataatctca atcaagcgta tcttcaagta gtcagaaata aaggggcggc tggtgcagat
    22201 gggatgaccg tcgatcaact gcttccttac ttgaaagagc accgcgagga attactatcc
    22261 aatttacgcc aagggaacta taaacctcag cctgttttgc gagtagaaat accgaaacca
    22321 aacggcggtg taagaaaact aggaattccc acggttattg atcgtatgct tcaacaggca
    22381 atcaatcaag tgttacaacc gctatttgaa ccagaattct cagataatag ttatgggttt
    22441 cgcccaaatc gcagcgcaca tgacgcagtg aagaaagcaa aatcttatta tgaacaaggt
    22501 taccgatatg tggtggacat tgatatgaaa gcctactttg acaccgtaaa tcacgataag
    22561 ctgatgtatt tcattgagcg gaaagtaaag gacaaacaag tcctaaaact gatccgtcaa
    22621 tacttaatga gtggtgtaat ggaaaatgga ctcatcacaa caactgaaga aggaacgccc
    22681 caaggaggca atctctctcc cctgttaagt aacatctacc tgaacgaatt tgatcaagtg
    22741 ctagaaaatc taggacataa atttgttcga tacgccgatg actgcaatat ctatgttaga
    22801 agcaaacgag caggacagcg tgtgatgaag aaatccattc aatttctcga agaacaacta
    22861 aagttgacgg taaatcgaga aaagagtgcg gtaggaagtc cattgaaacg aaagtttctt
    22921 ggattctgca ttctgccaac aaagaaaggt attaaaattc gccctcacgc aaaagcaaaa
    22981 cagcgtgtga aaagcaagct caaacaactc actaaaagaa accgtggaag aagtatccaa
    23041 cttattttaa aagaaatcaa gcaactaatg actggttgga tcaactacta tggaattggt
    23101 gagatgaaag aattcatgag ggagttaaac ggttggttga agcgaagaat cagacaatat
    23161 atttggaaac aatggaagaa tccaagaacc aaaagaaaaa atctgattcg tttagggatc
    23221 gataaagcga aagcgtatga atggtcaaat acaagaaaag gtatttggtc agtctccaaa
    23281 agtcatattc ttcatcgatc attaacagac aaagaactgg cccgccaagg atatgaggac
    23341 atctccttga agtaccagtt cgtacactca acttattgaa ccgccgtata cgggtccgta
    23401 cgtacggtgg tgtgagaggg gcggaaggtc agtt
//
LOCUS       DASJSZ010000043        23101 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.043, whole genome shotgun sequence.
ACCESSION   DASJSZ010000043 DASJSZ010000000
VERSION     DASJSZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.043"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            20..541
                     /locus_tag="U4B03_001539"
     CDS             20..541
                     /locus_tag="U4B03_001539"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2068395.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            542..859
                     /locus_tag="U4B03_001540"
     CDS             542..859
                     /locus_tag="U4B03_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2068396.1"
                     /translation="MKEARPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(1016..1180)
                     /locus_tag="U4B03_001541"
     CDS             complement(1016..1180)
                     /locus_tag="U4B03_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEN2068397.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1283..1804
                     /locus_tag="U4B03_001542"
     CDS             1283..1804
                     /locus_tag="U4B03_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEN2068398.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1791..2264
                     /locus_tag="U4B03_001543"
     CDS             1791..2264
                     /locus_tag="U4B03_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN2068399.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(2271..2429)
                     /locus_tag="U4B03_001544"
     CDS             complement(2271..2429)
                     /locus_tag="U4B03_001544"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEN2068400.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2522..3283
                     /locus_tag="U4B03_001545"
     CDS             2522..3283
                     /locus_tag="U4B03_001545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2068401.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3503..4822
                     /locus_tag="U4B03_001546"
     CDS             3503..4822
                     /locus_tag="U4B03_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2068402.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            4838..6058
                     /locus_tag="U4B03_001547"
     CDS             4838..6058
                     /locus_tag="U4B03_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2068403.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6093..6770
                     /locus_tag="U4B03_001548"
     CDS             6093..6770
                     /locus_tag="U4B03_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN2068404.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6767..7963
                     /locus_tag="U4B03_001549"
     CDS             6767..7963
                     /locus_tag="U4B03_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2068405.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7978..8301
                     /locus_tag="U4B03_001550"
     CDS             7978..8301
                     /locus_tag="U4B03_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN2068406.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8305..8640
                     /locus_tag="U4B03_001551"
     CDS             8305..8640
                     /locus_tag="U4B03_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2068407.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8637..9032
                     /locus_tag="U4B03_001552"
     CDS             8637..9032
                     /locus_tag="U4B03_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068408.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9029..9400
                     /locus_tag="U4B03_001553"
     CDS             9029..9400
                     /locus_tag="U4B03_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068409.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9416..9976
                     /locus_tag="U4B03_001554"
     CDS             9416..9976
                     /locus_tag="U4B03_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2068410.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10103..10414
                     /locus_tag="U4B03_001555"
     CDS             10103..10414
                     /locus_tag="U4B03_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068411.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10438..10629
                     /locus_tag="U4B03_001556"
     CDS             10438..10629
                     /locus_tag="U4B03_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068412.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10629..14474
                     /locus_tag="U4B03_001557"
     CDS             10629..14474
                     /locus_tag="U4B03_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEN2068413.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14475..15347
                     /locus_tag="U4B03_001558"
     CDS             14475..15347
                     /locus_tag="U4B03_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEN2068414.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15344..16393
                     /locus_tag="U4B03_001559"
     CDS             15344..16393
                     /locus_tag="U4B03_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068415.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16397..17221
                     /locus_tag="U4B03_001560"
     CDS             16397..17221
                     /locus_tag="U4B03_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068416.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17239..19371
                     /locus_tag="U4B03_001561"
     CDS             17239..19371
                     /locus_tag="U4B03_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
                     /protein_id="HEN2068417.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19388..19537
                     /locus_tag="U4B03_001562"
     CDS             19388..19537
                     /locus_tag="U4B03_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068418.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19534..19980
                     /locus_tag="U4B03_001563"
     CDS             19534..19980
                     /locus_tag="U4B03_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068419.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19982..20119
                     /locus_tag="U4B03_001564"
     CDS             19982..20119
                     /locus_tag="U4B03_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN2068420.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20156..20449
                     /locus_tag="U4B03_001565"
     CDS             20156..20449
                     /locus_tag="U4B03_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068421.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20472..20669
                     /locus_tag="U4B03_001566"
     CDS             20472..20669
                     /locus_tag="U4B03_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN2068422.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20680..21699
                     /locus_tag="U4B03_001567"
     CDS             20680..21699
                     /locus_tag="U4B03_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN2068423.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21956..22603
                     /locus_tag="U4B03_001568"
     CDS             21956..22603
                     /locus_tag="U4B03_001568"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2068424.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(22928..23074)
                     /locus_tag="U4B03_001569"
                     /pseudo
     CDS             complement(22928..23074)
                     /locus_tag="U4B03_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         8036 a         3916 c         5096 g         6053 t
ORIGIN      
        1 ggattaagga ggaagagtaa tgcatatagg agataagata cataaattga gacggcttaa
       61 taacgaaaca atgaacgact tagttaaaca aaccggactg tcaaaatcat gggtatctga
      121 tattgagtct ggaaaaaagc aacgcgctga ctttaataag atatgtgcaa tagccaaaca
      181 ttataaagtt agcttagaat actttcagga tgattatttg gatagtgatg aacacgagga
      241 tattattcct ttgacagacg aaacaagagt taaatcagtg aaatcgcttg aacaaagcct
      301 aaagtatctg aataacaaac aagaacctaa ccttctagag atcaaactaa aagatactga
      361 ctcagtacca gaggtttatt acaaaggtca gaagttagag ggattgatag atgtaagcta
      421 tcactaccat acaacaactg atgtggtagc tggttcaaca aacgacatca atatggagta
      481 cctaggtgaa gtaactaagt atccagatat aaaaacaatc ggacacaaga gagatgtgta
      541 aatgaaagaa gctagaccta gagacgagat agacaaacta tacaagacca aacgatggcg
      601 agacctaagg caagtagtaa tagctaggga cttcggcatg tgccaagagt gcaagcgtcg
      661 agggcggaac acaaggggca cgatcatcca tcacatagtc gaggcgaggg aagacctgtc
      721 actgttctgg tccgtagata accttgaatg tatctgtgta gcttgtcaca acagagagca
      781 tccagagaga tcaggcggga agaagaaacc aaaacctaaa tcacatatcg ttaaaatgta
      841 ttcaactcct gaaagataag tttgcagcga aatgaaggta gcccccctac tctaaaagat
      901 taaagagtaa ggcttgagaa gaacggtgct gtccttcctt cgtaaaaaga ccgcttttca
      961 agttttttgg agaaaaagga aaaagccgac caatttaagc cggctttgga cgaagctatt
     1021 tcttagtcca tttgtttcct ttttgagaag taggaggtaa tcggtcgcct ggatcaattt
     1081 ttacttctcg tccgccttgg acatttccac cacgaggtcc cacttcttta taggttcctt
     1141 ttggtttatt gtcttcgccg ggtttataga gttctcccat aggaatccct ccctaaaaaa
     1201 atttcggcac agcactgccg ataacttaat tataaggatt gtgataacga tttcaatata
     1261 tcttttgaaa gaaggtgata ttatgccgca accagcgaag agtgcaaaat tacaattatt
     1321 aaacggaaac ccaaataaga agaataccga agaactccgc aagcgagcgg ccgcagaaga
     1381 caaattaaaa atggctactg acaaaatcaa accgccgtta tggctagatt cgctaggaaa
     1441 ggataccttt gagtttatcg ctgatgaatt gctgtctgtg gatttaatca gtaatccgga
     1501 cgtccataca atggctctct actccaattg gtattcgcaa tacgtttctt tagaaaaaca
     1561 gcttcgaaaa ctacaacgag agtacaagtt gaactatgcg cttgcgaaaa aggaggcaga
     1621 ggcgagaggt gagccgttta atgaacctaa tgaattaatt ggtaatccgc tctctcggca
     1681 gatggataca gcgtcgcgga atctccgttc ttttggcgct gatttaggac tatcaccagc
     1741 agccagagct aagttagcta ttaagatggc tgatgatggt ggtgatgacg atgacgactt
     1801 ctaatatttt ggatatgtcc tacacagaac gtgtggacta ttggcaaagc tatcttgagg
     1861 agcaagcttc ttggggtggc tttttaaaat gtccatatcc ggaattgtta actacttggt
     1921 atgcggaacg attaatcgat ggaagcatac cagccagcaa agaaaatatt caagctgcta
     1981 aacggcatat gcgtgatttg cagcgccaag gaacagatga ttttccttgg atctttgacg
     2041 aagaaaaagg tcaccggcct attagatata tcgaaaaaaa atgtaaacca actgaaggcg
     2101 actttggttc gtttgttttg caaccttggc agcatttcat aattggatcc atgtacggat
     2161 gggtacatcg tgatacagga gagcgtcgct tccgcgaggc tcttattttt gttggacgta
     2221 aaaacggtaa gttttgccgt ttaaaaatcg ggcaaaatcg gtgaaaacct ttatttttgg
     2281 ctttctttta gtaataattc gatagcttta tcaagcaatt tagacatagg aaccatagtt
     2341 tcttccgaca ttttttttaa tccttcgtat aaatctttat caattgcgtt agaaattcta
     2401 attctgtttt ttagtccgta tttgtccatg tgtgacactc ctttttattt tactataaca
     2461 tccgattcca ccgcttgcaa ctaccatcaa ttgatggtag tatataaaag aggtgagtag
     2521 attgaaaaaa tacattattt acaaacactt aaataaaata aatggcaaaa tttatatcgg
     2581 tgtcacaaat gatattggca gaaggtggag gagcggtggg attgaatata aaccaccaaa
     2641 gaacgaaacc caacaccaca gaagtttttg gaatgcaata caaaaatacg ggtgggaaaa
     2701 ttttgatcat ttgattattg aagaagactt aacgatgaaa gaagcttttg agaaagaaaa
     2761 gttttatatt gaactttatg actcaactaa taagaaaaaa ggttataaca tcgcaaaagg
     2821 cggaaatggc ggaataatat ataaagttca ccccaaagga atgctaggga aaaaacaatc
     2881 taaagaattt tcttcaaatc acagtaaatg ggcaaagaac cataagaata attgtatgac
     2941 aaacggtgat gttgtatggg gtgttactca cgaacaccca aaaggcatgt tgggaaagca
     3001 ccacagcaaa gaatcaatta tgaaaaagaa agcatatagt ggtgaaaatg ctgtaacctc
     3061 aaaaccaatt gtcgcaattg aacgggatgg aactaaaaga gaatttcaca gcgctaagct
     3121 ttgcatggct tactattcga ttagtacatg tgttttttac cgacttttaa aagatggcgc
     3181 cccttatgtg ataaatccta aagcgaacta cagaaataaa gaaaaaatat tagctatcga
     3241 aggaattatg ttcaaacata aagaagatac cgaggtaagt tagtagattg cgaaaggtta
     3301 ctaactaccg taacgcgtag gagttgaata aatataatgc tcccaagagt gtccgacagc
     3361 agataatttg ctgaaaatgt acgctaaact gggccagaac tgactgaccg atgaaaatga
     3421 gggtgacctc cagagtgcga gataaaaagc tcgcagataa taacaatcga aaacatcact
     3481 aatttcaggc ctttccacat acatggtcgc ttatgatgat gaacaaggcg ccaacgttta
     3541 cgtattggca aatgctcgtg atcaagcaag cttgttgttt gataaggccg cagaaatggt
     3601 caaacaatcg ccggcgctct ttaagaaatt tggtaagcct aaacgatcaa gtattaatta
     3661 tgctcccgcc ttttctaaaa tggaaccacg cgcctcagat agccggaaat tggatgggct
     3721 aaacactcac tttggtattt ttgacgagat ccacgagttt acgaattaca atctgatcaa
     3781 cgttatcaag aaatcaagag gaaccagaaa acagcctctg atagtttata tcacaactgc
     3841 tggatatgta ttagatggtc cgttgatgtc ttattttgag caaggtgtgg actgtttgga
     3901 acatttggaa gatgacatcg atgaacggac tttctattat ctggcaaaac ttgacagtgc
     3961 ggaagaggct gatgacccaa gattatggat caaagccaat ccgaatattt gtctaatgaa
     4021 ttttgttggc atgctagatg actatgttaa ggataaaaaa gatccaaaag aatatgctga
     4081 ctggattacc aagcaattta acttgttttc cgatatcgat gagctgtcat ttgtcgatat
     4141 gcctaccatt aaacgaaaca ataaaaccat cgatattgaa acgctcaaag gtaagaagtg
     4201 tgtcggtggt tttgacttgt ccgaaacgga agactttacc gcagccgttt tagaatttcc
     4261 gcttgaaaca ggcgaggtat tcattttgca acacacatgg atcccacaag ctagatttga
     4321 tcgagataac aatcaagagc gtatcaaagc gtgggagaag gtgggagatc taacgattat
     4381 tcctggtgat tacgtcaatt atgaatacgt cttaaattgg tttgtagaaa attcgaaaat
     4441 ctatgacatt gtaaaaatca attatgacaa ggccaaggcg ctacgattaa ataaagaact
     4501 agaaaatgca ggatttgaaa ccaacgagat tcggcaaggg tttctatcat taggtgggcc
     4561 aatgcaaaac ttcaaggaaa tgctattgga cggtaaggtg attttcaaca attccaagct
     4621 ttaccgatgg tatctatcca acgtcaagct ggtgatggat cgcaactcaa actggatgcc
     4681 gtctaagcag tccaagagta gaaaaataga tggttttgca gcaagtttga acagccacgc
     4741 cgaagtgttg aatatgttgg ttaatcctgt cggaaccggg aaagtaacct attactcgat
     4801 ttccgattta atgaatatgt aagaaaggtg tggaggaatg agtattttag atcgtttgcg
     4861 ttcttttggc cgagcgaagc cgaaagcgag caaacaagag tattttttga atgacccggg
     4921 attgataccg tatttagtcg gaaaagatga aatatcagaa gggatttttt ccgtaattag
     4981 ccgtgtatcg aacgtttttg cgtctctccc tctcaaaatg atagatgcgg agtttggcca
     5041 accggacgac tgtcctgcat acaacttgtt gagcgaaggc cctcgatatt ttacaaagtt
     5101 tgattttttc cgggacgtgg aagttttgag aaactaccaa gggaatgcgt atgtgcagat
     5161 tttccgaaat atcaatggag aagtagcaga tatggcgtta ataaaacctg gtgcttgcca
     5221 tccagtgatt gatatggata gcggggagct ttactaccaa gtaactgcga ctgacaaagg
     5281 cagttataag caagttattt atgtacatta catggaaatg ctccacttta aacaaccgag
     5341 gtttggtggc ttggaaggta cagaccccac aaaagtatta acgaataccc tcggatatga
     5401 tcgagaagtc cgaaaaatct ctttaagtca gcttaaagga ggtaatgaag ggctaaaagt
     5461 taagtttgct agcaatgtgg atgaagaagc taaaaaagct acagttaaaa acattgctga
     5521 tttttatcga caaaacggtg gactacttgt ggaagaaaac ggtgtagaaa tcgaacgttt
     5581 acaacgagag ctggtagaca gcaagctttt agatactgat aaagtatctc gctccagaat
     5641 cgcgatggtc tacaacgtgc cggaacattt cattgggagt aaccagtcga gttactcttc
     5701 acaggaacag ctcaatatgg agtttttgac atacaatcta gtaccgaccg ttaatcaata
     5761 tgaagcggaa ctaaataaga aaacactatc gagaaccgaa aaagcaaaag gttatcgata
     5821 taagtttaat gtcgcaagtt tgctaagagc tgacactcag gcaagggggc aattctatca
     5881 aatcatgcga cgaggcggag catactcagc gaatgatgtc cgaagatttg aggacttgca
     5941 accgataaat aaaaccggta tggatgatta tcatatttcc ggagacctat atccaatcga
     6001 tatggatcca acattaagaa aaacaaactc gtctaaaagc gtagccgaaa acggttaggc
     6061 ttttttagtt tgcaccgaag ggaggtgaaa ggatgaaaaa agtgacgtta agcggcgatg
     6121 tcgtggataa cgataccgcg tggttatatg actggtttgg gattgattgt atctcaccag
     6181 ggaaaatttc tgccgctctt acagaagcag cgggggatga agtagaactt gatatctcat
     6241 ctaacggtgg ggatgtccta gcggcaagcg aaatatatac cgctatccgt gcctatccgg
     6301 gaaaggtatc cggaaatgtt gtgagtattg cagcaagcgc tgcaagtgta atcgcttgtg
     6361 cttgcgaacc gcttagaatc tcgccaacag cacacatcat gattcacaac gcatgggtga
     6421 ccactagcgg caacgccgag gaattaaaag ccaatgcgga aatgttaagc agtgtggatg
     6481 agtctatcgt taatgcttac gagatcaaga caggactcga ccggaaaaag cttgctgatt
     6541 taatggcaaa agatacttgg ttaaacgcac aaacagcagt ggcggaaggt tttgcggatg
     6601 aaattatgtt tgcagaagca ccagtaacgg tactcaatgc ctctcaaccg gttattccaa
     6661 aaaacgcggt aactaagttg aaaaatctaa tactcaaagc ggaaacaccg caagaagaaa
     6721 cgctcttgca gaaaaaacta aaagccttaa atggagggaa aaacgaatga atttagaaca
     6781 attaaaaaat gcgtgggtcg aggcgggaag taaagtctct gatttaaacg cgcaactcaa
     6841 tgcagcattg gttgacgatg aaaaaacaga agaagatgta gtaagtttgc aagcacaagt
     6901 aaaagcagca cgggctaaac gggacggatt gaaagagcaa gtggcaaata tggaagccga
     6961 acaagtctta aacgtcaaaa aagaaccatt agataaaaaa gatgaaaact tgaaaaacaa
     7021 gtttatcaaa gactttaaag cgatggtcaa tggtgatcct gctattatgg ctactttgac
     7081 atctaatacg gatgaatctg gtaatgctat cggattgact attcctgtag atgtgcaaac
     7141 gactattcat actttggttc gtcagtttga ctctttgcaa gaatacgtaa acgttgaaaa
     7201 agtgaccact actagcggtt ctcgggttta tgaaaaatgg tctgatatta acccactgac
     7261 cgctttggat actgaagacg gtgaaatccc agcaaatgat gatcctgcac tttacttgat
     7321 caaatacttg atcaaacgct acgcaggtat ttctacagta actaacagct tgctaaaaga
     7381 tactgccgaa aacattttgg catggttgtc taactggatc gcgaaaaaag tagttgttac
     7441 tcgcaataca aaaatcttgg cagctattga tggaatcaaa gcagcgcaaa agaaagatgt
     7501 tacagatgtt gatgggatta aagatatcat aaacgtccaa cttgatccag ctatcgaagc
     7561 tacatctatg tttattacaa accaagatgg cttcaatgtt ttagataaag tgaaacgtgc
     7621 tgatggatct tacttgttac aaaaagacgt agcttctgca actggatata ccttcttagg
     7681 taaaccaatc aagaaaattg cttctcgttt cttgccaaac aaaggtacgc aagctactcc
     7741 taaatatcca ctgtatattg gtgatctgaa agaagctgtt acattgtatg atcgcgaaaa
     7801 catgagcttg ctgacaacga atattggcgg gggggctttt gaaacagaca ccaccaaagt
     7861 acgggttatc gatcgatttg atgtgcagtt agtcgatgat gaagcagttg ttttagctac
     7921 atttacatct atcgcaaacg aagtgccagt aacaaacggt taaggagctg atttcttatg
     7981 attcttgatc ctaaaatgga tttagacgaa atcaaaaacg cactaaagat tgataccgat
     8041 gatgacgatg tggaagtaag acgtgcggtg caagctgcaa ttgcatacat tagaggggct
     8101 atcgggaatg ataagccctc tttttataag caagaaaatg acacggttga tctgattaat
     8161 ttagctattc tgcaattagc agatcattat tacaacgctc gatcagcaac cgtgagtggg
     8221 aacttgcgag agtacgattt aggttttaca agcctaatct tgcaactcaa agcaagatat
     8281 ttgctttttg tggaggagta gcgtatgccc cttattcaaa caggaaattt aaatcaacgc
     8341 atcaagtttg tccgagatac gacggtcaag gacgaggacg ggcaagttgt ccggacttct
     8401 acaaccattc ttacttgctg ggcaagtgtg cagacacaac gcctgaacga tattaagacg
     8461 tccattggta cggctttgga aggaacactg acgttcatta tccgctatca acaaaaatca
     8521 gagctaacca atgatatgaa agtgcgttgg aatggaaaaa cgtttgaaat cattacgatt
     8581 acgaaaggcg agtttgcgaa ggacttcacg acaatcattg caaaagaggt ttcaaaatga
     8641 gtgtagaagt cgatgcaacc gaagtgtaca aagcgcttag ggaagtaaaa gcaaacgttc
     8701 aacgagtgga aagcccagca cttagaaaag ctggggagta cgctcaagaa aagttacaac
     8761 aaaacacacc ttactgggat ggaacgaagt caaacggtaa acgtggttcg tatatgcaag
     8821 aacatgctaa ggaccatgtg gttacaagct cggtaaaaaa cggattgata gaagtcggct
     8881 atgataaaga tgtttcttgg cggatgcact ttatcgagtt cggaacaatc aaacaacgtc
     8941 caaaaggttt cgtacaaaaa acacaaaagc aaatcgaaaa acaagtaaca caaatcattg
     9001 ctgacgaagt aaaaaggagg ctaggacttt gaaaacggca gtatcacaag tctattcaat
     9061 tctgaatagc aatgaaaaaa caaagaacat tgatttttac accaatagtg ttccggaatc
     9121 ggctcaaaca gtacctagcc ttccagttgg cagaattaca gagatattcg gcaactatga
     9181 agatttcgca agcaacaatc ctttgaccat tcaatttaac gtacaggtag atgtatgggt
     9241 atcgacctta aaagaggttg atgcctttta ttttgccctt gatgagatta tgagggggaa
     9301 tggttggcaa tgcgcataca cggaacaaac agatgatgag gatttagaag gtgcaaagcg
     9361 gattatcaaa cgatatgtag caaatatttc actaaattaa aaggagagaa aatagatggc
     9421 aacagtagga tttgagagcg tcatttttgg cgtaaaaaca ggtgcaggcg gcactctaaa
     9481 agaattagta gcagataagt cgaaaggcgg agcgatcgaa gctaaaatta ctggattagg
     9541 cgcaacttct aacacaacat acgcttcaaa cgtaccattc ttcattgcaa gtaaaggggt
     9601 ttcgtcgcca aaagttacgc ttgacgtggc agacttaatg gataacggca tttacagcga
     9661 aatcattggc gctaaaaccg tggatggtgt aaatgtaatt ggttcagaaa ctgaagcgcc
     9721 ttacgtgtcg gtagtcatgg ttacagcgaa caaagaagga aaacgcttat tcatgggatt
     9781 ggcaaaagga aaattcagtc atccagatat cgacatgaaa acagctgaag acaaaggagt
     9841 tgaattgcaa accgattcta tcgaagggga attcatttct gatgaacgtg gctatgtata
     9901 cttaacagcc gtagaatcag aagaaatgac cttacaaaaa ttcaaggact tggtaaataa
     9961 caaagcgggg gagtagttaa ccctgcatct acaccaacga cagatacagg gacaccaaaa
    10021 gaaccagaac caaaaattga tacacaaggt tagccatttt ttggctagcc ttattttttt
    10081 gtaaaaacaa ggaggaaaac aaatgattga attgcaattg aaacttgacg gaaagaaaaa
    10141 aacattcaaa caacaagata tttccgcacg tgcaatgcgt gagtgtatca aattttacga
    10201 gaaagcggaa aaagcagacc taactgattt agaagcaatt gattcaatga ttgcaattac
    10261 agcagatatt ttccaagatc cagcagttac atttgatgct attttagacg gtttgactgc
    10321 gagcgagtta gtaccggcat tagaaagtgt ttttgaacaa atcaatgaac tgggaaacaa
    10381 tgaaaaaaag cagacggcga gcaaaaagag ataagttttt ctgaagctag gaaagcaatg
    10441 gatcaaatct acaaagattt aatcgaatca ggttggacga tgagagatgt ggacgaagcc
    10501 gactatcatt atttgttaca cctttttgga gaagtggaga gtggcgaaga atatgtagat
    10561 ggtgctgatt tcatcaaaca atttttatcg gctgaagact tggtaaaact tgaggaagga
    10621 ggtaaataat ggcaggaaaa ggacaaccgg caggaaatat caagctaggg attagtttag
    10681 atagcactaa ttttggtaac acgctggacg aaatcaatgc gaaagtcaaa caagctgagt
    10741 cgaatatgcg tgccaatcta aaggcttatg attcagcagg acgttcatac gaagcactta
    10801 gtcaaaagac gaaagacttg tctacggtta tggaagggca aaacgccaaa gtaagagaat
    10861 taacaaagcg ccgtgatgaa gcgattagca agtatggcga ggaatcgaaa caagttgcta
    10921 accttaacac acagataaac aatgctaccg caaaatataa tgcttacagt cgccagttga
    10981 atgacacaaa aaaagaattg gtgtattcca aaacagccgt caatgattta tctaacgaaa
    11041 tcaaagaaaa tgaacgacaa atgaacgccg aagtaaaagc gttgaaagcc gctggtgatg
    11101 aatctggtgc gtttgaagca aaacaaaaag ggctagccaa acaaacggaa ttatccgaga
    11161 aagctatcga agaacagcgc aaagttgtga aactgatggc tgatgagttt ggcgattcag
    11221 caaatgaaac cgaagatgca aaaagggcat tagaaaagtt agaacgacaa agccaaatat
    11281 ctagcaggca attagaagca ctcaaaagct ccagcgatca atcaggaaaa aaaatagaag
    11341 attttggcga caagtccaca aggtcagcta ggaaactgga tgggctaaaa gacaaattag
    11401 gctcgctaaa aagcgcattt tcgtttggtg cagttgctgg attagcgcat aacgctatta
    11461 gcagtgtagt aagtggcgtg caaggcttgg ttggcgaagc agtaaacgca tcggattcat
    11521 tgatgaagtt ttccaaaacc atggagtttg ctaactttgg gaagtcacag atagaaagct
    11581 cgaaaaaaga aatgaaagac tacgccgata agacggttta tggtttagaa gaaattctga
    11641 acacaaccgc acaattggca tctaacggga ttcctaacta tacagaacta accaaggcgg
    11701 caggtaactt gaatgccgtt gcaggcggtt ctagtgatac attcaaatcc gttgccatga
    11761 tgctaacgca gacggcagga gctgggaaac taacaactga aaactggaat caattagcag
    11821 atgcgatacc gggcgcttca gggctgttac aagacgctat gttgaaaaac ggagcttata
    11881 caggaaactt ccgtgatgca atggcgcaag gtcaaatcac ttctgatgag tttaaccaag
    11941 cgattgtaca gttaggtatg aatgacggag cagttaaagc agctacttcc acagatacat
    12001 tgagtggttc ttgggaacag atgaaatcta ctgtaataaa tgggttacaa agtatcatag
    12061 acaaagtagg cgttgaaaat atcactggtt tcatcaacag agtaacaaaa gggattgaaa
    12121 attctattcc taaaattact caatttatag gttggttgag agatattgga acgtggatcg
    12181 ttgaaaatag agagccacta acatggattg tcggaatcat aggcggaatt acattagcag
    12241 taaaagcatt gaacgtagca agtatgttgc tggcaattac tggcggaaca ttggcagccc
    12301 cttttgtggc gattggtgta gcattaggcg cactagcagg tgctttggtg atagcttata
    12361 caaaatctga aacatttaga aatatagtca atgcggcttt tacagctgtg aaaaacgtag
    12421 ttatgagcgt tatcaataat ttggtggaat actacaaaat gttgtggagc gtgttgcagt
    12481 ggctttggga aaaaataaaa gaatgggctt catggattgg caataaattc attgaaatga
    12541 agaacagcgt tgtgaatacg gttcagaacc tatggaatgg tgtgaaaaac ttcttcagta
    12601 atggcgttgg agacacttgg aataaggtag ttggctgggt aaaaaacatt ttcaacaaag
    12661 caactgaatt gaagaacaaa gtttctgatg caattggtaa cctgtggaac ggtatcaaag
    12721 acacattccg tagaggcatt gatacggtat tcaattggtt ttcagaacta ccgacgaaga
    12781 tgaagaatgc cattattggc ggtaaaaatg ccattgttga tgcgttcaaa agtattttca
    12841 acgcagcact taaagcgata ggtaaaccag ttaacgcaat catccatgga gcttcatggg
    12901 tactagaaaa actgggtgct gacaaactcg aagaatggaa agtgccacaa tacgcaaaag
    12961 gaacaccaaa cggaggtcat ccgggcgggc ctatgatggt aaatgacggt agaggtgctg
    13021 aagcggtaat cacacctaac ggacaagcat ttatcccacg agggcgaaat gtagtgttga
    13081 atgcaccaaa aggcacacac gttctaacag ctgaagaaac agcttatatg actggaaaca
    13141 aagcaccaag atatagatac gccaaaggta caggcttttt cggaaatcta tggaacaacg
    13201 tcaaaggatt tgctggagat gttggaaaca agctgaaaga tgtagtcggc gatgtatggg
    13261 attttgtaac agacccggga gcgttggcta ggaaagtgtt aaatggtctt ggcgtactgg
    13321 aagggcttgt caaatatcct ttagatgttg gtaaaggtat tctaagcaag gctaccgaag
    13381 cattgacgaa caaaatcaca gaactattca gcagtggcag tttagacact tcaatgggca
    13441 tgcaaggcgt ttacaaatac ttggcggacg ttgcagttgc agtaatgaag aagtttccag
    13501 gctttgtggc aactagtggg tatagaccag gtgaccccta ttcgcatggt aaacgtaatg
    13561 ccattgatat tgcactacct ggtgtcacag gaggctcgcc acgctacaca gaagcagcca
    13621 attacgcatt tgagaagttt gcaaacaaaa tcggctatgt tatcacaaat ggcaaggttc
    13681 gtgaccgttc aggacaatca ggtacaggta ttcacaatga ttggagacca tggcccgatg
    13741 gagatcacta tgatcatgtg catttaaacg gtgtgaaaga cccacaaaac actcaaattt
    13801 caggagatag cgtgggaggc agtggggtag aaagatggcg caatgtagca attagagcgt
    13861 tgaaaatgac cggtcaatac agtactgcaa acttaaatgc attactaaat caaatgcgta
    13921 cagagtcaaa tggtaatcct aatgcagtta acaattggga tattaacgcc aaaaatggaa
    13981 caccatcaaa agggttgctc caagtgattg acccaacatt cagacagtat gcaatgccag
    14041 gattcaacag caatattttt gacccactat ctaacatctt agcttcaatc agatacgcac
    14101 tatcaagata tggctcacta acaaatgcct atcgtggagt tggttacgca aacggtggaa
    14161 ttgtaaacca acatcaaatt gcggaaatcg cagaaggaaa caagccagaa attattattc
    14221 ctttagataa ggctaaacga tcaagagcga tgcaattatt gtctatcgca atggacaaac
    14281 taggagtttc tcctaaaact tatggaaatg cgacaactgt ttccagtgat tacgaaatcc
    14341 tagaagccat tgaaaaacag gcggcaacaa cgaatcagct gttatcgtta ttgctggcat
    14401 ttttcaaagg aaatagccga actgatagag atttagcttt agatattcag aagatcttgg
    14461 ttaggaggat gtagatgcga actgtattac tgagaaatag aataaacgaa gaaattgatt
    14521 tgtctacgga ggattatttt gctactgggt tgagtaatat ggggtttgaa gtaaaaaaag
    14581 aacatgtggg acagtggggg aatttcagag aaagcagtga gaacgttgaa atatctgaat
    14641 ttcagtcatc tgtaatcatt tctgtgcatg ggtttcgaga aaaagaactg tacaattcac
    14701 ttgtgcagtt cctatcggaa ggtccgtttg aactggaatt tgcttttgac ggcgaaacaa
    14761 tggtaagaag atgcagtcta aaatctttaa gtaagactga aatcgatccg aaaacatcgt
    14821 tactaacaga cactttagaa ctatatttca catcaaactg gtattccgta aaacgcgaaa
    14881 aattaattca gcgaccgaat gtagtgaaaa cacgtggtaa ggtttttccg tataaaagat
    14941 cttatatcta cactcaaaac ttgtgggaga aaaaaggtgt attcaaattc aataataatt
    15001 ctgtatacct aacgaatagc aaggaacgga tgtccccgct aaaaattcgt gtgattggga
    15061 agtgttcaaa tccgtattgg gaagtaatcc aaaactcaca aatcatcgca acggatggat
    15121 acttcataga tatgactgaa acacagactc tagaagtatc aagccttttt gaagatacaa
    15181 cggcgatttt aaaagatatt gcaggggtag aatcttctgt ctaccaacaa caggattata
    15241 caaaaactaa ttttgttcag gccccgactg gagaattcag cattgtgttt catgttgggg
    15301 gggcagacgt agagattgag ttatacgagg agcgtgatct gttttgattt tagcagtcac
    15361 gctttttcat cgtgatttaa tgctttataa cgaacattta ttctctaaca gttttgaatt
    15421 tggtgcggac gagatcaacg aagaggctag tagctttacg atggataagt acgttcctgt
    15481 aaaaacaggc gattttctac tagcaaaata tattcctagt ggaaaatttg catattttgg
    15541 tgtaattacg tcgcaggaag acgaaaaaat cagttgtaaa agcttactta gtttagctga
    15601 tagtgagata ccgactgcac gtgtgtcagg agataactac gaagagcata tccggcggtt
    15661 gattgaatac tatttgctga atgatccaac gaaacaacta aaagatattt tagacgtcaa
    15721 agcagaaagt gcgacctctc attcgtatca agctaccgat acgaataaac acaagttaag
    15781 tgcgtatatt ctcaatggtt tcaaaaaata caacgtaaaa tggtatttca agggaattca
    15841 aaacagaaaa atctatacag gcatacgtgc tgtaaatgaa tcaatttaca ttaaagacaa
    15901 ttcttctgaa tttagcgatt gggatgtgtt tgttcaggcg ccgggcgctg gaaacgagaa
    15961 caagctatta atcgttgata aagcaatgaa agatatagag aaaccgataa tactgtcaac
    16021 atggtatttg gacgaagaaa acaatttgac acaagatgga tcgaaagaaa atatcacgaa
    16081 accaactgtc aatttggtta atatctacga tcaaaccgca gaagataaag catcctacga
    16141 agatgtggca aaatcagagt tgaaaggcaa tacgtattcg catgaaatca aagtgaacgt
    16201 tgtaagaaat gcaaaaaatt tgaatgtcga aacgattgaa acagggatgt ttgccacgat
    16261 ttcttataaa ggaaagatat acaagtcggt tttgacagct tggcgaatat caagtgataa
    16321 ggagtttgtg gaattgactt tcggaaacat aagaagtcgt tttatggatt attttgaaga
    16381 taatggggga taaaaaatgg ttagcaatgt ggatggatat caatttgaaa acgtgaaagt
    16441 aagcgcagaa aatgatgcta gactttatca cgttttatat aatcggaaaa atcaggttat
    16501 tgatggttac gatcagtcta tgaatttatc ttcaagcgga ttaacagtaa aagttgctgc
    16561 aggagcagcg attattcaag gtcgtatggt cgttgttcga caagaagaaa gtataacagt
    16621 tccagcaaac tcaagtggtt atatagcatt aacagtggat ttgacacaag aagttatacc
    16681 tggatctatt cttccagaat cggaagaata tgaatggact aataatcaag tcaagctaga
    16741 atttataaca aaagttataa aaggtaattt aaacaatggt gataaggtct ataatttacc
    16801 actatgctca gttacttcta ctggatcaac tgtttcaatc tcaaagatat cggatagtta
    16861 cgagctgact ctctctaaag gagaaatttt gtggagtggg actgcgttaa tgcatgatac
    16921 tcaaactgtc caaccttcaa aaaaaatgtg gcagacagtt tcaggttttt tattagtatg
    16981 gctcccatac gaaaacgggc aagcaattgg ggatagatat gtaactacgc ctttttataa
    17041 agagcgtgta acttttacta atgttttagg agaaattgtt tcaggatttg atgactatca
    17101 caaaaagtgg tttagtaaac gaataaacta taattcgaat actaatatat ttacaggtgc
    17161 agcaagcaat gcaagtggag ataacgcaaa tatggtgctt aggtatatag tttcttttta
    17221 gttaggaggt atggaagagt ggcaaattta gaaattaaat tatctgcaaa taagagacag
    17281 ccttatctac gtcaccgtgt tgttggtaga gttggtgatg gggggctcac aacaatcaat
    17341 gtacaacttc ttgaggaaga tgaaattaca ccttttgtaa ttaatctaaa cggtactttg
    17401 aaatttgtgg gcgaagtttc aaacggtaac tataccgagg gagaaccaga aataatcgat
    17461 tcgactaatg ggttaattag ttacacgttc actaagtcaa atttcagcac gagtcatcaa
    17521 ttcaaacaag catattttga atatgtagat cctaacggca aaaaagtaac ttttcagaac
    17581 ttcatcatag acgtgttaga acgagtagat attaattcgg agcaagcgaa atactatatt
    17641 tcttcattgg aaaaattaca gagtgaaatg caaaccactt tcaatcagtt cattagtgat
    17701 aaacaggtcc aatacgatca aatctactcg aaatataacg aattagtaag attgataaat
    17761 gaatcagata agcaagtaaa tgatcgtatt gataagacaa atgatcgtat tgatcaaacc
    17821 aatcagcaaa tcggcgatct cggcaagctg aaaaagatgt acagtaacag catcgacttc
    17881 ggggactatg attatagtgg gaatccgaac cttagcgcta aacttaatgc ttcaagtttt
    17941 tctttaggta ctggcgcaac agtcgcggat gataatgatg agattgtgtt taccttagat
    18001 ggcacaaatc aactatcgaa gtacgctagc aaagcgcaag tgccggtagc agaagggaaa
    18061 caatatacga ttagttgtga aattatgtta gaagatgggt tcactggcga cccttcagga
    18121 atacgtctgc aacacgcata tttgccaggc ggggttgttg ttttacagac agagacagta
    18181 cctaaaaatg aattgaatac gtggcagacg cttatcggaa cgcgaacggt aaaatacacc
    18241 tctgacgtac caaatgaatg gtaccctttg tttagagaca tccgaacact taaaccgacc
    18301 ggtaaagtta gactaagaaa aattaagatt gaagaaggct caacagccac cccatatcag
    18361 ccaaacttac ttgatgaccc ttactggcta ggtaaagcgc ctttgggtga gaatattgct
    18421 aataagtctg ttacgtttcc aatcaaatct agcgcctaca gcctatacca agctaacatg
    18481 gaagaagaat ttgttttagg tcaaacctat acaattacca tgaaggcaac caaaccacct
    18541 atccaaacgt tcattgttta caacgaggat agtggagatt atagatatgg taacttagag
    18601 ccagtagaag ggttagttga tacatggggt ctgactttta caccgcagaa ggttggagtt
    18661 aactacccca aacggttaac tattatccag tatccccaat caacaacagg tgcatgtcaa
    18721 attgactggc tcaagatcga aaaaggcaat actcgaaccc cgaatattag tgagtataaa
    18781 tatcgtggta ctggtatgcg tgattcaaac aatccaaaag attatgtttg ggatctagca
    18841 ccagaatatg tcgaagataa tttggccaca gatattaaaa tttctgaaat tactggtaaa
    18901 gcaaacaatt ataccgatgg gaaagtatcg gagattaatt cgcagttgac tgcttcaatt
    18961 aatgaagtag acaccacagc taaggatgct caaacaaaag cgaatgctaa tgcgactgct
    19021 atagatgaat tagacaataa gatcgatgaa cgcattaatg atacagctac taccacatta
    19081 acagttacaa acgggaatac cggatcagca aagctttatc gtgaaggaaa aacagtttct
    19141 atatattttg tggctttaaa cggaaaaagc agtggtggaa atgattcaac gatactaaca
    19201 attccagaag gctatcggcc accaattagt tttgagcaac tggttggctc gatagaccgt
    19261 tctactttga acagtgctca gttatctatt ggtgcagatg gagccattaa atggcgaaga
    19321 aactcaagtt atggatcgga ttataccttt gcaattactt acacgattta gaaaagcgtg
    19381 aatcgatatg aaggcagcat atagaccaat tgaaccttac ggattcgagc aaatcattgt
    19441 gaatgatgaa gaacatttac cggaagaatg cacagaagtc gaaccaccga ttctaaattg
    19501 gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa acatttggaa atatggacgt
    19561 actggcggag agtacgcagg aaaagtattg gacgacatgc ttgtatccgt tccttacaca
    19621 gatcagccac cgcttgaagg aattcgtgct gatggcgaac cgttaacaat cgctgatcaa
    19681 atgtttgatc ctaaattgaa tcaatggatt attttagcga acgcactaga tcacaacgat
    19741 ttaaacaatc tcaaagcaat gtatgagtcg ttagaaaatg agaacggcga tttaaaacag
    19801 atcaatgcca aactcatgct aagcgatgta gcaattaaac aggaaaatac tgcattgaaa
    19861 gaaaaagcgg atagtttagc acaaatcaat tcaaaaatga tgcttgcttc gttacaaaat
    19921 agcaaagaca tttcagaaat taaagagcaa ctaaatccag cttcaaaggg aggtgagtag
    19981 tatgtttagt tttagcgatg tgaaaatgat gtatgattgg ggctgtttta ctgacgatca
    20041 agttcgacta ttcgttccac tatgcattac agacgaagaa gcagataaaa tcattagcaa
    20101 agaagaaagc gcatcttaag tgatgcgttt ttatttaagg taaaggagtt gtcacatgat
    20161 caatttaggg gaatggggaa caatcgcagg atcaattact gcgattgttt ctttgatttt
    20221 attagtaata agaccagtca ctgcatcttt ctcgaagatt actgaaactc tttcaaaagt
    20281 aagtcacaat ttagatttgc tgactaaaga tttagaatcg agcaaatcag atcgattgat
    20341 gattcatgaa gaactaaaga aacacgatga aagattagat acacatgcag aaaaattggt
    20401 agaacacaca caacaaatta aaactttatt tagagaaagg tctcggtaaa aaagaaagga
    20461 gttaagaaga aatgatttta cccgataagt attatcaagt cattaaatgg acagttttaa
    20521 cagtattgcc agctttatct gtattagtag ccacattagg caaagcatat ggatggaatg
    20581 gaacagatat ggcagttctg actatcaatg cagcagcaac atttttaggt gttatcactg
    20641 gtgtgtcggc ttataatttg aaaaaatagg aggaaacaaa tgaaaaagaa aattactatt
    20701 actgcgatga gcctattaac ggctcttttt ttattgccga ttaatgggtt tgcctatacg
    20761 attaacaatg aatttaattt aggtgtaaac gaaggtagtt ctcaagtagc aaataatcag
    20821 tacattttac tgcatgaaac ggctaatgaa acagcaacag gacgcaatga agcgcagtat
    20881 atgcaacgtt catggactag cgcttatact gcttatattg tgggagacgg cggaattgtt
    20941 tatcaagtcg gtcaacctgg ttatgtacag tacggtgctg gttcgtatgc taatgcaaat
    21001 agtcctgtgc agattgagtt acaacacaca catgataaag caacgtttga gaaaaactac
    21061 aaggcatacg ttgaattagc tagagattca gcaatgaaat atggtattcc acttacacta
    21121 gacacaccgt ataatcaacc agggatcaaa tcgcatttat gggtaacaca aaatatttgg
    21181 ggcgatcata cagatcctta cggttatctt tctgaaatgg gcgtaagtaa agaaaaatta
    21241 gcctatgatt tggctcatgg atttaccgat gaaaatccga caacttcaga tgataaacca
    21301 gtcattgatc caactcgagc aggtgctgca aatcctaccc tgacagatgg aacaaattac
    21361 gcccacattg atcagttcgg agaaatcgaa aacgcaaact tacatgtggc tggttggcac
    21421 atcgctaact ataaatacga gtatatcttc attatggatt acaatactgg gaaagaatta
    21481 gctcgagtaa aagctgatgg aatatataga ccagatgtaa atcaagctta taatacttta
    21541 ggaaatgttg gctatcatgt atcttttaac atgcgcaact tccctaataa gaaagtctat
    21601 gtaatgatgc gggcaacgaa tgatccagaa gggaacacta aaggcggagc acaagatttt
    21661 catgataaac gctggtattt aaatattccg caacgataaa aatagcccct cgtttttgag
    21721 gggaagtaca taatgcaaat ttgtccataa cttattgatt atcttgtaaa aatgcggtaa
    21781 tttaatagtt agtgagaaga ggtgcttaat tatagttaaa aaagaggaaa tagaagcagc
    21841 atgtaaccaa gcaaatgtta ggtttattgg agaagcttta tctggatatc ctaagtataa
    21901 atacttacac aactggttac aaagtaaaag tttttatctt aataaagaaa gatgcatgcc
    21961 taataaaaat tatagggttt ataaaagagg aactattgta tatgttgatt ttggggtgaa
    22021 cgtgggttat gagttatctg gtaatcattt tgctattgtt ttaaataata aagataattc
    22081 gaaaaatggt gttgtatgtg tagtaccaat ctcttcaaaa aagaaaagta attatgtatc
    22141 tgtaggaaaa atattagaag ttgctagtat taaacaattt ataagccaag ctgataaatt
    22201 aaaagagaaa cttaggataa ttattttact ttcattaaat agaggcatca ttgatgaaag
    22261 cgatttgaat agttttatta aagatattga agctaaaggt gaaaagcttt cagtggaaga
    22321 agtagaaaaa cgagctgtaa aacataattt ggtttgcgag acaagggaac aagtaattaa
    22381 agaaataaaa tcattatctg aagatatggt aaaatatcag aaggtgttta attgctacaa
    22441 gaaatataca caagaatcct atgtaatgcc acttaatatc caaacgatta gtaaaaatag
    22501 aattagaaga attaacaagt ttgatccttc tggaaatatg aaggcacctg gaaatgtcct
    22561 caatgaagta gatgaaagtt taaaaaaata ttttacaaag taaacgatat ttttgttgac
    22621 atttttctgt tcgttagtta ccatgtagtt atagaaggac gtaagcgcct atttttaatg
    22681 tacacatcgg gacgtatgcg cccacataga aacatagttg agagacatct atatggtgtc
    22741 tcttttttta ttaatatgca aaaaagtcta gttttttcaa taatatttat gtaataataa
    22801 attagccacc acaacaaaga atgaaatcca ttattatcta gtctagtcca ttctttttgt
    22861 ttgcagtatt tgtgatggct ttccgtaccc ttagctcagt tggtcagagc agacggctca
    22921 taaccgtccg gtcgtacttc tcgtaatcca ggaaggccgt cagctccgtc cgcagtagcg
    22981 tgtttacggc cagctccaga tgggagcgga ataactcgtt caaatcacct ttggtggcta
    23041 gagtctgcag gagttctgta ataaaatcgt tcatagggaa gtcctctttt ctgtgaatgg
    23101 g
//
LOCUS       DASJSZ010000044        22973 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.044, whole genome shotgun sequence.
ACCESSION   DASJSZ010000044 DASJSZ010000000
VERSION     DASJSZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.044"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(41..3715)
                     /gene="rpoC"
                     /locus_tag="U4B03_001570"
     CDS             complement(41..3715)
                     /gene="rpoC"
                     /locus_tag="U4B03_001570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEN2068425.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="U4B03_001571"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="U4B03_001571"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN2068426.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="U4B03_001572"
     CDS             8118..9086
                     /locus_tag="U4B03_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2068427.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="U4B03_001573"
     CDS             9104..10009
                     /locus_tag="U4B03_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068428.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="U4B03_001574"
     CDS             10006..10812
                     /locus_tag="U4B03_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2068429.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10948..11937
                     /locus_tag="U4B03_001575"
     CDS             10948..11937
                     /locus_tag="U4B03_001575"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEN2068430.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="U4B03_001576"
     CDS             complement(12254..12778)
                     /locus_tag="U4B03_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEN2068431.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..14876
                     /gene="scm"
                     /locus_tag="U4B03_001577"
     CDS             13065..14876
                     /gene="scm"
                     /locus_tag="U4B03_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN2068432.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15031..16017)
                     /locus_tag="U4B03_001578"
     CDS             complement(15031..16017)
                     /locus_tag="U4B03_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN2068433.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16040..16606)
                     /locus_tag="U4B03_001579"
     CDS             complement(16040..16606)
                     /locus_tag="U4B03_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEN2068434.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16620..17036)
                     /locus_tag="U4B03_001580"
     CDS             complement(16620..17036)
                     /locus_tag="U4B03_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN2068435.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17493..19427)
                     /locus_tag="U4B03_001581"
     CDS             complement(17493..19427)
                     /locus_tag="U4B03_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2068436.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19708..20664
                     /locus_tag="U4B03_001582"
     CDS             19708..20664
                     /locus_tag="U4B03_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEN2068437.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20754..21857
                     /locus_tag="U4B03_001583"
     CDS             20754..21857
                     /locus_tag="U4B03_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN2068438.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21870..22823
                     /gene="menA"
                     /locus_tag="U4B03_001584"
     CDS             21870..22823
                     /gene="menA"
                     /locus_tag="U4B03_001584"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN2068439.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT         6746 a         4864 c         4496 g         6867 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    14161 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    14221 caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa
    14281 gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct accacaagtg
    14341 atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata
    14401 gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca agtgatagca
    14461 cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt gatagctcag
    14521 attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac agtacagata
    14581 catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt acagacacgt
    14641 cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca agttcggata
    14701 acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac aaatcaaaaa
    14761 gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca ggcgttatat
    14821 tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa aggtaaaaaa
    14881 atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt tttgcttttt
    14941 atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt ccttctgaga
    15001 aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa tcagttgggt
    15061 aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct ttatttcctt
    15121 caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc ctcctgtatg
    15181 ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt tcttctctaa
    15241 ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta aagaatagac
    15301 cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt ttgtataatc
    15361 taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat ctcctgcttt
    15421 ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac ttaattggaa
    15481 cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa gttcataatt
    15541 atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac tgcgagaatt
    15601 caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac agcagacaaa
    15661 taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga tattaggctg
    15721 accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg ccgtatggag
    15781 catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg gtccaaattg
    15841 tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga tcgtttcttt
    15901 gattgcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc gttttagtgt
    15961 ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta gcttcataaa
    16021 aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt tgtttggttc
    16081 cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa agataattcg
    16141 ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact ggcaagtaca
    16201 acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg acattatgca
    16261 tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct aattgcttga
    16321 ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg aatgtggaaa
    16381 tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag aagctgtctc
    16441 gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct agttttgcac
    16501 caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg atcactcctt
    16561 gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg tgtgaaggtc
    16621 taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat cactaattta
    16681 tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg tacgcagatc
    16741 tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc gatcaagtta
    16801 taatctccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc catcaaaggg
    16861 aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc tgttgcggtg
    16921 ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa aagcaaaata
    16981 ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag gctcattttt
    17041 gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag gttgtgacac
    17101 ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca tgttttctaa
    17161 ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca agaggttgtg
    17221 acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt ttcaagtttt
    17281 tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt ataacaagag
    17341 gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc gggcatctta
    17401 ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc tttctattga
    17461 aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg ggaacttggt
    17521 ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca gcgatccaac
    17581 ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa ccaaatgttt
    17641 acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt tgtcgcactg
    17701 catagccatg tgaataatcc agcaatcaat gctaatgcca aggcaatttc aaagaatacc
    17761 atgaattttt ggaagaacat tgctacatca ttgtttggca tgatcacttg catgatcttt
    17821 gtgaaccagt ctggtgactt ctcaatcaca gccattggtt cttcaccgta tgcataactc
    17881 aaaccaaaat gtgcagcttg agtcgctgct tctccggcac ttgcccctgc tccgctggca
    17941 gctgtggttg catcaccagc agcagaagca ccactggcaa catctgttac attgtattcc
    18001 catgtccaag ggaacacact gtttccagtg aaccatgatc cttcaccaaa ccaagtactt
    18061 ggtttgaaca cttcaccatt cccaatgatt tttttggaag cttcaacgaa ccaaactaaa
    18121 ccatagaaaa tacgaagtgg cacactccat aagacattgc cgtaacgaga agaatgtcct
    18181 cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat gcattaaata ttggaacata
    18241 taataaccag aacgaatatc aaagaagtat ttcaaattca cgatatgttt cattacgata
    18301 gctaaaaatc cgcttaaatg gatcttatca aataggttag ctacccccca tttggcacca
    18361 atagaaacca taaatccttg atagtttcct ttaaatttat gcttttctcc gcctttgatc
    18421 gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg aacgatctgc
    18481 ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat caccaatgat atagatgttt
    18541 ttatcttcgt agccttttgc ctgcatatat tcattggcga tcaaccgctg tccgcgtgct
    18601 gattctaatc caaagtcagc agcgtcagaa gttcctttga caccagctgt ccaaatcagt
    18661 gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat gatcagcagc cacttctacg
    18721 attggtgcat tcaataataa ttccacgttg tgcttacgca aatagcgttc agcttttgca
    18781 gcatcatttc gatccaacat attcaagatc gtcggcattg cctctacgac catcagtgtg
    18841 atttcgtttg gatctaattt ataatctttg gctaggcgat ttttccagtc gatcagttcc
    18901 ccaatcattt caataccggt aaatccagaa ccacagacaa cgaaagtcag catggcttta
    18961 cgcagttcat catctggttc caatgcagct tttgctactg tcttctcaat atgttcccga
    19021 atccttacag aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac accaggtgta
    19081 ccaaagtcat tcggctctcc gcccattcca aggatcagct gatcaaaagg ataggaacct
    19141 aatttggtct tgacgatttt tcgttcttta tcgataccga ttacagtgtc tgtgacaagt
    19201 tgtacattct tttgacgagc aaacaaatgc tgcagatcgt attggattgc ttctggttct
    19261 acacgtccgc cagctacttc atgcaactcg gtcatcatag tatggtacga atgacgatcg
    19321 atcagagtaa ttgctacatc ttcttttttc agtttctttg ccaagtattt tgtagctgaa
    19381 acaccggcat atcctgctcc tacaacgaca atattttgct ttgccattga tcatccgctc
    19441 cttatagact atctatataa tgaatatatg ataattcttt tagtttgata cttattacac
    19501 aatctccata tattatatag atgttctgtt cttttgtcta actccttttg gatttttttc
    19561 aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa agaaaacgtt tgaaatatta
    19621 catgggttat ttatcaaaaa tagttgactt ataggctaaa aagattagaa tgtataatga
    19681 atcacaaaga aaaattgtgg actagaaatg gaatggtggg gtattaaaat gaagaaaaaa
    19741 cgttttgtaa ctggatttgc cgtattagca ttttcagctc tagtgttagg cgcttgcggc
    19801 gcagatgaca acggaagctc aaactcttct tctgaatcat ctacagcaca atcttcgact
    19861 gcaaaaagca caacagaatc atctgcaaaa gttgtagctg gcggagattt gcaagacggc
    19921 acatataaat tagaagaaaa aaattattca aatggttacc gtgcagtctt tgaaatggtt
    19981 gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg tcaatgaaaa cggcgaatca
    20041 aaaacaaaag atgcagaata caacaaaaac atggaagcaa aatctggcac aaacccagaa
    20101 aaatacattc ctgagttgaa tgaccaattc ttaaaagcac aaagcgcaag cggtgtggaa
    20161 gtcgtaactg gagctactca ctcatctgaa agcttccaaa actacgcaca acaattgatc
    20221 caagctgctc aagctggtaa cacagataca atcgaaatcg acaatggtgc tgatttgaaa
    20281 gacggcactt acaagcttga agaaaagaac tactctaacg gctaccgtgt gcaattcgaa
    20341 atgacagttg ctggcggcaa agtaactgaa tcaaactttg attatatcga caaagacggt
    20401 aaatcaaaac aagatgatac agaatataat gaaaatatga aagcaaaaag cggtacagaa
    20461 ccaaaaactt acatccctac tctgaacgac gaattcgtca aagcaatggg cgaagaagat
    20521 ggctcacctg ctaatgtaga agttgttaca ggtgctacac acagctcaca ttcattcatc
    20581 atgtacgcac aacaattagt gaatgcagct gaaaaaggcg acactcaaac aatcgaagta
    20641 gataatatcg taacagaaaa ataatcacat aatcatacta ataaaagttg gagggtggga
    20701 cgatagccag attaggcagt cgtctcacct ttcaaaatga agagaggaaa aagatgcgga
    20761 aaaaactact attttcctta atcacactgg gcatctcttt tatggtgcta actggctgca
    20821 gcaactcagc aaataaaaca accactgaaa gttcagcagc taccaagata ttgaaagatc
    20881 catattctga tgaacagttc ttgttaggga cttacgttcg tatccgtgtg tatgatgaag
    20941 gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa agaacttggt gataaaatca
    21001 cgatcaatca aaaaggttct gagatcgatg aagtgaatga gcaagctggg atcaaaccag
    21061 taaaagtttc tgatgacgtc tacacgttag tcaagcgggc ctacgaatat agccaagatt
    21121 ctcaaggcgg attcgatatg gcgattggtg caatcacaca attatggcga atcggttttg
    21181 atgacgccag aaaaccttcc caagaagaaa tcgatcaggc attgaaatta gtggattatc
    21241 ataagatcga actaaatgac aaagaaaaaa ctgtttatct aaaagaaaaa ggaatgatca
    21301 tcgatttagg tgcaatcgcc aaaggttaca ttacggacga agtagtgaaa gtcttgaaaa
    21361 aacaaggagt gaccactgcc atcgttgact tgggcggtaa tgtctatgtc ttaggccaca
    21421 gcccaagagg cgaaaaccaa gactggacag tcggcatcca agatccgaac atggctcgcg
    21481 gatctgtttt aggttctatt aaagaacgga acaaaacatt agttacctct ggaatttacg
    21541 agcgttattt agaagtagat ggcaaaaaat accatcattt gtttgatcca aaaacgggtt
    21601 atccgttcga caatgatatc gcaagtgtca cgatcataac agataaatca attgatggtg
    21661 acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa aggactggaa tacgtcgaaa
    21721 gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa agacgataaa gtatatgtga
    21781 ccgatccaat caaagacacc ttcaaattat cggaggactc ccattacacg atgggtgacc
    21841 gttctgaact aaaataagga gtacgaacca tgacactaaa aatcttttta gaagtagttg
    21901 aaatacgaac aaaggtggct agtgtatttc cttttgcggt aggggtatta ttctctatcg
    21961 ctttttttca tgagatacat tggctaaaca cttttttatt ttttattgga atgctagttt
    22021 ttgatttagc gactaccgct atcaataatt acatggatta tgaaaaggct cattctgaag
    22081 tatataagta tgaagaaaat gtcattggcc gcagaaatct atctcctgta ttgattcgaa
    22141 acatgatttt agctatgatt gcatttgttt ttgttatcgg cgttattcta acgatcaata
    22201 cgggttggct tttacttttg atgggattta tctgttgttt tattggagta ttctatacat
    22261 tcggaccgat tcctttatct cgaatgcctt taggggaaat tttcagtggg gtgactatgg
    22321 gccttggaat ttttgccatg gtcgtttatg tcaatacatt cggttcagat gtctatgatc
    22381 tgacagtcaa cttttcatct ggacaatttg ctttggtagg aaatctttgg gctatagcag
    22441 caatcgtatt agcttcctta cccttggtgt ttacgattgc caatatcatg ttggctaata
    22501 atttgcggga tcttgaccgt gatattgaaa atcatcgcta tacactagtt ttttatatcg
    22561 gacgtcctgc gggaacagtc ttattccagc tattgatgta tgcttgctat ttcgtgattt
    22621 taatggggtt gttgagcggt atctatgaat ggccgatctt ggtcaccttc ttgaccttgc
    22681 caattgtgaa gaagcatttg gatcaattca agcaaacgtt accccagcct atcagtttca
    22741 gctattccat caaaaatatg gtcttattca atgccagtta tgtattgggg ctattactaa
    22801 caatcattat gcaagccatt taacagcatg aagaggttgt gaaaaaactg ccctgcatca
    22861 agtaataaga acagccaaat aaaaatagct ccttatattt ttcgtttggt tgggcttatt
    22921 accgcagatg caagcttttg aacaccgttt atcaggaggt tgtgaaaaag tcg
//
LOCUS       DASJSZ010000045        22763 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.045, whole genome shotgun sequence.
ACCESSION   DASJSZ010000045 DASJSZ010000000
VERSION     DASJSZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.045"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            48..458
                     /locus_tag="U4B03_001585"
     CDS             48..458
                     /locus_tag="U4B03_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEN2068440.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            451..1152
                     /locus_tag="U4B03_001586"
     CDS             451..1152
                     /locus_tag="U4B03_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEN2068441.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1152..2738
                     /locus_tag="U4B03_001587"
     CDS             1152..2738
                     /locus_tag="U4B03_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2068442.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2757..2978
                     /locus_tag="U4B03_001588"
     CDS             2757..2978
                     /locus_tag="U4B03_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEN2068443.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2975..3736
                     /locus_tag="U4B03_001589"
     CDS             2975..3736
                     /locus_tag="U4B03_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEN2068444.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYVLGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4116..4625
                     /locus_tag="U4B03_001590"
     CDS             4116..4625
                     /locus_tag="U4B03_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN2068445.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4700..5239)
                     /locus_tag="U4B03_001591"
     CDS             complement(4700..5239)
                     /locus_tag="U4B03_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEN2068446.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5379..5990)
                     /gene="rpsD"
                     /locus_tag="U4B03_001592"
     CDS             complement(5379..5990)
                     /gene="rpsD"
                     /locus_tag="U4B03_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEN2068447.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6252..7103
                     /locus_tag="U4B03_001593"
     CDS             6252..7103
                     /locus_tag="U4B03_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEN2068448.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            7131..7766
                     /locus_tag="U4B03_001594"
     CDS             7131..7766
                     /locus_tag="U4B03_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEN2068449.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7786..8559
                     /locus_tag="U4B03_001595"
     CDS             7786..8559
                     /locus_tag="U4B03_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2068450.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8967..9764
                     /locus_tag="U4B03_001596"
     CDS             8967..9764
                     /locus_tag="U4B03_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEN2068451.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9742..10155
                     /locus_tag="U4B03_001597"
     CDS             9742..10155
                     /locus_tag="U4B03_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2068452.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10139..12784
                     /gene="mutS"
                     /locus_tag="U4B03_001598"
     CDS             10139..12784
                     /gene="mutS"
                     /locus_tag="U4B03_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN2068453.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12802..14910
                     /gene="mutL"
                     /locus_tag="U4B03_001599"
     CDS             12802..14910
                     /gene="mutL"
                     /locus_tag="U4B03_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEN2068454.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14932..15492
                     /gene="maf"
                     /locus_tag="U4B03_001600"
     CDS             14932..15492
                     /gene="maf"
                     /locus_tag="U4B03_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEN2068455.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15577..17565)
                     /gene="treP"
                     /locus_tag="U4B03_001601"
     CDS             complement(15577..17565)
                     /gene="treP"
                     /locus_tag="U4B03_001601"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEN2068456.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17788..18501
                     /gene="treR"
                     /locus_tag="U4B03_001602"
     CDS             17788..18501
                     /gene="treR"
                     /locus_tag="U4B03_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEN2068457.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18578..19024
                     /gene="msrB"
                     /locus_tag="U4B03_001603"
     CDS             18578..19024
                     /gene="msrB"
                     /locus_tag="U4B03_001603"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN2068458.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            19194..19796
                     /gene="ruvA"
                     /locus_tag="U4B03_001604"
     CDS             19194..19796
                     /gene="ruvA"
                     /locus_tag="U4B03_001604"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEN2068459.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19809..20810
                     /gene="ruvB"
                     /locus_tag="U4B03_001605"
     CDS             19809..20810
                     /gene="ruvB"
                     /locus_tag="U4B03_001605"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEN2068460.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20839..21417
                     /locus_tag="U4B03_001606"
     CDS             20839..21417
                     /locus_tag="U4B03_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2068461.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21469..21951
                     /locus_tag="U4B03_001607"
     CDS             21469..21951
                     /locus_tag="U4B03_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEN2068462.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22068..22442
                     /locus_tag="U4B03_001608"
     CDS             22068..22442
                     /locus_tag="U4B03_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068463.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7404 a         4069 c         4926 g         6364 t
ORIGIN      
        1 aaaggtttgt tagtaaaatg aaagctatac gtaaggagtg ttgtttgatg aaattttttc
       61 aagagttttt cattattatt ttcttttctg tattaggaga aggcatacgg atttttgctc
      121 atttacctat tcctggaagt atcttaggta ttcttttttt atttttagca tttgaaatgc
      181 atcttgttga tcctgaaaaa atcggagcaa ctggtgattt tttattgaat aatctagcca
      241 ttttatttgt tcctgctggt gtaggtttat tggaatattt tgatgatatt gcttcgatat
      301 ggcctgtttt actgggagca gtcgtcgtgt gttccatcgt cacgatggtt gcagcgggaa
      361 aaacggcaga aggtgtagaa gctttgctgg gctatgtccg taagaaaaaa gcagaaaggg
      421 tatctgtaaa cgaaatggag gagaatcaaa ttgactgagt tttttgtgaa tccattattt
      481 ggcttaagtt tgactttagg agtgtacgta ttatctgaca aatttttgcg gaaatataaa
      541 ctgcctgttt tgaatcctct cgtattatcg attgttgtta ttatcttatt tttgcatttt
      601 tctgggatca gctataaaga ctataatatc gggggcaatt ttttgactat gctgattact
      661 cctgcgacag tggcacttgc catccctctt tataaaaact tccgtttact gaaagagaat
      721 ttttatcctg taatggctgc gattttagca ggaatagtcg caaattgctt ggttagtgta
      781 gggttcggtt acttcttctc tcttcataaa gagatggtga tctcattact acctaaatct
      841 gtaacgacgg ctatttctgt cgatttatct catacgatgg gcggggtgaa tgccgtgaca
      901 cttgctattg ttgtctctac aggtattttt ggttcgctga tcgccactca tattttccgc
      961 ctattcaaga tcgaaagtcc agttgctcgc ggtgtggcgt taggaagtac ttctcatgct
     1021 attggtacag caaaagcaat cgagattggt gaaatagaag gaatcatttc cggacttgcg
     1081 atatgcgtca acggaatatt gaccgtttta ttgctaccgt tattctttca gccttttgct
     1141 ggcttgtttt gatgcgaaaa ccactatttt atgctacaat aaccaaaacg ttcgagcata
     1201 aaaaaggagg gaaagacgtg tatttgtctt ccgagtatct taaacaattt gataaagatc
     1261 tatattataa gattctcctc ctagaaagtt ttgaggatca agcttggcat accgctgcac
     1321 aattagcgca agttgttcag ctggatgccc gcagcgtgtc gaaatacctg aacgagctgt
     1381 ctaaaaacta tcagcagttt agcggtaaga cacacccttt gtttacaaaa aatcaccgtt
     1441 ctggttataa cttttatgat acgctcgatt caattgaaca tgagcgtttt ttgatctatc
     1501 tcgtgcaaag tacgctgaaa ttccagttgc tgcacgacat tttttttgaa gaattccata
     1561 caatgtacca atttgcgcaa aagcattaca ttagtgaatc tactgctcat cgaaaaatca
     1621 atgaatggaa acagcaactg caaacttacg gaattcggct gcaacgaggt acatatatcg
     1681 ctcaaggcga agaagaaatc attcgacttt acttgcatat gacattctgg cagttgtttc
     1741 gagggaaaat ctggccattt gaaacgattt ctcaaatgga cgtaaagaat atggcagaac
     1801 atatcatggc tttttttaat gttcgtctga acgagatcaa aaaaagacga ttggaatata
     1861 tgctaggtgc ctttttttta agaaaatcac agaagcatta tgttgtattg aatgagaaaa
     1921 aaagacggct gatttctgac aatctgttgt tccagcgatt ttgccaggtc atggagccgg
     1981 tatttcctaa ttattttcaa gtagaagacg agttaggcgc attatttctt gtactgatga
     2041 ctcgtgaaga atattatagt gatcctaaga tacggaagaa aatttttgat tttcatcaac
     2101 aagcaaaaac accgcccttt acagcccttt cagaagcaaa agccgctctt tctctttatc
     2161 aggaagagca ggggctgcca gcagaaaatc taaccttcga ggcagaaaac tatttattca
     2221 gcagtcattt tttcgcctat ctttttccta atgcgaaaga aacgattgat ggcaatagta
     2281 gtgattttat taatcattta gttattgaaa acaaggaatt gaaacaatgg ctcgtacatt
     2341 tttttgaaag tagacacaaa catcccaatc atctggcatt taaaaatcat gcgtttttga
     2401 tggggcggta tctgactgtg tttaaaacat taggagcctt tactccacag cttccaaaga
     2461 tcacgatcct tttaatgaca gatttcccat tatttgaaga gcaactgtta gaggagggat
     2521 tacggacatt tttccgtaat gagtatcagc tgatttttct gccaacagat tatcggggga
     2581 gagaagttga tcttctgatt tcgacaagta aagtccaccg caaaccatgg gcggatcttg
     2641 actattttat tgtgactgaa gaactaaaat taattgatta catccaacta tctcaaaaat
     2701 ttgaaatgat tcaaaaacaa aaactattaa aacaataaca aagaaggaga aaaaagatga
     2761 catttgaaga aattttacca gaattgaaaa aaggtgcaaa gattattcgt aaaggatgga
     2821 gtggatttga actttatgtt gttctcgtat ccgatgatga atttgatggc tgtccggtga
     2881 cgccgtactt attgatcaaa acaagtgacg aaggtttttc cagcttcgct cctacagttt
     2941 gtgatatttt agcagatgat tgggcgatcg tagaatgaaa aaccagtatc cagatctagc
     3001 taaacaggtt gtatttatca caggagctgc ttctggaatt ggtgctgcgc aagcccgagc
     3061 gtttcttgaa caagatgctt ttgtatttgg attagatcaa aagttcggta aaatggaaga
     3121 aatttctttg cgatttcctg aaacttttgc ctacgtacta ggtgatgttc gacaaatgaa
     3181 agaactccaa caagcggtat cgaaatgcca aaatcatttt ggtgaagtta cgattttact
     3241 gaatacagcg gggatattgg atgcctacaa acccttgatg gaaactgacg aatcgctgtg
     3301 ggatctgatt tatgagacga atgtgaaaag catgtatcag ctgacaaaaa tcatccttcc
     3361 tgatatgatc cgccaaaaaa gcggaacgat catcaatatg gcttcgattg ctggcttgat
     3421 tgctggtgga ggtgggatcg cttatacaag cgcgaaacat gcgattgtcg gatttaccaa
     3481 acaactcgct ctggattacg caggacaggg tatccgagta aaaggaattg ctccaggagc
     3541 catccaaaca ccaatgaatg cggctgattt tgaagggaaa ggtgagatgg cagagtgggt
     3601 ggcaaatgaa acaccagtca agcgatgggc acagccggaa gaagtagctg agctgacgtt
     3661 atttcttgcc tctcctcaag caagctacat acaaggaacg atcatcccaa tcgacggcgg
     3721 atggttgtta aaataacctt gtgttttttt caagcttctg tatgataaaa ctaaagagag
     3781 aaacaacggt tgatgataaa aaaagtcgat gactgttccc gacattcgat gccgaacagc
     3841 cagttcaaaa aattcttttt aataatcgtt aatattcgaa actaagcggc ttttcacgac
     3901 ctcttgatat aaacgatatt caaaagcttg caactgcggt aacaagtcca gccaaatgaa
     3961 aaatataagg agctattttt atttgaccat tcttattact tgttgcaggg cagctttttc
     4021 actaccttct gcgacacttc agttggaatt ttcttcccat cctttggtcg cggtcattac
     4081 caagataaaa ttatcttgca aaggagaaat agaacatgga aaaaaataat acccgttcag
     4141 ccaaacgctg gacaacgaca gatgtgactc ggatggcgct agtcactggt ttatatgtgg
     4201 cagtgacgct ggttttgtct gtctttagct ttggagtgat tcagattcgt ctctcggaga
     4261 tgttcaacta tctgtccttg tataacaaaa gatacatcgt cgcggtaaca gcgggagtag
     4321 ctttggctaa tattgcttct cctcttggac tgatagatgt tgcagtagga agcgtcagca
     4381 cgctgcttgt attgcttatc aactataaaa tcactagtcg catcaaaaac atgaaaatca
     4441 aaatggccgt gacagcatgc gtatttgctt tttccatgtt tacagtagct ggtcagctaa
     4501 cgattttata tcaactacct tttttcttga attggtgggt gattgcttta ggagaattgg
     4561 catcaatgat tgtcggtggg ataattattt attggatcgg taaaaaagtg gatttgtcaa
     4621 aataaagatc tgtttaattt aaaatagggc agcatggaag agactgaatg ttgagtcttc
     4681 ttccatgctg cttttttgat taatttgtat gaagaaatcg atttagccgt tttttcatca
     4741 agataccgat ataagctgct ccagttgcct tgattgcttc cggcagtaaa aacggcagta
     4801 gtccactggc aaatgcagtt gtaaaagtaa gatcaccact tattttcagc cagatcgttc
     4861 cgcagatcaa tgcagcaaga gagcccatta gattggcaaa gattgcatat aagtaatgag
     4921 catccgttct ttcgatcatc caaccagtta tccacgtttg taagagaaat ccaatcaaat
     4981 atccgccagt tggcccaaat aaaacagcta tacctccagt catccctgaa aatacaggaa
     5041 gaccaatcaa gccaagtaaa agataaactc caacagatat caatcctagt tttcttccga
     5101 gaaaagttgc tgttaatcca atagcaaatg tttgccctgt caaaggaacc aaaccaaaag
     5161 gaatggtcat ctgagaaagg acagaaataa tcgcggcaaa ttccgcagca agtatcaatt
     5221 ctttcaatgt tcggttcata acgccctctt ttcgttaact taatattttt atttggttaa
     5281 cgaaattgta aaacaaagtg attactttgt aaatagaaaa tataaaaaaa gaccagctat
     5341 ccaatagata gcggtctttt tcaagaaagc ttttgaaatt ataagctttg gttgtagtat
     5401 tcaacgacaa gagcttcgtc gatttctgga tatagttcgt cacgttctgg taaacgagtc
     5461 aatgaacctt ccaatttttc agcgtcaaag cttacgaatg ctgggcgtcc aacagttgct
     5521 tctacagctt cttttactgt tacgatgttt tgtgattttt cacgaactga gatcacttga
     5581 cccacttcta cgtggtatga agggatatca acgcgtttgc catcaactaa aatgtgacca
     5641 tggttaacta attgacgtgc ttgacgacga gtcgttgcaa gtcctaaacg gtaaacaaca
     5701 ttgtctaaac gttgttctag taagatcatg aagttaacac catgtttacc ttctttgatt
     5761 ttgctagctt ttacgaacaa gttacggaat tgacgttcgt tcattccgta catgtgacga
     5821 agtttttgtt tttcagttaa ttgcatgccg tattctgatt gtttgccacg gctattaggt
     5881 ccatgttgac ctggtgcgta agggcgacgt gctaattctt ttcctgtgcc tgacaatgag
     5941 atgcctaagc gacgagaaat tttccatgat ggtcctgtat aacgagacat taaaatttcc
     6001 tccaatgaat ataagttttt ggagtaaaat aatccgttga aaaattcata atcatatagt
     6061 ttgttcttca atcttcacct ttgcagccgc ggttacacaa tcgaacccga gacggggcaa
     6121 caaactaggg atgagttatt atttttctgc tgcattattt tacacaaatt acagtatatg
     6181 atagatcatc cacgaatgtc aagataatta gtacataaaa acgctttaca aacctaggct
     6241 gtctttataa aatgagaggg tatgtgcaaa tggaaaaaag cgaaggaagt ttaaggagaa
     6301 aatcaatgaa tccggtatca ccattatttg agaaaatcga tgcttcaatt gaaaagaagg
     6361 cgaatctttt tgaaaacagc ttttcacgtt acgctgttcg agcggttctc gcctgtttgt
     6421 ttttgacttt aggaacggct gtggcatttg cgatcgcgat gaagggggaa gatatcgcgc
     6481 atggtctagg aaagatttta tacgcattta tgttcagctg gggtctggtc atgattctat
     6541 acatgaacgc cgaattagga acatcgaata tgctttatat gaccgtgggt gtttttcggc
     6601 agagacttgc tttaagtaag gcagccaaga ttctatttgc ctgtattttt ttcaatatga
     6661 ttggcggtat attatgtgga tttctaattt ctatgactac gccatttcaa gatttaacgt
     6721 tggacaactt tatgttagaa tcaatagcag taaaactagc aaaaccttca gcaacgattt
     6781 tggtggaagg gatgtttgcg aatatcgttg tcaataccgc tgtactgatc agcatgcgga
     6841 tgaaagagga tgtcggaaaa gtgatcacag tcgtattcat cattttcatt tttgcttttt
     6901 taggatatga acacgtgata gcgaattttc ctgcctttac gcttacttac tttgcttctc
     6961 acggacagat ggatggaatg acagtcagca atgtgttaca taatttgttt tttgctttgg
     7021 caggaaatta tattggtggc ggtttagtaa tgggactggg atatgcttgg ctgaatcagt
     7081 caaaatcatc ctatgtggac taaaaagtcg ttcattaaag gggaagtgcc atggaagttt
     7141 tagtttttcc aaaaaatgag gaaaaagcgt tacaaatgga aaagtacatg aaaaatcttt
     7201 tttcctttgc tggagtacct aagccggaac gcgctctttt acaacaaccg ttactggatg
     7261 agagcaaaaa tttatctatt tctgaagttt tagcttttgt tcattattat tatgaacaaa
     7321 tcgaaagaga gtatcagtat gtagcgatcg atttagtcca gaaaaacatc aaacggctta
     7381 catttgaaga cttagtcgta ttgttgccac tagttcagaa aaaagaatgg tgggacagca
     7441 tcgactcttg gagaaaagta tacagcacat ggtgtaaagc ctacccagag cagcgtgaag
     7501 aagtctttca attgtttgaa aaaaaggaag atttttggct acgtcgaata gcaatcacgt
     7561 tgcaattagg ttataaacag caaacagaca caggactatt gatccgagca atagacgaag
     7621 atcgtcaaac aaaggaattc tttatccaaa aagcaattgg ttgggcatta cgcgattata
     7681 gtaagaccga tgccgattgg gtaaaaagac agctgacaac agatttgagt aagttagctg
     7741 ttcgagaggc aagtaagtat ttataagaaa gcgagacgac aagagatgag acgcaaatta
     7801 tttgcttttg atattgatgg aacgttgtta gattcaaaca aacaagcttt agatagcaca
     7861 agagaagcat tggaaaaatt acgcaaagcc ggtcatctgg tcacgattgc tacagggcgg
     7921 agccgatttc atgcgcaaga gatcattcgc gatcttgatt ttacgaacta cattttatgt
     7981 aatggagcgg ccggtttttt agaccatgaa caagtatata agaatttact agatcgcgac
     8041 cagttggatg cgttcgttca agaagcgcat catgagcaga tcgatacagc ttttgtcagc
     8101 ttagacaata tcaagcgttc ttcttccaat gatgtaacgg tgatggaaga agcgatgcat
     8161 tctttcggtg ctgtcttgcc tgaattagat cagttttttg cagaggaaca agaaatatac
     8221 caagcgttag catttttcga tcaaacatat gatgggaagt tctcttcata tgataaaatt
     8281 cgatttgtcc gctggcatga gaacagtgta gatgtcgtac ctaaaaatgg ttctaaagca
     8341 gcgacaattc tgcacttagc agaacaagta ggcatccctc atgaagacat catcagcttt
     8401 ggcgatggac aaaacgaccg tgaaatgttg cgcatgtcag gaattgggat agctatgggg
     8461 aatgctgtgc cagaagtaca agcagaagct aagatggtca ctgatacaaa cgatcaggat
     8521 ggaatttgga aggcattgaa agagttaagc gtgatttaaa agaggttgtg aaaaagctgt
     8581 tctgcatcaa gtaataagcc cagccaaaca aaaatagctc ctcctatttt tcgtttttct
     8641 gggcttatta ccgcagatgc aagctttcga acaccgttta aggaagaggt cgtgacagaa
     8701 gtggtcagtt tcaagaaata agaagggatt tacgaaaatt gcttctcaaa tttttgtaaa
     8761 attacggctt atttctgaag aagtagatga aacaacgttt attcgggaaa tgggactgtg
     8821 acaagatgtt tgtcatagtc ccattttttt attttatttt tcaaaactaa gaaataccag
     8881 gaaagagttc cttttgtttg accttttctt tcttattgaa aacaactata atatagagtg
     8941 atgttaaaag agaagaggaa tttagtgtgc gggtattgtt tattggtgat gtagtaggtt
     9001 caatgggacg agagatgatt actgaatatc ttcctagatt gaagaagaag taccgtccgc
     9061 aagtaacgat tgtaaatggt gagaatgccg cttctggacg tggaatcaca gaaaaaatct
     9121 ataaaaaatt tttgcaagac ggcgtagatg tcgtgacgat ggggaatcat acgtgggaca
     9181 accgcggaat ctttgagttt gtaaatgaag ctaaaaagat ggttcgtccg gcaaattttc
     9241 cggaagggac gcctggacaa gggatggtct ttgtcaaagt gaaccaagtc gaactggcag
     9301 tgatcaacat gcaggctcgt tcttttatgg tggacttgga tgatcctttt cgtaaaatga
     9361 atgagctggt agaagaagcc agaaaacgta cgccgatcat ttttgtagat tttcatggag
     9421 aaacaacaag tgaaaaacaa gcaatgggct ggtttttaga tgggaaagtt tcagcagtgg
     9481 tcggtacaca tacacacgtt cagacaaatg atgcgcgaat tttgccaaga ggaacagcgt
     9541 atctgacaga cgttgggatg acaggtccct atgatggtat tttgggtatg cgcagagaac
     9601 ctgtaatcga aaagtttctg actgcattac caaaacgttt tgaagttgtg gaacaaggcc
     9661 gatcgatttt aagcggctgt atcttggaat tggatgatac gacaggtcat gcaaaagaaa
     9721 tccagctgat tcagatcaac gatgatcgac cgttcatgga gtagaaagag aggcagtttt
     9781 ttgcagcagg agccggaaat tcaaaaagaa gtaaggaaac tgacaaaact tttaagagag
     9841 aatgaaacga tcattcgcta caaagaactt gaagaaaaga tccagcaaaa ccagtattta
     9901 gctgagttga gagaaaaaat caaacaagcg caaaaagatg ctgttcattt tgcccactat
     9961 gacaaaccag cagcagaaaa agaagcaatc aaacaagcgg atcaattcat gcaagaattt
    10021 gatcagcatc cgcttgttgt agcttataga aaacagctgt tggaagcaga tgatctgctt
    10081 catcatttaa caactatgat acaagaagaa atcaatggac aaatagagga ggaaaaacat
    10141 gcctcaaaaa actaaaaata ctcctatgat ggaacaatat ctaagcatca aggcccaata
    10201 ccaagatgct tttttgttct atcgtttagg agacttttat gaactatttt acgaagatgc
    10261 gatcaaagcg tcacagcttt tggaattaac gttgacgagc agaaatcgaa atgcagaaga
    10321 acctattcct atgtgcggtg tacctcacca tgcagcacaa ggatatatcg atgccttgat
    10381 tgaaaaaggc tataaagtag cgatttgcga gcaggtagaa gatccaaaga cgactaaagg
    10441 gatggtcaag cgcgaagtga tccagctagt cactcctggt acagtcatgg acagcaaagg
    10501 attgtcggca aaagacaaca attttttaac agctgtagtt agtcaaggaa atgaatttgg
    10561 ctttgcttat gccgatttaa gtacgggaga attgaagacg gctcggttgc atgatgaaga
    10621 agctgtttta aatgaagcct ctgcattgca gacaaaagaa ctcgtattag gcagtgagat
    10681 cactgatacc cttcgtgaac agctaagcgg gcgtttaggg ctagtctttt ctcaacaaaa
    10741 tgagatggaa gaaaacgcgg aatttagctt tttgacaagc gaactagtcg atccgctgga
    10801 aaaagaagca accggcaaac tattgactta tttatcggtg acacaaaagc ggagtttagc
    10861 tcatatccaa aaagcagtag aataccaacc agatcatttt ttgaaaatgg attattattc
    10921 aaaattcaac ctagaattaa gccaatctat tcggactggg aaaaagcacg gcactcttct
    10981 atggttgtta gatgaaacaa aaacagcaat gggtggccgt ttactaaaac aatggctaga
    11041 ccgtccgctt atccaggcaa aacaaattaa agcaagacaa gaaatggtcg cttctttgct
    11101 agacagctat tttgaacgta tcgatttgca gtcagcatta acgaaagtct atgatctgga
    11161 acgcctagca ggtcgagtag ctttcggtaa tgtcaatggg cgagacttga tccagctgaa
    11221 aacttcttta gaacaagtgc caatgatcag agggttgatt gaaggaatcg atcaaggtca
    11281 atggcaaagt ctgctttcag aaatgcagcc gatggaccat ctcgtccaat tgatcgacca
    11341 agctatccaa gatgatccgc cgttacaaat aacggaagga aacatcatca aagatggatt
    11401 caatgagcag ctcgatatgt accgttctgc tatgagaaac ggaaagaaat ggttggcaga
    11461 acttgaagca aaagagcgtc aagaaacagg aatcaagaac ttaaaaatcg gatttaaccg
    11521 cgttttcggt tattatatag aaattacaaa agcaaatctt ggaaatgcag aactggagaa
    11581 gtatgaacga aaacaaactt tagcaaatgc agagcgtttc atcacaccag aactaaaaga
    11641 attggagaca caaatcctcg aagcagaaga aaaatctgtt gatttagaat atcagttgtt
    11701 tttagctgtg agagaagaag tcaaaaaggc tatccagccg ctacaagtac ttgcaaaagc
    11761 aatcagtgca gtagatgtgc tacaaagttt tgcaacgatc agcgaacgct atcagtatgt
    11821 tcggccagaa cttgtcagtg acaagcatca gcttctgatc aaagacggac gacatcctgt
    11881 agtagaaaaa gtattaggac atcaggaata tatccctaac agcgtagaaa tggcagaaga
    11941 cgaaatgatc ttgctgatta caggaccgaa tatgtctggt aaaagtacgt atatgcgaca
    12001 gttggcatta actgttctga tggcgcaaat gggctgtttc gttccggcaa aacaagcagt
    12061 tttacctatt tttgatcgga tttttacccg aattggtgca agtgacgact taattgctgg
    12121 acaaagtact tttatggtag agatgatgga agccaatcaa gccctgcgtt atgctacgcc
    12181 aaacagtttg attttgtttg acgaactagg gcgaggaaca gcaacctatg acggaatggc
    12241 gttggcccaa gcaatcatcg aatatatcca tcgtcatgta caagcaaaaa cactcttttc
    12301 tactcattat catgaactga cggtacttga gaaagaactt ccgcagctta aaaatgtcca
    12361 tgttggagcc gttgaaaaag acggagaagt tgtcttcttg cataaaatga tggatggacc
    12421 tgcggataaa agctacggca tacatgtggc aaagatcgca ggattgcctt ccaatctact
    12481 agaacgagca gcagatatcc tgcatacgct agaaaatggc gataacggac accatactgt
    12541 tccagctgaa gtagaagaga aaactaaaac agccaaacag gagcaaataa aaccacctgc
    12601 agaaaaagta aatcaagacc atatacaaga ccgtgtaaaa gaagaaaacc aacagctttc
    12661 tttattcagt gaattatccg aaaatgaaac agaagtcatt gaatcactga agcaaataaa
    12721 tttactagaa atgacgccaa tggatgcact aaacacatta tatgaattac aaaaacaact
    12781 gtaggaagaa ggcggatatc gatggccaaa atccaagaat tatctgaacg tttagctaat
    12841 cagatcgctg cgggagaagt agtggagcga ccagcttccg ttgtgaaaga attagtggaa
    12901 aatgcaattg acgccggcag tactcagatt gatatcttag ttgaagaagc gggactgcgg
    12961 acgatccaag tgattgataa cggagaaggc attttggcag acgacgtaga gaatgccttc
    13021 aagcggcatg cgacaagtaa gatccataat cgcgacgatc tttttcgcat acgaacgctg
    13081 ggctttcgag gggaagcttt gccaagtatt gcctctgttt ctgaaatgac tctggaaacg
    13141 gcgaatcagg aagaaaaaca aggcacctat ctgtcgctga aaggcggaga agttattgag
    13201 catcgaccag caccactgcg tcaaggaacg aagattaccg tcagcaatct gtttttcaat
    13261 acgccagccc gcttgaaata cgtgaaaaca ctgcacacag aattagcgaa catcggggac
    13321 atcgtcaatc gactggcatt gagtcatcca tcgattgctt ttcgtttagt acatgatgga
    13381 aacaaaatgc tggcaactgc tggaaatggc gatctgaaac aaacaattgc gggaatctac
    13441 ggattatcta ccgcaaaaaa aatgctgaaa atcgaaggaa aagatctaga ttttgagtta
    13501 tttggctatg tttcattgcc tgaggtgact cgtgccagta ggaattactt atcgacgatc
    13561 atcaatggac gttttatcaa gaacttttcc ttgaataaag caatcgttgc aggttatggg
    13621 tcaaaattaa tggtcggtcg tttccctatt gctgtattgg aaatcaaaat ggacccgctt
    13681 ttagtagatg taaatgttca tccgacgaag caggaagtcc ggctgtcgaa ggaaaaagaa
    13741 ctaacagagc taattagtaa cgccattcaa aaagcattgt ctcaagaaaa tctgattcca
    13801 agtgcagcgg ataatctacg gtttaagaaa aaagtacctg atcagcctaa aacagaacaa
    13861 atcgaaatcg atttgtccta cgcttttgaa gaagaaaaac ctaaaaagcc aagtagtttg
    13921 aattttgatc gacagacagg tacctttttc gtagaagaac taaagaacac atttgttgac
    13981 agaattgttg ataagtctgt ggaaaatgtt gaaaactatt ctggtttttc tgaagaaaac
    14041 accacttatc cagaaaaaac agcgaaaacg gtggataagt ttggtgaaaa tgttgataac
    14101 tttgtagaaa aaacagtgga aaaatctgaa aagaaagaat ttcccattgt ggataacatg
    14161 gagaaaacaa atgaagaaca agcagataaa tccgtagaag aggatgaaaa gatttacgag
    14221 gaagaattga cactccatcc tgaatttgat ttcactcaag gaaacgacaa aacattagaa
    14281 caagccatca agaaattaga aacggaaaaa ccagtacaac gttttcctca gttggaatat
    14341 tttggacaga tgcacggaac gtatcttttt gcccaaagta gcgatgggtt gtacatcatt
    14401 gatcagcatg ctgcccaaga gcgcattaag tatgaatatt tccgggaaaa aattggggaa
    14461 gtatctgatg atctgcaaga attgcttgtt ccatttgtgt tagactatcc aaacagtgat
    14521 gctttgaaat taaaagagca aaaacacttg ctagaagatg cagggattta tttagaagaa
    14581 tttggtcaaa acagctttat tgtacgggcc catcctactt ggtatcccaa tggagaagaa
    14641 gaatcgatta ttcgggagat gatcgatatg ctgctgacaa ctggaagtgt gagtgtaaaa
    14701 aaattccgtg aagcaacagc aatcatgatg agttgcaaac gatcgattaa agccaaccat
    14761 tatttgaatg aagcacaagc acgtgtgctg ctgaaggatc tggcacgatg cgaaaatcct
    14821 ttcaattgtc cccatggccg tccagtattg attcacttta ccaattcgga tatggaaagg
    14881 atgttcaaac gaatccaaga cccgcattaa gtcaaagaga agagggaaaa catgaagatt
    14941 gtattagcat cccaatcacc aagaagaaaa gagttattgg ctcatatcgt ttcagaattc
    15001 gagattgaac ctgctgatat cgatgaaacg ccatttccag acgaacagcc cgtcgattat
    15061 gttcgtagaa tggctgaaga aaaagcacga actgtctggg aacaagatga aaaagaagac
    15121 catttagtga ttgccagtga cacgactgtt gttctcaatc aagagatcat gggtaaacca
    15181 gaagatctgg ctgaggctga attcatgctg aaaaaactaa gcggggaaac acatactgta
    15241 tatacagctg ttgttttaaa aacaaaggaa cgagaagaac ggatactagc agaagcacat
    15301 gtcacttttt atcctttgac agacgaagag atcaaacgct acttggagac gggggattat
    15361 gcggataagg caggggcata cggcatccaa aatgctgcga gtgtattcgt caaagaaatt
    15421 tctggagatt attacagtat cgtaggattt ccgattggtg ctgtcaatca ggcactgaaa
    15481 acattcattt aaacaaaaaa gcggactatc tgattttcga ttgaaaatcc atgatagccc
    15541 gtttttttta ttgaaaagat tacttatcta ctctgactaa gattttactt cagctttacc
    15601 gactgcttcg cccgggcgaa caagtcctga ttggtcaaga gaaagtcctg caactttttc
    15661 attattggtc aaagcaacaa taatgtcagt tttcttgcca gcttgttcta tttctggcaa
    15721 atccatttca gcaatcaggg tctctggtgt gacaaggtat ccttctttca cgaatacatt
    15781 gaaaggaaca ccctttagtt caacagtatc taatcccatg tgaacaagta cttccagccc
    15841 ctcattagaa agaataccaa tggcatgttt tgtttcaaag acactcgtca ctttaccgtt
    15901 tactggtgca tagactttgc cgtttgacgg ttcgacagca taaccgtcac ccatcatttt
    15961 ttgtgagaaa actgggtcat ttacttcagt tatttccttg atggtccctg cagcgactgc
    16021 gaataatgtt tcttttgttg caactgccgt accagttgct gaaacaggtt cgaaagactg
    16081 tggcgttgct gttgcgccat ttgctgtttg aagttgtaca tcagcagcat tttcaggaac
    16141 agcaggatca atcttattca aaattccctg acgtctaaag atgatcgtca agccaaatgg
    16201 aatgatgaca gcaatgatca ttgcgatgat gaaaggtacc catgtttctg cttggatagc
    16261 tagtattcct ggcaaaccgc ctactccgat cgcgttagct cgaacaccca tcaagttagc
    16321 aaacatgcca gctaatccag agccgaccat tgcagcgacg aaaggataga cgtatttcaa
    16381 attgattccg aacatagcag gttcagttac gcctaagtaa caagaaatca ttgctggaat
    16441 cgagatttgt tcttcttttt tattcccacg atgtaagaag atgatggcta agactgcaga
    16501 gccttgggcg atatttgata aagcaatcat cggccataaa ttcgttgatc cgaaatctgc
    16561 aatcagctgc atatcgatgg cattggtcat atggtggaga ccagtaatca ctaaaggagc
    16621 atacaggaat ccgaatacgg cactaaacaa ccagctgata gaagacgtca atccagtatt
    16681 tacgatattt gagatccagc ttccgactgt ccagccgata ggacctaaaa taacgtgtgc
    16741 agctaaaaca gttggtaaca atgaaaacaa tggaacaaaa atcatcgaga tcgactgagg
    16801 gatatatttg cggaagaaga tttctagata cgctaacata aaccctgcca gcatggctgg
    16861 aataacttgc gcttgataac caatcatctg gacttgagca aaaccaaaat cccaaaatgg
    16921 aatatctgct gcggctgtgg aagctacact ataagcattt aataattgcg gagaaactaa
    16981 cgtaattcct aaaacgattc ctaatatttg tgtagtaccc atcttttttg caatactcca
    17041 agtgatacct actggaagaa aatggaaaat tgcttcaccg ggtaaccaca aaaaggcatt
    17101 gacaccattc caaaacttcg agtgttcaac gattgttcca ccaagacttt caaactggat
    17161 accttcaagt acatttctga atccaaggat caatccaccg acaatgattg caggaatcag
    17221 tggtgtaaag atttctgcta aaacggcaat agcacgttga acgggatgaa ggttttgttt
    17281 tgcagctgct tttccttgtt ctttggaaac cccttcaact ccagatactg caacgaagtc
    17341 gttataaaaa gtagaaacat cgtttcctat gatgacttgg aactgtcctg cattcgtgaa
    17401 catccctttt acgctaggta tatcttcgat cgctttttcg tccgcttttc ctggatcatt
    17461 caggacaaaa cgcatacgag tcgcacaatg actaactgca gcaatatttt cttttccgcc
    17521 gatatcatgt aacagcttct cggcatctgc ttgatatttt gccatatcaa taaacccctt
    17581 tctcttaact tgtctatgca agttgtttct tcatttacta tacttttttt cggagagaat
    17641 tgcaagcgtt ttattaaaaa gtacgagaga aaatgatatc gttttaaaaa gcttaaaata
    17701 agcttttctt aaaaatagga acattgaaat acaaaaagag ctgtagtata gtgaagaaat
    17761 gatggaagaa cgagtgaggg aaaaaacatg aataagttca atgaaatttt tttagatttg
    17821 gaacagaaaa ttttagcgaa agaatatcct ccgcattcgc ttttacccag tgagaaccaa
    17881 ttgatcaaaa tttatggtgt ttcacgagaa acaattagaa aagcgctgaa tcttttgact
    17941 acggaaggtt atattcaaaa aaaacaagga aaaggttcga tcgtactgga tcggaatcgc
    18001 ttcgactttc ctatttctgg attgactagc tataaagagc tacaggagac ccaacgtatt
    18061 ccaagtgaaa cgatcatcca tacgttagaa gaaacagaag tgacaaaagc attgaacgag
    18121 atcactggct gggagatcgg tgcgcctgtt tggcatttga tacgcgaaag aaaaatcaat
    18181 ggcgaagtag ttatattaga caccgattat ctattaaagg aaatcgttcc tattttaact
    18241 ccagcacaag cacaaggatc gatctatgaa tattttgaaa atgagctttc tttgacgatt
    18301 gcttacgcgc aaaaggagat cacagtagaa gaagtaacgg aaatcatgaa aacagcgatg
    18361 gatcttcacg atgacactta tgcagtagta gtcagaagcc tcgttcatct ggaagatacc
    18421 cgctgtttcg agtattctga gtcggttcac cgattagata aattccgttt cgttgagttt
    18481 gcccgacgcc gaaaagtgta aatagttcaa aagaaagcaa attgttggca tctgtccact
    18541 ttggcgctat gataggatca caaagggggc atttttaatg gataaaccaa taagaccaga
    18601 aaaagaagaa ctgaaagaaa agttatcacc catggcttat gcagtcacac aagaaaatgc
    18661 aacagaacga ccatttagcg ggaaatatga tgatttttac gaaaagggga tctatgtaga
    18721 tatcgtcagc ggtgaaccgt tattcagctc tgcagaaaaa tatgatgcag gttgtggctg
    18781 gccatcattc agcaaaccaa taacgaaaag acaagtgcgc gaaaaagccg atttttctca
    18841 tggcatgcat cgaatagaag ttcgaagcaa agaagcagat tctcatctcg gacatgtgtt
    18901 taccgatgga ccagtagatc aaggtggtct acgttactgc atcaattctg cctcactgaa
    18961 attcattcca tatgaaaaga tggatgaatt aggttatggg gtattcaaat cacttgtaga
    19021 ataatggaaa tacacaaaga gcggtacaat acggggattt actcgatcgt ccgctctttt
    19081 tttattcaag cgattgctat ttgaaagaaa cgctccctag actccttttt tcacaaggtg
    19141 aaatgtgcta caataaaggg aacgtatgtg tgcaaaagaa agaggactag aatatgtatg
    19201 aatatctgac aggtgtggtt acctttatca acccctatta catagtagta gaaaccaatg
    19261 gtatcggcta ccagatcgca ctaggaaatc cttaccgcta cagtagcaaa ctaaatcaaa
    19321 aaataaaaat ttatgtccat caggtaatac gtgaagatgc gcatcttctg tatggatttg
    19381 attcgctaga agaaaagcaa ctgtttttgc gattagtaag tgtttcagga atcggtccga
    19441 aaagtgccct tgcaatcatg gcaagcgaag accactccgg tttgattcaa gcagtagaat
    19501 ccagtgatgt cacttatttg acgaaatttc caggagtagg gaaaaagaca gcacaacaaa
    19561 tgatcttgga tctaaaagga aaattcggtg agctgagtat cgatacacct tttagtctat
    19621 ttgatgaagc aaacacaaaa gacgctacag ctctatctga agcaatggaa gctctctcgg
    19681 ctcttggtta cagtgatcga gaaatcaaac gtgtagaaaa acaattaaaa gaagtagata
    19741 atcagacaac ggatgagtat ctacgccaag cactcaagct aatgatgaaa aaataaggag
    19801 gagaccaaat gacagatgaa tacttgttat caccagaatc tggcgaaaat gaactgagtt
    19861 tggaaaaatc tcttcgacca cagctgcttt ctcaatatat cggacagaga aaagtcaaac
    19921 aagaacttca gatttacatc gaagctgcca gaaatcggga agaaccttta gatcatgttc
    19981 tgttgtacgg gcctcctgga ttaggaaaaa cgacgatggc catggtcatt gctaatgaga
    20041 tgaccgtaaa tattcgtacg acgagtggcc ctgctatcga acgtccgggt gacttagtag
    20101 caatcttgaa tgaactggaa gcaggggatg tcctgtttat cgatgagatc catcgattac
    20161 ctagagtggt agaagagatg ctttattcag ctatggaaga tttctatgta gatatcatgg
    20221 taggacaagg gccaaccgca catccggttc atttccctct tcctcccttt actttgattg
    20281 gcgcgacgac acgggcaggt atgctttctg cacctttacg agatcgattt ggcatcattt
    20341 ctcatatgga atattatgac gaacaggatc tgcaggagat cgttttacgt tcttcggata
    20401 tttttcaaac agagatcatt gaagaagggg cattggaaat tgctcgccga tcacgaggaa
    20461 cgcctagaat cgctaatcgc ttgctcaaac gggttcgtga ttttgcgcaa gtccaaggag
    20521 acgggaaaat cgatcgtcct atagcggatc aggcacttac tttgctacag gtcgatcagg
    20581 ctggattgga ttatgttgac cagaaattgt tgcgaacaat gatcgagtta tatggcggcg
    20641 gaccagtagg gttaagtacg ctttcagtaa acataggaga agagcgtgag acggtagaag
    20701 atatgtatga accgtatctg atccaaaaag gatttttaaa gcgtacacct agagggcgaa
    20761 ttgctactgc ttatgcctat gaacattttg gttacgacta tcatatatga tttttgcttt
    20821 tagattggag gaaaatgaat gagtgataca acacgtacga aacaattgat caaaaacgta
    20881 ttgattgatt tgtgccaaac gcaatctttt cgaaaaatca gcgtccaaaa aatcgcaaac
    20941 gaagcaaaaa tcaatcgaca aacattttat tatcatttta cagataaata cgatttgctt
    21001 cgttgggtct attgtgagga tgcattgcat tatttagatc cacaatgtct ttcacttgat
    21061 aattgggaag agcaagcatt gaaaatgctg aaagcaattg ctgagaagaa agaattttat
    21121 gcaaatactg tgctttctga cccagaaatt ttacaaaaag agttttctca actgatccaa
    21181 aaatcattta tcaagatttt tgaacaaatg gatgaagaaa gacaactttc tgcaagagac
    21241 aaactatttt atgctcgttt tttttcttac ggttgcagtg gtgtattagt caattggatc
    21301 acgaatgaat atgaagaaac accgatggaa atcgctatgc agcttttccg tttagccaag
    21361 gatacggaat tatattctta tcgtctttat gcaaaagaag agttggaaga acagtaatag
    21421 aaaaattttt tgttggaaaa gcaataatcg ctaaaaggag atggggaaat gacaaaaatt
    21481 ttatttgttt gtttgggaaa tatttgccgt tcaccgatgg cagaaggctt gctgagaaaa
    21541 cgaatagcat tagaaggaag ggaagcagaa ttttttgtgg attctgctgc tacaagcacg
    21601 tatgaagttg gaaagacacc gcaccctgga acgaaaaaaa tattaaacca agaaaaagta
    21661 gatatgacga atatggttgc tcggcaaatc acaccccacg attttgaaac ttttgattgg
    21721 atcattggca tggatcaaga aaatgtagaa gaactgaaac gaagagcacc acgctcagcg
    21781 caggggaaaa tccatttgtt tttaagtagc attcctggaa aagaaaaaga aaatgttccc
    21841 gacccatact ataccaataa ctttgatgag acatatcact tgatcacaga aggcctgaat
    21901 cattggtgga atatttggat tgatgaaaat aaaataacaa acaatttgta aagttaacgc
    21961 tttcttttta aggcgatgtt agagcatttc cttgtttttt tgtaaaatgt taggtaaaat
    22021 aaaattaatt aagtaacact ttcttagcta aaggaggagt gcattcgatg aacgaagaga
    22081 cagtatcgcc aatgaagaag cttacgaagt tagttactca tacttcgtat caactttttg
    22141 attcttttgt aaatgacact gaagaacatg ctaagaatgt acgattgcaa ggaaagttta
    22201 ttcttgagct gcaaaaagct gctactgaac atagtctagt tatcttacaa gtaagagcaa
    22261 atcgtttgag tagcaagtat gaaacgatct caggctgggt ggcgacaaaa acggtcgaaa
    22321 aagaccgagt gatgatccgc ctacaaaacg aagagcagca gatccggatg gttcctgtag
    22381 aacaaattga aaaaataaca atcctttcac cagcagggga acaggaacgg ttatcaaaat
    22441 aatttatacc agatagaaaa gcacgtatga gcttgtacca tcaatcatta ctaatcaaaa
    22501 ataaccgaat gctttaagct gatatcagta aaacggaacg ttttaggatg agtatcatag
    22561 catgacagca ttcggttgat tagttgaatc gattggatgg tacaagtttt tttgataaaa
    22621 aaaggtcctg ctagccgggg agctagcagg ataaggagtt aaaaatgaaa aagtgttgtt
    22681 agggttgttt gttggtatgc ttatataata gtcattagtt gtgaatattt tgtgacgaaa
    22741 gttttcgaaa gttgttaagt ttt
//
LOCUS       DASJSZ010000046        22633 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.046, whole genome shotgun sequence.
ACCESSION   DASJSZ010000046 DASJSZ010000000
VERSION     DASJSZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.046"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(227..1114)
                     /locus_tag="U4B03_001609"
     CDS             complement(227..1114)
                     /locus_tag="U4B03_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2068464.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="U4B03_001610"
     CDS             complement(1111..1980)
                     /locus_tag="U4B03_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2068465.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="U4B03_001611"
     CDS             complement(2117..3403)
                     /locus_tag="U4B03_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2068466.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="U4B03_001612"
     CDS             3676..4254
                     /locus_tag="U4B03_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2068467.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="U4B03_001613"
     CDS             4254..5183
                     /locus_tag="U4B03_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2068468.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="U4B03_001614"
     CDS             5180..6916
                     /locus_tag="U4B03_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN2068469.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="U4B03_001615"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="U4B03_001615"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEN2068470.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="U4B03_001616"
     CDS             complement(7812..8321)
                     /locus_tag="U4B03_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEN2068471.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="U4B03_001617"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="U4B03_001617"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN2068472.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="U4B03_001618"
     CDS             complement(9622..10713)
                     /locus_tag="U4B03_001618"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEN2068473.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="U4B03_001619"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="U4B03_001619"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEN2068474.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="U4B03_001620"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="U4B03_001620"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEN2068475.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="U4B03_001621"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="U4B03_001621"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN2068476.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="U4B03_001622"
     CDS             complement(14676..15650)
                     /locus_tag="U4B03_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEN2068477.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="U4B03_001623"
     CDS             complement(15631..17514)
                     /locus_tag="U4B03_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEN2068478.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="U4B03_001624"
     CDS             complement(17532..19757)
                     /locus_tag="U4B03_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN2068479.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19872..20906
                     /locus_tag="U4B03_001625"
     CDS             19872..20906
                     /locus_tag="U4B03_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2068480.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20973..21986)
                     /locus_tag="U4B03_001626"
     CDS             complement(20973..21986)
                     /locus_tag="U4B03_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2068481.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22064..22252)
                     /locus_tag="U4B03_001627"
     CDS             complement(22064..22252)
                     /locus_tag="U4B03_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEN2068482.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         6446 a         4863 c         4096 g         7228 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
    19861 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
    19921 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
    19981 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
    20041 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
    20101 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
    20161 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
    20221 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
    20281 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
    20341 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
    20401 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
    20461 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
    20521 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
    20581 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
    20641 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
    20701 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
    20761 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
    20821 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
    20881 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca
    20941 tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat
    21001 gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta
    21061 atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac
    21121 cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac
    21181 gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc
    21241 gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata
    21301 aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa
    21361 atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac
    21421 gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca
    21481 ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct
    21541 ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca
    21601 taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg
    21661 acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca
    21721 ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata cttggactca
    21781 tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca
    21841 gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa
    21901 agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg
    21961 ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg
    22021 attataccat agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa
    22081 acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat
    22141 tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca
    22201 gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat
    22261 ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga
    22321 acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga
    22381 gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa
    22441 tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc
    22501 tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc
    22561 attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa tcgccaaaat
    22621 aatgtagact aac
//
LOCUS       DASJSZ010000047        22526 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.047, whole genome shotgun sequence.
ACCESSION   DASJSZ010000047 DASJSZ010000000
VERSION     DASJSZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.047"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..210)
                     /locus_tag="U4B03_001628"
     CDS             complement(<1..210)
                     /locus_tag="U4B03_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068483.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
     gene            complement(301..753)
                     /locus_tag="U4B03_001629"
     CDS             complement(301..753)
                     /locus_tag="U4B03_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2068484.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(766..1176)
                     /locus_tag="U4B03_001630"
     CDS             complement(766..1176)
                     /locus_tag="U4B03_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2068485.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1451..1663
                     /locus_tag="U4B03_001631"
     CDS             1451..1663
                     /locus_tag="U4B03_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2068486.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1666..1878
                     /locus_tag="U4B03_001632"
     CDS             1666..1878
                     /locus_tag="U4B03_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068487.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2018..2173
                     /locus_tag="U4B03_001633"
     CDS             2018..2173
                     /locus_tag="U4B03_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068488.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2170..2394)
                     /locus_tag="U4B03_001634"
     CDS             complement(2170..2394)
                     /locus_tag="U4B03_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN2068489.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2472..2660
                     /locus_tag="U4B03_001635"
     CDS             2472..2660
                     /locus_tag="U4B03_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068490.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2986..3321
                     /locus_tag="U4B03_001636"
     CDS             2986..3321
                     /locus_tag="U4B03_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068491.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3554..4495
                     /locus_tag="U4B03_001637"
     CDS             3554..4495
                     /locus_tag="U4B03_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEN2068492.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4497..5387
                     /locus_tag="U4B03_001638"
     CDS             4497..5387
                     /locus_tag="U4B03_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEN2068493.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5430..6230
                     /locus_tag="U4B03_001639"
     CDS             5430..6230
                     /locus_tag="U4B03_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN2068494.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6245..7096
                     /locus_tag="U4B03_001640"
     CDS             6245..7096
                     /locus_tag="U4B03_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2068495.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7297..7758
                     /locus_tag="U4B03_001641"
     CDS             7297..7758
                     /locus_tag="U4B03_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2068496.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7784..8236
                     /locus_tag="U4B03_001642"
     CDS             7784..8236
                     /locus_tag="U4B03_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068497.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8342..8536
                     /locus_tag="U4B03_001643"
     CDS             8342..8536
                     /locus_tag="U4B03_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068498.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8567..8953
                     /locus_tag="U4B03_001644"
     CDS             8567..8953
                     /locus_tag="U4B03_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068499.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8950..9153
                     /locus_tag="U4B03_001645"
     CDS             8950..9153
                     /locus_tag="U4B03_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEN2068500.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            9150..9446
                     /locus_tag="U4B03_001646"
     CDS             9150..9446
                     /locus_tag="U4B03_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068501.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9522..9938
                     /locus_tag="U4B03_001647"
     CDS             9522..9938
                     /locus_tag="U4B03_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN2068502.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10199..10336
                     /locus_tag="U4B03_001648"
     CDS             10199..10336
                     /locus_tag="U4B03_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068503.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            10746..10976
                     /locus_tag="U4B03_001649"
     CDS             10746..10976
                     /locus_tag="U4B03_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068504.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            11135..11968
                     /locus_tag="U4B03_001650"
     CDS             11135..11968
                     /locus_tag="U4B03_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HEN2068505.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11961..13376
                     /locus_tag="U4B03_001651"
     CDS             11961..13376
                     /locus_tag="U4B03_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEN2068506.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13388..14917
                     /locus_tag="U4B03_001652"
     CDS             13388..14917
                     /locus_tag="U4B03_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2068507.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14835..15887
                     /locus_tag="U4B03_001653"
     CDS             14835..15887
                     /locus_tag="U4B03_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEN2068508.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15889..16206
                     /locus_tag="U4B03_001654"
     CDS             15889..16206
                     /locus_tag="U4B03_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068509.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16333..16956
                     /locus_tag="U4B03_001655"
     CDS             16333..16956
                     /locus_tag="U4B03_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEN2068510.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16971..17861
                     /locus_tag="U4B03_001656"
     CDS             16971..17861
                     /locus_tag="U4B03_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HEN2068511.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17884..18099
                     /locus_tag="U4B03_001657"
     CDS             17884..18099
                     /locus_tag="U4B03_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068512.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18111..18452
                     /locus_tag="U4B03_001658"
     CDS             18111..18452
                     /locus_tag="U4B03_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068513.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18452..18823
                     /locus_tag="U4B03_001659"
     CDS             18452..18823
                     /locus_tag="U4B03_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068514.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18816..19211
                     /locus_tag="U4B03_001660"
     CDS             18816..19211
                     /locus_tag="U4B03_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN2068515.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19213..19590
                     /locus_tag="U4B03_001661"
     CDS             19213..19590
                     /locus_tag="U4B03_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068516.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19591..20199
                     /locus_tag="U4B03_001662"
     CDS             19591..20199
                     /locus_tag="U4B03_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2068517.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20199..20540
                     /locus_tag="U4B03_001663"
     CDS             20199..20540
                     /locus_tag="U4B03_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068518.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20782..22526
                     /locus_tag="U4B03_001664"
                     /pseudo
     CDS             20782..22526
                     /locus_tag="U4B03_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT         8131 a         3290 c         4641 g         6464 t
ORIGIN      
        1 attaatatca ttttttaaca tagtaatttg tttatttaaa tctgatagtg tttcttcctt
       61 acttaatata aatctttctc tatcatgtaa tttatttagt ttttcatcat ttttttgttc
      121 taattcataa gcttcttttt ttattgtgtt agctttcgac agtttagtga ttcctataat
      181 taataaaata agtccaatca ataataacat tcaaaactcc tcatttcttt gatataatta
      241 ttgtagagtt tactcagaaa tgaggaaaga gtccatgtga aagcatgggc ttttttattt
      301 ttaatgttgt aatactaaat gttcaagatt atgaggaagc cctaaatatt caataatttg
      361 gaactgactc atatcagata aatcttgttc atctattgtt ctagttaaca tttcaattgc
      421 aaacatatta gcttcagctt ctataccata aatcatacaa ctaaggttgt ttgctctaaa
      481 agctgctgtg caaatacctt tatggagtct aacgtgggct atttcatgcc ataatacaaa
      541 tcttttcata gaaggtgata ggttttgatt aattaatatc acataacatc ttttattgtg
      601 tatcgtttgt cctaatgttt tattccctaa atcagtttca attaaatcta tatcagaata
      661 tttgattatt ttatatggat taatcgcatc agatccaaac tctacaaata atttttctat
      721 ttcttctaat atccagtatt tgtttaaatg cataccaaag cccacctatt gacgatattt
      781 tttaggagtg aattttttct ttgctttaac tttggttaag gcaagtgcaa gttgaatggc
      841 atcgtataat ttttccttat cttcatcgtc taaaggttca ccataataat ttgtttcagc
      901 atctgaattc aagccttcaa gtaatttttc agcttgtaaa gcaatatctt gtttatcttt
      961 ttctgttaaa tcataataat ggcgtttatc agtacgtcct aaaagataat cagttgatac
     1021 attaaagtaa tctgcaactt tttgtatttt atctccagat ggagtacttt tattccatct
     1081 agacatgctt ccatttccga aatttaactt tctttcaagt tctgccaacg tcattgaacg
     1141 ttcttttaat aagattttta tacggtctaa tagcatagga tatgcattcc tttcaaaata
     1201 ccgacaaaaa aagtattttt ctgcaaatta tctatttaca aatagagaat ttgcagttat
     1261 actagttttg taagcaagat atttacggca tacaaagact aaaagcaata aactaatagt
     1321 ctccccagac atcagtaagc tcgctttttg tgcgctgtat tttgtatgct tacataatag
     1381 catatttgca gtttttgtca aacttattag acaaaaatat tagaaaatat tcgataaggg
     1441 ggtatgaagc atgactttaa aacagcaagt attaattgct ttagtaaaga aaggttggag
     1501 tcaaagagag ttagctcgac gaatgggtat atcaattact tatttacgag atatcttcat
     1561 cgaaaaaaga aaacctaagg aacgtttaaa acaaatcgaa gaacttttgg atatcaagtt
     1621 agaagtagat tctaatgatc agccatcaga acaggaggcg taaaaatggt acaacgtttg
     1681 agcaaagcgg agtttaaaga gaaatataag tattctgaaa gtacttatca aagaagaatg
     1741 aaggaattta aaaaatcacg ttttagtgag gggtataacg cagttacttc caaagaaata
     1801 acaattgatg tcgatttata tgaacagttt agaacatggc gttctaaaaa tcgatttaga
     1861 gttagagctg tttgttaata gctaaaaaat ctaaattcac cacttctgaa aagaggagtt
     1921 gctgctctcc tttcaagttt gccaaagtcc acgttttttc attttggcaa ctcctcttct
     1981 tggaggtggt gacggcgaga gacgaaggag ggatgacatg aaggaagaat caaaaatagg
     2041 gattttgaat gagaacaatt acacaagatt aattattgaa acagaagaag ggaaaaaagt
     2101 agcagaagtt acattgaccg atgcaactcc tgctgacggt tatcgtattc gattaactcc
     2161 taaatatgat taaccttttg gtgggtgtgg atcgtttccg tgactatcac ggctttgaat
     2221 ttttccatct ctgccatgaa taaatagctc agatccttga tttttagaaa tctctctagc
     2281 gatttttact gcttctgatt ttgtattagt atgtgcagtc gctttagaat ttccagcacc
     2341 tttgacattc caaccaccat tggaattagg tacaacatgt tgattttttc ccatagtttt
     2401 cacctccatt tctatagaaa tattttatca aatagaaatg gagaaattca ataaacgaaa
     2461 ggagaagtga tatggcatat accttgcaac aagaacatca aattctcggt ttgattaaac
     2521 aacgcaggaa acaattacaa gatgaccgtg cagcgcttag aaaagccgat gagctatcag
     2581 ataggcaagc tgaactaatt gcttctgaac ttgaggattt gagaatgcta gaaataaaaa
     2641 atagggagat tagattatga agaagacaga cacacttttt ataggattca ttttggggtt
     2701 attagtgatt gtagcgcacc aaagtattat cgggggaagc ttgttcgcag cattgatggt
     2761 tttaatcaat ctgcttgatt caaaagaaag gagcaactat ggcacgagaa gaagcgctaa
     2821 aaatcggtaa agtgattgct gataatcggt ggggaaatag ccgccctatt attttaagca
     2881 agcaacatat cgaaaagcaa aaagcatggc aacaaataaa aaagtgactc tgccagccag
     2941 caaagagtca catacaaatt tataaaacta ggagaattat atcacatgaa taagaaaatt
     3001 gaaaatctta tcgaagaatt gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt
     3061 acagcccaaa aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta
     3121 ctttgtcttg ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt
     3181 atgcgtagaa ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct
     3241 tcaaatcata cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt
     3301 ctcaaggggg aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt
     3361 tcacgttagt gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca
     3421 tgcttgtgct ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat
     3481 cttgacaact atttaactga tcctgattat gctaaaccac catatgaagc accaattgat
     3541 gaggaggatg aagatgagta aatctacctt agaaatgagc catcaagaat ggcttgaaga
     3601 ccgtaagaaa ggcatcggag gttcggatgt tggaacaatt ttaggattga ataaatggaa
     3661 atctccttat caactatggc tagaaaaaac aggacaagta gtacttgaag aatcaggaag
     3721 tgagcccgct tattggggta atgttttaga agaagtggtt gctaaagagt ttcaggaacg
     3781 tacaggcaaa aaagtacgca gaagaaacca agtatttgaa catccattac atccattttt
     3841 aagagcaaat attgatcggg aagtagtggg agaaaatgcc attctggaat gcaaaacagc
     3901 taatcaattt ctcggtaaag aatgggaagg cgaagaggta ccgcttagct atctctgtca
     3961 agttcaacat tacatgaacg ttctaaacaa agactattgt tacatcgctg tcttaatcgg
     4021 tggtcaaaaa tttatctgga agcggattga acgagatcaa gagctgatcg atacaatcac
     4081 tgaacaatta gtagagtttt gggaaacaaa cgttcttgga ggtatcgagc caattattga
     4141 tggtagtcaa gcgacagctg atttcttgaa agagaagtat gcagatgttg aagacgttca
     4201 aacgacgtta ccaattcatt tcgatgaact agttgaacag aaaaacgaac tcaaacggac
     4261 taagaaagaa attgaatcag ctatccgtca agtagacaac gagattatca gcgagctagg
     4321 taaacgtgaa gccagtatcg gtatcactca aaaaaacatc atcagttgga aatttgtccg
     4381 tacgagacgt atgaactcga agaaactagc agagaaatat ccagatgtcg caaatgatga
     4441 agagatttat aacgttactg aatcaagaag gctaaccgaa agggagatca aataatatgg
     4501 caacaaatga atcgttaaaa aatcaattgg cagcaaagcc acagaaacaa gttgcaccag
     4561 gtcaattagg acttaaagct ttgatgaata caccaacaat gagaaagaag ttcgaagagg
     4621 tgcttcatga caatgctaat gcttttatgt cgaatgttat gactcttgta tctaatgaca
     4681 gttatcttgc agatagtgaa ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat
     4741 taaatcttgg gctagataag aatttaggtt atgcatattt agttccattc aatagtaaaa
     4801 acaagcaaac aggaaaatgg gaaaagaaag ctcaattcat gcttggctat aaaggatata
     4861 tccaattagc ccaacgatca ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag
     4921 gagaactaaa aagctggaac cgactgacag aagagtttga gtttgatcca aatggtagaa
     4981 cgtctgatga agtcattgga tatgttggct atttcgagtt actgaatgga ttcaaaaaaa
     5041 ctgtctattg gaccaaacaa gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg
     5101 ataagacaaa gttaagtggt gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg
     5161 ttttgagaaa tcttctttct aaatggggaa tcttgtccat cgaaatgcaa gaagccacca
     5221 catcggatga gagagtccaa agggttcaag aagacggcag cattattgct gaaacagaag
     5281 ttgaagaaga tattcctgaa agaaaagaag cagaggttat atctgaagaa aatgaagatg
     5341 tacaaactgg attatttgat gcatctaatc cgccgttaaa caaataatga gggagctttc
     5401 tcccttgctt ttctagagga gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa
     5461 aagtgatgaa ttcattcgtt tggaccaatc cttatatgta taaattatgg aacttgtgtt
     5521 tgatgaaagc gagtcatgag aatcgcaaga tactttttaa tggaaaagag atacaagtga
     5581 acagcggaga attcgtcaca gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga
     5641 aacgtgaaca tcaagtgaac agcggttctg tatggagatg gctaaaacaa tttgaaaaaa
     5701 acggaatgtt gaacatcaaa tcaactacga aatacagcgt tatatcaata aaaaactggt
     5761 ctttgtacca aagcagtgaa caacaaatga acatcaaacg cacaacaagt gaacaacaag
     5821 tgcacacaaa caagaatgaa aagaattata agaatgaaaa gaataataaa acatcatcgt
     5881 cgcaaccacg caaaaagcgt gtttacgaca ccgactcagt ttactacatt ctcgcggagg
     5941 agttattcaa acagatttgt cagaatcagg aaatcaaaaa gccgaatctg caaagttggg
     6001 cagataacat tcggaaaatg atcgagatcg acaagcggac cgaaaaccag gtacggggaa
     6061 tgattgaatg gagtcagcac aatgtgtttt gggcatcgaa tattttatct gctaaaaaat
     6121 tgcgagaaaa atacgacaca atggcagcgc aagccaatcg tgattataaa acaaaacaaa
     6181 ctaaaacgct tgaatacgag aaatttagca cagatgagtt gcctatttga gaggaggcgt
     6241 aggcatggaa actgttgggg aaatcatgga aaagctgata cagaaagtgc ttgtccaacg
     6301 tggcgaatgt cctgaatgcg gacagccttt gtatggatgg cgtacgaaga accctgatgg
     6361 ctcagaacgt tgtaagccaa cctgtatgca atgtgggtat aaagcattgc gagttcagga
     6421 agacttacaa acagaacgaa tttacaacga gagcctgaaa gcaagagcaa tcaatttttt
     6481 caaaggtggt tctgttgtgc ctaatcaagc gttgtttgat tgcacattgc agaattatca
     6541 aattgtcgat caagaaacaa gacaagcggt tgaagtaacc aaacgctttg ttaattcagt
     6601 cttgttagga aatccaagtc accttgtttt aactggaaaa caaggaacag gtaaaagtca
     6661 cctagcgatg gcagcggctt gggaagtctt gaagcgatca aactacgata aaaaaatctt
     6721 atttatcggg ttacaggaaa tgctggatca aatcaaattt tcgtacaaca atcctgaact
     6781 cagaaaaacg attgagggat cgttgattgc agatatcaaa acagcggatt tagtcatcat
     6841 tgatgatatc ggttcagaac taggaaaaga tgcatcagat agtcgagcgt ttggcataaa
     6901 cacgctaaat tcgttcttgg atgcacgaca gaacctagca acgattatca caacgaactt
     6961 gcttggtgaa gaactgaaaa aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa
     7021 ctctgatgga tttacgatgg tattttctca aacagcagac aagcgcataa aaccagtcaa
     7081 aggagcgatc gcatgaattt gactaaacaa gaaaaaagaa tctgtcgtca gataatgttg
     7141 caaattatag gtgatcgtga catggacgaa ttatctcaac aagagagtcg aatggttcgg
     7201 aagataagta agctaattat ttatcgggat acagaaagat ttctcactgt tttaggtaac
     7261 gtctacgaga atccagagtt attggaggta gagtaattgc caaaaatatt agatgcatgt
     7321 tgcggtagta gattgttctg gtttgataag aacaattctg acgtaacatt tatggattgt
     7381 cgtcagcagt atgaagaatt atcaacaggt catgtgatca atgttgatcc agatgtggtc
     7441 gcagactttc gcgatatgcc ttttgaagat gatgtgttcg atatggttgt atttgacccg
     7501 ccacacttaa gacatgtcgg cgaaaactca tggcttgcca aaaaatatgg aaaattagac
     7561 gaactttggc cagaagacat caggcaaggt tttgcagagt gtatgcgagt tttacgacct
     7621 tcaggaacat taatttttaa gtggaacgag gaacagatac cactttctga tgtgttagag
     7681 gctattggag aacagccatt gtttggtaat aaacgaagca aaacacattg gcttgtgttt
     7741 atgaaaaaat tcagctaacg gaggaaacaa aggaggaaaa gtaatgaaga ttgtattaaa
     7801 taaatgtttt ggtggctttg ggttgagcca tgaagcaaaa atggaaattt ttaaaagaaa
     7861 aaatattgaa gtattcccat acatcaataa ctttagctat gactcagatg atgaatacac
     7921 gagatacaca ggccaaaagc taggttcgat ggattttatc tactatttca aaaaagaccc
     7981 gaaaatcgac aaagttacag gaatttatag cgaaattgac cgactgtacg gaattgctga
     8041 tgattccagt ttttcaagtg attcaaacag aggtgataag gacctcgttg ctgttgtcga
     8101 aaaactagga agtgaggcca gtggaccata tgcaagtctt aaagtcgttg atatacccga
     8161 tggggctgag tgggaaataa gtgattatga cggcgttgaa actgcacatt atggttttca
     8221 aacaggtagt atttagtcca ctaatgacaa ctaaagagga ggcagaagga tgagtgaaga
     8281 tgaatacttt gaacagtagt ctattaacga cagagatttt taactggaaa ggggtgtagt
     8341 catggacgca tcggaagtag taaataaaat taatagtctc aaagaggttt tcggagacgt
     8401 cgaagtagtg ataaacgcac aaggctatga caacggattt tttaagcaga ttaccgacat
     8461 tagcattcag caaggaatgg aagacgaaga tggaaacttt atcgatgaag tagccatttt
     8521 agtgacttgc gagtaagtaa ttgacagcta aagaggaggc agaaggatga aaaaattgca
     8581 ccaactcata tctgagaaag aaagcgaact ccaaaattta gaagatagtt tgggattggg
     8641 gtttccaatt gttgagcaag ctaaaatgac tcaaatcagt catctacgac ttgaattgga
     8701 agatttaaga caaatagaaa aatcaattca actcaacgac aaccagaaaa tcgtgttgga
     8761 gtggttgaaa agcgagacaa ttttaactag agaagcacca atattatctg ttaatgcttt
     8821 ttctgataaa aatttactag gaaaattacc tgataaagta cgcaaagctt ataaactatt
     8881 ggcttgtaaa caagaatatg aagtcctatc agcattcgct caatggggat tagaacagga
     8941 ggaagcggaa tgaacaacag acatcgcaga ataacaaaac taagaaaaca ggaactgaat
     9001 gtactaaaga caaagtttga aaaagaatat ggaatttcag cagaagaaac atataaagtg
     9061 gcaagtcagt ttgttgctga tgcgagtaat gctattcgta agtttgggat ttcgatatta
     9121 aatgatgatc gtaaatggga ggaaatgaga tgaaaataaa agacggattt tacgctagta
     9181 gtcatggtat cggcggttta atgctagaca tgccgataaa gaaccctaaa acacgtcaga
     9241 aatcaaaatt caaaatcggt gacatggtcc gatgtaaagc agaagagttc atctatccgt
     9301 ttcgtggata tgtagagcat gtctataatc attcagcaat cattcacatt gaaaacacga
     9361 tggaatgcga taagtggtta gcgaaaagca aagataattt agcagtggct cgtttggtgg
     9421 atatggaact aatcaatgac aaataaaaaa gccggatcgc tccgactgat tcaataaatc
     9481 caacacattt attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg
     9541 tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg
     9601 agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattattacg gatatgccga
     9661 aggcaccgaa gcacggcaat aaggcagaag acgcgatcat tcagatgatg gatatagaag
     9721 cggagagaga cgcaatacta gcggctttga tggctcttag tctgattagc cgtcagatac
     9781 tctactacag cttctgtgac gtaaacaagc actctaatta tgaaataggg caattgatac
     9841 gaggatacgg agagaagaat gtagagaagt tgaaatccat cgcattaatc gaatttgcag
     9901 aagcatacaa aaaaggcgtg ttagttcagt atcgttgatt ttgtagggtt tttgtaggga
     9961 tagtgtaggg tttttgagcg gtttaacgtg atattatggt agtgtcgaaa gattagtgat
    10021 aggtctgaga caaaataaaa tgtaagggag gaaatctccc tcatcgtttt aacttaagct
    10081 tcgatagaca gcaacggaaa tattaagaat aaggatgtga atttcaactc cttctaaatt
    10141 gttcttatta tctatcatcc gttgttgtct attaatttat gcatcggagg aaaaacgaat
    10201 ggataaagaa atcaaagtaa ctgtcaaatt agatttgact gaactaaaag aactgctcaa
    10261 caaggctagt gaccaagtcg aacagttaca agaaacttta gatgaaattg ctaattttaa
    10321 aatccaagtt tcttagcagt atatttttta gcagctgcac tcataaaatc ataccaagta
    10381 tcagaaaaat ataacttaac taaataatta aaaaaggtgg gtaaatagaa atgacggcgt
    10441 taataatttc aatttttgcg ctctgtctta atgtctatat gattggattt aaaaatggac
    10501 aaaataaaaa atagtagcag ccgagaataa ttttatagtg tcactgtggc ggaaatagaa
    10561 gacgcaaagg tggaggtagg aagcgtatgg cgcactatcg ggaccttgta caaaactaga
    10621 aaggactagt acgtgtgtgg tgcgagccct agggaggaac aggataaccg cctgtgtgta
    10681 ggttgctatt acatattaga tcactctttg agtggtcttt ttatttacat aaaggaggct
    10741 acataatgag aaactactgg tatatatcgc taactaatga atatcctcga accattgatg
    10801 attgttcagt gcgtgttgtg cgttctgtcc aaatcaaaaa gaagtactcc attgttgaaa
    10861 tgaccagaga agctacgcca aaagagattg ataaatgcaa acttctttat tgcggtcatg
    10921 gtttctatga tgaaccaaac attcaaaata atattaacaa gaatttgagg gattagaatg
    10981 caagaaatgg cttaaaaatc tccaatcagg acaaatcaag ttagacaaag tttccgattt
    11041 aaagatattg attgaagctg atttaatgct aaaagatatt gaaaattaga aaacaaaact
    11101 caacctaaga agattgcgag gtggtgtgta ttgaatggca agacaacgtg atccaagacg
    11161 tgatgaagcc aaaagaattt ggttagaatc caacggagaa aagcagttaa aggaaattgc
    11221 atctgaatta aatgtttcag attctcaggt tagaaaatgg aaatcgcaag acaaatggag
    11281 cgctgaattg aaaagtaacg ttaccaatgg caaaagtaac gttactaatc aaggtggcgc
    11341 tcctattggt aatcaaaatg ctaaaggtaa caaaggaaac agcagagcat caccgccaaa
    11401 gagaaataag aatgctctta aaactggtga gtatgaaaca atattttttg acacgttaac
    11461 cgataatgag aaggacatct attctagttt gaatgatgat ccttcttttg ctttgtccga
    11521 agaaatacga cttcttaaaa taagacaact tcgaatgatg aaaagaatcc aacaagctga
    11581 agctggacta aatgatgaag aagtcgaacg attgcagcag ctaagaaaga ttaaaactcc
    11641 gattgaaaaa aatggtaaaa agctagaaat caagcgtgag gttatgcaag atgtgcagat
    11701 tagcagaaaa aaacatcgca aaattgatga tattctttca attgaagatt cattgactcg
    11761 gattagcaac cagttagcta aagccatcaa gcaaatgaat gaactttata tgaatgaata
    11821 cagaactgat ttaattaaag ctcagactga taagatccaa gctgagacaa atgaaattgg
    11881 cggaaataat tcaggtgaag aaatagaaga atggaaacag gcagttttaa atgccgcaaa
    11941 caaacgggcg gtgaaagaaa atgaataatg aatttattcc ttttgctgat attggttctg
    12001 ccattgatta ttactatgat aaaccagtag ctttttgcca agatattttg catttgaatc
    12061 ctgatgaatg gcaagaaaat gttttaaatg atttagctga attttcaaag gtttctgttc
    12121 gttctggtca aggagttgga aaaacagcat tagaagcagg agcaatactt tggttcttaa
    12181 cgtgtcgacc ctacgctaaa gtaatagcaa cagctccgac aatgaagcaa ctttacgatg
    12241 tactttgggc agaggtagct aaatggttaa atgatagctt gatcaaaaac ttactgaagt
    12301 ggacaaagac caaaatttat atggttggtg attcagagcg ttggtttgct acggctagaa
    12361 cagcgactaa accagaaaat atgcaaggtt ttcacgagga ccatatgttg attgtggtag
    12421 atgaagcttc tggtgtgtct gatccaatta tggaagctat tcttggtacg ctatcaggtt
    12481 ttgataataa gctgttgatg tgtggaaacc ctaataatat tgaaggtgtc ttttacgatt
    12541 cccacaattc agaccgtgat aaatacagag ttcataaagt atcaagctat gatagtaaac
    12601 gtacaaacaa agacaatata gaaatgattc ttaaaaaata tggaaaagaa agtgatgttg
    12661 ctcgtgtccg tatttttgga gaatttccca aaggtgcgtt ggattcattt atcagtcttg
    12721 aaacggttga attggctaca gaaaaacaaa ttagtgattc tttagtcaat aaaacaacgg
    12781 ttgctcatat tggtgttgac gtagctcgat atggtgatga ttctacgatt ctctttccta
    12841 gaattgctac cagggcattg gagtatgaga agtattcaaa acgtagcact atggaaacaa
    12901 caggatatgt catcaacatg gccaagaatc taatgagtca atatccgagt attgataaag
    12961 tgatgattaa agtcgatgac actggtgtcg gaggtggtgt aaccgaccgc ctagaagaac
    13021 ttatagaaga caagcattat ccttttgagg tgtttggagt gaataacggt tcaacatcag
    13081 aagacgattt ttacgataat ttaggtactc aactatgggg aaacatcaag gaaatgttag
    13141 aagaaaatat gacagcaaat cttaacggag aacagcctgt tattgaattg ccttctgata
    13201 gttcgttaat caaagaatta agtactcgca aattcaaaat gacaagtaga agtcgtatac
    13261 gtttagaaag taaagatgat atgaaaaagc gaaatattgg tagtcccgat attgctgacg
    13321 cactggcttt agcgttttat gagccaccaa gtcactatca atttattcaa ttttaggagg
    13381 tgagtttttg attagtacag tattatcttt agagagatat aagaagctac gtgttaaata
    13441 tgcaacacaa attgaagatg gaatgtttga tccgaatggt tttatagaat atatgaaacc
    13501 attttttgct gatcgcgaaa gaaaatatct agcttatact agcgaaaaga atgaaataga
    13561 caatagacca aagcctaaca ctgatattgt aaaagttaat aataaacttc atgctggtat
    13621 gtattcaatt gtcgtagacc aagcagtcaa tcattttact ggtataccta tcaaatggga
    13681 ttacgatgta tcggaacaaa aaagaacact cattcaaaga ttaaaagata aattcttaaa
    13741 aaatgatata gaacttccaa cagttccaga agcttttaac aaattaacaa gcaatcttga
    13801 ttccatgaga ttcgcaatgc ttgattctga aactgcaact tttcaaggag cttgtggagt
    13861 agctttccga ttgttagagc ctgtggaaga agatgatggt tggaagttaa gagcaagtaa
    13921 tattgaacct tggagagcag aaagatatgg aaatgccgga atctatatca aagaaaagta
    13981 tgactcttac cagaaaaaat tttttcaaga aatgaaagtt atgacaagaa ataaaattct
    14041 gacatatgct tgctatggtg atttgaattt tgttacaagc ggaacgttca aaaaaattga
    14101 tgagaccgat aaccctttgg ggacgattac cttggcagaa tttaaaaata atacgaatcg
    14161 ttattgtgat tttgaagtag ctgaggaaat tggagatgca attgatcgtg ctttgtctga
    14221 tcaacaaaac gaaatcgaac aatttaagct tgcgtacatg cttgttactg gcacaacaat
    14281 gagcaaaggc actgctaaag aaatgatgaa tcagctaggg attatcaact taaaagaccc
    14341 tactgcaaaa gctgaatatg tcacaaaaaa attagcaaaa gatttcaatg aatatcatat
    14401 ggatctattg aaaaaacagt tttacaccat ttgtaaagca atcgatttta acgatgaggt
    14461 gtttaaatca aacagttctg gagaagctcg caagtggcaa atcattagcc tagaagcgaa
    14521 aacaaatact aaagagcaat attttagaga aggattgaaa gaatgcgcag aaacaattgc
    14581 tgcttttctt aagtttcatg acaaagtaga aattgagcct gaaaaaatta tttttacttt
    14641 ctctagatct ttgccaactg atttaagtta tttagctgaa gctttaccta aacttgcacc
    14701 atacgtatcc aaacgaacta ttcagagcca aattccattt gtaacagatg ttgattatga
    14761 aaatgaaatg atggaattgg aaagtggaag tgcttatcca gatagtgaat acaattttgg
    14821 cggaggtggc aatagtgacg atagaaacga agtattggac caaacgtcga gaactggagg
    14881 atcaagcaag gctcaaacaa gaaaatcaga cacttaaaaa attaactagt gtatttcctg
    14941 aagcactgaa agagatacaa gcaaaactat tatcacaagc tgacttacac aatatcactt
    15001 atccagaaat gatggagttt tacagtacaa gtaatcagaa aaaatatcgt gaatatgtgg
    15061 aaaaaaatta taagtcatta aaaatgtatg atgcaaaata caaagagttt atcgatgaat
    15121 tttttccacc atttgactat gcaaaagtca atcgcttatt acaaatacga tcagatgtat
    15181 ttaaaattct tgcagaatat gcgatggatg cagatgtgaa tcaatatttt tctgatcgct
    15241 tagaggaaat tcttcaaaga acatattctt ctaatgctaa tgtttttgtt caacttttaa
    15301 acgttgatat accaaattat ttaccagaaa atgaaattaa acattatttg aactatccat
    15361 ggtcaggaaa gacattttca agaagacttt ggggaaatat ttcatcgctt gaacagaaac
    15421 tctctaatgc tattgtaaaa agtgttgcta gtggagaagg cgttatacac gcattaaaca
    15481 ccatgagatt agactcagaa atttgtgaca tgtttaagtt agaagaatca aagtataaca
    15541 aagcgataga aaatctcgtt cgaacggagt atgcaaaatt tgcacaagat ggtattgaaa
    15601 aatcatattt agaaacaggt attgaggaat acaacgtatt gactgcaaaa gatgagagag
    15661 tttgccggat ttgtggagga aaggcaagta agaatcccta taaactgaaa gatgctgtca
    15721 taggtgaaaa tcgagcacct ttccatagtc gttgtagatg tacggatgtt cctaatttac
    15781 caaaattagg aaaggatatt gatgaagaat atgaccgttt atttggcgat ttattagatg
    15841 agtttgcaca tgattctttt ggaattaatt tgaaacggag gaagtagaat gaaagatttt
    15901 tttgaagcag tactaacaat taatgtaaat gctgatattg cagaagccta caaaacagct
    15961 attgagtctg agaaccatcc taatggcttg agagaccatt ggaatggcaa ttatgcctac
    16021 gtggttattg gcgatcaaac tgttaattat caagataata ctccagttga taagaatacc
    16081 gttaatttaa cgattcaatt attatctcat tcattaccaa atttaaaaga aacagttgat
    16141 tggtatgaaa agatgggatg tattgttgtt agaactgact acaaagaagg aaagtctagt
    16201 aattaggctt ttttattttg tccgaaatga cgttaaacta gcgcaatact gggcttggtt
    16261 gaatggtggg gcgcaactat taaaactcaa agcaatgcgg ggcgtgaaaa cgaatcgtgg
    16321 ggcgaaagga gaatgagcat gaaacacaaa tcattaatgc caatgaattt gcaatacttt
    16381 gccgaaggtg acgatcaaaa gttttctttt gatgacttca aatcttttgt ggaatccaat
    16441 gaagaagctc aaaaatttat tcagtcacaa tctcaaagtg ttgccgacaa acaattggaa
    16501 gcttggaaac aaaataactt agataaaatt aaacaggata ccatcaagga atatgaggaa
    16561 tctaagaaaa acaagtcacc tgagcaaatt caactggaaa aactacaagc tgaatttgaa
    16621 gcagaaaaag cgttgcgtgt gacaagtgat aataaagcat ttgttgcaga acaaattgct
    16681 ggattagaac tagatggaga gttaaaagag tctatttctc aatttatgct aaataatctt
    16741 gttagttcgg atacagattt cactaagaat gctgttgaag gttttacaag tgttttgaat
    16801 gcaattaatg agaaacatgc agatgcatta aaagaactac aaatgaagtc tgcatttggt
    16861 ggaactcaac aatcgaataa ccaggttcag cagaacaatg aaacattaac aaatccagaa
    16921 gaacaattag gacaaattct tcaacaattt aactaggaga gtgaaaaatt atgaaaaaaa
    16981 catctttaaa taatttagag tatttggata tttcaccagc gattaatgct atgcaagtac
    17041 caaatacacc ttttttaagc tatttacttg gtgctggaaa aacagagcca gcaaactcga
    17101 cagaaattaa atggcgtgaa tatgatatca acaacgatga ttcttctgaa aaacttgagg
    17161 gcggagaata tccagatgct gaatcaggtc gaacttggtt taacaactat actgaaattt
    17221 ttagaaaatc aacctctgta tctggtacat tagatgctat taatgtgaat ggagtcggaa
    17281 atgaattaac taatcaagta gcactacgtg gtatggaaat gaaaattgat ttgaaccgaa
    17341 aattgattac tggtgtaaaa gctgatgaaa atggttctaa aggtcgtcga atgaatggga
    17401 ttttgaactt gatcaattca gcaaataagg cagaaacagc cactgcgggt gcagtaacaa
    17461 gaaaagatat cgatacttta tttaaattga tgtatgaaaa aggttatatg ggagaaaaac
    17521 tatgcttgat ttctccagat atgcaggagt taatgactga tgagttagat ggaaaatcaa
    17581 caaaaattgt tcagttcggg gaaagagtaa cttttggatt gcaaattgga aatatcgtgt
    17641 ctaattacgg tacaggtatt gctctactag aaccatcatt gccaaaagga acaattgccg
    17701 caatcgatac taattatgtg aaactacgtc cattacgtga atggagagca gaagaacttg
    17761 caaaaacaac ggattctaga cgtatcggtc ttgtaggtga atactctctt gaatacaatg
    17821 cttcaaactc tggggcaatt ttaaatttaa agtctgaata aaagggagtt agtactccct
    17881 tttttgatag gaggaattac agtggtaaaa aaagatgaaa ctaaaaaaga tgaagtcgtg
    17941 aaatatagag taggtaaaac taaaaatttt gttgggtttg ttcatcctaa aactcgtaga
    18001 tttatcacag cagattcaaa taacgaattt atcatttcta tagatgataa agaagcaatt
    18061 gcaattttgg aagatgcaat tgatgttaat gaaatttagg aagtgatctg atggatgaat
    18121 cgctaaaaac ggaaatcatt gagtctacaa aagaagattt tccagatttg agtgaagaac
    18181 gcataactaa tttattagaa ataattttgc tagaaattga atcatacaac acttgtaaaa
    18241 atgatatttc atgggaaaag ttaaaaagcg tgattagtga agtgttgtat aaaataataa
    18301 aaaatgaatc agaaaaaaca gtatcttcag ttagacgtgg cgatacaacg attagttatg
    18361 cttcaacaac aaatgatgtt agtgagttac ttctaggcta tggtgatttg atacgaagag
    18421 ttattggttg tggaggattg gagttttttt aatgaatgaa gcagatattt tagaaactac
    18481 ttatgaagat agttgtatta ttgaaagact gacggacatt gaagattcta atacaaatat
    18541 tactattcaa gattacaaaa aagtatatga taatcctatt tcttgcgctc tttcacaagg
    18601 tcaaatcgat ggactagcag tcatagaaga tggagagatg gtaaatgttt caactgacac
    18661 atataaatta tttgttcatc ctaagattaa actaaaaaaa ggagatcgaa taacaataac
    18721 tcaaaaggcc agtggcttaa ttttttctct atttgctact aagccttttt actatcctag
    18781 tcattgtgaa gtgaatttga taggaagtga gaaaaatggg taatctcaaa tatgaatcaa
    18841 atgcagaaaa gatcattgag aattttaaaa atatgactgt aattgctcaa aaagaaggaa
    18901 tatccttcgt taacgattca atgaataaag tcgttagttt agctaaacca ttaactcctg
    18961 taaaatcagg taatttaagg cgaggatatc gtgtagtaaa agctagaaaa ctatcaagcg
    19021 gtcgaattgt tggagcagtt ataaacaatg aacattattt taaatatgta gaagaaggtc
    19081 ataggactaa aaatggtggc tttgtcaaag ggaaattcat gttaactcgt gcaacgaatc
    19141 ttgcaaatat gtcttatatt cctcgaagat ttaaacaaat ggcaataaaa atcgttaaga
    19201 aaggaaagta acatgaaaga tgaaatcatt gctgcaatca gtagcaaatt aaaagaaatc
    19261 tatcctgatg ggacaatata tcttgattca gttatgcagt caactaaaga tttttatttc
    19321 gttttatcag taatggaatc tggaactgaa aatgtaggaa ttgatattca aaatgtttct
    19381 ttcttaattg atattgcatt gattgataat aaacctaata gaaatttaat taatgaatta
    19441 gtctcacgtt gtgggacttt ttttaatacg attacaatcg atgagcaaat attatttcca
    19501 aaagcctatt tacctgatga agcagatggt gttcaacata ttcgttttac attagaattt
    19561 ccacaatata ttgaatggag tgaaagataa atgggagaaa aaagaagtaa aaccggaatt
    19621 atttctgtcg aaaaacctac ttggtttcct ttggaagatg agacagggaa ttttccggta
    19681 tatggtactg cgttgccaat gggaacagca gtaagtatca aaccaacagc taattatgaa
    19741 acaacacaag attatgggga ttcagtggtt caagatcaat ttacagcttt tggtggtgca
    19801 gaagttgaac tggaagcaaa tggatatact cataaagttt tatctacaat tactggaggg
    19861 aaaattgtta aaggtggtgc cttgcgttct ggagaagata ttgcgcaaga tggtgcgttt
    19921 gcatatagac gtaaaaaatc gaatgggaaa taccgttata cggtctttta taaaggacag
    19981 tttgctctag attcagatga aacatcaacg attgaaggaa gtaaagtaag ttttactcat
    20041 ccaacttgga agggttcatt tgtagatgta ccaggacttg gatatatgta ttcagttgat
    20101 gaagatgatg aaggcgtgga taaagcaatg atcggaaatt ggtttacaaa agtagcaatt
    20161 ccaattgaag aagcagaact ttcaggaggt acagaataat gtcaaaatat caaacaacga
    20221 ttaaattaac gaaaaaagat gaagaaggta aatatgagca agtacaattt aaatctgctg
    20281 aatttctacc aggcccagtc gtagaagaag cagcagaagt aatggaggga atgcaaaatg
    20341 ctgtagatgg aaaatcagta tcagaagcat taagtcgagc ttattcattt attgcagata
    20401 ttttgtttga aggacagttt actggtgaag attatcgtca agggatcgat gctcgagaaa
    20461 ttgcttcgtt gacaggtaag ttattaaaat ctgttactgc aggctttgat gaaacttata
    20521 cagaaacgaa aaaaaagtaa gcgaagctct caaatatcct ttttttaaac attcgattac
    20581 ttatcgagaa ttagatttaa agacgcaatt actcgaagca ggttggacgt taccagaaat
    20641 tgaacgtact gactttgacg agttaatgcg tctttttgct tttcgagatg cagtaaaaga
    20701 gcatgaggat gttgagtact acgataactt cacccaattt taggaggtga tactttgaat
    20761 aacgatgacc taattctgaa gatgatacta gatgaatctg gttttactgc cggtatgaat
    20821 aatgctgtca aaaagttgaa ctcttttgat gaaacaatcg aaagaaaaag tagaaatagt
    20881 ggcagttcat taggtagtat ttggaaaata tttgctggaa gttttctagc tagcggagta
    20941 actagaatcg taggagctgg ctttgatcta atcaagggtt ccataagtgg agcggttgat
    21001 cgagtagata cgatgaataa cgctctacga aatttccaaa atatgggatt tagtaattca
    21061 gaaattatga agaatatcgg aaagaatggg cttttatctc aaggtattca aggacttcct
    21121 actgctttga atgatgcgat aagtcatgtt caacttcttg cttcttctac aggtgattta
    21181 actcgttcaa ctcaaatatt taaagcttta aatgatggaa ttcttggttt cggtggttca
    21241 actgatcaag ttaatgaagc tgttattcaa ttatctcaaa gtttctcaaa cggaaaagta
    21301 gacgcacaaa cttggaactc aatgatcaac gctcaacttg gacctactct ttctgctatt
    21361 gctaaaaaga tgggaattac aatgggagat ctgaaagaag gtttatctca aggtaagatt
    21421 tctgttgaag aattccaaaa tcaattaata gaaatggata ccaaaggtgg cggaggactt
    21481 aaatcattaa gtcaaatcgc taaagattct actaagggaa ttaaaacctc tatacaaaat
    21541 gcaaaaacag ctgttacacg tggtgttggt gaggtaatag aaggattaaa taaagcatta
    21601 gtagattcag atttaggtgg atttaaggga attattgata aagtgggcag ttcaatggaa
    21661 tcgttcttaa aagtaattgc tgcaaatatt cctacagcag tatcattttt aagtaattta
    21721 tttaacgaaa ttcaaaaatt tggttctgct ttgaaattca tgatgccctt tcttgttcct
    21781 gcagctaccg cttttggagc gttcatgttt caacttaaag gtataccagc aataataaaa
    21841 agcttcaata attttaagaa tgccataatt ggcgttggaa attcattaaa gattatgggg
    21901 gcaatagctg ctgctaattc atttgtttta attattggtg cagtcgttgg tgcaatagct
    21961 gttttcggtt actttatggc aaccaatgaa gagcttagaa acaaggttat atctatttgg
    22021 aatgatgtga aagattccgt aattggtgta ttaaaaaata taaaggactg gggaattgat
    22081 acttggaatt ctgctaaata aatggcatca aatgcagttg agggtgtcaa agatgcttgg
    22141 tcaggaacaa aggaatggtt ttctaacacc tggaaaggta ttaaggatgg ggccacaggt
    22201 ttatttgata aaacagtaga gacttcaaga aatgcagtcg atagtgtaaa aaatgcatgg
    22261 tctaatatga agaaatggtt ttctgatact tggcaaagta taaaagattc agccagagaa
    22321 aaatggaatg gaatcaaagg ttctattatg tcagttgctg gtccattgat tacaggtata
    22381 aagaatgcat ttttacatgt tactttttat ctagagacct tatggaataa tttagttgag
    22441 attggtaaaa acgtctttga gattttaaaa aatgttatac ttgcgccggt tttgtttatt
    22501 acctccatga tttctggtgg atggga
//
LOCUS       DASJSZ010000048        22471 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.048, whole genome shotgun sequence.
ACCESSION   DASJSZ010000048 DASJSZ010000000
VERSION     DASJSZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.048"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            44..1185
                     /locus_tag="U4B03_001665"
                     /pseudo
     CDS             44..1185
                     /locus_tag="U4B03_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1311..2390)
                     /locus_tag="U4B03_001666"
     CDS             complement(1311..2390)
                     /locus_tag="U4B03_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2068519.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2493..2933)
                     /locus_tag="U4B03_001667"
     CDS             complement(2493..2933)
                     /locus_tag="U4B03_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2068520.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2990..3400)
                     /locus_tag="U4B03_001668"
     CDS             complement(2990..3400)
                     /locus_tag="U4B03_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN2068521.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3580..4014)
                     /locus_tag="U4B03_001669"
     CDS             complement(3580..4014)
                     /locus_tag="U4B03_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2068522.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4114..4962)
                     /locus_tag="U4B03_001670"
     CDS             complement(4114..4962)
                     /locus_tag="U4B03_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2068523.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5351..7117
                     /locus_tag="U4B03_001671"
     CDS             5351..7117
                     /locus_tag="U4B03_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEN2068524.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7219..8478
                     /locus_tag="U4B03_001672"
     CDS             7219..8478
                     /locus_tag="U4B03_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2068525.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8625..9926
                     /locus_tag="U4B03_001673"
     CDS             8625..9926
                     /locus_tag="U4B03_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2068526.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9929..10780
                     /locus_tag="U4B03_001674"
     CDS             9929..10780
                     /locus_tag="U4B03_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2068527.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10793..11608
                     /locus_tag="U4B03_001675"
     CDS             10793..11608
                     /locus_tag="U4B03_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEN2068528.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11759..12925
                     /locus_tag="U4B03_001676"
     CDS             11759..12925
                     /locus_tag="U4B03_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2068529.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13155..13802
                     /locus_tag="U4B03_001677"
     CDS             13155..13802
                     /locus_tag="U4B03_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN2068530.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13886..14830
                     /locus_tag="U4B03_001678"
     CDS             13886..14830
                     /locus_tag="U4B03_001678"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEN2068531.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14965..16416
                     /locus_tag="U4B03_001679"
     CDS             14965..16416
                     /locus_tag="U4B03_001679"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN2068532.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16437..17273
                     /locus_tag="U4B03_001680"
     CDS             16437..17273
                     /locus_tag="U4B03_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN2068533.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17273..17932
                     /locus_tag="U4B03_001681"
     CDS             17273..17932
                     /locus_tag="U4B03_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2068534.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18058..18747
                     /locus_tag="U4B03_001682"
     CDS             18058..18747
                     /locus_tag="U4B03_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068535.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18753..19709
                     /locus_tag="U4B03_001683"
     CDS             18753..19709
                     /locus_tag="U4B03_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEN2068536.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19753..20265
                     /locus_tag="U4B03_001684"
     CDS             19753..20265
                     /locus_tag="U4B03_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2068537.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20521..21069)
                     /locus_tag="U4B03_001685"
     CDS             complement(20521..21069)
                     /locus_tag="U4B03_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2068538.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21158..22339)
                     /locus_tag="U4B03_001686"
     CDS             complement(21158..22339)
                     /locus_tag="U4B03_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEN2068539.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         7576 a         4070 c         4322 g         6503 t
ORIGIN      
        1 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
       61 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
      121 agaggagaca gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
      181 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
      241 agcgaccatc aaagcaaaaa ttgactattc gggagactta gcacctatac tacaatcagc
      301 tgttacattt gagaatgttg atcccacttt gtcgattggc tatgacgaga acactttgac
      361 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
      421 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atttaacgcc
      481 gtatagtcag cggcagacaa ttgagcaaga ttcagccatc gactcaggag gagatcttgt
      541 tgaagaacca acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa
      601 tccagacgga gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca
      661 accagagcca gacaataaat caaaacctga aataacccct ggagaaaaac cagatgtaac
      721 tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga
      781 taatgaaact gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac
      841 tccagaacca gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac
      901 aattcaagag gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa
      961 tgaagagcag ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt
     1021 aaaaagtagc aaggatacaa cctttgctgt cctgaaacga aaaataaaca gctaccaaaa
     1081 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
     1141 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
     1201 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa ataccagatt
     1261 gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt ttaagggata
     1321 tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag
     1381 gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt
     1441 aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc
     1501 agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct
     1561 cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct
     1621 gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag
     1681 ccaaatacag ccaaggcgat tgtagtgagc aacgtattta aaagagcgac aacaaattga
     1741 gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga
     1801 aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt
     1861 tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct
     1921 atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg
     1981 tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc
     2041 tcgtaaaaat taataacgga ctggatcatg tcaaatgttt gatgaataag catcgggata
     2101 tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa
     2161 acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa
     2221 gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa
     2281 acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa
     2341 acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg
     2401 aatatgaaaa taagaaagag caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg
     2461 gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc ttgatgaaaa
     2521 ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg ttcaagtgga
     2581 agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc agcaccttga
     2641 gcagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg attaatataa
     2701 tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag acacaaatca
     2761 tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat cacaagatac
     2821 tgggtatagg taagattcag cggcttgagg aggggagtgt agtgtctaat gatttcttta
     2881 gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc catttttcca
     2941 cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc tattcactaa
     3001 ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga cacactttat
     3061 gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata gatccttcaa
     3121 tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact tgtgcactga
     3181 cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca ctgttaaaac
     3241 atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg acgcgtttac
     3301 caggagctgc aatattcaat tcgaatgtac gatcgggatt atgcacttct ccagagcgtc
     3361 cgcctgtgtt gatcattttc gtagaataca tttttttcat aaagttacca cctttaatta
     3421 tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa tggattggca
     3481 taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc aaacatatac
     3541 aaaaaatatc agagacaaaa aataatctca tcataaacat tacatatatt tctcaaaaag
     3601 ttgttttctt tcttcaaaaa ttacttcacg atggacctga tgaagcatcc ataaataacc
     3661 aaaaggatct ctgaacattg agttagtcac accaaagtcc atcatttctg tcatttcttg
     3721 aatctctaca cagccatttc gtaatgcttt attatgtgtg tcctcgatat cagagacgat
     3781 gatattgaac cagatcgtat ttggatgttc agcagtagga gcagtcaaac caagagacgt
     3841 gttttcatcc agcatatgga atctcgtacc atacaaggta aaaatgactt cattattgcc
     3901 tctagggtaa tctgttgcct caatcagttg tatttcatca aagattgttt gatacagttc
     3961 tagtgctttc aaactatcat ttaatacaaa atcgatttcc gttcctttat gcataaagat
     4021 ctcctctcct tttcagtcga acagttacta gattctttta ttataccagc taaagaaaac
     4081 gcattcaaaa aaatagcgga tttacttacc aactcattcg attggatgat aagtaaatcc
     4141 gcgtcccatt acttctgcta cgctgctgac aatgacaaag gcacttggat cgatttcttc
     4201 tatcaatggt ttcattcggg aaaattcatg atcagtcaca acgatcaata aaacctcttt
     4261 tgactttcga ttatacccgc cttttgcctc tagcagggta aggccacgtc caatctcagc
     4321 acttaagcga aggcggatgt cttccaaata atgttcactc atgaccataa cagatttctt
     4381 tcgattgata cctgtttcga tataagtcat gacaattgaa gtgattgcaa tagataaaat
     4441 agcataaaga aactcttcta ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc
     4501 tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat tttttaaaaa tgaggggagg
     4561 gatcgtcgta ccgccgctag aagagttgtt tttatataaa atcgcaactc ctaaagcaaa
     4621 aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg tttctggaac
     4681 tattgcaatg attaaaggaa aaaccaagct tccgaaaagt actttaaaaa agggtttttt
     4741 acctaaaaat agaagagcta gtaccaacat aacgatattc aacactaaaa taactatagc
     4801 tcggtcaaag cctaaagcag cttccagtaa tattcctaga ccgctgacac ctccagcagc
     4861 aatatgatga ggccctaaaa acatattgat agcgactcct aatatgaaga gggcaaaaat
     4921 aatcttaatg tatgtccata taaattgtct cgtttctttc atttattcac ttccttaatt
     4981 cattaataat actcttatct tattataact tacttttcac cttaagttaa ctttaattaa
     5041 taaaaaaatt aggctagtct aaattattat gtttcattct tctagaatat acttctacgc
     5101 caaaaactgt taaaaaactt ttactttaga aaattggaga aattgatcgc ttaagataaa
     5161 ttcaggacac taaatagtac aggaagtaga ctaaaacttt taaaagtttt aagtctatct
     5221 atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga taacattttt
     5281 gaaagcgttg tcttttgctt ctctatcact tatagtgatg acgtcatatt aaaacacaag
     5341 gaggaaatca atggatacag ctgcaattta tcatcgaccg gaaagtgaat atgcatttct
     5401 atatacgaat acaagatttc gtattcggct tcgtactagg aaaaatgata tcaaaaaagt
     5461 atatgtgcta tgtggagatc catataccat aacaactgaa aaatggtatc aaaaacaacg
     5521 accaatgaaa aaatgtctaa gtaccaatgt tcatgattat tgggaaattg aagtgacgag
     5581 cgaaacaagt cgcttacagt atgcgttcca tgttgttggg gaagataaca cggactgttt
     5641 ttacggtgat caaggaattt ttccttatcg agaaaatgtg attactgaac caaattttta
     5701 ttttcgaata ccttattttc atcaaatcga tcgattcact attccagaat gggtaaaaga
     5761 aacagtatgg tatcaaattt ttcccgaaag atttgcaaac ggggacaaag caaatgaccc
     5821 cgaacacact ttaccttggg gaagtaaaga tccagaccga gaagactttt tcgggggaga
     5881 tttgcaaggt gtgatggatc atttggatta cttgacggac cttggcatca atggcatcta
     5941 tttttgtccc atatttaaag ccacatccaa tcataaatac gatacgattg attattatga
     6001 ggtggatccc gcttttggcg ataaaaaact tttaaaaaac ttgattgatg aagcacataa
     6061 aagagggata cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc attcaccaca
     6121 gtggcaagac gttctcaaaa acggagaaaa atcgatctat aaagactggt tccacatccg
     6181 ttttttccca gtagattctt atcagatgac tgacttacca gaaactgctg aaaatatccc
     6241 atatgatact tttgctttta ctccattcat gccgaaacta aatacagcaa atccagaagt
     6301 gcaaaaatat ttgctggata tcgcaactta ttggataaaa gaattcgata ttgatggatg
     6361 gcgtcttgat gttgctaatg aagtcgatca tcatttttgg aaaaaattca gagaagcagt
     6421 tacagaaatc aaaccagaca tctatatcct tggagagatt tggcattcct ctcaagcttg
     6481 gctgcaaggg gatgaattcc atgcagtgat gaattatgca tttacagatt cgattaaaga
     6541 ttattttgca aaaaagaaaa tcacagcttc acaaatggtc agtggaatga atcatcagca
     6601 aatgctttac agagatcagg tgaacgaagg aacattcaac ttattagatt ctcatgatac
     6661 tgccaggatc ttgacccttt gccaaggtaa caaagaattg atgaagtctg tgatggcctt
     6721 tatgtttctt caaaaagggt ctccttgtat ctattacggg acagaaatag ggatgactgg
     6781 tgaagatgac ccggattgcc gcaaatgtat gatatgggag aaggaggaac aagatctaga
     6841 gctattcggt tttataaaag aacttgtttc tctaaggaaa cagttgtcta agattatttc
     6901 tgaaggttct actcagtggc taatagtaaa tgatcgtgaa gataagctct atttcacacg
     6961 gaaactagag ggacagatca tctatgttta ctttaatcag tcaaaagaac cttgggtagt
     7021 agaacaggaa aatgaggtga ttcttagtca aaattgtcag ttgttagaag acgggaaagc
     7081 agagatacga caatacggat ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag
     7141 aaagcgtttg caaatgaaga tttgtgccca ttataataag ggtgtaacag cacgttgaat
     7201 acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt agggttaatc
     7261 tctttaggaa tcttaggcgg attagcggct tgtggcaatg gtaactcttc agcagaaggg
     7321 gaagccgatg aaggaaaaac gttaacagtt tccgttgatt ctggatataa agattatata
     7381 aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag
     7441 gacatgttcg atcagttgga atcattagcg ttagatgggc ctgctggaaa gggacctgac
     7501 gttatgatgg cagcatatga ccggatcggt gcgttaggac agcaaggaca tctggcggaa
     7561 gtaaaactgg gaaatgaagc agcttacgat gaaacagaca aagcccaagt gacttatgat
     7621 ggaaaaatct acggagaacc tgcagtaatc gagacccttg ttttgtacta taacaaagat
     7681 ttagtcgata cagcaccatc aacgtttaaa gatttggaga atctatccaa agacagtcgt
     7741 tttgcttttg agtcagaagc aggaaaaaat acaggatttt tagctaaatg gactgacttt
     7801 tattattcct atggtttaat tgcaggttac ggtggctatg tatttggtga tgatggcaca
     7861 aatccttcag atatcggttt gaataatgca ggtgctgtag aaggaatctc ttatgcaaca
     7921 gattggttca aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc aggagacttc
     7981 gccagccagc aattcatgtc caataaaact gcagcaatca ttgacggtcc atggcaagcg
     8041 caaacataca aagaaaacaa tatcaattat ggtgtagcca aaattcctac tctaaataat
     8101 ggacagcctt atcaaccgtt tggcggagga aaaggatggg tagtcagcaa ttatgctaaa
     8161 aacaaagacc tctctcaaaa atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa
     8221 ttctttgaaa tggtaaatga aatccctgca aaccaacaag ctagggaaac agcaaaggct
     8281 aaaaacgatg aattaacaac agctgtgatt gaacaatatg aaacagctca agcgatgcca
     8341 aacatcccag aaatgggcga agtttggaca ggtgcagaaa acttgatgtt cgatgcagca
     8401 tcaggttcaa aaacaccaaa agaatccgca gatgaagcag taaaaacaat cagtgaagcc
     8461 atagaacaaa aatactgatt aaaatgataa ccgtccattt ttataaaaaa tggaacaggt
     8521 gcttcagata aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt tgccattatc
     8581 ttaaaagcag tttgcggttt agcaagttta gagggggcat tcagatgcag caagataaaa
     8641 caagcaatat caacgaaaaa aaaatcacgc tttgttcact tattccaggg atggggcagt
     8701 ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc catattcgtc ctattcattg
     8761 gtcagatgct tctgggcgga tttcatgcat tggtaggatt ggttactttg ggaagtatac
     8821 caatggaaga tcattcatta tttattttga ttgaaggcac tttgcaattc atcattacaa
     8881 tcattttctt gattttttac ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc
     8941 gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt catacataat ataggtgatg
     9001 aaggttttcc ttatctattg acacttcccg cttatcttct catgattttt acgatcattt
     9061 tcccagtcct agttacattg ttcactgcat ttactaatta tgacttcaat catattccac
     9121 cagccagttt aattgattgg gtaggggcta aaaacttttt tagtatcttc tttttaagct
     9181 catatcgtaa tacttttttt gcagtctttt cgtggaccgt gatttggact ttatgtgcaa
     9241 caactttgca aatcaccctt ggtattctca cagcggttgt tgctcaccag aaatttatta
     9301 aattcaaacg tattttcggt atcattttct tattaccatg ggcggtccca gcttttatta
     9361 ctatcatgag tttttcgaat atattcaacg atagcgtagg tgccatcaat actcaagtga
     9421 ttcctttcat aaaccagctt ccttttgtag atatcagtgc aatctcttgg aaaacggacc
     9481 cgaactggac gaaagtagca attatcttag tgcagggctg gttaggattt ccctatatct
     9541 atgtgatggt tacaggaatc ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa
     9601 tcgacggagc aaatgcatgg cagcgctttt caaacatcac gcttcctgct gtcttcctag
     9661 tggcagcgcc aacatttatt acacagtata cggggaattt caataatttc tccatgatct
     9721 atctgtttaa caatggcggt cccggaagcg taggcggagg agctggttct actgatattc
     9781 ttatttcctg gatctataaa cttacgactg gaacttctcc gcagtattct atggcttcag
     9841 caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga
     9901 caaatgcgtt caatatgggg gattaacaat gaaaaaattt cattcgagtg ctcgattcca
     9961 aaaacgactt tctcactttt ttacttactt ttttctttta atactgtcag tcattatcat
    10021 ctatccgctt ttgatcacag caagtactgc cttcaagtca ggaaatattg cagcttttag
    10081 tttagatctt tcaagtgact ggactttagg aaactttact cggctattca gtgaaacact
    10141 ttacgggact tggtataaaa atacattgat catttctgtc ctaacaatga tcgtacaagt
    10201 gactgtagta actttggcgg gctatactta tagtcgctat cgttttatcg gccgcaaatc
    10261 aagtctgatg ttctttttag tgattcagat ggtaccgact atggcagcat tgactgcctt
    10321 ttatgtcatg gctttcttat tagaagcgct cgataagtac tggttcttga caatgatcta
    10381 tataggcggt ggaattccaa tgaatacttg gctgatgaaa ggatactttg acactgtacc
    10441 aatagattta gatgaatctg ctaaacttga tggggcaggg catttcagga tttttttcca
    10501 gattgtatta ccgcttgtca aaccgatgct cgccgttcaa gcattatggg cttttatggg
    10561 accttttggg gatttcatgt tagctaagtt cctgcttcga acacctgaaa atcagactgt
    10621 tgctgtcggc ttacaaacat ttatttctga tgtgacaaac caaagagtct ctctttttgc
    10681 tgctggggca attttgattg cactaccgat ttgtctatta ttcttctttt tacaaaagaa
    10741 ctttgttacc ggcctgacat ctggcggaac aaaaggatag gagcagagta tcatgcaaac
    10801 atttccgtta aactattttt ccgcattacg tacgccaact caagtgtttg cgggacgcaa
    10861 actattatca tggcctaaat ttttcttgat atttgtattt ttagtttcct tgatggtcat
    10921 gcctgtgact cttttttatg ccaatcagat ccaagcgatt ccattagaac aatttctatc
    10981 cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg aattatctga
    11041 aactgggtta caagcatcgc aacaaacgat agctgtgacc cctgaaattt tggtaggtgt
    11101 gtctttatct gagaaacaac aatccgatca tggaactttt attgattttg agaaggaaca
    11161 atgggtgatc caacaaaagg ataaatctgg tatacgccgc tatacgatga attatagccc
    11221 atctttccaa ccagattctg tacgtacgcc cgaagatttc caacgatttt tagaaagaga
    11281 attttatgcc agtaatcgtc caacaatcat tttatcttat agtttaagtc ttggtcttgt
    11341 cctatttgtc atgacgagtc tcattttatt cggggcttcc tttttcttgt ggatgacaag
    11401 aaaaagccaa ctttcatcca ttcatacatt caaagagagt gcaaatttga tgctgaatgt
    11461 tatgggaata ggttccatgg cagcagcagt tgtagggttt atccactttg actttatctt
    11521 gatgctaagt gtccaaacaa cgataactgt cttgctgctt ttatgggttt ttgtcaaaac
    11581 aagattcaaa gataagcgtg tcgaatgaca agtttaagcg atttatgcga caatgaatgt
    11641 gaaaaaaatg aaaaataaag tagtttttga aaaatttctg aaaaaattaa acttcttagt
    11701 tgattctttt cttttttagt attaacgtaa tagggtagtt tttttaagga ggaaaatcat
    11761 gttagagaaa actttataca atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt
    11821 aaattattgg gacggtacaa cgaaacaata cggagatacg gacactcctc cccaaatcaa
    11881 aatcaatatc catgaagaaa tcccaatgaa agagattaca agtaacgcat ctttggcact
    11941 aggagaagcc tatatggatc atcgaatcga gatcgaggga agtatccaag cattgatcaa
    12001 cgatgcttat cagcaagcaa acagcttttt acgaggaaaa gattatctaa aatggctgcc
    12061 aaagaaagaa aaacatacga aacaaaaaaa caaagaagat atccatgcgc attatgactt
    12121 aggtaacgac ttttataaac tttggctaga tccaactttg acttattctt gtgcttattt
    12181 caatacacct gaagatacgt tggaacaagc acaaatcaat aaagtccatc atatcttaga
    12241 caaactcttt atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt
    12301 gatgttcact gctaccaaag agtataatgt caaggcaact gggatcacac taagtgaaga
    12361 acaatacgat ttgattacca aaagaataaa agaagaacat ttagaagata aatgccgtgt
    12421 attattaatg gattaccgcg aactaaaagg cgaaacgttt gatcatatca caagtgtagg
    12481 tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt
    12541 attgaaacca aaaggaacag ctttgatcca tggaatcagc cgacaacaag gcggtgcgac
    12601 aaatgcttgg atcaataaat atattttccc tggcggttat gtccctggtg ttactgaatt
    12661 agttgataat attacaaaaa acgatttgca actgattgat ttggaaagct tacgtcgtga
    12721 ctaccaattg acattagaac attggacaaa gaattttcat caagtccaag atcaagtcac
    12781 gaaagaaatg ggtgattcct tctatcgaat gtgggatcta tatttacaag cttgtgcagc
    12841 ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa
    12901 tgacatccca atgcatcgtg cataattgag gcaaaagtac tgtttaaaga ctatttaaaa
    12961 agaagctaag ttttatgatg ctccagaagt gaactttttt gtctaacttc taggattgtc
    13021 tctaaagctt agtttctttt tctttatatt ttcataataa aagtttggtt ctttaatttt
    13081 taaacatgac agaaaatttt ttcttcgtta tacttgtaat agaatactat tttataacaa
    13141 aggggatcga atcaatgaaa aaaactgtta tttatactag tttatttgca gctttgtttt
    13201 tagctgcttg tcaatcaaat accaatcaag gcacatctgg gaatacgtct tccagtagcg
    13261 aacagcgctc ttcacaggta tcggactcct caacaaaagc aagcacatct tcgtctagcc
    13321 agacaaaaag ttctagtgaa gcaagtactc agtcagatat caaagtgtct gcagctcgtg
    13381 cgatcgaatt gttccaagat aaatacccag atactgcaat tactagtttg gaattagata
    13441 gtgattgggg atcttacttc tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag
    13501 caagaattga tgctcaaaat gaaaaaatgg agacagagaa tccagaaact ctagatcgag
    13561 atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga tgttgataaa ttgatcacga
    13621 ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag cggtacggca acagattggg
    13681 atttggataa agatttaggt actacttatt gggaagtcaa agtaaaagac ggcaatcaaa
    13741 gtacatcagt caagatcaat agccaaacag gcgaagtact gtcaactgaa caagatgatt
    13801 aagaatttct tcggaaaaca agagttgatt tttcttttag tcatgataca ctagacaaaa
    13861 taacttgttt gaaggagcga tttacttgag tttagaaacg acctttgcca atcatatctt
    13921 tgccaatcct ttgatgaatg catctggtgt ccactgcatg actacccagg aattagatga
    13981 actggctcat tcagaagcag gagcatttat cactaagagc tgtacgatca atgaacgaaa
    14041 aggaaatccc gaaccacgct attttgatgt tcctttaggg agtatcaatt ctatgggact
    14101 tccaaactta ggtttttctt attatctaga gtatgctctt gcgtatgaaa aagtgcagga
    14161 aaaccaaaat caaccgttat ttttttccat tgccgggatg agtgtccagg aaaacttgga
    14221 aatgcttgag aaaatagaaa aaagtgggtt taatgggatc actgaattaa atttatcttg
    14281 tcccaatgtg cctggaaaac cacaactagc ttacgatttt gaggccactt atgaaacact
    14341 gaaagaagta ttcagcatct tttctaagcc gctaggaatc aaattgcctc cttattttga
    14401 ttttgctcat tttgatcaga tggctgatat cttgaatcag tttccattga cctatgtcaa
    14461 tgctatcaac agtgtgggca atggtttgta tatcgataca gaacaagaag cagtagtcat
    14521 caaaccaaaa gaagggttcg gcggcattgg cggagagtat atcaagccga ctgctttggc
    14581 gaatgttcgc gctttttata cacgtttgaa accagaaatc caaatcattg gtacaggtgg
    14641 gattcggaca ggacaagatg catttgaaca tctgctatgt ggcgcaagca tgttacagat
    14701 tggtaccgag cttcataaag aagggccgga aattttttct agaatcataa aagaattaac
    14761 gcagattatg tcagaaaaag gatatacgtc aattgacgaa tttaaaggca aattacgcac
    14821 catttcatag caaagagacg aaggtcatga ttgatcttcg tcttttttta taaaaaacac
    14881 tcgtctgttc attcctgtgc aagcgtactc attgtatgtt agacgaactt gtgggaaaat
    14941 aacagaggca aaaaggagga taatatgaat gatgcaacag aattagcaaa agagcttaaa
    15001 gaaaaagaat ttagttttca agaactagtt acggatatac agaaaaaaat aaagaagcaa
    15061 aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg agagtaattc ccacgagcta
    15121 ttgattcccc agtcagaaaa agggccattg gctggtatcc catttccttt aaaaatgctc
    15181 ggtcaagaaa aaaagggatg gctagctact tcaggatctc gtttatttga aacacaccga
    15241 gcttctcata catctaatta tgtgcggcaa gcagaagcga taggtctagt tccttttgga
    15301 caaaccaatg cgccagagtt tggtttcaaa aatattacag acccagtcat ctatggccca
    15361 gcaagaaacc cttggaacct tgaatgtaca ccgggcggat caagtggagg agctgctgct
    15421 gcagtttcat caggaatcgt tcctttagct ggtgctagtg atggcggtgg ttctattaga
    15481 atacccgctt cgttttgcgg attgattggg ctgaaacctt cgcgagggac gatgccagtt
    15541 ggtccatatg cttggagagg atggcaagga gctgctattg atttcggctt gacagtttct
    15601 atgagagaca cggaggcttt attttatggg atgagaagta tccattccgg tgcgccatat
    15661 caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa aacggttgaa gatagctttt
    15721 tgtgttgatt caccgatcca ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa
    15781 gctgtagatt tccttggaaa agaagggcat gatttgacag agattcctta cccactagat
    15841 ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg cagaaacagc agcaatgatc
    15901 cgctcgattg aaacaggcat ccagcgagct gtcagaaaag aagaacttga acccatttct
    15961 tggactttgt tccaatatgg tcagaagctc tctgccgcta cttacatcga ttctctgcat
    16021 gtatgggatg aagcagcaat tgtcatggaa aacctacatc aagaatacga tctattcctt
    16081 tcaccaacga ctgcatttac cgcccctaaa atcagcgaag atttgcaaag tgatgcgata
    16141 agagcacaga tggaacacat cagtgattgt tctgaagtag aaggtttaga cgttgtttct
    16201 tccatgtttg aggagagctt gaagatcaca ccttatacac agttggctaa tctgactgga
    16261 caaccagcaa tcagcttgcc tactcatttg acaaaagaaa acatgcctct aggaatccaa
    16321 tttatggctg ccagaggaag agaagatttg ctatttcaaa tcggctatct atttgaagca
    16381 aatcatcagt tccatttacc aaatccatgg aaatagcaga aacaaggaga agaagaatga
    16441 agaaaaaaag attgtttgct ctaataacta gtgccttttt tattttaagc gcttgcggta
    16501 cagctgccgc taatcaggaa gataacagct ggagtaatgt agaaaaaaag aaagaattag
    16561 tagtagcgac ttctggcaca ctgtttcccg catcttatta taatgatcaa aatgaattag
    16621 ttggttatga tgtagatttg gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct
    16681 ttaaagaata caacgtggat ggacaaatca cttctgtttc aaaaagagaa tcagattttg
    16741 ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa attctccctt tcagagactt
    16801 tcaaatattc atttgacagt atgatcgtac gaaaaaaaga tgattcagga atccactcac
    16861 ttgaagatct taaagggaaa aaagcagctg gcgaacccaa tactagctat atgcgtttag
    16921 cggaaaaaca cggagctaaa ttagtcacat atgacaatgc cacgaatgat cagtatttaa
    16981 gcgatgtagc aaatggtcga acagatgtta ttctaaatga ttattatctg cagaaaatgg
    17041 ccgtatctgc tttaccagat atccctgtaa aaatactgga gaacgtttat ttcaatgaaa
    17101 acgaaagtgg ttttttattg aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg
    17161 tattaaaaga gatgaaagaa gatgggacga caaagaaaat atctgaaaaa tattttcaaa
    17221 ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc ggtgagtgag tgatgtactt
    17281 tcctctaatt gatttttccc tcatgtggac ttcacttcct ttcgtactgc aaggcttggt
    17341 atacacatta gggattggct tcgtgtcgtt tcttttagga aatctggtag ggttgctttt
    17401 gacagtttta ggtcttttgg attggctgcc tctgaatgta tttattcgtt tttatttatc
    17461 gttctttaga ggcattcccg cactagtcct gctgtttctg ttatattttg ggttacctta
    17521 tcaattaagt gccttaacgg cttcagtcat ctgctttacg attaccagca gtgcatttat
    17581 tggtgaaatc tatcgaggat cactagcagg cgtttcttca ggtcagtggg acgctggttt
    17641 agcttctggt ttttctttct ttggggtatt gaaatatatc gttttgccgc aagcttttcg
    17701 aatctcagta ccggcactca gtaacgtagc tatggatcta gtgaaaggta cttcgttagc
    17761 agcaacaatc acagtgccag aaatatttca aaaagcaaag atcataggcg gtcggacatt
    17821 cgattatttt tctctctata ttttagttgc actgatttat tgggtcattt gtatcgggat
    17881 cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat cagtcaatct gaaaaataga
    17941 aatggtgcta gcgtggctgg caccatttct attttttgtt tcttgtatat tctgcctagt
    18001 attttaaaac catttcatgt aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg
    18061 aagaccccgc taaccatcga aatagaatcc tcgttatacc aatattgcat cgagcaaggc
    18121 ggcttggttg tagaagaagt cacgatgcct gatgaccaag gaattgtaga tacgcttagc
    18181 tgcttgacaa aatttgatgg aagccgagaa tggcgatgct atgagctgaa agtgacaaaa
    18241 gcagattttc gctctacagc caaactgtca tttgttggac attataatta ttttgtttta
    18301 ccaagtaaac tatatgaaga agtaaaaact gaaatcccta caggaatagg tgttttagtg
    18361 tatcgtccgt acacggttga agaggagatg cctgcctctg gtacatttct gattgcgaaa
    18421 aaagcagcaa agaaagagct tgctgtaccg gaagaagctt tgacaaaccg cttcatggct
    18481 tctttattca gggaagtaag aaaagcaaag caaatggagc gaggcgtaaa atattttcca
    18541 acagatcaat tgtacaaaga attgaaaaga cgttcagaaa atcaagatcc attttctaac
    18601 gaacgatatt atgaacggtt tttagaggaa atagctagtc aggcaatcaa tgagttgcaa
    18661 gaagaagtta tcgcgcttca gcatgattat gaatatttga ggcaacaaca gcagatccgt
    18721 cggttgccta cagagccatt ggagtgaaaa atatgtacta tccttattta agaggaaaac
    18781 aatttgacct attagcatta aaagaagcac tgtcgcgagg attgctctcc aataaaatcc
    18841 aaccagtgat cgaaccagta cgtgattccg ctacgcttaa aaatgtgatc gaattatttc
    18901 aaaaaaagca ccatcgaatc gctgtgatcc aaaatcccca agtaggacaa ttcaaattat
    18961 tcgatcagca tgtgcacagt tggacagtca aagaaaatag ctctgtcgtc cctgcacaga
    19021 tcttaacgcc agaaaatctc agtgaagtac tggattcacc tccagcgttt cttgttttcg
    19081 atggacagca ctacccaaaa gatacagagg tgtggaaaca gttggcagga gttgattcga
    19141 aatttttgat accagatact agtcgctttc gcatctggct gcccgaaaac aaaattgtga
    19201 tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta tgcagataaa acagatgact
    19261 tttttagtga tgactatctt tttttccacg cagacggata tattggattt tctgatttca
    19321 cgatcgaagg tagccgttat ttcgataaag ggttccctag ccgagcgctt gctatccatc
    19381 taacgtatat tgatgcttac ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg
    19441 actctgctaa agaccaagca ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga
    19501 tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat gattgaatta ttggctcttt
    19561 accagcaaca aaaattccct ggactgggag tgttgaaaaa gtggtcactt atgcatcatt
    19621 tggaacttat tagtcacctg ctagatcatc caaaagattg gcttaaatat tattcaaaag
    19681 ttcaggaact atatgaagtg atccaatgat tagataatgt aaatgaaaaa ggaatagaaa
    19741 agaggaaact ggatgtatat tcggcaaaca aggaaagaag aagtagaaga agtggtagaa
    19801 atcattaatg ccgcagcgga tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg
    19861 cagaaaccaa caaaagaaac gattttgaac gatattgaaa gaaaagaaag ctatgtatta
    19921 atagtggaag atcaaattgc tggaacagct gcattagtct caagcgtgga tcccgtttac
    19981 acaaaaatag atggtagttg gaaagaatca actggtcctt atttatcgat tcatcgtgtc
    20041 gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg
    20101 acgattgcag ccagcaaaaa tatccaagat atccgtatcg atacttatcc agctaatgaa
    20161 ccgatggaaa agaccatcta ttctgctgga ttcacttatc aagggatgat tgaatttcct
    20221 tttgaacacg gcgaaagaaa agcatatcaa ataatcatca attgactata aaatcatctg
    20281 agcaaagata aagacattaa cactactgaa aaaaacaatc atatgatagc caagaggttg
    20341 taacagaagt ggccagcttc aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt
    20401 ttgtggaatt ttagcgtatt ttacgaaata gctgctttct gaaactccct tcatcgagag
    20461 gtcgtgacat agcttttgtc acgacctctc ggcttatcct acgagtatta ttcgatacat
    20521 ttatatcaat aatccttttt ttattttatc tgcagcttct tggaacgctc catcgtttga
    20581 cacgactaaa agtgagggga tagctttcaa acgtttcaac tgtgctaaat cttctacata
    20641 aaccgccagt ctggctgaaa tctgttcaga tgaatctcca cgttttttca ggcgcgattg
    20701 caatgtttct ttagagatcg taataaagat aggacggact accgtcccga aatactcata
    20761 taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt
    20821 tttaatttct gctttactgg aaccatattt atgttgagca tattggtccc actctgcaaa
    20881 ctcatttccc ttgatcatct gattgaatgt attgtcagaa acaaaataat aatcctcttg
    20941 atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta atgatctttt gttccggctg
    21001 aaaaatagct tctgcgagtg aagtttttcc agaaccagaa ggaccgataa atacatagca
    21061 ataattcatt gtcatttctc ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt
    21121 tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta taccagcaag ccagtcacaa
    21181 tagcacttaa aaagctgact aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga
    21241 caagatttcc tttttcttca tttaatccct tcatcgcacc agaaatgatc ccgatagaag
    21301 agaagttggc aaatgaaacg aggaagacag atataattgc tgttgtacgc tcagtaaaat
    21361 ggaaattacc atttgctaat tctgtcatag caacaaattc atttgtaacc agtttagtcg
    21421 ccatgatact accggcatcc actgcttctt tccatgggat tccgctgatg aaggcaagag
    21481 gggcaaagat gtaacctagg atctcttgga aagtgatgcc gaatatcccg tggaagatag
    21541 cattgatcat ggcaatcaaa gcaacaaaac cgataagcat agcagcaacc gtgatcgcca
    21601 catggaaacc atcaagaatg tattctccca acatttggaa aaatgtctgt tctttgtttt
    21661 cttcaatgat cagctcatct tctttttctt ctaatacata gggatttaca attgaagcca
    21721 gaatataacc gccaaataag ttcaaaacaa gggcagtgat cacatattta gaatcgatca
    21781 gtgccatata agatccgacg atcgacatag aaactgtaga catagccgat acactcatgg
    21841 tgaacaaacg tttctcagat aggaagggta attccttttt tatggaaata aaaacttccg
    21901 attgtcctaa aatagcagaa gctactccat tataagattc caatttgccc atcccgttga
    21961 ttttacttaa tcctagacca acagctttca taaataatgg aagaatcttg atatagcgta
    22021 gaataccgat aatagcagaa ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg
    22081 cacttccttt atttgctaca ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa
    22141 gaagatgatc aaacattttt gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta
    22201 ataatacgaa accaagaaca agctgtaaaa cgaacatcac aattagtggt ttcaatttga
    22261 ttgctttttt gttactgctg atcaaaaaag caagccctaa tgtgacaaat aaaccaataa
    22321 tacctaataa ataactcaaa taatcactcc tattccaaat tccgttttat tttacctgtt
    22381 ttgcaaataa aatactaatc tttagtgaaa ttttcattac ttttttagag tgaaacgcat
    22441 tcattttcca ttagaaaata aagcgaacaa a
//
LOCUS       DASJSZ010000049        22415 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.049, whole genome shotgun sequence.
ACCESSION   DASJSZ010000049 DASJSZ010000000
VERSION     DASJSZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.049"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(141..659)
                     /locus_tag="U4B03_001687"
     CDS             complement(141..659)
                     /locus_tag="U4B03_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN2068540.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(661..1278)
                     /locus_tag="U4B03_001688"
     CDS             complement(661..1278)
                     /locus_tag="U4B03_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN2068541.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1275..2129)
                     /locus_tag="U4B03_001689"
     CDS             complement(1275..2129)
                     /locus_tag="U4B03_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2068542.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2126..3037)
                     /locus_tag="U4B03_001690"
     CDS             complement(2126..3037)
                     /locus_tag="U4B03_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2068543.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3078..4412)
                     /locus_tag="U4B03_001691"
     CDS             complement(3078..4412)
                     /locus_tag="U4B03_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2068544.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4620..5450
                     /locus_tag="U4B03_001692"
     CDS             4620..5450
                     /locus_tag="U4B03_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEN2068545.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5694..6012)
                     /locus_tag="U4B03_001693"
                     /pseudo
     CDS             complement(5694..6012)
                     /locus_tag="U4B03_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6134..6328)
                     /locus_tag="U4B03_001694"
     CDS             complement(6134..6328)
                     /locus_tag="U4B03_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068546.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6833..7018)
                     /locus_tag="U4B03_001695"
     CDS             complement(6833..7018)
                     /locus_tag="U4B03_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068547.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7868..8209)
                     /locus_tag="U4B03_001696"
     CDS             complement(7868..8209)
                     /locus_tag="U4B03_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2068548.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8209..8439)
                     /locus_tag="U4B03_001697"
     CDS             complement(8209..8439)
                     /locus_tag="U4B03_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2068549.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8726..9064
                     /locus_tag="U4B03_001698"
     CDS             8726..9064
                     /locus_tag="U4B03_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068550.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9269..9508)
                     /locus_tag="U4B03_001699"
     CDS             complement(9269..9508)
                     /locus_tag="U4B03_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN2068551.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(9484..9831)
                     /locus_tag="U4B03_001700"
     CDS             complement(9484..9831)
                     /locus_tag="U4B03_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN2068552.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(10563..11144)
                     /locus_tag="U4B03_001701"
     CDS             complement(10563..11144)
                     /locus_tag="U4B03_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2068553.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11327..11953)
                     /locus_tag="U4B03_001702"
     CDS             complement(11327..11953)
                     /locus_tag="U4B03_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2068554.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11975..13306)
                     /locus_tag="U4B03_001703"
     CDS             complement(11975..13306)
                     /locus_tag="U4B03_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEN2068555.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13461..13862)
                     /locus_tag="U4B03_001704"
     CDS             complement(13461..13862)
                     /locus_tag="U4B03_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN2068556.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13948..14154)
                     /locus_tag="U4B03_001705"
     CDS             complement(13948..14154)
                     /locus_tag="U4B03_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068557.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14249..15751)
                     /locus_tag="U4B03_001706"
     CDS             complement(14249..15751)
                     /locus_tag="U4B03_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEN2068558.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15769..16224)
                     /gene="argR"
                     /locus_tag="U4B03_001707"
     CDS             complement(15769..16224)
                     /gene="argR"
                     /locus_tag="U4B03_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN2068559.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16322..16939)
                     /locus_tag="U4B03_001708"
     CDS             complement(16322..16939)
                     /locus_tag="U4B03_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEN2068560.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17160..18533
                     /locus_tag="U4B03_001709"
     CDS             17160..18533
                     /locus_tag="U4B03_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2068561.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18608..19720
                     /locus_tag="U4B03_001710"
     CDS             18608..19720
                     /locus_tag="U4B03_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN2068562.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19792..21429)
                     /locus_tag="U4B03_001711"
     CDS             complement(19792..21429)
                     /locus_tag="U4B03_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEN2068563.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21597..22205)
                     /locus_tag="U4B03_001712"
     CDS             complement(21597..22205)
                     /locus_tag="U4B03_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2068564.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6557 a         4551 c         3890 g         7417 t
ORIGIN      
        1 attataaaat tttataacgt tctcatttaa caaaacttaa gttattaaga caaatgaata
       61 aacaactacg ctttcctcga aacaaaaaaa taaagcacaa atgcgcattt acgcactcat
      121 gctttattcg tacaattaat tcagataacg gcagcgcata ggatgccaat aacacttcct
      181 aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa gtaaaaaaat cctcgaataa
      241 gcgatcaaac aagcaaataa aaaggctaat attcctgcaa ctggacttaa ttcgaacaac
      301 gcagctactg ataaaaaggc tagcgctgta tgtcctgaag gaaaagaaga agtcttcggt
      361 ttacttgtgt aaaggacaag ccgacgattt ttttcataag gtcttttccg tcttgtcgtt
      421 tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac tgccgataag aatggctaga
      481 ccatattctc tagtatcagg tatcaagact aaaacagcag caaggagcaa ccaaataaaa
      541 ccaccattag agatattact gataaatttc aaaaaattga tttttcgact atccgttttc
      601 cgttgcttgg cgaacttgtt gataatacta tcatcgattt tagcaatcaa acgcaacatg
      661 ttattcctcc tttttaagaa ccttttcttg cgtacgatgg ataaaaagca ggacttgaaa
      721 ataaaacaag aaactgaaac ctaataaagc aaaatagatt ggtcctgcaa accagaatgc
      781 gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg cttttgaaat cattaaaaaa
      841 caagaaaaat gcagtaatcc aagaagcttc tttttgttcc agttgaagca agattttgta
      901 actaaagaga agcgcgctta atagaagcaa aaacaacgta gtatacatca atgtatagcc
      961 aattccccca ccccaatagc ggaaaatctg ataatcgatc aatagaagaa gaaaataaac
     1021 acttgctaaa ctatcttgct ttccataaag agagaagttc tcacgaaact ttttccagaa
     1081 tagtgaaccg agctttttct cttcattttt tttgtaatga taaatcatat acagaactgt
     1141 tcgacttgca gttcctaatg tgaaaacagg caagacgcac aggaaccata gtagctgtaa
     1201 atggataaga cgtggaatcc attcaatgat ttctaagaaa taatcattgg cttttttacc
     1261 aatcgatcga ttcatcatcc ttttgaacct cctgtcaaat ccatcccttc aatgaaataa
     1321 cgttgtgcaa aaaagaacag caaaatcgtc gggatcaaga caagtgttgc tcctgccatc
     1381 agataattcc attgtgtcgt tgcttggttc tgaaaaactt gcaagccaat ctgtaacgtg
     1441 tatttttctt gatcttggat atataacaga ggacctaaga aatcattcca agctgcgttg
     1501 aaggaattga tcgcaatggt aatcaaggca ggtttcgtca atggaagcat caaatgactc
     1561 caaatataca aatgattcgc tccgtcgatt tcagcagctt cgatcaattc attattgatc
     1621 gataagtaaa attgacgcat cagaaatata ttgaacgcat tgccgaagaa cgatggaacg
     1681 atcaggggca aataagtacc tacccagccg atcttggaga ataatacata ttgagggatc
     1741 atcgttacaa accccgggat catcatcgta gaaagaacca aagcaaacat cagctttttc
     1801 ccaggaaaat caagcttcgc aaatccataa gcaataaaag aatttgaaag gacattaccg
     1861 atcactacta gcacagtgat gaataatgta tttcttgcat agcgccaaaa aggaaaggcg
     1921 gcaatcgtat ccagatagtt ttcccatacg attttttctg ggaaaaaggt cggaggataa
     1981 cgaacgattt cttccattgg tttcaatgaa gtaaacacca tccaaagcaa tggaatgatc
     2041 aaaatgattc ctattactgt catgaggaca taacttagta ttttttttgt ttgccgattc
     2101 acactggtct ttttgaaata atccatcatt tgccgtcacc tccagaataa tagacccact
     2161 taggtgcaag cttcaaattg acaaacgtca gaaccaacac gatcagaaac aggatcattg
     2221 acatagccat cgcataaccc atatcaaaag ctacgaacgc cttgttccac atatgcagat
     2281 tataaaaaag caaagaattt gcaggtccgc ctgttccatt tttcgtcatg acataagctt
     2341 cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac atcgtaaaaa ataataggag
     2401 tgattaatgg caaagtaata cttttgaact ttccccaaac gcctgctcca tcgatttctg
     2461 ctgattcata taaagatcta ggaacatttt gtaatgtagc taagtaaagt agcattgctc
     2521 ctccagaact ccatattttc atcaaaatca acgcaggttt cgtccattgt ggatcaaaca
     2581 gccaagctgg tccagggata tggaaccagc ccagaaatgt gttgatcaat cctgcagatg
     2641 gagaaagcag ctgcatccat aagatgtaga ctgctactcc tgataagact gccggtaaat
     2701 agaaagctgt acggaataca ctgattcctt tgattttttg attgagtact gtggctaaaa
     2761 acacactgaa aatcgtcgtc agcggtacat tgaagatgac gaaataaacg gtgttgtata
     2821 acgatttcca aaataaatca tcgtgaacga acattctttt gaaatttgct agaccaatga
     2881 aattcatctt ggatgtcaaa ttataatcgg tgaaactacc aataactgaa aaaagcatag
     2941 gccctagcat aaataccaag aacccgataa taaaaggcga gataaataaa taggcccatc
     3001 ttgcttcttt tttctttcgg atagataaac gcttcaaatt ttcttgcctc ctctgtacct
     3061 agacagtcaa aaatggatta ttgattttgc ttgactaagt tttctactgc tttttgtgca
     3121 tctgccaatc cttcttttgg tgattttgtt cctaaaagaa tctggtcaat ctgttcattc
     3181 actaatgtcg tgtaatccgg agcatctaaa ggaactggtg tgatcaccgt atttttaaag
     3241 ttttcgtccg ccatttgata gatcattttt cctttatccg ttaggttgtc attattcact
     3301 aaattctcat tggctgtctt gctcgccatg atatcaaaac ttttttcgcc gaatttttct
     3361 tgtacttctg gagaagaaag atatttgatg aattcataag aggcctcagg atcttttgct
     3421 ccgtatggta cttccaagac gaatccgcca ccccacgacc aatggcctga accttcttct
     3481 ttttctggga gctgcatcac gccaaaatca aaatcatccg gtgcattttc catcaaacta
     3541 gagtaataat tgatattttg ggcacgcatg gctactaatc cggaaataaa tggatcagat
     3601 acaccagacc caaattcggc ttctaattta ttgatcgtat ttttcccata ataatcttgc
     3661 cagtcttgga tccactccaa ggcttccact ttatttttag tatcaatctt gactttttca
     3721 tctttgtcaa accaactagt cccgttatct gcgtttagcg cccatacatc agcccctaaa
     3781 ttccataatg gataaaaacc gatgcgtgta aattcttttc cttctttttt atctagttta
     3841 cgtgctgttt cttctaattc atcccaagtt gtcggtagat cttgttcact gattccagct
     3901 tctttaaata actttttatt ataaaaaatg acttgtgtat ctgtattgaa tgggatcgca
     3961 taaggatcac cttcatattc tactgtatcc caaagctgcg gataaaaatc cttagaagtg
     4021 tctttttcta catatttgga gagattagtt gcttgctgtg cttctgctct ttgggcaacg
     4081 gaatttatgt cctgtacaat aacatcgggt gcatttccag ctgttattgc agataaagat
     4141 ttagtccaga tgtcacccca tggctgatac ttataattta catggatctc gttttgtgat
     4201 tgattgaaat cctcaatgat ttcttcgata gcttcttttc gtgcaccgga tccccaaaag
     4261 gaccagaaat caatattttt tcttccatca cttgtttttt cagaagaatt tgctgcactt
     4321 gaactggcat cgcttgcatt acttgatccg catcctccta ataaaaacaa tgctgaactt
     4381 gccagcaacc ctgtaaacaa taattttttc atattttacc tccaaatttt aatgtaaccg
     4441 atgccggttt taatataacg atatcatttt gttctgaaaa taacaattta taaggtatga
     4501 ttctaaaaaa attattaatg ataccaatac tttatataat tcgcgagcgc tttctttgaa
     4561 ttttttaatc tttcgtagca gaatgagaat gtattaaaaa tagattagga ggaattttga
     4621 tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc agatcgtccg aatccattaa
     4681 tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg cgagatgagt gtagctatgc
     4741 agtacctgtt ccaaggctgg agctgccgag gagaagaaaa atataaagat atgctgatgg
     4801 acattgcaac agaagaaatc ggccacgtgg aaatgttggc gacaatggtt gctcgtttat
     4861 tagagggtgc gcctgttgaa gatcaagaag cagcgatgca aaaagatcca acaatcggtg
     4921 ccattatctc tggaatgaat ccacagcacg cgatcgtaga tggcttagga cctcgtcctg
     4981 ctgacagcgt gggcaatcca tggagcggtg cctatatcat tgcgagtggc aacttgttag
     5041 cagactttag agctaattta aatgccgaat cacaaggaag acttcaagta gcacgtattt
     5101 acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc tgttctcctt gcaagagata
     5161 gctatcatca aaatctatgg gaatgtgcag tgaaagaatt agaagaaaaa gaaggttcaa
     5221 tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa agacattgct tatgacttct
     5281 ataacttctc agagggagac caaagtagtg aaggttcttg ggctagtggc aaagcactgg
     5341 atggcgaagg cgaatatcaa tggttgaaag aacctaagat cgaaggcgct gcaccaaaat
     5401 tagaaccagt aacaccaaaa atgtatggga cacctccatt caagaaataa atatctaccc
     5461 gtagaaacac ccgttttttg ttccacacgc tttgtgagca aaactgacta aagtcattaa
     5521 tcataaccac ccttcaaaag ggtggttttc tctagagcta taagctcaaa tcaccggcca
     5581 gcgcctaaag acgctggctt tcatatttta tgttcaagcc atattgcctt tgccagtttt
     5641 gcccacctct taaaaggttt atgtactact ccttagccct taaaaggatc gtcttactct
     5701 cgtacactca gcttatctaa cgcactatca tgtttttctt gttcttgaat gtactttttg
     5761 atggtcgctt cattttgtcc tacttttgtc acatagtatc cttccaccca aaagtgtcga
     5821 tacccaaatt tatattttag atttgcacaa tctcttgatg atctcctgaa tattggctcg
     5881 atattgatta taaattattt ttcttctata ctttggtgtg aatacaatgt ggtatttaca
     5941 catctacttt gtacgggcta aactatttcc ctttattgct ccacccgcat agcaagtggt
     6001 tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa gacaataaat ctaaaaaccc
     6061 gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa tgaaatgaaa aagatctctt
     6121 acggcaatct attttatttt tttgattttt tataaatgga aaatgcttca agaagtccaa
     6181 gaaataaagc cgtataaaaa caagaaccta tgaaatcttt atttattaga tattcaacag
     6241 aaatcccgat aacactagcc aacaatatat ttatcagaag tgtaaaataa ggatgctttt
     6301 tatagaatag aactagtgtt gttttcatag actctccttt cttcatattt ccaattaatc
     6361 acaaattcac ggcattacta gaaaaattaa aggggcgcag aagtggaaaa atactcttcg
     6421 actttcccta caaaccaggc catcttgtgt ttaataacta taccaaatct aaggtacctc
     6481 taaatctaaa ttccatttgt ggcggtgacc tccaattttt aactcttcta tctcccatcc
     6541 agtatacata accttgatac ttcgaccact tatatatcga acacacaaat acctatccat
     6601 tgcaggagta ctacctaact tagttgctac ttgcatctaa ataacagctg aaaagatagg
     6661 acttactctt agtaaagtat ctgaactatt aatttttaaa ctatcatttt tatcctcaac
     6721 tacatgcaca tctgttgcaa cttcactttc tgtctcatcc agataaatga ttaaggaatc
     6781 gggaattgca atatcttcag ctgacgctgg aatagttgag ctaaattagt catcatatct
     6841 ttattataaa ataaccaaca gcaaaaaata caatgaaaaa caaacttgga actaatgtat
     6901 cattaataaa tgttgaaaaa ttcagcaact cattactgta tgattttagc agcccaacca
     6961 acatataccc tataaaagct aataagatgg ttaggaatcc tttagaattt ttactcattc
     7021 atagacactt ccttaaataa cttttattat agaaggtatt gtaaataata atattgacaa
     7081 aaagtgactc aaaataaaaa aaaccttaca ttatactgga atgaactgta ttgatagtgt
     7141 acctaaaagc tctactgaca taagatcgtg taatcggtaa agtaatttta ttaattatac
     7201 gggatgtaat caattagaga ataaactaat cataaaaggt acttttacat tcataattgt
     7261 cgctttgaga ttctactttg gttcactatc tgacccaatt gcacattttc gatcattaca
     7321 agtattggtg tcgttagaac aactaaataa aaactagcgg tagatttttc agaatctatg
     7381 ctaatttctg tatggatgtt ccagtgatgg caaatttctc ttttcattgt ttttcttgtc
     7441 gcatgatatg cctttttgtg ttattcagtt aaactataga aattctttcc tagacagatc
     7501 ttgatcccaa attttacgtt tttgagcatc gatatagttt aaaatgacat tactcatatc
     7561 taaagagttt agatttttta ggactaaaac tgtcagttgt tctttttctt ctttctgtta
     7621 aagtattgtc gcgacttttc gtatacgtta agatatgtcg tgaaaaaaca agttttgtga
     7681 cacaaaaaaa cgaatgactt gaggaaatca cctcaatcat tcgtttaaaa tgctatatat
     7741 tctttagttt aagatacgga aattaatgcc atcgaccaca ctagtagaat gaacgacatt
     7801 tacttggcct ttttttaatt tcgttatgtt tagcatataa aaatattata gagtttactt
     7861 acaatgctta aaacatatac tgtataatct gatcaatttt agccatgtct tgcaatggta
     7921 agttttctat ttttttgagt tttcgttcct taaaatcaag agattttaat tgtgtaataa
     7981 ttacttgtcc ttttatatct agctcttcag gtaatgagta acgtgtcggt aaattcttta
     8041 tagtggaagt aattggacat atgaccgaaa acattgtttt acggttaaat tcataacgag
     8101 agacgactag tcctggtcgt cttttttgaa tttcttttcc tacggaggga tcaaaatcaa
     8161 tccatacaat atcacctttt ttaggaatat aaattctttc ttctgacact ataaaatttc
     8221 tctcccttca ggagttaaat cttcccattc gtctttctca taatactcgg cctcttcccc
     8281 accactaaat ggatcttcaa tttttggaac taacgtaatt gttccatcat cagaatacac
     8341 aacgatatat tcttggtttt ctgaaggttt ctttccgtta tcagaaggta aagttaccac
     8401 tacagaactt ccttgtaatc tagattttgt aacaagcatt atcatcactc ccttaataaa
     8461 agtatactat ataacactaa gtaacgcaaa attccccttt gtaaactatc tagaaaattg
     8521 ttttgtttaa agtatatctt attggatata aatagaaata aattgtagcc agttcatttt
     8581 ttctttaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
     8641 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
     8701 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
     8761 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
     8821 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
     8881 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
     8941 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
     9001 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
     9061 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
     9121 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
     9181 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
     9241 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
     9301 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
     9361 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
     9421 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
     9481 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcattgg
     9541 gaaaacacaa cgattttgca aaaatggttg atgaaaggtc tttttttctt ctactgtctc
     9601 tgcacgggca ttaatcatca attgtccttt tttaaatccg gtaaatcccc acttcatgat
     9661 ttccggagtg aggatgtttt cttcctcttt tcttaacgtc acaacaagat ttgacggaaa
     9721 aacttctcca gctgctaatt ctgctgcctg cttcggttgt ttcgtctggg cttctcgata
     9781 ataattctga aggagctctg actctagatc aaaaaaataa cgaccacaca tcctaatccc
     9841 tcccaatctt tttttctatc atacactgca acaagaagtt cgcaccaaca aaacgctttc
     9901 aatttttgta gaactaaaaa agttgtgaca agtgacaaac tctccagtga atggtattct
     9961 cctgccaact cctcggtcac aagtcacaat attcaacaaa catgagaagc tgctttttga
    10021 aattgttcct tgtgactcgg agtgaaacgg caggcttacg acctctgggt aaacggtgtt
    10081 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gctattttca
    10141 caaaattttt cttaatactc gaaactaagc gactttttca caacctcttc cttaaacggt
    10201 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agcagctatt
    10261 tttatttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct ctccactaaa
    10321 cggtgttcaa tcagctaacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta
    10381 ttttgccgtt tgtttctcta atactcaggt caaagcgctg atttcacaac ctctaataca
    10441 cggtgttcca gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag
    10501 caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctaat
    10561 tattaaagaa ccttttgcga agttaacaat ctttctttga tcaattgaaa attctgccta
    10621 agtagtgata gatcctgtgt gactttcaac aataagatac ttccctggat ttcagcaatc
    10681 aacgctttgg cttgacggtc tgcttcttca tcggacagtt tttcttcaga aagtgccagg
    10741 atattttgtt tcgtatactt gacccattga ttgactaatt ctagtagcaa cagacggtat
    10801 tcttcatcca ctgtactcaa ctcaataatc aaattaccta caggacagcc atgataagtg
    10861 atctgtgtct catgatcagc gaaaatccag tccaacattt cttctaatgc ttcttctggc
    10921 tgttttccag catttaagat gtcttcgaac aattctgtct gccatacgtc catatgcgca
    10981 tggatcaccg ctagacaaac ttcttttttt gaagaaaagt aataataaaa ttgacctttt
    11041 cctgtatcag aagctgctag aatatctcgg attgacgttt catgatagcc ttttttataa
    11101 ataagatctt tggtcgcttc caagatcttt tcctttgttt tcaatgatat tctccttttt
    11161 catttctcat agtcctatat tatacagtaa attcatcatt ttttcttctt aatcgtttaa
    11221 aaaagactgt gccaaattat ctgacacagc cctcactata aaactgttct catatagcta
    11281 gatgttcatt gattcaagaa ctgcgaggac aaaagcaatt cctatcttat gatagttgct
    11341 ctaatacttc ctctgttgtc atcacttgat ctgcaataaa gccaaagttc gtcaacgctg
    11401 catccattgc ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca acattgaatc
    11461 cactctctac caattcacgc atatgggaat cgctacacaa gtttccgttc attccaccaa
    11521 gaattactgt gtcgattcca ttttttctca attggagcgc caaatcattg ctttctggtc
    11581 caaagatttt atgaggagag ctgatgatcg tattttcatc caaataaggt ttcaattctt
    11641 cgataaaatc ggctcctgaa ccttcttcca ctgattcata ttgacttttt ctagcaaaca
    11701 tctgctgctc aagcatcata tgttctcctg caccgccaaa ttgccattta tggtcatgtg
    11761 ggtaaaaata atgaggagaa ataaagagtt tgtatccttt ttcttttgct tgttttacca
    11821 tttcaatcat attttcgatc gtattgtatt tttctaagat atcttttgtc aaaccaaagc
    11881 caccgcctgc tggtttcaag aactcgactt gcgggtcagt gagaactact gctgtatttt
    11941 tattattgaa catggtggat tctccattcg ttatttattt taatacatca tttagagctt
    12001 cactcattgt cggatgagaa tagatttgat ctctcaataa cgtataagga agttttccat
    12061 tcatagctag cgaaatcaag ttgatgactt catgagaatc ttccgcatag atacttgccc
    12121 ctagaatcaa gtcagtttca gggtcgacta aaactttgaa tacaccttta ggatcttcta
    12181 atacttgtgc tttagggaca ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt
    12241 gtgctttggc ttgtttttct gttaaaccta cattggataa agtcggagtg ataaatacac
    12301 tgtaaggcac agctttccgg ttattcgttg ttcgtttatt ctcgcccttc aactgatcca
    12361 taatgattcg ataatcatct agagagatat aagtgaactg caatccgcct ttgacgtctc
    12421 caatcgccca aatgttgtct gcatctgtac gaaggtattc atccacagcg attgctccgc
    12481 gatctgttag tttaacatct gtattttcta atccaagtga tgctgtactt ggttttcttc
    12541 cagttgcagc caaaactttt gacgcttgga ttgttacttc ttggccatct tctgtaaagg
    12601 tgacggcagc tgttgattct tggtctgcta cttgatctac agatacacct agatggaatt
    12661 cgattcccgc atttgtcatg tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg
    12721 gaagaaattc agaatgggca tcaagaacga caactttaga tccgtattca ttgaacattg
    12781 atgcaaattc caagccaata tatccagccc cgatgatcac taatttttcc ggtaattgtt
    12841 cctgatccat cgcttgcgta gaatcaatca agtagcgact tgtattcaat cctttgattg
    12901 gtaaaatgat tggttcagaa ccagtattga tgaaaatccg ttcgccttca acaatcactt
    12961 tttcaccatt attcgtcact tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca
    13021 tcactgtccc agttgcctca tcgctgatca tatgataatt tttggcgcga agcatgcccg
    13081 tcaatttttc tttccgagca acagcctctg taaagtcgat tcctctttct ccattgaaaa
    13141 tcaatgattt agaaggaata cagccgatgt tgatacatgt tccgccatac atacgtgttg
    13201 acttttcaac gactaaaacc gattcacctt ttgaagctaa cgccttcgct aaagtcttac
    13261 cgccctttcc aaaaccgatc acaatatttt gatatttttc catcattttt ccctcgcttc
    13321 ttttttacct accgattggt acaaaaatat gataaccgat aattgtctat tctccaaatc
    13381 atctgcttta tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt ttttagtctg
    13441 gttcgatttc ctttattctt ctaaacaatc gttacttcta catcgatatt tcctctagtt
    13501 gctttggagt aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg
    13561 ctgatgccat cgatatgcac ttctaatact acaccgatct gatagccgcc atcatctaaa
    13621 ttataaagag agacacgtgc ttttactgtg gactctgctg tcattcctgc ttctttgatc
    13681 atatgttgta ccgccccatt gaaacaagaa gcataacctg ctgcaaataa ttgttctgga
    13741 tttgttcctt ctgcatggat tcctggagga gtgattttca tatgcatcga gccatttggt
    13801 gcctcgactt ctccattgcg cccgccatgg ttgatcattg ctgtttcgta tgcttttttc
    13861 attgtactgc tcctttcgat tgaccatttg ctacttggct ataattatag tgtatgacaa
    13921 gtaaggcgct ttctacaaaa attctgctta ttcgaatacc agaatgccgt cttctactat
    13981 ttttccaaaa aacaaaaaaa tactactcgt gaacaacagc gtcatatttg ttccaaaata
    14041 aatagcaatc ttttctacat aatcttttat atgattgatt ttaagataca tatataccag
    14101 aaagttgacc ccataaacca atactgttaa aaaaataacc atattcgtta ccattttttc
    14161 ctccttttgc tggtctccct accagcactc gatttaacca taccactttt tttgccgtcc
    14221 aagcaactag cctttcaaaa agactggttt attctgctgt taatgattca agatcagaaa
    14281 aatgtccgaa taattttgct aattttacta acgccaactt gttctttccg aattcatcat
    14341 tttctatctg taatactagt aacggctcat gtaagcttaa acggatcaaa aaccagccgt
    14401 ttccgtatgg ctcttctaga tcatatcgaa taccttcttc attttccggg tcgaagttca
    14461 tccctttttc ttcttccaac agtcgcttca tttcttctaa tatcttgttt ccttgttcgc
    14521 gtggttcatc gctgaacaat tttaatcgga tttcttgtac ttctacgggc tgtttcaact
    14581 ctttgatttg ttcatctaat gtgatacctt ttctttttaa ttctggcaaa agcatcagga
    14641 tctttgcgat gacatatgca ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc
    14701 cgcttgtttc gatggctagc tgtgtatctt tcccaagacg gttaagttct aatgctttat
    14761 tgattacatt tcgatagccg gaaatataac gaacttggat gcctcctttt tcttcaatga
    14821 atctttgtag atgagaagaa gtaggggaat tcgtcacgat tcttgttcct ggatgctctt
    14881 ttaatacgat tgtacttaat actgcgatca aattatttcg attgatcact gttccagaag
    14941 cactgacaac tgcagaacga tccacatctg tatcgaaaat gatcccaaga tctgcttcat
    15001 ttgctaaaac agcctgcttg atactttcca tcgcttcttt attatcagga tttgggatat
    15061 gattaggaaa tcggccatct ggttccaaaa attgggaacc tgttgaatcc gcacctaagc
    15121 tattcaatac tttttcagca aagaatccac cagcaccatt tccggcatca acaatgattt
    15181 tccaaccatt tagtggtttg ctttctagtg ttccaatccc ctttttaatt tttgtgacta
    15241 aatcttgcgc ataaggtgtc aaaaggtctg ctttttctac gcttccagtt tgttcgcttt
    15301 ttattttttg ctgctgtgat aggataaaag caatatcttc tttttctgct cctccttctg
    15361 aggtaaagaa ttttattcca ttataataat agggaagatg gctagcagtc agcataaccg
    15421 cagcatcgca cgagaactgt tcaaattgag tggacataaa catagcaggc gtagtagcta
    15481 attcgaaatc gatgacatgt atcccttgtt ctttcagact ttggatcaac gcctctttca
    15541 gtgctggtcc gcttatccta ctgtcatgac caatcccaat cgtccatttt ttatttggat
    15601 cggttgattt cttctttttc tttatccagt ttgctagtcc ccagccgatc ttttttacca
    15661 tgtctgtcgt caagtttact tcatatcctt caactggtaa tgcgattcct cgaatatccg
    15721 atccattttg cagcgctttc atatctttca tataaaagtc ctcctcattt attccctcat
    15781 tttttccagc ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa taataatgaa
    15841 tgtatctaca ccggccatcg ttccgacaac ttcagggtaa gccacttcat ctagccaatt
    15901 cgttaccaca tcggcaccat ttcgttcagt caatacgata accatgaact gaacagactg
    15961 gatacgcaaa acctcgcgtc taattgctgt acgcaaacgt tcttcattta aagaaacaga
    16021 tggctggtta aataatgtat agcgtactat tttgttttca ttttgacttt ttactaactt
    16081 catttctcga atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag
    16141 ccgcgcaagc agctcttcct gtgtcccaat aggttgttct gtaatgatct ggcggatcag
    16201 tgcttgtcgc tgtttctttt tcatattaga cacctctcaa tgttgattat agcaaaagat
    16261 aagtaatatt tcttcatatt ttggtatttt ttacttttat tttttgtttt cattcaggtt
    16321 gctaaccttt ttctatttta acacgcaaaa tcgtcttcat tttttcaggt gctgattttc
    16381 ttggatcgct gatgtaaatc tctttgtgtt ctttagaagc tctttcatag ccttcttttg
    16441 cgatgaatgc ttctaatttc tcaaacgttg ctggttcttc ggcatagggc ccgacgtgta
    16501 agatttgggc acaaagacct tcttccatct ttttgaatac tacttgttct ttcaagtctt
    16561 ccggaagttt gctagaacgc tgaatggctt cttgtgctat cgcttctgta acaaagtcag
    16621 gctgtcggat catcaattga taagaaaaat gttctttttt catcactggt tcatgcaaat
    16681 acttttcttg cagtccccat tgtccttcca gtggatagac agtatacatc tgataatcag
    16741 gaatcagcca atttttttta ggcgacatgc gtattgcata gctgactgca tatagacagg
    16801 ctactcttcg tttaaagtct tcctgattcg ggtcgccgat ccctctgatg ctaaagaatg
    16861 attttttagg aacctctagt atttgcgggc tttttttcat actatagctc ttgtctttac
    16921 gccattcaat tttttccatt tttcttcctc cgcgaaattc catttagtta tttattatca
    16981 tacctctttt tgagtgactt ggctaatcgt gttgacacta tttttcagtt tgtgctagaa
    17041 taagtgaagt aaataaatcg tacagataac acgtttcggc gtgtcgagag agaggcggtt
    17101 tctattttta taatagaaat cactctcttt ttttacgaaa ttttttagag gtgatgtaaa
    17161 tgacaaaaca aaaacagctg cttttagtac ctgcagtaat tgcaacaggg atgatgtctt
    17221 ttgcgggcgt attgatcgag acggccatga atgtaacttt cccaactctt atcagacagt
    17281 ttgatttaac gactgcacaa gtccaatggg taacaacgat ttatcttttg atgatttcga
    17341 tcattgttcc gctgtctact tatttgaatc gcaattttag tctcaaaaaa ctctttttag
    17401 cttcaaatct gcttttcttg gcaggtataa tgatcgattt cttctctcct accttcatgg
    17461 tcctattgtt tggtcgattg ctccaaggtg cagcgacagg tatcgctctt ccgttgatgt
    17521 tccatattat cttgacattt gcccccctca atcgacgagg cgcgatgatg ggtgtcggta
    17581 ctttgacaac ggccattgct ccagcaattg gccccactta tggaggcatt atgacttcac
    17641 attttacatg gaatcatatc ttcctgtttc ttgtgcccgt tttgtttctt tctttgatta
    17701 tcggctggtt tgctattccc gagatggcag tacaaaaaag tggttatttg gattggataa
    17761 gcgttttagg aatttgtctt ttgtttagtg gaagtctaac atttttgagt acgttgggaa
    17821 cagtttccag ctggattgct ttaatcatcg gcggtattgg ctggttcctt ttttactatc
    17881 gaagtaaaaa agtggagcat ccactgatcc gactgtctat tctttcaaat aaaacattcc
    17941 gcttgttttt gattggcttc ttagtttatc agtttttatt acttggtgta tcttttgtct
    18001 tgccgaattt ccttcagatc gttcaaggaa gtagtccttt tgtcgctgga ttagcgatgc
    18061 ttcccggcgc agcggttggt gctttgcttt ctcctttttc cgggaaaatg ctggatcaat
    18121 atggtccgaa gaagccaatt tttgctgggc tgattctttc tttgattggt tggctagcat
    18181 tattcctact cataggacac gcttcagtgg gtgtattgat tgcttgtcat gtgttttata
    18241 tgatcggtat cggattatct tacagcaata tgatgaccac cggaatgaat gcactgaatg
    18301 aagaactaca aggagacggg aacgcaatgt tcaatacctt gcagcagttt tctggcgctg
    18361 tggctacttc attagtagcg gtcattatca attacgtcca gcatcatact tcccataact
    18421 atgaagtgtc tactacatta ggatcaaaag cagcattagg cgtcttgttg ctgttgcttc
    18481 tcgtaagctt tgctcgattc gcctattatc tgttctttgc taaaaaagcg tagattccat
    18541 atacggcagg tgttgcatct gccgtttttt tgtgtgaaaa tggttgtttg taaaaggagg
    18601 cttttttatg gaaaaaattg ccattatcgg tggcgggatc attggtatga ccctagccag
    18661 ttacttagat acatcaaaat tcaatgttgc tttatttgat gaagggacag gacaggcaac
    18721 taaagctagc gctgggatta tctccccttg gttgtcgaaa cgcagaaaca aacaatggta
    18781 tcgtctggct aaagacggcg ctgctttttt ccctaaactt ataaaagatt tccaacttga
    18841 tcaaacgatt tacgcacaat cagggacact tttgcttcga cctgaaaaag atttagctga
    18901 attggctgtt cttgccgaga aacgaaaaga agaagcgcca gaaattggaa acatcgaatg
    18961 gctttcacct gaacagacat ctcaagcact tccgttattg aaaccagcag tttcactgcg
    19021 tatcagtggt ggtggtcgtc tggatggaaa agcatattta gaacatcttg gtcagcgttt
    19081 agcacaaaaa caaatggagc agatccaggc aaaagtcact ttgcataaga agagcgattc
    19141 ttggctcatc aagggaccgt ttggagaata cacagctgat cgtgtaattt tgacacctgg
    19201 cccggcactt aagcctttat tacaagaatt aggctatgct gcagatatac gtccgcaaaa
    19261 agggcagctc ttagcatttc agacatctta taagatgagt caagactggc ctgttgctat
    19321 gcttgacgga gaagctgatt tcattccttt catggatgga aagatccttt taggtgctac
    19381 ccatgaaaac gatcaaggct gggatttggc tcctactagc gaagcttata agggattgac
    19441 taagagtaca gaacagttct tgactgatcc agatcagata aaaaactggc catacgagtt
    19501 ccgtgtaggt acccgtgctt atacgtcaga tttttcccca tttttcggcg tactgccaga
    19561 tgatccaagc ttactggctg ctagcggtct aggctcttct ggcttgacca caggtcctta
    19621 tatcggctat ctattagcca acttcttaaa tactggtcat tggcctacgg aagattatca
    19681 aaaggacatc tccacctata ttacgaaaaa tccatcatag aaaaacagct ttttcaagta
    19741 aggatcccga gcgatattgc gcatggatta cttcttgaaa aagctgttat tttagttcaa
    19801 tttctgaatc aaattctcca aactttcttt tgcttcatct ggcaagagat cttttcctat
    19861 ctgagcttca atgaatcgta aacgaatatc aagtcgttgt ttcaccagat ttttatagga
    19921 cgtatcctca aaagaaacag gaaaatctgg tataggtaaa tagtggatgt cttcaaatgg
    19981 accaaacgac ttgaattctg ccgtttgttg cacgattctt tcaacgattg acaatgtcgt
    20041 ttcaggtttg atttttttat catcgaaata tccagtattc aggacatagc aggctgtttg
    20101 ctgtttcaaa aacaactctc gaaaagcttg atagtcttcc ctaagaggat agacacggaa
    20161 aggattagca aatggttcaa tgactagctc atctagctgt ctttttgtag tgatattttc
    20221 cgcgtctgat cgttttgttg ctaatgttgc accaaaagac gctgctaaaa ctgggtcatt
    20281 gatcttgata attggcggaa gcgaatcatc tttcatgatc caaaaaacag catcgatctt
    20341 ttcgctaaga tgatcgactc ggtcgggtgt cgcataacgt gatttgactg tccgcccatt
    20401 tccgtttcgt atatctcctg ttactaggac tttttctcct ttacttgtac atgtcacgcc
    20461 cacattctga caagtaagga aagaagaaat ggccggatca tcagacggat agtcttgtgt
    20521 tttatcaaag taagaaggtt ccaaggcagt agcaaaacca ttttttcgag aaataacgaa
    20581 agcatcgtca tgcaagatcg aaacagagtg accttccgta tgttttgcta aggtaatcgt
    20641 tgattttcca gaaccggaca acccaaatac agccatcgta taggtctggt cattactagc
    20701 atattgtttc atcccaccgt ggcaggcaat aaagccattg cgatttccca atgcccaagc
    20761 aagcgtcaaa gtcgcttttt ttaattctcc aaagtaacgt aatcctagca cagccgctgt
    20821 attatggaga gcatcaaaca aaatgagtcc atttggaaat gtttcatgtg tccattcggg
    20881 atcacaaaag atataaagat ctccctcatc aaaggaaaca gacttttgat aagtctctaa
    20941 gatcttcgaa ttcgctggct gaaaattcag caaataagaa tacaaattgt tctcaaatcc
    21001 ttctggaatc agcaaatgac tttgcacaga aaattcttca gataggccaa cgattacttt
    21061 tgtatggtaa aactggcgct tgcgactttg atacacagct tctcttaaaa ttttctcgta
    21121 tttgctttgt tccttgtcta agtcctcaaa gatccgccga gcacttgctg ttcttcccac
    21181 aatgcttcca tcattgaata ctagtgtcct aacatcagct ggtaaatcta gttcttcagc
    21241 atgtgagatt gttaaatctg tgatgatcgt tcctggactc ccttttgcta gttgataagc
    21301 tgtcttccta tcaaaaactt catgaacatt attaccgtaa aatgtcgttt ctacaatcga
    21361 tcgaatactg gataacagtg gatttccact tgtgatttcg tttaattcga gactctcttt
    21421 tgtactcatg gctcctcctc attccctaaa caaaaaaacg ttttctttat tatacattat
    21481 tcgagttttt atttgtactg ctgcgctgtg ggaagcaaac gagttatttt tcacacaaaa
    21541 aaaccgagaa cttgaataaa caagtttctc ggtttcatca ttttcctttt atcggcttag
    21601 taccagccgt ttgctaacca gaatgtttta gcagcatccc atgaaccata acgagatgtt
    21661 acatattgtt ctgctacacg ttcttgattg gcagctgaat agtcgccgtt taagtatgaa
    21721 gcatctaatt gataacggcc gatgtaacgt ccgttagtag ctgtgtatga accgcctgat
    21781 tctttttgtg cgatccattc ttttgcagag cttgaagttg gcgctgttgc agcagctggg
    21841 gcagcaggag ctacttcaac tgtttcaaca ggtgcaacgt atgtatgttc ttgcgttgct
    21901 gtttcagttg tagatgctgt attttctgtt gttttgtttt ctgttgttgc tgaagaatca
    21961 tttgaagttg ggattgttaa ttgttcacca acataaatgc ggttgatgtt tgcgatcgaa
    22021 tttttttcag caattgcatc gatcattgag ttgtctcctg caaatttttg tgagatctta
    22081 gataatgaat cacctgattt tactgtataa acttcatcag cgtgagcatt tgtagttcct
    22141 acaaacattg cagtagcacc agcagcaaaa gttgtaccta aaacaatttt cttaaatgaa
    22201 ttcatattaa actccttttt ttctccgttt tttattcttt cgacgctatt aactttatca
    22261 tttagaaata tttcataggt aaaagaaaca tgacatttaa tgacaaaata acgaattatt
    22321 taatatttgt aacatttaaa caaatatttt tttgatacaa aaccataaaa gccttgttgt
    22381 ataagcaaag taatcgtttt tctatgtttt tttat
//
LOCUS       DASJSZ010000050        22165 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.050, whole genome shotgun sequence.
ACCESSION   DASJSZ010000050 DASJSZ010000000
VERSION     DASJSZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.050"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="U4B03_001713"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="U4B03_001713"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN2068565.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="U4B03_001714"
     CDS             2370..3848
                     /locus_tag="U4B03_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2068566.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="U4B03_001715"
     CDS             3814..5145
                     /locus_tag="U4B03_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2068567.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="U4B03_001716"
     CDS             5259..7811
                     /locus_tag="U4B03_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEN2068568.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="U4B03_001717"
     CDS             complement(7818..9257)
                     /locus_tag="U4B03_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2068569.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9603..9965
                     /locus_tag="U4B03_001718"
     CDS             9603..9965
                     /locus_tag="U4B03_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEN2068570.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10152..11009)
                     /locus_tag="U4B03_001719"
     CDS             complement(10152..11009)
                     /locus_tag="U4B03_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2068571.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11129..12040
                     /locus_tag="U4B03_001720"
     CDS             11129..12040
                     /locus_tag="U4B03_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN2068572.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12047..13698
                     /gene="mdcA"
                     /locus_tag="U4B03_001721"
                     /pseudo
     CDS             12047..13698
                     /gene="mdcA"
                     /locus_tag="U4B03_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13800..14099
                     /locus_tag="U4B03_001722"
     CDS             13800..14099
                     /locus_tag="U4B03_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEN2068573.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14089..15765
                     /gene="mdcD"
                     /locus_tag="U4B03_001723"
     CDS             14089..15765
                     /gene="mdcD"
                     /locus_tag="U4B03_001723"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEN2068574.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15769..16362
                     /locus_tag="U4B03_001724"
     CDS             15769..16362
                     /locus_tag="U4B03_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEN2068575.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16386..17462
                     /locus_tag="U4B03_001725"
     CDS             16386..17462
                     /locus_tag="U4B03_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN2068576.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17487..18356
                     /gene="mdcB"
                     /locus_tag="U4B03_001726"
     CDS             17487..18356
                     /gene="mdcB"
                     /locus_tag="U4B03_001726"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEN2068577.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18467..19231
                     /locus_tag="U4B03_001727"
     CDS             18467..19231
                     /locus_tag="U4B03_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEN2068578.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19720..21813
                     /locus_tag="U4B03_001728"
     CDS             19720..21813
                     /locus_tag="U4B03_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2068579.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21838..22140
                     /locus_tag="U4B03_001729"
     CDS             21838..22140
                     /locus_tag="U4B03_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2068580.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7179 a         3787 c         4722 g         6477 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10201 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10261 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10321 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10381 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10441 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10501 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10561 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10621 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10681 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10741 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10801 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    10861 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    10921 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    10981 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11041 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11101 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11161 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11221 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11281 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11341 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11401 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11461 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11521 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11581 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11641 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11701 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11761 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    11821 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    11881 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    11941 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12001 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12061 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12121 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12181 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12241 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12301 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12361 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12421 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12481 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12541 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12601 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12661 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12721 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12781 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    12841 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    12901 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    12961 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13021 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13081 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13141 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13201 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13261 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13321 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaatgca
    13381 ccagtgatga tttatagcga agatactaca catatcgtga cagaagaagg aattgcttat
    13441 ttgtacaaag caaaaacaaa agaagaacga caagcagcaa ttgcagcaat tggcggtgtt
    13501 acacctcttg gaatgactag cacgaaagaa tcactagata gcttgcgtgc agccggcatc
    13561 gtgaaattac cagaagatct aggcatcaaa cgtagtgaag cgaaacgttc attattagct
    13621 gcacaaacca tcgatgattt gatcgaatgg tcagatggcc tgtatgaacc accaatgaaa
    13681 ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg tcgccattta cccagatgga
    13741 agaagaaaga cgaataagca gaacgtgtat gctctgtttt acaaaaaggg gagagatcca
    13801 tggaacaatt agcttataaa tatccaacga aacagccgat caataagaaa atccatgtag
    13861 gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc aacagaaaaa acagaatcta
    13921 ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt atggaacaat gtattaacga
    13981 gattttttga tcgttatcca atttcagcag atatcgtgat caatgacttc ggtgctacac
    14041 caggagttgt gtacctacga ttgacgcaag caatggagga actttcagat gaaaaatagt
    14101 tttgtagaat taaacgccag agaacgagtg aacgctctac ttgacaatgg acgtgaattg
    14161 attgatccat tcgatcaaat catcgcgcca agtttaccag cacaaggaat cgtccctgaa
    14221 agtgatgacg ggatcgttgt cagccgtggc acgatcaatg gcaaacaagc agtagtaatc
    14281 gctatggaag gaaaattcca aggtggcggt atcggtgaag tttccggtgc aaaaatcgtt
    14341 gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa atgagatctt tccagtcatt
    14401 gtactagata caggtggtgt tcgtttgcaa gaagcaaact acggactgtt atctatttca
    14461 gaaatcggaa atgtaatcat tgctctaaaa caatatgtac cagtcatcgg tttgattcca
    14521 ggacgtgtag gatcatttgg cggtatgtcg attacttcag cattaatgag ttatttaatc
    14581 gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag ttattgaaca agaagcaggc
    14641 gttcgagaat ttgattctag tgacaaagac ttgatttgga atacattagg atctcgtcaa
    14701 cgggtagaat caaaaatcat tgatgaatta gtgactgata gtgtagaagc aatcaaagct
    14761 gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc gcagtgaaag ctcagcattt
    14821 tatttatcat tacttgattc tttagatctt tcaaaaccaa tggccattga cgattacaat
    14881 gaagcaattg ctaaccatca agcaaaatca attgacttac cagaagtagc agaaggaaat
    14941 gaaccagctg cagcaagtgt tggttacgat tggtttgcgg cactaactgg catgaaaaat
    15001 ccaacttctt ctattagtac tgtgtattat ggtaaatcaa gtaaattcat agaagaagcc
    15061 atcgtgacat caatcgtacc tgatcctaaa aacccgatgt accgtgtacg aaacggtgaa
    15121 gttggtttag ttgaaggttt ccgcatggga caaattttgg atcacgttta cgaagaagat
    15181 aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg atgtaccaag ccaagcatac
    15241 ggctataacg aagaattgat cggtatccat gtggctttgg ctaatagtgc tgctgcttat
    15301 gcaaaactcc gtcaagctgg acatccagtt attggattga ttgcaggaaa tgctatttca
    15361 ggtgcatttt tagcacatgg attacaatca agccgcttga ttgctttaaa caataaagaa
    15421 attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg tgacaaaacg ttcggttgct
    15481 gaaatcgaag cagctgctga aaaagttcca gctatcgctt acgatatcaa taattataca
    15541 aaactaggag cgatctatca tttcctagaa gatatcaccg accaagctgc ttcagaacaa
    15601 aatgtcagca aagtcatcga agcaatcaat gaagccattc aagatgtccg caatacaaat
    15661 gacacgatgc tgacaaatcg atacacaaac gaaacagcca aaacattcgg acgtgtcgaa
    15721 acaaataaag tccgtgccaa aatggacgaa gaatgggatg cataaaaaat gattcaagca
    15781 catgatattg tcctttttga taaacatgaa atagaagggt tcaccttgcc tgaatgggtc
    15841 gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt cgacggatca cgcagaaata
    15901 ctaccgattg gtttacgtgg acctgcccgc aatcagagat ggtcaacaga aatctcaaaa
    15961 catgccgtca aacaagtcat tcatccgtgg gacatcatcg aacaagaatc gtatagacaa
    16021 gagaaaatca tggattatcc tgtttatcag caatttcatg ctgctagaaa catattatca
    16081 ggatgtaaat ggggaatcgg cggcagctta ggatttgaat tatcgactgg gattccggca
    16141 gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa attcaccgat tgaattgcca
    16201 attcaggtaa ttcaatcgca tcctgctttt tttgagcaat tcgataccca agtagtgact
    16261 cggcaaggcg gtttttcact aaaagagtat ttgagagagc cagagaaaaa aattctattg
    16321 aaaacagtcg aaggacctaa attaaccaaa gaactttggt aataaagaaa aagagagaga
    16381 aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag tgacttccag aaagtatcag
    16441 ctgtaacggc tgttatgatg caaacgatat tgagctttgc tttggcaaac agccacagcg
    16501 caacaaatac agcttttctt ggtacctttt acgtcatcgt taaatacaca gcaccaatgt
    16561 ttatttttgc aattgtttat aatatggtaa aaacaagtca gcatttgtct tattgggaat
    16621 ttttgaaaga aaagtttttt gaactagtcg ttccgtatct tctatggacg acggcttatc
    16681 tttggctatt tccagcagtc caacaacaaa ctccgtatac ggatactgca agctttttac
    16741 tgaaatatgt cacaggtgat ggagcgcctc atttatggta taccgttatg atgctgcaga
    16801 tccaattgtt catgccgttt tttgtttggc tcggttacaa agtatttgct aataaaaaat
    16861 atgtatggcc agtattgatt ggttcaacga ttttatatgt cgcatggtat ctgttctatc
    16921 aaacacaagt attgactggg ccttatcatg acagctggta cttattagat cggtttgttg
    16981 ctagtttcat gatttatggg gtagccgcat ttgtttatca cgaaaaagtc tatcaatatt
    17041 tggacagagt acgttatctt ttcttacctg ttgccttagt tatcgcgttc ttttctgtcc
    17101 gttcattgct cgcacatcca ggagacttga gctttgcgaa tgcaccttac ttgaatacga
    17161 tccaaagttt atacagttta gtcatcatct ttgctgtctt cataggcgct tccaaaatga
    17221 ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt gtctgtttat gcttatcgta
    17281 cgtatttagc caatgtattt gttttccaag tactgttatt attgtttaaa gatagttggc
    17341 tgcagttacc ttcaggagtc atgattcttg tagcttatct aatgacggca agttgtgcat
    17401 ttgctttaag ctggttactt catatcattt gggtagctat caaaaaaggt ttcagcaagt
    17461 aatagtagga agaaggagtg agtgtcatga taaatgacga acgaaaaaaa gcagtgactc
    17521 agcaactcgc tgcgttggta gaggaagcat tgatcgcaga agtaacatta agtccaaaac
    17581 ctggattagt ggatgcactg gatgcgggtg cacattccga tatggactat gctttatttc
    17641 ttaagtctgc taagacgcta actccttttt ttgaagaaat ggctcaaata gcatggcatc
    17701 atgctattga tcaggagtta agagaaagaa ttgctgagat tgggcgaaaa gcggaaaagg
    17761 caatgctgat tgctacaaat ggcatcaata cccataaagg agcgatttgg gcaatggggc
    17821 tattgatcag tgtcattgca caaaaaatca gtatggaaca gcctttttct ttatcgaaaa
    17881 tacttgatga tgtcagcaag cttgctgctt ttcctgatac aaaatatcgt cataaaaaaa
    17941 ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa tggtgcatat gaagaagcga
    18001 tattagggta tccgcatatc aggcttgcaa ttcaagcagc taaggagcta gataatgatt
    18061 catatgtaca gaaacaaatg catatgttgt tagttttgat gagttcgcta gatgacacat
    18121 gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat gcaaaaactt gctcatgctg
    18181 ctagtcaaag tgctttacca aatcgtccat ttacagcact gttgcacttt tgtcaggaaa
    18241 accaaatctc tcctggagga agcgcagatc tcttgtctgc aagcttgttc atgctgaaga
    18301 tagaggaatt atggactgcc gacacataca tttgtccgat gttactcaat gactaaataa
    18361 gacaaaattg ctaaaaaaac agtttatgca tctggttatt gactcttcct tttagcaagg
    18421 ctatcataaa aatagttttt tgtaataaaa gataacgagg aagacaatga aaaaaatcaa
    18481 ttatttagca atcattttgt tagtgggcct atgtttgttt gctctgccga cccaaggatt
    18541 cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt cgaagttcgg gcagaggttc
    18601 atttggtggt cgttcatatg ggggcagtta ttttggcagt cgttcttacg gcagagattc
    18661 ttatgggtat tatggtccag gtacaggtcc ttctagttat gagcaaacgg gatttcttgc
    18721 attagctgtt ttgtcagtag gaggggtttc tgctttttct atcgtcaaga ataaaagaca
    18781 aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca ggaacggcta aagaaaagaa
    18841 agcacagttt ctggaaatcc aaaagatatt catgaagatc caatccgctt gggaagaaga
    18901 aactcctgaa aaagccagaa gctgttatac agacaagctg tttgccgaac atcagaaagt
    18961 tctgcaaaaa aacaaagcag acggtgtaag aaatcatact aaaaaaataa ctattcagga
    19021 agtaggaaat tatcgacaga tccacgaaaa tagttttgcc atccgaattg atttcagatg
    19081 ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc tcaggaagta agacacaacg
    19141 acagtatttt tctcagatat ggtattttga tttttctgag aaagaaaaaa gatggcaagc
    19201 cgattttatt cagccgatta tgctggattg acaggaaata tttatgaacg gagttgtgac
    19261 aaaagccttg tcgcagctcc tttttctgaa taaacggtgt gccagagata gcctatcggc
    19321 aataaggcac gattttgtaa aatatgggaa gcgatttttc aaaatcgctt cttatcgctc
    19381 aaggctgaac atctctgtca caacctctcc actaaatggt gtttaaaagc ttgcatctgc
    19441 gataataaga acaaccaaac gaaaaatagg aagcactatt ttagtttagt tgttcttatt
    19501 acttgatgca ggacagcttt ttcacaacct cttttttgta tagatgggca aaatttttgt
    19561 aataataggc gtatgtcttt ttcgttgagg agatgaaaaa gcatatggct tattttttgt
    19621 ttgctttgct tactttcttt ccacttcaag tggcagaaat atctactttt tattatgtaa
    19681 gcggaaccat atacttgaaa tcagaaggag atcatttcta tgaaaacaag aacaattgaa
    19741 atacttcaca gaatcatgac agcccaatat ccagtaaaag caaaacatct agcaagtgag
    19801 ttcaatgtca gtcagcggac gatccgtcag gaagttcttg aagcgaatat ctggttgagc
    19861 agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag gttttttgtt gaagctggaa
    19921 aaggaaaagc aagctcgatt agaaactgaa ataaaagatg tgaaagacgg ctttttgaat
    19981 cggcgcgaac gtatttttga tctagtttta gccgttgctt acagccgaat cccgatccat
    20041 ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta cattagatga agatatgaga
    20101 cggttacgag caacactggt cagttatggg atagagatcg tcagttatgg aaagcaagga
    20161 ctggtttata aaggttcaga acgatctatc cgtacgatga tctatgattt tatcaatcag
    20221 aatctgggaa tgaccgactt ctttatgaat caagatgcac aattaacacc ggctcaaaaa
    20281 atctttctcc attattttcc tttggtggaa atcaaaaaaa tacaggagat ctattccaat
    20341 aagttgttga aacaagaaga tgatatttat aaaaaacaaa tcctgctttt tacattgatc
    20401 tggatccagc gaatcaggaa acatgaattt atctctgcca ttaattggaa aaatacagaa
    20461 ataaaagaca gtagcttttc tgcatttatt gaccacatga tcgaggaatt tcaattggac
    20521 aatgtcccgc aagtagaaag aaattatctt cgtttcacta ttgagacgtt caatgctaga
    20581 gatatctcca attcaatcga gtgggtacaa gctcaattac taacgatcca attgatccaa
    20641 tttgtagaga acgagacaca cgtacctttc catctaaagg aagaagcttt attcgaaaat
    20701 ctctataagc acatggctgc actgattgta agaatcaaga atcatatcca agtcatgaat
    20761 ccattgaaag aaaacatccg aacaaattat ttcccaatct accgcgcagt cactcatttt
    20821 gttcctacga tcgaagaagt aattggtggc aagatactgg aggacgagat agcttttctt
    20881 gtcatccact tttcgacgat cgccagtgta atcaagcgag acttgaatta tgtttataaa
    20941 tcagtcgttg tctgtaacca tgggatggct actggaaatc tgttagcaga gaatctgaaa
    21001 gaaaagtttc ctcagatcga gattactgca gttttaagtt caaaagaaat cgaattagta
    21061 gataaattgg atgtcaatct ggttttcagc acctttcaat tgaattatcg aaataagccg
    21121 cttttagttg tcgatccgat tttaactgag acgaatatct tggttataga agactttttg
    21181 aaaagaaatg aagaactaca gcggttagag cctaacagtc atgaatcgac ggagttattc
    21241 actcaaataa tagatgtcat gaaagaaagt ggtggccgag tagatcgaat catctatggc
    21301 aagttagaaa aattattcga aacaaacaat ttagaaatca acaaaaggga gatccagcct
    21361 atgttaaaag atattttgac ggacagcaat atcatgatca aagaaaaagc cactacttgg
    21421 gaggaatcga ttgaactagc tgctagacca ttggttaaag aaaacattgt cgaagagcgc
    21481 tatgtcgaag cgatgattcg tgcagtgaat gattacggac cgtatatcgt gattggcaaa
    21541 cacatggcac tagcccatgc ccgaccagaa gatggggtca ataaattagg agtcagcgta
    21601 gctacgattg agcaaccaat cgatttcggt aatcctgaaa tggatccagt aaagattatt
    21661 ttttgtttgg cagcagtaga ctcctactcg catctgaaca tcatgaaaga actaattgaa
    21721 ttgatcaaca acgaagagaa gttggatcga ttgattgaat gcacaaatat cgaagatttc
    21781 aaacaactgc ttttttcagt agttgagaaa taataaatga tacgggggag aacaaagatg
    21841 aacaaattga acattttatt cgtatgcggt gcaggtttag gaagtagttt tgcagcacag
    21901 atgtctgctg aggatgtttt gaacaaatta ggagtagaag ccaaactgga ccattgtgat
    21961 atttcttccg cagtttccat gaaaccagat gtgatcatca cagcagaaaa ttttcgttcg
    22021 cagttcgaaa agttcgcagt tgatcctaaa acggcgatga tctatttgaa aaatattgtt
    22081 tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc aagataaggg agttatctaa
    22141 atacaaaaag aaaagagggt aaaaa
//
LOCUS       DASJSZ010000051        19629 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.051, whole genome shotgun sequence.
ACCESSION   DASJSZ010000051 DASJSZ010000000
VERSION     DASJSZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.051"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="U4B03_001730"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="U4B03_001730"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN2068581.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="U4B03_001731"
     CDS             complement(1172..1867)
                     /locus_tag="U4B03_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEN2068582.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="U4B03_001732"
     CDS             complement(1880..2596)
                     /locus_tag="U4B03_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEN2068583.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="U4B03_001733"
     CDS             2926..3534
                     /locus_tag="U4B03_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEN2068584.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="U4B03_001734"
     CDS             3859..4182
                     /locus_tag="U4B03_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN2068585.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="U4B03_001735"
     CDS             4364..5734
                     /locus_tag="U4B03_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEN2068586.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="U4B03_001736"
     CDS             complement(5864..7165)
                     /locus_tag="U4B03_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN2068587.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="U4B03_001737"
     CDS             complement(7519..9477)
                     /locus_tag="U4B03_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN2068588.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="U4B03_001738"
     CDS             complement(9654..9920)
                     /locus_tag="U4B03_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2068589.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="U4B03_001739"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="U4B03_001739"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEN2068590.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="U4B03_001740"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="U4B03_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEN2068591.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="U4B03_001741"
     CDS             13264..13827
                     /locus_tag="U4B03_001741"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN2068592.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="U4B03_001742"
     CDS             complement(14080..14601)
                     /locus_tag="U4B03_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEN2068593.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="U4B03_001743"
     CDS             complement(14604..15446)
                     /locus_tag="U4B03_001743"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN2068594.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="U4B03_001744"
     CDS             complement(15439..16164)
                     /locus_tag="U4B03_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEN2068595.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="U4B03_001745"
     CDS             16340..16636
                     /locus_tag="U4B03_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068596.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="U4B03_001746"
     CDS             17204..18796
                     /locus_tag="U4B03_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN2068597.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="U4B03_001747"
     CDS             18809..19549
                     /locus_tag="U4B03_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2068598.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5617 a         4028 c         3724 g         6260 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttc
//
LOCUS       DASJSZ010000052        19395 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.052, whole genome shotgun sequence.
ACCESSION   DASJSZ010000052 DASJSZ010000000
VERSION     DASJSZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.052"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(39..635)
                     /locus_tag="U4B03_001748"
     CDS             complement(39..635)
                     /locus_tag="U4B03_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2068599.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            985..1329
                     /locus_tag="U4B03_001749"
     CDS             985..1329
                     /locus_tag="U4B03_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN2068600.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1330..1749
                     /locus_tag="U4B03_001750"
     CDS             1330..1749
                     /locus_tag="U4B03_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEN2068601.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1841..2191)
                     /locus_tag="U4B03_001751"
     CDS             complement(1841..2191)
                     /locus_tag="U4B03_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068602.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2157..3488)
                     /locus_tag="U4B03_001752"
     CDS             complement(2157..3488)
                     /locus_tag="U4B03_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEN2068603.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3743..4309
                     /locus_tag="U4B03_001753"
     CDS             3743..4309
                     /locus_tag="U4B03_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2068604.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4454..4541
                     /locus_tag="U4B03_001754"
     tRNA            4454..4541
                     /locus_tag="U4B03_001754"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4486..4488,aa:Ser,seq:cga)
     gene            4877..5851
                     /gene="bsh"
                     /locus_tag="U4B03_001755"
     CDS             4877..5851
                     /gene="bsh"
                     /locus_tag="U4B03_001755"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN2068605.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6176..8071
                     /gene="asnB"
                     /locus_tag="U4B03_001756"
     CDS             6176..8071
                     /gene="asnB"
                     /locus_tag="U4B03_001756"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEN2068606.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8266..8712)
                     /locus_tag="U4B03_001757"
     CDS             complement(8266..8712)
                     /locus_tag="U4B03_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN2068607.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8940..9089
                     /gene="rpmG"
                     /locus_tag="U4B03_001758"
     CDS             8940..9089
                     /gene="rpmG"
                     /locus_tag="U4B03_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2068608.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9177..9695
                     /locus_tag="U4B03_001759"
     CDS             9177..9695
                     /locus_tag="U4B03_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2068609.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9772..10728)
                     /locus_tag="U4B03_001760"
     CDS             complement(9772..10728)
                     /locus_tag="U4B03_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2068610.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10894..11700
                     /locus_tag="U4B03_001761"
     CDS             10894..11700
                     /locus_tag="U4B03_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068611.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11697..12686
                     /locus_tag="U4B03_001762"
     CDS             11697..12686
                     /locus_tag="U4B03_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN2068612.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12683..13687
                     /locus_tag="U4B03_001763"
     CDS             12683..13687
                     /locus_tag="U4B03_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN2068613.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13819..14361
                     /locus_tag="U4B03_001764"
     CDS             13819..14361
                     /locus_tag="U4B03_001764"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEN2068614.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14381..15079
                     /locus_tag="U4B03_001765"
     CDS             14381..15079
                     /locus_tag="U4B03_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN2068615.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15100..15312
                     /locus_tag="U4B03_001766"
     CDS             15100..15312
                     /locus_tag="U4B03_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEN2068616.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15312..16274
                     /locus_tag="U4B03_001767"
     CDS             15312..16274
                     /locus_tag="U4B03_001767"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEN2068617.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16292..16687
                     /locus_tag="U4B03_001768"
     CDS             16292..16687
                     /locus_tag="U4B03_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2068618.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16850..17215
                     /locus_tag="U4B03_001769"
     CDS             16850..17215
                     /locus_tag="U4B03_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEN2068619.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17212..19023
                     /locus_tag="U4B03_001770"
     CDS             17212..19023
                     /locus_tag="U4B03_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEN2068620.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19085..19252)
                     /locus_tag="U4B03_001771"
     CDS             complement(19085..19252)
                     /locus_tag="U4B03_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEN2068621.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         6278 a         3298 c         3944 g         5875 t
ORIGIN      
        1 aacattgtca atcaatgact aattttacaa aataaatatt atccaagaag accacttaga
       61 tattgacctc cataaatcaa ggccatacta tataagaaaa tcgacagtgg tttagcaatc
      121 aaaatgatta gtgtatatcg tttcactgac atgtttgtca atccagccat caaacataac
      181 gcatcgtcag gagcaactgg aagaaaaata gctagagcaa acagcttctc aaaacggttt
      241 tgattatcca gccagccaat atatttgtta tatgtcttat cgctaatcat ggaaagtatg
      301 aatggctttc cataacgacg ccctagcaga aaaatgataa tcgaaccaat gcagattcca
      361 atatagttat agataaatcc agcataaggg ccaaatatca ggacaccagc tgcagtactg
      421 atccctccag gaatgattgg aataactact tgcaggattt ggatgaagac aaaaataatc
      481 ggtcctaaaa tgatggaatc acctactaat cctcttaacg cattcaaatc tttgaaaaca
      541 cctaaattaa taaaataaat cgtcagcgcg attgataggc ctaatccgat aatggatatg
      601 aaatttatca gttttcgaga ggcagcaata ttcattattc atcaactcct tagaggttct
      661 ttgttaacta tactttacta gaaaagcaac taactgcgcc attttttgat atgacagaaa
      721 acaaacaatt ttgtaagttc atcataagta taaaagattg atctcaaaaa aacatccaac
      781 taaagactta tttaataact tgtttttttt aaaataaaaa aagcgttagc aactatgctt
      841 aaaaagcata tcgactgaat aaactaagca ttggacaggc tgaaagacta catttaaaat
      901 gaaatgcgaa gaaacaatag aaaataaagt tgccttagaa ccatagttga atcacctaac
      961 agtcgtgaag ggagaataag agaaatggca gaaaaacaaa ccgcaggacg agatcaatta
     1021 ggagattttg caccaaaatt tgcagaatta aatgatgatg ttttatttgg acaagtatgg
     1081 tccagagaag aattatctgc tcatcagcgt agtatcatca ccatatctgc attaatttca
     1141 ggaggaaatt ttgaacaatt gccagctcat ctgaagattg gcaagcaaaa tggcattact
     1201 agtgaagaaa tagcagaaat cattacccat ttagcttttt atgtagaatg gccaaaagct
     1261 tggtcagctt ttagtttagc aaaagaaatc tatttagaag aaaatagcga agataagaaa
     1321 gaggcgtaaa tgatgacaaa acacgaagaa atacacaatg gcgtgatttt tccagcaggt
     1381 gaaaaaaacg aagcttacgc tcaagtattt accgggcaaa gctatctgaa tatgttagtg
     1441 aatgatcccg aagtttctgt tggtgtaggt aatgtaactt ttgaaccagg ttgtcgcaat
     1501 cactggcatg tccatacaga cggctatcaa atcttacttg tgaccggagg agaaggctgg
     1561 tatcaggaag aaggaaagcc agcaagacga ttaatggctg gagatgtcgt cgtaacgcac
     1621 aaagggatta aacattggca tggtgcgaca aaagacagtt ggtttgaaca tttggcgatc
     1681 actgctggta attcagagtt tttagaacct gtaacagatg aagtgtatga aggattggaa
     1741 aatcaataat gtcaagcttg aacagctcct gaaagtagat tttaatcgaa ctttcaggag
     1801 ctgctttttt gattcagtct tttataaata gaaaataggg ttaataatag gaatgagaag
     1861 cacgtcgaaa gatcttaata ttgataatcg gctgggcata catgatttcc acgtcatcaa
     1921 tcaaactacc taacagatga taatcttccc gatcatgtcg gggattacct aatagttctt
     1981 gatagtagat ttccatacta gggtcccgag gattgttcag caaatcagca agtacatcta
     2041 aatcttctgg ctgagtttct gccgtaccct gtatctctat gaaaagccct tcctcagcaa
     2101 attctactcc aatacgtatt tcagttattt catgggaata taaataagta agaagctcat
     2161 cgatgattct ttttgataat tctttttcca agtgtctttt tttcctttct gacagcggta
     2221 aagttatcta gtatcggtac aaggatacct gcaacgaaac cagttgaaaa accactgtta
     2281 tatagattta gtcctccgtg caaagtgctg acgttatgga caagagcaat atgtagcaca
     2341 ccagctatta caccgtaaaa actgccgtaa aaaccgctta tgggcgcaag acctgtccca
     2401 aacacagctg caacaagcat gccagtactg gtgatctcat gaaaagttcc aaacaaagaa
     2461 gcaataaata ttccgactag tatcggaaaa ctgtttttta aatgacaacc aaaagcactg
     2521 aaacccaccg cagataaaat agcaccgatg acaggtccat ttaactgacc gccaaccagc
     2581 aaaacataag aagacagcat cagaccaact aagcccatgt tgatcaaagt agcaccaata
     2641 ccagaattag caatgaaatc agttgtcaat tttccagatg aatcgaaaat ttctcgaatc
     2701 cccgaaagcc gaaaggaatt ataatagaaa ccaattccga ctgtcagcga aaaaaagcca
     2761 aaaataatcc aaatgagagg aaagtgatat tcattgaaaa ccaatgtatt ttcgacgatt
     2821 gaatagctga ataacctcaa aacagcagta aaagtcatcg caatcaaccc agaggtgaat
     2881 ccaatattat aaagtgtgaa tccacgatgg aaaactaaag tttgagcagc taagggcgga
     2941 agaagtatac caattgctat gccagctaaa ttccccaata aaatacctat cagtaaagga
     3001 aaacgcatac caaaagtgat ataactaacg accggtgata atacgctacc aaacagtccg
     3061 atcatgatgt actgagaaaa aggtttattg acaaatacag cgtatagata cacacctaaa
     3121 atgattgaga tagaattata aaggtttttg ccaaaaaaag agaaaccggt aactgttaga
     3181 atcgaagcaa ataagggacc gtttaaaatc accttgtgcc gataagctaa aagcacagaa
     3241 agtaaggtca aaatccctac atttatgaat gcgcttccga aaacacctaa agcaaaataa
     3301 tctgtcaaaa gattgcttgg cgaggtaaga atagttagca atccactcca attatcagta
     3361 ggagtattag aaagcaagcc aataaaaaga aaaaaaacac ctgtaaaacc aagataaaaa
     3421 tactttcttt tctgtgaaac gattcgtttt tgaggatgaa tataaataac gtcagtttcg
     3481 ttttccatta cacaccttct ttttcatttt ctttaactat aacttgaaat gaaagatttg
     3541 gacaactatc atttaggtca aaactaaaaa tgtaagggaa cccattgaat ttaaccaaat
     3601 ttttttaatt ttgcaaaaaa aagttaaaga agactgaaaa aaatttaaaa tataacgttt
     3661 tgagtaaaat gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac atatactgca
     3721 gtaaagaagt gggggagttt taatgagaag aaaagtctat accaaggatc aaattctaaa
     3781 agctgcttat gatgttgttg caaaggaagg gtttaaagga tttactgcga gaaatattgc
     3841 taaaaagatg gggatttcga cacagccgat ttatctggaa ttcaaaaaca tggaagattt
     3901 aaaaaatacg ttgctggatt ctatctttga acaactaaaa gaagatgttt tccctattga
     3961 acgtactggt gataaaatca ttgatctcgg tttgaactat atctactttg caaaaaagca
     4021 tcatagtctt tacatggcat tgtacattga tgaacatggg gacggaaaga aaatgcatga
     4081 tttttcatac aattatttcc gtgaaatagt taaagaagat gaaagataca atcaattatc
     4141 ggatgaatat gtcgatgctc tgcatactgg tacatgggta gcagtgactg gcttagcgtc
     4201 attgatgtca tcaaacatca tgcatccaac agaagaacaa atcattgcac tgatcaaacg
     4261 gacaattgac tcgattttag cattagataa tcctgaaata atcagatagt gttctatcag
     4321 tatgaatttg atcggtaagt ccgttggctg caaactgtca atggactttt ttgctaaaaa
     4381 aagcgatttc tgttatttat ctattagaaa taggcttgac atttcgtgaa aaaagtttaa
     4441 aaatagtact tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac
     4501 cggctaatac cggcgcgcag gttcaaatcc tgtaccttcc ttatatcaac gattagaggt
     4561 tgtgaagaag ctgtcctgca tcaagtaata agaacggcca aacaaaaata gcttttccta
     4621 ttttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg
     4681 aataaggggc tgtgacaaga cttttgtcac agccccttat tgttgtactt ttaacctaca
     4741 atattttttg attccccctg agtactattt tttataaatg ctagtaaatc aatcttcttt
     4801 ggcctttttt ttgataaaaa aaaatactag atgtaagata aaagtgtaaa aacgatttgt
     4861 tttaggaggg aaaacgatgt gtaccgcaat cacttatgca acgaaagacc attattttgg
     4921 aaggaatttt gattatgaga tgtcttataa tgaagtagtc actattactc caagaaatta
     4981 tcgtttcgat tttagaaagg taaaaaattt agataaacat tatgcaatga taggaatagc
     5041 agctggggta tccaactatc cactctatta tgaggctaca aatgaaaaag ggttgagtat
     5101 ggcaggactg aattttcctg gcaatgctga ttataaagaa ttacaagaag gaaaagataa
     5161 cgttgcacca tttgagttta ttccatggat actagggcaa tgctcaacca tagacgaagc
     5221 taaagaatta ttggctacta tcaatctagt caatatcgat tttagtgaaa aactaccctt
     5281 gtcaccttta cactggttat tagctgataa agaaaaatcg atagtaattg aaagtatgaa
     5341 agatggactt catttatatg ataatcctgt cggtgtgttg acaaataatc cgccatttga
     5401 ttatcaatta tttaatctga acaattatcg ctcgctatca aatggaacac cagaaaatca
     5461 tttttcaaac cagatcagct tgaatgttta tagtcgtggg atgggaggat tgggcttacc
     5521 tggagatctc tcttctgttt ctcgatttgt aaaagccaca tttactaaaa tgaatgctgc
     5581 atcaggtgat tcagaatctg aaagtatcag tcaatttttt catatactag gctcagtcga
     5641 gcaacaaaaa ggagtatgcg atactggaga aggaaaatac gaatatacga tttattcttc
     5701 ttgctgtaac gttgataaag ggatttatta ttatcgtacg tatgaagaca gtcaaattac
     5761 tgctgtaaat ataaataaag aagacttgga tcgtcatgaa ttggtcagtt atccagtcat
     5821 aaaagagcaa cagataaact atgtcaatta attaatgaat agatccgttt ggtatcatat
     5881 tttctgatac agtcataatt agacacgatc tttaggtcgt gtcttttttt gctgtttttt
     5941 ttggaaatag catccactaa tattttttta acacgaagag aataaggtag cctcaaaaaa
     6001 gaaaacatgg taaattagat attgggactt tattttctgt aaaaacagaa actttcatct
     6061 tttgttttca cgtgacgaaa ttgtaagaga gaaaatattc gtccgctatt tattctatga
     6121 taagatgcat atgaatgaaa taaacagtaa ttttatatta ggatgtgaaa gttaaatgtg
     6181 cggtattgta ggttttgtta acgacaagga caacaaaaaa acaattatta atacaatgat
     6241 ggatcgaatc gttcaccgtg gacctaatag ttcaggagaa tatatcgata aacatgttgc
     6301 tttaggattc agaagattaa gtattattga cttagagggc ggtactcagc ctatttacaa
     6361 tgaagatcgg acaaagatca ttatttttaa tggcgaaatc tataattacc agcctttgag
     6421 agaagaattg attgcagctg gccacgtttt tcaaacacat gcagatacag aagtcctttt
     6481 gcatggttac gaagaatggg gaacagaatt attgcaaaag atccgcggaa tgtttgcttt
     6541 tgctatttgg gataatgaaa agaatgagtt attcggtgca agagaccact ttgggatcaa
     6601 accatattat tatgcagaaa tgaacggaac atttatgttt ggttcagaaa tcaaaagttt
     6661 tctgcctcat cctgatttta acaaagaatt gaacaaagaa gcgttgaaac cttatatgac
     6721 tttccaatat tctccattaa acggcgaaac attcttcaaa gatgtctatc gtttgcctga
     6781 aggccattat tatacgtata aagatggaaa actagacatt cagcaatatt gggatgcaga
     6841 ttttgaaaca aaagaaacgc attcccgaca agaatggatc gaaaaaatcg acgaaaccgt
     6901 tcaagcttct attgaagcac atacggtcag tgatgtagaa gttggttctt tcttatccag
     6961 cggggttgat tccagttatg taacctctgt attgaaacca gatcattctt tctcaatcgg
     7021 gtttgatgat aaaacctata atgaagcgat tgaagccaga aaactaaccg aactattgga
     7081 cttagataat acagcagcag tgatcgacgg agacatgtcg ttcaaagcat tccctttgat
     7141 ccagtatcac ttagatgaac ctgattcaaa tccttcttgc gtacctttgt actttttagc
     7201 aaatcttgct tcacaaagtg tgcgggtagt ccaatctgga gaaggggcgg atgaactgtt
     7261 tgcgggatac caaacgtatg gattccatac gaattcaaaa ttcatccgtg tgattgctca
     7321 aggattgaaa aaattaccaa aaggaacacg ctataattta ggacgtaaaa tcggaaaaat
     7381 gaaaaatttc catggacgga tccatttata tgaatcactt gctcctgcaa aagaatattt
     7441 tatcggtcat gctcgagtgt tcgaagaatc agaagcatca gaagtgttaa caccaaaata
     7501 tcaaacagca ccatctgtgg atgaaatcat gactgttcat tacgagaaaa cagaaggaat
     7561 caaagatgaa gtcaataaga tgcaatatgt cgatcttcat caatggatgc caaaagatat
     7621 cttgttaaag gcagataaac tttctatggc aagttctttg gaagtccgag tacctttatt
     7681 ggatatcgaa gtcatgaaat tggctcaaca aataccaagt aagtttttat tgaatcaaaa
     7741 caacacaaaa gatattttca gacaagctgc gaacaaacat cttccggaag aatggtcgaa
     7801 tcgtgtgaaa ttaggattcc ctgtgccaat caaagcttgg ttgaaagaag aacacggcta
     7861 cgaacaagtc aaagcattat tcgaagcaga ttttgcaaaa gaattttttg atcaagaaaa
     7921 aatcatgaag ttgctagatg accatcacga aggacgtaaa gaagagcaaa gaaaaatctg
     7981 gacaatcttc agtttcttga catggtataa agtctacttt gtagatgaaa cgattccgca
     8041 agcagaagca atcgactatg tgactgtttg atgaaaatca aatatcaata aaaaagaggt
     8101 tttctcctac cgtttaacac tgagtgataa gaaacaattt caaaaaacag ctcttcccgt
     8161 tttatagata ttatgactta tggttcggag ttggcagagg aacaccattg tttcgaacat
     8221 aagggggttg tgacacgatt ttcgtcacaa cccctttaca tatgttcaat aatcgatttc
     8281 tttttctttt gtcccatctt ttttataaat gaacaaagtt gattctttgt tttgtgctac
     8341 ttgctttcct ttttcgattg cttctttttt tgtatcaaaa tggttgcttg ccttttttgc
     8401 ccctttagat ttcacgatcc attgttcttc ctcatattct acgatcacat ctgaatcaag
     8461 cagttttcca gcacgaggat tcgtatcgtg ttcatccgtc ttgcttggat ttttttcttt
     8521 ttcgaaagtt cgtttttctg attcactagc atcttcatac catttttttg cttgatcaat
     8581 ggcaattggg atcgcacggt tatcagggta cccttcatct aataatgcat ttgcgatgtc
     8641 gattgctttt tttcttgtca atttatctaa gtttttcatt gatgcaggaa agtccttcat
     8701 gttccatggc atattagtat ccccctttat taaaaaacat aatctatata aacaagtgta
     8761 agggtattta tacaaaaaga aaaagatttt gtttgagaag taaaaccgtt gtatttatcg
     8821 tcaagtagtt ttacttaaca aaatcgtgaa aaacactagc aatctagaaa aaatcatggt
     8881 aagataatca agtctgagag tcatcttaga ggattcttgt aaaaatggga gggaaaaaca
     8941 tgcgcgtaaa catcacttta gagtgtactt cttgtaaaga acgtaactat ctaacaagca
     9001 aaaacaaacg taacaatcct gatcgtttgg aaaaacaaaa atattgccca cgcgaacgca
     9061 aagttacttt gcaccgtgaa acaaaataat gtttttaaag ttctcaattc cttgctggtc
     9121 aaagaattga gacttttttt atctcaaaac atctaaaatc ataaggagcg gataatatgt
     9181 ttatttccat gtgggcacaa gataaaaatg gtctgatcgg caaaaatggt ttgttaccat
     9241 ggcgattgcc aaatgatatg cgtttctttc gcgaacatac aatggatcga attttagtga
     9301 tgggaagaaa gacctatgaa ggaatggggg atctttcact tccttatcgc catatcatcg
     9361 tgttgacaac gcaaccaaat tttcgcacga aagaaaatgc ggaagtaatg cattcgatcg
     9421 atgaactttt gtcgtatagt caaacgatct cagaagatat ctatgtttcg ggtggtagtc
     9481 ggatttttca agaacttctt ccttacactg gtcggatctg gcgaacattg atcgatagca
     9541 cgtttgaagg cgatacgtat attgggaata ttgatttttc agacttcgcc cttatagagg
     9601 agtatgaggg gataacggat caagaaaatc tatatgccca tcgttttcaa aaatgggaaa
     9661 gagtaaaaaa tagggttgct tcaaaagaag aataaaatct atgcgtctag tttgttaata
     9721 agaaaaaaag catctcacga ctaatcgtga agattctttt tttagttaaa atcaatttag
     9781 tttttctttg atatcattga ccatttcctg actggcaata ggtccgttat atagccaact
     9841 agattcaggt ccgaattgca gtacatgatt gtctgtcact gctttaagat tcttccagct
     9901 tggttcattg aacatggcag catctttgtc actgttcaca aggatgatgt agtcagcatc
     9961 taattcagct agcttttcag aggaaacagc agaccagtca gaagtggctt ttttggagac
    10021 ctcttccact agatcaggaa caccaaattc cagatcgtcg tatacgattc ttccacttga
    10081 ccgatttttg ctgaccataa aggctgagtt gttggtcacc cacaacactg cagctgtctt
    10141 gttctcgatt ttatctgcta gttcttctcg agttttcttg atggattttt gataatcgga
    10201 aatgattttt tctgcctctt tttcttttgc caaagctttg ccgacatctt ttagttgttc
    10261 ttcccatgtg acatctgttc cgttttttac aacataagtt ggcgcgattt tgctatattg
    10321 ttcgtatttc ccgccttcga cagtagcgga agaagaaatc aacagcaaat caggctcaaa
    10381 gcttaacact ttctcataag gaaggtcata agaaatcgtt gggacatcct ttaaatcatc
    10441 ttgcagataa tcttgaatgg aaccactgcc aacagtccac tgggcgatag gtttttcccc
    10501 tagtgcaatc agatagtctt ctaagtaact gccgatgatc cgtttgggcg cgtttggaat
    10561 ctctacttta tgtccaagtg tatctgtgac tgtatgagta ctattggatg aagtcgtcga
    10621 attggcagtt gtagctgttt ctttatctga agagggggag caagcgctca gtactccagc
    10681 cagcagcaat atgctagata atgtgaatac ttttgttttt aatttcattt ctttcctcct
    10741 aacataattg agaatctttc tcattggtta ttattcctga tatttcgaac gctgtcaacc
    10801 gcttcatttg gctatagata tgaaagtagc gcttgacgaa atgttgaaac agcgtcataa
    10861 ttgagaatga ttttcagcga aaggatgggt gaaatgacga gattaagagc agctgacatt
    10921 gcagccggct acgaaaacaa aaaaatcatt gaaaacatgt ctgttacgat tcctgaaggg
    10981 aaaatcacta gtttgattgg gccaaatgga agtggaaaat ctactttatt gaaaagtttt
    11041 acccgtatac tacctgtgaa atcaggaaag atcattgtca ataactctga tatctctaca
    11101 tacagttcaa aagaacttgc aaaaaaaatt acattattag cacaaactag tgaacaccct
    11161 acaggtatga cagtagaaga agtagtatct tacggacgat acccttatca aaaactgttc
    11221 agtggcatgg acacgcaaga tcatgaagca atcgagtggg cgttggatgc tacaggtcta
    11281 gctggatttc gtatgcggga tatcgcaacg ctgtctggtg gacaaaccca gcgtgtctgg
    11341 atcgctatgg cgcttgctca ggaaacagat atcttgatct tggatgaacc gacaacattt
    11401 ttagatccgg cacaccaatt agagatccta cagttattga ctgaaatcaa tcaaatgaaa
    11461 caaacaacga tcttgatgtc gattcatgac attaatcatg cttcacgttt ttcagattat
    11521 ttattagcaa tgaaaaacgg taagttgatc acagaaggta cacctgaaga agtgatcaca
    11581 aaagagtgta tgaaaaaaat atttgagatt gatgcaacga ttgtcacgct tccaagaaca
    11641 aataaaccag tttttctaac ctatgatttg ttgccaaaag agcaggagga tcaattatga
    11701 ggactatgaa gaaaaataga ctcctgcttt tggtctgttt tgttcttttg attatttttt
    11761 ttgttgctct ttcaaatggc gcagcgactg tgggaaaagc agatttttgg caggcgttgt
    11821 ttgattttga tagatcaaaa caaagtcacc aaatcatacg aaatattcgt ttgcctcgtg
    11881 tggcgggagc atttcttgta gggagttgtt ttgcattgag cggtgcattg atgcaaggag
    11941 tgacaagaaa tcctttagca gattcaggct tactaggaat caacgcgggt gcttcgttgg
    12001 caatggccct tagttttgct ttttggccta aagcttctga tttttctgtg tttctaatgt
    12061 cattggcagg ttcttttgct gtaactttgc tcgtatttgg ttttttgcag ttttcgaaaa
    12121 tgggcatgaa tcctttacag ctgattttag ctggcgtagc tattagttct ttttttactg
    12181 ctatcagcca agcacttacc caattcttta atctgcagca ggatcttgct ttttggtttg
    12241 taggcggagc tgccaatatc acatggaaac aattagcagt attgtttcct ttatatatac
    12301 ttgctgtttt ggtttctttt tcattaggaa aagcaatatc tttgattcat ttgagtgaaa
    12361 cacatgcatt agctcttgga agtcatccta aacgaacgcg gttgattgtt ttctgtctag
    12421 ttgcagtgtt agcagccatt gccgtttcgt tagtcggacc tgtttcattt attgggctgt
    12481 tggttccgcc agttgttcgc ggaatggttg gaacggacta tcgttatgtt ctacctgtct
    12541 cttttttagt tggcggtgga ttggttatgt tggcggattt tacagcgagg atgatcaatc
    12601 ccccttttga aacacctttt ggattagtca ttgcattgat tggcgttccg tttttactga
    12661 tacaagtaag gagggaacaa cggtgaaaaa aatatattgg acgttctatt tcttgctttt
    12721 tgtacttgtc atcctgtcta taggtagttt aatgattggt acgcctttta tttacctgga
    12781 tcagcttttt gccacgttca tagggaaaga atcatcttct ttggcgctga ttgttagaga
    12841 atttcgagtt cctcgattac tgatcagttt attagcagga gcttgtcttg gcgtttctgg
    12901 ttatatcttg caaggagtaa caagaaatga attggcagat tctagtatat tgggcattaa
    12961 tgccggagca ggatttttag tgatgcttta tttaggtttc ttttcgcact attcgctgcc
    13021 gtttctattg ctagttgtag cctttctagg tggtgtttta gctgcttgct gtgtctatct
    13081 tgcggcctat gatcgaaatg gctttttggg tatgaatcga atcttattgt ccggtattgc
    13141 cgttaatgcc ggcctttctg cattaacatt gctatgcacg atccaattat ctaaggaaaa
    13201 ttatggattt gtgaatgcat ggttagcggg atcaatctgg ggtgcgagct ggccttatgt
    13261 ttgggcattg cttccatggg cggttatctt aattccgcta ggaggacttt atgcacagag
    13321 gattggattg ctatcatttg gtcaagaacg cgcgcaagga ttaggcctta acgtggctaa
    13381 aagtcaaaaa atccttttgt tgttagctgt tgctttggca agcggtagtg tagcggtgac
    13441 aggtagttta tcttttgtag gactactagc tcctcatgct gcgaagctag tcgtacgtaa
    13501 agaaaataac tggacctttg ttctgagcgg tttgttcggt tccatttttc tgcttagtgc
    13561 agatatggtt gctagagtga ttctaccaag tggggaagta cctacaggaa ttttgattgc
    13621 cctttttggt gcgccatatt ttctgtatgc actttttatc agacaaaaac acgcgctgaa
    13681 cagttgaaat ttattaaaaa aggaaggcca atcatttcta ggtcttcttt ttattctatt
    13741 tatgcattta tttgaaagtt cgattgctat ttttcttttt ttcctatacc attttatgag
    13801 agaaggaagg gtgagttttt ggaccaattg aaagtaagat tacgccggaa aggaatcgaa
    13861 aagctgaaaa atctggccaa aagtggcaag aaaaaggaaa aagaacagcg aatctataaa
    13921 cagctattcg ccagcgagta ctggaagtca gcaaaagtga tagggacgac tttatcgaat
    13981 gaatttgaac tggatacaca gccggttatc caacgagcag aaaaagaaca aaagaaaata
    14041 gttgtaccta aaactttgcc gaaacgtcag atggcgtttt atgaaatgga tgaacagtcc
    14101 gttcttgtac gttcgagttt tggtgttctt gagccacaat cagagaagct ctatttgccg
    14161 gagatgatcg atctcctcat tgtaccaggt attatctaca atagagccgg ttatcgaatc
    14221 ggttttggcg gtggttatta cgaccggtat ttaaataatt ttcataaaaa aacatgtagc
    14281 cttgtttttc gtgaacaatt gaatgaacaa tggcaaccgg agagctttga tcagaagatc
    14341 caacaattgt ttattgacta aaatgaaatg ggaggttttc atgaattatc aacagcaaat
    14401 aaaattgcgt gcatggttaa gacgaccgtt atggacctat gcttttttag ggatacagac
    14461 agcggtcttc ctcatgatgg aattatttcc gcgacttgag atcccttatt atgccggaat
    14521 gtatggtcct tatcttgttc acttcaatga atggtggcgt ttagtgacac caatcttcat
    14581 ccattttgga ctgatgcatt tcgtaatgaa ctcactgata ctgtatttta tggggcagca
    14641 aatcgaggca atctatggcc attggcgttt tttcttgatt tatatgttca gcgggataat
    14701 gggaaataca gcaagctttg cttttaacga agcgaatgtt ctgtctggtg gtgcgagtac
    14761 ttcgatattc ggtttgttcg gtgcgttgtt tatcttaggg tttcatttta aacataaccc
    14821 agcgatccag cagctagtca gacattttct tttatttatt gtgttgacat ttgtttttgg
    14881 tcttctggat acctctgtag atgtttgggg acatgtcggt gggctaattg gcggattagt
    14941 gttagggaat gttttgggtc taccgaaaca acagacaagc tattcgatac accaaaggat
    15001 tctttctaca ctagtttttg tatttctttt tgtaatatgt attttactgg gtttaaaaaa
    15061 atatggttta cttgtataat atgaaggaag tgcttaaaga tggagagctt atatgatgta
    15121 cagcagctgt tcaaaaaatt tggcatcttt atctatgtgg gtgcacgcat ctatgacatt
    15181 gaattaatga ctatcgaatt gcgtaatctg tatgatagcc gtttgattga tcatgataca
    15241 tacatgacag cgtggcgcat attaaaaaga gagcaccgaa ttgaagaaag ccgaaagaaa
    15301 ggaaatttgt aatggacaag aagattattg ggattgattt aggtggaaca acagcaaaat
    15361 ttgcaatttt aacaccagaa ggagagattc aacaaaaatg gagcatcgat accaatattt
    15421 tggatgatgg taagcatatc gttcctgaaa tcattgaatc aatcaaccat cgtttgaatt
    15481 tatataatat gaaagccgaa gactttatcg gaatcggcat gggtacccca ggaagcgtgg
    15541 acagcgaagc tggaacggtt attggtgcgt ataacttaaa ctggacagaa gttcaatttg
    15601 tgaaaaaact gatcgaggca ggaacaggaa tcaaattcgc tatcgataat gatgcgaatg
    15661 ttgctgcttt aggtgaacga tggaaaggtg ctggagaaaa cgatccagac gttgtattcg
    15721 ttactctagg aacaggtgtt ggcggtggta tcgttgctgg cggaaatttg attcacggcg
    15781 tgagcggagc aggcggagag atcggccata tcactgtgga tcctgaaggg ttcgaatgta
    15841 cttgcggtaa acgcggatgt ttagaaactg tgtctagcgc tactggtgtg gtacgtctag
    15901 gtcgttactt ggcagaagaa tacgcaggag attcaaaact gaaagcaatg ttagacaacg
    15961 gtgaagaagt aacaagtaaa gatatctttg aaatggctca agaagacgat ccgtttgcat
    16021 tgatggttgt tgatcgcgta tgcttctact taggtttagc ttgcgccaac ttggggaata
    16081 cattgaatcc gtcaagtatt gtgttaggtg gaggagtatc ggcagcagga gaatttttac
    16141 gtagccgtgt agagaaatat tttaaagaat ttactttccc gcaagtaaga gaatcaacaa
    16201 aaatcaaatt agcagagctt ggtaatgaag ctggtgtcat tggtgctgcc tcattagcct
    16261 tgaaatttgc ttaaaaaaac ggaggcatta catggtttta tgggtaatta atatcatttt
    16321 gctattgatt gtgcttgcaa tcatcttttg ggaagtctat ctacgagtaa tggccaagcg
    16381 ttcagcaacg actttgacag aagaagaatt tcgagaagga atgagaaaag cacaagtaat
    16441 cgatgttcgc gaaaaagatg tgtttgatgc tgggcatatt ttaggtgcaa gaaacatccc
    16501 ttacactgtt ttgaaagatt cattaggatc tattcgaaaa gaccagcctg tctatattta
    16561 cgatcaaaag aaaagcttga gcatccgtac agcaaataaa ttacgtaaag caggttatca
    16621 agagatttat ttacttaagg gtggatatga cggatggtct ggaaaaatca aaagtaaaaa
    16681 agtataaaac agatgattgt atgacagctt taaaaaccga atcaatcatt agattttgac
    16741 caagatcaca tgaacggcgc aatggatagt tgtgccgttc atttttttta ttttgtgcta
    16801 ttatctacat atataagcta agcgaaaagg cagaaagaag gaaacaacta tgatcaaatt
    16861 aaaacgtgtt taccttgatg ctgaaaaaga ggatggctat agaatactcg tggataggtt
    16921 ctggccacga ggaatttcaa aagaaaaagc gcagttagat ctgtggttga aagaaattgc
    16981 acctagtaat gaagcacgca aaacttttga ccatgtacca gaacgatttg cttcatttgc
    17041 aaagaaatac cagcaagaat tgattttcga agaagaaaag aagcaagcta tttttaaact
    17101 cagtcaactt atgaaagaac atccagtcat tacttttgtt tatggtgcaa aaaatgaaca
    17161 tcagaaccat gctgttttat tgaaaaaatt tatcgaaatg gaggcatgca aatgaaaata
    17221 gggatcattg gtgccggacc tagaggatta agtatagcag aaaggattct ccataataat
    17281 caggacagac attctcttga actttttctt ttcgatccca atggtcctgg aggtcgagtt
    17341 tggcggttga atcagccacc ggagttatta atgaattctg tttctcaaca agtgacgctt
    17401 tttacagatg aaacattgtc cagtggagga ttggtaacac agggacccaa cttgtttcaa
    17461 tggagtaaag gacctgctac agattatatc gaacagacgg taaccaaaaa taagcaacta
    17521 attatagaag aaatcaatcg tctggaaaaa gatcagcaat ctactcgatg tttatacgga
    17581 ctctatcaac gctggttttt ctcaaagttg caagaagaat ttccagcttc tattcagttt
    17641 gtttattctt tagtaaaaaa agtcagcaaa agaaaagatg gttatctttt acagacagat
    17701 cagcaaagct attctatcga tcagcttgtt ttagcaagcg gtcactggga aaatgatgag
    17761 acagaagaag aaagagtgtg ggtagactac gcaaagaaaa atgatttact gtatcagctt
    17821 ccttctaatc ctgcagatgt cgatctttcg aagataccgc ctaaagaaaa tgtcttttta
    17881 agaggtctag gcttaagttt ttttgattat gtcggattgt ttacagtatc aagagaaggg
    17941 cgcttcgaga acgaaaaagg aaaacttgtc tatcatccat caggcaagga accgattgta
    18001 tacagtggat caagaagggg gctcccttat taccctcgag gaagaaacca aaagcaaggc
    18061 ggatcaatgg catggccacg gttactaaca aaagaaaacc tggagcaatg gcatcagtcg
    18121 ggatttttga ctggcgaaac attttttgac tatctgaaaa aagacgttga gttgttttat
    18181 tacaagaaaa tcatcgaaga atatcatttg ccgattccaa agaaaaattt tgagcatgat
    18241 tttctctccc tctcttcaga ggagtgtctc ggaaagcatc cagcactaat cccttataga
    18301 tgggattggt cgtatttaga aattcctctt acctatcagg atgagtcctt tgcagaagct
    18361 agccgagcat ttattgaagg gcagataaaa gaagcggaaa aaggcaattg cacaggagca
    18421 tttgcttcaa catttgatgc attaaaagac tggcgtgatc caatccggca ggctattgaa
    18481 tggcacgttt tttctgcaaa ggaatacaaa gaaatactat ggggatggtt tactcatcta
    18541 aatagctttt taacgatcgg tccgccagtc attcggacga aagaattagc agcactgata
    18601 gatgcgggta tttttcattt gttcgaaccg ccatttgata ttcagcaaaa gcacaaccgc
    18661 tatgttattt ctctacaaga taaagaaatc agcagtcgct atttgattga agctcgattg
    18721 ccacaaacaa gcttttctcg ttcaagtaat cctgtcataa aaaatttgaa ggaaaacaaa
    18781 cttgttcgcc ctttttcatt ttcagataat acaggaagtt atcattcaga agctatagat
    18841 gttgagcgag gtacgaatcg cgtgattgat gcttcagggg aaatccagtc tgacttttat
    18901 tgctatggaa tacctgttga aggtgtggat tggctaactg cagcagtagc tagaccttat
    18961 acagatccat ggaatctgag acaggcagat aaaatagcac aacttatatt gactgacaaa
    19021 taaaaatacg catgatgact tatctgttct ttcacaagtc ttcatgcgtg tttttaaata
    19081 attattaacg agaaacgcgt ttgcgcattg cttttttacg gttttcttcg atcattgctt
    19141 ctttttcgtc aattggatcg atcactgttt ttttcacaga agcgaccaaa ccagcaacgg
    19201 cagcggctgt tgccagactt cctactaata ttccagatac aaattttttc attgaatcca
    19261 cctcatttat tatttgttat atctattatg gaacaaaaga gaaaaaaagg ctagttttac
    19321 gtttgtttgt aaacctttgc aaaaaatcct tgaaaatgtt tactaaatgt tttattataa
    19381 atttaccata tgaat
//
LOCUS       DASJSZ010000053        18610 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.053, whole genome shotgun sequence.
ACCESSION   DASJSZ010000053 DASJSZ010000000
VERSION     DASJSZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.053"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(402..968)
                     /locus_tag="U4B03_001772"
     CDS             complement(402..968)
                     /locus_tag="U4B03_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2068622.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(968..1924)
                     /locus_tag="U4B03_001773"
     CDS             complement(968..1924)
                     /locus_tag="U4B03_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEN2068623.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1924..2580)
                     /locus_tag="U4B03_001774"
     CDS             complement(1924..2580)
                     /locus_tag="U4B03_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN2068624.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2950..4968
                     /locus_tag="U4B03_001775"
     CDS             2950..4968
                     /locus_tag="U4B03_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2068625.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5038..5244
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5395..7809
                     /locus_tag="U4B03_001776"
     CDS             5395..7809
                     /locus_tag="U4B03_001776"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEN2068626.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8157..9710
                     /locus_tag="U4B03_001777"
     CDS             8157..9710
                     /locus_tag="U4B03_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN2068627.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10091..11875)
                     /locus_tag="U4B03_001778"
     CDS             complement(10091..11875)
                     /locus_tag="U4B03_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068628.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11890..13611)
                     /locus_tag="U4B03_001779"
     CDS             complement(11890..13611)
                     /locus_tag="U4B03_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068629.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13711..14472
                     /locus_tag="U4B03_001780"
     CDS             13711..14472
                     /locus_tag="U4B03_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2068630.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15439..15707
                     /locus_tag="U4B03_001781"
                     /pseudo
     CDS             15439..15707
                     /locus_tag="U4B03_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16379..>18610
                     /locus_tag="U4B03_001782"
     CDS             16379..>18610
                     /locus_tag="U4B03_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068631.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6116 a         3302 c         3754 g         5438 t
ORIGIN      
        1 ttcttgttgt aaagcgatat ttgaggggca aaaaaggggc ataatttgac tgatattaag
       61 ttagctattg atatatgaat gacatacaaa aatataaaat cttttaaaaa tgcttatata
      121 tcaatgtttt tgccattctt ttatatatct gttatatgtc aaaaaaatgt gttcaatttc
      181 aatttgtatt tgaataaaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacag
      241 ccaatcaaaa acagcttctc atgtttttga tttggttgga cttattaccg cagatataag
      301 cttttgagca ccgtgaataa agaggttatg aaatcaacgc tctaacctga gtattagaga
      361 aaaaaacgca aaacatatca tttatgtttt gcgtttttta tttatctttt tttctcaata
      421 caatataaaa taggcggctg gttcttttgg ttgataaacc gataattcag cacattatat
      481 gtttcctgtg gcagtttgga acaaaaagaa tcaaccatat ccagttcttt ttctccacct
      541 tcatgaccat agtagacgac aaggatcaat cgtccgcgag gcacaagtcg tttcaatatt
      601 tcttccatag ctgtgcgtgt agtctctggc atagtgatga ttgctttatc actttttggg
      661 agatacccta gattgaaaat ccctgctttg agattttgtt gttcagctat cacttctcct
      721 aacgtttcgt ggccttgtag aaacaatgag acacgttctt ctaactgacg ctgttctagc
      781 ctttcacgag tagcatcgat tgcttgttgc tggatatcaa aactatagac atgaccagtt
      841 tttccgatgt gttctgctag aaaaaccgta tcgtggccat tgcccatcgt tgcatctacg
      901 acgtgatccc ctggttgcaa gatttcttgc agtaatgtat ggctgaaatg caaagcagtt
      961 tgcaacatca gacgctcacc tttttccgta catcatattt tccttggaag ctatctctgc
     1021 gaagaagttc ttcatcaatt gcattcaaga cttcccattt tttcaagctc cacattggtc
     1081 caatcaagga atcccacggc gcatcccctg tcaaacgatg gatgatgatc tcttttggta
     1141 tcatttccag ctggtcgcaa atgacattga catagtcagg acggctcatt aattgaaggc
     1201 gaccttcatg atagtcccgc aacattctcg ttttgcgcat cagatgcatc aaatggagct
     1261 tgattccttg gatatctgaa tctaaaatgg tgcgtctgac attttctttc atcatttcta
     1321 aactttcgcc tggtaatccg ttgatcaaat gcgtacaaac acggatatta tgtttgcgta
     1381 atttagccac gccatctaaa taagtttgat aatcatgtgc tcggttgatc agttttgaag
     1441 tttcttcaaa agttgtttgc aatccaagtt ctacccataa atacatccgc tcgttcagtt
     1501 cagcaaggta atcaactact tcatctggca gacaatccgg tcgtgtaccg actgataatc
     1561 ctacgacacc tggtaaattg actacttgct caaatcgttc tttgattatt tcaactggcg
     1621 catgggtgtt tgtaaaattt tggaaataaa cgatgtattg ttggacatga ggccattttt
     1681 gatgcatcat gtcgatttct ttctggaatt ggattggaag agggtcttca ggtgcaacga
     1741 tcatgtctcc cgaaccagat acactgcaaa aagtacaacc gccatgggca acagtcccat
     1801 ctcgattcgg acagtcaaat cctccatcga tcggcacctt gaaaattttc ccgccaaact
     1861 catttcgtaa agcgtaattc caagaatgat atcttttatt tccttctgaa taaggaaaag
     1921 tcattaaaag tccttctttc tgtaagagtt atctatttgt tttctttttt ctctatacac
     1981 tggataagtc aggcacagcc aagaaagact gagtaagtaa ccgccgatga tatcagtagg
     2041 aaaatgaacg cccagataaa tgcggcttat cccgacactt actataataa acatcatcag
     2101 caattgaagc aatgtgcgta aggttctttt ttgaacaaaa gctggaagca gaaatagtaa
     2161 agaaccgtaa agaatcatac tgcctgctgc atgtccacta gggaaactgt aacttgtttc
     2221 aactactaag tgcgccaatg aaggacgctc tcgcatgaaa aatagtttga tcaccgggtt
     2281 gatgattcca gcaattacaa taaatccaga aacgaaccaa aatgtttctg cccttttttt
     2341 cttataccac atccataaag ccgcacaagc aaataaaatg atgacggtaa gaggattacc
     2401 gaatttagtc ataaagagtt ggtagctgtt cagatcagga taagaagctc tgacaatgcc
     2461 tgtaaaccac tgatcgaaac cagaaagcca cgacagatgg aactttacga tccagcccaa
     2521 aatgaaaaac aggacaagaa agaagctacc agccacctga aatgatgttt tatatttcat
     2581 acaattcctc ctttgaagca atgacttatt atagcatttt tggtccagcg aaaaaaacgg
     2641 taaagagata aaaccaatgt atgattcctc tttgaataac caatattctt ataaaaaaaa
     2701 taactgtaat cggaaagaca ttttttggta atgcaaatga aatgttaggg aaataaaatc
     2761 agtgtttttc gaaatccttt taaacaagaa cgtagacgta ggaagaactt gaaaatgaac
     2821 agaagttgtt cattacggta aattacaata tgattacaaa aaacaacaaa gctcttaaat
     2881 aacttttaat aaatattaag agaagttata attattaata atagataaat tgaagttgga
     2941 ggatagaaga tggaaaacat tgcaagaaaa gaacggagac gtttagaaca gaccaaacgt
     3001 tttcgaaaag caaaacgagg ggcagcaatc gtgggaactg cgatggtagg ctgttctgtc
     3061 gctgccccat tagtacagcc ggttcctgtt caagcggatg aaacacctgt tcagttcggc
     3121 gcaagaatca atacagcggc atttatcaca gaaattgcta cttatgctca accaatcgct
     3181 caagccaacg atctatatgc atcagtgatg atcgcacaag cagttgtaga aagcggttgg
     3241 ggcagtagtg cgttatctca agcaccttat tataatcttt ttgggataaa aggcagctat
     3301 caaggacaaa ccgtctacat ggatacgctt gaatttttaa ataatcaatg ggtaaataaa
     3361 aaggagcctt tccgacaata tccttctttt gctgaatctt tcaatgacaa tgcctatgta
     3421 cttagaaata cttcatttgg caacggttat tactatgccg gtacttggaa gagcaacacg
     3481 aaatcctata cagatgcgac agcttgtttg acagggcgtt atgcgactga tcctggttac
     3541 gcaggcaaat taaataatat catcgcaact tacggattga cccaatatga tacaccagct
     3601 agcggaaacg caggcggtgg agcgactaca ggtggcaata atactggaag tgcaggtagt
     3661 actggcggtt caagcaattc tggtaataca aactcaggtt cagcaacttc gaccacttat
     3721 actgtccaat ctggtgattc tgtttgggga atctcaaata aattcggtat cacaatggat
     3781 caattgatcc aatggaacaa catccaaaac aatttcattt atccaggaca aaaactgaca
     3841 atcaaaggta gccaggaaaa tggctcttcg acgaataatt ccggtaacaa taccaattct
     3901 tcaggaaatg ctggtacatc aaacggagga caaacaactg gtgcgaaata cactgttcaa
     3961 tctggtgatt ctgtttggaa gatctcaaat gatcacggaa ttacgatggc tcaattgatt
     4021 gaatggaaca atatcaagaa caatttcgtt tatccaggac aacagctaat cgtatcaaat
     4081 ggcggttctt ctaatgctgg agcaaatact ggctcaaata ctgggaatac aagtggctct
     4141 agttcgtcca gctcaagttc ttctaataca gcttcaggaa cgaaatatac agttaaagcc
     4201 ggagactctg tttggagcgt ctcaaataaa tacggaatta cgatggctca gctaatccaa
     4261 tggaacaata tccaaaataa ctttatctac ccaggacaac aactgactgt ttcaaacagt
     4321 ggatcaacaa acagcacgtc aggtaataca ggttcgtcaa ccaatacaaa taacagcagt
     4381 tcagttaata ctggcagcag ttcgtctcaa ggcacttata ccgtgaaagc aggtgaatct
     4441 gtttggagcg tggcaaataa aaacggcatc acgatggatc agttaatcga atggaataat
     4501 atcaaaaaca acttcattta tccaggacag caattgatcg ttaaaggtgg aacttcggca
     4561 aatacaaata caggttcaac tacttctgca gcgaaaccaa atacaccgaa tacgtctgct
     4621 acgacaagta catcatccac tggtaatacg atgtataccg tgaaagcggg cgagtcagtt
     4681 tggagcgtag cgaacaaaca tcatatcacg atggatcagt taatcgaatg gaataatatc
     4741 aaaaataatt tcatctatcc aggacagcaa gtgattgtga agaaaggttc atctcaatca
     4801 acaaaccaac aatcagttac tggttcgaaa acctatactg tgaaatctgg agaatctgtt
     4861 tggagtgttg ctgacagtca tggaattaca atggctcaac taatcgaatg gaacaatatt
     4921 aagaacaact ttatttatcc aggtcaaacg ttgattgtag ctaaataaaa tagctgttcg
     4981 aagaaataac gattgaattc cacaaaaata tttgttacta taaggctact taaatatgac
     5041 aacacttgaa aaggaagtag taaaaagact gaagtttcag agagcgcatg gctggtgaga
     5101 atgtgcacgg atgcttttga actcgccttt gagttgccct tgctgaatac ccgagtaagc
     5161 aagcggcggt gacggccgtt acaacgttcg aggccttttc actaaggtaa aattaggtgg
     5221 taccgcgtag attacgtcct ataaaaagag caatcttttt ataggatttt tttatttctt
     5281 ttgtgaagtt tgagaaaaca aagcaaaagg aaataaaaat aaacagattc tgccggtttt
     5341 ttcgttatat ttataataga aacaagtgtt ttcaatagaa caggaggaca gcaaatgagt
     5401 tacaatcata aagaaattga gaaaaaatgg caaaaatact gggcaaaaac gaatactttc
     5461 aacacccatg atgatcctga aaagccaaaa ttttacgcac tggatatgtt tccctatcca
     5521 tccggtcaag ggctacatgt agggcatcct gaaggttata cggcaacgga tatcctttca
     5581 cgtgtgaaaa gaagtcaagg atacaacgtg ctgcatccaa tggggtggga tgcattcggt
     5641 ctgcctgctg aacaatatgc gctagataca ggaaacgatc cagctgagtt tacccaaaaa
     5701 aatatcgaaa cattccgacg ccaaatcaat tctttaggat ttagttatga ctggaatcgc
     5761 gaagtgaata caactgatcc ggagtattat aaatggacac aatggatctt tacgaagctt
     5821 tatgaaaaag gtctagctta tgaagcagaa gtagcggtaa actgggtacc tgaattagga
     5881 acagttattt caaatgaaga agtgatcgat gggaaaagtg aacgtggtgg ctatgatgta
     5941 gtcagaaaac caatgcgcca atggatgctg aaaatcactg cttatgctga tcgcttattg
     6001 gatgatctag aattagttga ttggccagaa agcattaaag aaatgcaaag aaattggatc
     6061 ggtcgttcag taggagcaaa tattgaattt aaagtggctg gtacggataa atcctatacc
     6121 gtatttacga cacgaccaga tactctattc ggtgcaactt actcggttct tgcacctgaa
     6181 ctagatcttg ttagagaaat tacgacccct gaacaaaaag ctgctgtaga agcttatatc
     6241 gaagagacag ctaaaaaatc agatctaaag agaacggatc ttgcaaaaga aaaaacagga
     6301 gtatttactg gtgcttatgc aatcaatcca gttaatggca aggaaatccc aatctggatc
     6361 gccgattatg tattatcttc atacggcaca ggagccatca tggctgttcc agcacatgat
     6421 gaacgtgact ttgaattcgc tcaaactttc ggactagaaa ttcttccagt tatcgaaggt
     6481 ggagatgttg aaaaagctgc ttacacagaa gatggtacgc acatcaactc tgaatttcta
     6541 gacggcatga acaaacaaga agcaatcgat aaaatgaatg aatggctaga agaaaatggc
     6601 gtagggaaaa aagaagtcag ctatcgctta cgtgactggc tattctcccg tcaacgttac
     6661 tggggtgaac caatcccaat catccattgg gaggacggaa cagtcacagc tgtcccagaa
     6721 gaagagctac cattacggtt gcctaagaca aaaaacatca agccaagcgg tactggagaa
     6781 tctccattag caaatatcga ggagtgggtc aatgtcgttg atcctgtaac aggtaaaaaa
     6841 ggccgtcgcg aaacgaatac aatgccacaa tgggcaggaa gctcatggta ttacctgcgt
     6901 tatatcgacc ctcataacaa aaaagaacta gcagattatg aaaagttgaa gcgttggcta
     6961 ccagttgaca tctacattgg tggagcagaa catgcggtac ttcatttact atatgcacga
     7021 ttctggcata aattcttata cgatatcggt gttgttccta caaaagaacc attccaaaaa
     7081 ctatacaatc aaggaatgat cttaggcgaa aacaacgaaa aaatgtcaaa atctcgtggt
     7141 aatgtagtca atcctgatga tgtggtcgaa acttacggtg cagatacact gcgcatgtac
     7201 gaaatgttca tggggccatt agatgcttcg attgcttgga gcgaaaatgg cttagaagga
     7261 agccgtaaat tcttggatcg tgtatggcgt ttgatcgttg atgagaacaa taaaatgcgt
     7321 gaccgtatca ctacattgaa tgatggcaag cttgataaag tgtatcatca aacagtgaaa
     7381 aaagtgacag aagactatga aaatcttcat ttcaatacag caatctccca attgatggtc
     7441 tttatcaatg aagcctataa agtggacgct ctgccttatg aatatatcga aggatttgtt
     7501 caactactcg caccgattgc tcctcacatg ggtgaagaac tatgggcaat ccttggaaat
     7561 gatggtggta tttcttacgc accatggcca acatacgatg aagcagcact tgttgaagac
     7621 gaagtagaag ttgttttcca agtaaacggt aaagttcgtg cgaaaagcaa tgttccccga
     7681 gacttaggaa aagatgagct agaaaaagca gcattagcca atgaaatagt gcaagaatat
     7741 attgaaggca aaacagtcag aaaagttatt gcggtaccaa acaagttagt caatatcgtt
     7801 gctaattaat tggctctttg tcaataagga ctgatgagtt gtgcaaaatc aaatctgggc
     7861 cagaatgaac ttcattctgt ctcagatttt ttgttatttc aaattgatta attgattgat
     7921 caaaaaggtt ttgattttca tgttctcaaa cacgttcaat gtctttttct tctgaatggt
     7981 cttgtaagag atctctgtaa tcatcaaatt tttgtctatt aatcttagta ttttttgatg
     8041 tataaactac ctgaaattga gagtacatac caaattcata aattttttat tagtgggact
     8101 agtgattgta tggattttga cgcataatga atgaagtatg agaaaaggag agacaaatgg
     8161 atcaagaact atttattatc gaagtattat cagctaaaca aaaaattatc gagaaagcga
     8221 aaaaaagagc gaaaagaata atgttcaagc ttttaagctt agtatttttc tctggtttgc
     8281 tttttttcgg agctgtatcg ttcctatatc gccatatgag tgcagtagtc cgttcagctg
     8341 aagggatgca aaaattaaat aatttgtatc acttttcgtg gggagttggc ggaataacaa
     8401 ttgttgtgct tattgttttt cttctactta tttggctccg ttaccatcaa ttgtcgcgtc
     8461 atttcgatca atggcttagt caaagagcac aacagttttt acataataaa ccaacttctc
     8521 aagatgatga ctatctttat tttgagaaga cttcgacgag tcaagctttt cggatacaaa
     8581 aaagtgattg ccagttattg ctgagatcac ttgatgattt acctatctac ttaggcgaaa
     8641 gcaaatcaaa attcggaatg aacacattcc atattttttt actagaaaac gaacccagat
     8701 gggaaaaaag ccagtcttcg cctggtggat acctgacaac taagatgagt acattagtca
     8761 ctggtttttt cctcattctt ttagctggaa caatctatta tgggagatac actcagccaa
     8821 aaatgtcttc tcatataaat gctgcttctc acatgacaat gaactcaact aacgatacgc
     8881 atatacaaaa gacaaatgaa gggttgttag ctgatcctaa gcaaaaggta aatttgacaa
     8941 cacaaaagat aaatgatctc aaacttgata cagagacgca ctctctctat atgacaacag
     9001 atagtggtgc taattgggta tttgtgccga ttgattcgca atggttgcgt tttggcgaat
     9061 acaccctgac aacggggctg attcctgaag gttattggat ggacaacaca tatgatattt
     9121 ccgccgattt ctcatggttt atctattctt ctggggatga tgtacgtctg ctgacttcaa
     9181 gagataatgg taaaacatgg caaacgtcta ttatcaccca atcagttcag ccgatacgtt
     9241 atcgaaaagc ggtattttat ggcaacgatg gtctatttat ctgtacatca gccagtggga
     9301 tgtctgcaga aactttgtac atttacacta ctatggatgg cggaatcact tggataaaaa
     9361 gtggaagtac aatgatcaat cagccagtac aaaatgtcag cttcgttaca acttctttag
     9421 gttttgtttc gacacgtgaa agtttgtttt atacaaacaa cggaggacgc tcattcaaag
     9481 aatcagttct gtcgattccg gaaaactatc agacaggggg gatagatctt tttcaatcac
     9541 caaatgaagt tactcaaata agcgcgaata cgttagagac aaaattttat ttgttgaaaa
     9601 aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt ccgttcaact gataacgggg
     9661 aaacatggca attcgttcag caactatcgc aagtagaaca aggagattaa agaagagaga
     9721 ttgtgagcct gacgttcagc accgagtcac aagaaacaaa ttcaaaaaac agcttctcat
     9781 atttgttgaa tagtgtaact cgtgattcgg agttgacaag agcaacaaga agcgattttg
     9841 aagtatagct tctcgtattt tacaagatcg tgtcttattg ccgataggtt gtctcttgaa
     9901 caccgtttat tcgtaaaaag ggattgtgac aagtcttttg tcactactta ccgaacgaaa
     9961 ctgaaggagc tatccaaaaa gtatcattag tattattctt tgtgtttcta gtggcaccac
    10021 tagtacattt aaaaaagcta tcgatcatgg actaatcata catgatcgat agctttttag
    10081 ataatgtttg ttacgctatc tgaaattgac tattataaag actggcataa agcgttggtg
    10141 attggagtag ttcagaatgt gttccttttt cgacgatatc gccattcttc attacaagga
    10201 tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat cacaaaactt gtcctatctt
    10261 ttgtcacagc ttccattgct tgctgaatca atgcttctgt tcgtgtatct acgctagaag
    10321 ttgcttcatc taaaataacg acaggaggat tcgctagaat gactcgagca attgtcaata
    10381 attgttgctg tccttgagat aacgtgccgt tttcgctaga aatgatcgta tcatatccgt
    10441 ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc agccgcttcg ataatttgtt
    10501 ctcttgtggc atccattttc ccgtaggcga tattatcagc taccgttcct tcaaacagcc
    10561 aggtattttg taaaaccatc ccaaaagaac gacgtagttc tccacgtgaa agcttggtaa
    10621 tatcaagacc gtcgaattcg atctttcctt tattcagttc ataaaaacgc atcagcagat
    10681 tgactaaagt cgtttttcct gcaccagttg gaccaacgat cgctaccgtt tgttttggct
    10741 ttactgaaaa atcgacttga ttcatcaata ttttatctga tgtgtaacca aactgtacat
    10801 ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct tgcatctgga aggtctggaa
    10861 tttcatctgt ttcatctaat atctcaaata tcctatcgat tgctgccatt gcggattgga
    10921 tcgagttgat cacataagaa gcggtagaaa ttggttcaga aatctgattg atgtactgaa
    10981 gataagcttg taagaagccg atcgtcacac cacctgatag aacaagcata gcaccgaata
    11041 ccgcacttat gatgaaggcg agttgattaa tgaaacgaat ggccggatag atagcacagt
    11101 tcataaattg agcacgttta aaagcgttgt attgcttttt atttacttcg tgtatagcag
    11161 cttctgcatg agcttgctga ttgaatgttt tgaccactag attaccagct aagatttctt
    11221 ccattcgatt atttaaaaca cccagttcat actgattctg ttcagaaatc tgtttgtttt
    11281 tgttcgcaat cttagttgta acaattgaac tgccaacgat cagcaataag acgagcaaag
    11341 taagcttgct ttcgatataa aacagcatca gaccagcagc agctattgtt acgacagaag
    11401 taaaaaattg attgatacct gtcaataaca cttgagaaag ttgattgaga ccggtagttg
    11461 tccgactaat gatgtcacct acttgatgat tgtcaaaaaa cgacatcggt aatgttttga
    11521 atttttttgt gatttctttt cgcaccctca atgtgatctt ttcgcttaac gctgccattg
    11581 tccgttcctg aataaatgaa gtgatgctgc ttaagactga acagccgatc agcaataaga
    11641 cagggaacaa caatgcttgt ttgatatcac tgaaagaaag ctgtccaata cctgtttttc
    11701 ttatttctga taagagatca tcgatcccga ttcccatgat caagggcatg gctacgacta
    11761 ataggttccc gaataaactg catagaaaca atccgtaaaa atatttatgt tcaggtttga
    11821 tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt ttttttcttt tccatcgtat
    11881 cccttctttc tatattcctt gtgattttgc aaactcctga taagtttggc aattagacag
    11941 tagttcttca tgagtacctt gtccaacgat ttttccttca tctaaaacaa tgatgttatc
    12001 tgcttgttgg attgtactga ggcgttgagc aacaatcacg agagttttct ccgacatgtg
    12061 tttatgaagg gctgctctta atgctgcatc tgttttatag tctaaagcgg agaaactatc
    12121 atcgaataca tagatctctg ctggtttgat caacgctcga gcaatacata tccgctgttt
    12181 ctggccccct gaataattag aaccgccttg agcaacaaag ctgtccaatc cgtcaggaag
    12241 ggtgtccact agttcttcta attgagcgat cttgatcgct ttagacaact cttctgttga
    12301 ggcatattca tttcccatct gcaagttgct taaaattgtc ccgctgaata agaaagcctt
    12361 ctgagggaca taactgatgt gggagcggat ctgttcttga gtcatttgag taagaggaat
    12421 accaccaagg gttacttttc ctatcgtagg gtcactaagt ctcaagagaa gtttcgccaa
    12481 actacttttt ccagatccgg tcccgccaac gatcgctgtt gtttttcctt taggtattgt
    12541 gaaatcaata tcttctagaa ctggatcaga gatttcagta tatttaaatg tgacatgttc
    12601 aaaagcagct gcatttgtct gatcaaaggt tggttcagct actgttttga tcgtctcttc
    12661 ctcaatttcg atctctgctt gcagtatttc ttctatccgt cgtaatgagg caaacgattt
    12721 agggataatg accaatacca tagctgcgat catcaagtaa gcaagtgtca gcatagagta
    12781 ttcgactaca gcagtcacga caccgatttg taatgctcct tctgctacaa gatttccgcc
    12841 aaaccacaaa atagaagcat aaacgattcc catgagcaaa aaagcaattg gagtgaccca
    12901 agcaaatatt cggttcactt tgatcatttg attagcatag tcagcaaagc tcttattcgt
    12961 tcgcttttct tcatatgatt gattatcaaa ggcacggatc atattaatcc cagtaaagaa
    13021 ttctctcaat gtgatggtga tccgatccat ttttggctgg atactcaaag ataaaggtgc
    13081 ccctcgtttc atcagtcgat aaacaatcca tacataaaaa acaacagaga tcaatgtaac
    13141 ccatgttagt aatagagaat aacgaaacat catgagaatg gtgaaaacgc aaataaccgg
    13201 tgctggtaag atcatttggc aaaataaaac aatcatttgc tggacattat cgacatcatt
    13261 cgtcattctt gtcaaaagag atccagaact aaaacgactg atatccttca ttgacagctg
    13321 ttggaattta cggaaatatt tctcccgcgt ttggaatcca aacctagcgg aagccaatgc
    13381 tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga aacgctacaa gcagcatttc
    13441 aactccaatt ttttttataa ctgaggcttc gccacttgct atacctgtgt cgatcagctt
    13501 tgctactaat tttggtacac ctaatgtacc ggcgatttct aaacagatca tcacaaaggt
    13561 aaggagagcg agtcctttgt ttttctgtaa gaatgttttt aagatagaca tattattcct
    13621 ccttttttcc tgatactttt cgattctata ccttcaagta acttgaaggt caatagaaaa
    13681 aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa tgtatacgat tggccaagta
    13741 tcggaactat ttgatttgcc aaaatcaacg atccgctatt gggatgaaca aggattaatc
    13801 cgttcaagta gacaagaaga gaatgattat cgattgtttg atatcgatga tatttttatg
    13861 ctttatgata tcgtttttta tagaaaatta gatataccga tcaaacagat gaagaattta
    13921 tatggaaaaa cattgacgga gttatatgag acacttgatg aaacagaaag aagaatccat
    13981 aaagaactgg tggtgatgaa acaaaaacaa aaagagataa gggagcaaaa aaaacagttg
    14041 aaattgatga tcgatacaaa cgaggaagaa tttcctgtag aagagatccc ttttgactgt
    14101 atgatctcaa cggagtttga agatattgtg gaaatcaaaa aatttctacc taactattca
    14161 agctttggga tgatgagtat gccagcttcc agttcacaga cgatgtatgg attttttatc
    14221 gaccctagtg aagtacattt atttactcaa gacgtgattt gggagaaaaa agatacggct
    14281 gtttatcgtc gattcttatt gaaatccgag atgaaccatg cggaacgtaa taatatttta
    14341 gaaatcagag aaagaatgta tgaaaaagga tggaaaacag gagaagtgat cggccagtat
    14401 ctattgacga atacggatga aaacaatatc agaactgaat attatcatgc atggatagaa
    14461 atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa tgatggttgt tttagttata
    14521 aagaaaaatt ttctggttcg attacgggtt taatgtgggg gattattagt cgtggataga
    14581 tagaatttta gttttttaga ggaaatatgt ctttaaaaat ggaatggttg caattcttag
    14641 cgatatatac gatgtaattg tcctcttaaa aaagtatgga aaaataattg tattgggtgt
    14701 tatgtataat tcatggtgaa ctgagactaa tgagaagaaa aattcatatt gttatgagtg
    14761 agattgtatc agatgagaat gataaattaa ataggtgcat aaaagcatta cacttaaaat
    14821 acattgttgc ttacattatt acaatatgtt aataaggcaa aaataaatag atggattgaa
    14881 taaacatatt ttttaatcaa atagtcccta ctgtgacttt cctcagctat cggtgatact
    14941 accgatagca tttttttgct tataaaataa ttataagtat ttagagattt ttcttctaac
    15001 attcttcaaa caagaaaaat cacaaaggtt attgttattt ttatatattc tttttaagaa
    15061 tgattgtttt tttatagaaa ttttaaaaaa taaaacacgt tattgatgga tagttagttt
    15121 gttatttggt aatattatag aagttgactg gtagtgattt atttttcgtc gttttataaa
    15181 aatgttattt tttggaaaaa ttcacaaata tgtaacgaat gtttttctgg aatttgctgt
    15241 gttaaaaagc agtatgacta atataaatac tattgtcatt tatgcaattt tttatggata
    15301 gtaattttag aggggaatca atgaaggaag aatagaaggg aaaaatagtt gcaaatacgc
    15361 cagttagcaa aaatagcaat acatcttcaa gctttaaaat tcgagagaat catcttggta
    15421 gtggaatcaa ctacaacaaa aggattaaac caattaatag atagaattac caatggaaaa
    15481 atagaaaagg tagttattct atacaaaagt tgtttaattc gcttcggtta tgaacttagt
    15541 gaaacccttt gcaatatatg gtacagaata tagaaattat tgacattaca gaaaatgatg
    15601 aagatttaat acagattgtc actatttgta gatacctttt acaaggaaac cgagccaata
    15661 aagctaagaa gatgattaag gagttgatgg aagacgatac aagttaagaa agtgaaacta
    15721 cgcccaaata aagagcaaaa agagtaattg gacgtggaaa atgcaagaaa tgaaccatcg
    15781 attcggtgga cagtttatta gtaatgacgg tttgagaaca tccatgacga gaatgaagga
    15841 acatcctaaa tgcgtttagt tgaaccaagt gccaaataat atggccaaat aagcgatcag
    15901 ttatcgatga atcccaagta ttctaaacta tatattcatt ttgatgctaa gcattgatat
    15961 atttttttgg gattgatgaa ggttaaccaa tacagggact cactgatgaa attctagagt
    16021 ctgaccttgg tatcaacgat ttagtgattg tttctaatgg aaaagtataa aagtatataa
    16081 gtacattaac aaaataacta ttagggttac gctccagctt gtgcaaggca ctggcaggag
    16141 gaatcgtgaa atcataatta tggttaagcc acacggacaa ctaaaagtca tacaaagcta
    16201 tcgaatcaat caacagaaga caatcggcaa tcattagctt tgtttacagc ttacattgca
    16261 acgcttgagt tgaaccgaaa atacgaggga ggtaacgcag ccattttgag ttggggaatc
    16321 tttttagaaa attatcgggt accttgttgt ttagtcaaaa ttataagggg gagtaatagt
    16381 gaggaacaaa tttcataaac tgttaagtat gtttctagtt atgcttattg tgatcaatca
    16441 atttgggaac ataacggttc tagctgagtc tttagcagaa cagccagagc gttcgctgac
    16501 gatcgaagtt gatcaagagg aggctgcttt gggtgagcag ttccagttgc gagtcagagg
    16561 aacaccagac ctgctaaatg cattggtcat tgataagagt gaggatttga ttgaagggga
    16621 agtgaagcaa ctcaatgatt acgaacgtct gattacttat tcttcccagc attcaggtaa
    16681 atacgaattg accgcaagaa gcggtgatat tcaatcaaat acggtctcaa tagaaatcag
    16741 tgtaccagaa gaaagcacaa gtgagtcttt taatgtacca gaagaaagca caagtgaatc
    16801 ttttaatata ccagaagaaa gcttaagtga atcttttaat gaagccagat catctgagcc
    16861 atctataacg caggaatctc agattgaaag acaagaggag ctcttgaatt atgacatcgg
    16921 catctctgct gagcgcccca ctttgccgag caggcataca caaacgttaa agggatacat
    16981 tcaaggaggc gctggcgata ttcttgaaga atatggggag atggtcgtac gagttcctag
    17041 agcatcgatt ggcttaattg agttcattag agtggaggtc gctgatgatt cacctttaaa
    17101 gccactggca ttttaccaag aaggcacaga cgttgttttt ccatttacct ttgattatga
    17161 aggattaaat gaagccgctc agtcgtatac attcagtatc acgtttgaag ttgctttctt
    17221 tcaatcaggc ggtactgagc caaataatga ctttcaagtg agtcttactt atgctgagcg
    17281 agcagcaaac acggaatttt caattacgcc tattccttca tcaagaccca ttttttcgaa
    17341 attatactat ggctctagag aaatcgacag ccaaggaaat cttgggaatc tgctgattga
    17401 cgggcaagac gtgaccaaca gtaataaatt tattttgcag gtaaattatg aaggatcacg
    17461 aaaagacggt gtagtggtta ccgatgtact accgcaagga acaactttgg caccgaatcc
    17521 gactacatgg agttccactg gaaatagaga acctatttca ggtggggttc ggatcgcaga
    17581 agtaacgaat ccaggtgaag caaacgttag aactcattat gtttccgagg aatttgtgga
    17641 caaagtatct tttaataagg aaacgaatac acttgttgtg gactttggta atctagcaga
    17701 gggtcaatcg tattttattg aatatgctct tgaagtgacc gatgtcgact tgttcaatga
    17761 aggaagacaa gaggatgggt tttctcggaa tcgagcgagt atgacctaca ataatcaggt
    17821 aatgagccga gactaccgct tgagaatccc tgaaagtaat cttccggcaa tctctttaag
    17881 aaaaagtgtg gacaaaaatg ttatcaatct caatgaaaat gaactaacgt atactctgca
    17941 tgtctcgatg ttttctggaa caagaaatac gcttgttatt gaagatgttt tggaagaagg
    18001 catggaattt gtgtcatttc tatcagatat tccctctttc attgatgccg aagtcaataa
    18061 tggaatagtt cgtttcagtg cgaataatac gattggtggt ccgacaagtt ttaccctctc
    18121 ttttaaggtc aatgtagagg cgtatgcggt cggcgatcaa attttaaact ttgcaacgct
    18181 tgtgaatagt gacaatcgat tgaatacgag tactgtaaga acaaggaaaa tcgatgggcg
    18241 tattaaagta attaaaactg atgagaatgg ccaaaggctg gcaggagcaa aatttgaaat
    18301 tttatcaagc gattcacaag tgatacaaga aggtataacg gcagaaaacg gagaattttt
    18361 aagtgaaccg cttcctgtag gtagctatca agtccgagaa acagaagcac cggaagggta
    18421 tcttttagat gcgacggtac atgatgtttt tataaatgaa tactccatag aagaagtgac
    18481 gttaaccatt gaaaacatcc ttgatacggg tggtgttgaa ctaattaaac atgctgaagg
    18541 cagtggtgaa gtccttcaag gagctgtttt tgagttacag aaccgagaag gagaaacgct
    18601 acaaaccggt
//
LOCUS       DASJSZ010000054        18553 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.054, whole genome shotgun sequence.
ACCESSION   DASJSZ010000054 DASJSZ010000000
VERSION     DASJSZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.054"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            62..937
                     /locus_tag="U4B03_001783"
     CDS             62..937
                     /locus_tag="U4B03_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068632.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1082..1234)
                     /locus_tag="U4B03_001784"
                     /pseudo
     CDS             complement(1082..1234)
                     /locus_tag="U4B03_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1502..2833)
                     /locus_tag="U4B03_001785"
     CDS             complement(1502..2833)
                     /locus_tag="U4B03_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2068633.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(2982..3695)
                     /locus_tag="U4B03_001786"
     CDS             complement(2982..3695)
                     /locus_tag="U4B03_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2068634.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            3870..5294
                     /locus_tag="U4B03_001787"
     CDS             3870..5294
                     /locus_tag="U4B03_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2068635.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5593..6300
                     /locus_tag="U4B03_001788"
     CDS             5593..6300
                     /locus_tag="U4B03_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN2068636.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6287..6610
                     /locus_tag="U4B03_001789"
     CDS             6287..6610
                     /locus_tag="U4B03_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN2068637.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6699..7736)
                     /locus_tag="U4B03_001790"
     CDS             complement(6699..7736)
                     /locus_tag="U4B03_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2068638.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8057..9040)
                     /locus_tag="U4B03_001791"
     CDS             complement(8057..9040)
                     /locus_tag="U4B03_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEN2068639.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9455..10225)
                     /locus_tag="U4B03_001792"
     CDS             complement(9455..10225)
                     /locus_tag="U4B03_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068640.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10218..11153)
                     /locus_tag="U4B03_001793"
     CDS             complement(10218..11153)
                     /locus_tag="U4B03_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068641.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11469..13118)
                     /locus_tag="U4B03_001794"
     CDS             complement(11469..13118)
                     /locus_tag="U4B03_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN2068642.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13235..15838)
                     /locus_tag="U4B03_001795"
     CDS             complement(13235..15838)
                     /locus_tag="U4B03_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN2068643.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15850..16899)
                     /locus_tag="U4B03_001796"
     CDS             complement(15850..16899)
                     /locus_tag="U4B03_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEN2068644.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(16925..17239)
                     /locus_tag="U4B03_001797"
     CDS             complement(16925..17239)
                     /locus_tag="U4B03_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEN2068645.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17255..17701)
                     /locus_tag="U4B03_001798"
     CDS             complement(17255..17701)
                     /locus_tag="U4B03_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2068646.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17702..>18553)
                     /locus_tag="U4B03_001799"
     CDS             complement(17702..>18553)
                     /locus_tag="U4B03_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2068647.1"
                     /translation="KYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVTLHIQSLLDR
                     YKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRKVTDELVIST
                     IPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCFFTHQIKDNQ
                     VNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFIKKPVICVYV
                     NPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLKTSSSFEKLV
                     YILKEGL"
BASE COUNT         5666 a         3746 c         3047 g         6094 t
ORIGIN      
        1 ttttttctta aacgtttctt tatactaaat ataaataagt gtttcatgaa aggagaacta
       61 gatgaataat tggcttgagt attttcctga aaacgttttg gaacgcggtt acagctatca
      121 tctgcacggt tttgtccgtc atttgaacta tacaagcaag tacttgtccg caacagtttc
      181 tggcacggaa gattataagg tagtgatcac ttgggatgaa aagaccaaca tgacttgtga
      241 ttgtctatat gccatagaag gaaaaaaatg caaacacatg gctgctgtct tattcgctta
      301 cgaagaacgt ccaataaaaa aatccaacta ctcactttcc gaactctcgt cccttgtttc
      361 ctccgctagt tcctctcttg tacgggagct tttaacagag attcttatag aacatccgca
      421 atttatcgaa cgcttcaaag ttaagatgcc atttcatgca ataaattatt cagataaatt
      481 aacaactatc atccataaat acgatcatat tatcaagaaa aacaaaaaca ggaaaacagc
      541 gaagttcatc atggagatgc gaaagttcat ccaagaagca gttgaatcgc tcattcagca
      601 aaatgcctat cttcccgcat tcgaattgat caatgaagtg attgctactc tcgaaacatt
      661 ctattgggag cctgaggatg aacgaaccct tcttctgata gaggactgtt attatttatg
      721 gaaagaatta ttagcagaag cacctcatgc agaaaaaagg caaatgttct cttggttcgt
      781 ctgccaagtc gatcatactg acgcctctta ttcaaaacgc tactccataa aaatcctgaa
      841 agaagacttt cgggaaaaag aattttctaa ccaaaagaaa aaaatagata aaaaaactaa
      901 agaaacggta aagaaagacg atttcaatga aaaatagcaa gtaaacgata acaaccatcc
      961 taaatccaca agaaatacag cagaccttgc tttaaaaaca cgaatcatgc aaaggatgaa
     1021 gtaaagaaga cttaacgcgt acccaatatt agtcatttga ctattaggta cgcgttttgt
     1081 tttataagtc atgacaaatt tccttcgttg ctgggtagat tttccggcat tgttgataga
     1141 ttttatgata cttttctaca ttttgtaaga gaggttccac tgcatctgta tattggataa
     1201 aattctgaca acaactttca ggtgtgtcga accactctaa tctttaaaga taaatattaa
     1261 aataacgatt aataatatta acagtcttta tttttcacca ccaaaaacct atctttgcat
     1321 atactaaata gacgtatata cacagatagg tttgtcttgt gaaaaataat gattatcctt
     1381 accaaaaaat gtttgttttt ctacatgttc gtacttatca aaaaatatga tccttattga
     1441 aattactggc tatttatatt ccaacgactt cttgtgttaa tttttgttat ctcatagtaa
     1501 attatgtttc attttcactt aatttagttg tcacgtgttc gtcaattcgt aaaaacggta
     1561 aatatataaa cacacttgcg gctataatta atatttgtaa aacagctgct cgccagtctc
     1621 cggctgttgc taaaaatccc gatataactg gtggcgtagt ccaaggaatt tgcactacta
     1681 catgattaat taatcccaca gcagttgcaa aatatgctac tgtcaaagaa aaaatcggtg
     1741 caagcacaaa gggaataatc agactgatat taaatacaat aggcaatcca aaaataatag
     1801 gttcgcttat attgaatata gcgggagtta ccgataattt agcaaagtcc ttataatctt
     1861 ttctacgact gaagataaat attgcaatca ataaagcaat cgtatttccc gatccaccca
     1921 ttaccgcaaa ggtatcttta aaggccatat tgataatatg tggaatttgc tctttgtttg
     1981 cataagctgc catattttct tgcatattct gaatcaaaaa cggatctaac aatgaacctg
     2041 atattactgc ttgatggata ccaaatccaa aaaatagatt agcaatagat gtaattaaca
     2101 aaaatcctgg taaaccagtt gtaattttac ttaatggctc tgtaactatc gtggtaatta
     2161 gcttatttat atccatatta aacaattgaa tgattataaa tgacaataaa gcaaacgagg
     2221 taatcgtaat aatgattggg attaatacat taaaagactt taaaacagcg ggaggaatat
     2281 ttccggatag ctgaatctgt agtttcttat tattagaaag tttaataaat aattctgtag
     2341 ccgctaatcc aacaaatata cctacaaaca ttcctgaagc tcctgtatag gatataggta
     2401 tcacacccgt cacttcaaca gctttatccg cattttcagg agtaaacatc atctgcatag
     2461 gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc tgcaatatga tttgtataat
     2521 ttctgcttgc acacatatga taagatactg ctgctacaac taataatgta gttatgctta
     2581 atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt ggtcaatgta ctagtatcga
     2641 caatttcccc catgaatcct gttggttcta agatcactga attaataagt gtcacaaatc
     2701 ctgctaacat cataaatggc ataaatgtag caaacgcatc tcgcaaagtt cttaaatgga
     2761 tctgattccc caacttagtc gcaactatac caaatttttc aaatacaatg tccttcctgc
     2821 tacttttgcc catatttttt ctcctttatg cttattgtaa tcgattactt tttccaccca
     2881 aataatacat cacttatcaa aaaaagtaaa tacttttaat taaatatata tatctttttt
     2941 agtataaaaa aataagctct gaaaaaataa ttttcccgag cttatttttc aacaaaaaca
     3001 gcttcaaaaa caaagtcttg atataaatgt ctcgataccg aatactcgaa aggtttacca
     3061 gacgatagag caacaacttt gtctatctca atcataaatt cacctggatc tatttttaat
     3121 atttttgcgt caaattcatt cgcagtatct cctttgatac gtatgtgcga agtctttatt
     3181 tccagaccca gttcatttgt gatataagca tatacagatt tctttaaatg ttttggttcc
     3241 aagcctggta tgactgctaa tggcatgaat gtttgctcaa tcgagtatgg ttgacaatca
     3301 ataaaacggc ttcgtacaat attatataca tagtctttta cactgatact taatttatca
     3361 gctacttttt tcgatggaag ttcaattgag aaagtatgga tttctgaatc tacgtgttct
     3421 tcaccaacca cgctaaacaa accttcgttt ggaccaagaa ctcgtgagtt atcaattggt
     3481 gttctgacga cttcagtccc atacccactt ctacttcgca aaactccttc ggaaacaagt
     3541 aagtctacgc ctttctttac tgttaacttg cttgtatgat aaataactgc caattcatct
     3601 cctgtaggaa gtttctcacc aattttatac tctccattta caattttttt ctttatatct
     3661 aagtaaattt ccatatactt cgcaattttt ttcaatggtg accctccaat caaaaacata
     3721 tcttcatcta tactttaaca taaataaacc gataaaatcc acatcaaaat tatacacaca
     3781 ataaaaaaga tatatatatt taaacaaaac tatttacttt aagttgtaag cggtgttatc
     3841 attaagtgga aacaggaggt aaaaaaatta tgacaactat gaaaaagcta acttttccag
     3901 atgatttttg gtggggttcc gcttggtcag ctgagcaagc agaaggaaaa ggagaaacag
     3961 gaaaagcaga gactatctgg gatcgctggt ttaaagaaca accttaccgg ttttatcaac
     4021 gaatcgattc ctctattaca accgatcaca tccatcaata taaagaagat atagaactaa
     4081 tgaaaaaaac tgggcataac tcctttcgcg tttctatttc ttgggcacga atgtttccaa
     4141 atggcggtac cggaaaagta aatcccaaag ccatttcttt ctatcgaaaa ttattttccg
     4201 aaatgaataa taacggaatc aaagtatttg ccaacttata tcattttgac atgcctgcta
     4261 ctcttcaaga taaaggagga tgggaatcac gtgaagtagt tgatgcttat gttcattacg
     4321 ctgaaacttg ttttaaagaa tttggtgact tagtttgtca ttggtttacc ttcaatgaac
     4381 cacttgggcc tattttaggt acttatcttg aagattttca ttatccaaac ctcattgact
     4441 ttaaacgcgg agcgcaagca gcattcttca ctatacttgc acacgcaaaa gcaatacaag
     4501 tatttaaaga actagctctt cctagtaaaa taggcgttat tctaaatttg agtcctactt
     4561 ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga aatcgctgat ttattttata
     4621 cgcggagctt tttagatcct atggttaaag ggaatttttc taaaaagctt atcctgttat
     4681 tacaagaata taatcagctt cctgaagatt atacgaaaga agatcttgta tgtatctacg
     4741 aaaataccgc tcaaattctt ggactaaact attacgagcc acgtcgcgtc aaatctcgtt
     4801 cttctatggt ggatacatct agtccatttt taccagagtg gtttttcgaa ccacatatca
     4861 tgcctggacg acgaatgaac gaataccggg gatgggaaat ttacgaaaaa ggaatttatg
     4921 atttgtgcat ggatatcaag aaccattatg gaaacatcga agcatttatc tcggagaacg
     4981 gaatgggcgt tgcggatgaa gaacgtttca tggatgatac agggcaaata attgatgatt
     5041 atcggattga gtatatcaaa gatcatcttg cttacttatg gaaggcaata aatgaaggat
     5101 gtaacattaa ggggtatcat ctttggacat ttattgattg ctggtcatgg atcaacgcct
     5161 ataaaaaccg atatgggctg gtttcactcg accttactac tcaaaaaaga acaattaaaa
     5221 aaagtggcga attttacaag caattaagtg atacaaatgg atttagatac gatgtagact
     5281 tgttagttga ataatattct aaaattccac ttgaagtgaa cacattagaa aacaagaata
     5341 atgagaaata ccaataacaa atactttttc ttcatacttc atttatactt gtcagtataa
     5401 gagactgtga caaatgtagt gatcctcaga agttaggtct ggaaatctaa cttgagaaga
     5461 tttttttaaa ctacgtccag tcttttttat ttgtttaaaa ttgaagttga caagtcttca
     5521 ttaaaaattt agaatattca caagtttgac gacaattatg aaaatttatg tcgtgctaaa
     5581 ggagttttgt tattggatga aaagttagat ataaaaactg cgatcaaaga cacgttacct
     5641 accgtttttg gttatatcgg tattggactt gcatttggta tcgttgggaa agctgccggg
     5701 tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct atgctggttc tgcccaattt
     5761 atcacggtca gcatgcttgc tagccatagt ccactgctct ccatcgttct ctcgaccttt
     5821 ctagtcaatt cccgaatgat tctgatgagt atgacgattg ctccttattt caagaaaaat
     5881 cggctgcttc aaaatctgct gattggcaca ttgctgacag atgaaagttt cgcactggga
     5941 atgaataaat tgaactatac tggacaaaag ttaaactttc gttggatgaa tacagctaat
     6001 tggatttctt atttgacgtg ggtgggttct tctcttgttg gcgcactttt agggaatttt
     6061 attactgatc cgaaaaagtt tggtttggat ttcgccatcg tcgctatgtt tatcgggtta
     6121 ctttatcttc aagtcatttc tgatagaaac acctctaaac gattacagtt gatcctgatt
     6181 ggtctcactt tgattctggt atatgtcgga ctgattttta tcccaagtaa cttagtgatt
     6241 ttagttgtaa cactgattgg gtgcggattg ggggtgtgga tcaaacatgc cttcttttga
     6301 gtttacattg ctgactattt taggttgtac tttagcaact tggctttcta ggatcctacc
     6361 atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg cttgagtatt taagttttgt
     6421 cccgatcgtc attatgtctg ctttatggtt cagcagtcta tttacccaaa atatcggaca
     6481 tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt cctacacttt tagctgctat
     6541 tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt ctttcccttt cgttgattcg
     6601 cttattataa ataaaaaaac aagagcttat ggcaaattac tgtcatgcgc tcttgttttt
     6661 atcaaagtca aataagaaaa aaatcggcgt attcttgctt atccgttgaa cggaccacta
     6721 acaacgactt ttcctaatat atgtcctgtt tctacgtctt tggttgcttg tttcaagttc
     6781 ttcgcattca tcccatctga atagaccttg ctcaatgtac tgcggataat cccttcattt
     6841 gctaaagcag caatgtgcgc taaagcttct ccttgcgttt cgatttcata gttatagtct
     6901 gttttagcga acatatactc ccagtcgaag ctgatagatt ggtttttcca aacacctata
     6961 tctaacggct ctttgatccc tacgatcgtt cccacatgtc caaatggtcg gacaaggtgt
     7021 ttgatttgat caaagtagcg ggtaatatcg aataatacag cgacatactc tacttgatcg
     7081 attcctaatt cacgaaaact tttacttaag tcgtgatgat aatcgattgg atagtctaca
     7141 cctgtttttt tcagccattc aaaattcttc ggacttgcag tcgcgtagac ttctaggcca
     7201 gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc cagctccgtt gatcactaag
     7261 atttttttcc cgcggttggc attttcttct ggagtcagac caaatttttc aaataacaat
     7321 tcataagcgg tcaatgaggt caacggcaag gctgcggctt cttcatctga aagatttttt
     7381 ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt cttgattgct tcctgctctt
     7441 gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa atttttgcac ttcatcacct
     7501 acagcaacaa ctttcccgac tccatcaaac cctaagatac gtaaagcatt tcttatcccg
     7561 ttatcttggc gaagtttcgt atcgacagga ttaacagata ctgcgttgac ttttaccaga
     7621 agatcccgtg aactgggtac aggtatctgt tctttttcat caataaagct ttttggatca
     7681 tcgatcgaca acccttcata aaaaccgact accttcatta attccttttc tgacatacga
     7741 attcctcctt agcttcatca agttggagca tccatacagt tttcatttca atatatatag
     7801 tatgctattt gttccacttt tattcatcat aattattctc ttacttaaag taaactattt
     7861 gcactttttt ggttaaaaaa gaggttgcga caagactttt gtcacaacct cttttaaacg
     7921 gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg acaaaatatg aggagctatt
     7981 tttgtttggc tgttcttatt acttgatgca ggacagcttt ttcacgacct ctaattcacg
     8041 gtgttcaagc aagaaatcat tgttcactga aaagcgttgg atgttctttt tcaaaggtgt
     8101 ctaagatctc tcgtacttca tcagtagatt tcgtattcat caactgaact cgcaagtcat
     8161 ttgctcctgg aaatcctttg atatagattt taaagaaacg atgtagtcct gtgatggaac
     8221 gcgggatgat ttctgaataa tgatcttgca gatctagttg cagacgaagt agtccgatca
     8281 gttctttttc tgtatgttcc ctcggttctt tttcaaatgc ataaggattc ttgaaaatcc
     8341 cccggccaat catgatgcca tctacgccat atttttcggc caattcttct cctgtctggc
     8401 gatctgggat atctccgttg atcgtgatca gtgtttgcgg agcgatttgg tcacgtaaag
     8461 caatgatttt agggatgact tcccaatgtg catctacttt actcatctct ttacgcgtac
     8521 gcaaatggac agaaagattg gctatatctt gatgaagtag gtgactgatc cattcttcca
     8581 tctcagacat ttccgtatag ccgatacgcg tcttgacgct gacaggcaaa ccgccagctt
     8641 tagccgcttc aatcaattgt gccgctactt ctgggcgaag gatcaagcca ctgccttttc
     8701 cacgttctgc tacattaggg actgggcagc ccatgttgat atcgattcct tggaagccca
     8761 tttctgccat agcaatgctc atttctctaa aaaattctgg cttgtctccc caaatatggg
     8821 caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc tcgtacactg tcttttccat
     8881 ctggatgaca gaaactgtct gagtttgtaa actcagtaaa gaagacatcc ggtgctgctg
     8941 catgctttac gacatggcga aatacaacat ctgttacatc ttccattggt gctaaaacaa
     9001 aaaaaggctt tggcagctct ttccaaaaat tagtagtcat ttatttgtta ctctccattc
     9061 tatcaagcag tgaagtcgca atctttctca aaaacattgt cattatacta ggaaacaaaa
     9121 aaagaagcaa agtgaacagc ttaaaagatg tttctgattg agcaaaaaaa gactgtgaca
     9181 aaaatcttat cacaattcct tttccggaat aaatggtgtt caaaagcttg catctgcggt
     9241 aataagtcca accaaacgaa aaataggagg agctattttt gtttggctgt tcttattact
     9301 tgatgcagaa cagctttttc acaacctctc cactaaacgg tgttcaatca gctgaccctc
     9361 ggtattagct caacaaacga caaaatatga ggaactattt tcccgtttgt ttctctaata
     9421 ctcaggtcaa aacgctgatt tcacaacctc ttttctaact tctcaatcct cttcctttat
     9481 tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa tagcacaagt acgatcatcg
     9541 atgttgctac tggcacatcg attgttccaa ggaatccgta acggaaaccg gagatcatat
     9601 aaacgatcgg attgactttc gaaacagctt gccaaaatgg cggcaacata gagatggcat
     9661 agaaaacgcc acctagataa gttaacggct gcaagacaaa tgtaggcacg atcgatacat
     9721 cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc taacgaaaac aagattgccg
     9781 tcatcaataa tgtaatgatg accatgaacc atgaatacac atgaagtggt acaaagaaca
     9841 aggaaatgat cgttacgaga gagccgacga gaatactgcg acccactccg ccaatcacaa
     9901 agccccaaat gatcacatgc gtaggaacag gcgctaccaa gatttcttcg atgtttttct
     9961 ggaatttttg agagaagaag gacgaagaaa cgttggcata cgaactagtg atagcagaca
    10021 tcataatcaa accaggcacg atgaattcca tatatgaaaa accacccatg tcaccgatcc
    10081 gaccaccgat catttttccg aaaataacaa agtacaagga tgtcgtgatc acaggcggaa
    10141 ccagtgtttg gacccagata cgcaaatagc ggttcgtttc cttgatagct aaacttttta
    10201 atgcagtaaa ataaagatta aacatttttc tcctccgtat gattattttc ttctgtgatt
    10261 ttcaaaaaca gttcttctaa acgattcgac ttattcctca tagaaagaac tttgattcct
    10321 tgtcttgtca gctgatcgaa gattccgttc accccttgat ttcgttcaac ttcgacagcg
    10381 attgtctgtt catcttcaaa tgtacaagcg tatccttcaa tcacaggttt ggtctcatat
    10441 ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg atagaagctc tttcatacta
    10501 gtattctcga tcagttcacc tgattggatg atcccgatgt tgcgacaaag catttcagct
    10561 tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat tttcgttcaa ttctcggaga
    10621 aactcccaca tctcgcgtct caattcaata tcgactcctg ctgttggttc atccaagatc
    10681 agcaaacgtg gttcatgcat caatgcccta gcaatcatca gtcttcgctt cattcccccg
    10741 gaaagcatac gtgcacggac atttctcttt tcccaaagat tggactgctt caaatatttt
    10801 tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt agcctgcttg attcacgacg
    10861 atttgctgga ccgtttcaaa gggattgaaa ttgaactctt gcgggactaa tccaatctgt
    10921 tgctttgctc ggactagatc cgtatccaga tcataaccga agacattcac ttttccagat
    10981 gttttattga ctaaagaagt aatgattccg atcgttgtag acttcccagc tccatttggt
    11041 cctaatagcg catagaaatc tccttctttc acggaaagat cgattcctcg caatgcttcg
    11101 accccacttc cataaacctt tttcaaatta tttatttcaa gcgcatttgc catttatttc
    11161 ctatctcctt atagacatga ttctttattg tatcatggaa tacagcagat gaaaaaacaa
    11221 cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa atctcctaat gaaagacatc
    11281 aatcggacga agtcagacaa ccttttcaga aaaaacaaat cgaagataaa ggattactat
    11341 tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct
    11401 aaacacgtat aaacggcgga agcaaaagcc aagatttttg atccaataaa tactttcgat
    11461 cacttcgcct atcccaatac atgccactgc cgcttttcat caaataacgc ggcttccgtt
    11521 tttgcggttt ctactaaagt ctctgggtaa tccaaaactt tcagcaaggc aatcgcattt
    11581 cgggtccttg aaggtccttc ttttaatttg aaatcaaaac tgattccttt tccttctgtt
    11641 acttgttctt caaagtggac attctcacaa taattcttta aaatctctgt caattcgata
    11701 tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa gccaccgaac gactgaagcg
    11761 gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa tctcatctac gaagcagtag
    11821 cagcgttctt tggaagccac ttggtccaac aaacgtttga tcgatttgat ttctgaaaca
    11881 aaataactgt cgccttcaaa taaatcatct tcgattgcca tggaagtcaa cacatgtcca
    11941 ggttgcaggg taaactgttc tgcaatcgct gtttggatcg tctgtgctag aatacagctg
    12001 atggctatac ttttgacata cgtagatttt cctgatgcat tggaacccgt gactaatgta
    12061 ttcttctgcc agttgaccgg attcactacc gcttctttta acaatggatg atagatatct
    12121 ttcgctgtca caccgccctc agtaaactct ggtcggcaag tgatgggcat ataagtacgg
    12181 taattcaata ttgcagcggc tacttctaat tctcccagca attcccacag cttgatggct
    12241 tcagttgaat gcttttctaa tttagtgaga acgaaatgat aggagataaa tggcagcatg
    12301 aacatgatgt tgacatattc aaaaatcaac tctgcttcac tcccactctt catacgaaat
    12361 gaaaaaccta atttagggat gtctcttaat ggtttagttt gctgtttcag ctcttcttga
    12421 agtggtgtcg gcagttttgc tagctgattg gcggctgaaa tcgttgaaac caaataactc
    12481 atgctattca gctctgtttc tagtgtttgt ttcttcacta gataatagac cgtattgaat
    12541 actaacccac cgattgccaa atagattcct agtacttgtc ctaaaagaag caaacccaaa
    12601 cccaccagcg gaagcaagcc aagaaaaata aataaaccta cgcgtccaat ctttttactt
    12661 tttccatccg ctagatattg ttttgcaaaa ttatgatctt tttttcctaa acgagcgaat
    12721 tgatattgga ttttttctct cagttgagga ttttcctgat aaaaagcaat cagtttttct
    12781 aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt gatacaacgc ttctgaacct
    12841 acactagaat aggtactgtt gattccttcg aatacttcga acatatccag gtcataccaa
    12901 gtaagatcat cgatttcgct atcccatgaa cggaacttct tctccgtgag ccatgcttcc
    12961 ttcaagcttt cttctttatc aaatctcggt tgataaggaa ttttttccca tttgctacga
    13021 actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag caactaataa aataacagct
    13081 gcaataataa ttaaaacgat aaattctctt tgacccatat ccctctccgt tcctttattt
    13141 gttagcttta gtatagcact gttgcgctat ttgaaaagag ggtaaaaaaa cacctttcaa
    13201 aataaaaggt gtttttttct tgtatattac atatttatct ctcatttatc aaaattgaca
    13261 aataatcata gggaggaatt gttgttaatt cttgtttatt ctccagtgca ttgacaactg
    13321 tcccaaggga aaggatagcc tcaggtacta ttttttcttg ttctgttgga ttagctaagc
    13381 gaagaacaaa tccttgttca tgaaaataag aaggataaat cgatgataca attaagtctt
    13441 caggtaattc aatcaatgaa aatttacgag gaacatcaag ttcattttgt aatggccaaa
    13501 tcttgttatc taacctgtga ataaatacat tcagtttctg cttttgataa gaaatagatt
    13561 gttctaaccg tttttctgcc tgttttgcca ctttgaactc attaaatctt ccttcttcta
    13621 cattaaatcc aaaagtgact ttatactcac ctatctcttg agctagcggt gtttccatca
    13681 tgacatgccc ttcatttgtc gtatctcctg aagctcgccc aggtcgccaa gcaagattag
    13741 gttttccaag ttcccctgtt gttgaaaata aggttatgat taattgattt tctatacgtt
    13801 catactcttt taaaccatct gcataaaacg taaaataatg atcattttct gccacagaga
    13861 cactcttatc gaatatttct aaattaacag gtttctctac catagtttct gaccaattcg
    13921 gaggaatgac atcaggggta ttttcaataa atccgttttg aatttgtgca attgattttt
    13981 cagatttatc taaaacatta attgctaagc gcatacgatg cgagtaaaca gtattatgaa
    14041 ttttgagtgt gccttcaacg atttgacttt ctcccagact gaacgatatt tcataagata
    14101 tttcttccat ttcgggtttt tcagataaac gatcttttaa atctttagga agttgagctt
    14161 ttccgtaaac aactaatgtt tcttgtaaag aatctttata tactttagct gtttctaatt
    14221 ttagagatag ttctgtatct ccttctaatg gtgaataatc atacgtatcg ccatcatttg
    14281 ctgaatcgat caaatgaacg aaatcaagat attgtctatc tccaactatc aaattcaatt
    14341 gcccatcttt aaaagaaagt ttatatttat tgttacttat ctcagttttg tctatctcga
    14401 ttgctgtttc taatttttca ttatttttct caaattcaac aacagtgtat cctagtgctg
    14461 gcacatttac atttattttt atgtcaagtt cataataagc tggttcatca aaatattcga
    14521 aacctgtagc aatcatccga taagcatttt tccgttctgg aaaataaact tctttttcaa
    14581 tgatattata gggactacct ttgaagtgta ttttctttga atcagctact acatgcacga
    14641 ttttcgatcc actataattc tcagtcgttg tgttgaaaac aaggacttca ttcgatttta
    14701 gctttaatgc atcagccatc cgttttacaa taaggttttc gattccgtca gctatctcat
    14761 tagcttcttt gattcgatgg aaaatatctt ccgcaacatt atctgaaaca cagccaccaa
    14821 tactatcatg agcctggttt tgcaaaatct ttttccataa ccgacgaagt aggcctttac
    14881 tgatatggat accatttctt tctgcaatca gcatgagcgg ctcaactcgt tttagaattt
    14941 tttgttccaa tttaaaattt tctattttca tttttgttct tactgagcca atagaacgat
    15001 gaacacgaga gtaaaccggc tgacgaagtt cacctttata ttcaggtaat tcttttttgt
    15061 ttctgataac ttctacataa gaaggataat ctgaaataat attgtggaat ttactgtatt
    15121 tattcaactt ctctacgact tgatcaaaat ttaaaatcat tgacttttga tctacacccg
    15181 aaggtactaa aatatctttg ttttttcctt gcatatgaag catttcaact gcttcatcca
    15241 acttttcttt tgcaaattca ggaatatctt taattgcgtc taaggtcatc attccagtca
    15301 tataaccaaa tggaaaattc atggcataaa caaatttatc tcccaatcct ttccagataa
    15361 aataaggaga tggaaccatc gtttcaaagt ttaatcctcg ccaacacatg aaattatcga
    15421 tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa cccaaacgtg tcaggcaaat
    15481 accctaccat cattggatct ccatatttaa ggatcgtatc actgattcca atcattaagt
    15541 ttcttaaaat tgattcagca tccactaata aggcatctgt ttgtgcatac cacggtccca
    15601 cgaatagttg tcctgttttc atcagtcgtt ggataacctt aagcttttgc ggattgatct
    15661 ccaaatactc atcgacgatt gaagtttgtc catctaaaca aaagtttact ttagggttcc
    15721 tttccaattc atctagaact tcagtaaaga cctgatcaga caatacgatg gcatcttcat
    15781 ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat tatatgtgca tttaccatat
    15841 cagattctcc tactttcctt cttctactga atttacttgt tgttcttttt tatacttgat
    15901 ggattttagt gcattgacca ttaacgctgt aaccactccc ccaataacaa ttgatactaa
    15961 atataaaggt atattcgtaa caaaaggaat tgccataaag gttgaaagca ttgtttcttg
    16021 agtaacacca aaaaatccat ttagaccaca tgctactgca gtgcccacca cgatgcttgg
    16081 tattactcgt aaaggatctt tcacggcaaa cgggatcgtc ccttcagtaa tccccacaag
    16141 gcccataatc atagctgtct ttgcattatc tcgttccgat tcatcataaa gttttttgtt
    16201 taaaattgtt gctaaaaaca ttccaaatgg cggaatagca attcctaata acatggctgt
    16261 actaggtcca aagcttccgc ttgaaaaaga agcaacccca aaagcatatg cgattttatt
    16321 gataggtcct cccatatcaa acgccatcat tcctcctaaa atagccgaca tcaaagcagc
    16381 atttgctcct gacatgcctt ctagccaatt agtcagtcct gttgtcattg cttgaatagg
    16441 cgtagctagt acataccata atatgattcc aacaactaat gtagtaacaa aaggaatcag
    16501 cataattgat tttagactaa taacttgcat cggtactttt acttttttca atgcattagc
    16561 taaatagcca caagctaatc cagcaaccat tcctccaata aatcccatac caagattttg
    16621 acacatcatc ccagtgataa aagctggtgc tagaccggga cggtcagcaa ttgaatatgc
    16681 gatataggct gaaatcatcg gtacgataaa ctgtaatccc gtctgaccaa tcattcgcag
    16741 gactccccag aatgtatccg tctggtccca aacacctgtg ccaccaaaaa tgactgcaat
    16801 agaagttagt aaccctgctg ctacgacaag cgggatcata taggaaaccc cactttggaa
    16861 atgatttaga atttctcttc cttgtttctt gaattccatt agtaactccc ccacgatata
    16921 ttttttattt gttaattaat gcaattgctt catcaatcgt ctcttcacca tcttttacta
    16981 cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa gcgacctcgt tctgaaacat
    17041 ttgtatctac tgcaaatatg acaacatctg cctcggaaac atccgaaatt gaaagtttgt
    17101 tttctatccc catagccccc tgtgtttcta ctttaatatc taaccctttc ttcttcgcaa
    17161 acttttctaa attagctttt gccatataag tatgggcaat ccctgcgata catgctgtta
    17221 ctgctactat ttttgtcata tgaaattcct ccttttaaaa tgatagagct tttaccaatt
    17281 cttttttatt tctcgaatct tttattcttt taacaaactc gtcatccatc aatcttccag
    17341 ataattctgc taaaatacgt aaatgattag tacctttatc agtgtctttt attgctaaca
    17401 aaaagacgag atcaactggt tgatcatcta atgcgcccca ttcaacagac cgctttaact
    17461 tggctacagc cactgttgat tcaattactg ctaggctttt gccatgagga atggcgatat
    17521 ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa tacgtctttt tgaaattgtt
    17581 tgtggtcaga cacaaaccca ttagtctcta acaatttcga taatttttcg actacacttt
    17641 ttttatcaga tgcatttaaa tctatcacta tattatttat attgattttt tcactgatca
    17701 tttataagcc ttctttcaat atataaacta atttttcaaa cgacgaactc gtttttaaat
    17761 ttgctaataa ctcatgattc gaagcgattt cattgaatgt ttcataaatt ttattcaatt
    17821 caggttttac tttttcatta agtgcaaaaa agaaaacaga atgaacaagt gtcccatccc
    17881 aatcaattcc ttctggatta acgtagacac agattactgg cttttttata aaatctactt
    17941 cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga tagtttttct cttttgattg
    18001 ctccctctag tatcccttct ttcgcgtaat tttcttttac caattttttg gtaattatat
    18061 ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa aaagcaaaat cgtttatcta
    18121 ataaatttag aaaagcatct tctgtttgtt catttaactg tagatactgt tttacttttc
    18181 tgacatctgc ccctaacatc aaaggactaa caacaataac aggaaaattt gtattttcaa
    18241 tcggaatcgt gcttataaca agttcatcag tgacttttct atcttgtagt tcattgatgc
    18301 ttagtacatc ttctatctca accatatctg caaaaccgtt atttattctt tgctctaata
    18361 attttgatgt tccatatccg ctactacaaa ctaatatcac tttcgttttg tcaggtttgt
    18421 aacgatccaa aagtgattga atatggagtg ttacgtaagc gatttcatct tcattaaaat
    18481 gtatacagaa tctctcttcg atttcctcta ataaatctac acttatcata aatgaccttt
    18541 taaaagaata ttt
//
LOCUS       DASJSZ010000055        18504 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.055, whole genome shotgun sequence.
ACCESSION   DASJSZ010000055 DASJSZ010000000
VERSION     DASJSZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.055"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..746
                     /locus_tag="U4B03_001800"
     CDS             99..746
                     /locus_tag="U4B03_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN2068648.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="U4B03_001801"
     CDS             1139..3784
                     /locus_tag="U4B03_001801"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEN2068649.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="U4B03_001802"
     CDS             4094..5407
                     /locus_tag="U4B03_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEN2068650.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="U4B03_001803"
     CDS             5397..6050
                     /locus_tag="U4B03_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2068651.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="U4B03_001804"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="U4B03_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEN2068652.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="U4B03_001805"
     CDS             6947..7147
                     /locus_tag="U4B03_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2068653.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="U4B03_001806"
     CDS             complement(7401..9080)
                     /locus_tag="U4B03_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN2068654.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="U4B03_001807"
     CDS             complement(9082..9294)
                     /locus_tag="U4B03_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEN2068655.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="U4B03_001808"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="U4B03_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEN2068656.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="U4B03_001809"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="U4B03_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEN2068657.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="U4B03_001810"
     CDS             13269..13466
                     /locus_tag="U4B03_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068658.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="U4B03_001811"
     CDS             13619..14104
                     /locus_tag="U4B03_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEN2068659.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="U4B03_001812"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="U4B03_001812"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEN2068660.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="U4B03_001813"
     CDS             14981..15508
                     /locus_tag="U4B03_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068661.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="U4B03_001814"
     CDS             15599..16336
                     /locus_tag="U4B03_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN2068662.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="U4B03_001815"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="U4B03_001815"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEN2068663.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="U4B03_001816"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="U4B03_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEN2068664.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5983 a         3200 c         4022 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag ttgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DASJSZ010000056        17654 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.056, whole genome shotgun sequence.
ACCESSION   DASJSZ010000056 DASJSZ010000000
VERSION     DASJSZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.056"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            93..323
                     /locus_tag="U4B03_001817"
     CDS             93..323
                     /locus_tag="U4B03_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEN2068665.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            420..1721
                     /gene="murA"
                     /locus_tag="U4B03_001818"
     CDS             420..1721
                     /gene="murA"
                     /locus_tag="U4B03_001818"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN2068666.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1734..1910
                     /locus_tag="U4B03_001819"
     CDS             1734..1910
                     /locus_tag="U4B03_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN2068667.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2045..2341
                     /gene="yidD"
                     /locus_tag="U4B03_001820"
     CDS             2045..2341
                     /gene="yidD"
                     /locus_tag="U4B03_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEN2068668.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2526..2993
                     /locus_tag="U4B03_001821"
     CDS             2526..2993
                     /locus_tag="U4B03_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2068669.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3078..3962
                     /locus_tag="U4B03_001822"
     CDS             3078..3962
                     /locus_tag="U4B03_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2068670.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4076..4312
                     /locus_tag="U4B03_001823"
     CDS             4076..4312
                     /locus_tag="U4B03_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEN2068671.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4400..4480
                     /locus_tag="U4B03_001824"
     tRNA            4400..4480
                     /locus_tag="U4B03_001824"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4434..4436,aa:Tyr,seq:gta)
     gene            4487..4558
                     /locus_tag="U4B03_001825"
     tRNA            4487..4558
                     /locus_tag="U4B03_001825"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4519..4521,aa:Gln,seq:ttg)
     gene            4683..4853
                     /locus_tag="U4B03_001826"
     CDS             4683..4853
                     /locus_tag="U4B03_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068672.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5073..5780
                     /locus_tag="U4B03_001827"
     CDS             5073..5780
                     /locus_tag="U4B03_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2068673.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5839..6483)
                     /locus_tag="U4B03_001828"
     CDS             complement(5839..6483)
                     /locus_tag="U4B03_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEN2068674.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6609..6896
                     /locus_tag="U4B03_001829"
     CDS             6609..6896
                     /locus_tag="U4B03_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068675.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7004..7324
                     /locus_tag="U4B03_001830"
     CDS             7004..7324
                     /locus_tag="U4B03_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2068676.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7405..8718
                     /locus_tag="U4B03_001831"
     CDS             7405..8718
                     /locus_tag="U4B03_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2068677.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8715..9434
                     /locus_tag="U4B03_001832"
     CDS             8715..9434
                     /locus_tag="U4B03_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEN2068678.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9562..10113
                     /gene="raiA"
                     /locus_tag="U4B03_001833"
     CDS             9562..10113
                     /gene="raiA"
                     /locus_tag="U4B03_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEN2068679.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10335..12869
                     /gene="secA"
                     /locus_tag="U4B03_001834"
     CDS             10335..12869
                     /gene="secA"
                     /locus_tag="U4B03_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEN2068680.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12995..14096
                     /gene="prfB"
                     /locus_tag="U4B03_001835"
     CDS             join(12995..13066,13068..14096)
                     /gene="prfB"
                     /locus_tag="U4B03_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEN2068681.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14201..14887
                     /gene="ftsE"
                     /locus_tag="U4B03_001836"
     CDS             14201..14887
                     /gene="ftsE"
                     /locus_tag="U4B03_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEN2068682.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14871..15764
                     /locus_tag="U4B03_001837"
     CDS             14871..15764
                     /locus_tag="U4B03_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068683.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15916..17364
                     /gene="cls"
                     /locus_tag="U4B03_001838"
     CDS             15916..17364
                     /gene="cls"
                     /locus_tag="U4B03_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN2068684.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         5908 a         2972 c         3828 g         4946 t
ORIGIN      
        1 atttttaagt atacttgttt ttccttttat acattttatg gtattatcat tttattagtt
       61 attataaaac aatcgttaga aaggacgtca ttatgcaagt atacggaatt gatgcggtgc
      121 ttcgaatcgt gagtcatttg gcgtttatct acttagcctt ctggtcttta cgttcgttac
      181 gcattgaaac ttttttcaga ccaatgcata atacgcaaat tagaatggca atcgtacttt
      241 tttcaatttt tttagggtac actgcaagta attttttctt agaagttatc gctttatgcc
      301 gaaatctttt cgttagcctg taaattttaa aaaaaggtac tattttaaaa gcaaggatgt
      361 tataatagaa aagattttga gtaagtgtat gctcattgga attttttgga gggaacaaca
      421 tggaagagat catcgtaaga ggtggcaatc aattaaatgg aacagtaaga attgaaggag
      481 ctaaaaatgc tgttttgcct attttagctg ctagtttgtt agcagaagaa ggtattacga
      541 cattagacaa cgttccgatt ctttctgatg tcttcactat gaatcaagtg attcgtcact
      601 taaacgtaga tgtagatttt gatgaacaaa agaatcaagt aacaatcgac gcttcaagac
      661 aattagaaat cgaagcgcct tatgagtatg ttagtcaaat gcgtgcttca atcgttgtga
      721 tgggtccgct acttgccaga aatggacatg caaaagtagc tatgcctggt ggatgtgcga
      781 ttggtaaacg accaatcgat ttgcatttaa aaggatttca agctttagga gcaaaaatta
      841 ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt gatcggtaac acgatttatt
      901 tagatttccc tagtgtaggt gctactcaaa atatcatgat ggctgccgta aaagcaaaag
      961 gaacaaccat cattgaaaac gtagctcgcg aaccagaaat cgttgattta gcaaacattt
     1021 tgaataaaat gggcgctcaa gtctatggag ctggtactga aacaatgcgt attgaaggcg
     1081 tggatcattt acatgcagtc aaccactcaa tcgtacaaga tcgtatcgaa gcaggtacct
     1141 ttatggttgc tgctgcaatg acacaaggca atgtgctgat tgcagatgct atctctgaac
     1201 ataaccgccc attgatttct aaattgatcg aaatgggtgc agaaatcatt gaagaagaag
     1261 gcggagtccg tgtcatcgga ccaaaacata tcttacctac agatgtaaaa acgatgcctc
     1321 atcctggttt ccctacagat atgcaagctc aaatgacagc tatccaatta gttgcagaag
     1381 gtacaagtgt agtgactgaa acagtgtttg aaaatcgttt ccaacattta gaagagatgc
     1441 gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc aatcatggat ggaaaccatg
     1501 aattgcaagg tgcagaagta tatgcaacag acttgcgggc agcagcagca ttagttttag
     1561 ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt gaattatcta gaccgtggat
     1621 attataattt ccatatcaaa ctacagcaat taggtgcaga cgttgaacgt gtagacatgg
     1681 atcaaacttc tgctgaaaaa acagcacaaa cgattgctta aggaagtggc tgaatgagta
     1741 accaacgata tatcttatta actttgataa agattttaat ggtgatctta ttgcttatcc
     1801 ttctttttgt ggccggaacg atgatcggat atggtgtcat cggtggcggc aatccattta
     1861 aagtatttca gccaagtttg tggattcata ttcgagactt ttttcattga taaataagaa
     1921 tcacagtggt tctagtcgat atgaagtgag ccatgaagca gaacatgata aaagaaaaca
     1981 gaagggaacg ttgacgttag tcgcgatccc tttttttagt aaaagaaaag aaggaatcta
     2041 attgatgaag aagatattga tttatttagt tagaggatat cagcgcttta tttcgcctgc
     2101 atttccacct agctgccgtt attacccaac ctgttccaat tatatgataa aagcaattca
     2161 aacccatggc gcgctaaaag ggacggtaat ggggactgca cggatcttac gctgtcatcc
     2221 ttttgtcaaa ggcggtatcg attatgtacc attgacattc cgattaacgc gaaataagaa
     2281 agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat acgggtagca ctgagcgata
     2341 aagaaacaat ttggaaaata gcttcttata ttttgcaaaa ttgtgagtta ttgccgaagt
     2401 gttggatttt gaacaccgtc aaaaaaaagg ttgtgaaaaa tacggctagc actgagcgat
     2461 aaagaaaaag aggaaattta ttgtcaaagt aacaactccg caactactta tatgggggac
     2521 aaaaaatgat cgaagaacta aacaagaaag atttaccatg gtccttgtta ttagatgcag
     2581 atcctgataa agaaaaagtt caagtatatg tttccagagg gagtggctgg atatggaaag
     2641 aaaaagaaaa aacaataggt gtactgatct atgtggcaag ggaaaaagaa tacgaaatcg
     2701 tcaatgtagc agtaacccca acgtatcaag gaaaggggat tggcggaaaa ttacttgaga
     2761 cagcttttca gaagctttca caactcgctt ccagtcaaac tcagatcatc atacgaaccg
     2821 gcagtactag cagtgcggcg ttacatctct atcaaaaaat gggctatgtg gagattggtc
     2881 gagtcaaaga ctattttatc catcattact ctgagcccat ttttgaaaat ggagatcaat
     2941 taagggatca agtgatttta gcaattcact tacctaaaaa tagctttaac taataaaatt
     3001 tcaattcaag aaaatgcttt caaatgctaa atagatacat tacaatagct ttagcaaaca
     3061 aaaaggagaa tatttttatg ggaatattag tttttgattt tggtggatcg gctgtcaaat
     3121 atggttgttg ggatggcaaa gagattaaag gtaagggaca atttgcaacg cctgaatctt
     3181 gggaagaaat gaaagcgcaa ttatttcaag tttataaaaa aatactcgtt tcttttgaag
     3241 tcgaaggaat tggaatcagc tctcctggag tagtgaataa tgagaaacag gtgattgagg
     3301 gaatcagtgc gattccttat attcatggat ttaatatttt ccgagatttg gaaactctgt
     3361 ttcaacttcc agtaacgatt gaaaatgacg ccaactgtgc cggaatggct gaattttatg
     3421 aaggtgctgc ccgcgattat caagatgttg cttttgttgt ggcaggaact gggattggcg
     3481 gggcgttgtt cacgaaagga caaatcaata aaggggcaca tctttacggt ggagaatttg
     3541 ggttgatgtt cttaaatggc gataagacat tcagtaaact tggtacagcg gtacagatgg
     3601 cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc ttataccgga aaagatgtct
     3661 ttgaattagc agaaactgga gatgcaatag caaaagaaga agttgagagt ttttatacgt
     3721 atcttacaaa gggattattc agtattcaat tttcgtttga tccagaagtg atcgtattag
     3781 gcggcggggt ttctgcaaaa gaagggctca ttgatgagat caaaagccga atgaagaagc
     3841 tgacagaaca attcgatctg catgatttcg aaccgcagat tttattatgt gaatacagaa
     3901 acgatgctaa tcttgtaggt gcagcagcaa actatctcgc tgtacgtcaa ggggagaatt
     3961 aagagcttta gagaaggtgc agcttgcata aaagaagtga atgaaatgaa taggagattt
     4021 ttacctaagg aatgttttat ttgctgcagt atgataaaat atctgagagg tgaaaatgat
     4081 gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa gtgaaaaaag atatcaaagg
     4141 taagaattat gaagtcactc agttatttat agggaaaaaa atgattggtg agatcttagc
     4201 ctatggacca aaagaatatg aaattttctt tggtgaagaa gattttggaa aagaaaaaag
     4261 tcttgaaaat gcgatagaaa cagtgattcg acactggaat ctgcacgaat aaaaaaatat
     4321 caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa ttaggtatta taactaagtc
     4381 aggttgatac aaacctttcg gaggagtagc gaagtggcta aacgcgacgg actgtaaatc
     4441 cgttccttag ggttcagtgg ttcgaatcca ctctcctcca tttcattggg gtatagccaa
     4501 gcggtaaggc aagggacttt gactccctca tgcgttggtt cgaatccagc taccccagta
     4561 taccacaatc gtaagctctc gttagttcga gagctctttt ttttgctttg ctaccttgtt
     4621 ccttgaagat tctgctgatt taagatagag ttaagttaac tacaagaatt ggagggatcc
     4681 acatgaaatg gaaagaaaaa gtagacgaag cagctgaaaa gttatatgat ttggtcaaat
     4741 cagaaaaata caatattgaa gtgaatattc cgaaaaaagg cggaaaagcg gtccgggtaa
     4801 aatcaaaacg cccaacgaat catacaaaaa aatggatggc taaaaaccgc taataataac
     4861 aaaagatcca taagtcggca ttttcatcga ttttagggtc ttttgttatt ttctaggttg
     4921 gacatatcta aaaaattgtt tctgacaata ggagtaggaa gaacgatgtg tgtctaaatc
     4981 ctctgttgat ggatagaaac ctttatcttt ttcagttgtg aatcaggtat ataccagtta
     5041 taatagaaga aacgaaagga cgggtgtacg ttatgtcgaa tcaattacct gtatatatac
     5101 agattcatga ccaattaaaa aatgaaatcg aacaaggtat atggaaagtc ggagaccgtc
     5161 ttccttcaga aagagaatta gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag
     5221 caattcaaaa cttggcagat gaaggaatct tagaaagaaa gatcggttcg ggtacatatg
     5281 ttgcacgaga aaaagtacaa gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg
     5341 aatcacaaaa tcgagtaccg tctagtcgga caatctctta tttcttgact tcgccaagct
     5401 caagtgaaat ggaaaaacta aacttgagta aagatgacac gatcttgcga atggaacgta
     5461 ttcgtttcgc agatgatgtc cctatttgtt ttgaagttgc cagtgtgcca caaaagctga
     5521 tcgatggcta cagcaaagca gagattactg aatcgttgta tcgcacgcta gaagaaaaag
     5581 gcgggaaaaa gatcggtcca gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa
     5641 ttgctgacta tttagaggta aaacgaggag atgcagttct tcgtttgcgc cagatttctt
     5701 attttgaaga cggtacgccg tttgagtatg tgcgtacgca atacgtagga aatcgttttg
     5761 agttttattt ggaaaaataa aaaacaggtg atccctgatt ccgtattaga aataaaagag
     5821 aatcagaatc acctgttttt atatatgatc agttaccagt tgctcgtgat aagaaacagg
     5881 accctcttca aattggactt gtccgttcag cagctcaatg atggattctt taaagtggtc
     5941 agcacttttt tcatctacag cacagatgac gctaacttga tcagtatatt ggatttcttt
     6001 tataaaaaaa tgttggagtt caagataatt ctgtaatttt cctagttgcg gatacgtgat
     6061 catcacagag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg
     6121 agagaccgca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa
     6181 atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga cttccaacat
     6241 cggtatgccc gccgttccac ttggttctcc atcatcgctg cttcgctgga tttcattttt
     6301 ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt tttttatttc
     6361 ttgaatgaaa ctttttgctt cgtcctctga ttctattcgt ctaagagaac agataaagcg
     6421 tgattttttg atctcgatct cactttgtcc attttctttt attgtacgat agcgctcgat
     6481 catcttgtca cctctttttt cattttcttt attcagtata aataaagttc taggttgttt
     6541 caacactgta acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg
     6601 atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca
     6661 atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta
     6721 acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc
     6781 ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg
     6841 acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa
     6901 accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt aacagacgcg
     6961 aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta aaaaaacaat
     7021 catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc
     7081 tgctgaagca aaaggaatcg actctgatat tttcgctgtg tccgcttctg acgcagataa
     7141 caatcttgca aataaagacg tggatgtgtt gttattaggc ccacaagttc gctttatgaa
     7201 agctgatttt gagaaacgtc tggaaccaaa aggcattcct ttagatgtca tcaatatggc
     7261 tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc tcataggtga
     7321 ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc
     7381 gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga
     7441 gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca
     7501 gtggaacgtt tgagcaactg gattctttgt catcgctgtg gaatgagaac gtccgtcatt
     7561 gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt agggcgatgc
     7621 gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc
     7681 tttttttgga caggacagct tactgaaaaa caaacggaaa tctcaaagag aatactttgc
     7741 cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg
     7801 ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg
     7861 cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa
     7921 atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt
     7981 acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc
     8041 gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac agcgattaaa
     8101 ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact aaaggagatc
     8161 aagcagacgt ttgacatcca caaacttgct ttacgctttc atcggcgttt gcttcctgaa
     8221 cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa gaagataaaa
     8281 tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt attttgtcca
     8341 agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc gagatatgag
     8401 atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa aatgcgagaa
     8461 gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac atttgaaaga
     8521 atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc tttggtacaa
     8581 atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata ttttttctat
     8641 gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat gaatgaagaa
     8701 gggaagaaga tactgtgagg tgcagttgct gcagtaaaga tatcgtccaa aatttaacat
     8761 tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca gtttgtcgcg
     8821 cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca atccatttca
     8881 atctagagag tacgaaaaag aaagtaaatt ctatccccgt ttactgcagt gactgtcaag
     8941 aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt gtgtacgatg
     9001 aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga ctagcaggca
     9061 cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc tattgtccaa
     9121 tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc gagatgttga
     9181 aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg ctggctcaag
     9241 caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt gcagtcacct
     9301 tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca acaggacaga
     9361 ctcttttcca cgcagcagat tgtttgcaga tcgcacagcc gaaaatcatc cgaactattt
     9421 ctttggcgag atgaattaga tgtggaaatt tgatagcgat ttaatttgca aataacctag
     9481 agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc ttgattgtgg
     9541 tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa tatcgaagta
     9601 actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg ctatttcagt
     9661 gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa
     9721 gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac ttctccagat
     9781 ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg caaattcaaa
     9841 acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcaaatgc tgctgcttta
     9901 ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg tacgaaacgc
     9961 ctttcattga aaccaatgga tagtgaagaa gcagtcttgc aaatgaacat gctgggacat
    10021 aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa
    10081 gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata acaacgaaaa
    10141 cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc ttttagatga
    10201 aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca attatctata
    10261 actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag aaggaaaagg
    10321 agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat aagaaagaac
    10381 taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa atggcagcat
    10441 taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac caaaaaggag
    10501 aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca gcaaaacgtg
    10561 tattaggttt atatccatac cacgtacaat tgatgggtgg aatcgtgctt cacgatggaa
    10621 atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg ccagtatatc
    10681 taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat ttagcaaccc
    10741 gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg gggttgaata
    10801 tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc acttatagta
    10861 cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac cgccatcaaa
    10921 tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc ttgattgatg
    10981 aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct ttatatacaa
    11041 gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc gatgtacaat
    11101 caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca ttcggtttgg
    11161 ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa gcattgagag
    11221 ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa gtactgattg
    11281 ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga cttcaccaag
    11341 caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg gcgacgatca
    11401 ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt acagctaaaa
    11461 cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt ccgacaaatc
    11521 gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa agcaagttcc
    11581 acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg ttagtcggaa
    11641 ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa cgtattcctc
    11701 atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg aatgctggtc
    11761 aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat atcaagttag
    11821 gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga catgaatctc
    11881 gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct ggtatgtcac
    11941 agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa cgaatcaaat
    12001 ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc aagatgctga
    12061 cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac acacgtaaaa
    12121 acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat aaacagcgac
    12181 aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg gtcaaacgga
    12241 caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg aatttggaaa
    12301 gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact cttgcagata
    12361 tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca caagaagtat
    12421 ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt gaaaaagtcg
    12481 tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgatgcaatg gatcaattac
    12541 gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa tatcaaacag
    12601 aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc actcgaatct
    12661 ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa ggaacagctt
    12721 ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa aaacagccaa
    12781 tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg aaaaaatata
    12841 agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg agacaaattc
    12901 tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt agactagata
    12961 taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa ttcgaaatct
    13021 cctgacagaa atgcaggaaa aggtcacaag cttcaggagg tctctttgac ttagaccagc
    13081 tggaagaaga cattgcggaa gcagaagcaa aaatggctga acctggtttt tgggatgata
    13141 gcgaagctgc tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact tatgatcaat
    13201 tccattctct tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg ttgcaggaag
    13261 aacctgatgc agccatgcaa gaagaagcag aaacacgtat caatgcgcta gatgaaaaaa
    13321 tgaagacata tgaattatct atgttgttag atggtcctta tgatcgaaac aacgccatcg
    13381 ttgaattgca tccaggggct ggcggaacgg agtcccaaga ttggggcagt atgcttttac
    13441 gtatgtatac acgctgggca gagcagcatg ggtatcaagt agagacacta gattatcaag
    13501 caggtgatga agcaggaatc aagagtgtga ccctactgat caaaggacac aacgcatttg
    13561 gttatctgaa atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct tttgactcag
    13621 cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt gatgaaacga
    13681 tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca agtggtgcag
    13741 gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg cctacaggaa
    13801 cagtagtagc tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa gcgatgggga
    13861 tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa gcagctgcac
    13921 ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat gttttccatc
    13981 catattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc caagcagtta
    14041 tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta caataagaac
    14101 ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac attgccatgc
    14161 tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa tgaaggatgt
    14221 aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcaattg tcatcgaaca
    14281 aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat tcatcaaatt
    14341 gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc atgatctaat
    14401 agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg tgttccaaga
    14461 ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta tgcaagttat
    14521 cggcagaaaa cctcgggata tcaaaaaacg agtaatggaa gtgctggacc ttgttggttt
    14581 gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc aacgtgtctc
    14641 tattgctcgg gcaatagtga atacgcctaa agtacttatc gcggatgaac cgacaggaaa
    14701 tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca atgcccaagg
    14761 aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc ggcaccgagt
    14821 aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat atggatacga
    14881 tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa cgtaacggct
    14941 ggatgacagt tgcttcagtc agtgcggtaa cgatcacatt gactttagtc ggtgtattca
    15001 tggcagttat catgaacgca actaaattag ctcaagatat agaaggaaac gttgatgtct
    15061 ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa aaagaattga
    15121 atgacttaga tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa ttgaaaaaaa
    15181 tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac ccgttatatg
    15241 atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca gaagaagcag
    15301 cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat cggattttcc
    15361 aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg ctgttcgtag
    15421 cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag cgtgaaattc
    15481 aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt ttccttgaag
    15541 gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca tttggttatg
    15601 catggttttt ccaattgatc aatccatcat tattacgttc ccattattca ttgattcatc
    15661 cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg atcattggtt
    15721 cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac aatataaata
    15781 aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta ggagaataga
    15841 aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta agttggtaga
    15901 agaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct tttgattatg
    15961 aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc tcaaacatgg
    16021 gcatgggtgc tcgttttgat gttcattcca gttgtaggat ttattttata tattttttta
    16081 ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa aatcggtaag
    16141 aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc tcatccgcct
    16201 actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga aagtacgctc
    16261 tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt cgatgcgctg
    16321 attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat ttatcgcagt
    16381 gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg aggggtaaaa
    16441 gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt ttttcagaat
    16501 ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc gtatattaat
    16561 cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa aatcggatat
    16621 acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt tggttattgg
    16681 cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa ccgttttttg
    16741 atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt ttttccgtcc
    16801 attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga taccgaacat
    16861 gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga gatattgatc
    16921 caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt ggctcttctt
    16981 tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct cgtttactgg
    17041 gcaacgtatt ctttcgctgc agagctaatc gagtatggag caaggatcga aacatatgag
    17101 aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc agttggctca
    17161 gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact ggtttatgat
    17221 gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat ttctacgcat
    17281 ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga aggattagca
    17341 agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc aacagatgga
    17401 gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat ggagaaagcg
    17461 tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa gcaagaaata
    17521 agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata gaaactttta
    17581 atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga ttttgattcg
    17641 ttttcatcaa gtaa
//
LOCUS       DASJSZ010000057        17507 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.057, whole genome shotgun sequence.
ACCESSION   DASJSZ010000057 DASJSZ010000000
VERSION     DASJSZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.057"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            106..456
                     /locus_tag="U4B03_001839"
     CDS             106..456
                     /locus_tag="U4B03_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEN2068685.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            555..1085
                     /locus_tag="U4B03_001840"
     CDS             555..1085
                     /locus_tag="U4B03_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN2068686.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1239..2390
                     /locus_tag="U4B03_001841"
     CDS             1239..2390
                     /locus_tag="U4B03_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN2068687.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2499..4538)
                     /locus_tag="U4B03_001842"
     CDS             complement(2499..4538)
                     /locus_tag="U4B03_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEN2068688.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(4596..5153)
                     /locus_tag="U4B03_001843"
                     /pseudo
     CDS             complement(4596..5153)
                     /locus_tag="U4B03_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(5692..5898)
                     /locus_tag="U4B03_001844"
     CDS             complement(5692..5898)
                     /locus_tag="U4B03_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068689.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5914..6528)
                     /locus_tag="U4B03_001845"
     CDS             complement(5914..6528)
                     /locus_tag="U4B03_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEN2068690.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6675..7358)
                     /locus_tag="U4B03_001846"
     CDS             complement(6675..7358)
                     /locus_tag="U4B03_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN2068691.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7467..7925)
                     /locus_tag="U4B03_001847"
     CDS             complement(7467..7925)
                     /locus_tag="U4B03_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEN2068692.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(8009..9772)
                     /locus_tag="U4B03_001848"
     CDS             complement(8009..9772)
                     /locus_tag="U4B03_001848"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEN2068693.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9993..11453)
                     /locus_tag="U4B03_001849"
     CDS             complement(9993..11453)
                     /locus_tag="U4B03_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068694.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11661..12587)
                     /gene="glsA"
                     /locus_tag="U4B03_001850"
     CDS             complement(11661..12587)
                     /gene="glsA"
                     /locus_tag="U4B03_001850"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEN2068695.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12610..14037)
                     /locus_tag="U4B03_001851"
     CDS             complement(12610..14037)
                     /locus_tag="U4B03_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2068696.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14068..15282)
                     /locus_tag="U4B03_001852"
     CDS             complement(14068..15282)
                     /locus_tag="U4B03_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEN2068697.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(15623..15865)
                     /locus_tag="U4B03_001853"
     CDS             complement(15623..15865)
                     /locus_tag="U4B03_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2068698.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(15897..16454)
                     /locus_tag="U4B03_001854"
     CDS             complement(15897..16454)
                     /locus_tag="U4B03_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2068699.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(16467..16655)
                     /locus_tag="U4B03_001855"
     CDS             complement(16467..16655)
                     /locus_tag="U4B03_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN2068700.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(16668..17234)
                     /gene="amaP"
                     /locus_tag="U4B03_001856"
     CDS             complement(16668..17234)
                     /gene="amaP"
                     /locus_tag="U4B03_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN2068701.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         4986 a         3503 c         2972 g         6046 t
ORIGIN      
        1 attttgtcac aatccttttt gtttttttga ataatcgctt tttcttctct ttttcttata
       61 tactataagt agtaaataat ttgaaaaaaa gaaaggtgac tgattatggc gcaaaatcgt
      121 atttttacga caagttttgc aagcgtttat cctatgtatg tagcaaaagc ggagagaaaa
      181 gatcgtataa aagaggaagt agatgaaatc atttgttggc tgacgggtta ttcccaaaca
      241 gaactagaat atcaactaca aaaacaagtg actttcgaac aattttttgc ggaagcccct
      301 gtccccaatc cttcacgttc cttgattaca ggtgttgttt gtggtgtacg agtagaagac
      361 atcgaggata acttgatgaa agaaattcgt tatttagata aaatgatcga tgagctagca
      421 aaaggcaaaa aaatggaaaa gatcctcaga agttgatttt tccagagata ttcttctcct
      481 tcgctcattt tgtaacattt aatttggtaa aatgactagt tcacgttaaa ataagtcaaa
      541 ggaggttttc ttatatgttt gagacgatta aacaaaatat tcaaaaatac gccttattaa
      601 gagctgctgt ttatattatt tctgggattg cgattgtgct aaacccttct gctgtttttc
      661 attttatcgg ttatctgatc accgcctatc ttgttttgct tgggttgttg aatctttttg
      721 aggcttaccg ttttaaaagg aaaacaggtt cttggagtat cggtctgttc agtggtcttt
      781 ttttcctgat cttagctctg atcgtctatg cttttgcacc tgcaatcgtt tctatcctgc
      841 cgattcttct tggattagct gttatcatca acagcttgtt ccagttgttc ttcagcatga
      901 atacaaaagc taaaagttgg agtgtttata gtagtattct gttgttgatt ggcggattcg
      961 tcttattgtt caatccattc aaaagtttga tgcttctatt ccaaatattc ggctttattt
     1021 tgattttcat gggcatccag gagattatca atttcatcag aataagaaaa atgactatac
     1081 gttaatcatt tttagttatt aaaatagacc gtataaaaac caacaatcca aaaaaatctt
     1141 ttggattgtt ggtttttttg tgagtatatt ttcctatttt ttcaataatg tatgttatgc
     1201 taagttcggg ctatatttta tacaaaggaa tgatgagaat ggaatttatt ggtattttat
     1261 gcctgatatt gatttcaaca acgattggct cacatatctc gcggagatta ggtatcccag
     1321 cagtgattgg acaattactt gttggtgttt tgctaggcca agctggattg aattgggtca
     1381 aacctgatat tctagtccat gacttttcgg agattggtgt gttcttatta atgtttttag
     1441 ctggtctgga aagtgattta tctcttttga aaaaatattt ccgacctggt atgttcgttg
     1501 ctttgcttgg gatcatcttc ccggttgttt ttggctggtt gacaggcaaa gcattccaag
     1561 tttcgggaaa tgaagcgatt ttctttggga ttattcttgc ggcaacttct gtcagtattt
     1621 cagtagaagt attaaaagaa ctaaacgtag tgaatacaaa agaaggatca acaattttgg
     1681 gtgcttctgt ggtagatgac attttagtcg ttttagttct aagtttcagc ctatcttttc
     1741 taacggggga agcttctagc aatgcttctt taccgcttgt tttgattgaa cagctaattt
     1801 attttgtttt tattttctta ttagtcaaat ggattgcgcc tttcctactt tcatttgcag
     1861 aaaaagtgta cgcaaattct tctattatca tcgtatcgct agttatttgt ttaggaatgt
     1921 cttacgtagc ggatcttgtt gggcttagct ctgtcattgg tgcttttttt gctgggatag
     1981 ctgtcagtca gacaaaggtt aaagaagaag tataccacaa tgtagaggct ttaggatatg
     2041 cagtatttat accagtattt tttgtcagtg taggattaga agttgatttt tctgacttta
     2101 gtgaacaact actctttatt tctatattga cgcttgtagc gattttgacc aaattgcttg
     2161 gaggttattt tggtgctcag attgccggtt tttctcaaca tagttcattg atggtcggtg
     2221 ctggtatgat ttcgcgtgga gaaatggctt tgatcatttt acaaattgga cagcaaagtc
     2281 aattgatcga accacagtac tattcacctt tagttatcgt gattttatta agtacactta
     2341 tttctccact tgttctcaaa tattttacaa aaaaagttta tgaagtgtga gtaaatcaaa
     2401 aagaagttgt gaaagagaca tcgtgaaaac cgtttatttg aaaaaaggac tgtgacgata
     2461 acgccacagt ccttttactg tacttaaaaa gcaatttttt attgataatt ttggttgagg
     2521 tattgcaata gttccgatgc tcgtttggtt gctgaatcat catcatcttt attttccaac
     2581 atgctgacca tcatataacc ttggttatca agattgaaag caaacaagaa actgttctct
     2641 tgccccttta catcctgttt ttctttgatt tccgctgtac cagttttcgc tgccaacgga
     2701 atccctaaag cagaaagaga atgtgctgta ccatttacat cttgcacaac ttctctcaga
     2761 tctggcacga tcgtttgtac tgctgtttcg ctgatgacat tcttcttatc ttttgtctct
     2821 ttatctgcaa tcaatttagg atagacaagt gtgccattgt tggtaaaaac agaatacatt
     2881 gctgcttgct ggataggagt aattagaagt tcgccctgtc catatccagt atcagctagc
     2941 aagatatctg agttaaagct atcttcatta gaaatttgtg ctggattcat actaattggc
     3001 aaatcaaggt cttcaccaaa aatgaattta tctaagcctg tacgaaattt tttctcaccc
     3061 attttcaacg tttcttgtgc cgcatatata ttatcggaat agatcaaagc agtttttaag
     3121 tctacttgtg atacatcact actgacacgc gttgttttaa aagatcccca agaactgtct
     3181 ttttgccatt taagcccgtt gatcgtcaac acttcatttg gatcgatagt gccgttgtcg
     3241 agaccgattg ctgctgtaat tattttaaac gtagatccag gagcataacc tgtcgcaaat
     3301 cggctgatga atggatgttc aggattttct tcataagctt tgtaatcttc ttgtgagatc
     3361 ccgtttgtca ttttgtttgg atcatagctt ggagagctag caagcgcaag aagatcaccg
     3421 gtttttggcg ttgtcgcaac agttgatcca gcttttcctc ctagactgtc aaaagctgtt
     3481 ttttgtgcct tcgcatcgat tgtcaatttg atatctttgc cattttgaac ttcatgttcg
     3541 atcagaactt ttttctctac accgtctgca tctgtaatgc ttaatttctc acctgttgtc
     3601 ccacgaagat ccttatcaaa agccatctcc aaacctgagc gaccgatttt tccattactg
     3661 cttaattctg gattcttatc aatatcttct gctgtgatat ctcccacgta accaatcagc
     3721 tgagcagctg cttcacccaa aggataatat ctgccgtcta cttcttgaat ggtagctcca
     3781 gctggaagtt ctggcgttgc tccatcaatg attttcaatg ggacaaagta atcgggttgt
     3841 acccagcttt gtgaaatcgc ctgattgata gcatcttctg ttaagtcgaa ggaggaagca
     3901 atcgctttga tattggctgt tttttcgcct ccatctccaa gtttacttgg aacgactcct
     3961 aattgtttta gtttgcctgt tgttgctaat ggttcaccat ttcgatctat aatattccct
     4021 cttgctgctt cttgtgtggt caggcttact ttgtcatttc cttccatttc tggaaaaacc
     4081 aagttgggtt gccagttgat cgtggtttga ccatcatttc tgtctaatgt tcctttataa
     4141 gaaagatctt tcaattcacc taatgaggta ttcatctttg ctttatatga aaaactgtaa
     4201 gtagaatcgt cttttttatc tacttttaga ttcgatatct gaagtccttt gacatcagca
     4261 gcaccgtata tattctggta ttgatctaag atctcttttt cagataatgc acttttattt
     4321 gccgcctttt ttgatgtcat ttctgcagct ttgttataat ctcccttgtt caaagcttgg
     4381 acaaattgct caactgtctt ttctccagct tctactgctt gggtttcttg atagtgctgg
     4441 tagtaaaaat aaccgccacc cgcagctgct attaagatca ctgcgccggc aatataagcg
     4501 cctgttcgat ttttgccgtg cttgtcactt cttttcattt tttcctcctt ctcttgcttg
     4561 tcattccttt atttcactat ttatacctgt ttattctaac atatgtcttt gaggaaatcc
     4621 ataccacaat ccagaagcac actttctgga aggttcgttt ggatactacc aagtaatttc
     4681 ccaaccgctt tgggtagctt gaatattgaa gttacgtctt tcagctgctg agagatagct
     4741 ttgatcactt gctgctgcct tctgatcctc ccgaagtctc cttctttgtc ttcccgaaat
     4801 cgagcatact ggagtaaagt attaccatcc atcacttgct ctcctttgtt tatatatacg
     4861 ccatctaaat ctagagattt ttctgcatcg atcttgactc caccaggaaa cagttcatta
     4921 acgcaatcga taaaatcttg aaatgtgata cttgcataat agggagcttc aaatttgaaa
     4981 ttttccttta atgtttgatt taataaatca actcccccat aagagtaagc tgcatttatc
     5041 ttattcattc cgtaacctgg aatatcgaca tatgaatctc tcatgataga gatcagtttt
     5101 gcttgttgtg ttttctggtt gtattgtgca accagaagtg aatccgagcg acctccacca
     5161 atcgatcttt tttcaccgca tctcctcctc acacagcaat ccacttgtca ggtgaggagc
     5221 gagtttgtct catcaaataa gtcagtcaca aaaatataaa gaaaaggtca gaaacctttt
     5281 cgaacaagaa ttctgaccta gcatcatctc atcgtccgag ttttagcact atacaacgta
     5341 aagaaaacat tcttagctat tggaaaaagc cttaatccaa cagtttctgt tttacccatt
     5401 ggcgactttc gacgtttctg ggcaacagcc tatgtttgta taggagcctc acctaacaag
     5461 gttattacta tattctattt acgagttcat gatagacttt tcctaatgat taggcaatgg
     5521 aatttgaaat gaaatagtga aatttaacaa tctttaatat tttttagaaa aaaataacaa
     5581 ataagttgaa agaatcccgt tgcaaattct ttcagttgca ccaggattct ttgataattt
     5641 tttagtttta tgtgtttttt tatatgtttt ttcacttttc ggaataaata tttatttgtt
     5701 aaagtaatta aacaatccgc taatcgtttg atctggcatt tctatttcct taccatctaa
     5761 agtaacagta gtaacgacaa aatggtcttg caatggcgga acttctaagt actgcccttg
     5821 cagaaaatca ttcactgttt catattcttt tttcatttct tttgaatctg tcgatggttt
     5881 atagcttatt tttaacataa ctagtgctcc tttttattcg ggacgtgcaa tcgtgaaaat
     5941 ttctcctatt gctgcataat catttcctgc caaacggctt acagcattca attttcttgg
     6001 atcgatcctt cctgtcttat catcatagac tgtttcattt aaatgatatt tctgtacttc
     6061 tagcaaaaat aaatcagctg ttactttatt tttattccgt atctcgacat gttcatataa
     6121 ggatgtttca aaacgaactt ttgcttctgt caatgctggt actgaaatga ttactgaatc
     6181 agttaaagtc aagtcagtca ggtacagttc gctttcctca ttaggcagat tggctgctgt
     6241 tttatttact tcttctacgt tgtcctcatc aaggatatgg ataacagctt ctcttgtctc
     6301 aagaatattc tttgctgtgt cttttactgc atcttgtttt ctctggatcg ccaaggataa
     6361 gatgggtcga ttcgaactga cgatattgaa gtaactgaag ggtgcgatat tcactatatc
     6421 ttttgctgat ttagtcgtta ctactgctac tggtctaggg atgactgaac caatcagcaa
     6481 cttgtaattt tcacgttctg tcaattctga aggactccgt tcaaacatgg tttcgccttc
     6541 tttctataaa attcgttaaa aagcagatct tgaagacaaa atgaaaagta atgctttttt
     6601 catcttctta gaatctgctt taaactatct atcaattttt cttttttgtc aagaatctgt
     6661 gccgtgcgtg ttttttagtt atttgcttta cttgcggctt cgacatatct tttagtgatc
     6721 aattgcggac gggtgataat agatccaact acgactgaaa aagcacctaa ttcatatgct
     6781 ttctctactt gttcaggaga gtcagtatgt ccttccataa ttactgggat atcgacttgt
     6841 tctatgattt gattcaataa atcaaaatca ggtgtctgcg tctttcgtga atattctgta
     6901 tagcctgcta aagtcgtaga caaaagatca aaaccatctt cttgcgcttg aagcgcttcc
     6961 tcaaaagtcg aaatatctgc catagctaac cgaccgtgtt cgtgaatcat ttggacaagt
     7021 tcagcagttg ttttttctgt cggacgttta cgagaagttg catccagtgc aatgatttct
     7081 gcttgcgtct ccaaaagttc ttccacttct ttttctgtcg cagtaataaa cacttctgac
     7141 ccatcgtaat ctcttttgat aatccctatt accggtaaat caacgattcg cttgatttca
     7201 ttgatatctt ctgtcgtatt tgctcgaata ccaactgctc cgccttcttt tgcagccaaa
     7261 gccattctcc ccataataaa agaactgtgt agcggctcgt tttccaaagc ttgacaagaa
     7321 acgacgactc cgcctttgat ttgttccagt gtatccacaa tatctcctcc agtaatgtta
     7381 tataaaaata cagcttcata taaaaatgta acccttttct attcaggaga caataatgag
     7441 ctatttcttt cagcagacct ttcttactaa aagaaaatta cttcaaattc ttcgtatacg
     7501 accagcttct cttctgtaaa ttctatgttc aacgcttctt tataagcatg tttaaaaaac
     7561 tcagcatgga ctttccgcca ataagctaat gtccgatccc cttctccttc tttccaagca
     7621 tgctctttcg tgacttcact aaaaggcaag atggtcacat ttatattttt gatgatacaa
     7681 actggttcat tttttccatt caggaccacg tctaattgac caacttttgg taaaggctca
     7741 ttctcttgtt tatacagatc gtaaccactt gatgtaccag tctttatctt ttttacaact
     7801 agttcgctca actcgtctgc cattttttct gtatccccaa atgaaaagaa acctaatttt
     7861 tctgattgat aactaggata tttttcttgg aactttttga catattcttc tacttgctga
     7921 tacataacat ccaccccttt gctttttata atgaaaaaac catccagata ctagtataaa
     7981 caattatctg gatggtgagc ataatgaatt attgaatatt caagacacgc agactatctt
     8041 caacgatttg ttttggtgtt aaatgataac gttcataaag ctgccctaca ggaacgctat
     8101 ccgtgaattc tttctctgca ccaaaattta atacgttcat tgaagaatca ccataataac
     8161 gactgatatt ttcgccaaat cctcctgaca aactaccatc ttctaatgtg atgactaatt
     8221 cgtgtgtttc ttttaattta tctaatgttt ctacgtcatt ttctgtgatc gttcctggat
     8281 tgatcaacgt tgcttcgatt cctttttttc taaaagcttt tactgtttct tttcctaatt
     8341 caagaaaacc gcctaaagct aaaattgcta cttttcctcc attatttact gtttgatagg
     8401 ctggaatact ataatcattt gcgatagctg taccatgttc tacaccatgt gtaggaattc
     8461 ggattgctac aggctgcgct gtttgttcga acgcccaatc tagcatagag atcatatcct
     8521 cttttgttgc tggagcaaga tatttgatgt tcgggatact tgataagtaa ggaatgtcaa
     8581 atgttccttg gtgtgttcgg tcactagctg agatcgttcc gccaccgact aaaataatca
     8641 ctggattctc gttgatcgct aaatcatgtg ataattgatc atatgcccgt tgcaagaaag
     8701 tacttgattg gaagacgact ggacgtactc cctgagaagc caaagcagaa gcgaaagtga
     8761 tagagtgctg ttcagcgatc cccacgtcaa aataatgatc tggatgtttt tcttggaatg
     8821 ttttcagtcc aaaggcgcct ggtatcgctg cattgactgc ggcaatagga gctgtacttg
     8881 tttgcatttt cttatccaaa taatccatga tgatcatatt aaatgtttct ccagaagtta
     8941 cttgctttgc ctccccggtg gacagatcaa atggtacatg ccaatgatat ttcattttat
     9001 tatcgattgc cggctgataa ccgcgacctt tttccgtatg gacgtgaagt acaatcggat
     9061 gatcaatatc ttttacttct tcaaataaac ggatcaccgt ttccagatcg tttccattat
     9121 ccacgtaacg ataatcaaat cccattgctt taaataggtt attgctgctt ttaccgttgg
     9181 tttgacgaag ctcagctaag tttttatata aactgccatg attctcatca atagacatct
     9241 cattatcgtt tactaatacg attagattgg attgtaaaac accaccattg ttcaatcctt
     9301 caaaagctaa gccaccactt agggagccgt caccgataaa tgctaccaca tttcctgatt
     9361 ctttcttcag atctctcgct ttagctaaac ctgttgctaa gctgatagat gtagatgtgt
     9421 gccccactgt aaaatagtca tgtttacttt catgttggtt ggaatatccg ctcacttcac
     9481 ggtaatgtcc tttttcaaaa ccattttttc gaccagttaa aagcttatga ccataagact
     9541 gatgggaaac atcccaaacg attttatcct ttggtgaatc aaatacataa tgaaacgcaa
     9601 tcgttaattc tactacacct agattcggac caacgtgccc acctacatcg ctgtcctttt
     9661 gcaagatttt ttggcgcatc tcttctgcca actgtttcat ttcctcaaca gataattttt
     9721 tgacatctgc ggggctattg attgcatcca atacatgtaa tactgtactc ataatacatt
     9781 ctctcctccg tactatcttc ttttaatgaa aatagatttg ttacacttta tctattctac
     9841 tacttagagt tgactctaag tcaatagcat tttaaaaaaa tttcagagaa agcttgttat
     9901 ataagtagat cttcgcacga atcaggactc aaataaaaac cttctacaga caattaaaaa
     9961 gctttgtaga aggtctccta attaaggtct catcaagaaa tatgtttttt taataggtga
    10021 agttcctgag caatatgata caaatggatg tttgcacctt ttaatgagat tggatagatc
    10081 tttgtctttt cagtttctaa tccattttga tagctagcag catcgcacaa aaccactctt
    10141 tttttcgcac atagacattc atcaggcaat tcttctaatg aacgaaataa aataggattt
    10201 atttcagcga tgccttcttc cgcatacaaa tgaaattggg ttcgataaaa aaattgtttg
    10261 actgaacttt ctgaaacaac aaaaatttct atttcctgtc tccccgcttg ttcactgttg
    10321 atcaaagaaa gacttttgac gaattttcga atgaaatttt cattccatct tagttggatt
    10381 tgatttttct ttttccactc ttccaatatt tctacaattt ttccgtacag aagttgttct
    10441 ttttctgata gaagatagat cctttccggt aaaaatactt gtccatctgc ccacaacgta
    10501 cggacaaatt gtaaaaagga ttgcttgaaa ataaaatttt ctgcatcgtc gatttcagcg
    10561 atactaataa tctgtaaaat caattcattc agtgataggt cattgagaac aaaattttta
    10621 taaacaactt cgaaatcttt tcttaacagc tccatgttct cattggtata atttctactg
    10681 ttgaacaaag aaaaaatata atagacatct tcttcttgta agtctagaat tcctttttgc
    10741 tcttccaatc cttgacagac taattgaaat aacggcttcg tttctgcgct tttcttatct
    10801 tcttctttca gtccaagggg aaatgccttt cttccgattg cgatctctat tcctcgtatg
    10861 ataaaggact tttcatcttc cgaatatctt cttttaaaaa aatgagtctc tacataatca
    10921 atcaactcgt tacaagcttc ttctacatac ggatttttat tttcatagcc ttcccaattg
    10981 acataacgta ctaaagctag caagatgttc cgcgagtcaa attctgggat aatgatttct
    11041 ttatttttca aataatcatt ttctttaaaa aaatccaata cgattttttt gattgtatat
    11101 actttgctca aagaaataaa ttcaagatca gccagttctg tactcgtgaa tataccatta
    11161 aaataataac ttaaaaactt taaaaaataa gattgaccgt acagaaactg tgtgagctca
    11221 gaaagagcaa taccatgttt gaatacgagt ttatacttat gcttctctct tacttgttct
    11281 tttatacaat attctagaca ctcggctaga ctttctagat cgtgaaggat cgttggtgta
    11341 gtcaccctta gagaaatagc tgtttgttcg atgtctattt cttggaattc aaataattgt
    11401 tcacacagat acacttttct aaatatattt cgctcgatgt aattttccag cattcctgat
    11461 acacgcctcc agtttttctt atgattttat ttaatcatat ctgtaaatcg tatttttgtt
    11521 tcctttttat ttagaaaagc cctttcaaaa accaataaaa aaagaaactg ggacaaaagt
    11581 caaacaagct cacaatttca gcctcattgt aggtagcttt gagccgtttg gacttttacc
    11641 ccagcctctt tcaagaatgg ttattcaaag atgtcagcgt ctagttcttt gactacatct
    11701 ttcaacaatt ggataccagc tgcgctgtta ccaaatggat ctagtgccgg gctgaagaca
    11761 cctatgccgt aacgatttgg cacagctgcc atcaacccgc cgcctacacc gctttttgca
    11821 ggtacaccaa catggacgga aaactctcct gattcatcat acaaaccagc agttgtcata
    11881 attgatttga cgattgtagc actttcttca gtaatgattt gtttgccatt ccaaggagca
    11941 attcctttat ttgccagcac agcagctaat tttgctatgc ctgtagcggt gacattgagg
    12001 gagcattgtc tgaaataagc atcgagtatc tccggtacgt ctccttcgat catttgatta
    12061 cctttcatat agtaagcaag agagcgattg atatcacctg ttcttgattc agaatgatag
    12121 atttcttcat tcaatgtgat attaggatca tcacagatct ctttcataaa tgacaagatt
    12181 cgctcaaatt tttcatctgc atctttaccg gcaatcaacg aggttgttgc aatcgctcct
    12241 gcattaacaa aaggattcat tggatgtttg cgatgattga tttccatatt catgatagaa
    12301 ttgaaggcaa aaccggttgg ttccgtgttg attgtttgaa agactttttc tattccgttg
    12361 tctttgattg ctaataacaa tacgggaact tttgacatgc tttcgatggc aaagcgcaca
    12421 tcggcgtctc ctccctcaat cagttgtttt gtatccaaat cataaatcgc catacctaat
    12481 tgtttgggat ccacttcagc taatgctgga atgtaatccg ccacttgtcc tgtttgataa
    12541 tagtggaaat tttttccaat caattgttct aattctttta cttccatttg aataaccagc
    12601 tttcattttt tatttttctg catgctggaa tttatgacgg catgcgtaga tcgtaaatgg
    12661 aataactaag acgataagat aagaaacaat cagaatcgtt tcataaatcc catgctgtcc
    12721 agctgcgata ctagcaggtg gaacaaatga gatacaaaaa gcaaaaagtg aagtcaaaag
    12781 acctaaacaa gcaacgatta cttttacaac ttttccgcct ggaacctgat aggctctttc
    12841 aagactgtca tctttaaaaa ccagcttcaa atagcccgcg aaaaacataa agtacaacat
    12901 caggtagatt acaacagtca atgacatcgc tgcaagaaat gaaagattat tccccccgcc
    12961 gcttaatgta aagacggctg cccagattgt tgcaacgatc ccatttgcga tcatcaaatg
    13021 agcaggaaca ccgtgtgtgt tttcttttgc aaaatattca ggaagtaatc cttcttctgc
    13081 tgctacgtga agtgcttttg aaggagaggt tacccaagaa gcaatttctc cgatgacacc
    13141 taaacaaaca agaatcgcaa gaattttcgc aatccatgca agatttggat caatgtgtct
    13201 taataggaat tctaacgatt gaatcacccc tgtattaaga gacaaaccat gttgaggtac
    13261 tgtagtagcg acggtcaatc cgccaaaagt atctaaaatg attgccagaa taaccaatac
    13321 aaacattgct aatggataat cctttttagg gttttccatt tcatttgcat gactggcaga
    13381 tgtttctacg cccatatatg ctaaaataaa ggaaacaaaa acaaccagcg taggcaattt
    13441 cgaaaaatca ggaatcaatg ttttcataga cagtatcgtt tgtagtggag cgcctgaagc
    13501 gacataatgg atccctaagc caaacaagac taatgctggc aacaagatac cagctacaaa
    13561 accgatccga gcaatcaatg aagtgtattt ggttcctttt aactgtgaga gtaaaatagc
    13621 ccagaaaata atcaatagga ttgccaattt cagatattta ttgttttgta tagcaccaat
    13681 acccgttgca taggataatg caccagttaa gaaatacagc atggtcaagt agccaactgt
    13741 tatctcaaac cattggaaaa agattgcggc aaaaccgaat cgttttccca atgtgcgact
    13801 gacccaagta tagacaccgc ctttttccca tcctttcacc gttgccatct ctgctgcgca
    13861 caatgccaca gggataaacc atagcaaacc gcctaataac aagaaaaata ctaagctgaa
    13921 gcctgaggtc gcgaatgtgg gatattcata taatgaaacg accattgaga tggtcatagc
    13981 caaaaagcca aagaacgtta atttttttgc aggaattttc gttttgcttt gttgcatgaa
    14041 tcttcctctt ttctatttga atttgtttca attgagtttg ttgccaaaga agaaaaagtc
    14101 caatccttct tcttgttctt cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt
    14161 taaaatcact gttgtcagca cataggcata cacggcagta atgatgacac caatcacttg
    14221 aatccctaag aagtaccagc tgcttggatt cgttagtaaa tggctgttag atgcaaagat
    14281 ccctatcaga atcgtacctg tgaatccgcc catgccatga acaccccaca catcaagtgc
    14341 atcatcccat tgtttctttt tacgaaaatc aacagcagca tgacagacga tacccgcaat
    14401 gatcccaatc agcatggcac tttttgcttc tacataaccg gcacaaggcg taatggctgc
    14461 caatccagca acggatcctg ttagtacgtc gacaaaatca aagccatttc cttttacttt
    14521 tgcaatgatc agccaagtaa tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc
    14581 attgactgct tgttcatctg ctcgaagtgc accaccggaa ttaaaaccga accagccaaa
    14641 ccaaagcagt cctgtaccga tagcagctgc cataagactg ctatgctgac ttctttctcc
    14701 taaaggaatc tttctttgac caagtgtgag gatacatgct aaagagccga atccagctgt
    14761 cgtgtggatg actgctccac cagcgaaatc tctaaatccc atctgatcca aaaatccgcc
    14821 accccatatc cagtggcaaa cagggaaata aatcagcaga ttccaaaaaa taacaagtat
    14881 aatatagcct tttaaattca atctccctgc aaaagctcct gtcatcaaag gaacggtaat
    14941 cacgataaac atcagttggt aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa
    15001 catgttgggg aagcttgtaa catgtctcat caaaaagaaa tctgccgggt ttccaataac
    15061 accgccgata tcccgtccaa aggccaatcc gaatccacca aatacccaca taatcgttgt
    15121 tatcccaacg gagagaaaag attgagacat gatcgttagt atattattct tccgtgctaa
    15181 tccgccgtaa aaaaaagcta gaccaggagt catcaggcaa accattgctg tacataaaat
    15241 catgaatgct gtatctcctg tattgatagg taatgtattc atttttttcg cctcctttct
    15301 acgattacga gtataaaaga aggtgtagtt gtttttttct tctgtttttt tgaattaata
    15361 cttgcattta taaaagacag acaattaata aaagcttgat gtgttgtgct ctttttcgtt
    15421 tttcacacaa ataaagacaa aaaataaacg gtcttctttt tatagaaaac cgtttatttt
    15481 cgtctttttt tatttttaaa gatatttttt cataaggaat aaagaatact tcatataaaa
    15541 tatataaaaa agaaccgcag cgagtgtcgc tacggttctc aaatttgttc ttcatttcaa
    15601 cataagtttt tcttcaattt gcttataatt tacgtgcaaa gaatgaaacg actaatacta
    15661 aaatgattgc accgatcaat gatggaatga tagccattcc tgcaattgtt ggtccccaag
    15721 ttcctagtaa agcttgtcca attgctgaac ctactaaacc agcaatgatg tttgcgatga
    15781 tccccattga tgatccttta ttcgtgattg ctcctgcaat tgctccaatg ataccgccga
    15841 cgattaatgc ccaaataaat cccattttaa attcctccta actgacttct tacttcttat
    15901 tgtacacgtg gttcgttatt ttctttgact ttttgtgcac ctttgcctac agcagatttt
    15961 gctttatctg tttggcgtga agcaaattgt cctgtggatt ttgctgcttc tgttactttg
    16021 tcttgtactg tttcttgatc tttttggtat tcttcttgtg ttttgatgtc tgcaacattc
    16081 acattcactt cgataacatc taaatgtgtc atattgttta cttcagtact gatcaattct
    16141 ttgatttggt tgtaaatatc ttcgatgtct ttaccgtatt ctacgatcac atctagatcc
    16201 acagctactt gtttcttacc tacttctgta ttgatacctg ctgttacgtt atctgtgttg
    16261 actaattttt cagccatatt tgagaaaaat ccgccatcta atgtcaatag accatctaca
    16321 tctttcaatg cataaccgat gattttttgg atgactttgt cttcataagt caattcacca
    16381 ttcatcatgc ggttttctcc tttttcattt gccaaaactg atccatttgt tgtattcatg
    16441 tttttgtttt ccatctttgt ttcctcctat gatttaaaaa attgatcaac tattcctgac
    16501 tgtttcaaat aaaaaccgat tcctgcgcca attgctgtca tgattatgat caacaatgtc
    16561 ttgaaaaatc caatcgttaa ccataaaaca gctagcagta gtccaagacc cccaaagata
    16621 atagggagct tgtatgctga aaatagttct ttcattattt ggccctccta ttccactcga
    16681 gagtgcttgt agtctcttgt atcttcttgt tcttcataac cgctgtatgt gactgccact
    16741 ttgaccgttt cttttgttcc gagtacttta gttacttctt cttcgatatc ttgcatcaat
    16801 acacctgttt tccctactaa tgaagaagtt cgtttcaagt ctccgttgac atggatatgg
    16861 attcgatttt tcgttgaacg tatccgtact ttaggtgaat gaatgaactc ttcttcgtgt
    16921 aaatgggaac gtaccaaccc ttcgatcgct tttttgtcga ttgttagttt gccatcttcc
    16981 cgtttcataa cgaaagttcc tcttgacttt ggatagaaaa gaacgactag caagatgata
    17041 atagcaagaa cggcaaatac tacagctgcc cagaaaatat attgttgcat gtagtaattc
    17101 gttaaagtaa agaagcgaaa tcgttccaat tgttcaggca taatatacgg cgcattgatc
    17161 aacgcaatcg ctagaaatac tgaaagtagc agtaaactca tgataacacc gattattttt
    17221 aaccccttat tcattttcca cctcctataa acttattttt accctgtaaa taactctacc
    17281 gacaatcaca gtatagatag cgaacagtta gcatagcatc agtaaataaa gcgctaccaa
    17341 acattgctgg atcataacgg cgttttattg ccagtctgat actgaaataa caattgctta
    17401 cttattctct atttactttt gattgctggc tatacttaaa ggatattatt caagattaaa
    17461 aattgcaaag attatgctca gttattatta caagattcta tttattt
//
LOCUS       DASJSZ010000058        17007 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.059, whole genome shotgun sequence.
ACCESSION   DASJSZ010000058 DASJSZ010000000
VERSION     DASJSZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.059"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..126)
                     /locus_tag="U4B03_001857"
     CDS             complement(<1..126)
                     /locus_tag="U4B03_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEN2068702.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="U4B03_001858"
     CDS             complement(230..541)
                     /locus_tag="U4B03_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2068703.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="U4B03_001859"
     CDS             complement(543..740)
                     /locus_tag="U4B03_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEN2068704.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="U4B03_001860"
     CDS             complement(1134..2861)
                     /locus_tag="U4B03_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEN2068705.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="U4B03_001861"
     CDS             complement(2854..4047)
                     /locus_tag="U4B03_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2068706.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="U4B03_001862"
     CDS             complement(4234..4878)
                     /locus_tag="U4B03_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2068707.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="U4B03_001863"
     CDS             complement(4972..5106)
                     /locus_tag="U4B03_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEN2068708.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="U4B03_001864"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="U4B03_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEN2068709.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="U4B03_001865"
     CDS             complement(7237..7710)
                     /locus_tag="U4B03_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEN2068710.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="U4B03_001866"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="U4B03_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEN2068711.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="U4B03_001867"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="U4B03_001867"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEN2068712.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="U4B03_001868"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="U4B03_001868"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEN2068713.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="U4B03_001869"
     CDS             complement(11757..12896)
                     /locus_tag="U4B03_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2068714.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="U4B03_001870"
     CDS             complement(13205..13918)
                     /locus_tag="U4B03_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEN2068715.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="U4B03_001871"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="U4B03_001871"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEN2068716.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="U4B03_001872"
     CDS             15107..16639
                     /locus_tag="U4B03_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2068717.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="U4B03_001873"
     CDS             complement(16830..>17007)
                     /locus_tag="U4B03_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2068718.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5324 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DASJSZ010000059        16890 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.060, whole genome shotgun sequence.
ACCESSION   DASJSZ010000059 DASJSZ010000000
VERSION     DASJSZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.060"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..571)
                     /locus_tag="U4B03_001874"
     CDS             complement(59..571)
                     /locus_tag="U4B03_001874"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEN2068719.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(585..2882)
                     /gene="recJ"
                     /locus_tag="U4B03_001875"
     CDS             complement(585..2882)
                     /gene="recJ"
                     /locus_tag="U4B03_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEN2068720.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3024..3467)
                     /locus_tag="U4B03_001876"
     CDS             complement(3024..3467)
                     /locus_tag="U4B03_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEN2068721.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3468..4256)
                     /locus_tag="U4B03_001877"
     CDS             complement(3468..4256)
                     /locus_tag="U4B03_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2068722.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4253..5194)
                     /locus_tag="U4B03_001878"
     CDS             complement(4253..5194)
                     /locus_tag="U4B03_001878"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEN2068723.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5371..5958)
                     /locus_tag="U4B03_001879"
     CDS             complement(5371..5958)
                     /locus_tag="U4B03_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEN2068724.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6179..7486)
                     /gene="obgE"
                     /locus_tag="U4B03_001880"
     CDS             complement(6179..7486)
                     /gene="obgE"
                     /locus_tag="U4B03_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEN2068725.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7780..8790
                     /gene="gap"
                     /locus_tag="U4B03_001881"
     CDS             7780..8790
                     /gene="gap"
                     /locus_tag="U4B03_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN2068726.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8875..9810)
                     /locus_tag="U4B03_001882"
     CDS             complement(8875..9810)
                     /locus_tag="U4B03_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2068727.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            9996..10955
                     /locus_tag="U4B03_001883"
     CDS             9996..10955
                     /locus_tag="U4B03_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEN2068728.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(11041..12135)
                     /locus_tag="U4B03_001884"
     CDS             complement(11041..12135)
                     /locus_tag="U4B03_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN2068729.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            12324..13085
                     /locus_tag="U4B03_001885"
     CDS             12324..13085
                     /locus_tag="U4B03_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2068730.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(13200..13640)
                     /gene="fabZ"
                     /locus_tag="U4B03_001886"
     CDS             complement(13200..13640)
                     /gene="fabZ"
                     /locus_tag="U4B03_001886"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN2068731.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            13860..14615
                     /gene="fabI"
                     /locus_tag="U4B03_001887"
     CDS             13860..14615
                     /gene="fabI"
                     /locus_tag="U4B03_001887"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEN2068732.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(14725..15738)
                     /locus_tag="U4B03_001888"
     CDS             complement(14725..15738)
                     /locus_tag="U4B03_001888"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEN2068733.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            15903..16823
                     /locus_tag="U4B03_001889"
     CDS             15903..16823
                     /locus_tag="U4B03_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEN2068734.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         4914 a         3640 c         2961 g         5375 t
ORIGIN      
        1 gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt ttttctgctt
       61 aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca tggagatcca
      121 tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg atcagttcga
      181 tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc acacgttgac
      241 ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct aatccataag
      301 tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca aaaccgactc
      361 ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc ccaactacca
      421 tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc cgataagcgt
      481 caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac ggatagtcgg
      541 gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt tgttaaccat
      601 ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat tttattgagc
      661 cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc gactttttgt
      721 aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat cataaaaatt
      781 aataattttt cagggatacg caaatattga gctattgctt ttattttatg acgtatatca
      841 attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct tgtgcctacc
      901 ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt gaatgaataa
      961 gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac aactagagag
     1021 tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa aacaatcgca
     1081 gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc taaataaagg
     1141 ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc aaatacttgc
     1201 agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc gttccattca
     1261 ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc ttcttgagta
     1321 ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac aaatttcaga
     1381 tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg aaataaaaac
     1441 tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc aattcgctct
     1501 actgttactt catttggcag caatacttca tcaattctca actcaggccc tctcatcaag
     1561 tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt gacattttca
     1621 cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa taaatctcgc
     1681 atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga tcctttagct
     1741 gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt cattattttc
     1801 ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag atggatatga
     1861 ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat tgcttttctt
     1921 tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc atcaaacgtc
     1981 gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt tagtctggga
     2041 gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc gctttcttca
     2101 aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc taatgaaaca
     2161 aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat cgctactaaa
     2221 tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa tgctactccg
     2281 actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg aacaatcgca
     2341 taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac gtctacacca
     2401 gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac agtgatgatc
     2461 aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc atacccatgc
     2521 tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag tgtttctttc
     2581 attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag gattttttct
     2641 ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg tatcagatac
     2701 ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt ttcctcatgg
     2761 taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc acggatcatt
     2821 tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt tgcttgtctc
     2881 actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa aagcagaaag
     2941 aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg atcttttgta
     3001 cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt gggcgattga
     3061 gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc ttgtaactcc
     3121 gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg ctgttcttct
     3181 tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc ctgagtcagc
     3241 tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa tacaatcagt
     3301 gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa ggaagtgaag
     3361 ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat aatcaaaact
     3421 aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta ctttttgtta
     3481 aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa ttttgatgca
     3541 acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt gcggacgatc
     3601 atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa atagcttccg
     3661 cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac tgtcgtaaca
     3721 gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag gactgcaaat
     3781 ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat agaggcgata
     3841 ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg tgtcaaaacc
     3901 atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc gaatgtaagg
     3961 aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg cccgacttct
     4021 tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc taccttgaaa
     4081 gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc tacaaatgcc
     4141 attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata acagatttgt
     4201 gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa attcatgcct
     4261 cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc gtcgtgtttt
     4321 caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa tatctcgcac
     4381 tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca gcagcttgat
     4441 gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta aatgtgcttt
     4501 catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt tttctcgtat
     4561 cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct tttccattga
     4621 tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg tacagtggtc
     4681 cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta tgatcatgtt
     4741 caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta taaacagaaa
     4801 attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc agcggataac
     4861 ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc ccttttggcc
     4921 cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg agcccgggaa
     4981 gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt tttctcggac
     5041 gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac aaccaaactt
     5101 cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt tttgcaggaa
     5161 cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc tttaagttcc
     5221 tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat gggttcaaat
     5281 taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa aacagcagcg
     5341 aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac tttcattgtc
     5401 gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt tcgcatgatt
     5461 ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca agcgagtaat
     5521 ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct cttcatcagc
     5581 aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt cgaacacggt
     5641 ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc cggcaaacca
     5701 tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga ttaatccacc
     5761 cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga attgatgtgt
     5821 cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac gaacagcatg
     5881 taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa aatttaaaat
     5941 gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt tattttacct
     6001 ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag ggctgtgaca
     6061 aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg atcacttctg
     6121 tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat ttgcttgcct
     6181 attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct ttcgcaccgc
     6241 gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa cgcatcactg
     6301 tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca ccagataaga
     6361 tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga ttgaatccat
     6421 agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc gttacttcta
     6481 acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact ccagaaattg
     6541 gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc tgttctttga
     6601 atttcactag attttccggt gcatccggca tatccatttt gtttgccacg atgatttgcg
     6661 gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca agatagtctt
     6721 cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt actcgtgttc
     6781 tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct ccttcaatca
     6841 agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc attcctagat
     6901 taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat gaaataacgg
     6961 atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct gccaatactt
     7021 tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca atttccggtg
     7081 caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca ccttttgcca
     7141 caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc tccgcatctc
     7201 ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga ccgtgcatcc
     7261 ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac cgaaaatcca
     7321 tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga ccaccgtctc
     7381 cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc attccgtctc
     7441 cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac ataattaagt
     7501 cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac aaaagtctta
     7561 cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat tgtttacctt
     7621 cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa gtataatttt
     7681 ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta attgaggatg
     7741 ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa agtaggtatt
     7801 aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt ctcagatgat
     7861 attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca attattgcaa
     7921 tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga ctcaatcgta
     7981 gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat cccttgggca
     8041 aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc tgaagaaaaa
     8101 gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc agctggtgct
     8161 atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga caaaatcatc
     8221 tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt aaataatgaa
     8281 tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac acaaatgctc
     8341 ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc tgacaatact
     8401 atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga gctacaagga
     8461 aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac tgaattggtt
     8521 tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa aaaacacaca
     8581 gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga tgtcattgga
     8641 acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc tggtgatttc
     8701 caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg ccaaatgatt
     8761 cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa aagaagcctt
     8821 tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac tcgttcaatg
     8881 aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat ctgtcttgta
     8941 tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct tcgttgcacg
     9001 atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat gtataaagag
     9061 cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga aaaagtagcc
     9121 agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg gaagactaaa
     9181 catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc aatcttcaat
     9241 gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg ttgctgctcc
     9301 catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta gcactttgtt
     9361 tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat ccccttcgct
     9421 ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat gaaacatctt
     9481 tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa gagctgtttt
     9541 ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt cttttgaagt
     9601 gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat ccgtttcttt
     9661 ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag aaggcacaac
     9721 agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag ctaaagcgac
     9781 gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct cttttttcat
     9841 ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt tttataaccc
     9901 gcgaattgta aaattaagtt agaaaaataa aaaggcattt atggtacact caaattgtat
     9961 ttccgacgaa agaaaacaaa ggagtgtaac cataaatgac ctacaaacat cttaccatag
    10021 acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt gaaatcgcta
    10081 accgaatggg acgtgctata caaactattt ataatgtagt caataagttc aagcaaggca
    10141 agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt ggtagaaaag
    10201 tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact cttggttgga
    10261 cgcctgacgt cattatcggg cgaaaagaaa ggtctgtttc atgcggtatg agaacacttt
    10321 atcgtttatt ttctaaagga atatttgata ttgacacact accgatgaaa ggtaaaagaa
    10381 aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca atccatgatc
    10441 gacctgataa ttatcctgat ttcaattctg agtttggtca ccttgaaggt gatacgatcg
    10501 ttggcattca tcataaaagt gccgtcatta ctttagttga aagattatct aaagtcatta
    10561 tcacgattaa acccaacggc cgtaaggcat tagatattga aactgccctt aatcaatggt
    10621 tttctcgctt ccctaaaaac ttctttaaat ctattacgtt tgactgtgga aaagaatttt
    10681 ctaactggaa agccattagt aaccaacatg atattgatat atattttgcg gaccctggaa
    10741 caccttctca acgcccatta aacgagaatt ctaacgggat tctgcgtcgt aatggactgc
    10801 cgaaatcaat ggattttaga aaagtgaatc agacatttat ttccagtgtc agcaatcaac
    10861 gtaatcatat tccaagaaaa tcattgaatt acagaacacc aatcgaaata tttttgagct
    10921 atgtacaaga agcattttat tctagcttaa tttgacaaat cataacctat aaaaaaacaa
    10981 aataaaaaag cagaaataac tggctatttc tagcggatta tttctgcaat aactaattat
    11041 ctatttttta ttttcaatta atttaaagct tgaccatggt ttcaaaaagt caaggaggaa
    11101 taattcgtca tcggagattc ttcctacaac attgacacgt ccgtcgtttt tcatatcctt
    11161 caaggcgatt tgtgtttctc ctttgtattg accgtaccct tcattgtcaa tcagtacatc
    11221 cccatgatgc atatctcgcg tattatgagg cgggaaatct ttatctttgt agtatacacg
    11281 agtcatcgtt gaacgaagga tatattctga gcgatcccct cgataactat gaaggttatc
    11341 aaacaagcag attcgttcgt tttctgtaat accttcttcc gtatctactt tcaatgttgg
    11401 ataatccgcg ttaaatgctt ctgccatttc tttcaattct tcttcacttg cgtacgcatt
    11461 tccaatagaa atatcatcaa tcaatcctgt aaggattaaa tgcttcactt gtgtcgcaat
    11521 ttcaagatct cggtggtctt ccagcgtgca caagccatct tgcgtaggcc aagggccaaa
    11581 ctcagcagat tgcgaattga cgaatgccat cgtattcaaa ttatattttc tgaatttttc
    11641 tgaacaaaaa acaaagtgtt cgtatcctaa accagagtat cgatgaggat aaaaattatg
    11701 agaacctaat aaattatccg tgtttggcga ataactcatg atgttgtcaa cgtaattcgt
    11761 gcccgaactc atattgatct cgattttgat accaaaagga ttgcgcgtca tttttgcttc
    11821 ttctgctcct gtgaatccga tatctaagcg gaccccgtag gcacccatct tatggaaaaa
    11881 agataagtca tcgtaagaaa tctccaactg ttcaaataac gctggattga tatcaaccat
    11941 tacttccatg cctaggctat tcgcgtaatc aactacttct ttaaattctg aaagaacttt
    12001 ttctttatca tcattgatct gcagcaaact ggtaaaaacc cgtttatacc catacttatg
    12061 agctaaatcc aagtacgctt tgtccttttc aaatgttgac cgttctggat aaatcgagat
    12121 tcctaatttt cccatattac catttcctcc tgcatctttt cgtttttgta tccacttaca
    12181 ttatagcaaa cacaaagtaa atggtctaca ccttttcttt ttcttttaaa ataaatgata
    12241 aattccaata atttaagtag gtattttccc tctattttgt tatcattaaa gataacaaaa
    12301 tagtaaattg gaaaggaaca gttatgaaga aaaataaagt tattttaagt attagtttag
    12361 cagcagcatt gctgtttgct gctggttgtt ctgcaaacaa cacgacttct tctagttcgt
    12421 ctacagaaag ttcaaaaagt acatctactg cggaaagcac gactgccagt agcgtcgatt
    12481 tgaacgcatt ggaattacca caattgacga aagatgtcgc cgaaaatgaa gatctggtcg
    12541 aaatggtgac gaataaaggc acaatcaaaa tcaaattatt tcccgaacac gcaccaaaag
    12601 cggttgaaaa ctttatgaca catgcaaaag acggttacta caacggactg actttccatc
    12661 gcgtcatcaa agatttcatg atccaaggtg gcgatcctaa aggcgatggc actggagggg
    12721 aaagcatttg gggagaagga tttgaaacag agatcaacaa ccacctttat aatataagag
    12781 gtgcgttatc tatggctcgc tcacaagatc ctaacagtaa cggcagccag ttctttatcg
    12841 ttcaaaatac agatgatatg catgatggtc ttttaaaaga cgactatcct caagcaatca
    12901 tcgacgctta taaaaacggc ggttatcctt cactagatgg ccattatact gtcttcggcc
    12961 aagtgattga aggaatggat gtcgtggatg ctattgcaaa tatcgaaaca gatagttcag
    13021 acaaaccaaa agaagaagta aaaatcgata aaatcaatat actgcaagaa gcaaaacaaa
    13081 aataaaaaaa taacgtctga atcctgcata gatgacaacg tatgctagga ttcaggcgtc
    13141 ttctttttac ataaaaagca agtaagcttc cttttgtttt gctatatcag gttttatagt
    13201 caagcatttt ggttgacgat gaacgtgaac tcgcactcac agactttttt atcatctaca
    13261 tatgctgcag ataacgctgt accgactgat cccttcattt tggtgatttc aaaatgacat
    13321 ttcagcacat ctcctggaac aactttttga cggaatttgg ccttattgat cccaccgata
    13381 taagctcttt ttcctagaaa ctcttctgat tttaggataa gtatcgaacc tgcttgtgcc
    13441 agtgtttcta gaatcaacgc acccggcatc actggattgt caggaaagtg accttggaaa
    13501 aaatcttcat tgattgttac attttttgtc gcaacgatct tttttcctgg ttctaaagta
    13561 tctacataat caatataaca aataggataa cgattaggaa tcaattccat tacttcagta
    13621 gcagataata cgtaagacat aattaaactc cttctttcag gcaattgcca ttttcattac
    13681 tttgactatc aaagtatcat attcctaaat atttagcaat ctttttttaa tttaagaaaa
    13741 aatagtttga caatcaaaac attttgacta ataattaaga aatcaagaag ttattttagc
    13801 tattattctc tattttttta cgactatcaa atttttatca gaaacaaagg agagtcatta
    13861 tgtcatttct agaaggaaaa aaaatcgtca ttatgggcgt cgctaacaaa aaaagcattg
    13921 cttggggttg tgctgaagca atgttgaaac aaggggcaga aattatctat acttaccaaa
    13981 atgaacgaat gaaaaaatca ttagtcaaat tggtcggcga agaagcattc actgtagaat
    14041 gtgatgtttc tagcgacgaa agcatcgaaa aagcaatgaa tgccatcggt gaatatgctg
    14101 gggagatcca cggccttgtc catgcagttg cttacgccaa caaagaagaa ttgtccggaa
    14161 atgtgtctga tatttccaga gacggctatc aattagctca agatatcagc agctactctt
    14221 taattgctgt atcaaaatac gcacaaccgt ttcttgcacc aaacagcggg atcgttagca
    14281 tgacttatct aggttcagaa cgcgcaatcc cgaattataa catgatgggt attgcgaaag
    14341 cttctcttga agctgctatc cgttatttgg cagctgagtt ctcgcctaaa ggaattcggg
    14401 tcaatggaat ctcagctggg gcaatcaaaa cattagctgt cacaggtgtt aaagactatc
    14461 aaaaactgat ccagttatct gaatctcgta cgcctgatca gcaaggtgtg acgatcgaag
    14521 aagtcggtaa tgcttgtgct ttcttaatca gcccactttc cagcggaatc atcggtgata
    14581 ttatctacgt tgacaaaggt gtccatcttt cataaacttt tgttgcattc tttaaagtaa
    14641 aacatataaa aatcaggaaa aaacttttta aagtctcttc ctgatttttt ctttttacgt
    14701 ttttttcaac tcattacttt gaaattattc cacgacttca atccagcctt ctggtgcttc
    14761 tatgtctcca aattgaatac ctgtcaattc atcatacaac cgttttgtta ctggtcctac
    14821 ttccgtttca ctataaaata catgaagatt gttattgtga taaatcccac cgattggaga
    14881 aataacagcc gctgtaccac atgcaccagc ttctgcaaat tgatctagat catcaatgaa
    14941 aatatctcct tcaattgctt gcagtcctag tcgttcttca gctaactgca acaaggaata
    15001 tttcgtgata cttggtaaga tagagggcga ttgcggagtg atgaattttc cgtcttttgt
    15061 aattccaaag aaatttgctg cacctacttc ttcgattttc gtatgtgtgg ctggatcaag
    15121 ataaacacaa tctgagtaat ccagttcttt tgcttctgtt cctggaagta aactcgctgc
    15181 gtaatttccg ccgacttttg ctgcaccagt ccccttagga gcagcacgat cgtaatcaga
    15241 tacaataaaa ttcgttggtt ttagtccacc tttgaaatac gctcctactg gcgtacagaa
    15301 gacgacaaat aaatattctg gagctggatg gacaccaatg ttttcaccga ccccaatcag
    15361 taaagggcgc aagtataatg tagcgcctgt tccatagggc ggcacaaaat cttcatttgc
    15421 ttttaccact tgcttgacag catcaacgaa catcttttca gggaccattg gcatcagcaa
    15481 tcgtctagca ctttgattca tccgtcgact attttgttca ggtcgaaaga gattgacctt
    15541 gccgtctttt cgacgataag cttttagacc ttcaaaacat tgctgaccat aatgaagcgc
    15601 tggagagcct tcatgaatcg tcaggtaatt atcttctgtc aattctcctt cttcccattg
    15661 tccgtctttc catttggcaa taaaacgcca aggagttttg atataagaaa aacctagatt
    15721 gttccaatcg atttgcatat acttaccacc ttcgttattt tctccacatt ttatcattat
    15781 ccatattatt tgtcatttta ttttcagctt ttttccttca ttatagttat ttttgctatt
    15841 tccttttttg aaaacttctt ggtacaatac agaaagaatc aaattttaga aaagaggatt
    15901 ttatgttttc attattagca actgccggta cgacagactc ttctggcgag acattgacag
    15961 atcaagctgt cagcaaagta aatgcgtttc agcggttttg ggacgggatt gattgggacg
    16021 ccattttggc tgcctttatc caaaaaagtt tatatctgat ttttttgatc gtcctttttt
    16081 ggattcttca ccgtgtggga aaatatctga ttgataagtc ttttaatcag tattctaaaa
    16141 aagccattct aaacgagagc cgcttaaaga ctttacatag tcttttaaat actatttttc
    16201 attataccct tggtttcttt tttatctatg caattttatc tgctatcggt gtgccgatcg
    16261 gatcactttt ggctggtgcc ggaattgcag gtgtggcaat cggtcttggt gcccaagggt
    16321 tcatgagtga tgttattacc ggatttttta ttatcatgga acaacagatg gatgttgggg
    16381 actacatcaa gctagcaaac ttaacgattg aaggaaccgt cgtatctgtt ggtatccgta
    16441 cccttcagct gaaagcaacg gatggtactg tccatttcat cccgaatcga aacatcacca
    16501 cgatcagcaa tctctctcgt gcaaatatgc aagtactttt agatattcgg attgttcctg
    16561 aagaaggata tgacaaaatc tatgagatca ttgatcgagt aaatcgtaca ttagctgaaa
    16621 aataccaaga agagctccaa actgaacctt ctatctttgg attagtggat cttggaaaca
    16681 gcaattttgc gattcgtacg gtctgctacg cattgaacgg aaaacaatat gccttaaaag
    16741 aagagttctt gagctcctat gtcaaagaat tgacagcttc tggtttcacg attccgaaca
    16801 gtccaattgc tgtcggaaaa taatttggac aaacgatgca acgaatctca ctaaaaagag
    16861 cgtgggacaa aagtaaagaa cacttttgtc
//
LOCUS       DASJSZ010000060        16594 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.061, whole genome shotgun sequence.
ACCESSION   DASJSZ010000060 DASJSZ010000000
VERSION     DASJSZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.061"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(131..1342)
                     /locus_tag="U4B03_001890"
     CDS             complement(131..1342)
                     /locus_tag="U4B03_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2068735.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1461..2096)
                     /locus_tag="U4B03_001891"
     CDS             complement(1461..2096)
                     /locus_tag="U4B03_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEN2068736.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2232..2885)
                     /locus_tag="U4B03_001892"
     CDS             complement(2232..2885)
                     /locus_tag="U4B03_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068737.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(2988..3194)
                     /locus_tag="U4B03_001893"
     CDS             complement(2988..3194)
                     /locus_tag="U4B03_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEN2068738.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3270..4016)
                     /locus_tag="U4B03_001894"
     CDS             complement(3270..4016)
                     /locus_tag="U4B03_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2068739.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4176..4400
                     /locus_tag="U4B03_001895"
     CDS             4176..4400
                     /locus_tag="U4B03_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HEN2068740.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4424..5185
                     /locus_tag="U4B03_001896"
     CDS             4424..5185
                     /locus_tag="U4B03_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEN2068741.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5197..5454
                     /locus_tag="U4B03_001897"
     CDS             5197..5454
                     /locus_tag="U4B03_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068742.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5546..5797
                     /locus_tag="U4B03_001898"
     CDS             5546..5797
                     /locus_tag="U4B03_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2068743.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5773..5946
                     /locus_tag="U4B03_001899"
     CDS             5773..5946
                     /locus_tag="U4B03_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068744.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(6060..6467)
                     /locus_tag="U4B03_001900"
     CDS             complement(6060..6467)
                     /locus_tag="U4B03_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068745.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6628..6852
                     /locus_tag="U4B03_001901"
     CDS             6628..6852
                     /locus_tag="U4B03_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068746.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6862..7056
                     /locus_tag="U4B03_001902"
     CDS             6862..7056
                     /locus_tag="U4B03_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068747.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7059..8081
                     /locus_tag="U4B03_001903"
     CDS             7059..8081
                     /locus_tag="U4B03_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEN2068748.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8044..8859
                     /locus_tag="U4B03_001904"
     CDS             8044..8859
                     /locus_tag="U4B03_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HEN2068749.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8877..9659
                     /locus_tag="U4B03_001905"
     CDS             8877..9659
                     /locus_tag="U4B03_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068750.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9659..10507
                     /locus_tag="U4B03_001906"
     CDS             9659..10507
                     /locus_tag="U4B03_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2068751.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10504..10746
                     /locus_tag="U4B03_001907"
     CDS             10504..10746
                     /locus_tag="U4B03_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068752.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            10889..11257
                     /locus_tag="U4B03_001908"
     CDS             10889..11257
                     /locus_tag="U4B03_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEN2068753.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11272..11433
                     /locus_tag="U4B03_001909"
     CDS             11272..11433
                     /locus_tag="U4B03_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2068754.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11430..11756
                     /locus_tag="U4B03_001910"
     CDS             11430..11756
                     /locus_tag="U4B03_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068755.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11753..12568
                     /locus_tag="U4B03_001911"
     CDS             11753..12568
                     /locus_tag="U4B03_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEN2068756.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12707..13018
                     /locus_tag="U4B03_001912"
     CDS             12707..13018
                     /locus_tag="U4B03_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN2068757.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13042..13260
                     /locus_tag="U4B03_001913"
     CDS             13042..13260
                     /locus_tag="U4B03_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068758.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13257..13673
                     /locus_tag="U4B03_001914"
     CDS             13257..13673
                     /locus_tag="U4B03_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEN2068759.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13670..14008
                     /locus_tag="U4B03_001915"
     CDS             13670..14008
                     /locus_tag="U4B03_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068760.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14005..14235
                     /locus_tag="U4B03_001916"
     CDS             14005..14235
                     /locus_tag="U4B03_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068761.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14242..14445
                     /locus_tag="U4B03_001917"
     CDS             14242..14445
                     /locus_tag="U4B03_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEN2068762.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14442..14738
                     /locus_tag="U4B03_001918"
     CDS             14442..14738
                     /locus_tag="U4B03_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068763.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14815..15228
                     /locus_tag="U4B03_001919"
     CDS             14815..15228
                     /locus_tag="U4B03_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN2068764.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15241..15627
                     /locus_tag="U4B03_001920"
     CDS             15241..15627
                     /locus_tag="U4B03_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068765.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16178..16405
                     /locus_tag="U4B03_001921"
     CDS             16178..16405
                     /locus_tag="U4B03_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068766.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16402..>16594
                     /locus_tag="U4B03_001922"
     CDS             16402..>16594
                     /locus_tag="U4B03_001922"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068767.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5632 a         2697 c         3455 g         4810 t
ORIGIN      
        1 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
       61 ataccgcaaa ttatcccaag tttttgtata tcctacccga gtttataccc gaaaatataa
      121 ttttttagtg ttattttata tcttttttta ataggttttc aaacgctgta gttgctgtat
      181 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      241 ccaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      301 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      361 tgaacttctt atacagataa cttccacgtg gcaaagttcc atcttgtttt gtatcaaata
      421 taaaatagtg ttttaatttc tttatattta atttccaacg aatcttttta ttctctttga
      481 ttaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      541 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      601 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      661 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      721 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      781 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      841 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
      901 ttgtatttct tgataaaggt ttatcagagt ttctcccagg atcttttgta ttgattcttt
      961 gagcattaac aacaattttc tttatcatgt atggtttgat atcgattaga gggataaaat
     1021 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1081 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1141 tgaacaaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1201 ctttatatgc cgctctttta ctacttgctt ttatagtttt gctttttctt tttgccgagc
     1261 catcaggttt gtatcctaat acagctctta gtctatatgt ttcgttgtct acttgttcta
     1321 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1381 ataaagaagc ctatcgtata ggtttgtttc tttcttagaa cacgctcgct ttggtcggtg
     1441 gggcgtgttt tttatactaa ttagttgtga agagcaattg aaactgtctc tgttccaaag
     1501 taacctgttt gtgcttgcaa tacattattt tttgcatttg cttgggcttc agagatatca
     1561 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1621 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1681 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1741 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1801 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1861 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     1921 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     1981 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2041 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2101 atattcctca tttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2161 ttgcagcacg ggctttttta tttataactt ttttagagat tataggcaaa atagtagggc
     2221 ataaaaatat attattgaat tccgtattta aaaaatccta attgaacttc accggaagtc
     2281 ttttgctgtg ttgtacgcaa tgcttcttct tcagacattc cattctgtac tttccatgca
     2341 acaggcgaca tcccgtattt gttaacaaaa tcagtaagtg ataaagtgtc agcgtcttgc
     2401 tgagcgcttg tttgttggtc ttctggattt tgttgagatg ctgcttgttg ttctttctga
     2461 tcttgactga taatattgcc agcatcatct gtagtcaatc cattttcata aagggccacg
     2521 ccgaaagctt cccactcttt gttggaccaa tttgcacgat cagctggagt tgactgtaaa
     2581 gtgcgttgtt tcatctgttc atatgtttct tcttgaggtg cggtttggat tgtactctga
     2641 ctggagctta taactgttgg gctaggttcc gccgtaactt ggttggagct tgtaactgtt
     2701 gaactagaat ctgttttaga tgtagatttg ctagtagatg aactggtttc agttgtttct
     2761 tttgttttac ttatttttgt ttcttggtta gaagtggcat ctgttgattc agctttttta
     2821 ttatttgaac aagctgaaag tagcagagca gtacttaaca acaacataac gctaactttt
     2881 ttcattttat actcctcttt tctttgatac aataagttgt aaagaagcct actataggtt
     2941 taacatccca cgtccacaaa ctttggtcgg tgggcgtgtt tttttgttta tagactttcg
     3001 aaaactaaag tagcttggat tctatcgcca ccaccgaaac ctttactacc accgtttgta
     3061 gtggaaatag tgtgtaatcg ataaccttta gaacactgtc tatttatagt gttttcaagt
     3121 tctgataggt tttgcgaccc ttttcctata aacttttctt ttaatacaac ttgcaataca
     3181 acgtaacttg gcatattctt ttccttcttt ctactatgat aataaatact aacccctagc
     3241 cggagcgcca actacgactg gggtattttt tagaatacga ctttcccgat tattctaatc
     3301 tgttccggtg agacgactct atcttcatat aattcgttta tagagcgtag aataatcgtt
     3361 tgactctcat aatccttata tacttcttta cacgtcacac cgtcaccatc tatttcaacg
     3421 atagcaatct caccgtcttc cacgtctggc tgttccttaa caaatacaaa ttgtccattc
     3481 ctgataactg gttccattga atcgcctata ataggaacag caaaatcagc attgtttgga
     3541 actctttcaa attcttcttc ctctacaact gtatcgccat aaactaattc agtcgggttg
     3601 gcggcagaag ctgaattttt gattgtcgga agtttttcct ttttaggaaa gtgcaccacg
     3661 tttcctcgat tttgctcttc gagttgctgt tcagcaaagt tgtagacttt tgtttgtctt
     3721 ggtttttcta attgattgta gattgtttca atggatggaa tgttattttt cttatcttct
     3781 ttaggaacat cgtaaccaag caaccatgcc tcactcacat ttaaagtttt agccaacaaa
     3841 taaagcttgt gttggtcggg cgatgactta ccattgacat attgcgatag atgacttttt
     3901 gacattttta ttccagtttc tttttgcaaa ggtaaagaca tattcaaaat atcaacttgc
     3961 tttaaatttc tttcagacat taactgatta agtcgttgct gagtcgtttg cttcatctta
     4021 tacctccttt ctgatactta ttatatacct tattgaacat aagttcaata aaaaagttaa
     4081 attaatttga acttttatgt tgacaatgtt ttatgcagga gttatacttt ggttacaaag
     4141 ttcaaaacat ttgaactaga aaggagcgaa ctaatatgag ttttgattat tctaagttgt
     4201 cgggaagaat tgttgagaaa tatggaacgc aatacaattt ttcaatcgct ttaggtttgt
     4261 cggaaagaag tttgtcatta aagttgaata accgagtacc atggaaaagc accgaacttc
     4321 aaaaagccat tgaattgcta gatattccaa aagaagaaat tggcgaatat ttttttaatt
     4381 tagaagttca aaatgtctga acttaagaaa ggaattcgaa aaaatgaaca cacaaaaatt
     4441 tgaaaacaat ctatttgaat tagaagttaa gacggaaaat ggtgaatcgt tatttaatgc
     4501 tgaaacagta gcaagaagtt tgggtttcgt ggaaacaaaa aacggtaaag aatatgttcg
     4561 gtggagaact gtcaatggat atttaaaaaa atacctttcg caagaagttg cgaaaaacga
     4621 ctttatcagt gagccaatgg tttacaaatt agcctttaaa gcaaacaacg tacttgctga
     4681 aaaattccaa gactggttag caacggaagt cctacctcaa atccgaaaac acggcatgta
     4741 cgcaacagat gaactactta acaatccaga cttgctgatt gaagtagcaa ccaaactcaa
     4801 agaagaacgc acgttacgat tgatagctga acaacgagta aatgaattac aaccgaaggc
     4861 agactattac gaccgaatct tgaacaacaa aggattagtt acagttagta cgattgctaa
     4921 gaactacggt atgagcgctg tatcgtttaa taaattgcta catgaattag gtatccagtt
     4981 caatcaaagt ggcacatggc tgctatacag taagttccaa gacaaaggat acacgcatat
     5041 tgagccattt gattatgaag ataaaaacgg caatcggcaa gtaaaaacaa ggatgaaatg
     5101 gacgcaaaaa ggacacatct tcttatatga aacattgaag aagaataatt acttaccgat
     5161 gattgaacgt gaacaaacag cataggaggt aacactatga acgaggaaat gagccaaaca
     5221 atcaaggtac aaaaaatgat agatgatcta acacatgcaa ttgatagcca agccgataaa
     5281 atcatcaagg aattgcaagg acaaaaagtt tgggatgcaa aaatgctttt caagactatc
     5341 aaatttgaaa tgaatccaac aaaaagaaag ctcgccgatg tgttggaaga aaagttggct
     5401 tccgctatca acgagcaaga attactattt gaaaccgata cttttaacag ctgatgtttt
     5461 atagatgaca gtttcatccg aatctatatc attatagatt cgattattgc aatgtacgaa
     5521 ttataaatac aggaggaaat aaccaatgga gcaactagct ttagttaacc tctcagacct
     5581 taaagcttct ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc
     5641 ggaatattta aaaaccacaa cacgcacgct gactaaagat gccgaatctg gaaagattcc
     5701 agcagcgaaa gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa
     5761 aggaggaaaa acatgggtaa attcaacaga gcactagtgt tcagcgcacc cctaatcgtc
     5821 tatgctttag gactttgggg aagcagacaa gcattaatcg gcacgatcgt ttacatggtt
     5881 tggattttta tggggcttga tgaagctgag tacagagcga aaaagcccac cgagggggct
     5941 gactaaagtg tggttctgtt tattaggtgt ttatctcgtg gccgttttag taaataacta
     6001 catgagaaaa cgtggcgaat actggtatac atcctatgcg gttctagtat gtctaatgct
     6061 tacaattttt ctaatgattt attcaaaata ggtaatactt tgtcaatcaa ataagtttca
     6121 agagattttt gttctgcatc agtgttgccg taaccaaaac ggcttaagaa aattttcata
     6181 acctctatat cttcattttg aatatatggc aggactgcat aacctgatgc ttttacagca
     6241 gatatatttt tatcagtgtt actaccaata caaataccca cattgttgag aaatgttgag
     6301 aaagttgttt gtattgtttg ggtttttgaa tcgtgttttt gtaattcaat ttctttatcc
     6361 ttcatcgatt caacatgctt attatttata accgctgtaa tcgaagggga tataagagct
     6421 actaaagcta ggataatcga aattgtgatc gtgttatcga aactcatttt ttcaccatcc
     6481 agtttttaaa ccattatatc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
     6541 aaaaataaaa aaagacacga acgccagcaa gcatgatcgt gtccaaaaga aaaatctttt
     6601 aaaccattat aacacaggag gaaataaatg atctcagtta aagggttagg cgatgaaata
     6661 ttcgaagcaa tgatgagcaa agcacaacag gacatcaaag aaaaaatcat ttctgctgca
     6721 gcgcaaggac aaactagctg cactgttcga tcgagaggaa cgacaccaag ttttcttttg
     6781 gcgctagaag atgaaggcat tttgaatatc aaacgaagag atggcagcgt cagattgttt
     6841 tggcaatttt aggaggccgt gatgaaagat tttgattctt taggcgccag acagcaacca
     6901 ccaaatgaag ctagtcctgt tggagttgat tggcaagaaa atccgttata tccaggtgat
     6961 acttgctatc taacagaaga aggctatgta ccagtagacg caattctaga gtacgtccag
     7021 caacattatc caaagattga attaggagga atttaggaat ggcaaatgat ttaacacaaa
     7081 caacacaacg ttcattagat gagcaggtaa tcagtaatct tggacggtta caagaacaag
     7141 gtttagaaat gccaccagga tatagtccac aaaatgcgtt aaaaagtgct tttttcgaac
     7201 tcactaacaa tacaggagga aacttgttgc agatggctgc aaacaatcaa gaaatgaaaa
     7261 cctccatttc taatgcactt ttagacatgg ttattcaagg gttatctcca gcaaaaaaac
     7321 aatgctattt cattaaatac ggaaacaagg ttcagctaat gcgttcatat tttggaacaa
     7381 tggcggtcct tgatcgtgta actggagggg cagatattac gcctgtagtc gttcgacaag
     7441 gtgatgagtt tgaagttgca atggatggac caaacatggt tgtcaaaaag catgaaacaa
     7501 aattcgagaa tttagacaat gaaatcattg cagcctatgt agtcatcaag ttaacaaatg
     7561 gtaaagagac cacaacagtc atgacgaaaa agcaaatcga tcaaagttgg gctaagtcaa
     7621 aaatgaaaga ttctggaccg caaaaggaat ttccagaaga aatggctaaa cggacagtaa
     7681 tcaatcgggc agctaaaaca ttgatcaaca caagcaacga caacgattta ttagttcaag
     7741 ctgcaaaaga tacgttagaa aatgaatttg acaatgatcg gaaagatata acgccacaaa
     7801 cagaaaaagt ggctactctc gaacaaaaat tcttttcaaa caaaaagatt actgaaccaa
     7861 tacaaaaaga accagatccg attgtgattc cagatgatat ccaaaatgaa gtaactcgag
     7921 ttgctgatgt acctgcccat ccagaaatag agcaagcaca tttgatcgaa aatgaggata
     7981 tcaatccaat tcaagaagaa ttgatagata ttccagactt tggacgcgag gaaggtgtag
     8041 aagatgtctc agaatttgaa gacgacgagt accctttctg atgagaatta ttactccaat
     8101 gaagctgact ggcagtacat gtcaacatca caatataagt cctttttaaa atgtgaagcc
     8161 gcagccttag caaagttgaa aggcgattgg gcaccgacgt cggatcccaa agccttactc
     8221 gtaggaaact acgtacattc ttattttgaa tcaaaagaag tacatgaagc gttcaaagaa
     8281 gaaaataaat ccaaaatgtt ttctagtagg aagccgtatg gattgttgaa agatttccaa
     8341 attgctgaac aaatgattga acgacttaag caagaagaag cattcatgaa catttatcaa
     8401 ggtgagaaag aaacgatcgt gactggggaa ctatttggaa cgacatggaa aggcaagatt
     8461 gattgcttga atgttgagga tgagtatttt gttgatatca aaacaaccaa agacatgcat
     8521 gagcggaaat ggaacgaaaa ctacggatca agagaaacgt tcattgtcaa ttttggttac
     8581 gtacttcaaa tggcaatcta tcaagagctt cttttacagc aatacggaaa gatgttcatg
     8641 ccaatcatcg ctgcggtatc gaagcaaacg cctagtgagg cgaagttgat aacgattgat
     8701 caagataaga tgagctacga gctaatcatg ctaaaagaaa atattgaaag aattgtcaaa
     8761 gtgaaaaatg gcgaagaaag gccgatacct tgcggtacgt gcgagtattg tcgaggtaac
     8821 ctttcaatta ctggattcac tagcatggac gatctttaaa aggaggtgct ttgcgaatga
     8881 atctaggata catcaagtta taccgtaaag tgactagttc attcgtttgg accaattccg
     8941 atatgttcaa gctatggata ttgtgtttaa tgaaggctag tcatgaagat agaaaatttt
     9001 tatttaacgg tcaagaagta cgtttgacaa gcggacaatt cgtcacagga gcccatgcaa
     9061 tagcaaaaga gtacaacgaa ggagtcccga gtgacaaagc aattgcatgg cgaacgctat
     9121 ggagatggct taagaaattt gaaaatgaag aattattgac aatccaatcg aacgctagat
     9181 atagcgttat aacaataata aattggtctg actatcaatc gagtgacaag ccagtgacaa
     9241 gcgacggaca atcgagtgac aagccagtga caacaaacaa gaatgataag aatgataaga
     9301 atgataagaa taataatcct cgcaactctc gaaaaacacg agagtatgca gatgacgatc
     9361 caaataaaaa attggccatt cttttattaa aactcattcg aaagaatcgt agtctcaaag
     9421 aacccgattt gaacaaatgg gccaatacga ttcgtttgac gattgaagct gacaaacgaa
     9481 ctggaaaaga agttcaagac atgattgttt gggctactaa ccatgatttt tggtccacag
     9541 tcatttcatc gccaactagc ttaagaaaaa atttcgatac aatggtgggt caaaaaaata
     9601 aaagaaagca acaaaatatt tctaatgatg agttaccaga aacaggtgag gattggtaat
     9661 gaataaaaaa ctaagtgcaa tggctgcacc ttacggcgga ttaaaaacag cagatcataa
     9721 ttgcccgaaa tgtggtgatc cattgtatat ctggaaaaca aaaaataaag atggtactga
     9781 tcgatgcggt cctacatgta tcaataagat ttgtggttat cgagaaatgg taactaaaaa
     9841 tcaaaaagaa gctatccaaa aagcgaatga agcaatgaaa aaggacgcta tcaatcgaat
     9901 gcttaacagt tcaatgatta cagatgatgc catatggacc ttcgattttg atggatacaa
     9961 agtagttgat caggaaacag cacaaataaa agcgatggct caagaatggg ctaaaaaaat
    10021 tgtaaatggt agcacgattc atgcagtgat aaccggtaga acaggagccg gcaaaaccca
    10081 tctaggcgct gcagtgatta aagaagtcat gagggcatct aattataaaa ttgcttgttc
    10141 atttatcagt tatcgagaac ttttagaaca gttgaaattt gcaatgaatg atccagaagc
    10201 aagaaaagcc gtaacaggtt cgctgatggc tgagataaag aaatcagatt tcgtggtaat
    10261 cgacgatttg ggtgcagaac taggaagaat ggaagaaaac aaccaggcaa cgccttatga
    10321 tgttgatgtt ctcacatcgc tcacagaagc tcgtctaaac aaagctacga tattcacaac
    10381 caatttatca tcgaagcaat taaaacacgc gtacggtgag cgagtgttct ctcgtgtaat
    10441 gaatggaacg aaagggaaca tagctgtatt caaaacaacg acagacaaaa ggaggaatcc
    10501 agtttgacct ttgtagtgaa aaaaatgtgc tacttagata gccgagggcg aggagaagct
    10561 agtgtcgagt ttgctaaaca ccacacgact aaagaagagg ctgaccttgt tgcaagtgtt
    10621 tgtggcggcg aagtagtcga agttattaaa cccaaaagac gattttcaaa acctaagaca
    10681 aagccagtga aaaaagagtg ttgttgtccg aaaagtaacc aagcatggat gagaggtgca
    10741 aaatgacttg tttaaaatgc aacgatgaaa cagttatttg gtataagaca ttgcttggat
    10801 ggtcaacttg tgaaccttgt ccaatatgca atgaaaatgg acgacgttcg aaagaacgac
    10861 tcgaaagact aaagaaggag tatagcaaat ggcaacaaga agcaaatacg gaaacaaaaa
    10921 gcacgaagta gacggcatca cttttgattc tatcacggaa gcagagtatt acgatctagc
    10981 cttgtggcaa gcacaggcga atggttggaa agtgaagctc caggaaaggt ttgagctgat
    11041 gccgaagttt gaactagagg gcaagaagta tcgcaagatc gagtatattc ccgacttcac
    11101 attttacaaa agcggcaagc tggtcaaagt cgtagatgtt aaggggatgc agacaaaaga
    11161 ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa gtgccgttga tattagccaa
    11221 aaaatatcgg aatacgttca aggaagagcg tttttaacga ggtggtccat catgacaaca
    11281 gaagaagtga ttcaaatgcg aattcgaagc cttcggcgtg agattgacga tctggaacga
    11341 acaaaggcag tgatggtcaa tgaaacggct agaaaggcaa tcgatttgca catagagaat
    11401 ttaagaaggg aaatccgtag attggaggaa tgagcgtgga taaggaagcg gcttggcgaa
    11461 aattaatgtt gctgattcaa gatgagaact ggcaagaaga tgaagcagtg gttgctgaag
    11521 ttcagcgtct aggaaagatt gctaacggac gtatacgaaa aaagccagac aaaagaaaac
    11581 agcgcaaagg gaaaatcgtc gttgttttac acgaaggcaa aattttgatg caaggaacag
    11641 ctagtgagct gtctgcagaa actggatata cgcgtgggac tattcgaacg tacgcttggc
    11701 gaaatcacac cgatcgaaaa gggcacgaat ataagtattt ggaggaagaa aaatgaacga
    11761 aaacaaatta atcaaattgg gtgtagcagg agcagtaatc gtaggtattg gagttatcgg
    11821 aggatttaag ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa
    11881 cggcggtatc aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt
    11941 gatccaatat ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag
    12001 cgacggcaaa aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc
    12061 agcaaaaatg tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt
    12121 aaaatctaag cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt
    12181 cctttcagga gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt
    12241 tgaatctaaa gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga
    12301 aacacaaaaa tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct
    12361 agacgcagaa actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc
    12421 agaagcagag gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg
    12481 ttatgaagaa gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac
    12541 tgtagttacg gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt
    12601 tcttaggttt agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca
    12661 aagaagatga caacaacgat gaaatgaaat tttaggagga gaaataatgg acgaactaat
    12721 cacaaaagta gagcagtggg ctaaagacaa gggactggat caagcagatc caaaagcaca
    12781 gtttttgaaa gtagctgagg aattcgggga aatagcttcg gcgatggcaa gaagtaatga
    12841 tgaactattt aaagatagcg taggagacgt tatcgtcacg ctgattatcc tttccatgca
    12901 aaaagggaca aacgtacaag agtgtttaga aatggcatac aacgaaatca aaggacgcac
    12961 agggaaaatg gtagatggtg tattcgtgaa gtcgagtgat ttggagggca gcaaatgata
    13021 aaaaagctca ttcaattcag catggattta tatgatatcg aatcaggagc aacactatct
    13081 gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat tttgtgggta
    13141 gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa agcggttgag
    13201 tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga cagcgaatga
    13261 taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc
    13321 attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga
    13381 aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa gataagaatg
    13441 gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc ttcgattact
    13501 tagataataa agtttgtatt gtcaaaaatt caatagatta ttccggatta gtttgtgcca
    13561 ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt
    13621 atgaagtcat cggaaatata tacgagaata gcgagttatt ggaggtagag tgattatctt
    13681 agctactgac tacatcgatt ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa
    13741 ttgtggaatt aaaagcaaga atagaatcat tttgttacgg atatcggctg atttagcaaa
    13801 atatctttat ctgagcaaga cagcagtata ttacactgcg gctacacgtt atcagtacaa
    13861 gggaaaaaca atcagtcagg attattatga tcgtttaatc agtcctgata tgtatgggaa
    13921 ttcagaatca gcaattaagc tgtttggcgc aatagagata tatgccgatg attttccaaa
    13981 tttatggttg aaggaggaag cggaatgaaa tacgaaatac cactaagtga agcagacgtc
    14041 cagtcaatta ttaacggtcg ggaggttaat aaaaaacttc ctgatggtac tgaattagtc
    14101 atcagacaaa gttatttgaa agatatggca gctccagtat taattgatcg ttttaacgtg
    14161 actgattctg tggtagagaa ccacttgaaa gaatttcgat caagtataaa cgacactttc
    14221 agattaggga gttgattgac aatgaacacc agacaccgca gagtagcaaa actaagaaaa
    14281 caggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc agcagaagaa
    14341 acatataaag tggcaagtca gtgtgttgct gatgcaagtg atgctattcg taagtttgga
    14401 atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta aaagacggat
    14461 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaatccta
    14521 aaacacgtaa gaaaccaaaa gtcaaagtcg gtgacatggt ccgctgtgaa gcagaggagt
    14581 tcatttatcc atttcgtgga tatgtagaac acgtctataa tcactcagcg atcattcgca
    14641 ttgaaaacac gatggaatgt gataaatgga cagctaaaag caaagagaat ttagcagtag
    14701 tgagattggt ggatatggaa ctaatcaatg acaaataaaa aagccggatc gctccgacta
    14761 acataataaa acagacaagt ttattatatc acataaaagg agcagtttga attgatgcaa
    14821 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
    14881 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt
    14941 acggatatgc cgaaggcacc gaagcacggc aataaggcag aggatgcatt gattcagatg
    15001 atggatatag aagcggagag agacgcgatt ttagcggctt tgatggctct tagtctgatt
    15061 agtcgtcaga tactctacta cagtttttgt gtgccagaca gcttctcaaa ctacagaatt
    15121 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaaatcgga agctctaata
    15181 gagtttgcag aagcatataa acacggaaga ataattgctt ataaataggg ggaccatata
    15241 atgtggaaag attatgtatc gttgaaagag ttgaaaaaag atcttgtttt caaaagaatt
    15301 gtagaatggt cagagagtga attaatttta gaagatggaa ccaaaatgga agttgtatgt
    15361 agcgaatcag attgctgcgc atgggccgaa ggtgaattca aaaatgtaaa gcttgatgca
    15421 gtgattacag atataaagat ttttgataaa ggtaaccgtc tctataatgg tgacggacat
    15481 acctcttatg ctgaagtcgt tgtttatcat aatcgtaatg agatcgctaa agcagaatgc
    15541 acagcgaatg atgggaatgg cggctactat tatagtgttt gtgctttaaa ggtcaaagac
    15601 aaactttgta tagtgactga cgcataaaaa aatggcggtt ttttggcgga atgatggcgg
    15661 tttttagcca tttatcagtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
    15721 caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc ttcgatagac
    15781 agcaacggaa atattaagaa taaggatgtg aattttaact ccttctaaat tgttcttatt
    15841 atctatcatc cgttgctgtt tattgttaca tacagatcgc ttaggcggtc tttttatttt
    15901 ggccgaaaac ctacattttc gatggccgat tattggagga ggaatagcat gttcaaacta
    15961 tctgaaatca ttaagaagtc cgacgttgag aaattagaga tgttaaaaaa gaagttgaag
    16021 aaatagcagt ctcttcgtga ggctgtttta ttttgctcac aaaaatagac cactatcggg
    16081 taatagtggt caggaattaa atgaaaaaga tgttagggtt gttagctaag tataacatca
    16141 taacgcttac aaaacaatgc aagaaggata aattattatg agaacttact ggtatgtgtc
    16201 attaaacaat aaatatccga agccaatgaa aggacagcat aggcgtgtag tcatgtctgt
    16261 tcaaatgaag gcgaagtatt cgattgtaga aatgctaaga gaagctacac caaaagagat
    16321 tgatcagtat aagttggttc ttattggcat tggttggttt aaggatgaac atattcaaac
    16381 aaacatgaag aggtggttga gatgaagtta tattatgttg aaaaagcaaa ttgttgggtg
    16441 gttgaaggct gtctgtcaaa gtacgctaca gatattatat ctactcaatt ttcagagttg
    16501 ggattgaagg ttatcttttg tggtgagttt gtcagtgtga aagaagcggt attgccaatg
    16561 acagcaaaga gcagacgctc gcgtggttca agtt
//
LOCUS       DASJSZ010000061        15928 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.062, whole genome shotgun sequence.
ACCESSION   DASJSZ010000061 DASJSZ010000000
VERSION     DASJSZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.062"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            111..779
                     /locus_tag="U4B03_001923"
     CDS             111..779
                     /locus_tag="U4B03_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEN2068768.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(979..1209)
                     /locus_tag="U4B03_001924"
     CDS             complement(979..1209)
                     /locus_tag="U4B03_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068769.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1336..2718)
                     /locus_tag="U4B03_001925"
     CDS             complement(1336..2718)
                     /locus_tag="U4B03_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN2068770.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2874..4061)
                     /locus_tag="U4B03_001926"
     CDS             complement(2874..4061)
                     /locus_tag="U4B03_001926"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEN2068771.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4068..5099)
                     /locus_tag="U4B03_001927"
     CDS             complement(4068..5099)
                     /locus_tag="U4B03_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2068772.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5145..6569)
                     /locus_tag="U4B03_001928"
     CDS             complement(5145..6569)
                     /locus_tag="U4B03_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2068773.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6744..7649
                     /locus_tag="U4B03_001929"
     CDS             6744..7649
                     /locus_tag="U4B03_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN2068774.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7740..8417
                     /locus_tag="U4B03_001930"
     CDS             7740..8417
                     /locus_tag="U4B03_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN2068775.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8414..8584
                     /locus_tag="U4B03_001931"
     CDS             8414..8584
                     /locus_tag="U4B03_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068776.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8698..9594
                     /locus_tag="U4B03_001932"
     CDS             8698..9594
                     /locus_tag="U4B03_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2068777.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9649..11019)
                     /locus_tag="U4B03_001933"
     CDS             complement(9649..11019)
                     /locus_tag="U4B03_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2068778.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11257..12003
                     /locus_tag="U4B03_001934"
     CDS             11257..12003
                     /locus_tag="U4B03_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2068779.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12000..12839
                     /locus_tag="U4B03_001935"
     CDS             12000..12839
                     /locus_tag="U4B03_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2068780.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12840..13789
                     /locus_tag="U4B03_001936"
                     /pseudo
     CDS             12840..13789
                     /locus_tag="U4B03_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            13930..14682
                     /locus_tag="U4B03_001937"
     CDS             13930..14682
                     /locus_tag="U4B03_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068781.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14701..15852
                     /gene="queG"
                     /locus_tag="U4B03_001938"
     CDS             14701..15852
                     /gene="queG"
                     /locus_tag="U4B03_001938"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEN2068782.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5161 a         2980 c         3208 g         4579 t
ORIGIN      
        1 tgtcacagtc ctttttttag taaaaggata catttcttcc ttgtatattc catgcgtgtt
       61 cgtgagaaag catatataat ttaattaaca gatagttagg gggagaaaaa atgatttttt
      121 caattacaat gaaactggtg ttcgggttga tcggtttatt attagtggta cgtttgcttg
      181 ggaagaaatc catgtcagaa atcacaccgt ttgatttagt ctatacgctt gttttaggtg
      241 ggatcttaga agaatcaacc tatgatgaca atgtccatgt agggcatgtc ctatttgcca
      301 ttgcgctttg ggcagtaatg atttatggta tcgaacgtat cgtccaaaaa aatgaaaagg
      361 tcaatcgttg ggtcaaaggc gagccatctg ttctaatcaa agacggcgtc atcaacatga
      421 cagagttgac aaataaccat atcgagatgg agcagttgcg agcaatctta agacagcaag
      481 aatgcttttt tcttgaaaat gccaaacatg ttatcctaga gaatgccggt caaatgagtg
      541 tattgaaaaa atcagatgaa gacaaagctt tatcgatcct gcttgtagat gaagggcaaa
      601 ttcagcataa agtgcttcag tccaatcaat tgactgaagc atggcttatg gagaacttga
      661 agaaagaagg ctatgcagat gtaaaacagc tgatttatgt tgaatggtca gaagaaaaag
      721 ggctctacat actaaataaa gaagacgtca tgcatgaatc ttaccgaata gatggataga
      781 tagacagagg ttgtgaaatc agcgccttgg cttgttactc aaaactaagc gactttttca
      841 caacctctga tatctaatat acgttgttcc agaagttgct tcttcgaaaa ataagccaaa
      901 attccacaaa aatttaagaa gcaattttcg aaaaatcctt tcttattttt tgaagttgat
      961 cacttctgtc acaacctctc atgtcacaaa ccattttttt gtttccttta actcaatata
     1021 ccgtacgtct tcccacaata tcggctgatg gtcaatcata aagtaatctt cataggcttc
     1081 accaatgaag tgcccttcga cctcatccaa gagtcttcca aattcatctt ttaaattgag
     1141 ctgaatgatt actgctttac gagataaaaa ggcttctgtc agtatactat cgatttcttg
     1201 caatgtcatt tgttgtttag gaagaggctg tttgcgtgct tcttcttcat ttttttgata
     1261 ccgttgacta gttcttccat cgcaaaagca gtcccccatt ttaatccaaa tggccgatct
     1321 cgatactcgt tatagtcaat gaattttttt gctgtccgtc tcatcttttc caccttctaa
     1381 tccactcata ccacctgcat gcccgccaac gagagaacta cgtgcgatgg cgcgtcctcc
     1441 ttgcaagaga ctatgcgcat ggatgattga ttgaaagccg tatttttttc taatcgtatc
     1501 gacaacaaaa tcaactttcg cttcatttat ctgcttttca ggatcttcaa aaaggttaag
     1561 ctgtaaacct gtcgcgtata cgaggtcgga acagttcacg cctacacgcc gaatatcctg
     1621 gtagtggtaa tgctggtgga aaatggtcaa taaagcatct gcaagatctc tgctagcatt
     1681 tgtcgcttcg attttcattt gctggtgaaa cccggtcttg ccatttggat caagatagcc
     1741 taaagagtat cctacccata agctgactac ttgtgttttt gctgcagatc gtctcaagcg
     1801 agtgccgact tggtcggcca tttctttgat aacgatctcg atttcttctc gtcttgtata
     1861 atctctatct aggatttgac tatttccgat actttttgat tttggtgtat attcttgtcc
     1921 caaaaaactt cgatcgatgc cccaagcatg ggcataaagc tgagtaccga tgacccctag
     1981 cttgcttttc aagtgataat aatccgcgtg agctaaatca taaacagaat gtatacccat
     2041 ttgtttcaga cgttttgctg ttcgtttgcc gattccccaa aagtcagtga tatctgagat
     2101 tttccataac ttttctggta catcttcata acgccattca gcaaacattt gctttccgtc
     2161 ttttgagcca ttgtctaagg ctagtttagc aagtagcggg ttatcgccaa ttcctatagt
     2221 cgtgtagatt ccggtctgct caaacacatc tttatggatc agtttagcca tgtcgtacgc
     2281 atttgtttta ccaaagagtg ccatgctatt tgttacatca aggaagcttt catcgacgga
     2341 atagacatga tggttcgctt cgtctgcata ttttttatag atagaattga tttctctgtt
     2401 cttttccata taataggcca tccttggggc aacaatatat aaatcacttg gataggggaa
     2461 gggaagatct ctggcacggc tgatattggt gatgccgtaa gctttttttg ctgcaggact
     2521 ggaagccaag attaatccgc taccccgtgt ttctgtcgaa tcggacggat aggacatgac
     2581 tactaattta gttgtaaggg gatcaagatc cttttcaacg cactctacgg aggcgtaaaa
     2641 agatttgcag tcaatacata aaatatctct tgaaggttct ttttcataaa caaataccgg
     2701 attttgattc gcccacataa caatcacctc ttcaacacaa ttagaacaca cgttctcttt
     2761 ccgtgtcaat aaaaaacagc aattttcaaa tacgagcaaa aggctcacat ttggaaattg
     2821 ctgagaggtt atttttatct atttgttttt agctgagatc gttgattttt ttatcaaaca
     2881 tgaacagctt ctggatcgat ctttgttgtc ccatttccaa tgtgtgcaac ataaaattcc
     2941 ggagcatagc cgacaacttt catgtattca tcatagacat tgtttttgaa ggcaggtata
     3001 ttttcttctt tgacaagagc aatcgcacag ccaccaaatc cagctcctgt cattcgtgca
     3061 cctaagacac cttcttgttt ttgagcagca tctactagtg tatctaattc gatcccagtt
     3121 acttcataat catagcgaag agagtggtga gaatcattta acagttgacc aaatgcttct
     3181 aagttcccag cttctaattc agcttttgcc ttcaaagtcc gctgattttc agaaacggca
     3241 tggcgagctc tgcgtatcaa tgtatcatcg ccgatttggt caatagttga ttcaaattct
     3301 tcttcatcta agtctcctaa agaagagata ggcaaagttt tttgcaaacg tgccaaggct
     3361 tcttcgcatt cgctacggcg ttcattgtat tttgaatctg ccaattcccg ccgcttattc
     3421 gtattcatga tgacgattgc ataaccatct agtttcactg gaaccatttc gtatttcagc
     3481 gtatttgtat ctaataagat tgcatgatct agctcaccga agccgatggc aaactgatcc
     3541 atgatacctg aattgacacc gatgaattca ttttctactt ttttacctgt ttggactagt
     3601 tccaagcgat ctgtttttaa atcaaacaga tcttctaata caacacctac taataattct
     3661 aatgaagcgc tactggaaag tcctgcacca ttagggattg ttccttccac taataactca
     3721 aacccatgat cgattgagtg tccagcttct tttaatttaa ggaccattcc ttttacgtaa
     3781 tttgcccagt ttgctttttt atcatattct agctcatcta aagtaaaact aatgacaccg
     3841 atatcgtcaa agtttgtgga ataaactaat attttattat cgtctcgagg tgctgctaca
     3901 ccttgcgttc cataagtgat ggaagcgggg aagacatggc ctccattgta atctgtgtgt
     3961 tcaccaatca aattgatgcg tcctggtgag aagtattgtg tagttttttt aggtccgaat
     4021 gtttcggcaa attttgtgtt taagcttctt ttgatttcca tgatgagtta ttcctttctt
     4081 gttttgattt tgaattctgt tactgtttca aatactgatc cggcttttaa atgaatcgaa
     4141 ccaaatgctg agaagcgctc tgcacctgga gctgtttgag tctcaaatgt gatgccaccg
     4201 tgatgtgcca gttttcggtt gcgcatttct ggtgtttctg tgccgaaatt tgcagtgaaa
     4261 ataaccacac tagaagcatc tgtagcaata tctacttgga ttcttttatc ggggctagtc
     4321 aagcgagcgg cttctttacc caagccggtt tcttttaaga aaaatggatg atcgattccg
     4381 tcaacaagat ttttttgatc caaatcacta gcaaaaacgg acgctaattt tttaggtatt
     4441 tgaaaatcga aagcacttcc tgcagcattt tcttttaccc ctataggaat actatctgtt
     4501 cgcaaaggag cataagcttc gctattgagc caaagctcat gttgatcgat tgactgagaa
     4561 gcatctcccg tcaagttgaa gtaaacatga tttgtcggat tgaataaagt atcttggtca
     4621 ctggtgccgc gagaggtcac tcgccagata ttatcttttg tcaaagtata gcggatttcc
     4681 acatccattg tacctggaaa accatgttct ccgtcgggac ttgtagtagt aaaaatgaca
     4741 gaagcttcat tttcgccatt caaaatgaca taagaccatt tttttaattc aaatccagga
     4801 gcaccaccat gcagattatg tccagttttt ggatctgtgg ctaattggta agtttttcca
     4861 ttgagagaaa agcggccatt ttcgattctc cctgcggtgc gaccgataga agcaccgata
     4921 tacggatctt tttcgatata ttcttcagcg gtgtcaaatc ccaaaactaa ttctcgatca
     4981 tttgatttta aactgacgat tctagcacct aaatcagaga tagaaagtgc tagcttgttt
     5041 ttgttctcca aggttatcaa gtgataacct tggccaaaat ctttttctgt aatcttcatt
     5101 ttcaagatcc tttcttaatg ggaaggaact actgcatcgt gaagttaatc ttccattgta
     5161 tcagctggag ccaatttgtt ttccaatttc tcaacgattt gtgcatgttt tttctctgtc
     5221 aatgtaatct tccaagcaaa aataatagcg gagaaaatca tcaaagcagc aggagcatag
     5281 aatgcataag ctttgaaagt cgttacgcca tgagcagtga tatcagcagc agtagcatta
     5341 ccagtcatcc cagaagcgat agcgacaaac cctacgatcc cgttagacaa agcaccagcg
     5401 attttatcta gtaaagggcg aattgataga gtaacagctt cgttacggat accatttttc
     5461 cattgaccat attcaacaga gtctgtgatc gtcatcaaag cggataagaa aatcaattgt
     5521 tgagggaagt agaacaatac tagcccgatg ataacgattg gcaaacttga acctgcgatc
     5581 gtaaagaaaa agtaagaaga aatcatcatt gcgatccctc caaggtaaac gtagcgacga
     5641 gagatgacgc gtgtaagcat tgggaatagt ggaacagcta ctaaaccaag taaggcagca
     5701 actagaccaa cgatccaaaa ttcagttggt tggccaagta catatttgaa gaagtagaat
     5761 aatacggcag ttgttgctgc attggcaatc gcataagcga tataagcaag ggcaagccac
     5821 atcaactgat cattttgagc aactgctttg aacacatctt tgatcccagc tttttcattt
     5881 tgctgacgga tcgcagtatc gacttctttt gtgcccaagg cagtgataat cgcagtaccg
     5941 cctgaaataa cagcagcaat cacaccgaac cagaaccagc ctgaagcgcc ttcaatatgt
     6001 ttatgagtaa caagataggt aaagaaagta acgataggga caacgactag agtagtacca
     6061 tttgcaccta gagaagagcc gaaacgagca aacgtagcaa atttttcacg ttcctttgtt
     6121 tccgtactca aagctggaat cattgaccaa aaagcgatat ccttaaatga ataaaaacta
     6181 tccaataaga tgaaaacgat tgcaaataat atgataaata aagtcgtatt atgagtagct
     6241 aagccaaaga aattggtaaa gattgctacc aagagtacgg aactaacaac gccgccgata
     6301 actaaccatg gtttgaattt accgtatttt gtacgagtat tatcgatgat gccaccaatc
     6361 aaaggatcaa aaataatttc aactaagcgg atacctacaa ctaagcttgt aactgtagca
     6421 atcatttttg cattcgttgc cttatctgtt cctgcgaaca tctggcttgt cacgaatacc
     6481 ataaaataag tgcttaatgt aacatagaat gtgtcgtgtc caaaagcgcc taaagcgtag
     6541 gacacacgtt ggatcatttt gcttttcata atctgtattc ctctcctgtt caatatataa
     6601 tactaagtaa accggttaca acgaataaca aagatctttt aatgacttaa acttaacaag
     6661 aaatcgcttc ctttttaata tatttctgtg acttttattt aactttttgt tgcattatac
     6721 taaaggagta aggagggata gacgtggagc aatcgatttt tttaaaaaag gaacatcaac
     6781 aagtagaaaa agaattgtat tttgattttt gtggtttctc taaaacgatg ccgtttcatt
     6841 cttttggtcc tgctattcgc aatgattaca tcctgcatgt cgtgatggaa ggcaaaggat
     6901 cttatcatgt gaaaaatcaa caattccaat tgaaaaaagg tgatctgttc ttgattcgtc
     6961 caggagactc gacgttttac ttatcagatg gagaagatcc gtggatgtat tgctggcttt
     7021 catttggtgg gagtgctgca gaagaaatca ttcggcagtc gcttttcaga gaagaccaat
     7081 acacgatggt ctcttctgac atacaaaaat atatcgacat tattttagaa tgcatgaaag
     7141 atacggaaga aaccttggaa aatgaattac agttaacagc attaacctac cgttttttat
     7201 cagtcctttt aaaagatggc ggaaaggtca gcggtggtgt acaaaaggtt cattcctctt
     7261 tagctgttga aacagtcaaa tacatcgagg cacattatgc tgaaaaattg tctgttgagg
     7321 aaatcgccca gcatttagcg gtcaatcgga gccatttgtc gcgagtattc aaaaatcata
     7381 tgggtaccag tatcaaggaa tatttgatcg gggtacggat caatcgaggg gcgtttcttc
     7441 tttctctgac ggatgaatcc gtggaaagta ttgcctatca agtagggttc aatagtctag
     7501 tcgtatttag ccgaatgttc aaaaagagta ccggagaaac ggctacgaac tatcgtaaac
     7561 gtatgaaaaa cgaaaatttt aagaatattg ctttggatac cttgaaaaaa cagttggaaa
     7621 aacaaaccgc agtttcttgg acaacctgat tagtctggtt aaaatgacaa gcatcggcaa
     7681 aagaaacttg tggcggttat gctacactgt aaagcgaaga ctaaaaagga ggaaaaggga
     7741 tgcgtacact tatcatcatt agccatccaa cgatcgaaga atctttaaat cagcaatttt
     7801 tcaaagaagc ttctgctggt ttaccagcta cttggcatca tttggaatca agttatccag
     7861 acggaaaaat agatatagag actgaactac agctcttggc ccagcatgat cggatcattt
     7921 tccagtttcc gttttactgg tatagttctc ctgctcattt aaaaatttgg caagataatg
     7981 tcttggaacg agcagcacat gtattaaaag ggaaagaact aggaatcgta ttgacaacag
     8041 gtgtggcgga aaaagaatac caagctggag gaaaagaaga atatacgatt tccgaatttt
     8101 tgaggccgta tcagcgaatc gcaaataaat tccatatgac ctatcttccg ccattcgttt
     8161 tagcacaatt tatgtattta tcccaggaaa aacgttggga gaagctaatt gcttatcagc
     8221 agtatctctc attggaaggc aaaccatcgc tgactcagcg gattgactgg tttattcaaa
     8281 gagtacagga aaatcagaaa atgcaggaag aagaatctga aaaacaagca tatatcgtag
     8341 aagcactgac tgaggcaaaa gagcagatcg aagacttgac ctttacactg caagaaatga
     8401 aaggaacttc attatgacaa atgattggct gatccaacaa ttagatgaat taagcgaaaa
     8461 atcagaatat caagaccgtg cgctcttata tgaaataaag aaactgatgg ctgagataga
     8521 cgaacgaaca atgcagctgc aaggagaaat cgacggacaa tcttggaatc atctcaactg
     8581 gtgaaatttt cttataaata acaaagataa aaatgtgcac agcgacaatt atttgctaca
     8641 ataagaatat gaagcgtttg ataacgctat tatacataaa aattggtggt gaaaaaaatg
     8701 tatgatgaaa atgagtgggg agaaaatgtt cggatgaccc gaagaagaca gcaaaaacag
     8761 gcagctcgta agcggatgat catgattggg gcagcagttg ccgtgttttt gatcatagca
     8821 ggaggagttg cgggtctata ttttcatgga aaaaattcgt cagaagcgaa aagtacgacc
     8881 ataacggaaa caacgacaaa gaaaacagag acgaaacaaa caagtgagtc atcaactaaa
     8941 acaaaagaga cacaaacttc tgaagaaaaa acagcaaatg gtttgccaga gcaacttcaa
     9001 gcaatcgtaa atgattattc gaaaaagatc gaagaaaaga cgcctgcatt gattgaagaa
     9061 taccagacag aaattcaggg aaatcaagag ggaatagctg gtttatcagc agttgctaac
     9121 caaaaagcac gtgagcttca agcaatctcc gacgaaggga tcagaaaatt acgtgcagcg
     9181 tatcaggcag cagaaaataa agacggtgta gacttagata ctttgatcaa tcaattatcg
     9241 gctaattata cgaatcatgt cgctaagatt tccgatattt atttgcagac tagcgcctct
     9301 cttcaagcag agtcaacaag cacacaagac acaacatctt ctgattctga gacagcagag
     9361 tcggtagaag cttctgaaat gcaagctcga acaacacaag acacaacaga ttcctctgaa
     9421 actaatacca acagcgcgac ttcacaggct gcgacaaccg tagttttgga aggagaaggt
     9481 ccaaatcaaa tagccgaacg aactggcgta ccagtagaaa cgatcttgtc gctgaatggg
     9541 atgagcatgg agaattattt cctgactccc ggtgagacgc taaaattgaa ttgataaaag
     9601 caataaaaaa gaacgaagct tccactaagg tgaagcttcg ttcttttttt attttccggt
     9661 tcttttttcg cgtacaaagt aatggatcgt catgtaaagc aagaaaacga gtccaataat
     9721 caatgagatt ctcgtttcag gattgataaa catgaaaatc aatacgatag ctaaaaaagc
     9781 aagtgtcaca taattcgtat aaggagatag tggcatttta aaaggatgat catgcatttt
     9841 atctgcgtgc ttctttttga agcgaatctc gctgatcaaa atcactaccc aaggaaccat
     9901 accaggcaaa atacttgagc tgtatacgaa aacgaaaata ttttctgact ggttgatgaa
     9961 cattggtaga agataattta agatcagacc aattaagatt ccacctgaga ttgctacgac
    10021 aggataaaaa ggcgcaccgt gacgagatag tttcagaaat tttttagaca tctttccatt
    10081 tatggcaagt gtatagacca tacgacttgc gctgaaaatc cctgaattgc aacctgataa
    10141 tgccgctgta atcacgacaa aattaatcag tcctgcagca aatgtaatgc cgactttaga
    10201 aaaagtttgt acaaaaggtg aaccaatctc actcaattga ttccacggat agatactcac
    10261 aatgataaaa atagcaccaa tataaaaaat caagattcga ccgatagtgg actggacagc
    10321 ttcaatgatc gtctttttag gattctctgc ttcaccagca gtcattccga tcagttcgat
    10381 tccttgatag gaagcaacaa cgatcgataa tgcaaagaaa aatcctttga cgcctccagt
    10441 gaagaatcca ccatttttcc aaagatttga aatccctact gcctgaccat gattaccaaa
    10501 tccaaataag attaccccaa gacctgcaat gatcatcaaa atgatcgtta tgactttgat
    10561 gatcgaaaac caaaattcta attcgccaaa cgccttgaca gaaataagat ttgccagcat
    10621 aagaaacaag acgacaatga ttccgggaat aattgccggc aaatcaggcc accaataatt
    10681 catataagat cctacagcaa tcacttcact tattcctacg acaatatatt gaaaaatatt
    10741 actccaagcg gtcaagtagc cagccacagg atggatgtac tcgctcgcgt aatttgcaaa
    10801 agaaccagtc gaaggatcaa cgtaaagcat ttcgcctaaa gcacgcataa tcaaatacaa
    10861 aaagaatcct gcaatcgcat aggctcccat cactgagggc cctgtccaac gtatggttga
    10921 ggaggcaccc ataaacagac cgacaccgat cgttccacct agtgcgatca tttggatatg
    10981 acgggcacta agacttcgag ttaacttttc ttcttccata caaaaactcc tttcgttgtt
    11041 gagtcaacaa gaaaaaataa tcaaacgttt caacacatgt ttttcattat taggttagcc
    11101 ttattgaact cttgatgtga ttctacagaa aattttcata aaatgcgagg aaaattcgaa
    11161 aaaaactttt tcatcattct acttgaaaat gctttccatt ttagttaaac tatatttaaa
    11221 taaaaagtta ggtaaaccta aaaaggagtg taagatttgg aacgtacggc cattgtaatt
    11281 caagaccttt cagccgctta tcaagggaaa actgttttga aaactatcaa cttgacgata
    11341 aatccacaaa aaattaccgg tattatcgga ccaaacggtg caggaaaatc aacatttatg
    11401 aaatctttgc tagagttgat accagctact actggtaaag tcaccttttc tggacagtcg
    11461 gtaaactcag tccgtaaaaa aatagcctac gtggaacagc gaagtgaatt ggatctttcc
    11521 tttccaattg atgtattagg cgttgtactt ttaggaacat atccatcttt acgaattgga
    11581 caaagacctg ggaaaaaaga aaaagaacgt gcaagacaag ctttgaaaaa agtagggatg
    11641 gaagaatatg ccaaaagaca gatcagcgaa ctatcgggtg gacagcttca gagagttttt
    11701 attgcaagag ctctagccca aggagcagaa tggatctttt tagatgaacc attcgtaggg
    11761 attgatgcgt taagtgaacg aaagatcttt gacatcttgc aggaattgaa gaattcagga
    11821 aaaacgattt tgatcgtcca tcatgatctt cataaagtag aagaatattt cgatgaggtt
    11881 attcttttaa ataaacagct gatcgcttcc ggtccagtac aagaagtctt tacatcgaag
    11941 aaccttcaat tggcctatgg tgagatcatc agacatttag taaaaggagg agagaaaaaa
    12001 tgatccaatc ctttattgat ggattaatga attatcaatt cctgcaatac gctttggtca
    12061 cttccatgct tgtaggactt gcttctggag tcatcggttc ttttattatt ttacgaggga
    12121 tgtctttgat gggagacgct atctctcacg cagttcttcc aggtgtagcc atttcttata
    12181 tgtttggttt tagttacatc tttggagcca ctgcttttgg gatgctaaca gcagcagtga
    12241 ttggtttcgt gactcagcgg agccgattga aaaatgatac agcgattgga attgtcttta
    12301 gctccttttt tgcattaggg attatcttga tttcatatgc ccagagtgcg acagatttgt
    12361 atcatatttt atttgggaat gtcttagctg ttagggaaag cgatttgttg ctgacagcac
    12421 tcgttagtgg aatcgtcttg atatttgtct ttttctttta taaagaattg aagatcacat
    12481 cattcgatcc gacaatggca aaagcttatg ggctgaacac atcgctgatc cattatcttt
    12541 tgatgttctt tttgacatta gtggctgtag tcagtttaca gacagtagga acaatcttgg
    12601 tgattgccat gttgatcaca ccagccgcca cggcttactt gctaacgaac catttactga
    12661 aaatgatcat tacagctgca ggaatcggta tgctaagtgc agttgtcggt gtgtttttca
    12721 gttatagtta caattggcca tcaggagcta cgatcgtgtt agcatgtacc gcatttttta
    12781 tccttgcttt tttattttct ccaacaaaag gaattttatt tcagaaagga agcagataga
    12841 tgaaaacaaa aaaatcactc tttttaatct tggcagttag tttcttggta ttagccggtt
    12901 gcggaaagca ggcttctgac caagcggatg aaggaagtaa agaaaagttg tcagtcgtgg
    12961 ctaccaattc gatcttggcg gacatggcaa aagaggtcgg aacagatcaa atagatatcc
    13021 acagtatcgt accagtcgga acagatccgc atgaatatga agtactacca gaagacatca
    13081 aaaaggcaag cgatgcagat gttattttat acaacggttt gaatcttgaa acaggtaaca
    13141 gctggttcga taacttgatg gaaacggcta aaaaagaaga aggaaaagat tattttgcag
    13201 ttagcaaaaa tgtagaacct ctttatttaa ctagcggtga agaacataca aaagcagatc
    13261 cccatgcttg gctagaccta tctaacggaa taaaatatgt ggaggaaatc gcacgtatat
    13321 tctctgaaaa agatgcagaa aatgcgacac tctataaaaa aatgcagaag catatgtgga
    13381 aaaactaaaa gaattagata cacaagcgaa ggaaagtttt gcttctatcg aagagaacaa
    13441 aaaattatta gtaacaagtg aaggggcatt caagtatttt tcacgagcat atgatttgcc
    13501 agcagcttat atatgggaga tcaatacaga aagtcaaggt acgcctgatc aaatgaaagc
    13561 gatcattgat cagataagag caaaagaagt accggtttta ttcgtggaaa ccagtgtaga
    13621 ctcaagaagc atggaacggg tagcaaaaga aacgggtttg aaaatctatg ataaactgtt
    13681 cactgattcc atagcaaaag aaggagaaca aggagattct tactatcaga tgatgaaatg
    13741 gaatattgaa acgatccatg aaggtttgtc acaaacaaaa gaaagctgaa aagtgcaagg
    13801 tctagcttga ggagcagacc ttgcattttt tataagagat atgaaaaaaa attatgctgt
    13861 ttttcgtcgt acagatacct tttttgaaga aattccgtta tagttagagt aaggaaggaa
    13921 ggtgtggcta tgaattttac acgtaaagat aaatatacga ttaaagaaga agtctttcga
    13981 ttgatcagcc agcaggaaaa taccgacaag gatcaagacc ttcaatttct tgaagcagaa
    14041 ttagctaaat tattcatagc aaatcaaaat gagacaacag aaccagatga agagctgtac
    14101 ataggcaaat ggttgtttga tgaatttcag cctttagatg aaaataccgg tatcggttat
    14161 tttgtgttcc tttctccagt cggcttcaca cctggcgtat tagttaaaca aaacgacgaa
    14221 ataaccgtca aagcatggcc tgcattattt tcagtcatca aagcagaagg agtaatcaaa
    14281 aagatccagt cggcctatta taaaagtcaa tttattgcca acagcaaatt aatgaatatg
    14341 ttttataatc gtctagtaga aattgctagg cctgggaaaa tcccgcaaac attcacgtac
    14401 acacagcaaa aaaatctcta tgaagaagta ttgtattccc gcgatctgtc tgagttttcg
    14461 ccatgggaag aggtaaggct ccatgaactg ttaagtacaa aaccagtttc attgaatttt
    14521 tttaatgcat tagataacaa aagaaaatta atttcaacaa attattggga agtatttgct
    14581 gcaagaaaaa aatggatgga agaaaagaag attgccgatc agcagatcca gtttgtccag
    14641 ctgaaaaaag aaaaactata ttatgggtta tacagccgtt aacagcaagc gaggaagacc
    14701 atgacattaa aagaagaaat catacaagaa agcaaacgat taggaattga caaaatcggg
    14761 ttcaccacgg cggaaccttt tgattcatta aaagaatcgt tggaagaaca aaaagcagct
    14821 ggccatactt ctggctttga acatccaaat atcgatgagc gtctttatcc agaaatgaca
    14881 tttgatcagc cgaaatcgat catttccatt gcacttgcgt atcctactag gatccatgaa
    14941 gaagtgccta gggatgaaaa acgcgggcag tttgctcgtg cttcttgggg gatcgattac
    15001 catgatatac tcagagaccg tttgaaccga ttgatcgaat tcatccaaac aagagcagaa
    15061 aaatggcaaa aagaagagga atggcgtcta gctccgcaag tagatactgg agaactgatc
    15121 gatgtcgctg tagcacagcg agctggttta ggatttattg gacgaaacgg attgttgatc
    15181 actgaagagt tcggttcatt tgtctatctt ggtgagatcg ttactaatat tgtatttgag
    15241 cctgataaac caggagaatt tggttgcggt gactgtatgc gctgcgtgac tgcttgtccg
    15301 acaaaagcat tgcttggtga cggaagaatg aatgcccagc gatgtttgtc ttatcaaact
    15361 cagacaaaag ggatgatgcc ggaagaatat cgtaaaaaaa tgcgaaatgt catttatgga
    15421 tgtgatatct gccagttagt ctgcccttac aatcaaggaa aagattttca ttttcatgaa
    15481 gagatggaac caaaagtaga cgaaatatat ccaaaattaa agccaatgct ttcgatgtct
    15541 aataaagact tcaaacagca atttgggcat ttagctggtt catggcgtgg gaagaaacct
    15601 ttgcagagaa acgcattgat tgctttagct aatctaggtg atcgaacggc attgcctgag
    15661 atttcagaat gtttaacaga tgtccgccct gtcatcagag gaacagcagc ttgggctatt
    15721 ggacgtctag gaacaaaaga accggaaaaa tggcttgatg tattagatga aatattagct
    15781 aaagaaacag aagaagtcgt tataaacgaa ttggaacatg cccagaaatt attgcggaga
    15841 aagttaaaat aaaaaaatag ttaacattat tcaaagtttt tgtccagtaa ttgttgtaac
    15901 aattactggt ttttttgtat tttttaca
//
LOCUS       DASJSZ010000062        15577 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.063, whole genome shotgun sequence.
ACCESSION   DASJSZ010000062 DASJSZ010000000
VERSION     DASJSZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.063"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            182..1369
                     /locus_tag="U4B03_001939"
     CDS             182..1369
                     /locus_tag="U4B03_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2068783.1"
                     /translation="MQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKK
                     QVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIES
                     VTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWEN
                     TDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFE
                     SHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWS
                     SQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFD
                     FQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAV
                     QNK"
     gene            complement(1461..1826)
                     /locus_tag="U4B03_001940"
     CDS             complement(1461..1826)
                     /locus_tag="U4B03_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN2068784.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1823..2188)
                     /locus_tag="U4B03_001941"
     CDS             complement(1823..2188)
                     /locus_tag="U4B03_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN2068785.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2380..2628)
                     /locus_tag="U4B03_001942"
     CDS             complement(2380..2628)
                     /locus_tag="U4B03_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068786.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2627..2755
                     /locus_tag="U4B03_001943"
     CDS             2627..2755
                     /locus_tag="U4B03_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEN2068787.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2812..4506
                     /locus_tag="U4B03_001944"
     CDS             2812..4506
                     /locus_tag="U4B03_001944"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEN2068788.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4510..4629
                     /locus_tag="U4B03_001945"
                     /pseudo
     CDS             4510..4629
                     /locus_tag="U4B03_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4606..4779
                     /locus_tag="U4B03_001946"
     CDS             4606..4779
                     /locus_tag="U4B03_001946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068789.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5157..6290
                     /locus_tag="U4B03_001947"
     CDS             5157..6290
                     /locus_tag="U4B03_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEN2068790.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6321..6641
                     /locus_tag="U4B03_001948"
     CDS             6321..6641
                     /locus_tag="U4B03_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEN2068791.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6670..7134)
                     /locus_tag="U4B03_001949"
     CDS             complement(6670..7134)
                     /locus_tag="U4B03_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2068792.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7266..8519)
                     /locus_tag="U4B03_001950"
     CDS             complement(7266..8519)
                     /locus_tag="U4B03_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2068793.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8548..8865)
                     /locus_tag="U4B03_001951"
     CDS             complement(8548..8865)
                     /locus_tag="U4B03_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2068794.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8891..9211)
                     /locus_tag="U4B03_001952"
     CDS             complement(8891..9211)
                     /locus_tag="U4B03_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2068795.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9675..11090)
                     /locus_tag="U4B03_001953"
     CDS             complement(9675..11090)
                     /locus_tag="U4B03_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2068796.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11107..12423)
                     /locus_tag="U4B03_001954"
     CDS             complement(11107..12423)
                     /locus_tag="U4B03_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2068797.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12573..13271)
                     /locus_tag="U4B03_001955"
     CDS             complement(12573..13271)
                     /locus_tag="U4B03_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEN2068798.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13560..14516)
                     /locus_tag="U4B03_001956"
     CDS             complement(13560..14516)
                     /locus_tag="U4B03_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2068799.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14629..15462)
                     /gene="msrA"
                     /locus_tag="U4B03_001957"
     CDS             complement(14629..15462)
                     /gene="msrA"
                     /locus_tag="U4B03_001957"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2068800.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4836 a         3169 c         2667 g         4905 t
ORIGIN      
        1 ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg tttgcaaaac
       61 cactttactc aatgatatca ataacataaa aaaagaattt gaagaacaga tcgcttatac
      121 acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc gatgcaagat
      181 catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt atttggaaga
      241 agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca gcaaagctta
      301 ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa tcgaccgcta
      361 ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt cgtatcgttt
      421 aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg atcgtttcat
      481 tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag aaatcttatc
      541 gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa tagattcaaa
      601 atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag catgggaaaa
      661 cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga cactattcaa
      721 tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc aacagctaca
      781 ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct ttgaatctca
      841 cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt gcgctgattc atctgatgcg
      901 ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttt taaatgagga
      961 acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca gccagttacc
     1021 ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat tgagcggaat
     1081 tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg atgttcatta
     1141 tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag ttgcaccaac
     1201 aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa accgggtatt
     1261 atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta ttcctatttc
     1321 gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag gggctgtgac
     1381 aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac aatctcttga
     1441 tacacgtcag gctcacaacc tcacttattt tggacatcta cgggtccgat ctctttattt
     1501 aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa aatatcctgc
     1561 aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc caaacttaga
     1621 agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg gtcgctttgc
     1681 acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca aacaccccaa
     1741 accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcgaa atatggaaga
     1801 agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt tctttgtctt
     1861 ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata agtgcaagct
     1921 tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca acgactagta
     1981 cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca ttacgagtat
     2041 tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat agaacaagaa
     2101 cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg atccattcta
     2161 gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta atttgttcct
     2221 attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt tctaatgata
     2281 acggttcgct taaatttttc tttttcactc tcccattttt ctccctccat ccataaatcg
     2341 tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca tcagcttttc
     2401 aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag aaccatacac
     2461 ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat cagacacttc
     2521 ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt ctaataaaaa
     2581 cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga gtaaaaaatt
     2641 aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc taggaaaaaa
     2701 gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa tctagcatcc
     2761 tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac aatgagaaaa
     2821 agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt ttatttgatt
     2881 caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga tcacggttca
     2941 aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa ccatccatta
     3001 gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg tacctatgta
     3061 gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat gactgctgaa
     3121 gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg tttgatggaa
     3181 ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga ccgtattctt
     3241 ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa tatcactatc
     3301 gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt tatgtgggaa
     3361 acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta tatgcatcaa
     3421 atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac acgttacaat
     3481 caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg atgcaaaatc
     3541 attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca agatgaaatc
     3601 actgtgactg gtttgaaaat taccaataca ctgactcaag aagaagaata cgttgtggtc
     3661 gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc tactttagga
     3721 gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag tctctggaaa
     3781 aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc tgatcgagat
     3841 gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt gaatgaaatc
     3901 acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc aaccacaccg
     3961 atcacgccgt taggccaaaa agatgtcaat atgtctattg tcgtgcatca tcaaccgcat
     4021 tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta tccaagacga
     4081 cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat gctagaagaa
     4141 ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga aaaagaaaca
     4201 gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc ttccgcgttg
     4261 ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc gaccaatctg
     4321 gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga acaaagtgct
     4381 gtacgttctg gagaaatcgc tgctttccat tttgcgggta ttcctatgtc ccgtcttgtc
     4441 aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa aaaaatgttt
     4501 gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac aaccaggcta
     4561 aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca agcttttgaa
     4621 caccgtttac caagaggtta tgaaaaagct gccctgcatc aagtaataag agcagccaag
     4681 ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat gcaagctttt
     4741 gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg aagaaaaatt
     4801 ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag aaactgatcc
     4861 cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac tattttttca
     4921 acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc aatgattacc
     4981 attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga ctatagaatg
     5041 tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt taaaatgaaa
     5101 ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca tctccaatga
     5161 aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa
     5221 tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc ggctccgatc
     5281 tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta ggacacgaac
     5341 caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg
     5401 tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat cacatggaaa
     5461 gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga tttggcaagt
     5521 tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat gcagactcta
     5581 cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt ctatcagata
     5641 ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg
     5701 tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt atggctggag
     5761 cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg gctaattaca
     5821 atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa ttgtatgaac
     5881 tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga ttagagcctg
     5941 tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct ccattacaga
     6001 tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt tatatgactc
     6061 cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc aagcatggac
     6121 aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa ggactattcg
     6181 atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa gcatatgaga
     6241 tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga attcagtaaa
     6301 aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag
     6361 gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc caaaatcgtt
     6421 acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt tttggaagcc
     6481 ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg gctggtgcta
     6541 ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt tacgacgaaa
     6601 aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc aaatctcatg
     6661 cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc cactttcttc
     6721 cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat gatctgtaat
     6781 taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg ctaatatccg
     6841 cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa gatttggtgt
     6901 cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga cgatggtttc
     6961 tttttcatct aagaccacat accattgagg aatactttcc ttattagcga ttccagtacg
     7021 aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc aattcgcgct
     7081 ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa ccaatgttta
     7141 aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga aaattagcaa
     7201 tgacaacaat agtgatgtaa aaaaaaaaac ggaaaacaat cacttgttct ccgttttttg
     7261 attaatcaag catctaaagc ttcttttctt ctttcttgga tcgtagctac cttgacgaac
     7321 ggtagataga tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc acctgcaata
     7381 ctttttgttg ccaatacacc gccaataatt ggtggagtta cccatggagg cataacggta
     7441 gctgctggca ctaaacctgt agaagtcgca aagtaagcaa cactaactaa gaccattggt
     7501 gtcaaaataa atggaatgaa catgatcggg ttcaacacga taggtaaccc aaacatcgtt
     7561 ggttcattaa tattgaagac acctggcgca atcgacaagt ttgttacaac catatatggt
     7621 ttgtttttac gtcctactaa ataaatagct aacagtaaac ctaaagttgc acctgttcct
     7681 cctaagttga caaagctgtc aaagaaaggt ttgttcacga taaacggtgc gctatgtcca
     7741 gctgcgattg cattaacgtt tgcttcaatg gctggcgcat tgattgtttg catcaaagga
     7801 tcgaccatat tcgctccgtg taaaccaaag aaccataaga atggtgtgat aaatgcaagc
     7861 agtaaggcag atggataaga gtttgcaagt cccataaatg gttcttggac cactgtatag
     7921 aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac taaagcaaac
     7981 gcagagatag tgatcattgc tggtagtaat gaagcaaatg cttttgcaac tgccggtggc
     8041 acaccatcag gcattttgat gatcaatttt tcatttccgc ttaaacgtac aaagatttct
     8101 gttgaaataa tcccaacaat aatagcaata aacaaaccgt ttgagctcat acctaatgct
     8161 ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg cggaaacacc gtcttttcgg
     8221 tagccgttag ctaaatgata agcaagcaag aaagcaatca agacagaaag aatggcaaat
     8281 gtaccgttcc aagcagcagc cccgaatcct ttccatgatt caccaccaaa aattgagttc
     8341 atcaattctt ggaacccggg aatcggtaag ttattgatca ttactgctaa tgctcctaag
     8401 atcattaacg gcatactcac cataaatgag tcccgaatcg caactaaatg ccgttgtgca
     8461 ccaatttttg atgctacagg tataaaatgt ttctccataa atgcaacaaa tttatccata
     8521 aatcttccct cttttcttta ctgtagatta cgcgttcaat cgacgataca gatcaatgat
     8581 ctcttttgct aaatctgtaa aagctataga tgtcatcaga tgatcttgac tgtggaccgt
     8641 caaaagcgtg acttcaacgt gatttccttg tgcttcttgt gtcagcatct ctgtttgaga
     8701 gtgatgtgct tggactaaag attcctctgc atctttgatt ttttcatctg ctaaagtgaa
     8761 atcacctttt ttcgcagcag caatcgcttc cattgcatcg ctttttgcat ttccgccaaa
     8821 catgatcaga cccatgatgg cttctagatt tttttgatct tccatttatt gtatcctccg
     8881 ataattttta ttatttgttt attagttttt ctgcttggtc gagtactttt tcaccattca
     8941 tcatgccata gtcagccata ttgattacat ctaaaggaat gcctttaggt gctagctttt
     9001 cagcaaattg attttgcata aagcgtactt gcggtcctaa aagtaaaacg tctacatttt
     9061 tagattctag ttgattatcc gcatctgaag cagatacagc aaagatgtct gcttccattc
     9121 cttttgcttc tgctgccttt tgcatttttg tgactaggag acttgtactc attccagctg
     9181 aacatacaag catgattgtt ttcttttcca ttttaaacac gttcctttct atacttacac
     9241 ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg atatcatcac
     9301 atttaaggtc tataccaagt tgcatacaca ctaaaaaaga aagcgcacac taaacacaca
     9361 aattaatttt tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg acacaaaact
     9421 tgtcacaact tttttcaaat aaacggtatt caaaagcttg catctgcggt aataagtcca
     9481 accaagttaa aaacataaga tgttgttttt actttgctat tcttattact tgatgcagga
     9541 cagctttttc acaacctctc aataaacggt gttcaatcag ctgaccctgg gtattagctc
     9601 aaaacgctgg attcacaacc tcttttattt caaataacca gtaaatacaa tgaacatcta
     9661 caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct cttatcaatc
     9721 aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc gttcaattcg
     9781 attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca gtcagtgaat
     9841 gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat caagtaatgc
     9901 aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt ctcatccata
     9961 tactgttctt catattctct tcccataccg ttttctgtta tcagccaagg gatattatga
    10021 taattttctt ttagatacat ggctatatca tacatgattt tcggatagat ttcccatccc
    10081 ctggaattat tcattttctt cccaggtaaa tcaaagactt cataatatcg ttcaggatgg
    10141 aaaggtgtct cttcattcca ttgataacga ggggcgcgaa cgcgtttagg ataatattga
    10201 ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc tgttgcagga
    10261 tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta gtatcctttt
    10321 accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc agctttttgg
    10381 tcttctggag aattggatcg agcatagacc atttcaggat ttaaaataca gccaatcctt
    10441 ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa aactttatga
    10501 taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac agcatccaaa
    10561 tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt taccctatca
    10621 ccgtaacgat caaaggcaat ttgagcatac ttagcataaa aatcaactac ttttcttgaa
    10681 gaccagccat catatttttc caaaagaaaa gctgggactt cataatgttc caaacaaatc
    10741 atcggttcta cgtctgcttc gatcaaagag tcgatcattt cacttatatg ctgagcatat
    10801 tcttcatcta ctatacagtt ctcataatca gtaaaaaaac gagcccaatt gatagaagtc
    10861 cgataatgtg tcagatggat ggatttcatc agatccacat cctgctggta ttgttccata
    10921 aaattagttg ctacggctgg gccataccca ttatgccaaa caaaatgttc atttttatac
    10981 cagctatcta taaacgaatc ctgtccttct ttttttcctt tccaaccttc cgtttgccaa
    11041 gcagaagatg cagcaccaag aataaaattt tttggaatag taattttcat gatttcctcc
    11101 tgttttttag atcccatcat tttccacacg atttgctact tttacaaatg gcagatagat
    11161 caaaatagaa acaataatac aaacaagttg cgtaataact gcaccgatat ttcctgctgt
    11221 tgccagataa gcattgacga tcggcggcat tggccaaggg accattacaa ctgctttcgc
    11281 cgcaaatcct aaagcggttg caaaataacc aatcgttcca gtgataagag gcgttaagat
    11341 aaacggaata gctaaaattg gattcagcat gatcggcata ccaaaaataa ctggttcatt
    11401 gatgttgaag atcccaggag caagggataa cttggtgatt tgtttcatat cttcccgttt
    11461 tccaactaac aatactgcaa tcagtaaacc gatcgtgact ccagacccgc ccatactcat
    11521 atacatatcc caaaatggca tagtaataat gttcggtact tcttttccag cttcaaaagc
    11581 ttccgtattt acagcaattg ccgctaaaag caacggttcc cgtatcggct tcaccatttg
    11641 atttccatgg ataccaatca cccaaaagac ttgcgcaata aacatcaata gcaaaatacc
    11701 cggtaaccct tgtacgacac cttctaatgg gcgttgtacg acattgtaaa tgatatcata
    11761 aaggtacatt cctgtaagtt ctttgatgat aaatcccgca gccgcgatac ccgatacagt
    11821 cagaatcgtc gggaataaag cagaaaaact agcagaaaca ttcgttggca cggtatcagg
    11881 cattctcagc tgtagtttct tctgtttcct caaccagcaa taaagttcaa tagaaaggat
    11941 ggagaccaac attcccaaaa ataacccttt cgtatctgta tattgtttag ccaatacgtt
    12001 ttctactata acagtctgat tttcattcac taattcataa acagttggat taacagataa
    12061 ataagacatc actgcgatga taccagggaa tacaccatcc actttattca gcttggctaa
    12121 ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga catagctgat
    12181 ggcagatgcg ataggcttta attcttctaa aaaacgcaag aaacttatct gtgctaagcc
    12241 attttccgag tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg taataattac
    12301 cggaatcaat gcagcaaatg cgttttttat caccatgata taccttaaat tattgattct
    12361 cgttgccacg ttccctaaaa cattggctaa tttatccata aaactcattc cagatcccct
    12421 cattcctgct tcttctcctt gacgtcatct ctttttgtct attagtcagc gcttacattt
    12481 cgatgaacag aaactattta ctcctctttt ttaccaaata taaaacaaaa attcatctga
    12541 tgtatttatc ataaaataca tcagatgaac tgtcaattag tttctttttc tcctattatt
    12601 ttctgtaatt ctctccgatt tattgccata tggacagtca ttgcatcaaa cgcagcaagt
    12661 gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt ctcgattttc
    12721 agatgctgga tatcataatt tttattaaac aaagcaattg attcattaat gattgggata
    12781 atatgcagca aggctacgtt tccgctagct tctgcaatca tcgtatgaaa atcgatgtct
    12841 aattgaacat gttcttgtgt gccttctcga aaagaagttt caatcttttg cgttaatttt
    12901 ttcatttgga cgatttgatt cttcgttgca tattccgctg ccaaacctgc catcttaggc
    12961 tccaataaat atctcagttc aaataagtct cgtatcatcc gatcctgatc ttcaatcaaa
    13021 gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt ccccgctcct
    13081 tgccgaactt caaggatatt cctggaagct agagcccgca cagcttctcg caccgtgctt
    13141 cgccccacct ctaaataatc ggacaattca tattcattcg gcagtttatc tcctacttgc
    13201 aactgcattt cctttaaata tctaaggatc ttgtcagtag ttacttctac tagggtttta
    13261 ttttcttcca aaccacatac ctccttttca tctgatgtat tttttcgtaa ttattataac
    13321 acgtttgaat catctgatga ttttttgtat acaaaattga ttaatccgtt catagacata
    13381 caaacgaaga agatatttac cataaaaaag actaggataa aattcactcc tagccttcga
    13441 tcatttttat tttgaacgac aaaaaatatg atgttcaata acagacgatc ttcattcgat
    13501 tcttgatgtc atcttttgtt ttttttaata tatggataaa aagtttgatt acatcgtttc
    13561 tattctagaa tttggtcgat tctcaattct tcttctggtg agaaatctaa attatccatc
    13621 attcgaacac tttcttgtaa ctgacttaac cgacttgctc cgaccagtac acttgtaaca
    13681 gatttttgtc gcaaattcca tgcaagggcc atttgcgcta atgattgtcc tcgtgatacg
    13741 gcgattgttt gcagtgcttt gattttttcc agagttgatc caacttgttc ttctgaaagg
    13801 aatggtattt ccttacgatg tgctctagaa tcttcaggaa taccatgtag ataacgattt
    13861 gtcagcaacc cttgagcaag cggactgaac gtgattgccc ctagcccttc ttcttctaat
    13921 actggagata gatcgtcttc tagtagtgca cgagaaaaca tattgtagcg catctgatga
    13981 ataatgaacg gtgcttcctt tctttttaaa atttcagtca ttttttttgt atcttctcca
    14041 ttatagttgg atataccaat atataatgct tttccttgtc ttacaagttg catcaaagct
    14101 tctgctgttt cttccaaagg tgtatcaggg tctgggcgat gatgatagaa gatgtcaaca
    14161 taatctagcc ccattctttg gagactttgg tcacaacttg aaatgatgga tttacgagag
    14221 ccccattccc catatggccc ttcccacata tgataacctg ctttgctaga aataatcagt
    14281 tcatcacggt aggcttgaaa atcttctcta aaaatacgcc caaagttttt ctctgcagct
    14341 ccagcagggc ctccataatt attcgcaagg tcgaaatggg taattcccat gtcgaatgcg
    14401 cctcttaaaa tctctctttg attatggata ggatcataat caccaaaatt ttgccataat
    14461 ccgagagata aaatcggtaa tttcaaacct gaatgaccgg atttacgata ttccattttt
    14521 tcataacgtg cactatctgc aatatacaat ttttctcctc ctcatcaaat ctttttaaaa
    14581 cgctttcttt tttctatttt tagtttacta cattttaaag aaaaggagtt aatcaaaatg
    14641 attttttcgt atattttttt gccatttgtt ttttactcgt tgatagatca gaaattgatt
    14701 acgtgcttgc tggatttttt tataacgttt cggttgtttt ttgtaaaatt gctgatgata
    14761 gttttctgct ggccaaaacg taccggctgg ttcgattttt gtgacgattg gttggtggaa
    14821 agttcctgaa ctgtctagtt tctgttttga ttgttccgct aattcttttt gtcgttcgac
    14881 ttgataaaaa atcactggac gatattgctt ccctctatct tgaaattgtc caaatgtatc
    14941 tgtgggatca gtaatttgcc aatagattgt cagcaactcg ctataactta tttctcttgt
    15001 gtcataaatg atctccacag cttccacatg acctgtgtaa ccaccactta cttgatcata
    15061 atttggtttt tcaacatgtc cgcccgtgta tcctgacaaa acagatagga tccctttttt
    15121 cgattcaaac ggctcgacca tacaccagaa acatccccct gcaaatactg cacgttcttg
    15181 atagtcagca tcttgcatgg catgcttgga aaagtcatat ttttgctcct tatcaaatcc
    15241 ttccgtaatc aataaataaa agtccattac atcaggcgtc agattcattc ggatagctaa
    15301 tggacgtagt gttgcttcta gttttgataa ctgttcttta acggatatat tttctcttat
    15361 gtcattcttt gtctgaatca atacttttcg ttcccaatct cgtgtattcg gattcaaaat
    15421 cagattataa agtgtttgaa tgaatatttg ttcctcatcc atagattctc ctcctttccg
    15481 aagaagacaa gctagtgact ttagctgtaa tttatcataa agtgaggaaa taagcgatta
    15541 cttcatcccc ctacagattt ttcgtataaa aaagaga
//
LOCUS       DASJSZ010000063        15322 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.064, whole genome shotgun sequence.
ACCESSION   DASJSZ010000063 DASJSZ010000000
VERSION     DASJSZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.064"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(113..571)
                     /locus_tag="U4B03_001958"
     CDS             complement(113..571)
                     /locus_tag="U4B03_001958"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEN2068801.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(742..1887)
                     /locus_tag="U4B03_001959"
     CDS             complement(742..1887)
                     /locus_tag="U4B03_001959"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEN2068802.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1900..2817)
                     /locus_tag="U4B03_001960"
     CDS             complement(1900..2817)
                     /locus_tag="U4B03_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEN2068803.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3245..3808
                     /gene="efp"
                     /locus_tag="U4B03_001961"
     CDS             3245..3808
                     /gene="efp"
                     /locus_tag="U4B03_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEN2068804.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            3909..4079
                     /locus_tag="U4B03_001962"
     CDS             3909..4079
                     /locus_tag="U4B03_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068805.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4294..5553
                     /gene="lysA"
                     /locus_tag="U4B03_001963"
     CDS             4294..5553
                     /gene="lysA"
                     /locus_tag="U4B03_001963"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEN2068806.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            5540..6340
                     /gene="dapF"
                     /locus_tag="U4B03_001964"
     CDS             5540..6340
                     /gene="dapF"
                     /locus_tag="U4B03_001964"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEN2068807.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6343..7224
                     /locus_tag="U4B03_001965"
     CDS             6343..7224
                     /locus_tag="U4B03_001965"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEN2068808.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7221..7937
                     /locus_tag="U4B03_001966"
     CDS             7221..7937
                     /locus_tag="U4B03_001966"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEN2068809.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            7943..8638
                     /gene="dapD"
                     /locus_tag="U4B03_001967"
     CDS             7943..8638
                     /gene="dapD"
                     /locus_tag="U4B03_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEN2068810.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            8654..9655
                     /locus_tag="U4B03_001968"
     CDS             8654..9655
                     /locus_tag="U4B03_001968"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEN2068811.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            9652..11013
                     /locus_tag="U4B03_001969"
     CDS             9652..11013
                     /locus_tag="U4B03_001969"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEN2068812.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            10973..12124
                     /locus_tag="U4B03_001970"
     CDS             10973..12124
                     /locus_tag="U4B03_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEN2068813.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12099..13268
                     /locus_tag="U4B03_001971"
     CDS             12099..13268
                     /locus_tag="U4B03_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2068814.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13443..14822
                     /locus_tag="U4B03_001972"
     CDS             13443..14822
                     /locus_tag="U4B03_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2068815.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15103..15322
                     /locus_tag="U4B03_001973"
                     /pseudo
     CDS             15103..15322
                     /locus_tag="U4B03_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5019 a         2719 c         3278 g         4306 t
ORIGIN      
        1 gaaaactagc tatttgtttt cccgtttaga actcttttca gacaataaat atttaataaa
       61 atacgaagat aagatagcta tattgcttct catctttacg ttcttttaga aactatgctt
      121 ctacttcaga ccattcatcg cgttttgcta aaaattcacg agccaaagct tgtgcacctt
      181 ctagtgtatg gctggcagcc cagccacatt gtgtttcatt gcttgcaggt acttcagtag
      241 cttcaagaac atctttcatt gttgcttcaa gaacatctaa aatgtcttcg tagctttcat
      301 gattcaatac agtcaagtaa aagcctgttt ggcatcccat tggagaccag tctacgatgt
      361 aatctgcgtg attgcggatt agttcggcag tcaagtgctc aagtgaatgt agacttggca
      421 tgtccatgtg ttctttattt ggttgtttaa agcggacgtc gtatttaatg atgacatcgc
      481 catgcgcacc cgtttttcga tcagctacac gtacataagg tgctttgact tttgtatggt
      541 caagattaaa actttcgaca ttcattctca tttgtaagac tcctatctgt ttgttcattc
      601 gttttgatgc ttttcactaa agaattattt agtgaaattt acaaaaacgt ctttctactt
      661 gattctaaca cttatcgttt ccaaggtgta gcaatctgaa atatttttta gatttttctg
      721 ctaagtcgtt tattgggact tctagttctt gattctgtca aatgcctgag ctaaatcgtt
      781 tatgagatcc tgaccatact caattcctac agaaagacgg atcaagccgt cctttatgcc
      841 agcctcttct cgtttttctt ttggaataga cgcatgagtc atgacagaag gaatttccac
      901 taaactttct acgccaccta aactttctgc taaagtaaat agttggagtg attcaacaaa
      961 tggaatagca gattcttcat tttttaaagt aaaagaaatc attccgctaa agccgctcat
     1021 ctgctgctta gcaactccat ggagttcatg atcgggtaat cctggatagt agaccttttc
     1081 tactgctgga tgagaaaata gaaaatcagc taccacaaaa gcattttttt gatgttcctc
     1141 catacgaaca cttagtgtct tgattccgcg ctgaagtaac cagctatctt ggggaccaag
     1201 tacggctccg atagcatttt ggtaaaatcc gatctgatcg gctagatctt tatggttaga
     1261 agtcactaaa ccagctacta catcgctgtg tccgcctaga tatttggtac cgctatggac
     1321 aacgatatca gaaccgagat ctaaaggtct ttgaaaataa ggagtagcaa atgtattgtc
     1381 agcaataact atcaatccat gttgatgagc cagagtggca cttttttcta aatcagtaat
     1441 ctttagaagc ggattgctag gtgtttcaag ataaagtgct tttgtattgg gtttgatgga
     1501 ttgttctatt ttatcaagat tactcgtatc tatgatcgtg tactcgagac catttttagt
     1561 aagaactttg tcaaatagac ggaatgttcc tccatagaca tcatctccta ataagatatg
     1621 atcgccagct tgaaaaagag aaaaaacggc atgaatccca gaaagaccag aagaaaaagc
     1681 gaatcctcga acaccgcctt ccaaatcagc aatcagttct tccaatgcaa aacgagtagg
     1741 attaccagat cttgaatatt catattgttt aggctggccg acgccatctt gccgataagt
     1801 ggatgtttga taaataggta cactgacagc tcctgttgtg ggatcttcac tgatgcctcc
     1861 gtggatcaat tttgtttgaa tgtgcatgtt tctttcctcc tataagtaaa tgttttttga
     1921 taaatagcgg tctgctgcgt ctggaaaaat cgtaacgaca cgatgaccag gtggtaaacg
     1981 acgaatttct cttaatgctg ctgcaaaagc cgcaccactc gaacttccaa ctaatagtcc
     2041 ttgttctcgt gctaactgcc tagtatagtt aaacccttct acatcagaga ttgtctcgat
     2101 ctgattgatt gaaaggggag aaagaaacgg cggaatgaat tctacaccga taccctcaat
     2161 ttcgtgaggg gctggatcac caccatttaa cacagagccc tctggttcta caccgattat
     2221 tcgaatgtcg ggatattttt ctttcaaaaa ggtagaagtt ccagcaaagg ttccaccact
     2281 cccaattcct gctacaaaac tatggatatt ttctttgatt tcatgaaaga tttctggccc
     2341 taacgtatga tagtaagcag caggattatc tttattttca aattgaagag gcagatagct
     2401 gtttgatatc tcctctgcaa gttttttact tttgctgatg gcaccgatga ttccttgctc
     2461 actaggagaa tggatgatct ttgctcccaa tgctttcatc aattgttgtt tttctaagct
     2521 aaacttctct ggaacaacaa aaaccgttct taatttgtat gtgagtgcgg ccagagcgat
     2581 cccaattcct gtatttccgg cagttggttc gatgatcgtc gttttttcat tgatttttcc
     2641 ttgggcaaat ccttctttga tcaaataact gcccaatcta tctttgatac ttcctccagg
     2701 atttaaatat tctagttttg cataaatagc agaaccttta ggcatagaat agtgctgatt
     2761 gtcaaagcga taaagcggtg tggaaccaat agcttctgta attgtcgaga taatcatgtg
     2821 ggcctcctaa aaaaagataa taaaaaaagg agctgactat taaatcagct ccttgaaaga
     2881 catagagaaa aattgagtag ataaaaagat aagttacctg gtactcattc tatgaagggc
     2941 aattaataga catgacaaat agtatgctgc ttttatgcac catacaacag caacgattca
     3001 attgtttttg gttgctgact gtcatgtgta tccctccaag taaaattttt attaacgata
     3061 gcatgaaaaa aaataaaagt caaaggcttg aaagaaaaaa atatttcgat agcatcgttt
     3121 acccgtgttt atttttctga caaataggca tgagacctta aaagatagag aatactctag
     3181 ttttctggcc ggttgtctga tacaataaag ttgattatga taaagaaaag aaggagcaaa
     3241 aataatgatc tcagcaagtg atttaagagc cggtatgact ttcgtacaag acggcaaact
     3301 gattaaagtt ctggatgcaa gccaccataa acctggtaaa ggaaatacag ttatgcgtat
     3361 gaaactgaaa gatgtccgtt caggtgcaac atacgacaca acgttcagac cagaagaaaa
     3421 atttgaaaaa gcacatatcg acacaaaacc tgtacaatat ttgtacacga tggatgatac
     3481 agctttcttc atggatttgg aaacatatga gcaatatgaa attcctgtag aaacagtaga
     3541 agaagaaatg aaatatatcc tagaaaacat ggaagtaaaa atccaatttt tcggtagcga
     3601 agtcatcggc gttcaattac caacaactgt cgtattacgt gttgtggaaa cacaaccttc
     3661 catcaaaggt gcaacagtca ctggttcagg taagccagcg acaatggaaa ctggcttagt
     3721 agtcaatgtt cctgacttca tcgaaacaga tgaattgttg gaaatcaata cagcagaagg
     3781 ttcttatgta aaacgtgcgt caaaataatt cgctaacgga ttgataagaa tttgaaaaca
     3841 gagcttgacc tcttgaaagg cagatcatcg gatctgcctt tcaagaggtt ttttgtggaa
     3901 tcagcaaatt gaaaaataaa aaagtatttg attttattta tattttgaaa gatgaatggc
     3961 aaaagctaga gaaagaaaaa actgcactta tttatgaaga aattgaaatg attcataaaa
     4021 ttgaaaatat ccgaactatt ccgaactata ttgaagcgaa aatatttttt catcactaaa
     4081 tagtgttgaa tttatgtttt tatgtataaa tagcttctaa tagttcgggt attttgtttt
     4141 tataaccatt tttattctta tttttaaaaa aatatttcga aatatactag acaattcaga
     4201 aaattttgtt taatattatt ccaataaaat tcaaatcttt atcaaatgaa attccaataa
     4261 aaacggaaaa aaagtgaaaa gaggaatgaa aaaatgacag aattgataat tggcggagtc
     4321 acagcaagca cattggcaga aaagtataag acacctctgt atgtgtatga tgaagaaaag
     4381 atgagacaaa cgatgctgtc attcaagcga ggctttgtat cgaaacgttt tgatacaaaa
     4441 gtgctttacg cctctaaagc attccaaacg attgagatgc tgcatttagt tcgtgaatat
     4501 ggattcgggc tagatgttgt cagtggtggg gaaatctata ccgctttgca agctgatttt
     4561 cctagagata ctatctactt ccatggtaac aacaagacgc cagaggaact gatctatgca
     4621 cttgaaaatg atttgttgca tattgttgcc gataatttga tggaagttga attattagca
     4681 gaattaagcc aacgctattt aaaaaaaatc tgtatcatgc ttcggttgaa tgtcggagta
     4741 gaagcacata ctcatgaata tatcgtcact gcgcatattg attcaaaatt cggcatgagc
     4801 tatgaaagta aagagtgcca acaagccctt cagttgataa aagaaagcag ctatctagag
     4861 ttagaaggat ttcatgctca tatcgggtca caaatttttg atgttactgc ttggttagct
     4921 gaaatcgaca aattgacgaa ttacttaacc gattttgacc aacctctttc cttgaatctt
     4981 ggcggagggt ttgggattcg ttatacacaa aatgatcagc cgatcccagt cgaagaggca
     5041 ctaaaaaaac tgattgaata tacggaagaa gctttggaaa aacgaaaact gaccattcgt
     5101 caattactga tcgaaccggg tagaagtatt gttggagaag ctggtacaac actatacacg
     5161 attggtttca tcaaaaagac accacataaa aaatattact ttgtagatgg cgggatgaca
     5221 gataatatcc gtccagctct gtatcaggca gaatacgatt gtgatttagc agcaaaactg
     5281 tcggaagaaa aaacagagaa agtgacgatt gcaggaaaaa tgtgtgaatc aggcgatgtg
     5341 gtaattaaag aagcttattt gccgaaagca gaatcgggtg atcttttagc tgtttattcc
     5401 acgggagctt atggatactc gatgagcagc aactacaatc gagcgacacg accagcagtt
     5461 gtttttgtca ataatggcca atcaagactg attgttcgca gacaagcatt ttctgatcta
     5521 ctaagggggg aagtttccta tgatccttca taaatataat ggctgcggaa atgattttat
     5581 cttattagat tacgcagaag acacagatta tagtcaatta gccgcttctt tatgcacaaa
     5641 agaaaaatat gatatagacg gattgatcgc cgtaaaaatc gatccgttgg aaatgatcta
     5701 ttacaacaaa gatggcagcc gcgcgccgat gtgcggaaat ggcattcgct gttttgctcg
     5761 atacgtaaat gatcagaaca tgatgaatga acaggaattc aacgtagaga cattagcagg
     5821 cacaagaaaa attacggtgt tggatccctc acctttttat tgcagcgtag atatgggagt
     5881 gccagatgat tctccggaaa aaatcaaagc taatcaaact tatccaataa aaaaccagga
     5941 aatagagatc aatggtgaaa aagtaacgat cactagttta tttttaggaa cgattcatac
     6001 cgttgttttt gttgaaaatg cgcgtgagga agtctggaga aaaagaggtg aactgatctg
     6061 tcatcatcct ttgttcaaag aaaaaaccaa tgtcaacttt gtagaagtag tcaatgaaca
     6121 agaattgatc gtccgtacct atgaaagagg agtaggttgg acacttgctt gcggtacagg
     6181 ttgctgtgcc tcttatgtgg ttgctaagga aactgggaaa atttcagcag atcaggtagt
     6241 tgttcacttg gaacaaggag acttgatgat ctctggtgag gatgcgatac aaatggctgg
     6301 cccagcagtt catgaatgga caagaatatt ggaggaatga agatgataaa aggttcgatc
     6361 gtggcgctga tcacgccgat gaatgaagag ggatcagtag attatgctgg tttggaaaaa
     6421 ttgatccaat tccatttaga tgaacaaaca gatggattat tagttttagg tacaacagga
     6481 gaatcttcta cgctcacaca atctgaggag gagcagatcc ttcaactgac agtaaagaaa
     6541 gtggctggcc gtgtgccggt gattgcagga gctggaacga ataatacgaa agaaacgatt
     6601 gaaaaagcga agcattttgc ttctttgggg gcagacgcac ttttggtcat caccccttac
     6661 tacaacaaaa caagtgatgc aggactagct gctcatttta cagcaattgc agaagcctca
     6721 ccgattccgc ttattttgta taatgttcct tctagaacag gcatgtcgat tccaatccat
     6781 gtattagtaa atttagcaga acatcctaat atcattggtt tgaaagaagc ctcaggagat
     6841 atggcttatg tgatggatgc cgctcggttg attggagaag aattcttcct ttacagtgga
     6901 aatgatgatt tgatcctacc agtaatgagt gtaggaggaa gcggtgtaat aagtgtatgg
     6961 gcgaatatcc aacctaaaat agtccatgaa ttagtaaaag atacgcagga cggaagatgg
     7021 cagcaagcaa aagaaaagca attaaatgca cttgaactga tccatgcctt gttcagtgag
     7081 accaatccta ttcctgtcaa agcagccatg tcattattag acttaccttc cggacctcta
     7141 cgcctgcctt tagtttctct ttcaaaagag aaaaagaaac aattagctca gcttttgtta
     7201 aaagaaagga cggttgaaaa atgaaaatcg gactgattgg cagtggacag atgggaaata
     7261 gagtcgaaga agttgcacaa gaaagaggag atacggtcct tcttttacgg acggcaccta
     7321 aagaagcaac tacgcttgct tttacggaac tgccagaact gcttattgat ttttctcatc
     7381 cagataattt agagaaaatc ctttcgtata gcttatcata tcaacttccg ttagtcattg
     7441 gcacgacagg ttatacacaa tcacagttcc aagaaataaa agaacatgcc aaacatgtac
     7501 ctgtgatgta tagtgctaat ttttcgttcg gcatcatggt gatgaatcaa ctaatcaagc
     7561 aagcagctgc aatgttgcaa ggctggcaga tcgaactgat tgaaaaacat catagtcgta
     7621 aaaaagatgc accatctggt actgcaaata tgctgcttca aacaatccaa gaagtgcaaa
     7681 agctgcagcc cgtatacaac tggcaaggga aaaaaagaga agaaaaccaa attggtgtac
     7741 acagtattcg tgcaggttct ttacctggtg aacatgatgt attgtttgct gctactgatg
     7801 aaatatttac gatcaaacat gaatcttttt ctaatcgaat ctttgctgca ggcgcagtag
     7861 aagcagcgga ctggttgaaa gatcagtcac caggttatta caaattagaa gatttattaa
     7921 tggacaaagg ggtgtaaaaa acgtggaagc tcaagaaatc atccgctaca ttcaaacggc
     7981 atctaaaaag acgcctgtga aagtctatct gaatgtaacc gagccaatcg aatttaaaga
     8041 aagtaaagtt tttggtgaag gaagcagctt cgttgttttt ggtgactatg agacgattgc
     8101 tccggtttta gaagctgaat cagaaaaaat cattgaaatc gaaatcgaaa ctgctgcaag
     8161 atacagcgca gttcctttgc tagatattaa aaaaattaat gctcgtatcg aaccaggtgc
     8221 tattatcaga gatcaagtgt caatcggcaa caatgccgtg atcatgatgg gagcgattat
     8281 caatattggt gcagtaatcg gtgaaaacac gatgattgat atgggtgctg tcttaggtgg
     8341 acgagcgaca gtgggcaaaa actgccatat tggtgcagga gctgtcttag caggtgtgat
     8401 cgaaccagca tcggccaaac cggttatcgt agaagacggc gtactggtag gtgcgaatgc
     8461 agtgattgtt gaaggtgtcc atatcggaaa agatgcagtc gtggcagcag gagcagtggt
     8521 tttagaagat gtggcagcag aaactgttgt cggtggaata cctgcacgtg tattaaaaat
     8581 aagtgatgat cagacaaaag aaaatacagc attgattgct gcgttacgcg aattatagag
     8641 gagatggaga aaaatgcgcg aaatagatac ctgtattgtg ggggcaaccg gattagtcgg
     8701 ccaaacgatg ctcaaagtgt tagaagaata tgattttcca gtaggacgat tgaaattatt
     8761 agcttcaaaa cgttcagccg gtaaagtttg tgtattcaag aaaaagaaac acactgtaca
     8821 ggaattgaca gaaacttcat ttgaaggata tgatttagcg ctattttctg caggtgcttc
     8881 tgtatccaaa gagtttgcgc caatcgcacg aagtcaggga ctatttgtca tagataattc
     8941 ctcagcatgg cgggaagatt cggatattcc attaatcgtt ccagaggtca atctggaaga
     9001 ctatacattg aatcggctga ttgcaaatcc aaattgttca accattcaag cggtacttcc
     9061 gcttaaagct ttacaagaag cctttggtat ccagcgagtc aattattcga cctatcaagc
     9121 agtttctggt tccgggcaaa aaggcattga agatcttcat cgtacgcaaa atgacgagga
     9181 accagttttg tatccttata atattagcca aacggtgatt ccggaaatcg atcagccttt
     9241 ggaaaatggg tatacaaaag aagaacagaa aatgataaat gagacccgta agattctgca
     9301 tcagcctgct ttgccagtat cagcaacctg cgtacgtgtg ccaatcgaaa atggacatgg
     9361 agtctctgtg gcagttcaac tgcaaaaaga gtttacgacg gaagaaatta gagagtgtct
     9421 cagccagttt ccagggattc aattagaaga tgatctagca gcacataaat atccgacgag
     9481 cattcttgct agaggaacag atatggttta tgttgggagg atcagaaaag acacttcttt
     9541 agagaacgga ttgttgttct atacgactgc cgataacata cgcaaaggag ccgctgcgaa
     9601 tgccgtacag attgctgcag ctttaatgca gaaaatagcg gaggtggcaa gatgagtaat
     9661 acgctgaaag tagcgaaatt cggcggaaca tcgatgtctc atgaagagca attcaaaaaa
     9721 gtccgtacaa ttgttctttc agatcccaat cgccaagtga tcgttgtttc tgctttaggc
     9781 aaacgtacgt cagatgatga caaagtaaca gacttacttt atttgattta tgcttacctt
     9841 cagcatcatg ttgattggac acccctttgg aaaaggattt gtgatcgttt cattcaagta
     9901 agagatgttc ttgccctgca gttacctatt gaagatgaac tagcacgcat acagaaacaa
     9961 ctggaagacg aaaagataac tgaagattat ttagtaagta gaggtgaata tctgactgct
    10021 caactgatgg ctgagtatct tggttaccag tttattgacg caaaagacct gattttcttt
    10081 gactatgatg ggaaaatcga tcaggaaaaa acagcacagg aaatcacaaa agcatttaaa
    10141 agatatccga aaatggttgt tccagggttt tacggggcga accctgcagg aaaagaaaaa
    10201 ttattgggcc gtgggggaag cgacattacc ggcgcgatac tggcgaagat gttgcaagcg
    10261 gaaagttatg agaattggac ggatgtttca ggaatcatga tggctgatcc gcgaatcatc
    10321 gatcatccta aaatcatcga ggaaatctct tttagagaac tgagagagat ggcttatatg
    10381 ggggctaacg ttcttcatga agcagctgtt ttcccagttc aagaggctga tatccccatc
    10441 caaataaaaa ataccaatga tcctttcgct caagggacaa aaatatcgaa tcacgaaata
    10501 gaaaaggaaa atggactgac aggaatagct ggacgcaagg attttctttc catcacttta
    10561 ttcaaacgtc atatgtccga tgaaatcggg tttatctgga aagcaatgag catttttgct
    10621 aaacatgaga tatcaatcga acatattcct tcagggatcg acaatatcgg agtgatcgtc
    10681 tcagcagaag cgattgccga taagttattt ctcatcacga aagaattaaa agaaacactt
    10741 ggcgtggaag aaatcgtcgt gatcgaagac ttagctttga tctctgtcgt tgggggtccg
    10801 cataaagaat tgattggatt atctgggaaa gtgttatcga tattgaacaa attagaaatt
    10861 cgcacatcga tcttatcaca aggagcacag gagttgaatc tgatcatcgg tgtaccgaat
    10921 aatcaatatg aaacagtagt gaagggtatc tatgaaggga tggtgaatac ggatgctgaa
    10981 agaatatcga atggcactcc atcaaattcc tgaaatcggg tttcatgaat tcaagacaaa
    11041 aaaatattta tatgaacaag taaaaaacag cgaggccgtt atccatgaaa tcggcgaaac
    11101 aggactactc ctttattttg ataatcagca agaagaaacg attgcttttc gaacagatat
    11161 cgatgcactg ccagtcaaag aagaaacgaa tctctctttt gcttctaagc atgaaggtta
    11221 tatgcatgca tgtggtcatg acggacatat ggcgatgctt ttagggttga ctgactttat
    11281 aactaaacac attgaggatc ttatttataa tgtagtactg atctatcagc catcagaaga
    11341 aatttccggt ggcgcacaaa gcgttattca gtcaggattg ttggataaat atgaagtaca
    11401 agcaatattt ggctttcatc tatggcctgg actagctaag ggagaagttt tctctcgacc
    11461 aggacctttg atggcacaaa gcagtgaaac agacattatc attaaaggcc agtctgcaca
    11521 tattgcttct agtgaaaaag gaatcgatag tctggaggca gctgtacgat ttttgaagaa
    11581 tgtttatgat tttgaccggc aattaccggc agagtggaat catctaatga aatttggcca
    11641 aatccaaggt ggaacgatcc gaaatgtttt agctggagaa gttattgtat ccggaagtat
    11701 ccgttcttac agttcagata tgcaaaatta tttgaagagg ggattagacc atttagcaga
    11761 acagtttcac tctacagcta ctatttcttt tcgctataat gacggctatc cagctgtaat
    11821 caaccataga ggcttatatg cagcactgtt agattcaaac aaactacatg aattagatgc
    11881 tccagttctt caagccgaag attttggcat atatacgcaa caatatccat gtgtattttt
    11941 ctttttaggt gtcggtgata cccctgcttt acatgatccg acgttcgatt ttgatatgga
    12001 tgttttagaa aatggtgtag aatggtacaa aacgattctt tttacagaaa atcttcttgt
    12061 aaaaatcgat agcagttcaa aagaggagcg agtaaataat gatgaattta tccaatcgat
    12121 ttagtcctag gctttctaga atagaagtat caaaaatacg acaattcgat cagcaaatat
    12181 cgtcgatacc agatgtgatc aaactcaccc tgggagaacc ggattttcca actcctgaac
    12241 atgtcaagca agcaggaata gcagcgattg aagaagactt ttctcactat accggaatga
    12301 gaggattaga ggaactgcgg gaagcggctt gtatatttca gcaacagcgt tatggattaa
    12361 cttatgatcc gcaaacggaa gttttgacga cagttggtgc aacggaagcg attgcctctg
    12421 cgttgttatc tgttttggaa gaaggagaca aggttcttat tccagcgcct gcttattcag
    12481 gatatcagcc attagtggaa ctagctggtg ctgaattgat cccgatagat acttctgaca
    12541 cagggtttgt ctgtcagccg gaacaatttg aacgagcatt tgaacaatat ggatcagctg
    12601 tcaaagcagt cattttaaac tatcctaaca atcctactgg gacgacattg tctgcaaaac
    12661 aattgaaagc aatcgctgag gttttaaaaa aatatccggt atttgttata agtgatgaag
    12721 tatatgctga attgacttat agtggaacgc atatgtccat agctacttat cttccagaac
    12781 aaacaatcgt gataagcggg ttatccaagt cacatgcgat gactggttgg cgagtgggct
    12841 ttatttttgc tcaaaaagcc cttattgacg aactaattaa agtccatcaa tatctggtga
    12901 ctgccgctac aacgatgtcg caaaaagctg cggctgaagc gctaataaat ggggtgaaag
    12961 atagcgagaa tatgaaagag caatatcaaa cacgaagaga ctatctggtc cagcaattgg
    13021 cgcctttagg atttgaagtg acccagccaa acggtgcatt ttacttgttt tgcaaactcc
    13081 cagctgccat tcaaactgac tcttggcagt tttgtgtaga attagcggaa caggcaaaag
    13141 ttgcgtgtat cccaggaatt gcatttggcc cagaaggaga aggttatatc cggatttctt
    13201 atgctagcag catggaaaaa ttacatgagg cctgcaaacg gatcaaagac tttttaacag
    13261 aacagtgatt catagatttt atcaaaaaaa caatcgttaa ttttgcggta tattctgcaa
    13321 ggagacgatt gttttttaaa tttagttcaa atgaacgcgt ttcttttgat tcataactaa
    13381 tgtaatgtta agtttaaatt gtttagaatg attttaaaag aatattcaag gaggtttcat
    13441 caatggaaaa aatcaaagtt gtcattgtag gtgcatctca tggagggcat caatcgatct
    13501 tggaactatt gacacgatac ggtgaaaatg tagatatcac gctatttgaa gcaggagact
    13561 atatttcctt catgtcatgt ggtatggagc tgtatttaga agacaaggta acagatgtaa
    13621 atgatgtacg taatttcaga ccagaaaact ttccgcaatc caatgttcat attttaaata
    13681 atcacgaagt aaaaacgatc gatgccgata aaaaaacagt aacggttact agcgcatcag
    13741 ataacgaaac aaaagaatat ccttatgaca aattgatttt gagttctggt gtcaaaccaa
    13801 acaatctgcc agtaccagga gcagatctag aaaatgtcta cttgatgcgt ggatacaatt
    13861 gggcaaccaa aatcaaagaa aaattaaatg atccggctat taaaaaaatc gctgtcgtag
    13921 gtgcgggtta cattgggatc gaagcagcag aagcaagccg aaaagctggg aaagaagtcg
    13981 tattgcttga tgtgatcgat agacctttag gcacttattt agatgctgag atgacagata
    14041 ttttagaaca acacctaaat gaaaacggta ttgaagtttt gacaaatgca aaaatcgaag
    14101 cttttactgg taacggaaaa gttgaggcaa tcaaaacaag tgaaaaagaa atcccagcag
    14161 atctagtcat tcaagctgcc ggtgtgaagg caaatacaga atggttaaaa gggatcgtcg
    14221 acttagatga acgtggctgg atccaaacaa atgaatactt gcaaacgaat cttccagatg
    14281 tgtatgcagt aggagatgct acacttgctt attctatccc agcacgcaaa aaaatgccga
    14341 tcgctttagc gactgttgca cgtagagaag cacgttatgt agtaaaacat ctttttgaag
    14401 aagtaccaaa caaaccgttc ggcggagtcg taggttcctc tgcattaagt gtctttgact
    14461 atcattttgc agcaagcgga ttgaatagct ttacagcagc aaaagcagga gtagatgtac
    14521 gagtgtctta ttatgaagat acgttacgac caaaatatgt accagaagaa caaggaaatc
    14581 caaaagtgtc catccaatta ttctcggatc caatgaccca tcaattgcta ggaggagcag
    14641 tattatctac ctatgatgtt acaggtcaag gaaatgtatt ggcactagcg atccaaaaca
    14701 aactgacttt agaagaacta gcagaagctg acttcttctt ccagccaggc tttgaccgac
    14761 aatggagcat gttgaatcta gccgctcaac aagcgttagg agaagaacca tttgtagaat
    14821 aagaaaacga aatatttgaa aagaggttgt gaaaaagctg ccttgcatca agtaataaga
    14881 acagccaagt aaaaacagct tcttatgttt ttaacttggc tggacttatt accgcagatg
    14941 caagcttttg aacaccgttt attttcaaaa aagggactgt gacacgattt gtgtaccgac
    15001 ccccaaaagt tagaccaaaa aatctaactt ttggaggtcg gtatttttta tggttaaata
    15061 tacttttgaa cttaaattaa agatcgttca tgattatttg gatggcaaag gcgattggac
    15121 cgtaaaaatc cagatcaaga gattgaagcg gaaatgctta aaattcggga gaagcacaag
    15181 gattatggct gtttacggat gaccaatgaa ctaagaaacc gcggtttctc tatcaataaa
    15241 aagaaagttc agcgcttgat caagaagcta ggaataaaag taacggcata cacgcgtaaa
    15301 tcacgtcgct acaattctta tc
//
LOCUS       DASJSZ010000064        15206 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.065, whole genome shotgun sequence.
ACCESSION   DASJSZ010000064 DASJSZ010000000
VERSION     DASJSZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.065"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(93..1136)
                     /locus_tag="U4B03_001974"
     CDS             complement(93..1136)
                     /locus_tag="U4B03_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEN2068816.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1513..2478)
                     /locus_tag="U4B03_001975"
     CDS             complement(1513..2478)
                     /locus_tag="U4B03_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEN2068817.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2712..3557
                     /locus_tag="U4B03_001976"
     CDS             2712..3557
                     /locus_tag="U4B03_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2068818.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3748..4935
                     /locus_tag="U4B03_001977"
     CDS             3748..4935
                     /locus_tag="U4B03_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN2068819.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4941..5297
                     /locus_tag="U4B03_001978"
     CDS             4941..5297
                     /locus_tag="U4B03_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEN2068820.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5299..5637
                     /locus_tag="U4B03_001979"
     CDS             5299..5637
                     /locus_tag="U4B03_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEN2068821.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5713..5969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6032..7066
                     /locus_tag="U4B03_001980"
     CDS             6032..7066
                     /locus_tag="U4B03_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2068822.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7059..7745
                     /locus_tag="U4B03_001981"
     CDS             7059..7745
                     /locus_tag="U4B03_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068823.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7769..8617
                     /locus_tag="U4B03_001982"
     CDS             7769..8617
                     /locus_tag="U4B03_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN2068824.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8814..9587
                     /gene="sufC"
                     /locus_tag="U4B03_001983"
     CDS             8814..9587
                     /gene="sufC"
                     /locus_tag="U4B03_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEN2068825.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9600..10886
                     /gene="sufD"
                     /locus_tag="U4B03_001984"
     CDS             9600..10886
                     /gene="sufD"
                     /locus_tag="U4B03_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEN2068826.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10883..12118
                     /locus_tag="U4B03_001985"
     CDS             10883..12118
                     /locus_tag="U4B03_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2068827.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12105..12575
                     /locus_tag="U4B03_001986"
     CDS             12105..12575
                     /locus_tag="U4B03_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEN2068828.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12580..13971
                     /gene="sufB"
                     /locus_tag="U4B03_001987"
     CDS             12580..13971
                     /gene="sufB"
                     /locus_tag="U4B03_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEN2068829.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14069..>15206)
                     /locus_tag="U4B03_001988"
     CDS             complement(14069..>15206)
                     /locus_tag="U4B03_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2068830.1"
                     /translation="TFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAEAQ
                     TALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILPAF
                     GELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTLLP
                     RKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWEDID
                     YFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMILY
                     GYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLFES
                     GASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4973 a         2836 c         3250 g         4147 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccagtac aaaaacatga
     1441 acagctgttt ttgtttggcc gttcttatta cttgatgcag aacagctttt tcacaaccta
     1501 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
     1561 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
     1621 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
     1681 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
     1741 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
     1801 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
     1861 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
     1921 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
     1981 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
     2041 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
     2101 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
     2161 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
     2221 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
     2281 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
     2341 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
     2401 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaataat
     2461 tttcaaaaag ttcttcatat ataatattct cctttttatt taatagattt tagtaccaag
     2521 atgaacgaat acattgtaaa atgaatagga atgtatgatt acgagcaagc agacagctga
     2581 ttgtcttcat ctaagttact agttggatca gcaactgttt tgttgtacat ttctattata
     2641 gatggttttt tgtatattta aatcaaatag tttttatagt aattatcata aaaagttatg
     2701 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
     2761 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
     2821 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
     2881 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
     2941 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
     3001 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
     3061 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
     3121 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
     3181 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
     3241 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
     3301 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
     3361 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
     3421 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
     3481 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
     3541 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
     3601 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
     3661 tcccaaatgt ctatggaaag actttccatt atctaactgg gccgctataa taatcagcag
     3721 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     3781 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     3841 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     3901 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     3961 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     4021 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     4081 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     4141 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     4201 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     4261 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     4321 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     4381 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     4441 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     4501 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     4561 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     4621 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     4681 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     4741 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     4801 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     4861 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     4921 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     4981 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     5041 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     5101 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     5161 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     5221 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     5281 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     5341 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     5401 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     5461 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     5521 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     5581 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     5641 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     5701 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     5761 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     5821 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     5881 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     5941 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     6001 tgaaaaatta taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     6061 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     6121 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     6181 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     6241 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     6301 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     6361 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     6421 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     6481 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     6541 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     6601 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     6661 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     6721 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     6781 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     6841 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     6901 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     6961 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     7021 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     7081 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     7141 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     7201 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     7261 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     7321 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     7381 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     7441 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     7501 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     7561 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     7621 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     7681 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     7741 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     7801 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     7861 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     7921 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     7981 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     8041 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     8101 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     8161 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     8221 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     8281 aacattcgat gatattgaag aaaaacctaa gaacttgaag ttcaaccata cgatcgaccc
     8341 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     8401 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     8461 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     8521 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     8581 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     8641 aataagtctg taatggctta tttctcatct tttttatacc tattctaatt tagaaatcaa
     8701 aattgtttaa taataaataa aagatatttg attttagtaa ggaaggtcat tgcgcttcat
     8761 tctcaattag tctaaaatga gtgagaacga aagagaattt gggggtataa tgcatgtctg
     8821 tcttagaaat aaaaaatcta catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa
     8881 acctaacaat gaaaaccgga gaaatccatg ccatcatggg acctaacggc acaggaaaat
     8941 ctaccttatc tgccgctatc atgggcaatc caaactatga agtaacagaa ggcgaaattt
     9001 tgtttgacgg gaaaaatgtt ttagacttgg aagtagatga acgtgcgcga cttggcttat
     9061 ttttagctat gcaatatcca agtgagatac caggtatcac gaatgcggaa ttcatgcgtg
     9121 cagcgatcaa tgcgaaacgt gatgaaaacg acaaaatttc tgttatggca tttttgaaaa
     9181 agttagatga aaaaatggct ttactaaata tgccagaaga aatggctgaa cgttatctga
     9241 acgaaggttt ttctggcgga gaaaagaaac ggaatgagat cctgcaattg ttgatgttag
     9301 aacctacatt tgcaatttta gacgaaatcg attcaggtct ggatatcgat gctttgaaag
     9361 tcgttgctaa aggagtaaat gaaatgcgcg gagacaattt cggtgcattg atcatcaccc
     9421 actatcaacg attattgaac tatatcacac ctgatgtcgt tcacatcatg atggaaggcc
     9481 gagtagtcat gacagggaat gctgacctag ctaaacgtct ggaagcagaa ggctacgctg
     9541 gtatcagtaa agaattagga atcgactata aagaagaaga agcgtaaggg ggagcaagaa
     9601 tgaaagaaaa aaactggctc gattatcttg acgcggtaaa tgatttttct ttaagtaaag
     9661 gcgaacctga ttggatgaga accttccgtc aagacgcact agcgaaagca gatgaactgc
     9721 cattgccgca tatcgatcgg gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa
     9781 cacaaaccat cagtgaaaca ggcacgattc cggcatttga tgcaatgaaa gacaatcctg
     9841 tgcttgtcca acaaggttca tggacgattt ttgaacaact gcctgtggaa ttagcagaaa
     9901 aaggagtcat ttttaccgac ttgttcactg caatgatcga atatccagaa ctagtccaag
     9961 aatattatat gaaaaaagca gtaaacatga acgaagacca attgactgca ctccatgtag
    10021 cttttatgaa cagcggtatt ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac
    10081 tcgagtcctt attcattcaa gatggcgcat ccgatgagca cttcttcaag catgtgttga
    10141 tcgtggcaga tgaacatagt gaattttctt atctggaacg tttccagaca accaaagaac
    10201 aagtagcaaa atcatcaggt aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa
    10261 tcaaatattc agccgtagat caattaggag aaaatattac ttcttatatg aatcgccgtg
    10321 ggcacatttt acgagatgct tctgtcgact gggcaattgg tgtcatgaat gacggtcatg
    10381 tgatcgctga ctttgactca gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg
    10441 tggcaatcag ctcgggcaga caaatccaag gaatcgatac acgagtaacc aataaagccc
    10501 ctcatacgat cgggcatatt ttgcaacacg gagtgatccg agaaaaagga acattgacct
    10561 ttaatggtat cggccacatc ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc
    10621 gtgtcttgat gctttctgat aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg
    10681 aaaacgaagt aactgctgga catgctgcca gtgtaggacg agttgatcca gaagaaatgt
    10741 actatctgat gagtagagga ttgcataaag aagaagcaga gcgcttagtg attcgaggat
    10801 tcctcggttc tgtcttaaca gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag
    10861 taatcgaagg gaagttgaat ggatgatcga tgctaaaaag ctacgcaatg attttcctat
    10921 cttatttcaa gacgtcaatg atgaaccttt agtttatctg gataatgcag cgacgaccca
    10981 aaagccaaaa caagtactcc aggcattagc gtactattac gaacacgata acgccaatgt
    11041 tcatcgcggt gtccatacgt tagcagaacg cgcgacccat gattatgaac aagcaagaga
    11101 aaaagtacgg gcgttcatcc atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac
    11161 gacgacaagc ttgaattggg tagccagaac cttcggcgac cgctatatca aagcaggaga
    11221 cgagatagtg ctctcttaca tggaacatca ttctaatgtt atcccgtggc agcagttggc
    11281 aaaacgaaca ggtgctgtct tgaaatatat cgaactaaca gaagaaggct tcctggatat
    11341 ggaggatgca gcgaaaaaga ttacggacaa aaccaaaatc gtctccattg ctcatgtatc
    11401 caatgtctta ggtgtcttca atcctgttca agaattagct gaacttgtcc atgaaaacgg
    11461 cggggtcatc gtagtagatg gtgctcaatc tgccccgcac atggccattg atgtccaagc
    11521 actagactgt gatttctttg ctttcagcgg acacaaaatg tgcggaccaa caggtattgg
    11581 tgttttatat ggtaaacgaa aatggttaga agaaatggaa cctgttgaat ttggtggcga
    11641 aatgatcgat tttgtcgaat tatacgacag tacatggaaa gaactacctt ggaaattcga
    11701 agcagggaca ccaaatatcg ccggcgctat cgcattaggc catgcaatcg attatttgca
    11761 aaaactcggt atggacaata tccatcgata tgaagaagaa ctagccgctt atgtgttgcc
    11821 aaaattacaa gcaattgatg gattgactgt ttatggtcca caagacccga attttcggac
    11881 aggagtcgtt gcattcaatt tggatgggtt acatccccat gatgtcgcga cagctttaga
    11941 catggaaggg gtagccgtca gagcaggtca tcattgtgcg caaccgctat tgaagtacct
    12001 tgaagtatca gcgactgctc gcgcgagctt ttatttctac aacacaacgc aagacgccga
    12061 ccgtttagtt gaagcaatca tggcaacaaa ggagtttttc caacatggga ctatctaaat
    12121 tagacaattt ataccgtcaa gtgatcttgg atcattcttc ccatccgcat catcgtggaa
    12181 gcttgactga ctcgacaagc aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg
    12241 aattgcaagt cgatgtaaaa gacggaatca tcaaaaatat cgcctttgac ggcagtggct
    12301 gctccatcag tacggcaagt gcctcgatga tgacagacgc cgtcatcgga aaatcaatag
    12361 aagaagcaga acatttaact gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag
    12421 atcccgacca attaggcgat gctgctatgc ttggtggtgt agctaaattt cccgctcgga
    12481 tcaaatgtgc gacacttgcc tggaaagcac taggaaaagc aatggaaaac gatggtaccg
    12541 cagctttcca tcattaccac aaggaagagg agtgagaaca tgggcgtacc agaattagaa
    12601 gaatataaat tcgggtttca cgatgatgtc gaaccggttt tcagtacagg agaaggcttg
    12661 acagaagaag tgatccgtga gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa
    12721 ttccgtctga aatctctaga aacatttaac aaaatgccaa tgcaaacatg gggccccgac
    12781 ctttcagaca tcgatttcga tgccatcaaa tattaccaaa aaccaagtga caaacccgct
    12841 cgtgactggg aagacgtacc agaaaaaatc aaagatacat ttgaaaagat tggtatacca
    12901 gaagctgaac gagcttatct agccggtgct tccgcacaat atgaatcaga agtcgtgtac
    12961 cataatatga aagatgaatt cgaaaagtta ggaatcgtct ttactgacac tgactctgct
    13021 ttgaaagaat accctgaatt attcaaagag tacttttcaa aacttgtacc gccaaccgac
    13081 aacaaattag cagcgctgaa ttcagccgta tggtcaggtg ggacctttat ttacgtgcca
    13141 aaaggcgtca aagtcgatgt tccattacaa acgtacttcc ggatcaatgc ggaaaacacg
    13201 gggcaattcg aacgtacgct gatcattgtt gacgaaggag caagcatcca ctatgtcgaa
    13261 ggttgtacgg cacctactta ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt
    13321 acaagaaaag atgcgtattg ccgttacaca acgatccaaa actggtcaga caatgtgtac
    13381 aacttagtaa ccaaacgagc aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga
    13441 aacctaggtg caaaaacaac gatgaaatac ccaagtgttt acttagatgg aaaaggtgca
    13501 cgaggcacca tgctctccat cgcctttgca ggagcaaacc aaatccaaga tacgggtgca
    13561 aaaatgatcc acaatgcacc aaatacatct agctctatcg tctctaaatc aatttcaaaa
    13621 gacggcggag aagtaaacta tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg
    13681 atctctcata tcgaatgtga cacgatcatc atggacgaat ggtcgaaatc cgatacgatc
    13741 ccattcaatg agatccacaa cagccaagtc tctttagaac acgaagccaa agtctcaaaa
    13801 atctcagaag aacaactcta ctatctgatg agccgaggcc tgaccgaatc tgaagcaact
    13861 gaaatgatcg tcatgggctt tgttgaacca ttcaccaaag aacttccaat ggaatatgca
    13921 gtcgaactca atcgattgat cagttatgag atggaagggt ctgtaggtta ataactattt
    13981 cattgctcga aacgttgata ttaaagcgtt tcgagcgttt ttttatttgt attttaatgg
    14041 ttggctacgt ttttggctac gtttggaatt attcgcccat aaattttgca aatcggtcac
    14101 cagttttctt aacagattgt ggtgtgacat gagcatagat attcatggta gttttaatat
    14161 ctttatgacc taatcgttcc tgtacttctt ttatcgacgc accactttca aacaataagc
    14221 tacagtgggt atgcctaaat ccatgtggag taatttgagg ctccatcggt gtctttttgt
    14281 aaatccagtc taaccaatcg tttactactt gggggtagta caacttgttt gtgtttgtag
    14341 tgaaaagaaa ctgttttggt gaattcgtgt tatagccata taagatcatt attttcattt
    14401 gttcttgttg ccaaatacgc agttgtttaa gtgtaaaatc atcaagctga atcgttcttg
    14461 atgaattttt agttttaggt acttgtagaa ctacttgatt aaactcgtct tgagcgatag
    14521 ttttaccaat tgttattgtt ttattgaaat agtctatatc ttcccattga agcgccaatg
    14581 cttcgctttt acgcattcct gtataagcta ataaacggaa aaaagtgtac agtttaactg
    14641 aattattttc tttagtattc tgtaaaaaca gttgtaattg atctttcgtg taaaacttca
    14701 agggttgttc ttcttcaatt tttcttggaa gcaatgtttt agacatagga ttatccttac
    14761 agacttccat tgctactcca aatttaaata tttgagcagt tgtttttctc aagaaaccat
    14821 attgttttaa tgttttgtgc cattcattca cctgtttttg gcaatagcta acacttattt
    14881 tatccaatcg taattcccca aaagcaggta atatatggtt tttgcaaaaa cgttgagcaa
    14941 cggcaacaga agacggtttt actcttgttc tgtagttatc aatccataat tcatacaact
    15001 cttgaaaagt tgtaacattg attttaaact cttgtttttc tttaaagtct acaagtagtc
    15061 tattaagtgc tgtttgcgcc tcagcttttg atttgaatct tctacgcttt gtacgtatat
    15121 cttttccagt cttgggatct ttccctaaat aagtttcaaa tccccaatat ttttcgccat
    15181 tttgtaattt ataactaaaa aatgtt
//
LOCUS       DASJSZ010000065        14601 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.066, whole genome shotgun sequence.
ACCESSION   DASJSZ010000065 DASJSZ010000000
VERSION     DASJSZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.066"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..478
                     /locus_tag="U4B03_001989"
     CDS             <1..478
                     /locus_tag="U4B03_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068831.1"
                     /translation="SSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLL
                     LKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVL
                     KQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(745..1914)
                     /locus_tag="U4B03_001990"
     CDS             complement(745..1914)
                     /locus_tag="U4B03_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN2068832.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            2118..3293
                     /gene="mltG"
                     /locus_tag="U4B03_001991"
     CDS             2118..3293
                     /gene="mltG"
                     /locus_tag="U4B03_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEN2068833.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            3429..3908
                     /gene="greA"
                     /locus_tag="U4B03_001992"
     CDS             3429..3908
                     /gene="greA"
                     /locus_tag="U4B03_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEN2068834.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            4077..4808
                     /locus_tag="U4B03_001993"
     CDS             4077..4808
                     /locus_tag="U4B03_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEN2068835.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4805..5872
                     /locus_tag="U4B03_001994"
     CDS             4805..5872
                     /locus_tag="U4B03_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2068836.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5879..6511
                     /locus_tag="U4B03_001995"
     CDS             5879..6511
                     /locus_tag="U4B03_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2068837.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6651..7316
                     /locus_tag="U4B03_001996"
     CDS             6651..7316
                     /locus_tag="U4B03_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEN2068838.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            7344..7637
                     /locus_tag="U4B03_001997"
     CDS             7344..7637
                     /locus_tag="U4B03_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEN2068839.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(7705..8652)
                     /locus_tag="U4B03_001998"
     CDS             complement(7705..8652)
                     /locus_tag="U4B03_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEN2068840.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8830..9633)
                     /locus_tag="U4B03_001999"
     CDS             complement(8830..9633)
                     /locus_tag="U4B03_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEN2068841.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9779..10693
                     /locus_tag="U4B03_002000"
     CDS             9779..10693
                     /locus_tag="U4B03_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2068842.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10741..11211
                     /locus_tag="U4B03_002001"
     CDS             10741..11211
                     /locus_tag="U4B03_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEN2068843.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            11296..11904
                     /locus_tag="U4B03_002002"
     CDS             11296..11904
                     /locus_tag="U4B03_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEN2068844.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            12038..12238
                     /locus_tag="U4B03_002003"
     CDS             12038..12238
                     /locus_tag="U4B03_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2068845.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            12992..14218
                     /locus_tag="U4B03_002004"
     CDS             12992..14218
                     /locus_tag="U4B03_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2068846.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(14326..>14601)
                     /locus_tag="U4B03_002005"
     CDS             complement(14326..>14601)
                     /locus_tag="U4B03_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN2068847.1"
                     /translation="LLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRNIFIAAID
                     ITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         5005 a         2216 c         2910 g         4470 t
ORIGIN      
        1 ttcatcagta gttaacatgg tgaaagaaat gaattttcaa ggaacttatt ctaatgaaca
       61 aatggaaaat aaacttgaag aatttgagaa ttttgattat gtacctatag gctataaaaa
      121 agatcaattg cttttgaaaa accatcaagt tacagaagat ggtcaagtta tttatagtag
      181 cttttctcca aataaagatg aaattacatt tacgtttaaa tcttcaaaag gcaaaacaat
      241 agttttacct gtattccggt ataaaggatt aacggttact gttaattcaa aaaatgctac
      301 agtactaaaa caaggaggtc cttttgttac agtaaaaagt gaaagtggaa taaataaagt
      361 aacaatcagg tattcataca catttttttc taaaatctca ttatttattt ctataatcac
      421 gctaattgtt cttctttttt attgcataaa attaaaaata agaacaagcc atgaatgatt
      481 tgctagcaca tattttagca atctaaagat actattttag atttaatagg ccctttgtca
      541 aataatgttg gtaaagagaa atcttagaat aatttggaca gaacagaaag aagaacacct
      601 ctttctgttc tgtttatttg gaattaaagt ctgtagcaaa aattgatttt tagatttgtc
      661 ccacactctt tcctacgttt agtcgagtac tttttttaag caaatgctat tcaaagaagt
      721 agtatttgct atcttttcat catatcagct atttgccaac ttagtagttc cttccatcag
      781 gtcatagatt tggtttctta gatcagcagt aggcagtttg atagttttgt cagcttttat
      841 attgctttcc atgatgatag catcctgtga atctttgcta aatatagcag atgtatcaaa
      901 agagtcgata tttccttgcg acaggatata attatctccg ctatataccc caccactata
      961 tggatgtcca ttcgcatttt cacgccataa tgagaagagt aagtctttgg aaactagttt
     1021 tccagataaa acttgtgtaa acagcgtatt taaatcatag acggacatac tgatattacc
     1081 agtccctaat tcgttatgga tctctgtttc agattctgtc agtggtttaa agtaatcatc
     1141 ctctttagac atttgatagg atagtgcatg attggatgaa tttttgactt ggtcataaaa
     1201 ttttgtatgc tgtaatccta aaggttcgat gatatcatcg gtcaataatt tttcatacgg
     1261 ttgatttgtt aatttgatca aaattccagt caatatggta aagttcaatg aatcataatg
     1321 agaggtctga taatctgtaa attttaagtg ctgaatcgca aattgaacga cttcttcatc
     1381 agtcatcggt atggttggag aagcagttcg gtgcaagcct gagcgcatat acagcaagtc
     1441 acggatagta atttgctggc tgccagcaat ttgtggatag aattgagcca gcttagtatc
     1501 taaagaaagg tgcccacgct cgacttgttt taaaatcaaa agagtagtca atgctttttg
     1561 aaccgaacca atctgataat aaagagaagg atcatttggt tgttgcgtga gtttattcgc
     1621 atagccatag cctttttcca tgatgatgcg attatttttc tttacgtaaa ttgagccaat
     1681 ataatcattt tttcttaata gttgatcgat tttttcaacg agctgttgtt cattggtagt
     1741 cagtatcaac tttttttgag ctgattgagc atgggcaact atctgagagt gttcattatt
     1801 gtaaaattgc catccaagaa tagttcctgt agagaacgca ctaaggctaa acaacacgac
     1861 aacgatcatt aaaaatgaat attttctttt tttagcatgt tttccttttt tcataatagt
     1921 ttatttagtc tcctttcttt tttacagaaa gaataaagct aaaatagctt gtatcttgat
     1981 aacatttatt ttaatcttat agtaatggat ttactactta catttttgat agaatattta
     2041 aataaaatca ttcacgccaa ttcaaatatc atgtataatg aataaagtta cgaacttgat
     2101 agtaaggagg agttgttttg tcaggaaaca aacaacaccc aagtaaagat aaaaaagaag
     2161 ccagtaaggc agctacagca gatataatcg acaagcggag aaatctgcgt aaaaaagaag
     2221 ataagattgt gaggaagatc attttagtga ttgctcttac attattgatt attggcggat
     2281 ttcttggatt taccgtgtat cgttatgtag atagcgggtt gaagccttta gataaatcag
     2341 atgaccaatt agtccaagtc gagatcccga gtggctcttc taacaagcag attggtgaga
     2401 ttttggagaa ggacaatatt attaaaagcg ggattgtttt caattattat acaaaattta
     2461 aaaatttaac tggctttcaa gctggctatt atcagttagc gcctaatatg actcttgacg
     2521 agattggcaa acagttgcaa gaaggcggta cttctgagcc aacaaaagtt gcagatggga
     2581 aaattgccat tccagaaggg tacgatatcg atcagattgc tgaacgggta gcgaaggtaa
     2641 ctggtaaaga caagaaagaa tttttagatt tagtgaatga tgagactttc tttaacgagt
     2701 tgcaccaaaa attcccagaa ctgttggaaa gtgcatctaa agctgagaac acaaaatacc
     2761 gtttggaagg ttacttattc ccagccacat atgattatta cacgagtact tcgcttaagg
     2821 atctagtgat tgaaatggtg aacaagacga atacggtcat gcaaaactat tattcagcta
     2881 tcaagcagaa aaatttgact gtacaagaag ttttaactct agcttctcta gttgaaaaag
     2941 aaggcgtaaa agaaaatgat cgtaaaaaca ttgcacaagt cttctttaac cgaatcaaag
     3001 caaatatgcc acttcagtca gatatctctg ttttatacgc attaggtgaa cacaaagaat
     3061 tagtgacgta tgaagataca gctgtagatt caccatataa tttgtatacc aataccggct
     3121 atggacctgg accttttgac aatccaagcg aagaggcaat caaggcagta ttagaaccag
     3181 cagaaaatga ctattattat ttcgtagcag atacgagcac tggaaatgtt tatttcgcta
     3241 aaacttatga agaacatatg gaattagtgg aaaaatatgt aaataattct tagaaaaaca
     3301 ggctctagac attgatcatt tttagaaaaa catttacatt tgaagaaaag cgtggtaatc
     3361 ttttttggac tatattcctc aaaagattac cttttgcggt attttatcga aaaaaggaga
     3421 cgttacacat ggtagagaaa gtttatccaa tgactctaga aggtaaagaa aaattagaaa
     3481 aagaattaga agaactaaaa acagtcaaac gcggagaaat cattgaacga atcaaaattg
     3541 cccgcggttt cggagatctt tccgaaaact ctgaatatga atcagcgaaa gatgaacaag
     3601 catttgttga aggaagaatc acaacaattg aaaatatgat tcgttttgct caaatcatcg
     3661 ataacgacgg tgtagatgca gatgaggtat caattggtaa aacagttaca ttcatcgaac
     3721 taccagatgg agaagaagaa gagtacacga ttgtcggaag cgctgaagca gatccgtttt
     3781 ctggcaaaat ttccaatgat tctcctattg cgcaagcatt gattggcaaa cgtaaaaatg
     3841 atgaagtaac tattgctact ccaggtggcg atatgttagt taaaatcgtc aaagtagaac
     3901 aagcgtagtc tgactgaaag atctagagag ttcatcttac tgatgataca ctctagatct
     3961 ttttctcttt gtgtatttta gatttcaaca aacgatctta catctaaagt cctatagcca
     4021 acagttaaaa aaataggtat gatgaaatga gaatgtgtca ggagggagaa agagcgatga
     4081 atagttcttg gcgttttttt gtggtagttg aagcactact gctgttattt gctgtttggc
     4141 aaatcgtaaa caatactgga cttttgcttt tagtgatatt cggtattttc aatatatatc
     4201 tagctatgcg caaacatcct cgtactaaat ttcaaaattt tcaattgata ttaggaagtt
     4261 tggtcatttt ttttagctta gtcaacagtc ctgctttatg gatgatggct gtatttgccg
     4321 ttttatttat tggtctgaaa ggtgtagaga tttcagggat agatttaacc aaaaatgctt
     4381 tttggcgaaa gaaacagatc atgatgatcc aaacagagca attaaaaaca cataataatg
     4441 agcggaaaag acagcaatta ttcggtaacc aacgtattgg aaacgacgtc tatgaatggg
     4501 acgatatcaa catcgccgtc atttctggtg atactatcat agatttgggc aatacgcttt
     4561 tgccaaaaga tgataatatt gtgattgtaa gaaaagggat aggcagaaca cggatcttag
     4621 tacctttggg cgtagccatc cgattagagc atgcgacatt agtaggaaat gtcttgtttg
     4681 aagaagagca attttctttg aaaaatgaac aaatcaaaat ctacagcagt gattatgatg
     4741 aaaaccctcg tagattgaag attattacta ataccttgat tggagatgtc gaggtgatac
     4801 gaatatgatg ggaaaaatat ccagagcgat gctagctgtt tattcgggga ttgcggcttt
     4861 ccttattatt ctattttcac ttttcactta tttttatgcc agcaatcaaa gtcattggtg
     4921 gggagaatta ctgcgtgcac gtttattata tgttccgctt atcttccatt tgttagccat
     4981 ttccttaggt gtaggattaa ttgtctttct attattatca cttattcaaa agacaaaata
     5041 cgggaaaatc gaagaaaagc tgcgtgcact ttcttctggc aattatgaat ccaaattgtt
     5101 gcttcttccg atcccaagtg catcggacga tttatacatc aaagatattg ataaggaaat
     5161 cactaagata aaagaaaaaa tgattgaagt atctagtgaa ttacaaattg taacgagccg
     5221 tccccaatat gtagatggac aaaccaaaga agaaatttta gaattagaaa gacatcgatt
     5281 agcccgtgag ctgcatgatt cagtttcgca acaattgttt gcagcaatga tgatgatgtc
     5341 ggcattgacg gagcaagcag aaaaaagcga gacaccagaa atgtttcgca agcagttgaa
     5401 aatggtagca gagatcatca acgcttccca gtctgagatg cgtgcgctgc ttcttcatct
     5461 acgtccagtc aatttagaag agaaaagttt aaagcaaggt atcgagcagt tattgaagga
     5521 attgcaaaat aaaatccaga tttcgctgaa atgggatgta gaagatgtaa aactatctag
     5581 ttccattgag gatcacttgt tccgaatcgt tcaagaattg ttatcaaata cattaagaca
     5641 tgctaaagcc aatgaattag aggtatattt gcacaaaata gacaataatc ttttgttacg
     5701 tattattgac gacggaacag ggtttaatat gaatgaaacg aaaacgggaa gttatggatt
     5761 gaacaatatc aaagaacgag tagctggtat tggtggaaca gtaaaaatca ttagttttaa
     5821 aggtcaaggt acaagtgtag aaatcaaggt ccctttgatg aaggaggcat agataaggat
     5881 gataaaagtt ttattagtag atgaccatga aatggtgcgc ttaggcgtct ctttttattt
     5941 atctattcaa gaagacattg aagtaattgg agaagctgaa aatggacgac aaggctatga
     6001 gaaggcgatg acacttcgac cggacgtcat tttaatggac ttagtcatgg aagaaatgga
     6061 tggtatcgaa tcaacaaaag caatcttaaa agattggcca gaagctaaaa tcattatcgt
     6121 aaccagtttt attgatgacg aaaaagttta tcctgccatt gaagcaggtg cagcagggta
     6181 tttgttgaag acctctaccg cacatgaaat tgctgatgct ataagggcaa cgcaacgtgg
     6241 cgagcgagta ttagaaccag aagtgacaac aaaaatgatg gaaaaaatga gtcggcgaaa
     6301 tgagcctgta ttacatgaag aactaacgaa tcgggaaaat gaaattttaa tgctgatttc
     6361 tgaaggaaaa agtaatcagg aaatagcgga cgaattgttt attacactga aaacagtaaa
     6421 aacacatgtt tccaatatat tggctaagct agaagtggaa gaccgtacac aagccgctat
     6481 ctatgcattc aaacatggtt tggtgaaata aaaacgatga taaagaggat aatcaaaagc
     6541 tgagaaaata actattattt ttcttggctt ttttacaata atgatgcttg cttttgtttt
     6601 tgtaaaggct ttgttatact gagaaaagaa gatactaaag gaggaaaacg gtgaaacaaa
     6661 attttgcaat tgtaggtctt ggacgatttg gcggaagtat ctgccgaaca ctgatcgaag
     6721 ctggtcaaga agtactggct attgatagta atgaagatcg ggtcaatgaa tatatgaata
     6781 tagccactca tgctgttgtg gcaaatgcac aggatgaaat gacacttcga tcattaggca
     6841 taagaaattt cgatcatgta attgtcgcta ttggagaaga tattcaagcc agtatcttag
     6901 ttactctgat ggtaaaggaa atgggcgtac caaatatttt agcaaaagcc caaaatgagt
     6961 atcatgccag agtacttgaa aagatcggag ctgatcgcgt cgtccatccc gagcgagaca
     7021 tgggaataag gattgcgcat aatttagtgt caaagaatat tttagattac ctagagttgt
     7081 cagataaata ttctttagca gaaatccgag tctccaatcc taaatttttc aacaaaacat
     7141 tagctgaatt aaatttcagg caacgttttg acttaacagt agtcgccata cgaagagtag
     7201 atcaaacggt tatcgcttct cctgcagcag atgaatatgt tcgtgaaaat gataatctac
     7261 tggttattgg agagacaggc gatgtggata ttctagacga taagatgaat caatagaaag
     7321 aaaagagaaa gtaggttgtt gggatgaaat taacggtaac gccaaaagca caaaagtggt
     7381 ttgactcaga aattgatctg ccagaaactt acggaatacg tttttttgga aaagtctacg
     7441 gcaaaacaga ggttcatgat ggtttttcga ttggaatgtc agttgaacag ccagaacatc
     7501 ctgtaaagaa agtggaaatt gatgggacac ttttttttat cgaggaagca gatgaatggt
     7561 tctttaaagg atatgattta cttgttgatt ataatcccaa cctggaggag ccaacctatc
     7621 actttaaaaa acaataaaaa aagagagggt gacacaattt ttgtcacctt ctcttttttc
     7681 ttttgaaaat attgttttca gctattaata tcctgcagac aattttactt gattggtttg
     7741 aagctcatgg gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt
     7801 ttgaaaatca ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca
     7861 aagaggatga tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag
     7921 ttgctggtta tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac
     7981 attgatgaat acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt
     8041 tgtttcctcc gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt
     8101 tagttgattt aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat
     8161 tccatagcag ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc
     8221 agttccaatg attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa
     8281 ccatttcatg tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat
     8341 gattcctaag acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa
     8401 aatatgtttc tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg
     8461 gacccatttc aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa
     8521 actgatctct acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg
     8581 cgctatagta tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat
     8641 tctttctgac atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag
     8701 aactttgaag cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc
     8761 tgtgacaaaa aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt
     8821 ctttatttct taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa
     8881 gattcctgca aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat
     8941 tgttgcaatt atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa
     9001 agtagctttt gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa
     9061 agtgatcagc agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat
     9121 ccaaagtggt atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt
     9181 taaagattca ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt
     9241 gcctgtatta ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt
     9301 ccccatcaaa tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa
     9361 gataatggag tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt
     9421 ttggattgta aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt
     9481 taagacttga aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag
     9541 atagacaatg gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat
     9601 aaaggaactg ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc
     9661 acttgcaatt ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg
     9721 attggtgaaa tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat
     9781 ggaggaagga atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt
     9841 ggttttgaat gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt
     9901 gtctcttgtt tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta
     9961 cggggcacaa aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg
    10021 tttagaaata ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt
    10081 aaccgttgaa gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct
    10141 aacagtagca gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga
    10201 agcagatgaa agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc
    10261 ttcggtaagt gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga
    10321 tccagttatt acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa
    10381 aaaagctgcc gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca
    10441 agaaattgaa gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag
    10501 cgaaaagata attattttcg acggacatat tgattttgaa gatcagctac taagcgagat
    10561 tgaaaagata tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt
    10621 gacgatccaa gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga
    10681 tgaaagttat tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc
    10741 atgaagcagt atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta
    10801 gtctatctct tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca
    10861 cgccaatatt ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta
    10921 ttgtttgctt ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca
    10981 catgcactta ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac
    11041 atgggggcaa tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc
    11101 atcactcaaa ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa
    11161 gaaactgagg tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa
    11221 ttgaagaatt tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg
    11281 aggaggaatt tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg
    11341 gaaccttata ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat
    11401 gtgacgaatt taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa
    11461 gaattattat ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat
    11521 ggtggcggac atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc
    11581 gaacctacag gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt
    11641 aaagaagaat tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta
    11701 atggaaaatg gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa
    11761 ggaaagacac caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa
    11821 aatgtccgac cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac
    11881 aaacgtttcg aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt
    11941 ggaaaataaa agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa
    12001 ggttacttgc aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg
    12061 gttcaacaat gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt
    12121 tcatttctca gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga
    12181 attcacgatt gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata
    12241 atttgttgaa taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt
    12301 ttttagattt tttggctaag taaatgcatt tgttcacttg acatgtccta actaattatc
    12361 aagcaaggaa cattttcttc gttagtaatg gattttaaag aaatttcagt ttttggatgt
    12421 tgaataaaag accatttgtg aaaccactag tatacatact cattaccatg tcaattctat
    12481 tactttaaaa gaaaatcgtt ttactttgaa gaggagcata tctatccatt aaaagatgat
    12541 ggtaatgcaa tcactagttc gagtcatttt aagagccatg cagtgagctg gaaataaaac
    12601 gaatagaaga tgggtttaac aaaaggacca gtcataggaa gaaattgtcg tgaaatcgaa
    12661 tgataatctg aacggtaggc aaagactgtt aaaaagatgg gaaaggtatg tgcgatcatt
    12721 ttactgcacg attatttgga aaataccttg ttgttcgctt tttctggaaa aaatacataa
    12781 aaaaagataa aacgattgcg aactgcgtgt attcggtctt tcgatctttt tcacggtatg
    12841 gtatgatagt taagaatttt gagaaatttt aaaaaataat aatcgttaca caaaaatgtt
    12901 ttttaaagtt ttcttatgat taaaaagtga atttgctgat tgaacagtta tatgagacaa
    12961 tattttagaa aatgtgaaac agaggtgtat tatgaagcga tggcaaaaag ttctattaac
    13021 cttgttaggg gtattcgttt tgctgattgc cggattatca gcatacggaa tcagatttat
    13081 gggagaagcg agccaaacag taaacaaaat ttctaaaaac tctaatcgaa tatcctccaa
    13141 acgttctgag agagtaagca ttgatgacaa agaacctttt tcggttttgc tattaggttt
    13201 agataccggt ggattaggaa gaacggaaca aggacgttct gatacgatga tggtagtaac
    13261 agttaatccg aaacaaaaaa aatcaacgat catcagtttg gaccgagata tttatacgaa
    13321 tatcgtaggt tatggaacgg tagataaatt aaatcatgcc tacgcatttg gtggtgtcga
    13381 aatggccatg gattcaattg agcaactgct cgatattcct attgatcatt atgtaacgat
    13441 caatttggat ggcatggaag atttgatcaa tgcggtcggt ggcatcaaag tgaataataa
    13501 aattgacttt actttggatg gtgtccatgt accagtaggt gaacaaacat tgaatggaga
    13561 aaaaggttta gcctattctc gaatgcgaca cgaagatcca gaaggcgata tcggtcgaca
    13621 acgtcgtcag cgggaagtag tgacgaaaat cgtcaataaa gttttaagtt tagatggcgt
    13681 aagcaattat agaaaaattt taaaagcagt tgagaacaat gtgacgacgg acttagattg
    13741 ggatgacatg cttgatgtag cgacgaacta caccccggcg tttgaaacaa tcaaacaaga
    13801 ccagttgcaa ggcgaaggac aaatgatcaa tgatatctac tatcaaattt taggaaaaaa
    13861 tgatcttctt tcgatccaaa atgaactgaa gaaacaatta aatataaaaa caagcgatac
    13921 attacctaat ttgaaaaatg agaatgcttc aatgatgttc tttgatgatt cggaagatgg
    13981 cgattcgaca aacggtgatg gatcgaatag tgaagggtac accgattatt cttcctattc
    14041 agaaaacaca tatgatcagt accaagatcc gaatacctat tatcagcaaa cagaagatac
    14101 aagtcagtac tatacaccaa gtcaagatac tcaaagttat gatccttacg ctggacagga
    14161 acaaaatggt tatggttatg accagaataa tgagtatgga acaacaagtg ggtactagaa
    14221 aaaaatagtt tggttaataa agagtgccac ctaaattaga caaaaaatct aacttaggtg
    14281 gcactttttc tatgtgttaa attacaggac gtctcttttt agcgcttagt ttaaaaaata
    14341 aattgaataa ttcaatagag ttgcgaatag tacccataaa taataaggaa tcatgagcca
    14401 cattgccagg cgatgatgca gccacaactg atacataagc actgacaaga aaataactaa
    14461 aagtgtaata tcgattgctg caatgaaaat atttcttaaa ttaaaaaata gagcagacca
    14521 tataaaatta aaaatgaact gaatcacaaa aagaccgaca agattgtttc gctgtttttt
    14581 gttgttgtga ttaagtaaca a
//
LOCUS       DASJSZ010000066        14530 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.067, whole genome shotgun sequence.
ACCESSION   DASJSZ010000066 DASJSZ010000000
VERSION     DASJSZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.067"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..360
                     /locus_tag="U4B03_002006"
     CDS             <1..360
                     /locus_tag="U4B03_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEN2068848.1"
                     /translation="AKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKIKKMLLGK
                     TIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQFIAADYDA
                     DLADQFINGNNFMPTTN"
     gene            complement(491..967)
                     /locus_tag="U4B03_002007"
     CDS             complement(491..967)
                     /locus_tag="U4B03_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEN2068849.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(927..1274)
                     /locus_tag="U4B03_002008"
     CDS             complement(927..1274)
                     /locus_tag="U4B03_002008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068850.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(1261..1446)
                     /locus_tag="U4B03_002009"
     CDS             complement(1261..1446)
                     /locus_tag="U4B03_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068851.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1447..1719)
                     /locus_tag="U4B03_002010"
     CDS             complement(1447..1719)
                     /locus_tag="U4B03_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HEN2068852.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1716..1919)
                     /locus_tag="U4B03_002011"
     CDS             complement(1716..1919)
                     /locus_tag="U4B03_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068853.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            2044..2256
                     /locus_tag="U4B03_002012"
     CDS             2044..2256
                     /locus_tag="U4B03_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068854.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(2289..2483)
                     /locus_tag="U4B03_002013"
     CDS             complement(2289..2483)
                     /locus_tag="U4B03_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068855.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(2496..2678)
                     /locus_tag="U4B03_002014"
     CDS             complement(2496..2678)
                     /locus_tag="U4B03_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068856.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(2675..2815)
                     /locus_tag="U4B03_002015"
     CDS             complement(2675..2815)
                     /locus_tag="U4B03_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068857.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(3305..3670)
                     /locus_tag="U4B03_002016"
     CDS             complement(3305..3670)
                     /locus_tag="U4B03_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068858.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(3710..4012)
                     /locus_tag="U4B03_002017"
     CDS             complement(3710..4012)
                     /locus_tag="U4B03_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN2068859.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(4012..4311)
                     /locus_tag="U4B03_002018"
     CDS             complement(4012..4311)
                     /locus_tag="U4B03_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068860.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(4304..4468)
                     /locus_tag="U4B03_002019"
     CDS             complement(4304..4468)
                     /locus_tag="U4B03_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068861.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(4474..4743)
                     /locus_tag="U4B03_002020"
     CDS             complement(4474..4743)
                     /locus_tag="U4B03_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068862.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(4755..5504)
                     /locus_tag="U4B03_002021"
     CDS             complement(4755..5504)
                     /locus_tag="U4B03_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2068863.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(5501..6193)
                     /locus_tag="U4B03_002022"
     CDS             complement(5501..6193)
                     /locus_tag="U4B03_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068864.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(6199..6870)
                     /locus_tag="U4B03_002023"
     CDS             complement(6199..6870)
                     /locus_tag="U4B03_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN2068865.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(6863..7204)
                     /locus_tag="U4B03_002024"
     CDS             complement(6863..7204)
                     /locus_tag="U4B03_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068866.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(7382..8080)
                     /locus_tag="U4B03_002025"
     CDS             complement(7382..8080)
                     /locus_tag="U4B03_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEN2068867.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            8167..8637
                     /locus_tag="U4B03_002026"
     CDS             8167..8637
                     /locus_tag="U4B03_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068868.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(8680..8859)
                     /locus_tag="U4B03_002027"
     CDS             complement(8680..8859)
                     /locus_tag="U4B03_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068869.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(8846..9184)
                     /locus_tag="U4B03_002028"
     CDS             complement(8846..9184)
                     /locus_tag="U4B03_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEN2068870.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(9200..9403)
                     /locus_tag="U4B03_002029"
     CDS             complement(9200..9403)
                     /locus_tag="U4B03_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2068871.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(9418..10194)
                     /locus_tag="U4B03_002030"
     CDS             complement(9418..10194)
                     /locus_tag="U4B03_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEN2068872.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            10493..10813
                     /locus_tag="U4B03_002031"
     CDS             10493..10813
                     /locus_tag="U4B03_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2068873.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            10831..11262
                     /locus_tag="U4B03_002032"
     CDS             10831..11262
                     /locus_tag="U4B03_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2068874.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            11392..11853
                     /locus_tag="U4B03_002033"
     CDS             11392..11853
                     /locus_tag="U4B03_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEN2068875.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            11887..12522
                     /locus_tag="U4B03_002034"
     CDS             11887..12522
                     /locus_tag="U4B03_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEN2068876.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            12635..13270
                     /locus_tag="U4B03_002035"
     CDS             12635..13270
                     /locus_tag="U4B03_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068877.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            13569..>14530
                     /locus_tag="U4B03_002036"
     CDS             13569..>14530
                     /locus_tag="U4B03_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2068878.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
BASE COUNT         4513 a         2689 c         2263 g         5065 t
ORIGIN      
        1 gctaaatctg agcaattgga acaaattgct ggagttggtt atagaaaagc tctagaattt
       61 ctaattaaag attatgcaat atctaaaaat tcagaggatg aagataaaat aaaaaaaatg
      121 ttattaggta aaacaattga aaattatttg aatgattttc ctaaactcca aagattagcc
      181 aaagctgcta cttggattgg taatgatgaa acgcactatg ttcgtagaca tactaataaa
      241 gatgttcatg atatggatgc atttattaaa tcagcagcgc aatttatcgc ggctgattat
      301 gatgctgatt tagctgatca atttataaat ggaaataact ttatgccaac tactaattaa
      361 aagaataaaa gagccacaga taccaattaa taaaacggtt gggatatctt ggctctttca
      421 tatttttttg acactaatag aatatcatgc tgctaacaat gtttacgaac atttagtgaa
      481 cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg taaaaatcaa
      541 tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct aaaaagttca
      601 atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac ccaacagatt
      661 cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt tgattatcga
      721 taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc aacaattcca
      781 attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa ttcttttcga
      841 tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta cttttattaa
      901 gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt aaccaatgaa
      961 atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc gaaaggggta
     1021 gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca aacgtcgttt
     1081 tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga aaacacattt
     1141 tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc tttactcatc
     1201 tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg aacaggtaaa
     1261 tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg cgttagtgcc
     1321 gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt ttgatttata
     1381 gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta agatgtatac
     1441 tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct gcatgtaatc
     1501 agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg ttggtctcgt
     1561 tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta actgatcacc
     1621 aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc tcgtcactgc
     1681 ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc cttcaaaatt
     1741 cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt atcgttcatg
     1801 tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt aacgatcttt
     1861 attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac tttcaccacc
     1921 gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa tcagcttttt
     1981 tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa aaggtgattt
     2041 atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga atcaggatta
     2101 gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga agacgacatt
     2161 cctactctta aacaattaaa atattattct gattattgga cgcaaactcg tgagaatgaa
     2221 ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac acggctggtt
     2281 ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat aatctgatca
     2341 tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt gtaagttttc
     2401 aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt ccctgtttgg
     2461 atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc tagatggagc
     2521 aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt gctcggctta
     2581 actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca aataaatttg
     2641 gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga aaaatgatgt
     2701 cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta gctaattcga
     2761 tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca ttcatactat
     2821 cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt gtgactgctt
     2881 gactttctcc acgctcataa tattctacag ttgatactga tacatcagct tcggagttta
     2941 attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc accttttttc
     3001 ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc tatattatgt
     3061 gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg aaataactct
     3121 caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat tttccgagag
     3181 ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag tcgcgtaaag
     3241 aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc attgatattg
     3301 gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga tctgtgttga
     3361 tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct tcaagacgac
     3421 aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc atatcccaac
     3481 agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt tcacgaatta
     3541 caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat tgattaagaa
     3601 atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc acttgttttg
     3661 catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat tatttcaggt
     3721 cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg atttcgtcat
     3781 aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag atgatcaagg
     3841 ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta gctagagaag
     3901 cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa tccgcttgat
     3961 ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc attatttctc
     4021 ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag ctctatccca
     4081 aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac ccgttactag
     4141 aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg gcttttcagt
     4201 ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt cctgccaatc
     4261 ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca tcccgacatt
     4321 ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca tgaaatgtgt
     4381 tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc attaattcga
     4441 tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt cattaagatg
     4501 tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta aatttcttaa
     4561 ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac tcgaacgatc
     4621 attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc tcaggacgtc
     4681 taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat aatcgatctt
     4741 catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc tagcgctttt
     4801 ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg tcttcctgtg
     4861 cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc ccaacttcga
     4921 atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc tgacagtcgt
     4981 tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat ctgctcatct
     5041 gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc cttcgatttt
     5101 ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt attaatagat
     5161 gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc aatagggtat
     5221 tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta aatgatttcc
     5281 cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt ctctttattc
     5341 acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct ttcattacac
     5401 aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt gtcgtacctt
     5461 acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc cctccaatat
     5521 ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat ttatttttga
     5581 aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt gctgcaaatg
     5641 tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg tcaaagtgat
     5701 cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg catttgtaga
     5761 agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga atatgatgtt
     5821 caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca gttgtattcg
     5881 attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag tagaagaatt
     5941 ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc agcgcataga
     6001 tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt tcaatgactc
     6061 gatcaccatc gtagccgtca tacatcgtct ttagtcgatc aatgttcact tcctcattga
     6121 tggttgcgct tatgtctttt cctttgaact ttttcagaac cgctgaatat gaatcgatta
     6181 atggtttaaa cactcatatc acttcttatc taattctttt ctcttagctg ctattgctcg
     6241 ctccatcaag gcacattgct catagcttaa ctgttcaata gtttcaacgt tatcagctaa
     6301 gagccctaat ttatctgtct gctcattaac atattcgatt aaggttttgg tcatatcttt
     6361 acccatctgc tcattgaaag cttctagaat cgtctctagc atgtttaatt tctttgtatc
     6421 gattctaggt ggtgttggaa tatcttcccc ttgaaataca tataatccca gtccgtgtag
     6481 agccaatgct ttcacaaagc atcgcttcaa tgagttattg atttgcattg catttggttt
     6541 aacaactggt tggtttcgat aatctaaaac aggaaataat tcggtttccg tgtgtccttt
     6601 aaccgttact gagacagata cataagtccc agtttcatcc ataagaaaag gtttatattc
     6661 ctcaacaaga aagtcttgat gagttccaga aacaaccctg tagtgtttgt actcattaat
     6721 agttaccgtt gcctgtggat cattcttttt cataatctcc cacgcgtgag cccaagataa
     6781 ataatcaaaa tttccttttt tcttgagaat tttatttaac ttacgactaa aaagtttttc
     6841 aaagttcgtt gtccctttga tttcactcat caaattctgc ctccatttca gcaatgtatt
     6901 tcttacctgg tccgtaataa gagatatcaa tcaagttatc tctgtcgtac tcttctagcg
     6961 catcaatcaa gccatcttcg atgacgtaaa tatattcagg tttattcgaa tgcttcgata
     7021 gatggataag ataaacatga tcccaaatag ttacatggtt gcctaaatca tcttgatccc
     7081 aagctagttc ttcattcgtc aaaagatttc gtctgatttt tcgaccactt gtttcctcaa
     7141 ttttcggctt gccccaatca ggatcaatca aatattgatc tagagtggaa agttcttttt
     7201 ccatatgcta aaatctcctt atgatgtgtt ttctttgtga ctctttgctt gccggcggag
     7261 tcactttttt atttgttgcc atgctttttg cttgtcaata tgttgttggc ttaggatgat
     7321 tggtttatag tatttccacc agcaattagc aattgccgtc cctattctta gcgcttcagc
     7381 tctattcatt gtcatcacca aaaagtctct gttgtctgtt cagttgatca atttccatac
     7441 ggatcgcagt ttctggcaac cacatttcaa taaatgaaac agcatcatcg aatctcttac
     7501 gaggtaactc gccatatctt gggattgaaa aggtacgttt aaattcagac caaaattttg
     7561 agaatacttt tttgctgatt tcttcataag ctcggctttc ttttccccct agaactccca
     7621 taactttcat atttcctttt tgcttaattt caaactcttg ttgtccgcta attcgcatag
     7681 tatctttaag catggaaaca tcttttttaa catctttcat ttcttctaat tgatagatca
     7741 tcatatcttc aattgtttga ggaacagtat tcttgcgaat aacatcttcc atttcgttga
     7801 atgcttcaat gtatttttgt ttgaagtaga tagctttctt tcctgtaaac cccatagcca
     7861 gcaagaaaaa tccatctcta ctaatgaaga aaactcgtcg atttctgcca tatgaatctg
     7921 gttcattacc ttctacaaac atctgtccaa aattggacac atcttttttt agtgcatcaa
     7981 tatctcttaa aacatgttga tgttttttct cgaagctttc tgccacttgt aagctcgtag
     8041 tcacagcttc tttatttttc aaaattacta attcttgcat tatttcttct ctcctttttg
     8101 gtataattta gttaaaaact ggtggtgttt attttgaatt tttttcactt aacaattttt
     8161 caatggatgt caataataag ttttatattg agtacgtttt taacagggat aaaaataaaa
     8221 ggaatgcgcc ctcaactaga agttgaactt aatgcttctt acttcgctgc agatatgatt
     8281 tatacaaaag taatcatttc taactattca acagaaccag ctatgttagt taatttagaa
     8341 ctttcatccg ctaacttaaa ccgcagatgg tcagctactc catttaagaa attaattgct
     8401 aaaggaggat cagtcgatga taatagaata tactctgaag ctgtaccgct aaatattcct
     8461 cctaaaagtg ctatatcttg ttaccttgca tttgaagtag ggaaaattaa ttttaataaa
     8521 ctccttaatc accaaacaaa aatgatattt actctaaata gaacccaaat ccacaaaatt
     8581 gttgatatca aaaatacaaa cttcccaata gagaaattag tgaaagaatt aaactgagta
     8641 atagtaatat acattctagt ttctcttttc tattcatttt tagtcagccc cctcggttgg
     8701 ctttttcgct ctgtactcag cttcagcttc atcaagcccc ataaaaatcc aaaccatgta
     8761 aacaatcgtt cctatcaacg cttgcctgct tccccaaagt cctaaagcgt agatgattag
     8821 cggtgcgctg aacactagcg ctctattaaa ttttcccatc cgcttgcctc cttaagattt
     8881 ccgaaaaatt tgtttctaaa aattcaagtg ttttacttct taaaaataga tatgtgtctc
     8941 ttccttcaac tggataatag acaaatccat ttttgttttt ctcgatatcg ataatatttc
     9001 tatatcttgg gttctttaaa actctagaag taaaccaatc atattttctg ttaatccgtt
     9061 ctagcacttc tggcaacgtc atccatctac cagtatcatc agcttttttt aactcctcat
     9121 aatccacttg ggagataatt acatagcctt ctggaattgg gatttttgct tctagatatt
     9181 gcattagctg ttcctccctt tacaattcgt acatagtgat aatcgaatct ataattctgt
     9241 ttgcttctgc agaagtcttt ttaccgttta aaattaaaga taagtagctt ttgctaattc
     9301 caaatctttc agcaagcatg gtgtaagtta agaactttga actttcgaca tattctttga
     9361 ttttttctct atctcgttga gtgatttctg caatgtcagt catactaaaa ctcctttcta
     9421 accaatttcc tctaaatcca tttgagggta atatccttct ttttttagta attcgtagat
     9481 aaatagacgc cctttctgtg tccatttggt attcattaca attttagtgc caccatcggc
     9541 tttcgggatc tcagttgtat gagattttgt gtatccttgg ttcatgtgtt ttttgcacaa
     9601 taaccattgg ttaccgactt ttttctgaat acctagttta tgaagtaatt tattcatctg
     9661 ttgtggagac atcccataat ctgctgcaat ctgactaatt gttactgaat ctgtagaaga
     9721 taatatgcta tctaaatagg agattttagg ttcgtactct gcaatctttt gttctgcgat
     9781 taatcttcca gttctttctt cttttagttg agttgctaat tgaatgattg tatctggatt
     9841 aagcaaagct tcttctactt tttctggagt tagataacct ccatgttttc taattgctgg
     9901 caacacttca cttgttaccc aacgtttaaa ttttttggca gagggaagtt ttgattttaa
     9961 gattaaactg tacaaaccag attcgttgat gattgtcatc tctcttgatt gacctgaggt
    10021 cgtgattcgc gaccccatct tatcttccaa atctacatgc cgcgacaaag catctttagt
    10081 gtttgaataa cccaaaacac tcgcaacgtc tttgcctaca aaatatggtt catcatttac
    10141 taaaattgtc cgaacttcgt tttgttcgaa attaaaaatt tgcggtgtgt tcatatttct
    10201 cattcctttc ttttatattc gtaaacaaat ttaacaactt ttttctaaat tcaattgaca
    10261 gtatctgagt aatactctat actaagagca taattaaata agacataaaa acattgattt
    10321 taaagctttc ttggcggtct gcatttacat atcaatagtg ttttttgttg tctttttagt
    10381 tgttaaatct gtttacaaga tatagtatag ctcattactc agaattagtc aaattatttc
    10441 tgcgtatttt tctaaacttt ttttgtaaac atttagaaag gttgttttat cgatgaatac
    10501 ctatgaaata ataaaagact tggcgaagag gaaaaagata tctatacggc agttagaaat
    10561 taattttgga tattctaacg gttatctagc aaagtggaaa aataatactc ctaattcaga
    10621 tgaattaagt cggattgctg actatttcga tgtctctgta gactaccttc taggtagaga
    10681 agaaagagaa acccctaaac atgtggattt atcagaagac gatactgttt tttcttttga
    10741 tggaaaagaa atatctaagg agacaatgcg taaagcgatt gcaattgcta aagctttaga
    10801 ggaaaatgaa tagttggagt gatggtttgt atgtatttga aattgaaaga aatgttgagc
    10861 gaatacaatt tgaaagtagt ttatatggag atgaaagagc ctggtttcta ctatccaaaa
    10921 cccagaatca tttttttgaa tgaaaatcta tacggtgaaa ctgcagaagc ctttcattta
    10981 tctcatgaac ttgcacattt cagcgcctct cattttgaat tttcagtttt gtacgatact
    11041 tctacaactt ttcactcaaa atttgaaaca gaagcagata aaatagctat tctaatttta
    11101 ttaaatatct atattgagaa cgaactgact gatgaatctc agtttaatct cgaaaaattt
    11161 atggagtatt attctattca gaataagctc agatacactt gttatgctgt ctgccaatgc
    11221 tattttaaga aaaaatattc ttacgcaagg caatatgtat gaatacatca agaattatta
    11281 aaaaaaccga tttcagcaaa acattaaaaa agcccgtgct gcaacacggg ctctttcctc
    11341 atatatgagc ttctacaaaa aaaatcatat cacagaaatg aggaagaaaa aatggaaaaa
    11401 gagattttaa taaaatctcc aggaaaaacc ttgattaaag taactgatga ttctatttca
    11461 attattagaa aaggatttat aaatttagtt aatcaaggta ttaaaggaga aaaaactatc
    11521 ccctttaaaa atatatcagc cgtacaatta aaaaaaccag gtatgagtaa tggttatata
    11581 caatttacct tgctaggcgg aaacgaaagt cgcggaggta tattagcggc cacgaaagat
    11641 gaaaacaccg ttatgtttac aaaaaaatat tggaatgaaa tggaaaattt aaaaaaatat
    11701 atagagaagc aacaatctat attagataat aattccaaag aaacacaaat ttcaagtgca
    11761 gatgaaatta aaaagtataa agagttgtta gatgaaggga ttattaatca agaagaattc
    11821 gattttaaga aaaaagaatt gcttggttta taatattcaa tatcaaaaga gaaagaggat
    11881 ctattaatgg acggtatatt tggttttact ggtattgttt tatttattat aggtttaatc
    11941 atgttaattg taagatttat caaaaaaaca aataagaaaa cacctatgat tctccttatt
    12001 atcgggatta tttttactac cgtaggtttc tcgctttcct catcaaacga ggcaaatgac
    12061 aataacaaac aggaaagctc atcatctgat actcaaacta ctacatctaa cagcagcaat
    12121 aaaagcctaa ctcaatttga agaattttta gagacaaata atcatgactg gggtgctttc
    12181 ttagatagtt attattctat tactccagct actgagcaaa atacagcttt tactaaatat
    12241 attagtggta aaacatatac ttttgaagga actgttatcg aatcaatgac tactagaata
    12301 gccattatag cagacaaaga gtacgatgat aaaagttgga atgatatttc tgcaacacct
    12361 aaagtttcat atgtaatttt tgcgaaggac ttaaacaatg ctgatacctt tacaaagggc
    12421 gataaagtaa cttttacagg agaaattagt tcaagaggat cgaacataga aaaatcctat
    12481 gctcaatggg atatgattaa tagtaaagta agtaaaaaat aaatgaacca cgccccacta
    12541 agagttagat tgtgcacaat aataaaacct atacaaaaga gtgccatcag gagtcacaat
    12601 gcttgtaaag gaaatgacaa aataggaggt aactatggat aatagcaatg aaaaactaac
    12661 caaaaaagaa tatcttgaat taatagttga aggcggtatt ggagcaattc cttatattgg
    12721 tggcgttgtg cagtcacttt attttgggtc taaaaacgag aagcgtttca aacgcatcga
    12781 gaaattctac aaaaatttaa acgaatcatt agaaaacatt cgcgatcaaa ttccagaaga
    12841 tgttttcaac ttagagaaca aggaccaact cattggaata ttcgaaggga taaatgacga
    12901 ggtagaaaaa tcaaaggcac aaaataaaat agatatgtat aaaaatcttt ataagaattg
    12961 tttacttcga attaacaacg cttcatggga taacgaggag tattatcttc ttgtactgaa
    13021 tcaattaaca tcgattgaga ttcaattgtt agcatgctta atgagtcgtg gaaacgaaaa
    13081 tttcactgga aatatcagca tgtctggcta ttcacaagag ctaatagatg gtagcttaaa
    13141 tagattatct gattttggtc tgttagaaaa aacgattagc tccatagtat taggtgatgt
    13201 cggcaaacaa aatatgggct acaaaatatc tcatttagga attcaattta ttaactcaac
    13261 tcttacttaa gttttctttc taatatgaca tctagaccta tcagcaatgc aacaataaaa
    13321 aaccagacat accaataatc aacaaataat ctaaactctg gaagttcagt tattctttta
    13381 aaaatcactt taatcataat aaacacctct gattggatga tctaatacac atccaattat
    13441 accattaaaa cgacaaatag cacatccttg cgccggcaag caaaaaggat gtgcaaaaaa
    13501 taataaaacc caaacaatgg gcttctttat agttcctatt gtatcagaga aagagagctg
    13561 attcaattat ggcaagatta gtcaaacgtg gaaatagttg gcaatacgaa atttcataca
    13621 aaaaagatga cggaaaatac acgaagataa gaaaatcagg atttaagaca aaaggagaag
    13681 caaaagatgc cgccaacgaa ttagaatata acttgaacaa aggccttaaa ggggatcgca
    13741 aaaatctatt attatcagat tactttgagg attggatgca actttataaa gaaggaacag
    13801 tatctcctat cacttataga aaatacgaag atacgttaat gaacataaag aaatatatgc
    13861 cagcggtatt gatttctgat ttagatagag tgggatatca acgcttttta aataagtatg
    13921 ctaaagacca tgtaaaatct accgttatta agtttaataa ccatattaga gcgtcgttga
    13981 aagatgccgt agaagaagga ttaattccgt ttgatccaac tagaaaagca gtaatcaaag
    14041 gaaaagattc attgaagcca aaagaagata aatatttaga ttatgatcaa tttaaatctt
    14101 taatgaaact cgtagaagaa aacctttctg cgcagtactc ttctcctatg ctcgtgttag
    14161 ttgctggtgc cactggaatg cgatttgctg aacttctagg attaacatgg gaagatatcg
    14221 atttcgaaga tcaaatcatc actattaata aaacatggaa ttataaatta aatgaatggg
    14281 gaaaaacaaa aaacgaaact tcaaatagga aaatttccat tgataaacat acgattgatc
    14341 tcttaaaaaa gtttaaaatc aatcaaaaag aattattcga gaattttgaa atcaaaaacc
    14401 ctcataattt tgtttttttc aacttaaaaa atggattagt ttcatcaaac gccgtcagca
    14461 aatatttgcg caaaaaatta aaagaattag ggattgaaaa gcaatttact ttgcatggac
    14521 taaggcatac
//
LOCUS       DASJSZ010000067        14136 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.068, whole genome shotgun sequence.
ACCESSION   DASJSZ010000067 DASJSZ010000000
VERSION     DASJSZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.068"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1835)
                     /locus_tag="U4B03_002037"
     CDS             complement(<1..1835)
                     /locus_tag="U4B03_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2068879.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="U4B03_002038"
     CDS             complement(1832..2536)
                     /locus_tag="U4B03_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2068880.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="U4B03_002039"
     CDS             complement(2548..4857)
                     /locus_tag="U4B03_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN2068881.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="U4B03_002040"
     CDS             complement(5052..5516)
                     /locus_tag="U4B03_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068882.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="U4B03_002041"
     CDS             complement(5516..6142)
                     /locus_tag="U4B03_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2068883.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="U4B03_002042"
     CDS             complement(6149..6514)
                     /locus_tag="U4B03_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN2068884.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="U4B03_002043"
     CDS             complement(6504..6842)
                     /locus_tag="U4B03_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068885.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="U4B03_002044"
     CDS             complement(6832..7158)
                     /locus_tag="U4B03_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2068886.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="U4B03_002045"
     CDS             complement(7139..7417)
                     /locus_tag="U4B03_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN2068887.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="U4B03_002046"
     CDS             complement(7419..8783)
                     /locus_tag="U4B03_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2068888.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="U4B03_002047"
     CDS             complement(8796..9365)
                     /locus_tag="U4B03_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEN2068889.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="U4B03_002048"
     CDS             complement(9337..10524)
                     /locus_tag="U4B03_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2068890.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="U4B03_002049"
     CDS             complement(10588..12315)
                     /locus_tag="U4B03_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2068891.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="U4B03_002050"
     CDS             complement(12312..12764)
                     /locus_tag="U4B03_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEN2068892.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="U4B03_002051"
     CDS             complement(12876..13256)
                     /locus_tag="U4B03_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2068893.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="U4B03_002052"
     CDS             complement(13253..13639)
                     /locus_tag="U4B03_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068894.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="U4B03_002053"
     CDS             complement(13641..13919)
                     /locus_tag="U4B03_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068895.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
BASE COUNT         3904 a         3051 c         2224 g         4957 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttgg
//
LOCUS       DASJSZ010000068        14119 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.069, whole genome shotgun sequence.
ACCESSION   DASJSZ010000068 DASJSZ010000000
VERSION     DASJSZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.069"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..767
                     /locus_tag="U4B03_002054"
     CDS             75..767
                     /locus_tag="U4B03_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN2068896.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
     gene            complement(1049..1121)
                     /locus_tag="U4B03_002055"
     tRNA            complement(1049..1121)
                     /locus_tag="U4B03_002055"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1086..1088),aa:Lys,seq:ctt)
     gene            1502..2071
                     /locus_tag="U4B03_002056"
     CDS             1502..2071
                     /locus_tag="U4B03_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEN2068897.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2058..2918
                     /locus_tag="U4B03_002057"
     CDS             2058..2918
                     /locus_tag="U4B03_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2068898.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3108..3812
                     /locus_tag="U4B03_002058"
     CDS             3108..3812
                     /locus_tag="U4B03_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2068899.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3817..5652
                     /gene="walK"
                     /locus_tag="U4B03_002059"
     CDS             3817..5652
                     /gene="walK"
                     /locus_tag="U4B03_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEN2068900.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5649..6965
                     /locus_tag="U4B03_002060"
     CDS             5649..6965
                     /locus_tag="U4B03_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068901.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6966..7835
                     /locus_tag="U4B03_002061"
     CDS             6966..7835
                     /locus_tag="U4B03_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEN2068902.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7890..8699
                     /locus_tag="U4B03_002062"
     CDS             7890..8699
                     /locus_tag="U4B03_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2068903.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8802..9431)
                     /locus_tag="U4B03_002063"
     CDS             complement(8802..9431)
                     /locus_tag="U4B03_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEN2068904.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9601..10893
                     /locus_tag="U4B03_002064"
     CDS             9601..10893
                     /locus_tag="U4B03_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2068905.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10969..11631)
                     /locus_tag="U4B03_002065"
     CDS             complement(10969..11631)
                     /locus_tag="U4B03_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2068906.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11856..12140
                     /gene="groES"
                     /locus_tag="U4B03_002066"
     CDS             11856..12140
                     /gene="groES"
                     /locus_tag="U4B03_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEN2068907.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12191..13816
                     /gene="groL"
                     /locus_tag="U4B03_002067"
     CDS             12191..13816
                     /gene="groL"
                     /locus_tag="U4B03_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEN2068908.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4806 a         2375 c         2987 g         3951 t
ORIGIN      
        1 atatatattt aaattgactt attgtgaaat cattaactat aattagttca gtttatgtta
       61 aggagaatga tgatatgaat aagaaaattg tagtaagtgg attaattata ggtggattag
      121 tgttagcagg gatcgcaggg aaaaactttg ccgatgacat tttgttatgg ggtggagaag
      181 ataatattga acaaatcaat cataatttgg agtcattaga taaagcacta gaaaataaag
      241 agcaaaatat tagtagttta aattcacgat tatcttcaaa taatcaagaa cttgagcaag
      301 caaaggctaa cgttgaagaa tataaacaaa aagtgttgac tttggaaaat gataaaaatc
      361 aattaactat agagaaaaca aatttagaga atcaattatc aggaaaaaat gctgaattgc
      421 aaggtaaaca aaatgaaatc aatgctaaaa ttgatgaaat aaatcaaaaa atagctgaaa
      481 taaatcaatt aaacactgat tggaataatc gtttacaaac agaacaaaat aaatttaatg
      541 atgctcaaaa tactgttaat agtttaaatc aacaaattaa tcaattgtca aatgaaaaaa
      601 atactattaa aaaccaattg caaagtactg aacaaaaact agaaaaagcg caatcagaaa
      661 acagtaattt gaataattat attgaaaaac tggaaaaagc caaaagcgat gtagaagaca
      721 cagcaaacag atcacaagaa atcgtgactg aacatacagg aaaatagatg cattttttag
      781 tttaaatttc tttaacaaaa tagtattttg ttgctaaata atttttattt gaatattcta
      841 caatgatatc tcccctcaaa atggagggga agtacatact gtgataatct acaggtttat
      901 tgactgatta ggatgttatt aaaaaataaa atactaaaat gcatacccga atttcatacc
      961 cgaaaaataa aaaatgccac cacgtacact cttttcgaaa ctacctaagt agccaagaat
     1021 gtactggtga cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc
     1081 tgattaagag tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata
     1141 atagtagcac taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt
     1201 tacttcctta taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat
     1261 accaacaaac aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc
     1321 tttcccgccg tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag
     1381 ccttcttgtt ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta
     1441 gattgaatgt ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat
     1501 gatggaaaaa atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct
     1561 gcctcactgg gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt
     1621 tgatcgttat ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat
     1681 ggtaaacaat tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa
     1741 agaacacgta gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga
     1801 aatcaaagaa gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct
     1861 attttgcgat gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc
     1921 tcatccgcaa aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac
     1981 tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt
     2041 aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg
     2101 atgtgccaga agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt
     2161 ataaagatca ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt
     2221 tgccagagga aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg
     2281 agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga
     2341 gcgggacata taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg
     2401 tactagatac gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat
     2461 ggctggaaga aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa
     2521 gaacaaaagt tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg
     2581 gtttagttgc ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg
     2641 aagaagggat ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa
     2701 cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc
     2761 atggggatgc gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac
     2821 aagcaaacca aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg
     2881 gattattggg tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag
     2941 gtgaaaatca gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg
     3001 cactttcatt ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata
     3061 cggtataatg agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt
     3121 tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag
     3181 gctatgatgt ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag
     3241 aacctgattt gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc
     3301 gtgaggtacg caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa
     3361 ttgataaagt cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca
     3421 accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg
     3481 aaccagaaga ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag
     3541 cttacatggt gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac
     3601 tcttttactt ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg
     3661 tttggggtta tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc
     3721 gtgaaaagat cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg
     3781 gttactattt gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt
     3841 cgatcagtca attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa
     3901 attattggtg cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa
     3961 gatatgaatc aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa
     4021 gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg
     4081 actagcgata tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc
     4141 gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc
     4201 acgactaaac gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa
     4261 ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc
     4321 gaacaaaaat attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt
     4381 gcggcattta tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc
     4441 ggtgaaatga gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa
     4501 gtgcatggac aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt
     4561 attgaagaag ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc
     4621 catatgacag acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat
     4681 atggctgttt ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca
     4741 cttttagata tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg
     4801 gtacttgacc gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt
     4861 tctatgatcc gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg
     4921 acagaacaag aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa
     4981 cttcgtacac cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg
     5041 aaagacgagg aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg
     5101 attcgaatga tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt
     5161 caattagaat atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg
     5221 atggtgggaa atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt
     5281 tgggtcgaag tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg
     5341 atcaagtatt caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac
     5401 gtggttttaa gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg
     5461 ttcgagcggt tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt
     5521 ggattagcta tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc
     5581 aaggaaggaa aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa
     5641 tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt
     5701 taagcatata cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg
     5761 tagatgagac aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct
     5821 ttcttccgct tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa
     5881 atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag
     5941 tagtttcagg agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt
     6001 tggcctataa tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag
     6061 atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc
     6121 aaggaaataa aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata
     6181 tttcgttgaa ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag
     6241 aaatgacaac agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac
     6301 tgaaaaagta tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt
     6361 tccagaaacc agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg
     6421 ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc
     6481 cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag
     6541 gcagttcttt agggaacctc agatattttg accgcagtga caatacgatc gattatcgga
     6601 tattcgtgga aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag
     6661 ttggcggtga aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc
     6721 aagtgccgat accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc
     6781 agttaaaagc taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat
     6841 ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat
     6901 ataaagataa ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga
     6961 attaattgga tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg
     7021 tgtttttatt cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta
     7081 atcagactga atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga
     7141 tttcgtctga gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt
     7201 cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag
     7261 acggtgatgt tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc
     7321 cagataaaat tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg
     7381 gtaatgatta tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc
     7441 aagcaagcca agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt
     7501 ctttagaaaa aaacgataat gattatagca tcacgaaata catgcagacg catttatctg
     7561 atatcgagca gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat
     7621 tgtacatcaa taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt
     7681 cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg
     7741 aaacaagcga taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga
     7801 caaacaatac tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt
     7861 tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca
     7921 gtatcctagc aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc
     7981 ttttggtcga tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc
     8041 ggacaccgga ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg
     8101 gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg
     8161 aagcgatgga accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg
     8221 ggaaagtcag aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag
     8281 cggctccgca attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca
     8341 ccggatattg cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg
     8401 aaagtaatca tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc
     8461 gtattttagg agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg
     8521 tgatcggtaa tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga
     8581 aagaacttgc tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac
     8641 atgattttga aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa
     8701 cagttcaatc aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc
     8761 ttctattttt atttttttag catattaaaa aaataaccgt attatcgact gactacactg
     8821 atgtgctgct tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct
     8881 gccatgctga tcgtactgtc gccagcagcg tttctttgta aataatcatc acttaacaaa
     8941 taagtacctg tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa
     9001 tgtagatttt tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga
     9061 tagaagggtg catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag
     9121 aataacgaag aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca
     9181 atcaaatggg acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca
     9241 tcaacgacaa catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt
     9301 ataaacggaa catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa
     9361 tctctggaca atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt
     9421 gcaattttca taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat
     9481 tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca
     9541 ttgggtatag ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa
     9601 atgaaagagt ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga
     9661 cccttaactg ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg
     9721 ctgtttttct tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca
     9781 ggattttcta tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg
     9841 atcgtttttg ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc
     9901 ggcataacag ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat
     9961 actgtctgga caggtgctga agggatacta ggagcagtta caactgggct catcatcgga
    10021 tggggctatg cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct
    10081 gaaaatgttt ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg
    10141 cttatcggtt cagcggtttt tcaacagttg ttcagaatga ctgttgttca aggagtatgg
    10201 acatatgtcc agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta
    10261 ggtttttttg ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg
    10321 ttggttagtc ccgtcttgca agcgaattca ttagcgaatg cagaaatctt agcctcagga
    10381 caagagttga cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc
    10441 atgacggtta cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg
    10501 aaatcgaaga aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc
    10561 aatgaatcaa ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt
    10621 attttcgcac cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt
    10681 cctttattta cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta
    10741 gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt
    10801 tatttcccat ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa
    10861 aatgatggag cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag
    10921 attgggaaaa tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc
    10981 tttttggaca gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat
    11041 cgatgtccag atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa
    11101 gtagattagc agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc
    11161 tccgacccaa aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa
    11221 ctcttccatg atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat
    11281 catctggatg atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc
    11341 taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat
    11401 aacaatcttt ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg
    11461 cttaaaataa aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt
    11521 gatcaattca gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa
    11581 aattagacca tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct
    11641 ccttttaagg gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca
    11701 taatttcaat aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa
    11761 aaagagggag aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta
    11821 acagattaaa aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg
    11881 tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg
    11941 ctgctaaaga gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag
    12001 aaaacggtga aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat
    12061 acgccggtac agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata
    12121 tcatggcgat tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg
    12181 tgaatcaatc atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg
    12241 cggggtagat aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt
    12301 cgtactagaa aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa
    12361 agaaatcgaa ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc
    12421 atcaaaaaca aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc
    12481 aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg
    12541 cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt
    12601 tgattcaaaa gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg
    12661 acatttgatt gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga
    12721 atcaaaaggg atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta
    12781 cttatcacaa tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata
    12841 tatcttgatc acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca
    12901 aatcttgcaa caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt
    12961 gccaactctt gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc
    13021 tggtttcggc gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac
    13081 agtaattact gacgatctag gtttagaatt gaaagatgta acaatcgaaa acttaggaaa
    13141 tgcttctaaa gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa
    13201 agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga
    13261 ttttgaccgc gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt
    13321 taaagttggt gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc
    13381 attgaatgct acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct
    13441 agtgaacgtt atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat
    13501 caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta
    13561 tgaaggatca gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc
    13621 tgctactgga gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac
    13681 acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt
    13741 cgttgcagat aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat
    13801 gggcggtatg atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac
    13861 agataaaaag aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga
    13921 aattgctttt caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc
    13981 gctgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct
    14041 tttttgtgtg taattataat gaaaatagtt atttttaata gttataatat tttatctatt
    14101 aaaagtaaat atccattca
//
LOCUS       DASJSZ010000069        13779 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.070, whole genome shotgun sequence.
ACCESSION   DASJSZ010000069 DASJSZ010000000
VERSION     DASJSZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.070"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(35..2110)
                     /locus_tag="U4B03_002068"
     CDS             complement(35..2110)
                     /locus_tag="U4B03_002068"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN2068909.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2112..3029)
                     /gene="glyQ"
                     /locus_tag="U4B03_002069"
     CDS             complement(2112..3029)
                     /gene="glyQ"
                     /locus_tag="U4B03_002069"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEN2068910.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3413..4225)
                     /gene="recO"
                     /locus_tag="U4B03_002070"
     CDS             complement(3413..4225)
                     /gene="recO"
                     /locus_tag="U4B03_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEN2068911.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4368..5267)
                     /gene="era"
                     /locus_tag="U4B03_002071"
     CDS             complement(4368..5267)
                     /gene="era"
                     /locus_tag="U4B03_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEN2068912.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5282..5677)
                     /locus_tag="U4B03_002072"
     CDS             complement(5282..5677)
                     /locus_tag="U4B03_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEN2068913.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5661..6137)
                     /gene="ybeY"
                     /locus_tag="U4B03_002073"
     CDS             complement(5661..6137)
                     /gene="ybeY"
                     /locus_tag="U4B03_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN2068914.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6154..8346)
                     /locus_tag="U4B03_002074"
     CDS             complement(6154..8346)
                     /locus_tag="U4B03_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEN2068915.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8370..9341)
                     /locus_tag="U4B03_002075"
     CDS             complement(8370..9341)
                     /locus_tag="U4B03_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN2068916.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10047..11426
                     /gene="brnQ"
                     /locus_tag="U4B03_002076"
     CDS             10047..11426
                     /gene="brnQ"
                     /locus_tag="U4B03_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN2068917.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11580..12026)
                     /locus_tag="U4B03_002077"
     CDS             complement(11580..12026)
                     /locus_tag="U4B03_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEN2068918.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12053..12229)
                     /locus_tag="U4B03_002078"
     CDS             complement(12053..12229)
                     /locus_tag="U4B03_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEN2068919.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12391..12819)
                     /locus_tag="U4B03_002079"
     CDS             complement(12391..12819)
                     /locus_tag="U4B03_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2068920.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12911..13771
                     /locus_tag="U4B03_002080"
     CDS             12911..13771
                     /locus_tag="U4B03_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEN2068921.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT         3766 a         2870 c         2491 g         4652 t
ORIGIN      
        1 gtgcctgttc tctggtttat ttacatatta gaaattattt tgtgatgatt tgatctaaac
       61 ttgctagtga caaagccatg gcagcaattt gacttaattg cgttaatcgg ttcttacgta
      121 ctgctggttc ttctgccatg accatcgtct gatcaaaata attttcgatc agtgggcgaa
      181 ggcttacaag ctgatcataa ttttcgttga tcgtttggtc agcaaagctt gttttcattg
      241 cctctactgc tttgtgcagt tctttctccg cttcgttttc gaacagttca gggtctactt
      301 ctgattgttt ttcaagctcc gcttttttcg ctaagttcac gacacgtgtc aaagcttcca
      361 tcgatggttt gaagtcttca tctgcaagat gctgcttgaa caattgtgct gctgcaaata
      421 cttttgtcag atctttttgt tctgcattca aaacagcttc gatgacatca tgacggatat
      481 tcttcgttgt caataattgg cgcagacggc ctttgataaa gtcgatcacc tgttgttgtc
      541 ccgctgacaa gccaatgcca aaacgatccg tatctttact gataacttca ctaatcgttt
      601 cttgcagaac agacaaagga aaggcccatt ttttcgattc gatgattcga acgacaccat
      661 atgcttgacg gcgcaaagca tatggatcgt ttgagccact tgggatcatc ccaactgcaa
      721 agaaagaaaa gatactatct aatttatcag caatcgctaa aacagaacct actgtagtct
      781 caggtaaatc tccttcactg gatataggca tgtaatgttc acggatcgct gttgctacgg
      841 ctggtttctc gcctttaagc agagcatatt tttcgcccat gatcccttgt agttcaggga
      901 attctcctac catgttggtt actagatcaa atttatagat ttcagcagcg cgttgcagat
      961 ccatcaattc ttcctcagat agtcctactt gttcgcctat gatgcgagcg atgacttgta
     1021 cccgttgcat tttctcatag atgctaccga ttttttcatg gaaagtaaca gtttttaatt
     1081 tttcaacgta ttggtcgatt gaagctttct tgtcttcatt ataaaagaat tccgcatctt
     1141 ctaaacgagc agtcaatact ttttgattgc ctcgtaccac attttccaat tttacccgat
     1201 cgccattacg agcagaaata aaatgaggaa gcaacatacc ttgatcgttc cgtacttcga
     1261 aataacgttg atgttctttc atagaagtga ctagtacttc ttcaggaaca gatagatatt
     1321 tttcttcgaa gtcgccagaa aagacagttg gatattcgac taaattgttg acttcttcta
     1381 gtaaatcttc atctagatca atgatccagt gatttttttc tgctaattcg tttgcttgtg
     1441 tgatgatcat ttcttttctt ttttgcggtg aagcaataac aaattgttca ttcaattttt
     1501 cttcgtattg ttttgcatgc gtaaatgtca catcttcgcc taagaaacga tgacctcttg
     1561 aagctctgtc tgttttgaca tccaagatct caaacgggat tatttcgtca tctaacaatg
     1621 ccacgatcca gtgaataggg cgaatatatt caaaatcgta ttctgcccag tgcatcgtta
     1681 ctgggaaagt gaggctttta atgacatctg ctaaacctgt caatacttct tttgcttctt
     1741 tgccgtgagt gtatttcgtt acatagacat attccacgcc ttttaattct ctaaaagtga
     1801 tagcatctgt cgtcagacct tgtccgcgga caaatccttc agctgctttc gtccagtttc
     1861 catctgcgtc caaagcaatt tttttggctg gtcctttgac ttcttcttcg ctgtctgctt
     1921 gacgttctgg aatgtttgtt actttgattg ctaagcggcg cggtgtagaa aacgtttcga
     1981 tcgtatcata agtcaattgg ttttcatcta aaaattttgc tgttttttct tctaattgtt
     2041 tcatgcttgg tgtgactaca tgagcaggta tttcttccag cccgatttca agtaataagt
     2101 cttttgccat cttattcagc ctccttcacg ttttctggat ttgctgtgtt tttattcaat
     2161 aaagggaatc ctaatttttc gcgttctgcc acaaagattt tggctacagc acgagccatg
     2221 ttgcggatac gggaaagata acctgcacgc tctgttacgg atactgcacc gcgagcatct
     2281 aataaattga atgtatggct acatttcaaa atataatcat acgctggatg aacaagattt
     2341 tcatcgatac agcgtttagc ttctttttca aaacgatcaa aattgcttag tagcaggtct
     2401 tggtcgctta cttcaaaaga atatttagaa tgttcatatt ctggttgttt gaagatctca
     2461 ccatatttca cgccatcagc ccattccaga tcataaacac tttcaacttc ttggatataa
     2521 gaagcaagac gttctagtcc ataagtgatc tcagaagtaa ctggtttaca aggtagtcct
     2581 ccgacttgct ggaagtatgt gaattgtgtg atctccatcc catcaagcca tacttcccag
     2641 cccacaccgg cacaacccat cgatgggttc tcccagttgt cttcaacaaa gcgaatatcg
     2701 tgttctaatg gatcaattcc taaaagacgc aagctttcta aataaagctc ttggatattt
     2761 tcaggtgaag gtttcattac gacttgaaat tgatgatgct gatacaaacg gtttgggttt
     2821 tctccataac gcccgtcagc tggtcggcgg gatggctcta cataagcagc attccatggt
     2881 tccggtccga ttgcccgtaa gaatgtatat gggctcatcg tacctgctcc tttttctgta
     2941 tcataagctt gcatcagcat gcagccgttt gatgaccaaa atttttgtaa tgttaaaatc
     3001 atatcttgga cggtcaattt tttactcatc tgttttgccc ctttcaattt ttaagcgttg
     3061 ctgtcaataa gaatacagga aagaaaacga caaaaagtcc ctatgtctga ttacagacat
     3121 agggacgaat aatttcgcgg ttccacccta cttccgaata aagattcggc agcttcattt
     3181 atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc ggcttccact ctcccgaact
     3241 cgctaaacca cgtgtttttt actgatcttg gtcaatgtac gtattcattt tgcagttttc
     3301 tggtttgtat aatagatcct ctcagtgaac gatctattta atctacggtt aataatagac
     3361 ggaagtccct gtatttgtca aatagttttg atttttttgt tgtatctttc atttattctt
     3421 ctttttcagg tatctctttt tcaggcatat ttttctctgg cgtctttaat acgtgtgccc
     3481 aagacttcat ttgatcaatg aactttttgc tttttagatg aataccgaca tattcatcat
     3541 acaactggtc aatgaggtca cggattgcct gcttcgtttc ttctttcaag tggattccac
     3601 tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa ataaacagcg cgaggatctg
     3661 catgataacg gtgttcgtcc attggccagt gtttctcgca tatcacaccg ccatatttgg
     3721 aagaatagtc aaattttcct tttgtttccc cgcatacgac acagtgcgtc caaattggcg
     3781 cgattccgaa gcgttgcagt atttgcactt caaagatatt cgtgatgatt tctgcatcat
     3841 ctccttggtc catccgctgc agagcttgtt gcgtaaattg gaataaatgc gggtcatatt
     3901 gatgatcttc gattgctgca tctactagat ttagcaaata cgtaccataa ccgctcaaaa
     3961 agatatcttc ttggatgttc cgaaacgcat ggacgtcttt gacactgctt aaaaacgaca
     4021 aaccttcact tcgaaaatta ccgatataga ctgcttcggt aaaggggagc aaagccggag
     4081 ctaatggctg attttgccga tggattcctt tgatataaaa catcattttg ccagaagatt
     4141 ccgtaaagat tttgactaat ttatcttttt ctttgtaatt acggctagat aaaatcaagc
     4201 ctttcgattc tgcaagctgg ctcatattct ccctcctaaa aaataaatct ggaaaagcag
     4261 attctgccga tccagattat ttatgtttcg atcttccgct gtttttctca acgtacggat
     4321 ttttatccag tttatttcta tacctaacct ataagaagtc cagaatatca gtactcgtct
     4381 tttcggtagc cgaagtcttg cagatagact tttttgtctc gccagtcttt ttggactttg
     4441 acccaaagtt ctagaaatac tttgtctcct aaaagatttt cgatatcctt tcttgctttc
     4501 gttcctactt gcttcagcat ttttccgcct ttgccgataa tgattccttt ttggctatct
     4561 ctttcgacga tgatcgtggc ctggatatgg attttatcat tctcgtttcg cttcatcgaa
     4621 tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg ttaaaaacaa gactttctca
     4681 cggatcaatt cagaaacgat gaagtattca ggatgatcag tcacttgatc atctggaaaa
     4741 tattgtgggc cctctggcat ttcactgact aatacatcca ttaagcgttc gacattgttt
     4801 ccttctgtag cagagatcgg aatcacttgt gcaaaaggca tctgcgtcga ataatcttcg
     4861 atgattccca gcaactcgtc tggatggatc ttatcgattt tattgataat cagataaaca
     4921 ggagaatgca cgttttttag acgttcaata ataaaatcgt ctccccggcc acgtttttga
     4981 tcagcactga tcataaataa gactgcatcc acttctttca acgcactgta agctgcttct
     5041 accataaaat ctcctagacg atgctttggt ttatggattc ctggtgtatc gataaaaatc
     5101 agctgagctt ctggtgtagt atatactcct tggattttat ttctagttgt ctgtgccttg
     5161 tcactcataa tggcaatttt ttgacccacg attcggttca ataatgtgga ttttcctaca
     5221 ttcgggcggc cgacgattgc gacaaaccct gatttatgtt ctggcatata cgttctcctt
     5281 tttaattaaa caacaaatga tatattttag ggacaaataa aatcaaccca atgatgacag
     5341 caaaacaagc ggtcactaaa actgctccag ctgccatatc tttgattttt ttcccaatcg
     5401 gatgaaaatg aaaatccgta aacatgtcca ctacattttc aaaaacagta ttgatgatct
     5461 ctactatcca aacgagaaaa acagctaaaa gcagccataa ccactctatc cgatcaagct
     5521 ggaaaatcac gcccataaat aaagcaagga taccgaacgc aacatgtttc ctcatattgc
     5581 gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa ttccacagac atcaggaaat
     5641 gtttgttttt ctctacgttt ttatcttttg agtccatagg catccaagat ctctttctgt
     5701 aaaccaaaca tttctttctc atcttctggt gtcatatggt catacccgtt gatatgcaag
     5761 aatccatgta ccgctaaaaa gccaagttcc cgatcatatg aatggccgta agcttctgct
     5821 tgttcagcag cacgttcaat agagatcatg atgtctccta gtacacgagg taatggattc
     5881 tctccatctt caaaaatcac tgggatttcg tcttcgcctt cttcttccag agcaaaactg
     5941 atgacgtctg tcggtgcatc ttttccacgg taatcacgat tgattacttg gattgccgca
     6001 ttgtccatga aagtaacaga catttctgtt tcctctggca gctttaaata agatgctgca
     6061 aattgcagca agtcatcgat ttctttcatt ttctcttctg agacttggtt tgtttcatcc
     6121 ataaaggtaa tatccattat acatcgcttc tttctattct tcttgttctt cctttatctt
     6181 ctcaacttct gttttcattt taggatattt gatccgagaa tggaaggtgc tacttaaacc
     6241 gctgattaat gatttttcca cttttcggat ttctttcaag gtcagtccac tatcgtctaa
     6301 ttgaccatcg gaaatacgct cttcgataag attatggaca aaactttcga ttttttcgct
     6361 cgtcggatga tccattgccc gtactgcagc ctcacaactg tcagcaatat tgacgatccc
     6421 tgcttcacga ctttgcggtt ttggtccagg ataacggaat tgctcttctg ttacttccgg
     6481 attgcgttct ttcgctttga tgtagaaata acgcattagc gtggttccat gatgctgttt
     6541 gcaaatatcg atcaccattt gcggcatatt ataatcttcc aatattttgg ctccatctgt
     6601 cacatgtcca aagataatat gtttactgtc ttctggcaat aagaaattgt gcgggttttc
     6661 cgctcctgaa ggcaagtttt caacgaaaaa gctagcatgt ttgatcttgc cgatatcatg
     6721 ataatagcaa gctactcgag ttagcaaaga acgtccgccg atttctgcta cagcgttagc
     6781 acttagatta gctaccatca tactatgatg ataagtacct ggcgcttctt ccagcaactg
     6841 tttcaataaa ggatgatttg gattgctgag ttcatttaat acaattacac tgtcatcatt
     6901 gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc aagaatgaga agagtgcacc
     6961 agtgaacccg cagacgagca ttaaccaagt cgttgcatca ccaaagctca ttccttgata
     7021 gacgatcaaa atgacatcca tcatcacagg aaaaataatg acccacatgg cagcagataa
     7081 tccttgttct gagatccgtt tgcgtctgac aatcgttgcc atgagcccag agaaaacata
     7141 agacatcaag ataatcgtta gaaagttcgt gccaatcgaa tcataaaaaa tgaaaagtgc
     7201 ggatactgct tggaaaatac cagccatgat ccctgctctt cgattcaaga aaaagcttaa
     7261 aacaagtggt gcaaaagcag cgggataaaa cagcgggata taagtagctt gttccgtttg
     7321 gaataattgg aaaaatttca tcagcactat gctgactgac atagctgtca catagaaaag
     7381 cacgtatttt gttctttctc ctgcattttg gaattgaagt gaattataaa tcagtacgcc
     7441 gatttgaagc aaaacagcta aaaccatggc aattagcgga aaaatcgaag ttgtttgatt
     7501 agtcagtcct agtaattcca attttttcat agctgaagca tcgatttgat taccttcacg
     7561 aacaatgatt tctccttgga agatcatcac tggttgtaca gcttctcgtg cggattgttt
     7621 caattcttct gtctttttct cattcaatac gtcattcact acgatacctt gatccactaa
     7681 gtagcgaatc atttgttgtt gtgctggtgt gacattcaaa tattggattt gctcttctgc
     7741 ttcttgttta aaagcttcca attcactttc acgaacttgt ttgctcatct gttcgtcaat
     7801 gagcttcaga ctctcatccc tcacagtatg aatctcagtt tcagtcattt caaatatcgt
     7861 cgaataaaaa ttgttcggta gtttttgata aaacacaaca ttttctgcat tcacattttc
     7921 aaaatttttc ttcagcgcgg cgatccgctc ttctgtagta ggctttggga tattttcatc
     7981 ttcctttgcc ttatcaatct tttcgtcata agatttgttg atggcatcat tcgtcttatc
     8041 gatcaattca aatagttgct tgatccgatt gttctggtca tctgcaagat ctttttggta
     8101 agtgtattct ggagtgactg cttctgctgc cagttttctt ttttgttcgg tttcttccgt
     8161 gttctcgatc gttttgttcg cacggatact ttcttctgct acttggcctt ctctgaaatc
     8221 attggccttt tgtttgacac tactaaatgt aacgccgcag ataatgataa aaaacaagaa
     8281 cagcaggaaa ggcaatgctg cctttccaag tttttcttgt agtttctgca gtgttttatt
     8341 caacatgtgc ttatcctcct aagcaaagct tattcttgat ggagatctgc cttttcataa
     8401 gctttgatga tttcggcaac tacagggtgc cgaacaacgt cacttgcttc aaaattaaca
     8461 aaatcgattt tcttgatttg ctttaatgtt cgttctgcat gaacaagacc acttgtcgtc
     8521 cctcgaggca agtctatctg acttgtatcc ccattgacaa tcatttttga ttgataaccg
     8581 agacgggtca agaacatttt catctgagcg actgtcgtgt tttgtgcttc gtccaagata
     8641 acgaatgcat catccaatgt tcgcccccgc atgtaagcta aaggtgcgat ttcgattacg
     8701 cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa tttggtataa agcatcataa
     8761 acaggccgca gataaggatc cactttttct tttagatccc cgggaagaaa gcctagattt
     8821 tcacctgctt ccacagcagg ccgcgtcaaa atgatttttt gcacttcacc ttttttcaag
     8881 gcagcgattg ctaatactac tgcaagaaaa gtttttcctg taccggctgg tcctacgcca
     8941 aatacgatat catgtttggc taccgattcg acatatttct tttggccgct gttttttacc
     9001 cgtataggtt ttccattacg gtctttgacg atctcttcct catacatctc aacaaaataa
     9061 tctaatgtac ctttttgccc cattttcaat gccgtcacaa catcgggcgt acttacgtgg
     9121 attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg aggcagccaa ggaaatctgg
     9181 gattgctcac caatgatctg aatgatttct ccacgtgtat ggatggttgt ttccgttgtt
     9241 tcctcaatca gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac atcatcagca
     9301 gccgtcaatc taatctctaa agaactagac tcttccgtca aaaatagaca tccccttttt
     9361 ttcgattcat tctttcctat tttaccatac tcagaagaaa ctatatagca aataagaaaa
     9421 ggttctaaaa gagttgctta accataagta ccaagaaacg ttaaaaaact agccgcttcc
     9481 attctctaaa ggtctcgttt gaataaaaaa agctgatcat gtaagctatg cataagatag
     9541 aggacaagta ttccgtctaa cttagcaagt attctctggg tatttttctt gtttcaaaaa
     9601 aagaaaacaa aatatttgaa atattcttgc atttcaaaaa aaacaggtgt attataacgt
     9661 caacaaataa atatacagca aagcgtcgag gaaaacagta ggctattcag tgatttattc
     9721 ccagtgagtc atgtaagtga gaaatgacaa taaatcgaat agacgaacat catttccgag
     9781 tcaatgtcga aaacgtagat cattgtggct gcctccatta tcaggctagc ggataacttt
     9841 atcgaaaagc gtccgtgaat gaagaagctt ttttaagcga aaaaaggtgg taccgttcta
     9901 gaatctagag cccttttccc aagtggaaaa gggctttttt tatattttaa ggtggtggtt
     9961 ttcatggaat ctgatattag tggtatttct agtttctgca acgtttattt tgttactcaa
    10021 aatgataatt attggaggaa aaatttatgg aagaaacaaa aacttattca tggaagcaat
    10081 tattgatggt cagttcactt atcttcggga tgttcttcgg gtcaggtaat ctgatctttc
    10141 ctgtacactt aggtcaaatg gctggatcaa actggatcag cgctggtgta ggatttgcca
    10201 tctcaggcgc tcttttccca ttattagcta tcttagctgt agtagtgaca aaaagtgatg
    10261 gtctgtatga cttagctaaa cctgtcggaa aatggtatgc tgccttgttc ttgatccttg
    10321 ttcatttaac gatcggacca ttctttggta caccgcggac agcagcaact gcttttgaaa
    10381 tggctgctaa accctttttg cctgaaaaat atgattcatt aggtatgttt ttattctctg
    10441 ctcttttctt tttacttgct tatttcctta ctgtacatcc aacacgtttg gtcaaatatg
    10501 tcggaaagta tttaaactcc ttgtttcttg cattattagc tatcattttt gtcgtttctt
    10561 tattccaacc catggggaat ctaaaccaat ctgctgcaga ttcttatcag acgaacgctt
    10621 tgacaaatgg cgttttagaa ggatacaaca cggttgatgc agtagcttta cttgctttaa
    10681 gtgtcacttt tgtccatgca gttcgtgatc ttggttttaa agataaaaaa gtcacagaat
    10741 taacagcaaa agctggtagc ctgagcgttg cgttagaagt catgatttat tttggtctgg
    10801 tccttctcgg tgcgatgagt ttgaataaga tgtctttatc agaaaatggc ggaactgcac
    10861 tttctcagat cactaatttt tatttaggta acttcggtgc tagcttctta ggggtaatgg
    10921 tcacattagg tgtcttcact actgctatgg gactcgttgt ttcttttgca caagatttcc
    10981 ataaactgtt ccctaaagtc agttatatga catggctgcg gttaacgact tttgtctctt
    11041 ttgtcgttgc gaatgccgga ttggataata tcatccaatg gtctttacca gtattgatgc
    11101 ttttgtatcc attgtcactt gcgttaatct tactttcctt aacagcaaaa ttcttccaaa
    11161 agagtccatt tgtctatcaa gtaacgatgt tcttttctgc cgttccagcc gtattggata
    11221 tgttggcaaa ttcgcctgca ttagtgagtc agcaaactgt cgtagcaagc ttactaggat
    11281 tttatcatca ttatgttcct tttgctgcat taggtcttgg ctgggtcgtc ccaacgttgc
    11341 ttggttacgc tggcagcttg ctcttttatt atgtatatcg cgtatcagga tataaacaag
    11401 aagcaagtga gttaccagaa gaataaccaa atcaaacaca atttaacata agagatatta
    11461 aacaggaaaa aaacagctag aagaaagaat aagattcttt cttctagctg ttttttagtt
    11521 ctttttttac gaacacaaaa aactgcggat aatcactgtc cgcagtttaa atgataaaat
    11581 taagattgta atagttcttt cacaatcgca ttcacttggt ttccatctgc ttttcctttg
    11641 actttaggca taattgcacc cataactttg ccaaattctt tcattgatga agcaccagtt
    11701 tgagtgattg cttcttgaac aagttgtcga atttcttcgt cggaaagttg ttccggcata
    11761 tatttttcga ctattacaat ttcacttttt actttttcag ctaaatcgtc acgtcctgct
    11821 tcttcaaact catgcaaaga atcacgtcgc tgtttcattt cacgagacaa caccgttagt
    11881 tcttcttctt ctgtcaagtc gtgaccttct ttaatctgct cgttttgtat agatgacttg
    11941 atcattcgga tgacttgtaa agattcctta tctttcgctt tcatcgctgc tttcatgtca
    12001 tcgtttaatc tacttagtag tgacacacta ccaactccaa ttcatcacag aactagaatt
    12061 ttttgcgttt tctagctgct tcagattttt tcttgcgtct tacacttggt ttttcgtaga
    12121 attcacgttt acgagattct tgtaaagtac ccgcttttga cacggaacgt ttgaagcgac
    12181 gaagagcatc gtcaagtgat tcgtttttgc gaacgactgt ttttgacatg ttaattccct
    12241 ccctccgagc ttaaaaagta accgtaattg ttattgaaat atatctcaaa aaacaagact
    12301 gtcctgtaaa tattatatct aatgctgttt tgaccgtcaa ggaaatcttg ctttttttta
    12361 aaattatcaa atgttatttt atttaaatcc ttattgacaa ttttcacatc ttccgaaaat
    12421 ctcgaatcta tgcccttcga tcgtgcatcc acctagctgt tcttcgaaat aattcatcgg
    12481 acacatatgg atttctttcg ttgttccgca cactgtacag ataaaatgat ggtgatggcc
    12541 gattttcccc tgacagcaat gaaaacgaaa cttcatttct ccatttaaat cggtttcttc
    12601 cagtaaatcg atctctgcaa aatcatggag attccggtaa atcgtatcat aacttacccc
    12661 tttgaattcc tgattcatat agtcaaaaac ttctttagca gatacgtagc ggttacgctt
    12721 cactaaataa gttaacaatg cttctctttt tttagtatat ttaaaaccgt tatttttcaa
    12781 tgtttctaat gattgttcga tcaatgtctg ttttttcatg acttattcct ccaaatcaaa
    12841 atgattacga agtacgcatt ctagagtatg gtataataaa cataaaaaaa atgcaagtga
    12901 ggttttatcc atgtcttccg attctacaga aatcgttact ttttttgtca tctccgactc
    12961 tgctggagaa actgcgacaa aactagcgca agctactatg gctcagtatc ctaccgttga
    13021 gttcaaccta ttccgcagaa cgttcgtgac agatgaaaaa acattaaggc aggcattgca
    13081 agatgcatta gaagaaaaag cacttgtcct gcatacatta atcaatcaag aactgatcga
    13141 gataacacgt tctttttgtg aagaacatca attatttagt tttgatgtga tgacgccacc
    13201 gatatcagaa attgaacgtt taactggtat caagccaatt cggcaacctg gtgccctcca
    13261 tttattgaat gagaactatt tcaaacggat caaagcaatg gaatttgctg taaaatacga
    13321 tgatgggaaa gatcctcgtg gttttttaga agcagatgtc gttttgctag gcgtatcccg
    13381 aacttccaaa acgcctttga gcttgttttt agctaacaaa aatttaaaag ttgccaattt
    13441 acctttgatc ccacaagcac atattcccaa acagctttgg gagatcgatc ctaaaaaaat
    13501 cgtgggacta acgaataacc cagatatttt aaataatatc cgtaaagaac ggatgcgctc
    13561 ttacggatta aaccctgata ctgcttattc cgatatcgaa aaaattagag ctgaacttga
    13621 ttttgcgaat gatctttatg aaaaactagg atgtgtagtg atcaatgtgg catctttatc
    13681 tattgaagaa acggcttcgc ttattttgaa tgctttagac ctagaagatc atagttatta
    13741 tggaactgaa acaacagaaa aaagcgatta atcattttt
//
LOCUS       DASJSZ010000070        13298 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.071, whole genome shotgun sequence.
ACCESSION   DASJSZ010000070 DASJSZ010000000
VERSION     DASJSZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.071"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..2376
                     /locus_tag="U4B03_002081"
     CDS             4..2376
                     /locus_tag="U4B03_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068922.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="U4B03_002082"
     CDS             2437..3897
                     /locus_tag="U4B03_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN2068923.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="U4B03_002083"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="U4B03_002083"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2068924.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="U4B03_002084"
     CDS             6128..6277
                     /locus_tag="U4B03_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068925.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="U4B03_002085"
     CDS             6352..6945
                     /locus_tag="U4B03_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEN2068926.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="U4B03_002086"
     CDS             6958..7857
                     /locus_tag="U4B03_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN2068927.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="U4B03_002087"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="U4B03_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN2068928.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="U4B03_002088"
     CDS             8723..8983
                     /locus_tag="U4B03_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068929.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="U4B03_002089"
     CDS             9428..9634
                     /locus_tag="U4B03_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEN2068930.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9682..9885
                     /locus_tag="U4B03_002090"
     CDS             9682..9885
                     /locus_tag="U4B03_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068931.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="U4B03_002091"
     CDS             9901..10338
                     /locus_tag="U4B03_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068932.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="U4B03_002092"
     CDS             10331..11038
                     /locus_tag="U4B03_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068933.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11418..11597
                     /locus_tag="U4B03_002093"
     CDS             11418..11597
                     /locus_tag="U4B03_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068934.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="U4B03_002094"
     CDS             11922..12185
                     /locus_tag="U4B03_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEN2068935.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="U4B03_002095"
     CDS             12179..12529
                     /locus_tag="U4B03_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2068936.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="U4B03_002096"
     CDS             complement(12553..13167)
                     /locus_tag="U4B03_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2068937.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4921 a         2198 c         2735 g         3444 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaa
//
LOCUS       DASJSZ010000071        13287 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.072, whole genome shotgun sequence.
ACCESSION   DASJSZ010000071 DASJSZ010000000
VERSION     DASJSZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.072"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(602..1786)
                     /locus_tag="U4B03_002097"
     CDS             complement(602..1786)
                     /locus_tag="U4B03_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEN2068938.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1811..2818)
                     /locus_tag="U4B03_002098"
     CDS             complement(1811..2818)
                     /locus_tag="U4B03_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2068939.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2990..3349)
                     /locus_tag="U4B03_002099"
     CDS             complement(2990..3349)
                     /locus_tag="U4B03_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068940.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3321..3692)
                     /locus_tag="U4B03_002100"
     CDS             complement(3321..3692)
                     /locus_tag="U4B03_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEN2068941.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3709..4011)
                     /locus_tag="U4B03_002101"
     CDS             complement(3709..4011)
                     /locus_tag="U4B03_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068942.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(4028..4498)
                     /locus_tag="U4B03_002102"
     CDS             complement(4028..4498)
                     /locus_tag="U4B03_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEN2068943.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4473..4751)
                     /locus_tag="U4B03_002103"
     CDS             complement(4473..4751)
                     /locus_tag="U4B03_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN2068944.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4748..5797)
                     /locus_tag="U4B03_002104"
     CDS             complement(4748..5797)
                     /locus_tag="U4B03_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEN2068945.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5754..6683)
                     /gene="tadA"
                     /locus_tag="U4B03_002105"
     CDS             complement(5754..6683)
                     /gene="tadA"
                     /locus_tag="U4B03_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEN2068946.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6815..8569
                     /gene="ade"
                     /locus_tag="U4B03_002106"
     CDS             6815..8569
                     /gene="ade"
                     /locus_tag="U4B03_002106"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEN2068947.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8667..9329)
                     /gene="trmB"
                     /locus_tag="U4B03_002107"
     CDS             complement(8667..9329)
                     /gene="trmB"
                     /locus_tag="U4B03_002107"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEN2068948.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9399..10181)
                     /locus_tag="U4B03_002108"
     CDS             complement(9399..10181)
                     /locus_tag="U4B03_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEN2068949.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10385..11596)
                     /locus_tag="U4B03_002109"
     CDS             complement(10385..11596)
                     /locus_tag="U4B03_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2068950.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11589..12326)
                     /locus_tag="U4B03_002110"
     CDS             complement(11589..12326)
                     /locus_tag="U4B03_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2068951.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12464..12898
                     /locus_tag="U4B03_002111"
     CDS             12464..12898
                     /locus_tag="U4B03_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEN2068952.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12891..13235
                     /locus_tag="U4B03_002112"
     CDS             12891..13235
                     /locus_tag="U4B03_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN2068953.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
BASE COUNT         4119 a         2758 c         2233 g         4177 t
ORIGIN      
        1 cgagtaattt tctagtatga ttatccattg agagactcct ttggtagggt gacgttttgg
       61 tcgactaaat tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaaagtgtta
      121 gtagaaaatc gctttcgatt ttccaccaac acaaaatatt atagaaccgt tttttcgcaa
      181 caaaaaactt gattttcata cctgtgcttt cacgattcag gcaaagtaca ggtggaaaat
      241 caagttttta ttattcgatc aatgaatgtt tcggatcggt tttctagcta aacataaagc
      301 gaagaataaa gtcgacagcc cacgaggctg atctaaaaat gttttttctt tttacatatg
      361 taatgtccct tataaagatg aatgaaaata aaatattggg attttcctga aaatcaataa
      421 ataaaatgcc agtcttttcc cttttgacca cttttccaag caaaattccc taagaagatc
      481 ctgtaaaaaa gaagggaaat tgaagttaaa ttaccatttt gcggtcttct ccgcaaaaaa
      541 aacacatgca agaattccct cttgcatgcg tttcatcttt tatcctaacg aatgctttgg
      601 cttagccgcg cagacgctcc acatcacggg caatcattag ttcctcatct gttgggatca
      661 ctaatactct tacttttgat tcttttgtag agatatctac ttcttcacca cgcaattcat
      721 tcaacgctgg atcgatttca caaccaaacc aagacatacc tttgatgatt tcactacgta
      781 catgtccgtc attctcgccg ataccagcag taaagacaat tgcatcaaca ccattcatcg
      841 ttgtcacata accgccgatg tattttcgga tgcggtccac gaaaatgtca taagctaaac
      901 gaatatgttc tttttcatag ttcttttcaa gatcacgcat atcactagac aatcctgtta
      961 ggcctagcag accagatttc ttgttcaaaa cattgatcat ttcatcaata tcaatctcta
     1021 atttttccat taaatatggg agaacagctg gatcgatatc acctgaacgt gtacccattg
     1081 tcaccccagc taatggtgtg aatcccatag aagtatccat ggatttacct ccatcgactg
     1141 ctgtgatcga tacaccattt cctaagtggc aagtgatgat ttttagttct tcgatcggtt
     1201 tgttcaataa ttttgccgca cgttccgaca cataacgatg acttgtgcca tggaagccat
     1261 attttcttac tttgaaatct tcataataag acaccggtaa gctatataaa taattttgct
     1321 ttggcattgt tgagtggaaa gatgtatcga atacagccac actcaaaatg tcaggtaaaa
     1381 tctttttaaa cgctttgatc cccattaagt ttgcaggatt atgcaaagga gcaaattctg
     1441 ctaattcagc aatgcggtcc attactttct catcgatcac tactgaatcc ccataagttt
     1501 ctcctccgtg aacaacacgg tgccctactc cggtgatttc atcataagag cttaaaattt
     1561 gcagttcaat caactgatct aacagcattt ttactgctac ttcgtggttt tcgatatcca
     1621 tgatttgttc gaatttttga ttatcgccgt acttgatggt aaaaatagaa tcttgtaaac
     1681 cgatacgttc gacgatccct ttcgcaacta cggtttcttc tggcatttga tataattgcc
     1741 atttcaaact tgaactccca gcattgattg caattgtttt agacatatga ctctccttct
     1801 cgttgaatga ttacagagca gatgatttcc agtttttaaa ctcgttgaaa aattccgtca
     1861 ccttctctgg ttcttttaat gaagcaagtt tcacaagcaa gacttctttt acttgttgtg
     1921 cttgtggtcc ttttttctgt aagatcaaaa tacttttttg cgattgttta ttacggaaca
     1981 attcatctgg caattgaatc attccttgaa gataaccttc ttctttaaac cattttttga
     2041 tttcttcact ttgatccgtt tctaaaaatc cactcggcat caaaaacaaa ccaaaaccat
     2101 ccggcttcac atatttcatt gattgctcta gcaataaatg atgtgcataa ctatggcctt
     2161 cttcggtact tgttaaaaac tcttttgctt tttcgtcatt cggataataa ccgattggca
     2221 aatcgctgat tgcaaaatcg acaggatcta ttaataattc ctgcaacccg tcttgatgaa
     2281 agtattgaac atttgcttgt gtcaaatcac ttgtagaagc tgcgacagcc aacaatgtat
     2341 catcaatatc cacaccaatt ccttctgttt ggtaccccgc attcgatagg tttaaaagaa
     2401 ctgttaataa caagttgccc attcctgccg caatatccag aatcttaacc ggtgcttttt
     2461 tatttgtgta tagttgttca ataagaaaaa caaataaaaa accgatacta tcaggagtga
     2521 gttgatggtt cgcctgcaaa tgttctgtct ggctgccctt caataaaagt aattgagaca
     2581 gtctgcgcca ttcttctggc tcgaaagaaa gtttcttcaa ctcttcataa agagctgtga
     2641 tccgttgcgt tgtctctttt gtcggcactc catccaccac acgtacttga taatcatcta
     2701 ttaaattttc tgcattttcg acataggctt ctaaaaagga agttcccaat gcattctgta
     2761 aaagctgaat agcttccagg ttttgattaa acccttgttc cattttttct ggtaacatgg
     2821 tgcacctcat ttctagatat attataacaa ttttttcacc tgtttaattc tatcgaacaa
     2881 ttgacaaaat ttcaatcgtt ttcactgttt tttatttgtg aaacggcaaa cattttttta
     2941 ttaaaaacag tgttataaca gaaaagaggg cgttttcacg aacattccat cattctgtac
     3001 tagtatctgt atcatttttt acttcttctg tgaaccgaaa gataaacggt gagacttcca
     3061 cctctaagaa tagggactgt tgtacccact gatatgttac agatccagtt gtgtagtaaa
     3121 aaactccttc cgaactttcg gcaagcgtag actgttccga taaagccatc tgcttcatta
     3181 tttttgctat ataataattt ttgattcgta tattaagatc cattgacaaa cggtagcttt
     3241 ctaatgctgc catcaataaa aacgtacata aaagaaataa aagcaagaca gaaaataaaa
     3301 tagagccctt ataacatttt ctactgatcg ttttttgtcc atatggcata tccatcttct
     3361 ccattttcaa agtgtacata aaaagaaatt gtgtgttctt tttccaataa ttgaagctca
     3421 gtgatacttg ttaacatagg ttgatgtccg ccggaagttc ttcttttacg tatcattgcc
     3481 tgatatcttt cgatgatata ctgtttatcc tcgatcgtgt agtgcagttg ctgatttttc
     3541 acagagacat tgtgactgct cttgagttct tcttctaatt gtgctaaaaa tattagccac
     3601 tctttttgat ctttacgctg caaaaattca cttacttgga tggtttgctg aatcaaaaga
     3661 ttgaatgtga gcaatagact gctcaatatc atcagtccaa ttagacattc aatcagcgta
     3721 aatgctggaa ttacttgttt tttcaatact gatcgcttct ttctccatat tgacaaacac
     3781 tttttcgatt actccattat tatcgacaat ttggatatcg tatgggatca tttcttccct
     3841 tcgctctggt cttttgtgat acatccgctg cgtcttgata tcttcataaa tcattcgaat
     3901 aaatagcagt tcctccccac ttttcttttc tgcctgaagc aaaaacagct ggccaaagct
     3961 cactaagtag acacagacag aagcgatcat aagcgctgta agggactcca acaaaacaaa
     4021 tcctgtttta ttcaattctt tttgtatagc gaccactccc tatctggaat tgatacgtaa
     4081 tcgtccgctc tttttcttcc caataaaatt gataagcttt tagtgaactt tcattcccgg
     4141 ttctagcagc aaaagtaata gaagcatggc gtttcaaggt aatctcatca ggaatatcta
     4201 atacttccca atttatttta tctggattcg gaacatgaaa aatgatttga ttctcttcac
     4261 tattccaata aaagcctgtt tggatctgat tcacaatagc gattttttgc gtagaataga
     4321 ttcgtttttc aaattggccg aaaaattgat agactgccaa ttcattcttc catgcagaaa
     4381 atgcgagaac tggaaaagaa aggatcgttg taagtaataa aatcagtaag agtgattcaa
     4441 acaacgtata tcctgatgct aaaaccttac cttcaaggcc gctgatagat ttctgcatgt
     4501 tctcgtttga tatagccttc attcaacaat tcctccacag taggaactct tccattattt
     4561 tgaagcgcat agagctcttt ttgtgtgtct accaactgga tgatcgcttc acgactttct
     4621 tggtctacat gattcctata acgcgacaaa ttagggacga acaacaacac taatacactg
     4681 atgatcagta agacgattag catttcgatc aatgtaaaac cgctataata agatattttt
     4741 ctctttttca tggcaaaacc tcctctatgc ttccgtaaac aggtaataaa acagcggcat
     4801 aaactaataa aatcaaagcc gctacgccca aaaaaatgat tggctgaata aaatgcatcc
     4861 agtgatgaag acgtttgaaa agctgttttc ttgtccattc gctgtaaaaa atcagttctt
     4921 tacctaaact tccctttgcc tctccctgta aaacaatttt actgaattct tctgatagaa
     4981 aacgataatt ctggaactgc tcagccaatg acatacctgc ctccaatcct tgaatcaact
     5041 tagaggcaag atgctgaatc agactttctt gtttcgtttc ctttaagcag actatgattt
     5101 gccttaattc gaaaccttca tgaaaaagct ttccaatctc tagtgacaaa taagccgatt
     5161 gatacaagga atatatcggt ccaatcaata attgtctgct tatccattca ctctgatcaa
     5221 caggagacaa cttcatcaga cgatacctta ctagtaaagc caatagagta accgctagga
     5281 tgaatagtag aacataccaa tgaaagactt gtatcagctg aataccccaa tgctcttgca
     5341 tgaccattcc tgaaccaata agctgaggca aaacgaacaa ccgcatacta aacaagatac
     5401 ctagcaaaaa ggtgagcaat agcatcggat atgcaagcag ctttttcaaa tcattgcgaa
     5461 atccttcaac aagccgaaac tgtttggcaa tttccaacaa agttgtcggc agattcccat
     5521 gacgttcagc taactcgatc tgtaccagtt gctctgctcc atatcctatt tgcgaaagta
     5581 cctcactaaa cgcttgccct tcctttagct gatgttggat atttgccaaa ataacaggtg
     5641 gaaacgaacg gatcgtgagt aataaataaa gggattcttg cagactaaaa ccagataaaa
     5701 gcagttctcc catggtttgt gcaaactggt tttgctggct tcttgttaat cctttagaat
     5761 agttctgact gccaaatatt ttcctgagct tcttcataac tttcctccca tgattttttt
     5821 actgattctt cctccataaa ttgataactc cataaaacag aaggatggtg attggcatct
     5881 tccaaaagtt cttgataaat gacaccagct aaacattcag atagctcttc ttttttcttt
     5941 gtcagttcat ggattctgac caatgtacct tcaagattgc gtgcatgtac ggttgcaaaa
     6001 acccggtgcc ctgttagtgc tgctcttacc gcagcatgtg cagttgattc atctcttatc
     6061 tcgccaatga ttagcaagtc tggacgatgc cgtaatgcga gtctcaaaag tgtatcataa
     6121 gtctgtgcta ttttcggatt gatctgtagc tgcagaaaaa acggttcttc tatctctaca
     6181 ggatcttcga tagtaatcac ttgcaatttt tcttccaaag ccaaccgata cattagagaa
     6241 gtcttgcctg atccgactgg tccgctgaaa aggtataatc cacgtctgac ggttttctgt
     6301 aaaatagttg caaaatctcg agaactaaag caacgatggg gtgtttcaaa agtaccgtat
     6361 aatagccgaa tcactaaact ctctcgttga aggtaatctc ctacagaaga caaccgcaga
     6421 cgaacttcct gtttttctaa agaataagta aatgcaccta actgagcttt tcttttttct
     6481 ccgatatcca tctgtcccaa atacttaaat cttgagatca gttgctgtcc atctgccgaa
     6541 gagactttct tccagaatgt tcgttttttt ccatagcgaa aatagatgta aaatacttct
     6601 gaagctggca atatatatac gtcttgggca tgtttggcaa aactttcact tatcatttga
     6661 tccgataatc gttttatttc cattattctc ccctccacaa aaaacatacg tctttttgat
     6721 ctttttgttt caaataaaat gaatttacaa aaaaatattg gcaaaaaccg aatttatgtt
     6781 atacttataa aaatttatct tgggaggcac tccattggaa acaacattaa ggaaatatat
     6841 cgaagtagca agtaaaaaaa agcctgctga cctagtgatc aagcatgcta agatcgtcaa
     6901 cgtctttacg aaagaaatca tggaaaaaga tgtagcaatc tgcgaaggaa tgatcgtagg
     6961 aatcggtgaa tacgaaggaa aaagcgtaca taatgctaat gggcaatatc tagtgccagg
     7021 attcatcgat ggccatgtgc atatcgaaag ctcactttta tcaccaagcg aattttcaaa
     7081 agtatccttg cttcatggag ttactactgt agtgacagat ccccatgaaa taggaaacat
     7141 tgctgggagt acaggtcttg agtttatgat agaagatgca agacaaacac tcatgaatat
     7201 cttttttatg ctaccttctt gtgtacctgt aacccctttt gagaccaatg gtgcaaattt
     7261 aaatgcaaaa aaattagccc cttttttaca aaaacccgaa gttttaggct tagctgaagt
     7321 catgaactat caagcagtag caaataatga aacagacatc ctagaaaaga tccaattgat
     7381 gcaccagcat aagaaaaaaa ttgacgggca tgcagctggt atcggcatgg aagagttaaa
     7441 tgtttatcct gcagcaggta ttcggacgga tcatgaagct acaactgcaa aagaagctaa
     7501 agaacgtttg gatcttggta tgtatttaat ggttcgagaa gggaccgttg caaaagatct
     7561 ggcatcgcta ctaccggcga tcacacctga aaattcaaga agatgcttct ttgtcaccga
     7621 tgataaactg atcaatgatt tagtaaaaga aggatcaatc gatcatatcg tccgtcaagc
     7681 tattgcactt ggaatcgatc cattacaagc cattcagatg gcaacattga atgcagcaga
     7741 atgttttgat ttgaaaacac taggtgcgat tgcccctggg tatcaagctg atttcttctt
     7801 aacagacgat cttaccaagc tgccaattca tgaagttttc tataagggaa cattagttgt
     7861 tcaatctggg caactccaag accatttatt tgctgatcat tcaaattcat atacgtgttg
     7921 tctgccaaaa ctaaacgctc aaccattaaa tccacattcg ttagagctgc ttctttcttc
     7981 ttcacaagcc catgtgatcg aaattatccc gaatagtcta gtgactaaag atttggtaga
     8041 aaaagtggac cgacaaggcg aacgattcat tccttcaatc gaaaaagacc aattaaagat
     8101 tgcggtgatc gagcgtcacc atcaaacggg aaacattgga ttaggcatag tgaaaggatt
     8161 tcagctgaaa caaggagcga tagcgacaac gattgcccat gactcgcata atctagttgt
     8221 cgttgggaca aacgacgagg atatgctgta tgctgctaat cttttgattc aaaaaggtgg
     8281 cgggatgacc gttgtcaaca atcagcagga aatagcttgt gtttctcttc ctataggcgg
     8341 aatcatgaca caggaacctt tttcgatcgt ctatgaacag ttgaatgaat taataactaa
     8401 agcacatcaa ctaggagcag ctaaaacctt tgatcctttt ttgacacttt ctttcttaac
     8461 attatctgtc atacccgaat tgaagatcac cgacaaagga ttattttcat tttctcgctt
     8521 ccaattgatt ccaccttgtg gaaaaaccga tgaaataaat acattttaaa tcaaataaat
     8581 cccagaaaat acattacatc tgtaatgttc attttctggg atttatttat ggatattaat
     8641 gtttttatga aagcagaaag ttagtttcac agacttttct ttttggtctt atcttgaaat
     8701 tgagcttcta ctcgataaat acgttgacct tttgaagaaa atttttcttc atactccgtc
     8761 ataatattgc cttcaaattg actttgatgt aaatctaacc atacctgttt gaggatcatg
     8821 ccatattgag aaaaacttgc cagagaatac tcaaacaatc cttgattatc tgttttgaaa
     8881 tggatttctc catttggacg aagaatctgt tcatctacag caagaaaagt cttatatgtc
     8941 aatcgccgtt tttcatgacg tttttttggc catggatcgg aaaaattcag atagatttga
     9001 tctacttcgc tgtcagcgaa atactctgtc aacgctgatc catccacatg tagaagtttc
     9061 aggttaggaa gcggttgttc gatcaacttg tctagtgcaa tcgaaacaac actgacctgc
     9121 atttctactc caatataatt gatttcagga tgggcttttg ccattccagt gatgaactgg
     9181 cctttcccca taccaatctc aatgtggatt ggatgatcgt tttcaaacaa ttcattccat
     9241 tttcccttcc acaatgttgg atcactaatt acaaaattcg ggtgagccgc taacatttcc
     9301 gctgctcccg gacgatttcg gacacgcatt atttatctcc attcctttcc tctaattaaa
     9361 aaggtctggc caaatctccg cagagtttgg gccaaacctc agccactgaa aattcgtttt
     9421 aattgtaaaa tttcttcgtt catccgtcga tgatcttcac gtttgcaata tctggtgatt
     9481 tcttggagca aattcatggc cgcataccaa tgaaattttt caagcatttc gtcagtcggg
     9541 cgtatgccat aactgatcag ccactgattc cagctagaca gtggcacata atttcctaaa
     9601 atcgttgcaa tatccaccgc agggtctgca aacatgattg aatcccaatc gactagatat
     9661 aaataatttt tacatactaa ccaattacga tggtttacat ctccgtgtac tactgataag
     9721 ttttccttat ctattgacgg gatctgctca cgaagaaaac gatggacaat ttgcagaaat
     9781 tgatttttct ggagttcttc cggtaattct ttttcatatt tttgcagcat cttctccggc
     9841 gtcatgacac gaccaccgat acgggtcagc atggttttaa gagaatttga atgatgtaaa
     9901 tggtacaaaa catcaatcac gtcgtttctt tgaccaatct cattaggagt cagcaagcga
     9961 ccttccagcc actcttgtgc tgtcaaagta tcgccattcc ccgttcgttt tgtccaaaca
    10021 agttttggtg taattccttc ccttgaaaga gcagccaaca tcggagtcgt gtttcgttta
    10081 ataaagactt tgtcctcatc acgcatgcct atgtatgttt ttcctgtgtc gcctttgatg
    10141 ggacgtaacc gccactcttg gtctaactga aactccatca tgcattctcc cgtcctctta
    10201 ctctttgttt tgcagtactt attattctag tcgcctgaac actacacgtc aagcacatat
    10261 acgttctttt tcatttaatt gccattcaaa aaaccagctt ttctcaaaaa aaatagaacg
    10321 aacagcattc ttactgtcaa cgctctattt ttcattctca tttttaccct acacagcttt
    10381 gttttcagtc tgacatcttc ttcaaacgag ttggtacata tagtttaaca aagagcatag
    10441 taacaatcaa ataagccgct agcggaaccc atcgctgctg cccatctaat acgactgcag
    10501 caatcaaacc aaaaataacc gacgcgataa ttaataccca tgtgagtaga tactgaatcg
    10561 cctgcttctt ctgattctta tggattggat aaagctgaac gagaaccata tagcggaact
    10621 ggttcgctaa aggtaacagt tggaaaccga tcaaatataa aaataagaaa ccgataatca
    10681 gactaaaata aaagtcgact actccggcaa tcaataaccc acctataatt actaatcgga
    10741 aataaagacc actaaaatca gaacctcttg ccaatcttct tgcaaacaag taaagataag
    10801 tgttttttga ttctctttta atatgagtca atataggatc caaatagcgt cttcttttga
    10861 tctttgccgt aatttcagga acatctgtga ataaattgat aaattgatag atctgatgca
    10921 acctatgttc ttctacatgc accattttct cccaatccaa tcctttttct tgttgcttcc
    10981 acaaaaacca gtaatataga cttgcctgta ctaatgcacc tgctattcct gcccatatta
    11041 gccaataaaa tgatagaaac aaaaagatca gagaagaaaa aaaccagcct aatttccaat
    11101 aacgaataac ttgttgatct agtcgaaaca attctgctct cttgatttgc agatgactga
    11161 atttcaaact ccataatatg atcagataga cgaaaaacat cgaaaaggat tgctccgtac
    11221 taaccaccac taatggcatg gccaatccgc atgccaatat caaaaagatc aacggaaaaa
    11281 tgcaagaata gcgaaaagct cgttccaaat aggtttgcat ttctttttct ttaggaagta
    11341 aaaaaacaat atctgctgcc tctgcaaaag tagccatctt acccacgaat aaacagagca
    11401 tccaaaatac agagattatg agccctccaa ttgcaaacgg tggctgcagg gttttcaacc
    11461 aattagaata atataatccc acaccgccaa tcaagaaaag acaaactaaa acaaaatgat
    11521 cgttcaatac ataacgcata tatttcatca ttttcttttg atgtcttgct aaacgttttt
    11581 gaaaaaaact actcatgtgc ctgctccttt gttaaagcaa tatagatttc gtccaaagaa
    11641 gaatccggca gctgaaattc ttttctcaac atctcaagag aaccgtctgc tcgtatctct
    11701 ccgttatgta agaccacaaa acgatcacaa tatttttctg cagtagctaa aatatgcgta
    11761 gacatcaaaa taccggctcc ttcttttttc atttctacca tcaattcaag caatgcattg
    11821 atagctagtg gatccaagcc taaaaatggt tcgtcgatga tatataaaga aggctcgatc
    11881 aaaaaggcgc ataagatcat cactttctgt ttcattccct ttgaaaaatt agctgggaac
    11941 cagtcaagac gattagataa ccgaaaagtc tccaacaact tttctgctct tttcattgct
    12001 atatccatag gtatatcgta ggccatcgca gtgacttcga tatgttctct caatgtcaat
    12061 tcttcatata aagaaggagt ctctggaata tacccaattt gtttgcgata ttcttccgta
    12121 tttttcttta aggatagatg attgatttct atcgttcctt ttgaagcttc cagcagaccg
    12181 ataatgtgct tgatggtcgt acttttccct gctccgttca gtccaatcaa ccctactatc
    12241 tctccaggct gtacatcgaa actgatatct tttaacacag ggagatgtcc gtacccccct
    12301 gtgacatgat cgataattaa actcattctt tttcctccgc catttatttt tatttattat
    12361 accacgaaga aaatgacggt attttgtttt tttccttaca atagctttaa cttatggtaa
    12421 agttaagctg aagtatttat tctttataga aaggatgttt tatatggaaa actgtatttt
    12481 ttgcaaaatc atcaatcaag aaatcccaag ctataaaata tatgaagacg acaaggtcta
    12541 tgctttttta gacatctctc aagcaacgaa aggacacaca ttagttgtgc ctaaacgtca
    12601 tgtagcagat atttttgaat atgatcccga acttgcagga gaggtttttt cgcgtgtgcc
    12661 taagattgct caagcattgg aaaaagcttt tccagaaatg aaaggtctta atatcttgaa
    12721 taacaaccgt gaactagcct atcaatctgt ttttcattca catatccatt tagtccctcg
    12781 ctacagcaaa gaggatgatt tttctattca ctttgtcaat catcaagata gttatggatc
    12841 ggaagaacta aaagctattc aagaaacaat cgtaaagcag gtgtcctgcg atgattaaaa
    12901 actttttcaa aggactgtta tttggagctg ctgctggcac tgtcggaggt ctgttattgg
    12961 cgcccaggag cgggaatgaa acaagaaaga aactgatcga agaattggaa gaagcaacaa
    13021 ctctgacaaa tgatttaaat aacagcctga atcattttaa aagcgcattg atcgaaacaa
    13081 aagaaacagc tgaatctgtg attcctgctt tccaagaagc aatcaaaaaa gatatcgaag
    13141 attttaaatt ccaagccgaa ccacgtatca cacaaatcaa tgaacaagta gaaaaactaa
    13201 aagctgatct gccagagtat cctgaacaaa tataataatg attattttct caacagattt
    13261 aactatataa gtgagaaaag tttgcgt
//
LOCUS       DASJSZ010000072        13086 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.074, whole genome shotgun sequence.
ACCESSION   DASJSZ010000072 DASJSZ010000000
VERSION     DASJSZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.074"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(79..2199)
                     /locus_tag="U4B03_002113"
     CDS             complement(79..2199)
                     /locus_tag="U4B03_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2068954.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="U4B03_002114"
     CDS             2429..3607
                     /locus_tag="U4B03_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEN2068955.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="U4B03_002115"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="U4B03_002115"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEN2068956.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="U4B03_002116"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="U4B03_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEN2068957.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="U4B03_002117"
     CDS             complement(4960..6213)
                     /locus_tag="U4B03_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2068958.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="U4B03_002118"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="U4B03_002118"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEN2068959.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="U4B03_002119"
     CDS             7790..9136
                     /locus_tag="U4B03_002119"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN2068960.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="U4B03_002120"
     CDS             complement(9444..9830)
                     /locus_tag="U4B03_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2068961.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="U4B03_002121"
     CDS             complement(10098..10247)
                     /locus_tag="U4B03_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068962.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="U4B03_002122"
     CDS             10709..10921
                     /locus_tag="U4B03_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2068963.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="U4B03_002123"
     CDS             complement(11199..12998)
                     /locus_tag="U4B03_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN2068964.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaac
//
LOCUS       DASJSZ010000073        13019 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.075, whole genome shotgun sequence.
ACCESSION   DASJSZ010000073 DASJSZ010000000
VERSION     DASJSZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.075"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..563
                     /locus_tag="U4B03_002124"
     CDS             <1..563
                     /locus_tag="U4B03_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2068965.1"
                     /translation="FDQSGPERIKQAIAREASKYINDYDTLFINSSSTALKTLDFLQS
                     NHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTISSVNADICIIGC
                     SGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKFKIADISVFDYLI
                     TDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(836..1663)
                     /locus_tag="U4B03_002125"
     CDS             complement(836..1663)
                     /locus_tag="U4B03_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2068966.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            2231..3466
                     /locus_tag="U4B03_002126"
     CDS             2231..3466
                     /locus_tag="U4B03_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN2068967.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(4465..4725)
                     /locus_tag="U4B03_002127"
     CDS             complement(4465..4725)
                     /locus_tag="U4B03_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN2068968.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(4752..6053)
                     /locus_tag="U4B03_002128"
     CDS             complement(4752..6053)
                     /locus_tag="U4B03_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2068969.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(6345..6803)
                     /locus_tag="U4B03_002129"
     CDS             complement(6345..6803)
                     /locus_tag="U4B03_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2068970.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(6816..8147)
                     /locus_tag="U4B03_002130"
     CDS             complement(6816..8147)
                     /locus_tag="U4B03_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEN2068971.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(8158..9600)
                     /locus_tag="U4B03_002131"
     CDS             complement(8158..9600)
                     /locus_tag="U4B03_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2068972.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(9587..10351)
                     /locus_tag="U4B03_002132"
     CDS             complement(9587..10351)
                     /locus_tag="U4B03_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEN2068973.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(10455..11330)
                     /locus_tag="U4B03_002133"
     CDS             complement(10455..11330)
                     /locus_tag="U4B03_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2068974.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(11585..11669)
                     /locus_tag="U4B03_002134"
     tRNA            complement(11585..11669)
                     /locus_tag="U4B03_002134"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11633..11635),aa:Leu,seq:caa)
     gene            complement(11687..11758)
                     /locus_tag="U4B03_002135"
     tRNA            complement(11687..11758)
                     /locus_tag="U4B03_002135"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11724..11726),aa:Gln,seq:ttg)
     gene            complement(11768..11840)
                     /locus_tag="U4B03_002136"
     tRNA            complement(11768..11840)
                     /locus_tag="U4B03_002136"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11805..11807),aa:His,seq:gtg)
     gene            complement(11902..11986)
                     /locus_tag="U4B03_002137"
     tRNA            complement(11902..11986)
                     /locus_tag="U4B03_002137"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11950..11952),aa:Leu,seq:caa)
     gene            complement(12007..12077)
                     /locus_tag="U4B03_002138"
     tRNA            complement(12007..12077)
                     /locus_tag="U4B03_002138"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12043..12045),aa:Cys,seq:gca)
     gene            complement(12123..12194)
                     /locus_tag="U4B03_002139"
     tRNA            complement(12123..12194)
                     /locus_tag="U4B03_002139"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12160..12162),aa:Gln,seq:ttg)
     gene            complement(12206..12278)
                     /locus_tag="U4B03_002140"
     tRNA            complement(12206..12278)
                     /locus_tag="U4B03_002140"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12243..12245),aa:His,seq:gtg)
     gene            complement(12281..12354)
                     /locus_tag="U4B03_002141"
     tRNA            complement(12281..12354)
                     /locus_tag="U4B03_002141"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12320..12322),aa:Trp,seq:cca)
     gene            complement(12361..12441)
                     /locus_tag="U4B03_002142"
     tRNA            complement(12361..12441)
                     /locus_tag="U4B03_002142"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12405..12407),aa:Tyr,seq:gta)
     gene            complement(12443..12518)
                     /locus_tag="U4B03_002143"
     tRNA            complement(12443..12518)
                     /locus_tag="U4B03_002143"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12483..12485),aa:Phe,seq:gaa)
     gene            complement(12554..12626)
                     /locus_tag="U4B03_002144"
     tRNA            complement(12554..12626)
                     /locus_tag="U4B03_002144"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12591..12593),aa:Asp,seq:gtc)
     gene            complement(12636..12708)
                     /locus_tag="U4B03_002145"
     tRNA            complement(12636..12708)
                     /locus_tag="U4B03_002145"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12673..12675),aa:Val,seq:tac)
     gene            complement(12724..12795)
                     /locus_tag="U4B03_002146"
     tRNA            complement(12724..12795)
                     /locus_tag="U4B03_002146"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12760..12762),aa:Glu,seq:ttc)
     gene            complement(12810..12899)
                     /locus_tag="U4B03_002147"
     tRNA            complement(12810..12899)
                     /locus_tag="U4B03_002147"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12863..12865),aa:Ser,seq:gga)
     gene            complement(12939..13012)
                     /locus_tag="U4B03_002148"
     tRNA            complement(12939..13012)
                     /locus_tag="U4B03_002148"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12976..12978),aa:Asn,seq:gtt)
BASE COUNT         4184 a         2720 c         2069 g         4046 t
ORIGIN      
        1 attttgatca atctggtcct gaaaggatta aacaagcgat tgccagagaa gctagtaaat
       61 acattaacga ttatgatacg ctgtttatca attctagctc gactgcgttg aaaacactgg
      121 attttcttca atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc aattactctc
      181 cgcacaaaga taattgtagt tatattttaa ctggtggaga gatgcgcttt ccaaaagaag
      241 cactagtggg cgacatcgca ttaaatacga tttcttcagt caatgcagac atttgtatca
      301 ttggctgtag cggagtcgat gtagtacatg gggtcacaac aaagatatta aatgaatcaa
      361 cgatcaacga aatgatggtc aacaagacaa tcaaatgtaa gattttagtc gctgatcatc
      421 gaaaaatcgg actgacatca aagttcaaga ttgctgatat ttcagtcttt gattatttga
      481 tcactgataa attttgttct gaaaaagtat tagaggagat cagaaatacg gggataaaaa
      541 ccattcaagt agattcattt taagaaccag gacgtaaata tgtaaatagg attatcaaaa
      601 aaatgaataa caaatttatc gataaatgaa aaacacgcta cgctcaaatt gataaaggaa
      661 tcccaagatg tatccatcca atcgatacca tcataatcta ttgcttcttt tataaccgtt
      721 atatgttgcc taaataggaa tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca
      781 cctccttttt tcgaataaac ggtattcaaa tcagaacact gatttcacaa ccactttatt
      841 tcaggtcgtt ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc gctatttttt
      901 ttgcgtccat atatctttct aaataatcat cttctcctaa ttgcttatgg caagaagcaa
      961 tttcataaag caagtattcc aatgcataag tcgatttatg tttcttatta tactcaatac
     1021 ccttctcaca taattggatt gctttatcat aatcctcgat tttacggtaa taggtggcga
     1081 tatcataata tacaatgcag accatcagat tatcaaaatc agtctcactc aatattttaa
     1141 gtgtacgctc cacataaact ttcgcaaaat ctaattctcc ttttgacaag tagatcattg
     1201 ccacgtttgc agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt tccaacactt
     1261 gattaaaata aaataatgct gcatctggtt ttttcgcata aagatgatca agcagtcctt
     1321 gataaaataa aatttgattt aaaagatcag aattacttac tttttcctga tcgatcgttt
     1381 gtaaaatttt ataagattct tggtattttg atacagaatt caatgattga atctcattta
     1441 tttttttcct gattttgtct tcttctgttt ctactaatat atcggagatc tcgacattca
     1501 gctttttgca aagcgcactt aagatattga tgcctggcga catattcttg ttttcgatat
     1561 tgctgacagt tgcttgttca caaatgcctt ctgctaattg tacttgtgtt aacccctttt
     1621 tctttcggta ttcttttagt aatgctccat ctactttaat caaaacaatt tccttccctt
     1681 taaaaaatta tttctccttt atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt
     1741 ttagaacaga ataaaaattt taattttaaa ataatattca aattaaacaa ctggcgtatc
     1801 tgctccttga tttctttttg tgttatctga ataattatcg tttacagtta taacacatag
     1861 tgaaaattgt catggcgaga aagtctaaga atatttttaa acataactat ttctttctta
     1921 cgtgtgtata atgacgacag cacacctatc aattaatata attattttat ttttttatgt
     1981 tctcatgttt atttttaatg acacaacttc tcgtatttat ctggcacaac atataaatcg
     2041 atcaaaaaat tgttgcgtgt ttttttgaaa attggagttt caacttgatt acaaaattta
     2101 aatttagaag ttgtgaaaac agcgagagac agtttaatat tcaaaatata cataggctac
     2161 tcgaacgctg tgttttttag tatactagta agaaacgaag ggatagactc attccaataa
     2221 ggaggatttc atgaagacaa ttctttttgc tcctgcagta ttcaatttag ctgagacaac
     2281 acgaatgatt gaggttgcca aagctatgaa agaccgttat tactgtgtat tctttggctt
     2341 ttctaaaact tttgctcctt tgatcacaga aagtggcttt gagtatcatt tactagcgcc
     2401 gatccttacg aaaaaacaag aagaacaaat cataaaattt gaccagttgc gtacacttaa
     2461 aaaccctttt accaaagaaa tggttgcctc tcgtatcact agtgaacaga gtctgattgc
     2521 taagtttgag cctcgactga ttgtgacagg ttcgaatgtg acaatttttt tatcggcccg
     2581 caataaaaag attcctttag tatatgttaa accctatgct ttgagtgaac ctttttttca
     2641 atcagagcat gcgctgatgc cccacgagtt acgtttcttc ggccctttaa gcaatcccct
     2701 ttggcactgg acaaaaaagc gtatagtaaa tacccgatgg atccccaatg ccttcaaaca
     2761 cgctgcacaa aaagaaatgc ttcctttacc aaaaacaagt ttacaaatga tggatgctga
     2821 tctaaatctc atcacaacgc ctaaactatt tttggaagaa gaacagctgc cggaaaatta
     2881 tcgaatcgtt ggtccaattt ttgctaagct cagtacttcc ttgcctgaag aagtgctaac
     2941 ttttatacag cgaaagcaac aagaaagaaa acgtattatc tatttcgcaa tgggtagttc
     3001 aggcaatccg cgtatgttac gcaaaatttt gttcttctta caaaaacaac cggatctagc
     3061 ggttattgca cctattgact atctgcttga tgacattcaa tctgactcag aagatctttt
     3121 aataacagaa tatctgtcta gtcatctttt gcatgaattc attgattttt cttttatcca
     3181 cggcggggaa gggaccgtcc aaactgcctg tgaatccggc aaaccgttta tcggtgtggg
     3241 gatgcaattt gagcaaagct gtaatattga ctattgtgta aaatacggaa acgcaatcgc
     3301 cctcacaaaa ccagtgactg aaagaaaact gcagattgct ctgcgcgaag tttcttcacc
     3361 caagattcgt cagcaggcac gcacgctgca agccgttttt ccaataaatg gtccacaaca
     3421 agcaattcaa gaaatcgaaa ccttcctgag caaccaccca atatagtttc aagtagaaaa
     3481 agctttttta atagagactc atcagtaatt tcgctattcg agacccagac agtgaaaata
     3541 caaaaaaagt aaatagtgga ttgaaaagtt agagttactt aaattacggt gtctaaaaaa
     3601 cacaaaatca tccttaaaat aaaagatctg acgctatcca aatgagcata ggtagcgtct
     3661 gccatttatt cttagtcaaa ttctttgaca cctttctctg gttggatcat ctcaacgttt
     3721 cttcaaaaga aagaccataa taattaggca aatacatttt cttaaaatgt gtcatataga
     3781 tctagaagca tcgctttgta gagatttgtg ccgctagtcc cctcaacaat tactaaatca
     3841 aatgtaggat ttaccacttt atctttgata atcaacctcc ctcttctgtt gagacaatga
     3901 tctcgcaatc cgatgcatac tcctatataa actgccagca atccccacat attgagtaga
     3961 tcatgtcaga atgttctatg tcgtcagcaa cgatacacta aaagtcacgt tcaccatgac
     4021 tgattgctat tcccagcaca attatttcta tacacgtttc atgatttgaa taaacactca
     4081 catcatacat attaccattc atacaccata ctgcagcccc tactatatgg ttgtaagctc
     4141 ccaaatctat atttgacttg atcactttgg tagtagatct actaattcta aatccattgc
     4201 tgataaaatt tccatttttt cctccttctt tctgccaaaa cagttaaaac aataataatc
     4261 tttatgtaaa aaagggctgt gacaaaagcc ttgtcacagt cccatattcc gaataaacgg
     4321 tggtcaaaag cttgcatctg cggtaataag ttcaaccaaa cgaaaaataa gccaacattt
     4381 cacaaaaatt taagaagcaa ttttcgtaaa tttcttctta tttcttgaag ctaaccactt
     4441 ctgttacaac cacttttaga acatttaaag cttcgatgtg acaaaccatc tatgctgctc
     4501 gatcataaaa ttgatagcaa aaattccaca aagtacatat gtcccccatg aaaacaaaac
     4561 attcatatgc ggtacaatga tctttggaag aatcacgatc aaaaatgatg ccagtgccca
     4621 aatccatcga ttgatttgaa tatgatatgt agctagcata ttcaatataa ggtgactagc
     4681 cgttaataaa aagataaaaa caaaaaataa aatcagccaa tacattgttt cccaccttct
     4741 aaaaaccaaa cttagacagt ctggacttcg ttttctccac gactttgttc atctgacagc
     4801 atctttttat cataagcttt tacaaatgga taatagataa taaatgctat caatgtgttg
     4861 atcatgatca agataacaac acgccagtca tttcctgctg aaagataacc gccgattgga
     4921 gctggtaatg tccatgttgg caaaacagta aatctattga ccatttctaa tttgactgca
     4981 aaataactga taatagtcaa aactaaagga actaagttga acgggatgaa catgtaagga
     5041 ttcaacatca taggagctcc aaaaattatg ggctcattga tattgaagat accaggaata
     5101 atagaaaaac gaccaacctc tttcaagaat tttgatttac agaagaatat aaacaaaaca
     5161 gataccacga tcgttgctcc tgcaccacca attgttaccg tccattgata aaactgttca
     5221 ggagcgatat agggtaattg ggtggcagct gtaccatctg ccaatgcctt tgcattctca
     5281 tccaacatga tcagccacat tggacgaaca acagccccta caatactcat gccatggata
     5341 ccaaatgacc acaacaaatg gatcaaaaac attggtagta aaacaccaag caaattattc
     5401 cctaaaatat tggttaaagg ctcgaataac gataataagg cagcgtttac atcaaagccc
     5461 gcgacgcttc gaatcagcca agtaattgtt aaaatgactg cacctggaat caatgcctca
     5521 aaactacggg caacagcagg tggcacttgt tctggcattt tgatagtaat atttcgattt
     5581 ttaaagaaac gatatacttc cactgcaaaa atcatagata agatccccgc aaacatccca
     5641 gatgctccca aagaactcat aggtaagacc cagccaagag gtgcctcttc aggagttaaa
     5701 acgtttacag gaacttgtaa cataaagaac gttcctaaag ataatatacc acctgtcaca
     5761 ctatctaatc catatgattt agctagatcc gaacccataa caaaagttgc ataaatagcc
     5821 atcaatccca tcgtcatcct aaagggtaaa acaatcgttg ccgaataagg ggcaataaat
     5881 tcattccatc caggaaacgg taaattgata aagaccataa aaaatgtccc aatcaaaatc
     5941 aatgacaatg ttgaaacaac cccattacga attgctaata aatgacgctg tgtaccaatt
     6001 tttgccattg gtgggacaag tttctcttcg acccatttca tcagcttttc cactaaaacc
     6061 actccttttc aataaaatat gaaccattca ttgcatgaca acgcaaagaa tgttcataac
     6121 taattatata attaagaaat tttatttcaa tatattctct taaataatca aatttatttt
     6181 taagaatcct gaaaataagt tttctaaatc ataggagcaa caaaaaaatt cgataattaa
     6241 caaaaatcaa aatgattaaa tctagtgatt gaaacatatc atttcaaaaa aattttgtaa
     6301 attcacccat aaatatcacc ctatttaact tttttcatcg tacactaaaa ttcaaaacaa
     6361 ctatctttaa tctttacttc ttggttttgt tttaataaat ttccttcaaa aatgcctgga
     6421 gaaactacaa tgatcaccgt atctttttgt gcttgctcca acttcttcaa atctataata
     6481 gctaacagtg tcccttcttt tacctcctgg ttactttcaa cccgtatctg aaacggactt
     6541 ccttcgagtt caactgtatc taaacccata tggatcagta tttttctgcc agattcactt
     6601 tcgatcgtta tggcatgtcg ggtaggaaat atattatcga ttattccttt aactggcgca
     6661 tggatatatc catcatgatt cgttatagcg tatccaactc ctatcatttt tgaagaaaag
     6721 acctcgtctt ttactgctgt taatggaata atttgaccac tagctacagc ttttagaatg
     6781 tttcttgttt taaagaaatg cactttttat cacctctact ttaatgctgg ccaatatttt
     6841 ttgtttactt tgatcatttc atctagtatt ttttttgcga ctctggcatt tggtacggtt
     6901 tgattcaatg taaatgcttg taaagctttt tgataagaac cttcaaagta agcatctact
     6961 agtaacttct ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc actgattggt
     7021 tttcttagtg taattggctc cacaccagtt gcaccaacat atgccggtat ttcaacaact
     7081 gcatctgacc gtagattcgg aatcgcccct tgattaggaa cgattagcat aaagcgacga
     7141 cgatcatcat tcaacaagga aatagctata tcgacgatat attgtccatg ctccccaaaa
     7201 ttaaagttca atacttcttc cacaataccg cggcgtattt tatcagccat ttctttggta
     7261 tttttctctc gtccatccat gaccatatta gcacgtgtat actctttgtc agtatgctca
     7321 acgaccatat caggatataa gtaatactct aagtaattat tcgggatatt acttggaaaa
     7381 tttttcgtga tgactgacat catttgaaag gttttttgcc aagttttatc tcctatatta
     7441 aaatcggcga cttgcatttc ttttgttatt agtttttcaa taatctctgg cataatttct
     7501 cgttgcaatt ctttatcata aatcgatgta taccagccaa agtggtttaa accataatac
     7561 gtaacgatcc aattcttacg atcataaccg taattcactg caatcgtttc ttcaattgaa
     7621 atagtcatat cacaggcatt aatcattttt acattcggat atttacgacg aacagcttca
     7681 gaaacgatcg actctggatt tgtataattc aaaatccatg ctttgggggc atagtcttgt
     7741 atatgatcga caatttccaa taaacctttg attgagcgca tcccataagc aaatcctccg
     7801 agtccacacg tttcttgtcc tactaatcca tgtttaagtg gaatcttttc gtccttttct
     7861 cgcatctcca atcctccaac acgaatttgt gagaaaacaa aatcgcatcc tgtaaaagca
     7921 agctttggat catccgttgc acgaatgcga atatttgaaa aaccttttct tttcagcata
     7981 aatttaacaa tcaagtacat atccatattt ctttcttcat caatatcata taaacgtatt
     8041 tcagataatg gcaaccgttt ttcattgttc aaaatggctt gaacgattcc tggtgtatac
     8101 gtactcccgc ctccagctat agtgataatc tgttttttca tcatcatctt ttcctttcta
     8161 ttcttctaat aatttattga attctgccat aacataagtc acttctaagc cgatgataat
     8221 ttgataattt ttttcagttg gtttaatgat cccgcttatc cctaattgct tgattcgctg
     8281 ttcctcgacc atggtgggat ccactacctc tacccgtagg cgtgtattac aactatagac
     8341 atccaggata ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca tatattcata
     8401 atttttactt gctaacccta actctgtttc ttctaaggtt acttcattgc caaactctgc
     8461 cttagcttca cgtcctaatg tctgaatgtt tctcttattg atcaaataat aaaaagtaac
     8521 gtaatacaag gcaaaaaaga aaattccgat tggaagaatc aaaatagcat tggtagcgat
     8581 tcgaaaattg atgagataat caacaataca tgctccgaat gtaaaaccaa ttcgtatatt
     8641 gaataagtaa cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca cgtacaacat
     8701 aggagaaaga aacatgaaac taaattcaac aggctcagtg atatttgcaa taaatgaagt
     8761 tcctgcaccg cttcccatga ttcctttgac catctcttta ttttctttat aagctgcttt
     8821 atagatagct agacatatag ctggtatacc aaacatcatt actacaaaaa aaactgataa
     8881 aaacagtcca gcctgaggat ctccgttgat aaagcgtgtc atctctcctc tgaaaacttc
     8941 cttgctagga gatgtatagc ttcctaaatc gtaataaata tacgtattca acacatggtg
     9001 cagtccaaag gggatcagca aacgattcaa aaacataaat atccccacac ctaatgctcc
     9061 catatcaacg agcagttttg caaaattatc aatcccaatt tggatgagcg gccagataaa
     9121 gcccaaaaaa acagctgcga ttgtttgtag aatcatcacc atagtcaatg ggaatttttc
     9181 gcccgcaaaa taagaaaaaa ctactggtaa tttttgttct ttaaagcgat tatacgtcca
     9241 tgccgctact aaaccggaaa taattccacc aaaaatcccc atattcactt ctgggtccat
     9301 aatttctaag ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg caggtattcc
     9361 tttgtcctta tttttgacaa aaccgattgc aactcctata gcaaataaga tatccaagtt
     9421 tccaaaaacg gcatttccag ctgcaccaat tactgggatg ttcagcatat cgtcagctga
     9481 taaacgcatg atcaatcccg ctaatggcat cgctgcaact ggaagtagca tcgctcttcc
     9541 aagcttctgc acatttgatt taacattcat ttttattcct cctctattaa atatcctcat
     9601 atgttactaa ctcaatcaaa ggatcatttt tcactttttg agcaaatgct gaatgaatta
     9661 aaaattcgac ttggtgggta cgaggataca caaaacttga accatttaac aattcctgat
     9721 ccaaatacgc tgcgtgacac atgacttcga tcgttttatc ttctctcaat atcatgagta
     9781 aacttgccaa atattcttcc acactcgtat tgatttgtcg ttttttttca tcagtttctc
     9841 ctacgacatc gaaacaaggc tcaaaataat cgacatgacg aacttcatct tttcgttcaa
     9901 agttgctccg aaccggcaaa tcatattttt ttgctaatgt aacaactacc tcctgatttt
     9961 catccaaagt atgagtatga tgatgactgt ctaaatgagt tggtatgatc cccgcatgat
    10021 aaattttttg aatctgagca ttccattcct gatacagttg atctttgtca atgttaaatg
    10081 gatgctgata ataagataag gatctgaact ctccgctttc gataagagta tcgacagttt
    10141 tgaggagtgg tttgccacac gtcaatgtta aatggactcc tacacctagc ctaggcattt
    10201 cctttcttag tttcacagca tgctcgaaac ccggcatatt tgccatcaat gtagttgatg
    10261 tcaaaattcc tcgctgataa gcatccataa tcccataatt cacaccatgg ctatagccaa
    10321 aatcatccga attaaaaatc acttttccca tttcattctc cttctttttt ctactaaaat
    10381 aggcgcaacc aaataatcat agtccattta ctatgtgtca tttggttgcg cctatagata
    10441 gttacgccct tattttaata gattcttgct agatgaattg ccaaatagat gatttcattt
    10501 tcttgaatgg caagttgaaa ttgctgttcg atgatatctt taatctttag tgctgtacaa
    10561 tacgctttgc tatgtttaaa tgtttcagta aaaaaaccaa tattttctac tggcgtttct
    10621 tcctgataaa tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg atgataagtc
    10681 agtgattctt tatcataaac gtttgaatag gatttttcta gatgggacaa aatcgtggtc
    10741 gtcatttcta ctatttttaa gctctttaaa ctattaggct ctcctagctc agcattaatg
    10801 atatgcatag tgatgaatcc tacttcattt gaccctaact cggtttgata tcgatgattg
    10861 attcgctcga ccacttctac tgctatatca tattcatttg gataaaggaa tctcatttct
    10921 ggcaacataa ttgctgtgac atctctatct tctctttttc gttgaatagc aaaataaata
    10981 tgatctgtca atgtaatata gatatttgaa tctaatggag aggagatcct agctgttatc
    11041 agttcaatca cttcttccgc aaaatccata aattcgattg gaatctgcga gactatctca
    11101 ctgaatcgtt tgatcttttc tttagaatct aatacaaata atttgtcagc atcctcaaca
    11161 tcaaatggct tactcacttt agcagaaaag ccaatccctt tccccatcgc aattttttct
    11221 attccatcgt catcgatcaa tacagcatta tgattcagct ttttcatcac atacaccgtt
    11281 aattcacctc ctctatatga taaaacaaaa caacttgcag actccttcat acatattttt
    11341 gtctccaaac aatttttaaa gacaaaaaac gacctaagaa agaacccttt cttaggtcgt
    11401 gcctaattga atagtagcac cctacataat ttacaaaaat agtttaacac ataataaaag
    11461 cgttatcaat atctttttta ctcgttttat gataatactt gtatcgaatt gatttggagg
    11521 taattacata aaaacaaaaa aaatatagca actctctata agaaagttgc tatattgata
    11581 attataccgg cggccggggt cgaaccggca cgtcctcaac ggacactgga ttttgagtcc
    11641 agcgcgtctg ccaattccgc cacgccggca aatttaaaaa taataactgg ggtagctgga
    11701 ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaag
    11761 aataaagggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat ccggtgcgtt
    11821 aaccacttcg ccacattcgc catcataaaa gatgttattt aatttctcaa taaaaaacta
    11881 agatgaagac atcttagtca ataccggcgg ccggggtcga accggcacgc cctcaacggg
    11941 cactggattt tgagtccagc gcgtctgcca attccgccac gccggcaagt tgatttatta
    12001 gtactaaggc ggtaaccgga tttgaaccgg tgatgaaggt tttgcagacc tctgccttac
    12061 cacttggcta taccgccatt atactattat caatctataa aataaagaat aaattataga
    12121 aactggggta gctggattcg aaccaacgca tgagggagtc aaagtccctt gccttaccgc
    12181 ttggctatac cccaagaata aaaagggcga atgatgggaa tcgaacccac gaatgccgga
    12241 gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tggcaggggc agcaggaatt
    12301 gaacccacac taacggtttt ggagaccgct gttctacctt taaactatgc ccctataaaa
    12361 tggaggagag tggattcgaa ccactgaacc ctaaggaacg gatttacagt ccgtcgcgtt
    12421 tagccacttc gctactcctc catggtggcg cgggacagaa tcgaactgcc gacacacgga
    12481 gcttcaatcc gttgctctac caactgagct accgcgccat attttaaaat tattaattga
    12541 ataacaagaa cgacggttcc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg
    12601 catgttaacc cctacaccac ggaaccagtt cattatggag gttaacggga tcgaaccgct
    12661 gaccctctgc ttgtaaggca gatgctctcc cagctgagct aaacctccaa cattcaatta
    12721 aaatgacccg tacgggattc gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg
    12781 cttgaccaac gggccaggta aatttgctac ggagagtaag ggattcgaac ccttgagaca
    12841 gtttggcacc atctacatga tttccaatca tgctccttcg gcctctcgga caactctcca
    12901 gagtgaaaga agctcaatac tctttctcaa aatgagaact ccggcagtag gactcgaacc
    12961 tacgacatca tgattaacag tcatgcgcta ctaccaactg agctatgccg gaataacat
//
LOCUS       DASJSZ010000074        12905 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.076, whole genome shotgun sequence.
ACCESSION   DASJSZ010000074 DASJSZ010000000
VERSION     DASJSZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.076"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..3654
                     /locus_tag="U4B03_002149"
     CDS             127..3654
                     /locus_tag="U4B03_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEN2068975.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="U4B03_002150"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="U4B03_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEN2068976.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="U4B03_002151"
     CDS             7442..7783
                     /locus_tag="U4B03_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2068977.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="U4B03_002152"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="U4B03_002152"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEN2068978.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="U4B03_002153"
     CDS             9140..11560
                     /locus_tag="U4B03_002153"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEN2068979.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="U4B03_002154"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="U4B03_002154"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEN2068980.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4385 a         2243 c         2738 g         3539 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaact
//
LOCUS       DASJSZ010000075        12733 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.079, whole genome shotgun sequence.
ACCESSION   DASJSZ010000075 DASJSZ010000000
VERSION     DASJSZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.079"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U4B03_002155"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U4B03_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN2068981.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="U4B03_002156"
     CDS             complement(323..892)
                     /locus_tag="U4B03_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEN2068982.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="U4B03_002157"
     CDS             1078..1980
                     /locus_tag="U4B03_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2068983.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U4B03_002158"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U4B03_002158"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEN2068984.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U4B03_002159"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U4B03_002159"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEN2068985.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="U4B03_002160"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="U4B03_002160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEN2068986.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="U4B03_002161"
     CDS             8548..8733
                     /locus_tag="U4B03_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEN2068987.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="U4B03_002162"
     CDS             complement(8795..9991)
                     /locus_tag="U4B03_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2068988.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="U4B03_002163"
     CDS             complement(10161..11597)
                     /locus_tag="U4B03_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN2068989.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="U4B03_002164"
     CDS             11890..12699
                     /locus_tag="U4B03_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2068990.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2250 g         4010 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tga
//
LOCUS       DASJSZ010000076        12730 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.080, whole genome shotgun sequence.
ACCESSION   DASJSZ010000076 DASJSZ010000000
VERSION     DASJSZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.080"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1760)
                     /gene="uidA"
                     /locus_tag="U4B03_002165"
     CDS             complement(<1..1760)
                     /gene="uidA"
                     /locus_tag="U4B03_002165"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEN2068991.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAY"
     gene            complement(1783..3549)
                     /locus_tag="U4B03_002166"
     CDS             complement(1783..3549)
                     /locus_tag="U4B03_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEN2068992.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(3562..4356)
                     /locus_tag="U4B03_002167"
     CDS             complement(3562..4356)
                     /locus_tag="U4B03_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2068993.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(4366..5742)
                     /locus_tag="U4B03_002168"
     CDS             complement(4366..5742)
                     /locus_tag="U4B03_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2068994.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(5862..6509)
                     /locus_tag="U4B03_002169"
     CDS             complement(5862..6509)
                     /locus_tag="U4B03_002169"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEN2068995.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(6593..8020)
                     /gene="uxaC"
                     /locus_tag="U4B03_002170"
     CDS             complement(6593..8020)
                     /gene="uxaC"
                     /locus_tag="U4B03_002170"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN2068996.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(8094..9173)
                     /gene="uxuA"
                     /locus_tag="U4B03_002171"
     CDS             complement(8094..9173)
                     /gene="uxuA"
                     /locus_tag="U4B03_002171"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2068997.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(9176..10807)
                     /locus_tag="U4B03_002172"
     CDS             complement(9176..10807)
                     /locus_tag="U4B03_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN2068998.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            11111..11782
                     /locus_tag="U4B03_002173"
     CDS             11111..11782
                     /locus_tag="U4B03_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2068999.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(12507..12730)
                     /locus_tag="U4B03_002174"
                     /pseudo
     CDS             complement(12507..12730)
                     /locus_tag="U4B03_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4108 a         2511 c         1639 g         4472 t
ORIGIN      
        1 tagtaagcaa tcattttagg ttttctatct ctagtaaaga ttcctttctt atttccttgc
       61 actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat tccacacttg ttcaccaaca
      121 aaattttcac atgtatctat tacttcgtga tttgcttcgt aataagccac ttgatactct
      181 tctgtaaaca ttgttggtac tgtatcatga aatccagcta ctgtatctgc tccatattct
      241 gtaaacatta ttggcgtatc aggacatctt tccatccatc catctagctc ttttttcaac
      301 agatattttg catgtgccag atcccctccc gaaacatacc aaccataata acgatttaaa
      361 cataaaacat cgacaatatc tcctacctta caaacatctg gcgttgattt tagataagta
      421 acgattgtta caggtctttt ctgcggatca agttctttag ttaattcaac taaaggttca
      481 aaatattctt tagcaccttc ggaatccgaa tctggttcat ttgcaataga ccacattaca
      541 acacttgggt ggttcttgtc gcgttctatc aattccttga tcaccgtttt atgagaattt
      601 tctgttcgta tctctttcca cgtattatgt ttttccacat tatcatctaa taaagtggcc
      661 atgaaattta aatgtaaccc tactgcaggt acttcatcaa taactacaat accttctcga
      721 tctgctaatc gcataatttc ttctgagtaa ggataatgtg atgttctaaa agaattagca
      781 cctatccatt tcattaaatt aaaatcttta atatttacag cctcattcaa accacgtcca
      841 ttaacatacg aatcctcatg cttaccaaat cctttaaaat aaaaaggttt attattaatc
      901 aaaaatttgc cattggtaac ctgaacactg cgaataccaa attcttctgt ataggtatcg
      961 atcactttat tatcatcaat taaactaatt ttcatactat ataaataagc gtctagaggt
     1021 tcccaaaaaa ttggattctt aagctgaatc attcctttct ttccttcagc ttcagctaca
     1081 agttgagcct cttcgtcata tatcgctatt aagatttttc cggtccattc tgtatcgata
     1141 ttgaattcta cacttgcatc caaatcagtt attcttgtat taatagtaat atcacttata
     1201 tagtctttcg gagttgtata taacttaaca ggtctatgaa tacctgcata attgaagaaa
     1261 tcaaaattaa ctaagttttc cttttttata tccccatttt cctgtactgt ttccttcata
     1321 atccccacag gtaaagtact atcgtcaata atattgttta cagcaactgt aattcgatta
     1381 ttcccgtctt ttacaaaccg attaatttca gactcaaatg gtgtaaaccc acctttgtgt
     1441 tgagtaataa actttccatt caaaaaaact tttgcttcat gtgttgcaga tccaaaacga
     1501 agaactaatc tttcttctaa taatctttta ggaagagtca cttctttttc ataccacacc
     1561 catccaacat gatttctaat gttctctgtt tcaaataaat cattataaga agagggaact
     1621 gaaatcggat aactctcttt ttcatttatt ggttctaaag caagttcttc ctgtaagtca
     1681 tcatttgtat ctaatttaaa tctccagaga ccgcttaagt caattacttg cctcgtctct
     1741 gtcataatgg gatacaacat ataaaccact cctatctaaa tattaaataa atgtatcctt
     1801 taaaccacaa taagcatcta ttggacttac tcccttaaat tcagaaagac ccattagttt
     1861 gtccaccaat atttctaaag tttcttgttt tccatcataa gtattaatat aagtctttac
     1921 ttgaggtaca tcagccaaat gaaacggaca ctgtacagat acaaaaattg ttggaatttc
     1981 atgaatgtaa aacggtatat ccggagttcc tttacttgct ggccagacaa ttcgttgaac
     2041 tgtgcctgga ttaacattag ctatattaat gatcaagtca tattttttag ttaaattttt
     2101 aattggtctc ttagcagcat atattccagc tatattttta attttttctt cttgcgataa
     2161 tcctttcaat tcttcttcag gtgaaatgta tttttcaact tcaaatcctt ttgctgacaa
     2221 aatctgtttc aataaatcaa tggcttgagg tctatttcca gcaactactt caccaaaccc
     2281 accttttacc ccatgtacat ctactagtaa aatcctttta tatttttctg gtacaatagg
     2341 ccaaatatct tgtttatctt taactaatgt aattgcttgg tcagccacct ctttaaaaat
     2401 tgatttattt tcaattaaac caattttttc cagagcttct tgcttaggta ataaaatttg
     2461 ttcttttgct tttttgtgta atcccaattt agcttttaac cctaaaattc tttttaaggc
     2521 atctaataac cgatcatcag tgataattcc tttacgatag ccttccatca tataattaaa
     2581 gtcttcatct ggatcattaa aaaatagaaa tagatcacat ccagctgcaa tagaagtggg
     2641 caataattct gatcgtttca ttgccccagt cattcctaac atatgactag catcagtaac
     2701 tactaatcca ttaaatccga gttttcctct aagtaagtca gtgattaatt cttttgataa
     2761 agttgcagga agtaaatctt catctttcag atcaggatta aaatgttttg aataagctgg
     2821 catatggata tgacctgcca taattgaagg taatccagct tctattaagc ctgagtatac
     2881 ttttccaaaa gaattatccc attcttccgt acctaaacta ttaatactaa aagatagatg
     2941 ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta gctgcaggca aaattccact
     3001 ctcctgaata cctttcatat aagctaaact aagttctaaa actttatcag ggttttgtcc
     3061 aaaagatcga ctagaaatga taggatttct ccaatttcta ttaatatcta caataggagc
     3121 aaaactccag ttacatccaa tagccgatgc ttcaatccca gaaactctcc ctaattcata
     3181 agcatatttc gcatcatttg ttgcaccaat ttttacttct acaccaactt cagtaccatc
     3241 tgtacaagct ccgttaccac ctgcttccgt attagcagca attaatagag gaattttact
     3301 ttctgtttgt aagatatagt tttgatcata tacctcatca gcagttcctg gattatatcg
     3361 tacagctcca atatggtact gattgaccat attactcaag taatttttat cacgactaga
     3421 tcccatatta acaaacaatt gacctatttt ttcttccaaa gtcatagaat caatcgtctc
     3481 ttcaatccaa gaaatagcat cttcatctaa attatatggt ttttttcgta aatctacttt
     3541 tatagtcatt ttctttctcc tctacttatt agtaagattt ttccaaaaag tatctatata
     3601 tttttgttca atatctacat aatctcccga taaacattta aataaaacat cagttcttaa
     3661 gttttcccat gattcttttt ctttattgac caaaatagga taaaagaaaa ctttattttt
     3721 atttgcagct tctagatcac caggagaatc accaatcatt aatatcttat ccaaatcaca
     3781 accattttca attacacttc gaataatatc ttcttttttt cctttatctt gacaacacag
     3841 ttcatcaact aatgataata attcatgtct ttcccattct tcttgaacag cttccttatt
     3901 agctgagctt acaataatta tcttcccata ttttttcaga gcttttatag tatctctagc
     3961 acttaaaaag ggtttatcta atccttttgc ttgactaatc tgttgattga cttcttccga
     4021 ccattttaaa gcttttaata aatcctctga ctgagaatat tctatttccc tcttcaaaga
     4081 tggattagac aattcttttg ttagagatac ccagtgcttt aatcgattaa tcgatgttac
     4141 atcatttttt tctaatgttt taactaaacc taaaaatctg tttattccac gagtctctga
     4201 atataaatta atattattcc aatcctgtag gaatttttct ctatgatgaa catcaaaaat
     4261 ctctgcagca attggtccga aaaagagatt gtgtttataa gtcatcgtat ccataacaca
     4321 gccatctgaa tcaatacaaa atatccaatt attcatattt ctctcctatt tatgtttttg
     4381 ttttaaaagc tcgcaatttt ctaagacttt atctttagat aatggataaa ggaaaaataa
     4441 aattaatcct acaatcagaa aacaaattga tggaattaat gttgaagcgt tatataactt
     4501 ttctaaaaca tctcccgttt gtacatttgc taaagagtca tagcctataa tagaaagagt
     4561 ccaacctccg acgcctccag ctagagcttg acctattttt ctagcaaaag aatataccgc
     4621 atatacggtc ccatcttctc tttgatcaga ctttacttct tgatcatcaa taacatcagt
     4681 aatatttgcc caaataacag tattaaacac acccaaaccc atatagccaa ccgaactaag
     4741 aattaagaat acatacatat tactagcttt tatcaaaaat aagatcaaat atactattgc
     4801 tgaaaagaac attcctactg atgccagttc tttttttcca tattttttac ttaaaaacaa
     4861 agttaaaggc gaaacaacta ttaaaaccaa aataggattg ataaaattca ttaaaactat
     4921 tccctgtgca tcattatagt aatcaggaaa tacataatta ttcatactca taattaataa
     4981 ttgagcaagg agtaagaaaa tagccgcacc aattatagaa agtaatgccc tatttttaac
     5041 taaccctttt aaagatttta acaatgaaac tttgtattga gattggtcaa ttcttactcg
     5101 ttcagtagtt agtttataac atattaaata acaaataagt gcacatatcg aaaatattcc
     5161 cgcaacaatt gtaaaagttg atccaccacg aactatttga gcgcctgaat catctcttgt
     5221 ataaataaat aatggtgtta atacaccaat gaccaaactt gctaaagtag ctccaacatt
     5281 tcggaatgtc gatagcattg ttctttcttc tgtaatagaa gtaatagctg atgccattga
     5341 tccataagga atatttatag atgtgtaaaa aatactcccc cataatatat acgtaacata
     5401 catgtatact accttaacgc tcatagaagc tgaaatcata gaagtttgat acattaaaaa
     5461 acttgctaaa gcgactggac cactcattct taaaatccac ggtttaaact taccctgctt
     5521 agttgctttc gatctatcaa caatacgccc catagttaca tcagtaactg catcgataaa
     5581 tcttgctact aaaaataatg ttccaacggc agctccgctt atccccaata cttttgtata
     5641 aaagatcatt aaaaaagaac tagcaaaaat gaaagtaaaa tcgtttccaa aatctccaaa
     5701 catatatcct attttgtctc ttataccaaa ttttctttcc atacaattct ctcccttctg
     5761 ttgttaagcg ttttcattct gatgaaagta cgtactatac tctgaagcgt aaagagtcag
     5821 tatttatttt ttacatttca gagtattatt tgttttttct cctaataatt ctttatctca
     5881 taaaacttat ctatatattt actagctact cgacttactt cttcaaaatc acctttttca
     5941 acagaagcca ataaattccc acctacacca actgcaacaa ctcctacttc aaaccaatct
     6001 tctaagttct ctatacttac tccgccagta ggcattatat ttacgtgtgg taatggagct
     6061 tgataggctt ttacaatgga tggaccaaaa gcagaacctg ggaatagttt aatgatatct
     6121 acgccagcag taagtgcctc ctttatctca gtaattgtca tacaacctgg taaataagga
     6181 atttgatata aattacaaat ctcagctgtt tgtcgatcaa aagatggact aacgatatat
     6241 tctgccccag ccataattgc gatacgagca gtaaacgcat ctaaaaccgt accggctccg
     6301 attattacat ctttttgatc agcatatttt ttagcaattt ctttgattat ttcttcagca
     6361 ttaggaactg taaaagttag ttcaatccct ttaattccac ccgcaataat tgaatttatt
     6421 gctttaatgg cctcatcctt atttttccca cgtacaaccg caatgactcc agccgcttct
     6481 aacttactta aaatttctac ttttttcata tctatctcct tcctttatgg atacatactt
     6541 tgtctagctg tactaactta caaagaataa atttaagctt ttatttcaag tattattttc
     6601 tacttccgat ttcaaaaaaa ttaaaataat tatacgcatt acgataacaa atattctcca
     6661 ctatctgact aagatacatt tcatcatctg gaattcggcc tgctttaacc cactcgctta
     6721 gtaattgaca taatactcga cgaaaatact catggcgagg atagcttaga aaacttcgag
     6781 aatcagtcaa catacctgta aaattcgaca acaaactttg ttgtgccatc gtagtcaact
     6841 gttgacgcat tccttcagct gtatcattga accaccaagc acatcccaat tggatttttt
     6901 gaatcatacc cccttggaag ctttgcattg ctgttgctag tggaagccaa tcagttggat
     6961 ttaatgaata taaaattgtt tttggtaagc acccactctt ttcagcggta tcgaacaatg
     7021 ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga atcaccaccg acatctactc
     7081 caatttgttc ctttaatata cttgaattat ttcttaaagc atttaaatgc atttgcatcg
     7141 tccaaccata tcttgtatac tcagacatca aatgtaattg aatacctgta acaaattgtg
     7201 caatttctga taaatggaaa tccgtttttc ctgctaaagc ttttattaac agattgtcga
     7261 gtattttttc atctgctatt tcataaaaat aagtattcaa accgtgatct gctaatcgac
     7321 agccaatttg atgaaaatga tccactcgtt ggctcaatgc ttgcttcaac ccattaaagt
     7381 ctttgattgg tatagccgct gtttgactaa gttttaaaat atagttcgaa aagcctgatg
     7441 atttgatact ccagacctta tctggacgga aagtaggtaa taccttgaaa gaattatcct
     7501 gtttagaaag ctcagtatga taccacaagt catctgtagg atcatccgtt gtacagatgg
     7561 ctttcacatc catcatctcc aacaattctt tcgggcgata ggaatccgtt tgaatttttt
     7621 cgttagcacg tcgccaaatt tctggtgcat tcttctcaga aattgtcaaa tcaatatcaa
     7681 agtaacgttg taattccaaa tgagaccatt caaaaatagg attacctggc gccttctcca
     7741 ctgtttttac aaaagctaaa aatttttcat aatcatcacc tggaccactg atcaactctt
     7801 cttctatacc attagctcgc atcaatcgcc atttataatg atctccagct cctttatcat
     7861 ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc tagcaaatat gctgggtcta
     7921 aatgacaatg atagtcgata attggttggt gttttgcaaa tttatgaaaa agtcttttag
     7981 ctatttcatt ctctaataaa aagttcttat ctaaaaacat atttaaacac tcctttcctt
     8041 ccgttagttt ttagaagaga aaatttttcc tctaaataaa ctcccttata taattattta
     8101 ctcgccccct tttcaagcgc ttcccaaata ccatttaaat aaactgcacc tagagctcgg
     8161 tcatataatc cataaccagg tctaccggtt tctccccaaa tcatccgacc atgatctgga
     8221 cgaatatagc cattaaatcc attatcgtac attgttttta atatcttata catatctaga
     8281 gaaccatatt ctgatttatg cggcgcttcg taaaaatcga gtccattgtc acttaaatat
     8341 ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt gacaaaattc agatagaatt
     8401 tcatagacat cattatctgg tcgttctgca atacttccag tacataatgt aataccatta
     8461 gaaggagaat ccactacatt acacaaccaa tctaaatcgt cacgattttt aacaatacga
     8521 ggtaatccga aaatggaata tggtggatca tctgggtgaa ctgccatttt aatcccaact
     8581 tcttcacatg tcgggataat tgctttgaga aaatatgcaa agttctctcg taatttctct
     8641 tcatcaactt ctttataggc ttctaataat tgcttaattt gtgataaccg atgaggttcc
     8701 caacccggca attcaaaatt attagtagaa ttttctactt ctctaataat ttcctccgga
     8761 tcagtaggga tattttttga aatataggct aacgtagaag acccatcatc taaacgataa
     8821 tccaaatccg attttaccca atcaaatact ggcataaaat tataacagat aacctcaatc
     8881 ccaaattctg ccaaattgcg aatcgtctgt ttataattct caatgtattg atctcgtgtt
     8941 ggtaaaccaa ttttgatatc atcatgaaca ttaacactct caataacttt caaggtaagt
     9001 ccattggctt cgacttcatc ttttaaagat ttaattcgtt ctctcggcca aacctctccg
     9061 gctggtatat ccattaatgt tccaacaatt cctttcattg ctgggatctg acgaatttct
     9121 tgtagtgaga ttttttcttc atttgcccca taccatctaa aagtcatttc catagttact
     9181 ttcctccaac ttctgttata tatttattga ttgttttacg aactgcttga ggacctacta
     9241 gcatttcgca aaagattttt tcagttttct ttcctacccc aacttgatat aaatctaaac
     9301 caaagatttc tttattttct aaaatttcct gtaccgcaca atgaatcttt gaaggatctt
     9361 gttcacctaa tttcaatact gataatttat cctgcaactc taccaataac ggatcaggac
     9421 ttggcacaaa attttcccct tgatcatcga ctgccagtaa ataacgtaac caagcagcaa
     9481 tagcgatagg aataaatgtt agattttctg catttccgtc tttttcgaca tactttttaa
     9541 ttgtttcacc atagcgaatc gctacttttt gagaagtatc tgttgcaatt cgttgtggtg
     9601 tatctggaat atagggatta ggcaaacgtt tattaattac ttcatcaata aattgttttg
     9661 gatcaataat gcctggattt tcaactactg gtaaaccttc taaataacct attcctttga
     9721 ttaaaccaac caaatcatca tcctgcatct ctgctgaaat tgattgatat cctagcagac
     9781 aacctgttac tgccaaagca gtatgaagtg gattcaagct tgtcgtaacc ttcatgatgt
     9841 ctgctttatc aaccgtctct ctatctgtca taatcacacc agccttttcc aatttaggcc
     9901 ggccatttgg aaaactatct tcaataacta aataatgaac tacctcagta ttagtgaaag
     9961 cagcaatatt ggtatgtttt tttgtgtgaa tgatatctaa atcctcgagc cccatcatca
    10021 atagatcttc ctttacattc tctgaaggat ttggcgtaat tcgatcaatc atactccaag
    10081 gaaaagtcac tttttcttta tcctttaaat aagcaataaa tttttcagaa acatatcctt
    10141 ctttttgcca agcttctgca atcgtaaaaa tactatcttg aaaacgttga ccattctgtg
    10201 agaaattatc agtagaaacc atcgcaatcg gcaactctcc agcattaaat ctgctatata
    10261 acaaggatgt tacaatactc atcgtatgtt gaggattgct aggtccagat ttgatatcag
    10321 ctttgactac cgaaaagaat tgccctttag aatcttttaa actgtaacct ttttcagtaa
    10381 tagtaaacgt tgtaaactgt aatttaggat cttcaaaata tttaaccact ctttgatagt
    10441 ccgctatatt cttcggattg caatataaac tatcagccgt tactgccaaa agtctttttt
    10501 tgagttcacc atctccactc ataacaattt ccaaaatgtc attgttatag tcatgataag
    10561 ctttttccac tacttcttca tcaaatgttt cacacacaac tactcctgtt tctaattcat
    10621 tttgatctgc taagcattga gcaatttctg catgaaatcc tcgataaaga tttcctccac
    10681 caaaatgaag ccatactgga tgttgctggc cttcttgctt aacttctttg acatcaaaac
    10741 acggcagtag aatctgcatt tcattgaatt cttcgataga attttgccaa tttttaatcg
    10801 aaaccataat taacctcctc aaactgatat gttagttttg atacatttta tattttaaaa
    10861 cccattgtaa ccgttgtcaa tataaaaagg gaataaactt gcttttttct accatatcag
    10921 aatgattgta ttttttattc agaaaaataa aatactcctt ctttttggac tgattttatc
    10981 gaaacttatt ttttcttttt ttcatgtatt cttttatttt tataactagt gtattacttc
    11041 aaaatttgat attatatggc atgaacagaa ttaaaatatc agttaaaata aaagttaagg
    11101 agcattgtgt atgacaatta aaactagtct ccaaaatcaa gcttaccaat ctattcgccg
    11161 acaaattatc tatgctgact tagaacctgg aaaaaaagta tcagaaaaaa aattagaaga
    11221 aacactttct attggtcgta caccaatacg tgaagcactg attcaactcc gacaacaagg
    11281 tcttgttgat acaattcctc aaagcggaaa ttatatctca ttaattgatt tagattcggc
    11341 caaaaatgct cgatttgtca gagaacattt ggaacgacaa attatgttag agtgttgtgc
    11401 caaaatgaat aaccaaaaaa aacggatttt agaaactata atttcagaac aagaaaaagc
    11461 tgttagtcaa aaaaatgctc gtagtttttt tcataatgat aatttattcc ataaagcttg
    11521 ttttgaaatt gcagagcgag aagaagtatg gcaatggtta gaaaataaca atacacactt
    11581 agaacgattt cgatggctac gagtaaaaac ggaaggatta aagtgggaaa aaattatgga
    11641 acaacattat caactatttg aggcattaac aaaacaagat ccagaagaag ctaactttct
    11701 aacatcgttg catcttcatt taatgcttaa tgagcaaaat attgttttag ctactcatcc
    11761 aaaatatttc aaaaatatat aacttttaac tctttttaaa ttaaatggtt gttctgatca
    11821 gaaaaatatc gcttgttttc ttattatgta atatatttca taaactattc tgcataacaa
    11881 ctattgtttg tactttttac attggataac taattatgac tgctgataac ttttgatatt
    11941 aaactagtat ctccaataca aataaaaaat agatatcttg ttcatcaaaa ttagttcgtg
    12001 gatacttacc ttcatttaca tcattcagcg gtttcccatt ttcttttatt ctattatttc
    12061 attttctaat agacttggct tcatttcgca atactttttt tcaataggca tcaaattggt
    12121 gttgtttaga tatttagttg tagttttgtg acatgataag tatgctcctt tttctctttt
    12181 ccactaatac aacgatcaaa gggtgctttt ttcaaaaaac tagaaaaatc aaaataagat
    12241 tcgtattttg attctatata tcctgtttca actacatctt atatttcaca atagaataaa
    12301 tagccttttg catagctgct taacttataa gaaattgcaa tttacttctt caaagtgaaa
    12361 acgaatatga gtaaaataaa attattcttc ttacagataa ttaaaaatag cgcgtaacaa
    12421 aaatcggcat tcaatcacat aattaggatt gatgccgatt acttatattc tacgtatagt
    12481 tttagtattc ttatttttta tcaccagtaa ccaattgaga acagccctaa agcagaatag
    12541 tcttttatct caaaacgctt aatttcaggc ttaggaatat gaataatctt ttgagtatat
    12601 tctttctctt ttaatcttac tgaagaaata ccactgccta tcttttttac aaatatttca
    12661 tttggtgtac agactaataa aacaatattt gtaatcaatc cttgcactaa agaattaaaa
    12721 ggaagactat
//
LOCUS       DASJSZ010000077        12174 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.081, whole genome shotgun sequence.
ACCESSION   DASJSZ010000077 DASJSZ010000000
VERSION     DASJSZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.081"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..610
                     /locus_tag="U4B03_002175"
     CDS             <1..610
                     /locus_tag="U4B03_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2069000.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="U4B03_002176"
     CDS             723..1001
                     /locus_tag="U4B03_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN2069001.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="U4B03_002177"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="U4B03_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEN2069002.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="U4B03_002178"
     CDS             1458..2213
                     /locus_tag="U4B03_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2069003.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="U4B03_002179"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="U4B03_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEN2069004.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="U4B03_002180"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="U4B03_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEN2069005.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="U4B03_002181"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="U4B03_002181"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN2069006.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="U4B03_002182"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="U4B03_002182"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEN2069007.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="U4B03_002183"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="U4B03_002183"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEN2069008.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="U4B03_002184"
     CDS             6950..7492
                     /locus_tag="U4B03_002184"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN2069009.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /locus_tag="U4B03_002185"
     CDS             7515..9071
                     /locus_tag="U4B03_002185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN2069010.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="U4B03_002186"
     CDS             9085..9987
                     /locus_tag="U4B03_002186"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN2069011.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="U4B03_002187"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="U4B03_002187"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN2069012.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="U4B03_002188"
     CDS             11430..11852
                     /locus_tag="U4B03_002188"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN2069013.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4046 a         2159 c         2687 g         3282 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatttgggc tctttgtcaa atgatgttgg taaagagaaa tctt
//
LOCUS       DASJSZ010000078        12162 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.082, whole genome shotgun sequence.
ACCESSION   DASJSZ010000078 DASJSZ010000000
VERSION     DASJSZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.082"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(102..185)
                     /locus_tag="U4B03_002189"
     tRNA            complement(102..185)
                     /locus_tag="U4B03_002189"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(149..151),aa:Leu,seq:cag)
     gene            713..1213
                     /locus_tag="U4B03_002190"
     CDS             713..1213
                     /locus_tag="U4B03_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2069014.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1403..1747)
                     /locus_tag="U4B03_002191"
     CDS             complement(1403..1747)
                     /locus_tag="U4B03_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEN2069015.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1901..2821)
                     /locus_tag="U4B03_002192"
     CDS             complement(1901..2821)
                     /locus_tag="U4B03_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2069016.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3008..3499
                     /locus_tag="U4B03_002193"
     CDS             3008..3499
                     /locus_tag="U4B03_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEN2069017.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3523..5898)
                     /locus_tag="U4B03_002194"
     CDS             complement(3523..5898)
                     /locus_tag="U4B03_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN2069018.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6224..6850
                     /locus_tag="U4B03_002195"
     CDS             6224..6850
                     /locus_tag="U4B03_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2069019.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6873..7577
                     /locus_tag="U4B03_002196"
     CDS             6873..7577
                     /locus_tag="U4B03_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2069020.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7769..8602
                     /locus_tag="U4B03_002197"
     CDS             7769..8602
                     /locus_tag="U4B03_002197"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEN2069021.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            8799..10727
                     /locus_tag="U4B03_002198"
     CDS             8799..10727
                     /locus_tag="U4B03_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN2069022.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            10865..11320
                     /locus_tag="U4B03_002199"
     CDS             10865..11320
                     /locus_tag="U4B03_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEN2069023.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            11790..12059
                     /locus_tag="U4B03_002200"
     CDS             11790..12059
                     /locus_tag="U4B03_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069024.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
BASE COUNT         3715 a         2271 c         2217 g         3959 t
ORIGIN      
        1 tggttccgtt gtttaaaatt tttcctatat tcttttagat aatatgttat tttctttctc
       61 atcgctccag gtgtaccttc tctttataaa aaaacaaatt atgcggctaa gaagactcga
      121 acttccacga ggtcaccctc actagcgcct gaagctagcg cgtctgccaa ttccgccata
      181 gccgcatcac atcttttagt ttaggattac atgtgttttt tgtcaagaac atatttttgt
      241 tctttttcat ttctgtacgc cctcccttat gccatctaca caaataattc atccgagcaa
      301 ctccatcatc tattgagggt tggtgaaaaa atctgtttta tccatatgat cgatttcaat
      361 gttaaacgaa cgactctggg attagtaact attttatttt tttcttcatt tcttttttcc
      421 tctttttctc gattcagcta tcttctttca tttggatatt gattatttct tctgttgatt
      481 actatccttc tggaattcta gacgctgaag taattcacgt tttctttctt ctagagatca
      541 taagtaacga atgaacgaac ttcaaataat ttttttattg taattcatat ttgtattgtt
      601 cgatgattct taatacgata tgacacaata aataaaaata ggcatggcta tttacaacaa
      661 tagtgctata gtagaaaaca aatgaataag gttacttaaa gaggtgaata cgatgaaact
      721 gatcaaacta tgggaagcag atttaaagaa agcttatcag ctacaaacta gctttggacc
      781 aaatgagagt ggatttatca acaatgctta tggctataca tttgctgaat ttttagatta
      841 tgtaaaaaaa tgcgaaaaaa cgtctttagg aatcgatctt cctgagagtt acgttcccgc
      901 aaccgtcttt attttagaaa gtgatgaaca tgagtatgta ggaattttta atttgcgcca
      961 taaactgaac gaaacattaa gaaacggcgc tggccatatt ggttacggca tctcccctaa
     1021 ttatcgtaaa aaaggatatg ccacacaagg attagcatta gtactacaag aggcaaaaaa
     1081 aatcggcatt gatgaggcac ttttgtctgt aaataaaaaa aatcttggca gtcttaaagc
     1141 acaacaaaaa aatggggctg cgatttatcg tgaagatgag aaagaatact atacgaaaat
     1201 caaactgatg taaaaaagaa ggattccaaa aggaaaggga tttgaatgtc ctttatcaat
     1261 agaacacatt tatttaatta acttataata tagcagacaa aattttataa acaaagaaac
     1321 cttttatctc ttttcttcta gaaaggataa aaggcttttt tgtttttcct atttgcttct
     1381 catagctagt cagccaagat ttctatcgta tccccaacgc aatccgtgca taacggctca
     1441 tcttatctgt cgtccatgct ggataccaga ctagtttgac ttctgctttc gttacttctg
     1501 gcacttcttt taatgcgtca tggatagatt ctgttaaaat atctgctaat ggacagccca
     1561 tagttgttag agtcatctta atgactgttt ctccattttc aggattgaat tctatatcat
     1621 aaatcaagcc taaattgacg atgtcgattc ctaattcagg atcaatcacc atttccaagg
     1681 cagcaagaat acgttctttg attagttcta tttcttgtcc agaccgttct tggtttgctt
     1741 cgctcatcgt acgacactcc ttaacacgat ttttatttat catactcttt ttttagctgc
     1801 ttgtaaatga gaatttagaa gtaagattct cgtttaaatt tataaaaatg ttcttattag
     1861 atgtaataga ttaaatttat tctaaaataa acctgattcc tcattttttt tgtgaacgat
     1921 tttttatttc tggcaatacg ccagttgttc gatacatttt ttgttcgttt tcatttgcaa
     1981 accggccaaa tactgaatga gttggtaaaa tattgataaa tgcatcgtcg tcgatttcat
     2041 gaacgatctg atcaagatca tacatttcgt agcgggtgat gaccatcatg atcatgttcc
     2101 cttgtttttt tgaatagcca ccgacagacg gcagtaatgt cattccacgt accatccgtt
     2161 ttgctagttc ttctgttacc tcttctggct tgacggtaac gatcattgct gttacttttt
     2221 gatgactcgt gtggatggca tcaattactt gagtcattgc ataaatggaa ataatcgtat
     2281 acaaggcact ttcccagtta aagaagaagc ctgcgatgac tacaatgata ccatttagaa
     2341 tcagcatgta gtttccgact gtttttccag ttgtttgtga aagtacaaga gagatgatat
     2401 ccatcccacc agttgtaaag cccattttta aagataatcc tacgccaaga cctaataaaa
     2461 caccgcctac tagggcattc atcaaaatat tatcagtgat ctgtccgact ggaagcagca
     2521 tcgtcatcac cgatacacat acaacattca caaaactaag gatcgttgct cgttttccta
     2581 ctttgagaaa ccctaataca aacacaggga tattcaaaag aaaaataaat gttcctgtgt
     2641 ctatcttgat atgaaaccat tcatataaaa gcgaggaaat gatttgagcg atcccattca
     2701 tccctgcaga aaagacattg gctggaatca aaaaataatt taaagcgacc gcagcggtca
     2761 atccggttat caaaataacc actattttct ttaatctttc atttttatga tacgcagtca
     2821 taattttact cacaagcacc tctcccctac tttggtattc caaatgtaag aataacagtt
     2881 tatctattaa aataaaatac ttcgaactca aatgagaaga attttagcaa acaggtaaga
     2941 atctgcccta atcctatgat aagatagatg tatggtaaat atttatatgt atatcaggag
     3001 ggttcctatg gcaaaagatg ctagcttcga cattgtatca gaagtcaata cagaagaagt
     3061 aaaaaatgcg attcagcaaa caacaaaaga attgtccaat cgctttgact tcaaggattc
     3121 tacagtagaa atcaaattag agaacagcaa tctagttgta ttgagcgatg atgatttcaa
     3181 aatcgaacag atcaaagatg tattatttgg taagctgatc aaaagaaatg tgccaatcaa
     3241 aaatatccat ttttccgaat cagaacacgc tctaggtggc aaagctcgac aaactgccga
     3301 tttggtcaac ggaatcgatc gtgaaaacgc gaagaaaatc actacagcta tcaaaaatga
     3361 aaaactgaaa gtaaaagcac aaatccaaga agatcagatt agagtgacag gtaagaaccg
     3421 tgatgatcta caagctgtga ttgctttatt acgtaaactt gacttaccaa tcgagttgca
     3481 attcactaac tatcgttaat caaaaaacga ggctgacgtc tgtcattcct cgtttttttg
     3541 atttactgct tcttttgata accaaaaacg ctgtattcct tcttccaatt ccccaacact
     3601 tcgtacttct cttgctgttt gccaatgttg tgggaataaa cgtcgataag aaaaactaga
     3661 aaatcgttga tctgccaata caacgattcc ttgatccgcc atgccacgaa tcactcgacc
     3721 accagcttgc aagactttgt tcattccagg aagctgataa gcgtaagcaa atccaaggtg
     3781 ttccttctca tcataatatg atttgatcag ttcctgctca tgattcatct gcggcaaacc
     3841 tacacctacg atcatactgc cgatcaatcg gtcccctctc aaatcgattc cttctgaaaa
     3901 aatcccgcca agtacgcaaa atccaatcaa tgtttcagcg ggatttgttt gaaattttgt
     3961 taaaaatcgt tcgcgttctt cctcattcat gtccgtttct tggattagca cattgctgtc
     4021 gggatattgc tgtctgaatg cttccaccgc aagatctaaa tattgataag aaggaaagaa
     4081 aacgaaataa ttcccgacct ttccatcagc aaaagcatga atcgtctcta ctaattttgc
     4141 cagactgtgt tccctttttc gataagtcgt ctctaggtaa tttgcgatga aaacttgctg
     4201 gtttccttct gggaaaggac tcggcagttt gtaagccaga cttttttgtc cccccagtgt
     4261 ttcctgataa taagagagtg gtgaaaaact agcagaaaaa agaatactgc tttttccttt
     4321 atccaacgac tgctcaagaa acaagcttgg atcaatacaa aactctttta ctatcagatc
     4381 gcgatacgtt ttttcgactg ttgtttcgta gtgatcatca taaaattcac tgaccttcaa
     4441 aaaatgaagc aaatcaaaat aataaggaag caattgttct tgtgatgggt gctcaggaaa
     4501 ttctgccagc cattctttaa ttttttcgct taattgataa cctgccttta ctaaagattc
     4561 agccggtgct ttttgatgat gataatccca atgatcttct tttgctattt ctcggatcga
     4621 atcaaattct tttaatagtt tgttgaaacg gcgatgaagt tttttgaatt cctttggtat
     4681 agcttcctta cttcgtttcg ttttttcata agatagttct gccgaataca tctctcggga
     4741 acgattcacc aaattatgtg cttcgtcaat caaaaacaga tagtcctcgt ttttttcatc
     4801 ttcaaaaaaa cgtcttaaat aaacagttgg atcaaataaa taattgtaat ccccaatgat
     4861 tacgtcacac cagatactga catctaatga tagttcgaaa ggacaaagtg tatgctttct
     4921 tgcataagtt tcgatgactt cacgagtgat ctgattctca tgattcaaaa gatcccataa
     4981 accttcatta atccgattgt aataaccatt ggcatatggg cactgatctg gattacaggt
     5041 cgtctcatct aaaaagcaga tcttgtcttt cgccgtcaaa gtgacacttt ttacttcagc
     5101 tcctgtatct tttaatgctg tcatagcatc ttcagctact tgtctagtta ttgttttagc
     5161 cgttagataa aagatctttt ctccctcttc ttcccctaca gctttcaaag cggggaataa
     5221 cgtagagatt gtttttcctg ttccggtagg tgcttctaca aacaatttct gtttcgtacg
     5281 gatcgtttta taagcagcta ccgctagttc acgctgacct ttcctatact catcatgggg
     5341 gaatttcaaa gccatcaaag aggcattgcg tacagtgcgc cagttttctt gaaaaacaag
     5401 ccattcttta taatcttcag tcagcttttt aaaaaaagtt tctaactgct ctcttgtctg
     5461 atgttcaacc tttctcgtga tcacttcttc tgccgtctgg aagtaagtca attgcagatt
     5521 tatttcttgt aaatcatgtt cttgactata gatataggca tacacccttg cttgatgaaa
     5581 gaaaagttcc acttgttctg gttctaaatc ttcaaatcgt ggttcggagg ttttgatttc
     5641 atctatataa taaataccgt ctcttttgaa aataccgtct gcacggcctt caacgatcag
     5701 gtaatattca tctaattgaa ctgttgtttt taaaaagact tctttttcat agttttctcc
     5761 cgcatctttt tggagcttgc ggtggattct cgctccttct aatgcagtat ggttactttt
     5821 tttacgattg tcgatacttc cacgtcgcaa aataaattct actaagtgcc gcacagatat
     5881 ttttgactgt ttgatcattt ccaccacctc tttctctcta agattacctg aaaaaaccta
     5941 gtttagcaaa aagagctctc tttccaaaca taaaaaaaca ccctgctgat taggcaggga
     6001 tataggatat agaatttgga tttttggatg aatctaatgt aacaaggaca tattaaaatg
     6061 ctatgaaaac ttactttcct tctctttaga atatccaaat acttttcata tagtctgata
     6121 attcagataa gtaaagagaa ataagtacct gtttttgttt cctagcaatg aatgcgttat
     6181 actaatggat gaaagaagtt ttggctatca aggaggaatg cacatgcata tcgaaagatt
     6241 aaaaacagga atgattgaag aaaactgtta tttggtctac aacgaagaag cacttttgat
     6301 cattgaccct ggagaagatg cggaacgaat caaacaagaa atcaaaaaaa caaaccaaca
     6361 gccggtagcg attttgctta cccatactca ctacgatcat atcggtgcag tggaaccttt
     6421 acgcgcgtat tatcaaatcc cagtttatgt cagcccacta gagcaaaaat ggctaggtga
     6481 tccgattttg aatctttctg gactcggtag acatgatgat attgctgatg ttattgtctc
     6541 gcctgcagaa tatgaatttg aaatgaaaac ttatacttta ggaaacatgt cttttcgcgt
     6601 cgtccctacc ccagggcatt ctataggaag cgtcagcttt atttttgatg attttgtcgt
     6661 ttctggcgac gccctgttta gaggaagtat cggtagaacc gatctgtata cagggaattt
     6721 agaacaatta cttcatagta ttcggaccca gctgtttgta ttacctgatg aatttgcagt
     6781 ctatccagga catggcgatg caacgacgat tgaacaggaa aaaaggacaa atcctttttt
     6841 caatcgttaa aacgacttag gaggagaata aaatgacaga aactacactt tatatcgtac
     6901 gacacggaaa aacgatgttc aatacaattg aacgagtaca aggctggtgt gatacacccc
     6961 tcacaaagca aggacaagaa ggcattcatt atttaggtcg cgggttaaaa aatgtcgact
     7021 tttcatttgc ttattctagt gacagtggtc gtgcaatcga aactgcccgt attattctag
     7081 gagaacattc taagggaaaa gaaattcctt attttattga aaaaaggatt agagaatggt
     7141 gtttcgggtc tcttgaaggt ggatatgaca tggaaatgtg gggtgtgatc cctcgtgtat
     7201 tgaattttaa aacatatgat gaaatgttta ccacagaagt ttcttttgaa caaattgcta
     7261 atgctgtata caacttagat accgctgggt gggcggaaac ttatgaaaca ttgcgagacc
     7321 gtgtatggac aggatttgaa gatatcgccc atcactgcga aaaaaatggt ggtggaaatg
     7381 ttttagtcgt atctcatggt ttgacgatcg cctttttgct taatctgatc gatcctaaac
     7441 agcctgttcg tgctgggctt ttaaatggca gcgtaacaaa ggtcacttat gcaaacggtc
     7501 gattttctat ccacggcatc aatgatacgt cttatatcga taacgggaaa acacatagtc
     7561 gcgactcgtt tctataatgg ggatatttta atttttacca gagaatcata aaaggcaaag
     7621 tctgaacgag ggctttgtct tcttttttct cttcgtgtac ttatctgctt tatttctcaa
     7681 gtttttctta atattaaaaa acagattggg tttctcaaaa aataacggta tactataaga
     7741 tgtatcttaa ttgggaagga aggaatttat gttttttgca aatattatca aagcagtgat
     7801 tctcggtatt attgaaggaa taacggaatg gctgcccatc agtagtactg gtcatttgat
     7861 cctagcagat gaattcatca aactggatgc cagttcacaa tttatgtcga tgttcaatgt
     7921 cgttatccaa ttaggggcga tcttggctgt cgtcgttctt tattttcaca agttaaaccc
     7981 attttctcca agtaaatcaa cggtagaaaa aaaagatacg tggacacttt ggtttaaagt
     8041 gatcgttgct tgcataccag ccatcatcat tggtttgcct ttggatgatt ggttagaagc
     8101 aaatttccac aagttcttac cagttgccat tgttttgatc gtttacggta ttgcctttat
     8161 tattgtcgaa aaacgcaaca aaaacaaaac acctcgttgg acttctctga atgactttac
     8221 gtaccaagca gctcttcttg ttgggttatt ccaagtcttg gcacttgttc ccggaacatc
     8281 ccgttctggc gcaacgatcc taggatctat cttgattggt gcttcgcgtt ttgtcgctac
     8341 agagttttca tttttcttag ggattcctgt catgtttggc gcaagcggct tgaagatcgt
     8401 caaatattta gctgaaggga attcattcaa aatggaagaa actgttgttc ttcttgtagg
     8461 aacattcgta tcttttgtgg tttccgtctt agctatcaaa ttcttactaa gctatttgaa
     8521 gaaaaacgac tttaccgctt ttggctggta tcgtattatc cttggtatca ttttgattgg
     8581 ttattggttt attgcaatgt agaaaaaacc agaacacttc tcttatttga gaaatgttct
     8641 ggttttttat tttctttaat aataatcatt ataaataata aaaacaacat caagcttaat
     8701 caccaaatga taaccattat cgttctcatt tagctgttat tattatcaat tacattttta
     8761 ttatgctatc cttgttttga caaggaggtc taataacgat gactaatcag aaaaaagctt
     8821 ttgcagcaac aatcggctgc cttttattta tgatcttcgg ttttatcttc gaccgattct
     8881 ctcttaccat caccccactt ttttatatca gtgccattat catcggtgga tataaacaaa
     8941 catcggaagg cttgaaagaa ttgtggaatg atcggacact gaatgtggat ttattgatgg
     9001 ctttagctgc tattggtgcc tgtttgatcg gtaattactt tgaaggagca atgttgacat
     9061 tcatcttttg tttaagcggt gcattagagg aatacacaac taataaaagt caaaaagaaa
     9121 ttactgcttt gatgaatctt caaccacaaa aagcccaatt attaaaagaa aatggacaaa
     9181 tgcaagaagt agatgtttct caactaaagg tggataactt ggtatttgtc gctaaaggag
     9241 cagctgttcc gatagatggc tttttagaaa gcacacaggc aacgatcgat gagtctgcat
     9301 taagcggtga atctgtgcct gtagaaaaat atttaggcga tgagatgtat gcaggaacgc
     9361 tgaatcaagg aaatcccatc acgattcgag taacaaaaac gagttccgaa actgtctttg
     9421 ctaagattat ccaattagta gaagaagcgc agaatacgcc aactcaaaca gcttcattta
     9481 tcgaacgaat cgaaaataat tatgtaaaat tgattttgct tgctgttcct ctaatgatcc
     9541 ttctccccca ctttttccta ggttggagtt gggatgaaag cttttatcga ggaatggtat
     9601 tattagttgt ggcttctcct tgtgcgcttg tcgcatctgc tacaccagct tctctagctg
     9661 ctctttcaaa ttctgcaaaa aatgggattt taatcaaagg cggtatccat ttagaaaagc
     9721 tttctgaatt aaaagcgatt gcctttgata aaacaggaac gctcactaaa ggaaaaccag
     9781 ttgtaactga cagctttttc tttgatgaaa aggatttggc acaacaactt cttgttgcta
     9841 tggaacaaaa aacgacgcat ccacttgctc atgcgatttt gcaatatttc gattgcacaa
     9901 tacctgaaga aatccaatac ttaccaatag aagaaattac tggttttgga ttacaaacta
     9961 cctatttagg tgcgcaatgg aaagttggca agcacgcata tgatcctgag acgatgataa
    10021 tcagcaaaga gattgcggaa atgatcgaac gtttggaaaa tcaaggcaaa accgtgattt
    10081 atctttctaa agatcagcag ttaattgctg ttttaggtct gctagatatt ccaaaagcaa
    10141 atacccaaca agtaatttcc tactttaaat cccaaaacat ccatacaagt atgataaccg
    10201 gcgaccattc tggcacagca aaggccattg ctgaacaagt agggattgaa cattactatg
    10261 caagctgtac tccggaagaa aaaacacaac tagtcaaaaa agagaatgaa ctttaccaag
    10321 taaatgcgat ggttggtgat ggagtcaatg atgcgcctgc tttagcagcc gcttctttag
    10381 gtgtcgctat ggggcaaggg acagatgccg ctatggatat cgcagacatc gtcttaatga
    10441 aaagcgactt aaataaattt gtctacagtc atcggctatc tttaaaaatg cggcggatca
    10501 tcaaacaaaa tattattttt tcgattcttg ttattctctt gttgattgct tctaacttcc
    10561 tgcaattttt aaatctacct cttggagttg taggacacga aggaagcaca atattggtta
    10621 ttctgaatgg cttgcgttta ctaagacctt tacctgatct ttctgattct tcaaaaaaat
    10681 gtaagtcttg tccactatac aaagcgcggc tgtcttctgt caaataagca aagtgttttt
    10741 ttatgatttt gtttatttta tgaaaaaaac agcttatgaa taggtcttga aggatgaatt
    10801 cctacttttg taactttcta aaaacatgtt atgattgttt cgaagaaagt gaggaatata
    10861 tttcatggaa agttggcttt ttttaggaat tattttactt gtagcaattt tagctaaaaa
    10921 tcaaagtctg atgatagcgg cagcttttgt tttgattatg aagctcatac cagcctcccg
    10981 ttcctttttc ccttggcttc aaggcaaagg aatcaatatt ggggtgacga tcatcacagc
    11041 ggctattcta atccctatcg caacaggaga aatcggtcta cgtgatctat tagaagcctt
    11101 taaatcccct attggctggg tagctgtttt ttgcggtggg ctagtggctg tcttatcttc
    11161 caagggtgta ggattgattg cttctgatcc acaaatcact gttgcacttg ttttcggcac
    11221 gattttaggt gttgtcttct taagaggaat cgccgccgga ccggtcattg cttctggtat
    11281 cacttactgt atcttacaag tatttgcttt aggaaaatag aacaaatttt gtctatttta
    11341 ggagattctt aacaccccca atttactgat ccgtgatata ctgacaacga ggtgatacaa
    11401 tgttcgaaaa attaaagtat ttcataggaa caatcggctg gtttcttcta gcggtacttt
    11461 tgattctggg cttagcttat ctattatttc ctgatttgat acaaaatgtg tttgatactg
    11521 ctttttgatt gacctattaa aacgatatag gggatgctcc tgcgatgcag gggctttttt
    11581 taatcaatgt tttgtaataa tttaggccgt gccaatgaga cttcctatag aattcgatgc
    11641 tcttttttct ttttggctct tctgctcttt tttgttatac tagattacag tggacaaaag
    11701 aaagaatgat cgcatccact tcaacgaata tcaaaatgga agaattagtg cctgttttca
    11761 ggttactttg aaaagaaagg aagacgcttg tggaaacttt tgaaggtctt ttaattgata
    11821 cgtacgtttt acaagaacag ccaatgatgc tgactgggat caacttgcag cttgaggacc
    11881 gtgtgatcaa tggcctgctt aaagatcgct atttggcaaa atatatctta cgtttgcccc
    11941 taaataaata tgtgctggaa gtaagtggca aatggaaccg tcggaaccag ttggtcataa
    12001 aaaaaatgtt gataaaaaat atcgatgaat acattaaaat aatcggtaca ccagaatagc
    12061 ctttactgat caaaggatac tggttataaa ataactgcag tttcttaata gaaaaacaaa
    12121 gaacaacagg gaatcgtatc ccttttcaaa aaaaactttg ca
//
LOCUS       DASJSZ010000079        11994 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.083, whole genome shotgun sequence.
ACCESSION   DASJSZ010000079 DASJSZ010000000
VERSION     DASJSZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.083"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..330)
                     /locus_tag="U4B03_002201"
     CDS             complement(1..330)
                     /locus_tag="U4B03_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEN2069025.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            507..1235
                     /gene="sfsA"
                     /locus_tag="U4B03_002202"
     CDS             507..1235
                     /gene="sfsA"
                     /locus_tag="U4B03_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEN2069026.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1251..2264
                     /locus_tag="U4B03_002203"
     CDS             1251..2264
                     /locus_tag="U4B03_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2069027.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2264..3541
                     /locus_tag="U4B03_002204"
     CDS             2264..3541
                     /locus_tag="U4B03_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2069028.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3604..6306
                     /locus_tag="U4B03_002205"
     CDS             3604..6306
                     /locus_tag="U4B03_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2069029.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6458..6775
                     /locus_tag="U4B03_002206"
     CDS             6458..6775
                     /locus_tag="U4B03_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2069030.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6805..7125
                     /locus_tag="U4B03_002207"
     CDS             6805..7125
                     /locus_tag="U4B03_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2069031.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7233..8693
                     /locus_tag="U4B03_002208"
     CDS             7233..8693
                     /locus_tag="U4B03_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2069032.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8761..8982
                     /locus_tag="U4B03_002209"
     CDS             8761..8982
                     /locus_tag="U4B03_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069033.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9013..9195
                     /locus_tag="U4B03_002210"
     CDS             9013..9195
                     /locus_tag="U4B03_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEN2069034.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9195..9608
                     /locus_tag="U4B03_002211"
     CDS             9195..9608
                     /locus_tag="U4B03_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEN2069035.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9731..10912
                     /locus_tag="U4B03_002212"
     CDS             9731..10912
                     /locus_tag="U4B03_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2069036.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10983..11147)
                     /locus_tag="U4B03_002213"
     CDS             complement(10983..11147)
                     /locus_tag="U4B03_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069037.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11301..11374
                     /locus_tag="U4B03_002214"
     tRNA            11301..11374
                     /locus_tag="U4B03_002214"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11335..11337,aa:Arg,seq:cct)
     gene            complement(11443..11718)
                     /locus_tag="U4B03_002215"
     CDS             complement(11443..11718)
                     /locus_tag="U4B03_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2069038.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT         3946 a         2020 c         2513 g         3515 t
ORIGIN      
        1 ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc
       61 gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc
      121 tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt
      181 aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga
      241 gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac
      301 ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga
      361 gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa
      421 aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata
      481 catagtagga agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta
      541 ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg
      601 cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta
      661 ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga
      721 agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt
      781 tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag
      841 taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat
      901 ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc
      961 ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg
     1021 ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag
     1081 aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc
     1141 tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg
     1201 atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact
     1261 taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa
     1321 aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt
     1381 ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc
     1441 atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag
     1501 caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa
     1561 tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa
     1621 atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa
     1681 tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag
     1741 aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt
     1801 accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa
     1861 gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg
     1921 atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg
     1981 actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc
     2041 gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg
     2101 aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt
     2161 atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa
     2221 atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca
     2281 agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct
     2341 tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc
     2401 tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg
     2461 tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga
     2521 tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc
     2581 caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc
     2641 aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat
     2701 ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat
     2761 tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc
     2821 ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga
     2881 ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga
     2941 tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca
     3001 actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag
     3061 tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa
     3121 aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct
     3181 tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg
     3241 aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct
     3301 aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata
     3361 cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa
     3421 aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat
     3481 aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata
     3541 agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt
     3601 tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg
     3661 acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta
     3721 agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct
     3781 ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact
     3841 tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct
     3901 gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg
     3961 aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca
     4021 gatgcgccat ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt
     4081 ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt
     4141 ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg
     4201 gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa
     4261 agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa
     4321 agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct
     4381 tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat
     4441 cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat
     4501 gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt
     4561 ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt
     4621 caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg
     4681 gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa
     4741 gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc
     4801 gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat
     4861 ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat
     4921 gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt
     4981 tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg
     5041 agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg
     5101 gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt
     5161 ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag
     5221 cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca
     5281 agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg
     5341 ccactggtta tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat
     5401 caagagcatt tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc
     5461 agtgatttga tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc
     5521 atgattgtga tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat
     5581 caaaatatcc agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct
     5641 gaaactttac agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct
     5701 gccaaattat atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag
     5761 atgcagtttt tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat
     5821 gagatcaaag caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca
     5881 gcctatgacg tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag
     5941 atcccgacag ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt
     6001 gatgaactga tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt
     6061 atcgtggaac ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga
     6121 ttgatcaaac aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat
     6181 cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta
     6241 aaaattccag aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa
     6301 aaatgaacac actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa
     6361 gagctaaaaa gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt
     6421 tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt
     6481 agtttgttca gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga
     6541 agaaaaaggg atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt
     6601 agaatctaag aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca
     6661 atttgaacaa aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta
     6721 tggcatgatg aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa
     6781 gtatgcagtt gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg
     6841 ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa
     6901 atcggtgatt ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa
     6961 gcgcatcatg cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg
     7021 acattattga ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta
     7081 gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt
     7141 tgaacggaag aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct
     7201 ttatataaaa aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga
     7261 aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg
     7321 atgcttttat cggcatactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg
     7381 caattattcg tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag
     7441 acaaaggaat ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg
     7501 gtacactagc aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag
     7561 cttatggagt aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta
     7621 tttcctttgg tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag
     7681 atgcaatcaa tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat
     7741 tatggggttg gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca
     7801 tgggagctat ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa
     7861 tgcctgattc agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga
     7921 tcgctttata cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac
     7981 tgatcattga tttggtgcaa aaatatattg cagaaccatt cctaggatta tctcaagggt
     8041 tgggagcagt attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg
     8101 gatcaaatgt attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata
     8161 ttgatatttt ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa
     8221 tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg
     8281 gcggatcagg cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag
     8341 attatctaac agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag
     8401 tgatgtttgg tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc
     8461 cagtagtagc aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat
     8521 cgcagcaagt cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg
     8581 attggcgggc accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac
     8641 catttgtcat ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac
     8701 gtgaggagcg ggacgcttta agtttcgttc ctcataatga attttcgagg aggaactgaa
     8761 atgattacaa ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac
     8821 aatgaagaag gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa
     8881 acaagtgcag aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag
     8941 ccttggggaa cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat
     9001 aggatggttg agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg
     9061 ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt
     9121 tttctaacag gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag
     9181 gagaaaaata actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg
     9241 ataaagttat ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta
     9301 gaaaacagct gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca
     9361 taaaaatcga agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga
     9421 atgaattcgt cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt
     9481 acacagaaaa aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa
     9541 caatcttgat gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa
     9601 cgtattaaat caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag
     9661 gaatttttca taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag
     9721 gggcagcaaa atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt
     9781 tgatttagcc acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg
     9841 tacacttgcg gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag
     9901 tcaagtcatg gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg
     9961 cattggctat gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat
    10021 ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt
    10081 ctcctatccc ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca
    10141 actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga
    10201 ttttgaagat gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg
    10261 agctggtctg aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta
    10321 ccagtttgct ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct
    10381 tgcaacgtct tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag
    10441 ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg
    10501 ctacggcggt aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac
    10561 accttatatg atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa
    10621 aaagagacaa gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga
    10681 aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac
    10741 aaaactccca atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg
    10801 taaagtggaa gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac
    10861 gttaagctca atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac
    10921 gggtgatgac aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa
    10981 tgttaaagta aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg
    11041 ttttcttttt tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat
    11101 gaatctaagc gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc
    11161 cctacattat ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt
    11221 gcctaactta taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct
    11281 acaattgatt tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag
    11341 cgataggtcg ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgcg
    11401 gtacaggggt ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata
    11461 taaactatcg aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat
    11521 atgaagataa gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct
    11581 aagtatattt actccttgat aaagtaaaat agatgcatgc gtatgtctta gtccatcaat
    11641 aaacagtcga agagaaaata gggaaaaatt gattactaaa gaaaaactta ctaggtcgac
    11701 ggtgtagttg attttcatac ttcaatagat tcgaattaaa cgatttcgat tggcttatac
    11761 cagtcctatc caacgaaaga tatatcgttc ttaactctag cggtataact tgaagcttta
    11821 cgtaaagcca atgggaatga actggagatg actttttatt atttttagta atagctgatg
    11881 ggttaatact tacaactttc cttttcaaag taccatataa aaaaacggtg gggttgttta
    11941 gcgaaataaa ggatctaagc caatagctta gatcctttat tctatgcttt atat
//
LOCUS       DASJSZ010000080        11908 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.084, whole genome shotgun sequence.
ACCESSION   DASJSZ010000080 DASJSZ010000000
VERSION     DASJSZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.084"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..465
                     /locus_tag="U4B03_002216"
     CDS             <1..465
                     /locus_tag="U4B03_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN2069039.1"
                     /translation="WKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAF
                     LKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1080..2120)
                     /locus_tag="U4B03_002217"
     CDS             complement(1080..2120)
                     /locus_tag="U4B03_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN2069040.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2469..2795)
                     /locus_tag="U4B03_002218"
     CDS             complement(2469..2795)
                     /locus_tag="U4B03_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069041.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2782..3585)
                     /locus_tag="U4B03_002219"
     CDS             complement(2782..3585)
                     /locus_tag="U4B03_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2069042.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3938..4228)
                     /locus_tag="U4B03_002220"
     CDS             complement(3938..4228)
                     /locus_tag="U4B03_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN2069043.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4364..4543)
                     /locus_tag="U4B03_002221"
     CDS             complement(4364..4543)
                     /locus_tag="U4B03_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069044.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4540..4890)
                     /locus_tag="U4B03_002222"
     CDS             complement(4540..4890)
                     /locus_tag="U4B03_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069045.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4883..6208)
                     /locus_tag="U4B03_002223"
     CDS             complement(4883..6208)
                     /locus_tag="U4B03_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN2069046.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6658..7272
                     /locus_tag="U4B03_002224"
     CDS             6658..7272
                     /locus_tag="U4B03_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2069047.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7437..7679)
                     /locus_tag="U4B03_002225"
                     /pseudo
     CDS             complement(7437..7679)
                     /locus_tag="U4B03_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7883..9202)
                     /locus_tag="U4B03_002226"
     CDS             complement(7883..9202)
                     /locus_tag="U4B03_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HEN2069048.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9357..9944)
                     /locus_tag="U4B03_002227"
                     /pseudo
     CDS             complement(9357..9944)
                     /locus_tag="U4B03_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10582..10839)
                     /locus_tag="U4B03_002228"
     CDS             complement(10582..10839)
                     /locus_tag="U4B03_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN2069049.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10832..11101)
                     /locus_tag="U4B03_002229"
     CDS             complement(10832..11101)
                     /locus_tag="U4B03_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2069050.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11232..11411)
                     /locus_tag="U4B03_002230"
     CDS             complement(11232..11411)
                     /locus_tag="U4B03_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069051.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT         3675 a         2151 c         1826 g         4256 t
ORIGIN      
        1 tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc
       61 atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca
      121 gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca
      181 gataaagcac catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa
      241 gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca
      301 gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc
      361 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
      421 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta
      481 agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt
      541 aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga
      601 gattaccagt ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca
      661 atttatcgtc caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa
      721 atttaaaaca actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa
      781 aagaagtaat atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat
      841 tattataact caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa
      901 taactaaaat atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac
      961 ttttaatcta aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa
     1021 aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac
     1081 tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg
     1141 tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt
     1201 agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc
     1261 atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact
     1321 gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga
     1381 gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat
     1441 gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt
     1501 aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat
     1561 agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta
     1621 tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg
     1681 attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct
     1741 tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg
     1801 tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt
     1861 ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg
     1921 aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga ataatcaaat
     1981 cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg
     2041 ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg
     2101 taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac
     2161 tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa
     2221 gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa
     2281 ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata
     2341 ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca
     2401 gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg
     2461 aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct
     2521 tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca
     2581 gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct
     2641 gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat
     2701 tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta
     2761 cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata
     2821 ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag
     2881 atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa
     2941 aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat
     3001 gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt
     3061 atattctttt accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata
     3121 cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat
     3181 tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag
     3241 tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag
     3301 ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc
     3361 agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc
     3421 tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta
     3481 aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt
     3541 ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata
     3601 atataattat atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat
     3661 tgtgtgatta ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt
     3721 tattcgaaat taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa
     3781 tatgttttgc gagcagcaac tagaataatt taaacattac taaactgtct atattgttac
     3841 taataattcc cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat
     3901 attaagatat cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt
     3961 tatcatgttt aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa
     4021 catcagacat gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg
     4081 attttattct acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata
     4141 actcacaaaa ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt
     4201 gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg
     4261 tttgcaataa tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa
     4321 aaaaggattc atagagataa ctctatagaa tccgtaaaat accttatata tataacggga
     4381 agatataaaa cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct
     4441 caacaccaac ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat
     4501 caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc gactccattt
     4561 attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac
     4621 gtagatcccc agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc
     4681 ttcagcgttt cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt
     4741 caatacttcc gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg
     4801 ttgttcattg tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt
     4861 taaacgtcca cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac
     4921 caccaacaag tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta
     4981 caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt
     5041 cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa
     5101 gcttgctata ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca
     5161 agaaaagaca ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt
     5221 gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg
     5281 ttttacagtt atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca
     5341 gctcgatttc atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt
     5401 tttcctttgt cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga
     5461 agtacagctg taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag
     5521 caagatctct aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa
     5581 ttccccaaaa gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg
     5641 tccattccgc aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca
     5701 aaaggggatt atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct
     5761 cctggatcat ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa
     5821 gattcagtga tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt
     5881 catcagccac atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt
     5941 tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc
     6001 tactcacatt tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc
     6061 ctgaaccagt attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat
     6121 ttctttctat acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat
     6181 ttactggctc ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa
     6241 catatgttcg atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat
     6301 gctatcaccc tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat
     6361 taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt
     6421 acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat
     6481 ttgagaataa gttatcagta gacacttaca aaaaattctc atactatttc ctccatcatt
     6541 agtttttgtc tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg
     6601 cttcctcgtg tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg
     6661 agagttgcat acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa
     6721 atgaaaaaat tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact
     6781 catcgacctg ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg
     6841 gaagctattg atcgacttgg aagaaattat gacgagatca tcgcttcagt caattacttg
     6901 aagaagaaaa atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc
     6961 aatcctctcc tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct
     7021 gaacaagaac ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca
     7081 aacggcgtct acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt
     7141 cgccttgttt acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt
     7201 gctaaagact acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc
     7261 aatgacaaat agttgatcat gctgctttta ttatcttctc atctttaatt tcctacatat
     7321 aattactatc tataaaatta agtcttactc agaacagata aagcataaaa ttccaacaaa
     7381 ccatttttta cttaactaaa taaagagtct gttaatataa tcttcactta tttacgttaa
     7441 tcccctactg tatttgttgg ttccttatct atattttcta caaaatcctt attatcttgt
     7501 gatttagctt cgtataattc tttgtatgtc gacacattgg ttatactaat tcctatagct
     7561 agttcttgag actctacttt tctctctagc tcctttgaca aagtgatttg agatttattt
     7621 ggttccgttg gagtcaattc tatagattct tcataaaaat ctctatacat aaagctatcc
     7681 ctgaataatt catagcttta attctttggc acattttatt gaaatatgta tcactaatat
     7741 ccctatcaac tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga
     7801 ggaataaatt ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta
     7861 aaaaaatcgt tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga
     7921 ccctgtagaa ttcattctga tacacatgat aactagataa tttgagatag actcgtctac
     7981 ccgtttggac taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca
     8041 ttcccttttg gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta
     8101 tcaagctgag catcattcgg acatgattct ccaaaaagcg aggactgtct gttttgtcaa
     8161 agtaaaagcc agctttcgct tctttaatga agttctccat tgtgccacgt ttggcataga
     8221 tagaaaagat ggtatcagga gaaacatttt ctgatagatt cgtcacgata aattcatgtc
     8281 gaaagagtag ttcgcccgct tcacgtgttg agcgtataca cacgcgacga ctttgtgacc
     8341 aggattgtgc ttgataagtg gtcgagaagt actgaacttc tcgttcttcc cacttttgat
     8401 tatcgccata aagtactgat ttctcagcta tttgacctag tctacgatta ttcttcagtc
     8461 gaataacata atgactcttt tttgattcac acgaatcata cacaccaggt gtagcgaacc
     8521 cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga gtgattatag tgctctaata
     8581 aaggtgtgag aaactccttt acgccttttg atgtatattg atttcctgaa cgtagttcag
     8641 cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa ccataggttt
     8701 ggtagtgtgt attataattc gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat
     8761 ctaaatcaat gattaattcc gtatcattac ggatgaggcg tgctttatcg ataagttctt
     8821 ggttcaaagc ttgaagttca tggatattct cctcagatag tcgatctaaa aatcgggaaa
     8881 gtgaagattg agaagcgagt tctttcctat ctaaaacagc tttaaacaca ggatcttgtc
     8941 tcaggagatt agctgcagaa tcagctgagt aaccagcaat taattgcata atgaactgct
     9001 caagtatgga taagttgtca tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt
     9061 gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg acgagacccg
     9121 aatcactcga taattgacca cctgtatgag aaatagtgat atttgaattg aattttactt
     9181 ggtttttgtg taagctagtc atgaaaagaa ctcctttctt ttggttggtt tagtcacttt
     9241 aaccatagca gaaaggggtt cttttttcat cacttaacgg gtgaagatga aaaagtaatt
     9301 gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa agtatgaatt attcaggcta
     9361 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     9421 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     9481 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     9541 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     9601 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     9661 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     9721 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     9781 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     9841 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     9901 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     9961 aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc aataaattct
    10021 atttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
    10081 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
    10141 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
    10201 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
    10261 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
    10321 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
    10381 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
    10441 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
    10501 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
    10561 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
    10621 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
    10681 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
    10741 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
    10801 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
    10861 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
    10921 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
    10981 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
    11041 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
    11101 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
    11161 aaatgattaa tattctaact tcttataatt aaaagtgcta tcacttttag tttttaggat
    11221 aaaaagtgac gtcacttttt gagtgctggt acctcaaatc ctgctttttc ttgggcaatc
    11281 aataaatcta acgcaataca cataatcctg gattcacttt tgccgtactt ctgctttagt
    11341 tgctgcaatt ctttttgtct cttttcatcc aacgtaacgg gtaaccgttt atttcctatt
    11401 gcaattggca ttagtactct ctcctttcat cgaaataacc ctatgaaaaa tatggctgca
    11461 atcatcttga atagtagact cacaagggct aatacaaagc gccaaaagat caaacaaaat
    11521 accagtattg ccaaaaatac tacgaagttt aaatgcacag ctgcagcatg gcgcataagt
    11581 atttcgccaa caaataggac taacaataga ggaatgattt tattcatatg gatctccttc
    11641 tttctcttag gttatcgggt gttgcccgct tatttcgcac cacctaagcg gcgggggcgg
    11701 ttggagcgat tggtgcgaaa taagaaccat tcccttagtt actaggtttt ttgtccttat
    11761 ttcacacatt ctcacttttc ggtataaatg attattgcct ttttttcctt tcaaatcgtt
    11821 gatatggcat tcgtttacgg tcaagttcat tttggatttc ttctgtatcg tcgtaagtat
    11881 aggtgtctaa aaaatcagtt aaagtcgt
//
LOCUS       DASJSZ010000081        11826 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.085, whole genome shotgun sequence.
ACCESSION   DASJSZ010000081 DASJSZ010000000
VERSION     DASJSZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.085"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            93..377
                     /locus_tag="U4B03_002231"
     CDS             93..377
                     /locus_tag="U4B03_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HEN2069052.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(665..1339)
                     /locus_tag="U4B03_002232"
     CDS             complement(665..1339)
                     /locus_tag="U4B03_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEN2069053.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(1329..2054)
                     /locus_tag="U4B03_002233"
     CDS             complement(1329..2054)
                     /locus_tag="U4B03_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEN2069054.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            2581..3783
                     /locus_tag="U4B03_002234"
     CDS             2581..3783
                     /locus_tag="U4B03_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN2069055.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            complement(3960..5228)
                     /locus_tag="U4B03_002235"
     CDS             complement(3960..5228)
                     /locus_tag="U4B03_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEN2069056.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(5389..6036)
                     /locus_tag="U4B03_002236"
     CDS             complement(5389..6036)
                     /locus_tag="U4B03_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEN2069057.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            complement(6301..6804)
                     /locus_tag="U4B03_002237"
     CDS             complement(6301..6804)
                     /locus_tag="U4B03_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2069058.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            complement(6928..7692)
                     /locus_tag="U4B03_002238"
     CDS             complement(6928..7692)
                     /locus_tag="U4B03_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEN2069059.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            7800..8075
                     /locus_tag="U4B03_002239"
     CDS             7800..8075
                     /locus_tag="U4B03_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEN2069060.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            complement(8423..9574)
                     /locus_tag="U4B03_002240"
     CDS             complement(8423..9574)
                     /locus_tag="U4B03_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2069061.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(9594..10316)
                     /locus_tag="U4B03_002241"
     CDS             complement(9594..10316)
                     /locus_tag="U4B03_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2069062.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            complement(10309..11760)
                     /locus_tag="U4B03_002242"
     CDS             complement(10309..11760)
                     /locus_tag="U4B03_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN2069063.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
BASE COUNT         3484 a         2455 c         2010 g         3877 t
ORIGIN      
        1 aattcacgaa aattgctttt caaatttttg tgaattttgg cttatttccg aaggagttgc
       61 ttctgctccc accgtttatt cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt
      121 cccactctca gaaagtaaaa ccgatcttct ggctttctat ttgggacgga tttttcaaag
      181 tatcctttca tttcccagaa aaaacgaggg agaaattagt cacactctca ttgagtgaag
      241 gaaccaaaga gaagataaaa actcttcaag caaatgggaa taaaaggaaa catctttcgg
      301 ttatctttga tgttgatcat ctggaacaat tggctgatat ctatcaacta gcggaattta
      361 agaaacagaa tggatagtct tccttagatt cccagctatc agataccagc caacttaatg
      421 caattttttt atggccatcg catctttgac tgttgctaac tcgtttaaga tcaacgaaac
      481 agccttgcaa ggaaattgct cgagataaaa agggaggttg atctttatcg ctgtactctc
      541 acgaaagctt gctctcagtt ttaccagtag ttgctgaaga caaaaaagaa agtactttat
      601 tgaagtgtcc ccgctaaact ttggaactgg ggtcttactt cattgttaag tactttcttg
      661 gttatcacaa taatttattc gatttatcca tacctacagt gatcgaaatg acttctggat
      721 gatccatgat cgttcgaacc aattcttttt caagcatact cgtttttact tttactatat
      781 agtgtaagga acaagtttct ttattgattt ttactttttt taaattgcag ttttctgtat
      841 attctgcaat tttttcttca ataatactcg aaagaaacaa ggatttagaa acttttagct
      901 tgatcgtttt ttcagtatta aaaactgtat gtttattttt gattggaagc gtgaagatga
      961 tgacttggat agcagcaact atactagaca cgaatagtgc atagacaata tatcccgtcc
     1021 ctgtggctaa accaatcgac gttgcccaaa aaatactgaa aatatctctt gaattccctt
     1081 gagcagaccg aaatcgcact aagttaaatg cgcctagaac agcgatcccc gtgcctaagt
     1141 taccgttgat caacaaaatg acggtatgta ctaaagtagg taacccaatc aaggcaaaca
     1201 tgaagttttt tgtacagttg ttttgataca tatgggtgta agcaatcaga atgcctagca
     1261 aaatcgacac caccatacat aaaaagaaat catctagtgt gagttgagaa ggctcatttt
     1321 ttaagacact attgaacata gagcgggtcc tcttggatat atcttttata gacttgttta
     1381 tatttagaaa attttatctc ttttttaccc atcctatcca atcctacctg taaccattca
     1441 ggtatttctc ctgtcgtttt tacttccatc agcacatcga tttctggcgc taccagattg
     1501 tcttctgtac agtgttccca ggtcgttaag ttcattagac ggtaatggat attttgatca
     1561 aaagtgattc gcaaatcttc tgaatctcta tttacaaatg cttctcttaa ataatagata
     1621 tatactttgg gctccaatgc caaccttctg aacaaccact gtatttcatt aaaaatatac
     1681 tcttcttcta aattactgaa tgtggtttct gccaattcga tattttccaa catatcatcg
     1741 agctcatgat gcgctaattt tattctacgt ttatctgtca ttccttgaaa ctttcttttt
     1801 atctctaagt agataggcga tatgtcctga ggatctccat attttctgat tctaaatttt
     1861 tctcgatagg acttttctgt agtgatttct tttaaaaagt gatgatcgtt tgtatcaaaa
     1921 tacaaggaag aaataaaatg atcactatat ttatcccgaa ctacctgttt tggaaatagt
     1981 gttttcaaat acttagcttg gtcataagtt aatgcaaatt ttctttcaat acgtttaaat
     2041 ctttttttgt tcatatacta tctcctataa aatactttat ctcaaaatag aattactttc
     2101 gattatattt gtgacagtga atgtatagaa aagcgagtgg ataaaaactg atttttcatt
     2161 ctggcctact tgttccacgt tttactatgt atacttcttg tctatcttgt tttttccatc
     2221 aaatcatccg gttcattcct tccacagatt ttgttgccgt taacatttta ttatgcaact
     2281 ggtttattgc ttgaccgata ttttccaata aaaaaaaata tcgttttcgc attcattttc
     2341 aatgttacag ttatttgtat ctacaaaagt cttcatcatg atcgaggtct aaaaaatctg
     2401 ttaatttgaa gatttatgtt ttcgataaaa gcttttttca acataattcg ttataataga
     2461 aacgtcgtga aaaaactgat tgactattta gtaactaact tttctatatt cgatatattt
     2521 actactaaat gatgctatct tttttaagaa aaaatttaca aatcgaaagg agttcattca
     2581 atgaatctta ctagagacct gatattttta gttatctata ttttaggaat gtcaggcatc
     2641 ctctattggc gcaagcgcca aagcgatccc gctctattct tttattgtgg gacgtggact
     2701 tttatgatga tggtcactta cttcgtcgtt ttagaagttt tttatcatac attttatttt
     2761 ttgattccat tgctattttt cagtattttt gcatttttct attttaagga aaaacgtagg
     2821 ttaatcaatg gcgtactttt taatacgttt ttgattgtct ttggtggtta tctagggtgg
     2881 ctactaatag agacgcaaaa tatctttatc ggtgggttat tggctttgat cgggatacca
     2941 atattaattc ttttggcaat cggtatctac attttgatca tttttttatt ttggaatggt
     3001 gttgtagtca tgaaaaaaga atcccgttct ttggccaacc ttttgactct cttgttagga
     3061 gtcgctttga ctatcttttt gatattggac tatacattag tccattggct cccacaatgg
     3121 gcaaatgctc tatttgctgt cattccgttt atttttgctt atctactcat cgttttcttt
     3181 aattttatga ctgtttcaat tttgtatcag ttcaaccgtc caaggtataa ccaagattat
     3241 atcgttgttc tcggcgctgg cttgattgat ggtgaacgcg tatctcccct acttgctaaa
     3301 aggatcaata aggcgatcgc attttattgg gcacaaagta aagcaacttt gaatccgcca
     3361 gtcttgctca tgtcaggagg aaaaggaacc gatgaaaaag tagctgaagc cattgcaatg
     3421 aaacaatatg ctttagaaaa agggattccc gagcgtgata ttttagtaga aacgaattcc
     3481 acaacgacgc tagaaaatat gctgttttca aaagaaataa tggatcagca aatgaatggg
     3541 ccttatcgcg cgatttttac ttcaaataat taccatatct ttagagctgg actatatgcg
     3601 cgacaagcga aactcaatgc aaacggaatc ggtgcaaaaa ctgcactata ctacttaccg
     3661 aatgctatct taagagaatt tattgccatc cttgtcttac acaaaaaacg ccatcttatc
     3721 atctgtggct taatgattgg ctttgcagta ttttctgctc ttattctcaa atttgctcaa
     3781 taaaaaagag cgtgggacaa aactaaaaat cagttttgcc acagactcta actccgaata
     3841 aacggtggga gcagaagcaa ctccttcgga aataagcgaa attcacaaaa atttaaaaag
     3901 caattttcgt gaatttcttc ttatttctcg gagttaaaca cttctgtccc aacctctttt
     3961 tatttctctt tttgtcgtga gattcttcca aggatcggta ggaacaagat tccttttttc
     4021 acttcaactt tttgcgcaga atagattcca ctatttcctg aacacataaa gctaatcaaa
     4081 cagatcataa agaagtagct ggctgcttga cctccaaaaa gttcgatccc cataacaaag
     4141 caagcaatcg gtgtattcgt tgctccagag aacaccccaa taaaccctaa tcctgctaaa
     4201 aaaggtactg atacatgaag aatgggtgct aatgtcgcac ctaaagttgc cccaatctca
     4261 aacaaagggg tcacttcgcc tccttggtat cccgcgccaa gtgatagaac ggtaaagcca
     4321 agtttcccaa gaaaatcata aggatgagca cttccattaa atgcatcttg taataaaggt
     4381 aagcttaatc cgagatatct ttgcgtctgt aaaatcacta ctacgagtac cacgactgct
     4441 cctccaataa agttgcgtaa aactggatgg gggatccgct ttgcatacca agcttttaac
     4501 cagacgatcg aacggctaaa gagccagcca atgataccaa atgcaatgcc cgcaagcaac
     4561 aatttgaaga aaagtgaagg gtcccatgca ggaatcgccc ccatttgata atgggtatgc
     4621 ttgacattga aactttccgt tacaaagttc gcaaaaagtg ctgcaaaaaa gcttggaaac
     4681 agtgcttcgg ttctcacttt tccgattgcc aatacttcta atccaaacaa tgtcccagcc
     4741 aagggtgtcc caaatactga gctaaaacct gcgctgatcc cactgatgac aagaatctca
     4801 cgctcgattt tgttcaattt tagccaacga gcaaaattct cagcaagtgc cccgcccatt
     4861 tggaccgctg taccctcccg accaacagaa ccaccaaaaa gatgcgtggt aatggtgcca
     4921 aataaggtca gtgggatcag ccgtaatgga atcttttcat ttcctccatt cccttgatcg
     4981 atcactaaat tgtttccccg ggaagaattg cccccatacg tcgtatatag ataagcaaaa
     5041 agtcccccac ttatcggcaa aagaaaaagt aaccaaggat gctccagccg aatagctgtc
     5101 acatcagtca aactagttag aaagaatgcg gataaacttc ccataaaaat acctaaaatt
     5161 gctgtgatca ataaccattt gatcatatag atgatcacct tcaatggatc aggtaatcga
     5221 ttttccatct catttcctcc tcaaaaaaac gcctactaaa aaaaggggta gccccgtttc
     5281 tttagtaggc gtcattagtt atcttacgat caacgtttaa aggcgaacac catcacctat
     5341 tcacttcacc aaataagata gcatattttt cagaaaaata caatgaaatc accgctcacg
     5401 tttcactcgt tctgtgaatt cgtaaaaagg agctaattgt accaactgct gattacattg
     5461 gtcactatca tgacaaatat aatttccttt tttcgtgtat gttccatctg aaccagtttt
     5521 tgtggtagaa agaaagagtg aaacatttga agttctttga caaatcgaac aaattccttt
     5581 gttgattgta ggagataatg ttccactgat cccttttagt ttttcttctt cataatagat
     5641 cataaatttt ttttggctac ctggatcatt ccaaccaata taagtatgtt cccttaaatc
     5701 cagttgattc caatctggtt gttttaactt tttcactttt cggaataatt tatcgatctg
     5761 cggttttgtc ggttgtatga atggaagaac gatcactttc aattcctcta atagtcgttc
     5821 tgctttttct ttttttagag tgacgtctag taccttttcc ataaattcat ttacagctgt
     5881 attaggatga ccaagtacct cgtggatctt ttccgcagct aatgcttgga ctgtttggac
     5941 tgtctttgga tcattcactg aactataggc gcttaacagc tgctcgactt ctcgcttgat
     6001 aaaataatat tgatatggtt tgattgtttt ttccatgact ttgagtcctt tcttctttca
     6061 aaatcggcta ctttgaagaa aaaagtgatc tttctatcaa agtgccttta gataaagccc
     6121 tcaaactctt ttaaataaga tacacatcgc atcttatcat cacccccttt atttttatgt
     6181 tggttatgta ctttttcatc tcaacaaagt aaacactaaa aagaccaatt agtcaatgtc
     6241 taaacaaaaa acccgaggat ttttttcaaa atactcgggt caaatactta gttaactacc
     6301 ttaaatcgat tgttctttac cgaaatatcg ttgccatttt tgtttcattg aagctgtcat
     6361 tggttgagct gcttctttga ctgcacaata tttatccaca aaagtaacga tataaccttc
     6421 tttatattta ggaggggcaa tcgttgctcc ccacatatgt ttcaaaataa tatcacgctc
     6481 tagatctgaa agctccgtga ttttttcagc atttttcaca gcgatccttg ggtgaacata
     6541 cgcatgtgta ccttcatcga acttcgtcgt acgccaatcg tagtaaaata aatcatgtaa
     6601 aaggcctgct cgagcagtcg ctcttgcatc accgccccat tttttcgcta atttgtagct
     6661 atagtaagac acactgattg aatgctctaa tcgtgtcgag tggacatgtt gcgtgtaatt
     6721 tgctaatttc tttactgcat cagtttctaa tagatcattt atataagaca tatattcttc
     6781 gtcttcacgc catttttctg tcataattat cgatctgcca cctttttgaa ttccatttat
     6841 ctttgaaatt gaacttagta taccactagt gttcgtaata ttctagtgag gttttcgcaa
     6901 aatgcagaaa gcttaatagt tccttaacta tacggagaag tgataaagca tgatgcccgc
     6961 tgcaactcct acatttaaag actccgcatt tccttgaatc ggtatataca catttttatc
     7021 cgttagcgcc agcacttctg gacgtacgcc ttgaccttca ttccccatca cgatcgcata
     7081 attttctgtt gtctggatat cattcaggga cactgcttct tcattcagtt ctgttccata
     7141 ggttataaat ccttgctctt taaggatagg caatagtttt gttagatcct cttggatgat
     7201 cggtaaatga tagttactcc cctgcatgct tcttaatact ttggtactgt agacatccgc
     7261 cgttccctta cctagtacaa cacaagatag accaaaagca tcggctgtgc ggatcattgt
     7321 ccctacattg ccgggatctt gtacgttgtc gagcaacaac caaccacctt caaaattcgg
     7381 tatctctctt ttttcagctt ccaccactgc catgatccct tgcggagttg gtagatctga
     7441 aacagttgcc atcacttctt ctgatactag tgtaacagaa agcgcctgat tttcttcaag
     7501 ccaactagcc cactcagtca gaccgcgttg cgtaataaaa agttcgttga ccttcgcatt
     7561 tgaggcaaca gcctcctcga tcaggtgaaa cccttcaagc agatattccc ctttttcttc
     7621 acgatatttt ttcttatgta atttttttaa ttctttgatt cttggatttt ttgctgattg
     7681 gatctctttc acatttttca cctcaactga ttataccatg gattagatca ttgcacttgt
     7741 tcagtagcaa aaaagtcggt atcattaatt taaaaataaa agaaagaacg gtgaataaaa
     7801 tgaacaaaat caagatgaac gtgcaaggac gtgtacaaag cgttggtttt cgctatatga
     7861 caaaaatcgt agcggatcaa ttagggatca ccggaacagt gaaaaatgaa gatgacggct
     7921 ctgtcacgat cgaagctgcg ggcgacgatg aggcaatcaa aaaattcgtg gaagaagtca
     7981 aacgatcacc aagccccagc ggacgtgtcc aatatgtaga ccttcaggaa aatccgatga
     8041 tggaggacag aaaaaagttt gttgtaatag gctgaccttc tagaaatgaa taggaaatgt
     8101 cagaaaaatc ggactgtctg tatgtagatc gaaaaagttt gcccctctct ccatcctaga
     8161 tcgacaaaaa aacattctta ttagcgagtt tagtaaccag tgataggcat acgtaaaagc
     8221 gggaggactt caaaaatttt ggttcaataa aatggagtct ataatatttt atggtagtgg
     8281 aaaatcgact ttgattttcc actaccactt tttcattgtc aaatcctgac gctgcccagt
     8341 caacaagcca actctcgaaa ggatcatttt ccctctttgt tctaatggat acccatagct
     8401 gttttcaaat atacagtttt atctaaaaat ttagttttgc taaacgatca aaggcttcac
     8461 ctaagatttc caatggctga gtcgctgcta gtcgaatata gttgtccccg atcgtttccc
     8521 caaacgcttt tccagggaca aaaagtacgc cgcttttttc taaaacctct tccacaaacg
     8581 ttactgaatc gagcccactc ttactgatat taataaaagc atacatgctt ccagaaacag
     8641 aagataaaga taagtaaggg atttctgcca ctcttttctc aatataagct aatcgttctt
     8701 tgaatacttc aacaactggc ggtacaagct gttcatagtg ctttaaggcg tagattgcac
     8761 ctctttgaga agcagtcggt gcggaataag tgatgttttc attgatcaac ccagcaattt
     8821 gattgatgta tgctggtgcg atcatgtacc ctaagcgcca gccagtcatc gcaaacccct
     8881 tcgatacacc actgacagtg atcgtattat cgggcgcaaa tcgcgccatc ggtacgaact
     8941 tctctttaaa ggcaaagtct tcataaatct catcggaaat gatatagagt tcattttcga
     9001 ttgcgacttc agcgattttt ttcaacgtgc tttcagaaaa aacagctccc gtaggattat
     9061 tcggcgaatt gatgacgatc acctttgtac gatccgtaat cgctgctttt aaatcttcca
     9121 catccaactg gaagcccttt tcttcatagg taggaacaaa aacaggcttg cctccagata
     9181 aaatgacttg ttcccgatat ggtgaaaaat aaggttcatg gatgatgacc tcatcctctg
     9241 gatctagtaa tgcctgtaaa actagataca tcccgtgcat tgctccgact gtagctcgaa
     9301 cttggttgca ttcaacgctc aatccatatt tcttttgata aaaatctacg accgttgaca
     9361 agaattcttt gctgccatca ggcgcagtat aattagtagc accatttttt acatctgtat
     9421 aagctgcttc aataatcgtc ggatcagtga tcaagtcagg atcaccaatc gataaatcga
     9481 taatatcctc tttttccttt gccaaaatag caatattcat caacagattt tctgctggtt
     9541 gctgaaaccg gcgtgcaatc ttacttttgt ccatcttact tcctccatct ttgttacaac
     9601 actttgttca agaaatcctg tgttcgacta ttttgcggat tttcaaaaat ctctttcggt
     9661 gttccctctt cttgaatgat gccgtcagcc ataaaaatca cacgatcggc aacttcttta
     9721 gcaaaaccca tttcatgagt gaccacgacc atcgtcatcc cttttttagc taaatcctgc
     9781 atgactgcca acacttcacc gaccatttca ggatcaagtg cagatgtagg ttcatcgaaa
     9841 agcatcacat caggattcat tgctaatgct cgagcaattg ctacccgttg ttgctgtccc
     9901 ccagataaac tggtaggata actgtccact ttttccttta gaccaacttg gttcaataat
     9961 gatagagcat gttcttttgc ttttttaggg tcttcttttt tgatttttac aggtgtgatg
    10021 gtcaaatttt cgagtacatt catatgggga aacaaattaa aactttgaaa taccattccc
    10081 attttttgac gaatttgatt gatatcgctg tcaggggctg taatgttttg tcccttaaaa
    10141 aagatttcac cagaagttgg ttcttccagc agattcatac aacgtagaat tgtacttttc
    10201 ccacttcctg aagggccaat gatcaccact acttctcctt ctttcacaga aagatcaatt
    10261 gattttaaga cttcattctc gccaaaactt tttgacagtt tttttacatt aagcattcag
    10321 acccattctc ctttcaacta aagctaatat acgggaaata ctgaaggtca acacgaaata
    10381 aatcaaagag accaacacat atggtaggaa tggtttgaag ctagcacctt gtaccacgcc
    10441 cgtttgaaag atcaattccg ataccccaat gaccgaaaca actgaggact ctttgatgat
    10501 cgtgacaaac tcatttccta gagctggaag aatatttttg attgcttgtg ggaagatgat
    10561 gaaacgcatg gcttctttct ggtccattcc taaagaacgt gcagcttcca tctgtccttt
    10621 gttgaccgca ttgatcccgg cacggacaat ttcggctaca taagcagcac tattcaacga
    10681 gacagcaata caacctgcta ccagtttcga tagatccaat cccagtactc ctgttccgaa
    10741 gtaaacgata aagatttgaa ctaagagagg tgttccccgt acgtattcga tataaataat
    10801 ggctaatgcc tttaaaaatt tattcttacc caatttcatc agtgcaagca aactaccaaa
    10861 gaccattcca aacaatacac ccacaaaagc taataaaata gtgtattttg ttccaatcaa
    10921 ataatacgga ccgtattttt gaagaaagga ctgatcatct tggaacatca attgattcgc
    10981 ttgtttttga taatctttca gcaagccgtc gtcattgatc gttttgattg acgcattgac
    11041 atgatttttc agttctggtg tattttttga catcgcaata gctgtggttt tttcgccttg
    11101 cgcaaatttt actggagtca aagctacttt cttattctgt tgggcatatg cttctgcaac
    11161 tggttgttcc agcacaacac cttcaacttt ttcattcagc aaattattga tcagatcagg
    11221 aactttttgc agggaggtca actctgcacc agtcagttca tttttcacta actcctcttg
    11281 tgtggtttgc ttttgcgccc caacggctaa gccagaaaag tcggctgtcg tcttaaattt
    11341 atttttgtcc tctttacgaa cgagtacttt ttgctgtacc acgaaatatg ggtcagaaaa
    11401 gtcaacttct tgtaaacgtt cttcagtcac cgccatacca gatataacta aatcgatctt
    11461 gccagttttc aacgcaccta ataatgcatc aaaaccaagt tcttctactt ttagtttcac
    11521 acccatatcc tttgcgatct tttcggcaat ggatacatca aaaccaacga ttttatcctt
    11581 accatccaca ttcgcatgga attcataggg cgcatagtct gcagataggc caacgactaa
    11641 ttctccactt tgttttatcc gctcgtaaat ttcatctttt tctgcacctt gtacttttac
    11701 tggtaaaaaa accagtagaa acatcagcgc cactaataat atgtttattc cttttttcat
    11761 ccatttggcc acctttgaat aataatgtaa tcagaaaagc tataaaagaa taaatattca
    11821 taaata
//
LOCUS       DASJSZ010000082        11783 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.086, whole genome shotgun sequence.
ACCESSION   DASJSZ010000082 DASJSZ010000000
VERSION     DASJSZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.086"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            510..1073
                     /locus_tag="U4B03_002243"
     CDS             510..1073
                     /locus_tag="U4B03_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069064.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1134..1499)
                     /locus_tag="U4B03_002244"
     CDS             complement(1134..1499)
                     /locus_tag="U4B03_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069065.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1575..1820)
                     /locus_tag="U4B03_002245"
     CDS             complement(1575..1820)
                     /locus_tag="U4B03_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2069066.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1890..2144)
                     /locus_tag="U4B03_002246"
     CDS             complement(1890..2144)
                     /locus_tag="U4B03_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069067.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2308..2631)
                     /locus_tag="U4B03_002247"
     CDS             complement(2308..2631)
                     /locus_tag="U4B03_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069068.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2818..2891
                     /locus_tag="U4B03_002248"
     tRNA            2818..2891
                     /locus_tag="U4B03_002248"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2852..2854,aa:Ile,seq:cat)
     gene            complement(3276..3773)
                     /locus_tag="U4B03_002249"
     CDS             complement(3276..3773)
                     /locus_tag="U4B03_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2069069.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3813..4622)
                     /locus_tag="U4B03_002250"
     CDS             complement(3813..4622)
                     /locus_tag="U4B03_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2069070.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4956..5813
                     /locus_tag="U4B03_002251"
     CDS             4956..5813
                     /locus_tag="U4B03_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN2069071.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5806..6708
                     /locus_tag="U4B03_002252"
     CDS             5806..6708
                     /locus_tag="U4B03_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN2069072.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6741..7091
                     /locus_tag="U4B03_002253"
     CDS             6741..7091
                     /locus_tag="U4B03_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2069073.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7140..8063
                     /locus_tag="U4B03_002254"
     CDS             7140..8063
                     /locus_tag="U4B03_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2069074.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8141..9034
                     /locus_tag="U4B03_002255"
     CDS             8141..9034
                     /locus_tag="U4B03_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2069075.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9072..9635
                     /gene="amaP"
                     /locus_tag="U4B03_002256"
     CDS             9072..9635
                     /gene="amaP"
                     /locus_tag="U4B03_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN2069076.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9649..9837
                     /locus_tag="U4B03_002257"
     CDS             9649..9837
                     /locus_tag="U4B03_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN2069077.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9850..10752
                     /locus_tag="U4B03_002258"
     CDS             9850..10752
                     /locus_tag="U4B03_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2069078.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10784..11026
                     /locus_tag="U4B03_002259"
     CDS             10784..11026
                     /locus_tag="U4B03_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2069079.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11282..11653)
                     /locus_tag="U4B03_002260"
     CDS             complement(11282..11653)
                     /locus_tag="U4B03_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069080.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4176 a         1985 c         2232 g         3390 t
ORIGIN      
        1 attcattggt cgttgaatgg gctgaatagt agtgtataaa acaattttgt ggagcgtgaa
       61 gcttttatgg tagaggaaaa tacgaataaa gtgatgttga taaaaagatt cctatcttga
      121 atatgaatcg agatgtcttg aaaatgaatg agtttgacca atcattatcc tatcgaaatt
      181 tacagtttcg ttcgattggt ctgtcaatta atgaaaaagt aaaaaagaat atgttctagg
      241 aagagggcgg cacatctaaa tttttaagta acggacagtt caaatatcaa gcattgaaag
      301 aggctatcct actgtttgaa ggaatacgaa tatataaaaa cgacaaagag gaaaaagtag
      361 ttcattaaat cccttcaagt gatgaaagaa tgagatctaa taaaacctga tctttcatct
      421 gtttttttaa taacgtaata atttttttat tttttgtgct ttcgccgtta ttgtagagtg
      481 atttagcatt cttattttta aaaataactg tgttatatat taaaaaaggc ataatagtta
      541 taagcttttt taaagaatat ttagaaatta ttaattttcg tctgaaaaca aaaaaagaaa
      601 gcgttattat tttagtctat tttcaggggg ggaaccttat gataaaaaaa ctatttttac
      661 tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca actgaactga
      721 tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat cacagtgact
      781 acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt aagcatcgat
      841 gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag atcaaaagaa
      901 gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta aaccacgtca
      961 ggagatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag tacgctttta
     1021 gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct tgatctaaga
     1081 tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt atattaggta
     1141 agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac ttcatcgtgt
     1201 tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat
     1261 ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg tttttccatt
     1321 tcatccatga caagctgatg atattggaat aatctataag gagaatagtg aaaaacataa
     1381 tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca ttcccgatgt
     1441 tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca taatcgcata
     1501 gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa aaccagcacc
     1561 aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat taggtaatca
     1621 acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg aatttcttct
     1681 ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga aacagctact
     1741 tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa
     1801 atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt ataatgacta
     1861 tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg tatcaaataa
     1921 agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa aacactatcg
     1981 ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg aagagtcaaa
     2041 atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact tgcgaccagt
     2101 gcgaccgcag gcacccaaaa caaaactctc accaacgtaa tcatgtaacg gactcctcaa
     2161 cgattcatat aataaaagat aacatgttat tgcatacatc acaaatgaaa tgctaggaaa
     2221 ataaaagaag aatagatcaa tgaaaaatga aaaagcagaa cagttttctg ctgataagca
     2281 ggaactgttc cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat tgatatctaa
     2341 gtaattcaat acatataaaa agaggacgat cgcattcact aaaaaggaaa gaatgatgat
     2401 cccatagcga atggaaacat atttaacaaa tacttttgtt tgaatacggg catatctttt
     2461 tcctttatag ctgagcatca tgacaagaag caaaagggta atcacacctt gaaaaatcat
     2521 tccaataaac agaattcctg aggtttgata atttaattgg agaaaataag ctgctgccgc
     2581 aactaaatcg aataaaatcg caataaatgg caaaataatc actcctaaca ttgagaataa
     2641 ataaaagaat aacatttttt ctaaaataag caaataaaaa ttaaagtaac aaaatagtta
     2701 tgacacaagt aaaagaaata agaataacaa tattatttta cttatatttt gaaacttgtg
     2761 agagtttgtc attcgtgcta tagttgaaaa gtaacaaaaa taaattttat ttatgatgta
     2821 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
     2881 tacaaggtac ataagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
     2941 gtaaaaacag cttcttatgt ttttacttgg ctgttcttat tactgcagat gcaagctttt
     3001 gaataccgtt taaggaagag gttgtgaaat cagcgttttg acctgagtat tagcttaaca
     3061 aacggaaaaa tagttcctca tattttgccg tttgttaagc taataccgag ggtcagctga
     3121 ttgaacacca tttaagaaag aggtcgtgag cctgccgttt cactctgtgt cataaaaaag
     3181 ccaatctact ttgatagaaa aagcacgtag gactaaaaca gttctgcgtg ctttttttat
     3241 tgtgtgttaa atatcgtaaa tgaagataga tatttttatg attcgacgat agagacgttg
     3301 aaatctttca acagctctaa agcggattct ccgtgttttc tactggagac taacattgga
     3361 tcaacaaaaa agtctgcttc aggaaaagca ctctgcaggc ctatcagatt agagagtaca
     3421 cacaagtttg tttcaatccc gactacttcg atctcttttt gttcttcttt gctttcaaat
     3481 aaagtttcct ggatttcagc taaagtttca ggtggaatcg taaagtaata ctttttaatc
     3541 actcgttcat tttcttgtgg agaaagaaga gggatcaatt gcaaatcttc tcgttcttct
     3601 tctttgtcct tcagttcaac tggtatatcc cgtgtgaaga gaatatattc attattttgg
     3661 cgtgctttag ctaatctttg agcaatacgt tctgccaatt gatctgactc tggaatataa
     3721 aattcgctgt ttggatcaag aatatgattt tgcatatcaa taataactaa cattttcttt
     3781 cctcctacat tcatatcgtc attttattct ttttaataga agttttttaa aatataagca
     3841 acaccagctt cttcattcgt cagtgttact tggtttgcaa tttcttttac ttgtcgctgt
     3901 gcattcccca tcgcaatact caacccgctg taattcaaca tagcaatgtc gttttcttga
     3961 tcgccaaaag ccatgatgtc actcgattcg attccaaggt aatccgccag aaaaacaagt
     4021 gaatgagctt tagtgatacc tgtaggcgtg atttctaaga aatgcggatc agagaatact
     4081 gattggatac catctgcttg cagatctctt tgtagcttct gtagtttttc ttcactctta
     4141 tctgttaatg ctaatttaag atagctaaga gaaggatctt ccaacatatt taaaatttga
     4201 tcaaaagaat ggttctgtaa attgaaatgt tcataataag ggtcatcttt gactagattt
     4261 gattcatcga atgtcagctg atcatcttgg gtttctcggc tagcaatgat ggatattttc
     4321 gcttcttgag catagaagaa ggcttttctc agtgtatcgc tatctatttt tgaacgtctg
     4381 agaactttcg ccatttttcc atccttcatt tcagcaatca gtgaaccatt ggaagaaatc
     4441 aaatagccac cgattcctaa ttcggaaaaa atatctttag cagaaatgct gttccttccg
     4501 gtagcgacgg ttacgattcc gccattttca tcaaatttcc gaagtgcttg gatattttct
     4561 tctggtatct ttccatttcg atcaagcaag gtaccgtcta aatcaatcgc tgctagtttc
     4621 attttatctt cctactttct ttttacatat ctattttcct gttaccttaa gattaaatga
     4681 gaatttaaaa aaatgaaact aacttgcttt gtcattatga attttttcaa aaaacattct
     4741 taagaaaaag gtggctatat atagtagttt ttggtcaata cctttgaata aaaagccatg
     4801 aaaaacgaat agttagcgct taccgatgcg ttttgtgata aaaaaatttt aaaatattca
     4861 atgaaaacaa agcatatatt ttgctttaaa accaaatgag gaatattcta gatgtagaac
     4921 aggttagaaa aagattagaa aaaggagtga ttttgatgga gaagagatac ggcgtagttc
     4981 ctgtggtaac tgcttcagac gaaaattatg ctccttactt gagcgtgatg atcgcaactg
     5041 cattagaaaa ttgcaataaa acaagacgca taaaatttta tgtgattgac gatggtctat
     5101 cagaatatag taaagaagga ttggaagaaa cggtaaataa atattcaagt aatgcaagta
     5161 tccaattcct gacagtggaa aaagatatct acgaagactt cctagtcagt gaccatatca
     5221 caactaccgc ttacttaaga atttctttac caaacttatt agctaaagag gattataaga
     5281 aagtccttta cttagattca gatgtgttag tactagacga tatcgtaaaa ctatatgatg
     5341 agccgttaaa tggaaaaaca atcggcgcta ttatcgatcc aggccaagtg aaagctttgg
     5401 aaagattagg aatcgattcc gatgacttgt acttcaattc aggagtgatg gtgattgaca
     5461 ttgatcaatg gaataaaaaa gaaattacag aaaaaactat ccattacctc agtgaaaatg
     5521 gcgatcgaat catctatcac gaccaagatg ctttgaatgc tgtactttat gaagattggg
     5581 aacaacttca tccaaaatgg aacatgcaga cttcgctgat ttttgaacgt catccagcac
     5641 caaatgaaaa atatgaacgt cagtataaag aaggaaatga aaaaccttcg atcgttcact
     5701 ttacaggaca tgataaacct tggaatacgt tgaaagatca tccgtatacc aatttatact
     5761 taaaaaaatt agcacacagt actctaacga aagtaggcga gatcaatgaa taaaaaagaa
     5821 atagcagtag tcgcaagttg taacacaaaa ttcgtaccac atttagctgc attatttgtc
     5881 tcagtattag ataattgcaa cccgtctaaa tttgttcggt tctatgtgat tgacgatgat
     5941 atcgattttg aaagtaaaca gcttttacgc ttctcagtga aaaacgcacg aatgaactca
     6001 gatgtggaat tcttgaaaat caataaagaa ttctttacaa atgtggtaat cagcgatcgt
     6061 attccagaaa cagcttatta tcgtattgcg attccggaat tgttccgcgg cacagaagtt
     6121 gaacgtatct tatatatgga ttgcgacatg atcgctttac aagatatctc taaattatgg
     6181 cgtcttgact tcggtgattc aatcgtagca gctgttgaag acgctgggtt ccatcaacga
     6241 ctggaaaaaa tgaaaatttc agctaaatca atgcgttatt tcaactcagg tttgatgctg
     6301 atcaatgtga aaaaatggtt agatgaaaat attacgcaaa aagtattaga tttcatcgaa
     6361 cataatccag aaaaattacg attccatgac caagatgcat taaatgctat cttacatgac
     6421 cgttggttac cacttcatcc gcgttggaat gcacaaggct atatcatggc aaaagcaaaa
     6481 aaacatccaa cagcagcagg tgaacgtgaa tacgaagaaa ctcgcaacaa tccttacatt
     6541 atccattttt caggacatgt gaaaccgtgg agcaaggatt ttgaagggcc aacgaagaaa
     6601 tattacgaaa aatatgcagg gatgacggct ttccgttgtg tagctaaatt ccctaaatat
     6661 ccaaaatatg caaaagttca accaaaaaca gatgtagttg ttaaataata gaagaccaca
     6721 actgaagagg gggagtagga atggaaatta caatgtctaa aaatgcagta gagacgttaa
     6781 ttgaaaaggt tggggaaaat acaaaaatcg cactcgcgct tatcaatgat tccgatccat
     6841 ttctaaggga caaaggtgct tttgcaaaag gcagcttttt tcaaatcatt ccttttgttt
     6901 cggaatttgg agaatatgcg actaaaatcg agcatccatt attggacatc tatacatcta
     6961 aattagagca aaattatttc ggtaaacgat tgaacatgga cttcaacaaa cagcttgaca
     7021 gcttttcatt agaaaatgaa atcgcagtac tcgattataa tatcaaatta aagaactgct
     7081 tcttcagcta atagtttcga gaaagtccaa aaaggaggta tacattgaga tactactttg
     7141 tggatgaaga aaagaatgta atagaaacag atgaaaaaaa atataattgg ctcgtacttg
     7201 atagtgcaaa gcccgaagag attgagcaag caatcgaaga attcgagctt cctgatgata
     7261 ttttcgtagg aaccgaatat ccggaagaag tttctcggat ggagcgcctt tatgagacga
     7321 aactagagca tccattttct ttggtagtga ttaatctaga tcagtcagat aatcgtatcg
     7381 aaaaaaaatt aacgccaata tcttttgtac tttccgataa tctcttgatt acttgtatcg
     7441 atagaaaaac cgaatttgtg gatcggctgc tgacaaagca tggaaatcat ttaaactcgt
     7501 ttgaaaagat catcacgtat acattattag atatttacac ccattacgtg aaagaactaa
     7561 gagagatgaa aaagcgaatt gacgctttgg ataaagcagc tcgtaaaaca acggaaaatg
     7621 aagaactgta taaacaggca gatcttgaaa gagacatcgt atatatcgat cataccttaa
     7681 gagatcaacg ggaaacaatg gatcaattat ggaattctga aactttcatc aaacgtttag
     7741 acgatgaaca gctactctat gatgtccatt tgagacagcg acaaacagaa aaaatgatcg
     7801 agatctatcg agatttatta gagagtattg gtgatctatt caacgggatg atggacaata
     7861 atctaaatca tatcatgaaa tatctagatt cagctgcatt aatcatttcc gttccggcgt
     7921 tgttcgcagg gatttgggga atgaatacag gaggattacc gggtaaaagt tctgctctag
     7981 gctttatact cgtagtcttt ggctctttga tagcagcagt agttttaggt taccatttat
     8041 accggaaaga ttatacaaat taaagaatct atttaaaaca ggtatcaaac aaagaaattt
     8101 gttttggcaa tacaatttaa aaactaggag gaatacagca atgacagaac caaaattaat
     8161 cgacccgcga aacttgtacc atacggagaa atttcctaaa caagatcagg atacaccagc
     8221 tttacagcag aatatgattc ctgttcctga ttgtggagaa gaatcctatg aaggcaataa
     8281 tcaactagaa aaccgccgtg tactaatcac tggtggagat tcagggatcg gtcgtgcagc
     8341 agcgattgca tttgctagag aaggggcaga tatcgctctt caattcttcc caggagaaga
     8401 aaaagatgca gaggaagtag caaaatacgt gaaagatgca ggacgtaaga ttgtcttact
     8461 accttttgat cttcgtgacg aacaagcacc taaagaaatc atagataaag ccgtgagtga
     8521 acttggcggg ctagatacga tggtactaaa cgcaggacag caaatttctt gtcagtctat
     8581 tgctgagtta ccacttgagc aagtcaaaga cacatttatg ataaacatca tcgctatgtt
     8641 tgctttagtg aaagaagctg ttccgcatat cccagcagga ggagcaatcg taacaacaac
     8701 ttctgttcaa gcattcaatc caagcgaaca tttattagat tacgcagcaa caaaagcttc
     8761 aatcgctaac tttacgatcg gtttagcaaa acagcttgca cctaaaggaa tccgtgtaaa
     8821 tggtgtagca cctggtccaa tttggacacc attacaacta gacgaaggac aaccttctga
     8881 ttcgatccct gaatttggtc agcaaagttt actagaacgt gcaggtcagc caggagaatt
     8941 agcaccagtt tatgttttct tagcatcgaa taaagcaagt tatgttacag cccaaattta
     9001 cggagtaact ggtggagaag cgatcaactt ataagaagtt gatcgtttca cccctatgag
     9061 gaggtgcgaa aatgaataaa ggtgtaaaaa cgatcggagt gatcggaaca ttacttttgc
     9121 tatctgtgct tttgtttgcg atactattaa gtgcaaccta tccattgcct tttcagctag
     9181 agaagtttcg attcttttcc atcactaatt catacgtgca acaatatgta ttttggcttg
     9241 ccgttgcatt tgctattttg acaattatca cgttacttgt tttcctgttt tatccaaaaa
     9301 caatcggaaa ctttctactg aaacatggtg atggggagtt gacgttggat aaaaaagcaa
     9361 ttgaaggaat ggttcgttct cacttgcatg aagatgaatt catccattca ccaaaagtaa
     9421 atgtcaaagc aacgaaaaac aagatcgccg tcaatgtaaa aggggagttg aaacgaactt
     9481 cagcactcat tggtaaaaca ggaacattga tgaaagaaat cgaaaatgaa gtacaacaca
     9541 tattgggaac aaaagagcca gtgaaagtat cggtgaaata cacaggcttt gaagaaccag
     9601 ctaaacgaag caatgaccgc aaacgagtag aatagggggg gaccaaaaat gaaagaacta
     9661 ttttcgacat acaagctccc tatcatcttt ggggtacttg gattgatatt agccgtttta
     9721 tggatcaccg tgggcttttt caagacctta ttgatcttga tcatggcagc gatcggcgtc
     9781 gctatcggtt tttatctgaa acaaacgaaa atcttagatc actattttaa atcttaggag
     9841 gaaacatcca tgaataacaa taacaaaaat aataacaata aaatgaacaa ccaaaaacca
     9901 gtgtctgcac atccagaccc aacgacacca aaccaatcaa atggtgtgaa cgatgaaaag
     9961 aacaaaacag caccagtatc tgcacatcca gatccagtag tccctaacgt tcaacaagga
    10021 gtagaagaga agaaacatga tgcagaaaag catgcacctc atcatccagg aactccagaa
    10081 tctaaagttc cagaacaaca tgatgcaatc gatccttcta aacctgtgga agagcaaaaa
    10141 catccttctg accatgcgaa agcacaagaa aaacttagtt ctgaccacac aaacggacaa
    10201 ggaaaacttg gttctgatca tacaaataac caaggaaagc atgattctga acaggaagat
    10261 tcaatgcgcg gtgagctgaa ttatgaagat aaagttgtgc aaaaaatcat cggaatcgca
    10321 attgaacaag tagacggttt attatctgca aacggtggtt tcttctctaa tgtggcagga
    10381 aaacttgtga atacagataa tgtaacggct gggatcgaaa cagaagtcgg taaaaaacaa
    10441 gtagcagttg atatggatgt cattgtggaa tatggcaaag atatcgaaaa aatctttgaa
    10501 gaaatgcaag aagtaatcgg caaagaagta aaaaatatga cacatttaga agtgatcgaa
    10561 gtcaacgcta acgtagtgga catcaaaaca aaagaagaat ttgaaaaaga tcaagaaacg
    10621 gtgcaagaca aagtaactga cgcagcgaaa aaaaccggtg aatttgcttc gaatcaaaca
    10681 gataaagtaa aatcagcagc tggaagcggc gcacaaaaag taaaagaaaa tacagaacca
    10741 cgggttcagt aataaggaag taaaacatta ggaggaacga aacatgggat ttatttggtc
    10801 acttatcgtc ggaggaatta tcggagcaat tgcaggagca atcacgaata aaggttcttc
    10861 gatggggatt attgcaaatg tcgtagcagg tttagttgga tcagcacttg gtcaagcatt
    10921 attaggttct tggggaccaa gtattgcagg aatggcaatc gtgccatcat tgattggtgc
    10981 catcatttta gtactaatcg tatcattctt tgctcgaaaa ctgtaaaaag acttttaaaa
    11041 agaggtcgtg aaaaagctgt tcttcatcaa gtaataagaa cagccaaaca aaaatagctt
    11101 ctcatatttt tcatttggtt gggcatatta tcgaagatgc aagcttttga acactgttta
    11161 tacggaataa gaggctgtga caagactttt gtcacagcct cttatttatg cgggcaaatt
    11221 cccaattaga tttttaaaag tttttcctta aatgtgtctt tattgataca gagaagcttc
    11281 tttaataaaa caatgcctcc ctaaattttc ttgagatagg aaaggtgaac atacaaaata
    11341 atacgaccac tttgaaaacc cagatgatcc acaaatggat cgtatgatac tgatgaagta
    11401 aaaagcaatg gatcagaaac tcaaagagat tgaaaaagac aaggaagaaa taatatctct
    11461 tttttactct tgtctgtttt agccaataat agctcgtaaa gtaatctaga caaacaaata
    11521 gaagtaaaca aagtttcgta agagcagcaa tcgtatcatg ttgctctgct agacgattga
    11581 cgagcaggaa aaatgttggt aatggaatat agatcacgag taatgaaaga aaaaagtaaa
    11641 caataaatgg catactattc ctcctaattg tgtgagttcg ccctcatttt aaaataacga
    11701 tatttgtttg tgaagctttt tcgtgtcgta acaaatgtta tctaatgtat ttttaaataa
    11761 ataatcatta ttatcaaaat aat
//
LOCUS       DASJSZ010000083        11706 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.087, whole genome shotgun sequence.
ACCESSION   DASJSZ010000083 DASJSZ010000000
VERSION     DASJSZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.087"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..1455
                     /locus_tag="U4B03_002261"
     CDS             127..1455
                     /locus_tag="U4B03_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN2069081.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            1657..2490
                     /locus_tag="U4B03_002262"
     CDS             1657..2490
                     /locus_tag="U4B03_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEN2069082.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2506..3243
                     /locus_tag="U4B03_002263"
     CDS             2506..3243
                     /locus_tag="U4B03_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEN2069083.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3398..4366)
                     /locus_tag="U4B03_002264"
     CDS             complement(3398..4366)
                     /locus_tag="U4B03_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEN2069084.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            4598..5431
                     /locus_tag="U4B03_002265"
     CDS             4598..5431
                     /locus_tag="U4B03_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEN2069085.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5465..6304
                     /locus_tag="U4B03_002266"
     CDS             5465..6304
                     /locus_tag="U4B03_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2069086.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6330..6890
                     /locus_tag="U4B03_002267"
     CDS             6330..6890
                     /locus_tag="U4B03_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2069087.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7089..8810
                     /locus_tag="U4B03_002268"
     CDS             7089..8810
                     /locus_tag="U4B03_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEN2069088.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            8975..10117
                     /locus_tag="U4B03_002269"
     CDS             8975..10117
                     /locus_tag="U4B03_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2069089.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10133..11344
                     /gene="thiI"
                     /locus_tag="U4B03_002270"
     CDS             10133..11344
                     /gene="thiI"
                     /locus_tag="U4B03_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEN2069090.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4004 a         1947 c         2466 g         3289 t
ORIGIN      
        1 catttttctt catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag
       61 agattgtatt tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat
      121 gattggatga aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca
      181 aatatcaaac agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct
      241 gaaaatgttt cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg
      301 aaagtggcta atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc
      361 cgtcataaga tgctgaatca attccattta aaagagtttg aattatcaca aaattacact
      421 tttttctggg ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga
      481 aaagaggcaa tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc
      541 ggccaatggg atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg
      601 atgccggaaa gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta
      661 ctgcgtaaag atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct
      721 attgaagaaa agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta
      781 ggaacacctc caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt
      841 gaccgaggac tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat
      901 tatgttagcg tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta
      961 gagatgctag gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa
     1021 actttcaaaa aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt
     1081 gatgtaggtc aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg
     1141 gatgatctat ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa
     1201 agtctgatga cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca
     1261 aaatggaaag tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg
     1321 agtgatgcct ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg
     1381 aaagagcaat taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg
     1441 ggtgctttag catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg
     1501 gcttaagcca gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct
     1561 ataattctaa ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga
     1621 cgaagtagaa taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct
     1681 gatctgaaat taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat
     1741 cgtataaaaa ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga
     1801 ttttttgaag cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat
     1861 acgtatatgc taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg
     1921 ggaaaagggg atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg
     1981 ccacaatttg gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt
     2041 gaagaagatt ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca
     2101 gaagaaacga atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt
     2161 gtattgaaat cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg
     2221 agtggtaatg tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct
     2281 tatgggaatc gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa
     2341 tttttagatg cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg
     2401 atttaccagt tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc
     2461 caagtagatc aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat
     2521 accaaaaagc atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct
     2581 tcgatgttag gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc
     2641 agtgaattcg tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt
     2701 aatcgtctcc ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat
     2761 aagtttttct gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa
     2821 atagaatctg ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa
     2881 gagatcattg ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt
     2941 gaaaagattg attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt
     3001 aatcagcatg ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac
     3061 tttttgacta cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac
     3121 gggaatgaga tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa
     3181 catgaaatcc gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa
     3241 taaaaaagag gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa
     3301 ttgtttctca aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca
     3361 gcctctcttt ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca
     3421 attcttcacc cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac
     3481 tattaatact gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat
     3541 aatcataaac aggctctgcg attccattga aacaacgaga aacaagtact acagggattt
     3601 tttggacaag aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta
     3661 aagcttcgat taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt
     3721 gcatcccagc atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc
     3781 gctttgtttc taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg
     3841 gacccaatgt aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc
     3901 gtgcagaatg gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac
     3961 aacttgcgac acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac
     4021 ttgaacgcat tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa
     4081 aaaaagcagt ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg
     4141 atgcataagc ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg
     4201 gtgagggaag attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt
     4261 ctgcatctaa taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt
     4321 tcatggcgat cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct
     4381 tacgaataat tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta
     4441 tcagtgaaga ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga
     4501 aaagactcaa ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga
     4561 aagcagccaa ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc
     4621 gtttaaagaa catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg
     4681 gtttctaccc ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt
     4741 tgagaattgg ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg
     4801 aatcagcggt caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca
     4861 ttttttagat gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca
     4921 attcccctca tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa
     4981 gtggtttgag ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga
     5041 atttcttgaa cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga
     5101 tgagccacag ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt
     5161 tgctcttttt cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg
     5221 gcgtaaaaaa agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga
     5281 agtcaaagta gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg
     5341 tggtgatgcg gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg
     5401 actcaatcca acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa
     5461 gtctatgatc aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa
     5521 agcgttggaa tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg
     5581 tgtagcaacg gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga
     5641 tatgtatgtc gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca
     5701 cccttttgaa caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca
     5761 aatcgttttt ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc
     5821 gatttttatc aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa
     5881 aaaaatcctc caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat
     5941 attaaaagaa ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt
     6001 agaacatcgc tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat
     6061 cccaaaaggt ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga
     6121 aatggaagaa attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt
     6181 aggaattgga gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt
     6241 gactcaatca aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta
     6301 ttagtcaatg aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag
     6361 aaacattcca caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa
     6421 atgaagctca tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta
     6481 cttcacctaa taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata
     6541 aagcagaaga aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag
     6601 tagatgcact gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag
     6661 tgcctgatcg aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg
     6721 gaaaacctca ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg
     6781 aatatgctcc agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg
     6841 ctaagaagaa atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc
     6901 actatttttg ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat
     6961 gcatgaaaat agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt
     7021 tggatttatg ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg
     7081 aaccataaat gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca
     7141 ttgtcgtctt tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct
     7201 tagaaaaacg caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta
     7261 aaaaaatgca tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga
     7321 atgacatatc tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc
     7381 tcaatgaaag ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa
     7441 tggaccaaat ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa
     7501 cggaagaacg caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag
     7561 aaaaagtcag agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga
     7621 tcaaagtgat cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg
     7681 ttgaagcccg cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga
     7741 tgaaaaaaat tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag
     7801 aaatcagtga tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc
     7861 ttgctgaaga tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa
     7921 aaacagaagt tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt
     7981 tatatgatat cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa
     8041 cgattgcaga gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg
     8101 atcacacatc tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc
     8161 aaaccgaagt ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac
     8221 acgaaattcc ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag
     8281 atatcgaaaa gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa
     8341 aagaagcgca agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg
     8401 tagaaaaaca acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta
     8461 ctgaccgtat tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg
     8521 tcaatcgttt agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg
     8581 atttagtaga tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc
     8641 atacacacga agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat
     8701 atcactatca agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg
     8761 ctttcaaacg catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag
     8821 ttagtaaacc agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt
     8881 tgaaaattga aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat
     8941 aatagaaaag aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac
     9001 aaccccaatc tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg
     9061 gaatccatca agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag
     9121 aaagcagatc gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg
     9181 aacagaagga gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa
     9241 tcatatgatc atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa
     9301 agaattagga atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga
     9361 cgaattgaaa gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa
     9421 tgaagtagga tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa
     9481 aattcatttc catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt
     9541 gacagaaaga gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac
     9601 tggtttcatt tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca
     9661 agaaagaaat cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc
     9721 tttacgttta tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt
     9781 agctctaatc aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga
     9841 ctatgcaccg catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca
     9901 tgccttagaa gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca
     9961 tatggcaagc agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt
    10021 tcgaatcagc ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt
    10081 caagcagctt tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata
    10141 cagtgaaatt atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt
    10201 tatcatgcaa ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat
    10261 ccatgctgat cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat
    10321 tccaaaacta gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa
    10381 agatgtcgaa gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa
    10441 agaaacattt aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga
    10501 attgaatcaa acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat
    10561 gcgtcaacca gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta
    10621 tgaaacgatc aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat
    10681 gctttctggg gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga
    10741 aattgaagct gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc
    10801 aaaagatcta acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc
    10861 atttaccgag attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat
    10921 tactcgtcga atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt
    10981 cattttgaat ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc
    11041 tatcaatgaa gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga
    11101 aatcattgaa gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga
    11161 ttgctgtacg atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca
    11221 tcaatatgaa gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa
    11281 gatggaagag atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct
    11341 ataagaggtt atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata
    11401 gctccatgta ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt
    11461 atcaagaagt tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta
    11521 gagatcaagc tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg
    11581 ttgtgacaga agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa
    11641 atttttgtga aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag
    11701 aggttg
//
LOCUS       DASJSZ010000084        11410 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.088, whole genome shotgun sequence.
ACCESSION   DASJSZ010000084 DASJSZ010000000
VERSION     DASJSZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.088"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(122..607)
                     /locus_tag="U4B03_002271"
     CDS             complement(122..607)
                     /locus_tag="U4B03_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEN2069091.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    697..1042
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1087..1728
                     /locus_tag="U4B03_002272"
     CDS             1087..1728
                     /locus_tag="U4B03_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2069092.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1747..2382
                     /locus_tag="U4B03_002273"
     CDS             1747..2382
                     /locus_tag="U4B03_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2069093.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2400..3215
                     /locus_tag="U4B03_002274"
     CDS             2400..3215
                     /locus_tag="U4B03_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2069094.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3534..4970
                     /locus_tag="U4B03_002275"
     CDS             3534..4970
                     /locus_tag="U4B03_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2069095.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5159..7591
                     /locus_tag="U4B03_002276"
     CDS             5159..7591
                     /locus_tag="U4B03_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN2069096.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            7821..8888
                     /gene="rlmN"
                     /locus_tag="U4B03_002277"
     CDS             7821..8888
                     /gene="rlmN"
                     /locus_tag="U4B03_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN2069097.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9081..10184)
                     /locus_tag="U4B03_002278"
     CDS             complement(9081..10184)
                     /locus_tag="U4B03_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2069098.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10736..11284
                     /gene="aac(6')"
                     /locus_tag="U4B03_002279"
     CDS             10736..11284
                     /gene="aac(6')"
                     /locus_tag="U4B03_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN2069099.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3783 a         1934 c         2465 g         3228 t
ORIGIN      
        1 tagttttcag cgttcatact agttgttaca ttgtttgatt ggccaaaaga atttctgata
       61 tattttgtgc taaataaaga aaaagacgct tgaattggga aataagaaaa ctactctatc
      121 attacaaatt ttcataaact ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg
      181 aaaaatccat aatcaacgta gtggtattga tatttggatg aaagcctacg gtatcttgac
      241 gatcaatagc agcatcgatc acgacttgga tttttttctc tttgtcgtgc atcaatgcta
      301 atggagtcac tgtacctgct ggaacgccta ggaggtcagg aagctgttct tcagaaacaa
      361 aagataagcg cttttctttt aattgttcag ctagttgttt taaatcggcg tgcttttcat
      421 caggcaagat gacaagatag atttgttttc cttttcttga tttcaataat agatttttta
      481 cttgcggacc aggtagggaa aaagggacat ttttgactga cgtaatcgca ggatgatcga
      541 ctttttgaaa agagataccc agttttttta gtagttcata agcttcttgt tcggttgcac
      601 gactcatcgc gcatcaccct ccttttttta ttagtgtatc ataaaaaaga gttgacaaat
      661 gaaaaataat catgtaaatt acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta
      721 gccgttaggt gtttttagag agtctctggt tggtggaaag aggcagcagt taaccgtgaa
      781 acacatcttg gagtcgattt tctgaagcgt atagtaggaa aatccgggga gcccgttaca
      841 gctgcggtcg ttgctagatg accagataag gctggctttt ccgtgaggag tcggtaaaaa
      901 aaggtggaac cacgtgatta acgtcctttg atcttcggat caaaggactt tttgttttca
      961 tctgaacggc aacgactgtg tgaggttttt tctgcaagga aaaaataaac agaggtggaa
     1021 ccacgtataa tacgtcctct tggcatttgc caagaggatt tttttatttt aaaggaggaa
     1081 aagaagatgg agtatttgat tgaagttatg ccgcaattat tagtaggagc actcacaaca
     1141 ttgaaattat ttttatttac cttgattggc tcattaccat tagggattgt tttagcatta
     1201 ggattgtctt tgcattttaa gccgctacgc tacttgcttc aaacatatgt atgggttatg
     1261 cggggaacac cgctgttgtt gcaactgatt tttatttttt atgggttgcc gacgattggg
     1321 atcgtttttg atcgtttcga agcagcactc attgccttta tcttaaacta tgcagcttat
     1381 tttgcagaga tctttcgtgg agggatacaa tcgattcctc aaggtcaata tgaagcagcg
     1441 caagttttgc aattaagtcc ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca
     1501 aagattgttt taccttctgt agggaatgaa gttatcaact tggtcaaaga ttcttccttg
     1561 atctatatat taggtttagg tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac
     1621 gcgactttac tgcctttgct aggcgtggca atcatctatc ttacattgac tggtattcta
     1681 acaatcgtaa tgaaacaagt tgaaggaaaa ttaaattatt accgctaact gataggagga
     1741 agaaatatga ttgaattgaa gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa
     1801 gatttgaatc taatcattcc agatgggaaa acattagcta tcgtaggtcc ttccggtggc
     1861 ggtaaaacca cgcttttacg tatcttggct ggcttagaaa gttctgacag cggagaattt
     1921 tatttagacg gtgtaccgtt caacccattc gagcttaaaa tgaaagaaca agttgtagga
     1981 gtcgtattcc aagacttcca gctatttcca catctgaata tttttgaaaa cattacctta
     2041 gcaccaaaaa tggtattgaa acagacaaaa gaagaataca ccaaaaaagc agaagaatta
     2101 gcggcttcat tagggattag tgatttattg aatatctatc cttatcagct ttctggcgga
     2161 caaaaacagc gggtagcgat tgccagagca ttggctatga atccacgggt attggcttat
     2221 gatgaaccaa caagtgcttt agatcctgaa ttacgccagc aagtcgagcg tttgatccta
     2281 tcattgaaag aagatggggt aacccaaatt attgtcaccc atgatttagt atttgcagaa
     2341 aatgtcggtg atcaattact aaaagtttcc ccaattcatt aagaaaggag aagatagaga
     2401 tgaaaaaaac cagttttcta ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt
     2461 cgaacaaaaa aacttcagca gataattggg cgactatcaa cgaagagaaa aaaatcgtta
     2521 taggtttaga cgatacattt gttcctatgg gatttagaga taaagacggg aagctcacag
     2581 gatttgatat tgatttagcc aaagcagtct ttgaacaata tgggattacg gtagactttc
     2641 agcccattga ctggtcaatg aaggaatttg aactgaacaa tggaacgatc gatttgatat
     2701 ggaacggcta ttccaaaacg ccggcaagag aaaagaaagt gcaatttaca aaaccttata
     2761 tggaaaacga tcaagtgctg atcacaccta aagaatcagg aatcacaaac tttggacaaa
     2821 tgaataataa gagattaggt gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac
     2881 aaccagaagt attaaaagag atagtcaaaa accaagatgc tgttttgtat gatagtttta
     2941 atgaagcatt gatcgatctg aaaagcagac gggtcgatgg tctgctgatg gataaagtat
     3001 acgcagacta ttatttgaaa caaagaaacg aactagaaga cttcaacata acaaaaggtt
     3061 cttatgagag tgaggatttt gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa
     3121 aaatcaatga agccctagat caactagaga aaacaggtga attccaaaaa atatccgata
     3181 aatggtttgg cgaagatgtt acgccgagaa attaacaaat aggggactgt gacaaaagac
     3241 gtgtcacagt ccctttttcc gaataaacag tgttctcctg ccaactcctt gtgactcgga
     3301 gctgaatggc aggctcacaa cctcttggta aacggtgttc aaaagccagt ttttcggtat
     3361 taagccaaat ttatgcaaaa atgtagaaag ccattttcac aaaatttttc ttactactcg
     3421 aaactaagcg actttttcac aacctccttt acataatttt attttgttgt ggaaggggtt
     3481 tcgtgttatt ctaaaagagg aacgattgaa ggttatagga aaggaagaga tgaatggaac
     3541 acaaagaact tacctcattt ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc
     3601 aagtagaagg agcttggcaa gaagatggga aaggagaatc agtctgggat cgatttgtac
     3661 ggatacctgg aaagacattt aaaggaacga acggtgattt ggcagtagac cactatcatc
     3721 gttataaaga agatattgct ttaatgaaag aacaaggtct gaaagcttac cgcttctctg
     3781 tagcttggac acggattttt cctaatggac gaggagaagt caatcaagct gggctcgctt
     3841 tttatgagag attgatcgac gaattgattg aaaacgatat tgaacctatg ttgactttat
     3901 atcattggga tcttccacaa gcattgcaag atgaatataa tggttgggaa tcacgtcaga
     3961 tcattgaaga tttcactcat tatgcagaaa cattatttga ggcattccgc gggaaagtcc
     4021 attactggat tagtttgaat gaacaaaata tcttcacttc attaggttat ttgttagcag
     4081 cacatccgcc aggtgtgaca gacccgaaac gaatgtatga agttaaccat attgctaatt
     4141 tagcaaatgc atctgtcatc aataaatttc acgagttaga aatgcctgga aaaatcggac
     4201 caagctttgc gtatacgcca aattatccaa ttgacagtaa tcctgaaaat gttctggctg
     4261 ctgaaaatgc agaagacctg atggccaatt attggatgga tgtctatatg tggggaaaat
     4321 atccgattgc tgccatgagg ttcttagaag aaaaaggatg ggcaccaacg attgaagctg
     4381 gcgatgcaga attgctagaa tctgctaaac cagattttct tgggatcaat tactatcaaa
     4441 cggcgacgaa tgcattcaat ccattaaacg gagttggtgc tggaaaaatg aatacgacag
     4501 gaaaaaaaag ttcatcagaa gaaactggag ttcctggaat gtataaaaaa gttgagaatc
     4561 catttgtcga acgaacaaat tgggactggg agattgaccc agaaggatta aggatcggac
     4621 tgcgtcgtat cacaagccgt taccgcatgc cggtactgat tacggaaaat ggcttaggag
     4681 agtatgacaa gttgacagaa gataaacaaa tccatgacga ctatcgaatc aattacttac
     4741 aaagccacgt caaagccatc aaagaagcaa tttcggatgg agcagaagtc ctaggctatt
     4801 gcacctggtc ttatacagat ttgcttagct ggctgaacgg ttatcaaaag cgatatggat
     4861 ttgtatatgt tgatcaagac gaaacacaag aaggatcttt ggaacgatat aagaaagaca
     4921 gttattactg gtaccaaaaa ctgattgaag aaaatggaga gaacctttag ttcacatact
     4981 taaaacaaga aggtacggct ggatatggcc gtgccttttc ttgataaaat catttagaaa
     5041 ttaagaaaaa ctgtaaaata agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa
     5101 actttagttc ccttactttt ttttgtataa tgaaacttga tgaaatgagg gataataaat
     5161 ggcacaaaag aaacggccta ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa
     5221 acagcagcag gaacaaatgc tgacgattgg tgttgggttt gccttgattt tatttgctgt
     5281 cttcggcttt ttaaaattag gttttttagg aatattgatt gcaaacggct ttcggatcat
     5341 tgcaggaaat acttaccaaa tcctctgtct tttattagcc gtattaggat tttggattgt
     5401 cattaagaat acagagtttt caattggtaa aaaccgacgc tggtttggcg gcatactctt
     5461 ttatttcggt atcttattat tgttacatgc ccatttattt ggcaaactcc atactgggga
     5521 accaaatatc atggggacga catgggattt gttagccagt gatataaaac aaagtcaagt
     5581 agacaataac gttggtggtg ggatgattgg agcaatcctt tatcatttta cttacttctt
     5641 gattgcacag cctggaagtt atttagcagc agtgctatta ctggcaggag gagcattcct
     5701 gttcagtaat ctggaaggct atcagctgtt aaatggtatc caatcaattg gagaacgagt
     5761 acaggaatta ttggaaggtg atcctgccaa gcaagctcga aagcaagcgg caaaggaaga
     5821 gagaatgaaa cagcgcgcag aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc
     5881 agaatatgag aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca
     5941 ggaaccagaa aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga
     6001 aaatatccat cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac
     6061 agcagaaggt ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat
     6121 tgaagcagaa caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat
     6181 accaactgtg gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga
     6241 acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc
     6301 cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt
     6361 aacagatgat atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc
     6421 aggtaaatca ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag
     6481 agaaatcatc gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg
     6541 tgatgtttct ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc
     6601 tggttcgact ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat
     6661 gcgtgctaag ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa
     6721 tatgtacaac ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc
     6781 gcaagcctta caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac
     6841 cggagtaaga aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg
     6901 aacgaaacat ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat
     6961 gatggtagcc agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc
     7021 agcggggatt catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat
     7081 catcaaagca aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg
     7141 gacgatcatt gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt
     7201 gccaatgggt gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt
     7261 agaacgggtc gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat
     7321 gccgacagat gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga
     7381 agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag
     7441 atttcggatc ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt
     7501 tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc
     7561 agaaacgttg ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga
     7621 ataaccaata aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct
     7681 tttctaatac ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga
     7741 tttatttgtg aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg
     7801 agaaaaagag ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat
     7861 taatcaattg gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat
     7921 ggttgtatca aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga
     7981 tcgaaaaatt atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag
     8041 cttcggatgg tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg
     8101 tactgatgag acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata
     8161 tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag
     8221 aaattgttgc acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac
     8281 gcgtttcaca tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa
     8341 tggacttttt gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta
     8401 cagtttccac tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag
     8461 tcaatctggc tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga
     8521 tcaaccgaag tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa
     8581 caaaccgtcg aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc
     8641 atgcgcaaca attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga
     8701 ttccatataa tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat
     8761 tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg
     8821 gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg
     8881 ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt
     8941 aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt
     9001 ttgacaagaa aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg
     9061 atgggggttt tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta
     9121 gcatgataag tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa
     9181 ggcacatgtt taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc
     9241 gtgacattga gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca
     9301 tagattttat caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa
     9361 ctgacagatg ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag
     9421 aatgagtgtg ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta
     9481 gtaacaatga tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta
     9541 tctttcaatg cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg
     9601 attcctaatc tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact
     9661 tctttggata aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata
     9721 attgctgtat caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt
     9781 acgccttctt cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata
     9841 gtgggaaagg tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa
     9901 gcacggccac caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc
     9961 aactgttcaa tatttccata agttgaatta cccccgtacc agagactggc gacaacctct
    10021 atttcctgtg tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct
    10081 ccaatcagca ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca
    10141 tctgttccaa tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt
    10201 cttcgttgaa acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga
    10261 gtagaggtga acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc
    10321 atcaataagt atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt
    10381 tttgttcctc ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc
    10441 taaaaattaa gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt
    10501 ttaaagcaaa agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg
    10561 gctttcctga tctttcctat tttgaataaa gtgtttctgt ataatttttt attttataag
    10621 taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa
    10681 catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat
    10741 aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag
    10801 actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa
    10861 tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc
    10921 aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg
    10981 acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag
    11041 aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca
    11101 aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca
    11161 tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa
    11221 tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca
    11281 ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga
    11341 aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa
    11401 caccgtgtac
//
LOCUS       DASJSZ010000085        11241 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.089, whole genome shotgun sequence.
ACCESSION   DASJSZ010000085 DASJSZ010000000
VERSION     DASJSZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.089"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..1042
                     /locus_tag="U4B03_002280"
     CDS             5..1042
                     /locus_tag="U4B03_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2069100.1"
                     /translation="MKENAWDSLPEHLYKRHIDNVLILHGKTSWKAVKDVFPDLSITK
                     IFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKLIAYKTNLPIIILPTL
                     AATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHSPREMMIAGIGDTLAK
                     WYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAMDQQKINQAFIDVVET
                     NILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKVAYGILVQLMIENKLA
                     EIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEMIHFMKETITPALVVQ
                     KMKELEQVTQG"
     gene            1376..2314
                     /gene="yidC"
                     /locus_tag="U4B03_002281"
     CDS             1376..2314
                     /gene="yidC"
                     /locus_tag="U4B03_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN2069101.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(2495..2899)
                     /locus_tag="U4B03_002282"
     CDS             complement(2495..2899)
                     /locus_tag="U4B03_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2069102.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(2915..3724)
                     /locus_tag="U4B03_002283"
     CDS             complement(2915..3724)
                     /locus_tag="U4B03_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2069103.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(3711..4601)
                     /locus_tag="U4B03_002284"
     CDS             complement(3711..4601)
                     /locus_tag="U4B03_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2069104.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(4614..5093)
                     /locus_tag="U4B03_002285"
     CDS             complement(4614..5093)
                     /locus_tag="U4B03_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEN2069105.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(5110..6876)
                     /locus_tag="U4B03_002286"
     CDS             complement(5110..6876)
                     /locus_tag="U4B03_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEN2069106.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(6922..7863)
                     /locus_tag="U4B03_002287"
     CDS             complement(6922..7863)
                     /locus_tag="U4B03_002287"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEN2069107.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(7860..8855)
                     /gene="lacD"
                     /locus_tag="U4B03_002288"
     CDS             complement(7860..8855)
                     /gene="lacD"
                     /locus_tag="U4B03_002288"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN2069108.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(8848..10041)
                     /locus_tag="U4B03_002289"
     CDS             complement(8848..10041)
                     /locus_tag="U4B03_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2069109.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(10059..10787)
                     /locus_tag="U4B03_002290"
     CDS             complement(10059..10787)
                     /locus_tag="U4B03_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2069110.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         3220 a         2437 c         1986 g         3598 t
ORIGIN      
        1 tgaaataaaa gaaaacgcat gggattcact ccctgaacac ctttacaaaa ggcatattga
       61 caacgttttg attctccatg gaaaaacatc ttggaaagca gtaaaagatg tttttccaga
      121 tttatcaata acaaaaattt ttgaatatta tggtggagaa tgtaccgatc cacttgctga
      181 cagttttgtt tctttatgca aaaagaagga tttcaatggt attattgcag tcggcggcgg
      241 gaaagtagca gatctaggaa aattgattgc ctataagaca aacttaccaa tcatcatctt
      301 acctacctta gctgcgacgt gcgcagctta tactccactt agtgtggtct ataatgaaga
      361 aggagtgatg cttcgctatg atatgttccc taaagcaaat gatttagtgt tgatcgatcc
      421 aaagatcatt ttgcactccc ctcgcgagat gatgattgcc ggtattggtg acaccttagc
      481 aaaatggtat gaatctcacg caatcatctc tcagttaact atcaagccta tcgaagtcca
      541 agtagcggaa tttgcagcgt tacgatgccg cgaaatcctt ttagagaaaa gtgcagaggc
      601 aatggaagcg atggaccagc aaaaaatcaa tcaagctttt atcgatgtcg tggaaacaaa
      661 cattttattg gctggtatgg ttggtgggtt tggcgatgac tacggccgaa cagctggagc
      721 tcattccatt cacgatgcga tgaccgtact ccctgatagc catcagcaac ttcatggaaa
      781 caaagttgca tacggtatcc ttgtccagct gatgatcgaa aataaattag cagaaatcga
      841 acacctactg ccgttttatc atcacttgaa cttaccaacg agtttagctg atatgggttt
      901 acatttaaca gaaagtgact atcaagccgt agctgaacgt gcatgtttac ctgatgaaat
      961 gatccacttc atgaaagaaa cgatcacacc agcactggtt gtccagaaaa tgaaagagtt
     1021 agaacaagtg acacagggat agttagcccc ttataccatg gcagtcagct tttttatgga
     1081 tcggctcaat ggtaaaaaaa aagaagtgga aaaaattgac attccacttc ttttttttgc
     1141 gctaaaatta tgatttcaag gaaatatccg ttgctcatct tattttagag aacggaggtg
     1201 ctaagtatgc tttatactga agtattttta gctggattct tctttttaac attaagcgtc
     1261 ttttttgatc ctccaatcgg ttatttctat gaaatttcta tgaagttgat tgaattatga
     1321 gcgctttttt agtatagtta agcttgtgta aaaattataa aagaggaagt attttatgaa
     1381 taaatggaaa aattggttat tgggctctgg gcttgtcacg atcatgcttt tcttgtccgg
     1441 ttgtgtccgc atggattcaa atggtaaccc tgacacttca ggaatcattt accgtatatt
     1501 ggttcaccca atgggacaag cgattacata tttagtggaa aactttaatt ggtcctacgg
     1561 ctgggctgtt atcttgatga cagtcatcgt tcgtatcatc attttgccat taggtattag
     1621 tcaatcgaaa aaaacaatga tccaatctga aaagatgcaa gcattgaaac cacaagttga
     1681 agctacgcaa caaaaattga aagctgcgac aactcgagaa gaacaaatgg ctgcacaagc
     1741 tgaaatgcaa caagtttatc gtgaaaatgg cttaagtatg actggtggta tcggttgttt
     1801 accgcttctg atccaaatgc cgatcttttc tgcgctatat ttcactgctc gctatacaga
     1861 aggcatccgt gaatcaacct tttacgggat cgacctaggt aaccctagct tagttttagt
     1921 agcgattgcc ggtattgctt atttacttca aggctatatc tcaactatcg gtatccctga
     1981 agaacaaaag aaaacgatgc gtacgatgtt gatcgtatca cccgctatga tcgtcttcat
     2041 gtcgatcagt gccccagctg gtgtcactct ttactgggta gtcggtggga tctttagttg
     2101 tctacaaaca ttcattacga atgtcatcat gaaaccaaga ttaaaagcgc aagttgcgga
     2161 agaattaaaa caaaatccgc caaaacaagt ggtgacgcct cgtaaagatg tcacacctgc
     2221 tgaacctgcg aagcaaacaa aaaaacaagt acaagcacca aaaaacaatg cgaagaatgg
     2281 tcggaatgct ggtaaacagc aaaagcgtaa atagttattt gtaactagct tcttcgatcg
     2341 acccagatca tttctgattt tatgacgata aaagccaacg tactgtgaaa tatagaaaac
     2401 tatatttcgc ggtacgttgg cttttttata cacggcctct ttatacatcc gtatttatat
     2461 ccagttgtat caccagtcaa gaagttgtgc ttgattattc ttcttcatcg tctcccatgc
     2521 cagcaatcac ttggttcact gaaacgatac tctcacgacc cgaaccaata aaatctggtt
     2581 ctgctccaat catttgcgaa ttgatgaatt cgatgaccat cggcaaattc atgccagcat
     2641 atagttcgat cgtttcacct tccatgatta gcttggaaac ggtatttgca ggtgtcccac
     2701 ccaacagatc gcaaagcact acgtaatctt tcaatggtgc tgtaacggag aggaatcttt
     2761 ctttatacgt gtccgcgcca tcttcaggca ataaagggat cgtatagata ttttcttgtg
     2821 gtcccatgat catttctgta ctgtttttta gttcttcaca aaatttacca tgactaatca
     2881 agactagttg tctactcatc tttccaattc tcccttattt cgaaatcaca cctaatgcag
     2941 ataatgctac acatacgatc aatacgatga atattgctcg agtaggtgtc atatttttct
     3001 tacctaacat ccaataaacg ccaccaacga ttccagctgg taaaagttta ggtaagatca
     3061 tatcgatata gttttgcata ttcaacgtta catccccaat cgttggtgcc catgagaatt
     3121 taacattgat catggttgct actaacgctc caaccatgaa aactcctagt aaagtagccg
     3181 catctgtcaa tgcagtcaag cgtgattgca tcgtggtaac tagagaaatc ccttctttgt
     3241 aggcaaattc tagttgtttc caacgaaaga ccatgacagc gacctgagca aggatccaga
     3301 taaggatacc tactggatta ccatctgttg ccatatttgc ggctaaagct ccaaatatgg
     3361 tcgggatcaa tgccgcaaat acagaatcac caattgcagc gaaggaaccc atcaatcctg
     3421 ctttcatccc agaaacagtt tcctttgaag agactccttc tttttcttcg atagcaagat
     3481 cgatcccagt aatgatcgtg ttaaagaaat tagacgtatt aaaaaattgc gtatgtgttt
     3541 tcatcatttc ttttaattct ggtgtatcat caccatagat ttttctaagt tgtggcaaga
     3601 tagtatacag ataacctgaa ccttgcattc tttcatagtt ccaacctaat tggaaagtaa
     3661 acaaacttct acgattgatc tgcttaaaat cttctttggt caatttataa ttagatttcg
     3721 tcatcttcga tttccccctc atcagattgt gattgctgac ttactactgg atgttggtca
     3781 ttcttttgat cattcttata actgatcgtt gccagtgcta gaccaatcaa tgccacagca
     3841 agcatcggta atgaattgaa tgttcctggg aaatctttga ccacgctagc cacacttgtt
     3901 cctaacaatt ggacattcga gaacactgtt gctaataacg ctgtgatcgt aaatcctaaa
     3961 atcagataag aaatgtgttt ctttactggt aggtagcgta gtaaaattgc aaacccaact
     4021 gctggtaaga ctgcccctgc aacagataag ccatctccta gccattttag gtcgccgttt
     4081 aaaacagcca caacattttg gactaagcca ccaccgaatg ctagggccaa gaatacagga
     4141 accattctgg aaagtgccca aggtaatgcc cccatcaaga agttccgttc aattgctttg
     4201 taattcattg tttccacatg ttgatcgatc cgatgtgcaa aatacgtatt cgcaaaacgt
     4261 gccaaaatat ctaattggat catcaaactt gctacaggaa ccgcgatggc cgcaatcgct
     4321 tgttctgggt tcatccctag agaaatggag aaagctgttg ctaagatcgt tccagagttg
     4381 gcatcgattt ttgatgcacc accaaatgtc cctaccccta aaacagtcaa ctgcatactt
     4441 ccaccaatga ttaaacctgc ttttaaatca cccattacta acccagcata taatcctgca
     4501 aaaactgggg cgctcattga agaataaatc tgtaattcat ctaaaatttg ataccctgca
     4561 tataatgtta ataataatat ctgccaccat tgaatatcca tgaatcattc ctcctatttg
     4621 attaaaagac tcataaagtc ttctgctttg tcatttggca ccatttgagc gatcaaatga
     4681 acgccaagct gatctaattt cttgaagacc tctacatccg catctacgac attgattgat
     4741 tttgtgatgg aacgtgtttc atctgtttgt gacatattgc cgacatttaa ttctttgatc
     4801 ggtacacctt tttctactag cgctaagata cgatctggtt ttttcgctac gatcagcact
     4861 cgttgagaat catatttacc tgctaaaata tttgcggctg ctttttcaat aggtaaaata
     4921 cttaatttga ctccagctgg cgttgccaac tttaacccac ttttttcgat cgcatttgtt
     4981 gctacctcat cgtctacgac catgatccga ccaatattca actttgtcgt ccaaagattt
     5041 gccacttgtc catgtatcaa acgtccatca attcttactc caataattgc catcatccat
     5101 tctccttttc taactttttt ctattggttc tttgacaaaa tctataactt cactgaatct
     5161 accttctgtt tcaaagataa cgatttcatt ttgtcctttt ctaaataaac cctcgggaac
     5221 aaacagtgaa agaattggtc ctacttccca aaaacgacca atattcgtgc cattcaccca
     5281 aacgatccct ttcccaaact cactaaggtc caaataagta tcaccgattt ctgacagttc
     5341 cgcttcaaag cggtaaaatg ctggttgacc tgcttcccat ttttttgaaa aatcgatgtg
     5401 ttctgtagtc tctaaaggta aacaatactg tgtccattct gtgataaaat gaagatcgct
     5461 catcactcct gtacgtattc cttttctttg cgtatcagct agcagcttgt gtccatagtt
     5521 gacccgtccc atattttcaa ataatagatc aatttgatgg tcttctttct caagtgtcaa
     5581 tggaatggtc tcaccgatct ctgtttggta ttgaatcgct tgcttttctt catcgagaaa
     5641 aacatggacc cgatcacgtc cgtcgatgat ccgtaatttt tcattgaccg catctttttt
     5701 gatcgttgtt cgatacaata ggtacccagt attctgaccc aattcttcca ttgtttgagg
     5761 ataggtcgag tggatcggct tgctgatggt atccagtgtt gcgaaaaagc tgactttttc
     5821 tttcaagttg attccttttt tgacgattgt ttcttttatc catggttcca tttgctgcat
     5881 ttctggaaaa ttttctgcca tcattcgttt cagagcaaaa tatttttccg ttggatttcc
     5941 ctgctcatcc aatggtgcat cgtaatcata ggaagtgact tgcggcaaat caatcgcgcc
     6001 tctggctgaa cagccattca taaacccaaa attggttccg ccatggaaca tatagagatt
     6061 gatactgccg atctgtaatg cttctttgac tgcatccgct aattcttccg gatctctttt
     6121 aatgatcggc tctttccaac gattaaacca gccatcccaa aattccatac acatcaacgg
     6181 ccatttttta ccatgtgctt caaaaaactg ggccatatct gcaaaatttt cttttgcttt
     6241 tgagccaaaa ttccctgtcg taaaaacatc atcgtcaatc aaaatgcctg ctcttaatgt
     6301 ggcttgccaa ggtccatcag aagtgaatag cggtacatcg attccacctt cctccaagta
     6361 tttttttact tggcgcaggt atgctttctc ttcaccatac gaaccgtact cattttctac
     6421 ctgcaccatt aagacgttgc caccatgagt gactagatga ggaacgattt taggtagtag
     6481 cgtttggtaa taccgtttga catacttcat gaagcgagga tccgctgaac gaatacggca
     6541 ctcctttgtc aatagccaag caggcaagcc accaaattcc cattctgcac aaatataagg
     6601 ggctggacgt acgattgcgt ataatccaag ggattgcgct aactctagaa aatgggaaag
     6661 atccaatatt ttcgaaaaat cataggttcc ttcttctttt tcatggagat tccaaggcac
     6721 ataggtttcc acagtattaa atccaagcgc tttcaaattg tgcaatgacc gctcccaatt
     6781 cgtcggatgc actcggaaat aatggatggc accagataaa attttgaaag gttgaccgtc
     6841 taacaaaaac tcttctttta tttcaaatct agtcaagcaa atttcctcct cgtttttaga
     6901 aaccgctttc taattttata atcaacgaat ctttctgacg ttgatttccc ccctaaaata
     6961 atcgactttc acaggatcga tccatcctgt tttttcttgt tgtgcattcg ccatcccaca
     7021 ggccatcgca aacgcaagtg tttcttcaat cgtatagctc tttgatagag catgtgctaa
     7081 gcctgcaata gtcgaatctc ctgaccccac aggattcacc accgcgattt taggaatcgt
     7141 aacctgatag aagtgtctcc ctactttagc gattgctcct tttgcgccta gcgaaagaac
     7201 gatccaattg attccttcaa aaagcggatg atccaataat gattggattt catctaacga
     7261 agtagccgaa gaaattgttt ggtggaccaa ctctcctaat tcttccaaat taggtttgat
     7321 caaaaatggt ttttccttgc cttccaagac ttcaagcaag gtttgaccag aagtatctaa
     7381 caatactcgt gttccttttt tattcgttag ggcaagtaac tctgcataat aacttttaga
     7441 aaatccttga ggcagactac ccgaaatcgt aaagatatca gttgtttcca ttagtcgtgc
     7501 aaaatgctcg gtaaatttat gttgctcttc ttccgtcaca acaggaccgc tttccaatag
     7561 ttctgtttga ttcactccat gtaaaatagc aatcgaatct ctcgtttccc ctttgatagg
     7621 taaaaattga tgcgttatcc ctgattgatc gagttgttct ttgatatatg ctccatgctg
     7681 cccgccgact aatccagtag ctaaaacatc atctgctaat aaatgaagga cacgagcaac
     7741 attcaagcct tttcctccag ccgttttgtt gaccgttttg acccgattga cttggtcgat
     7801 ccgtaattca tccaacgtgt aagcgatgtc aattgaagga ttcatggtaa ctgtcgtgat
     7861 cattttgctg tcaccgaatc ttcttttact ttgtcaaacc aaggggtggc gttttcattt
     7921 atcactttat tcaacgattc gatattttgt cgcccttgtg ttctcaacca ctcaatcgca
     7981 ccttctctac cattttcaac atatgctttg actccatctt tccaagtcgc acgtccgcat
     8041 aacacaccgt tgaatgttga accagattca tgagcaaaga tcaatgtttg ttggaacaat
     8101 gcagtcgaaa cccctgcact caagaagata aatggtaacg gtgttgcatc actttgttcc
     8161 ttgaagaatg ccgctgcttc ttttttacta tacgcaattt caccatcaga ataaccttct
     8221 acatagttca tattgactgg cacttctact ttcaatacat ccactttata tttaggatca
     8281 gaaaattctt tcatcatctc gatcacttta tgcggtttga tcttcgcata ttctaatgaa
     8341 gtgctgtcat tggttttggc atcatacgaa accaattcta gataaaacgg acgcttttca
     8401 cctgcacatt cactgcctaa acgttcgata aatacatgtt tcaaatgatt gatctctggg
     8461 tcttcatcta catcataata aagcaaaaac ttgatcgcat cggctgtttg ttcctttaat
     8521 ctaagcgtag accaatccgc taataaatca ggcagacgtc ctggagttgt cgcatcataa
     8581 cccgtttttt cataggctaa aagtagtcct gcttcctgat gtctggcttt agcagctggc
     8641 aatccatact ctggatctag taaaatagca gaagcataag gcgttaattc tgctgatact
     8701 aattctttga attcagtgat ctgttgcgcc gttggttctt ttcctaacgc tttgatcatc
     8761 tttttcaaag caccgcgttg atcaattgcc aatgcactga tgattccttc ttttgttgat
     8821 aaactcttca tagctgcaat tttttcttca ttcatccttc gtttcccctt tgttcacata
     8881 ttcatggata atgacacctt tgaccactcg attgactgta cctgtaggcg atggtgtatc
     8941 tggtttattc gctactttta tcgacgtatc gagcgcaatg atttgggctg tcatgatcat
     9001 aggtaaggca agatacgcat caggtaggct gatcgaagct tctaacaaaa gacttgtacc
     9061 tgtgaaatcg atgtttcctg gttgggcgat cgcgatgaca ctcgtggtaa tttggtcatc
     9121 ttttatttct tctagaatat ctaagtcata atttcttgta tacggttggt tattaacaaa
     9181 accaataaca agtgtttcgc tatttaaaaa cgattttggt ccatggcgga agcccataga
     9241 agaatcaaag atcgttgcca ctttaccggc tgtcaattcc aatattttca attgtgcttc
     9301 tctcgttgca ccagccaaac ttcctgaacc aagataggcg atacgttcaa atgacttagg
     9361 caataatttt tggatttctt gatctcgtga aattagatct tctccagttc tgatcaattg
     9421 ttgaagataa ctttcttttt tctcaacggc agtttggtcg aatacccata acgcactcaa
     9481 caacatgcac gtaaaacttc ctgtcatggc aaatcctgca tcatttgatt tttctggcat
     9541 cagtaaaaga taatttcgtt catctgtcgc tgcattcttt gctaactgac cttctttagc
     9601 acaagtaatc gtcaaatgaa acaatgtctt gatttgttca ttggcaatct tgactgttgc
     9661 cacactttct ggactatttc cactacgtgc aaatgacacc agtaatgtcg gttcatccgg
     9721 aaatagaaat tcttttggtg aagacacgat atctgtcgta ccaaagctat gaaaaataaa
     9781 gtgatcgtta tcaccgtatt catttaaata aggtactgct gtatccccga catattgtga
     9841 tgtgcctgca ccagtaaaaa tcactcgaac tcttttgcca tttgctgctt tttttatctg
     9901 gtcaaaaaac aatgctaatt tttcttgatt ctgtgctaat atctcagctg tttctttcca
     9961 taattgcggt tgctgtttga tttctcgcgt agttatttcc gcacccaact cttttaattt
    10021 atccttcgat agttcaaaca tgttgttttc cctctcaatc attttcggac gtggcggatc
    10081 ttataatgaa actggtctgc tctcgctaca ctatccgtat attcaataat ttcatttttc
    10141 acattatagg tcgttcggct tagatataat accggggcac catcagcgat acgaagcgga
    10201 tcagcatctt tcttttgaac gatacttgca taaaattctt catctgctaa ttggacttgc
    10261 tgttcataat cctgagaaaa cacatcatac aatggcttac tttcaagtaa tggcagatca
    10321 agaccaataa atagttttgc aggtaaataa gaacgttcca ccatcaatgg ttcgtcatcc
    10381 gctaaacgaa gtcttttcaa tttgaaaact gtttcgccta aattgatgtt tagattcttg
    10441 gcaacctttt tgtttgcttc catttgcttg aattccaaga tgactgtatt aggcacccgt
    10501 cccaattccc tcatctcttc tgtaaaacta tacgctcccg ccagatcaga cgacttatct
    10561 gatagatcag acacatatgt tccttttccg tgtcggcgat aaatataccc ttgcgtctct
    10621 aattcttgca aagcaaggcg aaccgttgtc cgactcactc cataatgctg acaaagttct
    10681 ctttcagata acaatttgtc atgaggtgtc atctgtgttt cgatacgttc tttcaatacg
    10741 tcaactagtt gataatataa tgattttcct ttacttgagc tctccataaa aatacctctt
    10801 ctttactggt tataaccaca acacaagaat acccacatat tcctcatttg tcaaagggtt
    10861 tttggcgtaa aaaagctatt ataacaaggt ttcaaggtaa tttagaactg gtatcaaaag
    10921 atacctcaaa aataccagtt gacaaataaa aaacaaaaaa aagctgaatc ttggcaaaga
    10981 ttcagctaaa tggtctagag aagtaaagaa aaaatattct ttatacatct gtggctattt
    11041 ctactatcga caagcaaatg ggcctaatag actactatgt aaataaaaat ggctttctct
    11101 aaataaagta ggatttttta ttttttagat aacttttctc tcatgccggt ctgatagtcg
    11161 aaattttgtt gtacattttt aaaaataaca aaaatttttt atgataattc tgtctttttc
    11221 tagtttcctt atggcgtaag t
//
LOCUS       DASJSZ010000086        11124 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.090, whole genome shotgun sequence.
ACCESSION   DASJSZ010000086 DASJSZ010000000
VERSION     DASJSZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.090"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(48..1683)
                     /locus_tag="U4B03_002291"
                     /pseudo
     CDS             complement(48..1683)
                     /locus_tag="U4B03_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2122..2991)
                     /locus_tag="U4B03_002292"
     CDS             complement(2122..2991)
                     /locus_tag="U4B03_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2069111.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(3011..4132)
                     /locus_tag="U4B03_002293"
     CDS             complement(3011..4132)
                     /locus_tag="U4B03_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN2069112.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4134..4529)
                     /locus_tag="U4B03_002294"
     CDS             complement(4134..4529)
                     /locus_tag="U4B03_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2069113.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4548..5354)
                     /locus_tag="U4B03_002295"
     CDS             complement(4548..5354)
                     /locus_tag="U4B03_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2069114.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5347..6135)
                     /locus_tag="U4B03_002296"
     CDS             complement(5347..6135)
                     /locus_tag="U4B03_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2069115.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6149..6625)
                     /locus_tag="U4B03_002297"
     CDS             complement(6149..6625)
                     /locus_tag="U4B03_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2069116.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(6697..9354)
                     /locus_tag="U4B03_002298"
     CDS             complement(6697..9354)
                     /locus_tag="U4B03_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2069117.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNIRKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(9373..9777)
                     /locus_tag="U4B03_002299"
     CDS             complement(9373..9777)
                     /locus_tag="U4B03_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEN2069118.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(10009..10266)
                     /locus_tag="U4B03_002300"
     CDS             complement(10009..10266)
                     /locus_tag="U4B03_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEN2069119.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(10391..10723)
                     /locus_tag="U4B03_002301"
     CDS             complement(10391..10723)
                     /locus_tag="U4B03_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069120.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         3562 a         2191 c         1590 g         3781 t
ORIGIN      
        1 cttacttttt cttattctct agtaagctaa tattgaaaaa aatgtaatca gatcattatt
       61 tttatatgtt gtaacttcct gataagaaaa ctaattaaaa gaaagaggta actacataaa
      121 ctaattaaaa aagctaactt catagttgga ggataaatgg aagaaacttc aatgattgtt
      181 tctccttgtg ttccttcaat cgacactagt cctattatca atctctcttt tttgtccatt
      241 tacttttgcg actgttcccc aataatcaaa tacaggaagc gttatttttt gtcctttaga
      301 ttcatgtact gtatagacta ttttcgaatc catcacttga taaggcatag tatgccattc
      361 cttgccaatt cttacttcat gattctgcaa atgttctttg tattgtaatg cttccgtggg
      421 tgcatagtca taaagtcctc cgggaaggag tgtcgtgtaa cgaataagat tttcttgatt
      481 cattaccaag tgatctctct gagtgacaac tttatgttca tagtttgctt ttgttgcagc
      541 agatacagcc aacagtaata aaacaattgc cgttatatta actacttttc tttgcttagc
      601 tgatgtgctc ttcatgaaaa cgaacgataa aatcaacgag ccaaacagta cttgaaagcc
      661 taagattcgc caaggaaatt gaatcatttg aatgggtgta ttttgtaata aagaccatgg
      721 aaaaaatttg gtggataaga gccaagagac taccgcacta aggtaaatca ctttttcttt
      781 gcgattaaat cctttaaaaa agaagagtgg acagatcaaa gaaataatac caatgattcc
      841 taccgtaaag gttgtcaggt cattattcat cgcaccaatc aaactatccg agacatttaa
      901 tgcttggtgt tgtaaggtcg ttttatctgg ctcattaatc gattgaacca tgatttgtcg
      961 gaacatcgga taccaaaagt atgctgttaa aagaatcgtc attcctactg cctttgacaa
     1021 ctccacaatc aaccggatgc tttctttttt tcttgtttca cttactacaa agccgcctaa
     1081 gactagtaag ccaacggcta ttacactcat aaaagtggaa agaacatgtg tataaatgag
     1141 taagctcata ctgactgcta atggaattgc acatggcttt ttctcataaa tcagtgcgta
     1201 agcataatac ataactatcg gtataaccgt aagtgcaatc gcttcagcaa tagcagaacg
     1261 atgataaata tcaatcgtac ggtacccata aaatgtatat aaacagctaa atataaaagc
     1321 cgcatcgatt ctttttaaaa attttagtcc ataatgaaac atcagcatga tagtacaaac
     1381 attcaatagc caaacatata agatataact aacgattaaa ttattactta tccaataaaa
     1441 aatgactgcc ggaaaaaagg tcaagtaggg ataaaaataa ttcactccgc tcccataatg
     1501 attaaacgtc gtaaaattga ttggaccaga aaaaattgaa gataaccctt tgactctagc
     1561 aaaatggaag gcaatatccc ccgatgtaga aatagtatga acatttctaa aataattgat
     1621 tgaataaagt ccagcaataa ttgctaggag aatggacaat attatttttt tctttacttg
     1681 cattacttaa ttatatactc ctttcatgga ttgctttatc tagtataact taaattttaa
     1741 ataaatcgtt atttattttt actctcactt aaaaaagcac taaaattata agagagggag
     1801 gtaatggtct gaacaccaag aattgttcca acatcgaatt gcttaactaa tgagttcaaa
     1861 gcttaattat gtggctctta tatttaccga gcgtggattg cgatctcttt agcgaaacaa
     1921 acatctttca taaaaaaaac agccatatcc gctttcttgg atatgactat ttactactac
     1981 tttactatct ttcattcttc ttgtctcact tatgataagg cgctgggtat catgagtgaa
     2041 tgatattgct gaatacaaat agtccgacca cattaagttg atcggacttc ataagcattt
     2101 tattgactaa tacttttttg cttaagaatt gattctaaca tcttttgcta aagttaagca
     2161 tcctatagtt cctgcattat ctgctaactt aggtgtgaca atgtattctt cgatatttgg
     2221 cgtatcgaca tagccgttaa ttagttctaa aaaacgtctt tgtactttct ttttcagatg
     2281 ttcttgttgc atgacaccac caccaaaaat tataatatct ggtgaaagca tcagggtcgt
     2341 attacaggca cattgtgcaa tataatccgc ctcgatttcc cagaacggat cttctataga
     2401 aagatcttgt cccttggttc ccgtacgcat ctcgatagcg ggtccagatg ccaacccttc
     2461 gagacaatca tgatgaaaag gacaagtacc agagaaagta tctcctgaat gtctcttaac
     2521 taacgtatgc cccatttctg gatggctaaa cccttcaaca aaaatcccat cttgtatagc
     2581 tcctccacca attccagttc caacagtgta ataaacaaca ctggataaac cttttccatg
     2641 acctgaaaca tactccccgt aacaagcagc attcacatcg gttgtccagg caattggaat
     2701 ttcgaaatgc ttattcaacg ttccaacaaa atcaaaattt ttccaggcag tttttggagt
     2761 agaggtgata tgtccatagg tctttgattc acgatggata tcaatcggtc caaatgaccc
     2821 tactccaatg ctttctaatt gttctttata gtgattaaag aaatcaatta ccaaactcat
     2881 tgtttcattc ggtgtagttg taggaaaact cactctttct aaaaccttta aatcatcatt
     2941 gccaatggca caaacaaatt tagtcccacc tgcttctatt gagccataca tttcttactc
     3001 caccgtcctt ttatctagga attcgttctt ccctgtctaa tagtttttcg aaaaactcat
     3061 gtggttcctt ttggtaccaa taggcaacag aagaaatatc gtcttgacgc tcataaagct
     3121 tgatgtcatc atttcctagg gtttggaaag taacacgtaa attttctttg aaactaatgg
     3181 ggtcgggtaa atgccagcgg tacaacccat gcatcggtaa agcatcatcg ccaaaaccat
     3241 gaactggatt tggattaggt tttccagcac taaaataatc ccgggtgtga tctcttttag
     3301 attgaaaagg atagcccata aatagcgtat taaaattttg agcatatgcc ctttctccaa
     3361 agttcttttt atggaaagcc caagcgccgc caaaataatc ttctgctcct gtagatgtta
     3421 ttgtagggaa atcttcatct ccatctatat aaaacttaaa ttctccttcc ccccaccagt
     3481 atctttccaa tgcgcacaaa gcaaagtagg ttcctacata atacccagtg cctttgattc
     3541 catcaataag ggtgtaatct tttccttttg tcgtgatggg ttcacggttc catttagcat
     3601 gaaaatacat cgcatcatcc ggtacttcag tcaatgcata attaaatgta taaaaaatgt
     3661 gcttaataaa ctcaggatgt tcattcgtta aagtaatctt agcttttttt cgaaatggca
     3721 tctcaaaata gcaattcatc cctgatgttg gattcactac gattggtaag gaattcactt
     3781 cgcatcttgt cccaaaccca ttacaaaaaa aatctcctaa tggactttct acagaaggag
     3841 tggattcatc atcccaataa atacgcaaaa ttaaatcacg taagacgaaa ctcccagcga
     3901 ttgttttgtc tgtcactgtc atccaaaaat gacgtaaaat tccagggcct tccatttctg
     3961 ctaaagtaat tgtctctcca gcatttatcg gaacagaagg tgaccctttt ctcccaattc
     4021 ctagatgact cgctgtcatt gccgctttcc ctttttcacc atttttattt tctggtgaaa
     4081 tagttctact ttcctctccc ttgtggagcc atatattatt taaacaattc attttattcg
     4141 tcctctatcc tttcttcact tttttgatta ttcatataga tgatttgttg ttgtgcctcc
     4201 gcaataattg ctccaatatc taaattatca tttcctaact gcaaagcgat tgacattgcc
     4261 aagggaagat tcattcctgc aattacgtgc actctgtcag aaatataggt aaaaaacttt
     4321 tgatttacac ttcctccagc catgtcagtt aaaacaatta tttcgtctgt agtatccact
     4381 aactctttaa tttgttcatc tatatcatca atttcttcga catatgcaca taagctacta
     4441 atttgattat gcatacctgt taaaaactct agtgtctctt taaaaccctc agctagtatt
     4501 gaatggctag caatgatcaa tcttctcata attcaaatcc ttttccttta cgctaatatt
     4561 ccaaatgcag ccataaccat cgcaaaaaca ataacgagta gaatcaaaat ggtcatactg
     4621 atttttttct tactgagtag ataatatgct gcagcagtaa taattactgg aattaatgtt
     4681 ggtaaaattt tgtccaacat tggttggatt ttcatactaa cttcaccaaa agtgaattta
     4741 agtggcgttg aaacactgat aactgaagca attaatgatc caactactgt tagccccaat
     4801 actgaagcag cttcagttaa ataattaatc ttttcaccgt attcagtaac aatcttcatt
     4861 ccctgcttat aaccaaattc aaagcctctc attcggaaaa agaagattac catgattaaa
     4921 atgaaccata aaaagattcc tacaggatta ttttctaaag ccatataagc cgcaattgat
     4981 cccatgatgg ttgggatcat cgtcatcaaa agagagtccc caataccagc aattggaccc
     5041 atcgtaccaa ctttaaagtc attaatcgca tcttttgctt ctaatccttg cttttcctcc
     5101 attgcaacac aagcacctaa aataatggct gccagccaag gctgtgtatt gtaatactga
     5161 aagtgattat ttaaagcttc gatgtattca tcatcatctt tgtataattt tcttaaaata
     5221 ggtgacaatg cgtaagtcac agatgcacct tgctgtgttt gataattaaa ggtacaaact
     5281 gacatcaacc aacgatagtt cgctttccta agatcctttt tcgttacttg atatttttcg
     5341 ctcattttat tcatcctccc caataaaccc aacatcagaa gctcccgcag aagcagtgat
     5401 ttgtgcatct ttttcagctc tatgatacac attaaatgct aaagcgaatc ctatgatcgc
     5461 cgctccaata ataggtacct tcaaaaaagc ggctaagaaa aatccaacaa gtaaataact
     5521 aatgtatcga cctgtaggca taaagcgtaa caacatcgca ataccaacag caggcaacat
     5581 ttttccggct aatgttagcc catccataaa ccaattaggt aaaacatcca aaacgtaatt
     5641 aacagcagac tgtcctaaag taacggcaat aaatactgga attgctgcag ataaaccaaa
     5701 gaaaattggc cccaaccaca aaacactatt cattttctta aattctcgtt tattcgcata
     5761 ttcctgtgat ttgcgaacaa caaaaccatt taagattttg acaattacat ctagttgcac
     5821 ccctaacata cctacaggta gtcctattgc taatccggct tctactccaa gctggctacc
     5881 aaatgccgtt gcaataattg cgcacgttcc atagtccggt acagatgagc caccaaaacc
     5941 ggcaactcct aatgacatca attgaactgt accgccaact atcaaacctg tttttatatc
     6001 acccatcaaa attcctgtga ataatcccca aaaaactgcg taatttacaa agatcatcgg
     6061 aatcccataa taatcaacat gtttaataaa tgctaatata gtcagcataa taatttgaaa
     6121 aatagtaaaa tgcattatcc ttactccttt atcctaattt tgattgaatt tcctgtgcct
     6181 tatccgccgg aacaaattgt gtaacaattt ctacaccaga atccaacaat tttttatacg
     6241 tgctgatatt ttcatctgat gcataggctc tagaagatag ctgtaatgca ttatctttaa
     6301 caacagtacc gccaacaatt agtcggtcaa ttctaatacc gttatctact aaatgaagga
     6361 ttgtttccgg ctctttcacg atgacaaata ctttttcctg atcgtatttc ccctttttaa
     6421 aattcgcaat cgctgtttct tcagtgataa ttgatacagc cattccagca ggtctagcta
     6481 aacgcatact tgatttttgg atgtcgttat ttgcgacaga atcatcgatc accatcactc
     6541 tgtttgcttt agacattggt gcccattgcg tgacaataat cccatgcaat aaacggttat
     6601 caactctcga cataacaata ctcataaata cttcctcctt tatctttcat tttcttacaa
     6661 gcaattacca tgccaaaaca aaaagaactt gataaatcaa gctcttttta cataatcata
     6721 aacgtattta acctcatgaa tcgggatgtc gacactataa taatgttcaa cgacactgaa
     6781 agattgctta aaatgtgtct gaaatttttg aaattcctca ctcgtttcat tgaaataaat
     6841 ttcatcttcc aaagtatttt gttttactaa tcgttcaatt aagcaactaa tgtgaacata
     6901 taatccgaaa cacgtattat tatcaaatga aattcctaga tcattttgta agatgtatat
     6961 agcatctgaa acccgttcta ataatttagt cggatttaat atggtcaatt gctccattaa
     7021 attcgttaga ctaaaattct tcagaatatt attgttcaaa actttcagtt cattatcatt
     7081 aaagtgtcta ttcaattgat tggagaattt tcttatattc attcctaaaa tcatatcttc
     7141 aattgaaaag aattctacat cttctagatg tggatttagt gttccaacaa taaaaagaat
     7201 attgttctct ttacgaagct ccgataaata gttttcattc actaaagagt aatagtcaca
     7261 cgtttctatg acaagatcag tatcccttgg aaagctatct tcaagtatcc tttttaattt
     7321 aaacgaagtt cctaatccgc ttgcacaaga acagacaatc atatccttct taatatttga
     7381 tgattcataa atataatcca cattttgatt ttcttttact tgttcaagaa tctgtttaag
     7441 cggtaaatct aataatatct gatttgcaac atcaatagcc attcttgttg aaacattgtt
     7501 tagtagtccg aatttttttt gagcgtctag ttccaaattt agataaattt tctccagtga
     7561 acccatatca acaagtaaaa taatttcttc atatccggta cactcttgta agtatctatt
     7621 aattttgtct gcaatctcac tacttgaagt cattagtggc atatcaatgg cgtcgtatat
     7681 atatctctca acgatcctat tagcagtact tgctatacag gttgccgtta gatctccatg
     7741 agccaaaatc agtgctaaac gtttttgctg aaattcttta ctacacatta acttaaataa
     7801 aataaccaaa acattttttt gaatttttgt taaataaata tcccctattt gaataaaacg
     7861 attgactaat tgaacttctc gactacacca actattaata tcattgatga aggaatataa
     7921 tttatcgtcc gatgttaatt ggatttgata aatccaagaa ttcaaaaaga tagacgttat
     7981 ttgaagataa taatagtcaa attctatttg aaaatctctc tctatttcct taacatactg
     8041 tctaaatcga atctcttgat aggtgggata tgttaaagct gaattatttt ggatatcaac
     8101 attcttttgt ctaaaatatc tatcatattt ctcaaaaagc tgctgcttat cagtgtcttt
     8161 cttccacgtt tgataaagat actctataag ttcgttgata ttaaaaactt cgtctacttt
     8221 ttccaaatca tccatttgaa tatattttaa ttcattctgt cttttcataa ccttcatgcc
     8281 gttgaaattc acccattgtg gtaaatgata catctgaata taaagttctt ccaaatcatc
     8341 taagaaataa aaggcattgg cacaggtcaa ttgaatgata tttttcaaat tgccaacatt
     8401 ttcactaaag cgatagttta ataaaaaatc taataattgt tgagatatca gtattttctt
     8461 tgctagtctt tcttcttctg atttaaaaaa tatcttgatt aactgttttc tttcataaag
     8521 agaacgactg tctaatgttg gaagtgttag ctttacaggt attcttctca acaaagttga
     8581 taatagttta tcctcaggtg gttctgatgt tgcaaatata atccagcaac ttgatgtgta
     8641 ccagttttca ttatcaccca tccgatgaaa tttcctcgta tccataaact gaaaaagttt
     8701 ctcctgacat tcattgctca atgcatgaat ttcatctaaa aataagactc cattattagc
     8761 taattctgct aaaccctttc ttccatcttc ggctcctgta tatgcacctt ttacatgccc
     8821 aaaaaaattg gacaagaaaa gttctggatt attggcatat tcactacaat taactgaaat
     8881 gaaagatgcc ttctcttcta tataattatt ggccaacaaa tccttataca cataatttgc
     8941 taaatagctt tttccagttc cagtctcgcc atgaagtaaa atgggtaagc catttggcgg
     9001 atagtagatt gctgctttaa tttgagcaat tacatttcgc agacttcctt gacttccgat
     9061 aaccctttct attccaaccc taaaagagct atcataccat ttttccgctt cctctgaaga
     9121 gctaaaaatt tgttgatttg gaactttctc cggtaaagaa aagaagatta ctggacgagt
     9181 attgattttt ccaaattctc catcttttac gaattcattc aaatattgag agacaatatt
     9241 acgactaaca tttaattttt cagatatttt ctgtgctgta ttgttccccc atttattttt
     9301 aatctcagag ttctctatga cataattgac aatgcgttct tttaattctt ccatcacagc
     9361 acctcctcga cactattcga attttgccga cagtattttt ccataaatat gacttgctaa
     9421 aaaggctttg tttagatcat tcaattggaa cgttgcttgt tttgcttgat ccgctaagac
     9481 gttgaaaaga tactctggcg aaatcgcata ccccaaaata tcaacgattg ttgcaatcag
     9541 atcttctttt gattcttcat ctgaaagtaa ttcacgatac aacatcgttt gtttgcttac
     9601 tgtgtcatat tcctctaagg attcatctgc taaaagaacc acttgttcca acaaacggtc
     9661 ggataaatat ttatagaaaa tcagccccaa taaataattt ttatattctg acgcatccat
     9721 tttactacgg agattatctg ccgcactaaa taacttctga tttaattctg cacccaaaaa
     9781 gatacactcc tatctcttat ttaaagttgc tatataccct cttattttag catactcagt
     9841 tttagaaaaa gcaaaaaaat aaagaaactt attcttgata acaagctacg tgacgtatat
     9901 ccctaacaca aaaatagcca tatcctttgg tttggatacg gctatttgat attactcatt
     9961 caattatcaa atcacagtag gtgtgaatct catcgggatc ggcaatatct acttctgatt
    10021 aggatcaaag gtgattttct caataaaggt gtgatacaaa gctttaactt gcttcttctc
    10081 actctggata agaaagcaat ccaaactagt caatatctgt tggacatctt tgacttgaat
    10141 cggttcccct tgttgttgaa agatccctaa gatttcattc tctcgaattt gtaactgtct
    10201 tcgtttttca gtcaattcat ttaaacgatc ttttaacatc ggaaacaatt cgggactatc
    10261 ttccaaagca gcttcctgtt tttgctctaa tagttagatt ttgcgtacca ttttcttctt
    10321 ttattttcct tcgattgctg attttaatat agtctaaagg aaatttatat ttattgtatt
    10381 ctttataact tcaagagaac ttcgtttgag tattactgta tttttttaat tccataagaa
    10441 aattagttat tacctgccac ctaatcagca aatctgaacc tgagtcaata taattaatat
    10501 tattttccat ctccaacatt gtaagtactg ttggtgttaa tatagggact ttgtacaatt
    10561 gtattttttt gattttatca tctgcacaaa ggagattttt taaatctaat ccttctttta
    10621 tttcaaaagt tgaatttttt aaatctataa gagtaaaacc aattccttca tactgaattt
    10681 ctgaaaatcg aatttttctc ccagttgata gatcaagggg cagaaaacct tttattacat
    10741 tgtgagtgtc accatcattt acaatgtcat taggaatttt gattttagaa ttctgtggag
    10801 cacctattgg aagataataa ttcattataa ttttgtagtc ttcatgagga aataaagtac
    10861 ttttaggatt gctaagatat tctcgtaccg aatctaagaa tggcttatcc attgaatctt
    10921 catccccatt taaactaaaa aacattccta caattgcttt tgcaactcta agtgaattca
    10981 ttttctttat tgaatgtgtt aaatcagact taccattctt taaacttgta ttgttataaa
    11041 ggaaattttt attgtcttct gcaaatctaa tatattcctc aacatactca ctaccaaaaa
    11101 aactattaca tttactacat aacg
//
LOCUS       DASJSZ010000087        10848 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.091, whole genome shotgun sequence.
ACCESSION   DASJSZ010000087 DASJSZ010000000
VERSION     DASJSZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.091"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..476)
                     /locus_tag="U4B03_002302"
     CDS             complement(99..476)
                     /locus_tag="U4B03_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069121.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(776..1657)
                     /gene="galU"
                     /locus_tag="U4B03_002303"
     CDS             complement(776..1657)
                     /gene="galU"
                     /locus_tag="U4B03_002303"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN2069122.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1700..2719)
                     /locus_tag="U4B03_002304"
     CDS             complement(1700..2719)
                     /locus_tag="U4B03_002304"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN2069123.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3352..4356)
                     /locus_tag="U4B03_002305"
     CDS             complement(3352..4356)
                     /locus_tag="U4B03_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2069124.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4369..5418)
                     /locus_tag="U4B03_002306"
     CDS             complement(4369..5418)
                     /locus_tag="U4B03_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2069125.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5435..6256)
                     /locus_tag="U4B03_002307"
     CDS             complement(5435..6256)
                     /locus_tag="U4B03_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2069126.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6243..7022)
                     /locus_tag="U4B03_002308"
     CDS             complement(6243..7022)
                     /locus_tag="U4B03_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2069127.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7039..7509)
                     /locus_tag="U4B03_002309"
     CDS             complement(7039..7509)
                     /locus_tag="U4B03_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2069128.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7521..7940)
                     /locus_tag="U4B03_002310"
     CDS             complement(7521..7940)
                     /locus_tag="U4B03_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEN2069129.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8021..10798)
                     /locus_tag="U4B03_002311"
     CDS             complement(8021..10798)
                     /locus_tag="U4B03_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2069130.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
BASE COUNT         3103 a         2317 c         1783 g         3645 t
ORIGIN      
        1 atataagtga aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat
       61 ccattatttt atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt
      121 ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct
      181 atccgagact ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat
      241 aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc
      301 ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga
      361 aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc
      421 atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg
      481 aacacacacc tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg
      541 gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa
      601 accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc
      661 cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa
      721 gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac
      781 tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt
      841 ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt
      901 cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa
      961 gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa
     1021 atattttagg agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca
     1081 tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag
     1141 gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg
     1201 aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca
     1261 tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa
     1321 gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag
     1381 tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca
     1441 attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca
     1501 agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat
     1561 ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta
     1621 gcccagcggc aggaatgact gcttttttga ccttcacttg ctgttcctcc taaacgacga
     1681 ttaactattg atcatctatt taacgaagag aaaattcatt ctccattttt ccgtcacgta
     1741 acatgatttc ttttgctgct tttttgatat cttctccatt atacaaaaca ttatagattg
     1801 tttgagtaat tggcatctca acacctagct gttgagctag ctctactgct gcttttgtcg
     1861 ttgccacacc ctcaacgacc attcccatgt tttctagaac ttcttccagc ttatgtccct
     1921 gtcctaacaa gttaccggca cgccaattgc gagaatgaac acttgtacaa gtaacgatca
     1981 agtcccccac accactcaaa ccgataaatg tcagaggatt ggcacccatc gcgacaccaa
     2041 gacggctgat ttctgccaat cctcgagtca tgattgctgc ttttgcatca tctccgaatc
     2101 ctaatccatg gatagcgccg gcaccaattg caatgatatt tttcagtgct gcaccagttt
     2161 ctacgccaat cacatcatta tttgtataaa tcctgaaata atcattcatg aacagttttt
     2221 gtacataggc tgctgctttt tgatcaatac ttgcagctgt aattgttgtg atatcatgta
     2281 ctgccacttc ctcagcatgg ctaggaccag ataatacaac aattgcctgt cttttttctt
     2341 ctggaatttc ttcagccaaa acttctgaga ttcgtttatg cgtatcttgt tctagtcctt
     2401 tacttgcatg tataatgatt ggcttagtat tcattttacc aataagttcc tgtgcgactg
     2461 aacgaatcgc ttttgttgga acaacaaata aaatagcatc tgcgttatcc actgcttctg
     2521 ccaattcttt atatccgata atcgattctg ggatcgtcaa atcaggtaaa aaatgctgat
     2581 ttgtatgata cttattgatt tcatcaatct gttcaggtac atttccccaa atacggacat
     2641 catgcccgtt ttctgccaat gtttgtgcta aagcagtgcc ccaagatcca ggaccaagaa
     2701 ctgcgatttt ttgtttcatg gatattcctc caaataaata attgataatt tccattatag
     2761 tatacatttc ttaaagttgg gactaatttc cattgttttt aagtgtttgt ttacttcttt
     2821 tatcgttttt acttgaaata atcaatgata tgttacaata gtctacattt tatttttaca
     2881 ctcgttatgc tttgcttgtt cctaagagga agcagttttg acaatgttac taccagcatt
     2941 actattagga ggttaggaaa cttctcctag tgagtattga tttaaaatac ataagagaca
     3001 gattgaacat ttctaaatct gtcaggtcct gcaacggaca gattgtctct gttgacggtt
     3061 gacataggaa cttttgtttt agtcaacatt ctggatacaa aacgctatgt aaactggcgc
     3121 aactcgttgt acttggactt ttactacgga tccatgcaac aactaaatag tactattgca
     3181 taacaaaaaa tggttggcac ccacatgtcc cattaaacag attataagtt gaactgtaga
     3241 taatgcaaaa aatgtgtttt ataacgtaat tgatgccaat attttaaaaa aataaaggga
     3301 gcagttatcg ctgctctctt tattttttat attcttttta tccagtctat cctaataatc
     3361 aaactgacga tagtagcggc gtatctctaa tggatggcag ttcaactgct ccacatgagc
     3421 atcgatgcgt tgtgtcacta catgcatcaa taagaaagac aaaacacgtc cgcgatattt
     3481 ttcactgata ccttctactg gatagtcttt tgtgtctatc aatgtataat tgctgcaaat
     3541 ttttggcaaa agtttagcca cacgttcaga taacactctc tgttcatctt cgcctaaaaa
     3601 taatgtgact ggggttgttt cttctacgat ctctaacgtc ccgtgaagaa actcggcagc
     3661 atggatagat ttcgatggaa gccagctttg ttcttcccag taacacatgg catatgaata
     3721 aacagctccc cattgattcc ctgctccaat aaaataatgc atactatcac gtcgatgttt
     3781 ttctgcaaat gataagccaa atgcatctgc ttttttctct gcttccacta accctgttgg
     3841 taaataacgt tcgagctgtt catagtattc tgaataagct tcgaattctc cattcaaata
     3901 catcaatcga tctgctgcca taaaaaattt gatttgttct gttccaggag ctggataagt
     3961 gaccacaaaa tcgcatagct gagacaactt actgttcgct ttatcgataa atccaatcag
     4021 tgtcgctcct actttcttga tttgttctac tgctttcact acttcttgag ttgtaccggt
     4081 aacagaggac aaaataacga tagaatcttt tgttaatctt ttgtttccag tagtgtaata
     4141 ttctgcagca tgctgtacaa agacaggcaa gttgcttttc ccattcatat aagtgacagc
     4201 ttgcatcgag gaagcatacg ttccaccgat tcctagataa tagacagcgt caaatttttc
     4261 cgcataaagt ttatcgatga tttcttctac ttgcggacgt aaagctagtg cgccttttat
     4321 tgctttgatt tgttcttctt cattgaattt cagcatgatt tccacctctt ataatttact
     4381 tttcaagact gtatcaaagt cttcaaaaat ctttttgttc aagtcaatac ctttagctac
     4441 tgcggtttta taagcgaata gttgaaacgg aacgacaagc gctaatacag acagatattc
     4501 atcacattct attgctaatc caagtgtttt ctcgtttctt gctttttttg tggtaatggt
     4561 gagagtctct ccaacgtacc gactcatata tctttgcaac ttttgcgaac gttccttatt
     4621 tacactttcc ccttcaataa agaatagcaa atgcgaagca tctgcctcca ggtatgggcc
     4681 gtgcatataa gcttctaact caaatccttg tgacggaacg cggattgttt cagtcaattt
     4741 cgtctctgct tctttagctg ttccccagtt tggaccatat ccaatggtga cgaaacgctg
     4801 tgctaaacga aataaacttt gatatccatc aaaaaattct tccgtttttt gaataatcgc
     4861 tggtaaatgg ttgattattt tgcgcaactg ttgcatgtag tcttgttcta tttttttgct
     4921 gattttattt ttacttattc caattcctat ccctaagagt agtaactgta aaacagtcgc
     4981 agaatagcct tttgtaacga agcctactga ttctaccccg gcacctaaat caataatttg
     5041 tgcagcttct aaagcaagtg gactttgcac gtcatttgtg atagcaacaa caggtaaccc
     5101 ttgtttttgt atcattttta ctgcttcaac agtagaagca cttttcccgc tttgtgacac
     5161 agcaatcaca gtatcgattg aaggagaaag ttttccataa ttgaaaaaat aaaaaggttc
     5221 ttgaatatcg acagtaagat caccataaga ttccaatgct ggcttcgctg ctaaacaagc
     5281 gttataagat gaacccgttg ctaagatcag taaattcact gtttttttca tatgttctct
     5341 tgtcgaaaaa tcattctttt tcaagatcgt acttagtact tccttttctt catagatata
     5401 atcttgcatc gtaaacattt tctctgctcc ttttttaaaa aatccctatc cacgaaccaa
     5461 aaattccaaa gaaagctagt ccgatcaaga tcgttaatgg tttcacttct ttcttcaata
     5521 gataaaacac tatgccaaat gctccaagac ataagatatt tggaacgata tcatcaaaaa
     5581 tactttgaac agtcacagca ttttctcctg aaccgatttt taaaggaata ttgatggtga
     5641 tcatcgtggc agtcattgct ccaacgacca tcagtccaat aatagacgcg cctaatgtca
     5701 aactttccat cataccgttt gcctggatct ttttcaaaaa acccgttcct aagcgatatc
     5761 cccaacctgt tgctagatag cgaaacagaa tgtgcggaat attgaaaatc aaaatgaaca
     5821 agatagggcc taaaatattt ccttgcatgg ctaaagaggt acccacacct gtggcaatca
     5881 atcgcaaagt tccccaaaag aaagaatctc ctaaccctgc aagaggtccc atcaaagatg
     5941 ttttgatact atcgatcgca gtcacatcaa attcaggatc atccttattt tcttcctcca
     6001 ttgcagccgt gattcctaaa atcagtgtaa cgatatgtgg ggtcgtgtta aaaaactcta
     6061 gatgccgttt caaagctgct gccatttctt ctttcgactg atataacttt ttcaaaatcg
     6121 gaatcattgc ataaacataa gccatattca tctgacgttc ataattccaa gaaaactcca
     6181 tttgaaaaga acgccaaaat acgtgattta gctctttttt cgttattttg ctgtttttga
     6241 tctcagaatt cttcatcttc ttctacctcc cctgcagttg tttgaagtgg tagttgacgg
     6301 acattcataa tattgactac cacaactgct aaaatcgcac caaaaatagc tactccggtc
     6361 acaggaatct ctaagtaaga agctatagca aaacctaaga aaaaatatgg tgcaacttgt
     6421 ttgttgatca gcaaacgagc aagcatcgca aaaccaagag ctggaatcaa ccctgtagct
     6481 actgacaacc ccttttgaac gaagtcagga atcatattta atagatttcc gatcgtattg
     6541 cttcccacca tataagaaac cattaccaca acaccaagca tcaaggaaag accgataccg
     6601 gcaagcaagt gcatatgctc gactccttta taatttcctt cttctgcata attgtctgct
     6661 ttatggttca taattggaat caaaagccct aagtaaatat ttttcaatat caatgtcaac
     6721 gtagcaatcg gcaaacctag taaaagcgcg gtttctgtac cggctccagc tgtaatagca
     6781 aaagccgtcc ctaaaatccc accggtcaca acatcaggtg gaatcgaagc acctactgag
     6841 aacgatccga taaatgctaa ctctaaagtt gcgcccataa tgatcccagt ttttacatca
     6901 cctaacacga ttcctgtaaa cagccccgtc acaatcggac gagaaagcaa cgatgtccct
     6961 agtgcatatt ctgattgtgc gacaaatgca acaattccta ataagactgc ttgtacaatc
     7021 attgaggttc ttcctttctt acaaaacttc tcttacatcc acttttttat ctgctggtac
     7081 ttgacggatt tctacttcta tttgtttgtc aattaatcgt ttcaagcgtt cctcctcttc
     7141 tggtaaaata tttaccgctt ttccgatgct gcgagtacca ggttttgcct tgatccctcc
     7201 taagttgatg gttttgattt ctggatacgc ttctgccagc ttatatgcat cctcgatcga
     7261 ttccactacg atgaataact tatatttatc tgtcaccccg cttttcaacg cggcaatcga
     7321 gtcatcaatt gatttgatca ctagtttgac tccttgtggc ttggctaatt tgatcgttgt
     7381 ctttcgtatc tcattcgtag gtacatcatc attagcaatc aaaatacagt ctgcgcctat
     7441 tgtttgtgtc catgaaaaag ctacttgtcc gtgtaataac cgatggtcga ctcttgtcaa
     7501 tacaatcatt tttcttcctc ctaaaattcg tcttcttcta ttttctgatt gagccgctcg
     7561 ttacaaaact ggatcatttt ttttgaatca gttagcacct gctggatcaa aacagttgtc
     7621 tctacttcag cgtccaaccg agatactagc tcaatcaata gtggcaaatt caatccggca
     7681 acgagataga catgatcccg attgatatat tgtaagaatt cgttgttgac acttccgcca
     7741 aaaagatccg tcagaacaat gatttcttta tctttgtacc tcataaaaag ctgccttact
     7801 gtttccgcta aatcaaaatt ctcatcagta tagcaatcga atacagtaat agaatgattt
     7861 ttcccataga tcagctctaa tgaatctaat atccctgaag caaattttcc atgggaagct
     7921 aagataattt ctctatccac tttttcatct cctcttttca cttacacata ctaaaaaagc
     7981 aagttgcatg ccaaaattag tggcaacaac ttgcttatgt tcaaaattct tcttctatcg
     8041 agagaaactc tgtatcttca ctcaaaatat catagatata ggcaatctca gaattgggga
     8101 tcatgacact ataatgctct tcaatgacac taaacgcaga tttaatagca gataaccctt
     8161 ctttttgaca ctgcttccat ttctctaatc cttgataggt ttcgattggc atttgtcgga
     8221 tcaatcgttc aatgagacag ctcacatgga catacaacgc taattttttg ggattgctga
     8281 tgcttcgatt caactgcacc tctaattctc tcataaaatg atcgatttct cccatcacct
     8341 tctctgtatc tagaatagtc acggaatcca gtactttttc caatgaaaaa ttacgaatga
     8401 tgcgctgatt gaatatttcc ttctctgaag cattcatagt agtgtccaac cattcagcta
     8461 gtacagacat cttttccccc gagatgaggt cttctaatga aagatagtcg atattggtga
     8521 tatcaggatc tgtggtgcca atcattccta cgacttcgta tatggaaaat aatgggtcac
     8581 tgtttttctt atcttttaat tgctgatatt catatgggat gattttcaac tgacaagccg
     8641 taggtaagct tttttctagc agttcactta agtggaccgc cgtaccgatt ccagtgaagc
     8701 aagtagtaat gagtgctttg ggtttgtttt cctctggata gagcagtcgc cattctggct
     8761 ggatctgtcg aacagtctct tctgctatct cttctaatga tacatttttt tgcaggcatt
     8821 cccctactgt gattgctaaa ggtgtcgtca cattattcaa aatcaaaagc ggtacggata
     8881 actgtttttt aaaaaactga tggatttctt tcagagatcc catgtctact aatatgatca
     8941 atccatttga aacatcgctg ttttcgcaat aatcaaggat ttctgaagca atctgctgtg
     9001 gtgtgacatc tagtggcata tcaaatgatt caaatagatc ctcttgtaat aaacgattta
     9061 ctacatttgc catactgcta gcagttgcat aaccgtgagc agcaatcatc gcttttggca
     9121 gtcgtttttc ttttgaccat ttcgcttttt ttagatagat cgtcagtaaa atccgatcca
     9181 tacttgtgtc ttcaatatct aatttgatct tacttaattc cagaattcgt tggacatagt
     9241 gataactaga tggataagac tgagaaactt gttgttctaa ttctttgatt agttggataa
     9301 tatcatgttc ttccggtaac catttgatag tgcttcgctg atagagatag taactcaagg
     9361 catagacact gtttccatcg aatttgattt gataggcagt ttccatttgt cgaaaggtct
     9421 ctcggataga ttgtgtccaa taaaccagca gctcgtgttt ttcttgtcta ctggtctcaa
     9481 acagcaaaaa atcaaataat tgaatcactt cttgctttaa ttttccttcg caagaatata
     9541 gcagacagtt atttttcttg aaatcaatta ggatttgctc aaaactttta acgattcgct
     9601 gctgttcggg ctgttgcttc tccattaatt gctctaaggt ctggtcttgc gtaatataga
     9661 ttccttgctg tgctgtattt ttagtaatag gctgcgcaag tagttctttt tgcaaatgat
     9721 ggatcgtgac atggatttca ggctgttcac gctgttcagc atatgctttt gctgctgaaa
     9781 ctttgacgat atttttcagc tctcctatgt ttccggataa tttctgactt gttaaaagtg
     9841 acaaagctgg tccactgact gacaaagggc tccccatttt tctttgttct tcgagaaaaa
     9901 aagagtaaat cagttctagc cgttcattac gactacgctg attaagtgca ggtagttcga
     9961 ttcggatagg tattcttcga atgaaagttg ttaagaaatg actagtaatc tcttcagtcg
    10021 tagcaaaaaa gagacgtgtt ttgatccggc gtgacaaatt aggttctccc atacgataaa
    10081 tcacaccttg atcaagatag gtgaacagtt tttcctgacc ttctggtgtt aagcgatgta
    10141 cctcgtctaa aaacaagatc ccaccatctg ctgcttcaaa agcccctttt ttattttctt
    10201 ctgcaccagt gtacgctccc ttaacatgtc caaacaaatt gctagtcaaa agttcaggat
    10261 tatctgcata ttgtgcacaa ttaaaagtga tgaatggtgc agaatcttct agcagatcat
    10321 gaagcagaca atactgatag accaaattaa ctaaataact tttacctgtg ccactttcgc
    10381 ctgtaatcaa aagtggtaat ccgccatctg gatagtaaag agctgttttg atttgttcga
    10441 tactttcttt caaactggcg tcgtatccga tcataaaaga gaataagtct tttggttgcg
    10501 cgaaaaaagg ctgttcctct ttgagatcat cgatagaaaa ataaaaattc gtgcgtaact
    10561 taaaaaattg tttttcaaaa gcttctttgt ggaaataaat cactggtcga gaattgattt
    10621 tgactagtag ttgctcatca tttaactggt tcaaataatg actgactgtg tttcttttta
    10681 ccccaaaatg atcagctaac ctttgtgctg taaagacctc gttcatatgt tctaaatcga
    10741 aaaaggctgt ctggttctct aagtaacata aaatttcctt cttcaacaac ttattcaccc
    10801 ctcctccatc atagaaaacg gattcatttt gtcaataaaa tctttaat
//
LOCUS       DASJSZ010000088        10778 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.092, whole genome shotgun sequence.
ACCESSION   DASJSZ010000088 DASJSZ010000000
VERSION     DASJSZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.092"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            189..1004
                     /gene="thiD"
                     /locus_tag="U4B03_002312"
     CDS             189..1004
                     /gene="thiD"
                     /locus_tag="U4B03_002312"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEN2069131.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1333..2784)
                     /gene="cls"
                     /locus_tag="U4B03_002313"
     CDS             complement(1333..2784)
                     /gene="cls"
                     /locus_tag="U4B03_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN2069132.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3125..5791
                     /locus_tag="U4B03_002314"
     CDS             3125..5791
                     /locus_tag="U4B03_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2069133.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6155..6907
                     /locus_tag="U4B03_002315"
     CDS             6155..6907
                     /locus_tag="U4B03_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2069134.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6929..8083)
                     /locus_tag="U4B03_002316"
     CDS             complement(6929..8083)
                     /locus_tag="U4B03_002316"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEN2069135.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8268..10709
                     /locus_tag="U4B03_002317"
     CDS             8268..10709
                     /locus_tag="U4B03_002317"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEN2069136.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3517 a         1917 c         2309 g         3035 t
ORIGIN      
        1 attattagaa tagcgaatac aaataaacga ccactatttt ctgatttatt gcaaatgttt
       61 tttcaaataa atttgattac caagaaaagg tagtggtata tttactgagg gatactttga
      121 aaaacatatg atagaaacag taacagtatt atgaaaaatc tttttataaa aatggaggaa
      181 tcgatttgat ggaagatcag tatgtacaag caatgacgat cgcaggatca gatagcgatg
      241 gaagcgcagg gatgcaagca gatatgcata cattctttac aagaaatgtt tacggtgttt
      301 ctgtcatgac cgcatgtgtt gcaggtaatt cttacggaat cggagcaagt gtgacattgc
      361 caacagattt tatcgacaaa gaatttgaac tgattgcgaa ggactttcaa atacgtgcag
      421 ctaaaacagg tatgctagca gattcaaagt tgattgagac agttgtaaaa aattaccaaa
      481 aatatgattt tggaccatta gtcgtggatc ccgtaatcgt aacgaaacat ggaaatcttt
      541 tactagaaga atctgctttg caaagcttga aagaaaagct tgtccctttg gccgaagtac
      601 tgacacctaa tttttatgaa gcagaaaagc tgactggacg gacattagca gctgaagaag
      661 attttgtgca agcagcaaaa gaactgcaag atatgggtgc aaaaaacgtt atgatcaaag
      721 gacatcatgc taaaaatgcg cagcaagaaa cagtcaatga ctacgtattg ttcgaatcag
      781 gaaaatcatt ttggttaaat gcaccatttt atcctaccga ccggatcaac ggaactggcg
      841 attcgctatc tgcatgtatt gcagctgagt tagcgaaagg aaaaacagtt gaacaagcga
      901 tccgattggc aaaagactat gtaaaccgtg caattcgtga agaaataaat gtagggcaca
      961 aatatggccc catcaatcat tgggcagctg gtgaattaga gtaaaagaaa gaagttgtga
     1021 aaaagttgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
     1081 aacttggttg gacttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggtc
     1141 gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag cttctcatgt
     1201 ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tgttcggaag
     1261 aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt tttctctact
     1321 tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct tgaataatag
     1381 ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg tctcatcttc
     1441 tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag cacttgtttc
     1501 aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc agatttcatc
     1561 atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga tttgtacatt
     1621 ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag gatgatccgg
     1681 tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt ttaaagcagc
     1741 aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt ttttagcaga
     1801 agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg gtccgcttga
     1861 aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt ttttaaagaa
     1921 ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc agtctgttaa
     1981 gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg tatcgcgcca
     2041 atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac caccggtata
     2101 actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca atcggaaacg
     2161 aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt tgattagacg
     2221 attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata ttcgaacttc
     2281 cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga tattatctgt
     2341 cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat cttgaagcag
     2401 tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc ttgtcagcgg
     2461 catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat cgcccatgtc
     2521 aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta attctttcaa
     2581 gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga aaaataaata
     2641 taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc aaatggctgc
     2701 tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta atagataaag
     2761 ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata acattagtat
     2821 agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct ttcatgaaaa
     2881 tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag gatattagta
     2941 cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat atcaacagta
     3001 agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac aaatgcgtat
     3061 ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa atgggggatg
     3121 aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga tcgactcgtt
     3181 gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga aggaaaaatt
     3241 agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt ggaaattgtt
     3301 cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac tgattcagat
     3361 ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt tgaattctat
     3421 aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga aaaacctttc
     3481 agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg caaacgaact
     3541 tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg atggacgttt
     3601 actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt cgacttctgc
     3661 agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta caaatatggt
     3721 attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt caactgggaa
     3781 taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc aagtcactcc
     3841 gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt tggcattgtc
     3901 tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta caggagatat
     3961 gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag ctgctattcc
     4021 agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata aaatggcaaa
     4081 acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga caagtatcat
     4141 ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg actacttttt
     4201 aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag ctgacgaacg
     4261 tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag acggtactaa
     4321 attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa accagcctta
     4381 tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact cagatcgaaa
     4441 attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta aaggcggacc
     4501 agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg ttccgatgac
     4561 ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac gcgcattacg
     4621 tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg aagatgaaga
     4681 cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg aagtgactca
     4741 ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg gtgaccacaa
     4801 aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa acctggcttt
     4861 gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt tagaaaaagt
     4921 cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg cttggcaaaa
     4981 taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag cattgaaaca
     5041 agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg cagcagcgat
     5101 ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag gacgtaatgt
     5161 gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag gtgcgatcat
     5221 tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat tacaactgtt
     5281 gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag aaaaagctga
     5341 acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta cggacaaaac
     5401 attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct ttgctcaatg
     5461 gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa cattgatttg
     5521 gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta tccaagcagg
     5581 gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc tttactgctt
     5641 aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg gattgacaga
     5701 agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa tcactaaatt
     5761 gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt gagcctgccg
     5821 tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt gttgaatatt
     5881 gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt tgtaaaatca
     5941 gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg aaagagggac
     6001 tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa agccaatcat
     6061 tttattattc atcaaaagag atagaataga taaaagagga gaatgattta ttccgaaaag
     6121 tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat gggatgaatt
     6181 tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg aagatgtaaa
     6241 aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg ttgctggagg
     6301 atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga tcgatttttc
     6361 ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc caaatgtttc
     6421 ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg aaatggtttt
     6481 tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga tgaaaaagtc
     6541 aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt tttatcctat
     6601 cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg taatggatca
     6661 atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt ttacttattt
     6721 tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt acgcaaaaga
     6781 tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa tccttagtac
     6841 gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa cctttcacga
     6901 ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata tcattttcga
     6961 tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg tgttcaggaa
     7021 gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga gaatctagca
     7081 tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga taacctgtaa
     7141 aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct cctggctgta
     7201 gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca gtgtaaagat
     7261 taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga cgttcctgat
     7321 tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc ttagtatacg
     7381 gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat tctttatgtc
     7441 gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga ccatcaacaa
     7501 caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc agaaatacgc
     7561 tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca acagcaccga
     7621 caccttgcgt cacttcacca ccgcttgcca agctgtaacg agcaatatca cttgctatga
     7681 ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc aaagctgcgg
     7741 tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg attttcagta
     7801 aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct gttccgacga
     7861 tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca gcatttgctc
     7921 ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga ccaatcccta
     7981 tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata tctaaatata
     8041 aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca cctctttact
     8101 tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa tataaaaaag
     8161 agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa caagagctat
     8221 ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg aaagaagtcg
     8281 ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta agtccattta
     8341 ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca aaactgaaaa
     8401 aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat ggtcaaaatg
     8461 ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca atgacgatca
     8521 atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg atccaattag
     8581 gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca cctatgctga
     8641 aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg gtcaacgacg
     8701 gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat gttgcaacac
     8761 aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag ttgaaagcag
     8821 cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag atttctgatg
     8881 aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa aaactgggca
     8941 cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct tccccgttga
     9001 atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat aataatctgc
     9061 cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt atcatgggaa
     9121 tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat ttatcaacaa
     9181 tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt agccaagaac
     9241 tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta ggccatccaa
     9301 ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt cgtgaacaga
     9361 agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca gtcttgttag
     9421 aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat cagcttacgc
     9481 catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa actgcgttga
     9541 tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa aaccaagtga
     9601 gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc aagaaaaaat
     9661 ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat ggtgcaaaaa
     9721 tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa atcgttctat
     9781 caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa gaagagctga
     9841 ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt gttcaagata
     9901 tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat tttttagtgg
     9961 atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt gtggcaaaca
    10021 agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt aattttgcta
    10081 cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg agaaacaaag
    10141 aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg aagcttgacc
    10201 cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct gtagtggcag
    10261 ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg gcaagaaatg
    10321 gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt ggaaaactaa
    10381 cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca aaagcaaaag
    10441 catctttagc tatgttagat attgattcag ccaaggaact agcacaagta attgctgcag
    10501 tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga atccaaaaag
    10561 gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt ggtgaggaaa
    10621 tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag acagctatac
    10681 aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga ataaagcaag
    10741 atttacagtt attttattta ctgcagatct tgcttttt
//
LOCUS       DASJSZ010000089        10315 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.093, whole genome shotgun sequence.
ACCESSION   DASJSZ010000089 DASJSZ010000000
VERSION     DASJSZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.093"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            229..378
                     /locus_tag="U4B03_002318"
     CDS             229..378
                     /locus_tag="U4B03_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEN2069137.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            389..1903
                     /gene="dltA"
                     /locus_tag="U4B03_002319"
     CDS             389..1903
                     /gene="dltA"
                     /locus_tag="U4B03_002319"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEN2069138.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1900..3105
                     /gene="dltB"
                     /locus_tag="U4B03_002320"
     CDS             1900..3105
                     /gene="dltB"
                     /locus_tag="U4B03_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEN2069139.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3150..3383
                     /gene="dltC"
                     /locus_tag="U4B03_002321"
     CDS             3150..3383
                     /gene="dltC"
                     /locus_tag="U4B03_002321"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEN2069140.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3386..4654
                     /gene="dltD"
                     /locus_tag="U4B03_002322"
     CDS             3386..4654
                     /gene="dltD"
                     /locus_tag="U4B03_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEN2069141.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4795..5877
                     /locus_tag="U4B03_002323"
     CDS             4795..5877
                     /locus_tag="U4B03_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEN2069142.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5877..6680
                     /locus_tag="U4B03_002324"
     CDS             5877..6680
                     /locus_tag="U4B03_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN2069143.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6750..7490
                     /locus_tag="U4B03_002325"
     CDS             6750..7490
                     /locus_tag="U4B03_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2069144.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7501..8505
                     /locus_tag="U4B03_002326"
     CDS             7501..8505
                     /locus_tag="U4B03_002326"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEN2069145.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8550..10247)
                     /locus_tag="U4B03_002327"
     CDS             complement(8550..10247)
                     /locus_tag="U4B03_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069146.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3309 a         1704 c         2091 g         3211 t
ORIGIN      
        1 tttggaataa gaggttgtga cacgattttt gtcacaacct ctttattttt tatatttttt
       61 aaaagtacga gaaattataa agaaaaaagc agtgatttga tgtgctattt ttataaaagc
      121 tatgtgacaa aactgttagt aaaaaaacgg tttctgtaag ctagacacat gtttcttttt
      181 gtcaaaatta gttacacttt gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg
      241 cgttctaatc caaaattgaa ttattgggga aaattcatca ttcgaacaat tttttatagt
      301 gcagtactgc tgttcctgat ttatctgtat cactataaaa atattcaagg aggaactttt
      361 atctataacg aattttagga ggaagagtat ggaaatcaaa acgattattg aagcaatcga
      421 tgattgggga ataaatgaac ctgatcgagt tgcttatcag gcggatgaga cgcatacttt
      481 tggtgaattg aaacaagcat cagacgcact tgcttattat ttagaaaaaa aggtagaagg
      541 cgatggacca attgtcgttt ttggcaattt ggagtttgaa atgattgtct catttctagg
      601 ggttgtcaaa tctggccatg cctatttgcc gattgaagag catacaccca aggagcgtat
      661 cctatcgatc tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg actggataga
      721 agatattcct tcggttcctg tcataacaaa aaaagaattt caagaaatca agaagatacc
      781 agtaggattt tatcccagaa atagcgtaaa aggtacagaa aatttttata ttatttttac
      841 ttctggtact actggagaac caaaaggcgt acaaatcagt catgataatc tagtcagctt
      901 tgttcaatgg acgatggaag actttgggat caagcctggt atgcatttct tagcccaagc
      961 gccattttct tttgatttat cggtattttc tatttatcct gcattagttt ctggcggaat
     1021 gctgaagccg ttaaataaag cagttgttca agactttcgt caattattcg cgacattgcc
     1081 agccttgaag ttgaatgtgt gggtttctac tccatcattt atggatattt gcttgatgga
     1141 acctacattc aatggagaaa atgtgccgga attggacatg tttttatttt gcggagaaga
     1201 attgacgaaa aaaactgcag aaagtctact tgatcgattc ccaaatgcac ggatatataa
     1261 tacttacggt ccaacagaag caactgtagc tatttcaagt attcagattg accagcatgt
     1321 actagatagt tatgaccgac taccgattgg ctacgtaaaa aaagacacga aagtttctat
     1381 tgtccaagaa ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc
     1441 aagcgtatca aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca
     1501 aaatgactta gcaagttacc atacagggga tgcaggtaga ctagatgaat atgggctttt
     1561 attttatgaa ggacgtatgg atcagcaggt caaattacat ggataccgga ttgaactcgg
     1621 ggatatcgaa cattatctgc tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa
     1681 ataccaagga acaaaagtac agcagctggt tgcttttgtc gtattagaaa acaaaacgaa
     1741 acagccggat tttcagttga ctaaatctat caaagaacaa ttactagaag ttgtaatgga
     1801 ttatatgatt cctcagaagt tccattatgt cgatctgctg cctcagacag ttaatggaaa
     1861 aatcgatcga aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc tcatatgatc
     1921 ccttatgcag agccgtttta tttttttctt ttagccattg cgcttgtacc gatcgttcta
     1981 tctttattga tatgggaaaa aagattacca gtttatcaat cagcagtgac acttttcttt
     2041 ttatttgtca gctttggagg taattattgg caacagggag cggcgctgat tgggtatgtt
     2101 ttgtggcaga cgatacttgt atggggctat tttgtttatc ggcaaaggaa aaatcagaca
     2161 ggatggtttt ttgccgccgt tattttagcg ataattccac tgtttttaac gaaagtgact
     2221 cctttttttg aacatggaaa agcatcgtta cttggttttt taggaatctc ttatttaacc
     2281 tttaaatctg tacaaatgat catggaaata agagatggca tgatcaaaac atataagcca
     2341 tttcgttata tccagtttct gctgttcttt ccaacaatct cttctggacc aatcgatcgc
     2401 tataggagat ttgaaaaaga tcagctccat ccgcctgttc gtgataaata tgttgatctt
     2461 ttgggaaaag gcgtccaaaa tatttttgtc ggttttttat ataaattcat tatcggttat
     2521 tattttggtc aagtactgct tcctgtagtg ggacgcattg ccttacagca tggtggtatt
     2581 tcatgggcat tagtaggtta tatgtacgtg tacagtatgt atttgttttt tgattttgct
     2641 ggctatagct tatttgcagt cggtacaagc tacatgatgg gatatcagac accaatcaat
     2701 ttcaataaac cttttttatc atggaatatc aaagaattct ggaatcgttg gcatatgacg
     2761 ctgtcctttt ggttccgtga ttatgtatat atgcgattga tgttcttcat gatgaagaaa
     2821 aaatttttta aaagcaagat cgtaatgtca aatatcggtt attttgcttt gtttcttcta
     2881 atgggtatat ggcatggatt gacgtggtac tacatcgtat atggactgta tcatgcttgt
     2941 ctgatctgtc tgacagatgc atggatccga tttaagaaga agcataaaga gcagttgcct
     3001 tctaacaaat ttacgcatgg cttagccgta tttttgacat ttcaagcagt atgttttagc
     3061 ttcctgattt tttcaggatt tttagataaa ctttttttta gctaacgaat agtgacatat
     3121 taatttatat aacaaaggag aaaaggaaaa tggaagaaca aattttatca atcttagaag
     3181 aaataacagg aacagatgaa gtaagaaaaa atcgtgatat tgatctattt gatgaagggt
     3241 tgcttgattc tttagcatct gttcaattat tagtcgagct agacggagag ctggatatcc
     3301 aagtccctgt atctgaattc gagcgagagg attggtgcac accgaataaa atcattcaac
     3361 aagcaactgc attgaagggt tgagtatgaa aaagaaactt tttgctattt tcggcccggt
     3421 tgttttagct gttggcttac tgtttgtgtt ttttacctcc tcctatcaag tagatatctc
     3481 taatccagaa acgattagac gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga
     3541 tgcgataaaa aattatgcat tatctgaaaa acaatatatt cctttttttg gctcaagtga
     3601 attaagcaga atcagtccat ttcatccgtc tgttctggca gaaaaatacc agcgaaacta
     3661 tcgtccattt ttgctgggag cacccgggac acaatcgtta agccaatata tgatgatgcg
     3721 ttctgcagga gatgctatga aaaacaagaa agtcgtcttt attatttctc ctcagtggtt
     3781 cgttaaaaat ggtgtaaaaa ctgattactt taatacgtat tattctgaac ttcaaacata
     3841 cgactggttg ttttcaatga agaaagtaac gcctgctgat cgctatttag ctagaagatt
     3901 attaactttc tcaaaagtaa aagagaatga tacgttgacg gcgattctgc aaacgatcaa
     3961 aaaagggaag ctacctttac ctgaatcttt gaatcagctg cgcagccaat ggaacatgct
     4021 gaagagagag gatgaagtat tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga
     4081 tcatgagtca aaacgattac caaaacaata ccaagaaaca gaattatcca ttttagcaaa
     4141 tcaaattgga gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg atttttatac
     4201 gcatcgtatc cgagcccacg agccagagct caaacagtca caaaaaaatt gggattatcg
     4261 tttttcgcca gaattttctg attttcagct ggtcttggat caattagcaa aaaaccataa
     4321 cgaagtattg ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc
     4381 tcaagaaatg ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt
     4441 caatcgaata gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat
     4501 ccatctaggc tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga
     4561 aaatcatatt actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca
     4621 acatcaaata ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt
     4681 gttttcttta tttttgaata ttctgaataa aagataaaaa taccaagttt gcctgaacaa
     4741 aaatcataag caatggtata atcacaatat gaatatagaa atgggggaaa acatttggat
     4801 acaaaagaag aacagctact tattgattta acttctgcaa aaggagtacc tggaaatgaa
     4861 gaagaggtaa gagaagtatt cagagaatat gccaaaccat tcgctgatga tattttttat
     4921 gacggtttag gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa ggtctttatt
     4981 tctggacata tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa aggattcttg
     5041 gaattccaaa ctcttggagg ttggtggggg caagtcatgc ttgctcagca agttgaaatc
     5101 aaaacacgtg aagggaaaat cgtccacggt gtgattggtt ccaaaccacc acatgtttta
     5161 acacctcaag taagaaataa accttatgaa ataaaggata tgttcattga tattggcgca
     5221 tcaagccaag aagaagccaa agagtggggc atacgtcctg gagatatggt cactccatat
     5281 attgaataca aacgaatgaa tggcagtaaa tatttactgg caaaagcatg ggataatcgt
     5341 atcgggacag ctgtttctct gcgagtatta gagaatctat ctaaagaagc acacccaaat
     5401 gttttattcg caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt
     5461 acacaccttg taaatcccga tattgctttt gctttagata ctggtacagc aggagacact
     5521 ccaggaatga caccaaaaga agcagattcg attttaggaa aaggcccaca gattttgatt
     5581 tttgatgcat ctatgatccc gcataaaaaa ttattgaatt ttgttattga tgtagcagag
     5641 gaaatggata ttccattcca atatacagtg atttctggcg gaggaactga tgcaggacaa
     5701 atgcatttga caagagatgg tgttccgtct gtagccatta ccgttcctgt tcgttatctg
     5761 cattcccata catcagtgat tcatgaagat gattatttga atacagtgaa actagtcaca
     5821 gaattagtga aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga
     5881 ataatagaat acctatttct gaatatcgga aaaaagcacg agtttctttg gaaaatcaat
     5941 ggggaatcaa tacttggctt gttttcttat ctataatatt tacgagcgtc atccagaact
     6001 tttttgacgg tttcagtcct tttccggaag aaagcatgca gcagcactta ttgagttttc
     6061 tagtcgataa tttactttta ttcgcattca actatgcatt gttttacatt gcgctagtag
     6121 ttgtacgggg tggcagacca aaaagtaacc ttttattttc agttttccaa agaaagtatt
     6181 ttggttcgat acttggcatc aatttgatca attcagtcat caactggcta ctcaacagtt
     6241 taatctttct gcccatgttt ttaatcagcg gagtgagtac ttatatgcag ctgcttttca
     6301 gttcaaatgc ggtacctacg ggtgtcatgc cagaagattt tcttgatatc agctttgttt
     6361 tatcgatgat agggatgatt tttgtttcag cactcatcat gagtattgtt ggcggtttat
     6421 tccaatttgc tgcatggaca aagttcgact atcctgattt gtcggttatt caatgtttga
     6481 aatatggctg gtttttattg aaagatagaa ttggtacgta tattttgttg cagctgtctt
     6541 ttatcggctg gtatttgcta ggagcaatcg ctttgttcat cggtcttttg tgggtcattg
     6601 tttatgtgaa tgtgtcagtt gcagaatttt atgaacaagc acggattgaa aaaggaggtc
     6661 cagacgccta ttttacttag catgtaagca gtttattcat caaaaaaata acttttgtgg
     6721 taggataaat ctttgagggg gaatgtacga tgaaattaac tgttttagga tgcttaggag
     6781 cgtatcctta taaaaatcaa gggacaacag gttatttgct tcaatctgac gggttcaatt
     6841 tgttgatcga tgcaggtagt gctacgctga tcaaattaca agaacacctt gatccacttg
     6901 atctagatgc tgtgatcctt agccattatc atcatgatca tattgctgac cttggtgtat
     6961 tacagtatta ctggcaactt catcctaaga gaaatccgga gacattgctt tcaatttatg
     7021 gacacacaaa agattccatt cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac
     7081 atccttattt tgaagctgaa gaattaaaaa ttggtccttt tctgattact tttatggaaa
     7141 caatccatcc agtagtatgt tacgccatgc gtatcgtaga agaaacaacg gggaaagtat
     7201 tcgtatttac aggtgattca ggttatttgg aaagtttcgc tgattttgcg aaagatgctg
     7261 atttgtttct agcagatacc tatctttttg aaggaaatga acatcatcat gcccatttca
     7321 cttcaaaaga agctggagaa cttgcgaaat cagctaacgt aaagaaactt gtacttacac
     7381 acctacctca gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca
     7441 aagggattga tatagaactt gctgcagttg acaaagtgtt taacatttag gaggaagaac
     7501 atgatttttg taccaaatga aaaccacgac ccaagaatca atttagcaat tgaacaatat
     7561 ttgctgcaag aaatgccttt agatgaacct attttgctct tttatatcaa tgaaccttca
     7621 atcatcatcg gcagaaacca aaatacgctt gaagaaatca atcgagatta tgtggaagaa
     7681 aatggcattc atgttgtccg tcgcttaagt ggaggcggag ctgtctacca tgattttgga
     7741 aacctgaact ttagttttat catgcctgat gatggggatt cattccgtga ctttgcgaaa
     7801 gtaacacagc caatcatcca agcacttcat gaattgggtg ttgctggggc ggaattgaaa
     7861 ggccgaaatg atctagtcat tgatggcatg aaattttctg gcaacgcgat gtatgctacc
     7921 aacggtcgga tgtttgctca tggaacaatt atgttcgact ctgatatcaa tgaagtagtc
     7981 aatgcattaa aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc gatccgttct
     8041 cgtgtaacga atatcaaacc atttcttcca gaagaaaagc aaaaaatgac aacagaagaa
     8101 tttcgtcaag aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg
     8161 tacgaattga ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga atattatcga
     8221 aattgggatt ggaattacgg gaagtctcca gatttcaatt tctctcgcca aaaacgtttt
     8281 tctatcggtt cgattgaagt ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa
     8341 atctttggcg atttcttcgg actaggagat atctctgacg tggaaaaaaa attggttgga
     8401 cagaaatatg ataaagcatc tctatcaaaa atagtcagcc aaattgatgt gaagaaatat
     8461 tttggtgcga tagaaccaga agaattgctt accttacttt attgaaaata aaaaatagcg
     8521 gaatccacat atatggattc cgctattttt tatacaggat tcgggtaatt tcgagagagt
     8581 tgaacagtga tccgatcaat gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga
     8641 tccgctacgt ctttcatcat cattaagtca aaacgttcct gatcatctaa ttcttctgtg
     8701 atgcctcgac taaataggtt tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa
     8761 tgttcctctt gttcgtgtat cgacaaataa atttttcgat acagtggatt ttcgatggca
     8821 tgagtttctc taaaggaatg ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg
     8881 ttgataggaa gttcttcggg aagatctgct tggaaggtta tctgaatatt ccgtttggca
     8941 gctaacttct ggtaacgcgc taagataaga tttgtcacct ggtggttgca aaaagaacac
     9001 agcatttcga aattttttgc ttcttctaat atcttttcca catagttttt agcttttgtt
     9061 tggtccgtat gcattaaatc ttgcagattc tccatctctt ggacaaattc caggcgaagt
     9121 gtatttactt cttggatatg ctgtcgaagc tgctcataat gttgctccaa caaatcattt
     9181 ttgattttta agaaatctac ttgtctccga tctttgtcgg tatttaagat gtgatcgatt
     9241 agattgtagc tgataataac gattagaatg ataatatata aaatcgtaga ccaattgatc
     9301 gttgtttgat agatacctaa tgccgtttgg atgtaaatca agcaatggat aatggctacc
     9361 aagacgatcc aaggtgtaca agctacataa aaacggttat tcttatatgc ttcgccaatg
     9421 catgcaagtg aagtgactaa agtgcttata acactagcaa tattagcaat cggcattaat
     9481 tcaggaagtt ctgcaggagt aaaagttgca taaagcagaa cgccgataaa aatcaaaagt
     9541 tggaacgtac aaaaaattcc gtaccatttt ttatagtaaa acattcggct ccaatagtag
     9601 cagatgatga aagctgacgt taagataata aataaatgag ataaaaaaga atgaacgaat
     9661 ggttcaaaaa gaataccact taaaatatct tctgatacag cttcaaatcc taatgccaaa
     9721 gcaaagcaac ttaagagaat aagtgagtat tcaagacgtt tttgtttata catcaagtag
     9781 atcgtataga aaaaggtccc tatagcgata aaaacagcga ttatcatcgt caatacttgc
     9841 ggcactgatt gtgaataaat aaaactaatc aatggtccag ttgttcctag ataaactttc
     9901 ggtggcagtc cagcatagtt ctgataagga gatgagactg taagggtcaa tttttttccg
     9961 atataatttg ctggtaaatc tatcacagcc aatgagagtc cgggattgct ttcttcctga
    10021 taatctttcc gttggtataa aatcttttcg cctagatata cagtaatcca ttgatggttt
    10081 cctttgatta gtaaatttgc ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat
    10141 gtttcgtctt tatccacggt aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca
    10201 ggatcatctt ttgtatagaa aacccacgaa tcttttattt ctttcatttg attgctgtcc
    10261 gcaggagaaa aaataaaata aaagaaataa gcaattcctg ttaaaaataa caatg
//
LOCUS       DASJSZ010000090        10301 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.094, whole genome shotgun sequence.
ACCESSION   DASJSZ010000090 DASJSZ010000000
VERSION     DASJSZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.094"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            497..766
                     /gene="rpsN"
                     /locus_tag="U4B03_002328"
     CDS             497..766
                     /gene="rpsN"
                     /locus_tag="U4B03_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN2069147.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            781..960
                     /gene="rpmF"
                     /locus_tag="U4B03_002329"
     CDS             781..960
                     /gene="rpmF"
                     /locus_tag="U4B03_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN2069148.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            992..1369
                     /locus_tag="U4B03_002330"
     CDS             992..1369
                     /locus_tag="U4B03_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069149.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1390..1539
                     /gene="rpmG"
                     /locus_tag="U4B03_002331"
     CDS             1390..1539
                     /gene="rpmG"
                     /locus_tag="U4B03_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2069150.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1563..2546
                     /locus_tag="U4B03_002332"
     CDS             1563..2546
                     /locus_tag="U4B03_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN2069151.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2576..2698
                     /locus_tag="U4B03_002333"
     CDS             2576..2698
                     /locus_tag="U4B03_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN2069152.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2716..4239
                     /locus_tag="U4B03_002334"
     CDS             2716..4239
                     /locus_tag="U4B03_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN2069153.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4263..4502
                     /locus_tag="U4B03_002335"
     CDS             4263..4502
                     /locus_tag="U4B03_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069154.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4695..5156)
                     /locus_tag="U4B03_002336"
     CDS             complement(4695..5156)
                     /locus_tag="U4B03_002336"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2069155.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5351..5569)
                     /locus_tag="U4B03_002337"
     CDS             complement(5351..5569)
                     /locus_tag="U4B03_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069156.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5687..7303
                     /locus_tag="U4B03_002338"
     CDS             5687..7303
                     /locus_tag="U4B03_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN2069157.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7544..8884)
                     /locus_tag="U4B03_002339"
                     /pseudo
     CDS             complement(7544..8884)
                     /locus_tag="U4B03_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9160..9315)
                     /locus_tag="U4B03_002340"
     CDS             complement(9160..9315)
                     /locus_tag="U4B03_002340"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN2069158.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9611..9943)
                     /locus_tag="U4B03_002341"
     CDS             complement(9611..9943)
                     /locus_tag="U4B03_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2069159.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3440 a         1622 c         2024 g         3215 t
ORIGIN      
        1 atttttggga tatagttata attctttttc aaaaagaata attttattaa acagcatcta
       61 attttgaata cacgaaagaa tcagtatgat tgaattagtt cacttaatgt ctattcttat
      121 aatataagga ataatcaaag ttttaaaaaa ttggcgataa accagttaaa cataaaagat
      181 tgaataaaaa gttatagttt ctttcatatt gccgttttaa gtagaggaaa agctacttaa
      241 aacggtattt gtgtttgatt ggactaataa aagtgaattc ttatttttca gagcctaaac
      301 tgcatattct tttaaatgat ttgattatct ctatgtaccg ataaattcgt tttcaaatag
      361 ttatctattc atatgagttt tatttcatgt tatttattgt tgacaagagc aactcttgtt
      421 ggtaaagtat cagtgtcaaa acgtaacgat tacgatttga ggtgtctaaa tcaaaataaa
      481 aggagtataa aggtatatgg caaaaaagtc gaaaattgct aaagcaaaga aacaaatggc
      541 gatgattgaa aagtatgcag ataaacgtca ggaattaaaa gccgcaggag atcgcaccgc
      601 tctcgctaaa ttgccaagag attcaaatcc gaatcgtctg aggcttcgtg atcagacgga
      661 tggtcgtcca agagggtata tgcgaaagtt cggtatgtcc cgtattaagt ttagagaact
      721 tgcccatcaa gggttgattc ctggagtaaa aaaagcaagt tggtaaaagg aggaaatatc
      781 atggcagtac cagcaagaaa aacatctaag gcgaagaaac ggttaagaag aactcatcaa
      841 actgtagtaa aaccagaaat ttcatttgac gaagcgaatg gtgattatag acgtagccat
      901 catgtatctt taaaagggta ctataatgga aagaaagtaa ttaaaggagc ttcaaaatag
      961 tttaatatat aacttttagg agggaatcaa aatggaaatt atttacccac ctttagttga
     1021 agaaggatta aagtattatc ttgaaacgac ccagcaatcg ctagataaat gcacttttta
     1081 tcgttcgatg gtagagagag gaattattac tgaaacgggt ttaccgaccc aacaggcaat
     1141 tgaaaatgga ttagtcaaag attactatga ggaccaaggc ttatcatttg atgatttttt
     1201 aagaatctat ccaatttttg aagagtatga cgaggagtta tttcaatgta tcgatggtta
     1261 ttgggagata cctgttgata tgaaagaaaa tttagtttca caattggaat ctggcgagtt
     1321 aacttttgaa gatgcacaac aaattcaagc atatcttgaa gatcgataag tattgaagga
     1381 ggaagattta tgcgtcaaaa tattattttg gaatgtgtcg aaactggaga acgtctgtat
     1441 ctcaccagca aaaataaacg gaataaccct gaacggttag aattaaaaaa gtattcgcca
     1501 aaattgcgca gacgggcaat ttttaaagaa gtgaaatagc taagtggtaa aaaggtgaca
     1561 aaatgggcat accagttaca atcgtttctg ggtttttagg ggctggaaaa acaacgctaa
     1621 tcaatcaagc cttgcaagtc tcaccctacc ccagggaaga aattattata attgaaaatg
     1681 agtttggtga agttgggatt gatcacaaat tattagttca tagcgaggaa cgggtgttac
     1741 aactaaataa tggatgtatg tgttgcagtc ttcgcagtga tttattagca atattgattt
     1801 ctttactgga aacaatagaa gaacatcaac aaccaatttc acaaatcatc attgaaacta
     1861 caggtatagc agatccccaa ccaatcattc aaacactttt aacagcgcct caactaagag
     1921 gaagagtata tatcgatagt ttattgactg ttattgatag tgacagtttt gaacttattg
     1981 aaagagaacc acaagccctt aagcaacttg tcatggcaga tcgcattttt gtgtctagaa
     2041 aacaaaactt gaatttatct gcactaaaaa aattacagag agaaatcaaa agtattaacc
     2101 ctttatccga tattcgaatg ttcacacata gtgaaacgat tcaaaaaaag gatttttata
     2161 atttgaaaaa atttaatcat cccttagttt ttgaaagtga ttcatcagaa aatcactata
     2221 atggcaaata ctatcatcaa caccatcaag tacatgagtt taaatcaatt ctcttaactt
     2281 cagatcaaga cataaaaaaa gattttttac tccaatggat tgactggctg attttaacca
     2341 accaagggag cctgtatcgt tttaaaggat tgattgcttt acaagagcag gattttatag
     2401 ttgccatgca agaggtaaac gaacaggtta attttcaata caccactcga caaaaagacc
     2461 aaaccactat tattctaatc ggaaaaatgt tggatgaaga aaggatcaaa cagagttttg
     2521 ataaattact ggcggaaagc aaataatgat aagtagtgat tgggggtaat ttctaatgga
     2581 gaaaacggat atatctagtg catatcgacg acttaaaagc ccgaatatta aaactagaaa
     2641 aagggcatta aaaattatca aagaacataa acgaaataaa atgaagaaat tagcataatt
     2701 aaggaaggaa aaagaatgaa gaaaatcagt gtaggactta ttgggatagc agctctagga
     2761 ttattgggag cttgctccag cactaatgat gcaaaagtat cgaatgataa agatggaaaa
     2821 ttagaaattg taacaacatt ctatccaatg tatgatttca ccaaaaacat cgtcggcgat
     2881 gaagcgaacg tggatttaat ggttccagcg ggatcagaac ctcatgacta tgagccctca
     2941 gctaaggata tggcaaaagc tcatgatgct gatgtttttg tatatcataa cgaaaacatg
     3001 gaatcatggg taccaaaagc taaagaaagt tggaagaaag ctgggcctaa tgtagtagaa
     3061 ggaacaaaag acatgatttt attgccaggt agtgaagaag aagatcatga tcacggagaa
     3121 gaagatcacc atcatgaact agatcctcat acgtgggttt cacccaaaat ggcaattaag
     3181 gaagtttcaa atatcaagga tcagttagtt aaattgtatc cgaaaaaagc aaaagtattt
     3241 gagacaaatg ctgaaaagta tttgactaaa ctgaaacggt tggatgctga ctataccact
     3301 agtcttaaag aagcgaaaca aaaaagtttt gtgacgcaac acgcagcctt tggatattta
     3361 gctttggatt atggattgaa acaagttcct attgctggac taagtcctga agaagagcca
     3421 tcttctggtc gattagctga attaaaagag tatgttaaaa aaaataagat caattatatt
     3481 tactttgaaa agaatgctaa tgataaaatt gcaaaaactt tggccaacga agctgggata
     3541 aaattggaag tactgaatcc tcttgaaagt cttacaaaag aacaaatgga taatggtgaa
     3601 gattatgttt cagtaatgga agacaatcta aaagcgttag aaaaaacaac tatggttgct
     3661 ggtgaagagg tagttccaga aaaagaagcg aaggatgaaa agacagttgc taatgggtac
     3721 tttaaagatg ccgatgtgaa ggatcgcgaa ttgagtgatt atacaggcga atggcaatct
     3781 gtttatccgt tgttaaaaga tggaattttg gatgaagttt tcgattacaa agcaaaaatc
     3841 aataaagaca tgacagctgc cgagtacaaa gattattata ctactggcta taagacagat
     3901 attgatacta tcaacattaa agacaacacc attgattttg tagtcaacgg tgaacatcat
     3961 caatacacat acaaatatgt tggatacaaa attttgaatt atgaaaaagg aaaccgtggt
     4021 gttcgtttta attttgaaac tgatgatgca ggtgctggtc gttttaaata tattcaattt
     4081 agtgatcatg gaattgcacc aagtaaggca gagcatttcc atattttctt tggtggagaa
     4141 agtcaagaaa aactctataa tgaattgcat aattggccta ctttctatcc agcttcattg
     4201 tctaaacatg aaattgctca agaaatgatg gctcattaaa attcaatttt tggaggaaat
     4261 acatggtaag aaaagtatta ttgtctatgg attccaagaa tattgatgct tatcggcgtt
     4321 atcgatttgc aaagcaaatg agattaaatg ggagtacatt agctgatatt gctgcaactt
     4381 tacatgttag tattgacaca gttcatagag tattaaaacg ggatcctgat tgttgtttta
     4441 tcaaggtcaa tactggaaga agtagacgtt tagcaaaacg gcataatagc ttggccaatt
     4501 aattgataat accagagtag atcatttttc ccttatttgg atttgatctg ctttttatta
     4561 tgtcttgaat tgtcaaatca agcgcaacac tttatcaaag aagacttcat aaggtgttct
     4621 ccaatctaaa cactttctag gtctgttatt cagtttttca atcatctgat ttatcgttga
     4681 gttatctacc gtagctatct ctttcgattt tggcaagtat tctcgaatga gtctattcgt
     4741 attttaattt gttcattgtt gccaagggca tgaggatctg gaaaataaaa gggcatatta
     4801 ttcaatgctt ctgtaactgc gaaatgattg gagaattctt ttcctctatc tggagtgatc
     4861 gttttgaccg cctcatttgg aatactgctt aaaagttcaa tcattttctc gctcacataa
     4921 gcggctttct ttctggggac cttctttcct gctaaaagga agcatgattt tctgtctgtc
     4981 aaagtcacta aacaagtacc accagttttg ccagctacaa tatcggcttt ccaatgacca
     5041 aaatcacttc tatcatctgc ggattttgga cggccatgga tcgaatgagt aaggcaaatc
     5101 tttccacgag actcgacatg attttttgta tgtcttgttt ttcctcgatg gtgcaatgaa
     5161 cggatcaggc ctttattgcc gtgtgagagg ggttctgtat cgaaaagatc attgcaaatt
     5221 gcacgttaga tcgtgataaa actaattgaa aagtccatat gttcatgctt caaacgattc
     5281 gagatttcct ctggcgacca ctgaaaatcg acaaataaat gacgaacaat tgaccaaact
     5341 cacggatcat ttagaatgtg ataacgtccg catttttttc ttcgataagc ataatttttt
     5401 tccgctttgg aaggtgaata gtttttttct tgtttcttct cagttcatgt aaaatagtaa
     5461 acgggctatg ctgaatgtcg ttgccaatta tactaataga aaatccttct tcgtgaagca
     5521 gaaagattct ttctcgttcg agtatagtaa gatgacgata ctggctcata attattttcc
     5581 ttctaggagt gttctcgaca aacttatact agttggatat cgattatgac tcatttttat
     5641 gtgttgcact taaagtgtaa attcaaggta tagaaagagg ttccctatga aaaaaatacc
     5701 tattacaatc ttaactggct atcttggttc aggaaaaacg atacttctaa atcatattct
     5761 gtacggtgat cacggaaaga aaattgctgt cattgtaaat gacatagggg aagtaaatgt
     5821 tgatggggta ttagtcgaca agaaaggttt taaaagaact ggagaaaaac ttgtttcgat
     5881 gccgaatgga tgcatttgtt gtacattacg cgaagatttg attgttgaac tagatcaatt
     5941 agtgcaattt tctgacttga atcaaattgt tattgaagct tcaggaatca gcaaaccaat
     6001 tccaattatt caagcaatta tattagctaa aaacccttta gacattgatt tgacccaacg
     6061 attagagatt ggtgccatta ttatagtagt ggatgtaaaa cgtttttggg ataatttcga
     6121 aacgggtgaa aagattcagg aaagaaaagc tgaaggtaat gtagttgaag aagaacagga
     6181 tattcgtggc ttgttaattg atcaattcga gtgttgtaac atattacttt taaataatag
     6241 tgacttagtg tcaaccaaga cgatttacaa aattaacggt ctaattgcta agttacagtc
     6301 caatgccaaa ataattagga caattaaagg tgaagtagac ttatcgaaaa ttctaaatac
     6361 aaatcttttt aatatagatg agacttccta ttcagtgggc tggttcaaag agttagagct
     6421 tggctatcaa aatcatattc ctgaaacaga agaatatggt atctcaagtt ttatctatcg
     6481 aaatcggtta ctccttgacg cacaaaaatt tgcccatctt acggataaaa attttcctgg
     6541 caatattaca agagcaaaag ggtttctttg gtttgcccaa catagtgatt tttcgacgat
     6601 ctttgaaaca tcgggtagct ataaaagtat tgaatcatta aattattggg tagctagttt
     6661 acctaaaatt gagtgtcaaa tgattataca aaaaaatccc gaattgaagg aagcctggca
     6721 tccgttgttt ggtgaacgca ttaataaact agtttttatt ggtatgaaga tggatcaaca
     6781 atgtattaag gaacaacttg agggttgttt aactgattct cttaatgcag ataatttccg
     6841 aaaaatttca ccatttccat ggaaaaagtc taatatgttt agccagatat cgttaatgaa
     6901 atttttaaga aaagctggtg tcaagatagg cgatgtcata attattggca ttttgattat
     6961 tacctctttt attcccttat ttgtattctc tatagaaaag gagaatgaaa caactgaaga
     7021 tgctatcaaa tatgccatcg ttcgaattga tggcaaggaa atggatcgct tcgatttgga
     7081 taagatcaat cataaactcg tgaattatca tcctgcgaag ggacaatata atattgttga
     7141 gattaaagat ggaagaattc gagttaaaga agataatagt cctgatcaaa tcgcggttag
     7201 aactggatgg atctcaaaac ccggtcaaac gagtatttgt ttaccccata agttagttat
     7261 tagtattgag aaaaaagaaa gcacagatta ctacatttac taatctggaa atagatgagg
     7321 aggtttttaa taagaaaaag atattattat taataatagc aggggcttta ttaagtgtgt
     7381 taagcgcttg tggacaagaa aaagaagcgg tgacaacagg cacaagtatc tttaacggga
     7441 cctgcaatgg tttagttaac agtcggttag attacttatt ttggctcttc gtcaatgagt
     7501 gttggtgaag aaattcaata gaataatcga gatactcaca cggctagaca gctatcttgt
     7561 ctagccgtgt tgttgttctg gctttagtca gttgggtagt attcttagtt ttcttaaaat
     7621 attttcctcg atataaaaag attcgttctc ggtagttttg aaagtttcta tagccatagg
     7681 caattcgttt tagtactttg atatggttat tcaaacattc tatagggccg ttggaatagg
     7741 gaacgcgcaa cgctcgtttg atttctgttt ggtatttctt gaaggtcgtg aacactggtt
     7801 ggtagtgctc tggcaactcc ttgtagtctt cttttaggag cgcgacgaat tcaggaacat
     7861 cttgtcttct gataacggaa agaaaatcct ggtagacttc gtagcctcgc tttaaggaat
     7921 catcataaga taatagccga tcgactatct ctgcttctgt cagatgatct cgaaatgacg
     7981 gacgccagta gcgatgctga cctttcaatt cccacgcatt cttttgaagt agtttccagt
     8041 atttctttaa gtgcttcgac cgacgatccc cttttttaaa ggtcttcatt acttgaattc
     8101 ggtggttggt aaaagcccga ccaatgtgtt ggctaatatg aaagcgatcg gcgacgattc
     8161 gggcattcgg aaaacactct ttcaccaatg tgcgataggg tgtatagaaa tctgaaacca
     8221 ctagacgcac ttgttcacgg acttttatag ggtagcgcaa gaaatagtcg cgtaaaggat
     8281 tgagttgccg attttccaca atatcaatca gttgatgggt cttgccgtcc atcatgacaa
     8341 agctcatggc tcccgagaca tttttcactg atttgaattc atcgaaacac accacttctg
     8401 gtaacagctg gctcaaagaa atggttttag gccgaaggct gcgaagtatt cgaatgacag
     8461 tggtcggaga aatgtgcttc atacgtgcga tcaaagacat agaaagaggt tcgcttaata
     8521 gctcaagaat ggcttgtttg actctgcgag caatggagca gcgacgatca ctaactgaat
     8581 cttctgctaa aaaagtcttt ccacagtgtt tacatttgaa gcgctgtttg ttaatccgca
     8641 gaatggtctt gtagttactg acatcattca ataagatgga tacttttttc cagccctatt
     8701 taatgatcgt ttgttcattt tgatagtggc gatgaaagca ttcctccggt tgataggtca
     8761 atgtgccatc aaaaacaaaa catgtttcgt cattgattcg ctctttgcga aaggcatctt
     8821 catgaaagat aagatttagg tctagaatat ctagtaactc tttggtaaac tctgaatagg
     8881 acatcttgaa acactccttt gaatggtttt cgtcgacttt attctaaagg attttaaggt
     8941 gtctgtttgc gtttattaga aaaaagaaaa agtattggca acagaaggcg tcaccaacac
     9001 tagatattat agatccctta ttttcttcgc ttcaaattgt caaggagtca tcataaattt
     9061 tgtatggatt cttttgtttg ggtagtagtc ttctaaataa tcttggatta acaccttcat
     9121 ctctatttta cttttatatt tgttggagtc gattaattct catttgattg atcgatgaaa
     9181 cgcttcaatt actgcattgt caacaggagt cccagtttgg ctcactgaat gtgtgatgtg
     9241 gtggtcgttc aacagctctt gataaataaa agaccgatgc tggctacctc tatcggaatg
     9301 aattaccgta ggcaattctc cttaataaat ttgtaatgga cggacgacaa tctctttttt
     9361 catgtgtgaa tctaccatat agtaacgtag aatacgagtt gctaagtcaa tataggtgga
     9421 caaatagagg cgaccgtcat tacatggaat gtaagtaata tccatcaccc aaacagcatc
     9481 aatttggttc tgttcaaatt atcgattcaa cattttttta ccatttcttt gattgttttt
     9541 tctctaccat gagaagattt gcgtcgatga aatcctcttg tgcataaatt catttcacgc
     9601 ataagaacag ctacaacacg tttattgatt tcgattcctt catcgtaaag tttttgagtg
     9661 atccctggag atccgtatcg atctttgtgt tcgtagaaca ctcgtcttcc tcttttagtc
     9721 gtttattttc ctgttcaaga atgtagaaat ctgctttatc aggtaatctt tttccttgtc
     9781 cagcaaaagc atttttcccg tatcgacgat attctgaaac ccatcgggaa attgttggac
     9841 gagagatatt gtattcttca gcaattttct tggtagttac ttttttctca aggatatcct
     9901 gtgaaatctt tagtttaaat ttacgatcat atgtttttct caatagagtt cattccttcc
     9961 ggtaacgtaa ctgatagtca agaaaagtgt atcatagccc aacttagtat cagggtttgt
    10021 caactaaact gtggaagttg atagataaga gtttttaaca accaaaattc tcttgactat
    10081 ttttgaaatc agacaaattt tgattggaca catagttgaa taaatctacc gtcgttacgg
    10141 aaggtaaatc gcatgctttt caattctaga ggagaagtcc tcgttgaagt accatagccc
    10201 agttctggat tcgacctcct gcccacttca tacgtaaata aagtaatttt aggagtgaga
    10261 ctgtgacaaa agttattttt aagctaaaaa cgaattaaaa a
//
LOCUS       DASJSZ010000091         9928 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.095, whole genome shotgun sequence.
ACCESSION   DASJSZ010000091 DASJSZ010000000
VERSION     DASJSZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.095"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            32..388
                     /locus_tag="U4B03_002342"
     CDS             32..388
                     /locus_tag="U4B03_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HEN2069160.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="U4B03_002343"
     CDS             404..754
                     /locus_tag="U4B03_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HEN2069161.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="U4B03_002344"
     CDS             783..1562
                     /locus_tag="U4B03_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HEN2069162.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="U4B03_002345"
     CDS             1573..2235
                     /locus_tag="U4B03_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HEN2069163.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="U4B03_002346"
     CDS             2254..3360
                     /locus_tag="U4B03_002346"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEN2069164.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="U4B03_002347"
     CDS             3350..4441
                     /locus_tag="U4B03_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HEN2069165.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="U4B03_002348"
     CDS             4454..5194
                     /locus_tag="U4B03_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HEN2069166.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5482..7422
                     /locus_tag="U4B03_002349"
     CDS             5482..7422
                     /locus_tag="U4B03_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HEN2069167.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7509..9431
                     /locus_tag="U4B03_002350"
     CDS             7509..9431
                     /locus_tag="U4B03_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEN2069168.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9711..>9928)
                     /locus_tag="U4B03_002351"
     CDS             complement(9711..>9928)
                     /locus_tag="U4B03_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEN2069169.1"
                     /translation="YFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSEE
                     EILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3241 a         1646 c         2207 g         2834 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaatt acggaaattg ttaatgtaga
     5281 taataattta gatgtacgta agcagaagta cagaaatgta agattttcca atattatttt
     5341 attcaacata ataaatcgta tatcaatttt tttagcgaaa tttctattta ttccaagaat
     5401 ttcagttatt acggaatcat tttgtgctta ataaaaagaa accgttttta tatactataa
     5461 atgtagaaag gcggtaggaa aatgaaaaaa attattgcaa tcacatcctg tccagttggt
     5521 attgcccata catacatggc agcagaaaac ttagagaaag cggggaaagc agttggcgcc
     5581 gaggtaaaag tcgaaacaca tggttccatc ggtattgaaa atgaattgac tgcaagagat
     5641 atcgaagaag cagctggtgt aatcatcgca gcagatacta aaatagataa atcacgattc
     5701 ggcggtaaac ctttgataac cgtaggtgtg caagaaggaa tccaccatgc agatgagttg
     5761 gtgagagata ttttagcagg aaaagcgcca gtctataaaa gtacggaggc aattatttct
     5821 tccgaaaata aaagtgaatc agaaaattta ggtaaaaaaa tctataagag tttgatgaat
     5881 ggcgtttcgt acatggtacc tttcgtagtt acaggagggt tattgatcgc gatttcttta
     5941 acattaggag gaacagctac acctgaaggg atcaagatac cagaaggaac gatttgggca
     6001 acgatgaata gtatcggaag tatcgctatg gggttgatgg ttccaattct atctgcattt
     6061 attgcgcaaa gtattgcaga tcgtccagga cttgttcctg gatttgttgg tgggatgtta
     6121 gcggcaaacg gagcattata tggtagtgat gcgaatgcgg gattcttagg cgggatcatc
     6181 actggatttt tagcaggatt cattgcttta ggaatcaaga aagtgcgagt accgaaagct
     6241 ttacaatcga tcatgccaat tattgttatc ccaattttag gatcattggc agttggtttt
     6301 gtgtttatct atgttattgg agaaccagta gctcttctat ttagttcatt gactcacttt
     6361 ttagcaggga tgcaaggagg aagtgaaatt gttctagcta tgatcttagg agcgatgatt
     6421 gcttttgata tgggggggcc gttcaataaa gtagcctttt tgttcggatc tggcttgatt
     6481 gcagaaggca attattccat tatggggcca attgctgtag ctatctgtat ccctccatta
     6541 gcattggggc tggcatcatt gattttaaaa accaaattta cgaaaacaga acgagaagct
     6601 ggtaaggcgt ccttagcaat gggactgttt gggattacag aaggcgctat tccgtttgct
     6661 tcaacagatc cactacgggt gattccaagc attgtaatcg gggcaatgac aggaagtgtg
     6721 atcgcgatgc tttcaggagt gacagatcat gtagctcatg gcggtccgat cgtagcggtt
     6781 ttaggagcag tggatcacgt agccatgttt tttgtagcag tgattgctgg tattgctgta
     6841 accgtgctaa tgttaagaat ccttaaaccg acattacaac aaactgcggt tgctgcagca
     6901 aatggtgtga cagtagcaga tctatcagag actgaaacaa gtgatgttta tacgaaaact
     6961 gaaacttttg attctatcac ggatattatg tcagatgaag caatcgtttt aggtgaaaca
     7021 gcaacaacaa aagacggagt aatcgaagat ttagtccaac gtttagaaaa aacagcggcg
     7081 atcacagata aaaccagatt taaagaagtc atctatgata gagaacatga aagtacaacg
     7141 ggaatcggta tggggattgc tattccacat ggaaaatcag cttatgttct taaaccaaca
     7201 gtggcatttg ccagaagtca atctggggta gattggcatt ctttagatgg aaaacctgca
     7261 catatgatct ttatgattgc tgtacctgaa aacagccaag gtgatatgca cttgaaaata
     7321 ctgcaacgtc tatcacgtaa attgatggat gatgacttcc gtcaagcact gatggaagcg
     7381 ccagataaaa caacagttca ccaattatta agcgaaatgt aacataagat gaaaaatcga
     7441 ccgtgatgag acataataga tgtgttatca tgatcgattt tttatttaga agggagtttc
     7501 tgcacattat gaatcagcgt cagcaatctt tgatctatca attaagtgag aacaataatt
     7561 atataacaac aaaaaagcta gcagagcatt tccatgtgtc agaaagaacg attcatagcg
     7621 atttgaatac cattgaaact tggttgagag acaaacatct gcctttaatg tttgaaagaa
     7681 aaaaaggtac aggtatcttg ttgaatggtc tgcctcacga aagggaacaa ctgaaacgag
     7741 cgcttaatga agagactcaa acagaaatgg ataaaaaaca gcttcgtcag ttattgattt
     7801 tccatctgct agtagcaaaa gacggattta gtcttgaaga actttcagaa aaactatatg
     7861 ttggaaaacg tacgattcgt aaagaattga cagagttgca aagctttttt gagtatcacc
     7921 atttacagct agtatccaaa acaaaactag gaacttttct taaaggcgat gaacaagaaa
     7981 aaagacagtt gcttgttaag acattacgaa atatgcagaa agaagatcca caaagtccgt
     8041 cgttgaaaga attcttcgct aaagatacat taaaagttat ccaacataca ttacaagatg
     8101 ttttttatga aaaccattta gaacagccga atggactggc tagtgtggag attcacatct
     8161 actttatgct cgagcgaatg aaacagtacc agaaagtgaa attaagtaaa gacgaaaacg
     8221 aagtcgtgga acacacccaa gcacaaaagt taagtagtca aatattagca aaactagcca
     8281 ccatctatcc aattgaattt tctcctgatg aaatcaatta tctggctttg cgtatcgcca
     8341 atctttttac aaattcacaa gcagccacac gttttcaaaa ggaatctagc gcacttgctg
     8401 atcatttgat cgaacaagtg gaacagtttc taggctattc attaaaagaa gatcagctac
     8461 taaaacaaaa tttaaggtct catttatctt caacttattt tcgactccat tatggtttgc
     8521 agatttctaa tccattgacg aaaaatgtgt ttagtacgta cacacaactt ttcttagttt
     8581 tacagttaat tttagaagat tattttgaaa aggaaaattt ttatgttcca caagaggaga
     8641 cagcttattt aacgatccat ttccaagcag cgatcgagcg acacaaacat cagaaaagta
     8701 gaagatatca aacggtttta gttagtgaat attcaaaagc tatggctact tttctcgaag
     8761 cacggctgaa cagagaactt ccagaattaa cgattatgga tctagttgaa tataagccag
     8821 aattaaagag acaagatttt ccctctgttg attttatctt ggctacgtta ccatttaaac
     8881 atataagcat tccagtaatc gagatctcac caatgatcac agaaactgat ctagcctatt
     8941 tgacaaaata tatgctggag catgtgccta tcaagaagaa aaaaacattt gatctagcaa
     9001 gctttactca tccatttctg atctttcctc aacttgaacg gacagaccca gccgatattt
     9061 taaactttat gggaaacgtc ttagtggaac atcattatgt ggagccggaa ttcatcgatt
     9121 ctgtcttgga aagagacagt cactccagta ctagagtagc accatttgta acaatccctc
     9181 acggtaatcc gctgtatgtg aagcattcga tgatttctat tgctacaatg aaagaaccag
     9241 tcctatggca cggggaacag atacgtatcg tactgctatt agcaataaag aaaaatgatt
     9301 tgaaacatgc agagttcaaa aaaatatttt ctgttattca ctatctagaa aagcagccag
     9361 aacaaatggc tcagttgatg caatccaatc atccgctaga aatcttaacg ttgttatcta
     9421 gttatgaata agtagcgatt aaaaaactgc ctttatattt ataataattc tatctattta
     9481 tttggaagta gaaaactaaa tacaaaaaaa gaggatgtgg ccaaagttgt ggcacatcct
     9541 tttattttga gtttcttata attttttata gttgcttacg atgtcttgac tactggtaaa
     9601 cggtgttcaa gagacaattt gtcggcaata aggcacaatt ttgtaaaata tggaaagcta
     9661 tttttttaac attgtttctt agtgcttgaa aaatcatttt gaatacttct ttatacgatc
     9721 gatgtacgtt gatttaagtc taaaactttt cttttgattc cttctctgac ttttctgaat
     9781 tccgttaata tttcttcttc gctcccgaca gcttgtgcag gatcttttag gtcccagtga
     9841 aggtgttcgt gattgtttgg aacggcaggg cacttgtctt tggcatcacc acaaagtgta
     9901 atgatcaagt ctgcctgagt aaagtaat
//
LOCUS       DASJSZ010000092         9628 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.096, whole genome shotgun sequence.
ACCESSION   DASJSZ010000092 DASJSZ010000000
VERSION     DASJSZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.096"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..656
                     /locus_tag="U4B03_002352"
     CDS             33..656
                     /locus_tag="U4B03_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069170.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="U4B03_002353"
     CDS             1106..2155
                     /locus_tag="U4B03_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2069171.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="U4B03_002354"
     CDS             2152..3033
                     /locus_tag="U4B03_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2069172.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="U4B03_002355"
     CDS             3036..3806
                     /locus_tag="U4B03_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2069173.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="U4B03_002356"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="U4B03_002356"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEN2069174.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="U4B03_002357"
     CDS             5117..6280
                     /locus_tag="U4B03_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2069175.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="U4B03_002358"
     CDS             6397..6807
                     /locus_tag="U4B03_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2069176.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="U4B03_002359"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="U4B03_002359"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2069177.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..>9628
                     /locus_tag="U4B03_002360"
     CDS             9380..>9628
                     /locus_tag="U4B03_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069178.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLP"
BASE COUNT         3344 a         1635 c         2074 g         2575 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctacca
//
LOCUS       DASJSZ010000093         9555 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.097, whole genome shotgun sequence.
ACCESSION   DASJSZ010000093 DASJSZ010000000
VERSION     DASJSZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.097"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            85..987
                     /gene="gnd"
                     /locus_tag="U4B03_002361"
     CDS             85..987
                     /gene="gnd"
                     /locus_tag="U4B03_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEN2069179.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            989..1990
                     /locus_tag="U4B03_002362"
     CDS             989..1990
                     /locus_tag="U4B03_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEN2069180.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1987..2271
                     /locus_tag="U4B03_002363"
     CDS             1987..2271
                     /locus_tag="U4B03_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEN2069181.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2304..3101
                     /locus_tag="U4B03_002364"
     CDS             2304..3101
                     /locus_tag="U4B03_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2069182.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3115..3948
                     /locus_tag="U4B03_002365"
     CDS             3115..3948
                     /locus_tag="U4B03_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2069183.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3966..4823
                     /locus_tag="U4B03_002366"
     CDS             3966..4823
                     /locus_tag="U4B03_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2069184.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4845..5354
                     /locus_tag="U4B03_002367"
     CDS             4845..5354
                     /locus_tag="U4B03_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2069185.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5391..5792
                     /locus_tag="U4B03_002368"
     CDS             5391..5792
                     /locus_tag="U4B03_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069186.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5904..6257
                     /locus_tag="U4B03_002369"
     CDS             5904..6257
                     /locus_tag="U4B03_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069187.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6291..6734
                     /locus_tag="U4B03_002370"
     CDS             6291..6734
                     /locus_tag="U4B03_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2069188.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6751..7836
                     /gene="uxuA"
                     /locus_tag="U4B03_002371"
     CDS             6751..7836
                     /gene="uxuA"
                     /locus_tag="U4B03_002371"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2069189.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7829..8542
                     /locus_tag="U4B03_002372"
     CDS             7829..8542
                     /locus_tag="U4B03_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2069190.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8749..8898)
                     /locus_tag="U4B03_002373"
     CDS             complement(8749..8898)
                     /locus_tag="U4B03_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069191.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9294..9494
                     /locus_tag="U4B03_002374"
     CDS             9294..9494
                     /locus_tag="U4B03_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2069192.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3217 a         1499 c         2114 g         2725 t
ORIGIN      
        1 aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag
       61 ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga
      121 ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca
      181 agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa
      241 tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata
      301 accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa
      361 ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa
      421 atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc
      481 ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta
      541 gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg
      601 atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta
      661 caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg
      721 gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta
      781 agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg
      841 ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca
      901 caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga
      961 catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc
     1021 cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt
     1081 tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg
     1141 attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa
     1201 agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc
     1261 agcaaaagaa caccagacaa tcgtatctat catacctgct ttaattgaac gaaatgctgt
     1321 agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc
     1381 aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa
     1441 caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg
     1501 taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga
     1561 aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg
     1621 tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca
     1681 gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc
     1741 gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga
     1801 agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca
     1861 tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt
     1921 cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat
     1981 aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat
     2041 ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa
     2101 agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt
     2161 ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct
     2221 gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg
     2281 gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg
     2341 gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata
     2401 aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta
     2461 taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag
     2521 atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg
     2581 aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc
     2641 attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc
     2701 tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa
     2761 gaaatgcggc agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc
     2821 gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc
     2881 gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc
     2941 tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg gctgaattta
     3001 ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac
     3061 agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa
     3121 acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc
     3181 tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc
     3241 gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg
     3301 atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca
     3361 gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt
     3421 gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact
     3481 tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga
     3541 tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt
     3601 gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc
     3661 gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact
     3721 gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt
     3781 gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc
     3841 gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac
     3901 aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat
     3961 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
     4021 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
     4081 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
     4141 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
     4201 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
     4261 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
     4321 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
     4381 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
     4441 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
     4501 tcactatggg ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg
     4561 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
     4621 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
     4681 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
     4741 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
     4801 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
     4861 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
     4921 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     4981 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     5041 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     5101 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     5161 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     5221 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     5281 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     5341 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     5401 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     5461 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     5521 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     5581 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     5641 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     5701 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     5761 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     5821 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     5881 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     5941 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     6001 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     6061 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     6121 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     6181 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     6241 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     6301 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     6361 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     6421 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     6481 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     6541 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     6601 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     6661 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     6721 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     6781 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     6841 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     6901 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     6961 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     7021 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     7081 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     7141 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     7201 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     7261 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     7321 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     7381 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     7441 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     7501 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     7561 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     7621 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     7681 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     7741 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     7801 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     7861 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     7921 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     7981 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     8041 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     8101 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     8161 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     8221 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     8281 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     8341 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     8401 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     8461 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     8521 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     8581 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     8641 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
     8701 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt
     8761 ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc
     8821 tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc
     8881 tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa
     8941 cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta
     9001 agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa
     9061 tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta
     9121 tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct
     9181 attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa
     9241 tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata
     9301 acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg
     9361 gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag
     9421 aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca
     9481 ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt
     9541 ttgtacgaaa aaatt
//
LOCUS       DASJSZ010000094         9537 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.098, whole genome shotgun sequence.
ACCESSION   DASJSZ010000094 DASJSZ010000000
VERSION     DASJSZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.098"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..793
                     /locus_tag="U4B03_002375"
     CDS             <1..793
                     /locus_tag="U4B03_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2069193.1"
                     /translation="NLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYN
                     PVNGVSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGL
                     RYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIE
                     ENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLT
                     ELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(875..2647)
                     /locus_tag="U4B03_002376"
     CDS             complement(875..2647)
                     /locus_tag="U4B03_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2069194.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(2650..4362)
                     /locus_tag="U4B03_002377"
     CDS             complement(2650..4362)
                     /locus_tag="U4B03_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2069195.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4477..5145
                     /locus_tag="U4B03_002378"
     CDS             4477..5145
                     /locus_tag="U4B03_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEN2069196.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(5212..6000)
                     /locus_tag="U4B03_002379"
     CDS             complement(5212..6000)
                     /locus_tag="U4B03_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEN2069197.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6083..7555)
                     /locus_tag="U4B03_002380"
     CDS             complement(6083..7555)
                     /locus_tag="U4B03_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2069198.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(7685..8878)
                     /locus_tag="U4B03_002381"
     CDS             complement(7685..8878)
                     /locus_tag="U4B03_002381"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEN2069199.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(8876..8955)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(9144..9215)
                     /locus_tag="U4B03_002382"
     tRNA            complement(9144..9215)
                     /locus_tag="U4B03_002382"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9180..9182),aa:Glu,seq:ttc)
     gene            complement(9227..9300)
                     /locus_tag="U4B03_002383"
     tRNA            complement(9227..9300)
                     /locus_tag="U4B03_002383"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9264..9266),aa:Ile,seq:gat)
     gene            complement(9313..9383)
                     /locus_tag="U4B03_002384"
     tRNA            complement(9313..9383)
                     /locus_tag="U4B03_002384"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9349..9351),aa:Gly,seq:tcc)
     gene            complement(9388..9460)
                     /locus_tag="U4B03_002385"
     tRNA            complement(9388..9460)
                     /locus_tag="U4B03_002385"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9425..9427),aa:Phe,seq:gaa)
     gene            complement(9464..9536)
                     /locus_tag="U4B03_002386"
     tRNA            complement(9464..9536)
                     /locus_tag="U4B03_002386"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9501..9503),aa:Asp,seq:gtc)
BASE COUNT         3020 a         2054 c         1590 g         2873 t
ORIGIN      
        1 taatcttata caagatatgc tagattacta ctattttgaa aaaaatttat catataatta
       61 tgtaatacat ttaaaaactt ttacaggaat gatttttaaa ttcgccaacc gtcgatatgg
      121 attaaaatat aatcccgtaa atggggtttc tttagcaaga aaacctaaga caaacgaaga
      181 aataaaaaaa gaaaaagatg gttatttatc aaaagaagaa gttcaaatga ttgctagtaa
      241 acaaaaaagt tcacatcaac agtcacgtta ttctttaatt actaaatttt tgtttttaac
      301 tggactacgt tatggtgaat tgatatcttt gactgaatct gattatgatg gtaataaaat
      361 tacggtttct ggtacttatg attacgagtt aaaaattaaa acaacaacta aaaatacagg
      421 ttcttataga accattgaat tatcaagtaa tgctaaagaa atcatagatc aattaattga
      481 agaaaacaaa ttaattaaga ataataaaga taaatacatt ttcatctcta aaaatggtaa
      541 cccgatatct attcaagcat ataatcaatc tttaagagca gtatccaaag aattaaacct
      601 agataaaaaa gtttcttctc acatgttaag gcattcccac atttctctac ttacagaatt
      661 aggaatccca ttaaaagcca ttatggatcg tgtaggacat gaagattcaa aaacaacatt
      721 aaagatttat actcatacaa ctaaaaatat gcaaaatcaa ctagtagaaa aactaggaaa
      781 aatagaaatt taaattgccc cttttctgcc ccttataaaa acaaacaaca taaaatatat
      841 aaaaagaacc ccgttataac gaggttcttt taatttaact ttcaaataca aattgattat
      901 gataaagttc cgcataaaat ccattttctg ctaataactc gtgatgactg ccttcttcaa
      961 tgacttcccc atcacgcaag acaacgattc tatctgcatt caagattgtt ttcagccggt
     1021 gagcaatcac aaagctggtt cttcctttga ttgcttcatc cattgctttt tggatccttg
     1081 cttctgttac cgtgtctaca ttacttgttg cttcatccaa aatcaataaa gatggatttg
     1141 tgatgatcgt tcgagcaata ctgatcaact gcttttgacc tgtactgaat aagttatttt
     1201 cttcggatat ctctgtatca taaccttttt ctaacgtcat gatgaagtca tgaatattcg
     1261 cttgttttgc tgcattgatg acttcttcat ctgaagcgtc cggtttgccg aatgtgatat
     1321 tgtctttgat cgttccggag aacaatacag attcttgcaa aacgattcct acatgtctac
     1381 gtaatgcatc aagatcataa tctcggatat ctattccatc gatgactact gaaccatcat
     1441 tgacatcgta aaaacggttt agcagattca tgatcgtcgt tttccccgat cctgttggac
     1501 caacaagagc aaccatttct cctttgtcca cattgatcga gacatctttc aagactagtt
     1561 tttctggaga ataaccaaaa tctacgtgct gcaacgcaag acctttttca acaccatcaa
     1621 atgctttaca gttttctggt ttgacttcat ctttttcatc aaatatttca ttcaatcgtc
     1681 ttgcccctgt aatcgctaat tggatcatgc tgtatccaga tgaaatctgc atcagtggtt
     1741 ggtaatattg ttgtgaatac tggacgaatg tcacaactaa gcctaatgcc accgaacgtt
     1801 ccaaatcacc atttaatgct aaccagccgc cgaaaaagat aactatcgct gtattgacca
     1861 gtgacattcc ttgcatcatc ggaaatagta gaccagaata agcttgtcct ttgaaggttg
     1921 cttcgcgaac actttgatta tgctctaaga atccttcaat cgtttcttct tgtaagccat
     1981 ttgtgatgat gacacgttgt ccgctgattt tttcatccat ataaccattt agtttcccca
     2041 cttcatcttg ctgaagatcc acatatttcc gtgctttgcg gataatcaag acagctatca
     2101 aaacggctac tggagtggaa gcaattgtag cccaagctaa ctctacattt tggcggaaca
     2161 tcatgatcaa gatcccgaca agtaaaacag cattcgtgat gacttgaagc aaagcttggt
     2221 tcaaactatt ttgaatatta tctaagtcac tggtaaaacg acttaaaatt tcaccgtctt
     2281 gatgagaatc aaagaaacgg atcgtcaatt tttccaattt gttgaataga ccgatacgca
     2341 ttcggttcgt tgacttccct actacttgtg tgaacaaaat actatagata aagttggctg
     2401 cactagctag tacataaaac agcaagagtt tccatattac agaaacaaat ttgcttttat
     2461 cgtctacgcc ttgcatcaag cctcctacgt aattagctaa ttcttgaaaa gcttccccga
     2521 tgtattgagg tgcctttact tgtagatagg tagcaatgat cactgtgata aagataaaga
     2581 aaaaggagag tttgtaccgc ttcaaatagt gatagaaaaa tttacttgct tttattagat
     2641 cagtcatact tactcctccg ttcctttttg agtttcaaag atttcctgat agacacggtt
     2701 tccagctacc aattcctcat gggttccttc gccgactaat ctgccttcat ccaatactaa
     2761 gatgcggtct gctttgacaa cagaagcaat tttttgtgcg atgacaatcg tcgttgttcc
     2821 cttcaaatct cgatcgagtg cttctcggac cagcttttca gagcgagcat ccagtgcact
     2881 cgtactgtca tctaggatca agatctttgg attaccgata acgccacgag taatggataa
     2941 acgttgcttt tgaccaccag aaaagttact gctacgttct tctattggtg cttcgtaacg
     3001 atcagataac ttttcgatga attcttttgc ttgagcaata gaagaagcat gttccatctc
     3061 tttttctgtt gcattctttt tcccttggcg caagttttga gcaatcgttc ctgagaataa
     3121 aattgctttt tgcaaaacaa aggaaaccgt ttgtcttaaa ctcttttcat ttacttgacg
     3181 aagatctaca ccacctactt caactttgcc ttctgtcgga tcaaatagac gagggatcaa
     3241 ttgtgctagt gttgacttcc cggcaccagt cgccccgacg ataccaacca tttctccagg
     3301 tttgatagaa aagctgatat ctttaagcgt atctgtatcc tcaccaggat aacgaaaaga
     3361 aacatggtca aaagtgacag aacccgtcaa ctcttgttct ggaacgtcta aataagtaag
     3421 atctggttca gcatctaaaa tttccgcaat ccgcttcagc gatacggctg ctcttgaagt
     3481 catcatcatc atcattccgc caataatgat tgccatcata atctgcatca agtagttcat
     3541 gaatgaagca attccaccaa ttaacgtagg atcatcttta acaagatcgc tcacaaaaaa
     3601 gatcgcccca accacagcca aattggcagc cagcatgaag gacgggatca tcacagaaaa
     3661 taatgtaccg actatgatgt tgtgttttgt taagtcctca cttactttgc tgaatgactt
     3721 caattggttt tcttcttgta caaaagattt cactacacga atccctagta agttttcttt
     3781 tgcaatgcta ttcacacgat caatcagttt ttgaatgatc atgaagtgct tgcccatcct
     3841 tgtaaatgaa agtgccgtaa tcagaaatac cgtaacgata agcgctacga tgatccacca
     3901 taattgcggc atcgtgatca tagctaaaat gaaggcacca ataaataaga atggaattcg
     3961 aaaaagtgat tgcaaagaaa tcatcactaa attttggatt tgcgtgatat catttgttag
     4021 acgaacaaca agatttccag cagagaactg ttcgatattt ccaaatgaaa atgtctggat
     4081 ctttcgaaat gcttcttctc ggatatctgc acttacgcct tgagctactt ttgcagagaa
     4141 aatcgtgttc gttacacctg cggctaatcc tagcaaagcc agactgatca ggtaaatacc
     4201 aattgttttc atttcatcat tatcttctgt aataatagct tccaatactt gctgtagtaa
     4261 cttaggttgc cataatgctg ctgcaaccat gactgctacg gacagcaagg aaataaaaac
     4321 agctacttta tattttttcg catgtcgtaa gattaatttc attcgttgat tacacccctt
     4381 tgtttaaatt gacaggacta atttaacact gtaaaattag accgactaac aatttaccat
     4441 gtttaaaaag ataatgcaat acacgaggtt gataaaatgg aaataaagct ttctcaagca
     4501 atcatcattc aggccgcctt tgatatcctg gctgaaacac aagctctttc gcagctttcc
     4561 atgcgaaaca tcgcaaacag aattcatgtt caagcacctg ctttatattg gtatttcaaa
     4621 aacaaacaac agctcttgca aataatggct gaaacaatgg aagaagaatt agcgcttcca
     4681 gatccaactt tgccttggaa tgaacagttg ctgcagtata tggaaaatta ctatgacctt
     4741 tatacacgtt tcccttgtgg ggcagaatta gaaatcaata ctgtgcccgc ttttccgagt
     4801 cgattagaac atttggagca aatgatccaa ttacttgttc gtgaaggatt ttcagtcacc
     4861 caaagtcatc aggccattgc ctctctccac aatttattga ttggacagtt aatggatcag
     4921 cagcatgaac agcagcttcg acaaaaaatt atggacggaa atgaattttt gaaagaaaca
     4981 gttctattta tgcgtaatta tgtagttgaa aatcaattag agggatttaa agaaagtatg
     5041 cagtcccatg cagaattgaa tgaaaaagca ctctttctaa cgagtgcaca aacctatatt
     5101 gaaggcttat gccaacaaaa taaaagaaga gaaaacgagc agtaaaaaag ctcctataga
     5161 gatcactatg tttgtgatct ctataggagc tatcttttta agactctttt tttatttttc
     5221 ttccattagc cacgccaatg cacgttgaat ccattggtcc cagaagatcc agttatgatc
     5281 gccaggtcct tgttcaaagg tgaccgccaa gccttgttcc tgcatagcag ataccatttc
     5341 ctgatttgcc tgataaagaa aatcagattc cccacaagcc atataaaatc ttggaactgg
     5401 ttttccgctt tttactagtt ctttcaataa gtattgcgga tcattttttg aaccttcgat
     5461 ttgatccaat ggtccaaaga tcccttccca aaaagatgct ttacgaacca tcaaaagatc
     5521 atccattcga tttcctacag aaacagcgcc tgaaagagag attactgctg cgaaattttc
     5581 tgattttgca aggcctagtt ttaacgcgcc atatccgccc atcgataatc cagcggcata
     5641 tgttttttct ctttttcttg ttaattgcgg gaataattca tgacaaatct caggtagctc
     5701 ttctgcaatg aatgtccaat acttcatgtc ataaactgta tctgtgtacc agccaagatc
     5761 agtagatggc atgatgacag ctaatccgta gtttgctaca taacgttcaa tcgacgtacg
     5821 tctttcccat acactatgat tgccacccat tccatgaagt aaatataaaa caggtatatc
     5881 cgttgtctct tgctctgaac tggtgccaat cacctttttt gttgtttgcg gtagtatcac
     5941 atttagattg acttccattt ctaacacatt cgaataaata tttgcttgaa taaatgccat
     6001 attttcgcct tccttagtaa gaagttattt ttgaataact ttaaatttga atgattcggc
     6061 tacgtacata tcttaaccga aactactgtt ttattttacc acttttttca tgaacgaaaa
     6121 gcgttaagac caaagcaatg atcacgagta acaagccaac tagataaacg gaatgtaacc
     6181 catcataaag aatttttctt aatggtaaca aaatatccgc atcaatatat ttagccgttt
     6241 gtggattcac cagtttattc atgatatctg catcatttgt taaatttgct gctgcaagtt
     6301 ctcgtttagt taccccattt atcaataaac caaaaataga aaccatgact gtttgaccaa
     6361 ttgttcgaac taacgtatta aaagaagttg ctacaccaag ctggtcacga gatacactat
     6421 tttgtgcact gacagtcgag gtagtcatgg tcacacctaa gcctataccg atgaccgttg
     6481 atatagcaaa aaaccataaa tagttacttc tgaaaggcat aagatacaac caagcgcccc
     6541 caatagctgc gattagtagt ccaattctca agacccaatg agttttcatt tgagtcatcc
     6601 acttgcttgc aaggaaagaa ccgaccatcc agactagtga taatggtgct aataccagcc
     6661 caccaattgc tgcattcagc cctaatacac cttgcatcca catagggata taaacatcga
     6721 ttcccatcaa aaaaccacta gccaatgctg cagccaagtt gaccacaaca aatagtggat
     6781 ttttgaataa atgcatcatg atgacaggat ctttagctct tttttcggca aaaacaaaaa
     6841 tacctaacat tacgatacta cctagcagca aagcagaaac aagcggactc attccttcgt
     6901 ctcctaataa ttggaaccct aacagcaatg agacaagcat ggccatcaaa ctaaaactac
     6961 caaaaatatc cattggttta gactcatgta tgatttttgg ttcatttaaa tatagccaaa
     7021 tcaaaataat taatagaatt ccgatcggca cattgataaa aaagatccag tgccagctga
     7081 tcgtttcaac gatgaatcct cccgcaagcg gtccaaaaac actggcaatc ccccaggctg
     7141 aactattcaa acctaaaact tttgctcgct tttcaataga ataaagatcg ccgatgatcg
     7201 tcaaagctac tggcatcatt gccccagcac ccatcccttg aatcgcacga gcagcaatca
     7261 acccaatcat gctttgagat aaaccgcaaa ataacgaacc gataataaac aataatgtcc
     7321 caaaaataaa gattggtttc cgtcccactt tatccgcaag ttttccgtaa atcggagtaa
     7381 gcatagcgtt tgtcagcaaa taaatcgaaa atacccagtt catgatttcc attccatgta
     7441 atgaaccgac aatcgttggc atagcggttg ttacgattgt accttcaata gccgtcatga
     7501 acgttgcaat gaaaatactc actgtgacca aagtaacatt tgtttctttt ttcatcttat
     7561 tcctcccttt cattatgaca ggatctaaaa atagataaat aattactatc tttttcaaga
     7621 ggttaactgc cataaaaaaa gctggaacaa ccaccacttg gcgatcgttc cagctgaata
     7681 taacttattt tgctaaactt gctttcaacg catctacttt gtccgtatgt tcccaaggca
     7741 aatctacatc tgtacgacca aaatgaccgt aagcagctgt ttgtttgtaa atcggacgac
     7801 gcaaatcaag catctcgatg attcctgctg gccgaagatc gaagttttct ctcactgcag
     7861 caatcagttc ttcttccgga accgttccag taccgaaagt attgattgag atcgaaacag
     7921 gttgagcaac accaatcgca taagctagct gtacttctgc tttgcgagca agccctgctg
     7981 caacaatgtt tttagcaata taacgtgcag catagctggc agaacggtca acttttgtcg
     8041 catctttacc agaaaaagca ccgccaccat gacgagcata accgccatac gtatcaacaa
     8101 tgatttttct tcctgttaat ccggcatccc cttgaggacc accaatgaca aatcggccag
     8161 tcggattgat ataatatttc gtttgatcat ctaataattc agctggaata acttccttca
     8221 ctactttttc gatgacatca tgacgaattg tttcattatc tactgcatca tcatgctgtg
     8281 ttgaaataac gattgtatct acgcgttccg gttgtccttg atcatcatat tcaaccgtta
     8341 cttgagattt tgcatctgga cgtaaatacg ttaattcatt tgatttacgc aaatctgcca
     8401 agcgtcgtac cagacgatga ctcaaagcga ttggtaaagg catcaattct ggtgtttcat
     8461 ctacagcaaa cccaaacatt aaaccttggt caccagcacc aatctcatct aatacgtctt
     8521 ttttatcttc atcccggatc tcaagtgctt catcaactcc ttgagcaata tcaggagatt
     8581 gttcatcaat cgcaaccaat acagctgctg tatctccatc aaatccgaat tttgcacgtg
     8641 tataaccaat ttcttttatt gtttcccgta cgactttttg aatatctaca tatgccgtcg
     8701 tagatatttc tccaaatact aaaacaagac cagttgttac agacgtttca cacgctactc
     8761 gtgctgttgg atcttgtttt aaaattgcat ccaagattgc atcactgatt tggtcagcaa
     8821 ttttatctgg atgtccttca gaaacagatt ctgatgtaaa taagtgtctt tctaccatta
     8881 taatgattcc ccctagttaa attcggttac aaggcatctc cgtcctgcgg atcctttcgg
     8941 gaacttgcaa cgaaccaagt atagcagata aaacagtttt ctgctcgtat tttttcattt
     9001 ttttgcttaa aaaatataaa aatattttac attagaactg tccaaaaaag cttagcacaa
     9061 tcatccataa agataactct gctaagcttt ttaaaatcat tctttcagat aaaaagaaaa
     9121 agatactcaa aaagtatctt ccatgacccg tacgggattc gaacccgtgt taccgccgtg
     9181 aaagggcggt gtcttaaccg cttgaccaac gggccaataa aataaatggg cctaaatgga
     9241 ctcgaaccat cgacctcacg cttatcaggc gtgcgctcta accagctgag ctataggccc
     9301 ataaaaacta aaagcgggtg acgagaatcg aactcgcgac aacagcttgg aaggctgtgg
     9361 ttttaccact aaactacacc cgcataatgg cgcgggacag aatcgaactg ccgacacatg
     9421 gagcttcaat ccattgctct accaactgag ctaccgagcc aaacggttcc gacgggaatc
     9481 gaacccgcga tctcctgcgt gacaggcagg catgttaacc cctacaccac ggaacca
//
LOCUS       DASJSZ010000095         9256 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.099, whole genome shotgun sequence.
ACCESSION   DASJSZ010000095 DASJSZ010000000
VERSION     DASJSZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.099"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..655)
                     /locus_tag="U4B03_002387"
     CDS             complement(<1..655)
                     /locus_tag="U4B03_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2069200.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     "
     gene            789..1076
                     /locus_tag="U4B03_002388"
     CDS             789..1076
                     /locus_tag="U4B03_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069201.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1492..3030
                     /locus_tag="U4B03_002389"
     CDS             1492..3030
                     /locus_tag="U4B03_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEN2069202.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3027..3992
                     /locus_tag="U4B03_002390"
     CDS             3027..3992
                     /locus_tag="U4B03_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2069203.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3979..5301
                     /locus_tag="U4B03_002391"
     CDS             3979..5301
                     /locus_tag="U4B03_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2069204.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5364..5468
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5542..6285
                     /locus_tag="U4B03_002392"
     CDS             5542..6285
                     /locus_tag="U4B03_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2069205.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6300..7292
                     /locus_tag="U4B03_002393"
     CDS             6300..7292
                     /locus_tag="U4B03_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2069206.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7304..8041
                     /locus_tag="U4B03_002394"
     CDS             7304..8041
                     /locus_tag="U4B03_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2069207.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8031..8753
                     /locus_tag="U4B03_002395"
     CDS             8031..8753
                     /locus_tag="U4B03_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN2069208.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8837..>9256)
                     /locus_tag="U4B03_002396"
     CDS             complement(8837..>9256)
                     /locus_tag="U4B03_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN2069209.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3026 a         1599 c         1959 g         2672 t
ORIGIN      
        1 gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca
       61 aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc
      121 taagtaatgt atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac
      181 caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt
      241 gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa
      301 tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta
      361 tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc
      421 ggagaaaagc gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa
      481 gcagtgaagt gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag
      541 taaccccacc atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga
      601 ttcctgcgcc taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta
      661 gcctcttttc ttttgaaaat tttcagcaac ttctttatta tactgactta tactcttact
      721 tcaagaggaa aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga
      781 gtggaaaaat gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag
      841 agtatcaagg agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga
      901 taaataaagc aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga
      961 aagacatctt tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag
     1021 aagaactcat tcctctttat ggagtgaaag agatagaaaa taaagtggtg atataagtaa
     1081 aaataagaaa acaagacgtt tatgatgtca aaacaaccat tttttacatt tttggcaaaa
     1141 aagtgtcatg atacagtata atattcgttg aacaggccaa taaaggtgtt ctcccccacg
     1201 agatttctac tttattggct gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag
     1261 atatagctga ttccaagtga caatttcgta tagattttta tggtattcgt agtgaaaaaa
     1321 cgagaaccaa aaagttgatc cgactttgtt gcttggattc tttttttgtc agcatattta
     1381 gaggtatttt tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg
     1441 gaaaaaggaa agaaatgttt atacttaaag aataacagaa aggaaggtcg catgaataat
     1501 tttatacaaa cattaagtga aagaaaaggc gagttgctgg aagctgcatt tcaacatctg
     1561 tccatttctc tcatttcttt gctgattgca gcactgattg ctattccttt agcgatttgg
     1621 gcagccaatc ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg
     1681 attccttcac ttgccttatt aggtttactt atcccatttg tagggatcgg aactgttcca
     1741 gcattgattg cacttgttat ttacgctttg cttccgattt tccaaaacac gtatattggt
     1801 ctagctgaaa ttgacccgtc tattgaagaa gcagcggtag catttggtat gtccagaatg
     1861 agacgcttgc tgaaagtaga gcttcccatc gcattacctg tcattatctc aggtatccga
     1921 actgccttag ttttaatcat cggtacggct actttggcag cattgatcgg agcaggtgga
     1981 ttagggacat ttattttgtt aggtattgac cgaaatacac ctgttttgac attgattggt
     2041 gcgatcagtt ctgccttgct tgcaattata ttcagcggac tgattcgctg gttgcagcat
     2101 aagaaacctc gatattccat cattacttta gcagcagtgg tgtgtttatt tggcggcgtt
     2161 tctttatatc agtctgcctt gtttaaaaat gaaaccatca cgattgctgg aaaattagga
     2221 gcggaacctg aaatcctgat ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca
     2281 gaagttgaat taaaaccgaa ttttggcaag acgagttttt tgttcaatgc actggatcaa
     2341 ggtcaaatcg atatctatcc tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta
     2401 cctgaagcaa tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga agcagaagaa
     2461 ttattgaata agcaattcga tatgacatta ttaaaaccaa tggcttacca aaatacgtat
     2521 gccttggcag tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg
     2581 gcagctatct ctgatcagat caaagcggga ttcacactgg aatttattga tcgttcagat
     2641 ggatataaag gaatccaaga agcatatggc ttgaattttt caagtgtaca aagcatggag
     2701 ccaagtttgc gttaccaagc aatcaataat ggagatgtca atattgtgga tgcctattcc
     2761 acagatagcc agctcaagga gtacgatcta gtgactctag aagatgatcg agggttattc
     2821 ccagcttatc aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc
     2881 gttaaagcac tgaataaatt aagcggaaaa ataacagagg atcaaatgat tgatatgaat
     2941 taccaagtca atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt cctgaaaaat
     3001 gaaggattag taaaggagga agatcagtga ctaaattaat cgagttcaaa catgtgcaaa
     3061 aaaagtatga tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa
     3121 tctttgtttt ggttggtcct tccggtagtg gaaaaacaac tactttgaaa atgatcaatg
     3181 gattatctaa accatcagca ggagacatct acttcaaagg aaaatcgtta aatgaatata
     3241 atctgcagaa aatgcggtgg aatatgggat atgtgctgca gcaaatcgct ttgttcccga
     3301 ccatgacagt caagcaaaat attgaagtca tccctgaaat gttaggttgg gaaaagcaga
     3361 aaagagcaga ccgtgtagat gaacttcttc aaaaagttgg cttatctcct gatatttatc
     3421 gagatcgtat gcctagagag ttgtctggag gagaacagca acggatcggg atcatccgag
     3481 ctattgctgc aagcccagat gtgattttga tggacgagcc atttagcgca ttagatccga
     3541 tttcaagaaa ctcattacaa gaattggttt tgtccttaca cgaagaattg ggcacgacca
     3601 tcgttttcgt tacgcacaat atggaagaag caatcaagct aggtgatcgt attgcattta
     3661 tgaaggatgg agaaattatc caatgtgaca caccagagca gttattgatg aatcctaaaa
     3721 acgactacgt tcggcatttc tttgatgaac cgaaacagac gaaagagtgg cgagtagaag
     3781 atttagtcgt caatggatat ttcttaaatg aaataccaga aaatgctcgt caacaagttt
     3841 gctttgatac gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca agcattgtga
     3901 tagtagaaaa gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt gcttttttaa
     3961 gtcgagagga ggaaggaaat gaaaacatat gatgcaatca tcatcggttc aggtgtgagc
     4021 ggcttaagtg ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa
     4081 gatctatggg gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt
     4141 gcagtagagg cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca
     4201 actgccaact gggaagaact tcaaaaattc aaacgaacat ttacggatcc agtcccagaa
     4261 agccgaaaaa aacaattagc agaagctgaa atcgatcatc tttcaggaac cgctcgtttt
     4321 ttggatgatt catctattga agtcaacgaa gaagtcttcc atgcggatta cttagttttg
     4381 gcaaccgggc aaagacctac gatcttacca gtggagggaa aggaatactt gaaaactagt
     4441 gcagactttt tatcattgcc tgtactgcct aaagaaatca tttttatcgg tggtggttat
     4501 attgcttttg agctagctac gattgccaat gctgcaggga gcaaagtaac cattgtccat
     4561 cataatcaac ggccattaaa agaatttgag gcgtctttgg tagaggaagc cgtccatcaa
     4621 atggaagcat ctggcattca gtttgctttt ggtgttgaaa cccaaaagat catttcagaa
     4681 ggtactcgct atcgtttagt cggaaaagag acggaattag tagcagatat gattttttgt
     4741 gcgactggga gacagccgaa tacagaatct ttagcacttg agcaagcgaa tatcgtcttt
     4801 gataaacacg gaattgcagt caatgattat ctgcaaacga gcaatccaaa aatttttgcg
     4861 tgtggagata ttgtttcaag gaaaacccca aaattgacac cagtcgccac ttttgaagga
     4921 aattatgtag ctaagcggat aacggatgca acaagcgaac ctatcaaata tccgattata
     4981 cctacgatcg tctacgccag tcctaaactt gctgaagtag gagttacaaa aagtcacgcc
     5041 tcttcgtcag atcaagtggt agagatggat cttaccagtt ggttcaccta tcatcgtgtc
     5101 aatgaaccag ttgctaaggc tgaattgacc tttgaccagc aaaattattt gattggtgct
     5161 gcagtgatta gcgagcaagc agacgaattg atcgatgatt tgacactcgt catcaatcaa
     5221 aaattgacta aaaaagagtt ggatagctat atcatggggt atccgacatt ggcaagtgat
     5281 ctctcctatt tattgaaata atcagcatga taactaaagc gcttgttgct ttttccttct
     5341 tgtgctacac tttaaaaaat gaatacctgt ttctggggat gtcgtagacg acatgagagt
     5401 aagttgtgaa cttagaccca ttgaacctgt cagttaatac tggtgaaggg agaaacatag
     5461 cagacgcttg aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg ttattcatat
     5521 tagaagaaag gaggtaacaa aatgaagaaa aatctttatc cagcagtcag tatggcattc
     5581 attttaatta tttggcagat tgttgtcata gtttggaaaa tacctgcctt tattttaccc
     5641 ggacctgctg ctgtattttc tgcattagcg gcagatgcac aagtattact ttcccattct
     5701 gtcacgacat taaatgaagc agtgcttggt ttggtaatag ctagcgttct ttcttttatt
     5761 actgccttgg caatggatta ttggaagttt ttagaaatgt ctttttatcc gttactagtg
     5821 atttcacaaa tgttgccaat catggtgtta gggccattac ttaccctatg gtttggtttc
     5881 ggtatgcttc caaaagtgat ccttgttgtc ttgataagct attttccgat cgtagtttct
     5941 ttttcggata cattgaggaa agtatcaaaa gagcaaatca tctttttgaa aacgatgggg
     6001 gcagatacag ggaagattta tcgaatatat aaaataccag cgggtttatc cggcttcttt
     6061 tccggtttaa aagtggctgc tacttattgt gtcagcggag cagtcgttgg agaatggcta
     6121 agcgctcaag caggcttagg gtattacatg atacgcgtaa aaaacagcta ccagatcgat
     6181 aaagtttttg ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt
     6241 tcattattga aaaaagggta ttatcgtatg ctttatattt tttaagagaa ggggatcaaa
     6301 tgaaaaaatt atggctttta ttaccgctac tgttgctttt atccgcttgt ggcactgcaa
     6361 aagaaacaag tagcaaacaa gaaataaagg atttgaaaaa agtgacactt gttttggatt
     6421 atgtaccgaa tactaatcat actggcatct acctagcaaa agaaaaaggc tattataaag
     6481 aggctggttt aaatgtccaa atcatcgaac caggagataa ttcgacaagt attggtctag
     6541 taggtgcgga caaagcacaa tttggtgtaa gttatcaaga agatgtcact tatgctcacg
     6601 cagatggaca gaatatacca gtcaaagcaa ttgctacagt gatcaaacat aatacttctg
     6661 gattcgccac cctatcagac agtaacatcc attcaccaaa agattttgaa ggaaaaacat
     6721 atgcaggttg gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg
     6781 gcggggactt ttcaaaactg acaatggtag gaagcaatgg agaagggcca gaaagcttag
     6841 gaaaatcctc agatatccaa tggtactttg aaggttggga tatgatcaaa gcaaaagaag
     6901 cgggtattga actaaactat atcccattga aagaattaga tgaacggtta gattactata
     6961 ctccggtaat tattacgaat gatcaactga tcaaaagtga tccagaattg gtacagtctt
     7021 ttatggatgc cacgaaaaaa ggctatcagg aagctattaa ggatccaaat gacagtgcta
     7081 aactgttgca aaaatatgca aaagaaaatg atcggacgtt tttagaagaa tcacaagcat
     7141 tcttgtcaaa aaattatacg gatgatcctg agaattgggg attgatggaa gaaaaagttt
     7201 ggcgcaatta tacagctttt atgcaggaaa acggattgat caagcaagat gtaccttcgc
     7261 aagaactatt cactaatcag tttataaaat aggagtgata gatatgtcaa tccaaatcag
     7321 ccacttgaat gtagtcattg aaaacctgaa aatattagaa gacatccatt tcactatccc
     7381 taaagatacc tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa
     7441 aactttaaca ggagtccagc ctatgacttc tggaacgatc aaagtagacg atcaaaaagt
     7501 aactggtctc catacagcat ttagttatat gccgcaagaa gatatgttac ttccttggct
     7561 gagcgtctat caaaacgtta cgttatatca aaaaatcaat catctaacaa tcaatgaaga
     7621 acaagtaagg gattatctgg atatctttgg gttaaacggc tatgaacatt ttttgccgga
     7681 acaattatct ggcgggatga agcagcgaac ggccttgtta cgaacaatca tgaatccttc
     7741 ttcttacttg cttcttgatg aaccatttgg agcgttagat gctatgacca gagggcagat
     7801 gcaagactgg atgttgaagc tgcctaaaaa agcaaagcgg actactttac ttgtgacgca
     7861 tgatatcgaa gaagcaattt atttatcaga caggattctt gtgctttccg cccgtccggc
     7921 acatgtgatt gctgagattc aagtgcctga aaaacgacgg tcacgtgaat ggctactgca
     7981 gcaatcagag ttgaagcaaa cgatttatca gttattggca ggtgaattag atgttaagtg
     8041 atgctcattg tcatttagat gggagtcaga gtttagcact ccttcagcag gaacacacga
     8101 ttttgacgat catcaattgt gattcgccag aagaatggaa ggaaaacaga cagcttgcag
     8161 caagtaagac tcaagcctta agttacggta tccatccttg gaaagcagat agctatacgt
     8221 ttgaacaagt tgagcctttt ttgaaaaaag caaggatcat cggtgaaata gggttagata
     8281 atatttggac gaatgtaccg atgactacac aaaaaaaagt atttgagcga cagcttgctt
     8341 ttgcagccat aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa aaagagatct
     8401 taaattatat ccaagcttac cctaatcact atttgatcca ttggtattct tctttggact
     8461 atcaacaaga ctatattgat ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga
     8521 atccagcagt ctggcaacta gctaaagcag tcccaatcga ccacttactt attgaaacgg
     8581 atggactaca tgcagcagag tgggctttag gggggatttt ttcagaaaaa gattatgtct
     8641 cactgcttga agaacagtta aagacaatcg ctgagatcaa agagctatca gttgaagaag
     8701 ttaaaaaaaa tacttatcaa aacttggaac gtttcattag attaggtaaa taaaaaagag
     8761 atgtgacaca actagtgtcg cacctctttt tggctttctt tgtataaaag taaactgttt
     8821 gattaataaa tgaatcttag taccaaccgt ttgcttccca gaatgctttc gccgcttccc
     8881 aagaaccata acgtgaagta acatattgtt ctgctactct ttcttggttt gcagcagagt
     8941 agtcaccatt taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt ccgttcgttg
     9001 ctgtgtaaga accgcttgat tctttttgtg cgatccattc ttttgcagag cttgtgcttg
     9061 ctggagtagc agcagtttct gttacagcag gagcaggttg tgtttcagca acaggcgctg
     9121 cttgtttttc cgtttgaacc gtttcttgta caacaggttg ttcagcttgg actggtgctt
     9181 cttgaacggc tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt gggattgtta
     9241 attgttgtcc tgagta
//
LOCUS       DASJSZ010000096         9163 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.100, whole genome shotgun sequence.
ACCESSION   DASJSZ010000096 DASJSZ010000000
VERSION     DASJSZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.100"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="U4B03_002397"
     CDS             6..305
                     /locus_tag="U4B03_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2069210.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="U4B03_002398"
     CDS             378..1739
                     /locus_tag="U4B03_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEN2069211.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="U4B03_002399"
     CDS             1761..2021
                     /locus_tag="U4B03_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069212.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="U4B03_002400"
     CDS             2035..2787
                     /locus_tag="U4B03_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEN2069213.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="U4B03_002401"
     CDS             complement(2881..3486)
                     /locus_tag="U4B03_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2069214.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="U4B03_002402"
     CDS             complement(3539..4513)
                     /locus_tag="U4B03_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEN2069215.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="U4B03_002403"
     CDS             complement(4891..5610)
                     /locus_tag="U4B03_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2069216.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4B03_002404"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4B03_002404"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN2069217.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="U4B03_002405"
     CDS             7161..8558
                     /locus_tag="U4B03_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2069218.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="U4B03_002406"
     CDS             8639..9079
                     /locus_tag="U4B03_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2069219.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
BASE COUNT         2894 a         1646 c         1897 g         2726 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact tta
//
LOCUS       DASJSZ010000097         9018 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.101, whole genome shotgun sequence.
ACCESSION   DASJSZ010000097 DASJSZ010000000
VERSION     DASJSZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.101"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1101
                     /locus_tag="U4B03_002407"
     CDS             <1..1101
                     /locus_tag="U4B03_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2069220.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1609)
                     /locus_tag="U4B03_002408"
     CDS             complement(1280..1609)
                     /locus_tag="U4B03_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2069221.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1599..1871)
                     /locus_tag="U4B03_002409"
     CDS             complement(1599..1871)
                     /locus_tag="U4B03_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN2069222.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="U4B03_002410"
     CDS             complement(2322..3125)
                     /locus_tag="U4B03_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2069223.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="U4B03_002411"
     CDS             complement(3103..4227)
                     /locus_tag="U4B03_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2069224.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="U4B03_002412"
     CDS             complement(4243..5058)
                     /locus_tag="U4B03_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2069225.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="U4B03_002413"
     CDS             complement(5048..5920)
                     /locus_tag="U4B03_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2069226.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="U4B03_002414"
     CDS             complement(6005..7294)
                     /locus_tag="U4B03_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2069227.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7522..8166)
                     /locus_tag="U4B03_002415"
                     /pseudo
     CDS             complement(7522..8166)
                     /locus_tag="U4B03_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(8643..>9018)
                     /locus_tag="U4B03_002416"
     CDS             complement(8643..>9018)
                     /locus_tag="U4B03_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2069228.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2778 a         1933 c         1427 g         2880 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacagccaga ctgtagacaa aagctgttcc
     8701 aaactcgtaa ctcgagcttg gaacagcttt ttatctactt cgtttcgaat ttttgaacaa
     8761 aaaaataaag gctccttgcc tttccatagc caggtggcta atttctttaa attcattgca
     8821 gcaaacacta gcatcgtatg cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc
     8881 atcccatgct tttcttttgc atccgcaaat attcgttcaa tcgtttgttt ccgttttttg
     8941 taaattgatt tattcagatc tgttaggcga agatgttcca cttcatccat cagatctccc
     9001 caaacatgtc tcgtgatg
//
LOCUS       DASJSZ010000098         8940 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.102, whole genome shotgun sequence.
ACCESSION   DASJSZ010000098 DASJSZ010000000
VERSION     DASJSZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.102"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(13..1152)
                     /locus_tag="U4B03_002417"
     CDS             complement(13..1152)
                     /locus_tag="U4B03_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEN2069229.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="U4B03_002418"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="U4B03_002418"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEN2069230.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /locus_tag="U4B03_002419"
     CDS             complement(2965..3720)
                     /locus_tag="U4B03_002419"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEN2069231.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /locus_tag="U4B03_002420"
     CDS             complement(3824..5014)
                     /locus_tag="U4B03_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEN2069232.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="U4B03_002421"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="U4B03_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN2069233.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="U4B03_002422"
     CDS             complement(6166..7257)
                     /locus_tag="U4B03_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEN2069234.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="U4B03_002423"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="U4B03_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN2069235.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="U4B03_002424"
     tRNA            8800..8871
                     /locus_tag="U4B03_002424"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
//
LOCUS       DASJSZ010000099         8857 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.103, whole genome shotgun sequence.
ACCESSION   DASJSZ010000099 DASJSZ010000000
VERSION     DASJSZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.103"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..470)
                     /locus_tag="U4B03_002425"
     CDS             complement(<1..470)
                     /locus_tag="U4B03_002425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069236.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNISVMRILSHIWFSIKTLLFIRKEKPEIIYCR
                     IPPNTLVFFIAKFSRCNRVIFDVYDLWPESMPGIGNKYKFFTDLWKKLRDQYLS"
     gene            complement(463..1077)
                     /locus_tag="U4B03_002426"
     CDS             complement(463..1077)
                     /locus_tag="U4B03_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_065828827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN2069237.1"
                     /translation="MYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGSPVIFK
                     QQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPELWNIL
                     KGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFEKDIEY
                     VENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(1085..2167)
                     /locus_tag="U4B03_002427"
     CDS             complement(1085..2167)
                     /locus_tag="U4B03_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037946024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN2069238.1"
                     /translation="MSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEEELAEYVGASHAA
                     ALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPVFVDSDYETWNMN
                     PEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIEDAAESLGTTYKGK
                     YTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSREKARHYQHNEIG
                     YNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQFMPENNWNEPNYW
                     LSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGTDVAQTLFEKGIC
                     LPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(2205..2837)
                     /locus_tag="U4B03_002428"
     CDS             complement(2205..2837)
                     /locus_tag="U4B03_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN2069239.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2848..4665)
                     /locus_tag="U4B03_002429"
     CDS             complement(2848..4665)
                     /locus_tag="U4B03_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2069240.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4678..5442)
                     /locus_tag="U4B03_002430"
     CDS             complement(4678..5442)
                     /locus_tag="U4B03_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEN2069241.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5470..6168)
                     /locus_tag="U4B03_002431"
     CDS             complement(5470..6168)
                     /locus_tag="U4B03_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEN2069242.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(6180..6959)
                     /locus_tag="U4B03_002432"
     CDS             complement(6180..6959)
                     /locus_tag="U4B03_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEN2069243.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6975..7919)
                     /locus_tag="U4B03_002433"
     CDS             complement(6975..7919)
                     /locus_tag="U4B03_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2069244.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7965..8744)
                     /locus_tag="U4B03_002434"
     CDS             complement(7965..8744)
                     /locus_tag="U4B03_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069245.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2411 a         1821 c         1491 g         3134 t
ORIGIN      
        1 ttactgagat attgatctcg tagctttttc cataaatcag taaaaaattt atatttgttc
       61 cctattcccg gcattgactc tggccataaa tcatacacat caaaaataac tcgattgcat
      121 ctactaaact tagcaataaa aaaaactaag gtattaggtg gtattctaca ataaataatt
      181 tcaggctttt cttttcgaat aaataataat gtttttattg aaaaccatat atgagataaa
      241 attcgcatta cggatatatt ttttttatat ccgatcgttt ttatcacatg acgattttta
      301 atcatatgcc tttctttact ggatagaaat tgttttctta tatgtgagta atcagataca
      361 acattaaaat attcgatact gttttccgtc agaatttcct ctaatatatt cgttcgactg
      421 atataatcaa aaggatgaat aagcagccac ttttttttat tctcactcat ctatcttcgc
      481 accctcaaat ttctcagtag tcccacttgc tactgatgtt attccatcac ggacaataac
      541 tttctttagc gttaaaaaaa atatttttat gtctttcatc attgaaatgt tctccacata
      601 ttcaatatct ttttcaaatt tttctttcca actaattgca ttacgaccac tgacttgagc
      661 taatccggtt aaacctgggc gcacaaaatg tctttttcgt tgttcattac tgtatagtga
      721 aagatactca actaacagag gacgcggtcc aataatactc atatctcctt ttaaaatatt
      781 ccataattct ggtagttcat caagactagt cgaacgcaat attttaccaa attgagtcaa
      841 tctgatatcg tctgataata aattaccttc ttcatctctt tgatctgtca ttgttcgaaa
      901 tttatacatc agaaaaatct tttcatgttt tccaggtcgt tgttgtttaa agatcactgg
      961 cgatccaagc tttattcgaa ctaggattgc aacaaacgtc atcagtggcc ataacattat
     1021 taaacctaaa attgaaaaaa gaatatctat caaacgttta aagaactttt cgtagatacc
     1081 tctattagac attttgcatc actcgcttaa ttgtattagt tactcgatac aaatcatcat
     1141 cggtcatttt agaatctgaa ggtaaacaga tacctttttc aaacaatgtc tgagcaacat
     1201 ctgtaccaat atactcatac ttatcgaaaa ctggctgtaa atgcattggt ttccatatcg
     1261 ggcgggcttc tatattattt ttatctaact catcaataag atgattcgta tttattccct
     1321 ttactgttat gacacttaac caataatttg gttcattcca attattttct ggcatgaatt
     1381 gtatatttga caatttagac aattcttttt tataaaaact atgaatatat cgttttttct
     1441 caattctttc atttaaaact ttcaattgtc ctcgaccgat acctgcaacg atattgctca
     1501 ttcgataatt atagccaatt tcattgtgtt gataatggcg tgccttctca cgagattgcg
     1561 tagcccaaaa cctcactttt tccacctttt ctttgtcatt agacacaagc attccgccac
     1621 ctgaagtcgt gattatctta tttccattaa aagaataaat tccatagtcg ccgaatgtac
     1681 cagtatattt tcctttatat gttgttccta atgattctgc agcatcttca attaaaacaa
     1741 cattatgttc tttgcatatt ttagtaattt catctagttc agctgataaa ccgtacaaat
     1801 gcactactaa tacagctttt gcgttaggat atttttctag cgctgttcgc aaatgttcag
     1861 ggttcatatt ccaagtttcg taatcactat caacaaatac tggaatagct ccttgataga
     1921 tgattggatt ggcagtagct gagaacgtca aagactgaca aatcactata tcatctttac
     1981 cgaccccagc cgcttttaaa gctagatgta tagctgctgt tccagaagat agtgcagctg
     2041 catgactagc accgacatat tctgctaatt cctcttcaaa accattgaca ttcataccca
     2101 aaggagccac ccaatttgta tcaaatgctt cttttacata ctcttgttca tatccttcat
     2161 cactcatatg aggagatgct aataatattc gtttattttc caacttaatc ccatctttct
     2221 atgactttgg taggtacgcc aacattttta gtcttattat taatattttt tattactaca
     2281 cttcccgcac ctataatgtt catatttcca attcttagat tttgtattat tttagtacct
     2341 actccaattt catttttatc tcctaataga acatgtcctg aaatatttac acttggaaaa
     2401 attgaattat aattaccgat ttgagtatca tgtccgatag tagagccgat attgatcata
     2461 ttgaaatttc ctaaaactac gtcagcagtg taagtcgtat agggcatcaa aatattgcca
     2521 attcctaact gaacatttat gcccactagt gcacttgatg caactaaatt agggaaaatc
     2581 aatttattat ttttatttat cttttgatat attttctctt tgatatctgg cgaagcgatt
     2641 cccagaaaaa tagcagtttc ttcttctaat tctaataacg agtctattgt tcccaataca
     2701 gtttttccca ctaattcttg attttgcatt tttaacgaat catcgacaaa accaatcaat
     2761 tcatatctag agaaactttc tagtagaaaa gcaatctctt tgccaaatcc accagctcct
     2821 acaataattg ctttcacttt actcccccta cttattcgaa gcattcgcaa aagtgacaat
     2881 gtctttagcc aattgttcat cgtcttgcgg caggctcttg acaaattcca tcactgtttg
     2941 catggaataa cctttgatgt ttcccacaaa gatcttctcg taaactgctt catcgtttct
     3001 ttccttatct aacaatagtt cttcatatag tttttcccct ggacggatac ctgtttcaat
     3061 gatttcgatc tcgtcttcgc tatatccgct caatcggatc atgttttttg ctagatctac
     3121 gattttcact ggttcactca tatctaaaat aaagatttcg ccgccttttg ctaacgcccc
     3181 agattgaata actaaacgac ttgcttcagg aatcgtcatg aaatatcggg tcatacgaaa
     3241 gtctgttaca gtgactggcc ctccttgagc aatttgttcc cggaatactg gaatcacact
     3301 accgcgagaa ccaaggacat tcccaaatct gacagctgag aatttcgtac atccttcttc
     3361 attcaaccca gtcacgatca tttcagcgat tcgttttgtt gaacccatga cgtttggcgg
     3421 attgttggct ttatctgtcg agaccatgac gaagtttttt actttcgctg ctttggctgc
     3481 ttctgctaca ttttttgtac cgaaaatatt gtttttgact gcttcttttg ggttatattc
     3541 catcaatggt acatgcttat gagcggctgc atggtagacg atatctgggt ggtattgttc
     3601 catgatctca ttgattttct tgcgatcttg gatatccgca atgatcggca cgatttctgt
     3661 aggacacttt tgatgatgcg tgcgtaattc acggtcaatc aagtaaatcg agttctcccc
     3721 atgacctaaa agtaatagtt ttgcagggtc aaattggatg atttgccggc agatttctga
     3781 accgattgat cctcctgcac cggtgacaag aatgactttg tttgtgatct ggtctttgat
     3841 ggattcgata tccaactcta cctcatctct tcccagtaat tccactacgt cgatggtttt
     3901 taattttgat acgctgattt tccctgaggc aagttcttcc atagaaggca tcgtattgac
     3961 tttcacatga gcagattcga ctaactcgaa gattcttcgt aatttttttc ttgataaaga
     4021 agggatagcg atcgttacca tttggatgtc atatttttcg atcaattccg gtatatcttt
     4081 taatgacccc agtacttttt ttcctgaaag ataggtattt cttttattcg gatcatcatc
     4141 cacgaatcca atgacatgga tatcttgagc cgttttagaa ccaagaaagc tgttgtataa
     4201 aatacgtcca ccttcgccgg ccccaacgat caaggtattt ttagcggaat ctgttgatag
     4261 atacctcata ttttttgttt cgatatagat acgccagatc aatcggctgc caatgatcag
     4321 taatagcgaa agtaggtatg caaagatcac taaacgtaaa ctgtattgtt tatttatgaa
     4381 aagcaatatg atgatacttg aggccgcact tgcagataac gaaccaaaaa tcgcaatgat
     4441 ctctcttaaa tttgtataac gattgattcg agtaaaaact ttgaataaac taccatagaa
     4501 taaataaaaa ccaatcgaca aaccgataga gattaaaata aggtccattg gtatagctac
     4561 aaacggcttc atgaatttag ctgctgcaat attcgcagca atcaacaaca agctgtccac
     4621 aaataccaat gtggcaattt ttcttcttct tgttaataca aacaccctac attcccccta
     4681 aaataaacca aacttcttct tttttacctc ttcatagact ggataactga tctcatcccc
     4741 gttgatcagg tcctttgcga cttgtttcat ctgtgctact ttttccttac caaagtcctt
     4801 ggcgatttgc tcataacatt cttttaagta aaaggtacgt ttagagaccc catgggcatc
     4861 ggaagcagcc atttgcacga ggttatgttt gaccatcatt ttggctgttt tctggatgtc
     4921 ttttccaaat acaccgacat aacttggtgc ggtgagttga gccaagcatc ccatatctag
     4981 gaatggcagc aaatgatttg ggtcttctcg aaaatgcgcg ttgcgttctg gatggacgat
     5041 cactggtgtc tttcctagtt gtaacaactc aaaaaagagc cattctgcat ataacggcac
     5101 ttccagtgtc ggaaattcga ttagaagata tgtatcatct aaatctgtaa ataaaatttc
     5161 atctcggtgg atgtcttcaa tcagtgttcc ggtaatacgt acttcttgtc cttccaataa
     5221 cgtcaaaggc aattgtcttt tctctaattc tgcttgtaac gaagcgacta gagaaatgac
     5281 ttgtgatttt tcattgctgt attttccatt attgtgatgg ggtgtgcata aaatatgtgt
     5341 gatcccttgt tgaatcgctt tttcagccat ggcgatactg gcatctatgt tttctgctcc
     5401 gtcatcgatt cctggcagga tatggcaatg tagatcgatc atttctcttg tctcctctct
     5461 ttttatttct taattcccgt agtaatagcc agcatctctg ctgtgttctg ctccattgta
     5521 tacaacacca aggactcgcg cttgtaccat atctaacagt tcttttgctt tagtcaatga
     5581 ttcttttcgt gacgtatttt cccgtacgac caataaggtg ccatccgttt ttgaagacat
     5641 gatctgtgca tctgttacgg caacgactgg tggcatgtcg aaaatcacta catcaaatag
     5701 ttgtcttgct tctgctaaaa tctgatccat tcttggcgaa cttagtagtt ctgatggatt
     5761 tggtggttta ggtccacttg gcagaatcga caaattgtcg actggtgttc gttggatctc
     5821 atctgctaca cttcctgatg aactaagaac agtacttaat ccgcttgcgt tgttcaactt
     5881 gaacgtttta tagatgacgg gcttacgtaa gtctgcatcg actagcagta cccgttgacc
     5941 agatttggca aaaacaacgg caatgtttgc tgcagtggtg gattttcctt ctccaggacc
     6001 tgatgaagtc acgacaatcg ttttgatttg ctgaccagct gcggaggcaa actggatatt
     6061 cgtccggatc gtacgatatt gttcggcgat tggtgaagat gggtttgtta atgtgatcaa
     6121 actgactgca tgagtttgag ttgctttttg tttctgtgtt cgtgccattt ttcttcctcc
     6181 tagactcttg agcggcttct gcgtgatggg gctgttttat cgtttgtagc ttttggtcgt
     6241 gcaggagctg atcctttttt acggatacca gcgttcaatt cttttgctga catttctgga
     6301 actgttccta agattgggaa tcctaatgta tccgtcacat atttttcatc tttcactgtc
     6361 cgatcaagta attcgaataa gaatgccaga cctacgccta ccatcattcc taacactaag
     6421 ccaatcacta gattcaattt attatttggc gaaacaggtg tcgtattggc aactgcacct
     6481 gaaataatcg agattttatc gacattcatc acgtctttgg cgttttcttg gaaaacttgt
     6541 gaagttgtat tagcgatctg ttgagcagtc actgcattcg tgcttattgc ctggatcgag
     6601 aacatttgtg aattttgtga ctgattgacg gagatcgcat ctttgatttg tccagcagtc
     6661 attttcacat tgtcttctgt ttctaaacga tctttgactt catttagtac taaatcactg
     6721 acgatcatat ctttatacgt attgatcatt tgcaggttcg tattgacatc attgacattt
     6781 gctgtgtctg attgtggcag tgtcacgatc aattgtgctt gtgaactgta ttttggtgtc
     6841 aagatgaaaa atgttacgat tcctgagatg gccaagccag caaacattga gatgatgatt
     6901 gttaccaagt gtttgcgtaa tgtttcaaaa atttctttca agctgattgt ctcttccatt
     6961 ttttctccac tttcctattt attctctaga ttcatttgat cttttaattc ttttttcaca
     7021 cgatcaagtt cttgatcgct gactttttga taggagatat tatcttgcat gaagccttct
     7081 ccttgcagct gatcttgctt gatcgtacca aatgcctccc gataatctaa agcgattttt
     7141 ttcatttcat caaagctcat gtctgttttc atattcgact ctagagcatt caaaacggat
     7201 tgatagttgc tgacaccgtt taaactcaat actttttttg ctacaccttc tacgatcttt
     7261 ctttgacgct cttgtctacc atagtcacca tttggatctt cataacgcat acgagaataa
     7321 gataatgcct gatccccgtt caatgtaatt ttaccgattg gaaagtcata gccatcttgt
     7381 gagaaagtga ggtcattatt gacttcgata ccaccaactg catcaacgag ttcctttagc
     7441 cctttcatat tgatagagac atagtgatcg attgggatgt ctacatattt ttgtagggta
     7501 tccatcgcca tggcagctcc gccgaatgcg taagcgtggt tgatcttgtc ttttgtgtca
     7561 cgacctacga tgtcgacata cgtatctcga ggaatgctga cgatcgttgt ctgtttgtct
     7621 tcaggactga ctgtggcaac catcatcgta tctgatcttc cttgatcgac ccgacctaaa
     7681 tctccagtat caatgcccat caatagaaca gagaaagatt cttgtttgtc cagatcaact
     7741 ggtttcgttc gttctccttg ggttcgttcc actgattcgt aagttttctc aatagaatgt
     7801 gagagatcca gatataattt tgctcctact cctacaattg ctaatactaa tacgataaga
     7861 actgctaaaa taatcagaaa tatttttttc cctcttgatt ttcttcttcg tcttgccata
     7921 gtttcctccg tttgctaacc ttgcttgaat caacgtttta actgtcaata acttatattg
     7981 ctatagccca tttgctgagc aacagtttgt gcttttgttg cgtatgctgc gcttattttt
     8041 tcattccgga tctgctctat ttgactgagc gccgtcaaat attgatcgta agtagcggtg
     8101 gcagtcactt gaccatcctt caacatcgta tcgaataact gatctagttg cgtgacttgc
     8161 gcgatctgag tatcagcgaa tcctagatat tgcataacaa ccgttttcca aggactatct
     8221 ttaaaaaagc tcatattttc acgtacacga gcgacatctg cacttgtcac cttttctttg
     8281 atgacgacat cgtctacagc ttgctgccag tttgatacat ttttagtaaa taaatcgttg
     8341 acagctgtct ggatcttcag ttttgattct gcactatcta actgtgtcac gattgctttc
     8401 ttctcatctt ggattgcaac agcttcttta ggcaattctg attctttgat gtcgtaatca
     8461 tctgctgaaa gtttgacaga ggatagctta tttgacaaac ttgtgattgt ttcttctgtt
     8521 atttcagggt ttagaaaatt tttttctttt gtactataca gcttgtctac ttcttttgat
     8581 aaagaagtca aggtgtcttt ttgattggtg atggcagatt ctgcataatt gatccgttgc
     8641 tgatgttgcc gctgattatt gatatggtaa ccaaacaaac agatcgcaat agcacctgca
     8701 cctaccgcca cagcagtcaa tgacttgacg aatggctttt ccatagattc actcctaagt
     8761 attgatgata gataaattaa gatataattc acatacataa aaatgacagc tttttatttt
     8821 aaaaaacata attaaattaa tatattcaca gaaatat
//
LOCUS       DASJSZ010000100         8819 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.104, whole genome shotgun sequence.
ACCESSION   DASJSZ010000100 DASJSZ010000000
VERSION     DASJSZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.104"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            161..1184
                     /locus_tag="U4B03_002435"
                     /pseudo
     CDS             161..1184
                     /locus_tag="U4B03_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1527..1783
                     /locus_tag="U4B03_002436"
                     /pseudo
     CDS             1527..1783
                     /locus_tag="U4B03_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2170..2397
                     /locus_tag="U4B03_002437"
     CDS             2170..2397
                     /locus_tag="U4B03_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN2069246.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2413..3579
                     /locus_tag="U4B03_002438"
     CDS             2413..3579
                     /locus_tag="U4B03_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEN2069247.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3814..4857
                     /gene="hrcA"
                     /locus_tag="U4B03_002439"
     CDS             3814..4857
                     /gene="hrcA"
                     /locus_tag="U4B03_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEN2069248.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4951..5514
                     /gene="grpE"
                     /locus_tag="U4B03_002440"
     CDS             4951..5514
                     /gene="grpE"
                     /locus_tag="U4B03_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEN2069249.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5565..7394
                     /gene="dnaK"
                     /locus_tag="U4B03_002441"
     CDS             5565..7394
                     /gene="dnaK"
                     /locus_tag="U4B03_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEN2069250.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7545..8711
                     /gene="dnaJ"
                     /locus_tag="U4B03_002442"
     CDS             7545..8711
                     /gene="dnaJ"
                     /locus_tag="U4B03_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN2069251.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3097 a         1426 c         1892 g         2404 t
ORIGIN      
        1 ataaagaata tgctaaggta agaatagtaa aaattcacat agatgaggta taataatgat
       61 atttttatat attacagata acttgacaca aaatgagttt ttttcgattt ttcaagttat
      121 ctaatgcaat tatattgata tcattaggag ctgcattgtt atgaatccaa cattgattac
      181 taagaaagaa ttactaaaaa aattagatat ttctactggt gtattagcta atttaattag
      241 aaatggcatg ccaaaagagg gagaaatgtt taatcttgat aagattatta catggcgaga
      301 aaattggtct aaaaacatat taggtgagtt agaagttggt agagtatata ctaacaaaga
      361 aatttcagag aagtttaaat gttccaaaca gggtggaatg agaagatctc atcaaactaa
      421 tactcttgta ctgtttagtg atcaaacagg aagtaatgtt tataaagata agtggttaaa
      481 tggtatttta caatataccg gtatggggct aaaaggggat caagtattgg ataagaatca
      541 aaataaagtt ttagccaatt caaaaagtaa ttttgtaaag attcatttat ttgaaacatt
      601 taaaccaaaa gaacacacat atctgggaga agtatattta gcaggacaaa tatatactgt
      661 taatgaaaaa gatagttcgg gcaacagcag aaaagtatat aaatttccat tagctttaat
      721 aaatcaagag caattgattg aagataaaga tatataacca ggaagaaaat caaactagac
      781 atattcgtaa tttatctaat gctaaattag aggaagaagc aagaaaagtt agtaattata
      841 ataaaaaaat ggctgcaaaa tatgttaacg aagaggagtt ttgcttctca aaaacaaaaa
      901 tatataaacg taatcccttc gtatctgaat atgttaaacg acttgcaaaa ggtatttgtc
      961 aactttgcca agaaaaaggg ccatttataa aagacggtgt accttattta cattgtcatc
     1021 atatagaata tttgtcgcaa ggtggaaaag atgtgattga aaactgtatc gcgttatgtc
     1081 ccaattgtca tgctagaatt catgaacttg agttacctga cgataaagaa aaattgctga
     1141 agatcgttga aaaaagagaa aatagctttt ctaaaatacc gtaatttatt tttataggac
     1201 aaataagcta atgtgagcta tgccttctat ataagactaa gttctattat aagtaacgaa
     1261 gtaattctcg ttccttcctg tagtaatatt ttttaaataa cctataggct tcttcaaaag
     1321 tacatcctcc agtaaattct aagtactgga ggattctttt tctagtgttg ctttactcat
     1381 gaaaaagtag ttggctactt catcaaaatt tctgaacttt tttaatgtat aataaagagc
     1441 tcgatcattt actacaagga tgctagctcc tacattttca cagtttttta gcaagtaaat
     1501 ttttctaaaa tcacatcagg aaatgcatga gattctttaa gagaggcaat tatagggcta
     1561 tgattacggt tcgtgtaagt gacgccaaaa aagaatggct ttcttatatg gctgaatttt
     1621 atgggattac tttatctgaa cttctaaaaa actattcgat ggaacaacta gaagatgaat
     1681 acaatcgtca aacggctgaa attgcccata aacattggat aagaagtggg aaaacaacag
     1741 tattcatgga agaatcctta atgaatttgg tgggctggaa tgacctgccg tttaaaattc
     1801 acgcccgaaa ttcagaagca gctctgattg gcaaatacgt gtattggtag agatagaaga
     1861 taatcaattc gttgttatcg ctattcaagt agggcaccga agaaatgtct atgattaaga
     1921 gactgtttat ttttacaaaa aaataataat tggaatctag ttaaaactct tgaagtgaga
     1981 gatttgacta gattttttta tgaataaaat aaggacaaaa ttgtttagga agaatcatga
     2041 gtgtaaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
     2101 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
     2161 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
     2221 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
     2281 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
     2341 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
     2401 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
     2461 catatcccat tttgcgagca tatctgctat tactgtgact ttaataaagt ttttttagaa
     2521 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgagatt aatgctggaa
     2581 agtcatccta cagcgaattt ggaaacactt tatgtaggag gcggaacacc aacttcttta
     2641 tctgctaaac aacttgatcg actattatca ggtgctagag agatcctgcc ttttcaaaat
     2701 ggtaatgagt ttactgtaga agcgaatcct ggtgatttga cccgtgaaaa acttcaagtg
     2761 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga caatcgctta
     2821 ttgaaaaaaa ttggacgtaa acatactgca gaagatgtgt atcaaacaat gcaattttta
     2881 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
     2941 ctagaaggat atcgggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
     3001 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
     3061 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
     3121 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
     3181 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
     3241 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
     3301 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
     3361 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
     3421 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
     3481 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacttttttg
     3541 gggaacaatg tgtttgagcg ctttttattt gaaaaatagt ttgaagaata tttgtttttt
     3601 atcgaaactt gaaaagaaaa agtttagaaa gtttgacaat gaggtattga agtattgata
     3661 taactggatt tacatcaatt atttagaatt agcactcttg tgtttagagt gctaaaaaat
     3721 ggagtctttc tcttgacagg agaagggctt cttgctatat ttataagtga attagcactt
     3781 agaatatatg agtgctaatg agaggtgagg aagatgttaa caaagcgaca aaacgacatt
     3841 ttacgtctga tcatccaaca ctatacgagt agtggcgtgc ccgttggttc caaaacatta
     3901 atgtctgagg gagtcaaggc cagctcagca acgattcgca atgatatgaa agcattagaa
     3961 gatgaaggat tgctgttgaa gactcactct tcttctgggc ggattccatc tgctttaggc
     4021 tatcgttatt acgtggatca tttgttaaaa cctgctcgcg tagcagaaga tgatttgctg
     4081 ctgattcgtc agtctctagg cagagaattt catgaaatca atgagattat taagcaatct
     4141 gctgagatat tatctgagct gacaagctat acgacttttt cattgggacc tgaaataaaa
     4201 gaccgccgac tgactggctt tcgtatcgta cccttgaata atcgacagat tattgctatc
     4261 gtcgtaacag atatggggaa tgtagaaagc caagtgttca ctcttcccga atatatggga
     4321 agtcaggaac tagaaaaaat ggtacagatt gtaaacgacc gtctagtcgg agatcctctg
     4381 atgacggtgt accataaact tcgtacggag attccgatga ttcttcataa atatttccag
     4441 acgacagaag ggatgtctca gttatttgac accgttcttg gtcatgtttt tgaagaaaag
     4501 gtttatgtca gtggacaaat gaatctcttg gattatgagc cccgtcaaga tcttgatcag
     4561 ttcaaatcga tgttttcttt tatgaaagac tcagacggac ttacacaaat gatcactccg
     4621 atggatagca atattcatat caagattggt gctgaattag gcaatgattt attgcagaat
     4681 atgagtatga ttcaagcaag ttacgagata gatggtcatg gaagaggtac aatagccttg
     4741 ttagggccta ctagtatgcc ttattctcgg atgttcggtc tagtcgatgt ttatcgaaga
     4801 gaactagcag ctaaacttgc tgattactac cgctcgcttg acttgtcaaa ctcgtaatgt
     4861 gaataacgaa gctatttgca gctgatcgtt ctctctttct tagtaagagc aatcaaacag
     4921 ctgactgaat aatgaaagga agactgtatc gtgaaagaaa atcaagaaga actagataaa
     4981 gaaatgacag atgctcagcc agaaccggaa atcgatgtcg aagctgcaga agattcgggt
     5041 atttcagaag ctgaagcgga agaattcgaa acagcgaaat taaaagctga gttagaagaa
     5101 atggaagata aatacttgcg ggctcgtgct gaaatcgcaa atatggcaaa tcgaggaaaa
     5161 aatgagcgtg agcaattaca aaaataccgt tcacaagatt tagccaaaaa attacttcca
     5221 tcaattgaca acctggaacg tgcattagcc acagaagtgt cagatgatca aggggcaggt
     5281 ttgaaaaaag gcgtggaaat ggtcttggaa agtttgcgta acgcactgga agaagaagga
     5341 attgaaaaaa tccctgctaa aggagaagcc tttgatccta atcttcatca agcagtacaa
     5401 actgttccag cgactgaaga tacaccagcc gatacgattg tagaagtact acaagaaggc
     5461 tacaaattac atgatcgcgt gttaagacca actatggtca ttgttgcaca ataattgtgc
     5521 ggatataaaa cttacacttt caattaaaga aggagacgtt aattatgagt aaaattatcg
     5581 gtattgactt aggtacgact aactctgcag tagcagtatt ggaaggaaac gaagcaaaaa
     5641 tcatcgctaa cccagaagga aaccgtacaa caccttctgt tgtatcattc aaaaatggag
     5701 aaatccaagt aggggaagtt gcaaaacgtc aagctgtgac aaacccaaat acgatttcat
     5761 caatcaaacg ccatatcggt gaagcaggtt acaaagtaga tgtagaaggt aaatcatata
     5821 caccacaaga aatctcagca atgatccttc aatatatcaa aggattcgca gaagactatt
     5881 taggtgaaaa agtcgaaaaa gcagttatca ctgtgccagc ttactttaat gatgcacaac
     5941 gtcaagcaac aaaagatgct ggtaagatcg ctggtctaga agtagaacgt atcgtcaatg
     6001 aaccaactgc agctgctctt gcctatggtt tagataaaac agatcgcgac gaaaaaatct
     6061 tagtattcga ccttggtggc ggtacttttg acgtatctat ccttgaacta ggagacggtg
     6121 tctttgatgt attgtcaaca gctggcgaca accatctagg tggggacgac tttgataaca
     6181 aaatcatcga ctacatggta gcagaattca aaaaagaaaa cagcattgac ttgtctcaag
     6241 acaaaatggc tttacaacgc ttgaaagacg ctgctgaaaa agcgaaaaaa gatttatctg
     6301 gcgtatcaag cacacaaatc agcttgccat ttatcacagc aggagaatct ggaccattgc
     6361 atttggaaat gacattgaca cgtgcaaaat ttgatgaatt aacagctgac ttagtagaac
     6421 gtacaaaagt accagtacgt caagcgttaa aagatgcagg tctgtcacaa tcagaaatcg
     6481 acgaagttat tttagtcggt ggatctactc gtatccctgc agttgtagaa gcagtacgta
     6541 aagaaacagg aaaagaacca aacaaatcag ttaaccctga tgaagtagtt gctatgggtg
     6601 ctgctatcca aggtggcgtg atcactggtg atgtgaaaga tgttgtttta cttgacgtta
     6661 caccattgtc attaggtatc gaaacaatgg gtggagtgtt cactaaattg attgaccgca
     6721 atacaacgat tccaacaagt aaatcacaag tattctcaac tgcggcagat aatcaaccag
     6781 cagtagacat ccatgtatta caaggtgaac gcccaatggc tgctgacaac aaaactctag
     6841 gaagattcca attgacagat attccagcag ctcctcgtgg tattccacaa atcgaagtaa
     6901 cattcgatat tgataaaaac gggattgtaa atgtatctgc gaaagatcta ggtacacaaa
     6961 aagaacaaaa aatcacgatc aaatcttctt caggtctaac agacgaagaa atcgaacgta
     7021 tggtgaaaga tgcagaagca aacgctgaag cagataaagc tcgtaaagag gaagttgacc
     7081 tacgtaatga tgtagacgca ttgctattct ctgtggacaa aacattgaaa gaattagaag
     7141 gcaaagtgga cgaagaagaa gtgaaaaaag cagaaacagc tcgtgacgaa ttaaaagccg
     7201 ctgttgaagc aaacaacatc gaggaaatga aaactaaacg cgatgcattg aacgaaatcg
     7261 ttcaaaactt gactgttaaa ctatacgaac aagctgcaca acaacaagct caagaaaatc
     7321 cagaagcagc tcaaggcgga gcagatgatg tcgttgatgc tgattttgaa gaagtcgatg
     7381 gcgacgataa ataatcagtt cactgaattt cgagagaagg ccaaagttgc tactttggct
     7441 ttttcttttg aaaaatagtt tgaagtatcg gtttttgttt caaacaaaat atgatatgat
     7501 aacaagcgac gtgaaaatga tgagaaccaa taggaggaaa gcctatggca acaaaacgtg
     7561 attattatga agtcttaggg ctctcaaaag gagcatcaga agatgaaata aagaaagctt
     7621 atcgtaagct ttcgaaaaaa taccatcctg acatcaataa agaacctgat gcggaagaaa
     7681 aattcaaaga agtatcagaa gcatatgaga ttttaagtga cccgcaaaaa cgcgctgcat
     7741 acgaccaata cgggcatgca ggcacagatc caaattacgg tgctggtgga gctggtggcg
     7801 gatttggtgg attcggcggc ttctctggtg gcggcttcgg tggttttgaa gatatcttcg
     7861 actctttctt tggaggaggc ggtcggtcag tcgatccaaa tgcgcctcgt caaggtgctg
     7921 atttgcaata tacggtcgac ttgtcttttg aagaagcgat ttttggtgta gaaaaagaaa
     7981 tcaagtataa tcgggaagaa atctgtcata cttgtggtgg aaacggtgca aaaccaggca
     8041 cacaaccaac tacttgtcat aaatgtcatg gtgcaggtac gatcaatgta gaacgccaaa
     8101 cgccacttgg acgagtgatg agccgtcaaa cttgtgatgt ttgtcatggc acagggaaag
     8161 aaatcaaaga accttgtcca acctgtcatg gctcaggaca tgagaagaaa gcacattcag
     8221 ttaaagtgaa tgtaccagct ggtgtagaag aaggacaaca aatgcgttta gctggtcaag
     8281 gagaagctgg agaaaacggc ggaccatttg gtgatttgta tgtggttttt cgtgtagaag
     8341 aaagtgatat ctttgatcgt gaaggttctg aaatttacta tgaattgcct ttaagttttg
     8401 tacaagcagc tttgggggat gaagtccaag taccaactgt ccatggagat gtgaaattga
     8461 agatccctgc tggaacacag acaggtacga acttccgttt acgtggaaaa ggcgctccga
     8521 agttacgtgg tggtgcaaca ggcgaccaac atgtcaaagt aaaactgatc acaccgaaaa
     8581 atctaaatga acaacaaaga gaagcattac gtgcattcgc tgaagctggc ggcatcaaag
     8641 ttgaagaaca gcaagaagac ggtttcttcg ataagtttaa agatgctttt ggcgggaaaa
     8701 agcggaagta gtttttctag taaaaatata aagtagactt gcccaaattt tctatttcta
     8761 gtaaaaacgc taagtcatta ataattaaaa accagacgaa caatcatacg ttcgcctgg
//
LOCUS       DASJSZ010000101         8682 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.105, whole genome shotgun sequence.
ACCESSION   DASJSZ010000101 DASJSZ010000000
VERSION     DASJSZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.105"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(280..2661)
                     /locus_tag="U4B03_002443"
     CDS             complement(280..2661)
                     /locus_tag="U4B03_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN2069252.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2651..3298)
                     /gene="recU"
                     /locus_tag="U4B03_002444"
     CDS             complement(2651..3298)
                     /gene="recU"
                     /locus_tag="U4B03_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEN2069253.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3362..3901
                     /locus_tag="U4B03_002445"
     CDS             3362..3901
                     /locus_tag="U4B03_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEN2069254.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3991..4410
                     /gene="gpsB"
                     /locus_tag="U4B03_002446"
     CDS             3991..4410
                     /gene="gpsB"
                     /locus_tag="U4B03_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEN2069255.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4432..4799
                     /gene="rnpB"
                     /locus_tag="U4B03_002447"
     ncRNA           4432..4799
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U4B03_002447"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5008..6174
                     /locus_tag="U4B03_002448"
     CDS             5008..6174
                     /locus_tag="U4B03_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEN2069256.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6220..7716
                     /locus_tag="U4B03_002449"
     CDS             6220..7716
                     /locus_tag="U4B03_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEN2069257.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7797..8480
                     /locus_tag="U4B03_002450"
     CDS             7797..8480
                     /locus_tag="U4B03_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2069258.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2733 a         1572 c         1844 g         2533 t
ORIGIN      
        1 tgataataac aatgggatta ttaagaagtg gcactaaata aaagactagt gtgtatttag
       61 ccgatttaat tataaacaag taaatggata aagtatatgt aatgataaaa gaaaaatgga
      121 ttttctaatt tttaagctaa atgttagatc acacatgtat atgctggata gtcttataaa
      181 aaatcatgta atacagcttg gtacatgatt tttatttgta gctgtttttt acatagacac
      241 aaaaaagaaa ccacgcttgt agacgtggtt tcttttttac tatctgtttc cagaagctga
      301 ggtgctgctg gatggtgtat ggtcatttgc agcatccgct ccgctagttg ctggctcact
      361 ggaagaagca ggaggagcag atgaagacgg tgtcgagctt tcttcggatg aagaagaagg
      421 aaccgttgtt gaagacgctg gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc
      481 agcagttgat tcgctttgac tagtagagct agagctttga gtcgttgtct ctgttgttga
      541 gcttccagga gtctggacat agctggaaga tggaatagtt gttgatggtg taattgcatt
      601 cgatctagct gtatattgat ctttgtagta cagttcaccg cccacacgaa ttaatccgct
      661 tggcatctcc cagtctgtat ttgtcacgtt agctgaaaca tactgcataa gctcgcgata
      721 aacatcagag gctacgtgtg acgattctga agtgacaggc gtcatttttt tattgtaacc
      781 tgtccacacg gaaatcgaat aatttggcgt atagcccgca aataggatat ctggatacac
      841 cccagaagaa ataccaagtt ttgcatactc gtcatctgtg taattggatg ttccggtctt
      901 accggcttga tataacccag cgatttgcgc attcgtacca gttccttcag tgatcgtatc
      961 ttttaaaatg tctgtgatca tataagctgt ttctggagac atggctgttt ttccatctgg
     1021 ctcataagtt tcttctgttc cgtcttggaa gacgatttta ttaacatatt gaggtttgta
     1081 ataagtccct ccattggcga aagccgcata ggctgctgcc atttttaagg aagaagctcc
     1141 gtattttgtc ccatcttgtt cttccgtatt actagagatc gcattcgatt gatggatcgt
     1201 actgtattcg atcccaagat tttttaagaa agaagccact ttatctgaac cgacttcatt
     1261 gaataatttg actgctggaa cattacggga taagtaaagt gcttggcgta aagtaatggt
     1321 gcccatatat tgattatccc agtttcctac aggagtcgaa gtcccttcat agttatacgg
     1381 tgcgtcagta atagtttttc cagtagaata tttcaaatat tcaaaagctg gtccgtaatc
     1441 tgtgacaggt ttcatcgtag aaccaaaatc acgggaagta ttcactgcta agttgttgcc
     1501 taaagttacg tcttctgcga tgtgacgtcc gccaatctgg gcttttactt tcccggtatt
     1561 ggtgtcgata agggtggagg caacttgcat ctcgtcgtct gggtaagaga catactgatc
     1621 cgtgttgacg atatcatata gtttcttttg ggcgtccaga tccaaattcg tataaatctc
     1681 taaaccgtct gtgtagacat tcttatctgt tttttcttgt acttcattga tgacttcttt
     1741 gacataatta tcgacgatct tagtattgtc atcggattga gttagttctt gcagaccatc
     1801 cgttaccggt acattaacag cttgatcata ttctgtttga gagatttttt cgttttgaag
     1861 catcgtatac aaaacagtat ctcgtctttt cttcgcctga tctggataaa cataaggatc
     1921 ataggcggaa ggggcttgag gcatcccagc aagcaatgct gtttgcggta aagaaagttc
     1981 tgataatttc ttaccaaagt acatctcaga agctgtctcc atcccgtaca agccgtttga
     2041 catatacact ttatttacgt agtaggtcaa gatctcttgt ttcgattttt tttgttccaa
     2101 tcttacagcc atccacgctt cttgggcctt gcgtttcaga gtctgatcct cggcactggt
     2161 ggagaagaag gaaagtttga tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc
     2221 agaggtaaag ttagagaggg cagaaccgat gatccgaata gggtcaaccc cgatgtgttt
     2281 ataaaaacgg cgatcttcta ccgacacgat cgcatcttct aatgttttcg gtaattcatt
     2341 cgcagaaatt ttttcacgct tttctgcgcc taaatcttcg aatagttcgc catcttgtgt
     2401 atatagtttg gaagatacgg tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt
     2461 tgcgtaatac cagaaaagtc ctacacctgc taaaaagagg atacagaaaa atgagagtat
     2521 tcctaaaagg atcttgataa ataatccttt tttatgtgtt ccagaggact ttccagaacc
     2581 tttgccagaa ttcttcttca cactcttttt aggagtaggc tgtttctgtc gtctagagct
     2641 tctagtttgt tcatttgcca ttcttgcttt ctccttcaat agataatgtt gataaataga
     2701 agtcaaccgc gtccaaataa ggaatccgcg gagaaatacc agttttgatc tctattccta
     2761 gttcttcgat caaagataaa ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt
     2821 aagcaatcaa atgaacacta ttcaaataaa aacagcgctt caaagacgaa aaccataaca
     2881 agacaaagca gaccccattt tgttcgaggc atgctttcat atgttcgatt tgatgggcat
     2941 ggaagttttt aaagggaaaa gatgttttgt tcttggtttc ttttgcttca aagtccaagt
     3001 aaaagcctcg atagacgcca ttatagtctg tagttgaggc ttgggtaaag taggcttcct
     3061 tgatcacagc agcacttctc ttaggatagt ctactttcac gatttggata ggggtaggct
     3121 ttttatggat aaccgccaac ccgcgggaaa gataatattt attactttca ttgattgctt
     3181 cttcaaagcg catcccccga ttgccgaatt cagtgatctg atttcttttt tcttgaattt
     3241 tttcgacggg agaggcattc ttataataag gttggccatt tggatagcgc aataccatct
     3301 ttaatcacac tcctaggcca cattatagca aaaaagcgga tagaaagaaa aggaatctga
     3361 gatggataag tcaaaagtaa tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt
     3421 taaagaaaat gatcctaaaa tccaagttgt taaaaatgtt ttaaaaaaag aactccatca
     3481 gttagcagaa gaagggctgg aatgggtact tgtcagtggg aatttgggcg tcgaactatg
     3541 ggctgtgcaa atagtagcgg aattgaaaga agaatatccg gaactatctc ttggtatttt
     3601 gtatcctttt tctgattttg gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt
     3661 agcagaacaa ttagcagatt atgtcgaagc tgttagccat caaccctacc aatcacctag
     3721 tcagctaaag aatcacactc gttttatact tgagcataca gtgggctgtc tgctgatcta
     3781 tgatgaagaa ttccctggaa aaacgaagta tttttttgaa gatgcacaga attaccaagc
     3841 agatcatccc tacgaaattc ggctgatcag tatggacgat cttcaaaatt ttagtgaata
     3901 atcgatattg tgtttgattt atcaactttt ttcctataca atgaaaaagg tagaaatgtt
     3961 attacgtaaa tgaaaactag aggtgtaaat atggcgaatt tggtttatag tcctaaagac
     4021 attttacaac aagaattcaa aacaaaaatg cgcggctatg atcccgttga agttgatgaa
     4081 ttcttagaca atatcatcaa agattatgaa acttacagca aagaactcct tgctttacaa
     4141 gaagaaaacg atcgattgag tgcaaaagta gctcaattat ctaaaactca aggagcagct
     4201 caaacacgcg tgcaacaaac agaggcacca aagagcgcag cagtgaccaa ttttgatatc
     4261 ttaaaacgct tgtcaaatct tgaacgtgaa gtatttggta aaaagcttga tcaacaagca
     4321 agtgcagtca aaccagcaca acctaatcca aataactaca caaatgcaga cacaagctta
     4381 gatgataatg aaaaaacacg tcaattttaa tttttgatta atgaatttgc ggttttcggg
     4441 taatcgcggt ctgacttgat caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc
     4501 tgtagtgttc gtgcttagcg aaaccataag ctaaggtact ctttggagta acggcaggaa
     4561 aacaagctaa ggcttcggct atgcttaagt atccttgaaa gtgccacagt gacgaagcgg
     4621 ctggggaaac ccatccggtg gaacgcggta aacccctcga gcgagcaacc caaacaatgg
     4681 taggggcgct ttttgtctgg aagaagaaca ggacaaaaag gcagtataac tgcagataga
     4741 tggttaccgc tttgtttttt ctccctggaa aagcaaagta cagaacatgg cttatagaaa
     4801 accgcaaaca tcagggaatt aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa
     4861 acatttgaaa gctatttttc aatacttcag atgaatcgtt cttattacaa aacaaacttt
     4921 tgtatcaccg gttattcgga atttgagtaa aagtgtgggg taggactgat gtcccacctc
     4981 ctttttttat aaataaggag attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca
     5041 actgcggcaa gcgggctaga agcactggtt ggtaaagaat taagagatct tggaatcgaa
     5101 tgccaagttg agaacggacg tgcacgtttt gaaggaacaa tggaaacgat cgctacagct
     5161 aatttatggt tacgtacagc agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat
     5221 acgtttgatg aattgtttga aaacgtaaaa gcattgcctt gggaagattt tcttccacta
     5281 gatgcagctt ttcctgtagc tggaaaatcg atcaagtcga aactatatag cacacctgat
     5341 tgccaagcga tcacaaaaaa agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca
     5401 gcttctgtac cactgacgga aactggcgca cattttcagt tggaagtagc tttgctgaaa
     5461 gaccatgtga tggtaactct ggatacgaca ggaccaagtt tattcaaacg tggttatcgc
     5521 ttagaaaaag gtggagcacc attgaaagaa aacatggcag cagcacttat tatgttgaca
     5581 aattggcgaa aagaccgtcc attttatgat cctgtatgtg gttcaggtac gctttgtatt
     5641 gaagcagcct taataggcca taatatcgca ccaggattca atcgtgagtt tgcttgcgaa
     5701 tcttgggatt ggttcagtca agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa
     5761 gcagattatg atattgaatt agatattact ggttcagata tcaatggacg gatgattgaa
     5821 attgccaaag ccaatgcaga agaaatcgga ttaggctctt cgattacctt taagcaacaa
     5881 gcagtcaaag acttcaaaac ggataaagaa tacggggtca tcgtggctaa tccgccttac
     5941 ggagaacgtc taggagaaga agaaactgtt cggaaattat ataaagaaat gggcgatgta
     6001 ttccgtccat taaaaacatg gagtaaatat atcttgacta gcgacttagc gtttgaggaa
     6061 ttttacggac aaaaagcaac caaaaaaaga aaattgtaca atggtgcatt gcgtacggat
     6121 ctattccagt attggggaac tcgtccgcct agaaaaaaag agaaagacga ataatcttac
     6181 taattgattc attcaattgg atgtttggag gaatagggaa tgaaagaaaa agagttttta
     6241 caagaagtaa aagaaatcaa tcttctacaa caagctttag gaatcctcga ttgggataca
     6301 caaacaggta tgcctgaaaa agctagtgca taccgaagtg aagtagacag ctatctttac
     6361 agcatttatt tttcaaaaaa aatcggtcca aaaatcaaag aagccattcg ctatttttct
     6421 gaacatccgg aagaattgtc ggaagttgga actgccgttt ttgccaaagt aaaagaagag
     6481 tatgatttgg aacatcgcgt accggaagat cttatgagaa aatatacagc tgccgtttct
     6541 tcagcacacg gacaatggca aaaagctaga gaaacacaac aattttctga ttttcaacac
     6601 gcattgaatc aaaatattga attaacaaaa cagttgatac cttactggag aaaagatgaa
     6661 aagacgaatt atgatgtttt gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt
     6721 gataaggtat ttgatcagct taaagaagga attctttcga tccgccgtac gctccaagaa
     6781 aaagggactg aacctgaaac agacttttta aatcggagaa tgacgaaaaa acaacagaag
     6841 agatttgtag tgaaagtaat cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat
     6901 gacactgtcc acccatttat gactggaatt aatccgaatg atgcacgaat cacgacacga
     6961 tggaacgaaa cagatttcaa aatggctgtt tttggagtga tccacgaggc tggtcatggt
     7021 atgtatgaac aagacatcga tgataaatat gcgtatacac cggtttatga aggtacatca
     7081 atgggtatcc atgaatcaca atcgttgttt aacgagatca ttattggaag tagtcgcagc
     7141 ttctggaaaa agcaatatcc tttctttcaa gaatgtgcag aaggaacatt cgatgatatt
     7201 ccttttgaca aattttatcg ttcattgaac catacaaaat caagtttggt tcgtatcgag
     7261 gcagacagtt tgacttaccc tctccatatc atcattcgtt atgaaatcga aaaaatgatt
     7321 ttcaatgaag gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa atatgaagag
     7381 tatctgggaa tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt tcattggtca
     7441 ggtgcaagct ttggttattt tccctcttat gcattagggt acatgtatgc ggcacaatta
     7501 cttcattctt tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga cgattattct
     7561 ccaatcaaaa actggatgtc agagcatatc catcaatatg gtgcatctag aaaaccaaat
     7621 caattaatca tggatgcaac acatgaaccg cttaatccgc aatacctgat cgactatatg
     7681 aaagacctgt attttaatgt atatcaggta aaataaaata caatttacaa agatagctga
     7741 aattcatcta atctatagat gttttcagct atttacttaa gaaagaagtg gaacgaatgg
     7801 ataaattaca agtgatcgct gattatagta tggaccaatt agcgcatgat caaacaggac
     7861 atgggagcga tcataccaaa agagttgtca agttagctga aaaaattttg gagacggaac
     7921 cgcaggcaga ccgctttgtg acgcttgcag cagcttacct acatgatacg attgatgata
     7981 aagtcgttaa aaatgagaac gaggcaaagc aacagcttcg tgtattcctt cgctctttgc
     8041 caatagtaga ggaacagatc tcgatgattt tttccattat cgaaaatatg tccttttcta
     8101 aaaatctctc tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa gacgctgatc
     8161 gtatcgaagc attaggagca atcggcattt tgcgtactgc ttatttcggc ggtggacatg
     8221 ggcacccgat ttttgatcct gaactgtatc cgcaaacatt caaggacaaa gaaaattacc
     8281 gaaaaggcac gacagtgatc aatcattttt atgaaaaact gttcttgctg tctgataaaa
     8341 tgaatactga ttacggatat gaagaagcca aacgaagaga aaccttcatg cgtgaattct
     8401 tagaagaatt ttttctggaa tggtctattg acgatgaaaa ttttactcca gacaattgga
     8461 tcaaaaataa caaaaaatga tggaggttgt gacagaagtg gtcagctcca agatatttcg
     8521 gatcattttc cgtaaaagtt acttctggaa cactgtttac caagaggtcg tgagcctgcc
     8581 gttcagctcc gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat
     8641 tgtaacttgt gattcggaat tggcaggaga acaccgttta tt
//
LOCUS       DASJSZ010000102         8287 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.106, whole genome shotgun sequence.
ACCESSION   DASJSZ010000102 DASJSZ010000000
VERSION     DASJSZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.106"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            476..1339
                     /locus_tag="U4B03_002451"
     CDS             476..1339
                     /locus_tag="U4B03_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2069259.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="U4B03_002452"
     CDS             complement(1468..3090)
                     /locus_tag="U4B03_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2069260.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="U4B03_002453"
     CDS             3339..3860
                     /locus_tag="U4B03_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN2069261.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="U4B03_002454"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="U4B03_002454"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEN2069262.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="U4B03_002455"
     CDS             5941..6759
                     /locus_tag="U4B03_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEN2069263.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="U4B03_002456"
     CDS             complement(6862..7059)
                     /locus_tag="U4B03_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069264.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="U4B03_002457"
     CDS             7228..7806
                     /locus_tag="U4B03_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN2069265.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="U4B03_002458"
     CDS             7901..8038
                     /locus_tag="U4B03_002458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069266.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2494 a         1486 c         1634 g         2673 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgtt
//
LOCUS       DASJSZ010000103         8232 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.107, whole genome shotgun sequence.
ACCESSION   DASJSZ010000103 DASJSZ010000000
VERSION     DASJSZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.107"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="U4B03_002459"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="U4B03_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEN2069267.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="U4B03_002460"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="U4B03_002460"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEN2069268.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="U4B03_002461"
     CDS             2594..3379
                     /locus_tag="U4B03_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2069269.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="U4B03_002462"
     CDS             3392..4243
                     /locus_tag="U4B03_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEN2069270.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="U4B03_002463"
     CDS             complement(4367..4819)
                     /locus_tag="U4B03_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069271.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5284..5652)
                     /locus_tag="U4B03_002464"
     CDS             complement(5284..5652)
                     /locus_tag="U4B03_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEN2069272.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5802..6284
                     /locus_tag="U4B03_002465"
     CDS             5802..6284
                     /locus_tag="U4B03_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEN2069273.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6321..7331
                     /locus_tag="U4B03_002466"
     CDS             6321..7331
                     /locus_tag="U4B03_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEN2069274.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7446..8159)
                     /locus_tag="U4B03_002467"
     CDS             complement(7446..8159)
                     /locus_tag="U4B03_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069275.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2785 a         1337 c         1668 g         2442 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaat atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg
     5041 tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt
     5101 ggaaaattca aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa
     5161 aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga
     5221 acaaaacctg tataagctga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa
     5281 gctttatcgc caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg
     5341 ttcaatcaat tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata
     5401 tacgtagcct gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc
     5461 gatagaaaaa tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa
     5521 cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc
     5581 aaaaaggtct ttgatctttt gttgatgttc tttttctttg atctgattga tgatcggctg
     5641 aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca
     5701 atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg
     5761 ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga
     5821 aattcttaca cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg
     5881 aagatgcttt ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat
     5941 tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg
     6001 atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca
     6061 tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga
     6121 ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta
     6181 gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg
     6241 agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc
     6301 atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg
     6361 gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt
     6421 taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag
     6481 ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag
     6541 atgaaaatat ccaaatgatc tttccgcctt ggggaggaga attattgatt gagattctcg
     6601 attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta
     6661 cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga accaacctta
     6721 ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa
     6781 caagagaagg agaatcgatt ctacagcaac cttctgaaaa gtatcaaaaa gagtggcccc
     6841 atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca
     6901 gcggagatgc agtaaggatg aagggacgat tattaggtgg ctgtattgat acgatcagtc
     6961 atttaatcgg taccccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa
     7021 agccgatctt gtggtttcta gaaaattgtg agctatcgtc agttgattta agaagatcac
     7081 ttgttcagat gaaactggca ggatggtttg atggcagctc ggggatcatg ttcggtagaa
     7141 gtgcagcaga ggatttgtta gattataaaa tgaaggatgt atatgaagat ctgtcaaagg
     7201 aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat
     7261 tgatcaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa
     7321 cgtttattta aaaaattttt tcctgccttg gagtttatca aagaccaaca aatctaaata
     7381 gaaaagaaaa tgaaagaagc tgacagtatt ttgccagctt ctttttgtat gatctattag
     7441 aaattttaat tatccataac tatcacattg atcagctgtt tcacatcatt tggattacct
     7501 gtaggaatag ctactacaga tgcattatca ataatttctt gagttgtttc tctaatgaac
     7561 gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg
     7621 attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg
     7681 agcataacca catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt
     7741 cctgcaggat aatgaataac atgtagttcg aaatcacccg atgtatctct aaaagtatta
     7801 cctctaattg ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac
     7861 ggatctattg actgagaaga ctgtgaggaa gttgtgtcta ttgttgaaga agaaatattt
     7921 gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt
     7981 gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg
     8041 gtttgtttga ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga
     8101 gacgtacatc ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata
     8161 taaaatacct cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt
     8221 aaaaccttgt ct
//
LOCUS       DASJSZ010000104         8058 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.108, whole genome shotgun sequence.
ACCESSION   DASJSZ010000104 DASJSZ010000000
VERSION     DASJSZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.108"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(36..884)
                     /locus_tag="U4B03_002468"
     CDS             complement(36..884)
                     /locus_tag="U4B03_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2069276.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1108..3048)
                     /gene="thrS"
                     /locus_tag="U4B03_002469"
     CDS             complement(1108..3048)
                     /gene="thrS"
                     /locus_tag="U4B03_002469"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEN2069277.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3385..3564)
                     /locus_tag="U4B03_002470"
     CDS             complement(3385..3564)
                     /locus_tag="U4B03_002470"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEN2069278.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3611..5434)
                     /gene="cadA"
                     /locus_tag="U4B03_002471"
     CDS             complement(3611..5434)
                     /gene="cadA"
                     /locus_tag="U4B03_002471"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN2069279.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5712..7991
                     /locus_tag="U4B03_002472"
     CDS             5712..7991
                     /locus_tag="U4B03_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2069280.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2367 a         1710 c         1455 g         2526 t
ORIGIN      
        1 attctaaaaa acttatataa aaaatagtaa ggtttttatt tcgaatccat aaaatattct
       61 tggattgctt ctcgtgtttt tttcactttt tcgctaagct tttcgttttt agccatatag
      121 aaataaaaaa tgatatcgat cgtagcaaat tgggccaaaa gtgaattagt tgccgcactt
      181 cgattcgtga tttcttttgg tctagatgtt tgtacgagta catcggctat cttgctaagc
      241 gggttatttc caaattgcgt caacgcaatg atttcgatat tcatgctttt cgccagctcg
      301 gctaatgcga ctacatctgc acttctacct gagtttgaaa caagccaaag cactttcttt
      361 ttctcattgc tgacaagttg cgggagcaag acgtggatat ctttttcaaa aatgattggc
      421 ttaccgacac gagaccattt ttgcagaata tcttctacta ctaaagaaga ggcgccaacc
      481 ccatataaaa aaacagtctc tgccgattcc aaacactcta cgactttttg gatcgtttct
      541 tcttctagaa tatctactgt ttcttttatc gctacttgtg cattgttacc aagtttgttt
      601 ttgatagagt aaaaggactc atttgcttct atatcactaa aaccttcatc gattccagtc
      661 tgtagttctg ttgaaatcgt gattttgaag tccgtcagac tttgaaaacc gatttttttg
      721 gaaaaacgta caactgtcgg cgcactcaac ccggaagctt ctgctaattc attagctgtc
      781 attgtaggga ccttctcaat attaccgata atgtagttgg ccaacttttt ttctgctgaa
      841 gaataatctt ccatcatact ttcaatacga ctaattacgc tcataagttc ctccaccctt
      901 ttaatgatag tctatgtaag tataccatat cagaccctta ttcacggtcg tatactggat
      961 aaaaaacaaa aaaacaatcc gcccaacaga atatcttatc ccatcaatcc cacagattct
     1021 attgagcatc tgtgtctttg aggaatacct gttatctatt gttcggattg ctttctatct
     1081 actacttttt tacaggaaat ttacctatta gttttctctg ctgtaatttt tgacttctgc
     1141 ttccatagca tcaataaaga tattcaaatc ttctacagct gtttctttac ttccataacg
     1201 acgtacgttc actgttgcat cttccaattc cttgtctcca acaactaatt ggtaagggat
     1261 cttttgtgtt tgagaggcac gtatcttata gcccattttt tcattacgat catcaacttc
     1321 tacacgcaac cccttcattt gcaaacgttc tttgatttca taagcataat cgccatgtgc
     1381 atcaacagaa acagggatga ttgttgcttg tataggtgcc aaccatgttg gaaaagcgcc
     1441 tttgtatact tctgttaaat aagcgacaaa acgttccatt gtagaaacaa ttccgcggtg
     1501 gataactact ggacgatgcg tattttcccc gtcttctcca acatatgtca aatcaaagcg
     1561 ctctggcaat aagaagtcta attgtatagt tgataatgtt tcttctgtac caagagctgt
     1621 tttgacttga acatctagtt ttggtccgta aaaggctgcc tcgccttctg cttcaaagta
     1681 atctagttct aattcatcca tagcggcttt cagcattgct tgggcttttt cccacattgc
     1741 gtcatcatca aaatatttat ctgtattgtt tggatcgcga tagctcaagc ggaaacgata
     1801 atctgtaata ttgaagtcag catatactgc caccatcaat tcaagcgtac gtttaaattc
     1861 atctttgatt tgatcaggac gaacaaatgt atgaccatca ttcaacgtca tttctcgtac
     1921 acgttgtaaa cctgacaaag cacctgattt ttcatagcga tgcatcattc ctaattcagc
     1981 gatacgaatt ggtaattcac gataagaatg gatcgtgttt ttgtagacca tcatgtgatg
     2041 agggcagttc attgggcgaa gaacaagcat ttcgccatct cccatatcca ttggcgggaa
     2101 catgtcttct tgataatgat cccagtgtcc agatgttttg tacaactcaa catctcccat
     2161 tattggtgta tatacatgtt ggtaacctag actaacttct ttgtctacga tataacgttc
     2221 gatcgtgcgt cggattgttg ccccttttgg aagccagaat ggcaaaccag aacctacttc
     2281 tggggaaacc atgaacaagt caagttcttt tcctagttta cgatggtctc tttcttttgc
     2341 ttcttcacgg agacggatat attctttcaa agctttcttg tcaaaaaagg ctgtcccgta
     2401 tacacgttgc atcatttgat tgtttgagtt tccacgccaa taagcacctg ccactgataa
     2461 taatttaaat acttgtatac ggccagtgga tggaacatgt ggtccacgac acaaatcgat
     2521 gaattcgcct tgttgataag cagtaatcac ttcttcttct ggcaattcac taatcagttc
     2581 tactttgtac ggatcacttg caaaaatttc taatgcttcc tgttttgaaa gaacacggcg
     2641 ttcgatcggc agattttctt tgacgatttt catcatttct gcttcgattg ccggcaaatc
     2701 ttctgcagtt acttggtttt gtccattatc tgtatcgtag taaaaccctg aatcgatagc
     2761 tggtccaacg ccaaagtgga tatttggata gaggcgacgc atggcttggg ccatcaaatg
     2821 agctgctgaa tgacggacta agccaagtgc atcttcatgg tctggtgtga tgatctcaat
     2881 acttgcatct tcctcgattg gacgagtcaa atcaacgagt tttccgttca ctttaccagc
     2941 taaagctttt tttgctaagc tttttgaaat gctttcagca attgccaatg ttgttgttcc
     3001 agactcaaat tctttgactg caccatctgg aaaagtaatt tttatcataa tagtttcctc
     3061 cttgaaatgt tttgattgat cagatcatca agctgaaaaa caaaaaaatc ctagcatctc
     3121 ttgaaaagag atactaggac gaaaagttcg tggttccacc taatgttcaa acgcaaatgc
     3181 gttcctcaag cgttgtaacg gtccgaccgc ccttgcttca acaagggttt cgaaagtggt
     3241 catcctttgg ttttctcctg gaaaagtttc agcctagctt ttcctctctt atcgaagact
     3301 ctcaaattca gttggctttc tcatcaatca attatgtgtt tatttaacca ctaataaata
     3361 gaaaaatcaa gtgggaaact aaacttattt tttccattcc ccatttacac cgtatgttcc
     3421 ttttttcatc tcattgatga tgacatggat gtgttctttt ggtgcctgcg tgtttttatg
     3481 aacagcttcc gtgatatctt tcatcatatt ttctaattgt tccggactgc gaccttctac
     3541 taattcgaca tgtacgaatg gcataatgct ctctcctctt tctgatcgtt cttttttcaa
     3601 ctcgattcta tcatactatg tttgtcttgg gtactgaatc aggaaaaatg gctggatctt
     3661 tttttgctct caaggcactc aaaatagaga ccgtatccac gacttcttgc aaaacagctc
     3721 cgaataatgc ggggattagt cccgtacttg caattaacat caaaaaaata caaataaaga
     3781 tgccaatcag tacagactgt ttcgctattc tcatcgtatc tcttgaaatc actactgctt
     3841 ttgctacacg caagagatca tctttcaaga tgaccgcatc tgcactttca ctagcagctg
     3901 tagaaccatg agcccccatt gcaataccga tatctgcagc agcaagtgct ggtgcgtcat
     3961 tcacgccgtc tcctaccatc gcaacaggtc tttgatcttt agggatactt tctaaaatgg
     4021 tcagtttatc ttgaggcaag ctacgggcat gtacagaatc taattggact ttttctgcaa
     4081 tcgtttgagc aatggcagat ccatccccgg tgatcatcat caaatttttc acatcaagtt
     4141 ttcttagctc cttgattgtc tttgctgctt ctgggcgaat ctgatccgaa aataggatcg
     4201 ttcctgcaaa agatcgatca attgttacat agattgctgt attggacgtt tctatttctt
     4261 cagctgaaac aaaagaggca cgtccaatct ttacttcatg attattgact actgcttgga
     4321 ttccttgacc ggtcacttct ttcagttgac taacaggata cagttggctg tttttcacgt
     4381 tgttcactaa cgaacgagct aagatatggc tagattcctg ttctgcacta gctgcataca
     4441 gaagtagctc ttcttttttt attccattga ctggctgaat cgcctctact tccaatatcc
     4501 ctttggtaag cgttcctgtt ttatcaaaag caatcgtttt tgtacgtgcg agcttttcaa
     4561 tcgttgttcc agtttttaca acgatcccgt ttttacttga acggctcatt cctgcaacta
     4621 aagcaatggg cgcagctaga atcagtggac aaggtgatgc cactaccagt acctcagcaa
     4681 atcgtaaagg gtcttttgag aaccaccaag aaataccacc gatcaaataa gcaatcaacg
     4741 tgaaagggac tgcataacga tcagctaaac ggacaaatcg ggcaggcttt tcttttgatt
     4801 cttcgactag tttaataatc atttgatact ggctgtctgc ggcacgtttt tcagcgatca
     4861 tttggatcga tccgtctcca tttaaagacc ctgacataat ctgatctcca ggtttcttgg
     4921 tttccgggcg ggactcacct gtcaaagaag attcatcgac cgaagattct cctttggtca
     4981 ctcgaccatc tgcgggtaca agttcgccag gcttgactag taactggtca tcaatattta
     5041 tttcatcgat tactatatcc tgcaattttc cttctttgaa tcgatgggca attcttgggg
     5101 tgttatctaa caaagactgc agttctttac ccgctcgttt attcgcgtaa tcctctagac
     5161 tatctccgcc cgtaagcatg atcaatatca tcaaactagc ccagtattct ttgacggcta
     5221 atgttgcaag gattgcggta atcgctaata catctacgcc atatcttcct gaacgtaacg
     5281 tcttgatcat ttccaaaaac atcaagaagg cgagaatccc tccagtaaat gcgacaatcg
     5341 caaatgcaag ggccggctgg tgaaaaacaa attcacttaa taaagctatc ccaccaataa
     5401 gcaaagtaag taaaaatttt ttgaaatgtg tcattgtttt ttctcctctt ttatgtgact
     5461 tagagaggtt agtaataaat ttccagctgc ctctttcatc ttaagcatag tccttgacta
     5521 gcacttttta aaacaatagg ccttaattga aaacaactat cactatcagc gtttccaaaa
     5581 ttcatcagtt ctacaaacaa aaatattcag caaggattac tttttcgatt catcagttaa
     5641 aaagtacctt ttttcgaaaa actatgctat acttttaatt cgttatttac ggcggaagga
     5701 gcaaacatgc gatgaatgag aaacaactag aacaaacgca cgtggatgaa acgatcgaac
     5761 taatccagat cgaacaaaat atgctggata caaaacaaca agaattgact caggaaatga
     5821 atcattcgac aaaagacaca gctaatcaca agattcgagc tggttccaat gaagcttttt
     5881 atgaatctgc tgttgaatat cgccaacatg agcaggaatt gcttttgaaa taccatactc
     5941 ttgaagcaca gcaaaaacgc ctgcaaacgt tgtctgtcat gaaagatagt ccttattttg
     6001 cccgtatcga ctttaaagaa gaccgtgaaa cagaaacatt atatctaggt atcgcttctt
     6061 taagagatct ggcagaagaa acaatcgtta ttgactggcg cgcaccaatt gccaatcttt
     6121 attatgaagg tgagattggt cccgcatttt atgaaactga aacagataga atagatgtcg
     6181 atctgcagtt gaaacgacaa tttaaaatcc aagaaggacg tattttatct atggtagata
     6241 cgtcggaagt aatcaatgat gactttttgc tagagatatt ggatgaatca tcaagcaacc
     6301 atatgaaaaa catcgtctct acgatacaaa aagcacaaaa tgcaatcatt cgggacacaa
     6361 caagtaaagt aatgctgatt gaaggaatcg ccggaagtgg aaagacatcc gctttgcttc
     6421 aacgtattgc ttttctgttg tatcataatc gcaagtggct cgaggctgaa aacgttcttc
     6481 tattttcacc caaccatttg ttttctgatt acatctcgac tgtattgcct tcattaggtg
     6541 aaagcggcgt gcctacacag acattcaaaa attatttggt gcagctgctg ccagagtttt
     6601 ctttactaga tgaacagcaa caagaaagtg gttttttgct aggggaaaaa gatccgatac
     6661 aagtgatgaa aagcggattg accttagtcg atcagattga tcgctatatc caaggcatca
     6721 caaactacgg tcccttgttc cgtgatatga aaataaatgg ccgtacgatc ctatcaaagg
     6781 aatcgattcg ccaatggtat aaagaaacaa atgaacaatt acctttacat catagacttt
     6841 ctctattaca aacgaagctc ctgaaaaaat taggcggtct gcaaaaagac gaaacacgtc
     6901 agcaatgggt caaagatctg gcggaagaac agctccaaga actatacgca acagatccaa
     6961 atcttgaata tacagaacaa aaggagcgtt cattacgtaa aaaactggcc aaccaaatcg
     7021 tacgtaaaag attccgtcga atccatcgag ggatcatgaa ttatcaattt gtcaatataa
     7081 cgaaacaata tctccacttt ctacaaactg tgccactttc tcttctaaca cgacataaaa
     7141 tcagcgagac caggtggcag gaacatttga caactgttcg tacacacttg cgtcaaagag
     7201 aattgcgtca agaggatgct gtactattct ttttactgat gcgccgaaca tatccagtga
     7261 cggttacaca caaagctcgc tatatcttta ttgatgaaat gcaggatttc gcaccatcac
     7321 aagtggcatt gctgcaagcc ttgttcccaa aagcaaattt gactttctgt ggagatctaa
     7381 atcaaaatgt ctttggaaat gaaacgatcg ttggatcatt ggaagacctt ttcgtggacc
     7441 gtgaagtcac tcgtttccaa ttaacgacaa gctatcgctc cacaaaagaa atcactgatt
     7501 ttgctaatca ttttattgtg aaagaacatc aagtagaaac aacagcaaga aaaggagacc
     7561 ttcctttgct catccaagga acaacagatg atatcaaatt gaattggtta gcagatacca
     7621 ttcgaacgac tcagggaaaa gcccggtatt ggcgtacagc aatcatagct aaaacacaag
     7681 aagaatgcga agctctttat gctcaactgc cagacgagtt gaaacaacaa gtccagctga
     7741 ttttaaatga gagtgacttt atgaagcgtt ccattatttt acttccagcc tttttagcaa
     7801 aaggtctgga gtttgaccga gttttcttgt gggatgtaga tgcagaacat ttttcttctc
     7861 agcaagacaa attgatcctt tacaccatgt gtacacgtgc gatgcatgaa ttgacccttt
     7921 ttacctccaa taatgaaagt cctttgttaa acaatatgga tatttcatct tatgagaaaa
     7981 aagtactgta agtaaaaaga caactgctat cattaatgtt agcagttgtc tttttattta
     8041 caataataat tgcagaaa
//
LOCUS       DASJSZ010000105         8057 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.109, whole genome shotgun sequence.
ACCESSION   DASJSZ010000105 DASJSZ010000000
VERSION     DASJSZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.109"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..452
                     /locus_tag="U4B03_002473"
     CDS             <1..452
                     /locus_tag="U4B03_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069281.1"
                     /translation="PFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYKLPQVFISLI
                     VLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLSDKLGIKGRG
                     NFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            465..2483
                     /locus_tag="U4B03_002474"
     CDS             465..2483
                     /locus_tag="U4B03_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEN2069282.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2543..3259)
                     /locus_tag="U4B03_002475"
     CDS             complement(2543..3259)
                     /locus_tag="U4B03_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN2069283.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(3486..4028)
                     /locus_tag="U4B03_002476"
     CDS             complement(3486..4028)
                     /locus_tag="U4B03_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2069284.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(4033..4260)
                     /locus_tag="U4B03_002477"
     CDS             complement(4033..4260)
                     /locus_tag="U4B03_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2069285.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(4339..6219)
                     /locus_tag="U4B03_002478"
     CDS             complement(4339..6219)
                     /locus_tag="U4B03_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN2069286.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(6408..7022)
                     /locus_tag="U4B03_002479"
     CDS             complement(6408..7022)
                     /locus_tag="U4B03_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2069287.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            7164..7724
                     /locus_tag="U4B03_002480"
     CDS             7164..7724
                     /locus_tag="U4B03_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2069288.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         2448 a         1582 c         1322 g         2705 t
ORIGIN      
        1 acccgtttgc attacagaat ggacagagta ttttatggat tttttattta tttttaatgg
       61 gttatggttt gaaagaatgg aactggaaag aaaaggtccg ctataagctt cctcaagtat
      121 ttatcagctt gattgtttta ttcggtttga ttattttgat gacacaaatt tcactcatca
      181 tcagaggaaa tacagataca gcgctgaggt tttctaaacc atattctctc ttcgcaatgt
      241 attatacttt ctcttcgttt ttatttttag aaatgctatc tgataaatta ggtataaaag
      301 gaagaggaaa ttttcttgct gtatttttga ttgtcactca agttatatgt aatttccctt
      361 tagtttctta tcgcgtatct acatttatga aacgtgaatt tccaaactca gggaaggcat
      421 ggctgataaa tatcgggcag ttttttattt gatatttatt agcagtgtcc ctcctagtta
      481 tctttttatt ttatatacaa aaaatccgtt tcattcatcg tttcatcgaa tacttcgctt
      541 ttgattcgat tgaacaatta actcagcgtt ggaaaaaaag aattcattgg ctttttgttc
      601 ataaaagtta ttttttagtt gcagcatttt tttactgctt taccttcgtg cagattttcg
      661 ttttagaacc aaaagaaggt tggaaagaaa ctatccaggt tgccttaaaa atatttactc
      721 agcgacaagc acccatgatc ctgacaatca tcattattat gggatttttc ttcttactat
      781 tgttgatttc caatcgtttt tggtacgcct tgactgctac cttgatcatt aacttactgc
      841 tcactatttc gacagtaatc aaaatggaaa tgcgggaaga gcctgtcttt ccttcagatt
      901 taaaaatgct gaccggattg tcagaactat tatcgatggt tagcccagtc ttattaatcg
      961 tgggcggatt gatccttctc ctattattga ttactagtat tattgtacaa cgtagactac
     1021 aaaaacagta ttccttgaag attcattgga aacgtcgatt tatcagtatc gctgtattat
     1081 taggatgttt ttcgggtgtc tttttcatta atcacaaaaa ttcgccttct ttcttgctgt
     1141 ttaatttatt taaagtaaac aagactttct ttaaccagca aggcgccgta aaagataacg
     1201 gaccagtcat tcaattccta aacaatatcg acattaaaat catggaagaa cctgctggct
     1261 attcgaaaga aaaaattaat ggaataatga aaaagtacaa cacaaaggca gatgaaatca
     1321 accaaacaag aagtgactgg ttaaacaatc agacgatcgt tctcaattta agtgaaagct
     1381 tcagtgatcc tgcacgtgtc cctaatctga cagtacctac agaccctatt ccttacatca
     1441 aaagcattat aacagaagga acaggaggat tgatgttatc cgtcggttat ggtggcggaa
     1501 cagctaacat ggaatgggaa ggactgacag gcttgagtat cagtaacctg tctgcttcgt
     1561 tagttacacc ttatacccag ctagtggagc gtcagaaaat aagtccaaat attaccgatc
     1621 tattcgatga aaaaatagcc atccatcctt ttacagctgc tctttatcgc agaaaacaag
     1681 tgtttgataa atttggtttt gacaggttct attatttaga cagtccggat aaattaactt
     1741 atacggataa aatagaacaa agcccacgca tatcggatga ttcagcatac aaagaaacct
     1801 tgaaagcttt acaaagcaat acggaaacgt cacaattcat ccaattatcc actatgcaaa
     1861 accatatgcc ttatgatgat tactatagtg aattgaacta cactgctgaa ggaactgctg
     1921 tcattcctaa tagaagaaaa gaattacaaa cttatatgca aggccttcac tataccgata
     1981 cagcagtaaa aaaattcatc caagaaatcg acaaaatcga taaaccgatt acctttgttt
     2041 tttatggtga ccatttaccc gcaatctata gtgggaatga ttcgaaaaaa tatggattag
     2101 aacagcacca gacagattat ttcatttata acaacaagta tagtcgtgaa caagcagcta
     2161 cgttaaataa agaagttgtt gctcctaata actttggtgc tatggctctt gagcatgcga
     2221 atatcaaagt aactccttac tatgcgttgt tgacagaagt atctaatcat ctacctgcat
     2281 taacaattga tcctacagaa agcttgtcta atcgttttaa tggaaaacag gtctttgtta
     2341 cagaggatag tcaaattatc caagaaaaaa atttaactga gaaacaaaaa gaaattctag
     2401 aagattatcg tctgatacaa tatgatcttg ttgcaggtaa ccagtacagt gcaacatggg
     2461 cgacacaaaa aataaaagac taacaaataa actgagcaag gggttgtgat aaaagacatg
     2521 tcacaacccc ttgtttatct cattaatttg cttgtccata aacgatttga ttaaagccat
     2581 ttttcaattc attattcgct gtctcgttga agtaaagata attatcggga ttcccctgat
     2641 tttgttgtgt caccaatact tttggctgat tttgctgaaa cccgaataga tagacgtgct
     2701 ttttcagata tggttctgtc tctgcatcgg aatagacggc tgtgatttga tagtctgctt
     2761 gacctgtccc actttctgac cattctgcag aaacaggcac tccaccaata gccatcttcc
     2821 actccccgtt caaaatagct tgagggactt taagaccata taagtccaca ctcatttgat
     2881 tcgtataact tttgtattgt tgtcccattg tttggcccca tttggtcata aatgcctcta
     2941 attgtgcgga tttgtctgca ttccacagtg tttgttcaac ttgttcgctg ctttctgaag
     3001 aggaagatga agcctctaca gaagaacttg aagtagatgt ttgagttgtt tcagtcgttt
     3061 tggaagactc tgacgaagta gaggtatctg ctgtttctgt acttgctgca gttgacttgc
     3121 tctctgtgct agttgttgta gtagttgcaa ctgttgcttt cgctgtttct tttgttgatt
     3181 cttttttcga ttcattcccg ttggtacaac cagctaacaa aaaagctaga ccaattgtta
     3241 ctaaaatcct ttttttcaat taaaaaactc ctttcgatta ctcccacttc tttattctat
     3301 caaataagca atgtgatttt attgattttt cgtattttct catcaatctt tcaaatcttc
     3361 taaaaactga gaagcacccg ctgatgttgt ctgtaataca tcagcgggtg cttcttttat
     3421 tttaatcttc gtgattttcc aatttacctc acgctttata tttgtatttg aagaaacata
     3481 gacttttagt catcatcgtc ttcctcatcc aaatcttgtc ggacagtttt tccttggagt
     3541 ctttgtactt ggtagatgat ttctttattg aaacttaaaa gttcaacaag caattgttgc
     3601 aagccatatt tttcttcttt ttcagctaat ttgattgccc gtgtgataaa gacattttgg
     3661 aaatcaaaat cttttgcaaa gttttccaac attgttgagg ctggtaaata tttgttttga
     3721 ccatgttcgt aaatttttcc ataacgcatg acttcttcta aagtagacgg aacgatttca
     3781 ttttcaatca ctaactcatg agagatttta ttgaaccatc tcgcttcttg atcagcatat
     3841 tctgaaaaaa gggtacttaa ttcttgcgaa gatgtaccca aaacatacaa ttgtgcttgg
     3901 atcaatttca tttcttctaa tttaaaattt gctaatacat gccccaccat ggcgccagca
     3961 gttggtttat gatggtcgat atctgcttgt tttgtttctt gaacaaattt ttcttccact
     4021 atattcattg atttacgcct cttttacttt tgctgattgg acttcataac ctaatttttc
     4081 cacaactgca gctaattgtt cagggtggac ttcatctgga tcaaagtttg ctttgatttt
     4141 gctcgcatta aacaaaactt tcgtattgct taccccaggc tgttgagaaa ccgcatgttc
     4201 gattttttgc atacagcttg gacaagctag tgtatctaat tgcataataa tttttttcat
     4261 cgtagttctt ccttcctttt tcttgatagt cttattctag attacagctt ccataaaaaa
     4321 attgattctt atcaagtttt acgatttttc ttctttgctt tcgtgtccaa aatacaataa
     4381 acgcatcgcg ttaaagataa cgatcaagat actagcttcg tgaacgaaca tcccactagc
     4441 catgtaaata aacccggcaa ataagccgtt gagtaaaaat aagacgacac caatggcaat
     4501 cactacattt tcacgggtat ttaaaacagt tttcttagcc aagcggtagg catgaaccaa
     4561 cgattcaaaa ttagattgca tcaagacaac gtcactcgtt tcgatagcaa catccgttcc
     4621 tgatcccata gcaatcccga tatcagccgc agccaaagag ggagaatcat tgattccatc
     4681 tccaataaaa gcaacgcgca atccttgttc ttggtatttt ttcacaaatt gaaccttttc
     4741 gtctggtaat aattcagcat ggacttcatc aatgcctaac atatcagcta cataatcagc
     4801 agtcatctga ttatcacctg tcagcatcac taactgtttt gctcctgctt gtttcaactt
     4861 agctaattgt tcagcgactt ctggtcgaat cacatctgac acacctatga tttgagtgat
     4921 ttttttgtcg attgctacaa taatgaccga gctgcctgtt tgttgtaatt gatgtaaatc
     4981 ttgtacttgc tttggtgtga gagaaagttt gtgagcggca atcaatttgg tgttgcccgc
     5041 acaaactgta tgaccatcaa tctcagctgt gattccttgt cccttgattg tctgattatc
     5101 tttgactgct aattgttgaa aatcaatgtc tttctcctga gcataatgga tcacggcgcg
     5161 ccccagagga tgatcggata acgtttctaa tcgagccact aaagctaagc tgtccccttc
     5221 atcctctgta tagttcttga tagtggatac agcagtagtt cctttggtca atgtaccagt
     5281 tttatcaaag acaaaagtat cgattttgga aaaagtatcc attgcttcac cgcctttgac
     5341 taagatgccc cgtttcgctc cattaccgat tcctgctaca ttcgaaacag gcgcaccaat
     5401 gactaaagcg ccaggacaac caagaaccaa aacagtgatt gctaaacgaa aatcacgact
     5461 aatcagacca acaaccaacg caataagtaa aacggttgga gtataatact gagaaaatcg
     5521 atcaatgaat ttttccgcat gggatttggt atcttgtgct tcttctacta attcgataat
     5581 tttggcaaag gtcgtatcat ctccgacctt agttgcttca atcgtcacgt agccatctgc
     5641 taaaatcgtt ccagagaaga cttcatctcc actgattttg acgacttgtc gtgattcacc
     5701 agtcactgcg gcttcatccg catacccttt cccgtcaatg actcgtccgt ccacaggaat
     5761 agctccacca gtttttacta agacgatatc gccttcttcc acgtcatcga tttccacttc
     5821 ttcagtcgtt ccatcttctt ggacaacact agcagtagat ggggccatct cggtcaaagc
     5881 cttgattgac tcccgcgttt tggtcaatgt tttttgttcc agatagctac caaacaaaaa
     5941 caagaatgta acgatagctg actccttgta ttctccgatc ataaaggcac caagcacagc
     6001 aatagtaacg agtaaatcaa ttgagataac tttgactttc actgcctgat acgcatggat
     6061 cataataggt atgaccgcaa taatagaagc tatggctaga gcaatgttat agaacaagac
     6121 aaaaccagta aattttccga taaaaccaaa aataatcaac acagctgata aggcggtgat
     6181 atgatttcga tgctcttgga tccacttttg taatttcatc tttctccctc ttttcctttc
     6241 attcttacga gactaagtat agatacttat ccatcataaa aaattgacca ctatcaagtt
     6301 tagtcaaaaa ggcggttttc ttttggtaga atacactaca aaaaatagtt agcaagaaga
     6361 ctcactaaaa gtgatcttgc caactattgt cgtttctatc gaaattttca ttgaatgatt
     6421 ttcctgtaca gcatacatcg gagatctatt tctttatcta agatttttat cttgctaatt
     6481 ttttccttta gtctttcaaa tccacgtcgt tcatagaatt gatacgtaca gccactatct
     6541 gtaaaaagga atatttcttt tcctttctct cttttttcaa attctgttag caacttggta
     6601 ccgatgcctt ttgttttcaa atcaggattt gcagctaaaa aaatgatctc tccatctggc
     6661 gaatgcttct ttaaatatgc tgcaagcatt tgttcatttg ctgtctcgta agctccttta
     6721 ctttctttag caaatatatt ttgtaaaaaa tcgaatatct taacatatat ttgctgtcca
     6781 aaagaacgat gtttcttttt ttctcctttt atctccgcca gcaaaacacc tgccaactcg
     6841 tcctctgcat acaaagcaag aatttgagtt gctcgattca actccatata caaaaaatat
     6901 cttccgtaaa gattcagcag aagttcattt tccatatatc tctcaaagtt cattccttca
     6961 acagcaaaat agatagcttt tttgtaatct ttcttttcta agggtttaaa agttagtttc
     7021 atcatttcgc ctcctcaact tcaatgattg tgttatactt acattataat agacacagtg
     7081 tctactatct ataatcaatc atctataagt ttgtcgatta agcgacagct ttgctttgat
     7141 atgtcggata aggagatagg taaatgaaaa agacagatag acgcgtcaaa aaaacagaaa
     7201 aagctttggc tgaaacacta tccacattat tagtaaataa aaagattcaa gcaatcacta
     7261 tacgcgaact aacagaaacg gcagatgtac atcgttcgac tttttataca cattataagg
     7321 atatctatga tctatatgat caattagaaa gcaacttttt tcgggatcta aatgaaattc
     7381 tctcttatga tccagcccat agctatgagg aactatacac tcgcttgatt gactatgttt
     7441 acgcaaatac ttttttatat cgcttattta tcctcaacca tggtgatatt tcgtttcaga
     7501 agaaagttaa acaagttatt gaagaaaact atctgaacat atggctatat gaagacaaga
     7561 aaacgattat cacagaagaa atgagattct ttactacgta ccacgtccaa ggttgtctat
     7621 ccgttctcaa tcggtgggta gaaagcaatt acaagtactc caaaaaagaa ttattagaac
     7681 tcttacagaa actgaatgat cacatagaga aaatcatgcc ttgaataaaa acatactaag
     7741 cttagttaag aagaaccgta accttctcgt ataacaactg aatcttattt agccaaattg
     7801 tttactgaag attttttgag taaaatttct ataattccta cttattgaaa agtagtttgt
     7861 agtgctgctt ctccctctct aagtgatata tcttgttgta ccaatcaaat tcacctcatc
     7921 attaataaat tacttttagg ctaatttatt acaacttata ggttgaagct gtaaccaatt
     7981 tatagtcata ttatattttt atcatccagt ttattgaatt cttctcgttt acctcaattc
     8041 attttcaagg ggaatca
//
LOCUS       DASJSZ010000106         7808 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.110, whole genome shotgun sequence.
ACCESSION   DASJSZ010000106 DASJSZ010000000
VERSION     DASJSZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.110"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..372
                     /locus_tag="U4B03_002481"
     CDS             <1..372
                     /locus_tag="U4B03_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2069289.1"
                     /translation="ADNKVPGDPMSVMALTIAKPADCKLDIVKISDVKGIMETSPNDN
                     YMILIKGPKEAFELVQNLPEIKEINFGGVSKKSESKQYGKAVFLTPEELEATKKILDQ
                     EVKIFVQQVPSSSIENVDFSK"
     gene            386..1135
                     /locus_tag="U4B03_002482"
     CDS             386..1135
                     /locus_tag="U4B03_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2069290.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1135..1965
                     /locus_tag="U4B03_002483"
     CDS             1135..1965
                     /locus_tag="U4B03_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2069291.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            1965..2612
                     /locus_tag="U4B03_002484"
     CDS             1965..2612
                     /locus_tag="U4B03_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2069292.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(2710..3486)
                     /locus_tag="U4B03_002485"
     CDS             complement(2710..3486)
                     /locus_tag="U4B03_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2069293.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            3630..4589
                     /locus_tag="U4B03_002486"
     CDS             3630..4589
                     /locus_tag="U4B03_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2069294.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4706..5014
                     /locus_tag="U4B03_002487"
     CDS             4706..5014
                     /locus_tag="U4B03_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN2069295.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            5041..5340
                     /locus_tag="U4B03_002488"
     CDS             5041..5340
                     /locus_tag="U4B03_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HEN2069296.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            5514..6065
                     /locus_tag="U4B03_002489"
     CDS             5514..6065
                     /locus_tag="U4B03_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEN2069297.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            6211..6504
                     /locus_tag="U4B03_002490"
     CDS             6211..6504
                     /locus_tag="U4B03_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069298.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            6497..6793
                     /locus_tag="U4B03_002491"
     CDS             6497..6793
                     /locus_tag="U4B03_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069299.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            6864..>7808
                     /locus_tag="U4B03_002492"
     CDS             6864..>7808
                     /locus_tag="U4B03_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN2069300.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVY"
BASE COUNT         2744 a         1150 c         1471 g         2443 t
ORIGIN      
        1 gctgataaca aagttccagg agatccaatg tctgtgatgg ctttgacaat tgctaaacct
       61 gctgattgca aattagacat tgttaaaatt agtgatgtta aagggattat ggaaacatcc
      121 ccaaatgata attacatgat tttaattaaa ggacctaagg aagcttttga attagttcaa
      181 aatttaccag aaattaaaga aattaatttt ggcggtgtgt ctaagaaaag cgagtccaaa
      241 caatatggaa aggcagtctt tttaactcct gaagaattag aagccacaaa aaaaattctt
      301 gatcaagaag tgaaaatatt tgtgcaacaa gtcccatctt caagtattga aaatgtagat
      361 tttagtaagt aaggggggaa aataaatgtt attaaaagcc attttattag gtttagttgg
      421 tgtggtagct gttattgact ccaggttaat tggtcgacaa aatattggta gaccactaat
      481 tttgagtaca cttgccggac ttgtgctagg tgacttgcat acagggattg ttcttggggc
      541 atctttagaa ttaatttcta tgggattcgt ctcaattggg gcggctggac ctcctaatat
      601 gcaacttggt agtattattg ccactgcttt tgcaatttta actaatagta gtacagaaac
      661 agccttaaca atagctatcc cagtggctgt agctggtgaa tttatgagta ttatcatgcg
      721 catgataatt gctcaatttg gacatgctgc agataaagcc attgaaaata ataaatttag
      781 aaaagctcaa attattcata tttattggtc atttattttt aatgcttttg tttactttat
      841 tccaatcttt ttaacagtat attttggtgc tgacgttgtg gcagatttag tagataaaat
      901 tccagaaatg attacagacg ccttgactgt ctctggaaat atgctttccg cattagggtt
      961 tgcaatgcta ttaagcacaa tgttgagtaa aaaattatat ccttacttta tttttggatt
     1021 ctttattgtt gcctattcag gtctttcact aattggcgtg acaatttttg cagctctgat
     1081 tgcctttgta atggatcaag ttaaatatgg aaaaagagaa gaggcgcatg gttaatggaa
     1141 aatagtacag aaaaaaaacg tttaatcaca aaaaaagatc ttaataagat gtgtcttcgt
     1201 tcatggacaa tcatgtgttc gtggaattat gaaagacaaa tgcatatggg atttatgtat
     1261 gggatggcgc caattttaaa taaagtttat aaaaatgatg aagagaaaaa aagagaagct
     1321 tatactcgtc atatggcatt ttttaactgt acgcctcaaa tgacttcatt tattatggga
     1381 ctttgtgctt ctatggaaga acaaaatgct caagcagaag aaggaaactt taatactgaa
     1441 tctatctcca tgattaaaac cagtttaatg ggtccttttg ctggaattgg tgattcattt
     1501 ttccaaggta ctttaagaat tattacattt gggattggat tgtcttttgc taagcaaggt
     1561 agtattttag gaccgatttt agctgttggc ctttttgcta taccttctat tttagctgtt
     1621 ttttacggcg gtaaattagg atatgagtct ggcaataaat accttactaa attgtatcaa
     1681 gaaggtctta tggatcgagt aatgcaattt gcttctatca ttggtttggc tgtagtaggt
     1741 ggaatggtgg ctagcatggt gccaattgtc acccctttaa aattcactac agggggaaca
     1801 gaacttgttt tacaagactt acttgattcc attattcctg gattattacc acttgtatgt
     1861 actttgggaa tatataaatt gattaagaaa aatgttagta caaatatgct cttattaggt
     1921 tgtattttgg cagggtttgt tttaagtata ataggtatct tataatgatt aaggctctaa
     1981 tttttgatat ggacggagtt ttaattgata gcgagattat gtatcaaaag tgggtattta
     2041 attttttaaa acaagaaaat ttaagctatc ctttgtattc ttatcaaaaa aagattggaa
     2101 ctacagcaag tatttttgat gatatagaaa agtacaatga gggatgtaac agtttagaat
     2161 tgaagggaaa atttactaaa tattggaaat cgcaaaagat tgattacttg gatatttttc
     2221 ctaatatact aattgaacag cttgaatggt taaaaaagaa aaaaataaaa atggcaattg
     2281 cttcttccag tccaatggat actataaatg aagttgttac cacttgtaaa atagagtcat
     2341 attttgattg tttaattagc ggaagagatt tgccagaaag taaacctaat cctactatat
     2401 tccttaaagc ttcagaacag ctcagaattc cagttgaaga atgtctggtg atagaagatt
     2461 cttataatgg tataaaagct ggtaaaagag caaatatgaa ggttcttgca attaaagata
     2521 aacgtttttc tcaagatgta agtctagcag atgatgttat ttacgatatt aaatatctgc
     2581 gacaagaagt acaggtgaaa tttggaatat aaataagttt tttgatgtat ttagagaata
     2641 ttagtaaaaa taaaaatcat gcccaaatct aatcaaaatt gattgctttg ggtatgattt
     2701 cttttaatat caatctatta ctttaggatc tgaatcattt ttatgcaccg tatctaaagt
     2761 gatattttct gtgacttcaa taacataact aattctatca caacgaactg tggattgaga
     2821 ataatgaaat ggttgattat ttttatcttt gccaaccttt tctaaaataa atagcggatc
     2881 tcctactata cattgcaata agtcggaaag ctcaggtgtt gcagataaac cgtttatttc
     2941 cataatagag ctcgtagctt tgacatcata gtcatcttct agaagtttat ataatgaagt
     3001 atattggcta cctttttcaa ataaagttgg aaatttttct tctgatgcga aaaaattatc
     3061 aattcccatc ggggcaccgt cttcccacat taaacgttta atattgtaaa ctctatcagt
     3121 tacattgatt tgtaatttat tagctaaaaa ctcatcagcc tttataattt tagaatgtaa
     3181 aacagtatat ctaattttat gtcctcgggt agaaagagaa tcgctaaatc catgaactcc
     3241 agctaatttt aaactttctt ttttttgagt tacaaaggtc cctttccccc ttaatcgttc
     3301 aactgttcca ctttctgcta aaagtttaaa agcttttcga atggtaatct tacttactcc
     3361 ataaatttta cataattctt cttctgtagg taattgctct cctggaagat attttttttc
     3421 acgaatatcg ttacgtatag aagcttctac ttgtaaatat aatggtctat tatcttgaaa
     3481 attcatagta cactccctaa aaagttatac ttcattatat cacaatttat taaaacgtcg
     3541 attgtaaaat taagctagac aactaaaaag atcatttgtg ctacactcaa aatagttccg
     3601 accaaagaat taataaggag tgagtacaaa tgacttacac ccatcttaca acagacgaac
     3661 tagtaatcat agagtcttat ttcaatatcg agaaacctgt cgctcttgtc gcacaaagtt
     3721 taaatcgctc tagacaaacc atttataatg tttattcgtt tctcaaacaa ggaaaaaaag
     3781 tgctagatta ttaccgtcaa tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg
     3841 ttttatctga tgaacaatca acatatgttc aagaaaaggt agctcaagga tggtcacccg
     3901 atgtgattat cggccgtggc gagtttccaa ttgattgttc tgttcgtaca ctctatcgga
     3961 tgtttaaaaa aggccttttt gacacacagt cgttacctat gaaggggaaa cgtaaaccga
     4021 ataatcatca agaaaagcgt ggaaaacagg cctttcgcag aacgattcat gaacggaaca
     4081 gtctctatcc acacctgact aaagaatttg gtcatctcga agtcgacacc attgtgggag
     4141 ttaaacacaa gagcgctgtt attacattgg ttgagtgttt atccaaggtt attattactt
     4201 tgaaaccgac aggcagacga gccgaagata tcgaaaacag gttaaatgat tggttcaaat
     4261 ccatgcctaa acacttgttt aaatccatga catttgattg tggaaaagaa ttttccaact
     4321 ggcgatctat aagtaactcg aacgatattg ccatcttttt cgcagatcca ggaacacctt
     4381 cgcaaagagg gctaaatgag cactcaaacg gcttgcttag acgagatggc ttacctaaaa
     4441 caatggactt tagggacacg gatgaaacct ttattcaatc cgttgcctcg aaaagaaacc
     4501 atattccaag aaaatcatta aattatcgaa caccattgga agtatttttg agctgcatag
     4561 ataaagacat attgtctagc ttaatttgac aaatcagata gtataaaatg aatcaaattg
     4621 aatatcaaat atatattata tccatgaagc ccaatcttct ttaatagaag attgggctat
     4681 ttttatggca aaggagggaa aagaaatgtt tcaaaataaa ggacaacgtt atgtcacaaa
     4741 gggagtaatg gacagtttgc cggtggaatt acaagcttta tgttggaatc taatcgatca
     4801 aaacgttcaa aagcaatttc atttagacta tcttcaaatc tttgaatttt ctacggaaaa
     4861 aggaaatcaa aagttggttc atcgtcaaga agaaccagaa gcaagaaaag agtatctgat
     4921 ttcacctaag cttcgtttga aatcagtgtc tcaaaaaata tgggtcattg attcgggtga
     4981 gtatcaaacg atgttattac cagaagaata ctgatatcta aaagaaagga acgaaaggac
     5041 ttgttagaaa cggctatcga agtactggaa aagtgtgcac agctagtcac tactcctgaa
     5101 gagtggggct atgaatccgt tacgatggaa aaagaggaaa ttgaaatggg tatgcttcct
     5161 gaagatgtta ctctaccacg tttagtgatg acacatcttt atatttattg cgcaccagaa
     5221 gatggaaaag actatgtagt ttatttcatt acagatatta ccagccagcg agaatttgtt
     5281 cgtgggttac tggtagaagg acgattggtt tggagtcaga ttggaggaac gaatgaataa
     5341 gatcgattat ttgagaatga ttgcgaaatg tttagaagat atccttacag cgattcatca
     5401 aattctgaag aaaaaaggag aaagaatatg aaatattttc aactagtatt gacacttata
     5461 acggcaatgg tgattgaatt gcttgaacaa aaattagcat aggagaaaaa gagatgattg
     5521 aaaaagaatt agtgaccaca caaacagaag tttggcgaga tcctcatcat acccatcgct
     5581 atgttttcaa acgacaatgg gcgatcaaag gaaaagaagc ggaagataaa ttagcggtag
     5641 tcattactat tcgtccaaca gatacagaac catttacgaa tgatttgagt atgttattaa
     5701 tcgaaaagaa tatccgacaa ttaggattta ctggttttat tgctgtgaat cttttttcgt
     5761 atacaaaagc gaaaagtcct cgggtatttc ctcgtggaaa tgatgaacaa tctttggaaa
     5821 tattaacaac tgttttcaca gaaaagaaaa tcaacaccat tatttttgct tgtggttcga
     5881 ttacagcgac gagtcaagtg gcttatgaac aagcgaagac catttacgat caattgagtg
     5941 cgaaacaaaa aaagatgacc aaagtattga taaatgcaga aggtaagtta tcccacccac
     6001 taagtattca tgttcgtaat gaatggcaat tggcagatag tcaattatta tttcaagaaa
     6061 aataacattt tattttctta tgttacttat aagtagtttt acatgctttt tgactttaaa
     6121 aatacatgtg atgatacatg ttatatgaat tatcaatgta cccagtaata tgctattttc
     6181 attatgtaaa aaataacagg gggtgtgtag atggagattg atgaaacttg tttgagtaac
     6241 tcttataaaa gtatgaatga cttttttgag gaaatggaac tggattcaga attgctctat
     6301 caattttatc ttgcttttcg agggcaacag atttcttttc caatgaaaat gtacgatcgt
     6361 gagttagtta gaaaaagaat cgaaaacatg atagaacagg aaaaaactgt agatatccgt
     6421 caattgacag aagtttatgg tttctctgca agatggattc aaaaagtaat taaacaaaaa
     6481 gaaaggagac gagtacatgg ctaagctgat tgtattaggt ggtttaattt acggtggata
     6541 ttggttatat aaatatatgc ttgcgcatcc gaatatagat aaaggaaaag taattaagac
     6601 agttggggta atcatagttg gactcatagc tctaactaag aaaaataata ctaatagccc
     6661 aaaaataatg agaacttatc gctgtagagg atgtggaagg gaaatttcta ctagttttaa
     6721 tccatctgaa agccctcaag gaagagatcg ttgtcatgct ggctatcgac attcttggga
     6781 aaggattaac taaatgaaca ccattgagat taattgactg ataaaataag tacgcgaggg
     6841 agaaattgaa agggggaaat aaaatgaaaa aatggttgtt attactaatt ggaatgggga
     6901 ttttattcac tggttgtaca tctgagaata cgaggactaa agagactgta gaaactgata
     6961 caacaattga ggctaaaaaa agtaaaacgc aatcaactga acaaactaca acagaaatga
     7021 aacaatctgt ggaagcaagt acaagtgaaa gttcaatcat tgaatcttct tctgaagagg
     7081 ttaaaaatac tttgtgggat agtgagaaat ctactcaact agcaattttt gtcccacaat
     7141 ggggaaagac attaggtcaa gaatataaaa gctacaatcc tcagaacaat gtaagtctgt
     7201 atggtacgcc tttaccaagt gcagttattg atggaaattg gaaaatggca atcaatgaag
     7261 caccagttac cgtacaatgg tcagaagatg gaactggtca tgcagatttc aatttagtag
     7321 cagtttattc tgatgtagaa actggagaat atttagggca gcatgtatat ttctttggct
     7381 ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa tcaaggcaat gataaaaact
     7441 acatgtactt caatgaaact cagaaccaac agttaaaaca aggtttcatt gatattgtga
     7501 atggacaaac tcctcaaact ccagtagtag aagaatctac tcaacaagca acaactacaa
     7561 cgacacaatc tacaagtagc tcagcaattt caagttggga agctgctaaa gaaactgtac
     7621 taaataataa agctttatgg ttgtatgatt ctcctgataa aaatgcatca aatactaatt
     7681 taattagcac aaatgaatat atgaaagatc taatgcaaga tgaaaatgga agctactatt
     7741 caattaatat tgtaacggat gataatggaa atacaacaat ggctggtgga aaacaattcc
     7801 gtgtttat
//
LOCUS       DASJSZ010000107         7757 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.111, whole genome shotgun sequence.
ACCESSION   DASJSZ010000107 DASJSZ010000000
VERSION     DASJSZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.111"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..107
                     /locus_tag="U4B03_002493"
     CDS             <1..107
                     /locus_tag="U4B03_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HEN2069301.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="U4B03_002494"
     CDS             complement(176..904)
                     /locus_tag="U4B03_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2069302.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="U4B03_002495"
     CDS             complement(917..2197)
                     /locus_tag="U4B03_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2069303.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="U4B03_002496"
     CDS             complement(2338..2940)
                     /locus_tag="U4B03_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN2069304.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="U4B03_002497"
     CDS             3185..3391
                     /locus_tag="U4B03_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069305.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="U4B03_002498"
     CDS             3703..4422
                     /locus_tag="U4B03_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2069306.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="U4B03_002499"
     CDS             complement(4503..5186)
                     /locus_tag="U4B03_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEN2069307.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="U4B03_002500"
     CDS             5405..7591
                     /locus_tag="U4B03_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN2069308.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2782 a         1307 c         1465 g         2203 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
     7741 gaattttgtc acacctc
//
LOCUS       DASJSZ010000108         7659 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.112, whole genome shotgun sequence.
ACCESSION   DASJSZ010000108 DASJSZ010000000
VERSION     DASJSZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.112"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            113..307
                     /locus_tag="U4B03_002501"
     CDS             113..307
                     /locus_tag="U4B03_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069309.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            576..875
                     /locus_tag="U4B03_002502"
     CDS             576..875
                     /locus_tag="U4B03_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069310.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(972..1406)
                     /locus_tag="U4B03_002503"
     CDS             complement(972..1406)
                     /locus_tag="U4B03_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2069311.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1638..2348)
                     /locus_tag="U4B03_002504"
     CDS             complement(1638..2348)
                     /locus_tag="U4B03_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN2069312.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2338..3198)
                     /locus_tag="U4B03_002505"
     CDS             complement(2338..3198)
                     /locus_tag="U4B03_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEN2069313.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3203..3841)
                     /locus_tag="U4B03_002506"
     CDS             complement(3203..3841)
                     /locus_tag="U4B03_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEN2069314.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3856..4155)
                     /locus_tag="U4B03_002507"
     CDS             complement(3856..4155)
                     /locus_tag="U4B03_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2069315.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4198..5682)
                     /locus_tag="U4B03_002508"
     CDS             complement(4198..5682)
                     /locus_tag="U4B03_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN2069316.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5702..6163)
                     /locus_tag="U4B03_002509"
     CDS             complement(5702..6163)
                     /locus_tag="U4B03_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2069317.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6181..7248)
                     /gene="ulaG"
                     /locus_tag="U4B03_002510"
     CDS             complement(6181..7248)
                     /gene="ulaG"
                     /locus_tag="U4B03_002510"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEN2069318.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7466..>7659
                     /locus_tag="U4B03_002511"
     CDS             7466..>7659
                     /locus_tag="U4B03_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2069319.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYN"
BASE COUNT         2345 a         1613 c         1214 g         2487 t
ORIGIN      
        1 aaaaactttc aaattgattc tttttattta tcataaagat atagatacat aatcattttc
       61 acttacatat atttgaatat taactaaaaa cagcaagaaa gaagaggatc aaatgtatta
      121 tgtagaagta aaaacaaaag gcgtcaaaaa caaacaatat gtgaaaggta tcagtaacga
      181 atatccttta cttggttcat ggaaagaagc tgctcctttt tccaaaccat gtgccataaa
      241 aataaagaac gagctagaaa aagaactgac ttgtggtaaa gctgttgtag atatcatcga
      301 aaagtaaatc caatgtatag aaaggaagat cacacatgaa aagcgacgta caaattgcta
      361 agattataga attagaagaa gaagacgcag tgttgacaat tgaaaaaaaa taaatgtttg
      421 tataccatat gattatttca atatgtatcc tggaattgga gatctcgtag agctatatca
      481 agatgaatca aatatacttg tcattcatca tgaaatatag agacttataa aattgtttat
      541 ttttataatg tatcagtata gaaaggaaat aaaacatgga ttatactgtt gaatttgaca
      601 gtgaactact taagcaattt ccacaagaag aacatttcat gtataaagaa gcaatttccc
      661 acaaagtgac agagtatttg gagcataatt tttttcgtat aaagccagtg cgtactcgtt
      721 cggcaaaagg aatatacgag atgaaagtca aaattaataa aaaaaattat cgcatagcat
      781 ttgctttaaa aaaaaatcat gtgttcattt tttatattag ttcgaatttg caaaaaattg
      841 tatttgataa agaggtgagt aaaaaaataa aatagctgag cattggagga tagattgagt
      901 gatactaagt ctatcctctt gtttttctaa agaactaagc tagaattttt tcttttgtac
      961 gttattatat gttatttgtc attaccaaaa agcacctttt ttcttctagt atctatgaca
     1021 taagaaatac cacccatttc ctttacttga acaaatccta ctgattcata aaattttttt
     1081 gttttctcag tatcatctgt caataattcg atttggactg gtccataact agtgatcttc
     1141 tccaaagctt cattcaaaag cactttgcct attccttggc gctgatacct cggatgaatg
     1201 aggatatctt gaataaatgc gatcgtcgca aaatcagtga tccaacgaac gattccgata
     1261 atcttccctt ccccattacg ctttatcaga gattcagatt gttcaaatgc tctagctaac
     1321 cttgcagtgt cttttgtgta attcgtccaa ccaacgctct tatataattc agaaatctca
     1381 tcgatcataa gggaatcaat attcattttt ttcttccttt cttatacaat tctccctcta
     1441 ataatacagt tctttgacta tttaatctat cacatataac tctcctgtaa agattctctc
     1501 gattccttaa aattcctctt tccttttaga atgcatactt tttgataaat aaaaaggttg
     1561 tgataaattt tcttcacaac ctctgtttct ctctatcaag tgttatcttt taattttcta
     1621 cccttaccct agcatctcta ttgcccgtag tatgcatact ttccatgttt gcgaaaataa
     1681 tgtttatcta gtaaatattg cgggatttca catatattgg ggttgatcag ctcgttttct
     1741 ttggccatca gacaaatttc atctaagatc aaactgtttt ctactgcttt cataggtgaa
     1801 tccccccatg tgaacggtcc atgtccatag acaagtactc cagggacttc gtttggatct
     1861 aattttcttt ccttaaacgt ctcgacaatc acattccctg tatgaacttc gtaagcttct
     1921 cttacttctt ctttcgttaa ttggcgtgta caaggaacct taccataaaa agcatcagca
     1981 tgagtcgtgc cataagctgg caaatcgcgt cctgcttgcg cccacatgac ggagtgcgtg
     2041 gaatgagtat gagtaatagc agttatatct tcaaaagttt gatacaaaac aacatgtgtg
     2101 gggagatctg aagatggttt taagctatct tcttctatta tatttcccga caaatctgtt
     2161 accaccattt gatctgcttg catgcattca tatctgacac cgcttggctt aatgacgatg
     2221 actcctagct cacggtccac ttcactcaca ttgccccaag tcaatttgac taatccgtat
     2281 cgcggcaatg ccagatttgc ttcaaggaca cgttgcttca ttgctgcaat tttttcttca
     2341 caagacatca tagccagcct ccttcaattt agaacttaag tagtcttttg cctgctggat
     2401 ttctcttcga aagtcttggc ttttttcact ccacatttca atcagaaatg ttccatcata
     2461 atctagacgt ttcaaatgtc ttaataatcc aagaaaatca acacaaccgc taccaaaagg
     2521 aacatcacga aactggcctg aatattcttt tgttacaggg taagtatctt ttaagtggat
     2581 agcagtgata taatcgatcc ctttctcaag ttctcgagcc ggattatttt ccggccatgc
     2641 ggacaaattg cctaggtctg gatatacttg tagaaaaggt gaatgaatct gttctttgat
     2701 ttctaaaaat tttgaaatgg agttcataaa tggatcatcc atgatttcca cggaaagaat
     2761 gatgccatat ttagaagctt cctttacgct ttcttttatt ccttcgataa agtcttctct
     2821 ggaattcatt gatttctctt cataataaac atcgtatcca gctatttgga ttactttgac
     2881 gcttagatta tgcgccaaat ggattgcttt tttcattagt tgtttcgctg cttttttctt
     2941 ttctggatca gcagaaccaa acggaaatcg ccgatgcgca cttaaacaaa tcgaatggat
     3001 acgtacaccg gagctgaaca tctcttctct cagctcagct atcttttctt cagaccaatc
     3061 taatcgtgct aaccgctcat ccgtctcatc aatcgacatc tcgataaaat cgaagtccat
     3121 ttcttttaca agggaaaaac gttctttcca tgaaatatct ttgggtaacg ctttttcata
     3181 aatcccaatt tgagccattt ccttaccccc agatacgaat gatctcatct ttgaagtttt
     3241 gtgcggcctt catcggatca tttgctgctg taataccacg accagtgata aaggtataca
     3301 catcgatccc ttcaaataag tgtaatgtgt gtggatttaa tccaccagtt acagaaacat
     3361 taaagcctag ttcaatcagt tttttgatct tcgataaatc tttttcaccc caagtttcac
     3421 cagataacag cgcatcacgg ctttgatggt agattacctg ccggataccg atgttatgcc
     3481 aatccatggc ttgttcaaaa gtccaattgc cgtagagttc tacttgcaat tcacctattt
     3541 ctttttgtgc tgcctccata gttggaatcg ttgcacaaca aatgactgtc atccagtctg
     3601 ctcctgcttt tgcaacatta ctcgccactg ttcctccagc gtctgcacat ttcgtatcag
     3661 cgactagttt tttatctggg aacatactgc gaatacagcg aatcgctttt tgtccttcct
     3721 gtaagcaaag aattgtgccc acttcaataa tatctactac atcaccaact ttcataacat
     3781 ctcctaatgc atgttctaag tcattgtgat ctaacgcaat ttgcaaattc ggcctgctca
     3841 tcttagttcc ttcctttact tgtttttttg gtaccacttt gtttcagcaa atttttcact
     3901 gatttctttg actgacatca catttttgat accgataact gtaactcctt ttttctctgc
     3961 ctgagtaaac atattcaaaa aattgactgg cgtaaaaact acatcgtatt ggccagctga
     4021 actttttccc tctgaaatgg cacaatgatg gattttggta attggtacgt ttagttcttt
     4081 cattgctttt tccacacttc tcatcatcat caagcttgtt cctgatccat ttgcacacga
     4141 tactaaaatt ctcatcttaa tccctccatt atgagtgttg tctaatcaga ttttctttta
     4201 tactctctgc ttttctgcat atgtgtcata gtcttctaca atcaaaaaat aaccatcagg
     4261 atttttccga tattgtaact gcggaatagc caataaacca atgacaacta cggcaattcc
     4321 agcataacct aggactttca tgattactgt aaacaatggc caagctgttg cccagtcgaa
     4381 catcccgata taaccaccgt atgttgacat cccaatccat gtagcgaata atgctgaacc
     4441 gcctacttgt atgattcctg aaagaaacgg gaacaggcaa gcggctttga atccacctcg
     4501 gttattggca taaactgcta taacggcatt atcaaagaac aacggaatga aaccggctat
     4561 gacgatcgtt ggtgatttca ataaaatcag taaaccaatc atcaaaaact gtcccaaagc
     4621 accaaacaag aacccaatcg ttaccgcatt tggcgaacca aagccaaaag tagcagctac
     4681 gtcaattcct ggaacagctc ctggtaaaat cgtgtttgag attccttgga aagattgagt
     4741 tagttcatcg acgaatgtcc gaacacctaa ttgcaagatc gccagatata ccgcaaaatt
     4801 taaagaagtc gtcataatgt agaataagaa actctgccct tcttgcatga attgcgcttg
     4861 aatcaaataa tctttgccta gtacaatcag aatgatccca aagaaaaata gcatgagtaa
     4921 cgaagtagca accatattct cattgaaaat cgacaagaat cctggtaatt tgatatcttc
     4981 aatttttttg tcttgcttga ttgatctatt tttcttttca tcccgctttt tcatccactc
     5041 agctaatttt gcaaatataa agattccgaa catttgctga tgtgccactg caaatccggc
     5101 accttctgtc aattcctgcg tgattcctac tgttaagtta gaagctactg tccaataaca
     5161 acctaaaatc agtcccataa aaatcaatgc ttgtatccta cctagatcag gaaagcaaaa
     5221 cagtaaaatc caaaaagcgg tagctgcttg ttgaacctga acgtttccgg tagtgaaaac
     5281 ggaacgcagt ttcgtatatt tctgaaaacg gactaataag atatttaaaa caaatgcaat
     5341 cagtaataaa atcatcacat ctccaaaggt acggccaaat gtctccatta atccagcatc
     5401 cacagcattt tgtccaaaat aaggatctgt aaccatggca tccaaattga accgttcctt
     5461 tagtccaacc aagattggcc gaaaattatt gaccaaacca ccagagccaa cagtcaaaat
     5521 aaagtaaccg accgttgcct ttaagaatcc agcgatgcat tcatgaagcg ggcgtttcag
     5581 caaaatatag cctaataaca cgataaaacc aatcaggtat gctggctgcg tcaagatatt
     5641 tgttgcaaaa tacgtccaaa tgcttaacaa aaattccccc atcttttttc tcctctatca
     5701 attaattata ttctgccatc acaacttggt aatcatcgat ggagttcgta gagatgagac
     5761 gctcaaccaa tccatctgtc attaataatt cggataattt ttggatattt tctagatgtt
     5821 cttctggatc ttttgctgcg agtgtaaaaa acaaactagc cgttttctcc tcattctccc
     5881 cttcactttc aaaacggatt ggctgtttca acttggtaaa tgaaatagct gttccaaaaa
     5941 caccatcact tttttctgaa gaatgcggca ttgctacttg aggtgcgatc acaatataag
     6001 gaccgtattt ttctacacaa tggatgatct cctccacata cgtttcatca ataatttcct
     6061 tatcgatcaa tacaagacag ctttcggcga tagcttcacg ccaatccgtg atttctttat
     6121 ctgggtaatg aatcaattga tggtcaaaaa agtatttcag caagaaactc cccttctttc
     6181 ttataaaaac gacggaaacg gtgtatcatt ttcaatcgag aagacatctt caaatcctcg
     6241 ataaaaatta aactcaagat catctttgtt tgtcggatag atgaatttcc cgcctacttg
     6301 ccagatataa ggtttaaatc catacttcag gcgatccttt ttgaatttcc aaatttcagt
     6361 gatctctttt ggatcagcca taaaattaga ccaaatatcg tagtggacag gaatcactac
     6421 ttttgcattc aatgactccg ccatgcgcag catatcaaca gaagtaactt tgtctgttat
     6481 ccctcgtggg ttttcaccat aggcccctaa acagacatcg attttgtgtt cattaccatg
     6541 tttggcaaat aaattggagt aatgtgaatc tcctgcatga taaagattcc ctcctgatgt
     6601 ttcgaataaa tagtttactg caatttgatc catctcctga ggcatttttc cttttaatac
     6661 ctcttctgga tcttcacaag tgaccaacgc tgtccgatca aacgcttcta aagctactac
     6721 attgatgtct ttgattttga ctgcatcacc tggctttact gtaatcgttt tttcttcagg
     6781 aattccccat ttttgccacg tgcgaaccac ttcttttggc ccaatgaatg ttgcttcagg
     6841 acaattttga tgaactgctg cggctgtatg gatatctaga tgatcggaat ggatatgtgt
     6901 aacgattaaa gcgtctacat tttttatcgc aaaaggatcg attacgaaag gctgagtacg
     6961 taaattaggc tgcatatttt gaacgccact catacgcatc atctgatggc cttttttcat
     7021 catgccattc ccatgactac gttttcccgt cccacaccat agatcacata aaatattcgt
     7081 tccttcatga cttttcagcc aaattcccgt acagcccagc caccacatag caaaagttcc
     7141 aggatccact acttctgttt cgatttcttc attcaagtat gtgccccact caggaaatgt
     7201 ttctactaac cacttttctt ttgttacttc attgacagtt ttactcattt gcttgctccc
     7261 cccatatcgt tgttccaact tacaagtaaa tattaatgtg tatcagcatc catgaaaacc
     7321 cttttatatg accgtttgtt ttttatatga ttttctttca gtaatcaatg attgtttcag
     7381 tataataaag acagatatga atgtttaaat gaacaactag tcaaataaat ccatggatca
     7441 agatgtccct caggaggcaa aagtcatgaa aaactcactt gtcaatattg aaaaacgtca
     7501 aaaagaaatt ttggaactgc ttcagcagac tgaacaacta tcaactttcg aattagctga
     7561 atcccttgat gtatctatta gtacgatacg aagagattta aatttattag aagaaaaaaa
     7621 cgagatcata cgcaattacg gttattgttc ttataatta
//
LOCUS       DASJSZ010000109         7603 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.113, whole genome shotgun sequence.
ACCESSION   DASJSZ010000109 DASJSZ010000000
VERSION     DASJSZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.113"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..222
                     /locus_tag="U4B03_002512"
     CDS             <1..222
                     /locus_tag="U4B03_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2069320.1"
                     /translation="AHQLKLNIPEDLKIIGYDGTSFIEKFFPQLTTIRQPIDEIASLI
                     VDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(392..1818)
                     /locus_tag="U4B03_002513"
     CDS             complement(join(392..1245,1245..1818))
                     /locus_tag="U4B03_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HEN2069321.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSPVQFRKTTVTVV"
     gene            2061..2516
                     /gene="tnpA"
                     /locus_tag="U4B03_002514"
     CDS             2061..2516
                     /gene="tnpA"
                     /locus_tag="U4B03_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEN2069322.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2781..2879
                     /locus_tag="U4B03_002515"
                     /pseudo
     CDS             2781..2879
                     /locus_tag="U4B03_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(3562..4380)
                     /locus_tag="U4B03_002516"
     CDS             complement(3562..4380)
                     /locus_tag="U4B03_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2069323.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4541..5230
                     /locus_tag="U4B03_002517"
     CDS             4541..5230
                     /locus_tag="U4B03_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN2069324.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            5244..6746
                     /locus_tag="U4B03_002518"
     CDS             5244..6746
                     /locus_tag="U4B03_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2069325.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6759..7235
                     /locus_tag="U4B03_002519"
     CDS             6759..7235
                     /locus_tag="U4B03_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2069326.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(7332..7442)
                     /locus_tag="U4B03_002520"
                     /pseudo
     CDS             complement(7332..7442)
                     /locus_tag="U4B03_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2475 a         1197 c         1362 g         2569 t
ORIGIN      
        1 gctcatcaac ttaaacttaa tatcccagaa gatttaaaaa ttattggata cgatggtacc
       61 tcttttattg agaaattttt cccacaattg acaacaataa gacaaccaat tgatgaaatt
      121 gcaagtctta ttgttgatat tcttctgaaa aaaattaaag gcgaaaagac aaataaggat
      181 tatattttac caatctcaat actttctgga ggaagtattt aacgttaaat taatttttta
      241 atgtctataa aattcgttag aaaatacaat ttgtagatat ccaattgatt tgtaaacaaa
      301 aaggagttgt caacttgtag tgacccccaa aagttagact tttttggagt ggagaaatcc
      361 actccttttt tcatgctcta aaatgatatt tttacacaac tgttacggtt gttttacgaa
      421 attggacggg actttgccag tttaatttct gttttatccg ttcattattg tagtaataga
      481 tatagctatc gatggctgat tttaattcct cgaaacagtt ataaactttc ccgtgaaaaa
      541 tctcttgttt taataagcca aaaaaattct ccataagtga attatctaag cagttccctt
      601 tccgagacat gctttgaaag attttgtact ccttcaattt gtttctataa gctttcattt
      661 gatacgccca accttgatct gagtgaaacg tacgacgata gggacaatcg ttagtggtct
      721 ggatagcttc ctccaaacct tccattaccg ctaatgcatt gggcttttca gagatacgat
      781 aggataaaat ctcactattg aacatatcca taaaaggatc tagatacaat ttcttttgac
      841 ggataatgcc tttggcatcc acttcatggt atttaaattc agttgtatcc gtcgtgattt
      901 tctgatggca gatactggtg tagaaacgac gatggatccg atttttggct actgctccga
      961 cttgtcctcg ataagaattg tagcgccgtg atttacgtgt gtatgtcgtt acttttattc
     1021 ctagcttctt gatcaggcgc tgaactttct ttttattgat agagaaaccg cggtttctta
     1081 gttcattggt catccgtaaa cagccataat ccttgtgctt ctcccgaact ttaagcattt
     1141 ccgcttcaat ctcttgatct ggatttttac ggtccaatcg tttttgccag tacataaaga
     1201 ttgccttagg gaactttaaa gtttctagaa gctcaactaa tttaaacggt cctcggaggc
     1261 ttgcgatgat tcgtgcgatt gcttcattcg tcgttgttgg gcttcctgtt ttcgcagctt
     1321 cctcaattct tttaaaaagg cattttcaat ttgaagggaa cggactcgtt tctccaactc
     1381 ctcgatgcgt tcacgttctt tagacgttgc tttagttgta gattttttct tatttttggg
     1441 ttcatttttc ttagacaaga taggtggaca tcctttcgtt tttgagagtc catcgatccc
     1501 accctcacgg aattgacgca tccaactagc gatcaaatta gggttgttca tgttaagtgt
     1561 gttggcaact tcacgatagg acagctcact tgttagatat aactctatcg catccagctt
     1621 aaattgaaca gaatattttt tcttttgtcg ctttcttact agaccctctt caccaaattc
     1681 ttgataggca ttgatccagc gcttcacttg tgatggagcc ttaatggaat atttctttgc
     1741 cagataatca cttccgcctt taccatccaa gtaatcatga acgatcttta atttaagttc
     1801 aaaagtatac ttagccataa aaaataccga cctccaaaag ttagattttt tggtctaact
     1861 tttgggggtc ggtacaaaac ttaaaagttt tagacccttt tattttctta atgacttcag
     1921 tcagttttgt gcgtaaagcg tgcgaaacaa gaaaaccgcg cgtttctacg cgtggaagaa
     1981 gtatctttag cgataaaaac tcctttcgat atactagtga gtggctacca accgctcatc
     2041 agatcgaaag gagttttttc atgtcaaatg acgataaaag tttagcatat actcgttgga
     2101 attgtaagta tcatattatt tttacaccaa aatatcaacg aaaagtaatc tatgggcaat
     2161 tacgaaaaga tcttggaaaa atacttagga agttgtgcga aatgaaagat gtggaaatca
     2221 tagaagcaca tgcaatgcta gatcatattc acatgcttgt tcgagtacca ccaaaaatat
     2281 gtatatccag ttttatgggg tatctgaaag gtcgtagtgc ggtactaatc cacgagcagc
     2341 acgcaaatct caaatataaa tacggaaaca aatccttttg gtcgaagaga tactatgtga
     2401 gtacagtcgg attgaatcaa aagacaaatg aaaaatacat ccgtgaacaa gaagctgatg
     2461 atcgcattcg atatattata agtaaaagag agtacgtaga accatttaaa aaataaagag
     2521 aagtaagtga gtgaagatga gcagttggcg gtcttttaaa aatccccttt taaggggcag
     2581 cgagtgtcga ccccttctag ggggattaaa aatacacccg tcctcacggg tgtatgggtt
     2641 actgatagac ttgattttaa ctggtgtcag aaacttatgt ccagaaaggg tgatgaaatt
     2701 ttggcaattt acggctattc aagaattagc acaagcaacc aagattacaa aacacaaatt
     2761 caaacattag aaaatgctgg cgcagaaata atcttcctag aaaatcactg gattatcgaa
     2821 cacctttgga agtatttttg agttacgtaa gtattgatga tctgtctaac ttaatttgac
     2881 aattaagagt atatttactt cagatggtaa ggcaacaaaa taaaatacaa taggcagatg
     2941 aactaataaa taaaatctgt attaaataca gataaattaa tataaaaatc aaaaggatca
     3001 taaactttta acaagtgatg aaatgggtgg aaattgattc ttatagacag tttgtctcat
     3061 aacattgaag tattttgcaa ttttagcatt gaaacacttt tttaatcttc aacgttgttc
     3121 ttataaacaa gatgattggg aatcttttga attaacactg tatagcttag aacacccttt
     3181 atagaaaaca ttcgcttagc aatcttaata attccttgtg cccgatgtcg tttagattca
     3241 gtaggttcct gctcatcgat catggaaaaa atttgaagga tcagatcctt aataaattta
     3301 tttaagagag gatgatccat tgcttcatcc atactcgata aactagtgat aatgagctac
     3361 acgtttttct ttcaagttga tcaatagctt ccacgataaa aatatcttta gataaaatca
     3421 agaaccgtag gaactacagg gatatttggt caataagagc caccgctatt agtaaaaaat
     3481 aagctatcaa gtatgctctc atcccaagaa ttgcctactt caatcgttaa aaaccgttag
     3541 gtttagctaa gtgggggtag ttcactcaat acttttatca ataatagagc gcaaaacttt
     3601 ttctcttaat tcaactgaat caatattatt gttttgttca taaccatgaa ctaatagatc
     3661 acaaatataa agttgtgata tttttccagc taaagatcca ccattaagaa attcttcaat
     3721 ggctgtttgt aaaactaagt ctgcagactt tcctataggt gaatgaatat agttagttat
     3781 agcaataatt tttgcgccat tttttttagc tattttcaat gaatcataag tatctttcgt
     3841 tttcccagat aaagaaaaaa tgattactaa atcattaact gttaataaag aagcgacttg
     3901 ggcttgaaaa tgagggtcta gtacagcttt tgcttgaact cctacgcgaa gaaacatatt
     3961 ttctaaatca agactagtat ttccactaga tcccactcca aatatatata tgttttttga
     4021 ttgagtgatc attttaatgg cttcattcaa tttttcttcg tctaaaagcc taatagtgga
     4081 atagagagct tctgttaaac tattagcaat ttcatcatag tactttacac tagtcggatg
     4141 ttcttttttt tgactaaagt cttcctttgc aatttcaatt tttaaatcag aaaaaccaga
     4201 aaaaccaatt ttttgacaaa agcgaataat agtagcatcg ccaacttttg tgtttttctt
     4261 aatatcactc atcgtactat aaataatagt tttcccagaa tttaaaataa aatcagcgac
     4321 ttttttttca gacttcgtta aaaatggata attttcttta attaaataga tataactcat
     4381 acaaactcct ctacaatttc aacttttctc cattatactc caaaataata aaaaaaactc
     4441 ctaaatactc catttttatc tttactttta tgaaagcgga tgctatattg tttttggagt
     4501 tgttatccaa aaataataaa aaattggagt gtttaggata atggataaaa agaggttttt
     4561 agatcttatt tcaaaaggtt tgattgtttc ctgtcaagca ttacctggtg aaccatttta
     4621 caaagaggaa gaaagtttaa tgccttattt tgctttggct gctcaaaggg ctggagcagt
     4681 tggtatacgt gcaaattctg ttcgagatat tattgaaatt agaaagtgtg tatcattacc
     4741 aatgattgga attattaaaa gagattatcc accagaatct ccattcatta ctgccactat
     4801 gagagaagta gatgaattag tagaaaccgg tgttgaggta atcgctcttg attgcactct
     4861 tagaaatcga catgatggac tatctataaa ttcttttatt actaaaataa aagaaaaata
     4921 tcctcatcag ttatttatgg cagatatttc tacatttgaa gaaggcaaaa atgcatataa
     4981 agcaggaata gattttgtag gaactacttt aagtggatat actgaggaaa gtaaaaaaca
     5041 agatgggcca gatatagatt tgattaaaaa attggtagaa gaagggattt gtgtaattgc
     5101 tgagggaagg attcatactc cagaacaagc aaaagagata aaaaaaatag gagttgcagg
     5161 tattgttgtg ggtggtgcta ttactcgacc acaggaaatt gcatcaagat ttgttaaagc
     5221 attaagttag ggggagaaat aaaatgttta aaaaattttc acaattagga cgcgctttca
     5281 tgttaccaat tgctatatta ccagtagccg gattactatt gggactggga ggagcgttaa
     5341 caaatgagag tgctataaat gcttatccat ttttagatca accgtggttg catacaattt
     5401 taagtattat gagttatgct ggaaatgcag tttttgcaaa cctagcacta atttttgcaa
     5461 ttggtattgc tgtaggactt gcaaatggag ataaaggaac agctggttta gctggtggag
     5521 tagcttatct agtatatact gcaactatta gtgggtttct tgaacttttt tcagcaaaag
     5581 atgcaacact agatacaggt gtagtgggat caattgttgt tggtggtact gttgcatttt
     5641 tacacaatcg ttatagaaaa attgaattac cacaattctt aggttttttt ggaggatcaa
     5701 gatttattcc gattgtttct agtttagctg caatcattat tggttctttc ttttatttaa
     5761 tttggccacc aattcaaaat ggtttagtag ttgctggtga acaaattgcc caaatgggta
     5821 gtttaggaac ttttttatat ggttttttat tgcgtttaac aggtgctgtt ggactacacc
     5881 acaccattta ccctttgttc tggtatacat ctttaggtgg taccgaaact gtagctggat
     5941 caacaattgc tggagcccaa aatattttct ttgcccaact agctgatcca aaccacactg
     6001 gtctgtttac ttatggtact agattttttg caggaagatt tgcaacaatg atgtttggat
     6061 taccagcagc atgttatgca atgtatcgtg caattcctaa gaaaaatcgt aaaaagaatg
     6121 gtgggcttta ttttagcggg gcccttacat catttttaac tggaattact gaaccagttg
     6181 aatatatgtt tttatttgtt gctccttggc tttatgtgat tcatgcattc ttagatggat
     6241 tatcttttta ttttgctgat atcttaaata ttcgtgtagg gaattccttt tcaggtggtt
     6301 tgatcgactt tttattattc ggtgtacttc aagggaatga caaaactaat tggttaaaat
     6361 taattccttt tggtattgca tgggcattta tatatttctt tgtttttaca ttctttatca
     6421 aaaaatttaa ggtagctatt cctggaatgg aagaagaatc agaagttcat tctgaaaaag
     6481 taataaacaa aaatacatca ttacatgaag agtctcttat cattattgaa gctcttggtg
     6541 gaaaagaaaa tattgagtct gttgaagctt gtgcaactcg tttgcgtgtt gctgttacga
     6601 atggggagat tattcaaaaa caaacaataa aagacactgg tgcaactgct gtcttcgaag
     6661 tgaagggtgg catccaagct gtttttggag ggaaagctga cttattaagt caagaaatca
     6721 atcaaatatt aggaaaagat aattaggagg tttttaatat gtttgaaatt ttcaaaaaga
     6781 aaaaacaaga aactttttat gcaccttgtt ccggaaaaat catttccatc acagaagtaa
     6841 atgatcctgt cttttcacaa aaattactag gtgatggttt tgccgtcata cctgatggtt
     6901 caaaaattta tgctccattg gctggtcgag taacaaatat atttcctact aaacatgcta
     6961 ttaatataaa aagcaactca ggcattgatt atttgattca tattggaatt gatacagttg
     7021 aattacaagg agagcctttt tcgatttgtg ttcaagaaaa tgatgaggtt actcctgaaa
     7081 cctcgttggt tgatgtggat tttgacaaaa taataaaatc tggtaaagat acttctgtta
     7141 tagttgtttt tccggagaaa aatcagttca atgatttaat aatagataat ggtcgaattg
     7201 ctcatgggaa ttcatgtgga aaaatcactt tttaaaaaag tgtatataaa aagctataaa
     7261 ataagctaaa taggatattt gtcaaatgaa gttggtgaac agaaagaact gttttctttc
     7321 tgttcatttt tttattttat tagtaatgat tccccatgcc aaatcatgat ccgtaattta
     7381 aagttataaa agcttcgaaa tccattggca tttcgttgga ataccttgat atgattattc
     7441 aaatataaaa tatagagaga agagagagag ggaagatatt tataaagggc aaagttatgt
     7501 ttactcaact taggaaatag aaatagatca atttatattc tcttgtcaat cgaatatgaa
     7561 ccttagcact atataccaaa tatagtaaaa tgctgaaaga tgt
//
LOCUS       DASJSZ010000110         7412 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.116, whole genome shotgun sequence.
ACCESSION   DASJSZ010000110 DASJSZ010000000
VERSION     DASJSZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.116"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="U4B03_002521"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="U4B03_002521"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEN2069327.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="U4B03_002522"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="U4B03_002522"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN2069328.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="U4B03_002523"
     CDS             complement(2298..2960)
                     /locus_tag="U4B03_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2069329.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="U4B03_002524"
     CDS             3144..4790
                     /locus_tag="U4B03_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN2069330.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="U4B03_002525"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="U4B03_002525"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2069331.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7412)
                     /locus_tag="U4B03_002526"
                     /pseudo
     CDS             complement(5971..7412)
                     /locus_tag="U4B03_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2319 a         1363 c         1371 g         2359 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacatatgga aatgatctgc ta
//
LOCUS       DASJSZ010000111         7309 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.117, whole genome shotgun sequence.
ACCESSION   DASJSZ010000111 DASJSZ010000000
VERSION     DASJSZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.117"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..642
                     /locus_tag="U4B03_002527"
     CDS             <1..642
                     /locus_tag="U4B03_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069332.1"
                     /translation="RKSTTKFANSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHH
                     YFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILD
                     NPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEG
                     EDKEKRIDNIINYINSLSEPLKCRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            825..3500
                     /locus_tag="U4B03_002528"
     CDS             825..3500
                     /locus_tag="U4B03_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEN2069333.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVSITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3682..3921
                     /locus_tag="U4B03_002529"
     CDS             3682..3921
                     /locus_tag="U4B03_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069334.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3937..5310
                     /locus_tag="U4B03_002530"
     CDS             3937..5310
                     /locus_tag="U4B03_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2069335.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5314..6948
                     /locus_tag="U4B03_002531"
     CDS             5314..6948
                     /locus_tag="U4B03_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2069336.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7111..>7309
                     /locus_tag="U4B03_002532"
     CDS             7111..>7309
                     /locus_tag="U4B03_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069337.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2608 a         1139 c         1453 g         2109 t
ORIGIN      
        1 cgaaaaagta cgactaaatt tgcaaactca ttctggtttt taaaaaatat taataaaaca
       61 gtggaaaaga atggagttta taaaggtgat tttcattttc aaaatgctga gactattgaa
      121 cgagaacatc attatttcga tttattagtg aatacagtgg tagaattagc gaaatgggca
      181 aagtcaaacg aaacttatag tcaagtactt aaaagaatta tgagtcaaga aacttcattt
      241 atctatgagt taagcaagtc aaatttgaaa tatacttata atgaagatga acttgaaatt
      301 ttagataatc cagaaaattc acaatggatt aaagagttga agagagaaga ctttattgag
      361 acactaagaa ttctaaaatc gttatttgat ttgaaattag ccaaagctac catcatggga
      421 attgaaagag attcatatta ttgggaggaa attggactat atgctcccca gattgaaggt
      481 gaagataaag agaaaaggat agacaacatt attaactata ttaatagttt aagtgaacct
      541 ttaaaatgta gaggacttga ttttatagga acattaggat ggacagaaac atactatgag
      601 aaaatatata aagaaataat tgaacaatat tttcctaaat aatttaataa ggattgattt
      661 atcgtaaaag aaaaaagttg tgatttccaa aatcacaact tttttctttt gtcaagattt
      721 tgagtgggat atatttttgt agaactgaag atcatgaaat tatgagaggt tggaggtaat
      781 gtagtcatac aaataaaaat tttttagaaa gtgagtggta attaatgaaa atgaatcaca
      841 cttcaattca gaagcattta caactgttaa atacactggg gatttcttat gttaatttga
      901 gaactttttc tacattaaac aagaaaaccg tttctcatat tttttctact gcagattctc
      961 aagcaattat taactattta gaaaaacaga aattgtctga aggtacagta agcataactt
     1021 ataatcctat taaacaagag gtgattacac aaaaaaagca ttctgtgaga gacaatgata
     1081 ttgaaaaaat tcggtttgta tttattgata ttgaccctaa aaggaaagag ggaagtgcaa
     1141 cagatagcga aaagaaaaag gcagaaaatg ttatggaaca agtagaacgt tatttgaaag
     1201 aaaagagaat agaatcattt gtaaaagttg atagtgggaa tggctaccat atttttcttc
     1261 ccataaatga acaaccaaat aatcacgaga cgattcttac tattcagaat tttcttcaat
     1321 tacttcaccg aagatttgga gtagaagatg aagttgatat tgatacttct gtttataatc
     1381 cttcaagatt atgcaaatta gttggtacac cagcggtcaa aggaaagtcc acaaaagagc
     1441 gtccgcatcg tttatctagt ttcctttcta ttcccgataa catagataga agtaattgtt
     1501 ttaaaattct tgaacagata gttgaagaaa attccatgat gtcaacctct agagtaaaaa
     1561 gccatccatc ttttagagaa gtgttacctt ttattcaagc agactataaa caatggttag
     1621 ctcactatcc tgaattaact tataccataa aagagggcga tcataaagga gttaccttac
     1681 ttatatttga cgaatgtcca ttaagaaaac acacgaataa ttctaatggt tcttgtttgt
     1741 ctgttacggc tgataataag attcgatttc attgtctgca tgcttcggat aaaaataaag
     1801 atattcaaga ctttgtaaaa aaatatccgt tgccaaaacc tgtaaatcct tctgtagaat
     1861 cattagaatc caatcaagaa tattattttg accagtttaa actgaatcaa gaaggagtat
     1921 ttttggtgaa agaaggtcaa gaagaaaaag ttgcttgtcc gatgtttatt aagcaaatac
     1981 gcaaaaatat cgaacaaaaa gtcattacac ttgttcttgc ctattatgtc gatcattgtt
     2041 gggatgaaat agaaatagaa ggtggatgtt tacaaactaa taattttaaa caattagcaa
     2101 aacatggctt aatcattaaa gctagaagag aagctgaagt cgttgacttt ttacagattc
     2161 aacgggagaa tgctccaatc ttttttgaac atgaatctct tggctggaaa aaagaaaatg
     2221 aagcaatgat ttttcagtta caagattctt ttggacgtga aattccttct gtattaacta
     2281 aaaactcatg ctaccaatta acaactaatg gtacagaagc ggaatttgac gcattggttc
     2341 ataagtatgt tttaggaacg aacttagaac ttgcttggtc gattggtatt gcttctattg
     2401 tgttaggcta tttaaacgtt acgaaggcta atactttttt gagtttacta attaacctac
     2461 agggcaagag cacaacgggt aaaaccacta cgttacactt tattgcctct ttatttggaa
     2521 atccaactac ggttcttaga aatatgaatg ctaccaataa agccattata aagctagcat
     2581 caaacaatca gggaggtgtt ccactaatac tagatgagtt aggagcagca actcaaaata
     2641 actttagctc gttgatctat caattagctt caggagaaga aaggctaaga ttagattcta
     2701 attgtcagct tatgccacaa gagacttttt ctgttttgac ccttacctct tctgaagagc
     2761 gtttggaaaa ttatttcaat gagaaattaa ctggtttgaa agttcgatat atcgaatttt
     2821 ctgatatcaa gtttacaaag gatgcgaatc acgcagagaa agttaaaagt ctttcttcta
     2881 atacttttgg ttggggtata aagttatttc ttttgaaact ctttgaacaa gggattacaa
     2941 aaattgaagc aacttttgtc gaagctcaaa aaaaactatg ggctgtttta cctgaacatt
     3001 ctttgaagca acgcattggt aataatctag ctttgattta tagtggagcc ctcctaattc
     3061 aagatatact gggctatcct ttacgattag agcatataca gacggtgctt attaattcgt
     3121 acgaacagtt aattgtagat actagtttag gaagtgagca aacggtattt gaccgagtga
     3181 aaacattgtt ggtgatgaat agccataaat ttttaacaaa cagtgacaac aaacatacgc
     3241 ccagtacgat ttggggaaaa gtatcaattc aaaaaaatgg ggatattaag gtaaatattt
     3301 ttcctatccc gtttgaggac ttgttaaggc gagaatttca agtaaaggat ctaacctatc
     3361 tttttaggca attgcttgat tcgggtaata tgtgtgcaga aaaagggaga agaacgaaac
     3421 gtattcacat gaatcgtcaa gttcttccaa catacgagat tctattacct tcttcactca
     3481 aatcgtattt tcgattgtaa tttagaataa accaaagtat gaaagtgagg caccatgtta
     3541 tggaaatatt tcttattgat ttagcaaaac aactgattac ttatctgtat gatagcgagc
     3601 aaacccaaac ggaagtaaat gaagcttgat ctatgcttca tttacttcat tcaatcaaac
     3661 atgaaagaaa ggaaaattct aatgactaaa aataaaatga cactaaaagc agaggttctt
     3721 ctgtatattc aagaacattt ttcaaatcaa gcctttttta caaaaccaat ttacttggat
     3781 ttcgaaataa gaggggtatc tgctggatct atcgggggta ctcttcaagc gttgaagaac
     3841 gaaggatatt tagaaaatca ctttgtgcaa cgttcgttca atagaagagt cgtgaaaaaa
     3901 tggtatttag ttcattcata gagaggggaa tcaagcatgc gtgtggcttg ttatgatcga
     3961 acgtctgttt atgaagaagc gaaaattagt aatgacgctc aatatgatgc agtgaaagag
     4021 tatgttttag agaaatatga acaaattccc caacgatttt cagacaaagg agaatcaggg
     4081 gaaaaagcgg agcgtccagc ttttcgacag cttagagctt gggtttgtgc gtcagaagaa
     4141 gagggggtga tctatgtttg gcgttatgat cgattggcac gtcaagccaa aatagcctta
     4201 gaatttattg agttgtgcca acaacatcag gttcaagtgt attcgatttc tgaacctctt
     4261 cctgcttctt tctcacacgc gtcaacgaat aaactatttg tgcaattatt gttcactttt
     4321 gcagaacaac aacgaaacgt cattattgag aatgttcgtg ccggactttc ctataaaaag
     4381 caacaacggc aatatttgtc ttctgcggta ccatatgggt atcgtttagt gaatgggaaa
     4441 atccaacaag aagtacacga agcaaggatt gttcgctata tttttcaact ttatttgacc
     4501 aaaaaatatg gctacaaaaa gctttgtcaa cgattgacgc aacaaaagct tttcttccgc
     4561 gaacgtcctt ttcagcccta tcatatttat agtatcctaa aaaatccatt gtactatggc
     4621 gaaatcaaag gaggatcttt ggggaagtat ctaggaacct ttgaaccgat tctctcgaaa
     4681 gcaacctttt tacaagccca agagattcgc caatcaaggt gtacagctaa aaaagatacg
     4741 tatctgtact tattacgtca aaaaattcgg tgtcccttct gtggtcgtca tctgagttcg
     4801 aagtatcaat ggaatacaaa gaagacgaag acgcttcatt attatcattg taccgaccga
     4861 agctgccaag gaatttttct tcgggcagaa agggttgagt cagaagtatt ggaaaagata
     4921 caggcttttt ttcaatacga aggcgtctat caagccttga ttgcccaatt aacgcaacaa
     4981 ttagccgaaa agcgaaaaga tgaaaaacgc aaagtaatcc aacaaaagaa acaagaagca
     5041 caatggatta aggcatttga agagggcaat tgttcactgg aagaactcaa gcaacaactg
     5101 caagctcttc aaaagccgat gtctacttct tcgtttcttt cactccaaca gtgtgaagca
     5161 aaagtgaaag aatggcttca actaaaagac ctctctagtc gacaattatt atgtgactat
     5221 gtttgtcgga ttgaggtaac aaaggataaa acagtttgtg ggatttactt ccgacaactg
     5281 acacaaaata ttttagaaag gaacgaatga tctatggaaa tgatacaaac caaacgagtc
     5341 gcattgtata ttcgcgtgag tacacaagaa caagctttag aaggacatag tattcaagca
     5401 caagaacaaa atggacgaca gtttgccgaa cggatgggct atgaagtggt agaagtttat
     5461 gccgatgaag gaatcagtgg gaaatccaca aaaaatcggt tcgcctatca acgaatgatg
     5521 aacgatgctc ggaaccatcg ttttgatcta ctagtgattt ggaaattgac ccgtctcgga
     5581 cgcaatatgt tagatgtctt acaaacagtc gaggaattat tgcgatatga cgtaggatta
     5641 cattcgatct cggaacaatt tgacgtgacc acttcgacag gtaaattgat gttacaactc
     5701 cttgggagtt tcggtgaatt tgaacgcaac caaattgcgg agaatgttca aatgtcaatg
     5761 caaagcttag tgaaagataa aaagcgttat gcaggaggcc gacgtttagg ctatgtcagt
     5821 ggtattgaca cgaatggtca aaaacaattg gtcattgaac cagaagaggc gaaaattgtt
     5881 cgtctgatct atgcaaaata tttaggcgga gcagggtatc gagcgattgc caattttttg
     5941 aatcgtcaag gatatcaaac agtcaaaaag aatgcttttt ctaccacggc agtcaaagat
     6001 atcttacgca ataaaattta tgcagggtat ttagaatatg cgcgctacat caattgggaa
     6061 accaaacgac ggaaggggaa gaatccgaga ccgattttag tgaaaggaac ccatgaaccg
     6121 attatttcgg aagaagatta tcaaaaagta caagaacgcc ttgctttaga aggaaaacaa
     6181 cccaaatgga accatacagg tgaaaatgta ttgacaggct tgttacgttg tccagaatgt
     6241 ggcgcaccga tggcagcgag taatgtcaca aataccctaa aggatggcac aaagaaacgg
     6301 attcgatatt attcttgttc agtctttcgg aataaaggag caagtgtgtg tcatgccaat
     6361 tcgattcgtg ccgaacaagc ggaaacattt gtcgccgacc gattaaagga gatcgttcag
     6421 ctaccagaag ttcttccccg attggtggca gcgttaaatg aagaaatagt gcgacaaagc
     6481 caaccattgg aacaagaact cgtggtgtta ctggaacgaa aggaagaatt gaagactaaa
     6541 attgaaaaat gggaagctgc tttggaagat agtcctgaat tgtttccgat gttaaaagac
     6601 cgcttagacg aattgactga aaaacgacga cagttgcaca ttcgagaaaa tgaaatctta
     6661 gggatctttc aacaaggaga gccgattcaa gtcaaagatg tccaacggat attgactagt
     6721 ttggatcgtt ttcttgccca gagtgagaag aagcaaatca aagccttgta tcgtaccttt
     6781 atagagaaaa taacctttga tcctaaccat aaggagatgt tggaaattac gatgaaattc
     6841 acgcatgccg tggtacaaca attgaatgag caatatcaaa tagccgtatc caaaacaaag
     6901 gatacggcta tttttgtgtt aaaaacaccc tttacgttaa cgatctagtc taggttatat
     6961 caaacaacgt tagaggggcg ctaacgcttc tctagcgatt tgaaatcgtt agagaagtag
     7021 ttagggaggt ccttatcata aatagggata tttattaatg aatatttgaa atataattgt
     7081 atataagaat taaaaagagt ggggatagat atgaaaaaga tgggtaagtg tattttatgt
     7141 gaaaaataca ctgaacttac taaagaacat gtaccaccta aaaaatcagg gaatactgga
     7201 ggtaaaaaga ccagaactgg taatttagat gatttcctta agagcgattt tacaaaaggt
     7261 gattttccga aggggattaa aaggaaacct caaggaaatg tttattata
//
LOCUS       DASJSZ010000112         7182 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.118, whole genome shotgun sequence.
ACCESSION   DASJSZ010000112 DASJSZ010000000
VERSION     DASJSZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.118"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..334)
                     /locus_tag="U4B03_002533"
     CDS             complement(<1..334)
                     /locus_tag="U4B03_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2069338.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTF"
     gene            536..835
                     /locus_tag="U4B03_002534"
     CDS             536..835
                     /locus_tag="U4B03_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069339.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            1100..1975
                     /locus_tag="U4B03_002535"
     CDS             1100..1975
                     /locus_tag="U4B03_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069340.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1972..2190
                     /locus_tag="U4B03_002536"
     CDS             1972..2190
                     /locus_tag="U4B03_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069341.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            2177..4804
                     /locus_tag="U4B03_002537"
     CDS             2177..4804
                     /locus_tag="U4B03_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069342.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4819..5037
                     /locus_tag="U4B03_002538"
     CDS             4819..5037
                     /locus_tag="U4B03_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069343.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            6556..6870
                     /locus_tag="U4B03_002539"
     CDS             6556..6870
                     /locus_tag="U4B03_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069344.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
BASE COUNT         2786 a          902 c         1347 g         2147 t
ORIGIN      
        1 aaaacgtatc actatatctt tcttgaatgt ctttaaagac tttgtgaggt atttctggga
       61 agtccttaat atatagctca taccctatat cgataaggta tgaagttata tattcttcta
      121 aagttcccca tttaatccag tcaattgttg aattagtgat agttgataat aatttcagct
      181 tttgtggatt tggtaaattg gtcccacgtt cccagttgga tatggtcgca cgtggtgaat
      241 tatctatttt ccttccaagt tcttccatag taaaaccaca tgaaaggcgt aattgtttga
      301 ttcgttttcc tgcttctgaa ggatttggct tcatcttttt tctccttttt atttttaaag
      361 atagtatatc atatttataa aataaaagta tttaaaagta tttacttttt atctggagta
      421 tgatacttta ttcttataaa tacaaataaa tacatttttt tacgcaattt gtatttattt
      481 gtacttataa aggtagagga gttatagaat aattaattaa aaaggagagg tttttgtgga
      541 agcaataaaa ttaatgattt cagaagaaca ggaggaaatg tttctaaagc agttttatca
      601 aattgctaca aaagctattg aaaaggctca agaaaatgcg gggatgacaa gggaatatct
      661 caatcaaaaa gaaatggcgg aatacttaaa tgtgagtgaa aactttttaa aaaatgctgt
      721 gagaaatgag ggattgcccg tagttgaatt aggggcaaga aaattttatt cgaaaaaggc
      781 agttaatgag tttatgtttg ctcgaatgaa aagaggggaa aatattggaa gatgatgaag
      841 tagaaattat aaaaaaatgc tttaaaaaaa gaaaaaaagt gttcccaaaa gtttgcagac
      901 tgggggaaca ctcaataata aagtgctgag aaaactttat ttatagctat tatatcaaaa
      961 aagaaggtga aaaggtgttt tcttctggaa atgttgtagt tgacaaactt agagaaataa
     1021 attttgacgg aaatgtaatc cacacacatg gtacgatcaa ttaagaaaaa aaacgaaaaa
     1081 aggtgttgag aagccctatt tgaatgcaat agttatctta gcagaaatta cctattggta
     1141 tagacctaaa aaaattttta acgatgatgg tcagctactt ggttatcaaa aaaagtttct
     1201 tgaagatatt ttgcaaaaat catataagca attaagcaaa aaaacaggat tatcagaacg
     1261 agctataaaa gatgcaattg tgtacttaga gaaaaagggg attatcaaaa gggtttttag
     1321 aaatatttat tatgagaaaa gagcagttac attatcaaat gttatgtaca ttgttttaaa
     1381 tgtacataaa ttgatagaaa ttacttttcc tactttggat tgcgaaaaaa aggagtatat
     1441 gccgaaaatt cgtcagaggg gtatcgaaaa aaagagatat gtctctttaa aaaaaggtag
     1501 aggtatgtac aaatttgtat atacgtatac aaagactact tcacagaaca tatcaaagac
     1561 tactctcgtt atggaagaag aggatattaa tataaagagg atttacagta agattagtgt
     1621 tctttatgac gcaaataacg aagatggtcg aaaattattt caagtgtatc actatttgag
     1681 aaacgctaat gtagatacag aagatatcag aaaaatcatt gaatatttat ctgaaaatac
     1741 tcagtatctt attgctgagt gtattataaa acaacatgaa gcatgctttt ttcaaagaaa
     1801 aactgaagaa gggttatacg attatgtaac atattttttg aatggacttc agaaaagata
     1861 taaaagtcaa tttttagaaa atgtagagcc tatagatcag ttcaaaaaag ctttcgaaat
     1921 caaagagtta cctgaaatta ctatgcataa ttggttgaaa ggagaagaat catgattaaa
     1981 aatcgcttaa tagatcaaat gtggtgtctt gatcaaaata cagatactct cgaattaatc
     2041 gagagtatct gcttcaacac agtagttctc gatttaagag ctgaaaatga caattgtgta
     2101 actcatatca taattaatca aaaaatggct cagcagctct ctgagagcct aagaaaatgg
     2161 gctgaaggag aaaactatga aagcaattaa agaaaaaatc gttagtagaa aaataaactg
     2221 gttagcactc agtgtacttg ttttaatgat tagcgttgtt tttacaagta atattgttga
     2281 agctagcatt ttcaattggt ttgatgacac aaaaagtcaa acagagtttt tgaataattc
     2341 tctttataga ccttacctaa aaaaatacaa tggagatttc cttagtcagt tcgggatttg
     2401 gcttggatgg gctgtggtaa aaggcatgtt tactgtgact gattcgattc aaaatatgat
     2461 tccagatgtg ttggatttgt tcaatttcat tgaaagtaca gggctaaata atgtttatca
     2521 atcagtcata aatacaattg tcgttggatt aatgatcctt tcattgatgt ttgttggcta
     2581 caagatgatt actggaaaag gaacaataga tttaagatct gttggaatga atatagtgat
     2641 gtctgtagcg ttaattcttt taatgccaac aatgatttct agcggtattc aatttgccaa
     2701 aatattttac aatgatgcta ctacgattac aaattcagat gacggagtcg cttggtcatt
     2761 gataaaacaa ggagtaacag atctggcata tattaataaa acagatcaat actcaagcat
     2821 tgataaaacg gaggatcgaa ataagttaac acgaaaaaat tttcaacaga cagatttaac
     2881 acaagtgttg acggataaag tgattgataa attggaaaaa gaaaatccag ctgcggataa
     2941 tcttcgctat gaattagttg aaaattctaa taatgaattt gttgccacaa agttttctga
     3001 taacttctta tcaacatttt ctgatagctt gaaatcaggt tattatcgat atcaagcaaa
     3061 tctttggggg atttcgattg ggttgacagc gttggcaatc gcctatgtgt tcagcgcgtt
     3121 tgtgatcatt acagcaatat tagaacttgc atttaaacgt gtgttaggtg ttcttgtttt
     3181 tgcgacagat attgaaacag gacaacgttc aaaagttgtt ttatccgata ttttacaatg
     3241 ttacttaaca gttggatttc aagggtttgg actatctatg tttgctatgt ttatcaattt
     3301 tttaaatgct ggacaaggta tctccacaaa tatttttata aaaacaattg cttatatctg
     3361 tgctgtgttt gtgctgataa aaggcagtgg aacagtcatg agatactttg gtgttgatat
     3421 tggactcaaa gaaggctatg ggcaacttgc ttctgctttt ggtatgggcg caatgctgtt
     3481 tagaaaagga tcaactggat ttaatcgtgc taaaggaagc ggaaatggaa atggatcaga
     3541 cagtggagaa ggagaagatc gaaaaccaga aaagaatttc ggcgaaactc tatcaaaaaa
     3601 agctggcaaa actggaagag ctttgggata tgcccatgaa cgtggattat caggtttagc
     3661 ttcagatgga gctacaatgg ctagtgaaag agcaataaaa ccatttaaga gtatgcgtga
     3721 tatggctaat gataccaaga ataagttcaa agaaggttta gatgatggta cagtgtccgc
     3781 tattaataaa aatagcaaac ctatgttagc taaaaataaa gaagatgaaa ctggaaaata
     3841 tgctgattcc atgccaacac gtctttcgga tagaaaagat ggtgctaagg ttgaaaatgc
     3901 cgatagaatt atgtctagtt ctgaacgtat gcgtgaagca atgaaaaata atgctgaatc
     3961 gaataataat cctgtaagtg caattcagca aaaagttcaa caagatattg aagaacgtaa
     4021 aaatgctatg cacggtcaag ctaagtctgc tgaagaatta attaatcaga agcgacaaga
     4081 agctaaatac acgcctgaag caatgaatcg tgaggaaatg ttaaggaaac gtgtagaagg
     4141 gcgtacagga gctaatacgg acgaaaaaga agcgttgacc aaagaaagaa tccaagaagc
     4201 taaaaactca aataccggat tagaaaaatt ggtcaaagaa aatctacaaa attctacaag
     4261 tagtcaaggt ggccgatcag tagatgtccg agaaaatatt caaggttcat tgaatggtca
     4321 tgtcggacgg aatgttgatg taagagagaa tttacaaagc tcttcaaatg gtcaaggtac
     4381 taagacaatt gatgttcgag aaaatgtaca aaattctaca agtagtcaag ggtttaaatc
     4441 aatggatgta agagagaatg tacaaaattc tacaagtagt caagggttta aatcaatgga
     4501 tgtaagagag aatgtacaaa gttctacaag tagtcaagga ttcaaaacag tagatgttcg
     4561 ggagaacgtt caaaaagata gtggagaaat gaaagaaaaa acacaaaaaa tcaatatcgt
     4621 tgaagacaga aagtcagcga aaaaatttga ttcaaatcat gaaacagtga ttattgatag
     4681 tgaaatccgt gaaaatgata agggatcaaa accaagacga agatttacct atgaagacaa
     4741 cgagttattc agagatacac ttaatgatcc taatccgctt ttcgataatc tattaaaaaa
     4801 ataaaagggg gaattgtcat gaaattaatt ctaaagatta gtacgcatga tttcttagaa
     4861 cttgacgaat tatgtgaatc agtaaagaac gcaacaaatg aaaaaatcac ggtcactaat
     4921 tatattgaac tgatgattca agaagaatta aagatgaaac atgagataaa aaacaacttg
     4981 aaatattgtt atctagaaaa aagtgaagag ataatccatg aattaaatgg cttttaaaaa
     5041 ttgtatttga catgaattta cgacaaggaa ttagtgggga ttaagcgatt attctcttcc
     5101 actttagaga aataatgggt taaaaagcca gaatacgtag agcaatattg acaacctata
     5161 aaggagctat tgttgaaaat tgaagtaaca aaaagataaa gggaggttta catctatcag
     5221 attaaaagtg atagtacacc catgaaacct gcaaaagagt tgaacctaat gtatatcggt
     5281 ttaactcttt ttatttgcaa attaaaaaaa tatcaccgaa gtgacgaatg aaactatgac
     5341 caaattaagc aagaaaatat atcttgcaaa taaagtagta taaaaagata ttgataaagc
     5401 caatcaaaac tttgaatttc aaacgttaga taatcaaaca gatacaaaaa atgaaatcag
     5461 ttctacgcgt acgattatta aaaataataa attttcacta gaagaaaatg gaaataaaat
     5521 caaataatta ggaagaaaaa acaaagcaat tagaaaagcc tgcaagcaaa tttgagtaat
     5581 cgagatccaa tttttttaag gaagtttaac aaaaatatgt aattctatta tcactattaa
     5641 agagaaagtt ggagcggaat ataaagaaaa gagaaaagta attaaacgca aaaaaatgta
     5701 gaacttaaaa aatataaaga ggaacaaccg ttaaaattag gaatgatgtt gtgtaaacat
     5761 tataattgct atgatataaa tgaagtgtta aagaaagcaa atttaaacca attttgtgaa
     5821 cagaaaagcc ttagtcacta tgaccaaaac tacggacgaa actatgacca aaagtaggta
     5881 ctaaattatg accgaaaata ggtccaaaat ggattttttt atgatatcaa aaattatgaa
     5941 atgattttga aagaaaacca gttggcacag ccaacttagc caccttcctg taggaaggtg
     6001 ctatcagttt tcccttatgc tcttttcttt cttttttgtt tcatatgtca aatgccattt
     6061 tgaaatgtac catcttaaga aatttatcct taaaaacaat tgcgttattg gataaaaaga
     6121 tgctacatta tcttaaaaaa ggaatcaaat atgatgaatt tagtggctag catatcgtgt
     6181 gattaagata ctttatagtg taagttgaag gagaaaaaat atttaaaata tacttcccat
     6241 ttcagtggga agtaaaatta atcttgttgt ttctactagt aatgtatctt tttggaaact
     6301 atgtaacgat gtaaacaaga tttttttcag aagaaaaaac gataaaagtt gtgataaacg
     6361 atttaaaaaa taggagaaaa tatatcaagc gcatggtatg aaacgcacat ttaaaaagat
     6421 tcgactaact aaaaaaataa gtttacaaag cattgttgtg tattacaagt gatctataca
     6481 ctgaaaaatg atagagaaaa tctgatacgt ccgcaacaat ggacaattga aaatgaaagt
     6541 gaggaaattg gaagaatggc tgatgaaaat aaagttgttc cgcttgaaac actagtgata
     6601 aataaggaag agtttgagca actttttctt gatttttttg ttcaatatga caggatttat
     6661 gattttagtt cgtcactaga tttatttgaa ttagaggcag aaataataga aaacagtcga
     6721 gaggagaatg gtaaaatcat catttcagta gaaaaattca atgaattatt ctcagaaaca
     6781 tttgaaatag atacagatta ttatcaacca caagaagttt tccaatcatt tagaaatcag
     6841 attattcgct caggtaagac cgtaaactga aaaacaataa aagaaacgtt gaaagaataa
     6901 ttattccttc aacgtttctt ttttagaatg ttttaatgac caaagcagtt tcattgtcag
     6961 gcttaggtaa ttcatgaaat cccgtattta tagtgctcaa ttgatcaact aaccatgttt
     7021 tactttttga attgtagata aatctaaaat taatatcgtt attatcgtgt tgaaggggcg
     7081 tctccaaata atctgctgta tattcatagt caacgtaacc tgctaataca aaagagagta
     7141 tccctttatt taatgttaga tttaatggat aatcatacga ct
//
LOCUS       DASJSZ010000113         7141 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.119, whole genome shotgun sequence.
ACCESSION   DASJSZ010000113 DASJSZ010000000
VERSION     DASJSZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.119"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..615
                     /locus_tag="U4B03_002540"
     CDS             <1..615
                     /locus_tag="U4B03_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN2069345.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="U4B03_002541"
     CDS             612..1385
                     /locus_tag="U4B03_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN2069346.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="U4B03_002542"
     CDS             1382..1780
                     /locus_tag="U4B03_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEN2069347.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="U4B03_002543"
     CDS             1965..2636
                     /locus_tag="U4B03_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069348.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="U4B03_002544"
     CDS             2695..3135
                     /locus_tag="U4B03_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069349.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="U4B03_002545"
     CDS             3147..3815
                     /locus_tag="U4B03_002545"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN2069350.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="U4B03_002546"
     CDS             3879..4502
                     /locus_tag="U4B03_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN2069351.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="U4B03_002547"
     CDS             4558..4944
                     /locus_tag="U4B03_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEN2069352.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="U4B03_002548"
     CDS             5157..6548
                     /locus_tag="U4B03_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEN2069353.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2277 a         1160 c         1572 g         2132 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga
     6841 gcaatttcaa aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt
     6901 gtcaggagaa caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt
     6961 atttattcca tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa
     7021 caaccaactt atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc
     7081 tttcgtagat tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga
     7141 t
//
LOCUS       DASJSZ010000114         7101 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.120, whole genome shotgun sequence.
ACCESSION   DASJSZ010000114 DASJSZ010000000
VERSION     DASJSZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.120"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(444..857)
                     /locus_tag="U4B03_002549"
     CDS             complement(444..857)
                     /locus_tag="U4B03_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEN2069354.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1313..1798)
                     /locus_tag="U4B03_002550"
     CDS             complement(1313..1798)
                     /locus_tag="U4B03_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069355.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1937..2152)
                     /locus_tag="U4B03_002551"
     CDS             complement(1937..2152)
                     /locus_tag="U4B03_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEN2069356.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2376..3176
                     /locus_tag="U4B03_002552"
     CDS             2376..3176
                     /locus_tag="U4B03_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2069357.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3195..5063
                     /locus_tag="U4B03_002553"
     CDS             3195..5063
                     /locus_tag="U4B03_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN2069358.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5056..5547
                     /locus_tag="U4B03_002554"
     CDS             5056..5547
                     /locus_tag="U4B03_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEN2069359.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5535..6089
                     /gene="srlA"
                     /locus_tag="U4B03_002555"
     CDS             5535..6089
                     /gene="srlA"
                     /locus_tag="U4B03_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEN2069360.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6107..>7101
                     /locus_tag="U4B03_002556"
     CDS             6107..>7101
                     /locus_tag="U4B03_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEN2069361.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2496 a         1136 c         1372 g         2097 t
ORIGIN      
        1 agatctgcac caaataaaaa acttccagcc aaaactgact ggaagtttat ttttttctga
       61 aatattctaa cacaaatatg aaaaagatgg ccgaagctaa taagccgatt tcgtagcaaa
      121 acaatgagat accagagtcg taataataaa atcctctaat cttatggata aaatagatca
      181 tcggacaagc agataagagt aagtatttgt taggcctttt gaataagtaa aagaaagcac
      241 tggtcattat gataaatggt atccaaaaca aaagtttact taagacaatc aaatgatcaa
      301 ctcccatatt ttttaccaag agagatcata acatctttat cgtaaaaaca aaatattttt
      361 ttgctcattt tgaaaattta aacatactcg ttcttatcca aatatataaa aatcgcccct
      421 cattcatcga gaggcgataa gttctaattt aactcaattt tttttgtttc ttttggctga
      481 taagctgttt taggtaatac caatttcagc acaccgtttt taaaggttcc agtgattttt
      541 tcttcatcaa cattaggaag atagaatgaa cgtttgtaag agaccgaatg tctttctttc
      601 cgtacatagt tgccatcttc tttatcttct gtatgggact catgattggc ggagatcgtc
      661 aaagtattat ctgaatattc aactgtcgtg tcttctttgt cacagccagg catatcagca
      721 gttagtttgt attctttttc attttcgact aaatctactt ttggataact tgaaacacct
      781 aaaaaatcat tgaatgctgg actaaacacg tcattgaaat cagggaacat gtcgcgaatg
      841 ctaggtacat ttgccatttt tgatcatcct ttcaataagt attttttatt tatccctatt
      901 tacgtaataa ctctattttg aaaaaaatgc aagtataatg gttagctttg tgaatatttc
      961 atctaatacg atacaaaggc tatctttcta atttgaaaaa gggatcatta aaataataag
     1021 aagaaaatat gtcaaaaggt aaaaaagtaa attcttctat ccaaatatag aaaaattgaa
     1081 tctagaaaac cgcccgacaa taaatttcat taactcagtt gaccgaaaat gaagtagata
     1141 ccaaaaattt atcaagagag caagtagaga agtgagtata ttcataagag aaatagctgt
     1201 aacgaattat taggttaatg caaatgctta tttatactcc gtataacttt ctcagaaaaa
     1261 atgataccaa taatgacttg ctagtactgt aaatccgtat aatatcatct aatcaaaatg
     1321 gtgttccatt tgcatctgtc atcgtaatcg atccttcggg tgacactcga taaaaaccaa
     1381 cgcttccaga tcctccttgg gaacggattt gtttgctgga agctgtgaac aaataatctc
     1441 gatcaatttt ttgataaaag ctaataacaa tatcgtcact ttgtaattcg gaacggtggg
     1501 caataagaaa tagaagtgct ttgtcagagt tttctaatgg aagagaaggt acagtgcttt
     1561 cttgcatttc agatgttgaa gaaatgctaa acgactgaat acttgattgg ctatgttgcg
     1621 aatctacatt accagtagaa gtattagtaa tggaagtagt agaagtactt ttagttgaac
     1681 caacctcagt tgttgaaatg gctgctgaag attctttaac agaattcttt gttttatatc
     1741 cacaggcaga aaaaagtatt acagttccca acaagcctgc gtaaatccct ttatgcatga
     1801 tagctcctcc taaaatagat tatctttatt cagtatatca cttaaacaaa gaaaagcaga
     1861 tggtttaaaa tgaaaaaacc ttcccaataa agggaaggtt catgaagata ataacttttt
     1921 taataattat agctttctaa tatctttcat caacttttct agtaatcatt gggaaaaatt
     1981 tcaagcagta gatcgcacaa atacctacga cgatataaat gatcttcgca aaaatagttg
     2041 ttgaaccacc agcaatcata gctactaaat caagttcgaa caagcctacc aacagccagt
     2101 tcaaccctcc cacaatcaat aaagttaatg caatagcatc taaagttttc attagaattc
     2161 tcctttctta aatgataaaa caattatata tcaaaaagag aaacattgct tatataacgc
     2221 tcaggaattt tagcaatcta tgcaaaaaga tgaaaatttt ttttgctctt ccattagtac
     2281 acatttttta gctgaagctg taaatttcat gtcttaattt tttttagtga gcttttatac
     2341 tggatttgta agaaaaaaga aggagaggat cacatatgtc ggattggttg gatattgcag
     2401 gaaaagtagt aattgtaact ggaggttcct caggaattgg aagaagtatc gtcgaaaatt
     2461 tgttgaagca gaatgcacaa gtagcgaatt ttgatgtaac tgaatgccgt atacagcatg
     2521 aaaacctact atctttaaaa gtcgatgtgt cttctaaaac ggacatagaa gaagggatat
     2581 ataaagtaat gaaacatttc aagacgatcg atggtctagt caataatgca ggaataaata
     2641 ttccttctct tctaatagat agaaatcatc caaaaagcaa gtatgaatta tcagaacaag
     2701 tttttgataa gatgatcgct gtcaatcaaa aaagtgtata tttgatgagc caagcagttg
     2761 gaagaatctt ggtgcaaaag ggatcaggag tgatcgtgaa tctttcttct gaatcaggat
     2821 tggaaggatc agaaggacag agctgttatg ctgctacaaa agcagcgatg aacagtttta
     2881 ctcgttcttg ggcaaaagag ttaggaaaga ggaatgttcg tgtggtaggt gttgcaccag
     2941 ggatactgga agaaaccggt ttgagaacac aagaatatga agaagctctt tcttatacga
     3001 gaggtataag cgtcgaacag ctgagaaacg gttacagtcg aacaagtacg atccctctag
     3061 gaaggagtgg taagctgcaa gaagtggcgg atctagtctg ctattatctt tcagaaagat
     3121 caagttatat cactggagtc acgactaata tttcaggagg taaaacaaga ggataaagga
     3181 taggggggac gcacgtgacg ctagtaaaca gatggtacca attattgaaa ttactagtca
     3241 ctcacaagaa agtttcaatc caggaagtgc aaaaagaaat caaaaccagt aatcaaacca
     3301 taaaaaaaga tattggggag ttgaatgaag aaattgccgg tattgcaaaa atcatccaaa
     3361 aaaacaaaca ttttgaactt gaaatttatg attttgattt ttttgatgag atcatgcaag
     3421 gaaaattgaa aacagccagt gatttcaatt cggcatccaa aagaatcgct tatttactaa
     3481 ataggctgat cgaaacagag gatttcttac gtatggatga tttgtctgaa gaagtcggtg
     3541 tcagcagagg aacagtggtc aaggatctaa agacattaaa agagatgatc aatgattttg
     3601 atgtaaagat tacaggcaca ccaaataaag gactaagaat aataggagat gagctagaat
     3661 tacgactact tttattttat tttgtttctc cctacttttc agagacgaaa acagaacagt
     3721 ttctagaaga aaaacaagca atcaaacatt ttcagaagaa gacgaatgct agtaaacaag
     3781 aaatcaccct tttaacgaaa gttatcggta tttctttgaa tagaatctct agtaactatc
     3841 tgttgaagaa gcccatcaat cgttacagtg ggtgttttga atatatccaa gaattcaatg
     3901 aactgatcaa tcagttggaa gaaatctatg aactaacttt gagccaatat gaaaaagatt
     3961 ttatcagttt tcctttgaac atcatcaaca caagtataac gatgataagg aaagctaact
     4021 ctgatttgcg cttatatttc gaccccatga tgatgcgtat tcgacgatta gatctcattg
     4081 atctggatga gtccttctta tatcaagaga tgaaacagca cctctcttta ttgataaatc
     4141 gggtaatttt tcgttatcga ttgactgatt tattttatgg tgagatagaa aaaaagtatc
     4201 ctttttctta ccaaatcgct agcgaatgca tacaagcatt agaagctatc ttgcaacata
     4261 agatctctaa agtagagatc agctacctaa cgttgtattt ggagttagca tttcgatatc
     4321 aaaaagagca tttttcctct aaaaaagtag ccattgttca tacgacagga aaaggaacag
     4381 cattaatgat ccaaagacaa atcaaaagga tattggggag ggatatacaa ataacacatg
     4441 tttcagaaga acaatatgaa aaagtagatc taaacaaata tttcgctgtt tttacaacga
     4501 ttccgttaaa aaatgtaaat gaagagacac cggtgatcca tttgactaat ctttttgatg
     4561 actcttggtt aagggcacaa tggcaaaaag caaaaaaaaa gcgattagcc gggtttcatt
     4621 ctactgtttt tttctatcaa atatggaaag aaaaaaagac gtatcaagaa tatttaaaca
     4681 aattagtcgc tcctttgatc agaggctatt atgtggatga agaatttgaa aaaaggatct
     4741 tgaaaagaga atatcagcaa agcacatttg ttaacaaaga atttgctttt cctcacacaa
     4801 ccaaccagct atctcaacac attgttttat cagtagggct aataactgat caaggtttag
     4861 cagaaacagg aggaaatgga gttatttttt tatttgccat acctgaagaa atgacggctg
     4921 aagttgagaa tgaattattt gagctatacg atatcctatt tgaactcata aaaatgggaa
     4981 tagatgagtt tattgaatta gcaccgacac aagaatcatt tttaaaacta atcaaaagta
     5041 aaggagagag acttcatgaa tgatatgtat ctattcatag ttttcgcagc ttcagcttat
     5101 gtgctgcaag cagttatcag ttggaagcaa ctaaagcatt tcaatcaaac atatagcgaa
     5161 ctgcgaagaa aaggaaaagt agcgattggt aaacgacctg ggaaaatccg ttcaggcacg
     5221 attgttatgc tagctataga cgatcatgat caagttttag aagtcagaaa aatgcaaggg
     5281 gttacatctt tggcaaaatt tcattcatta cctgtctatg cgggtgaaga tatccattac
     5341 atcgatcgtt accatccctc agttcgagcg gaagatcgtt tgacacaagc agctttagaa
     5401 aatgccagag aagtatactt acgaatagag gcaggaaatg aaaaaagtga acaaccttca
     5461 ttgcctttta ttgctatgct caataatttt cggatatgga aaagtacgat caaagaaaaa
     5521 aggagtgaca aacgatggat atcataacaa aatttgctga gggatttatg aggctgttcc
     5581 aaacaggtgc agaaacattc ataagctgga tgacaggaat cgtaccagtt gtcttgatgc
     5641 tgatggttgc aatgaatacg ctgattgctt ttttaggtga agaccgagta acgaaagtag
     5701 ccaaattatc agcgaaaaat ccgcttactc gttaccttgt gttaccgttc ttatctgctt
     5761 ttatgttggg aaatcctatg tcatttacaa tggctcgttt ccttccagaa tactacaaac
     5821 caagctatta tgctgctcaa gctcaatttt gtcatacgag caatggtgta ttcccccata
     5881 ttaatcctgg agaattgttt gtatggatgg ggattgctca agggatcgaa accttagggc
     5941 taaattcgat ggagctggct atccgatact tgcttgtggg actgctgatg aactttattg
     6001 gtggttggat aactgatttt actactggat ttgtatgcag acagcaagga atcgttctta
     6061 gcaaaaaagt agagctttca gttgattaga gaaaggagag ttttaaatgt atggtgatat
     6121 taagatcgta agaggaaacg gcgggtatgg aggtcctttg attattaggc cgacaaaaga
     6181 taaacacaag ttcatctatg tcacaggtgg aggtgagagg ccaatgatcg tagataaaat
     6241 agaagaactg acaggtatgg agtctatcaa tgggttcaaa acatcaatac ctgatagtga
     6301 aatcgcgctt gcaatcgttg actgtggagg gactcttaga tgtggaattt atcctaaaaa
     6361 agggatacca accatcaata tcgtttccac aggaaaaagt ggcccattag cacaatatat
     6421 cactgaagat atttatgttt cagcagtagg gttagaacaa atacagccac taatggaaac
     6481 tgatgataac aaagaaacag taaatactgc atctccagat aaaaagtaca aatatgatac
     6541 ttctaaaaag ataaccgagc aaaaagcaga gcagcaaaac tggatagcaa aaattggaat
     6601 gggcgcggga aaagtagttg ctacattcaa tcaatcagca agagaagcag tagatacgat
     6661 gatccggaca gtgatccctt ttatggcttt cgtttccctt ttgattggtg tgatacaagg
     6721 ttctgggatc ggtaatgtat ttgctaacat tatggcacct ttagcaggaa atgcagttgg
     6781 attaacaatc attggcttta tttgttcact gccattttta tctcctttgc ttggtccagg
     6841 ggcagttatt agccaagtga ttggaacatt gatcggtgta gagattggga aaggaaatat
     6901 tcctccccaa ttagcattac cggcactatt tgctatcaat actcaaaatg catgtgattt
     6961 tattccagtc ggtctaggtt tggaagaagc aaaagctgag acagtagaag taggtgtacc
     7021 atcagttctt tattcaagat ttcttaacgg catcccacga gttatcattg cttgggtagc
     7081 aagttttgga ttatatagct g
//
LOCUS       DASJSZ010000115         6833 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.121, whole genome shotgun sequence.
ACCESSION   DASJSZ010000115 DASJSZ010000000
VERSION     DASJSZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.121"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            66..956
                     /locus_tag="U4B03_002557"
     CDS             66..956
                     /locus_tag="U4B03_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEN2069362.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1029..1853)
                     /gene="nadE"
                     /locus_tag="U4B03_002558"
     CDS             complement(1029..1853)
                     /gene="nadE"
                     /locus_tag="U4B03_002558"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEN2069363.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1865..3337)
                     /locus_tag="U4B03_002559"
     CDS             complement(1865..3337)
                     /locus_tag="U4B03_002559"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEN2069364.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3974..4336)
                     /locus_tag="U4B03_002560"
     CDS             complement(3974..4336)
                     /locus_tag="U4B03_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN2069365.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4559..5311
                     /locus_tag="U4B03_002561"
     CDS             4559..5311
                     /locus_tag="U4B03_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2069366.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5451..6374)
                     /locus_tag="U4B03_002562"
     CDS             complement(5451..6374)
                     /locus_tag="U4B03_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2069367.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6389..6661)
                     /locus_tag="U4B03_002563"
     CDS             complement(6389..6661)
                     /locus_tag="U4B03_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069368.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2094 a         1354 c         1163 g         2222 t
ORIGIN      
        1 tttaatataa cttttattaa caaatatgtt aatatataag aaaaaccttt taagaggtga
       61 tatatatgca actacaaata aacgaagaat ccttaccggt atatgaagca ttagctagta
      121 aaacaaggat caagatcatc cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg
      181 caaaagaact tggagtgagc agtgctatca cgacgatgca cgtgaaaaag ctagaggaag
      241 caaacatcat caaaacagaa aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata
      301 aaatcgatat cagttttcca gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga
      361 tacctattgg tcactataca aattatgcaa ttgaaccaac atgcggttta gctacgatcc
      421 atgattttat cgggaaagtg gatgaaccac gctattttat ggatcctcga aggatggatg
      481 cccgcattct ctggtttacc agcggatttg tcgagtatca ggcacctaat tttttaaata
      541 cagaggatac attggagatg ttggaagttt ctgtggaaat ttcttctgaa tttccttttt
      601 caaatgacaa ttggccttca gatatcactt tcagcctaaa tggtgtggag ctaggaacat
      661 ggacgagccc tggagatttt gctgatatcc gcggtaaata tacgcctgat tggtatccag
      721 ataatcttaa tcaatacggc ttgctgaaga cgatccgaat taccaaacac ttgacgaata
      781 tgaatggaga acccttgtca aacatcacga tcaacgacat ccctaaagag caagacactt
      841 ggcatttgcg aatcgaagtg aaagacgatg ccaaacatgt ggggggctgt actttatttg
      901 gtaaaggatt cggtaattac gatcaggata tcaaattaaa agtttactac agctgatatt
      961 tttcttttct attttataaa acaaaaacag ccaatcccta tgaagggacg gctgtttttg
     1021 ctttattgtt atttccagaa atcatccaaa acggaaattg gcaaatgtcg tttatgttgt
     1081 gtcttgttcc accatgcttc gattttttct tgtgcttgag cgggaacttc ttttccttct
     1141 agataatcat caatttcatt atatgtgaca cctagtgcga cttcatctgc aatcattggt
     1201 ttcccatctt caagatctgc agtcggaatt tttgtgtata atttttccgg tgcattcaat
     1261 gcttgaagca attgtttccc ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct
     1321 ccaccatcac caaatttcgt gaagaatcca gtaatgtttt ctgcagcatg atctgtccct
     1381 aatacggctc ctgctctttc tccagctacg gcatattgcg taatcatacg ttgacgtgct
     1441 ttgatatttc ctttgttaaa atcgctgatt cccacacctg cttcgcttaa tacttgtact
     1501 tgtgcatcaa cagctggttt gatatttact cgcagactaa catcgggctg gataaagtca
     1561 agagcagctt ttgcatcttc ttcatctgct tgttcaccat aaggaagacg aacagcgata
     1621 aattgatagt tttcatcttt tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa
     1681 cggcctgcta aagtagaatc ttggccgccg ctgataccta gtacaaaagt tttaagaaac
     1741 ggatattttt tcaaatatgc tttcataaag tcaatacttt tacgtatttc ttcttttgga
     1801 tcaattgttg gtttgacacc taattcattg ataatttctg tctgcaggct catttattct
     1861 cctcctatgc ttctgtatga agttctgcta cgcttttttt cattcgttcc atgatagcca
     1921 ttttatggtt ccagcattca gtagacagat cgactgggta tttttgcgga ttcaagtctc
     1981 gtttatactc ttcccatagt gaatctagat tttcttttgt atattctttg atttcgttta
     2041 gtgcaggtaa ttcgtaaact ctttttcctt cgataaagat atcttgtaaa actgggcgag
     2101 ctgtaaagtc tcgaacagtt ttattgataa acgtatgcac tggatggaac atgaagatag
     2161 cttcttcttc tcgagggtct tcatcccata atgtcacgta gtctccttca gatttcccat
     2221 caaaatttcg agtgatgcgc cacacttgtt ttttccctgg agtagttact ttttctgcat
     2281 tgcttgataa cttgatcgta tccatcattt ttccttcatc atcttcgatc gatactagct
     2341 tgaatactgc accaagggct ggctgatcat aggctgtgat caattttgtc ccaacacccc
     2401 atacatcaat tttagctttt tgcatcttta aattcaaaat cgttgcttca tccaagtcat
     2461 tcgaagcata gatttttgct tccgtaaatc cggctgcatc taattgttcc cgaacacgct
     2521 tagatatata ggccatatct ccactgtcga tgcgtacgcc ttggaagttg attttgtcac
     2581 ctaattcttt cgccacgcga atcgcacttg gcacacctga tttcaacgta tcataagtgt
     2641 cgactaagaa cacgcagtct ttatgggttt tagcgtaggc cataaatgct tcataatcat
     2701 ttccataaga ttggactaac gaatgagcat gggtcccgct agctggtatc ccaaatatct
     2761 tcccagcacg tacattgctt gttgcatctg ctccgccgat atatgccgct ctagttcccc
     2821 agactgctgc atccagttct tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc
     2881 cgatgacaga tttgatacga gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat
     2941 tcaacagtgc tgtttctact aattggcagt gcgctaaagg cccttccact tggattaatg
     3001 gttcatttgc aaaaactagt tctccttcac gagcagaacg aactgttgct ttaaattcaa
     3061 aattttgtaa atactccaaa aaaccttcag gatagacttc taatccacgc aagtaagcaa
     3121 tatcggaatc attgaacgtc agattctcta aataattgac taaccgttcc aatcctgcaa
     3181 aaaccgcgta tccgtggtta aaaggcattt ctcgaaagta acattcaaaa acagcatgta
     3241 aatcagctcg acctaattcc cagtatgtct gcatcatatt gatttgatat agatccgtat
     3301 gcagcgtcaa actgtcgtct gggtaagtat tattcatctt gtattactcc tcttggcaag
     3361 ttttcatttc ctacccatta taacaaaaaa aattaatagg aaaagaaatc ttccattttt
     3421 tctgaaaaag cacatgtttt acggatattt cttgctaaaa atgaactcca tctgctaaaa
     3481 ctatcctttc ttattggtat ttaacctaaa cgagaaagaa tacttatttt attcaatgta
     3541 tccgagacac gaagaaatta gcgttttcta tttttcttac ttgataaaat aagtagattt
     3601 tcctactaca atcgtagccg ttaagatacc gatgattgaa caaatataac caaatgtctc
     3661 tacaacataa tcaatggcaa aagtttttga agcaataata cgtctttcct tttttgcttc
     3721 ttatccccaa agaaacggat ataacttatt atgtattgaa tttttttgga aaggcgaatt
     3781 atgacaagat ataccttgcg ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa
     3841 agatacaaag taagaaagca gctgtcaata gaatggaaac aaaaatggcg agacatcaat
     3901 cagtctcgcc ttgtttttta ttgtatacat tagatatgga gcaggttacc ataaagatga
     3961 ttattagtcg ttattaaata gaatccttcg tatttttttg ttctatcact acaatatctt
     4021 tatttggacg tttgaattgt attccggcac gttctagctc ctgtttcact cctacatgga
     4081 ctttgacatc ttctcgtgtc atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc
     4141 ttgtgatttt tttcttcgta ttgatcatca cgacttgctt tttatcatat gtgtctacca
     4201 atagcagttg tgttcgatct aacgaaaata atttgtaata attaatagcc gtattcccaa
     4261 aaaagaattc cactacttta ggataaaatg tcttaatatt taaattacta ttcacaggtg
     4321 tttcgattag cttcatctca ttcctctttt ctatatagtc gatttagatc caatcttaag
     4381 ttatgttacc atctttccta gcattaagga atcggttaca attgaacctt taaagacaaa
     4441 aggatttacc ttttatcact taatagtata acataatttc gtgagaaaaa aatacaattt
     4501 tttcttgacc atctcgttac gtaaaggatt atgcttacat ttgtcaggag gaaaaataat
     4561 ggagtacaca ataaagaaaa tgtcagagat atcaggagtc agtccgagaa ccttgcgctt
     4621 ttatgatgag attggactgc taaaaccagc taggatcaat tcttcaggtt atcggattta
     4681 tggcaaaaaa gaagtcgatc gtctgcaaca catcttattt tatcgaacaa tggcattcaa
     4741 gttggatgat atccaagagg tcttagacaa tccatctttt gaccaccaga aagctttaat
     4801 caagcatcag caaatgttgc tggaaaaacg ggcacaaatc gataccctct tgacaactgt
     4861 ccaacagaca cttgatatgt acgaaggagg aagaaaaatg agtgacaaag aaaaatttga
     4921 agggtttaaa caacaaaagt tgaaagaaaa tgaagaatcc tatggacaag agattcgaca
     4981 aaaatacgga aaaaaaaagg ttacagcatc caatgaaaaa tggatgaata tggataaaga
     5041 aacgtatgaa gaaatgcaaa ctgttgaaaa gcagatgtta tttgatctca ctctttactt
     5101 aaaagaacca gaagatgaaa gcttagcaga ccgtatcttc caagctcata agaagtggtt
     5161 ctcttacagc tggcctgaat atcagccaaa agcgcataaa ggactaggaa tgatgtacga
     5221 agccgatgaa cggttcactg catattatga cgagcgaagt ggtcatggtg cagcaaaagc
     5281 tttaaatgaa attatccaaa aaaaggcgta atcttttaca tgatccaaca aaaaagtaca
     5341 cgtaaaatga cctaataaca aaacagcaga cacccaagtt agatttttat gctaactttt
     5401 ggtgtctgct ccattctttc acaagttttt tcatcctact ttaaatatat tcatcgttga
     5461 ataaacgtat aaggtatttt cactttatcc tttttttctg taagaaacaa ttgaaatgcc
     5521 atttctccgc aagcttccag atggtggtcg atggttggga aaccaagaag ctcgctgatc
     5581 agcaaattat cgcgtccaat caccactggt gtcttatctt gcagatagct ttgcaagatg
     5641 cctgctgcaa ccatatccga atttgtaaag acaccatcaa ctccaagctc tttaaaaaag
     5701 aagcctgctt gttttccttt ctctgcatct atacaatccc aaaaaacaag tgattcatca
     5761 aaagatggaa aaagttgatg acatagctgc aaagtgattt ttgagttata acttagcctg
     5821 ccacttcgcc ccaacgtgat gcccaattgc tttatacctt gctgcttcaa ataagtaaga
     5881 ccttctgtaa ttgattgttc tcgatcaata aagacacagc ttgcctgttt tcccttgatt
     5941 tcttcacaaa aaactagagg tccatatttt tgatatgcca ataaatcatc tagtgtattg
     6001 gcacgagacg taatgatcaa tccatcgaaa gctttagctg caaattcttc aagatacctt
     6061 cgctcttttt ctttatgata gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac
     6121 gctgcttcaa tgatcccact gatcaactga tcaaaatacg gatgattggt ataaggcaga
     6181 atcactccaa tgcttttcgt ttttccataa cttaactgtc tggcatttgc attagggata
     6241 taatcgactt ctttcatgac cgcaagtatt tctttccttt tttcctcatc tacatatgga
     6301 tgtccattga ttacacgtga aatagtagaa actgaataac ctgtgagctt tgcgatatct
     6361 cgaatgtttg ccactgctgc ttcgccacct atacaaaaag attttcttgt aaatagtcta
     6421 ccacagctgg accgcattca aaaaactgat acatttttcc ttcatgcgaa aaactgatcc
     6481 gctgcccgcc ccaatatata tcttttgtca aattttcgat ttcattcatt ggtatttctt
     6541 ttactatatt cctattcaac ccttgaaaac tgtacaacgt taatacggat cccttgaact
     6601 cgacaaacgc ataaaaataa ttgttcgttg tgccatctgt tatctctaca taagtattca
     6661 ttgcaagccc tccttttgat actttgagta tagtgtatga aacgcgtctc atagcaagga
     6721 gtttgttttg ccaaagaaaa gttttattcc tctttcatta ttaccaaagt ctcttatttt
     6781 aaattgaata cattataatg atactgtaaa tatatcttta ttattagtga atg
//
LOCUS       DASJSZ010000116         6525 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.122, whole genome shotgun sequence.
ACCESSION   DASJSZ010000116 DASJSZ010000000
VERSION     DASJSZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.122"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            41..778
                     /locus_tag="U4B03_002564"
     CDS             41..778
                     /locus_tag="U4B03_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2069369.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            792..1607
                     /locus_tag="U4B03_002565"
     CDS             792..1607
                     /locus_tag="U4B03_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN2069370.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1620..2267
                     /locus_tag="U4B03_002566"
     CDS             1620..2267
                     /locus_tag="U4B03_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2069371.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2283..2939
                     /locus_tag="U4B03_002567"
     CDS             2283..2939
                     /locus_tag="U4B03_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2069372.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3101..3922
                     /gene="racE"
                     /locus_tag="U4B03_002568"
     CDS             3101..3922
                     /gene="racE"
                     /locus_tag="U4B03_002568"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEN2069373.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3925..5280
                     /gene="rph"
                     /locus_tag="U4B03_002569"
     CDS             3925..5280
                     /gene="rph"
                     /locus_tag="U4B03_002569"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEN2069374.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5281..5799
                     /locus_tag="U4B03_002570"
     CDS             5281..5799
                     /locus_tag="U4B03_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2069375.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5906..6406
                     /locus_tag="U4B03_002571"
     CDS             5906..6406
                     /locus_tag="U4B03_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2069376.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2150 a         1095 c         1442 g         1838 t
ORIGIN      
        1 taaaagaaat cttactaaat aataatggga gtgtaattct atggcaatga ttaaatttga
       61 tcacgtggat aaatattatg gcaagttcca cgcattaaaa aatatcaatc tcgaattcga
      121 gaaaggagaa gttgtcgttg tcattggtcc ttctggttca ggaaagagca cgatgcttcg
      181 ctgtatcaat ggattagaga cgatctcttc aggtaagctg ctgattaatg atactgactt
      241 gcatgataaa aagacaaaac tgacagaagt ccgaaaaaac atcggcatgg ttttccaaca
      301 tttcaatttg tacccaaata aaactgttct ggaaaacatc aaattagcac caatcaaagt
      361 gttgaagcaa gaccaggcaa ctgcagtcaa aaacgcggag aaatttttga aaactgtcaa
      421 tatgttagat aagaaagatt cttatccttc catgttatcc gggggacaac agcaacgggt
      481 ggcaatcgcc agaggtttag cgatgaatcc tgagatgctg ctgtttgatg aacctacttc
      541 tgctttggat ccggaaatga ttggtgatgt attagacgtt atgaaaaaat tggctcatga
      601 cggtatgtca atgattgtcg tgacacatga aatgggcttt gccaaagaag tggcggatcg
      661 tgttatcttc atggctgatg gagaagtatt ggaagacagc agaaatgtga gagacttttt
      721 cgaaaatcca aatgaagtcc gagcacagca atttatcagt aaagttatca atcattgata
      781 ggaggaaaaa gatgcgcagg ttaaaaaaaa tacgcctgat tttcctttta ggcattgctt
      841 ttcttgcact agccggatgt aaaggaaatg ggttggcaaa tgaagatatt ttagagcgaa
      901 gcaaatctac caatgaaatc atctggggtg tcaagtatga tactcgatta tttggaatga
      961 tggatattga gagcagaact gtacaaggat ttgatgtaga tattgcaaaa gcaatcacta
     1021 aaaagatcct tggtgacaat ggaaaaacag aatttgttga agtcacttct aaaactcgga
     1081 ttccattatt gaaaaacgga aatatcgatg caattattgc aacgatgacg atcacagatg
     1141 aacgaaaaaa acaggttgac ttttcagatg tctatttcga tgctggccaa gctttgttgg
     1201 taaagaaagg aagccaaatc aaaagtgtag atgatttgaa tgcttctaca acggttctag
     1261 cagtcaaagg ttctacatcg gctgcgaata tccgacagca tgcaccagat gcaaaaattt
     1321 tagaattaga aaactatgca gaagcattta ctgctttaca atctggtcag ggagacgcga
     1381 tgactacgga taatgcgatt ttgttaggga ttgcagatga aaatccagag tatgagttag
     1441 ttggtggaac gtttactaat gagccttacg ggattgcgat caacaaaggg caagaaaact
     1501 tcttgaaagc tgttaaccaa gcgctagagg aaatgcatgc agatggcaca tacgataaaa
     1561 tctatcaaaa atggtttcca aatgaaactg aaggaaaagt agagtaaggg ggagaggcaa
     1621 tgagcgaatt gatacaaact tatggaccaa tctttgtcga cgggttcaag atcacattgt
     1681 tttcgagtgt cttagcactt gtgtttagtt tgattatcgg aacgttaatg gcaatcttcc
     1741 aattatcaaa gaataaagta gtgagcgcct tagcaaaggc atatgtagaa ttttttagaa
     1801 acattccatt attaatcatc gtgatgtttt tttatgttgt cgtaccgatt tattggttta
     1861 atctagatgg attccaagca ggtacgattg gattaacgat ctatacatct gcttttattg
     1921 ctgaaacagt acgttcaggg attcaaaccg taccaaaagg tcaaacagaa gcaggactat
     1981 cttctggttt tacctattca gaaatcatgc gttatattgt tttaccgcaa gcattcaaaa
     2041 tcgttatccc gccattaggc aaccaattta tcaacctcgt aaaaaactca tctattttag
     2101 caatcgtagc tggtttagat ttgatgtatc aaggagactt gatcgctagt gaaacattca
     2161 ataccttcga tacgtatatc atcgttgggt tattttactt agttatcacg ctaccgttat
     2221 cttacttaat ggtttatctt gagaaaaaat gggcggttcg ttcatagaag gaggggaaat
     2281 acatggattt tagcggcgct ttttcatgga tgaatatccg ttttttattg gaaggattaa
     2341 aggttactgt tgaagtttca ttgttttcga tcatttttag tttcttgatc ggtggtctga
     2401 caggtgtgat tcgttttgcg aatatccctt tcttttccaa aatccttggt gtagtgattg
     2461 atattatacg gaatcttcca ttattattga tcattttctt cacttacttt gccttacctc
     2521 aaatcggcat tcagatgaac atcttctggt ccgctgtcgc agcattaacg atttttgagt
     2581 cagctatgtt gtcggaaatt ttccgagctg gactcaatgc tgttccaaaa ggacagatgg
     2641 aagcaggatt atcaacggga ctgacttatg tagaatcgat gcgaacgatc gtgttaccgc
     2701 aagcatttaa atcaatgatc ccggcaattg tgagccaatt gatttcactg atcaaagata
     2761 cttcattagc agtaatcatt tctttacctg aactttctca tcaggcacgt attatttatg
     2821 gacagaacac caattatgtg cttccgatgt tcatcatcat gacacttatg tacttcgtgg
     2881 tatgttacgc gctttctctg ctttccggtt acttggaaaa gagacggtac agttattaat
     2941 agatgattct tttagcggtt gtggcaaact aaagtcacaa ccgcttttcc tctttccatt
     3001 cgaaaaaaag agatttcgat tgttttaagc aagtaagagc acttgaaaat tcacgctaaa
     3061 aagtacacaa tagggaggat agaatttatt gaaaaaaagg atgatacgat tgacagataa
     3121 tcgccctatc ggatttattg attcaggtgt cggcggcttg actgtagtaa aagaagccct
     3181 gaaacaatta ccgaatgaaa atattttatt tgtaggagac acagcacgct gcccatatgg
     3241 ccctagaccc gcggaacagg taatccagta tacttgggaa atgacggatt atctggtgga
     3301 gcaaggaatc aagatgctgg tgatcgcctg caataccgca actgcggtgg ctttagaaga
     3361 aatcaaagct gctctttcta ttccagtcat cggtgtgatc cttcccggta ctagagcggc
     3421 agtaaaaaaa acacaaaata aacaagttgg cattatcggt acgattggta cggtaaaaag
     3481 tcaagcttat gaaaaagcac tgaaagagaa agtaccagaa ttgactgtga caagtcttgc
     3541 ttgtcccaaa tttgtttcag ttgtcgaaag taatgaatac cattcatcgg tggcgaaaaa
     3601 aattgtggca gaaacattag ctcctttaac cactaaaaaa atcgatacat tgattttggg
     3661 atgcacccat tatccattat tacgccccat cattcaaaat gtaatgggag aaaatgttca
     3721 actgatcgat tctggagcag aaacagtagg tgaagtatct atgctgttag attatttcaa
     3781 tttgagcaat tcaccgcaaa atggtcggac attatgccag ttttatacaa ctggctctgc
     3841 caaacttttc gaggaaatag ctgaagactg gcttggaatc ggacacttaa atgtagaaca
     3901 tatcgaattg ggaggaaaat aaggatgcgt catgacggga gaaaagccca agagcttcgc
     3961 aaactggagc ttcaaacaaa tgttttcaaa tatccagaag gatcggtagt gatccgtttt
     4021 ggtgatacaa cagttatttg ttctgcaaca atagaaaatt ctgttccccc ttttttgaga
     4081 gaaacaggaa caggatgggt gacagcagaa tatagtatgc tgcctcgagc aacgagtacg
     4141 agaaatcgta gagaaagcag caaagggaaa ctttctggta gaacaatgga aatccagcgt
     4201 cttattggtc gttccttgcg tgcagtagta gacttggaaa aattaggaga acgtagtatc
     4261 atcgtagact gcgatgtgat ccaagcggat ggcggtacta gaacagcaag tatcactggg
     4321 gcatttgtgg cattgaaatt agccattgaa aaacttttaa gagaaaaaga attgtcagaa
     4381 gatccaataa aagaacatct tgctgcagtt agcgtaggaa tcttaccaga tgggacttgc
     4441 gtcacagatt tggattacca agaagattcc gcagcacttg tagacatgaa tcttgtaatg
     4501 acagaatctg gaaaattcgt ggagattcaa ggaactggag aagaagcaac gtttgatggt
     4561 gaacagttga atgaaatgct cttttttgga aaaaacgcga ttgaagattt gatcaaagag
     4621 caaaaacatg ctttgctaac agagtttgct caaaatgatg agcggataga agaaacaaaa
     4681 acaatcatca ttgcgacaag aaatccagga aaagcagaag agtttcgaaa tatgttcaaa
     4741 gaagcgggat atcatgtaaa aacattactt gactatcctg aattgccaga tgtggaagaa
     4801 actggcagca catttgaaga aaatgcccga ttaaaagcag aaacgattgc acaattgctt
     4861 gatcagccag tcttagcaga tgattcaggt ttgaaagtgg atgctttagg cggtatgcca
     4921 ggaatctatt ctgcacgttt tgcaggggaa caaaaaagtg atgcggggaa taacgccaaa
     4981 ttattatatg aattgacaga tgtgccggat gaaaaaagaa cagcacaatt ccattgtacg
     5041 ttagtattcg cggcacccaa aaaagacagc cttgtagtag aagcggaatg gcccggtcga
     5101 gtagcccgta taccatctgg cgaaaatggc tttgggtatg atcctttatt tatcccagaa
     5161 ggtaagaaac aaacagcagc agaattatcg agcgaagaaa aaaacaagat cagtcatcgg
     5221 gctcaagcaa tgaaaaaatt gagtgccgag tggaaacaat ggctggaagg agagagatag
     5281 atgcggtatt tagtagtaag tgataatcat ggagacagag atatcctcgt tgacttagta
     5341 gatcgttatc gttctgaagt ggatatcttt ttccattgtg gtgattcgga attagaagct
     5401 tctgacccat tatgggatac attccaagtg gttcaaggta attgtgatta tggaccagga
     5461 ttcgaacaga aaaaagtgat ccaaactggt caggatacag tattcatgac tcacggtcat
     5521 ttatcgaatg tccgatttgg tttgacacag ttagcaatcg aagctcgagc tgcaactgcc
     5581 gatatggtgt tttttggtca tacccatcaa ataggctgtg agatggatca gaaaatcctg
     5641 tttttgaatc ctggtagcat ttctcagcct agaggcccga tccaaatccc atcatatgct
     5701 cttatcgaaa gtttagatga ccaaatttct gttcaatact ataaccgtgc ccatcaggca
     5761 gttgaagagt tggccttttc tttttcgaga gagcgctgaa taaaaaaact ctaattttta
     5821 ataaaaataa gctttatttt atgaattttg catccggttt cggtaaaata gtagtgttga
     5881 atgaaataga aatggaggcc atcagatgat tggacctata gtaagagaaa tgctgctgga
     5941 aaatcaggaa acgtttttga ttcctgcaga aaatgtggca aatgttatgt atcatcatcc
     6001 attgtcccat ggtttacttg tgttatcgaa ggtcggttat tccaaaattc ctgtattagg
     6061 aaaagatgat cactttgtag gactagtaag tttatcagat gtggtcaata aaatgattga
     6121 cttgcaaaca atcagcatgg acccgttaga aggactaacg gttgctgatg tgacagaaac
     6181 agatgttccg acaattgatg aaaattggga actagaagaa gtactccatt tgttagtcga
     6241 ttcgcctttt ttaccggttg tcacagaaga tcggattttc aaaggaatca ttacaagaaa
     6301 agagctgctt aaagccgtaa acttcatggt gcatgaatta gaacgtcgta atatattgtc
     6361 tccgaaaatc gaagaattaa aagaacaaat cgatatcgtg agctaaagaa cgagaaacta
     6421 aaagataatc aaaaaataag tagaaaaaac agcgtaccag ttttgacttt atcttgaaaa
     6481 agaggctggg acaaaagcgt ttaactccaa gaaataagaa ggaat
//
LOCUS       DASJSZ010000117         6402 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.123, whole genome shotgun sequence.
ACCESSION   DASJSZ010000117 DASJSZ010000000
VERSION     DASJSZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.123"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            578..1183
                     /locus_tag="U4B03_002572"
     CDS             578..1183
                     /locus_tag="U4B03_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEN2069377.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="U4B03_002573"
     CDS             1199..1771
                     /locus_tag="U4B03_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2069378.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="U4B03_002574"
     CDS             complement(2023..2280)
                     /locus_tag="U4B03_002574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069379.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="U4B03_002575"
     CDS             complement(2406..3221)
                     /locus_tag="U4B03_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069380.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="U4B03_002576"
     CDS             3738..5063
                     /locus_tag="U4B03_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN2069381.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="U4B03_002577"
     CDS             5056..5406
                     /locus_tag="U4B03_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069382.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5388..5573
                     /locus_tag="U4B03_002578"
     CDS             5388..5573
                     /locus_tag="U4B03_002578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069383.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
BASE COUNT         2206 a          985 c         1175 g         2036 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa gg
//
LOCUS       DASJSZ010000118         6387 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.124, whole genome shotgun sequence.
ACCESSION   DASJSZ010000118 DASJSZ010000000
VERSION     DASJSZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.124"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            330..1175
                     /locus_tag="U4B03_002579"
     CDS             330..1175
                     /locus_tag="U4B03_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2069384.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="U4B03_002580"
     CDS             1456..2676
                     /locus_tag="U4B03_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEN2069385.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="U4B03_002581"
     CDS             2673..5393
                     /locus_tag="U4B03_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2069386.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="U4B03_002582"
     CDS             5377..6321
                     /locus_tag="U4B03_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2069387.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2379 a          987 c         1231 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DASJSZ010000119         6314 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.125, whole genome shotgun sequence.
ACCESSION   DASJSZ010000119 DASJSZ010000000
VERSION     DASJSZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.125"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(89..1219)
                     /locus_tag="U4B03_002583"
                     /pseudo
     CDS             complement(89..1219)
                     /locus_tag="U4B03_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1279..1506)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1868..3070)
                     /locus_tag="U4B03_002584"
     CDS             complement(1868..3070)
                     /locus_tag="U4B03_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2069388.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3130..3333)
                     /locus_tag="U4B03_002585"
     CDS             complement(3130..3333)
                     /locus_tag="U4B03_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEN2069389.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3475..4707)
                     /locus_tag="U4B03_002586"
                     /pseudo
     CDS             complement(3475..4707)
                     /locus_tag="U4B03_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4870..5682
                     /locus_tag="U4B03_002587"
     CDS             4870..5682
                     /locus_tag="U4B03_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069390.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1873 a         1298 c          997 g         2146 t
ORIGIN      
        1 tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg actttttggg
       61 acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg acttgctgca
      121 ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct tctgttgaag
      181 caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt tgtgcttctt
      241 ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa gtaactaatc
      301 ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa tctcctgctc
      361 ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac tcttctgcaa
      421 tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg cagatatgtg
      481 ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc tctgtacaaa
      541 tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt ttagtttgat
      601 cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct ttttcataaa
      661 aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca gatagatcag
      721 gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt cgtaaaatca
      781 tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc actacttctt
      841 tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat ttatctacta
      901 cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat ccagtttctg
      961 gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa aatccacgcc
     1021 gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc actaattttt
     1081 ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct tcttgaactt
     1141 tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga tctactgtaa
     1201 atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa aaaaaatcct
     1261 tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt ttttcagaaa
     1321 aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga aagactgttc
     1381 gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt caacttctag
     1441 ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc ttctcatcgg
     1501 atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag ggaataagaa
     1561 caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat aaagcttttc
     1621 taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa atgatcatac
     1681 ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa aactaagtcc
     1741 tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac atcttgaaca
     1801 ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg gtagaaccgt
     1861 ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc tcttgaacat
     1921 tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg taagttccta
     1981 aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt tgaatattct
     2041 gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc ggtttgacac
     2101 ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc gtaaatgttc
     2161 cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca taccactgtc
     2221 tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat ttagttttgg
     2281 gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca atcgttatcg
     2341 tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca atccgaatgc
     2401 cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc ttatcatcct
     2461 tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact ttcttattct
     2521 cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc tctttaacag
     2581 cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat tttgataacg
     2641 tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt tcaaataacc
     2701 aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa aactgcatac
     2761 gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt ctctcgtacc
     2821 attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta gaacacagtt
     2881 gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa gcaattttgg
     2941 catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca ttttcatcta
     3001 tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa atatttttaa
     3061 tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat agtaccgcgc
     3121 ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc gggatggcac
     3181 cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa gatatgcctt
     3241 gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta tcaaaattgc
     3301 acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt ctttactcct
     3361 tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt tatactcgtc
     3421 ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc ttttttagta
     3481 gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa cctcctcctg
     3541 ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg gattcatttc
     3601 tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga cctgtgcaat
     3661 aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct caaaactttg
     3721 aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg ctgcccattt
     3781 ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa ttgctccatt
     3841 aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct ttgccaagct
     3901 ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg cctcttctaa
     3961 aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa aacgacactg
     4021 actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta tgatatccgt
     4081 accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta ttaccttatg
     4141 cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat caatccgtgt
     4201 aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg tttttcgtgt
     4261 ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca cagttccttt
     4321 aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt attggccaat
     4381 atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt caataatctc
     4441 ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat caatcaaaac
     4501 acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca atttttcttc
     4561 acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa tccccacttt
     4621 ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa tggcttcacc
     4681 gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt acttgtcaaa
     4741 gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta ttttaagtca
     4801 atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt atcctgagga
     4861 ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca gattcgccaa
     4921 gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc gcgaagtaca
     4981 gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca ccaaatcgca
     5041 gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa tcaatacatt
     5101 ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg tctcatagta
     5161 gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat gattgaattt
     5221 tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca atgtatcgat
     5281 gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca gctctatttg
     5341 caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc caactttagt
     5401 gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt tgggaaacat
     5461 ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca attgaccagc
     5521 gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca gtacacagag
     5581 gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat ggaagtcgag
     5641 aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa aattgataag
     5701 cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca cagtttcctg
     5761 ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg ctacgccttc
     5821 ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg acctggagtt
     5881 tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc ataatcagcg
     5941 cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct ggttagttaa
     6001 tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc aacctttaaa
     6061 aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca ttctcaaatt
     6121 ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat ttccattttt
     6181 gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg aaaaattttt
     6241 gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct caaattattt
     6301 ttctctaata agaa
//
LOCUS       DASJSZ010000120         6265 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.126, whole genome shotgun sequence.
ACCESSION   DASJSZ010000120 DASJSZ010000000
VERSION     DASJSZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.126"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(42..230)
                     /locus_tag="U4B03_002588"
     CDS             complement(42..230)
                     /locus_tag="U4B03_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEN2069391.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="U4B03_002589"
     CDS             500..862
                     /locus_tag="U4B03_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2069392.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="U4B03_002590"
     CDS             914..2596
                     /locus_tag="U4B03_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEN2069393.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="U4B03_002591"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="U4B03_002591"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEN2069394.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="U4B03_002592"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="U4B03_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEN2069395.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="U4B03_002593"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="U4B03_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN2069396.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2079 a         1091 c         1430 g         1665 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaaggatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctgg
//
LOCUS       DASJSZ010000121         6177 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.127, whole genome shotgun sequence.
ACCESSION   DASJSZ010000121 DASJSZ010000000
VERSION     DASJSZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.127"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..1378)
                     /gene="celB"
                     /locus_tag="U4B03_002594"
     CDS             complement(26..1378)
                     /gene="celB"
                     /locus_tag="U4B03_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN2069397.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1378..1896)
                     /locus_tag="U4B03_002595"
     CDS             complement(1378..1896)
                     /locus_tag="U4B03_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069398.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1912..2238)
                     /locus_tag="U4B03_002596"
     CDS             complement(1912..2238)
                     /locus_tag="U4B03_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEN2069399.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2251..4230)
                     /locus_tag="U4B03_002597"
     CDS             complement(2251..4230)
                     /locus_tag="U4B03_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2069400.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4251..4565)
                     /locus_tag="U4B03_002598"
     CDS             complement(4251..4565)
                     /locus_tag="U4B03_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEN2069401.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4733..5026
                     /locus_tag="U4B03_002599"
     CDS             4733..5026
                     /locus_tag="U4B03_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEN2069402.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5104..6174)
                     /locus_tag="U4B03_002600"
     CDS             complement(5104..6174)
                     /locus_tag="U4B03_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2069403.1"
                     /translation="MDFYQSLQLDPFILKQKIREAASKKEKCMYICSLFLRSLFIVLF
                     AICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSIIGLAIVMFSLLVAPY
                     VQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFIVYSLPKDSLNKNFF
                     TQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQEKIWMLIYAFGISLL
                     IVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGSLIGCVLFIGISQFI
                     PFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAVTIRIFENILGVCLG
                     IMYIGVTEILIRKIREKHGLNH"
BASE COUNT         1989 a         1263 c         1001 g         1924 t
ORIGIN      
        1 gtcacagttc cttttttctt agaaatcatt ttaacttttc actagctaat tctgctgcct
       61 tttctctttc ttcatttaat aatttattat catagaattt gataaatggg aaccagataa
      121 tgaatgcgat gattgcacaa atgactgcta acacagcagc tttccaatca ccaccactac
      181 caacaaatgc tccgatcccg actggtgaag gccacggcat ctgtgcaagc atcggtttga
      241 cgatttccag ccgaatggca aaataagcga gtgtagcaga agccatcgga gctaagaaaa
      301 acggaaccgc taaataggga ttgtagacga ttggcatccc gaaaatgatt ggttcattga
      361 tattgaataa tcctggtaca agtgaggctt ttcctaacac tttcagctga tcagatttcg
      421 cgaggaatgc aataaagatg atcaatccta aagtcgcacc tgatccacca acggttacat
      481 aagaattgtt gaattctcct gccaacggat aatttgcgcc ttgtgcgtta gctgccatat
      541 tagctaatac gatcggggtc aagaaagaag tgatgatatt cgctccatgg atccctacga
      601 tccacaatgc atggatcaag aaataaatca ccatgacacc aagccatgta ttcgtcaact
      661 gcgtaacaaa cccaaatggg atagcaatca ttttgaagat atccgttcct agtgcaacta
      721 ataatccatt gatgatcatc accacaaacg caatgacaaa tgttgggatc agagcagtga
      781 aagaacgaga aactcctggc ggaacaacat ctggcatttt tatgacccaa tttctcttga
      841 cacacataca atacaattcg accgcaataa tcgacataat aatagctgta aagattcctg
      901 aagttcctaa acgttcgaca aaagagccca tcctaatacc atttactaca gtttctgtct
      961 cattgataga agcaatcaag ctgattttac catcagtcat gatcaattca gggatacaca
     1021 taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg attaacattt aatttttgtt
     1081 catctcgttc aattgacgtt aattcataag caaacacaat ggcaaagtaa atagataaaa
     1141 tccccatcgt cattgtattg acgatcatgt acaagtcact aactttgaaa aaagttgcat
     1201 taaaaaatcc ttctaaaaat gggaacggta atggcaaaat atttaaaacc aaaaacatcg
     1261 agcctacaat cgtgaaagga atagaagcca tccccgctgc catgacagcc cgaacgattt
     1321 taagttgcgc aattttcccc ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta
     1381 gttattctcc tttcctaaat ataacgcttt ttcatattgt ttaatacgga tatactgttg
     1441 gtcttggttg agtgaaattt tctttaaacg atactgtata aatgcactgg caaaaatacc
     1501 gcttacaaca agaatgaaaa caaataactg tcctccattg ttatttgcca taaaaggata
     1561 caagaatgta aatcctgagg taaacaacac tggaatcact aataaattaa agacactctg
     1621 gaacaaaaaa taccactttg tccatggaac gttcgtttgg tgttttcgat aaagctttac
     1681 ttgttctcct actgcaggca aaatagctaa aatcagaatc atcggtaaac tacttcctaa
     1741 acttttagct gatagaagca gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt
     1801 caggtaacgt acgattagat aacgattgaa atacatgttt ttcaaactaa gttgcttttt
     1861 ttctaattcg attttccttt ttactgtatc ctgcacaatg atccttcctt tctatttatt
     1921 tttttgatac aaataattca tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag
     1981 atgatcttgt gcatggacca tgataatttc catttctatt tttgtcccgc tggcatattc
     2041 tttcagaagt tgagtttgtg actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa
     2101 cttctcttct acttcttcaa acatctcttt acgcatcgag cgaaatgctt catgtatcag
     2161 tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact
     2221 gtttattttt tcagccatct gttttcctcc ttattcgatc agtttgataa atttactgct
     2281 gaaattctca aaaataggtt cagctaagat ttcctgctgt acttttagct ggtcaactaa
     2341 cctaacgatt gctttagtca cgaccgtaat tccttcattt tcgacataag aaggcgaaac
     2401 taaaaaaaca aaatgaattt cttgatattg atcccactgc atcccagaag gaatcaatgc
     2461 aacggctacc tttgtttcga ttccaactgg aacagcggga tgtgggactg ccactgtatc
     2521 actgaatatg atttgtccca tattttcacg ttgctggatc tgctttcgca tttctcttat
     2581 atatccttct tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt
     2641 tttcggttcg tacgtataag tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg
     2701 ctttttttgc tgtagagaaa atgctggtat cggtctatct gaggaatgat agggtataga
     2761 ttctccaact acctttcgta ttttacgaac atcatcttca ttcaaaaaaa tactgacatg
     2821 taaaactgga attttgaaaa acattgtcga aagatcaata gaagaaataa tcaggtcgat
     2881 tccttttaaa gttgtttcat tgatctcata atagcctttt acacttacca ctgtaatatt
     2941 tttaccgaat tcactgacta cgcgattctt taatagttgg gcactgccgt aacccgttgc
     3001 acagataatc aatgcatgta gtttatgcgc agctctttct ttttccaatg ccgccatgag
     3061 atgcaaagcc aaataggccc attcatcttc attgatggta tagtttttca aagccggcat
     3121 gttgctgaaa tgccttttta ctgcgtcaaa gactttggga ttttctttct ttatctcctc
     3181 agtcaatggg ttctcaatgc gaatacctcg gctcaatcga atcatcatcg gtgacaaatg
     3241 atcgagtagt ccattgaaaa gctggagatc ctctactaat gaaaaatgaa attcctgaga
     3301 caatcttttc agaactttcg ctaattcctt tgatagttcc tgatcatttt gattttcttt
     3361 gtgattcgac ttagccatga gatgtaatgt caaataagca atttcttctt cgggaaacga
     3421 tacacctgcg attacctcaa tacgttgaac gatccgctgc gccacttgat actctactcg
     3481 cttagaaact tcttgttcga tgcctaattc cttgatttca aacccttctc ggagccgttt
     3541 gatccccaaa gccaagtgta aagtcagatt ctgtatgata aaatccgaaa cttttaactt
     3601 agcttctctt gtttcatcga gaatgataat gactagctct tcaaaactga tgtctgggaa
     3661 aaaacggctt tgccctaaat attctttcaa tgaatttcca taatggtttc caaaaaaagt
     3721 atccatgata aaatgccgtc gatttctttc ttccccttcg atccatgtcc cttgaccatg
     3781 cttagaaacg atttttagtg aatatggaat caatcgctcc ttgatttctt gcatgtcctt
     3841 tgtcaaactg gaacgactga tataaagctc ttctgccaaa tcatccataa aaagaatggc
     3901 atcttccaaa agcaattggt tcaatatata cttttgtcga tcttcggact cataaaatcc
     3961 ttgcggttct ctagcatatc caggtactat ttcttgccga ctcaaaaaca agtcaaatgt
     4021 cagtctttgt aaaatatgca gttggtagcc ataacctggt ttagcaatga tcttacctcc
     4081 gtttttttct actaactctt tgatccgctg caagtaatta cggattgtcc gatcagataa
     4141 taacaattca gaagctaaac gttgactggt gacatactcc ttcttgtgat caatcaaata
     4201 tctcaacagc aatttttcct tctgtttcat tatcatccac tcctattcca tcatatgttc
     4261 tcttcgatca cctctgctaa cttctggatg cctgtaggga ttgggacata cgcttggaaa
     4321 gggatactaa caacaggctt tccaacagat tctcctaatt ttttgaattt atccaaaaac
     4381 atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac tattcttaat catattatcg
     4441 ccttctgtag ctgaaactgc atctacgagt acctcttctc ttttttgctt aaagaaatcc
     4501 gttgtctttg aggccatcat cgaggatgac attcctgccg cacaaataat caatgctttt
     4561 ttcataaatc aatcactcct tttattttta taaaccaagc atatcttgta agcgttttct
     4621 attcaaaata tatatttccg tagcattgcg gaaaaaatgt gtatcatatc ttgaataata
     4681 atttctggca ccgtgatata ctagtgatag aattttttca ggaggggatt tagtgagtat
     4741 cacagtgaat attttgtata caagtacaaa tggacaagcc agaaaatttg cagaagaaat
     4801 ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa ggaaacgaga aatatgacta
     4861 ttactttcca ctagaagatc cagaatctct tttactgatc gaccgttgga aagatgaaga
     4921 agcaatcgaa aagcatcaca aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa
     4981 gtataaatta aaaatgaaag tcgaacgttt cactgattta ccgtaaaaag atacacatgg
     5041 aatgatagca atttatcatc catgtgtatc ttcacttttt ccttattttt tattcgatct
     5101 catctaatga ttcaaaccgt gtttttctct tatttttcgg ataagtattt cagtcacccc
     5161 aatatacatg attcctaaac ataccccaag gatattctca aaaatgcgga tcgttactgc
     5221 tcctggaacc ccgaataaac tggcagtgat tgccaaagca ccaaaagaat taaaaactgt
     5281 tttatatcta taggtagaac aaacacctaa agctagacca cctaatatac cgacccagtt
     5341 aaaaggaata aattggctga taccaataaa taaaacacat ccaatcaatg agccaatgat
     5401 tcggtctact gctcgttctt tgaaaccaat cgacatcaat ccatagctag atacaataga
     5461 agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg acatactctc ctgcaacgat
     5521 caaaagacta atgccaaatg catagatgag catccaaatt ttttcctgcg aatacatgcc
     5581 tttcaaaaaa atctctttaa acaagctttt gtctttgttc ttttctcgat gttttctgta
     5641 aaggagaact gaaaaccaac aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa
     5701 attcttgttc aacgaatctt taggtaacga ataaacaata aagagatatg aaaagccgta
     5761 caaagaagca ttccccattt tgggatcact agcggttgcc attaatatgc tgcttagtaa
     5821 aataaaatga atcaacatgc ccataactga ccatttgatc agctggacat acggtgctac
     5881 taatagtgag aacatgacga ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa
     5941 gtcaacaaaa cgaatactca tcaacataca gaataaaacc acagcgtatg gtttatgctt
     6001 cgattcaaat aatgttgtaa ttataatgat aaaacaaatg gcaaacaata cgatgaataa
     6061 tgatcgtaaa aacaatgaac aaatatacat gcacttttct tttttagaag ctgcttctcg
     6121 tattttttgt tttaaaataa acggatctag ttgtagagac tgataaaaat ccacctg
//
LOCUS       DASJSZ010000122         6157 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.128, whole genome shotgun sequence.
ACCESSION   DASJSZ010000122 DASJSZ010000000
VERSION     DASJSZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.128"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..229)
                     /locus_tag="U4B03_002601"
     CDS             complement(<1..229)
                     /locus_tag="U4B03_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2069404.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVS"
     gene            548..1273
                     /locus_tag="U4B03_002602"
     CDS             548..1273
                     /locus_tag="U4B03_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069405.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            1263..1772
                     /locus_tag="U4B03_002603"
     CDS             1263..1772
                     /locus_tag="U4B03_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069406.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1842..3290)
                     /locus_tag="U4B03_002604"
     CDS             complement(1842..3290)
                     /locus_tag="U4B03_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2069407.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3290..4006)
                     /locus_tag="U4B03_002605"
     CDS             complement(3290..4006)
                     /locus_tag="U4B03_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2069408.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3987..4337)
                     /locus_tag="U4B03_002606"
     CDS             complement(3987..4337)
                     /locus_tag="U4B03_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN2069409.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4481..5254
                     /locus_tag="U4B03_002607"
     CDS             4481..5254
                     /locus_tag="U4B03_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2069410.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5425..5791
                     /gene="ssrA"
                     /locus_tag="U4B03_002608"
     tmRNA           5425..5791
                     /gene="ssrA"
                     /locus_tag="U4B03_002608"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1804 a         1198 c         1068 g         2087 t
ORIGIN      
        1 cagacacatc attcagtaac aaccgaacgg ttgtcgtccc ccaacgaatg atcctagagg
       61 ggacgacaca aagacaatgg gaacactctt ctggcttgta cagaagagtt cctttgtaaa
      121 tcaagcaaat ttgccctttg tatttttctt tagttaaaca attttcctcg aaatagatat
      181 ttaggtctag aatatttagg gtatctttga taagctgagt atgagacatc ttgaatcctc
      241 ctatgaattg gtagccaaat taattctaaa ggatttcagg gtgtcttttc tatatttttt
      301 tatacaaaaa agatgttggt gaagttcacc aacatcaaaa agtatagagc ccaaaattat
      361 tgtcgctgct cctcttttcg aataaacagt gttcaaaagc ttgcatctac ggtaatagaa
      421 tagggcagct tcttcacaac ctcttgcttt ttacaaaata ttttctggtt ttaattcgtg
      481 atttcactaa aggaatttca tactttattc atattcattt tctatacttt ttatagaggt
      541 gaaaagtatg aaaataatcc gctatgctac tgctcgatta atttattaca aaaagcagac
      601 aatactctat tgtttatttt caagcttttc tgctttttta ttgatagcat gcctgactct
      661 ttaccattcg caatcggctt tagccaatca agtccaagat cggctttcat ttattggcgc
      721 ttctgcatct acagagcttc ttccggcttt agaattagct agccgatttt atataaaagg
      781 catattcagt ttattttttc tatttgcctt cctttttatc tttttctttt actattcagt
      841 caggcaaaac aagcaggaat tgatcaattg gcgcctgact ggtttttcta aaaccaaatt
      901 attccttttt attttctggc agctgttctt tcctctcttt ctttgttgta tcttggtttt
      961 attgtggatc gtcagcttcc agtcagttta tgaagcgctt cttcaaagga tcaattttct
     1021 tgtcttaaag actactgaac tgccagatat ccgtgatatc ttgacttcgg taagcggact
     1081 tacaattccg atggatcaac agactttttt taccatcgat ttccaaaatg atcttttttt
     1141 cacagatata cttaagggat ttgcccaaac tcttattggg ctcagtggat gctgtctcat
     1201 tcttagtcta ttccctttca ctttttttat ccatcatttg caaagtagga ggcctggacg
     1261 taatgaaacc tgaatatttg attcatgctg aacacttgac cgttattttg tgtcctctcg
     1321 aaaaacagca taaaatattg gagcacttga aaaaagaggt tcttgttgca aaactggata
     1381 attttgcgat catccaaaaa aattggccga tgtttcctta tctgaatctt aaagaccatg
     1441 tcctgttgga tgtaccggaa aaagaaatca aacaagatcg tttggcctat caagaaaaac
     1501 ttgatatctc tccttccttg cttaattgtg cggcagctga gttgacgtct tttgaaaaag
     1561 taaagctgca attacttcat gccttgcttt ccaagcgaaa caatattgtt attgaagata
     1621 catttgatga acttagtgta ttggagatcc aagagctact ccacttgctt tcttatttag
     1681 ctcatgaaga aaatcaaggt atcttactct ttactcatga tgctactatt gctcaaagtc
     1741 cttatattga tcgtttagag cccgcgggct aaatcaatca ttttatagta aaaacaagcg
     1801 gtatagaaga tcgattcatg atcttttata ccgcttgttt tttactcttt tttgatttct
     1861 acgtcaggca aacgaacagc gaattttgtt ccttttccta gttcgctttc tacttctaat
     1921 tttcctttat gcaagcgtac aagttgctgc acgattggta aacctaatcc tgattcacca
     1981 tattttgtat tttttcttga aggatctact ttataataac gatcccagat acttttcatt
     2041 tgttgttcag acattccaat tcccgtatct tcaatcgtga tgatcgtttc aagatagcct
     2101 ttttctaaag taatcgtgat ttggccgttt tcagtaaact gtatcgcgtt ttgaataata
     2161 ttgaccatga tttggacaaa tcggtcataa tcagcgtaaa catcaatatc atcaacggct
     2221 ttcaatgtca atgtatctcc tgctgcttct gctttagctt ctaactgtgc aacaatattt
     2281 ttcaatgttt ctgttccatt gaatttttta atgaccatac tgatttgatt cgtacggatt
     2341 ttttcataat ccaagttttc attcaccaaa cggatcagac gctctgtttc atttttcatc
     2401 aatttaatcg cgttttctcg ctgattttca gggattgcat tgtattctaa cccttccaga
     2461 agtccgttga ttgtcgtaag aggtgttctc atctcgtgag aagcatctgc cataaattgt
     2521 cttcgacgat tttcttgttc ttcaatttct gcttgagatt ctttcaatga attcgtcatt
     2581 ttattgaaat catctgctaa ctcatcaaat tcatctttgt catgtacggg gagctgtaca
     2641 tcaaaattac cattcgtcac ttctttcgtc gcattcctta accgattgat acgcttaact
     2701 tggaatgctg caaaagcata gctggcaata actgcaacga tactggaaaa aataaatcct
     2761 ttgaacaggt tcagtgtaat tggacgaacg ctgttatcga tatttcgcgc aggttgacta
     2821 actacaagtg ctccatagaa ttcatgattc aaattaaatg gcacaagtgc gtaagaggtc
     2881 gcctgtgctt gtcctaaaat atttttattc gatgtgaatt tttcttgacg tccattcctc
     2941 agatttttcc attgatcagc tgttaaggta aaattcagta aagctgtatt agtcggataa
     3001 atgacacgtt cgtttttatc gataaatata aagttcacat tttgttctgt cagtacttgc
     3061 tctgtcagca gcaatgcatt ttgaaacgcc tgatcttgac taaactgcgg gagcgtatcg
     3121 gcataggttt gcgttgtctt ttcaactgat tcagcatatc caaacagttg ctggtaattg
     3181 ttttcttcaa tcgtttgttt tgtcagttgt gtaaaagaaa ctccaacgat caataagatc
     3241 aacgcgacca ttccgcaaaa agctaagagt tgctgataca aataacgcat taggcaacac
     3301 cagaatcatc gaatttgtag ccgactcccc aaacagtttg gatgacttgt ggtccgacct
     3361 tttcgatttt ttgtcgtaat ttcttgatat gggcgtccac tgtccgttca tcaccaaagt
     3421 actgataatc ccagactaat tcaagcaact gctcccgaga gaatacttga cgtggttttt
     3481 ttgctaaagt aaacaataga tcgaactctt taggcgtcag tccttcaatt ggccggttgt
     3541 ccagatacgc ttctcgtgtt ttcgtattca tcttgaaatg actagtctcg atatcaaaat
     3601 gatcatctga aactgctgct tcttctcttg cttctgccaa ctcactacgg cgatgcaaag
     3661 ctttcattcg agcaataagc gtcagaggac tgaaaggttt tgtgacataa tcatctgccc
     3721 ccatctctaa gccgatcacc tgatcgcttt cagaatctct agctgtcagc atgataatcg
     3781 gcacagtact tgaaactttg cggatctctc ttcctactag cataccgtcc agtgtgggca
     3841 aattcaggtc tagtgtaatc atatcccaat gttgtgggtt ttctaaaaag gcatcgagcc
     3901 cttcttttcc atcatacttg aaagttgctt cccaaccttc atttaagaaa aacatctgca
     3961 tcatttcaga aacagattga ttatcttcaa tcattaaaat attcattatc gttccctcca
     4021 gtttccaggt catatttctg taatttcttt tctgtcataa taaaaatagg cataattgga
     4081 aatactagca gaagcatttc aatcagaaaa taattcgtat gcccagatgt ctttaaataa
     4141 tagccgatca atgccatcag taagcccata agaaaaaacc agtttccgct tgtcttttga
     4201 gccagccgcc aatgagcgtc tgtttttgcg gcaagaggcg aatgataccc ataaaaaggc
     4261 agatttcctt ttgagggcag aatccgaaaa agactgccaa ttattaagaa taagatacca
     4321 atactgaaaa aaatcattct ttccctccta aattctcttc ttatagtata ctctaaattg
     4381 ttgactgaac caatatgttc aggaattctt cacgaaaggt ttgtcttttg ttcattcttt
     4441 gttaagataa acttattcca ataaaaaagg agttttattt gtgtcaaatt atcgtgcaat
     4501 cactttcttt gatcttgatg gaacattact agatgcgaaa tcaaaaatca cacccgaagt
     4561 ggcaaaagct atgaacgcat taaaagaaaa caatgttctc cccgtcatcg ctacaggacg
     4621 tacagaagca gaaatccact ctatcatgga agatgcgggc attacttctg cggtcaccat
     4681 gaatggttct ttcattcgag tagacggcga agaaatctat tctcaaacat tttcttcaga
     4741 agaatgtcaa aaaatgtatg atcatgtaca gttgaacggt catgaacttt cttactataa
     4801 cgatcagaaa atctggtgta ccggtcatag ccaaacaatg atcaatgctt acgacttcat
     4861 tcattcagat gttccggaaa ttgatccttt aggctttaaa gaaaacacag taaatatgtt
     4921 gctgatctta tctcaatcag gtgacgaata ctatcacgaa cattttcctg agctaacttt
     4981 ttatcggaat ggtccttact cgatcgatac agtgaagaaa ggaatctcaa aaggggcagg
     5041 tgtcaaacgt ctgcaaaaag aacttcagct ggaaaaagtt cctacttttg gatttggcga
     5101 tggtccgaat gattttgcct tgttggaagc ttgcgacaac aaaatcgcta tggacaacgc
     5161 ctatgacgga ttaaaagaac tttctacctt tatcacaaag aaaaatacag aaggcggaat
     5221 tgtccacgca ttgaagtatt ttgatctgat ttaagatctt tttttgaagc tacctctttt
     5281 cttaagaaaa tcgcttattt tttcttaaga aaggggcagt tattatataa ataatgggat
     5341 atgattcgct tttttattga atttatccca atatatgcta tactaatttt gtatgaaaaa
     5401 tacacgtaac ccttgttagc taacggggac gttacggatt cgacaggcac agtcgagctt
     5461 gaattgcgtt tcgtaggtta cgtctacgta aaaacgttac agttaaatat aactgctaaa
     5521 aacgaaaaca actcttacgc tttagctgcc taaaaacagt tagcgtagat cctctcggca
     5581 tcgcccatgt gctcgagtaa gggtcctaac tttagtggga tacgtttcaa ctttccgtct
     5641 gtaagttgaa aaagagaaca tcagactagc gatacagaat gcctgtcact cggcaagctg
     5701 taaagtgaat ccttaaatga gttgactatg aacgtagatt tttaagtggc gatgtgtttg
     5761 gacgcgggtt cgactcccgc cgtctccata tatgtatggt tgagacagat aaacatgtat
     5821 gaaaaacgtt gttaaatcaa cgttttttta ttttggtcaa ggagagaaaa gtagagaaat
     5881 acaaaatttt ttgcattgat tttgcattca aaaaaacatt caaacattta gaaatcccca
     5941 atacgtattc aggaaaatct attttaaggt ttacaaaaag aacttttgtt cgtacactct
     6001 ttttgaggtg aacttttatg ctgatggaag gaaaaacaca attatggttt aagcatgttc
     6061 cttcaaataa gtttttttaa ttattatttg tcaaattaag cgagacaaaa ctttccattt
     6121 acgtaactca aaaaaacttc taatggtgtt tggtaat
//
LOCUS       DASJSZ010000123         6146 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.129, whole genome shotgun sequence.
ACCESSION   DASJSZ010000123 DASJSZ010000000
VERSION     DASJSZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.129"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            47..1204
                     /locus_tag="U4B03_002609"
     CDS             47..1204
                     /locus_tag="U4B03_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069411.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1230..1670)
                     /locus_tag="U4B03_002610"
     CDS             complement(1230..1670)
                     /locus_tag="U4B03_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN2069412.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1830..2336
                     /locus_tag="U4B03_002611"
     CDS             1830..2336
                     /locus_tag="U4B03_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2069413.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2504..4417
                     /locus_tag="U4B03_002612"
     CDS             2504..4417
                     /locus_tag="U4B03_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN2069414.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4422..5423
                     /locus_tag="U4B03_002613"
     CDS             4422..5423
                     /locus_tag="U4B03_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2069415.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5625..5831)
                     /locus_tag="U4B03_002614"
     CDS             complement(5625..5831)
                     /locus_tag="U4B03_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEN2069416.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2277 a          938 c         1213 g         1718 t
ORIGIN      
        1 gtacttatca atccttagaa aatcttagtc aaaaaattat ataagtttgc gtacgattca
       61 aagaaaatta agagaactta atactatttt gaaaaactat catttatctt gcagcttgaa
      121 aaaaggtaat ccgatagaag gagaagaata ccgtatccga tacttttttc acttgatgta
      181 ttggcagtca ttcgacgaaa atcattctga tatattatca agtataaaaa ataaacgtca
      241 actattagaa aaatttgcga aacatgcgcc atatacgcgt agtatagata gaaagaaatg
      301 gatcagccta ctaagaattt cgttgcaacg aatgaaacaa ggccactcga ttacgtatgt
      361 cccagaagag atgaacaaat ttgtgcatcc attgataaca aaagaagagt ttacgcatta
      421 catactagag cctttttttg aagcaaactt ctggtcgatg aatcacatca ctccaacaga
      481 gcaagcttac ctttatttta tgtttggtgt aatgaacacc tatttggaag aagatttgtt
      541 ggatactcca gataaagtac aagaaagttg gggctggatt gaatcgtcta ttgtagaaaa
      601 ttttgaaaaa aacttcaaga tttgttttac aacttcagaa aaaaattatc tatatgtcaa
      661 tttggcaatg atccatttgt attcaagggt ttttgggaca aaaaagaagg tggatgcttt
      721 tggaaaatca gcagaagaca gagattttca gcatatattt ccagtgatgt atccaatcat
      781 gcaacagttt tttagtgggg tagctcgcag aattccagag cttaatactt attatgaaaa
      841 gaacagacgg ttgatttttc aatactgtat gttagtcagg gatattttta taaaacacga
      901 acaacctgtc atattctata ttcaatcaaa gtatggtaaa acacaggaat tatgggcaaa
      961 gaaaaggata ttatctatga ctgaacgccc aataaaatat agtgcatccg ctgatcagtt
     1021 acctgatatt gtcattagcg attatcctat cgacaaaaag gcatatgatc aaacgaatac
     1081 acagatattt tactggaatg gacaacctac aaaaaatgat tgggcgcgtc tgaaagctga
     1141 aatcataaaa atcaggaatg agaaaaatag atccatattg gaaaaattgg atttcgttaa
     1201 ttaagaaaag cagattgttt atgccaaaat taagacattg caaaaagatt attttcttta
     1261 aaatcgacaa gatgaccaga aagcaagagt tcgttgagct cttgctttct tttagtttta
     1321 gaaaggaaca tcctgatttt atcttcgcta aatccagtca aaagttttgc agattggtcg
     1381 tagataatcg gttttttcaa taaagaaggg gatgtacgaa tctcaatata tagttcactc
     1441 aaactgagtt catctattct gtgaacaaga tttttataac taatagaatg agtagagagc
     1501 atttcggtca cgccgttgtt tgtcaatttt agtaagtgga taaattcatt tttggttagt
     1561 ggttgacgaa ttaagttctg ttcgataaac ggtatatcat ggacgataag ccaacgtttt
     1621 gcgcgtcttg aagacgagca gccagaacct gtatacaatt taatcatcat acattcctcc
     1681 aatagctaag tatttatgaa ataataacat gaagaaatag tcgaaaagtg ccatttagag
     1741 gtgtaaaata agaaaatgtt gtcacgtata atggaactag ctagataaga aatactatta
     1801 aatcaagaaa aaagaaagaa ggcacaagta tgtcaaacac agccgacgca atagtagtga
     1861 tcgatttaca aaaaggggta tgttttgatt cttcgccgat acacgaatta gaaacagtga
     1921 cagcattagt gaataaaaga atcaaaacat acgaaacgat tgggaaacca gttattttcg
     1981 ttcaacacga agatgctcaa ttagtgccac aaacaaaacc atgggagcta ttgtcggaat
     2041 tatctattcc tgagcacagt tattttgtga gaaaaaccca tgcgaattca ttttataaga
     2101 ctaatttgag tgttttatta actgaattgg actgtcattc gatcgaatta tgtggggcac
     2161 aagcagagta ttgtatagat acgactgtga agtttgctca tggattagga taccagctta
     2221 tgatgtgtag aggagcaacg acaacttatg ataatccttt tatgaaagca aaggaaacaa
     2281 tcgcttttta cgagaaaatt tgggataaac gatttctgac atttttggag caataagttg
     2341 tttcttcgtt taaaaaaagc agaatggata ctggtttatc cattctgctt tattacgtaa
     2401 aatgagaaaa taatcctgtt ctatttttat gggataaacc atttattttt tgtataaaaa
     2461 atgtttgcgt ttacatataa ttctcctaga aacataaaaa ggagtgaata gcttgagaac
     2521 aaaacaagta ttaaagggag cgttggtcat cttatttgct gtaagtacaa gcgtcccgca
     2581 atggacagta tatggagaga gcatttcttc cgaatcggaa tctcgggagg tagaaaccac
     2641 agaaactact gatgctggaa atgaaacgaa atcgagtact tcacaaacag aaacaaatac
     2701 tgaaacaacg gttgagacat ctgttactag cacagatcaa gaaacgaccg ctaccacaga
     2761 taaccaacaa acagaagaag caacagctac aaaaatgtca aacgacgaat cctcttctgc
     2821 taaagtaagt gacaaagaga atgggacaga aaatgatgat atcggtaaag gtgaaaattt
     2881 caatggtaaa gaatatttga attacttcgg taaacagatg acagaaatac ttaatggacc
     2941 aacagaagac atcaagtcaa aattaataga gttattagtc actgagcttg ctgttaatga
     3001 ttatggtgta gataaagaga ttatcaaaca tctgattgct acttatctat tgtacgatat
     3061 cgcttttgca aaaaacagtg tgttgatgtc gatgcttgga ggtacagatg atgggacaac
     3121 gcctacagaa aacacaggct tctatccaaa tacaaaacaa cttcatcaaa ctgtactaaa
     3181 caaacaagat ggcaaaatga tggatgtata tgcatattat gtcgatcaag gttcagataa
     3241 aacagtcatg atccatggcg gatttcgagg gaattggaat aacgggatcg ttacagaaga
     3301 atacaatgat ttttataagg ccgggtataa tttattattt gttgattcca gagcgactgg
     3361 taatagcgga ggcgattatg tgacctatgg acaatatgag tcagatgacg tcctttactg
     3421 gataaatcaa gaagttcgag agcggccatc tcaaaagatt ttactttatg gtggttcgat
     3481 gggagcagct acgatgatgt ctgtattagc gaaagatatt cctgtcaatg tcaaaggaat
     3541 tattgaaaat tgcggatttg catcaattga tgaacaacta cgttttacgt acagccagac
     3601 agtagccccc gcactaccgg atgccataaa aaatcagtta gacatcattg gggatcaaga
     3661 gcatgaagat ttatttatgg gccttttgaa acaatactat tttgatcagg agatgcattt
     3721 ggatacaaaa gctgctctac ctacgattgg aatgtcagat tcattgccta aattgattat
     3781 ccatggaaca gcagatgatg tagtacctgt gtctaacgcg caaaaattat atgagttatc
     3841 aggagggtat aaagatcttt tattagtaga aggagcaggg catggaaagg cacaggaagt
     3901 tgaccatgct gcctacacga aacatgtaac ggactttttg aaagtagtgt tccatgatca
     3961 gataaacgta aagtatgtgg atgaaaacaa taaaagtcta ctcaaagacc aggacgaaat
     4021 ccagctttac ggagcttatg gagaaaatta tatgacagag caaaagacat tcgaaggata
     4081 cgaattagca aatgttgaag gaccgactga agggattttt aatgagacga taccaactat
     4141 tattttctat tacaaaaaaa tcccagtagc tccaccaaaa aagcaagatc cgacagagaa
     4201 tgcagacaat aagggaaaga atgaacctcg agaggatcaa tcaagtgatg atacgcaacc
     4261 gattcagatg acagtgaaag tacaaaaaaa gacagatgca aaaaaaagaa tgttgccgaa
     4321 aactggagaa aagaaacatt cgcttctcat ttattcaggt ggatttttat ctgttagcac
     4381 gattatcaca tggatttggc gaagaaagag agggtaaatg aatgaaatgg ttcaagcgaa
     4441 tagtgattgc agtaccagtt atcatcgtag ttattttagt cactgtattt ttgattaatc
     4501 aaatcacacc aaagccgatt agcttgcttg ttagaaaaca atttgacaca agtgaaaaag
     4561 aacaagtata tgcccgacca gatgatttcc agcaaaaaat cgaacaaatc gatataaaaa
     4621 aagaaatatc ctatccttca aagtacaaaa ataataatat ggatatctac acaccgaaag
     4681 tggaaaagaa aaaattaccg attcttttct ggatgcatgg aggcgcatat gtaggagggg
     4741 ataaaaatga ttgtcgtgat tatttggaat atctatgttc agacacgcag caaatcatcg
     4801 taaatataga ttatgaacga tcaccggaag caaaacatcc tacaccagtt atccaattga
     4861 acgaagccat tcaagcagct aaaaaagaga taaaagatca agcagattgg tcgaatattt
     4921 cgattggtgg aaattcggca ggtgcacaaa ttagtggaga atatttgtta gcacttcaaa
     4981 atgaagaaat taaaaaagct gatcatcttg atccgacttt ggagaacaag cagattacga
     5041 agtttatttc tttgtctggt ctgttggatc cttctgcact tactcaagta agtgataaaa
     5101 tctctagctt tctttatgaa aaatgtggat gggcttactt tgataataag gactttgaac
     5161 aatcagagaa ggtcaaaagt ttagccttgg tcagacatgc ggatcgttgg acgcaaaaca
     5221 cattcttaac ggatggaaat acaaatacgt ttacgaaaca aatggaagaa gtaacttctg
     5281 tatttgagaa agcgggggtg aaagtcacaa aagtagatta tgcggagaaa aatgctgttt
     5341 tgaaccatga atatcagttt gatttcagca ataaacaagc gcaagaaaca tatcaaaagt
     5401 tagtaatgtt tttcaaggag taaagtctta tgatgcaaaa gaggttgtaa tctgtcactt
     5461 agctccaagt cacaaggagc aatttcaata aacaagcttt tcatggttta tgaatattgt
     5521 aacttgtgac tgaggagtta gagatggcaa ggggcacctt ttattcgaaa tttgggaata
     5581 tcacacgatt attgtcacga tctcttttac gtaaatttta taagtcagat ttgttgtttt
     5641 gatcgtttaa aaaaagcaac aaatccgaag cctgcggagg caagaaggga caaaaagccg
     5701 agtggtaagc aaaagaatgc tggttccact aatattccgt tgttattgat ataaggctgg
     5761 gaacaaagat gaatagcaaa taaaatcaat gcactagcga aaaaaagcaa agaaatatgg
     5821 atagctttca taagcagcac ctcctttttt ataattgtat gataaataga ttaagaatct
     5881 ataaatgtga caagtttgtt aggatgtgaa taaaatgatc atctagcgaa cttgaaacaa
     5941 agatggctaa tgataagcaa ttaatgattc agcaatcaga taggtacaaa gtttaccatc
     6001 atacgtctag accaataata gaaaatatgg gaatataata gaaaagattt aggatacccc
     6061 caaaagaatt tccccaaaaa ttcgatgtat cctaaacttt tttattttac ataacaatca
     6121 tatcaactgt gaaataaaat tacaaa
//
LOCUS       DASJSZ010000124         6076 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.130, whole genome shotgun sequence.
ACCESSION   DASJSZ010000124 DASJSZ010000000
VERSION     DASJSZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.130"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(254..541)
                     /locus_tag="U4B03_002615"
     CDS             complement(254..541)
                     /locus_tag="U4B03_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2069417.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="U4B03_002616"
     CDS             complement(561..785)
                     /locus_tag="U4B03_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEN2069418.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="U4B03_002617"
     CDS             complement(1240..1410)
                     /locus_tag="U4B03_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069419.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="U4B03_002618"
     CDS             complement(1425..1958)
                     /locus_tag="U4B03_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN2069420.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="U4B03_002619"
     CDS             complement(1979..2923)
                     /locus_tag="U4B03_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEN2069421.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="U4B03_002620"
     CDS             3560..3646
                     /locus_tag="U4B03_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN2069422.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="U4B03_002621"
     CDS             complement(3767..4456)
                     /locus_tag="U4B03_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069423.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4701..5615)
                     /locus_tag="U4B03_002622"
     CDS             complement(4701..5615)
                     /locus_tag="U4B03_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN2069424.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="U4B03_002623"
     CDS             complement(5597..5977)
                     /locus_tag="U4B03_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN2069425.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1610 a         1121 c         1023 g         2322 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagctcac taaagg
//
LOCUS       DASJSZ010000125         6019 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.131, whole genome shotgun sequence.
ACCESSION   DASJSZ010000125 DASJSZ010000000
VERSION     DASJSZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.131"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            253..507
                     /locus_tag="U4B03_002624"
     CDS             253..507
                     /locus_tag="U4B03_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEN2069426.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(596..1642)
                     /gene="holA"
                     /locus_tag="U4B03_002625"
     CDS             complement(596..1642)
                     /gene="holA"
                     /locus_tag="U4B03_002625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN2069427.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(1680..3953)
                     /locus_tag="U4B03_002626"
     CDS             complement(1680..3953)
                     /locus_tag="U4B03_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEN2069428.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(3946..4485)
                     /locus_tag="U4B03_002627"
     CDS             complement(3946..4485)
                     /locus_tag="U4B03_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEN2069429.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(4621..4839)
                     /locus_tag="U4B03_002628"
     CDS             complement(4621..4839)
                     /locus_tag="U4B03_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEN2069430.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(4836..5081)
                     /locus_tag="U4B03_002629"
     CDS             complement(4836..5081)
                     /locus_tag="U4B03_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2069431.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(5197..5925)
                     /locus_tag="U4B03_002630"
     CDS             complement(5197..5925)
                     /locus_tag="U4B03_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN2069432.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         1860 a         1229 c          979 g         1951 t
ORIGIN      
        1 attcaaactt tgtttaaata gataaataaa aatctagttt ggtttgacgc tattgcctcc
       61 cacaatagcg tctttttgat ttttttcagc ttgtactatc cgtttttact gaaaaaaaat
      121 cctaataaaa gactgttaag gaaaaaaact tgtcatgttc atattgcaaa gcacgctgcc
      181 tttaggtata ataatgaatg ttgaattatg cggtaacacg cgtaagaact taagttatcg
      241 gaggtgaaac taatgccaaa cattgaatct gcaatcaaac gtgttcgtac aagcgaaaac
      301 gctaatgtga aaaattcatc tcaaacaagc gctatgcgta ctgcaatcaa aaaatttgaa
      361 gatgctgtag cttcaggtgc tgacaacgta gatgcgttat ataaagaagc tgttaaagct
      421 attgatatgg ctgaatcaaa aggtctaatc cacaaaaaca aagctaaccg cgacaaatct
      481 cgtttaagca aaaaaatcgc taaataaaag aatcggcttc ggccgatttt tttatttgtc
      541 aaaaaaagga caaaaacaaa cgaccttccc gcatgctttg ccctcgtctt tcttcttatc
      601 gatttcttaa caactgccct gtgcgcaaaa cgaatagttg aaaacttaat tctttttcga
      661 tttgtcccgt tttgatttga taatccatct caactaattg atcaaacaac tgtaccagca
      721 aatgttcatc gaattttttt acttcctgca tggcaagttt tacacgatat ggatggattt
      781 tcaatgtttc agcaatattt gcctgctgat aaccaatctt tacaagaaat tttgtctgta
      841 aaaataaacg taattgagaa agcaaaatcg cattaatttt gattgtttct tccccttgca
      901 taactaaatc ttcgtataaa cgcaaagctt gttccgtctt cccttttaaa atatattggg
      961 tcatgtcaaa aatattatgt tctaacgttt taggaactaa ttcttctact tctcttaccg
     1021 tgatttgctg ttgattttgt ccataaagga ccaatttctg taactctctc atcattttgg
     1081 ataaatctaa gtcggtcaac ctcaagaata aatcaattgc acttcgttcc attttgattg
     1141 aggaattctc taaggtattc atcagatatt gacgtacttc tttttcattc aactgttgga
     1201 cattgatgat aattgctgtt tttttcaatt gtttcgttac ttttttccgt tcatccaact
     1261 tttcatatgg cgcaaagaat actaatacag tcgattctaa tggttctttc aaataagccg
     1321 tcaataaatc agtattttgt tcgattccat tgtttacttt ttcacctgtt aaaaaataag
     1381 gattctctac gaatactaac cgttgatctc cgaaaaacgg caacgtctct gcttcagcta
     1441 ctacctcatc taaacttgca ttttccatgt caaaacttaa aaaatttaag tcatcttcgc
     1501 cctctaattg gatttttttg acaatttctg tccgaacttg atcttggaga aacttttctg
     1561 ttcctaaaat caagtaacaa gatgcaagtt tttctttacg tattttttgt aactctgctt
     1621 gtatactcaa attactcacc actttcaaaa attatcctac catgagcaga tgagaattac
     1681 tagtctatca ttgtttccac tagcccatga ggatgaaaaa ctggatgcca gcgataggtg
     1741 atcattcctt gttgatccgt tcgccatgtt tttatctgat tttcttctag tgtctccaat
     1801 acttcgctgt gcgggtgacc ataacgatta tttacgccgc aggatataat cccatgtttt
     1861 gcttcaatat gggaaagaaa atctgtgtta cttgacgtac ggctaccgtg atgacctaat
     1921 ttgactacat cggctttcag attaggataa atcaaaagca attgtttttc cattgcttga
     1981 ttcaaatctc ctaaaaataa aaagcgccta ttgttatatt ccatccatag accaatcgaa
     2041 tcttcatttc ttccctctcc cttttcaaaa ggagtcaata cataaagatc ataataaccg
     2101 ctgatcctgt ccccatgttt gatttcaatg atctcagtac catttttttg agcttcagat
     2161 aaatgccttt gtaatggctc atttcgtcca gcgccccaac cgatataaat ttttttgata
     2221 gtaaaatgct gagataacgt gggtaattct cccacatgat ccgtatcgtc gtgtgtcaag
     2281 atgagtttat cgattacact tatcccctga ccttttagat atggaacgat atttctttca
     2341 gcaggaggac gtatcatacc ttgcgcccac ttttctcttt caaagtgtaa ctgacctcca
     2401 gtatcgatca aaatcgtttc ttttcgaaaa ggactttgca aaaagatact gtctccttga
     2461 ccgacatcga taaaagcgat cgttgaagaa aagcgtaaaa atggcaaagt caaaaaaata
     2521 aaagaaaaaa gaccaacata aaacaagtgt ttcacaggtt gttctttcaa cctatccata
     2581 caaatcagta atccgaaaat atacatccca gatagcacaa ggggcaaacg tcctataatg
     2641 agagacgacc ctgaagaaaa atcgattatt cgctcaaata agattaatcc ctcttctatc
     2701 agcaaaagta aagtttccgg cagccagcga ttgaagagtg tgagaaataa cacaccagga
     2761 agaaaaagaa acttgaatac cggtgcaatc agtaatgtaa aaagactacc taaaataggc
     2821 cactcataaa aatgccatac aatcaacggc atcgtgatca tagtaaacag gacgtgagag
     2881 gccgtttttt tcaaccaatc tccatcaaaa gaaatcagga taaacaaaaa actcatgaaa
     2941 aaactaagct gtccacccgc atgaaaaaga aatcgaggat caaatagtaa aagaagccaa
     3001 aagacaactg caaataagtc catggtggaa taatgcttat tcagcaattt gactagcaat
     3061 cgcaataaaa acagcaaact agcacgcaaa acagaaatag aacttcctgc aatagaaaca
     3121 taacagatag ttagtagact caacggaata atactgacgt gtagtggcca tccgatacgt
     3181 cgaaacaaat aatacagcca ccctaaataa aattggatgt gtagacccga tagactgaac
     3241 agatgcagca aaccactctc cctaaaaatt tcttctggtt ctctgaatcc tgcatcttta
     3301 tagccccata ataaagaatt aatatataca cttgtttttt gcggaaattc agattggaca
     3361 aaggagataa aagatcctct ttttctctga agccagtttc gccagtgatt ttgcatggtg
     3421 atctgttcaa tcttttgaat ttggaaccgt ccagaaattc tatggttcct gaagtaagct
     3481 tcttggtcaa aattataaag attccgcttt tttgctggag agatgaactc gccttcagca
     3541 gtcaattcta ttccctttcc tcggaagtta agccatgttt tcaattctgc ttctgtgttt
     3601 acttgataag aaagctggag cttttgttta ttctctgata accttccttc cattgtcaaa
     3661 aaattaccat ttgcacgaat cgtatctgta aatacagtaa tttgcgcaga tgttgttttt
     3721 tcctctaaaa atgaatactc ttgtagccta taagaaatgg ataccgttat gctacacaaa
     3781 atacacaagt acgcgataaa acgtccttct tttagcgaaa accataaaaa aattccggca
     3841 agaagcaaac gataacccca attgtcagtg taatacaaca aacagataat catcgacgta
     3901 attgccggaa aaatcaaccg atttcgaaca tgcctttcta aaacgctaga catgttttcc
     3961 tgttaattca ttttgaagtt ttgaaaaatg ctgtgagtct aaagcaactt ggtgtacaga
     4021 tacacctaat tgttcgatca acgcttgggc ataaggatca tttcgatagt catggagata
     4081 attgatccct ttcacaccag cttgcaacaa agcctttgta caggccaaac atggaaaatg
     4141 tgtgacatag atttctgaac cttccgtcgg gataccaagt tttgcacact gtaggagtgc
     4201 attcatctct gcatggatcg ttcgcaagca gtgtccatcc acgacgtaac acccttcatc
     4261 tatgcagtgg acatctccac tgactgctcc attatagccg ccagcaatga tccttttttc
     4321 tcgaacgagt gtcgctccta cttctaaacg tgtacacgta ctacgtaaag ataacagtac
     4381 tgcctgagcc ataaagtact ggtcccaagg aatgcgttca ttatgtgact tatcagtaac
     4441 tttttcttta ttcgtatcgc atgcttttga agatttttct aacatgtttt atattcctcc
     4501 gttttttcaa gttgattaaa aagtactcat tgcttaaaca aaatattctt taaatataac
     4561 gaatcatcct gtctgacaaa agatcctacc cttcactgcc gaaatgaagt ccgtcatcca
     4621 ttagcccatt ataaaaattc ttcgaatcca tttgttcaag aaattccgta taaaaatcga
     4681 tacttcacaa tcatggcgtt ttgctacttc tgtgagaaca gagatatatt ctttgatcaa
     4741 cttgttattc cgatattttt gttttgtctc atcgactgct ggtggcgtga taaagaaagc
     4801 atgatcagaa ctatagtctt ttttcagctg aactatcatc cactgcagat tttttggtac
     4861 gtttctttag aaagttgttt atgctctgct agatcattcg tcccaatgag aataaatact
     4921 ttatctgctg tatagctaag tacatctttg gcaaagcgtt ctcgtagagc ttgactgtta
     4981 ttgccgctga cagccgtatt cataatatga atcccagata ttttattttg gagatagaca
     5041 ttcagcattg gtttttttaa tgtttctttg cgaacaatca agctatttcc tatccaaaaa
     5101 tactacctcc tcgtcctgcc aagctcttag aattggatta ttcttagtat ccattagcgt
     5161 ccatttattg ctcgtgccag tcgattttga atcattctat ttaattgtaa cgaagttttt
     5221 tagtttttca actgtctttt caccgatacc agaaatctcc tgcaggtctt ccactttctg
     5281 gaaggatccg ttttcttctc taaatcgaat gatttcttgc gctttgactg atccgatccc
     5341 aggaatctgc tgaagttctg atggagtggc tgtattgata tttattttgg gaggctcgga
     5401 atctgctgta ggctgttttc cttccgagga tgccaactga tcaagatgtt ctggcagctc
     5461 ttcacctaca gcagggatat ataggacttg ctgatcttct atcttcgcag aaaagttgat
     5521 tcgatcgcta tccgcttctt ctaataatcc acctgcacgc tttagtacat catagacccg
     5581 ttcttctgaa gaaaaggaat agataccagg ctccttcaca cttcctttga tatcggtgta
     5641 catgaccttt ggacgagcat cctcttctac atcgtttcct tcagctgatg gatcttctcc
     5701 actggaacta aacgaagaaa gtatttcttt tgcagcagtc tcttctgttt ctctataaaa
     5761 tagcaaattt gataataaaa aaccacttgc gatacaaaag ataagaacta tcatagacat
     5821 ccaaatgatc aatttgggtg aagctagtat tttcttgctg attttttcta tcattgttct
     5881 tttttcaaag aatgaccagt aaaaatcatt cattactcct ttcatttaaa attgaagaaa
     5941 cagtgagctc tggcaaaatc gtcccttcaa catctaatta cgtcaatttt ttcttttgtt
     6001 tttattttta tcgtgaaga
//
LOCUS       DASJSZ010000126         5981 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.132, whole genome shotgun sequence.
ACCESSION   DASJSZ010000126 DASJSZ010000000
VERSION     DASJSZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.132"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..415
                     /locus_tag="U4B03_002631"
     CDS             59..415
                     /locus_tag="U4B03_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2069433.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            405..671
                     /locus_tag="U4B03_002632"
     CDS             405..671
                     /locus_tag="U4B03_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2069434.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            830..1831
                     /locus_tag="U4B03_002633"
     CDS             830..1831
                     /locus_tag="U4B03_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEN2069435.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2201..2815)
                     /locus_tag="U4B03_002634"
                     /pseudo
     CDS             complement(2201..2815)
                     /locus_tag="U4B03_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3055..3300
                     /locus_tag="U4B03_002635"
     CDS             3055..3300
                     /locus_tag="U4B03_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069436.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3398..3619
                     /locus_tag="U4B03_002636"
     CDS             3398..3619
                     /locus_tag="U4B03_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069437.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3941..4621
                     /locus_tag="U4B03_002637"
                     /pseudo
     CDS             3941..4621
                     /locus_tag="U4B03_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            4683..5300
                     /locus_tag="U4B03_002638"
     CDS             4683..5300
                     /locus_tag="U4B03_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069438.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT         2202 a          820 c         1070 g         1889 t
ORIGIN      
        1 aattcgaatt tttatgttat atactataat ataaaatcaa tatatgatat aattgggtat
       61 ggaaaaaata tcttttgaaa catttaaaag acctaatggt catgatgaat ttttagaatg
      121 gttggaaaca ttaccaaaaa aagatagtgc gaagctttta cgtacaatag aagaaacaga
      181 aaagaatggg atgctagtag ctcaacggtt gaaatgggtg aaaaaattag acactaattt
      241 gtacgaatta agaagtaagg taggatcaaa catccaaaga gctatctatt tccatgtggt
      301 caatggaagg tatgtcatta cccatggatt tactaaaaaa acggagaaaa caccaataag
      361 tgaacttaaa catgcaaaag aacttagaga ggagtggttc acaaatgatg attaaaaaat
      421 tgattgaaga aaagcaaaaa gacaaagatt ttgcagaagc atatgaccgt gaaggaagaa
      481 aactcgaaac tgcggttgct ctttatcatg cacgggagga agcaggccta actcaagcgg
      541 agcttgcaca aaaagcacat acaactcaat ctactattgc tcgaattgag agaggggata
      601 acgtatcttt cgataaactt gatgatatcg ctagggcatt aggcaaaaag ctggtagtag
      661 aatttgctta aaagcgagtt gaaaatatac ctgtaatagg ctcctaacta atcaattagt
      721 ttagtgggcc tttttgtact aaatagataa aagttgtcct atagtgtaaa agtcaataaa
      781 ttttatacaa aaattacttt aaagagacat aaagagaaag gtacaataaa tgaaaaccat
      841 aaatctaata aaagtattta tagcatcacc aggtgatata gcagcaaaga ggaatgaagt
      901 agaagaaacc atatatagat ggaatataga aaatacagat aatacaaaag taattttgat
      961 gccaattcga tgggaaacga atgcgattcc tacttatata aataatagct ctgggcaaag
     1021 tgttattaat aaacaaattg tagaaaatag tgatatatta ttagcgattt ttggccataa
     1081 gttgggttct ccagttgata attatagatc gggaactata gcagaaattg attactttta
     1141 taaaaatagc aaaggcgagg taggggtttt tttcttagat gaaccagttc cacaagtttt
     1201 ggctgaagag tatatagaag ttaagaagta cagggactct ttagaagata agggttttta
     1261 tggaacatat agcaaagaca atatcagaag gtttctcaca aaaaaagtga attatttaat
     1321 tgagaaagca caaaataatt tagaatcaaa acgctttgat ttagattcta tttcttacaa
     1381 gacaccttca gtaattaaca aaaaagttac cggaactgat aaagattttg atgacaactt
     1441 agatattttt gatccaatag aatttgacaa tgatgaaaga ttgctgctaa tatatgcgtt
     1501 tatggatgaa aattctatct tttcaactga taattcaact tatagaaaag tagttcaatg
     1561 ggaaagtgat aacaatttaa ggagttactt gagtgataga gtatttgatg tactagaaaa
     1621 attaagtaaa tcgggaattt taaaggaaga atacgattct tcttttgatg agtatccatc
     1681 acgctatatt ttgatttcag aggactataa gaaatttaaa aggtattttt taaaaaatga
     1741 aaaaaagata tctgttatta tggagaaata caaagctcca atacaggctg atccattttc
     1801 caatttggat gaatcagatt taccgtttta aattttaaat agataatttc attttttagt
     1861 agcagaggta taaacttttt taatgtttga attgaaaaga aatgctaata ggtcgacttt
     1921 atgtttctct tgtgcaatcg taaatgcggg ggaccataaa attgatctat tatcgagtgt
     1981 gtataaaata aaattcaaat cttattagtt tacataagat acattatact aagttgtcaa
     2041 tgttaaatgc ctttaaataa ggacgattta tgcatcaatt atatttatgt taactaaatt
     2101 ctttttaaca taaatgtaat caacttagct agatgaatct aaaatgattg tctgtattga
     2161 tgacaaagct aattaataat tacgtgattc aaaaaagaaa tcatacaatt agttggtcga
     2221 tttctttttt tattcggtaa attgtttgcc tagtcacatt gtagtctgtt gcaatctttg
     2281 agatagcaac cccattctca agatcctgaa cgatactcct atagacaagg cgacgttgag
     2341 gatctttggt gtctgcactg tataacttag gacgaccttt atagacgccg tttgctttag
     2401 cgattttaat tccttgtgcc tggcgtcgtt tcgattcagt acgttcttgt tcagcaatca
     2461 tggctgagat ttaaatgatt aagtctttga tgaatttatc taggagagga tttccaattg
     2521 cttcagccat gatcggtaaa ctggtgataa taagtcgcac attcttcttc tttagataat
     2581 tcactgaatc gataatctcg tcataattgc gtccaagtcg atcaatggct tctacaataa
     2641 aaatatcttg ctctctgaca aaatctaaag cttcctggaa aatcggccga tgactaatcg
     2701 tcgctccaga ttgtttttcg ataaaaattt tttcagcgcc aaactttttc atttcttcaa
     2761 tttgacgttg ttcattttga tcgatggatg aaactctaat ataagcaatt ttcaaattta
     2821 ttccctactt ttctataacg tttataatac accctaatca tacgttagaa ctctaaattt
     2881 ttcaatgtta tatttttgtc tatttagggt aaactctaaa tagacaaacg gtttttgaat
     2941 tctaaaagtt aaatagttta taatagaaga aaaagaatgg ggtgacgatt tcttagattg
     3001 aaaaggaaag ataaatatta tagattatta cttttgacag ctgaagcagg atggatggat
     3061 tttccagaag ttgtaaaaga agttcaagag ataggtgcaa ctatttctga tagccgctcc
     3121 tctcctatga actatcagga tgcccatgga attaaaataa ttgatcaaga gagaaatgtc
     3181 agaaaataca caacaataaa tgagtgtgta attaatgaaa atctttgtag ggcaccgata
     3241 acgaaacaca ttaagaatag aagcaaagca aaagacggaa gaacttttac taccttctaa
     3301 gtttttttca tatttgtttg ctatacttag atctaaggaa ttagactttc gctgcccatt
     3361 atttaagttt gaggtagcgt cttgcgaggg atcaataatg gaagaagaaa aaatatttga
     3421 aaaacgctgg gaattggctt cagttgaaca aagagcaagg taccataatc taatgtcctc
     3481 ttaccgtaat attgattgga cttacaaaga aaagaagtac ttgctttggt tatgtcagtt
     3541 agatgtaaat acctttgaaa catttgaagt gattctagat aagattaaaa acagcaatga
     3601 aaaaagagct gatctataat ggttcgctct ttttatttta ttaactcttt ggttactata
     3661 tgtaaaatac agcttcatat tacaataaat ttcaggatac aaatttattt aagcatatat
     3721 aaatataatt gaaataggac ataaatatat gagtatgaga aaggtgattc catgtttaat
     3781 aaatgggcaa accacccaat gaactcgaga ggtagagata atttgaagga gacggacaag
     3841 ttctgggaag aagtatatag taaaaacggt tctgttgcaa agttttccaa aaaatctatt
     3901 ttagtgtaaa attgagaaaa aagacagaga ggacagagta atgaatcatt ttaaaggcaa
     3961 acaattcaaa aaagacgtca ttattgtcgc tgttggttac tacctgcgtt acaatctaag
     4021 ctatcgtgaa gttcaggaat tgttatatga tcgtggaata aatgtttgtc atactacgat
     4081 ttatcgttgg gtataagagt acagcaaagt cctctattat ctttggaaga agaaaaatag
     4141 acaatccttc tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca
     4201 ttatctttat cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa
     4261 acgggatacg caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc
     4321 gaaagcaatt gtgaccgata aagcaccttc tcttggctcc gcctttagaa agttacagag
     4381 tgtgggttta tatactaaga cagagcaccg aactgtgaag tatcttaaca atttaataga
     4441 acaagaccat cgacctatta aacgacgaaa taaattttat caaagtctcc gtacagcctc
     4501 ttccacgatt aagggcatgg agaccattcg aggaatatat aaaaagaacc gaagaaatgg
     4561 aacgctcttc ggcttttcgg tgtctactga aatcaaggta ttaatgggaa taacagccta
     4621 agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca gaaccgagaa
     4681 ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt ttaaagtgga
     4741 aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt atttttactg
     4801 tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa ggtcgtttag
     4861 ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc ctatatgact
     4921 atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa gtaaaagttg
     4981 gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc tatacagtag
     5041 aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga acagttgtga
     5101 aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct attaaaggta
     5161 cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg attctatctt
     5221 tattaattag ttcaataact tttatattct tatttacacg ttatttaata agatgctgcg
     5281 gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt tcatagcaat
     5341 ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg aatttattcc
     5401 gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat aggaaaacgt
     5461 agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa
     5521 actaatttag tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg
     5581 tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt caactctttc
     5641 gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt
     5701 atgatatata tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta
     5761 aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg
     5821 aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt
     5881 tattgattgc aaaattatgt gacaaaaaaa actagtagaa gaaagaataa tggcttgata
     5941 ataaagtact acctattaaa ggtagtactt tatttaattt a
//
LOCUS       DASJSZ010000127         5972 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.133, whole genome shotgun sequence.
ACCESSION   DASJSZ010000127 DASJSZ010000000
VERSION     DASJSZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.133"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..450)
                     /locus_tag="U4B03_002639"
     CDS             complement(<1..450)
                     /locus_tag="U4B03_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2069439.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="U4B03_002640"
     CDS             674..1465
                     /locus_tag="U4B03_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2069440.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="U4B03_002641"
     CDS             1431..2384
                     /locus_tag="U4B03_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEN2069441.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="U4B03_002642"
     CDS             complement(2782..3756)
                     /locus_tag="U4B03_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2069442.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="U4B03_002643"
     CDS             3871..4305
                     /locus_tag="U4B03_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2069443.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5452)
                     /locus_tag="U4B03_002644"
     CDS             complement(4430..5452)
                     /locus_tag="U4B03_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEN2069444.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5844..>5972
                     /locus_tag="U4B03_002645"
     CDS             5844..>5972
                     /locus_tag="U4B03_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HEN2069445.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGL"
BASE COUNT         1876 a         1126 c         1029 g         1941 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaaa ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tg
//
LOCUS       DASJSZ010000128         5779 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.134, whole genome shotgun sequence.
ACCESSION   DASJSZ010000128 DASJSZ010000000
VERSION     DASJSZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.134"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..813
                     /locus_tag="U4B03_002646"
     CDS             <1..813
                     /locus_tag="U4B03_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN2069446.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="U4B03_002647"
     CDS             807..1229
                     /locus_tag="U4B03_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069447.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="U4B03_002648"
     CDS             2363..3331
                     /locus_tag="U4B03_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2069448.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="U4B03_002649"
     CDS             3341..3829
                     /locus_tag="U4B03_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN2069449.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="U4B03_002650"
     CDS             4125..4406
                     /locus_tag="U4B03_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069450.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="U4B03_002651"
     CDS             4408..4824
                     /locus_tag="U4B03_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069451.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5438..>5779
                     /locus_tag="U4B03_002652"
     CDS             5438..>5779
                     /locus_tag="U4B03_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN2069452.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2155 a          983 c          943 g         1698 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcccttggt
     5281 acaggcgtta cttggtagta tacacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DASJSZ010000129         5723 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.135, whole genome shotgun sequence.
ACCESSION   DASJSZ010000129 DASJSZ010000000
VERSION     DASJSZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.135"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(672..962)
                     /locus_tag="U4B03_002653"
     CDS             complement(672..962)
                     /locus_tag="U4B03_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069453.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1364..1600
                     /locus_tag="U4B03_002654"
     CDS             1364..1600
                     /locus_tag="U4B03_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069454.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1607..3559
                     /locus_tag="U4B03_002655"
     CDS             1607..3559
                     /locus_tag="U4B03_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN2069455.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3631..4128)
                     /gene="dfrG"
                     /locus_tag="U4B03_002656"
     CDS             complement(3631..4128)
                     /gene="dfrG"
                     /locus_tag="U4B03_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN2069456.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4455..5147)
                     /locus_tag="U4B03_002657"
     CDS             complement(4455..5147)
                     /locus_tag="U4B03_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN2069457.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            5542..>5723
                     /locus_tag="U4B03_002658"
     CDS             5542..>5723
                     /locus_tag="U4B03_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2069458.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         1863 a          995 c          928 g         1937 t
ORIGIN      
        1 ctttaagcat tacttaaaga taacttaaaa gttatctttt aaatacttaa ttgttaaata
       61 ccttatttga tgcaaaagaa atctacgata gtcaacaaaa aaattttcat tatatccaga
      121 aaaatttttc ttagttaagg acctatggat tgcagtaatg cagaattcgt cgaataaact
      181 gccaaagaca tacaatcata ttgcttttaa aattgacgaa caagatatag attcgtttgt
      241 ctcaaaaatc caaatgttag gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga
      301 ggcttcttca atttactttt acgactatga taatcacctc ttcgagttac ataccggtac
      361 tctccaggaa cgtttaaaat cctacaattc cactacctga gactagtaat gactagtctc
      421 tttttatggc ctctgatccc cccattgagc gaatactaat agggcttcga attgatctgt
      481 tcaatcttta agcaatatcc ctcaaatgag tttggaaagt ttagtgattc atcacaaata
      541 cttaaataca tgtagttata tatagattga ggttcttgtg agcgtatgct cgtgtgggtt
      601 caaatcccat cgtctgcatc catctttcca aaacaagcgg ctaggacatt tttgtctcag
      661 ccgcttgttt tctagtttat tctttcatct tgtcggacct tttttcttgg gtatagactt
      721 ttctcatatt ctaccacaat tttgggtttc cctaacacat gataggatct cacaacaaat
      781 tgctttctat tattataata acctgtaacg actaacttca tcccttcgtc tacttcagct
      841 aggaagttca aactatggga agcaatcaaa caattgaccc catttaaact gaaacggatc
      901 aacggtctgg cactaacttg caaagtacgg atcttatcta ctgttccata ttttttctcc
      961 ataaccttca cctcaatacc attatacgaa catacattcc ctttgtaaag aagaaactct
     1021 gttttatctt tttgactttt tgtctattac ctttcttaaa atcatattgg cacttcctgc
     1081 gaagatgaag gacatgtact aaaaaaaaga ggcagcagta tagatttaag tagttcagtt
     1141 ttggagtaca aaaagtgaaa atgtaaacct aaatgagtat caaattgcaa ctgaatttga
     1201 tactcgtttt ttattgttaa atcaacgttt gttgttaatt tacaccacaa aatgtaagtt
     1261 aaaatgtata taaaaatgcg aacgtgaaat aattattgta ggaatgcaaa ttggtgtgtt
     1321 aaaatggtta caatttacta agcaaaaagg ggtgcatttt cctatgcaat ttcttgagcc
     1381 aaaaaataag aacgctaagt ctgtggattg ggaaatatca gagcaagtaa gggtgatagt
     1441 taagcaatac gctgaatatg cagagcgtac tgaaagcgaa gcagtggatg aattcttatt
     1501 gaacatttta gacgataaga agtttattga atggattgcc aacaagcgaa gtaataagcg
     1561 gattgttgaa aaaatgggta tcaaagatag agtgggttga tagacaatga ccaagcttaa
     1621 aagattagcc acgaaggaag atgaaattgt agatgttaaa attccgattt caaacgaaga
     1681 attaaaagat agggctaaac aatatgattt attaactcca aaacgatttg caacaaggta
     1741 taacaaaatg ttgttcttgc caacgtcttt taaatggaac ggttctgaat atcccattca
     1801 atataattac tgcatcaatc cgttttgttg taattttggg aaagaacagc acaaattcaa
     1861 agatgtaaaa gggaaaccaa gtcgatataa aatgactggt agttccaaag ataaaggtca
     1921 taaaggtatg tactgcaatg acaatcctat tggcagaggt gtatcgcaaa actgtacagt
     1981 aaccccttta tctaattggt ctgttgttga agaaattaaa cgccttatag aaatcaatag
     2041 catccaagat gttgagccag attatcaatt tcataaagaa ggctgctcgg aagaagaatc
     2101 aaccccattc aatgagccga agcaatttta caaaaggggg aaaagtaggg gcaaatctca
     2161 acgctatcaa tgtaaggcgt gtaagaagtt cactaatgtt ctaccaaaaa gagaagaaac
     2221 cactacttat caccaacaaa agaacaccat tcttccaatg ttcgctaaga tggttgttgg
     2281 aagggtttca gtcagtcgta cttgtgatat tttaggaatt ggagttggaa cctactatca
     2341 taagctggag tggctgtata ggcgttgttt ggagttttta gaacgatatg agacacaacc
     2401 ccttcaaaca aagaaattta acgagatgtg gcttaatacg gacaagatgc actactacct
     2461 aaataacgtc cgtaagaaag gacaaggttc aaagaaatat acggggtttg aagaccttaa
     2521 tatgcaaacg tatatagttg tatctgctga ggtcttatca agatatgtgt tccgttctga
     2581 tgttgcctat gattggaata tttcaatgga tgaattaaat gaagatacaa gaaaattcaa
     2641 agaagaccat ctcaatactt ttagtcgtaa aaatgatagg ttagattggt cttattatcc
     2701 acaagaacca tccgctaacg atagcgaaaa taggaatgct tatcttcacg aattagggaa
     2761 aattactaat aggagtagat ttgtcgatgg tttaaatgtt gatgctcctt atactacaac
     2821 tgctcactat tggttaatta aacaaatggt taatgccgac gaatggcgaa tgattagtga
     2881 tgatgacttc tctattagaa acgcctttta tagagtattc acgaaagaat taaggttgag
     2941 cgatgcacat cattttattt gccaagttaa caagacgaaa agcagaaaac aatgcttaaa
     3001 agaattcgga caagcaaaag cagaactgct cgattgggga gatatcagag gattcaaaac
     3061 aaagtttcta cggactttgg catctcatta tctaactgaa ttactaacaa gtcatcaatt
     3121 ccacgaagag gctattagta aagatggaga aagatatcgc aaatatgccg ataaccctat
     3181 caaacatcca ttagccacta aggataaagg attttattca gttgattgta gaacggattt
     3241 atctgctctg gaaccaaacg aaatcgcaaa gatgctatta aatgttaatg accattcgac
     3301 caatagcttt attcaacaga taagacgtta tatatcatct cttgaaagac cattaacaac
     3361 agctcgtggt gataagaaga gctatattta cgctaacttc aatccgaagt atgctcaatt
     3421 tgcgattaca attctaagaa cttactacaa cttttgtcgc ccttttaaat cagcggataa
     3481 aaaggttttg acacctgctc aaagattagg gattacagat aaacaatttg attggaaaga
     3541 tataatttat ttcaaatgac aaaaagagct tggagtaatc ccaagctctt gtgctttatc
     3601 tttactgttc ttcttcaatt aacgcacccg ttaactcaat aagttttttc tttcatatac
     3661 atgaaaatag tagttatacg gatttttatc attctttatc cctttttggg caaatacctc
     3721 attccattcc tcataattca cttctggaaa aaaagtatct ccttcgaatt catgatgtat
     3781 ttttgtgatg tacattttct caacataagg gaaaaacaaa ttataaatct gttctcctcc
     3841 gaaaataaaa atttcttctt cgtttttaca taactcaaaa acatcttcta ttgaatgaac
     3901 aatttcacaa ccattaaagg taaacccctt atctctcgtc agaataatat ttcttctgtc
     3961 aggtaaggct cttccgattg attcaaggtt cttcctacct aatattatcg gatgtccctt
     4021 tgtagtattt ttaacatatt cccagtcctt gggaatcctc caaggaatgt cattctcttt
     4081 gccaatcact ctattcttat ccatcgcagc aatcaaagaa actttcattt ataacacagc
     4141 ctttttaact aatttaccat ctaatttctt atttctatca aaaaaccttt tctcattatc
     4201 ctgcccgtta gttgaataag caattttttc tataagttat attatcataa taccacaaaa
     4261 tcacaaattt tcattcaatt atcattttca attgcatttt ataaatcatt ttgtatgtaa
     4321 tttggtatgt aagttaaaat gaaaaaagta gtccattttg aagtacattt tgtcattttg
     4381 tactccaaaa ctgaactact tagtatagat tactgttgct tctttactaa tttaggttta
     4441 cttgttaaga aagattattt acgcaaatgt tcttcaacaa tttgctgaga tttatcagct
     4501 gtatcttcca cttcatcttt tgctttttct aacttttgaa tatattgttt ccagcttgag
     4561 ttttcagcag ttgcttcgtc taatttcttt tgtgtatctg ttaactgagt ttctacattt
     4621 gttttttctt gtgtcagtgt tgcgatttgt tgattcaaac catttacagt attttgtaat
     4681 tctgaaagtt ttgcttgtgc ttcttgtagt cttgctgtcc aatctgcatt cagatgattg
     4741 atttcatcat ttttttggtt gatctcatct agtttgacat tgatttcatt ttgtttgctt
     4801 tgtagttgtg catccttgct gactaattga ctttctaagt tttgtttttc tgctgccgat
     4861 tgattttttt ggttttctaa ttcatttact tttgtttggt attcttggac atctgccttt
     4921 gaatcttgca atgtctgttc tttttgtgct agtcgagcgg taagatctgt aatcgcttgc
     4981 tctttatctg tcaatgtatg atcaagctgt gtcagattct cattgattgt atcaatatct
     5041 gtctctccac cccacaaaat aatttcattt gcgtaccgcg tgcctgtgta gccaattaga
     5101 catagaaatg caacgacaat cccaccagta ataaatttct ttttcatttg catagctcct
     5161 taatccaata cttttacatg ctaaaagtaa gtgatctcaa acacaatgtc aagcgttttt
     5221 aaattacata tgtaatttta atataaatac tatcaaatac tcatttaatc ctcctttttt
     5281 atctatttaa aaaatctttt ttatagagaa aaatgcaggt taatactttc ttaaataaat
     5341 atttatggtg aacaaaaaca aaaaagcaag ttttattaaa cgtaaccctt ttcatttcat
     5401 agattactgt atttattaca aaaaacgcgg atttgtcata aattgtcatg cttcttttac
     5461 ctatgaaata ttttcacgtg ttaaagtatt cctcgttgaa cacaacaaat aaaacttttt
     5521 aacgaataaa ggagattttt catgacatca cttaaaactt tactttttgg aacaactttg
     5581 gctgccggcg ctgcattctt catgggaacg actgctcatg cggacgaagc ttataccgtt
     5641 caatcaggtg atactctatc aacgatttct caaaaatacg ttggtgataa ctccttgatc
     5701 aatgccattg cagaatcaaa ttc
//
LOCUS       DASJSZ010000130         5313 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.136, whole genome shotgun sequence.
ACCESSION   DASJSZ010000130 DASJSZ010000000
VERSION     DASJSZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.136"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(35..310)
                     /locus_tag="U4B03_002659"
     CDS             complement(35..310)
                     /locus_tag="U4B03_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEN2069459.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(282..1235)
                     /locus_tag="U4B03_002660"
     CDS             complement(282..1235)
                     /locus_tag="U4B03_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2069460.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1847..3340
                     /locus_tag="U4B03_002661"
     CDS             1847..3340
                     /locus_tag="U4B03_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN2069461.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3475..3771
                     /locus_tag="U4B03_002662"
     CDS             3475..3771
                     /locus_tag="U4B03_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN2069462.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3872..4723
                     /locus_tag="U4B03_002663"
     CDS             3872..4723
                     /locus_tag="U4B03_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069463.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4744..5027
                     /locus_tag="U4B03_002664"
                     /pseudo
     CDS             4744..5027
                     /locus_tag="U4B03_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1814 a          824 c          999 g         1676 t
ORIGIN      
        1 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
       61 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
      121 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
      181 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
      241 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
      301 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
      361 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
      421 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
      481 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
      541 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
      601 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
      661 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
      721 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
      781 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
      841 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
      901 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
      961 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1021 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     1081 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     1141 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     1201 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     1261 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     1321 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     1381 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     1441 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     1501 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     1561 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     1621 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     1681 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     1741 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     1801 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     1861 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     1921 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     1981 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     2041 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     2101 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     2161 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     2221 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     2281 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     2341 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     2401 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     2461 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     2521 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     2581 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     2641 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     2701 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     2761 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     2821 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     2881 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     2941 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3001 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     3061 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     3121 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     3181 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     3241 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     3301 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     3361 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     3421 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     3481 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     3541 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     3601 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     3661 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     3721 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     3781 tttacttaaa aaattggttc attgagcaac ctatcgctgc taaagtagca aaggaaaacg
     3841 ttaaaggaaa gaatttaggg aagaaatcga aatgaaatcg gacgactgga ttgaacacac
     3901 ctggcttcct ctgaaaagaa atagcaaacg aaagaaattt tgggtattgc aattatggcc
     3961 attaggaaaa ctttgtagtt acggggcacg acttattctt gatttaaccg tttactcgat
     4021 tggagtattt ggaacggctt attacttgat aaatgatccc aataggattg cagcatcaga
     4081 catttttaca gtgctaatga cgttgatttt tgtcatgcta ttgtctattt ttactcaatt
     4141 agtcatgcaa tttaccaggc aaaaattaac agcaggaaaa cttttcaaaa gcctattcga
     4201 tttaattgtt gtaaatgcaa ttctagcgac tttaataaaa caaattgaac aatctggaag
     4261 aacaacaaaa ggagtgaagg attttttatt gacgtttatt cactttgtaa atggacacat
     4321 gtatttgttt gaagtgggta ttattctctt ttttgtcggg tatgtatcca cgaaacttat
     4381 taaacgttca attgaaaaag aaattgaaat tgacctgatt ccgacacttc ttggttatga
     4441 gtacaatgtt gattttggaa gatatgacga attgatcaat acggatatcg ttcacacgag
     4501 tatccatgaa tctactgtta agggcatcag atctatttta aaaaatggcc aggaaatgaa
     4561 agcaatagat cgaagaaaag agatctacaa tttttatgaa gtcgaagcaa gcctacgata
     4621 tggcatcgga aaattgtggt tagatggaag taatattcgt caaaacaaac catacatttt
     4681 ttggaacagt agtgatttag aacttccaat gaaagataaa taattatgga gaatcgtttg
     4741 aaaatgattc ctctatacag gattgtgctt atagttggca tggagttaaa aaccttaaag
     4801 aaggagcagt tattcaaagt gaaagaacgt cataatcagt tactatgtag taaaacgggt
     4861 acgatgaaca ttgaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg
     4921 ttgattcgaa agcaacaaga gcaagaaaaa aattaatggc tacttatagc caattgattg
     4981 atgaatggga acaattcaag gaatctaaaa ttccagaacg tctctaaaag cctttatttg
     5041 taaagagcat aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc
     5101 tattacgttt ctcaaaaata aatgtgggaa caaaatattt aaaaatagga tagtgaaagg
     5161 atagtgaagg atagtaaaag gagggtagta ggatagcaaa aaggataggc tccttttcgc
     5221 tatccttttt aaattggagg aattaatatg gaagtaagaa taagaaatgt ggatgagaaa
     5281 atcgtaagag aaattgatcg aagatgtgac gaa
//
LOCUS       DASJSZ010000131         5306 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.137, whole genome shotgun sequence.
ACCESSION   DASJSZ010000131 DASJSZ010000000
VERSION     DASJSZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.137"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..279)
                     /locus_tag="U4B03_002665"
     CDS             complement(<1..279)
                     /locus_tag="U4B03_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2069464.1"
                     /translation="MNDFTTEILKTLANKGDLNGLFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAY"
     gene            736..2247
                     /gene="gadC"
                     /locus_tag="U4B03_002666"
     CDS             736..2247
                     /gene="gadC"
                     /locus_tag="U4B03_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HEN2069465.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            2260..3660
                     /locus_tag="U4B03_002667"
     CDS             2260..3660
                     /locus_tag="U4B03_002667"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEN2069466.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3825..5260)
                     /locus_tag="U4B03_002668"
                     /pseudo
     CDS             complement(3825..5260)
                     /locus_tag="U4B03_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         1649 a          885 c         1011 g         1761 t
ORIGIN      
        1 ataagcagga acagtctgtt gcttgaactc gccattgcgg tcgcgcggaa tctggagatg
       61 aagttcccca tactcggtct tgaccgtacg gtcataggag ccgttacgag aattacccgt
      121 gttaaaacca atgcgatcgt acttttcgta atcgaggaaa gccgttaact ccgttttgag
      181 aagcgtattg acagcttttt ccaaatggac acggaataat ccattcaaat cgcctttgtt
      241 cgctagagtc tttaggattt ctgtagtaaa atcgttcata tggaagtcct cttttctgtg
      301 aatgtgttgt ggtaactcta ttctacagaa gggacttcct tttttctatt tacacaaaat
      361 attttacact ctcaacaaaa gtatcttcga ttaatacttg ctcaagttat gtgcagctac
      421 gaaaaagtag gtaccgaggt actttctttt tcgcagcttt ttttggactt atgagaagta
      481 cattcgctgg aagaatacga tataaaacga gctttttcac taaagatatt aatagagtga
      541 aagaaacaag cttttgttgt cagttttttc acttttctaa aaatgaaaat aaaaaattct
      601 ggaagtggag aagaattttg cttgctatac tttgtcttgt agaaaagagg tagacaaaat
      661 aatgcaagct caaataagcg attttccata accttggtag gaatgacaaa ttaaatagga
      721 aagggaagta ataatatgaa taaaaaaaag ttatctctat tcggtttttt tgccctgaca
      781 gcttccatgg ttttgactgt ttatgagtat ccgacctttg caacatcaca attacatttg
      841 gttttctttc ttcttttggg aggtatttta tggtttctac cagttgcttt atgtgctgca
      901 gaaatggcaa cagttgaagg atggaaagat ggtgggattt tcagttgggt tagtcaaact
      961 ttaggagaac gttttggttt tgcagctatt tttttccaat ggtttcaaat tacagtaggt
     1021 tttattacaa tgatctactt catactagga gcgctgtcgt atgtaattaa ttttccagag
     1081 cttaatacag atccatttat caagtttatt ggtgtattag tcattttctg ggtactgact
     1141 ttttcacagt tgggcgggac caatcgtacc gctaaaattg ctaaagcagg atttgtcatt
     1201 ggaattgtta ttccttcgat cattctcttt ggtttagcag cagcctactt tatcggcggt
     1261 aatccgattc aaatggctat taatgcgaaa gcttttattc ctgacttttc tcagctttca
     1321 actttagtag tatttgtatc ttttatattg gcttatatgg gcgtagaagc ttcagcatca
     1381 catattaatg agctggaaaa tccccaaaga aactacccat tggcaatgtt tttactagtg
     1441 attttggcca tcgccttaga tgcaatcggc ggtttctcag tagcagcggt tattccacaa
     1501 catgacttgt ctctcagtgc tggtgtaatc caaacatttg aaacgcttat tttacatttt
     1561 aacagtcatt tacattggtt ggtaaaagtg attgctctca tgattgcctg tggcgttatg
     1621 ggtgaagtta gttcttgggt agttggtcca tcacgaggga tgtttgcagc tgctcagcga
     1681 ggtctgttac cagttttctt ccggaaaact aataaacaag acgttccagt tccgttgatt
     1741 atgatacaag gaattatcgt tactatttgg gcagcagtct taacttttgg tggaggaggc
     1801 aacaatttat cattcttagt agcaatctcc ttaactgttg tcatttatct cgtaggatat
     1861 ttactatttt tcctaggtta ctttgtatta gtttttaaga aaaaagactt aaaacgaact
     1921 tacaatgtac caggaaaaac tgttggcaaa acgattatcg gtggaattgg ttttatttta
     1981 tcagtgttcg cattgcttat ctcttttgta ccaccagctt caattgcagc tagtgagcac
     2041 cgaacatacc aaatcatact acttattagc tttgtagtga ctgttctttt accttttatc
     2101 atttatgaag tacatgataa aagtgctcac acaactattg aggaacctac tcacgtgaaa
     2161 gctgaagacg ttaatcccgc tgtttatcca gctgctagag gagagcacca cgttacaaaa
     2221 aatgatgaac atattttaaa acattaggag gaattcatta tgttatacgg aaaagataat
     2281 caagaagaaa aaaactattt ggaaccaatt tttggctctg caagtgagga tgttgacttg
     2341 cctaaatata agttaaacaa agaatccatt gaaccacgaa ttgcttatca attagtacaa
     2401 gacgagatgt tggatgaagg aaatgcgcga ttaaacttag ctactttttg tcaaacgtat
     2461 atggaacctg aagcagtgaa attgatgacc caaacgttag aaaaaaatgc aattgataaa
     2521 tcagaatacc cacgaacaac ggaaattgaa aaccgctgtg taaatatgat tgctgattta
     2581 tggcatgctc caaataatga aaaattcatg ggaacttcaa cgatcggctc ttcagaagcc
     2641 tgcatgctgg gtggtatggc catgaaattt gcttggcgta aacgtgctga aaaattaggt
     2701 cttgatattc aagcaaaaaa acctaacctg gtgatctctt ctggttacca agtttgttgg
     2761 gaaaaattct gtgtatattg ggatgtggaa ctgagagaag tcccaatgga tgaaaaacat
     2821 atgtcaatta atctagatac tgtcatggat tatgtggatg agtacacaat tggtattgta
     2881 ggtattatgg gtattactta cactggtcgt tatgatgata tcaagggtct gaatgattta
     2941 gttgaagctc acaataaaca aactgactat aaagtataca ttcatgttga cgctgcatcg
     3001 ggtggctttt atgcaccatt tactgaacct gatctagttt gggattttca attgaaaaat
     3061 gttatctcaa ttaattcttc aggtcacaaa tatggtttgg tatatccagg tgtgggttgg
     3121 gtcttatggc gtgaccaaca atacttacca gaagaattag tatttaaagt tagttactta
     3181 ggtggagaaa tgccaactat ggctatcaac ttctctcata gtgcagcaca actaattggg
     3241 caatactaca actttgtacg ctatggcttt gatggttatc gtgatattca ccaaagaact
     3301 catgatgttg ctgtttattt agccaaagag atcgaaaaaa ctggtatttt tgaaatcatt
     3361 aatgatggat cagaattgcc agttgtgtgc tataagctga aagaagatcc caatcgcgaa
     3421 tggacactat atgatttatc tgatcgtctg ttaatgaagg gatggcaagt cccagcctac
     3481 ccattaccta aagacttgga tcaattaatt attcaacgct tagttgttcg agcagacttt
     3541 ggaatgaaca tggctggtga ttatgtacaa gatatgaacc aagcaattga agagttgaat
     3601 aaagctcata ttgtttatca taaaaaacag gatgtgaaaa cgtacggctt tactcactaa
     3661 aattcttcag agaaatataa aagaagttaa ttaactaata aaaggagttg tgattaccac
     3721 acagctcctt aaatctattt gaataaatat atgatattca tttgttacac tctcaatctt
     3781 agctgttgtt ctattcatca ttactcttat tgtaagtagt tcagtcaaat ttgcttgttc
     3841 aattgatcga taacagtatg aattgtttga aaagtaatcg gatatataat tgttttctca
     3901 ctacctaagc cttcttggta taaagaacta tcgcaaagta taatccgctg agtacaaaag
     3961 aaagtgtcgt ctactatttc tctaagttgt ttacaaatga tattactaat agaaaagtgt
     4021 tctcccaaaa tgtcttctaa ctgttgtcga tagtataaat acctaaagtc tgacggagct
     4081 actatgaaca cttcaattgg agatttccgt ttatgctcat ttaaaagact taaactaatg
     4141 gttagtttac gaatagaatt ctcattccaa cgcagaaata aattgttttg ttttcgccat
     4201 tgttcaagaa tttcatgaac ttctaagtac aaagcgcgtt gctcttcatt taacaagtaa
     4261 gtcttatcag gttgaaatat ctgaatatca gcccaaagtg agcggataaa cgggagaagt
     4321 gctttatcaa aaagtatctg attttcttca gatatattca gagataagat aagtttttga
     4381 ctgagctcaa tcaccaaagg gttatctgct acatggtttt gataaacaac gttcaagtct
     4441 ttttgtaaca attcaaagtt atcgcataga taatttctag agttgaatag aaagtagata
     4501 taaaggcatt catcttcgtc atgacataaa gagaaagaaa gttgctctaa tcctttctgt
     4561 attagctgaa acaagggagt atgtcgggca actctttttt cttcctgact aaaatatatt
     4621 gggttagtct tagatctctg caaaccgata gcgataccta ataaaataaa ttgtttttca
     4681 actgtagggt aacttcttga aaaaaatgtt tttctacata atcaatcaat tgttgaccgc
     4741 tttgaataat tgatttattt agctggatca actctgtccg atttgtctca tagattatcg
     4801 ttagtaacaa acttcgataa tccttttcag gaatttcaaa atggccatca gcatttaagt
     4861 actgcatttg tttaaaaaaa ttaatcaact catttcgtaa gttgtaaact ttgcttaacg
     4921 aaatgaaggt agtatcagct atattactcc aattgtgttc ccctgataaa taatgtccta
     4981 gtacattgaa aaagtcagag cgctgataga tgagttgagt gagtgccaac ctcgaaaacg
     5041 aagagtcgaa tgcgactgta aagctacttc tagatttctc cactgaaata atttgctgat
     5101 ggagtatgtc ggtaatgcat tccaagtcgt tagtgacagt gagtgggcaa acgttcaatt
     5161 gatctgctat ttcgcgaatc tgaatggtcc ctttttcgtg taattgctca caaatgtaaa
     5221 cttgacgaaa aatacttttc tccagatagg tttccaacat agtactcctc ctagtcattt
     5281 tatacataac tattataact ctacca
//
LOCUS       DASJSZ010000132         5134 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.138, whole genome shotgun sequence.
ACCESSION   DASJSZ010000132 DASJSZ010000000
VERSION     DASJSZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.138"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..378)
                     /locus_tag="U4B03_002669"
     CDS             complement(<1..378)
                     /locus_tag="U4B03_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HEN2069467.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYV"
     gene            complement(676..1611)
                     /gene="murB"
                     /locus_tag="U4B03_002670"
     CDS             complement(676..1611)
                     /gene="murB"
                     /locus_tag="U4B03_002670"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEN2069468.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1684..2604)
                     /locus_tag="U4B03_002671"
     CDS             complement(1684..2604)
                     /locus_tag="U4B03_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2069469.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2717..3469
                     /gene="xth"
                     /locus_tag="U4B03_002672"
     CDS             2717..3469
                     /gene="xth"
                     /locus_tag="U4B03_002672"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN2069470.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            4333..4866
                     /locus_tag="U4B03_002673"
     CDS             4333..4866
                     /locus_tag="U4B03_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEN2069471.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1511 a          993 c          881 g         1749 t
ORIGIN      
        1 tacataggct cttgatatgc ttaaaaaatg attctctgca aatttttcga tcgttacttt
       61 tgaatccacg atctccgtta acagtcgagc aatttttgat cgttgaaagt agattcttct
      121 taaatttttg atatccgtca aattcagacg ttcgacatgg atctctgctt cttctgaaat
      181 ttggatctct ggctttgttt tgaattcagt aaattcttta tttaattcta tgatataatt
      241 tcttgtttta aatttagaca aacctaatgt ttcatataac ttaggcagca ctacataatt
      301 ctttttctca aattctaaat atctagctag cagcagtttt tctctatctt cctcttctat
      361 aaggtaatcc aacttcatat cctacacctc aaaaaatgcc tcataccaat tacttgatat
      421 gcagatgaaa gttcatcttt tcacctatac tgattttatg atggattatg cttgtttttt
      481 catcagttat tctcattttt tatctttttt ttgagaatat tgcaaaaaat ggaaaaattt
      541 atgaggaaat attctatatt ttttgtttta ttgtgttaaa atgtacaaga tttgcgccat
      601 ttgcgtaaac aaccacaaaa aagagcagga cggccaccgt cctgctcttt tacttatttt
      661 aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg cttgtttttc
      721 tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga ttacttcttg
      781 gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat tgacgatgaa
      841 tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca atcctgcttg
      901 ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc cgcatgaagg
      961 gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc ttgctttgat
     1021 tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag cacgtaattc
     1081 ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag ttttcaatgt
     1141 tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc cgccatacgc
     1201 acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag caaattcaaa
     1261 tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg cacctgcttc
     1321 cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca tgatgactac
     1381 accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata ccatccaagg
     1441 tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt ttttcgggaa
     1501 tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca atggttcatc
     1561 aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca ctgaggattt
     1621 tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc ttgccagtct
     1681 tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt catgacttct
     1741 tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga tctgagaaaa
     1801 gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc agtcgccaaa
     1861 ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat cgtttacttc
     1921 ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac ttgctaaatc
     1981 catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat attccaaaaa
     2041 ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta ataaatacac
     2101 agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg tttcttttgc
     2161 cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt tcaattcttc
     2221 taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt tcttatcagc
     2281 taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt caatataaac
     2341 atggatacca ttttctagac attttttggc taattcataa tgtacttttg ttgctgcgtg
     2401 gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat atagatgttg
     2461 gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg caaaataaaa
     2521 atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg cgatattccc
     2581 tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt actttcatct
     2641 tacaccattt ttttctaaaa tgaggtacaa tagtatcgca aaggtaagga aattaattcg
     2701 aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc gtgccatcgt
     2761 aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct gtctgcagga
     2821 aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc aatattggaa
     2881 ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac cagcattgaa
     2941 tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga ttactttgga
     3001 atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg aattaaaacg
     3061 tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa atctaaaaaa
     3121 acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga ttgatttgaa
     3181 aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag cggctctttc
     3241 tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa cccaggaagg
     3301 tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg gttggcggat
     3361 cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa agatccatac
     3421 ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa gaggttgtga
     3481 aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt ccacattttg
     3541 cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg agagattgtg
     3601 aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt
     3661 tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta ttaagaggtt
     3721 gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
     3781 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag
     3841 gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     3901 tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattga
     3961 gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca aaatagttcc
     4021 tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca ccgtttagtg
     4081 gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg aaaatggttc
     4141 ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca tctaactcca
     4201 aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat aaaaaattct
     4261 atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat ttcccaagga
     4321 ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca tcagccttat
     4381 agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga tgaattctat
     4441 acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca cggaatccat
     4501 caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat ccaaccatat
     4561 tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa ggtcttagcg
     4621 ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg tacagtgaaa
     4681 agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt gtgcgaaaat
     4741 ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc atgtgcggaa
     4801 atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact tgtgactata
     4861 aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt tcactccgag
     4921 tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt gactggtgac
     4981 cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct gttctgcatc
     5041 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg ttggacttat
     5101 taccgcagat gcaagctttt ggacaccgtt tacc
//
LOCUS       DASJSZ010000133         5043 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.139, whole genome shotgun sequence.
ACCESSION   DASJSZ010000133 DASJSZ010000000
VERSION     DASJSZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.139"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..170)
                     /locus_tag="U4B03_002674"
     CDS             complement(<1..170)
                     /locus_tag="U4B03_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEN2069472.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTS"
     gene            328..591
                     /locus_tag="U4B03_002675"
     CDS             328..591
                     /locus_tag="U4B03_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069473.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            615..803
                     /locus_tag="U4B03_002676"
     CDS             615..803
                     /locus_tag="U4B03_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069474.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(886..1071)
                     /locus_tag="U4B03_002677"
     CDS             complement(886..1071)
                     /locus_tag="U4B03_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069475.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1286..1657
                     /locus_tag="U4B03_002678"
     CDS             1286..1657
                     /locus_tag="U4B03_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2069476.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1807..1879)
                     /locus_tag="U4B03_002679"
     tRNA            complement(1807..1879)
                     /locus_tag="U4B03_002679"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1844..1846),aa:Gln,seq:ctg)
     gene            complement(1925..2353)
                     /locus_tag="U4B03_002680"
     CDS             complement(1925..2353)
                     /locus_tag="U4B03_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069477.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2413..3171)
                     /locus_tag="U4B03_002681"
     CDS             complement(2413..3171)
                     /locus_tag="U4B03_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN2069478.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            3466..3663
                     /locus_tag="U4B03_002682"
     CDS             3466..3663
                     /locus_tag="U4B03_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069479.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3769..4251
                     /locus_tag="U4B03_002683"
     CDS             3769..4251
                     /locus_tag="U4B03_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069480.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(4309..4905)
                     /locus_tag="U4B03_002684"
     CDS             complement(4309..4905)
                     /locus_tag="U4B03_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2069481.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         1641 a          788 c          970 g         1644 t
ORIGIN      
        1 gacgttgttt cccaatgcct aaaagaaaaa atttgataag taaaatatac agcaggtaaa
       61 gaggccagta aaactgaaaa cttctttttt gtccatttta tgtaatagaa aagaatgctt
      121 atcataataa aaaaaggaat ataaaaaaca aattgaccga tcgcgatcat actattgcct
      181 ccgataaata aagtccatga tacaaaataa cactttgtgc gaaatggcgc aagcagtttt
      241 gtggatttat ccggtaaaat ctgtatcgat tttgaaatta gaggagaaac ttgctataat
      301 caaagtgaaa gaaggagtgg gattttcatg gatcatgtag tgttagaaat cgatttgcat
      361 ttgatcaata atattcgggt gatttactat gttgtaagta acagtgtcga acaacgtgta
      421 ctgacgaata aaataaatgg tattttagca aagaaagacg ttcaccgatt caataatggt
      481 gaaggaagtt attatagtat cccagtagaa aaaattattt atacgacagt caaagtcaga
      541 gaagatctag ataccaaaaa agcatatgaa cctattttta caacttattg attgacagca
      601 gatggaggaa aaaaatgaat ccatatgaac aacttattca agatttgatc gatggaaaaa
      661 tcgaaaagat tgatgtaaaa agagaagaat taatggcttt tcgagaagcg tggctgaagt
      721 tggagaatcg taaatatgtg gtgggaaaag cctcactgaa cggcaatgtg acttatcatt
      781 acgatccgac acgtttattt tagtatgaag tattcagaaa agaaccaaag gaggacgatg
      841 cagcagcatt gtcctccttt gattttacaa aagtgaaagt tttacttatt tgaatagatt
      901 tttaaatttg tttttgacac tgtcgatatt tacatctttt aaaagatttt gggctttctc
      961 tttataaggt cctaattgtt ctttatgttc ttcgataaat cgttttgcgc catcgaaatt
     1021 gccgttctta atcatttcct tgactttttg aaataattct tcttgattca ttttgaacac
     1081 atcctttgct atcaacatga ttttttacat ggacaaaacg agaaaaactg atttatccac
     1141 acgtcaataa tatcaaataa cgatcatttt tactaaaaaa acactcgatt ctcgcttcta
     1201 aagtaggaga aaaatctcca acttgggacg gatggaattt ctttaactga cccatatact
     1261 gaatatataa caaggaggta gaaagatgaa agtgattaaa tatttgattg gactggcggc
     1321 gattagtggt attttgctat ttggactgaa atattatact gaaggaagtt atggagaaat
     1381 ccctggtata cttgatcagc taaatccatt agttgaaaaa ggagaggtct atgtaaagac
     1441 acaaaaacca gaggaagtaa atgaatatgg tacagcaagg tatgtgcaga aagctgctga
     1501 tgccaatggg aaagaacgaa cggttgaatt taatggtctt tctgtgttaa aagaaaatca
     1561 ttatttgaaa attagcaata aaggagcaca catagaaacg tatgaggaag ttcctagaga
     1621 aaaagttccg gagaaagcat tagcaattat tgggtaaatc agtttttgta ttaagaatac
     1681 gcccgatttt attattcata atttaattat gtaaactaaa tacaagtagt aaaaataata
     1741 caataatccc cctgcaaaaa gattgttctt ttacaggggg ttcatttttt cagcaagcac
     1801 atcaaattgg gatggcaggg ttcgaaccta caattgcttg attcagagtc aagtgcctta
     1861 ccagtttggc tacatcccat ttattcaaca aaataatcat agcactttcc taaaaaaacg
     1921 caagttattt ttcttgatcg gatagttgtt gctttactcg taaaaggcgg cgtactagtt
     1981 tcttttctaa tccagcagga agggcaatca gttctgctac tgacaagacg gtgtccaata
     2041 actgatattc tttgacaggt tcattcataa tatgatagat gtttgctaaa cgttcaatat
     2101 attcaaagcg agtatactct gttcgagcaa ttagaaattt caataaatcg gtggctttct
     2161 cgtactcttg atctgcgtaa taaacaagag attgacgata caatttgatt tccagttcat
     2221 tgagggtagc aggtctgcga cgctcgttcc actcattaat agagacttta ccttgaacat
     2281 ttcccttgca gtcacggaca aaaataatcg gaatttcatt tggatccatt tttttatgtg
     2341 tgaatttttc cattatctac gctcccttca aaggtttggt atcttgagta taagggattt
     2401 ttgtttgaat tactagcttt tctccaaagt tagcatctgt ccacccattg cagtgaaaat
     2461 cgccgaaaaa tcgttgatcg gtactgctcg attttttaca ccatagggat cgttgacata
     2521 aatattttct ttgtcatatc cagtgactac acaagcatga acaagaggag tgactcttac
     2581 ttctccatta tttgtggtcc aagtcataaa atcagtttca tctgggactt caaaattgat
     2641 agtcacagaa gtccagacag gatttccttt agatagtgct tttttgatat catcgagttg
     2701 agcatcggtt tttggaaaag tgtgataatc cgtttggacg atttgctgag caagggaggc
     2761 aactggggga acatagacga ccatagccca gtctccgcca ctgatatcac caacaaatcc
     2821 ttcattagga tcgccatgag tcccatctgc attgaaaaca gggacgtaat ccaattgctc
     2881 agctaactgg tttttagttg tttcataacc ataaaaagat aaaagcatag aaagcgaagt
     2941 gacttcacag ccattttcta aagaaggaga atcaaactgg ctctctaatg gaacatctag
     3001 taaaacttct gctttctctt ttgtcttttt cgttttttgt gttgaacttt tttgtgcagt
     3061 gttcattgta tgttctggag aatggatatg taggtaataa cttgtaccgc ttaataaaag
     3121 agcagctgtg ccccctagaa acaatagttt tttgaacgtt tttttcttca tttgaaatct
     3181 cccttttcag ctgtttatct ttcctattat aaatgtattt ttattggaaa tggaagtatt
     3241 atattttaat atgtaggaaa taattagttt ttaaggaata tgttttaatt ttttgaaaaa
     3301 aattcttcta aatacaacag ctgttttaat tgtgaaaacg aaaacagtac gctataatgc
     3361 ctgtaaggtt gatttttaac tgtaaataaa aatcaatgag ttaaatctcc aaagaaatag
     3421 cagtggtaat gtggagaaaa gttccagcag tttaggagga ttttgatgac ggaagaaatg
     3481 attaatttag gagaacaata tgcatgcaaa ccaattggtt ttacaaaaac ggtcattggt
     3541 gaagttgtat caaaaatgac aaactgcgcc gtggtcaaag ttgcccaatg tgctgcagag
     3601 gaccaagaat tattagatga aaaagcaagc atggttgttg caaaatatga tacatttgaa
     3661 taaagaaaac aattggacgt gcggcgtgct gcaagtccaa tttctttgag aaggtaaatc
     3721 agtattcttt tcctattttc tggagaagaa aaagcgaggc gagttgtcat ggtaacattc
     3781 tttacggttc tattgttttt attcgttgtt gtgatgtttt tatttctttg gctggttcga
     3841 aaagaaatta tttatcggac tgtgcgaaat cgatgggtct acttggtgat acccttctta
     3901 gtgtcactag tcatttggta tacactcatc agtcatccta cagcagatga attggcaaaa
     3961 gggatcttgt cagcaatgat ttttattagt tttttgttgg acagtagagg aatcacagaa
     4021 gaaggacttg ttttgaatag ctttgacaaa aaaggtgttc ctttttctga gatcaataaa
     4081 attgtattat atcagccaaa aggttctaaa atcgtaaaaa tgaatttttt ccgaaacgga
     4141 tggcgaggac cgatgctgaa attttctgca tcattagaag aattagttcc atttttgtcc
     4201 caacgcttaa acgatgaggc agagattgat attatgattg atccagaata aaaacagagg
     4261 ttgcgacaag atgtttgtca caacctctgt tttttatttc aaataaaatt atccaaatac
     4321 gcggaagcga tcggcatcgt tcatgtattc tttttcctca gccggtgttt ctggtgaacc
     4381 gaatactaat tgcgaacgta gtttccagtt tgaagggata ttccattctt ttgcaactgc
     4441 ttcgtcaata actgggttgt aatgttgcaa gtttgcgccc aatcctttat caactaatgc
     4501 tacccaagta ttagctgttg cgattccgtt tgattgttct gaccaatctg ggaagttgtc
     4561 tgcatataat tcaaattgtt cttgtagacc ttttaccacg tcagtatctt tgaagaataa
     4621 tactgtacca taaccatttt tgaaaccagc taatttgttt tgtgtatttg ggaaagcttc
     4681 tgctggagtc aatgggcgaa gtgcttcttc agtgatttcc caaagttttt catgtgcatc
     4741 tccgaaaagg ataactgcgc gtgttgattg agcattgaaa gcagttgggc tttctttaat
     4801 tgcttctttg atcaaagttg ttaattcttc attagaaagt gaaacattac gtcctagatg
     4861 atagattgaa cggcggtttt tcaaagtgtc agtaaaagtt gtcataataa aatttctcct
     4921 ttgatttgcg ttttgcattt ttttaattga ttacgaaatg aagtatagca ccttacaaaa
     4981 agtaagtaaa cagatatgct cacaaaaaat aagcgaagtt caaaaaacaa ttttaataag
     5041 aaa
//
LOCUS       DASJSZ010000134         4889 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.140, whole genome shotgun sequence.
ACCESSION   DASJSZ010000134 DASJSZ010000000
VERSION     DASJSZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.140"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1528)
                     /locus_tag="U4B03_002685"
     CDS             complement(<1..1528)
                     /locus_tag="U4B03_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069482.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     A"
     gene            complement(1515..3383)
                     /locus_tag="U4B03_002686"
     CDS             complement(1515..3383)
                     /locus_tag="U4B03_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069483.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            3349..>4889
                     /locus_tag="U4B03_002687"
     CDS             3349..>4889
                     /locus_tag="U4B03_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069484.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIAS"
BASE COUNT         1642 a          800 c          706 g         1741 t
ORIGIN      
        1 cagcaactcc tttgactccg cttatttgta ccaatccatt agcattcacc tgctccttta
       61 caggttgatg atttatttct acattcgttc ctaaataagc atatacagga agtgtgatta
      121 cttggctctt ctcattagtt actctatata tgatagaact accagttgtt ttataaggaa
      181 ggtttaccca ttgactgcca atctttgctt cgtgttttat tatatgctcc ttattctcca
      241 atgaagcgat aggagaataa tcatataaaa aataatccat tcctttagtg gtatacgcct
      301 ctactgtttc tttgtttaca acaatatgtc ccggctgaga aattattttt tgattgaaat
      361 tttttttact agaaacagtt aaagtgatta ataacaaaac agtcataaat aaataaaata
      421 gttctcttgt cttattgtat cttctatttt tgaaaaatat catcgttaat atcagagaag
      481 agaatattat ttgcaagctt aagatccgcc aaggaaattg taacaatttg gctggcgtat
      541 tttgcagaag ataccatggc accaaactag ttgtagctag ccaagtcaaa cacgtaccat
      601 aataaatctt tttttctatg tttgttaatt ttttaaataa aatcagggga atacataaag
      661 ataacagacc aactagtccc attgagtaag tagttaagtc gttgttcata gcttcagtca
      721 gactatcgaa cacattagat gcatgagcat ataaatttgt cacgctaggt ttgttgattt
      781 tttggtagag catttgttca aacatcggat accaaaaagc actcgtcaga accaatgtca
      841 ttcctgccgc actaaataat tttttaaata tagcaataaa atctgcattc ttgaaacctt
      901 tagaaaccaa acgaataaaa ataaaaatga taattaatgc tgtactcatt aatgttgata
      961 gtacatggct gtacactagc aaactcatac tcaatgctag ctttacagac ggatactttt
     1021 cttcttttcc agagatgatt tgataagcat aaaataatac agggataaca agtgttattg
     1081 cgatagcttc ggcaattgcg gaacgatagt agatgtctac tgtccgataa gcagagaaaa
     1141 tatagagaca actaaataaa aatgccgctt ttttttgttt taaaaaccgc tcgccataat
     1201 gataagctac gagtgttgta cacaagttca ataaccaaac gtaaataata tagcttacaa
     1261 tcaaattttt tgtcatccaa tataacatta cagctggaaa aaaagtcaga aagggataaa
     1321 aataatttac accatatcca gtataattga atgtctcata attaatcgga ctggtaaaaa
     1381 tagtcgataa ccccttgatt cgagataagt gaaaagaaag atctccagtg gtataaatac
     1441 tctgtgtatt tttgaaaaag atgaaagaat atatactact aactataatt aatccactca
     1501 atattactac ttttttattt attttcattc ttcttgttta tctccttttt agtcacatat
     1561 cgagaatctt tcaacttatt aggtgcaaat cgtttaaaac ttgaatacac cactaaacaa
     1621 taagtcagaa tactgatcca taaagccatg agagcagttg gagtatattt tgttgtaatt
     1681 tctatcttat tttctccagc atttacatcc acattcacta agccatgttt tttataagag
     1741 tcatttacga ctttaccgtt tacttttacc tgtgtaccta aataagcata tactggtact
     1801 gtcacttttt gctttttgtt gcttgccact gtgtaagtaa tgttattatc tgcagcttta
     1861 tacttccctt tttcccacga ttctcctact tttacttcat gtttttctaa gtgaggcctt
     1921 tcttttatcg cttctgttgg ggcgtaatcg tataatcctc ccatatctga tgttgtataa
     1981 cgtgggagtg tttcttcatt gataacaata tgaccgtgtt ttgattggat aacttttgcg
     2041 taattttctt ttgctgctat agacgtgacg agtagaaaca ccacagtagc gccaatgatt
     2101 aaccatgaat attttttatt catagtcttt ttcataaaaa caagcgttat gatcattgaa
     2161 gaaaaaaaga tttggatacc taagatacgc caaggaaact ggagtaagct tatcggtgtt
     2221 ttttgaaaca aaaaccatgg aaataaatta gttacgcata cccatgtaga aactacgcca
     2281 acatagatga ttttttcttt tttctccatt tctttcagta tgaataaagg aaaaatcaac
     2341 gcaaccaatc ctaataaccc catagtgtat gttgtaatat cgttatttaa cgcttgaatg
     2401 atactatctg aaatagaaag tgcttctagc tgcaaatctg ttttgaaggg actgtgtatt
     2461 tcttgataat tcatctgctg caacatagga taccaaaaat agctagttaa tgcacaagtc
     2521 aaaaaaacag cctttactaa attcacgacc acaaatttta tatcgttttt tatttgcttg
     2581 cttaaaaaca aacgaactat aatcatacaa gcaatcaatg cactacacaa tagtgtagtt
     2641 aagacatgag tatacacaag tagacttata ctgacagata aagcgatgac ttttttttct
     2701 ctattgaata tcaatccata agcataatat aaaacagctg ggataatcgt aagagcgatt
     2761 gcttctgcaa ttgcggatcg ataatagata tcaactgttc gataagttgc aaaagtatat
     2821 aaacaactaa ataaaaaagc agcatctatc tttttaaaaa acttttctcc ataataaaaa
     2881 ctaatcagaa tcgtacaaat atttaataac caaatatata gaacataact taatattaag
     2941 ttatttgaaa ttccataaaa aacgactgca ggaattattg taagaaaagg ataaaaatag
     3001 ttcagtccat ctccataatt gttaaatgtt gtatagttga ttggcccttc aaaaatagaa
     3061 gataaccctt tgattcgtga aagatgaaaa gataaatcac ctgttgtata aatttcttga
     3121 gcatttctca taaaatttac tgaatataaa gtactaagta agataagaac tactaaaaga
     3181 aatgcttttt tacttatttt catagatttg ttgttctcct tttttaattc tattttcagt
     3241 atactagaat tgtctttttt taataagttt tgcgttaaat ttttcatagc actagtatga
     3301 tatactagaa aagaatcaat ttttattact tttaaatagg agaaaaaaat gaaaaaaaac
     3361 aaaaaccaaa taatagatat catttttatg ttatttctaa gttttgctta cgtcatccct
     3421 ctattcatgt ctaagggaat ctatcatagt gtcaatcagg atacttactt tcatttatct
     3481 cgaatcatag gactagataa tgtatggagt tctccagtaa acttcaataa cttcgatcat
     3541 catggaacaa tgatgaatat tttttatcca tggcttactc tttatccagc ttttttattt
     3601 tataaaatga tgggaaatct tgttttaagt tataatatct actatttttt tatcactttt
     3661 ttaacaatgg tcgtttctta tttttctatg aaacaaataa aaaacaatcg ttatatttcg
     3721 ctgttatttt cgattattta tacattttcc gcctatcgtg ccattgacat attccatcgt
     3781 gcttcattgg gagaagctgt cgctttaaca tttttgcctt tgatactaat ggggtgttac
     3841 gaaatttata ttagagatta ccaaaaatgg tactggttgt ctattggcat gacgctagta
     3901 gtgtatactc atttactttc tgtcgctatg gtttctgtat ttattggagg aacgttgttt
     3961 ctttcttttt atttttggga ccaaaaaatc gctagactct taagtctttt aaaagctacc
     4021 gtcctcacct tttttttaag tgcgggattc ttaattcctt ttattcaaca atctagagca
     4081 caagaattga aagtcccact gggaaaagaa ttaagtggta tggctcctag tgatatgctg
     4141 acacatattt taaataataa ctataacaac tatactattg gattattctt atttctcgga
     4201 ttgattggtg cttttatttt tataaaaaaa ttaactgctg atgatttatt tattttcttt
     4261 cttggtgtat ttgttctttt ttgttcaacc aatttatttc cttggcaact ttttaaccat
     4321 acaccagtta agagtctaca atttgtctgg cggttaaatg gtttttcttc tttatttata
     4381 gcctatacga tgagtatagt gatctattat attttttcta ctaaaaataa ttcatggaaa
     4441 ataatggtct ttacgtttct tgcactaata ttgcatctct caggagtaag caattctatc
     4501 catgctaaca aagattcatt aacacttata gcacctgaag acgctgttgc tatagctgaa
     4561 aattacaatc atactgatta tgcaaacaaa gagtcaatct atcatcctga tatgattaat
     4621 aatgatcaat atttactagg taaccaggaa ataaatccaa caactcattt tatgagtaac
     4681 aaacttacaa ttgaattaga taattcgaat aataaaaata ctgtcttgac gacacctatt
     4741 tatcgttata aagggcaagt ggcttctatt aatggcaaac tagtacaaac aaaattatct
     4801 aagtttggca caactgaatt aactattcct ccaggtataa ataaagtcgt tattacttat
     4861 cagtacacaa aactagcaat tgcttcaag
//
LOCUS       DASJSZ010000135         4790 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.141, whole genome shotgun sequence.
ACCESSION   DASJSZ010000135 DASJSZ010000000
VERSION     DASJSZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.141"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(220..450)
                     /locus_tag="U4B03_002688"
     CDS             complement(220..450)
                     /locus_tag="U4B03_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069485.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(544..882)
                     /locus_tag="U4B03_002689"
     CDS             complement(544..882)
                     /locus_tag="U4B03_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069486.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1810..1926)
                     /locus_tag="U4B03_002690"
     CDS             complement(1810..1926)
                     /locus_tag="U4B03_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN2069487.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(2402..3280)
                     /locus_tag="U4B03_002691"
                     /pseudo
     CDS             complement(2402..3280)
                     /locus_tag="U4B03_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
     gene            complement(3603..4628)
                     /locus_tag="U4B03_002692"
     CDS             complement(3603..4628)
                     /locus_tag="U4B03_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2069488.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT         1624 a          885 c          719 g         1562 t
ORIGIN      
        1 tatagaatca ataagctaat taagaaacaa actttagaaa ctctagaaga acttctgaat
       61 ttatatagaa gtgtgtagct acttactaaa tatacaaaag ttaaaataaa atactctgga
      121 tactctttat actgcattaa agttagtaat attaacagca aagatatcga aaaaacaata
      181 acataatatg tgtcaaaatt tttcataata agaactccct tactgttgca tactaacata
      241 atccaaaaac tcttctactt cagctaaaga cattccactt tctttagtcc aaaaaacagt
      301 tttttctatc gatccttctc tgtaaactgt aaatgtcgga gtcgagttaa ttgagtagcg
      361 actcttgaat tctttccact cattttcatc ttctctaact tttgctacat tcacgtaata
      421 aatattttct tttccctttt ctaccagcat atcgaatagc ttcctgcttt tgatgatcga
      481 tttcttgtgc tacatctagt aaattttcat caatttctat atcataaagt atttcactag
      541 tgtctacatc cacaatatat actttttatc agtagcaatt tttgagtaat ccgttaaaat
      601 gatctcagtc tcgtcacttt catcagtata aatatacgtt aacaatgaac tcaccttttc
      661 aatcggtgtt agctctaagc ctactgtatt ttttactatt gagtcattat ttaccaccat
      721 aacttcgtac tgtatatacc atcctatttg gtttccatca atatcagtaa ttggctcact
      781 agctaatgga gtctccggaa caatattttc ctgatatttc ttaaactctt ctgtagcacg
      841 aaaaaggtta tcatgttcct cacttgaaac tggttgagtc atagaaaatg ttacttggct
      901 aaacagaggg aaaataaaca ctacaatcaa caaagtataa aaaaaagccc ttctttttca
      961 ttttttatct tccaatcaca aattgaaaaa cttattcctg caactttgag tctagtatca
     1021 caatcgactg actacaacta ttcatttaca ttctacttta agttcctcct taaccattaa
     1081 ttttattaaa gatacttgta atagtgattc tcacaaaatt tcatgaaaaa tactttatta
     1141 ctgtatctaa aattccaata cacaacttat cgactattat ctgaaaacca aaacttatta
     1201 ttccttgatg ttattctagc aatagtaact gcttttttat ttttttgcat attttttcgg
     1261 cgttactttt taaagttaac tttttcgaca aatagaaaga gatacgaatc actgattttt
     1321 ttgaagaaaa gaaatggagt gagctgaacg ttagagatat tgggagcttt cgtaactgct
     1381 tattaaacga taatggttat ttgcaaccgt attgctcgct tctacatgaa atatttgatg
     1441 aaatatttcg caaaaccttt gattatgtag ttacgtttcc attcttgact aaaaatgttt
     1501 caaaaacgaa caaaggtgtt aaaaggtaaa gtgatcaata ctactggtca aaataagagg
     1561 ttgaaaggga attgaaaaat aactacagat aatactttta taaacgtacg tgctttgtgg
     1621 ttctgtagtt aactatataa ctggaattag ggaatggtat caatttgaaa agaaagattc
     1681 taccaattta tcatatgtta aaaatggaaa atcaacagca gttctttcgg aaaccttaca
     1741 caaaaaccga ttattgaaaa tagccgaaaa acgtttttcg gctatttttt taattaagtt
     1801 tccttcttct taccttttcc cactattccg tttcacatct ttgatgatct tcagcgcacg
     1861 ttttcgtgtt ttgatatttg ggcttttcaa gcggcggtat gcacttgaga tatctgtttt
     1921 ttccatatgg acgacccact ttcttctttg attgtagtca tgctaaaaaa cacaaatcga
     1981 aacaattacg atttgtgttt taaatggtat caaaagttaa aatgaaagtc aacttgttaa
     2041 aacagtcgat cagactagct gattgactcc tatctctgga aataaatttt cattattacg
     2101 ttttagctac ctaaaaaagg ccctcttgtc cgttaacttt aatatctatt gttatagcta
     2161 gtttacctat tgaacgtact gtgtgttcga tagaacacta aaaaagaggc tgggacagaa
     2221 gcgtttaact ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa
     2281 tttcagctta tttccgaagg agttgcttct gcttccgccg tttatacgtg tttagagcgt
     2341 gagacaaaag tgttctttac ttttgtctca cgctctttta aactatttcg aagtactaaa
     2401 cttatgaagt aagaatacct aagccagagc caatgtgctc gcctgcaagc aattgctctt
     2461 ttgcttgtcg ctgtaaactc gtactttctg gataatcatg tatttgggca tctactcgtc
     2521 caaagatcca agggactcca ctatcgtcct gttcatcaac tgataataat cgataatcgg
     2581 ctgcaaaaag acagattttt gactcttttt tcaactattt agtaagattg ggtgatagta
     2641 accaagtatc tgtgtacatc acttggtaat ggtaatcagg aaaataaagt tcaaaaaaac
     2701 gaatggcact ttcgtaacta tctcttatct tattctgaga taaatctgca tcggaaggaa
     2761 tatgaagaga aaggcactgc tttccttcta atcttagatc cgtgtaccct ttcggaaaat
     2821 atgtcatttc gtactccaat tcaccaatcc gaaagatcaa gccactagtg taatgccaaa
     2881 tccaaaaacc acggtcaaag cgatagcttc ctgttacttg gaaatgttca tgaataaatc
     2941 tagtaaaagc attcattgtt gccaaataga tagttcttgg aatcttcaat ggttgataaa
     3001 tttttttcca actttgagaa gctgcatata gataaatagc taatgctgca aaaggttcct
     3061 cttcatagac tttgtctatt ttctttgcag tcgcctttgc tttattagga tccattagtt
     3121 catgactata ttgctcaaac acctcatcaa ggatgttttt tgcttgtaca atcatttgct
     3181 ctataacgac tttgggaaga gttaaagttt ctgccagaat ttggatttca ttgcttattt
     3241 tcttcatcaa atttcccctt ttcatatctt tgaacctcat tgtttaaatg actgattatc
     3301 cggacaccat atggtataaa catactttta ttgtacacta actataaaac ttaaactttc
     3361 tatgaagaaa gaatatctgt aagactttca aggaaagcat tgaccatctc aggaaagttt
     3421 attcagttac catcataaac aaaatttttt gatagaataa atataaaatt tttttgaaag
     3481 ttcctttcca catttttgtg tgaatttgac ttaatgctga gaatctggct tttgaacacc
     3541 ttgaattaga ggttgtgaca aaaatcttgt cataacctct tttggcacat atatttgtcc
     3601 ttctattcta atttttgacg aacaaccgtt aaataatgtt gcaaaccaac cgtcattacc
     3661 cacgcaacac ctaaaccgat gaaacttcct atgacgatcc ctaaaaaacg atattcgatc
     3721 aaagaaatca agtatgctcc acgaataagt gtagatagca tcaacgccat cggattaaga
     3781 aaaatattag caactgcata atttctagca actagaaatt gtgcgattcc atatagaaaa
     3841 gtgatcagaa aaatggtctg caactgcgta aacggcacaa atgacaagaa tgccgacaca
     3901 cataacccac ccatcgatcc aatcagatat tggacttgtc gatgcttcat cgcatccaag
     3961 ttttctgcta gcaaaatcga tgcacatgac aaggtcaacc aataaggatt atgcagattc
     4021 aagccgtgac tgacataaac ggaaagaaat aaggcagcgc tgtagaaaac agaatcaatg
     4081 atcactaaag gctcttcgtg aaatctttct tttaaagttg ctttttcaat tgtctgctca
     4141 ggccgtgaat ccagtaattt tgtaatcact gccgcaatca gggcaaaaac gacacccata
     4201 aaaaaataaa gtgagacgat cggtaattga gcgattggca gctgcatgct cgttcccatg
     4261 gcagaaagca tcgcaaaaaa caagccacct ggtttagaaa tatggaataa tcgcaacacg
     4321 aatctgctgg aaaaagcaac caaagctacg gcaaatggtt caagccataa aacataagtc
     4381 gataagatcc ccaaaacgaa agcaaccaac agtccgcaac cgatcagaaa catacgtttc
     4441 agcaaatatt tcatcggtat gttttgataa tagaaaaaag taaagatccc taatgatgaa
     4501 aaacttgaaa tcaaaaaatc atgatttatg taacctaacc ataatacaat aaagataagt
     4561 agcatcagat tcatgatacg aaaaggatta tcttgtattt tattgaaacg aaaataagtc
     4621 ttgaacaatc tatccctcct tcctagtaat tggaaaaaaa gctgaaccaa gatgaaatgc
     4681 caatcccgtt tgtcagcatt acctagtata acaactattt cttgattttt cataaactta
     4741 gtatcaaaaa gtaaacaact tggagaaatt agaacaaaaa acgaacattc
//
LOCUS       DASJSZ010000136         4727 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.142, whole genome shotgun sequence.
ACCESSION   DASJSZ010000136 DASJSZ010000000
VERSION     DASJSZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.142"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U4B03_002693"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U4B03_002693"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEN2069489.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="U4B03_002694"
     CDS             complement(1297..2133)
                     /locus_tag="U4B03_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2069490.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="U4B03_002695"
     CDS             complement(2317..3534)
                     /locus_tag="U4B03_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2069491.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="U4B03_002696"
     CDS             complement(3683..3862)
                     /locus_tag="U4B03_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069492.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="U4B03_002697"
     tRNA            3917..4000
                     /locus_tag="U4B03_002697"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..>4727)
                     /locus_tag="U4B03_002698"
     CDS             complement(4240..>4727)
                     /locus_tag="U4B03_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069493.1"
                     /translation="PFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGT
                     KLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSP
                     TVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVIN
                     R"
BASE COUNT         1425 a          993 c          763 g         1546 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggta
//
LOCUS       DASJSZ010000137         4685 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.143, whole genome shotgun sequence.
ACCESSION   DASJSZ010000137 DASJSZ010000000
VERSION     DASJSZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.143"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            211..1149
                     /locus_tag="U4B03_002699"
     CDS             211..1149
                     /locus_tag="U4B03_002699"
                     /inference="COORDINATES: protein motif:HMM:NF016640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF616 domain-containing protein"
                     /protein_id="HEN2069494.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1395..2411
                     /locus_tag="U4B03_002700"
     CDS             1395..2411
                     /locus_tag="U4B03_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEN2069495.1"
                     /translation="MKMKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQL
                     KEDRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAY
                     IDSNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKK
                     SNELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKME
                     RDFTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFV
                     ETIEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTV
                     YYLS"
     gene            2779..4203
                     /locus_tag="U4B03_002701"
     CDS             2779..4203
                     /locus_tag="U4B03_002701"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014051.1,HMM:NF026018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEN2069496.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            4433..>4685
                     /locus_tag="U4B03_002702"
     CDS             4433..>4685
                     /locus_tag="U4B03_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069497.1"
                     /translation="MSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANT
                     LVNKDIPNNSVVAGSSCKVISTIEEHYEKNKNEFIKYRTS"
BASE COUNT         1618 a          596 c          850 g         1621 t
ORIGIN      
        1 agtaattata agagaacaaa agtaaagaat tatatctact tgcttgcgta tttctttttt
       61 atcattgtgt gtttgatatt catcaatata agaggaactg cttttgtaaa tggaatccta
      121 ccattattta gagaaaatat tatttttaag gctgtttact atttttttga gtaagaataa
      181 atagcactta aaaatagaga tgagggattt atgggaagac aagaattata tagtttagct
      241 atcaatgaat tggaaaaaag aaatcttgaa atacatgaat taaatcaaaa aaataattct
      301 attttaggca tgaaaatacg aaataaaatt aatcaactta accaaatgaa aaaacgagat
      361 aatctgttgc cagcaaaata tacccttaaa tcgatgaccg gtaaggggcc tattaaagac
      421 tattcaaaca gagagaatcg cagtgatgaa ctaagaaaaa aagagttaac taatcaagga
      481 acacctaaaa ggattgctgt gtatagttgt atcactggta attatgattc tattaaagaa
      541 ccggattttt ctcatccatt ggtagattac tatttgtata cagataatgt gattacaagt
      601 gagcattgga atattagaga gatacctaaa tatatatcgg aattaaaaaa taatattttg
      661 atcaatagat atattaaatt tcatcctaat gagctattta ctgactacga ttttactttg
      721 tatgttgatg gtaatgtaac aataacttct gatattaccg gattactgaa cagttcttcc
      781 aaacttgggt taagtatgca taaacatagt gaaagaaaat ctttatattc tgaggcagat
      841 gcattaattg cagttggcaa aggtaataag gaaagtatta tagagcaaat tagtaagtac
      901 agagaagaag gatttcctga taattttgga ttggctgaag ccacggttat tttagttgat
      961 ttaaataatt caatcgctga gaatcttcta gatttgtggt ggagagagta tttgaactca
     1021 gaaagtgggc gagatcagct cgcatttcct tacgtgttgt ggaagtccgg acatgcaatg
     1081 aaggaagtga ctaccttagg caataatgta tataaaagcg ctaaattaag aatcataagt
     1141 cattattagc cctaaatgat ttctaaacta ttctttcaaa tcctagtaaa tatttggaga
     1201 tagaagattt cctttttaaa gtcataaaaa attaagttat attttttgag tgaatggaga
     1261 agacgcatag tatcataaac ttcgttatat cttaaattat ggggtatgcc taaataataa
     1321 atgtaatacg agtataaaga agaaagcaat tttgggttta accctattgg ttcctttcag
     1381 aaaaaggtgg atgaataaaa atgaaaatat taataacagg tggagcagga tttattggtt
     1441 ttcatttgtc tagaaaatta ctcaaggaaa ctgaaaatca aattgtagcg attgataatc
     1501 tcaatgatta ttatgatgtc caattaaaag aagatcgatt aacgatactg tacgattata
     1561 ataattttaa atttatcaga atggatctat tagagaaaga aaaattagat tctctatttg
     1621 aaagtgaagg ttttgacata gtaattcatt tagctgctca agcaggtgtt agatattcta
     1681 ttgaaaaccc ttatgcttat attgattcta atattatcgg ttttcttaat attcttgaag
     1741 cctgccgaaa cttccctgta aagcatctat tatatgcatc atctagttca gtatatggtg
     1801 ggaacacgaa agtaccgttt tcaacagaag attcggtgga tcatccagtt agcctttatg
     1861 cggctaccaa aaaatcgaat gaattaatgg ctcatactta tagccacctt tataaaatac
     1921 caacaactgg gctgagattt ttcactgtct atggtccatt tgggcgacca gatatggcct
     1981 atttctcttt taccaagaat attttatctg gaaaagaaat taagattttt aaccatggaa
     2041 agatggaaag agatttcact tacatcgatg atatcgtaga gggaatcact agattaatca
     2101 ataaagtacc tgaacccaac tccgactgga cagacatcga tgatttgagt agtgaaagtt
     2161 gggcaccata taagatatac aatattggaa ataataagcc ggttgcattg ttggagtttg
     2221 ttgaaacaat tgaaaagtat ttaggtgtta aagcgaacaa gatttttttg gacatgcagc
     2281 cgggtgatgt cgttagaact tatgccgata ttgaagattt agaggaaaaa atcaacttta
     2341 aaccgaatat aaccttggat gagggcatta aaaaattcgt tgaatggtat acagtgtatt
     2401 atttgagtta attttgaaag gaatattgaa gatgggtaat tcatacgatt tttttagctt
     2461 tgttaggtat tttaggaaat tctactttca aacgtgacga actttctcgc cgaatagata
     2521 ttttctataa ctaaaagaat ttctataaaa caaaattgtg tatcaagatg tgtgaactgg
     2581 attctgactg ccaatattga ttgatgagat tgaagaaata tatagttgat catggatatc
     2641 tttatttgaa aagatcttag ctattcacat attcaaaaaa atttttacta cgaggactca
     2701 cacgatacag aggaatttag gtagttctaa ttaaaccaga aagtcatcta tagatgtgag
     2761 ttgagggagg tcatacggtt ggatagtagc agtaataaat cacattcaat agctaaaaaa
     2821 gcttttattt acgcaatagg aacattatca tcaaaaatga tatctgtcat tataatccct
     2881 atctatgcag tatatatttc tgttgccgat ttaggaagct ttgattatca aaatacaatt
     2941 gctcaatttc tttctccagt tatttttgga gcaatatggg aagcaatttt acgcatggga
     3001 ttaggcaaag aaaaaaatat tcagaaaaaa gtagtgacga acagtatttg ttttgttata
     3061 gcaaatacag tgatattagc cattttttta ccaataatat atagaatgat tttcccgaat
     3121 ttagagtatt atcaattgtt tgtttttcaa ttaattttca ctcctatcct aagtgttttt
     3181 caatatattg taagagcact aggtgaatca aagatttttg tgatgagtgg tataatcgct
     3241 gcgggtatca acctgttact ccttactatt tttgttgtta caatgggtat ggggatactt
     3301 ggtctcttac tctctatcat aatttctaat gttataaatt tgattttttt gattttcagg
     3361 gggcagatct ttcaatattt cgcaacgagt tcttttgatt ttttattttt aaagtccatg
     3421 ctgaaatact ctatcccact cactttgaat ctcacttttg gatggctaat agttggtttt
     3481 tcaagatttt atattggaac gtacatcgga gtaacagaaa atggtatttt ttcttttgcg
     3541 atgaaatttt catctataat tttaactctt acaagtattg ttagtatgtc tatgatagag
     3601 gatgcggtta tcacgtctga gaatgatgat ttcatcgaaa gattcgttga gaaaaatacc
     3661 tatattttta aaatgtacat taggttagcg actctttcta ttcctatcat ttgctgtgca
     3721 tactctttta taaccaaccc tgattataaa aaatcattag ttctagttcc aatattagtt
     3781 attatggcat gtttcagtgg gatgacaaca aatatcggaa atatatttgc agtatacaat
     3841 aaaacaaatc agttattaat gatttcagta gtgtcaggtc tattgagtgt tgttggcacc
     3901 tatttattag gctattcttt tggagtaatt ggtgtctcat ttgctacttt gctcggtact
     3961 ttagtattat ttggacttag atattatttt ggaaggaaaa ttgcttatta tgcatttgat
     4021 tggaagcgaa tatcgctact tttagttctc tattccgcaa tcgcttttgt atgcattcat
     4081 agaggcttct attttaatct tttcttaacg attattttta gtgctatttt cgtttttacg
     4141 tataaacaat taattataac gtatctaaaa aaatttttca gaataaaaat ggctaaaagg
     4201 tgagggtata cgaagtaaga atagaatctt tcgaagaata aagagaaaac tatttggaaa
     4261 aaatctgcga gaatgtatta atgaaggttt gatattcgga aaaaaacata gtttagtaga
     4321 ggttcctgaa tttgggagcg agccatattt aattgaaata ggggataatt tcagatgttc
     4381 atttggtgtt aaatttataa cacacgatgg tggagtctgg gtcttaagga agatgagttc
     4441 tgtgagcgag tcaacttcaa aaataggtag gatcaaattg ggaaataata tttttattgg
     4501 tatttactct attgttttcc caggagtttc aattggcgat aatgtaatta ttagagcgaa
     4561 cactttagta aataaagata tcccaaataa tagtgttgta gcgggtagtt catgtaaagt
     4621 gatttcaact atagaggagc attatgagaa aaataaaaat gaatttatta aatatcgaac
     4681 ctcga
//
LOCUS       DASJSZ010000138         4587 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.144, whole genome shotgun sequence.
ACCESSION   DASJSZ010000138 DASJSZ010000000
VERSION     DASJSZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.144"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            186..2855
                     /locus_tag="U4B03_002703"
     CDS             186..2855
                     /locus_tag="U4B03_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEN2069498.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3566)
                     /locus_tag="U4B03_002704"
     CDS             complement(2946..3566)
                     /locus_tag="U4B03_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2069499.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="U4B03_002705"
     CDS             3680..4219
                     /locus_tag="U4B03_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069500.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="U4B03_002706"
     CDS             4216..4554
                     /locus_tag="U4B03_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HEN2069501.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1420 a          790 c          859 g         1518 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgac
//
LOCUS       DASJSZ010000139         4489 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.145, whole genome shotgun sequence.
ACCESSION   DASJSZ010000139 DASJSZ010000000
VERSION     DASJSZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.145"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            293..994
                     /locus_tag="U4B03_002707"
     CDS             293..994
                     /locus_tag="U4B03_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2069502.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1566..2582)
                     /gene="tsaD"
                     /locus_tag="U4B03_002708"
     CDS             complement(1566..2582)
                     /gene="tsaD"
                     /locus_tag="U4B03_002708"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEN2069503.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2579..3043)
                     /gene="rimI"
                     /locus_tag="U4B03_002709"
     CDS             complement(2579..3043)
                     /gene="rimI"
                     /locus_tag="U4B03_002709"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN2069504.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3050..3592)
                     /gene="rimI"
                     /locus_tag="U4B03_002710"
     CDS             complement(3050..3592)
                     /gene="rimI"
                     /locus_tag="U4B03_002710"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN2069505.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3576..4298)
                     /gene="tsaB"
                     /locus_tag="U4B03_002711"
     CDS             complement(3576..4298)
                     /gene="tsaB"
                     /locus_tag="U4B03_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEN2069506.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1348 a          935 c          755 g         1451 t
ORIGIN      
        1 gtatacactt tcaaaaaaat atttattcat tgaaaaaaat tttttgaata aattaaaaaa
       61 ataaattata ttagcgttgt tagcgtttac tatatgtatt attgttgttt tttatacaca
      121 aatcattcgc ttcaaaacaa attattcaat accattgtat attttttaca taataacaaa
      181 ttatatttgt aaagtattaa aacttgtttc taatgttggt atgttaaaac tttacaaatt
      241 ctttaaaaaa agtgacaata aatatagtga acaataagga gaggactttt taatgcgtgt
      301 taataaaatc gattttcact tttcaaaact cagatatcaa cctgattttg aacatttctc
      361 ggatgaagag ttccaacaga tcaaggaaca tactgttctt cgttcttata aaaaaggaca
      421 gatccttttc gacgatggtg atcccagaca acggttgtat atcttgacca aaggactcgt
      481 tcgtttagaa cgttatgatg aaagtgcaac ttattactac tatgactatg tgaatacaaa
      541 taaccttttt cctatgtcag gaatgtttgg agaagagacc tacacttata cagctcaagc
      601 gatgactgat attgaaacat tctaccttcc tgtttcctac tatgaaaaat tagcacgaaa
      661 aaatcccgaa cagttgatct atatgataca aaaaatttct aaagtagttg aaatacatga
      721 attgcgtatc caaatcggat tgacctctag cgcctttgac cgggtaaaac aaaatttatt
      781 cattttgaaa gaagaattgg gcgtaccaac aaaagatggt catatcacca ttccttatcc
      841 gatcacctta aaagaattag cagtgaatag cggcacgaca cgtgaaactg ctggacaagt
      901 aatcaaacaa ctacgtgatt ccggactgtt agattatgac aaaaagcgtt ttactttctg
      961 taagacagct actactccag ctatgagaga ataacgcgaa caaaaataag cagtgaccct
     1021 gatatttatc agggtcactg cttattttcc tccctaaaac ttctgaaaga tccggcagtt
     1081 ttagatcact gggcaagact caaagatttt ctatgtgtgt ttcagttatg taatattcac
     1141 tgtttgcaaa aaatactgat agattaacta gaggaagcgt cttgtcctca tccgacctag
     1201 attattcgat attcctcgac aataaacgat taatcagtga gggcaatcag aggaacggat
     1261 aatgatgaat aagctgtaaa aaatagggct ttgacaaaag cgtgtcacag cccttatttc
     1321 gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt cataatgttc ataaacatga
     1381 ggagctgttt tttgaaattg ttccttgtga atcagagtta aacgacaggc ttacgaactc
     1441 ttgattaaac ggcgttcaaa agcttgcata cgcggtaata agaacagcca ggtcaaaaac
     1501 atgagaagcc gtttttcctg ggctgttctt attacttgat gcagggcaga tttttcacaa
     1561 cctctttatt cagataaaac caagcttgga tcagcattca actgatagct agcaaaattt
     1621 cttttttgca tttccacatg tgctgctgct ccaatcattg cagcattatc tccacaaagt
     1681 cgaagagggg gaatcactag ttctacctca gggagtttag ttgccaaagc atcctgcaat
     1741 ccttcacgca acccttgatt ggccgctaca cctccagcta caatcagttg cttgactgga
     1801 taattttgac aagctcgaag tgttttagta atcaatacat cgatcacact tgcttgaaag
     1861 ctagctgcta aatcattttt gtctagatct tctccacgct gttgtgcatt atgcaccaaa
     1921 ttgataaatg cactttttaa tccgctaaaa ctgaaatcat aattatcttc atggatcatc
     1981 gctcgtggaa aatggtaatt atcttttccc tgatgagcta gctgatcgat ttcttttccg
     2041 cttgggtaag aaagacctaa tactcttcca actttgtcat aagcttctcc tgctgcgtca
     2101 tctcttgtct ccccaatgat ttcatatgat ccatcttctt gcatatagac taattctgta
     2161 tgtcctccgc taacaagaag tgccatcaat ggaaactgga atggtttaac cagacgggct
     2221 gcatagatat gccctgccat atggtttaca ggaataagcg gcaattggtg tgcccaagca
     2281 aaagccttag ctgcagaaat accaatcagc aacgagccga caagtcctgg tccataagtg
     2341 acagcgactg ccgataaatc ttctgccgac acaccagctt ctaccagtgc atcttccaag
     2401 cataaggtga tttgttctac gtgatggcgg ctagctactt caggaactac gccaccaaaa
     2461 cgtttatgac tgtttatttg agaagcaacg atgttagata agatctctgt tccatttcta
     2521 acgaccgcta cactcgtttc atcacaactt gattcaatcg ccagaatcaa ttcttcattc
     2581 atttcatcgt ctccgttttt aactttctgc tcataatgat cgcatcttcc gtcggattgc
     2641 gataatatga tttccgttta cctaatatac gaaacttttc tgattcataa agttttcttg
     2701 ctgcttcatt agatagacgt acttctaaaa aaacatgcag cgaatctttt atttgttccg
     2761 taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa actgcaacag
     2821 caatatttgt gatctcgact tcatctatta ctttcgagta tccaataaag ccacagatct
     2881 ggttatcttc aactaaaagc agataatcag agtgaggctg ttggatgtct gccaaaaact
     2941 gcattttcgt ccaaggtgaa ccatgtggat aagcattttc gctaatcatc catacgcatt
     3001 ctgccaagta cgcttcatca aaatcttttt ttctgcaaat caactaatct cactcatttt
     3061 ctaaaagttt tatcatatcc aacgcatctt cttttgtttc agtgtaatat ctttttttga
     3121 tcgttgttgc ttgaaaacct aacttccgat aaagtctttg tgcatcttga ttactcatac
     3181 gcacttctaa tgacatcgtc tcacagcgat tcattatagc aaagttctcg atttcgtcaa
     3241 taagcagcga accgattcgt ttacgctgat atgccggtcg aacggcaata ttcgtgatat
     3301 ggcaatctaa accgaacgct cttgaaccga taaaaccaat cattcttcca ttatcaaaga
     3361 tacctagata tagatgagga agcggtgact tcaattctga tataaaggca ctttttgtcc
     3421 atggcaattc gccattatag acttctcgtt cgacatccag cagctctttg atatcttctg
     3481 tagtcaattc tcttagtacg taacgacgat cgatttgttc gattgttttt tctgtataaa
     3541 tcattttatc ttgaaaaaag cttctggcat acgctctaaa ttttttcaac ataactttcg
     3601 tctccaggtg tgtgcgtctc caaccatttt tcttctgctt ctacccgttt taaatagcga
     3661 ggaagaaaag cttgtttctc tgcaactggt tcagctaaag cacctaattg agccacagtt
     3721 acaccacttg gaatatccca tgctggaact gtattaatca tcgcactagg caatatttcc
     3781 tttatttcat agaagaattt gtgacaatca ctcccgacga aataaatagc ttcattcagc
     3841 ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta catcttcaag tacaatttct
     3901 aatctatttt ctttccagcg gtatgcccca gcgtatacat tttttcttcg agcatcaaac
     3961 atgggcacaa taattttatc cacaccgatg caattagccg cgatggtttt cagactagat
     4021 actcctacta gctctttatc taatgtataa gcaatcgttt ttgctgtcgt tacacctata
     4081 cgaagccctg tataagaacc gggaccatca gaaaccgcga ttcggtcaat gtcttttgga
     4141 cttatttgaa gatctgcaaa caattggtcg attgcaggca tcagtgttac actgtggttg
     4201 cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt cttctaccac accgatcgcc
     4261 aaagtttggt ttgcagtatc tattcctaat gtaatcatat atttgatcct ttctgctatg
     4321 acattctcgc cttccattgt agcatacttt tttattctga ggatgaaaat accaaaggca
     4381 aaactagtac aatgatacat gattatctgc tggcgggcaa attttgcccc ttcctgaaac
     4441 acaaaaaagc tgcggtatca cttcatttcc ccacagcttt tccatcttt
//
LOCUS       DASJSZ010000140         4423 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.146, whole genome shotgun sequence.
ACCESSION   DASJSZ010000140 DASJSZ010000000
VERSION     DASJSZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.146"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..717)
                     /locus_tag="U4B03_002712"
     CDS             complement(<1..717)
                     /locus_tag="U4B03_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2069507.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDL"
     gene            complement(719..2158)
                     /locus_tag="U4B03_002713"
     CDS             complement(719..2158)
                     /locus_tag="U4B03_002713"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEN2069508.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2343..4289
                     /locus_tag="U4B03_002714"
     CDS             2343..4289
                     /locus_tag="U4B03_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEN2069509.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1408 a          761 c          728 g         1526 t
ORIGIN      
        1 taaatcatca gaaacaaaaa taccatctgg tttgtgattt ataatagttg actttatttc
       61 catttcacga cggatagtag acagattatt aggaattttg aaaatttttc catttggatt
      121 ttgaaaagaa aaacctagtg cacgtaattc tgtgggagaa tccgtgttat catttcctgt
      181 aatcataatg gtattttgac aaccattttt ttgtaaagtc ttagctgcaa gttttccccc
      241 ttcaaagtta tctgaagaaa caatggaaac attaggagct aaatttctat caaaagcaac
      301 aataggtgct ctcacacgct cataatcagc aattccaaga ttatggcttg atgagattat
      361 gccatcaact tggttggctt caagcatttc aatgtattct ctctctttag caggatcatt
      421 ttgactattg caaataatcg ttttatatcc atgtttaaat aactctattt cgagatactc
      481 aatgagttca gagtaaaaga tattactaat attaggaaaa ataagaccaa taagctgagg
      541 tgactttcct tgaagtccac gtgctaaatt atttggttta taaccaagag ttttcatagc
      601 ttcatgaact ttttgttttg ttttttctga taaatatccc ttattattaa tgacgcgtga
      661 aacggttgtt gggctaactc cagctaattc agcgacatca gttaattttg ctaccatttt
      721 aatattttaa ttcaaagtat tttccagtag gttcgcctga tttgatttga ataccatttt
      781 ggtgattttc agggaataca cgactagtaa acactttttc tcctttatta ataaaaatct
      841 caatgattga gttatctaca aaaatgttag cagttgtttc tttagtatca attgagcatt
      901 cacgaatcgt accaaagtca gtggcatatt gaatgcctgc tttgctgcga tctaaaataa
      961 tttttccttc tttggtatct acaatcaagc taagtccatt tcctttacta tcagagaaaa
     1021 gaagtaactc cgatttctga tttggaggaa aagttagttc aagctcataa gtattatttg
     1081 tttctgcttt tgctgtaaat tgttcagaac tagcacgtaa tgatgtaata gcttctactg
     1141 gatattggta aagtttacca tcttttattg aaagttcttt tacaagactc attgctccct
     1201 gataatcata cttatcagtt ggataatcga tatcaggtag tccaatccaa ctgacaatca
     1261 gagcacgacc atcaggagta ttgaatcctt gagtcgcata cgcttcaaat ccataatcaa
     1321 gattttgaat ttctgaagcg cccacaagtt ttactttttc tatgtcaagc gattgacaga
     1381 tcttaaaagt attcggatag atattcccat aatttagctc agatttatcc aacccttgag
     1441 gacaataaat gagaacaggt tttttgtcta caaatactag atttggacac tcaatcatgt
     1501 attctgaacc agtgccgcca aaatcaaggt tgccaacttc ctcccatgtt tccacgttat
     1561 tgtcatccgc tttgtaaagt ttgataaagc ctgatttttc taaattttgt gctccaacaa
     1621 tcgcatacaa ttgtccttca taattaaaca tttgtggatc acgaaaatga tcagttacat
     1681 cagcaggttt atgaataaga acattctcac ctttcgtaat gttgtagtct ttatccatcc
     1741 atgctccgat ttgaagtgta tcacgttccc aattttcatt tcgaacgttt cctgtataga
     1801 gtaagaatag attcccctct atttcatatg cagaaccgga ataagcgccg tggctatctc
     1861 gttttgtgtc tggaagaagt tctgctcctg ttacatgaaa atgaactaaa tcatctgatt
     1921 cattgtgtac ccattctttc aacccatgag cagctccaaa tggccaattt tgataaaaaa
     1981 gcgtatattt tccgttaaaa aaagaaaaac cattaggatc gtttaacaat cctgttttag
     2041 gttcgatatg atagctagca cgccaaggtg atcgttttac attatcctta attttatcca
     2101 tctcatcttt agtccaatca gaataagcac gatatcgtat ttctcgaggt aagttcatat
     2161 tttactcctt caatttatct ttttacaact agtatggtaa acgttttccg ttgaaaaatc
     2221 aatcatttca tttaaaaaaa taaactatat gacaaacgtt tgacatattt ttaagatatg
     2281 ctaaaataaa tttgcatttc agtgaaccgt tttcacgtaa aaataaaaaa ggagtgattc
     2341 gaatgaatta cagtaaagta gcttccgacg taatacaagc ggtcggaaaa gataacctta
     2401 ttgcagctgc acactgcgct acacgtcttc gtctcgtact aaaagatgac acgaaagtga
     2461 atcaaaaagc tttagacgaa aatccagatg tgaaaggcac atttaaaatt gatggacaat
     2521 atcaagtgat tatcggtgca ggtgatgtta atttcgttta tgacgaactt atcaaaaaaa
     2581 ctggactttc ggaaatttca acagatgatt taaaacaaat tgctagtaaa gataaaaaat
     2641 tcaaccccat tatggcatta attaaattat tatcagatat ttttgttcca attatacctg
     2701 cccttgttgc aggtggttta ttaatggcat tacaaaactt tttaacagct gctggacttt
     2761 ttggaccaaa atcaattgaa gaaatgtatc ctgcgattga aggtatttcc tcaatgatcc
     2821 aactgatgtc cgctgcacca ttcatgttct taccaatttt agtaggtatt tcagctgcta
     2881 aacgttttgg agcaaaccaa tttcttggtg ctgcaattgg tatgatcatg acaacaccag
     2941 atcttggtgg ctcaactgaa tattggaata tttttggtta tcatgtttca caaacaaact
     3001 atgcttatca agttattcca gttttagcag ctgtttggat attatctata ttagaaaaat
     3061 tctttcataa aaaattgcca tcatctattg actttacatt cacgccatta ttatcagtaa
     3121 tgattactgg atttttgact ttcacagtta ttggccctgt aatgttattg ttgtctaatg
     3181 gaattacaga cattattgtt tggctatata acactactgg atttattgga atgggtattt
     3241 ttggaggaac atattcactt atagtcatga ctggtttaca tcaatcattt ccagctattg
     3301 aaactcaatt actttcagcg tggacaaatg gtatcggaca tggagacttt atctttgtag
     3361 ttgcatctat ggcaaacgtt gctcaaggtg ctgcaacatt tgccatttgg ttcttaacta
     3421 aaaattctaa gacaaaaagt cttgcttcct ctgcgggtgt ctcagcactt cttggaatta
     3481 cagagcctgc cttgttcgga gttaacttaa aataccgctt tccattcttt tgtgctttaa
     3541 ttggttcagg aattgctaca gcagtagcag gattacttaa agtagttgct gtttcacttg
     3601 gttcagcagg tttcttggga tttctttcaa ttaatgcaac atctattcca ttttacgtat
     3661 tgtgtgaatt gattagcttt gtagttgctt ttgcaattac ttacttctac ggtaaaacaa
     3721 aagctgctgg tatctttaaa gcagaagaaa ttgctgaaca aacaccagtt aattcttcaa
     3781 aagtagcaac aacagatcca atatctactg atgttgaatc aacacaaaca aaagtttcat
     3841 cagaaacaat tgttagtccc atttctggta aagtgattag tctttcttca gtaaatgatc
     3901 cagtcttttc atcagaagct atgggaaaag ggattgccat taaaccaaat ggaaacacgg
     3961 tccattctcc agtagatggt actattcaac tcacgtttga aactggacat gcttatggta
     4021 ttaaatcaaa taatggtgct gaagttctaa ttcatattgg tattgatact gtatcaatgg
     4081 gaggaaaagg atttacacaa aaagttacta ctaatcaaaa agttagaaaa ggcgatattc
     4141 ttggagcatt tgaccgttca atgattgaga aagaaggtct tgatgataca acaatgatta
     4201 ttgtgaccaa tacttcggat tattcagaag taaatatagt agctgaaaat acagtcactg
     4261 agggtactga tttacttgaa atcaaataaa tatatttccc tgagtaaaga tggaattttc
     4321 tttccaaaat caaataatat agattaatca aatatttgag aataacattt tatttaatct
     4381 tttcatatat caattgtatg aaaaattatt agaattttaa aaa
//
LOCUS       DASJSZ010000141         4409 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.147, whole genome shotgun sequence.
ACCESSION   DASJSZ010000141 DASJSZ010000000
VERSION     DASJSZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.147"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(718..3867)
                     /locus_tag="U4B03_002715"
     CDS             complement(718..3867)
                     /locus_tag="U4B03_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEN2069510.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            4278..>4409
                     /locus_tag="U4B03_002716"
     CDS             4278..>4409
                     /locus_tag="U4B03_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2069511.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT         1224 a          900 c          732 g         1553 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccctcc ctcgtaaaat atcatttgtc
     3901 taatagtttt tattcaaaat gtttctagat tataaaattt attgaataac acattcctaa
     3961 cggaatttgt ttcgatattg tgtgatttct cagagtacaa tggatgtatg gaaaggatcc
     4021 aacgggaatt tgctatttcc tcctgtagcg tttgtctacc ctgtttagtc tgttacccga
     4081 accactgtta tcactaccaa ctcattagct gtttcgataa tggactagac tttcaaatag
     4141 tagtttggaa ggcagcttct agctctttta tcaccgtatt ctattaggcg aggttgtggc
     4201 tatacagtga gtttctggga aatcccacag tatcgaagca ttttccaaaa tttatatcga
     4261 aaggaggtcc ttcttccatg aaattatttg tcggtattga cgttagctct gaaaaactag
     4321 atgtttgttt tttaacagac gacaatcaat tgtctatctt atctgaaatc tctgttgcca
     4381 atgacatcga aggtgcttca ttcatacga
//
LOCUS       DASJSZ010000142         4402 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.148, whole genome shotgun sequence.
ACCESSION   DASJSZ010000142 DASJSZ010000000
VERSION     DASJSZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.148"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            152..1075
                     /locus_tag="U4B03_002717"
     CDS             152..1075
                     /locus_tag="U4B03_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN2069512.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1081..2334
                     /locus_tag="U4B03_002718"
     CDS             1081..2334
                     /locus_tag="U4B03_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN2069513.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2468..3814
                     /gene="gor"
                     /locus_tag="U4B03_002719"
     CDS             2468..3814
                     /gene="gor"
                     /locus_tag="U4B03_002719"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEN2069514.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1516 a          699 c          945 g         1242 t
ORIGIN      
        1 tcacaatctc tttttatatt tggacagaat atatcgaact ttattttgat tttttgtgaa
       61 ataattttca aaaatccgac tttaaataga agaagtattg gaaagttttt agagctatgc
      121 tactattatg aagttgtagg aggagaaaat tatgatcgaa ctcataacca cagtagaaac
      181 tattgaacaa gcagaacagt tgcttgctgc aggtgcagat acactatatt tcggtgaaga
      241 gcaatttgga ttgcgtctgc ctgcttcatt tacacgagaa gaacagcgac aattggtaga
      301 attagcacac aagtatggta aaaaagcgaa tattgctgta aatgggatca ttcatcctga
      361 aaaaatgaaa aatgtacctg aatatttggc ttttttaaaa gaaataaacg tagatgtgat
      421 cacagttggt gatacaggta ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc
      481 gtatatttat gatgctgaaa cattagtaac gagcgcacgt caaatcaatt tctgggcgaa
      541 aaaaggcgca gttggagcag tacttgctcg tgaagttcct tttgaagaga tgaaggcgat
      601 ggaagaaaaa ctcgatattc ctgtagaaac ccttgtgtat ggtgctacat gcatccatca
      661 atccaaacgt ccactgctac agaattacta taactatacg aaacaagacg aacaaaaaga
      721 tcgtgaaaga ggtcttttta tctcggaacc taaaaaagaa gagacacatt attctattta
      781 tgaagatagt catggcacac atatttttgc cagcaatgat ctgaatctct cgaatgaatt
      841 gaatgaactt tatcagcatc attactgtac atggaaattg gacggattat ttactccagg
      901 cgaaaaattc gtaaacatca ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg
      961 tagctgggat caagaaaaag cggaaaaaat gactagcgaa atcagtaaat tgcatcctga
     1021 aaaccgtgaa ttagatacag gattctttta tattgatcct tctgctatca aataaggagt
     1081 atgatgaaag aaatgacagt aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca
     1141 ggaacactgg aaaagttaaa aaccgcgatc cattatggag cagatgcagt atatattggc
     1201 gggaatgcat atggtttaag aagccgtgca ggtaatttta cttatgaaga aatggctgaa
     1261 ggagttgctt ttgcaaaaga acatgatgcg aaggtatacg ttgctgctaa tatggtcacc
     1321 catgaaggaa atgaagatgg agcaggggaa tttttccgca gtttgcgtga tgtcggtatt
     1381 tctgcagtga ttgtttctga tccagctttg atcgaaattt gtgcgacaga agccccaggt
     1441 ctgccgatcc atttatccac tcaagcttca gcaactaatt ttgagacatt ggaattctgg
     1501 aaagaagaag ggttggaacg tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca
     1561 gagattcgta aaaatacgga taccgaaatc gaagcattta tccatggtgc catgtgtatc
     1621 tcttattctg gtcgatgcac attgtccaac catatgtcga tgcgggatgc aaaccgtggc
     1681 ggatgttctc aatcttgtcg ctggaagtat ggattgttcg atatgccttt tggtcaagaa
     1741 cgaaacagtt taactgatac aggcgaagtg gaagaagaat tctcgatgag tgcagtggat
     1801 atgtcaatga tcgaacatat tccagaattg attgaaaacg gcgtagacag cttcaagatc
     1861 gaaggaagaa tgaaatcgat ccattatgta tctacggtgt caaatgtcta taaagcggct
     1921 gtagacagtt atatggaaga tcctgaaaac tatgtctgca agcaagaatg gatcgacgag
     1981 ttatggaaag tagcacaacg agaattagct acaggttttt actacaatac accaactgaa
     2041 aacgaacaat tattcggtga acgtcgtaaa atcccagtat ataaatttgt cggtgaagta
     2101 ttatcttatg atgaggcaac acagattgct acgatacgtc agagaaactt gttttctgta
     2161 ggagatgaaa tcgaatttta tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt
     2221 atgcataatg aagaaggcga atccattgac cgagcaccaa atccaatgat gattttaact
     2281 atgccagtcg cacagccggt gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa
     2341 agctgatata tccctgtttt aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact
     2401 tgttttctgt agaaaaagga tcgttaaata aaggttttga ttgatctctt caataaggag
     2461 gaacaaaatg caaatatatg attacatcgt aattggtggc ggctctggcg gtattgcttc
     2521 agcaaacaga gcagggatgc atggcgcaaa agttttgctg attgaagcga atgaattggg
     2581 cggaacatgt gtcaacgtcg gttgtgtacc taaaaaagtt atgtggcaag caagtacgat
     2641 tttggaaaca attgaacgag atgcctcaag ttacggtatc caagcagatt taaaaaatgt
     2701 tgatttcgcc ggtctagtag aaagacgaga aaaatatatt gattttctac acggtgctta
     2761 tcaaagagga ttagatagca atcatgtcga ggctataaaa ggttacgcta cttttatcga
     2821 tagtcaaacg atcgaagtaa acggtgaaac ctatcgtgct cctaaaatct tgatcgctac
     2881 tggaggccgt gcaagtaaaa tgaccattcc tggtggagaa tacgcaattg attcaaatgg
     2941 attttttgaa ttaacagagt tacctaagag agcaatcgtt ttaggtgctg gatacattgc
     3001 ggctgaactt tcaggtgtat ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa
     3061 agaacgacca cttagaacgt ttgataaaat gctttcggac aatttgatcc aaatgtacca
     3121 agaagctgga ataaaaactt acgcacatca tattgctaaa gaaattacta aaaacaatga
     3181 cgaatatacg gtgctatttg aaaatggtga aaccttaact ggtgattgtg tactttttgc
     3241 cggtggacgt gtaccgaata ccgaaaaatt aggtctggaa aatacaaatg tggaattgga
     3301 caaaaaaggg tttatcaaag tagataaata ccaaaataca acggatgaac atatttttgc
     3361 tgttggtgat gtgatcggta aattagatct gactcctgtt gcaatcgcag ctggtagacg
     3421 tttatcagaa cgtttattta atggaaagaa agattcttat cttgactata aacttgttcc
     3481 gacagtcgtc tttacgcatc caccaattgc aacaattggt ttgacagaag aagaagcatt
     3541 ggaaaaatat ggcgaaaatg aattgaaagt ttatcgttca cgctttaccc caatgtactt
     3601 tgcattaaat gattatcgcc aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga
     3661 aagaatcgtc ggcttacatg ctattggtat tggtgtcgat gagatgctgc aaggttttgc
     3721 cgtggctatc aagatgggtg caactaaaga agactttgac aatacagtag ccatccatcc
     3781 aacaggagcg gaagaattcg taacgatgcg ataatttaat attaacagtt ccaacagctt
     3841 tttattgaac aaaccatgta aaaccagaaa atcagtatac tgattttctg gttttttgtt
     3901 ttttagttat ttatgtaaga ttgcttcgat aattctcaaa acgaaataag tagggaagtt
     3961 ggagattttg attatcttcg taactgacga agagtaaatg aatccatttg gtatgcactc
     4021 ttaagttcaa aatattggta tcgatgttta aaagaaatag aaatgtatcg aagaacagat
     4081 gtttaaaaaa gtgtgacttt gtataaataa attagaagaa gcaaacgagg atgttaaaag
     4141 aataccatat agttaccaaa ccaagaaagt tctcgtcaag ttcgctggta cgaatcaaat
     4201 caagtgaaaa aagggactgt gaaaaaaatt gtgtcatagt tagtcacttt ttccgaacaa
     4261 atagtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact
     4321 atttttccat ttgtttctct aatactcagg acaaaacact gattttacaa cctctgataa
     4381 acggtgttca aaagccagtt tc
//
LOCUS       DASJSZ010000143         4336 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.149, whole genome shotgun sequence.
ACCESSION   DASJSZ010000143 DASJSZ010000000
VERSION     DASJSZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.149"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..579
                     /locus_tag="U4B03_002720"
     CDS             127..579
                     /locus_tag="U4B03_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069515.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="U4B03_002721"
     CDS             593..1282
                     /locus_tag="U4B03_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069516.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="U4B03_002722"
     CDS             1407..3758
                     /locus_tag="U4B03_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEN2069517.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="U4B03_002723"
     CDS             3838..4233
                     /locus_tag="U4B03_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069518.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1395 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttattt
//
LOCUS       DASJSZ010000144         4248 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.150, whole genome shotgun sequence.
ACCESSION   DASJSZ010000144 DASJSZ010000000
VERSION     DASJSZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.150"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..486)
                     /locus_tag="U4B03_002724"
     CDS             complement(1..486)
                     /locus_tag="U4B03_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEN2069519.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(488..1252)
                     /locus_tag="U4B03_002725"
                     /pseudo
     CDS             complement(488..1252)
                     /locus_tag="U4B03_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1422..2615)
                     /locus_tag="U4B03_002726"
     CDS             complement(1422..2615)
                     /locus_tag="U4B03_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2069520.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(2699..2899)
                     /locus_tag="U4B03_002727"
     CDS             complement(2699..2899)
                     /locus_tag="U4B03_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN2069521.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3323..3556)
                     /locus_tag="U4B03_002728"
     CDS             complement(3323..3556)
                     /locus_tag="U4B03_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069522.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(3563..3994)
                     /locus_tag="U4B03_002729"
     CDS             complement(3563..3994)
                     /locus_tag="U4B03_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2069523.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1045 a         1061 c          827 g         1315 t
ORIGIN      
        1 ttatttacct tttttgtatg ctgcactgtc aatccaagtt cctaaattgt tgtatccatc
       61 atcacttata taatcatatt ggccatcaat aatttcgtca aaccacaaat attctccatc
      121 tggtttttgc ccaattattt taattactga ttgactagta gttgtcatct ggttaaaatc
      181 ttttatttta cctatattaa cggccaaaat agcatttcct cgcttataac tctcctggat
      241 ttcatattta acatattctc gatcaagagt ttctttacct atcagcacca cagtcactga
      301 tgttccaatt aattgattat ctatccagtt ttttatagca atatctccat tacgtttaac
      361 tttctcaaat tcagccttat ctacaaaacc agcttccttt gttcctcgca aagtccagct
      421 gtttctaact accattgctc ttgttacatc tgcataatgg aaactaaaaa aaacccttct
      481 caccatatta atcctcctga atactattta ttaaactgac aatttgttca acttttttat
      541 ctacttctcc ttcagtatct aatttttcta gcgcttgaaa tttacttttt aaggaatcat
      601 caaaataatc actgaaattc tcttgaatct tatcaaacaa aaccttactt gcacctttgg
      661 taaatccaat cggaactact atattttcat tttgaacaga taattcaaat tcttcaatca
      721 ttccatctgc tgtaactatt tcacctgatt caagtttgtt tccaaaaaca aaaatactaa
      781 tcccagactc tgcaataatg ttttctcgat atcttctcca ctcatgaggc atatcaaggg
      841 gaagtggtct taaagaaagt acgtcttgta tgttattttt tcttaaattt cttctctgct
      901 ttagaactcc tgaaacaata ctattaccaa ttccaagtcc gtaacctgat actattctat
      961 aaccggattc agaaagccta tatgctaatt tctcgacaaa ttttaatcct ttcgattcag
     1021 atgtaaatgg agtataatca acagcacttc ccgaaataaa aatagttttt ctcaaatact
     1081 tttttctaat acttttcaaa atatcagtaa tctcagaata atcatctact tcaacaacgt
     1141 ttatcgaaaa tctggacaaa tcattgattt gtagtcttct ttttgtcagt tcataattat
     1201 atctctcttg atcaaaaaaa tcctcagaat cgacaaaatt ttcctttaaa attatatggg
     1261 attttagatt atctttttag aagttcttga atgtcggtat agcccgtgtt tccgggcttt
     1321 tccggcattt ttgatattgt tagatactta caaatgttct tattacctct caccatttcc
     1381 ccctcaaaag tagtaaatgg agtagtaaaa ccggccttgt gtcaggctgc cagccgttcc
     1441 atttctgccc ttgcggaatc gaatgtggcg tgtgcataat agttcagcgt catggtgata
     1501 ttggaatgtc ccataatgta ctgcaaggct ttggggttca ttcccgcatt ggctaaccgg
     1561 gtgcagaatg tatgacgcat agcatggggc gtcgtcgttt tcggcaacgc ttcttcatgg
     1621 ctcttgttgt acttcttcac aagcctgcca aacatcccgc cataatctac cgccgtcatg
     1681 ggatagccgt tctgtttgag aaacaggaaa ttggcatacc cgtcaataat gaacggcttt
     1741 gcacctttgc ggttttttat cactcgctgg aacgcctgat acacttcctc attcatgggg
     1801 atttttctga cgccgctttg ggtcttgggt tcgtcaatat agtaaccgac gccggtattt
     1861 ctcaaaagct ggtggctcac gatgattacc cggttttcaa aatccaaatc tttgtccgtc
     1921 agtccgcaca gttccgaaat gcgaagccct gtccccagca gaataacgat tgcgtcataa
     1981 tgcttttggt aaaccttatc accttgaata aaggacagaa aactttcttc ctgtgctggt
     2041 gtaagaggca cctttggttc cgtatcgtcg tccagaacgt cactaagctg gaagtcaaac
     2101 gggttttttc ggatgcaatc gtcctgtatg gcggtgtaaa aagccgcttt cagggaacgc
     2161 ttgtcgttat tgatggtctt ataagaaagg cccttttctt tcatccgaag cgcccattcc
     2221 tttgcgtcgg acagcttcac actgtcaatc gggcagctcc ccagccgatc ttgttccaga
     2281 atacgcatca gccgttcccg gcccagtttg gtgttgtgct tcacattgcc ccggtgcctg
     2341 atctgcttgg cgtagagctg gcagacggtc attttcttgc cgatggtgtc tatcccgtcg
     2401 tcaaggtctt tctggatttc tttctccttt tcccggagtg aaatatcctc ccgtttacct
     2461 gccggggtct tatccgtagg cactaacttc catgagtaaa caaactgcgg ttttccaaag
     2521 gtatcaacat atttgtaagc atatcgtccg tcttttctct ggctctctcc agaccgcaaa
     2581 acgcgatttt tgctgtctcg tcttttttct gacatctcgt catgctcctt tccatgacgg
     2641 aaagagccgc atatatgctt ataaattatt ataacatatc tgcggccctt tttcactatt
     2701 agattgtgtc caaagtgtca attattttct caaactgctt gcgtttgatc tggatgcggt
     2761 tgccattcat aatgacccag ccagaggccg gattttcctc ggccagctta cgcaatttgt
     2821 tttctccgat acggaaatat ttagacgctt cctcaatcgt cagcgtgtat ttttcccaaa
     2881 taggcacatc atggttcata atgcagcccc ctttcacaaa ttcaaaggtt tgtgtgattt
     2941 aggggcatag acggacggct gctggcacag ctccacggga ctttcacccc cgcgtggttc
     3001 tcacgcggcc ctacccattg cctgcgacgc ttttaacgct cggactgtgg ctataaggga
     3061 gtatcatagt cctgcctgta cgtcgtcgcc cgcaagccgc cacgctcaca ttgcggcgtg
     3121 gtgttcctcg ctccgctttt cccgtaggga aaagtatcat ggcgcaccag ccgtccggct
     3181 cggcacagac agaatgtgtc cgtatgccct atgatgcgcc gccgttatct tgcgggcata
     3241 tttatcaagg tgcgatcagg ccaccgggaa aacaccgaaa cggggaaagg gtaagccgtt
     3301 tcatgctgcc ggggccggtt cgtcagttcg tgacgatagc gtggatgagc ttgatttcca
     3361 gacggcgctt catggcctcg tccagacaca catgcggata gccgttttcg tcatagagcg
     3421 tccgggtaca cagcttgttc atgtaaccgc tgtaatactg caaaacgcgc tctacggctc
     3481 cggcatcccc agcgcgggcg gcctcgatca ccgaaagggg caaaagctca atgcccttgt
     3541 agttcgggtt cttcatccgc gtctaacctc ccttcggcat caacgccttt agctgtttcc
     3601 gcagctccga taaggtattt gtcctgtgcc gctgaacggc actccgggac attcccacaa
     3661 ggccgccgat ctcaccatct gccatatcca gaacacagtg caggattaaa atactctgct
     3721 ccatgctggg cagcgcggca aaggcttcgg caacaagctc attgttgata tgtaggtcat
     3781 agccatgcga cgaaaacgta aagctgtcgc tggggtagtg atctactgta cagagcttgt
     3841 ccaaatctgc ctgtgacaaa gagcttagtg acacttcacg gtcacggcgg cgcttcattt
     3901 cagccaagta gcttttggcc tcgttacgca gtaccgtctt gcaaaaagca tcgaacatgc
     3961 accgcgcttc atagtcgcgg gggatagcat ccatcttctc accccctttc tgtgggggac
     4021 ttaggtggtt ctctcccttt ccgctaacaa gactttgaca aggccgccag ctacccgcta
     4081 aatacctcct ttttgaaaat ttctcaaaaa atttttttgg ggacagccgg gccgcaaaag
     4141 acgacaaaaa tcgcccggca ggtcgcaaac ctaccgggcg agaaaatgct ttgctatgct
     4201 gctgatttac atggtatagt acatactcat aaccggaata gccctgtg
//
LOCUS       DASJSZ010000145         4069 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.151, whole genome shotgun sequence.
ACCESSION   DASJSZ010000145 DASJSZ010000000
VERSION     DASJSZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.151"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1157)
                     /locus_tag="U4B03_002730"
     CDS             complement(<1..1157)
                     /locus_tag="U4B03_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2069524.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="U4B03_002731"
     CDS             complement(1160..3409)
                     /locus_tag="U4B03_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN2069525.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3506..3961)
                     /locus_tag="U4B03_002732"
                     /pseudo
     CDS             complement(3506..3961)
                     /locus_tag="U4B03_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         1312 a          744 c          580 g         1433 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacgctc acataaatag taagcgttta aagtaacgaa
     3481 tatttatcaa aatcaaatgt ttagtttatt caccattaat gattcctaga cctaatctac
     3541 gttttcgata tactcccggt gaaacaccat ttgctttctt aaaggcttta gaaaaagatt
     3601 gtactgttga gaatcctatc ttgtcactga tttgctggat tgaatattca gttgatgata
     3661 acagatcgtt tgcaatagaa attttgattt cactaatata ctcttttatt gatattccca
     3721 tatcattctt aaacaatcgt gataaataat tttcgtgtat cgatagactg acagcaatat
     3781 ctttaacaga taatttttga tacgcattct cacgtattat ttcaattgtt tgacgaacaa
     3841 aaggattcat ctttttagta attatccctt cgttcagata actagctgaa tttaagagga
     3901 gttctaaaaa tttataaact tgtttttgtc tggacaattc actgactaga ttgtcctgat
     3961 ctttcagctg agtgttaggt tctgttacta aatatataga attttccata tccaccctcc
     4021 tatgttagat ttggataatt tttgttttat tatgtttata ttattacat
//
LOCUS       DASJSZ010000146         4064 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.152, whole genome shotgun sequence.
ACCESSION   DASJSZ010000146 DASJSZ010000000
VERSION     DASJSZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.152"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(173..1678)
                     /locus_tag="U4B03_002733"
     CDS             complement(173..1678)
                     /locus_tag="U4B03_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2069526.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1675..2361)
                     /locus_tag="U4B03_002734"
     CDS             complement(1675..2361)
                     /locus_tag="U4B03_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2069527.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2554..3975)
                     /gene="gndA"
                     /locus_tag="U4B03_002735"
     CDS             complement(2554..3975)
                     /gene="gndA"
                     /locus_tag="U4B03_002735"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEN2069528.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          858 c          694 g         1396 t
ORIGIN      
        1 cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg gatgttattc
       61 tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac ttgcttgtat
      121 tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg ctttacttgt
      181 cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct actacacttt
      241 ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac aaccccagac
      301 catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga ttgaagatct
      361 tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc tcaaattcat
      421 tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac tttactgcgt
      481 tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc agggtcactt
      541 cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc ttgaaattat
      601 tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa tgaacatcta
      661 cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata cggctgattt
      721 cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc ccccaacgat
      781 ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca tgagatacgt
      841 cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc atctcattga
      901 atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg tggatatctg
      961 attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt ggttttaaga
     1021 aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca acttctaaaa
     1081 caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat tcatagaagc
     1141 tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg atagataaaa
     1201 agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt
     1261 ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa acataaagat
     1321 ccattgatgg ctgccctagc tcagctataa agggatcgat cttcattaag tttccttcga
     1381 ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc agattacgat
     1441 acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct acttctgaaa
     1501 tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat gatttatagg
     1561 taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca aaggcccact
     1621 tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc tttatcacga
     1681 gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc cgggaacgtc
     1741 aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa cttctgtttc
     1801 gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca cgttttccat
     1861 taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat ttccgcgacg
     1921 tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag tttgtttagc
     1981 cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac gtgctaataa
     2041 ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa gtccagatac
     2101 acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt tgacttggcg
     2161 tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca acaatatggc
     2221 atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt ggacttctgc
     2281 gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct tttcatcttc
     2341 aatgattaga atattgctca tttattattt tatcctttct attctactgg ctttttaaaa
     2401 actctatcat tataccgata atatgcttaa aaatctatag tcattttatc atggatagca
     2461 attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt cgtcctcttt
     2521 ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc atgagtaatg
     2581 gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc
     2641 ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag cgatggctga
     2701 tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt ctctgactga
     2761 ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat tttcgatctc
     2821 aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc
     2881 agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc
     2941 gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat acaacgcttc
     3001 tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga
     3061 caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc gggcaaatac
     3121 agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc ttgtccattt
     3181 acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt tacctgttcc
     3241 ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc tgtctaattc
     3301 gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt ttaaaatatt
     3361 cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa taccgttgtg
     3421 taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac atggtgctcc
     3481 gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat aggcttcttt
     3541 ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac cggaaacgcc
     3601 tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca tcgtatcttt
     3661 aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag gaagtaactc
     3721 ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc gacgtggttt
     3781 ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt tatctgggtg
     3841 ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca ctgaatagcc
     3901 gcggctttct atgttcaagg ctagattttt acccataacg gccataccga caacgccgat
     3961 ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat ctcttacatt
     4021 atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DASJSZ010000147         3942 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.153, whole genome shotgun sequence.
ACCESSION   DASJSZ010000147 DASJSZ010000000
VERSION     DASJSZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.153"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            85..1434
                     /locus_tag="U4B03_002736"
     CDS             85..1434
                     /locus_tag="U4B03_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2069529.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="U4B03_002737"
     CDS             complement(1562..2152)
                     /locus_tag="U4B03_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN2069530.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="U4B03_002738"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="U4B03_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN2069531.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1125 a          727 c          773 g         1317 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg ca
//
LOCUS       DASJSZ010000148         3845 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.154, whole genome shotgun sequence.
ACCESSION   DASJSZ010000148 DASJSZ010000000
VERSION     DASJSZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.154"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="U4B03_002739"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="U4B03_002739"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEN2069532.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="U4B03_002740"
     CDS             complement(2260..2511)
                     /locus_tag="U4B03_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEN2069533.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="U4B03_002741"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="U4B03_002741"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEN2069534.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="U4B03_002742"
     CDS             3461..3679
                     /locus_tag="U4B03_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069535.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1064 a          816 c          714 g         1251 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctt
//
LOCUS       DASJSZ010000149         3840 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.155, whole genome shotgun sequence.
ACCESSION   DASJSZ010000149 DASJSZ010000000
VERSION     DASJSZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.155"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(220..960)
                     /gene="truA"
                     /locus_tag="U4B03_002743"
     CDS             complement(220..960)
                     /gene="truA"
                     /locus_tag="U4B03_002743"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN2069536.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1151..1786)
                     /locus_tag="U4B03_002744"
     CDS             complement(1151..1786)
                     /locus_tag="U4B03_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN2069537.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(1933..3174)
                     /gene="sstT"
                     /locus_tag="U4B03_002745"
     CDS             complement(1933..3174)
                     /gene="sstT"
                     /locus_tag="U4B03_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEN2069538.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3372..>3840
                     /locus_tag="U4B03_002746"
     CDS             3372..>3840
                     /locus_tag="U4B03_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069539.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNK"
BASE COUNT         1270 a          789 c          586 g         1195 t
ORIGIN      
        1 attttatttt tactttatcg atttactttt tctaaacaaa aacaaccgag aagagaagta
       61 caacattctc actagatttt tatttaagaa agacttttta gctgtacaca taaacaggct
      121 aaaaagtctt tttttatatt tctttaattt gcttcataca ttatttaaca tcgatattta
      181 tactgtgaaa catgcgtttc atccattatc aaagtatttt tatcgataat aaacttcatc
      241 caaaaacaga ccttgtgcag ggacggtttc tcctgcatgt tgccgcactt tgctttcaaa
      301 tatttcatcg attgtttcta tcggcatggc acctgtgccg atttctagta atgtccccat
      361 gataatccgc accattttat ataagaatcc gtctccgaca aaagtaaaat gaagcatttc
      421 tccctttcgt tcgatagata attcatcaat cgtacgcact gtggattttt ttgatttttt
      481 caatgcagaa aaaccaatga aatcatgtgt acccactaat ttttcgcaag ctgcattcag
      541 cttgtccata tccaactctt gagggaaata aaagctgtgg tttcgctcaa atgctgaagg
      601 aatcacgttg ttccaaacat aatagctgta ttttttgccc accgcattat atcttgaatg
      661 gaagcgctca ggcatttctt cgattctttt gatgacaata tcccgcggaa ggtaacgatt
      721 cataaagtcg atcatttctg ccagtccaat ttctgtgttt gttttaaaat ttgctacttg
      781 tcctcgcgca tgggtgccag catctgttct tcctgatcca ataatttcta tctcttcatt
      841 tgtcatctga tgcaatactt tttcgatttt tccttgaatg gttttatcgt catctccaag
      901 tctttgccat cctgaatatc tttttccgtc atattcgatt gtcaacttga tgtttctcat
      961 attttagtgg ctcctctgtc ttccatttgg tcttctctct acctttttaa gattgtagca
     1021 caaaatatag ctccttgctt ctctaagaca gaagctatac aagaaaactg tggaaatata
     1081 tatttcagaa aaacaaaaaa cagccaccgg atgaagtatc cgctgactgt tcaatttgac
     1141 ggctcaagtt ttactgtttg agatgcaaga tactcaacca ggcaatgggc acataattaa
     1201 agaaaaaact gattctttgg caaaaaccta ttttattcag taatggccaa gaatttatag
     1261 aaggaattct tgccagtcca taaatcgccg caaaaagcaa actgatcaca gacagtccaa
     1321 ggtataagtg gcttacgtcc atttgcccat tttttcgata gatcaagaaa agaagaactg
     1381 gaaacaataa aaaaccactg taacctaaac ctgatccgat attatggacc catgtcgaga
     1441 cattccacga agattcatgg gtattgatac tgaacaaacc tgtaaagata caatcaccta
     1501 taccatagaa cgcgatggct gctgtaacta gggtagctaa cacacgagat gtactaacaa
     1561 atgcagcata caatgcaggc aatgacagga tgaaaaagat ccctgaaacc acagaccaaa
     1621 taagaaacgc ttttcttaca ggactggaaa cctctccaaa tacactgatc acgctcttca
     1681 tttgatctaa ttcaggataa tacaatccta atacgtacgg agtcaaaaaa tcactcagca
     1741 cgcctaataa taaaaagaaa aaaccaaatt tacgtaaaaa atccatgata cgcctccaat
     1801 aacaattacc tatgctttta attgttatta taaacgactt tatcttgatt taacagaaac
     1861 ctctatttac acgaacatct atataactca ttaaaaactc gaatcatttt aaaaatacta
     1921 aatatctaac gttcaacgtt cacttaatcc atctaaacaa tctttcaatt tgattttttc
     1981 cccacttctt ttacgcgcgc ctagctctcc tattgcagta aacaacagat cacttgacga
     2041 atttaaggca gtttccattg aatcctggat aacaccgata ataaatccaa taccgacgat
     2101 ttgcatcgcg acatcattag agataccaaa caaactacat gctaatggaa tcaataacag
     2161 tgaaccgccc gcaacacctg aagctccaca agcagctaaa gcagacaaaa tacataaaag
     2221 gactttcaaa acaaaaggtg cttcaatccc taaagaatgt acagcagtca acgtcatcaa
     2281 ggaaatggta atcgcggcac cgcccatatt gatcgtagca cctaacggga tcgatatgga
     2341 ataagactct tttgtcaatt tcaatctttc agccaacatc atgtttatcg ggatatttgc
     2401 ggcagaactt cgggtaaaga aagcgggaag tgcgctttct ttcaaacaaa agaaaatcaa
     2461 tggataggga ttttctttaa taaacaaaaa cgtgaggaat gggtacacaa ctaatgcgac
     2521 aaacagcatc gtaccgatca aaacaagtaa aagctgcaga tactctgcca atccagcgat
     2581 tccagtcgta gcaattgatt gataaaccaa tcctaaaatc cctatcggag caaatgcaat
     2641 aatcacttgt acgacttgtg acaacgctat agatatctga tccaccaact cttttgtcga
     2701 ttctgaacgt gctcgaaaag ctagtcccaa caatacagcc cagaataaga cgccaaggta
     2761 attcgcatca ataagagatt gaacaggatt ttgtgtgaca ttcgtcaaca tagtagataa
     2821 aacactttcc aaactttgcg gtgctttttc agatactgtt tcttgtaaag taataggaac
     2881 agtaaaaatt gaacttacaa taactgctac aagtgcagaa agcaatgttc ccactaaata
     2941 taagatcaaa ataggcttca cgaatgtctt attcccaatt ttatgcttgg caattgacgc
     3001 catcgtcaag aaaaaaacca gaataggtgc gattgccttc aagcaactga caaacaacgt
     3061 tcccaatatt gaaataaatg tccatgtggg gaaaaagata ccaaacaata ttccgactaa
     3121 aataccaaca acaatacgtt gaactaacga catatccaaa aacttctcta acaaatgacc
     3181 accctttcac aattttaaca atcattgtat cattttatgc aaagaaacta aaggtataat
     3241 tttctaaaaa ttctaacaat acaaacaacc atttcttgct aattttgtac tttttctgtc
     3301 tacacgttgt tttttgagat attctaagtt aggatgttat taatgggcaa cttatcagga
     3361 aacgttttct tatgtttttg tccaattttt ttgtgaggtg tttattcttg aaaaaaataa
     3421 agaaggagag ttatggcttc ctgacaatct ttgtcgcttg tataatgatg gttttacagc
     3481 gtatattgat ttcagcagtc actattccta catatgcaac aaaccctggt cgattctaca
     3541 tctatacatt tttacaaact tccgttgtta ttggtgcaaa tttgatccca ctttacatgg
     3601 gttaccaata tcaaaaaatc gcccgtttaa aagttttaca ttatttaagt cgttttgcct
     3661 ttatattcct aatagccaca ctagtttgca atatcttttt ttacgtacaa gctggcagct
     3721 tgaatattcg tgattattgg cttatcctaa cacctatcag tcagaattac tttacttatg
     3781 ctgtttcttg tactttgctt ttttgtagta ttccttatac agttggattt ttaaataaat
//
LOCUS       DASJSZ010000150         3838 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.156, whole genome shotgun sequence.
ACCESSION   DASJSZ010000150 DASJSZ010000000
VERSION     DASJSZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.156"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="U4B03_002747"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="U4B03_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEN2069540.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="U4B03_002748"
     CDS             complement(2066..3064)
                     /locus_tag="U4B03_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEN2069541.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..>3838)
                     /locus_tag="U4B03_002749"
     CDS             complement(3290..>3838)
                     /locus_tag="U4B03_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEN2069542.1"
                     /translation="EIENFFYSILEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNL
                     PDEEISQSEDSTVDSEDVSDFLRQQIIGSDPEEELSGSALEEQERQATFAFEDFEQVI
                     QLATDVELESAWTDLYLFEERYYLVVHFWMENLNQADVENQIARILEFAEKSDRTPET
                     LNEYGQCLMARNAIERTKFYFV"
BASE COUNT         1112 a          815 c          612 g         1299 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttc
//
LOCUS       DASJSZ010000151         3718 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.158, whole genome shotgun sequence.
ACCESSION   DASJSZ010000151 DASJSZ010000000
VERSION     DASJSZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.158"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..353)
                     /locus_tag="U4B03_002750"
     CDS             complement(<1..353)
                     /locus_tag="U4B03_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069543.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="U4B03_002751"
     CDS             complement(613..1161)
                     /locus_tag="U4B03_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069544.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="U4B03_002752"
     CDS             complement(1162..2019)
                     /locus_tag="U4B03_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2069545.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="U4B03_002753"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="U4B03_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="U4B03_002754"
     CDS             complement(2581..2910)
                     /locus_tag="U4B03_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2069546.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="U4B03_002755"
     CDS             complement(2987..3259)
                     /locus_tag="U4B03_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN2069547.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="U4B03_002756"
     CDS             complement(3259..3522)
                     /locus_tag="U4B03_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2069548.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DASJSZ010000152         3717 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.159, whole genome shotgun sequence.
ACCESSION   DASJSZ010000152 DASJSZ010000000
VERSION     DASJSZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.159"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            72..155
                     /locus_tag="U4B03_002757"
     CDS             72..155
                     /locus_tag="U4B03_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEN2069549.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="U4B03_002758"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="U4B03_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN2069550.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="U4B03_002759"
     CDS             complement(1280..2143)
                     /locus_tag="U4B03_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2069551.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="U4B03_002760"
     CDS             complement(2145..2417)
                     /locus_tag="U4B03_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2069552.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="U4B03_002761"
     CDS             complement(2434..2643)
                     /locus_tag="U4B03_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN2069553.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="U4B03_002762"
     CDS             complement(2721..3617)
                     /locus_tag="U4B03_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2069554.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1143 a          652 c          575 g         1347 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttttat
//
LOCUS       DASJSZ010000153         3607 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.160, whole genome shotgun sequence.
ACCESSION   DASJSZ010000153 DASJSZ010000000
VERSION     DASJSZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.160"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(223..900)
                     /gene="nth"
                     /locus_tag="U4B03_002763"
                     /pseudo
     CDS             complement(223..900)
                     /gene="nth"
                     /locus_tag="U4B03_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(907..1605)
                     /locus_tag="U4B03_002764"
     CDS             complement(907..1605)
                     /locus_tag="U4B03_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN2069555.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1873..3057
                     /locus_tag="U4B03_002765"
     CDS             1873..3057
                     /locus_tag="U4B03_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEN2069556.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3063..3506)
                     /locus_tag="U4B03_002766"
     CDS             complement(3063..3506)
                     /locus_tag="U4B03_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2069557.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          934 a          721 c          605 g         1347 t
ORIGIN      
        1 gacttatttt ataggtatat tggaaggcat tattttctac atcgggtatt ttcccaataa
       61 aaataacaac ttttattttt atcgcaataa attgtaataa gttaccatcc tagctactga
      121 gtatgtacat ttactttttc atgcaaaaaa gataaacgaa acagcttttc tccataataa
      181 caagataatt catacattta tttgcaatta aagaaaccac gtctacaggc gtggtttctt
      241 ttttagcgcc ttttctttta gacgcatccg atttttccca tcttgacata ttgatagcaa
      301 aggacatacc tcgcacttgg gatttcttgc cgtgcaatga tatcaaccaa aaaaaatcag
      361 tgtatgatgt gtctttaccc ataattcttg tggtactttc cgcatcagtg tttcttctac
      421 ttccatgacc gtagcatcta gtttgcagat cctcaggcgt tttgagacac gttccacgtg
      481 ggtatctact gcgattgctg gtatcccgaa tgcatccccc aatacgacat ttgctgtctt
      541 tctgcctaca ccaggaagag acatcaattc ttctcttgac gttggcactt gcccatcgaa
      601 acgttcaatc agctgctgtg cacacgcttt gatgtttttt gctttatttc gatacaaacc
      661 aatcgttttt attttaagaa tgatttcatc gattgaagct tctgctaacg catcgggtgt
      721 cggaaaagag gcaaacaaat ctggcgttgc tttattcact gatacatctg tcgcctgggc
      781 acttaagatc actgcaatca ataactcaaa tggattattg tgcttgagtt ctccatgtgc
      841 ttcgggaaac attccataca tcgtttctag agcttccatt gttttttgtt tgcttaacat
      901 attccgctac tcctccaacc aatttttcag tgagactttc ggcaattctt cttgttgagc
      961 tgactgttct acttctcttt gaagaagctg ctgttttctt cgtttttggt cttcttccac
     1021 ttgttgtttt gtacgtagat tttttctttc ccacgatagc aaaatgcggt caacgtaatt
     1081 aaaactataa gcctgattta atacggcttc ccgcagtgct agcttcaaaa tttccggctg
     1141 ataatggtct tcttctagcc attgtccgat ccgctggaac tcgatcgaag aaagcgggcg
     1201 tccaaattct tgttcaaata attgatacac tgactggatt tctttttctt gtgttttttc
     1261 ttcgtatttt ttttcttgtg tttttaaata ctcacctaat agatcataga tggggtaaag
     1321 attatatcga tctgcttttt taccgttgac aatcgtcgtt tctatcttga taaatccatt
     1381 tgttacgaga cgatccagta tttgaaaaat ctgatcttgt ttgattccca tatccatagc
     1441 aatcatctgc aaatcaggga aagtatctcc ttcaagctgt gccatatgca actgtagaac
     1501 aaataaaaat tcttcagatt gtagaccgat cctatgataa tttttaagta aaagattaga
     1561 tatgactgtt tgtccagcgc ttaagtaatt ttctaaattc aacatgattt ctcctccgct
     1621 caatcacagt atacgtaaaa aaaacatggg aagcaagaac aggtttcttt tctatttact
     1681 tcactgatcg gtcagattta caatgtcgtc tacttgattt ttgtttgttc atgtttttga
     1741 tgcgtatctt cacttttcgg aataaataaa ttaagtagat ttcgctgttc cttagaatgt
     1801 tgacaaggaa acaaaattac tatacactat ttttgtttcc aagttaacat tattgcataa
     1861 aggagttttt acatgtacag tttcacaaat aaacattcta aagtttttgg atacgtctct
     1921 gtcttattca ctggattagg atttactatc gtcagtcctg tacttccgtt tttaacgctg
     1981 ccttattctc actccataca tcagcaagct ttctatatca ctttgctcat gtctgtttat
     2041 gctctagctg ccttcttatc tgcccccatt ctcggtagct taagtgatca ttttgggcgt
     2101 catccgatcc ttattatcag tttattagga tcaagtatcg gatatttgct ttttggttta
     2161 ggaaattcga tatggatgtt gttccttggt cgtatcattg aaggattgac ggcaggagaa
     2221 atctccacct tatatgctta ctttgccgac atcacggaac ctaatgaaag aaccaaagtt
     2281 tttggatgga tgggtgcttt agtcggtata ggaacaacgc tgggtcctat tatagggggt
     2341 ctattagctg agttggggaa ttctgttcct atatttattg gagcactctt tactttctta
     2401 aatgccgttt atggctacac atttatgcta gaaagccttc caataaaaaa acgttcagta
     2461 gatttaagtt tctcccatgt aagacctttt caccaattac aacaattatt caagatttca
     2521 tccgttattc ctttattgac agcaggtttt gctgtttggt tggcagctgg ctctcttcaa
     2581 tctatctttt ctcaattttc gattgacgcc tttcagtgga aggctgggtt ggttggttta
     2641 agcttctctt tgattggaat cttggatact gtctcgcaat tgttcattat gcctcgattg
     2701 ctcaaaaaat tttcagaaca gcagatcaca agaataggga tgttcagtga aatcctcgcc
     2761 tatctattca tcacattatc tggaattctt ctactgccga ttttgttttt atttgggatt
     2821 atctgttatg gtttcggtga ttctattttt actcctgtat tcaatggcca actatccaat
     2881 tctgtctcag ataaccagca aggtcttgta atgggcggta cccaaagtat acaatctcta
     2941 tcgcgcgtca tcggcccgct aatcgcagga cagctttatg ctgcagcacc atttcttccg
     3001 acggtattcg gatttttgct tatgattttt gcctattatc actacaataa aaaatagtag
     3061 cttcatttgc tacttcgtat aaattctctg tatcgaccga taaaacgaaa tactgtgtca
     3121 aattcttctt ttgacatatt ttcaaataca gtagcatctc gctgtaccca tttttgatgg
     3181 agttgctcgt gtctattcct tattttttgt ccgattggcg tcaattgaaa atagatttct
     3241 tttttattct cttcgctttg ataagtgttt atgatccctc tagctatcag tttttttgtt
     3301 agcttactga tggctcctct agtcatgtaa ctagcattcg ctaattttgt cacgttagga
     3361 tcagggattt tcccgatatg ttcaatcact tctagttcat ttagagtaaa tcctgcaaat
     3421 tctttttcca tttctggttt attacgatga ataatctcat tagctaaatc cagatattct
     3481 tctattagca gttgtctttt atccatttct acaccttctt tttctttcat tatattgttt
     3541 ttttgtttcc tattcaacaa agataaaaat attttgtagg aaaacccaat ctgaaaccaa
     3601 gaaaaaa
//
LOCUS       DASJSZ010000154         3577 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.161, whole genome shotgun sequence.
ACCESSION   DASJSZ010000154 DASJSZ010000000
VERSION     DASJSZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.161"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..207)
                     /locus_tag="U4B03_002767"
     CDS             complement(<1..207)
                     /locus_tag="U4B03_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069558.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSEN"
     gene            complement(505..1479)
                     /gene="sgrA"
                     /locus_tag="U4B03_002768"
     CDS             complement(505..1479)
                     /gene="sgrA"
                     /locus_tag="U4B03_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEN2069559.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1675..2514)
                     /locus_tag="U4B03_002769"
     CDS             complement(1675..2514)
                     /locus_tag="U4B03_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2069560.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2699..>3577
                     /locus_tag="U4B03_002770"
     CDS             2699..>3577
                     /locus_tag="U4B03_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN2069561.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNI"
BASE COUNT         1198 a          732 c          528 g         1119 t
ORIGIN      
        1 gttttctgaa agaaaagata atttatattc tggatacctt tcgcaaaacg attctgcttg
       61 ttgaatcacc gaattcatct tagccagggt gtagtttaga tctaaagcga tatccttcac
      121 cacatataat ttctcgtctc tttcataaca atagttcaaa aattgatata cttgcctttc
      181 attgatatca ataaattctt caaacaaagt acatactcct ttttatctat tatttagact
      241 tattccgaca gctacatgaa taacgtacaa atagattcgt tactgatttt tcagagatac
      301 taccatgcgc agtgtgcatt gtcaattatt cttgctctta ttttccattt gtgttcctgt
      361 tatttcctgt ttctcaaata tgttagtaaa caaaaataga tctggcaaag ctacaacttc
      421 gccaggcaat acagcactct cgaatgaaat attcactcaa aaatctatcg ttttattcaa
      481 ttttagatct gttgctttta ttctttattt cactcgatgg aagagagaac gaaaatagct
      541 tgctcctaca atcaaaatga gtaacacacc catccatgta attagcttgt tcaaggttct
      601 actaccagtt tctggtaaat cgctttttct attatctgtt tctttcgctt tttcttgatg
      661 atcaattcca cgagaaacaa aatcaggctg ttttagcaca ctttttaggt tgctccctgc
      721 atttctcttt tgttccttag ttggtatgat aggtaccaaa tcaagttcat tatttacttt
      781 tcctgtgctg ctttcgctgc tcgtgcttcc tgtgctactc tcactactca tgcttcctgt
      841 gctactttca ctactcgtac tctccgtact gctttcgctg ctcgtgcttc ctgtgctact
      901 ttcactactc gtactctccg tactgctttc gctgctcgtg ttttctgtgc taatttcact
      961 gctcgtactc tctgtactgc tttcaccgct cgtgttttct gtactgcttt cactacttgt
     1021 gctatccgta ctgcttggcg gtccaacaac tgtccaactg cttggtttca aattctccgt
     1081 gatcgtcatc tgtccatcat aaaaaacaat caaatcaact ttatctttac tcttgatctc
     1141 atcaatcgat agttctgcag aaatcattat tgtatactca ataccagcgg tccattgtac
     1201 gtcttttaaa taaacttccc caataacttt ttctgaactt ccattaccat cccaaccata
     1261 tgttagaaca gcatgtacat cactagcatt cacgcttttg tttggtttga ctggaatagt
     1321 aatgacttgt tgtgtcatgt cataaacaat ccgatcagtt ttcacagtaa atgaaagatt
     1381 ctcatttgcc cgttcatttg cagaagtact gattccaatc attgctgcaa ttatactcag
     1441 cccaagaata acaaataata cggtcgctgt tttcttcatt tctatcactc tccatataat
     1501 taccttctat ttgataaagg aattatataa aatgaaaatg atgagaacac gacagtcagt
     1561 tgtgaaaatc taacaaaaga tgtcgtgtat tcgcttgaat ttgtttactt tttcgtttaa
     1621 tcaatgacag acttaaaaaa gcaagaccga tatcagcctt gctttcttca tcttttattg
     1681 ataaacagca gtgtatttct tcatccgctt tgttttatat gcatagagta acaaaccaac
     1741 aatcaagact aacaagaata atgtagttga tatagatgct tcagccccaa agtttccccc
     1801 agtaattatt gtattttgcg attggatcag agtggagaaa aaaccttgtt cttgcgttcc
     1861 gctgactggt atacccaaaa tagctccttg tgtccaattc caagtaatat gagcaccaat
     1921 agccgtaaat aaacttcctg tactgtagat aagtgcgctg aaaaagcaac ctgctaaaaa
     1981 gatattgaaa actgccagaa atgtcatgtt aggattcaat ccgtgcaaaa gagcaaataa
     2041 tgcagcagat ccaatcattg ctattaacgg accaaatcta agcatctgat gctgaaaaaa
     2101 gccacggaat aaaagctctt ctgatgcccc cactgcaata aataaaataa aactcgttat
     2161 cagcggcaca atgtttgcgt ttcctgaatc taaccgatac gcccccgtca aatataatgg
     2221 actagtaata agcagaaaca ctccaattcc aacaacgaga cctcccgcta aataccccca
     2281 gtcttttcct cgaacagaaa caccataatc ttctattctt tttccttcga ccttactagc
     2341 aagaaataaa gctagtaaaa aaacagctaa atgtccgacc gtaccggcaa ttgctccaat
     2401 ccattcacta tttccaataa acatatccgc aagtgcatta ttgacaagaa ttcctaaaac
     2461 aaaaccaaca aagactatta aaacagatcc aatcaaccga ataatcctga tcatctttca
     2521 tccctccttt atattaagtt taacaaccct tttctttctt gtctcgtgaa aactatgtaa
     2581 atgttctcca tcatagagag aatagatttt tacgaactaa gaatatctac agcaacctta
     2641 cgaaaatgaa agactgtctc cctaattctt ctgttagact gtttttaata aggaaaggat
     2701 gggaaatttt ttgaaaaaac aactactaat tatgtgcctt tcagcatgtg caattttact
     2761 attctcaggt tgtagcaagt ccaatgaaac gattgcaacg tttaaaggtg gaacacttac
     2821 agttcaggat ttttatgata acccaaaagt taaatcaaat aatcaaacac tattaaaaaa
     2881 aatgattgtc ttcaaagcat ttgatgatgt atacggaaaa gagatttctt caaaggaagt
     2941 aacaaaagaa tataatgaac aaatcaaaaa actcggtcca aactacaagg aacaattaaa
     3001 agcagttggc caaacagaag agacatataa gaaactcttt aaacagatgc tggctttcca
     3061 atacggtttg aaagccaatg taaaattaac agataaagat ctagacactg cttggaaaga
     3121 attctatcca gaagttagta cacagatcat tctattttcg actaaagaag aagcagataa
     3181 agcaaaaaaa gaagcaaatg aaggagaaaa tttctccaag cttgttcaag cttatggaaa
     3241 aaataaaaca ttaaaggaaa ctgatggaaa aatgaatttt gattcaacca atccagagat
     3301 tcctactgaa gtaaaaaaag cagcatttaa actaaaaaac ggggaagttt ctgatatcat
     3361 tcctgtaaca gatccaacga cttatcagca aagctattat ctagtaaaaa tggtaaaaaa
     3421 acaggataaa ggttctaata aagacaaata caaatctgag ctagaaaaaa ttgctaccga
     3481 agcaaaaact cttgatacgg aattcatgga taaaacaatc cgtaaagtga tgaaaaaaga
     3541 caatgtaact attaaagatc cttatgtaaa aaatata
//
LOCUS       DASJSZ010000155         3561 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.162, whole genome shotgun sequence.
ACCESSION   DASJSZ010000155 DASJSZ010000000
VERSION     DASJSZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.162"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..149)
                     /locus_tag="U4B03_002771"
     CDS             complement(<1..149)
                     /locus_tag="U4B03_002771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEN2069562.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYS"
     gene            complement(139..1338)
                     /locus_tag="U4B03_002772"
     CDS             complement(139..1338)
                     /locus_tag="U4B03_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN2069563.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="U4B03_002773"
     CDS             complement(1468..2844)
                     /locus_tag="U4B03_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2069564.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..3500)
                     /locus_tag="U4B03_002774"
     CDS             complement(2844..3500)
                     /locus_tag="U4B03_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2069565.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
BASE COUNT         1089 a          666 c          490 g         1316 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt
     3241 tgaaatacta aagatatatt attttttcta tgattactat atcctttatt ttgtatatct
     3301 tcattcttaa acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga
     3361 agtgtagatt ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat
     3421 ttttgattta ctcctgacaa aactttttct ttagaatttg cataactata tgctacattc
     3481 tttatttcta atatatccat tattttctcc taacttattt ttgataagat ttctttaggc
     3541 ttcttgatta acattaatga a
//
LOCUS       DASJSZ010000156         3510 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.163, whole genome shotgun sequence.
ACCESSION   DASJSZ010000156 DASJSZ010000000
VERSION     DASJSZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.163"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            20..1009
                     /gene="galE"
                     /locus_tag="U4B03_002775"
     CDS             20..1009
                     /gene="galE"
                     /locus_tag="U4B03_002775"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEN2069566.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1021..2505
                     /gene="galT"
                     /locus_tag="U4B03_002776"
     CDS             1021..2505
                     /gene="galT"
                     /locus_tag="U4B03_002776"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEN2069567.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2529..3509
                     /locus_tag="U4B03_002777"
     CDS             2529..3509
                     /locus_tag="U4B03_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2069568.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1188 a          641 c          775 g          906 t
ORIGIN      
        1 aatcaaagga gtgtttttta tgacaatttt agtactaggc ggagccggtt atatcggttc
       61 gcacgctgta gaccagctag ttcaaaaggg gtatcaagta gctgtggtag ataatttgct
      121 cacaggtcac aaacaagcag tacatccaga tgctcatttt tatgaaggag acatccggga
      181 taaagaattt ctacgttccg tatttgaaaa ggaaccgatt gaaggagtaa tccattttgc
      241 tgcaagctct ttagtaggag aatctgtcga aaaaccattg atgtatttca acaacaacgt
      301 atacggtatg caaatcttac ttgaagtcat gcacgagttc aatgtgaata aaatcgtgtt
      361 ctcttctact gcagcaactt acggagaacc aaaagagtct ccaatcacag aagatacacc
      421 agctaatcct aaaaatccgt atggtgaaag taaattgatg atggaaaaaa tgatgaagtg
      481 gtgtgaccaa gcatatggca tgcgctatgt cgctttgcgc tacttcaatg tagccggagc
      541 aaaagcagat gcatctatcg gagaagatca tacaccagaa acgcatcttg tcccaatcat
      601 tttacaagta gctcttggtc aaagaaaagc attggctgtt tatggagacg attatgatac
      661 accagatgga acatgtatca gagattatgt tcaagtagaa gatttgattg ctgcacatat
      721 tttagctttg gaatacttga aagaaggaaa tgaaagcaac ttcttcaatt taggcagcag
      781 caaaggatac tcagtcaagg aaatgctaga agcagctaga gaagtgacag gaaaagaaat
      841 tccagcagaa atagcaccaa gaagagctgg agatcctagc cgattagtcg cttcaagtga
      901 aaaagcgaga gaggttctgg gttggaaacc tgaatacaca gatatcaaag caattatcaa
      961 aacagcatgg gattggcatg tcagccatcc aaatggttat gaagaatagg ggaatgagat
     1021 atgtcaatca gtcaaacgat ttgtgatttt gccacacttg cgatcgaagc tggcggatgg
     1081 atggaaatgg accgcttata tttacagaat cgtcttttag ggatggtagg agaagatgct
     1141 ctagaaagca ttgaagtaag accagttaag gaagaatctg tagacttatt ggatcagctt
     1201 gtcgctaagg caagagaaaa tggtgttctt tcagaattgc cagcagaaca agagattttt
     1261 gaagcgcaat taatggattt tctcacaccg ccaccttcag tcgtcaatgc attctttgca
     1321 cagcactatt cgaaaagccc agaggaagca actgagtatt tctttaattt aagtaagaaa
     1381 aatgactaca tcaaaacaag agccatcgca aaaaacatca aatttccggc agagtcggag
     1441 tatggtgaac tggaaatcac aatcaattta tctaagccag aaaaagatcc aaaacaaatc
     1501 gcagcagaac gcgaggcggt aaaagtagac tatcctacct gcatgctttg catggaaaac
     1561 gaaggatata gaggtcgctt gaactatcca gcccgcacca atcaccggat tattcgaatg
     1621 aatctagacg gtgaaagctg gggattccaa tattctcctt atgcttatta caatgaacat
     1681 tcgatcattt tatcagaaca acaccgcccg atgaaaatca caaaggaaac atttgctaga
     1741 ctgcttcgga ttactgaagt cttgcctcat tactttgttg gttcgaatgc tgacttgccg
     1801 attgttggag gatcgattct ttctcatgat cattatcagg caggacgtca tgaatttcct
     1861 atggcaaaag caccgataga aaaacttatc aaactagcag attatccgca agtcatcgca
     1921 ggagtcgtga aatggccgat gtctgtgatc cgtttgcaat caacagagaa aaaattgttg
     1981 attgaagcag ctaactcagt attggaaaag tggaaagcat attcggatga aacagtatct
     2041 atcagagctt tttcagcaga tggtacacca caccatacga ttacgccaat tgcacgaaga
     2101 agggacaatt actttgagtt ggaccttgtc cttcgagaca acaatgtttc agaagaacat
     2161 cctgatggga tttttcatcc acataaagat gtccaacata tcaaaaagga gaacattggg
     2221 ttgatcgaag taatggggct tgctgtttta ccacctcgtt tgaaaccaga actagtagaa
     2281 gtagagcatt tcttattagg agaagaaaac cagatggctg aatatcatcg tccgtgggca
     2341 gaacaattaa aaaaagaaca tcctgatttg acgaaagata cggtcaccga tgtcgtacaa
     2401 aaatctgtag gtcaggtttt tgcacgtgta ctggaagacg ctggagtatt caagcgagat
     2461 gaaaaaggac aggcagcgtt tgaacgattt actgcaattt tataaacgta aaaaaggagg
     2521 agagacaaat ggcaacaatt aaagacattg cgcagttggc aggagtttct cctgctactg
     2581 tttcaagagt tctcaactat gatcctgatc tttccgttgg cttagccaca aagcaaaaaa
     2641 tttttgaagc agcagaacgc ttaaattata cgaaacatcg taaaaatgca gcaaatacta
     2701 agcaaacgct tcgcctgatc cagtggtacg acgatacgga agagcttgaa gatctttatt
     2761 atttatctat tcgtctagga atcgaaaaaa aggcagaaga agcaaacgtt actttattga
     2821 aagaaacgtg ggattccatt agtgatcttc cgacagatgg aacgatcgca ctaggaaaat
     2881 tcgacgaagc tcagttatta gaaatgaaag gatcccaaaa tgccttgctt ttggtagatt
     2941 ctgacggcac ttctcaagga atcgactctc tagttgtcga ctttaagtca agtgttcaaa
     3001 aagtcattga acattttgtc tcccataaag cgcaacatat cgctatgctt gctggaacag
     3061 aatatacgaa gaaaaaccat cagcggctaa aagatcccag aattatggca tttcaacaag
     3121 ttttggcaga aaaaggctac gacgaaggtc caattatcga agcagatttt tctgtcgagt
     3181 caggatatca agcagtgaag cagtatttaa aaacgaatcc gcatgttcct gatgcgcttt
     3241 ttgcagctaa tgatgcatta gcgattggcg cactgaaagc gattcaagaa aaaggtttag
     3301 tggtaccaga agatatttct gtgatgggtt tcaatgatgt cagtgtagcg aaatatgtaa
     3361 cgccctctct ttctactgta aaagtttata ccgaatggat gggagagcta gcagtggaga
     3421 cgattcttca attaagtaat tccaaagcac cggtttcaag aaagattatt gtggaaacag
     3481 agttgattat tcgagaatca acgaaataaa
//
LOCUS       DASJSZ010000157         3388 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.164, whole genome shotgun sequence.
ACCESSION   DASJSZ010000157 DASJSZ010000000
VERSION     DASJSZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.164"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(63..2555)
                     /locus_tag="U4B03_002778"
     CDS             complement(63..2555)
                     /locus_tag="U4B03_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN2069569.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="U4B03_002779"
     CDS             complement(2562..3029)
                     /locus_tag="U4B03_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEN2069570.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3165..3238)
                     /locus_tag="U4B03_002780"
     tRNA            complement(3165..3238)
                     /locus_tag="U4B03_002780"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3202..3204),aa:Arg,seq:acg)
     gene            complement(3296..3369)
                     /locus_tag="U4B03_002781"
     tRNA            complement(3296..3369)
                     /locus_tag="U4B03_002781"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3333..3335),aa:Pro,seq:tgg)
BASE COUNT          924 a          750 c          557 g         1157 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tcgacgcgcc caagaggagt
     3181 cgaaccccta accttttgat ccgtagtcaa acactctatc cagttgagct atgggcgcat
     3241 attcaattta aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa aaaaatcggg
     3301 aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc
     3361 tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DASJSZ010000158         3280 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.165, whole genome shotgun sequence.
ACCESSION   DASJSZ010000158 DASJSZ010000000
VERSION     DASJSZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.165"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(238..963)
                     /locus_tag="U4B03_002782"
     CDS             complement(238..963)
                     /locus_tag="U4B03_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069571.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1117..1443)
                     /locus_tag="U4B03_002783"
     CDS             complement(1117..1443)
                     /locus_tag="U4B03_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069572.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1480..1845)
                     /locus_tag="U4B03_002784"
     CDS             complement(1480..1845)
                     /locus_tag="U4B03_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEN2069573.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1882..2097)
                     /locus_tag="U4B03_002785"
     CDS             complement(1882..2097)
                     /locus_tag="U4B03_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2069574.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2226..2561
                     /locus_tag="U4B03_002786"
     CDS             2226..2561
                     /locus_tag="U4B03_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2069575.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2637..>3280)
                     /locus_tag="U4B03_002787"
     CDS             complement(2637..>3280)
                     /locus_tag="U4B03_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069576.1"
                     /translation="NRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEK
                     FHFTYQYFSYEWVKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTY
                     LMTEKELINTVCERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASS
                     SFLVYLAKGAGIDEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
BASE COUNT         1024 a          651 c          442 g         1163 t
ORIGIN      
        1 tatacaaatt tcttaaaaat tttttcacta aaatcactac ctgaaacagt ctaaaacact
       61 tatatatcaa cgtttaatga aactatctcc gtttcatttc agatacaaat aaatatagtc
      121 gggctccaaa tccgaaaaaa aataccccca gtagcttatc ctaagctact ggagatgtat
      181 aagtacggtc atataacaac ggggtagggt aaataaatga aaaagatgga agaatcatta
      241 ttcaataact tttttaaata cttcgttgta atcagttacc tgtccgttct taaataccac
      301 agaataatct tttttcccat tttcaagtaa ccgaagtgga atatctttac caactttctc
      361 tgtcactgca ttactgatac ctgttagatt agctaataaa tctgataaag attgagcatc
      421 tgtagaaatc tctgtatcag tatttatcgt gattccttta tcttcattta caagtttcca
      481 agtaggtggt atagctgtgt atgtttctac aaatgaatta agtttttcat taagtgactt
      541 ctcttttata tttccatcta aaaactctgt atatatggct tgagctatac taaaacaaag
      601 ctggccttgt tctaataatt ttacatcatt agttagtaaa gctgtgttca cgttatcaat
      661 cgcttcaaga agagtatctt tggtgtccct aacagcttta ggtgtgcctt ctggaatttc
      721 cctacttgat acctcgtcca ttttatcttc cataaacccc ttaaaatcat atctttctcc
      781 caaatccata gaagtttttg agaaagcttt catttctgag tctatttctt caataatatt
      841 taacatgtct gatacatatt gtttaggctg attcgttttt aatctatatg tggtaaatat
      901 ctcaattcct ccaattatca ataaaattgc caaacaaatc cataataatt ttttcttttt
      961 catttttcct cctatacact gctgttataa cagtgactaa tgtaaagatt ttcccagcaa
     1021 tcatcagact attatttaca gggaatcaaa aaaactcctt agtaagctta cacctttagg
     1081 agttcctgtt tgaacggtat gcataataca tttaaatcaa tttaacatta acacctcagt
     1141 atatggtatc tctacatata cacggtcttt ttttggatag tactcgacat ttatattgat
     1201 agcacttcca ttgtctaacg tagctttagc ccggtacgtg ttatcaccat tatcatgagt
     1261 gatcgtcaca tcatcacatg tccgatttaa gttatactgt tcttgtaaaa tctgagttac
     1321 caatggtaca gatgacttct ctagtccaga tgaacccgtc attcttgaaa aaattatacc
     1381 tataatagcc aatacaacga tacctataat gacaaacttc ttattcatat ttactttatt
     1441 caacttttta tctcctatct ccaaaatgat tttctctcat cagtaactca ttgacatccc
     1501 caatagtaca ccaaagatgg ctccagctac ccctgctatc atcaaaatcg tacccatttg
     1561 ttcatctttc tctcgatata agtaacccat aataacagca acagcaccaa ataccacagg
     1621 aataaatgct aatgatacta ccatagaaat ccaaccaata atcaaataca gcttccctgt
     1681 ctcttgtcca tgtacgattt gttccttcat ttcattaatt gtctgctcag tacttttctt
     1741 tactgtgtct gttccggttg tctcatctgt ttcttgtaat gctccgcaaa actggcaata
     1801 cttactttca tttgttattt ctttaccaca ttttctacaa tacatgtctt ctcctcctaa
     1861 aatcattcaa ttgcttatta gctaactgtt tactttatta aatacatcag aaaacagctc
     1921 atttattggt acacttaata tctcagaaag cattttcatt tgatagggtt taggaatcgt
     1981 tctccctgtt tgccacgaac ttacacattg ctgtgtaaca ttcatttttt tggctaactc
     2041 agtttgggaa agtccttttt cttttctgta tcggctgaga ttacttactt ttttcatgat
     2101 tctcccctcc tgttttatta tatacaacaa tatgatgtat ttcaactcat aaaatatata
     2161 aatacttcaa atgtcattac aaatacaaca acttgttgta ctctactgag aaggggggtg
     2221 tattaatgaa acagaagcaa tatgcaaagg tcattagaga aaagcgaaaa gcactcgggt
     2281 ggacacaaaa agaattagct aaaaaaatct tctctacaca acaggcagta gcaagatggg
     2341 aaaattctgt aacagaacct aacctcgata gcttaacagc tctttctaga gccctaggaa
     2401 cccctgtaag ccattttcta gacaatgttg acatagatta tgaggaagag tttttggctc
     2461 tctatcgctc tcttagtact gaagatgcaa ttcgaacaat tgactatatg aagctattaa
     2521 aaaggcaaga aaatgaacgc aatcagctat tgaaagattg aaaaataaat aacccaccga
     2581 actttaacgg tgggtgtatt gagatgaaat aagattaaga gctatggata acctgtctag
     2641 tttaatataa ttgggtctct tctattcctt actctccgtc catcatctct tgttacactt
     2701 gtaaattgat aaataggaac taattcaact cctgataata tcaattcttt tattacttca
     2761 tctatccctg cccctttcgc tagatatact aaaaaactac tgcttgcttt cacattgtat
     2821 agtttcttaa tatccttaga agctcgttca agagtgaatc cttgtttaac aaagtagcct
     2881 ctgttttcta ttaaagcatt tttcacgctg ttatccctga catttcgtag gcgttcacaa
     2941 acagtgttaa taagttcttt ttctgtcatt agatatgtcc cttgccacga taagccctgt
     3001 aaaacttcat gtgcttcatc taaaatcaat tgtttttcct tgctaagttc tgatcgggaa
     3061 ttttgaggaa atactctttt agcctctttt tcgttaaaac atatcttaac ccattcataa
     3121 gaaaaatatt gatatgtaaa atgaaatttt tccagaagtg gaaggatatc ttcattcaat
     3181 ttttgaatca cagaagttgc attaaaaacg tctaatcgat agacattgct tctcttatgt
     3241 ttaaagtttc ctaacgcttt ctcttccttt aacctattta
//
LOCUS       DASJSZ010000159         3035 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.166, whole genome shotgun sequence.
ACCESSION   DASJSZ010000159 DASJSZ010000000
VERSION     DASJSZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.166"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            159..425
                     /locus_tag="U4B03_002788"
                     /pseudo
     CDS             159..425
                     /locus_tag="U4B03_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1808..2974)
                     /locus_tag="U4B03_002789"
     CDS             complement(1808..2974)
                     /locus_tag="U4B03_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEN2069577.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT         1019 a          581 c          418 g         1017 t
ORIGIN      
        1 gacctaaata tcaatatcca tttcgaggaa aattgtttaa ctaaagaaaa atacaaagga
       61 caaatttgct tgatttacaa agggactctt cagtacacac cagaagaata tccacatctt
      121 ccaatatatt aaaagaacct tcttgaatca ttgagtgaaa gaaaagagat tcgtcactat
      181 ttgaggactg tttaactaga gacattagcg atctgcctga aacctataca accactttac
      241 gaacgtttaa aaattttctg ccacagattc aaaatgcgct taactgcagc tattcaaaca
      301 gaccattgga atgtttaaac aaccacatca aagtattaaa acgcaatacg tatggctttc
      361 gcagctttta caatttcaaa ctacggatca tgattcgtca tggcaaaaca tttttaacca
      421 aataaacaga agaatgtggg ataaatctaa aaatcagttt tgctacagac tctcgagtaa
      481 acggcgggag cagaagcaac tccttcggaa ataagccgaa attcacaaaa atttgaaaag
      541 caattttcgt gaatttcttc ttatttctcg gagttaaaca cttctgtccc agcctcttct
      601 gtccaaatta ttctaagatt tctctttacc aacatcattt gacaaagagc ttcttttttg
      661 aatactatat tcgttctgtt tttcatcttc aaatacgaac tcatccgcag aaatatttgt
      721 cacaactcca tgaatcttct tttcattacc acctatatac cacaggattc ctacacagaa
      781 acaacaaaaa aatagcataa aataggtttt ctttatttta tatccctcct tttagattaa
      841 aagctcacag aaaacgcttt ctaaaaagac aatagaataa tcataatttt tcattttgta
      901 ttatttatac aaataatacg catgttactg aaaaatgata tggttcccta ctttttgaga
      961 taagttaaat aaaatctgtc atcgataaaa cagctagtac agtaatgata aatagtaaga
     1021 acttaaaata ttttggattt ttatttttga aataatcgaa gaaaagtaaa agtgataacg
     1081 aaatagcaaa caagattata tctataagat tattcattga gaaatcgccc catatggaac
     1141 tctattttga cctgacacac cgagacataa agcaatcata tcctctgcat tgttttatac
     1201 tattagaact atagctacac ctattggatg ctactggctc taagattaat gctaatacag
     1261 taacatcaat acctaaacta gcagaaactc tatttgcatc ttcaatttca gatgcctgga
     1321 ctgtcgtgac tagcgccgta gtagaaaaat ttactggtac taacaaagat gcagacacga
     1381 cttttatttc acttatcctt tatcccataa cctaaacttc aatcattact tgtgagctga
     1441 tcaatgttct tccaagtctg aaagaacatt gatcagcttg gcaaacaaat aatgttttca
     1501 aatacttgat cgatgaaaaa aagctattgg taataacagt aattgcattt tccattttcg
     1561 tgtcagatac ttcgcttaca gctggtaaac gatcaatccc attgtcaaat tttggatttt
     1621 tttctgatga tgtcttgctt tagttcataa aataatccag caataaattc ctcgcaagga
     1681 tagccaactt cattttccta atcggaattt ttaaacttat cggtatagat acagatgcaa
     1741 tgagaacaaa catttatttc gagtcaccat ctaaaatatt ttcctccatg tacccgttca
     1801 ttattattta gttatcacca aagatatctc tggtataaac tttttcttca acatccttta
     1861 aaatatcttc aaaccgatta gatacgatca catcagaaat atttttaaat tcattaatat
     1921 ctttcaatac ttgtgaatga taaaattcat cttcatccaa tgtaggctcg taaacaacca
     1981 cttcaattcc tttggcttta atccgtttca taattccttg gatcgacgaa tatcgaaaat
     2041 tatctgaacc ggccttcatc gttaaacgat agattccaac aacatttggt tttttcgcca
     2101 aaatctgtcc agcaataaaa tctttccttg ttgtatttgc atcaacaatt gcactgatga
     2161 tattgcttgg tactttttca tagttggctc ttaattgttt tgtgtctttg ggtaaacagt
     2221 atccaccata gccaaaagaa ggattattat aatgcgaacc gatccgtgga tccaaaccga
     2281 caccttcaat aatttgctta ctatcaagcc cacgaaattc tgcatacgta tctaattcat
     2341 tgaaatacgc cactcgcaat gccaaatatg tattcgagaa taatttaata gcttctgctt
     2401 cagttgagtg agtcaaaaga actgatgcat ctttgttaaa tgcgtttctt tgaaacatag
     2461 ttgcaatctc gcgaccacga gcagaacatt ctccaactat aattcttgat ggatataagt
     2521 tgtcgtacaa agcttttcct tcacgcaaaa attcaggtga aaaaatgatg ttttctgtac
     2581 caaatttcgc tttaatttca tttgcatagc caactggaac tgtcgactta ataatgaatg
     2641 ttgaatctgg acgaattgct aaagccttct caattacatc ttctactgtt gatgtattaa
     2701 aatagttttt cttttcgtca taatctgttg gagttgcaat aatcaagtaa tcaccgaagt
     2761 ttactgcatc agtaaaattt gacgtaaatt cgacatttaa gtcatcccgt tttaaaaatt
     2821 catgaacttc tttatcttct attggtgaga tgccattttg taacatcgtg attttttctt
     2881 ccacgatatc ataagctttg acctgctctt tttgtgctaa taatagtgaa tttgctagac
     2941 caacatagcc taatccgaaa actgaaattt tcatattctt ccatacgctc caatcaaatt
     3001 tcattttata gtattttagc tgttatcctt ttgaa
//
LOCUS       DASJSZ010000160         2936 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.167, whole genome shotgun sequence.
ACCESSION   DASJSZ010000160 DASJSZ010000000
VERSION     DASJSZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.167"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(111..386)
                     /locus_tag="U4B03_002790"
     CDS             complement(111..386)
                     /locus_tag="U4B03_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069578.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            618..1352
                     /locus_tag="U4B03_002791"
     CDS             618..1352
                     /locus_tag="U4B03_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2069579.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            1391..2533
                     /locus_tag="U4B03_002792"
     CDS             1391..2533
                     /locus_tag="U4B03_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2069580.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         1104 a          471 c          482 g          879 t
ORIGIN      
        1 ttgatgtttc catttgtgtt tcctccttat aaaattctgt taaagactaa aacatacaga
       61 taataaaaac aaactcttta ttactatatg ttttagtctt atttgtttac tcaaccttta
      121 taccgttcca tgaaactatc aatatcagat tttttaaatc taatgaccgt atcaattttt
      181 ataacaggta acccgtattg agaaacatat tttctaatcg ttcctactga tacacctaga
      241 tattcacttg cctgctgata gtccatatac tctagagtga ctccagaaat aatgtcttct
      301 agcataggct ttagcactgt aattatctct ttcacttgtt gttcaacttg tatagaaaca
      361 atgctatcaa tgatttgtag agacatattt ccccctcctt tcgtgattgc ttgaacaaat
      421 tttctaagaa ttatcttcgt attttttgct caaaataaaa gtattttgtt attaaccgat
      481 taatcaaaca aaaaatgatt accagttcat cgacatacac ataatacctc gataaaaaag
      541 gggtgtcaat agttttgtaa aatttattta caacacccct tttttgaggt atactaattt
      601 taggaggtgt ggtcaatatg ataaaacgtc caccaataaa ttatttagaa cgaaaaaaga
      661 tactcggaac aaaaataaaa gctataagaa agagtaaaaa attaacacaa ccagcatttg
      721 gtcttatgat taacaacggt caattaattg acaaaaagac tatctacgaa tgggagaagg
      781 gaacctattt accaatacca gagcgattat cccgaattgc tgatttggga aatatgagta
      841 tagaggagtt agtttgtggc aatgtagaag aatatatttt gggaattatt ctttatagag
      901 atagcattgt actagatggt ataacatttc cagataaaaa cctgttccag catcttcgtc
      961 aacagttccc accagtacac tctaatttag atacatggct ggatagatat tccaaattag
     1021 aaccagagat gcaagaattt attgctaaca agacttgtaa taaagttaaa aatgaaaaaa
     1081 ttagtctttt caatatctta aaaatagaag aattattcat caatgctatt gtagaagaat
     1141 ttgacaataa cattcttttc ctcactagca gtatagaaga actactagaa agaatggtag
     1201 atgaatggct acctattcaa ttgaaagaca tgtcttatcc cgaagaagct gtgagagaaa
     1261 ttactgataa tataaacaag ctagaacaaa cgatttcatc aattgggaaa aaatacacta
     1321 agaaaaaaat gaagggaggt gataccatct agtggtaagt actccttgta aactaagagg
     1381 agatgatttc atggcaaaaa tatcaaagta caaaaagaaa aacggtcaag tggcgtggat
     1441 gtataaagga cacattgcca ccgatccacg aactggtgaa aaaataaata ctacgagaag
     1501 aggttttagt agcaaacaag aagctcaaaa agattatgac gagtacagac atcaaatatt
     1561 atacggtgtt aaaaagaaag cacctgatat gacatttgaa gacctgtata atgaatggat
     1621 aactcatcag cgtaccagtg ttaaagcttc aacaatcgca atctcagtaa gatacgcaaa
     1681 taatcaaata cttccagcat ttggtaagct taaactatcg aatatctctg tcccatattg
     1741 tcaaaaagtg gtagatgaat ggcacagtaa atatgaaagt tacgattata tgagaaaaca
     1801 gactgcacaa attttacgct acggggttgc aatgcaatat attgacaata atcctatgga
     1861 aaaaacactt cttccacgaa aaaaagagta tgagaaaaat cgtaaatttt actcaaaaga
     1921 ggagcttaat aatttactgg atgcttttaa agattttggt aacatgaaac aatacgcctt
     1981 cttcagatta ttggcataca caggtatgag aaagagtgaa gtactcgctt tacagtggaa
     2041 agacatagac acgtttaata aagaactaca tgtcaataaa actcttgctg tagatgaatt
     2101 tggtaaagtt ataattcaat ctccgaaaac aagagcttct agacgagtta tctctttaga
     2161 tactgagact ctttctatcc tcaacaactg gaaattacag caaaaagaag agtatttaaa
     2221 acttggatac aatactagct ctaaagagca acatgttttt acaacagtta aaaacactct
     2281 atacattcca aacactgtta atgattggtt aagatatatt ttgaaaaaat ataacttgcc
     2341 tcgtattaca cctcatgggt ttagacatac acatgctagt ttattattag aagcaggaga
     2401 atctgtcaag gtagttcaac aacgattagg gcatgaaaat agtaaagtga cattagacat
     2461 ctatgcacat attacaaata atgcacctaa gaaaacaggt caagactttg ctgatatgat
     2521 ggctcatcag taaaagagct ggtcaaaatc tggtcatttc tagtatacag gcaaaacaaa
     2581 aaagctagaa cccttatata tcaagggttc tagctcctat aacaggttta cccaacactg
     2641 ccttccaatg actcttttgg gaatcagagg aaagaatcag gctttataga gatatttcat
     2701 ctaacaaatt actttagact gacagtatat atcagcaggt actgtcatcg ggaacgtagc
     2761 caatatcgta tccaaaacag caggtagaga agctatttga ggattcaagt tattctattt
     2821 caaccatgaa gttaagttca ttaaaaataa agatagttgg tatttcaata atttttgtat
     2881 agagttaatc actttattga tgctaattca tcttgattgg tattataatc gtattc
//
LOCUS       DASJSZ010000161         2904 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.168, whole genome shotgun sequence.
ACCESSION   DASJSZ010000161 DASJSZ010000000
VERSION     DASJSZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.168"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1087
                     /locus_tag="U4B03_002793"
     CDS             <1..1087
                     /locus_tag="U4B03_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2069581.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="U4B03_002794"
     CDS             complement(1305..2447)
                     /locus_tag="U4B03_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2069582.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="U4B03_002795"
     CDS             complement(2452..2637)
                     /locus_tag="U4B03_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN2069583.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          965 a          531 c          453 g          955 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caat
//
LOCUS       DASJSZ010000162         2810 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.169, whole genome shotgun sequence.
ACCESSION   DASJSZ010000162 DASJSZ010000000
VERSION     DASJSZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.169"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1682)
                     /locus_tag="U4B03_002796"
     CDS             complement(<1..1682)
                     /locus_tag="U4B03_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2069584.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYR"
     gene            complement(1642..>2810)
                     /locus_tag="U4B03_002797"
     CDS             complement(1642..>2810)
                     /locus_tag="U4B03_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2069585.1"
                     /translation="TVEYIRGIQVIKIFGAKVTSFKALNKAINDYAKFAYKYSLSGKT
                     PFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVDLLMLFFLSGIIFVSFMRIMYVS
                     MHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNCDIAFENVSFSYKEECVLENFNL
                     VLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQIKIGGKPLNHYSKDTIINTIAFV
                     FQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGCESILEKFPEREHTLIGSKGVYL
                     SGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEYELQKAFQHLMKEKTVIMIAHRL
                     SSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQLVTNYENANDWRLDNEKFL"
BASE COUNT          918 a          537 c          401 g          954 t
ORIGIN      
        1 cgatacaact cattatgagt accttgatca actagttttc cttgatccat cactacaatt
       61 ttatttacct tttcaataga cttcaagcga tgcgatataa ttagtactgt cttatcttgt
      121 attaaacgat ttaaactttc ctgaatacgt ttttcatttt ccgcatctaa actggcagta
      181 atttcatcta aaattaaaat tggcgcattt ttcaaaaaag cccgtgctat ggataggcgt
      241 tgtcgctctc ctccagaaag tgaacttccg ttttctccga ccaatgtatg ataaccgtca
      301 ggtagttttt caataaagtc ttcgcagtta gctaaacgag ctgctttttt cacttcctca
      361 tctgacgctt gactatttcc aatacgtata ttttccaaaa tacttgtatt aaacaaagtt
      421 acctcttgaa aaacaatagc gattttgcta aacaaagaag ctggagatac acgtttaata
      481 tcatacccat caattaaaat acagccttca tcataatcat acagtctaga cactaatttc
      541 aagatactag ttttcccgga accactagca cctactaacg cagtcacttc tccttgttta
      601 gccgtaaaag agatatgatc taaaattggg gtattgttat catatgagaa agagacatct
      661 cgcaactcaa tgtcaaaaga tttcagagga ctatctgatc cctcttggat actggtttcc
      721 ttcatagctc taattcgctg tattttagga gctaaataaa atatttcaag cactgcttct
      781 ttcatagaat cgaaggaatc ctttattttt attgcggcta gtaagtaacc aacgacgtag
      841 agtatagtca cttcacccgt taacagtaag tgaacaccta ctagaatgac tactgctaaa
      901 ctaacatatg agaaaataga ggataaggcc atgactgaaa aagtacttaa ttctaccttt
      961 aaatgaatcc tttcactctc ttccattttt ttgaaaagtc tgtcttgaac ttttttagat
     1021 agattaaagc tattaatctc ttgctgcaat tctatagttt cttgaaattc ttccgagttc
     1081 tctctcaaag tatcgtaata tttagtattg gctttcgttt gggatttctt tgataacaag
     1141 attaagacaa aactaaataa cgttggcaaa ataacagcta atcccatttt gacattacca
     1201 accaaaagca tcactgaaat aaaagggaaa aacagagcca taccgccggc tttaggtatt
     1261 gcatgactca tcgcatgctc aataccttca acgtcagaca tgatagtttg agataaatct
     1321 gataaattat gttttgaaaa ataggataat ggtaaatttg acaaatcgtc tgcaatttgc
     1381 actcttaaat gcgcactttc tttataagta ctattatata agttttcata ctccctatct
     1441 aacaaccaat acatgaaaag caaagtaaca aacgatacgg caaaataaag ccaacggggc
     1501 tttgtatttt ctaaaactaa ttcgtctatc agcatcatta taatgaatgc tggtaccata
     1561 ttaatgcagt atacaaaaaa actagaaata gaagctttag ttaaagcttc tgcgccctga
     1621 tcacttaaag caaatttgtt tttataaaaa cttttcattg tcaagcctcc aatcatttgc
     1681 attctcataa ttagtgacca attgtttata cacttgatgt ttagccatta attcattatg
     1741 atttccacgt tctactattt tcccttctga caagacaaga atttcatcta cagatttaat
     1801 gctggataat cgatgagcta tcataataac tgttttttct ttcatcagat gttgaaaagc
     1861 cttttgtagt tcatactcat tatctggatc aatagatgca ctggcttcat ccataataac
     1921 aatctttgca tcttttaaaa ttgctctggc aatagctata cgttgttttt ctcctcctga
     1981 cagataaact cctttactac caattaaggt atgctctctt tcgggaaatt tttctaaaat
     2041 cgattcgcaa ccggctaatc ttagcgcgtc aaatacttta cttgctggcg cttgaggatt
     2101 tgctatagaa acgttatcgt aaatactctg attgaatagt ttcgtatctt gaaagacaaa
     2161 agcaattgtg ttaataattg tgtcttttga atagtgattt aaaggcttac caccaatttt
     2221 aatctgtccc ttatccacat tgtagaaacc tgaaattagt ttagctatcg tactctttcc
     2281 gcttcctgat tttcctacta atgcataaat tcttttttgt tctaaaacta agttgaaatt
     2341 ttctaaaaca cactcttctt tgtatgaaaa gctgacattt tcaaatgcaa tgtcacaatt
     2401 tttaatctgt gtctcgttcc catattttag ttggtcttgc ttcatttcta aataaaggga
     2461 gtctagtgtt tcgactgcat atcttgcttc aaacaagtgc atacttacat acattattct
     2521 catgaaagaa acaaaaataa ttccacttaa aaaaaacaac atcaacaagt caacagctaa
     2581 tatcttggga gtggtaagag aagttataaa aaaggttata ggaactatca aaatgcaaac
     2641 tatgccaaaa aatagccatt gaaaaagaac aaaaggtgtt ttcccactca aagaatactt
     2701 atatgcaaac ttagcataat cgttaattgc tttattcaag gctttaaatg atgttacttt
     2761 agcaccaaat atctttataa cttgaattcc gcggatatat tctaccgttt
//
LOCUS       DASJSZ010000163         2715 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.170, whole genome shotgun sequence.
ACCESSION   DASJSZ010000163 DASJSZ010000000
VERSION     DASJSZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.170"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            56..502
                     /locus_tag="U4B03_002798"
     CDS             56..502
                     /locus_tag="U4B03_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2069586.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            775..2127
                     /locus_tag="U4B03_002799"
     CDS             775..2127
                     /locus_tag="U4B03_002799"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEN2069587.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2298..2585
                     /locus_tag="U4B03_002800"
     CDS             2298..2585
                     /locus_tag="U4B03_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069588.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          956 a          447 c          540 g          772 t
ORIGIN      
        1 ttttaaaatg taaaaatagt atgataagac gagaataaca acgtggggtg tttacatgga
       61 aaataagtta gtcgaagagg ttatcgaaaa gttaaaaaaa gagaacattc gaatcacacc
      121 tcaacggtac gcggttttag aatatttgat tgaacatcat tcccatccaa cggctgatga
      181 gatttataaa gcacttgaac atcggtttcc aaatatgagt gttgcgactg tttacaataa
      241 ccttcgctta tttacttcga ttggatttgt tcaagaaatg aagtacggcg atgcttctag
      301 ccgatttgat ttcagttcaa agcgtcatta tcatgtaatt tgtgaaaact gcggtaagat
      361 tgttgatttt cattacccag ggcttgaaga tatagaaatg gctgcaagca gactgactga
      421 ttttgagatt aatgaacatc ggttggaatt gtatggactc tgtccggatt gtaaagagtc
      481 agcaaaaaag gcgcaaatgt aaaaacagtt tctacaagtg ttatacctaa aaaaagctag
      541 ctgtgtaaaa aaaacgctag cttttttatt tttttgtctt ttattcacaa tcttagatat
      601 aaaagtgaat attattaaaa taagaatctt gtagatattg ggttcttatg cttgaaaagg
      661 aatgaaacta caaacaaacc tttgtgaatt cacaagcatt aaactgttgt atccgtttta
      721 tttctgttat agtagttttg tcgaaggaca aaatgttaat cggagggacc ataaatgaaa
      781 gtagtagtaa ttggctgtac tcatgcaggt actgcagctg taaaaagtat tttaaaaaat
      841 catccagaag cagaagtgac agtatatgaa agaaatgaca atatttcttt cttatcttgc
      901 ggtatcgcac tttatgtcgg cggagtagta aaggacccag caggcttgtt ctactcaaat
      961 cctgaagaat tatcttcatt aggagcaaca gttaaaatgg aacacgatgt aacagacgtc
     1021 gatacagaaa ataaaacagt aacggcaaaa gatctgaaaa caggagaaac agaaacggtt
     1081 tcttatgata aattagtgat gacaactggt tcatggccta ttatcccacc gattagtgga
     1141 atcgatgcga aaaatgtact cctatgcaaa aactacaatc aagcaaatga aattattgca
     1201 caagcgaaag aagcaaaacg tgtagtaatt gttggtggtg gatatatcgg tattgaatta
     1261 gtagaagctt ttgtcgaatc tggaaaacaa gtgacattga ttgatggatt agatcgcatc
     1321 ttgaataaat atctagataa accattcaca gatatcttag aaaaagaatt gacagaccgc
     1381 ggtgttactc ttgcgctagg cgaaaatgtt actgaattta tcacagatga agaaggaaaa
     1441 gtgaaacaag tagctacacc aacagataca tttgatacag atatggtcat tttatgtgtg
     1501 ggcttccgtc caaatacaaa actgatcgaa ggaaaagtag atacactgcc aaacggggca
     1561 atcaaagtga atgaatacat gcagacaagt aatccagaca tttttgcagc tggagactct
     1621 gcagtagtca actacaatcc aagtggcacg caaaactata ttcctttggc aacaaatgcc
     1681 gttcgtcaag gtttgcttgt gggaaataac ttgacagatc ataaaatggc gtatcgtggt
     1741 actcaaggaa cttcaggact ttacctattt ggctggacga tcggttcgac aggtgtgacc
     1801 aaagaaagcg caactttgaa cgatctagaa gtccaagcga ctgtatttga agataattat
     1861 cgtccagagt tcatgccaac tactgaaaaa gtaatgatgg agctagtcta tgaaaaagga
     1921 acgaatcgta tcgtaggtgc tcagttcatg tccaaatacg acattacaca atcagcaaat
     1981 accatgtctt tagctgttca aaataaaatg actgtagaag atctcgcact ttcagatttc
     2041 ttcttccaac cacattttga ccgcccatgg aattacttga acctacttgc acaagcagcg
     2101 ctaggcgaaa tcgaaactgc acaataaaga aaaagggttg tgacacaatt ttgtcacgac
     2161 cctctgtctt tttttgtgaa aagtacaatt agttgtcttt cttcgaattt tttgtcacaa
     2221 taagcagcaa agataaagct gttacataaa agaacaagca gctttatttg attttgaaaa
     2281 gaaaggtgag aaagaagatg aataaagaat attttgtcca acaagatgca tttttagaag
     2341 attataaaag tgcttcgaag aaattaacga tcaatattga tgagttggca gatcaagtac
     2401 tctctcagtt gaatgaaacg caagaggaat attacacgat gccagcaaat gacgccaaag
     2461 ataatgtttc tcatcgtttt cctttcagta gacgcatcta ctcagatgaa aatgacggca
     2521 cattgaatac agagtacaca tatgaaggtc agccatatgt gctagaaact gatgatgatc
     2581 tttaaagaca gtttttgaaa aggccgctca tccttaagtg atgacaaaaa atcatcacaa
     2641 ttaccgatga ctgtctcttt actatctttt aatcagaaaa aggggttatg acaagctttg
     2701 tcaacccctt ttttt
//
LOCUS       DASJSZ010000164         2675 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.171, whole genome shotgun sequence.
ACCESSION   DASJSZ010000164 DASJSZ010000000
VERSION     DASJSZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.171"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            690..785
                     /locus_tag="U4B03_002801"
     CDS             690..785
                     /locus_tag="U4B03_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN2069589.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1332)
                     /locus_tag="U4B03_002802"
     CDS             complement(1087..1332)
                     /locus_tag="U4B03_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069590.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="U4B03_002803"
     CDS             1677..1961
                     /locus_tag="U4B03_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069591.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="U4B03_002804"
     CDS             1964..2380
                     /locus_tag="U4B03_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069592.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          882 a          497 c          430 g          866 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggatt
//
LOCUS       DASJSZ010000165         2581 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.172, whole genome shotgun sequence.
ACCESSION   DASJSZ010000165 DASJSZ010000000
VERSION     DASJSZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.172"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1412
                     /locus_tag="U4B03_002805"
     CDS             <1..1412
                     /locus_tag="U4B03_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2069593.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2581
                     /locus_tag="U4B03_002806"
     CDS             1546..>2581
                     /locus_tag="U4B03_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2069594.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLK"
BASE COUNT         1014 a          360 c          518 g          689 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 a
//
LOCUS       DASJSZ010000166         2513 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.173, whole genome shotgun sequence.
ACCESSION   DASJSZ010000166 DASJSZ010000000
VERSION     DASJSZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.173"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            187..>2513
                     /gene="esp"
                     /locus_tag="U4B03_002807"
     CDS             187..>2513
                     /gene="esp"
                     /locus_tag="U4B03_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEN2069595.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          916 a          388 c          503 g          706 t
ORIGIN      
        1 ttaaaaagca caaatatccg aaaaaaacat gaagaaatgt actttttttg ttgtaatgtt
       61 catgaaattc tctaagtaaa ttagttaaca taaggttagt aagattccta ttctgtgaat
      121 aatacgtatc agatttatct tttattctta ctaggaaagt aattattaag ggagttgatt
      181 tgaataatgg ttagcaagaa taataagaga gtatttctag aaaaaacaaa gaaacgagta
      241 ttgaagtaca gtatcaaaaa attaagtgtt ggggttgcat ctgttttgat tggtgttggt
      301 cttgtcttgg gaacaactga actagttaaa gcacaagatg aaatttctcc aagcagccca
      361 cttgaaacag cgataagctc cgtacaagta ggtgacaaag tagcttcggg gaatactttt
      421 caggagaatc ctggttatac gaaaaattat aacttttcgg atttacaatt tagtcctcaa
      481 gaactaactg gagacacact aaaaggcaat acaattggat ttgaagttta cggaaaacat
      541 aatattgcag catcaactaa aaactgggaa atccgtcttc aattagatga acgattagct
      601 aaatatgtag aaaaaattca ggtcgatcca aagaaaggaa ttggatcttc tagacggacc
      661 tttgtaagaa ttaatgattc gctgggtaga cctacaaata tttggaaggt caattatatt
      721 cgtgcaagtg atggattatt tgctggggca gaaacaaccg atacgcaaac cgctcctaat
      781 ggggtaatta cgtttgaaaa gagcctagat gaaattttta aagagatagg catagacaat
      841 ctcaagaccg atcgtttaat gtatcgaatc tacttggtaa gtcatcaaga cgatgataaa
      901 attgtaccgg gtattgatag tacgggctac tttttaacag attcagatga tttctataat
      961 agcttagatg tatctgaaaa taatcctgac cagtttaaac atggatcagt aagtgcaaaa
     1021 tatgagaaac ctaatactca aacaaaagat ggttcaggat ccacaggtgc taatggagct
     1081 ataatcttag atcataagtt aacgaaaaat tataatttct catattcatc ttccgcaaag
     1141 ggcacgcctt ggtatgctaa ttacaaaatt gatgaacgat tagttccata tgtagcgggt
     1201 atacagatgc acatggttca ggctgacaaa gtaacatatg atgtttcgtt tgaatccgga
     1261 acaaaagtag ctgatttagc aatcgagcga cgggaaggtc atgaaaatta tggcataggt
     1321 tcaataacta ataatgattt aactaaactt atcgattttg ctaatgcaag tccacgtcca
     1381 gtcgttattc gatatgtttt acaattgact aagccattag atgaaatctt agaagatatg
     1441 aaagcaacag cacaagttga agaaaataaa ccatttggtg aagatttcat ctttgattct
     1501 tggttgtcgg atacgaataa aaaattaatc cagaacactt atggaacagg ttattattat
     1561 ttgcaagata ttgatggtga tggaaaccct gacgataaag aagagagcgg agacacgaat
     1621 ccatatatcg ggaaacctga attagaagaa gtatatgatg ttgacacaac agttaagggg
     1681 aaagtattca tccacgagtt agcgggaaca ggtcacaaag cccaacttgt tgataaagaa
     1741 ggtactgtat tagcagaaaa aactatcgct ccaaatgaaa aagatggggc tccaatttca
     1801 gatactgtag aatttgaatt tacgggtgta gattcaagta aattaatcgc gaaagatgaa
     1861 ttaaaaatcc aaatcgtttc tccaggtttt gataaaccag aagaaggttc aaccgttatt
     1921 aaggaatcac caaaagcggt tgataaacaa accgtggtag ttggatttaa accagatgct
     1981 aaagaatcaa ttcggaataa taaaaactta cctgaagatg cagagtattc atggaaaaca
     2041 gagcctgata cttctaacgt tactgatagt acgaaaggta ttgtaactgt taagatcgga
     2101 aatcgaactt tcgacgtgga tgtagagttt gctgtaaaag cttctcaagc tatggaaaat
     2161 gatgcaacat acgtacctat aacaacaact ccagaaacga cagttcaaag tggaaaacct
     2221 acatttgata aaccagatgt tcctttagct aaagatgcct tttcaatttc agatgtctat
     2281 aataagtact tcggtaatgc aagtgttgat gcaaacactg gtattgttac attcactcca
     2341 gctaaaggtg taggagaatc ggagccgatt actggaacaa ttcctattaa aattgtttac
     2401 caagatggtt ctgtaggcac gaccgattta gcagtaactg taagtaaaga tatttatgaa
     2461 aatccaggag aaaacattcc tgcaggctac cacaaagtaa ccttcaccgc agg
//
LOCUS       DASJSZ010000167         2441 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.174, whole genome shotgun sequence.
ACCESSION   DASJSZ010000167 DASJSZ010000000
VERSION     DASJSZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.174"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            138..2291
                     /locus_tag="U4B03_002808"
     CDS             138..2291
                     /locus_tag="U4B03_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN2069596.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          833 a          427 c          541 g          640 t
ORIGIN      
        1 gtgattgctg tgaaacaaaa agttgatttt atcttgaaat taggtaaaga atttcttgct
       61 ccgcccattt tttctttttt attaagaagt ttttgggtac aatgaagcat gaatgtaaat
      121 aggcggaagg ataggaaatg atgaaagatt ttatgaaaaa aatattaaat acttcttggt
      181 tagaaaaaat aagaaaaggt gcacaagaac ctaagaacaa aacgtttcgc cggtcccatg
      241 ttccttttcg cttaaatttt ctttttttca ttatatttac gttatttgtt gcgttgattg
      301 ctagattggg ttatttgcag atcgtgaacg gcgaagagat ggaggcgcga gtgaagtcta
      361 cttctacgat tacggtccaa gaaagtactc ctcgcggaat gatctacgac tccacaggaa
      421 aagctttagt tacgaataaa gcgaatcagg ccatcacata tacgagagga acacaagtta
      481 cggctaaaga tttgcttgaa atcgcaacga agctgaactc gctgatcgat gttccggtag
      541 atgaaaatct gacagataga gataaaaaag atttttggct ggctgatccc aaacatttga
      601 aagaagccac aaaaagactt tcggcaaaag aaaagcaatt agggaacagc gaacaatata
      661 gtgcgatggt caataaagtc acagctgatg agattaattt aaacgaggag caattaaaaa
      721 ttgctaccat ctttaaacgg atgaatagtg catatgaatt aaacactgtc tatatcaaaa
      781 actctggtgt tactgatcaa gagttagctg ttgtggcaga acgcgcttct gaacttccag
      841 gagtttcgac tggcacagac tggacacgag aatataatgc agcaagttca ctgaaaagta
      901 ttcttggcag tgtgacgact gaaaagcaag gacttccagc agatgaagca gagaaatacc
      961 tagcaaaagg ttattctcgg aatgaccgtg taggacaaag ttatttggaa aaacaatatg
     1021 aagatgtcct gcaaggaacc aaaacccaat atgaagtttc ccttgataat gaaggaaatg
     1081 tttcgaatca aaaagaaatc ttctctgggg aaaaaggttc taatttaatg ttatctatga
     1141 atgcagaatt ccaatcaaaa gtagaagaaa ttttgaaaag gaattatcaa acattgatca
     1201 acaacggcaa agcacagtat tcgcctggtg cttatgcagt ggctatgaat cctcagacag
     1261 gagaagtgct agcaatgact ggtttcagtc atgaacaagg ttccaaagaa atcaccgaga
     1321 atgctttagg cacgattact agcgcctttg atccagggtc tgtagtcaag gctggtactt
     1381 tgacagcagg ctgggcaagt aacgcgatca gcggaaatca agttttgatt gatgagccga
     1441 ttcgtctgca aggtgcttca gaaaaaagct ctgtctttaa tcgaagcgga caagttgctc
     1501 ttgatgcagt caaagcatcg gaattgtctt ctaatacgta tatgatcaaa gtggcgttaa
     1561 agatgttagg actagactat acacctggaa tgggactacc tagtttagat gaagaagcta
     1621 aagcttatca gcaattgaga gactctttca aagagtttgg tcttggtaca acgactggga
     1681 tcgatttgcc gaatgaatct cctgggatct cacgttctgt tgattatatg aagaagttca
     1741 atgcggataa tggcaaagaa tggtatgctc caggtaactt taccgatttg gcatttggac
     1801 agtttgacac atatacacca atccaattag ctcaatatgc ttcaacagtc gctaatggcg
     1861 ggaaacgagt acagccgcac ctagtcaaag cgatctatgg caatgacgaa aatggaaact
     1921 tgggtgaagt gaagaaagaa attggcacaa cagtagaaaa tacggtcaat atttctgcag
     1981 aaaatatggc gatcctccgt gaaggattcc accaagttgt ccatggtacc gatccttaca
     2041 caacagccaa accattagcc agtgcgaaaa tggacctttc cgcaaaaaca gggactgcag
     2101 aaacggttgc ggaaggtcat ccagatattg cgacagtcaa tagtaatatc gtggcatacg
     2161 gaccaacaga caatccggaa attgccatca gtgttgtctt gccgaactta ttagacgaac
     2221 aagaccacat aaacttgacg attgctaaag aaataatgga cgcgtattat gatatgttca
     2281 tggcaaaata aaaaagaggt cgtgaaaaag ccgcttagtt tcgagtatga agaaaaattt
     2341 tgtgaaaata gcttctcaca tttttgctta aatttgactt cataccgaga aactggcttt
     2401 tgaacaccgt ttactcggca aaaaaggttg tgacaaaaat t
//
LOCUS       DASJSZ010000168         2398 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.175, whole genome shotgun sequence.
ACCESSION   DASJSZ010000168 DASJSZ010000000
VERSION     DASJSZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.175"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            207..2069
                     /locus_tag="U4B03_002809"
     CDS             207..2069
                     /locus_tag="U4B03_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2069597.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="U4B03_002810"
     CDS             2069..>2398
                     /locus_tag="U4B03_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2069598.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DASJSZ010000169         2385 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.176, whole genome shotgun sequence.
ACCESSION   DASJSZ010000169 DASJSZ010000000
VERSION     DASJSZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.176"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            306..560
                     /locus_tag="U4B03_002811"
     CDS             306..560
                     /locus_tag="U4B03_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2069599.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            561..878
                     /locus_tag="U4B03_002812"
     CDS             561..878
                     /locus_tag="U4B03_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2069600.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1437..2372
                     /locus_tag="U4B03_002813"
     CDS             1437..2372
                     /locus_tag="U4B03_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069601.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          853 a          338 c          453 g          741 t
ORIGIN      
        1 aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc ataccgaacg
       61 gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt aaatggatgt
      121 taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta tatgttttca
      181 gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct taagaaacac
      241 cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa aaggagtgtg
      301 ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga gtgatccttc
      361 caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa ggcaacttat
      421 tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt gaagaaagtt
      481 tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa gctttcggaa
      541 aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa agttttcaga
      601 ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct ccagccagca
      661 taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat tttccaacta
      721 tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg atcgtgatcg
      781 gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata cttgtaggac
      841 gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga aaaagtcgct
      901 catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt cggttactcg
      961 aagattgata tataagactt agtacctttt tattttgctc catgttatgg ccgaaggtct
     1021 aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg gggatcgctt
     1081 ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc tctattccca
     1141 aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc aaaattgacg
     1201 taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt taaaagagga
     1261 agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa aatatacatc
     1321 cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa aggaatggga
     1381 taaaatatag gtacaataac tatttgatgc taaatataat taatggagga gagatagtga
     1441 acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt actactttca
     1501 aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa ggatacaagt
     1561 ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt ctttctgaga
     1621 gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt gatatatata
     1681 cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc agtgaatatc
     1741 tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta ctaccatatt
     1801 tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca ggtaatcaag
     1861 gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg atgggttacg
     1921 gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct gggcgttcat
     1981 tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag ttagaaaaca
     2041 tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt gaagttttga
     2101 aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata aaagcacatt
     2161 ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga gggaaaatca
     2221 atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta atgataacag
     2281 atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata aaacttgtta
     2341 ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DASJSZ010000170         2308 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.177, whole genome shotgun sequence.
ACCESSION   DASJSZ010000170 DASJSZ010000000
VERSION     DASJSZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.177"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..617
                     /locus_tag="U4B03_002814"
     CDS             99..617
                     /locus_tag="U4B03_002814"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2069602.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            745..1428
                     /locus_tag="U4B03_002815"
     CDS             745..1428
                     /locus_tag="U4B03_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEN2069603.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1737..1810
                     /locus_tag="U4B03_002816"
     tRNA            1737..1810
                     /locus_tag="U4B03_002816"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1771..1773,aa:Arg,seq:tct)
     gene            1969..>2308
                     /locus_tag="U4B03_002817"
     CDS             1969..>2308
                     /locus_tag="U4B03_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEN2069604.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLA"
BASE COUNT          779 a          351 c          465 g          713 t
ORIGIN      
        1 taattattaa agggaaacat ttttacagct ataatttgaa aaaatttatc gttattatat
       61 gattaagaag gattgaataa agaaagaagt tgaaaacgat acctgattac aaaaaaataa
      121 cttttataag ttctataacg ttgattatat ttttaattct tattcttctc gtcgtcttgg
      181 ttccccgaat ttttggtttg actccttata ttattgagga caaagaaatg agtccatatt
      241 atgcacaagg aaatctgata tatgtacgac cgaaaaatac aaatgagatt ttagtcggag
      301 atattattac atattatgaa aattcaggaa aaaaaatctc gacgcgtcgc gtaatagccg
      361 ttgaaaacaa ccacgaagat ttttatacaa aagctgatac aaagacttat attgagcctg
      421 gagtagtaaa aaagagaaat attatcggta gtcccatctt tcagattcca tatattggac
      481 tagtcctttc aaaaacggca tttgcatgga ttcaggggtt attctttacg attgcttttt
      541 gcctaacagg aatcacagca tggaatacct ttgtagagtt gaaaaagaag cggagacgaa
      601 cacagaaatt tttgtaagaa tgttaaaaac ataaatgtat tccagagatc caacgactct
      661 tctgaacatg aaacacgaga aaacactcat gcagcttgta gatttagtca gttgttcgta
      721 taatgtgtga agaggaggag tagtatgcgc aatgaaatga tgcatcgtcc tgtagtcaaa
      781 gaagaactat tggattttat gcgtacccag caaaaaaaat taccgggtga gctaggcaaa
      841 ctggaagaag aagctcatgt agcagaagta ccagttattc cccatgaaac agttgtattt
      901 ttgaaatttt tacttggaca gcttcagcct gaacgaatct tggagatcgg agcagctatc
      961 ggtttttcat caagtgtcat ggccacgaca atcggagaaa acgctcatgt tacgacaatt
     1021 gatcgtttcg atgtcatgat cgagaaagct aaaaaaaact atgaacgtct aggactgaca
     1081 gataaagtga ctttgcttga aggtcaagca gcagatatct tgccggagtt gtctggtccg
     1141 tatgacttta tttttatgga tagtgcaaaa tcaaaatata ttgaattttt acctgaatgc
     1201 ttacgtttgt taagaaaagg tggcgttttg atggtagatg acatcttcca aggaggaacg
     1261 atcttgctcc cagatgaaga gattccccgt ggcaaacgag ctatccatcg taaattaaat
     1321 gaatttcttc gtgttgtgat ggatcatccc gatttgacat cgacaatctt gccattaggt
     1381 gatggtgtga tcttaatgac aaaggaatca gaaacaatcg agttgtgata catgattatg
     1441 gcagcctgta gataatcgat tgaatcgagg gctcaattcg aacttgatgt aaaagtttct
     1501 atatttgtaa gtcaattaaa ggatacagga aatctaaata aacaggagta gcatgaagtc
     1561 aatgtagctc ctgttttcaa ttgataacct tagaacagct gaaggagaat gtctcgtttt
     1621 aaaagattga tttagtagtt gggccaaaat aattcattat ttgctaatta aataactatt
     1681 tcgtattgac agaagcaatt taattatcta taattattga tgttccgctg attcttgtca
     1741 tagtagctca gctggataga gcatccgcct tctaagcgga cggtcggggg ttcgaatccc
     1801 tcctgtgacg ctaaaaaacc gttagaaact gaggtttcta acggtttttc tttgtttttt
     1861 gtgccaatat tgtttgtttt caaaggcaaa taaaataaaa aaacatcctt atcgagtaca
     1921 attataagga gcaaaaaaat aaaactcatc agaaaagagg aagaagatat gacgatacta
     1981 tctagaattt tagtgactct agttgcaatt gaattttttt atatcatgta tatcgaaacg
     2041 gtgagaactg attctgacac gacaagtagg gtttttaaaa tgagtaaaga agagttaagc
     2101 agaaaatctg ttcaaacatt gtttaagaac caaggagtgt acaatggact attaggagtg
     2161 ggactacttt atggagctta tctttcttcc gcatctaaag aaatcacaag tatgctatta
     2221 atatctattt tttttgttgc attatatggc agtttgacta gcgatcgttg gattttactc
     2281 aaacaaggtg ggctagctct gttggcat
//
LOCUS       DASJSZ010000171         2296 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.178, whole genome shotgun sequence.
ACCESSION   DASJSZ010000171 DASJSZ010000000
VERSION     DASJSZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.178"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            130..1743
                     /locus_tag="U4B03_002818"
     CDS             130..1743
                     /locus_tag="U4B03_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2069605.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          718 a          408 c          447 g          723 t
ORIGIN      
        1 taggttttat ccacaaatta tttctctata ttttctgtct attcaaaata aacaataatt
       61 ttccggcaca attatttgct gttttttacc ataacatagt acgatcaacg tagaaaagga
      121 gatgttttta tgaaagttgt tgtcattggc ggtgtagctg gcggtccttc atttgccact
      181 cgtttccgtc gattgaatga agcgcacgaa atcattatct atgaacgcgg agaaaatatt
      241 tcttatgcaa gttgtgcttt gccttattat ttaggaggtg tgatcactga ccgtgactcg
      301 ctgatcgaac gtacaccaga aatattgaaa acaaaaaaca acatcgatgt ctttactaaa
      361 cacgaagtaa cagcaatcga tccttctact aagcgattaa cagttaaaga cctatccaca
      421 aatgaagaaa taaaaacaga ttacgataag ttgatcatct cttctggtgc tagaccagat
      481 tatccggata ttcccggagt ttttgaagca gaaaacggct ttgtcctccg tagtgtgacg
      541 gatgcggatc gaatcaaatc gttccttgaa gaaaaaaatc cacaacatgt cgtcattctt
      601 ggtgcaggtg ttatgggtct ggaattagct gagaatttca agcatcgcgg cttagacgtg
      661 actttaatcg atcaattgcc acaagtcgct ttcccttatg atccagaaat tgctaattta
      721 gtttatgaca aattgctgaa agaaggatta gccgttcatt tagaaacaag agttactgag
      781 atccgtgata aaggtcgaga aatagtatta tcagatggct ccgtcctttc tgctgatatg
      841 ctaatttttg ctgttggtgt ttctccgaag aatgaagtgg tgaaagcaac cggcatacaa
      901 ttatcagata caggtcagat cattgttgat gaccagttac aaaccaatct gccggacatc
      961 tatgcgattg gcgatattat cgaaacgact agtgtagtga ctggtcagcc gatccaaagt
     1021 atgctttcca gtgcggccaa tcgtcaagga catatgctgg cagatatttt aaatggtacg
     1081 cctatgcgct atcgcggata tattggtgca ggtgtcgcaa aaatctttga tcatacagca
     1141 agttatgcgg gaatgacaga acatgaatta aaagcatcag gcataacaaa ttataaaact
     1201 gtttttatca ctccctttga ccatgcctat ttctatccag aagctacaag attaaatcta
     1261 aagctgattt ttgatgcaga tagcggtcgt attttaggtg gacaagcatt tggagaaaaa
     1321 ggtgtcgata aacggatggg agaactttct gtagcaatca ccggaaactt gacagtcttt
     1381 gatttgcctg atttggagtt gccttactct ccaccgtatt ctactacccg tgacccgttg
     1441 aatatagctg gttatgtcgc aatcaatcaa atgacgaata tcgtagaaac gatcaaagca
     1501 agtgatatac ccgaaaacga tttgaaagaa gcgttctttt tagatatacg tgaacctaat
     1561 aaagcacctt ctggcagtat ctcggcaaca aaaaatattc caatgaatga gttacgagat
     1621 cgaatcaatg aaatcccaaa agataaaaaa gtttacatta ctttcagaag aggattgaat
     1681 acttatactt ctgcccgaat cttggcaggt ttgggtatca aagcgatttt gattgaagaa
     1741 taaaaaaacg accgcggtgg gtagccgcgg tctaggagtt atttttactt gaggagtaaa
     1801 aatgaaaaag tatatagggt tgttggttgg tatgtatata gaatagaaga taaatgtgaa
     1861 gaaattgtgc attatttctt cacatttatt aggttatagt ttaagaaagt ttaaacattg
     1921 cgcaatttaa ttattttagc tgtttctttt cataaaaaaa catgttgtga ctctgagaca
     1981 aatcatagtc catagtgatg tctctagagt aactacgcct aatattacga ggcatcatat
     2041 taatctatca gcactagtac ctaaactagc atccctttcg aatatttttt cttatataat
     2101 aaagtttgct ttcactcctc taatctcacc aaatcgactg gcttgcgttg tgtgatacta
     2161 ggaactgatt ggtttagaac gacttttcta ttgtaatcat atttttcgcc atcctcgttg
     2221 tcttctaatc cttcaatgaa tgatccctaa tgtgtgtatt aaataattta atttttttgt
     2281 tcctatttat ttaaca
//
LOCUS       DASJSZ010000172         2272 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.179, whole genome shotgun sequence.
ACCESSION   DASJSZ010000172 DASJSZ010000000
VERSION     DASJSZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.179"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            21..347
                     /locus_tag="U4B03_002819"
     CDS             21..347
                     /locus_tag="U4B03_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2069606.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            348..1097
                     /locus_tag="U4B03_002820"
     CDS             348..1097
                     /locus_tag="U4B03_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069607.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(1177..1632)
                     /locus_tag="U4B03_002821"
     CDS             complement(1177..1632)
                     /locus_tag="U4B03_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN2069608.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(2059..>2272)
                     /locus_tag="U4B03_002822"
     CDS             complement(2059..>2272)
                     /locus_tag="U4B03_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069609.1"
                     /translation="GYTICSFKKDRSIKAYTQNTEKGIIYFLHEEGYDTQTFMFTEKK
                     EFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT          725 a          340 c          434 g          773 t
ORIGIN      
        1 agtaatatgg aggaaagaaa atgatttcaa gtgatggaat acgcggatac aatgatgcga
       61 ttattttatc gatccttaac aaaaacgatt cttatggata tgaaatttct aaaacgattc
      121 gtgagaggac aaatgattcc tatgtgatca aagagacaac actttactcc gtttttgccc
      181 gtttagaaaa gaatcaactg attgtttcgt atcctggaga agtgactcat ggaaaaaagc
      241 ggacatatta taaaatcact gaaatagggc gtaatctttt agaagaaaaa aagcaagagt
      301 ggctgacgac gaaagacgta gtcgatcaat ttttggaggg aaattaaatg gatacaatcc
      361 gtaattattt agacagtctt tttatcggag tacctcaaag taccgagata gataagctga
      421 aaactgattt gttggcaaat atggaagacc attatcatga attgatggga gaaggaaaaa
      481 atgaacagga ggccatcggt acagtaattt cgacatttgg ttcaatcgat gaattattag
      541 aagaactaga tgtcgaaaaa aaacatcaag ctgacgagac ggaaacaaac acagcttcta
      601 tttatttaag cgaagcagaa aactactgga aagaatacag agcggcaagt ctccaagtgg
      661 catcaggtgt tctattcatt agcttatctt ttgcaagttt tcttttcttt tgttcagcag
      721 gatatgtgtt catgggtatt tcttgtctga ttttcggtat agcattagct gtcggttttt
      781 ttattgcttc tggtatgaaa atcacacgtt taaatcactt tttgcaccat cggaaaatac
      841 ctgaaaaagt attagcagaa gcaaaagaaa aagaagaaga gtatcagcga agttttggct
      901 tctcactgat tgcaggaatt ggattatgta ttttttcact tttcccactt ttggcttctt
      961 taatgtggta tatggatggt agtatcggtg cctctatatt ttttgttacg gtaggaacgg
     1021 gtgtcttctt gatcatttat ggcagtctag tccgtcattc ttatcgtcaa ttcactcaaa
     1081 gcgcttatta ctggtgaatg tattcagatt tgtaaaaata ttctatttat ccggcgtaga
     1141 aaaaaagact gtgacacgct tttgtcacag tcttttttat atataataat ttaaaaattc
     1201 atcatctaaa tccgtagaat tttctagatt gcttacccat gtaaaatcgt tatagacaga
     1261 tttcagttga cttctgcgcc attgtacagg gataaaaaga cgcatgctat cttcattgaa
     1321 tccgactaat aatttctttc cgtcagtaat gatgggacgt tgtaacaaag taaaatgcga
     1381 gacgatcaga tcgacaagct ctgaggtcgt acattggtcg atttggtctt tcaaattttt
     1441 ataatcaatc gagcgtttag ctaatagatc gtcaaatccg ttttctgtta atgacaataa
     1501 ataaaatatc tctttccttg ttaaaggctg tttaggtgtg aaaattcgtt ctctgtaagg
     1561 catttgatgg tctttcagcc agtttgccat cagatggtaa ctatgtgtgc gagaagaata
     1621 gatcgtaagc ataaaaaaac tccttaaata gtccattaat ttgttagatc tgttgagata
     1681 aataataggt aaggagctgt gcataagcat tgatcaaaac aacccaaaca tcattagaaa
     1741 taactttcta aagtcttaaa cttgcgacga gaagcaacta gactaataga aaaaatgtct
     1801 tagccatttg gcttcctgtt tgtttttttg cttatgctgc agctcctcgg gaaagggttc
     1861 taacaatttg aagaaggttg atgattgatc tagtttaaat tgtgtgaaat ttgaaatctg
     1921 gtgtttttac tagatgaatc acctaacaac tatgattata acttatagaa cttctgtttt
     1981 gcattcggtt ttgttatagt tgttataaag ttttatttta ttacagaaca tttttaatta
     2041 ataaagaaca gttagaagtt aaaattgctg attcacatag agtttatgac ttcttggaaa
     2101 ttctttttca atcagttttt tcatctgttt atgaaattct tttttttcag taaacataaa
     2161 ggtttgggta tcgtaacctt cttcatgtag gaaatagatt attccttttt ctgtgttttg
     2221 cgtataagct tttatactcc tgtctttttt gaaagaacag atcgtatagc ca
//
LOCUS       DASJSZ010000173         2256 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.180, whole genome shotgun sequence.
ACCESSION   DASJSZ010000173 DASJSZ010000000
VERSION     DASJSZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.180"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..261
                     /locus_tag="U4B03_002823"
     CDS             <1..261
                     /locus_tag="U4B03_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HEN2069610.1"
                     /translation="FIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMF
                     SLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            373..1824
                     /locus_tag="U4B03_002824"
     CDS             373..1824
                     /locus_tag="U4B03_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN2069611.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT          848 a          342 c          407 g          659 t
ORIGIN      
        1 ttcattagac ctgctttttt gagaaaaaat agtcgtgtaa aattaagaat tagctttgaa
       61 aaaaatacaa agagaatagt aggtgtacaa ctttgtagtc aagaagattt atctggacta
      121 ttatcaatgt tttctcttgc tatagaagaa caaattacat tagacaaatt gaaaatgtta
      181 gattatttat ttcatcctag ttttagccaa ccctataatt ttattttaaa ggcagtaaca
      241 ggattaaaca ttgaaacata atcgttacta aatgaaatca agaaatatac tgtattactc
      301 aattcattga aaatatttat cataattttt aatagaaaac actattaaag agaaaaaaga
      361 gaggtaaatc aaatgaaaaa atctttaaaa cttgcatcag cagttatatt aatgtcccaa
      421 ctccttatag caacaccttt ccatgtgttg gcaagcgaaa attcaacata caaaattgca
      481 aaagaaagaa ccgagacgac tgaaatgagt aattccgatg taacagcaga gagtagtgaa
      541 cttttgacaa cttcatcaag tgaaagtagt acgacttcaa atgccgagtc ttcaacaact
      601 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      661 agtagtacga cttcaaatgc cgagtcttca acaacttcgt caagtgaaag tagtacgatt
      721 tcaagtaccg aatcttcaac aacttcaagt agtacaagtg aaataacttc ttcgaaagtg
      781 acaaaaccaa accatataga atcttcaaaa aaggaagcgc caaaaacgga aggacaaaca
      841 aaaacttttt cgacaccaca gcaaatagaa tcaacagaat ctttccaatc acaaccaata
      901 ctatctggta atgaagaaat tcattttgac aaaaatgaat ctgtagaaaa ttttattatg
      961 aaaattggtg agtctgcgag aaaaattggt cagaaatatg atttatatgc ttctgttatg
     1021 attgcacaag cgatcttaga atctgccagt ggtcaaagcc agttagctca ggcaccgaat
     1081 tacaatttat ttgggataaa aggaacttat aatggaaact ttgtcattat ggtaacaaac
     1141 gaagatttgg gtaatggaac gctttatacg acccaatcaa aatttagagt gtatgaaaac
     1201 tatgaggaaa gctttgaaga ttatgccaaa ttattaacta agggaattag tggaaataaa
     1261 gatttttatg caggagcatt aaaagctaat agtaaaacat atcgagaagc aactaaattc
     1321 cttacaggac ggtatgcaac agatacacaa tactatttga agctaaatga attaattaaa
     1381 acctacgatt taaccaacta tgataaagaa gttaaaggtc cacaaatgag taagactgga
     1441 taccaagtgc ctattcataa ttattcaata accagtttgt ttggaaatcg tggaaatgaa
     1501 tttcaccgag gacttgatat ggcagctaca cagggtgagc cgattcatgc agttaaagct
     1561 ggtaaggttg ttaaagcaga gtttcactac tcttggggaa attacgtggt gatacaacac
     1621 gaagatggat caaccgcgtt atatgctcac cagcaacaat acctagtaaa aactggagat
     1681 aatgttttgc aaggacaaat aattggctat gtaggaagta ctgggaatag tactggccca
     1741 caccttcatt tggaaatttg caaagatagt agtctttctc agagcatgtt agttgatccg
     1801 aaaaccttat tattcggaga ctaaaaaagg taatcaatct gacaaagtaa caagaaaatg
     1861 gttggattct tagacctttg atcaggatat tgagactgga attgatatct cgattacgat
     1921 gggtttgaca aataaaacaa gtccattatc gaatatcgag tgtattattg ccgtctttat
     1981 gctcatattt tgagcaggtt tcactagatg aaaacaattt atctatcttg aggatttcgt
     2041 gtacgtatcg ttcagtcttg tattgtaaat tagtcataaa atcagactaa gaaaaattgg
     2101 aaatattttt ttctgattta ttaaaagtta attgaaatta tatggtactt ctttcttggg
     2161 agggtaggtg cagtgaagga aagatatggt caccttactg ttgttgagga gattactcat
     2221 aatggatata aaaaatatcg ttgcctatgt gactgt
//
LOCUS       DASJSZ010000174         2206 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.181, whole genome shotgun sequence.
ACCESSION   DASJSZ010000174 DASJSZ010000000
VERSION     DASJSZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.181"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            153..>2206
                     /gene="acm"
                     /locus_tag="U4B03_002825"
     CDS             153..>2206
                     /gene="acm"
                     /locus_tag="U4B03_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEN2069612.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KS"
BASE COUNT          867 a          338 c          435 g          566 t
ORIGIN      
        1 ttggcatatt tattttttat aaatatttgt aaaataacta tcaatttttt agttaaaaga
       61 ttggaaatga gtgggggagg aattgaagaa aggatttttg agagatgata tagtaggttt
      121 tatttttagt taaaataggg gagatagaaa aaatgaaaaa gtgtagaaat attattttat
      181 caatgttatt tattattact aatataacat cactgattcc tgttcatgtc tatgcggatg
      241 caggcagaga tatcagcagt aatgtcactt cgttgaccgt ggacccaacg aatattacag
      301 atggaggaaa cataaaagta aaattctcgt ttgacgaaaa aaaacaaaat atccaaccag
      361 gtgattatct ttggatcaat tggccaagtg aaggaaatat tcgcggtgaa ggattccaaa
      421 aagaaatccc attaatgatt gaaaataaaa atgttggaac tttaacggtc agaaaagatt
      481 ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga ctcagtagaa ggttgggggc
      541 aatttgagat acaggccaga aacgtaaccg ataccggtga agaaaatacg ggaccattca
      601 ccgtgacgag cggtgataaa acagctactg taaatgtgac aaagccagct tctggttctt
      661 cttctagcgt tttttattat aaaacaggag acatgctacc tgaagatact aagcacattc
      721 gatggttttt gaatattaac aataacggca cttatgttga acaaccagtt aaaatcagtg
      781 atgaaatcca aagtggacaa agattagatc caagtacgtt tgaaattaat caaatacact
      841 taggtgaaca gaaggtttat agaggagaag agggaatcca acaattttta caagatttcc
      901 ccagcgctac atttaatttt tcagtaactg ataattacat tgaaataact ataccaaaaa
      961 actttgtcaa tcttaggaaa ataatggtga gttataagac gatcatagaa aacccagaac
     1021 aaataaattt tgagaatcat tcggaagctt ggtttaaaga atttaataaa cctgctgttg
     1081 atggagaaag tttcaatcat acagttaaaa atatttcagc ttctggtgga gtcaacggta
     1141 cagttagagg cgaattgaag attttcaaat atatcaatga cacagagatt ggtatcccaa
     1201 atgtgacttt cgaattaaga cgagcagatg aacagcctat tcaaggacag tctagtatcc
     1261 tgttgacttc gaatgaacag ggagaagcta gtatcaaagg cctgcaagta ggagactatg
     1321 ttgtaaaaga aaaagaagca cccaattgga ttgattttga tccactaagt tcaaatgaac
     1381 tcaaattctc cataaacgaa aatgatacag agggagtcag tttacctatt tataacaaga
     1441 aaaaggttac gaacatcaca gcaactaaaa tttggaacgg tggaaccact ccaagaccga
     1501 gtatttattt taaattattc agagcaagta caaacaattg ggaaccagtt cctgatgcag
     1561 aaacaaaacg attagacaat ggaataactt cagtgacttg ggaagatatc caacagtatg
     1621 atgatagtgg aaacgaatat acgtttaaag tgcaagaagt agatcaaaac ggaaatgatt
     1681 atgtcccttc aggttatcaa aaaattgaaa acgggctagt cgttacaaat gagaataaag
     1741 aagtcatttc agtctcagga caaaaaacat gggaagacaa tgagaaccaa gacggcaaac
     1801 gaccgacgac gatcacagta aatctattag ccgacggtca accgatccaa cacaaagaag
     1861 taagtgaaaa agatgattgg cgctatcgtt tcacaaatct gccaaaatac agagacgggc
     1921 aagaaatcat ctatactgta acggaagaca acgtgcccga atacagtacg acgattgacg
     1981 ggtataacat caaaaacagt tatacaccgg aaaaaacaaa tatatctata ccaggtagcc
     2041 atataacacc gtggaaaaat tttccagaga agacagagag agagaaaatt agaaatctat
     2101 tctctcaaaa attgcaaata tttgctcata caggagaatc cagcgaacaa agaagcaatt
     2161 atattagtaa gagagctgta cctaaacaga taacaaataa atcaaa
//
LOCUS       DASJSZ010000175         2201 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.182, whole genome shotgun sequence.
ACCESSION   DASJSZ010000175 DASJSZ010000000
VERSION     DASJSZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.182"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..555
                     /locus_tag="U4B03_002826"
     CDS             <1..555
                     /locus_tag="U4B03_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEN2069613.1"
                     /translation="EKKKYVLQVFHPVVNELENIEKQILALINSMEQQSQPIICILPN
                     SDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIP
                     VINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRR
                     ITKTLNQILNDGEDIKWIQKQIAY"
     gene            528..1205
                     /locus_tag="U4B03_002827"
     CDS             528..1205
                     /locus_tag="U4B03_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN2069614.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1156..>2201
                     /locus_tag="U4B03_002828"
     CDS             1156..>2201
                     /locus_tag="U4B03_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN2069615.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGV"
BASE COUNT          826 a          298 c          432 g          645 t
ORIGIN      
        1 gaaaagaaaa aatacgtttt gcaagtattt catccagttg taaacgaact agaaaatata
       61 gaaaaacaaa ttttagcttt gatcaattca atggaacaac aatctcaacc aattatttgc
      121 attttaccta atagtgatgc tggtagcgaa attatcagaa aaatgtacca gaaatattta
      181 aaaacggata ttatttatat tacgaattta gagccggagg aatacctgac tatattaaaa
      241 gagtgcaaat ttatgatcgg aaattcttct tctgggataa ttgaagctgc atcattcaaa
      301 ataccagtaa taaatttagg aaccagacaa aatggacgag aacaatctaa aaatattata
      361 aatgcaaatc taaatgaaaa tgaaattgaa actgctttga ataaaataca agatcctcag
      421 tttataaaaa gcttgaaaaa tgttaagaac atctacggtg acggcaacag ttctagaaga
      481 ataacaaaaa cattgaatca aatattgaat gatggggaag atattaagtg gattcaaaaa
      541 caaatagctt attaaaatat actagtgatc catacattat tattggggct ggcggacacg
      601 caaaagtcat tattgaaacc ttaagattag aaaataagtt aatatatgga ttttttgatg
      661 ataagcaaaa taaaatgcaa gatctgaaat atcttgggaa aataaacgaa ttgaatagct
      721 gggcggatag caaaaaaaaa atctataaat ttattgtagc tattggtgac tgtgtagtaa
      781 gaaaaaagat tataaaaaaa ttgaatatgc caagtgagca atatggaata gctgttcatc
      841 caagtgctat agtggcaaaa gaaatttcaa ttggttatgg aactgttatt tttgcgaatg
      901 ctgtaattaa cccagatgct gtaattggag agcatgctat aataaacaca ggtagcattg
      961 ttgagcatga ttgtagaatt aataactatg ttcatttatc tccaggtgta tgcttagcgg
     1021 gtggagtaca tgtaggtgta ggaactcaaa taggaattgg ttctcaatgt atccagatga
     1081 agaaaattgg atcttggtgt atgataggcg cagggagcac tattgttaag gatattccct
     1141 cacattcctt agcttatggc aatccagcta aaattaagaa agaaggaatt aattttgaat
     1201 tctagaattt tattatcaag tcctcatatg ggaggaacag aagaaaaata tgttgctaag
     1261 gcttttaaag ataattggat agcgccatta ggtgaaaatg tggataaatt tgaagaaagt
     1321 attacaaaat atactggttc taagggcgga gcttctgcgt taagttcagg tacagctgcc
     1381 atacatcttg ccttaagact gcttaatgtt ggaaaaaacg atattgtctt tgctccttca
     1441 tttactttta tagctacagt aaatcctatt ttatatcaag gggctgtacc agtgttcatt
     1501 gactcagaac ctaatacatg gaatatgagc cctgttgcac tagagagcgc attagaagaa
     1561 gctgaaagaa acaaccaatt acctaaggca atcatcgtag tgaatttata tggtcaaatg
     1621 gctgattatg aacgaatttt agctattgca aaccaattta atgttccagt gatagaagat
     1681 gcagcggaat cgttaggttc tacttataag ggaatttcgt caggcttatt tgggacaatt
     1741 gggatctatt catttaacgg taataagatc atcacaacct caggtggagg agctctagtg
     1801 tctagtgata aaaaatatgt tgagcattca agatttttag caacacaagc aagagatcaa
     1861 gcaccgtatt atcaacatag cgaaataggt tataattacc gtctaagcaa tatttctgcc
     1921 ggtatcggac gtggacaaat ggaagtattg aatgatagag tagctaaacg aagagaaatt
     1981 ttcaatcgat ataaggaagc tttttctgga attgatagaa ttgaaatgat gccagaagtg
     2041 gatggcatgg tgccaaatcg ttggttgtct actttaacga tcaatgatta taattctatt
     2101 gatcaagtag ttaacgtaat aaataaacta aatgaaaaaa acattgaaac aagagctttg
     2161 tggaaaccac tacatttgca accagtattt caaggggtaa a
//
LOCUS       DASJSZ010000176         2168 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.183, whole genome shotgun sequence.
ACCESSION   DASJSZ010000176 DASJSZ010000000
VERSION     DASJSZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.183"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="U4B03_002829"
     CDS             131..505
                     /locus_tag="U4B03_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2069616.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="U4B03_002830"
     CDS             complement(619..1248)
                     /locus_tag="U4B03_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2069617.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="U4B03_002831"
     tRNA            complement(1382..1454)
                     /locus_tag="U4B03_002831"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="U4B03_002832"
     tRNA            1668..1741
                     /locus_tag="U4B03_002832"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="U4B03_002833"
     CDS             complement(1879..2043)
                     /locus_tag="U4B03_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069618.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
BASE COUNT          732 a          345 c          362 g          729 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcat
//
LOCUS       DASJSZ010000177         2164 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.184, whole genome shotgun sequence.
ACCESSION   DASJSZ010000177 DASJSZ010000000
VERSION     DASJSZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.184"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            91..540
                     /locus_tag="U4B03_002834"
     CDS             91..540
                     /locus_tag="U4B03_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069619.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            540..1034
                     /locus_tag="U4B03_002835"
     CDS             540..1034
                     /locus_tag="U4B03_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069620.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            1048..1479
                     /locus_tag="U4B03_002836"
     CDS             1048..1479
                     /locus_tag="U4B03_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069621.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            1481..1618
                     /locus_tag="U4B03_002837"
     CDS             1481..1618
                     /locus_tag="U4B03_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN2069622.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            1655..1948
                     /locus_tag="U4B03_002838"
     CDS             1655..1948
                     /locus_tag="U4B03_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069623.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1945..>2164
                     /locus_tag="U4B03_002839"
     CDS             1945..>2164
                     /locus_tag="U4B03_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN2069624.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT          798 a          326 c          465 g          575 t
ORIGIN      
        1 aaattccaag aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt
       61 acttttgaaa aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc
      121 aatgtacagg tttatccaca aactcattgg aatgctatag aaggcaaacc aacaacggtg
      181 aagggagata aaggcgatcc tggccaagca gcaacaatta ccataggaac cgtttctagt
      241 ggttcaaccg cttctgttac gaatgtagga acatcatctg cagctagatt taattttgtt
      301 ttaccaaagg gagataaagg tgatcctgga ataaatgcca caactacagc tgtagctaca
      361 actacagcaa atgggttgat gtcgtccaca gataaaacta agttggatgg aattgcagct
      421 ggagcacaga aaaatccagg taatgctaca actacgacag caggtttaat gtcagcaacc
      481 gataaagtga aacttgatgg attagcgaat attacatttg agaaagtggg gacggtttaa
      541 tggctgatat tgttcaatta aaagaagacg gagttgctaa atacttaaaa acgcatgtag
      601 aagctattga tggtaaagaa gcattggtgc agacagatgg agaccaagcc attgcaggac
      661 ataagaattt ttcaggttct gtaacaatca ataataaacg tgttttgacg actgatgata
      721 acagatatga agtggtaaat ctggttgtca ctaacggtaa tacagggaca gcaaagcttt
      781 atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa agtagtggcg
      841 ggaatgattc ggttatttta actgttccag aaggctatcg gacaccaatt agttttgaac
      901 aactggttgg gtcaatagac cgttctactt tgaacagtgc tcaactatct attggcgcag
      961 acggagccat taaatggaga agaaattcaa gttatggatc agcttattca tttgttatca
     1021 cttattcaat ttaagggagg aaatctaatg aaagtagttt acaaatcaat caagccttac
     1081 ggattcgagc aaatcatttt gaacaatcaa gaaaatatcc ctgaaaactg tacagagatt
     1141 aaaccaccag ttcctaactg gagaccaaga tttgattttg ataaaaaaca gtgggttgag
     1201 ttagctactg aagaagagaa aaaagggaat gctgttgata gcgtagatga attggctaat
     1261 attaaggcat tatacgaaac gctgaaggca gaaaatgatg aattgaaaca gctaaattct
     1321 aaagcaatgc taaacaatgt agcaatcaag caagaaaatg tcttattgaa agaaaagtca
     1381 gaaagtttag cgcagttgaa ttcaaaaacg atgcttgctt ctgtacaaaa taccaaggaa
     1441 atcgaagaaa tcaaaaaaca attacaaggt gggaagtaac atgtattcat atgaagatat
     1501 caaactaatg tatgactggg gctgtttcac taacgaacaa gtaatggttt tcgttccatt
     1561 atgcattaca gacgaagaag cagataaaat cattaataaa gataagagcg catcttaatt
     1621 gatgcgtttt tatttaaggt aaaggagttg tcacatgatt aatttagggg aatggggaat
     1681 gatagcagga tcaataaccg ctatcgtttc tttgatttta ttagtaatta gaccgattgc
     1741 tgcatctttc tcgaaaatta ctgagactct ttcaaaagta agccacaatt tagatttgct
     1801 gactaaagat ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa
     1861 acacgatgaa agattagata cacatacaga aaaattggtg gaacacacgc aacaaatcaa
     1921 aactttgttt agggagagaa gaaaatgaat aataaaacgt tcgaagtact aaaatggttc
     1981 gcactggtaa ttattcccgc actagctact ttcgtggggt tagttggtaa agcgctcaat
     2041 tggcagtaca cagatatctg tgttgtcatc attactggtt ttggcgcgtt tttagggagt
     2101 gtgttgggtg tatcaaatcg aacctacaaa atgttctcgg ctgaaagcga agaaggagga
     2161 aaca
//
LOCUS       DASJSZ010000178         2158 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.185, whole genome shotgun sequence.
ACCESSION   DASJSZ010000178 DASJSZ010000000
VERSION     DASJSZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.185"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(33..458)
                     /locus_tag="U4B03_002840"
                     /pseudo
     CDS             complement(33..458)
                     /locus_tag="U4B03_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            complement(567..1598)
                     /locus_tag="U4B03_002841"
     CDS             complement(567..1598)
                     /locus_tag="U4B03_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2069625.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          679 a          347 c          369 g          763 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg ttaggaatct gttttgattt tgttccattt
       61 gaaatttctc tcactcgctt ttttgtctca tcatcaaata aaatgaaagg gtcttctaaa
      121 cgatcgataa atacttccat tgttgttgtt ggtagatgat aagaagtcat gacaggcact
      181 gcaccaaggt aagacacaga aacagctaga aggtatgtgt caaaagcgga actcttataa
      241 ataataactt tgtctccgga tttcactcca agatgagcca attgataagc ccgtttcaac
      301 acactttcgc agcatttttt gtaagttgtt tcaagtccta attctgggaa agctgaaagt
      361 ggctcatcaa agataatcgg ggtctcggaa aagttagctg cagcttcttg aaaattcgta
      421 aataagttca aaggggaata cgttatggtc tccatcattc acactccatt atcttttgtt
      481 aagtaatata caaattcaag ataatcgtaa cataatcctt tatataataa catagtttat
      541 tgtgaaaaaa agatcataca tttgttttat atggaagaaa cccgaacgaa tgccgctaat
      601 tcgtgataag aaaaatcaac catcttttcc cagatatctt gaaaagaatg ggatgagagg
      661 tatcgattac cacataaata atttaccgcg tcagcatcat ctaaatatag ttctgcataa
      721 taacgatatg tttttctgca tagttctggc gatacgtttt tatatactgt aattttgctc
      781 aaaatttggc gaatagtccg aggagaaatt tgctgatcat gtgaggaacg aaaaacaaaa
      841 gaattttcgt ccgtattttg tgctgttaac ttaaaatagt tctccagaaa tggcatcgct
      901 tcttttggta ttggtacttt attcagtctg ttttcttttc tattcagatt aagaatctgc
      961 ttggtcaaag atacatcttt acgtttaagt tctactactt catggatctt taatccgcaa
     1021 tacagaagaa tggcagtaat tgcaaggttc ctcggtctat tcttttcttc aaagctagct
     1081 tctctgcttg tttgaaagcc ttcaatcggg ataaacaaat gctcaaaatc tttgaaaagt
     1141 tctgcatcga tatatcgcgg agctgctttg gttgtttccg gtaaacttgt acccaaaaga
     1201 gaaatctctt cttcaatcag tcctttttga tacaaaaatc gtaaaaaatg atttaatgaa
     1261 tattgcttcc ttgaaatccc gtaatagcta ttttcaaaca attgctccgt ttcttggttc
     1321 gctgtagttt ggaacgaacg agtatgtcga tagattttag ctaagaaatt ctgataatct
     1381 tgtttttcta aatggataat cagctgatca tccacttctt cagaaaatac ttttctcaaa
     1441 taatcagtaa acagatgaaa atcttttgcg tattcatagc gactgcctaa tgaaagggaa
     1501 gaacgatttt cttctaaaaa ctgttgaaag acggaaggga gcaagtgtgt acgttgcttt
     1561 aacttttctt gcagttgttt tctagttttg ctgtccatta caaattccct ccaatagtgt
     1621 tcattttcta tattttaccg catttcatca aatgaagata gacagtttgt cttgatctta
     1681 ggaagcaaat ttttaactaa gtactagcta aaagatatga agtaagaaga tacattttta
     1741 gttattttat tagtgcagtc attcttttga tcttttaatt gatttatcct ctttttagct
     1801 atgtggttac ttcatgagca atgaaatgga tttggttcgt tctaatggca ggaggctctt
     1861 tcttttattt ccgaaaaatt agaaaacatt ttttcagaat cgattggctc tccatttatt
     1921 caataatggt ttgtctaacg atattgttgg gataattctt aaagaagtat tagaaatagt
     1981 tggtacttca ttactttaca tgaagattta ttctatggta ggagcaatta tattgagaat
     2041 taatcctttt ttatataagt tgtttgctta ttgatggatt ggataaatta aaccatcaca
     2101 acgagtggaa attatatgaa atttttcata aaaattcata tagaaacaaa tgacattt
//
LOCUS       DASJSZ010000179         2118 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.186, whole genome shotgun sequence.
ACCESSION   DASJSZ010000179 DASJSZ010000000
VERSION     DASJSZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.186"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            731..1063
                     /locus_tag="U4B03_002842"
     CDS             731..1063
                     /locus_tag="U4B03_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEN2069626.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1682..2023
                     /locus_tag="U4B03_002843"
     CDS             1682..2023
                     /locus_tag="U4B03_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEN2069627.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          745 a          279 c          438 g          656 t
ORIGIN      
        1 atagctattt taatagaatt tataagttaa aatattatga taaaaatcac aaacgtagcc
       61 ttttacgtca taagtgttaa aagaaataat taatttcaaa tatagaaaag ggaacgtatg
      121 tttgcatact cctttttttt gagttataat aatacgcgaa gagagataat ttcattattt
      181 aaacaagcta aaaatcttga tattacttct tttcaggcaa tacgaatatt attcgcttac
      241 attttttgta taaatttaat tgtctgctat gcttgtgatg aattgatttt cgttttgcac
      301 ttttggatga taaatttgga caattgaaaa tacaaaaaaa agaaaggcgg tatgaactcg
      361 gaaggctggt aaccttcgtc agcgttcata cacggcatta agatttggcc cttaatgcgt
      421 taacctttca gtgttacggt attacccgca atggatttag tataacagat attcaaaagt
      481 gataccactt tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg
      541 cctaagcata atgttataaa aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa
      601 ttagagggaa cgcatgtttg gatgcgttcc ctcttttttg tttgtcatga acgaaaatca
      661 attctctatt tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa gaaattgggt
      721 ggtttatttt atgtcatata caggaatttt gagtctcaaa gatatttgtc actacggtaa
      781 acggtgtacc gctacagaga aaatcacgaa gaaactatca actggccaaa gtaaaaccgt
      841 tgtgcagtgt aagaaatata taatccagaa agacaaagtc tcagaggaaa tgatttatta
      901 cattgggaaa caaaagcaaa taattttaaa agatcctatc cccttaaaag agctatatcc
      961 aacaatcaaa catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt
     1021 cttgcgttgt accgcaaaag ggatggggcg tttgatcggc tgaattgatc attctcataa
     1081 cttgtatttt atcgtttttc gcttgcttga aaatattgtc gagacttcta aaggaactta
     1141 gagtcactag aaaaataagg agtagacttg atctatcaac aattcccttc ggggagtgac
     1201 ggggtaggtt ttaacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcgcttgtt
     1261 tggatagtgg tgaaatgaat ttgtgtaaat aataagcgca gagtaaacaa gagggaattt
     1321 agaagttaga caagttagaa tagcgtctag attaatcatg agcagctact aagggatcta
     1381 gactttgacg caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca
     1441 agaaattgtg tcgtgtgctg cagtacatgt agatacttgc gaggacgttg attattggat
     1501 caatcgagca tttaccgctg tctactaggg tttctcgttt cggacgccat aaacgagtcg
     1561 aaatttttta tgtattttta agtaaacgaa aaacgataaa atatgaacaa gtcctgaata
     1621 aagtgaaaaa attgatataa aacatagaga aaatgaaatt tacgtaatgg aggaatgaca
     1681 aatgaaaaat tatgatccga atatacgatt gggcacacat actattaagg tttctttcca
     1741 acgttgggat tacaaaggat ttgtgacgtt tagaaggggc ggaaattgta aaggattaga
     1801 tgttttagca ttagacgagg atgatttata cgatcaaaca ttgactgata atccaattgg
     1861 attcggcttg ctacctgaag atgatgaagg taatgaatgg tttaaaatga cgcttatgaa
     1921 tgataatgga gacgaattat ctgttgaaga tacttggtca tatttgagtg attatattgt
     1981 cagctttgaa atcattgaat ttgtagcgga taaagaagaa taaaaactgg aggaataaat
     2041 tttacagata aaggcttaga aggagtcacg acgattaata aaaaggtagt attaccttag
     2101 gcattgagta aggaattt
//
LOCUS       DASJSZ010000180         2103 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.187, whole genome shotgun sequence.
ACCESSION   DASJSZ010000180 DASJSZ010000000
VERSION     DASJSZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.187"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(76..1002)
                     /gene="cysK"
                     /locus_tag="U4B03_002844"
     CDS             complement(76..1002)
                     /gene="cysK"
                     /locus_tag="U4B03_002844"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEN2069628.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1142..2029)
                     /locus_tag="U4B03_002845"
     CDS             complement(1142..2029)
                     /locus_tag="U4B03_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN2069629.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          581 a          471 c          356 g          695 t
ORIGIN      
        1 atcataaatt tttgtgtgtt cttttcatct ttaaaagttt tttacataaa cctatctgga
       61 gaaaaaccaa gtgccttata tctcttggta aagggctgta gataaataac gttcaccatt
      121 gtcgggaacc acagctaata ccttcttgcc ttttcctaat tcttttgcga cttttagcgc
      181 ggcagcaatt gcagcgccag atgagatgcc tactaaaatc ccttcttttc gcccaacttc
      241 tctagctgtt tccatcgctt cgtctccact aatagaaagg actttatcat acacagaagt
      301 atccagtgtt ttaggaacaa aacctgtacc gattccttgg attttgtgag gtcccgcttc
      361 tttcccttct aaaatagcag attctgctgg ttctaccccg attaattcga ttttcggata
      421 cacacgtttt aattctcggc cagcgccagt gatcgttcct cctgtaccga tacctgcaac
      481 aaacgcgtcc aagccattta ctccaaatgc ttgatgtatc tcaggaccag tcgtcttctc
      541 atgcacaaga ggatttgctt cattttcaaa ttgtaatggt aaaaagtagc cgttttcttt
      601 tgccaaacgt tctgcttctc tgattgatcc agaaatacca tccgcaccag gagtcaaaat
      661 caactctgcg ccgtaccctt tcatcaataa acgtctttcg atactcatcg tctcaggcat
      721 cacgatgatt actttatatc cttttgcaac tcctaccatc gacaatccga ttcctgtatt
      781 tccagaagta ggttcaatga tagtatcacc tggtttcagt agaccatcgt gttctgcttt
      841 ttctatcata cttaaagcaa tacgatcttt tacacttcct cctggattaa aaaactcaag
      901 ctttacaaaa acatctgctg aatcttctgg tacgatttta tttaatttga cgattggcgt
      961 tttgccaatc aattcggtga ctgagttata gatagctgtc atcttcaatt cctccttgtc
     1021 tttgctcttc ttacttatca taatacaatc tgttcattta tggcgagtga ttcgtctttg
     1081 actagcaaaa aaaagacaaa gatataccat atatggtaat ttctttgcct ttttatctaa
     1141 atcaaacgtt tagttcttga ccaactaata aagaataact ggacatagac atgccatttt
     1201 tcgccattag atcatcaaca gacatgccaa atttttgtgc aatatcccat agcgtatcac
     1261 ctgacacgac tgtatatgtc tgaccattat ttgtatttac atctgttgtt gaagatgatg
     1321 acgttgaagt cgtcgtagta gtcgttgttg ttgtacttgt ggttgttgta gccgaacctg
     1381 ttgatggcgt atcaaaacgg gttaaattat aagtattaat caatgagttc aatttggccc
     1441 cataatttgg atcggtagca taacgacctg tcaagtacgc tgttgcatca taaaaacttg
     1501 ttgtattgct tttccacacg ccagcataat gataatcgcc cgtggaaaaa ctggttgaac
     1561 gaagaacatt cgcatggtct tggaaggatt cccaataaga agaatatttt cggaatggct
     1621 cattcatcgt tacccattgt ccgtttacat attctttggt tgccatataa acgacctgtc
     1681 cgttatagct tcctttcaca ccgaatagat tataatttgg cgcactagct aatccggaag
     1741 tgccatatgc actttccaac aacgcttgag cgatcatgac tgaagcatac aagtcattac
     1801 ttgcagcaac ttctgacgca gaccagccga tttgatcgat aaacgcttga ggatcagcga
     1861 ctgtttgaga tccttgttca tctgcatgaa catgttgcgg taccgctgta accatcatcg
     1921 gcccaactaa aagactcgtt ccaattaaag gagcaatcgc ttttttattg atcaccattt
     1981 ttcttttagg tctatggtgc cgggcactgc gggtcttcag ctcttgcatt caaattcctc
     2041 ctcaaaataa aaaaacttaa taaaaagttc tcttcttaaa acaaacttaa ttaattaaat
     2101 tat
//
LOCUS       DASJSZ010000181         2079 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.188, whole genome shotgun sequence.
ACCESSION   DASJSZ010000181 DASJSZ010000000
VERSION     DASJSZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.188"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..647
                     /locus_tag="U4B03_002846"
     CDS             <1..647
                     /locus_tag="U4B03_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN2069630.1"
                     /translation="NGQWMDVVQPTTVDGFKACTDPRQAAYAFERNYERPAATHPERQ
                     DYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGA
                     GTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSW
                     FADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSIVIPPK"
     gene            660..1568
                     /locus_tag="U4B03_002847"
     CDS             660..1568
                     /locus_tag="U4B03_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN2069631.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1568..>2079
                     /locus_tag="U4B03_002848"
     CDS             1568..>2079
                     /locus_tag="U4B03_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069632.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          694 a          306 c          500 g          579 t
ORIGIN      
        1 caaatggaca atggatggac gtagtacaac ctacgacggt tgatggattt aaggcctgta
       61 ctgatccaag acaagcagca tatgcttttg aacgaaacta tgaacgtccg gcagcgacac
      121 atcctgaacg tcaagattat gcagttaact ggtataacaa atttaaagat ttaaaaccag
      181 gaggagctac tggagaagca ggactaaaac atttagagtc tttgattgga caacgaattg
      241 gcaatggtca gtgttatggc ttgtctgcag aatattcagg atatctgggt ggttgtggca
      301 tgggtgctgg aacaaaatat ggtttaactc atgtgatagg aaatacttct gcagcatctg
      361 atattggtat tgcctatgat tggtctgctg ttggttggaa agtgattcaa aaccctagat
      421 atgatcaatt agtagttggt gcaattatta attgggcaag aggtggacaa gtgggttcat
      481 ggtttgcaga tggaacttat ggacatactg gcgttatccg aggcttagct aatggtcgta
      541 tgcaaactta tgaacaaaac acagagttag gtatgatttg tggaaagtta gatcgccagt
      601 attatagtgc aagtgcaatt tcttccattg tcataccacc aaaataggga ggtgatttaa
      661 tggcaaagtg gaatgtcata ttaagtacaa cagaacccta taattacgtg gggatgattc
      721 aagttcgaca aggcaataag aatacagagg ttatggaagc gactatagtt gaaaatggtc
      781 ttccctacga tttatcagaa tgtaaggtat attttgaatc agttgtaggt gggaaatatc
      841 cagtccaatt agaaacaaaa attgtggatg ctaaaaaagg gaaaattaac tatatttttg
      901 ataaatattc catgcagtgt ttacatcgac aaacagccaa tttcattata tttaaaggag
      961 aagacttgat tggaacaact caagacttct cttattttgt cattaatgct gtttcaaaaa
     1021 cagaaggaga aatgggttct tattggcaat caatcgaaga tttaattgcg gatatgacgg
     1081 actttattaa tgaaaataaa ggcgatttta cggactggat gaatgaaagg aaagaagaat
     1141 ttgaacgttg gagacaagaa caggaacaca gtttccaaga ttggagagaa ggacaagaat
     1201 cagactattt ggcatggttt gaatcaatca aggatatttt aaagacgatt gatccaggtg
     1261 gagtcatgtt agccgaacta acggatgcgc gagtagatat tcaaggagtt cgtcatgcgt
     1321 ctatttcaga gcgtttgctg gcagatatgg actatttata tcagaaattg cgagcaacgc
     1381 ttttcacgat tgaatacggg gagattgaag tgactgatat tttgcaggat gatctctttt
     1441 cagataatca tgaagtcgaa aaagttggaa ctgtagcatt cccgattgaa gaaggagcct
     1501 tgatcatcgc aaccgttgat gatccaaagc aaaatgtgtt cactcttgag aaagttgggg
     1561 tgatctaatg gctaaaacta aacgaatgat ggaaacggat gaaaaaacgg gtgtacaacg
     1621 ccaattcttt ccaatcacac atgcttccgc agttcttggg ttagaagaaa taatagcagg
     1681 agaagcaaca gttttatctg ttaatggaaa aattggagcc gtcgtcatta ctaaagaaga
     1741 tttgggatta gagaatgttc tcacagaatt accctatgca agtgaagaag atgacggcat
     1801 tatcacggca gaaatgtatc aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc
     1861 agttgccact atcgaccgtt tgggtggcat aaaagttggt gaattattga cgattgatga
     1921 gacagggaaa gtctctgcag tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga
     1981 aaaactcgat tcactgaaaa attatactgc tggagaaaac atcactattg atgaagatgg
     2041 aacaattagt tctacaggtg gtagtgggac tggtggtgt
//
LOCUS       DASJSZ010000182         2071 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.189, whole genome shotgun sequence.
ACCESSION   DASJSZ010000182 DASJSZ010000000
VERSION     DASJSZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.189"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(119..709)
                     /locus_tag="U4B03_002849"
     CDS             complement(119..709)
                     /locus_tag="U4B03_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEN2069633.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1232..1393
                     /locus_tag="U4B03_002850"
     CDS             1232..1393
                     /locus_tag="U4B03_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2069634.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1412..1906
                     /locus_tag="U4B03_002851"
     CDS             1412..1906
                     /locus_tag="U4B03_002851"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2069635.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            1899..2060
                     /locus_tag="U4B03_002852"
     CDS             1899..2060
                     /locus_tag="U4B03_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069636.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          744 a          328 c          356 g          643 t
ORIGIN      
        1 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
       61 ccatcagtaa aattacttgt catactattg ataaggagta tttaattgaa gagaatgatt
      121 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
      181 tctttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
      241 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
      301 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
      361 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
      421 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
      481 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
      541 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
      601 tagaagcagt tttgttttgc catccctctt tttggtttag tgtgctagta atataagcac
      661 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
      721 cctttactat ctatgacaag gacaacagtt ttaacataca gacattcatt aaatttggat
      781 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagaca gtcaacttaa
      841 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
      901 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tcttaaaaga
      961 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
     1021 tgaaaacaac cctagaaata tataactagt ccaatgaaaa aaaagcgaat gccgtagaaa
     1081 acaaggattc tgcgtacatt catatctcat tttgcgagca tatctgctat tactgtgact
     1141 aaatgttaat atggttaaaa tgaccgttat attttttata taaatatcgt ataattagta
     1201 tgaatataga agaaggaaag aaggtgcaat cttggaacct aaagacaagt atttagatta
     1261 cgctagggtc aataaagata aatttattga aacaatcatc aagggaaaaa tccctgatga
     1321 tgaaaaagat gcggtattca tggctggaag cccaggagca ggtaaaacag aggttgcact
     1381 aggtttagct taaaattatg acaaccatgt tgtgattgat gcggactatt ttagagcaca
     1441 atttccagaa tacaatggaa agaactcaag tgtttttcag aaagcttctt cttggttagt
     1501 tgaacaatca ttaaaatatg tactggaaca tggctattct ttcatactag aggatacttt
     1561 tgctatcctt agtgcagaga aaaatattat tcgagccctt aaaaataact ttcgcgttac
     1621 gattttctat gtttaccaag atcccaaagt tgcctgggat ttcactcgaa aacgtgaact
     1681 agctgaagga cgtcatgtgc caaaagaaat gtttatcaat gcgtttttta agtccagaga
     1741 gaatattgaa aaagtcaaaa atagacatcc tgaagtagtc cttcacataa tgattaagga
     1801 ttatcaaaac gacatttctg aagtgcatta tgcagcagat aatattcaat tagtactacc
     1861 aatacaatat acttcaaaag agttggagga ggaattacat gactgagaaa gaacaagtac
     1921 aacaaatcgt gaagaagtat aataaaagta tcgctgattt atcagaaaac gcctcagcga
     1981 aagaatttaa aacagtaatg aattacgtag cagatgaagc aaatcgtaag cagcgaaaac
     2041 tagttgggtt ggataaataa attttttaaa c
//
LOCUS       DASJSZ010000183         2048 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.190, whole genome shotgun sequence.
ACCESSION   DASJSZ010000183 DASJSZ010000000
VERSION     DASJSZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.190"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..214
                     /locus_tag="U4B03_002853"
     CDS             <1..214
                     /locus_tag="U4B03_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2069637.1"
                     /translation="DKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFPFL
                     YPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            211..>2048
                     /locus_tag="U4B03_002854"
     CDS             211..>2048
                     /locus_tag="U4B03_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2069638.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          764 a          247 c          407 g          630 t
ORIGIN      
        1 ggataaatat gatttgaaat cgttggacag agaattattt atagattcta aattagaaga
       61 ggcttatcga aaattagatg gtaatttaga acatcaggat caggtcacct tatttttaga
      121 ttttccattt ttatatcctg gaaaaaatga aattaaatgg acgaaaaata ttcattcatt
      181 tgaaataatg cctaggtggt ggagaaaagt atgaaaccaa gaatatatag tcctactgaa
      241 acagatttct caacgaatgg cttaggaatt ttaaaggatt gtacaagatg tgaaatatat
      301 gaagtagcta atggaaaata tgaattagag ttggattatc ctttaggaac tagatttgat
      361 gaatattttg aaaatgacta tcaaataaaa gcaaagccaa acgatcaaga agagtatcat
      421 atctttttta ttgatgataa agatatagat acttttttaa atacagtaac tatttatgct
      481 cagagtcgta caaatcgact tggaagacgg gtagtcactc ttgtggaagt ggactctaaa
      541 actggtcaag aagcgatatc aattattgaa actaaaatgg ataaaaaatc tgacatacga
      601 ctttattctg atattacggc tgtttctagc acaatctttg aagcgagaaa tgttttaaat
      661 tgtattgctg gtgaacaagg atcattgctt caatattggg gcggagaaat taaacgtgaa
      721 ccatttaagc tttctttgtt aaagcgaaga ggtcgcaata atgttggaac gattcggtat
      781 ggaaaagata tgtctggttt gaaggtcaaa ttagattgga caggtataaa aacaagaatt
      841 attccatatg ctgatcctca aagtgaagta ggtacgacta atcgaattta tggttcgcca
      901 gtagatagtg cctatattaa taactatcct gatgtctata cagaacatgt tcagtttaca
      961 gaagaacaag gagtaaaaga cattaagagc ttaaataaaa tagctaaaaa ttactttaaa
     1021 acgatcaatc ctggctgtga taagcctaaa gtttctatta cggttgaatt tgataagttg
     1081 actgatagtg aagaagcgaa agaatttgcg aagattagaa attatggtct gtttgataca
     1141 tttaaaatat atcataaaaa atataatatt tatcttgaat caaaggttag tggactacaa
     1201 tacgattctt tatctgaaaa aactttgaaa ttagaagcag gagacattca agttgctttt
     1261 tatcagcaac aagctgtaac tattcaggat aaattaaaag attatgcaac gaataattat
     1321 atgagtgatt ttaatgatta tgtttcttca atgattacag gtcaaggcag tgcaggagga
     1381 tatgtagttt tatggccaaa agaaaaacct tccaacatct ttattatgga taatccagat
     1441 ttagaaaagg caagagaagt actaagaatg aataaaaatg ggattgcttt ttctaagaaa
     1501 ggatggcggg ggccttttaa ttcagcttgg acattagata gtatatttaa tgccaatttt
     1561 atacaaactg gattaatcaa agcagatatc tttcaaaatt cttttaacaa gactggagat
     1621 gtattgaaat tagttaatgg actactccaa atttggaaca acaaaaagaa aataatggag
     1681 ttaactaaaa aaggaatgga attttggaat tctaagggtt caattggaac gattggaaca
     1741 actgattctg ttggtaatcc ttttcctggg gcttctactc caacccctat tgaagataat
     1801 tctttagtta ttcgtacaaa tggagacggc aaatatattt tgatttctcc taaagtagga
     1861 aaaggattag ttttattagg aaatggtaaa gcaatttatt ttggagactt agatgtacaa
     1921 ggaaaactta cagttaaagg aaaagaaata attcctggac aaggtggtgg tccatcggga
     1981 ggtggagaat ctactggtgg gtatcctagt gaattaaaaa cagatgcaga aaagagagct
     2041 tggagaat
//
LOCUS       DASJSZ010000184         1970 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.191, whole genome shotgun sequence.
ACCESSION   DASJSZ010000184 DASJSZ010000000
VERSION     DASJSZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.191"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..721
                     /locus_tag="U4B03_002855"
     CDS             <1..721
                     /locus_tag="U4B03_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2069639.1"
                     /translation="NALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            739..1308
                     /locus_tag="U4B03_002856"
     CDS             739..1308
                     /locus_tag="U4B03_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEN2069640.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            1418..>1970
                     /locus_tag="U4B03_002857"
     CDS             1418..>1970
                     /locus_tag="U4B03_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2069641.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQ"
BASE COUNT          667 a          247 c          377 g          679 t
ORIGIN      
        1 taacgcatta aggaaatttg acatttttaa tagttcgatt gaagttccct atatcgaaaa
       61 ggtaagtgca gatgtattaa aagttgaaga aattacttat gaaaatattc taaaagacat
      121 aaattttgat gtcaaagttg gagatgttat tggtttggta gggaaaaacg gtgtggggaa
      181 aacaacctta ctaagaatac tcatgaagat tattcgccct accaaaggaa agataatcga
      241 aaataaaaaa aatttgtcat cgccgttttt agtaatgcaa gaaatggatt accaattttt
      301 cacagaatca gtgaggagcg agttagccct ggggaatgaa atagttagta aagatcagca
      361 agaaaaaatt ttaaaaaaaa tggaacttta taaaatgaaa gatcaattac cgtttgattt
      421 atccggtgga gaaaaacaaa gattattggt ttctatagct tcattgtcaa aaactaattt
      481 atttctattt gatgaaccta caagtgggtt ggattatatt aatatggaaa ggattgctga
      541 ccttattaaa gacttaaaag aaaagggtgc agtagttata gctactcatg atccggaatt
      601 tctttataaa acatgcaata gaattattta tttaaaaaat ggtggaataa aaaaagatat
      661 aaaacttaga gtagaagatt caactttgat agaacagatt tttaatgaca taacgaaata
      721 gaattgagga gaatatatat gaaaacaaaa gattatattt ttattggttt agctacagtt
      781 atttccctag taatttactt tttttcaata atggttagta gtattggtgg ggcgtttggt
      841 cacagcatat cccccgggat ttttggatta ttatctggaa tcatatttgt ttatattagc
      901 tataactacc ctagaaaagg aatttttact atttacacaa ttgttttatt gttatttttt
      961 acgttaatgg gcggggcgta cttaccttgg tttatcagtt cgattagtat ggcgatctta
     1021 gctgatatta ttctatctgt atttggatat gatcgagcaa ttcctcaagt tgcgtcttgg
     1081 gctctaatgc aattaggcag tgctgccgga cagtggattc ctatatggtt ctttacagat
     1141 cgttttcgtc aagattggat agataaagga caatctgctg caacaatgga tgcaatgatc
     1201 cactatgcgg taggtatatg gggaatcatt tcagtgttag ttgtagcaag tttatctatg
     1261 attggtgtat tgattggaag aaaagtctta aaaaaataca aaaaatagaa cgtagtttac
     1321 cttttatctg tttgtacatc tctaatttac gctattacgg aaagcattaa cagttgtaaa
     1381 cgaataagag aagaaattga atgcggggga ggaaaaaatg aaagtataca aaaaattatt
     1441 tagttatgtc cctaaaatga aatactacgg atggttagcc actttatttt ctgatttttc
     1501 tgtcgtatta acagtctatg gatattacag tatttacaaa tttcttcagt ctctaattat
     1561 tgtaggagat gaatatttag ctaagagtta tgcggttcaa actgttgctt ggttaacttt
     1621 aggagcttta ttttatattt tttctggctt attttctcat atccttgggt ttcgcttaga
     1681 gactaatttg agaaaaaagg gaattagcgg tttaacagaa gcaagttttc gtttttttga
     1741 tttaaattct tcgggatata ttcgaaagac aattgatgac aacgcggcta aaacacatat
     1801 ggcaattgct cacttgattc cagatagtgc tcaagccata gtaacacctg ttttatcagt
     1861 tgccttaggc tttttcatta actttaaaat tggattggtt ctaatcgcca tgttagtaat
     1921 tggtgtattc ttgttaaaga aaatgacaga gaatacaaat tttattcaaa
//
LOCUS       DASJSZ010000185         1892 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.192, whole genome shotgun sequence.
ACCESSION   DASJSZ010000185 DASJSZ010000000
VERSION     DASJSZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.192"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..101
                     /locus_tag="U4B03_002858"
     CDS             <1..101
                     /locus_tag="U4B03_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN2069642.1"
                     /translation="ELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            102..854
                     /locus_tag="U4B03_002859"
     CDS             102..854
                     /locus_tag="U4B03_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN2069643.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            859..1806
                     /locus_tag="U4B03_002860"
     CDS             859..1806
                     /locus_tag="U4B03_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN2069644.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          656 a          258 c          378 g          600 t
ORIGIN      
        1 aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg aatgatttat
       61 atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt ttatttacta
      121 tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat ttggcaggaa
      181 agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa catcctgaac
      241 ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg acgactaagt
      301 taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat actgtaggga
      361 aaattgaagt gatacgtgat tcattcttac aactagaaag agaagataat atatttgatt
      421 taattataga tttagatttg acttcacctt tacgtgaagt ttctaatctt gaagaattaa
      481 tagatgaaca tctaaaaaat ttagataaag acgtctactt ttcagttgtt tctgcaagac
      541 gtaatcccta ttttaatatg gtaaaaagaa ttgaaaatga agttactata gttaataaat
      601 caagatacac atctaggcaa cagacacctg aagtttttga aatgaatgcc tcaatgtatt
      661 cttttaaacc tgaatttcta aaaaaaagta attatatttt tgatgggcaa tgtgggataa
      721 taaaaatgag agattatcta attttagata ttgatagtga agaagatttt aaatggttag
      781 aatttttgtt tcctaaattt ttagaagatt cttcggagct tcaagatatc tataattatt
      841 tatatcggag gtaaaaggat gagctgttat cttatcaccg gtggggctgg cttcattggt
      901 tctaccttag ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga tgacttatca
      961 atgggaaaag tagaaaatct tgatatgacg aaaaatatca cttttattga gggagatgtc
     1021 gctgagaaat ccttgatgga agagatcatg aaagctaata aatttgatta tatctttcat
     1081 ttagcagcag ttgctagtgt ggcagattca gttgcccgac cagtagaaac acatcgtgtg
     1141 aattttgaaa gtgtattaat gattttagaa ttgatccgca agtatcaacc tgatttaaaa
     1201 cggatcgtgt ttagttcttc agcggctgtt tatggggatg aaccgacttt accaaaaaaa
     1261 gaagagtccg tgattcgccc attgacgcct tatgcggttg ataaattcgc tgcagaacaa
     1321 tatgtgctag attattgtca tttatatgat gtacccggta gcgcagttcg ttttttcaat
     1381 gtctatggtc caaatcaaaa tccgaattct ccatacagtg gtgtgatttc tattctcgta
     1441 gatcgctata agaaacaaat cgtaggagaa gcaacttcat ttacattatt tggtgatgga
     1501 agccagtcta gagactttgt ttatatagat gatgtaatcc aagcattact tttagttgcg
     1561 aatgaggaag ctgcactggg gaaacaattc aatgttggaa cgggtaaagc aaccacactt
     1621 ttggaattga ttcatgcaat caatgaaatt ctcggtgtag aattgacact tgaatacaaa
     1681 gaagagcgtt cgggtgatat ccatgactct ctagcagata ttactaagat ccagtcaatt
     1741 ggctatcaac cgaaatatgg tattttaagt ggaatgacta attatttgaa aactgaaatc
     1801 aattaaaaca tactaagata agattagagg gtaaattcag gctctttgtc aataaggact
     1861 gatgagtttt gtaaaatcaa atctgggata ga
//
LOCUS       DASJSZ010000186         1888 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.193, whole genome shotgun sequence.
ACCESSION   DASJSZ010000186 DASJSZ010000000
VERSION     DASJSZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.193"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..325)
                     /locus_tag="U4B03_002861"
     CDS             complement(<1..325)
                     /locus_tag="U4B03_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2069645.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLI"
     gene            complement(322..975)
                     /locus_tag="U4B03_002862"
     CDS             complement(322..975)
                     /locus_tag="U4B03_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2069646.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1263..>1888
                     /locus_tag="U4B03_002863"
     CDS             1263..>1888
                     /locus_tag="U4B03_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2069647.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          599 a          337 c          244 g          708 t
ORIGIN      
        1 ttatcaaaga attactatca ctttctaaat taacatttat attatctttg atttgtattt
       61 cattcttatt atttttttct tttatgaata cttttatttc actactcttg gaataaaact
      121 ccaaagtttg ttctatatat tttatttctt ttccattcat attactagaa atttcatttg
      181 ccatattatg aatcttttct tttctcgtct ccaaatatgt tttaggaaag ataaaaaaaa
      241 ttaatgaatg aactaatata attattattc caagaacaga aaatatttgt atgaacattt
      301 ttggaaatat ctttaatttt ttcattttct ctccaattta tatcctacat ttcttatagt
      361 agttatacag tctaattgta atttttttct aagttctttt atatatacat ctattactcg
      421 atcataagga gtttcttcgc tatctttcca tacataatca ataatttgca ttcttgttaa
      481 cacttgtcca ttattatcca ataaacattt taatacttct agttcttttg catttacatc
      541 tatttcttca ccatttattt tagctgtata gctattaaaa ttaaccgata gatccttata
      601 ttcaaacttc tctaaatgtc cataattctt cttaattaaa gaatctattc tagcttttaa
      661 gaccggcaat gaaaatggct tttctacata tccgtctact aaattagtaa atgcatcaat
      721 tttatattct tcatcactaa atgcagtcaa gattagaatt ggtaaattgc ttttctttct
      781 aatttctttc aacacttcta aaccatttat aaaaggtatc tgaatgtcta agataaccaa
      841 atttatatcg ctattaaatt ttgacaaggc ttctcttcca tctttagctt gaataacagt
      901 atatccaaat tctgaaagat attcacttat tccctctctt atcatattat catcttcaac
      961 agttaatatt ttcatatata cctccactta agcctataca ctagatatta tacagcatat
     1021 tatggctcta taatatctag tgttggtgac accttctgtt gccaatactt tttctttttt
     1081 ctaataaatg caaacagaca ccttgaaatc ctttagaata aagggttcta taatattttg
     1141 tgttgttgga aaatcgaagg cgattttcta ctaacacttt ttcttcgttt caactcaaaa
     1201 agagactcac ttttggtaaa atttagtcga ccaaaacatc accctaccaa aggagtcgct
     1261 ctatggataa tcatactaga aaattactcg tattaacaga taaacattta ttttttgatg
     1321 aagaatggct gattgaaaaa gaatataaag gcgttcaagc gcagtttatt aaagggagat
     1381 tggatgactc accttcccat tgtgaacact gtggatcgat tcgtattatc cgcaacggtt
     1441 cctatacgac acgtactcag atattgaaag tcaaagaaaa gttgactatt ttagaattaa
     1501 aacgtacccg ttttctatgc tatgattgtg ggcagacctt ttctgcgaaa acggacttag
     1561 ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc gattctcata gaactgtacg
     1621 aaaatcaatc acgtaaacta atcgctaaga agtacttcgt ctcggatggg acggtgacac
     1681 ggatcttgcg tgaagcaacg aagcattacc agccacgtat gaacttcctt ccgacggtgt
     1741 tgtgtatgga tgagttcaaa tcaatgaagt ctgtttctgg ttccatgagt tttatttgtg
     1801 ttgatgggac aacccatcag ctatttacga tccttgaaga tcgcagactt tacaaactaa
     1861 ctcaatactt cctgcgcttt cctagaaa
//
LOCUS       DASJSZ010000187         1825 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.194, whole genome shotgun sequence.
ACCESSION   DASJSZ010000187 DASJSZ010000000
VERSION     DASJSZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.194"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..462
                     /locus_tag="U4B03_002864"
     CDS             <1..462
                     /locus_tag="U4B03_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069648.1"
                     /translation="KCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRAR
                     TNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLI
                     LNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            449..>1825
                     /locus_tag="U4B03_002865"
     CDS             449..>1825
                     /locus_tag="U4B03_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN2069649.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIY"
BASE COUNT          595 a          235 c          276 g          719 t
ORIGIN      
        1 aaatgtatct tttttgatca acctttatta tctgatggtt atggcataga tgaaacagat
       61 attttcgcta aagtaaatag tatattttct ggaaaaaaag aaactttaat taagcttcat
      121 cctagagcaa ggacaaatcg ttatggtgaa aaaaacatat tgaattccag tttaccgttt
      181 gaagtcttgc tacttgaaaa tgacttctct tcaacacttt tgattagtcc tatatctaca
      241 atttcttttt caccatctat gatgtttgaa aagacgatca actctattat gttggctaaa
      301 ttaattttaa atgaatatac agcattatct gaagataaaa aagaaacatt gacaaatatt
      361 gttcttttct gtgaacatta taaccgtcta tcagaagaaa aaattttact cccagaaaac
      421 tgggcagaat taagaaagtt ggtagagtat gggattattt aacacacttt ccaagtcaaa
      481 attagttaag tccagtttct ggtatactat agggaatttt tttattaagg gaatttcatt
      541 cataactatt cctatttttg taaatattac tactcaagca gaatatggtt tgattaataa
      601 tttcacttca tttgtatcaa ttttttcgat tcttgttggg ctaagcctaa attcggcaat
      661 taataatgca agttttgact ataaagatga cataaatgaa tttatgtcat cggtactttc
      721 tctagctaca ctttcgttaa ttgtgttctt attaataggg aacatatatt ttttgttttc
      781 aaatgctttt tttgatatga accaacttgt atttaatttg atgttattac aaagttttgc
      841 tagttttgta attagttata tccagtctta ttttacgatt aactttaacc attttaaata
      901 tttgatttta tcttttatat ctactttttt gaatattgca atctctgtag ctttgatgca
      961 gacattatat tttgatgata aatatgttgg cagagtagtc ggcggaacac tgtcatttgt
     1021 gttgataggt ttagtaattt atttaagaat aatgataaag ggtgctcatt tttttgataa
     1081 aaaacagtgg agatacgctt taaaaatctc tttgccgcta atcccacatt ccttagcaaa
     1141 tatagtttta gcacaattcg atagaattat gattaattct tatttaggtc ctgctgcggg
     1201 gggaatatat tcctacatta gtaacatagg tattattcta agcgtaatat ggatgtctac
     1261 taataacgcc tgggttccat ggttctatgg tgagatgagt aaaaataatg aaaaaaaagt
     1321 gaagaaagtt agtaactatt atttagtagt ctttactttg attacgatga ttgtaatggt
     1381 agtttcgatt gatttagcaa aatttatggc tccgaaagac tattattctg ggttatcttt
     1441 agtggttccg ataaccttag gctatttctt tcagttttta tatagtttac cagttaatgc
     1501 tgaattttat gagaaaaaaa catcgtttat tgcgattggt acagtagcaa gtgctatagt
     1561 taatatacta ttaaacatga ttttcatacc taggttagga ataattgctg ctggatacac
     1621 aaccgtattt acgtattttt tactttttat ttttcattat aatatcgcta aaaaaattac
     1681 gaataaacaa ttatttgaca caaaaaaaat atgtatcata ataatttttg tatcaatttt
     1741 ttctttgata cttcatattt taatcaatca aatgttgatt cgttacgttt tgacatcatt
     1801 attgataatt agtttagtta tatat
//
LOCUS       DASJSZ010000188         1802 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.195, whole genome shotgun sequence.
ACCESSION   DASJSZ010000188 DASJSZ010000000
VERSION     DASJSZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.195"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            290..697
                     /locus_tag="U4B03_002866"
     CDS             290..697
                     /locus_tag="U4B03_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069650.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            711..1112
                     /locus_tag="U4B03_002867"
     CDS             711..1112
                     /locus_tag="U4B03_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069651.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1114..1485
                     /locus_tag="U4B03_002868"
     CDS             1114..1485
                     /locus_tag="U4B03_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069652.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1521..>1802)
                     /locus_tag="U4B03_002869"
     CDS             complement(1521..>1802)
                     /locus_tag="U4B03_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069653.1"
                     /translation="QLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNKNY
                     FKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          672 a          234 c          306 g          590 t
ORIGIN      
        1 ttttgagggg aagtacatat agttgttgat atagttgcta atattgttag ctaatttgtt
       61 agtaaaactg tatacgaatt tagatagtaa aaaaacagtt tgttccttat atagaggcac
      121 ttttactact gattaaatgt cagatatgct tacaaatata cttataaata tgcttacaaa
      181 attagctaat atttccgttg acaaagcttt gttactattg ttaaactaaa ttgactagca
      241 aaagtgaaac ataaatttat taaataaatg taaaaattag gagaaatcaa tgtcttatag
      301 agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa
      361 taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt
      421 ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat
      481 tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt
      541 gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga
      601 agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt
      661 tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata
      721 tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt
      781 gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta
      841 aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg
      901 atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa
      961 aatatgatga agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt
     1021 cctcagatta cgatgttttg gatactctcg ataatgtatt atctgactat aaatatatgg
     1081 ggaaatctgt agtagcaact ttgggggaat aaaatgtttg actttaatga atatttatct
     1141 atttccaaac aaattgaatc agatgataaa tataattcta aagaatctta tagaagaact
     1201 gctgtttcaa gagcatacta cagtgctttt aaaaaatctg atgagtattt gaaagaaaat
     1261 tatgatatta tttataatgg aagtagcgga aaaggaagcc accaaaccgt ttggaatttg
     1321 ttttcaacag ttaaggaatt gaatacctta ggaatacata atagtgggta tagaatgtta
     1381 gagaaaagaa aatgtgctga ctatattcct aatgaaacca ttacgaaaac agatatggcg
     1441 ctgatgaatc gagaagcaga aaaaataata aacaaactca cctaatgaat ttcaagcgag
     1501 tttgtttgtt atttttttat ttaatctttg tatctttctg gatcaacgaa agtatacttt
     1561 atatagtcat aacgccgatg atcgctacgt gcgtccggca cgtcagtcac gatatcaaac
     1621 aaaaagtata cacccttctt cattctagtt ttcgcagcag gaattttgaa atagttctta
     1681 ttagaatagt agagattgat taataagcta tcttcgattg ctaaaaagaa aacttctgaa
     1741 ttccatactt tataaaaatc tttgataaat ctattcgaag ggtcaaattt aaaccataat
     1801 tg
//
LOCUS       DASJSZ010000189         1760 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.196, whole genome shotgun sequence.
ACCESSION   DASJSZ010000189 DASJSZ010000000
VERSION     DASJSZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.196"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            278..820
                     /locus_tag="U4B03_002870"
     CDS             278..820
                     /locus_tag="U4B03_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069654.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="U4B03_002871"
     CDS             976..1425
                     /locus_tag="U4B03_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEN2069655.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>1760)
                     /locus_tag="U4B03_002872"
     CDS             complement(1455..>1760)
                     /locus_tag="U4B03_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2069656.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          655 a          256 c          334 g          515 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt
//
LOCUS       DASJSZ010000190         1759 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.197, whole genome shotgun sequence.
ACCESSION   DASJSZ010000190 DASJSZ010000000
VERSION     DASJSZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.197"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..471)
                     /locus_tag="U4B03_002873"
     CDS             complement(<1..471)
                     /locus_tag="U4B03_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069657.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNST"
     gene            576..821
                     /locus_tag="U4B03_002874"
     CDS             576..821
                     /locus_tag="U4B03_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2069658.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            977..>1759
                     /locus_tag="U4B03_002875"
     CDS             977..>1759
                     /locus_tag="U4B03_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEN2069659.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDA"
BASE COUNT          623 a          252 c          303 g          581 t
ORIGIN      
        1 agttgagttc gtgatttcat cttgggtctc attaaaaaag ttatccgaat aatcgcttat
       61 ttttgaaaag tctttattgg caccagcctc aaaaaattgt tcaacttttt cagtataaag
      121 ttgtgctaac gctccttttt caatattaac tttaatattt tcatgtctat caatttgtgt
      181 gctgttcagt aagggaagat cagcttttag ttttatatcg tcttgatttc tttcaatttc
      241 aattggcaaa atcttttcag tagattcagt ttctgataaa ggaacatcta gttttttatc
      301 atgataataa gcttcaaatc catctggaac cggaaaaaaa tgtattgggt aattcttttc
      361 tgaggttgtt gtactagttt ccgtacttgt agaacttgtg tcagaagtgg tacacccgct
      421 tatagttccg atgagtaaag cgcaagttag aatgattgca aatttcttca aaaaaattcc
      481 tccttaaaat tgaatatgga ctaatactag tcccattgta gcataccgaa tctttaagat
      541 taataagaaa aaggactaat tttagtccaa aaggtgtgct attcttgaaa gaggaaaaaa
      601 ttattgataa agaaaatgta atcggtagca atattagacg agtccgatta gaaaaaggaa
      661 ttggtcaaac cgagctgatt agagacctac agcttcgtaa aattgctata actagggaaa
      721 cgttggttaa gattgaaggt ggccgccaac acattaagtt ggatcaattg aaagccatca
      781 aagatattct acaggtgtct tatgaagatt tgcttcaatg actacttgta gtcatttcgt
      841 ttgaaattat tatgttacac ccttatgtgt cacgaagaaa tactggtata ataatgaatg
      901 ggatatgtaa cgatagggta tactgttatg ttgcctaata aattgtggat caaaatacaa
      961 gaatggaggg atatccttgt ataaggcata tgaattggat tttacaaatg ctgacttaaa
     1021 tgcattctcc tcaagctcgc aatcatatac acatgtagta aatcaaatag atcacaacca
     1081 aaaatctatt aacaagagaa tagaaaatct atttgaaagt gaaaatgatt tagttgatta
     1141 ttattctaag gacagcaaaa ttctagatgc agacgaaatt attaaagatt ggtttccaac
     1201 tataaaagca gatattttta tatctcactc tcatgctgat gaaaaattag ctatacgttt
     1261 tgcatcatgg ctatttgaaa attttggtct tactgcattt attgattctt cggtttgggg
     1321 atacagttct gatttattaa aaaaaataga ccaaaaatat tgctataagg aacaaacaaa
     1381 aacatatgat tatgataaaa gaaatgtaac aactagtcat gttcatatga tgctttctac
     1441 agctttaaat aatatgattg atagtacaga atgtctgttt tttcttaata cacctaattc
     1501 aattagtttg agcaatgaaa tcactaatga acagaaattt acgtattcgc catggctata
     1561 tagcgaactg acgacagctt caatagtgga aaaaaagaat ccacgattgg aaagtaatcc
     1621 tcaaatgagc accgaggatg ttagaagcat tataaagcac tattcttcgc aagaaaatct
     1681 gcaaataaaa tattctttaa atttaagtga attaacttat ttacgaaaag ctgatttgaa
     1741 aaaatgggct ttagatgct
//
LOCUS       DASJSZ010000191         1751 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.198, whole genome shotgun sequence.
ACCESSION   DASJSZ010000191 DASJSZ010000000
VERSION     DASJSZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.198"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(140..1288)
                     /locus_tag="U4B03_002876"
     CDS             complement(140..1288)
                     /locus_tag="U4B03_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2069660.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="U4B03_002877"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="U4B03_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEN2069661.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          517 a          302 c          278 g          654 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg g
//
LOCUS       DASJSZ010000192         1734 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.199, whole genome shotgun sequence.
ACCESSION   DASJSZ010000192 DASJSZ010000000
VERSION     DASJSZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.199"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(60..278)
                     /locus_tag="U4B03_002878"
                     /pseudo
     CDS             complement(60..278)
                     /locus_tag="U4B03_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(544..738)
                     /locus_tag="U4B03_002879"
     CDS             complement(544..738)
                     /locus_tag="U4B03_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069662.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(838..924)
                     /locus_tag="U4B03_002880"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="U4B03_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          610 a          253 c          229 g          642 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atat
//
LOCUS       DASJSZ010000193         1682 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.200, whole genome shotgun sequence.
ACCESSION   DASJSZ010000193 DASJSZ010000000
VERSION     DASJSZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.200"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1446
                     /locus_tag="U4B03_002881"
     CDS             <1..1446
                     /locus_tag="U4B03_002881"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEN2069663.1"
                     /translation="ADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIS
                     PEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRN
                     VTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQI
                     VTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLIL
                     HGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGK
                     LAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIG
                     MPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYA
                     ERKDVEKYAHLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVL
                     EKELLEAISSLQTTPENEAWLQGALEVFKHE"
     gene            1439..>1682
                     /locus_tag="U4B03_002882"
     CDS             1439..>1682
                     /locus_tag="U4B03_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN2069664.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
BASE COUNT          589 a          281 c          357 g          455 t
ORIGIN      
        1 gcagatgaat ccttagagga atatgatacg gtaagcaaac aaacgatgtt gtatcgcgaa
       61 ttactttcag atgaagaatc aaaagaagat ctgattgcaa caatcgttga tattttgggg
      121 tatgcgattt cgccagagta tcttttcaac gtcttagcgg atcaagcaaa acaagcaacg
      181 ttccaattga atgatctaaa caaagccttt gtgcaattgg catctaccta caatcaattt
      241 aatgggttat ttgatgatgt ggatctccaa tcaaaaaaac taggaacaga tgagcaacaa
      301 cgaaatgtga cgattaccga agtcattaag aaattgaatg acgtggaagt tttaggtcac
      361 gatggggacg tgatcgggga tgcttatgaa tttctgatca gtcaatttgc ttcagaagca
      421 gggaagaaag ccggagaatt ttacacgcct catatggttt ctgatatgat ggcacaaatc
      481 gtgacgttgg atcaaaaaga acgacgattc tttagtgttt ttgatccaac catgggttca
      541 ggttcattga tgttaaatgt gcgtaactac ttaactcacc cagataacgt taaatatcat
      601 gggcaagaat taaatacaac gacatacaat ctagcaaaaa tgaacttgat tttacatggg
      661 gtagatgccg aagaaatgaa tctccgcaat ggggatacgt tgaataagga ttggccaaca
      721 gatgaacctt acacatttga tgcagtagta atgaacccac cttattcggc taattggtca
      781 gcagatacga ccttcttaga cgattctcgg tttaatcgtt atggaaaatt agcgccaaaa
      841 tccaaagcag attttgcgtt tcttcttcat ggtttctatc atttgaagga aacagggact
      901 atggccatcg tcttgccaca cggagtttta ttccgtggcg ctgcagaagg ggtcattcgt
      961 caaaaattac tagaagacgg cagtatttat gctgtgatcg gtatgcctgc gaacttgttc
     1021 ttcgggacat cgattccaac gacggtgatt gtattgaaaa agaaccgcca aactcgagac
     1081 gtattattca ttgacgctag tcgagaattt gtcaaaggaa aaaatcagaa taaattatcg
     1141 gaagaaaaca ttcaaaaaat cctcgaaacc tatgctgaaa gaaaagatgt cgagaaatac
     1201 gctcatttgg caaactttga tgaaatcaaa gaaaatgact ataacttaaa tattccacgc
     1261 tatgtagata cgtttgaaga agaagaacca attgatatgg ttcatgtcgg aaatgacatc
     1321 aagaagatac gacaagaaca gcaagtgcta gaaaaagaac ttttagaagc tatctcttcc
     1381 ctgcaaacaa cacctgaaaa tgaagcgtgg ttacaaggtg cgctggaggt gttcaaacat
     1441 gagtaatgat atacaaccag aaattcgttt cccaggattt acggaagctt gggaacagcg
     1501 taagtttgaa tgcttacttg ataaaaaaga tggagtaagg aggggaccat ttgggagtgc
     1561 cttaaagaaa gaattctttg tatctaatag caattttgtt gtctatgaac aacaaaatgc
     1621 tatttatgat aattacgaaa caagatataa aattactgaa aaaaaatata atgaattaat
     1681 ca
//
LOCUS       DASJSZ010000194         1674 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.201, whole genome shotgun sequence.
ACCESSION   DASJSZ010000194 DASJSZ010000000
VERSION     DASJSZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.201"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            39..437
                     /locus_tag="U4B03_002883"
     CDS             39..437
                     /locus_tag="U4B03_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN2069665.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            421..1115
                     /locus_tag="U4B03_002884"
                     /pseudo
     CDS             421..1115
                     /locus_tag="U4B03_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          635 a          242 c          331 g          466 t
ORIGIN      
        1 actattagac aaatgatatt ttacgaggag gaacagagat gacgatcttt gaaacggttg
       61 taacagatgt tggtatagaa gcagaagtat tcaggaaaga aaaaataatg attttatttg
      121 gagatgaagc accggaaaca ttaacagatt attgttataa gattgaattg aatcaggtaa
      181 gaaaagaaat aacttgtgat atgaagctct gttttgataa acaaatgtat gtaatcacag
      241 cagtaggaga tgttgtccaa actaatttga atgaattagg acatatcacg ataaaatttg
      301 atggatctca tgagccggaa cttccaggaa caatgtatgt agaagataag gaaatgccgc
      361 atttaagaaa ggggacaatc attaaaatcg atcaagagga tcagaatggg ggaaagctat
      421 atggaaatca tgcttgatac agcaaataca aaaagtattt attcttgtca acagtttttg
      481 aatttaacag gcgtaacaac aaatcctaca attctgaaaa aagaaggagc gtcaaacttc
      541 ttctcgcata tgaaagaaat caaaaaaata atcggacaga ttcctatcca tattcagaca
      601 ataggaagaa cggaagaaga aatgatagag gatgctcgag ttatcataga agaactggga
      661 aaagatacgt ttatcaaggt gccggtgaat caagcaggcc ttcaagcgat caaaaagttg
      721 aaaaaagaag gttatttgat taccggtaca gctatttaca cagaattaca agggtattta
      781 gctattaata atggagcaga ttatattgca ccttattata acaggatgca aaatcaaggc
      841 attgatgcta tgaaagtgat tgattcgttg cggactgaga tcgaacgtac gcaaagacca
      901 acaaaaatat tagcagctag tttcaaaaat tgtgaacaag tcactacagc cattcgatct
      961 ggagctcagc gataacgtta ggtaaagagc tactagatca agtattagag atgccctcga
     1021 ttgataaagc agtgaggggc ttccaagaag attgggtgca aactttccaa gcagaaacga
     1081 ttggtgaact aaaaaaggtc gctgattact tctgaatcaa tttctccata aaataaacga
     1141 tagccttctc gaaaactcga ggaggctatc tgacgattca atattcatta atttaaagtt
     1201 tgggtgacta ttactaacta ttacttattt ctcatttaaa cgatgtattc cctaacctct
     1261 aatacatcct tgtaactctg aagttacgcc agttttgaat aaatttcata aaaacaaatg
     1321 aaaagtgtcg cataaaaata tctttcgtgt ttctctagta taacagaata agaagaacaa
     1381 gtccagaagg gatattttta gtctttttta aaataacttt tcagtcgagt ttgagtttat
     1441 gatagttgtt tttgaagcga tacggcaagt gaaaaaatag ggaagcatga aagaatctac
     1501 ttaatggcgc attgattcat gtgatagaaa taaaaaactt ttttaaatta tatcctcatg
     1561 attgtaacaa atccagtgat ttgttttggt acaaataaaa aaaggccccg atgaagttgt
     1621 ggcaaaagtg gtttgcctca aaaataagaa ggaatttcca aaaatcactt ctca
//
LOCUS       DASJSZ010000195         1641 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.202, whole genome shotgun sequence.
ACCESSION   DASJSZ010000195 DASJSZ010000000
VERSION     DASJSZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.202"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            81..1604
                     /locus_tag="U4B03_002885"
     CDS             81..1604
                     /locus_tag="U4B03_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEN2069666.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          602 a          269 c          334 g          436 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat a
//
LOCUS       DASJSZ010000196         1613 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.203, whole genome shotgun sequence.
ACCESSION   DASJSZ010000196 DASJSZ010000000
VERSION     DASJSZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.203"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..446
                     /locus_tag="U4B03_002886"
     CDS             24..446
                     /locus_tag="U4B03_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069667.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            456..659
                     /locus_tag="U4B03_002887"
     CDS             456..659
                     /locus_tag="U4B03_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069668.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            870..1454
                     /locus_tag="U4B03_002888"
     CDS             870..1454
                     /locus_tag="U4B03_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2069669.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          572 a          231 c          299 g          511 t
ORIGIN      
        1 ttttgatttc gaggtgatag acaatgaaaa ataagtttat tttatcttta ggtattttaa
       61 ctattggaat tagtgtaact ggaactacaa ttcctacatt tgctgatagt acaaatgaac
      121 caactactgt agtagataat aaccaagaat taactccttt agataggaac ggaatagcca
      181 ttggtaaccc aactaacgac ttaactgtta ctgaaaatta tttaatagtt tctccattac
      241 ttgctcgagc agcttctaaa acgatacgag tgacaggaac gatgattttt gataaagttg
      301 gttggccttc tagacctcct caatcacgga attggaaaga aaaaagaaat ggttattggt
      361 actatggaaa attgtattta caaagttata aatcagctgg aaacaattgg attgcgactt
      421 attcaggaac cttaactaga aagtagggat tttttatgcc agaatttgtg aacacaccaa
      481 atttttatca agatagcgat attctaagta aaattaattt accgaaagaa gaatcagctt
      541 tacaagttca gcgaacttta caggagacac aaaaaattca aaaatcaatt tctaatctag
      601 aagattctga agaaaaaaga aaaaaaagag accaaaaaac atatcaaaaa gaaaaataat
      661 gattttaaaa aaagagtgtc tagtcgacat tctttttttc taattgaaag tgttcataaa
      721 atcacccatt tctggacact ttcttttttt gctttatttt tcaaaaaaac aattcatttc
      781 atgttcataa acccataaca atatctatgt tcagttaaac tatatggata aagttatgtt
      841 acaataaaat taatgaagga gtgatttttt tgaaaatagg atacgcccgg gtatctactg
      901 gtttacaaaa tttggattta caagaagaca gcctaaataa atttggttgt gaaaaaattt
      961 ttactgatca tatgagtggg gcaaaaagta atcgaccagg attagaaatg gccattgatt
     1021 ttgttcgctc tggcgataca cttgtcgttt ggcgcttaga tcgtttaggt agaaatatgg
     1081 aagatttgat ttcaatcgtc aatcgactga atgagcgtgg tgtgagtttt catagtattc
     1141 aagaaaatat tacgatggac aaatcaagtt ctacaggaca gctcatgttt catttgtttg
     1201 cggcatttgc agaatttgag cgtaatttga tcctggaacg atccgcagca ggacgagcag
     1261 ctgctcgtgc aagaggacgg tttggtggtc gtcctgaaaa attgtctgaa caagatttgg
     1321 aattactgaa gactttagtg gatagcggta cacctattaa gactattgcg gaacggtgga
     1381 acgtctcaag aaccaccatt tatcgttatt tggcaaaaat ggaggagaaa aatggaaaga
     1441 caaattctca ttagtttttg tcatttaatg ctgaaaattt ttgccattta tctagaaaag
     1501 tgacaatagt atgagaattt tttgtaagtg tctactgata acttattctc aaatgttgta
     1561 aggattttat tcatttgtta atattaaaga acggaactcc aaaaaagtcc gta
//
LOCUS       DASJSZ010000197         1591 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.204, whole genome shotgun sequence.
ACCESSION   DASJSZ010000197 DASJSZ010000000
VERSION     DASJSZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.204"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            88..1275
                     /locus_tag="U4B03_002889"
     CDS             88..1275
                     /locus_tag="U4B03_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEN2069670.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          527 a          267 c          329 g          468 t
ORIGIN      
        1 taaaagtgaa gcatcaaaaa agccattgat ctcagttagg agtacaatgt tttttgcaga
       61 aaacatactc gaataggact gatatcaatg actcaagtac attttacttt tgaaagcgaa
      121 gaaattcaag caattataaa cgaaagcggt gcgaatgata ccgcaaaaac acttatgact
      181 atcatgttta atcagctgat ggaagaacaa cgaaatcaat atattcaggc aaatgcctat
      241 gaacgttcag aagaacggca atcacaaaga aatggttact acgatcgttc gtttacaact
      301 cgaattggta cgttagaact tcatgttcca cgaaccagag acggtaagtt tagtcctact
      361 atctttgaaa gatatcagcg aagcgaaaaa gctttgatcg ctgccatgat tgaaatggtc
      421 atttctggtg tctccactcg caaagtaact aaaacagttg aactgctaac ggatggtgcg
      481 actgtttcta aatcattcgt ttcaaatctg atgaaacagt tggatccatt tgtttttgag
      541 tggagaaacc ggagcctaga aggctcggaa tatccatttt tcatgtgtga tgccctgtat
      601 atgaaagtaa gggaaaatca tcgtattgtc tcaaaaggtg tatatattgg tatcggcatt
      661 gattctgatg gacgacgtac gattcttggt tttgatgttc aagacggcga atcggaagat
      721 aattgggata ccgtttttca atcgttcgtt caacgaggtt tgttcggtgt aaaactggtc
      781 atttctgatg cacacaaagg gttagttaag gctgttcgca agaacttttt aggtgcgagc
      841 tggcaaagat gccaagccca ctttttgaga aatatttttg ataagctgcc aaagaaagtt
      901 tcttctgatg taaaagacga gttgaagagt atctttaaag cctctgaact tgaattgaca
      961 cgtgaaagaa aagagcactt cttggaaaaa tatggttgtg attcaaaatt aagtgctgct
     1021 tgcgacattt tagaaaacgg ctttgaagat gctattcaaa ttttgtcttt tcctgaaaat
     1081 atccgccgaa ggattcgtac aaccaatgtg ttggaacggt tgaacgaaga gattcgccgg
     1141 agagaacgag tgattcggat ttttccaaat atcaattcca tcactcggat aattggaaca
     1201 cttttgatgg aaaaagacac tgagtggctg gcttctccac gaaaatattt agaatttaat
     1261 tcgaataaca tttagcacca tgaacctgtg cgagtctctt ctttctgagg agagacatca
     1321 agagtcgctg cgctactgct cctgacctct ctcctcagaa agaaaggcat ggcagttagg
     1381 ttcacaacgc aaaacattgt gcttttgatg aggttgaact tttacacaag attgtggact
     1441 tgacttatta atttattaaa aaaagagaaa gaagatttaa atgatgaaat aaatcaaaag
     1501 tatgatgaaa cattaaaaaa tgtctattct tcacttatcg tttcaaatat agaagaaact
     1561 tcatcttctg aaataaaaaa taagctttct t
//
LOCUS       DASJSZ010000198         1572 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.205, whole genome shotgun sequence.
ACCESSION   DASJSZ010000198 DASJSZ010000000
VERSION     DASJSZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.205"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            61..1527
                     /locus_tag="U4B03_002890"
     CDS             61..1527
                     /locus_tag="U4B03_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN2069671.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          437 a          291 c          331 g          513 t
ORIGIN      
        1 aaatttttcg gtaaagatgt ataatatttt ctgaaaattt aaagaattgg ggtatagatt
       61 atggaaaaac agcaagacaa gggcttcttc gggcagccga agggattgtc aactttgttc
      121 ttcactgaga tgtgggaacg atttagttat tacggtatgc gtgcattact tatctattat
      181 atgtacgata cagttgccaa cgggggactt ggattaccac gagcaacagc attagctatc
      241 atgtcgatct acggatcttt gatctacatg tctagtatcg tcggcggatg ggtatctgac
      301 cgagtgttag gagcaaaacg cactgtattc ttcggtggag ttttcattat gcttggccac
      361 atcgtcttgg ctttaccatt tggtgtaagt acattgtttg tttcgatggc attgattatt
      421 atcggaaccg cctttttgaa accaaatgtt tctggcatgg tcggacatct ttattcaaag
      481 actgacttgc gaagagatgc aggattttcg attttttaca tgggtgtcaa cttaggggca
      541 ttattagcac cactaatcgt tggtacaatc ggtcaagaat ataactatca tttagggttc
      601 tctttagctg ctattgggat gttctttgga ttattacaat acatcatcca aggacgcaag
      661 acattagacg gagtgggtac aacaccgccg aatcctttga caactgaaga acgtaaagga
      721 ttcatccgta acttgatcat cgcacttgtt gttatcgctg cagtattcgg cggggcacaa
      781 ctaactggtc atctgacaat tgactttttt gtcaatacaa tcagtgtatt ggggattatc
      841 ttaccgttgt attactttat caaaatgctg acttcaaaag atgtaactgc tgaagaaaaa
      901 ccaagagtat gggcttatat cccattattc cttgcagcaa tcattttctg gtcattacaa
      961 gaacaaggtt catctatctt agctttattt gctagtgaac gtacacaatc tacgctattt
     1021 ggtgtaacga tccaagcaag ctggtatcaa tcattgaatc caattttcat ctttattctg
     1081 acaccagtat tcgtaacgat gtggacgaaa cttggtgaac gtcaaccatc tacagtagtg
     1141 aaattctctc ttggactttt atttgcagga ttatcgtact tgctattgat gttgccgggt
     1201 atgctttatg gtacaagtgg aaaagtaagt ccattgtggt tgttgggaag tttcttcatc
     1261 gtggttattg ctgaattgtg tctttcacca gttggtttgt ctgtgacaac aaaattagca
     1321 ccacgagcat ttgaatcaca aacaatcgct atttggttct tagctgatgc ttcttcacaa
     1381 gcaatcaatg cacaaattgc acgcttctac acaccgggaa cagaatctgc ttacttcggt
     1441 attatcggtg cagtagcaat cgtcggtggt atcttgctat ttactgtgaa agaaccaatc
     1501 aaaaaactaa tgggctctat tcattaatca atgaacataa aaacaggaat tgcttatttg
     1561 cagcagttcc tg
//
LOCUS       DASJSZ010000199         1549 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.206, whole genome shotgun sequence.
ACCESSION   DASJSZ010000199 DASJSZ010000000
VERSION     DASJSZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.206"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            340..525
                     /locus_tag="U4B03_002891"
                     /pseudo
     CDS             340..525
                     /locus_tag="U4B03_002891"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            719..889
                     /locus_tag="U4B03_002892"
     CDS             719..889
                     /locus_tag="U4B03_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2069672.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1313..>1549)
                     /locus_tag="U4B03_002893"
     CDS             complement(1313..>1549)
                     /locus_tag="U4B03_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2069673.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT          488 a          278 c          249 g          534 t
ORIGIN      
        1 gtcattttct tgtcaactat ttttcctatt catgttttta taaaaaaaca tatcgttttc
       61 ttgtatgatt aaatcaccac aaaataaaca actgagagaa cgatatgctt tatgaatggt
      121 tctatcaaaa aaattctgag atcaatagac aaaaatttga tgattacaga ggtctcttac
      181 gaaaccttac agaagaaaaa aacattgatc gttgctgcag ttctcttccc tgctccatgt
      241 gtctgtagaa attgtggttc tgctgtggta gatggaaata ggaaattaat ggtggtgaaa
      301 aacaagaaga aggaaaccat tgtccacttt gaataatata atcatatgcc aatggttata
      361 cgcctaaaaa agcagcgcta tacctgtaaa aactgtcgaa gccattggaa cgctcaaagt
      421 tattttatcc gtccaaggca ctcgatttca aatcatgtca gacataaaat cacttcttta
      481 ctgactgaaa aagtgtcgtt attttttatt tcgaaaagtt gttaggtatc tttgacaacc
      541 gttattcgca cattgaaaga gttgagaagc tatttaccaa agtcttccaa aagaattctg
      601 tcttgagtgt tgagggttgt gaatttcgtt ctcacgtttc cgttgaggat aaactgagtt
      661 ttatttccgc ggatggcgag acagaaaaat taatcgagaa aatttaccta cacaaaaaat
      721 tcctcgatta acaaactatt tccttagctg cactaatcct aaagaaatag aattcttagt
      781 tacagacatg aacgccacct actttcagct caccaaacat gttctaccaa atgcgaaatt
      841 ggtgattgat cgattacttg gtttttccac gcctttcaag gaggcgtaac ctttattcac
      901 gagttggttg aagctttccg agacaaaaga cccgacttgt ttttctcctt attgacaaag
      961 agccattaaa actagagaaa aaattacaac tgatcgctga ttatttgtag aaagaaaatc
     1021 aaagtattac ccaataaact tgagtcatta ggcaaaaaac actgcgaaac tcaagaatga
     1081 ttttcgcagt gtttttgtat attttttatt aactagtatt actacaaagg tttatttttc
     1141 acttctcaac gcagcacaaa attgtcttta cttatttact ggttgggttc aaccttctct
     1201 actccgatac taatatttgt ttgtataccc cttctaaaat cggtagtttc cattcaattg
     1261 atgggtacta gttgatttta aaaactatgc ttattcaatt ttttatttta gctcatatat
     1321 ttcgtttctt tctaaatttt atactgatga aaacaagtag cacacccaat attgacaata
     1381 taaactggct aatgatggtt tctccagttt ttggaagatg cttatttcca ctgcttgttg
     1441 gattgtttcc tcttatatct gagctatcag caacttttct attctctttt gttaaaaaga
     1501 tcgccacttc ttgttctttt tcgatcttga aagttactgg tgtcgcatc
//
LOCUS       DASJSZ010000200         1537 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.207, whole genome shotgun sequence.
ACCESSION   DASJSZ010000200 DASJSZ010000000
VERSION     DASJSZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.207"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(313..386)
                     /locus_tag="U4B03_002894"
     tRNA            complement(313..386)
                     /locus_tag="U4B03_002894"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(350..352),aa:Ile,seq:cat)
     gene            complement(575..1534)
                     /locus_tag="U4B03_002895"
     CDS             complement(575..1534)
                     /locus_tag="U4B03_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN2069674.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          454 a          301 c          276 g          506 t
ORIGIN      
        1 gtatgctttt taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggca
       61 gtggtttttg gggaacgctt taaactcttc tttattatta tctcacaata taacccaaat
      121 gtctttctat ttaaaaatca aagtaaaact tttcaaatat acagaaatat aactatgtga
      181 aacatccttt cattaatcca taaaaggata cataaaaaag ccactcattt gagtggcaat
      241 gaagaaaagc tttagcttgt ataatactct tcaaaaaatt ctaacacaga acgattcaaa
      301 tggctacgtt aatgtaccct gtaggactcg aacctacgac cggacggtta tgagccgtct
      361 gctctgacca actgagctaa gggtactggt tgttgccaca taaagccata aacaatcaac
      421 cagtagaatg tgtggcaaca aacctgttat cgcatagctt ggagtgtgac tattatgggt
      481 gatagtgaag atatgcgata acatcactat tttatcgaat gatttttata gttgtcaata
      541 tagttatgta atgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc
      601 aacgtttgtc atggaaatct tgcgcaccgc ctttagtgtt tccctctgga tcattcgttg
      661 cccgcatcat gacatagact ttcttattag ggaagttacg catgttaaaa gatacatgat
      721 agccaacatt tccagaagta ttataagctt gatttacatc tgctctataa attccatcag
      781 ctcttactcg agctaattct ttcccagtat tgtaatccat aatgaagata tactcgtatt
      841 tatagttagc aatgtgccat ccagctacat gcaaatttgc attttcgatt tctccaaact
      901 gatcaatgtg ggcgtaattc gttccatctg tcagtgtagg attagctgca ccagctcgtg
      961 ttggatcaat gacaggcttg ttttcagaag ttgttggatt ttcatcggta aatccatgag
     1021 ctaaatcata tgctaatttt tctttactta cgcccatttc agaaagataa ccgtaaggat
     1081 ctgtatgatc gccccagatg ttttgtgtta cccataaatg cgatttgatt cccggttggt
     1141 tataaggagt gtccaacgtt aatggaatac catatttcat tgctgaatct ctagccaatt
     1201 caacgtatgc cttgtagttt ttctcaaacg ttgctttatc atgtgtgtgt tgtaactcaa
     1261 tctgcacagg actgttggca ttagcatacg aaccagcacc gtactgtaca taaccaggtt
     1321 gaccgacttg ataaacaatt ccgccgtctc ccacaatata agcagtataa gcgctagtcc
     1381 atgaacgttg catatactgc gcttcattgc gtcctgttgc tgtttcatta gccgtttcat
     1441 gcagtaaaat atactgatta tttgctactt gtgagctacc ttcatttgcg cccaaattaa
     1501 attcattgtt gatagtatag gcaaatccgt taattgg
//
LOCUS       DASJSZ010000201         1529 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.208, whole genome shotgun sequence.
ACCESSION   DASJSZ010000201 DASJSZ010000000
VERSION     DASJSZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.208"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(158..1465)
                     /locus_tag="U4B03_002896"
     CDS             complement(158..1465)
                     /locus_tag="U4B03_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN2069675.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
BASE COUNT          416 a          308 c          269 g          536 t
ORIGIN      
        1 tattcacctt ttgatagcca agcttttcca tcattataaa accgcaaatt tcaaaaaaaa
       61 acgaacaata caaaaagcgt attgctcgtt tttctcttca gtttctcaca cctaaacgac
      121 ttttgtctcg acctcttttt ggtgactatt catgatgtta aaataaatta ctgaaaaaat
      181 ctgtgacatg gcgccaacca atgacaaaaa tattagcttt ttcaacagta gaagtggtaa
      241 tgatgtctgt cgaaggctct tggtttgctt ccaaatagcc taatttgtca tcagctaatt
      301 ggatcgttgc tgagccaact gtagtgcctt tgttgatagg agcggtcact tggtcatccg
      361 ttactttctt tttatcaatt gttggagtaa tcgttaattt tccagtatcc attccatttt
      421 cgacccagac ttttacaggg ctttttagcg caacattgac ggaggtttct tttccatctt
      481 tcactttgat gtcttttagg cttgggatgc ttgcatttgc ttttccaagt tctttttctg
      541 accagttgtc ataacaataa tccatcaatc gtgatgtttc attgaatcgg gcacttggat
      601 tttcttcatg gttatctgca tttaaaacga ctgtgatgat tcgtcggcca tctttgttga
      661 tcgttccgac aaagcatgca cccgcaaaat cagttgtgcc agtttttaat ccatcgacac
      721 cttcgtgata gtttacaaaa ccaggtagca tccagttcca attgaccatt tcaaccggtg
      781 actgtgtgtt ttcgccgaat gtttgatttg tggtactcgt gatttttaag acttcaggat
      841 aatctaagat gaggtgacga gcaacaatag cgacatcttt cgcagacagt tgattttcat
      901 ctgtttcacc agtattctca ggtcgattct cacctaggta agaattgttt aaacctgatg
      961 cattgacgat cgttgcatct ttgatacccc aatcttttag ttgtttcttc attagctcaa
     1021 gaaatttact ttcactgcct gcaacttttt cagctaaagc aaccatggaa gcattggctg
     1081 attggatgaa agctgagtca acaagttctt tcactgtata atcattttct ttatgcaaag
     1141 gaacattcga tagatccggc acgacactta atttttctgc ataatccgaa atggctacct
     1201 tatcttccca agataattta ccctctttga tttgttcaag tacgatatat agaccaatga
     1261 tctttgtgat ggaagcaatt cccatcgttt tttcgccatc ctgatcataa aggatttttc
     1321 cactttctgc atctacggca aaggcagctt tggcatttat tttaaatgcg tcgtctgctt
     1381 gaacagttgc gctaggcaag aaagaagtga tacataagat aagacttgct aatagtacaa
     1441 taaaacgggt attttttttg aacatctttg ttctcctcat ttttaaattg attcttacaa
     1501 cttagaatat tatcagagag aacaaaagg
//
LOCUS       DASJSZ010000202         1500 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.209, whole genome shotgun sequence.
ACCESSION   DASJSZ010000202 DASJSZ010000000
VERSION     DASJSZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.209"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            124..756
                     /locus_tag="U4B03_002897"
     CDS             124..756
                     /locus_tag="U4B03_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN2069676.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            879..>1500
                     /locus_tag="U4B03_002898"
     CDS             879..>1500
                     /locus_tag="U4B03_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2069677.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILL"
BASE COUNT          503 a          228 c          242 g          527 t
ORIGIN      
        1 aacgtttcaa gagatagcga cactatcgtt atcgaaaaag gcgaaaacct atctcatttt
       61 ctgctaatgt aagttggttt agcttttgtt aatatgactt tatgaaaatg gaggtaatta
      121 aatatgattt ttcctaaagt taatgaagca agcttgaata aaggatcctt ttttcatatc
      181 tatgaaaacg aagattttga actgtataat atcctttcac aaaaagaggt catcgaaaac
      241 caaataatct gccatccaat tatttacaaa tctattttca agtatgcatt ttttgcttcc
      301 aatgattttc aaagaatgga gattaccgac tgtatattcg aaaactgtga tttttcaggc
      361 tgtatattga caaaaagcat gttgcatcgc gtgaaatttg tcaattgtaa attaacaggt
      421 actaaattga tggaaagtta tattgggaac acgttattcg agaattgtaa aatggactat
      481 gtaaatttat ctggttcaaa cataaaagaa agtacctttg agcactctgt cttatcaagc
      541 gcagattttg tagactgttc cctcactaaa ataatgtttt ctactaatga tttagaaaat
      601 gctagctttt ttgatactga tttaaaaaat atcgatttaa gtagcaactc ctttgaaatg
      661 attgaagtac gctcggaaca tctaaaaaat tgtacgctta atcagttgca agcacttggt
      721 tttgctagaa aatttttaca aataaaagta gtataatcaa aatgaatata taagataaat
      781 ttttagcaat acaaatatcc ataaaaaaac attgcctatt ttaactcttc atgtatactg
      841 actctatctt caaaaactga atagtaggag ttattatcat gaataacatt ttaggaaaaa
      901 aaatagaact taaaaaaggg atattgggca ctattgtcgt tgtactcggt ttgttagggt
      961 tgctatttgc gccatccatt attagcattt tttctttaac tatcactgga tttatagcat
     1021 ttacacttgt ttttggtaaa aaagatacga aaaaaatgtt ctcgaaacct gtcgctcctg
     1081 tcaaaaatat agccaaatat ttttttatca atgtagtaat cagtgccgca gtctcggtat
     1141 ttttgcaaca aattttgaaa tggggtttaa caggcaatcc aataaatgag gctttcagcc
     1201 cgttattatt tattattttg cctatcatga tacttggaga agaattgttc tcagtgtatt
     1261 ttctcgctat tttttctagt aagttcagca ttcctgtcgc tagtttcttg tcagcaatca
     1321 tctttggatt tatccattac agtacttatg ataatggaaa tattttacac actgtagcac
     1381 acatcctttt gatacaaggg gtggcaagat tgttattcaa ccaagctgcc attaaaagca
     1441 actcgatcat tacaagttgg gcagttcatg tgatatttga tttcacagct atattgctag
//
LOCUS       DASJSZ010000203         1480 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.210, whole genome shotgun sequence.
ACCESSION   DASJSZ010000203 DASJSZ010000000
VERSION     DASJSZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.210"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            64..972
                     /locus_tag="U4B03_002899"
     CDS             64..972
                     /locus_tag="U4B03_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN2069678.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="U4B03_002900"
     CDS             972..>1480
                     /locus_tag="U4B03_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069679.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DASJSZ010000204         1471 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.211, whole genome shotgun sequence.
ACCESSION   DASJSZ010000204 DASJSZ010000000
VERSION     DASJSZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.211"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            86..496
                     /gene="spx"
                     /locus_tag="U4B03_002901"
     CDS             86..496
                     /gene="spx"
                     /locus_tag="U4B03_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN2069680.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            510..866
                     /locus_tag="U4B03_002902"
     CDS             510..866
                     /locus_tag="U4B03_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEN2069681.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          541 a          209 c          271 g          450 t
ORIGIN      
        1 ttttttttat attttattag ctaataattg gtattttaaa agaaattact gtagaattgg
       61 attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta gctgtacttc
      121 ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag agaagaatat
      181 ttttgccgaa ccgttaacag aaagtgagat caaaaagatc ttggcagcga ctgaaggagg
      241 agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca tagattttaa
      301 cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat tattgagacg
      361 acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg agatccggtc
      421 atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac aaaacaataa
      481 catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga tcagcgcact
      541 gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct tatcaatttg
      601 ttgacttaga agaacgcgat ttaagtacag aagaagcaga agaattatgc agttttgaag
      661 aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac attgcgattg
      721 acctttcctg tgtaggaaaa aaacaactga tttttttatg cacacaacat accaatctcc
      781 tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat gagcagttga
      841 tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca ctgagcattt
      901 catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct ttttttgact
      961 ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa tctaggtggg
     1021 gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc atttctgttg
     1081 ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag ttgttgacat
     1141 gttagaaatg acatgataag ataactgagt tgttaaaaaa gaaccacttg tgaagcttga
     1201 ttctatcgaa aaaaacttca aaaaagttct tgacaaataa caaatgataa gttatgataa
     1261 atgagttgct gtaagatttc gaaaaacgaa ataaaaaaaa cttttaaaaa agagttgaca
     1321 attacttgtc tctctgatat gatataaaag ttgctacggc agccaaaaac gaatcgggga
     1381 acactcctga tgaagtagac ctttgaaaac tgaacaaagt aagacaaacc aaatgtgtag
     1441 ggcgtcttga ttcaattcaa gacaacaaac a
//
LOCUS       DASJSZ010000205         1445 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.212, whole genome shotgun sequence.
ACCESSION   DASJSZ010000205 DASJSZ010000000
VERSION     DASJSZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.212"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            221..655
                     /locus_tag="U4B03_002903"
     CDS             221..655
                     /locus_tag="U4B03_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2069682.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            678..998
                     /locus_tag="U4B03_002904"
     CDS             678..998
                     /locus_tag="U4B03_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069683.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1139..1330
                     /locus_tag="U4B03_002905"
     CDS             1139..1330
                     /locus_tag="U4B03_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069684.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          564 a          212 c          305 g          364 t
ORIGIN      
        1 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
       61 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      121 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      181 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      241 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      301 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      361 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      421 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      481 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      541 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      601 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
      661 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
      721 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
      781 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
      841 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
      901 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
      961 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1021 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1081 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1141 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1201 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1261 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1321 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1381 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1441 agaaa
//
LOCUS       DASJSZ010000206         1445 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.213, whole genome shotgun sequence.
ACCESSION   DASJSZ010000206 DASJSZ010000000
VERSION     DASJSZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.213"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..356)
                     /locus_tag="U4B03_002906"
     CDS             complement(<1..356)
                     /locus_tag="U4B03_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2069685.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMA"
     gene            complement(338..1327)
                     /locus_tag="U4B03_002907"
     CDS             complement(338..1327)
                     /locus_tag="U4B03_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2069686.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
BASE COUNT          474 a          267 c          185 g          519 t
ORIGIN      
        1 gccattaata actccagtaa aagaggtaaa ttaatcccag caataatttg aattctattg
       61 ttgttcaact gtttaataga aacattatta ggtgttcctc caattaaatc cgttaaaata
      121 attagaggtt gcaaatgatg ctgactaatt atttctttca tactctcctc aaaatcatct
      181 ggactagtat tttctttaaa tggaacagca ctaatacctg tggcgtcatt cattaaaatt
      241 aattttgttg cctttaaata accctcacaa agggtagaat gtgttgtaat cacaatgtga
      301 aataattgat ttgccatctt ttgcaacctc ctattaatta atatttcatt tgccacatat
      361 agcgtctaac atctaaggaa tggcctcttt caaaagcaat cgcttctacc atttggcgaa
      421 taacaacgcc actaagtaca gctgcaatat attctttcgc tacactatct atagattcta
      481 agtcaaaatc agcttggtct aaaaccatta atttattact gaatttttta gtgaagtctt
      541 ctacccgttg atctaaaaat cttgtatctc caatactttt cactaagatg aatggtacat
      601 cataatcagt gatttcaaaa ggaccatgga aaaattcccc agaatgaata gcattagaat
      661 taatccattg catttccatg aaccagcaag ctgctgtgga gtaaatttct ccgtaattga
      721 taccgctacc aattgtgtaa ataattttat ctcgtttttg gtttttaccc cattctttag
      781 cagttatttc aaatttgctc ttttcagcaa taacaagatc atttaactta tcaaattcat
      841 tcaagcaagt ttcccatttt ttagagccat ctaaaacaag taaaatatta aataataaac
      901 cataaagaat tcctttattt aaatcactag catcaacatc tttaccccaa tcatagtgaa
      961 caggatattc agctgcttcc catagaggag atccttcttc attagataaa caaatagtca
     1021 ttgctccaaa ttctcttgct ttttttgttg cttctacagt ttctggtgtt gtgccagaat
     1081 gggaacaagt gataactaaa gaattttcag ataatccttt tggtatagca tagttaaatt
     1141 cgttagcagt ataaacatga caaggtacag ttaattcttt atcaaataga tattgaccag
     1201 ccattaaaac ggcttgtgaa cccccacatg ctgtaaaata tacatgatca attgtctttt
     1261 cctttaacgc tgccattgct gtttcaatct gttctttgtg tagttgtctc atttctttat
     1321 ccctcactct aataagtatt ataaagtcat ataactttac aatacctatt atctatgaaa
     1381 ttgctttatt ttgcaatact atttttcaaa aaaatttttt tataacaatt ttatataata
     1441 aaatg
//
LOCUS       DASJSZ010000207         1393 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.214, whole genome shotgun sequence.
ACCESSION   DASJSZ010000207 DASJSZ010000000
VERSION     DASJSZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.214"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(364..978)
                     /locus_tag="U4B03_002908"
     CDS             complement(364..978)
                     /locus_tag="U4B03_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2069687.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          454 a          270 c          232 g          437 t
ORIGIN      
        1 taatgataac aaatgtacta taattacttt gtaaagagtt ttggggaaga ctctttcatc
       61 aacgtgtatt aagagacaag aaagaatcca tatggattct ttcttgtctc tttttccact
      121 actttttatt tttctgacac tacatattct tataactcat ttctcataac tgtgaaacaa
      181 cttataaaaa taacaaactt taaacaaaac aagcttaaaa tcaaagatga tccatcttaa
      241 aataactaaa acaaaaaaag tagtcgttgt taatcctcat taataatcgt tttctttttt
      301 tcaaacgctc catttttatg tggaacatta tttttttaag gaactttgtc aacatgtgga
      361 taactatttt ccacttgtgt cctggactag ctgttttgct gtaaaataca aaacttgctg
      421 cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc gctggatcta gatttggcaa
      481 agtcaagact gcatcatgta aattggcgtg gttgtccaaa accaaatcag caaattcaaa
      541 taatcgcaaa ccactgctat gttgcgattt gatacttcgt gacaaatcaa atccagtaac
      601 aatgattaat ggatgaccat tttctttgat ccactgagct agttcgatga ttgccgggtt
      661 tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa gctgatcaat
      721 aattgcctgt cctgcacctt ctagcgtttc atagacacct ccggctggat tttcaatcaa
      781 tatacttggt acaagatccg gggtttgatc ccaaaattca aatgctacac cacttagtgt
      841 gcggctagca tatatctgca atctaccacc ttgattaatt tgtttcgcga tccaagtgcc
      901 agcttgaagc atcagcggct cttctatcct taatttctct tccaattggt gataatagtc
      961 aaaataacat aaactcattt atctctcccg cctagctata cttgacatga atagaaacag
     1021 ctgcctaatc taaggaagta cataaaaaaa cagtagttag acagtaacac atgtggtctt
     1081 tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc caataccttg acatagcaaa
     1141 taaaacaacg tgcagaccat ggaaaatatc tacatagggg tagaattttc accctagatt
     1201 aggtacaata aattaggtcg atgcactgca ttgactatgg caaaatgatc ggtcagggtg
     1261 tatgtgtgtg actggtcatt ccctgattct tagcgccaac taagggtcag ggagcctttt
     1321 tttattcagc tgtttctatc tgaatttatt gtaagtaatc tcttatccaa gtgcaagcaa
     1381 gaaaatacaa aaa
//
LOCUS       DASJSZ010000208         1374 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.215, whole genome shotgun sequence.
ACCESSION   DASJSZ010000208 DASJSZ010000000
VERSION     DASJSZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.215"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..1372
                     /locus_tag="U4B03_002909"
     CDS             join(33..474,474..1372)
                     /locus_tag="U4B03_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEN2069688.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DASJSZ010000209         1364 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.217, whole genome shotgun sequence.
ACCESSION   DASJSZ010000209 DASJSZ010000000
VERSION     DASJSZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.217"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            80..>1364
                     /locus_tag="U4B03_002910"
     CDS             80..>1364
                     /locus_tag="U4B03_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEN2069689.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          465 a          263 c          257 g          379 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccctttttt catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc
     1141 cttattggca gaacttcccg aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct
     1201 tctgacctat gaagaaggca tcaccaacgc aatgatctat ccttattcca atggaaaaat
     1261 agaagcgaag aatacccaca taaagacaat gaaacgagta tcctacggat ttaaatcatt
     1321 tgagaacatg agaattagaa tctttttgat caatcaatta atca
//
LOCUS       DASJSZ010000210         1355 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.218, whole genome shotgun sequence.
ACCESSION   DASJSZ010000210 DASJSZ010000000
VERSION     DASJSZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.218"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1355
                     /locus_tag="U4B03_002911"
     rRNA            <1..1355
                     /locus_tag="U4B03_002911"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DASJSZ010000211         1336 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.219, whole genome shotgun sequence.
ACCESSION   DASJSZ010000211 DASJSZ010000000
VERSION     DASJSZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.219"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..1145
                     /locus_tag="U4B03_002912"
     CDS             6..1145
                     /locus_tag="U4B03_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEN2069690.1"
                     /translation="MLINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFNS
                     GNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKGI
                     TMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPLK
                     RQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVTD
                     GLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLVYQASSKEEAN
                     NQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKYTK
                     RKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          481 a          193 c          225 g          437 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta gtttaccaag caagttcaaa agaagaagca aataatcaaa
      841 taaggtttat gatagataaa tggaaaaagc agtatccacg agtggttaaa ttgctcatga
      901 atcctgctat attaaccttt tataatttcc ctccgtcaat cagaagaact atttattcaa
      961 ctaatttgat tgaaggtttt aacaaacaat taaagaaata taccaaaaga aaagagcaat
     1021 ttcctaatga agaatctctg gagagatttc tagtatctca attcaacaac tataatcaaa
     1081 agtttctatg ccgagttcat aaaggtttta aagaaataca tgatacatta gaatcaatgt
     1141 tttaacttga tcaacagaat gggcttttcc atttacacaa aattattgac gctcccgaca
     1201 aaattatgat cttgcgtaaa gttataaaat attaataaaa atattttatt aatgaaaagc
     1261 ttggtagcac ggttattcat atgctaattt atttcacttt atagtaaggg gagttcttag
     1321 tatattgttc aaactt
//
LOCUS       DASJSZ010000212         1328 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.220, whole genome shotgun sequence.
ACCESSION   DASJSZ010000212 DASJSZ010000000
VERSION     DASJSZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.220"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..484)
                     /locus_tag="U4B03_002913"
     CDS             complement(<1..484)
                     /locus_tag="U4B03_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2069691.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     L"
     gene            complement(474..>1328)
                     /locus_tag="U4B03_002914"
     CDS             complement(474..>1328)
                     /locus_tag="U4B03_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN2069692.1"
                     /translation="IWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWIDLKYNAIT
                     TWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWT
                     NLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNI
                     KNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVN
                     GISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGA
                     VGGTLYAT"
BASE COUNT          425 a          235 c          159 g          509 t
ORIGIN      
        1 taagcggttt tgaagaatac ttctctgggt tataaatttc aaaagtttct gtttgtcgaa
       61 tagtaaatct accattgtaa ttttccttaa atggtcgaat actcactgta aattcaaaag
      121 ggacaatatt tcctgttttt cttgttcctt taaattcagg tccttctatt actacagctt
      181 gataaatata ttgttcatca tagtaaagaa taaaatcact ataagacgac atatctaacc
      241 attcagtgat tcgatcttcc cactcctgca ccatatcaat tgaaggtgct ttatagtaac
      301 actcaatctt tctagtcaca tttttgtaat agcctttatc aataatgata gaatcatttc
      361 cttctcgctc tctcaattca attactcgac tagctgaaac agaagcaggc ctgttttgaa
      421 tatacacatt aaattcagaa gaataatgtt gattaataaa aaattgtcct ctcttaagtt
      481 gcatataatg ttcctcctac tgcaccagca tctcgtttca tttgtcttgt caattctgtt
      541 ttaatttttg tagtaatctt tcgaaccatt gaatcaggta aatctccata aacatttaaa
      601 tgtaaatgaa tttcttttgt ttcactagca gatatatttt gctttgtttg tgtaattggt
      661 aggtgactta ttccatttac tacaggttga acagacggtg tttttactaa atttgtcatg
      721 gtttgatcta acttactttg ctctttatca ataccaacga taataccttg aacaatattt
      781 ttaccaacca tatcacgcat ccatcttgat ggagaatgaa tatctaaagc tcctttaatc
      841 caatttttga tattacctgc aatacctttg atagtatctt tcaaagcatt ccacttttct
      901 tttacaccat ttataagtcc atcaatgatg ttcttgccaa tttcaaataa attgacttcc
      961 cttaacgaat taaagatttc ttttacccga ttaatagcat ttgaaacacc attttttaga
     1021 tttgtccatg cattttcagc agaattaaca attccagtaa caatattcca gaaagagtcc
     1081 ttaatgttat tccatgtatt aatcatggta ttttttatag aaatccacgt attgtatgcc
     1141 gtatctttaa tatttttcca agtatctttg aaaaactgtt taatattatt ccaagtagta
     1201 atggcattat attttaaatc gatccaagtt tgaataatag aaaacttcaa ttcaatccat
     1261 ttctgaattg caaaatattt tatatcaatc caaagattaa taaaaaaata ctttatgttt
     1321 aaccaaat
//
LOCUS       DASJSZ010000213         1276 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.221, whole genome shotgun sequence.
ACCESSION   DASJSZ010000213 DASJSZ010000000
VERSION     DASJSZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.221"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..863)
                     /locus_tag="U4B03_002915"
     CDS             complement(<1..863)
                     /locus_tag="U4B03_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069693.1"
                     /translation="MVAVYEYLKKNIPGCDDNVNASNRMFFGGYKSDAVIIINEDNML
                     DITELPIDFTMTCSKNCQSDSGREYELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSD
                     MSFNEIYERLLTIDMNKLLKSSKNLRCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDI
                     SENFIGVVMLILKKDSRVETFNWLLENLDLYPTHFRKLKEESKVLFDTLESMKDKLFR
                     TIRNYLEEMRRVYDVLLSDVNFFDDSKESVTCILSGEQLAKKMSSYYGKTYDIDKCNK
                     LLSLMTFIGLI"
BASE COUNT          401 a          248 c          150 g          477 t
ORIGIN      
        1 tttatcaatc ctataaaagt cataagtgat agtaacttat tacatttatc tatgtcataa
       61 gttttaccat agtaagaact cattttttta gctaattgtt cccccgacaa aatacaagtc
      121 acactttcct tactatcatc aaaaaaatta acatcagaaa gcaagacatc atagacacgt
      181 cgcatttcct ctagataatt ccttattgtc ctgaaaagtt tgtctttcat tgattcaaga
      241 gtgtcaaaaa gtacttttga ttcctctttc aattttcgaa aatgtgtagg atacaagtct
      301 aggttctcta atagccaatt aaatgtttct acccgactgt ctttctttaa aattagcatc
      361 acaactccaa taaaattttc actgatatca caattactgg aatgacaata aaaaatggaa
      421 tgcccagttt tactggtaaa aatactagca gagggattat gatcgttgtg aaacagacat
      481 cttaagtttt tacttgactt aagtaattta ttcatatcaa tggttaacaa tctttcataa
      541 atctcattaa aagacatgtc ggaggaatca aacaggcagt ctttaaacca ctgtttcatt
      601 tcttctttgt cgtcattttt tatcaactta acaaaatcgg tttcattgac tagttcatac
      661 tctctccctg aatcactttg acaattttta gaacatgtca tggtaaaatc aatcggaagc
      721 tcagtaatat ctaacatgtt gtcttcatta attattatca ctgcatctga cttatatcct
      781 ccaaaaaaca ttctattcga agcatttaca ttgtcatcac acccggggat gttctttttc
      841 aagtattcgt aaacagcaac cattttacca tagtcagtaa ttgcttcttt taatagaaag
      901 acaatcttgt atttttctag attttcagag taccgaatac ttttttgtat cgcaaacgca
      961 gaattatgaa ctaaattgat agataaaacc ttttctatgc ttaagtatct actatcatct
     1021 ttactcaaat ctactacctt ccccttctca tctttaacag tgttatcgac atccagaata
     1081 attactctgg tttccacaaa actctccttg tcattgcctt tattctttgt cttgcttgga
     1141 ataaatgatt gtgatgaatt tagtatctct acaaactcat ctcttgtaac ttctctatag
     1201 ttatctaaac acctatctct tacatcagtt ttttcttttt ttgtaactgg cttctctaat
     1261 aatttttctt tatcta
//
LOCUS       DASJSZ010000214         1271 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.222, whole genome shotgun sequence.
ACCESSION   DASJSZ010000214 DASJSZ010000000
VERSION     DASJSZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.222"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..870
                     /locus_tag="U4B03_002916"
     CDS             <1..870
                     /locus_tag="U4B03_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEN2069694.1"
                     /translation="NKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIKIFD
                     KINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRRE
                     IDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIREN
                     VLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAM
                     IFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAK
                     AEQYRRSLELSKD"
     gene            942..>1271
                     /locus_tag="U4B03_002917"
     CDS             942..>1271
                     /locus_tag="U4B03_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069695.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNS"
BASE COUNT          564 a          146 c          207 g          354 t
ORIGIN      
        1 aataaacgag agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg
       61 aaaggattac tcaataaact aactatttct aatatagact cgactaggga aaaaatcata
      121 aaaatatttg ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt
      181 cttttagagt ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc
      241 gaagaaaaag aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt
      301 agagaaattg atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa
      361 atttccaaat taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat
      421 gcaaataaaa ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat
      481 gtattacaaa gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg
      541 tcttttggtg aagacatata taaaattggt atgactagaa gattagaacc tatggataga
      601 ataaaagaat tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt
      661 tcagaggacg ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt
      721 aataaaatta atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt
      781 gttttagaaa attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat
      841 agacgatctt tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt
      901 taacaattaa taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa
      961 atgacaaaaa atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa
     1021 aagtgtccgt tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt
     1081 tgaatgaaaa aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag
     1141 aattaaaaac taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa
     1201 caactcaggt ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa
     1261 acgttaatag t
//
LOCUS       DASJSZ010000215         1247 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.223, whole genome shotgun sequence.
ACCESSION   DASJSZ010000215 DASJSZ010000000
VERSION     DASJSZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.223"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..717
                     /locus_tag="U4B03_002918"
     CDS             <1..717
                     /locus_tag="U4B03_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEN2069696.1"
                     /translation="LSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPV
                     LLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGY
                     SDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIF
                     LGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYI
                     GKKVKRPISADSVLLEEDVE"
     gene            714..>1247
                     /locus_tag="U4B03_002919"
     CDS             714..>1247
                     /locus_tag="U4B03_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEN2069697.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDT"
BASE COUNT          412 a          167 c          271 g          397 t
ORIGIN      
        1 ttgtctactc cttttgatga agaaagtgca gactttctaa aacaaattgg tgtacaagga
       61 tttaaaattg gctcaggaga tttaacaaat ttaccttttc ttagaaaaat agatatgtat
      121 gggttgccag ttttattatc tacaggtatg gctgatattc atgaggtcag tgaggctgta
      181 gattgtttca tagatagtcc agttactatt ttacattgta cttcaaatta tccggctagt
      241 tctgaagata ttaatttatt ggcgattaac acattaaaag aaatgttcgg gatacccgtt
      301 gggtactccg atcattcatt gggatatgat gttggtattt gtgctgttgc gatgggtgct
      361 aaggtaattg agaaacattt taccttggat aaggaactac ctggtccaga tcataaagct
      421 tctttagatc ctcaggaatt tgcagatttt gtaaagcata ttagaaacac tgaaatattt
      481 ttaggtgatg gtgttaagcg agcaatgcct tctgaactta gtacaaaaga agtatcaaga
      541 aaaagcctag tggtcactga gaatttaaat gtgggagata ttcttacaga gaaatctctt
      601 acaataaaac gtcctggaaa tggaatagct cctaagtttt tatatcaata tattggcaaa
      661 aaagttaaaa gaccaataag tgcagatagt gttctgcttg aggaagatgt tgaatgaaaa
      721 aaatacttat agtaagtgga acacgagctg attatggaat atataaacct gttgctgaga
      781 atttactaca agataattgg gatgtatcat ttgttgtttc tggaatgcat ttatcccata
      841 aatatggatt tactaaagaa ttgatcttag ctgacggatt tagaattagt ggagaagttc
      901 aaagtttgtt tttagagaac aataagggaa acatggctcg gtctgtggca ttagagataa
      961 atggtttcac aaatatattt gaacaaacta gccctgattt tgtattatta ctaggggata
     1021 gaggagaaat gttggcagct gcgatagctt gtttgtatct tggaatcaaa acagcacata
     1081 ttcacggagg cgaagtgtct ggtactgtag atgaatcaat aagacatgcc ataacgaaaa
     1141 tagcatctgt tcattttgct gctacggaaa aaagcaaaga gcgtttaata aaaatggggg
     1201 aagattcttg gagagttttt cccgtcggag ctcctagaat agatact
//
LOCUS       DASJSZ010000216         1215 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.224, whole genome shotgun sequence.
ACCESSION   DASJSZ010000216 DASJSZ010000000
VERSION     DASJSZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.224"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..969
                     /locus_tag="U4B03_002920"
     CDS             <1..969
                     /locus_tag="U4B03_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2069698.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          404 a          220 c          247 g          344 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttc
//
LOCUS       DASJSZ010000217         1209 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.225, whole genome shotgun sequence.
ACCESSION   DASJSZ010000217 DASJSZ010000000
VERSION     DASJSZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.225"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            129..1169
                     /locus_tag="U4B03_002921"
     CDS             129..1169
                     /locus_tag="U4B03_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN2069699.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          487 a          176 c          204 g          342 t
ORIGIN      
        1 ctgattttag gtagtattta attaaaaaac agttttaatg cgtttgcatt ttttatttat
       61 ctttgctatt ttcgaaaaga gtaggtaatt ttacataaat aaaacaacgt gaaagtagag
      121 gatctacaat gactgatttt aaatttttta aggcggatag agtttacaac gaattatttt
      181 atcaatttcc aaaagtcttt attgtttctg acgaatacaa aaaaatgaaa gattcaacta
      241 agattgccta tatgctttta aaagcaagat tagagatcgc aatcagcaaa cggcaaatcg
      301 atgaagaagg taatgtttat tttacttata cgacaaatga actatgtaga gtattaaact
      361 gccaaaaaca aaaagcgata gcaatcaaaa aagagttgga atcctttggt ttattattac
      421 aaaagcagat gggatttaac aaacagttag ggaaaaataa tcctaataga ctatatctag
      481 cagaattaaa agtctcagaa aatgatatct acttactcga aaaatttgat agagagaata
      541 gggaaaacgt tgataaatca gagggtatga aaatcatacc caccctcgac gaaaaatcag
      601 acgctgaatc ccttggggct caagagggta tgaaaatcat accgtgccaa aacgttgata
      661 aatcagaggg tatgaaaatc ataccagaac ttaataataa tatattagac actaatagac
      721 acaatataga cactgaaaaa gaccgcctac aagatcaatt gttgttagac aattttgaga
      781 caattatgac aaacgacagc attgctacgt ttgtccctga acgagtatta aatttgataa
      841 aaacattttc ttcaagttac agtgaagctc aaaaaaccgt ccagactatt cataatgcaa
      901 agaaaaaagc tgaaatagaa agtgatattt cgatagtttt tgaagaactc gatagttatt
      961 atgtcaatgc agaacaagaa ttatacacga cactgttaaa agcctatcaa aaattaaaaa
     1021 ccgaaaaagt cgaaaatatc cagaacctga tttttgtcta tgtaaaaaat tggtttatcg
     1081 aaaaaccaat agctgctaaa gtatcaagtg aaaaacgttt gaattatgca agctccccaa
     1141 gcactattac gaaagactgg ttagagtgaa actttactga caaagtgatt gttgcttcat
     1201 ggagtaaca
//
LOCUS       DASJSZ010000218         1195 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.226, whole genome shotgun sequence.
ACCESSION   DASJSZ010000218 DASJSZ010000000
VERSION     DASJSZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.226"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1076
                     /locus_tag="U4B03_002922"
     CDS             <1..1076
                     /locus_tag="U4B03_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2069700.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DASJSZ010000219         1194 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.227, whole genome shotgun sequence.
ACCESSION   DASJSZ010000219 DASJSZ010000000
VERSION     DASJSZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.227"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..948
                     /locus_tag="U4B03_002923"
     CDS             <1..948
                     /locus_tag="U4B03_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2069701.1"
                     /translation="VRQLLKYEPKAIRIYSRGEEKQFYMQQELSNHTNLRFLIGDIRD
                     SQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMGTQNVIDAAIKNNVKHFVG
                     ISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSCVRFGNVMGSSGSVIPLFS
                     KQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFGETYVLKMPAINILTLAQS
                     LIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSYENDKMYIIPPHYHATD
                     ELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            960..>1194
                     /locus_tag="U4B03_002924"
     CDS             960..>1194
                     /locus_tag="U4B03_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEN2069702.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELS"
BASE COUNT          473 a          157 c          221 g          343 t
ORIGIN      
        1 gttcgtcagc ttttaaaata tgaaccaaaa gctatcagaa tttatagtag aggtgaagaa
       61 aaacaatttt atatgcaaca agagctctca aatcacacta atttgagatt tttaattggt
      121 gatatcagag actctcaaag gattgattat gcatgtcgtg atgtggatat tatttttcat
      181 gctgccgcaa tgaaacacgt tccagcaagt gaatataatc ctatggaagc tgtaaaaaca
      241 aatgtgatgg gaactcaaaa tgtaatagat gcagcgatca aaaataatgt aaaacatttt
      301 gtaggtatca gtacagataa agtagtgagt cctactaaca ccatgggtgc gacaaagtta
      361 ttgagtgaaa aattaataat agctgccaat cagtataaag gaaaaacaga aacaatattt
      421 tcttgtgtaa ggtttggaaa tgtcatgggg tcaagtggat ctgttattcc tctttttagt
      481 aaacaaataa aaaacaataa agatattact ttgacagatg ttgatatgac acgatttatg
      541 atgtcaatac cggatgctgc aagattagtt ctgtctgctg ctatgaattc aaaatttgga
      601 gaaacatatg tcttaaaaat gccggcaatc aatattttaa ctttagctca atcattaata
      661 aaaatatata atgatacaca tgatgaaaaa tatagaggtg aaataaaagt tgtaggtatc
      721 aggccaggag aaaaattata tgaagagcta atgactacag acgaaattga acgctcatat
      781 gaaaatgata aaatgtatat cattccacca cactatcatg ctactgacga actgtacaaa
      841 gattatgaaa aaattaaaat cactgcatac tcttcagatg caacagaatt aatgagtgga
      901 caggaattta ttagaatcgc aaaagagaat aatgtcgtga cttggtaagg agttttatga
      961 tgaaatcaga agtatatgta attgcagaaa ttggtgtaaa ccataatgga agtctagatt
     1021 tagctttaga aagtattgat aaagctaaag aatgtggagc taacgcggtt aaatttcaaa
     1081 catttaaaac gaataagcta gtttccaaaa aggctccaat ggcaaaatat cagaaggata
     1141 atcttaaaga ggacagttct caatttgaaa tgctaaaaaa attagaatta tctc
//
LOCUS       DASJSZ010000220         1176 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.228, whole genome shotgun sequence.
ACCESSION   DASJSZ010000220 DASJSZ010000000
VERSION     DASJSZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.228"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            61..933
                     /locus_tag="U4B03_002925"
     CDS             61..933
                     /locus_tag="U4B03_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2069703.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          344 a          221 c          282 g          329 t
ORIGIN      
        1 aaatatgata tatagtcatt tatttagtga tttcattcta aaataaaggg agaaatgtct
       61 atgctatacg gtgcaattga agcaggcggc acaaaatttg tgtgtgccat tggcgatgaa
      121 gaaatgacga tcaaggaaag agtgagcttt ccgacaacga cacctgagga aacaatgcct
      181 ttagtcattg acttcttcaa acaataccaa gcagatttag cagggattgg gattggctca
      241 tttgggccta tcgatatcca tcgcgattcc gccacttatg ggtacattac ctcgaccccg
      301 aaattggcat ggcaaaattt tgatttcatc ggtaccatga aaaaagaatt tccgatcccg
      361 atttcttgga caacagatgt caatgcggct gcctatggtg agtatgtctt cggcagtggc
      421 aaaggattga gcagtgtcgt ttactatacg atcggtacgg gtgttggcgg cggtgcctta
      481 caagaagggc gctttatcga agggtttagt catccagaaa tggggcatat gctcgtcgtc
      541 cctcatccaa aagatgactt tgctggcagc tgtccatttc atggcaactg tttagaaggg
      601 atggccgctg gacccgccat cgaaaaccgc ttagggaaaa aaggacaaga ggttgcagaa
      661 gatgatcctt attgggaaat cgaagcctcc tatattgctc aatgtgccta caatacaacc
      721 ttgatgtttt ctccagatgt gatcattttt ggcggtggtg tgatgaaaca acgccatatg
      781 ctgcaaaacg tccacgacgc atttgcaaaa ttggtcaatg ggtatgtcga aacacctgag
      841 ttaagcaaat atatcgtgac cccagcatta gaagacaacg ctggaacgct cggttgtttg
      901 gcattggctc gagaagcggt cttacattca taaacgaaaa aaggctcttc gacatttagt
      961 gttggtagag aaagatacaa ctgaaaattt agtcataacc agttaggcag agtgcttaac
     1021 tggtttttgt tttacttgga agatcaagcc ttgaatgaga taaattcttg ctcgaaagtt
     1081 atggaaattt cgatagccgt aggccacttg tattgtgcaa tagtttttta caaaaaaaag
     1141 ggggtgcgga tatttttttg gaccttttta acaaat
//
LOCUS       DASJSZ010000221         1158 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.230, whole genome shotgun sequence.
ACCESSION   DASJSZ010000221 DASJSZ010000000
VERSION     DASJSZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.230"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..283
                     /locus_tag="U4B03_002926"
     CDS             <1..283
                     /locus_tag="U4B03_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2069704.1"
                     /translation="FDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDL
                     ELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
     gene            complement(267..860)
                     /locus_tag="U4B03_002927"
     CDS             complement(267..860)
                     /locus_tag="U4B03_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEN2069705.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
BASE COUNT          352 a          234 c          197 g          375 t
ORIGIN      
        1 ttttgacatc aatcgatcat tgagtcataa aggctgtcct tttgataatg ccgtagctga
       61 atcaacttat aagtcgttga aagtagaatt tgtctatcaa tacacatttg aaaccttaca
      121 acaattggat ttggagttat ttgactatgt aaattggtgg aaccaccttc ggttgcacgg
      181 tacacttggc tacgagacac cggttggtta ccgtaaccag agattggcgc agcgaatcct
      241 tgataatgag ctcggatgtg ctaacgctag cgaggcagtc taactttaac tgttagctcc
      301 tgccgaagat cgtcacatcc gaggaggctc attgtcaagg acaatcggaa tagcatacgg
      361 aaagaagttc tgcaccttat aaaatttgtc aaaaaaactg ttgccattcc accagacact
      421 tgcttaaagc gaaaaatttc ttcaatgtcc atgcctaatt ctttggatat tctcttgtct
      481 gaccatccat tttttactaa tctttggatg acaatttcca ttcctcttat ttcatgggtc
      541 cctcttgcac gattgtgtcg aattgttgaa gccatacgtt cttctatggg tttatcaata
      601 actgttaccg gtacaacttt taagttgagt ctttccttag caacacgata cctatgaagc
      661 acatcaacca agatatactt ctcttctttt tccttgtaat aacaaactaa tggttgagta
      721 aagccatctt caatgataga aagctccaat aagttcattt ccggagacgc tactcgattg
      781 ggattataat cgttcgcctg tattttggta attggaacaa gccgaacgtc catcacaggg
      841 aaagttatgt ctttattcat acgcttgtat accctacttt cttaaaaagt agggtatact
      901 caagaatccg attaattatc ttctcaattt ggataatata tctcatcagt cttcttgtca
      961 ctttatcaac atgattttgc tgcaaaatgg gataccgaca gtactggact ggtaatcagg
     1021 ataaaagcaa aaataattaa tttgtatcct aaaaaatcca taaaaattct ctcacatttt
     1081 cgggtattaa ttcgagaatc acacctataa atttattact atataaggga tacttaaacc
     1141 aaatacccca tctaatta
//
LOCUS       DASJSZ010000222         1149 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.231, whole genome shotgun sequence.
ACCESSION   DASJSZ010000222 DASJSZ010000000
VERSION     DASJSZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.231"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(269..1117)
                     /locus_tag="U4B03_002928"
     CDS             complement(269..1117)
                     /locus_tag="U4B03_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2069706.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          367 a          191 c          197 g          394 t
ORIGIN      
        1 ttttttaatt ttttagaaaa taagaaacaa aaataataaa aaaaagaaaa gataaatttt
       61 ttactactgt atactaactt tgttatcgca tctaacaagt aactcgaata ataccaatac
      121 aaccttctcc aaagatgcga tgacatacgt tctaacccac cgactattga tgttctgtac
      181 cacccttgca gaaccaaaaa agccactacc aaatatttgg tagtggcttt ttttgtttta
      241 tggatttttc gctttttctg ttatggtgct attctttgat ttcagaaata gtatgtcgaa
      301 ctttagcgat agaacgacgt gattcttttt cgttgtaaaa catcaaaatc acatatagag
      361 agtccaaaat gcttagttga gaaatacggg aagctaaagc ttcagatcgg aattctgttt
      421 cttcagaaat agaaataaaa acgacatcgc caagtttagc aagggaagaa ttagcttggc
      481 tagtaataac gattgttttt gctccagttt ttcttacggt ttcagcaata tggattgttt
      541 cttttgattg ccctgtgtga gaaatcaaaa cagcacagtc tttttccgtc aataaagaag
      601 cttccatcaa ttgaatatgg taatccgtgc tgtgagaagt tgcgataggg gaacgcaaaa
      661 atttatgata agcgtcagtt gctacaatct ctgaaccgcc aacaccgaaa aaatagacac
      721 gtttagattc attgataata gtagccgctt tttttagatc cttttgttgt aacaactttt
      781 ttgtgtcatt taaagttgaa atactagact cgaacacctt ttgcgccata gtgagctcag
      841 tatctgtttt ctgaatattt tcgtggatag aaatagcaga tagatcattt tcttggatca
      901 acatggccat tttaaaatct ttaaatccat tataaccaag tttttttgta aattgaaaaa
      961 aagtagagtc agcaatgccc agttcacttg ctaaattgct aataggacta tgacttactt
     1021 tgcttggatt ttctaaaata taatcggcga tagcctgttc ttttgggctg aagtcgttgt
     1081 acattgtttt gatttgtagt cttatggaga cgctcatgag aattcctcat tttcataata
     1141 agattatca
//
LOCUS       DASJSZ010000223         1099 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.232, whole genome shotgun sequence.
ACCESSION   DASJSZ010000223 DASJSZ010000000
VERSION     DASJSZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.232"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(419..922)
                     /locus_tag="U4B03_002929"
     CDS             complement(419..922)
                     /locus_tag="U4B03_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2069707.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
BASE COUNT          436 a          158 c          196 g          309 t
ORIGIN      
        1 tttcctttga aatgccttca aaaagtagga tcaggcagaa tatactggca gaatcaagga
       61 gaagggagcc taaataagga gaaaaaccat agaaaatata aagtaattac ttataaaaca
      121 agctttttat agaaaaataa tatacctgag tagggtataa attaagacgg aattcctttc
      181 tagattgaga tattgactag gaagaatgtt aagattttaa gcctttaagc taaaaaaaaa
      241 ccctttttgt taaataaggg gaatatagcc tgtcagtttc aaactgcaag tcgtcctgct
      301 gcaataatat agtagattag agatgaaaat agattttttt aaaccaatga ctgaataaaa
      361 agaccgtcat agatacacat tggaatgtgt ctattacggt ctttttttac gaacgatttt
      421 attgaagacg gacagttcgg gtcaaaaata aaaaagctac gatcagaaat aagaataaaa
      481 tgtatcccaa aataatggct agacggtcta acttatttgt cgatagatgt ttgtttagaa
      541 gttgataggc ccataaacct atcagtcctc ctgttgtatt agttatcaaa tcagtgatgt
      601 ccgctacgcc aataccgaat aggaattgaa tcaattctgc caagaagctt acaaggaaaa
      661 taatccctaa ttttttagaa aaagaaagtc tcttgaaatt gacattcaaa agcaccccaa
      721 aaggaaaaaa gacaatcaag ttataagtgg tctcacgtaa taccgtcgta ttttcaaaag
      781 aaaaagtaga aaaagggacg agattgacat tgctgtaaga ataagtgaga aattgtggaa
      841 gattaaatga tagcttgaat aaaatcaacc ataatagaaa gaataaataa atccctaata
      901 aaaattttga tacataccgc atagacatct cctccattta aaaagtatac catcgaaaac
      961 cgctgaaaaa tcataaaaaa gtatgaagtt taaggaccaa cgtagttatc gtacgaaaat
     1021 tagaagcttt tttttgttgg gaatccggtt tctttaacaa agctaagata gcgagttgct
     1081 tggaaaagtt gctatcttt
//
LOCUS       DASJSZ010000224         1095 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.233, whole genome shotgun sequence.
ACCESSION   DASJSZ010000224 DASJSZ010000000
VERSION     DASJSZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.233"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            270..>1095
                     /locus_tag="U4B03_002930"
     CDS             270..>1095
                     /locus_tag="U4B03_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN2069708.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKL"
BASE COUNT          386 a          152 c          188 g          369 t
ORIGIN      
        1 ggtagtataa tttaaaggta ataagttatt cttttattac tatgtttggg ttttatagca
       61 aagaaagttt aataagagtt tggacctagc aaacaatatt aatgaggtca ttaaatctta
      121 ttatctttag tgatataacg aaatatctac acagacaaaa tgatagcgta aaatattttg
      181 tgtaaataga tatttagggt agaaaaaaag aaagtccatt ctgtaaaatt aaagttacca
      241 caaccgaaat tttataggag gactttctca tgcccaattt tactacagaa attatggaaa
      301 cactaatcaa taaaggtgat ttagatgaat tatttcgatg tcatttagaa ttagctgtta
      361 attcattatt gcaggctgaa ttaacagctt ttcttgatta tgaaaaatat gatcgtgctg
      421 ggttcaattc aggtaattcc cgtaatggga attattcacg ttcattcaga acagagtatg
      481 gagaattaaa tttggtaatt cctagagata gaaatggtaa gttttctcaa caaactttac
      541 ctgcttataa aagaaccaat gactctttag aaaccactat tattcagcta tttcaaaaag
      601 gaattactat gtctgaaatc tctgaattga ttgaaaaaat gtacgggcat cactatacgc
      661 cacaaaccat ttcaaatatg actaaaatag tttctgaaga tattattgcc tttaaagaaa
      721 ggtccttaga atccagatac tctgtcattt ttatggatgc tactcacatt cctttaaaaa
      781 gacaaactgt ttcaaaagaa gcagtttata ttgtaattgg cattcgatta gatggaacca
      841 aagaggttct tgggtttagt cttgctccaa ctgaatctgc ttatgtttgg aaagagattc
      901 ttcaagacct aaaagaccgt ggtttaaaag aggttttatt agtcgtaact gatggtttaa
      961 gtggcattaa cgatagtatt catagtgttt atccaaatgc tcaatttcaa caatgttgtg
     1021 tccatatatc tagaaatatc gctcataagg ttcgtgttag tgatcgacaa gaaatctgta
     1081 gcgatttcaa attag
//
LOCUS       DASJSZ010000225         1078 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.234, whole genome shotgun sequence.
ACCESSION   DASJSZ010000225 DASJSZ010000000
VERSION     DASJSZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.234"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            67..1077
                     /locus_tag="U4B03_002931"
     CDS             67..1077
                     /locus_tag="U4B03_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2069709.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT          450 a          170 c          191 g          267 t
ORIGIN      
        1 aaggaaatgg gatatggtat agttaatata taaaaataat aactaattac aggagtgcaa
       61 attttaatga aaaaaaaatc aattattttg gcagctacta gtgcgttggc tgttttaact
      121 ctagcagctt gttcaggcga tactaataaa gatatcgcaa cgatgaaagg cggaacgatc
      181 actgtatctg atttctatga tgaagcaaaa cttgaatcat cgaaccaatc cttagtacaa
      241 cggatgatca tttataaagt attcaataac aaatatggcg ataaagtaac cgataagcaa
      301 gtggatgcag aatacgacaa acaagcgaaa tcattaggtg atacttttga atcacaatta
      361 gaagctgctg gatatacgaa agatacttat aaagaatata ttcgtaacaa tttagctttt
      421 gaagctggct tgaaagctca cgtagatatt acagatgacg atttgaaaac tgcttggaaa
      481 tctttccatc cagaagtaga agcacaaatc atcaaattat caagtgaaga tgaagcaaaa
      541 gatgtgaaaa aatcagcgga tgatggagat gatttctcta aattagccaa agacaaatca
      601 actgatacag agacaaaaga agatggcggt aaagttaaat tcgactcaac gacaacaact
      661 attcctgcag aagtaaaaga agcagctttc aaattgaaag acggtgagat ctccgatgtt
      721 attactacaa caaatcctac ttcttatgcg acagaatact acgttgtcaa aatggtgaaa
      781 aaccaaaata aaggcaatga catggataaa tacaaagatc aactaaaaga tatcgctact
      841 gaaactaaac taagtgacaa tgcattcaca actaaagtaa tcggtgaaga attaaaagat
      901 gcaaatgtaa aaatcaaaga tgatgccttt gaaaatgtgt tgagtgcatt cacaacaact
      961 tcatcaagta cgaaagactc aagtgaaact acagcttcaa caaagagttc tgacacaaaa
     1021 tcaactgatt caacaaaaga atcttcaact gaagaaacaa ctgattcatc aaaataaa
//
LOCUS       DASJSZ010000226         1073 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.235, whole genome shotgun sequence.
ACCESSION   DASJSZ010000226 DASJSZ010000000
VERSION     DASJSZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.235"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(344..778)
                     /locus_tag="U4B03_002932"
     CDS             complement(344..778)
                     /locus_tag="U4B03_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069710.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(795..>1073)
                     /locus_tag="U4B03_002933"
     CDS             complement(795..>1073)
                     /locus_tag="U4B03_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069711.1"
                     /translation="TVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIML
                     KSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          371 a          186 c          118 g          398 t
ORIGIN      
        1 aaatattacc ctttcttaga ttaaacttca gagtattcaa ataacggaat aaggagcctt
       61 atcccgcatt actgaatgag actgtgacat aagtaaaaag gtgacttcaa attccttctt
      121 cggaatttga aatcaccttc tttataatta taaagtatcg gtttcacagc atttctgaat
      181 gattcctaga atcttaaacg aatgattgga gttatcaaca ttttttcact cgtttttagc
      241 gaaacaaaaa atgacatttg tcacagcctc attaaattat tgattctttt tctttataat
      301 tgaattgtcc atatttaaag tgtctagtac tctacatgaa taattaattg ctttcactca
      361 aatacttata atctattaac ttgtagttgt ttaacttttc tgaaaaagtt agacccacgt
      421 aataatcttc ccagctttct ctttcaatta actgatccaa tttttgataa tccataaatt
      481 catcatgtat caaaaagaat aaataacttg atattttagc atctagctta atcagattaa
      541 ataatgtttg tttaaaattg ctaccattaa tatgactgat agattctcta gcttcttcac
      601 ataaactttt tactttatta attactactt tgtcatattc atttaacaca ctattattac
      661 cattacttag acgctcagat tctacgaaac aactcaaatc agaaatttcc caaaaaaatt
      721 ttttgttaaa atccaatttc ctcaactcct ccaaactacg taaatgatat agtctcataa
      781 taaaataaaa cctctcagct aatgttgttg aaattgtcca ctaaacctaa ttgatattgc
      841 ttaggagata cacctgcgta ctttttgaat actctcgaaa agtgtgaaga atctgaaaat
      901 cctaaaccta aagaaacttc actgatagta tggtttgatt tcaacattat tttagccgta
      961 gaaatttttc gcttagagat ataattataa atcgtaatat gcatttcatt cttaaagctt
     1021 tctcttaatt tagtttcact tacagaaaaa taattagcta tttcctttac agt
//
LOCUS       DASJSZ010000227         1063 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.236, whole genome shotgun sequence.
ACCESSION   DASJSZ010000227 DASJSZ010000000
VERSION     DASJSZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.236"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..408
                     /locus_tag="U4B03_002934"
     CDS             <1..408
                     /locus_tag="U4B03_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN2069712.1"
                     /translation="MLPVVMISWLLVSVYTSNELLSSLEKIHLPKKIMLSITVVIRFF
                     PTYRSEIRMIKESLKMRNIRLSFFQPLQYLEFLLVPVLIRATMIAEEMTANAITKGIE
                     SPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI"
     gene            419..>1063
                     /locus_tag="U4B03_002935"
     CDS             419..>1063
                     /locus_tag="U4B03_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2069713.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK"
BASE COUNT          368 a          144 c          178 g          373 t
ORIGIN      
        1 atgttgccgg tcgtaatgat ctcatggttg ttggttagtg tctatactag caatgaatta
       61 ttatcctctt tagaaaaaat acatttaccg aaaaaaataa tgttaagtat tacagtagtg
      121 atcagattct ttccgactta tcgttctgaa ataagaatga ttaaagaatc tttaaaaatg
      181 agaaacattc gcttgtcttt ttttcagcct ctgcaatatt tggaatttct gttagttccc
      241 gttcttatac gtgcaactat gattgctgaa gaaatgaccg ctaatgcaat aacaaagggg
      301 atagaatctc ctgtgaaaag aagttcttac tataaaacaa acatgagctt attagactat
      361 atcatcctgt tttccgttac tatttttttc ttaagtattt tgatttgagg tgaaagaatt
      421 gtttgaattt aacaatgttt ctttttcata taaggatgtt aaggaacaag cgcttgttga
      481 ccttaatttt tcaataaaaa aaggcagtct tacattgatt actggttctt ctgggagtgg
      541 gaaatccaca ctattaaagt taatgaatgg acttattcca agactttatg acggaaaatt
      601 ggttggggaa attttatttg aaaataactc gctacaatcc ttaacacaag aaagtatatc
      661 aaaaaatatt ggctacgtaa gtcaagatcc tagaggacaa ttctttacaa caaatacaac
      721 gagtgagtta gtattttcga tggaaaactt tggatttaca aaagagcata tggataaaag
      781 aatcaatgaa ttagctgaat tattagatct ttcaaatata ttagataaaa acattttttc
      841 gctatcaagt ggtgaacggc agaaaatttc tattggatgt agtttatcgc taaactctaa
      901 attaattata ttggatgaac cgtcttcaaa tttggacttc aaagcgacta agaaacttgc
      961 tgacttactt tgtgatttaa aaaataaagg atttactatt gttatagctg aacatcgttt
     1021 ttactatttg aaagaattaa ttgatcaagt tctatttgtt aaa
//
LOCUS       DASJSZ010000228         1051 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.237, whole genome shotgun sequence.
ACCESSION   DASJSZ010000228 DASJSZ010000000
VERSION     DASJSZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.237"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..610
                     /locus_tag="U4B03_002936"
     CDS             <1..610
                     /locus_tag="U4B03_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEN2069714.1"
                     /translation="NLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVG
                     VLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRF
                     VKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKG
                     IYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          365 a          139 c          166 g          381 t
ORIGIN      
        1 caatttagca aatataaatt atagtgacga acttccttta tcccctttac attggctatt
       61 agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc atatatatga
      121 taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat ttaatttaaa
      181 caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc aaataagttt
      241 gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat cctcagtatc
      301 tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt cagagtcaga
      361 aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag gtttgtgtga
      421 tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg ttgacaaagg
      481 aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata tgaataaaga
      541 agacttagat agtcataagt taattagtta tccaattata gaaaaacaac aaattaaata
      601 tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac aaaaaggcaa
      661 agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt aatgttttta
      721 acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag tgtatattat
      781 gctatccttg tttggatttt aggacgttta aagaggcact tttttacata acttaatcca
      841 tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg caccccaaac
      901 cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca ctaagaagca
      961 taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta gagtaattaa
     1021 ttagatgttt attttatagg actgatacta t
//
LOCUS       DASJSZ010000229         1042 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.238, whole genome shotgun sequence.
ACCESSION   DASJSZ010000229 DASJSZ010000000
VERSION     DASJSZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.238"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            87..347
                     /locus_tag="U4B03_002937"
     CDS             87..347
                     /locus_tag="U4B03_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069715.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(709..948)
                     /locus_tag="U4B03_002938"
     CDS             complement(709..948)
                     /locus_tag="U4B03_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069716.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          377 a          147 c          184 g          334 t
ORIGIN      
        1 aatcacaagt gttattgatt ttcataataa tattttaatg ttattatcat tttaaataat
       61 gaaaaaacat taaggatgga tgaaatatga ataacgaaac tatcatttta ggaaaaaaat
      121 atatttgcca acctattggc ttgaaacaaa aagtgattgg agaagtaatc agtaaattag
      181 aaaattgtgt tgtgatctgc gtagacgaat atcatgacgt cgatcgcaat gaaatattag
      241 aaaaatgcgg aaaagtagtg gcaaaatata ctcacgttta tgagttggaa acaacagaat
      301 atctatataa ctatagcaac aaatatgaac atactttagt ttcataaaga tttaaataaa
      361 gtgccttcaa aagcgagaag accaagcttt tgaaggcact ttatttagtt ggcagtgatt
      421 gttcatgatt tttgatttat tcaaaaggtt cgcctagaga atctgtctct gtaacgaggc
      481 ctggtggaga aactcgataa aaacctatac tgccagaact gtcctatttt cggatagaca
      541 aactagtagt tttaaaaaga tcatccgatc caatcatttc aaaaaatgca aaactgatat
      601 caggatcatt tagctcaggc ttgttatcgt aaagcatttg taaaggttgc atttagttag
      661 gagtaaacgc gaaactatca tcattatttg ctgaagaaga tgaagaagct aattcagagt
      721 ttgtttgaaa taaaggagac gtgctgacag tttctttacc ctgttttttg cagaggtaga
      781 aacttttata tcggaagttt ctaccggaga atttttctca tttgtagaaa ttgttgaatc
      841 gatactattc gaaatttgcg tatctgaagg tatgttcttt gatgcagaag tcccacaagc
      901 agtaagtaac aataaatttg ataaactgat aaacagaatt cttttcatta tgtacacact
      961 ccttatagta tcgtttagca tacacctatg ctcagtatta ggcaaaatca atgcaaaact
     1021 atcaagtctt ttatagtatc ta
//
LOCUS       DASJSZ010000230         1039 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.239, whole genome shotgun sequence.
ACCESSION   DASJSZ010000230 DASJSZ010000000
VERSION     DASJSZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.239"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            8..>1039
                     /locus_tag="U4B03_002939"
     CDS             8..>1039
                     /locus_tag="U4B03_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069717.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLK"
BASE COUNT          410 a          149 c          178 g          302 t
ORIGIN      
        1 gaagataatg ggcatcgaat ttagcgaacg gcaaatgaaa atactatact ttttgattga
       61 aaatgagcac acaacttaca aagaattatc agaacaatta tatgtatcta cgaaaacaat
      121 tagcaaagat attttaattt tgaaagaaat aatccaaaaa atgccagatg tacaaatgtt
      181 ggttcaacct aaaaaaggcg tttcgcttat tggaaaaaaa gaaacacttc gaaatttatt
      241 atccagtcat aaaatcaata tcgcagactc tccggaggac cgtctaactt atgtgtttgc
      301 taaattattg aatactaatg attctctaaa gattcaaaat cttgctgacg aattatacgt
      361 gagcaggtcg acggtagaaa gcacaatgaa agatgttaga aaaagactaa acaagcatgg
      421 gatgtcctta gtttcagatc gaaatggcgt gcgcatcaaa gctgatgaaa aaagtaaaag
      481 aaggttgatg tcagaagttg tgaactatta ttggggtggc tttacagcat ctaacagtaa
      541 aacagatctc ttagacttga atatcaattt cattggtgaa gcgaataatt tcttaaataa
      601 ggacatattg aaaaaagtag ccgaattgct taatcgatac atgaataaga cacaattgaa
      661 atttacagat tatgaatttc aaactttagc tatccacctt gctattgcga tagaaaggat
      721 tgaaacaagc aatttcttat cacgtagtaa taattctgaa gaagaaatct caaagaatac
      781 acttatttta atcaaaatga ttgaaaaagc attttcaatt attgtgccaa tagatgaaca
      841 agcttatgtt gacattcaca ttacttcgat tgaaaaaaat gctatgaatc acgacttaga
      901 tcaaacgaag ctttcctatt tagatatggg tgttgagcta caagagttag taagaattaa
      961 attaaatgaa ttttatcctg atgaagaatt gatcaataat ctagtattac atctcaatgc
     1021 agcagttaaa agactcaaa
//
LOCUS       DASJSZ010000231         1037 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.240, whole genome shotgun sequence.
ACCESSION   DASJSZ010000231 DASJSZ010000000
VERSION     DASJSZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.240"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(20..91)
                     /locus_tag="U4B03_002940"
     tRNA            complement(20..91)
                     /locus_tag="U4B03_002940"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="U4B03_002941"
     tRNA            complement(113..200)
                     /locus_tag="U4B03_002941"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="U4B03_002942"
     tRNA            complement(225..298)
                     /locus_tag="U4B03_002942"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="U4B03_002943"
     tRNA            complement(312..382)
                     /locus_tag="U4B03_002943"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="U4B03_002944"
     tRNA            complement(384..459)
                     /locus_tag="U4B03_002944"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="U4B03_002945"
     tRNA            complement(478..551)
                     /locus_tag="U4B03_002945"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="U4B03_002946"
     tRNA            complement(564..653)
                     /locus_tag="U4B03_002946"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="U4B03_002947"
     tRNA            complement(677..750)
                     /locus_tag="U4B03_002947"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="U4B03_002948"
     tRNA            complement(764..837)
                     /locus_tag="U4B03_002948"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="U4B03_002949"
     tRNA            complement(865..937)
                     /locus_tag="U4B03_002949"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="U4B03_002950"
     tRNA            complement(957..1030)
                     /locus_tag="U4B03_002950"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
BASE COUNT          281 a          285 c          246 g          225 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaatt
//
LOCUS       DASJSZ010000232         1022 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.241, whole genome shotgun sequence.
ACCESSION   DASJSZ010000232 DASJSZ010000000
VERSION     DASJSZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.241"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..460
                     /locus_tag="U4B03_002951"
     CDS             <1..460
                     /locus_tag="U4B03_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEN2069718.1"
                     /translation="LLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            469..696
                     /locus_tag="U4B03_002952"
     CDS             469..696
                     /locus_tag="U4B03_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2069719.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          319 a          166 c          181 g          356 t
ORIGIN      
        1 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
       61 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
      121 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
      181 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
      241 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
      301 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
      361 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
      421 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
      481 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
      541 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
      601 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
      661 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
      721 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
      781 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
      841 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
      901 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
      961 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1021 tg
//
LOCUS       DASJSZ010000233         1020 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.242, whole genome shotgun sequence.
ACCESSION   DASJSZ010000233 DASJSZ010000000
VERSION     DASJSZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.242"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..769)
                     /locus_tag="U4B03_002953"
     CDS             complement(<1..769)
                     /locus_tag="U4B03_002953"
                     /inference="COORDINATES: protein motif:HMM:NF026247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HEN2069720.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYE"
     gene            complement(741..>1020)
                     /locus_tag="U4B03_002954"
     CDS             complement(741..>1020)
                     /locus_tag="U4B03_002954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069721.1"
                     /translation="VYFYHHVGDGENLKIGSKKIKIDNHNSTRKYFIARNSTLVKRMY
                     KSDKEFSRLMNKRLLKFFIAIAFETDKLSRFRAAIRGVRDGYRGVIGD"
BASE COUNT          387 a          169 c          136 g          328 t
ORIGIN      
        1 tttcgtaaat attcattttt gataatttat gaactaaaaa aagatttgct gaaaaaaaga
       61 gagcaaacat cactacgtga gaaatcgatg tactgtttct atcaaaatat gcatcatact
      121 tcgtatttat aaatattgaa ctgatataac tttgtccaat agagaatagg agcaagctaa
      181 ggattgtaac atatccagta atctttttat taatttttac aataaaaaaa gggagataaa
      241 aatatgcaag attatggaac agtccagcca ctaagataaa aaaaatatac ttaaatatag
      301 ctttgctgcc tcgcaattta gttagatgat aaaaagctag taatatgaat atcaatgcgc
      361 taaacatacg tacttgtatt gcttgtgaag taaaaccaaa aataaaaaac aagaacaaaa
      421 ctaaaaaaac attagttgtg aataatctga tgacatacaa taaagctgag agtaagacag
      481 tagaaataat caataaaaaa atctgatact gactggaaat aaaggtacat acgtacataa
      541 cagctctgaa tcccggttca atactatctg aactaaagtc cataccaaat tgtgctatcc
      601 cattatacaa gacagaatat gcataataat cagcgttttg tgtgttaaat gccatgatga
      661 tccacattat caaaagtagc accccagaca aaatcttgtt cgatgtactc catttttttt
      721 gtgaaacact cttttcttga ttaatctcca atgacgcctc tgtatccatc tcgaaccccc
      781 cttatagccg ctctaaatct actgagctta tcagtctcaa aagctattgc aataaaaaat
      841 ttcaataatc ttttattcat aaggcgggaa aattccttat cacttttata cattcttttc
      901 accaatgttg aatttcttgc aataaaatac tttcgtgttg aattatgatt gtctatttta
      961 attttttttg atcctatttt taaattttct ccatctccaa cgtgatgata aaaatagact
//
LOCUS       DASJSZ010000234         1016 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.243, whole genome shotgun sequence.
ACCESSION   DASJSZ010000234 DASJSZ010000000
VERSION     DASJSZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.243"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(55..789)
                     /locus_tag="U4B03_002955"
     CDS             complement(55..789)
                     /locus_tag="U4B03_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN2069722.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          360 a          209 c          183 g          264 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgt
//
LOCUS       DASJSZ010000235         1001 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.244, whole genome shotgun sequence.
ACCESSION   DASJSZ010000235 DASJSZ010000000
VERSION     DASJSZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.244"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            370..1001
                     /locus_tag="U4B03_002956"
                     /pseudo
     CDS             370..1001
                     /locus_tag="U4B03_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DASJSZ010000236          999 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.245, whole genome shotgun sequence.
ACCESSION   DASJSZ010000236 DASJSZ010000000
VERSION     DASJSZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.245"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            80..>999
                     /locus_tag="U4B03_002957"
     CDS             80..>999
                     /locus_tag="U4B03_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HEN2069723.1"
                     /translation="MPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLR
                     AYGNQPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEE
                     FELDGAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVN
                     RKNWIFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYD
                     YLFLLVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNI
                     YLSDCLLEKFNKEQIIWKNPPTPDDWEYFG"
BASE COUNT          333 a          146 c          183 g          337 t
ORIGIN      
        1 aaacatatta gttggggaag aaataaacat acggaacgta ttatttgggg tatatttcgg
       61 ggtctttaat ggtttttcta tgccatttag agaaagcgtc atgaatgaag caaagagtat
      121 cgtgcaatat tttgaatatc tgttcaattt aaaactgtct gcaacaaaac gaatcaaatt
      181 caatttgcta accactatta gtttgttgag ggcctatggg aatcaaccaa tcggaaaacg
      241 tttcttttca gatgaatact ttaagagctc agaaggaatg aagatcatca aggatttatc
      301 acatatttct tttcttcata tccctaaaga gcagatcatt gatgagattt cttatatcat
      361 gttgtttttg atcacggaag aagaatttga attagatggc gcatttggac ttgcggaagc
      421 tcattttcat gatatccaca tgattagtca aaaaatgtca gtggaattga ttgatcaatt
      481 accattttcg attgaaatct ctgaagaaga aagactatat atacgaaaag aacttcgtat
      541 gggtatgatg catgtcaatc gaaaaaattg gatattcgac tttgagatga ctagctttac
      601 gtctaaaaag cagctacagt ttttcttcga gacgtatcca atattcagta cagcaatcca
      661 aaaggtcata caagagaata aacgctattt cttgggaaaa gatgaacgtt tcatgattcg
      721 cttattttat gattatctct ttttattggt cagtatttgt ccggtatcat attttgaagc
      781 accgattcat gtctgtattg atttttcgca aggatctgct tataccgatt acattatttc
      841 tcaagtgaaa ggatttaaaa attacaatat tgtgattgag agtcgaatca cttctcgtac
      901 gaatatttat ttatctgatt gtttacttga aaaatttaat aaagaacaga taatttggaa
      961 aaatccaccg actccagatg actgggaata ttttggaaa
//
LOCUS       DASJSZ010000237          988 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.246, whole genome shotgun sequence.
ACCESSION   DASJSZ010000237 DASJSZ010000000
VERSION     DASJSZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.246"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..472)
                     /locus_tag="U4B03_002958"
                     /pseudo
     CDS             complement(1..472)
                     /locus_tag="U4B03_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(602..>988)
                     /locus_tag="U4B03_002959"
     CDS             complement(602..>988)
                     /locus_tag="U4B03_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2069724.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          340 a          150 c          151 g          347 t
ORIGIN      
        1 ctatatgtta gaaggaaaat taagaaaaat agcaataaaa ataagatatt catgtctaaa
       61 aaaacggctt tccaaatgat gaaataccta aatctaactt attaagaaag ttgaaatttt
      121 tatattcagg aaaccattta ttaatttcat gtgcagcacg taatgcattc cctggagttg
      181 tcaaagagaa aattaattct gctaagctta ttattgaaaa tactgataac ttataattat
      241 atcgtccatt gacaatcaaa tataaactaa ctatacttgt tagaacaaaa aaacaggcgt
      301 tgacttgttc ttgattagtt aaaaatatca ataaaggaat acttacccaa tatatttttc
      361 tatttatttc ttctcctctt aagagttgaa aaaacggata aaaagttagt aaactaaatg
      421 ctactggcca ctgatagttc aaggtggttg ctctccaacc ggtttccccc attactgtca
      481 atggtattaa acagtatata gagcaaacca ataataaatt tttaacattt attttctcaa
      541 aaacatactg ggagcgtcaa taattttgtg taaataaatt gtcctcctgc aaaataatta
      601 gttactcagt aaacattgaa actaatgtat cggttacctg ttgaaaacct ttatggcttc
      661 tgtttagaaa tttttgattg tatgtatcaa aaatgctgac tagaaagcgt tctagtgatt
      721 cttcattttg aaactgctct tttctacggc tgtatctttt aatttgctta ttgaaagact
      781 cgattagatt ggttgagtaa atggttctac gaatgctagg tggaaaatca taaaaagtta
      841 ataagtcttg gttttctatg agtgactgcg tcactttagg atagtttttc ttccatttct
      901 caatcatgcc ggataagaag gtattcgctt cttcttttga gttagcttga taaacagcct
      961 taaagtcatc acagatttct tttcggtc
//
LOCUS       DASJSZ010000238          986 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.247, whole genome shotgun sequence.
ACCESSION   DASJSZ010000238 DASJSZ010000000
VERSION     DASJSZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.247"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>986
                     /locus_tag="U4B03_002960"
     CDS             <1..>986
                     /locus_tag="U4B03_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN2069725.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DASJSZ010000239          943 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.248, whole genome shotgun sequence.
ACCESSION   DASJSZ010000239 DASJSZ010000000
VERSION     DASJSZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.248"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>943
                     /locus_tag="U4B03_002961"
     rRNA            <1..>943
                     /locus_tag="U4B03_002961"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DASJSZ010000240          920 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.249, whole genome shotgun sequence.
ACCESSION   DASJSZ010000240 DASJSZ010000000
VERSION     DASJSZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.249"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>920
                     /locus_tag="U4B03_002962"
     CDS             <1..>920
                     /locus_tag="U4B03_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2069726.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQNALHYSYSNGPLECLN"
BASE COUNT          279 a          186 c          183 g          272 t
ORIGIN      
        1 taagtcttgc caaagaacat tcgtagcgga tacctctgtt gctgaaaaac actgtttcat
       61 tagtcaaaaa gttcgttggt ctgtcattgc tcgcttaaaa gagaatactt ccatgacgga
      121 aatcgctcgt cagaaaaata tttccacttc ttcggtctat cgtgtcatga agcgattcta
      181 ccgcccattg aatcctttca aacagacttt gccaaaggtc ttatgctttg atgaattcaa
      241 atcggttcga agtgttgcca gtgcaatgag ttttatcatg atggacggac aaagccatgc
      301 attattggat attgtcgaaa atcgacgctt gccttattta gaacgctatt tttctcggtt
      361 ttcattagcg actcgtgaag ccgttcaatt gattgttatt gatatgtatg cgccttatgt
      421 ttcgttggtg aaaaagcttt tcccgaatgc tcagttgatc atcgatcgtt tccacatcgt
      481 ccaacatatt ggcagaactt tcttgaatca tcgagtgaaa gaaaccacgg tacgacttaa
      541 aggaaattcc cactctcaac gagggttagg gaaaaaattg aagcgctatt ggaaactcct
      601 acaaaaagaa gaagcaaagt taaactatga aaaacgggtt tggcgtgcca gttttaaaga
      661 ttatttgact gaaagtgaga ttgccgatcg actgttagct gcttgtccca acttacgcca
      721 aggctatcag ttataccaag acattcttta tgcggtaaag aaaagagatc agtcactatt
      781 tgaggattgt ttaactagag acattagcgg tctgccagaa acctatacaa ccactttacg
      841 aacgtttaaa aagtttctgc cacagatcca aaatgcgctt cactacagct attcaaacgg
      901 accattggaa tgcttaaaca
//
LOCUS       DASJSZ010000241          867 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.251, whole genome shotgun sequence.
ACCESSION   DASJSZ010000241 DASJSZ010000000
VERSION     DASJSZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.251"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(387..785)
                     /gene="spxA"
                     /locus_tag="U4B03_002963"
     CDS             complement(387..785)
                     /gene="spxA"
                     /locus_tag="U4B03_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN2069727.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          265 a          174 c          124 g          304 t
ORIGIN      
        1 cttcatgacc aattagtaca cggatcgtat tttcattaat atgttccatt tccattttgc
       61 tacacctcac tttgccacaa tgtttaatat agattcattg tagcgaaatc agcttaaatg
      121 taaagaaaaa aaatctcgct gttaagcgaa tttaataaaa ccagcggatt tttcaacctt
      181 tcaagctatc ttatttaact ataacatttt tggcagacga atactatata gccgactttt
      241 cagctaattt tccctctcta tttttcgtgc aatcatcatc ttctactacc tttttataca
      301 tctaatcaag aatacttttt gtaaaaacaa aaaaacttgg aaaaactatc tttgtaatag
      361 tcttttccaa gtttcgtatt atccttctat aatccagcca tcaattgtgc ttgacgtaat
      421 tctaattgtc gtacatcgcg cggtaggaac cgacgaattt catcttcatt aaaaccaact
      481 tgcagacgtt tatcatcaat catgattgga cggcgaagca atccaggatt gttttgcaca
      541 agttccaata attcttgtaa cggcagatca tctaaatcca tgttcagttt ttggaatact
      601 ttggaacgtg tcgagatgat ttcttctgtt ccgtcttctg tcatttgtaa aatcgctttt
      661 aattcagaac tgttcaatgg ttctgaaaaa atattacgtt caacaaatgg aatctgatgt
      721 tcttgcaacc acgcacgagc ctttctgcag gaagtacaac ttggggaagt atataatgtt
      781 aacaacgtgc accactcctt ttcgtgataa gaatcacttt tttgtaacag aagatcataa
      841 taatctccca tctgtttcta ttataca
//
LOCUS       DASJSZ010000242          843 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.252, whole genome shotgun sequence.
ACCESSION   DASJSZ010000242 DASJSZ010000000
VERSION     DASJSZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.252"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>843)
                     /locus_tag="U4B03_002964"
     rRNA            complement(<1..>843)
                     /locus_tag="U4B03_002964"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DASJSZ010000243          838 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.253, whole genome shotgun sequence.
ACCESSION   DASJSZ010000243 DASJSZ010000000
VERSION     DASJSZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.253"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..239)
                     /locus_tag="U4B03_002965"
     CDS             complement(<1..239)
                     /locus_tag="U4B03_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069728.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHN"
     gene            complement(241..>838)
                     /locus_tag="U4B03_002966"
     CDS             complement(241..>838)
                     /locus_tag="U4B03_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEN2069729.1"
                     /translation="GTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSS
                     DNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRA
                     HGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSI
                     FIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          308 a          146 c          102 g          282 t
ORIGIN      
        1 tgattatggt tagttaacaa gttaatttta cgtttaaagg aagaaccgat tttccggtga
       61 ttcacaaaaa caacatcatt aaatagccct aattctttta tacgctcact atatcttcta
      121 gcgttttcaa attcagaata aataaataaa tctccttttg attctcggta aaaattttga
      181 taaacattta atatattaat caaatgtaaa ggagtccaag caatgaaagc tgtcttcata
      241 ttaattctcc ttttttaaat acaagtaaga aatgcttccc aaataatacc agaaaacaat
      301 tgaaatgaca tcgcgcttat tgaaaagtac atgagcctcc accatttcat ctataaaaat
      361 agatagtata aaaattaaaa caataccaag aaacgcataa ttcactttac ttctaaaaaa
      421 tgttttgagt aaactaaaga tatttaacac atagaaagca tagatacata taaaagcaat
      481 tataccactt gatacaagta cttgaagaaa tccattatgc atgtttccat gtgctctttt
      541 tagttcattc acatctgtag tatcaagatc tacttttttc aaaaattttg catcgctatt
      601 cccatagaaa tcggtctctc cataaccaaa taacaaagtt ggtcttataa ctttaagcgc
      661 agctttccaa atcgtaaaac gaccagctga aatttcggag ttatcactag aatgctgtaa
      721 tattacttct ttttctggaa gtggagtttt atctgattca tttgactcta taacttctac
      781 ttttctatca atcgatacag gaattgttgc taaaaaatcc ttagcataat tagtacca
//
LOCUS       DASJSZ010000244          832 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.254, whole genome shotgun sequence.
ACCESSION   DASJSZ010000244 DASJSZ010000000
VERSION     DASJSZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.254"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(40..339)
                     /locus_tag="U4B03_002967"
     CDS             complement(40..339)
                     /locus_tag="U4B03_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069730.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(345..575)
                     /locus_tag="U4B03_002968"
     CDS             complement(345..575)
                     /locus_tag="U4B03_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069731.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
BASE COUNT          289 a          145 c          114 g          284 t
ORIGIN      
        1 ataaaatccg ttccgggcta cctttttagg tagcctactt taatctttgt atctttctgg
       61 atcaacgaaa gtatacttta tatagtcata acgccgatga tcgcttcgag cgtctagcac
      121 atcagtcacg atatcaaaca aaaaatatac gtctttcttc attctagttt tcgcagcagg
      181 gattttaaag tagttcttat tagaatagta gagattaatt aataagctat cttcgattgc
      241 taaaaagaaa acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg
      301 atcaaattta aaccataatt gcgtctcatt aaaaagcata accattactc cgatctattt
      361 ttaaacttag tctctacctc taatatatat cgagttttta ttttgccttc agagaatact
      421 gtttcttttt ttgcagttac aggttgttta ttttcggaaa aagctaatat agctaaaatt
      481 gaaacatcca tctggaattt gtctttttcg ttgctttgct cataaaaatc tgcatattca
      541 tcactaatat tttttctaat aaattcttcc atcataaaga tcacctcgaa aagagtatac
      601 gaacaaacgt tctttttgta aaggtggaat ttattatact aaataaatag gtgaacaagt
      661 acttgtgcca aattatgtgc caaaaaaaat cgaatttaat aaaacacaaa caaaaagaaa
      721 tcctattatg ataacatttc ttataataac aaacacaaca gaagacgtgt aatagttagt
      781 caggaacgta caaataaccc ctgtatcctt tgcggtacag ggggttattt tg
//
LOCUS       DASJSZ010000245          812 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.255, whole genome shotgun sequence.
ACCESSION   DASJSZ010000245 DASJSZ010000000
VERSION     DASJSZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.255"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(195..593)
                     /locus_tag="U4B03_002969"
     CDS             complement(195..593)
                     /locus_tag="U4B03_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN2069732.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(590..>812)
                     /locus_tag="U4B03_002970"
     CDS             complement(590..>812)
                     /locus_tag="U4B03_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEN2069733.1"
                     /translation="KYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDGSVRMI
                     KAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          256 a          143 c          119 g          294 t
ORIGIN      
        1 atcagcccac tagttctaaa ccactatttc ctactattca ctcttttgta ttccttttaa
       61 atcaattatt tcaaaattaa tccatatttt ccatattttt atgaaaaaac tattttgtat
      121 gattaaacat aaaaaaatca gcatattcct taatgacatg ctgatctaat tgagaaataa
      181 agtttttaat tcgttcattt tattgcggtt tgtcgtaaat aactggttat cttggacttt
      241 agtgaatcga aatccaatga actagtggta atcattatga taaaatcaac agcttcttga
      301 cgagaaaaag caactggaca attattcaat tgaagaaaca acaacatggc aacaagagca
      361 gttcgtttat tgccattatg aaaaggatgt tttttagcta aattaattgc taaaatcgcc
      421 gctttttcat agatttcagg atagagttct tgattgaata ctgcttgtga aggctgtttg
      481 acggccatat ctaatgcatt tgcatctttt acaccgatca tttcgcctgg cgagtgacgt
      541 aaaataactt tcgcgttgat tttgataatt tctttagtcg aaagatacct cataaatcaa
      601 ccaaatcttt taacgcgtct tcatattgat tcatgatcga atcaactaat gaatcaatat
      661 caggttcttc ttgtgctttg atcattcgta cagaaccatc agattgaaaa acataactga
      721 ttgcgtcacc tgtctgcaca cccatgaatt cttttacttc tggtgggata gttgttacat
      781 cgctattgcc agattttcta attttatact tt
//
LOCUS       DASJSZ010000246          809 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.256, whole genome shotgun sequence.
ACCESSION   DASJSZ010000246 DASJSZ010000000
VERSION     DASJSZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.256"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            147..533
                     /locus_tag="U4B03_002971"
     CDS             147..533
                     /locus_tag="U4B03_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2069734.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            627..>809
                     /locus_tag="U4B03_002972"
     CDS             627..>809
                     /locus_tag="U4B03_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069735.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEI"
BASE COUNT          292 a          127 c          130 g          260 t
ORIGIN      
        1 aaaacatttt tgaaaataaa catcagcagt cctatcaatg catgtgagct tatagaagtt
       61 attcgcagcg cagcataatt gcattagctt cttttcgtgt tttttgcctc ttttcccttt
      121 aaatcttaat ggaggaggtg tgcaatatgt cagataacga cgaacgcgta aatgcccagt
      181 tgattcgatt ctttgaaaag gtcattttga acaccgctaa tacttattat aaaaaacaag
      241 aagaaattca aaaacatgaa caacttgatc aactcccacc atatttgttt actaaagata
      301 ctataaaatt taaacaaccg attaatgttt taaatgtgac tctccttgtt gaagatataa
      361 aattagcaaa aatccttcct ttgttgaaag aacctgaaca aatttttctt atcgaaaaat
      421 ttatctttga taaaactgat aaagaaattg gaaaggttct ggggattaca agacagggtg
      481 cgacaaattt aaaacatcga ctttacaaaa aaattcaaaa aatgctggtt taatatctgc
      541 ataataacta attttgtatg cttctttgct cgttctcctc cttcccaaag aaatagatta
      601 taagtccatg ggaagaaggt agatttatga attctttaaa agaagatttc attttagcaa
      661 aggctggaaa tgaagaagct gttgaggcaa ttctcaaaag atttagctct ttgattcata
      721 agcaatcctg gagaactgga aaatatgatc aagattgtta tcaagaatgt atgcttgcaa
      781 tatatctagc tatctcgaaa ttcgaaatt
//
LOCUS       DASJSZ010000247          798 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.257, whole genome shotgun sequence.
ACCESSION   DASJSZ010000247 DASJSZ010000000
VERSION     DASJSZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.257"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            252..>798
                     /locus_tag="U4B03_002973"
     CDS             252..>798
                     /locus_tag="U4B03_002973"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN2069736.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSV"
BASE COUNT          288 a           88 c          172 g          250 t
ORIGIN      
        1 aagtaaatgc atttaaagtt agaactctaa atgataagta tgtcgctatt ttcatgattc
       61 cttcagtttt tcagatgata taccactcaa aaaacaatat gagagatgtt caggaatacc
      121 atctctaatg gcttataaat tggttttagg gatagtagat gttatagaca tatcaaggaa
      181 taaacgagca gctgcataaa agcagatgga attaaggaat aattgaattg taaaagaagg
      241 gaataaattt aatggcacaa ttattttaca gatatggaac aatgaatagt ggaaagagta
      301 ttgagctact gaaagtggct cataattatg aagaacaagg gaagagagtt atcataatga
      361 caagcgcaat agatactcga tcaggatttg gaaaagttga gagtcgtata ggtgtttcga
      421 aaccagctat tgcaattact tctgaaatgg atgtgtacgg agaattagtg gagattattg
      481 atcagtacgg aaaagttgat tgtattttgg tcgatgagtc tcaattttta agtagagaga
      541 atattattga tctatcactt atcgtaatga atgaggaagt accagtaatt tgctatggat
      601 tgaagaatga ttttcaaaat aatctcttca agggatccga agcattattg acatttgctg
      661 ataaaattga ggaaattaaa actatttgcc atttttgcaa taaaaaggct gtaatgaatg
      721 ctcgtattgt tgatggtatt ccagtttatt caggtgaaca gattgtagta ggaggtaatg
      781 aagattacct ttcggtat
//
LOCUS       DASJSZ010000248          777 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.258, whole genome shotgun sequence.
ACCESSION   DASJSZ010000248 DASJSZ010000000
VERSION     DASJSZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.258"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            9..647
                     /locus_tag="U4B03_002974"
     CDS             9..647
                     /locus_tag="U4B03_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN2069737.1"
                     /translation="MFSGKQYADNILLWGGEDNIEKINNNLTILDTALENKEQKISIL
                     NSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLENQLSAKDSELQGKQN
                     EINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLNQQINQMLSEKNNI
                     QNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNEHTIQ"
BASE COUNT          357 a          100 c          112 g          208 t
ORIGIN      
        1 tgttagtaat tttctccgga aagcaatatg ctgataatat tttattatgg ggaggggaag
       61 ataatattga aaagatcaac aacaatttaa ctatattaga cacagctcta gaaaataaag
      121 agcaaaaaat tagtatttta aattcacaat tgtcttcaaa caaccaaact cttgaacaac
      181 taagaactaa tattgaagtt tccaaacaaa aagtggcatc tttagaaaac gacaagaatc
      241 aattagtatc agagaaaact aatttagaaa atcaattgtc agcaaaagac tctgaattgc
      301 aaggaaaaca aaatgaaatt aatgcgaaaa tagacgaaat taaccaaaaa gtagctgaaa
      361 taaatcgatt aaatagcgat tggaataatc gtttacaaaa tgaaaagaac aaatttaatg
      421 aagaacaatc tactattaat agtttaaacc agcaaattaa tcaaatgttg agtgaaaaaa
      481 ataacattca aaatcaactg caagctactc agcaaaagct agaagaagaa aagtcaaaaa
      541 atagtagttt gaataattac attgaaaagc ttcaaaaagc taaaaatgat gtagaggata
      601 ccgcaaataa agcacaacaa attgtaaatg aacatacaat ccaatagata tagttttaat
      661 ttaacaaatg gtatgtttgg agataatgag atagaatata aatgtcataa tattatacaa
      721 tctaaacacc gtttcctttt ctatttttga tgacatcagt atatagctac tcgaaag
//
LOCUS       DASJSZ010000249          763 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.259, whole genome shotgun sequence.
ACCESSION   DASJSZ010000249 DASJSZ010000000
VERSION     DASJSZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.259"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>763)
                     /locus_tag="U4B03_002975"
     CDS             complement(<1..>763)
                     /locus_tag="U4B03_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2069738.1"
                     /translation="LTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANLYAEF
                     AHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQE
                     ITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQ
                     LSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLA
                     QIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKR"
BASE COUNT          256 a           85 c          140 g          282 t
ORIGIN      
        1 gacgttttgc tttttccatt tttagttctt taaaatagct aataacactt tttccattca
       61 cttttctaaa atgttttttt aagctggctg gactgataaa aaaaatttgg gctaattgtt
      121 caagcaaaaa attttctgaa agatgttctt ctaaatatcg tatgattgga tcaactaatt
      181 catctgaata aagaatatgc ttttttttag gaatgacttt ctcatttgaa ggtattgtca
      241 ataaagcaag ttcctgttga taatgccttg gtaaggaaga caattgaatt ttttgcagac
      301 aataagtaac taataaggaa agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag
      361 aattgatttc ctgatcatag gaaatagctg gcttactatg ttttagataa tcaattttga
      421 actgttcatt tttcagaaaa gtaatgatag atgttatttc ttgatctata gaaaaagatt
      481 gtcccgtaat atgtgctaat aattgctcat caaaataaaa ctggataatg ataaaagttt
      541 gtgtttcctt ttgagtagaa aagatttgga cttcttgctg tttatcaatg aataaaaggg
      601 aaggattatc tagttctata gatttatgtg caaattcagc atataaatta gcgctttcat
      661 ttattagtag tcgaaaatag ggagcagcag ataaatgaag atgggtatct atggaatagg
      721 ttagtgtttt tttgacaaga ttggaggatt ggttgtttgt taa
//
LOCUS       DASJSZ010000250          696 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.260, whole genome shotgun sequence.
ACCESSION   DASJSZ010000250 DASJSZ010000000
VERSION     DASJSZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.260"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(54..629)
                     /locus_tag="U4B03_002976"
     CDS             complement(54..629)
                     /locus_tag="U4B03_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2069739.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DASJSZ010000251          636 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.261, whole genome shotgun sequence.
ACCESSION   DASJSZ010000251 DASJSZ010000000
VERSION     DASJSZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.261"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            55..>636
                     /locus_tag="U4B03_002977"
     CDS             55..>636
                     /locus_tag="U4B03_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEN2069740.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSF"
BASE COUNT          247 a           82 c          116 g          191 t
ORIGIN      
        1 caccattttc actaatctct aatcaattta ctgaatcgaa aggaaccggg tagaatggat
       61 aagaaagttt taataaagag gttctctgaa gctattgaaa aaggaaatgc agcaatattt
      121 gcaggtgcag gattatcgat gtcacaagga tatgtaagtt ggccggaact acttaatgat
      181 cctgcaacag aaatcgggtt ggattctaaa aaagaaacag acttagttac tctggctcaa
      241 tattataaaa atgaaaatgg tggatcaaga ggaattttga atcagatact tatggataat
      301 tttggtgaag aattagaaat atctgaaaat catcgaatac tagcaagttt acctattgaa
      361 acttattgga caactaatta tgatcatcta atagaaaaat caatccgaga agcttataaa
      421 aaccctcagg ttaagaaaaa ttatactcag ttagcaacaa ctaatccaaa tgtggataca
      481 attgtttata aaatgcatgg tgatatagat gatgtcagtt cgacagtaat aacaagagat
      541 gattatgaaa aatatgatga ttcgtacgcg ttatttaaag aaactttaaa aggagatttg
      601 ttaacaaaaa cttttctctt tttaggattt agtttt
//
LOCUS       DASJSZ010000252          607 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.262, whole genome shotgun sequence.
ACCESSION   DASJSZ010000252 DASJSZ010000000
VERSION     DASJSZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.262"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>607
                     /locus_tag="U4B03_002978"
     CDS             <1..>607
                     /locus_tag="U4B03_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2069741.1"
                     /translation="SGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLD
                     IEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIA
                     NKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTY
                     SIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQ"
BASE COUNT          249 a           49 c          107 g          202 t
ORIGIN      
        1 gtagtggagt atttacaatt aaagaaggta aaaatataga agaaaatgat aagaattcaa
       61 ttattgttca tgaagaattt gctaaacaaa acaatctaaa attaggtgat gaagttgatc
      121 ttgaattact agatattgaa aaaagtggaa aaataaaaag tcataaattt aaaattatag
      181 ggatcttttc tggtaaaaaa caggaaacat atacaggatt atcttctgat tttagtgaaa
      241 atatggtgtt tgtagattat tcaacaagcc aagagatatt aaataattca gaaaataatg
      301 aaattgcaaa taaaatttta atgtattctg gtagtgcaga atctacagac ttagccttaa
      361 acaaattgaa agagcttaaa attgatgagt caaagtattt tgttgaaaaa gattctaatg
      421 cgtttgaaga gtctttagag tcagtgagtg gaataaaata tataataaaa gtaatgactt
      481 attctattat gttaggtggg atggttgttc tttcattaat cttgattcta tggttaaggg
      541 aaagaattta tgaaataggt atattcttat ctattggaac atctaagata caaattataa
      601 tgcaatt
//
LOCUS       DASJSZ010000253          588 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.263, whole genome shotgun sequence.
ACCESSION   DASJSZ010000253 DASJSZ010000000
VERSION     DASJSZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.263"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(65..150)
                     /locus_tag="U4B03_002979"
     tRNA            complement(65..150)
                     /locus_tag="U4B03_002979"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="U4B03_002980"
     tRNA            complement(167..238)
                     /locus_tag="U4B03_002980"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="U4B03_002981"
     tRNA            complement(250..322)
                     /locus_tag="U4B03_002981"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="U4B03_002982"
     tRNA            complement(340..421)
                     /locus_tag="U4B03_002982"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="U4B03_002983"
     tRNA            complement(429..501)
                     /locus_tag="U4B03_002983"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="U4B03_002984"
     tRNA            complement(505..577)
                     /locus_tag="U4B03_002984"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DASJSZ010000254          567 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.264, whole genome shotgun sequence.
ACCESSION   DASJSZ010000254 DASJSZ010000000
VERSION     DASJSZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.264"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          172 a           81 c           78 g          236 t
ORIGIN      
        1 gtatatgaat ttgaaaaaaa ttttttctgt aaaaatattt atagtttatg catttgttag
       61 tttattatat ttatttccag catttaaaaa catggaaata tttggctttt atgatacggc
      121 atttcacatt aatcgtgcgt taagtttaga atctattttt agctccccta ttaattttga
      181 aacgtttaga tcatatggca tgcaagtaaa taatttttac ccatggttaa ctctatatcc
      241 tttattttta ttgatcaagt ttactaattt agcaataggc tataaccttt ttctttatat
      301 tgttacgctc attactctat ttatatgtca ttatgtaatg tatgaaatta ccaaaaaaca
      361 tgttactagc tcctttttcg ctattatata tacaacatct tcttttagga gtgtagaaat
      421 atttcttcga ggggcaatgg gggaattact cgcaatgtca atacttcctc ttattttact
      481 cgggtttata aagctttacg actcaaaaaa agaatcttgg gttatgttgg cgattagcat
      541 gactttatta atttacactc atgttct
//
LOCUS       DASJSZ010000255          535 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.266, whole genome shotgun sequence.
ACCESSION   DASJSZ010000255 DASJSZ010000000
VERSION     DASJSZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.266"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>535)
                     /locus_tag="U4B03_002985"
     CDS             complement(<1..>535)
                     /locus_tag="U4B03_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEN2069742.1"
                     /translation="QNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLI"
BASE COUNT          178 a           91 c           63 g          203 t
ORIGIN      
        1 aaattaaggg agaaagatgc tctatcttag tttttccgat ttccttagta taataaacaa
       61 tcccagaatc cctcactttt aagacctcag gtaacgatac agctaattcg ttttcttgta
      121 tcaaagcatc tctactttga tacgcttcta ttatatccat tcctaatgtt atagacgttt
      181 gtgctaactt ttcaaaaaca atgatagaat aatttttttc agaccgaatc gtatctccac
      241 taacacttgg aattattcca ttactcagat taaaaaccat gttttctaaa tttcgaatat
      301 ctccattttt aacatattcc agtattttat tttcataaag aaacagatat atttctggat
      361 cgtaattttg tgataacaga ttatctattt tgatttgctc gatagtttta cttagatttt
      421 ctgtatattc aattaacggt tcatggaata gatcttctat ttttccagta aaaaaataat
      481 gaactaaaat cagaatatct cgaatgtctc ctaaagaata caaagtcaaa ttttg
//
LOCUS       DASJSZ010000256          529 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.267, whole genome shotgun sequence.
ACCESSION   DASJSZ010000256 DASJSZ010000000
VERSION     DASJSZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.267"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..376
                     /locus_tag="U4B03_002986"
     CDS             2..376
                     /locus_tag="U4B03_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2069743.1"
                     /translation="MQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLADSNEGRYSQ
                     THFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDAEIGKILNMS
                     TSGIWYQRKKAVEQLKMGYDLW"
     gene            370..>529
                     /locus_tag="U4B03_002987"
     CDS             370..>529
                     /locus_tag="U4B03_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069744.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVG"
BASE COUNT          194 a           80 c           98 g          157 t
ORIGIN      
        1 tatacaacac caatttgatg cctactgtaa aaaagtatta cgaaacgaag ctaagtctat
       61 tcgaaaacga aacgctaaca taaaggataa tgaggaacca ttagcagatt caaatgaagg
      121 caggtattcc caaactcatt ttgatgaaca agatgtctat ttcttatttg gaatggaaat
      181 attaatttca gatcaaaagt tatcagcagc aattgatcag ttatcggaga taagaagaaa
      241 gattatccta ctttattatt ttgcaggttt taatgatgct gaaatcggta aaattctcaa
      301 tatgagcaca agtggtatct ggtatcaacg aaagaaggca gttgaacaat tgaaaatggg
      361 gtatgaccta tggtagataa atctaaaaag cggcagtacc tattagttcc actatccatt
      421 atcttatctg ctctagaagg agatccaatt tcgatcaaga tgatactcaa acattatcat
      481 tcttatatgg ctaagttatg tttgacaaat ggatttaacg aggtaggtc
//
LOCUS       DASJSZ010000257          515 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.268, whole genome shotgun sequence.
ACCESSION   DASJSZ010000257 DASJSZ010000000
VERSION     DASJSZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.268"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(38..>515)
                     /locus_tag="U4B03_002988"
     CDS             complement(38..>515)
                     /locus_tag="U4B03_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069745.1"
                     /translation="RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHI
                     PIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNL
                     YEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          158 a           92 c           83 g          182 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctcta
//
LOCUS       DASJSZ010000258          504 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.269, whole genome shotgun sequence.
ACCESSION   DASJSZ010000258 DASJSZ010000000
VERSION     DASJSZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.269"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(116..415)
                     /locus_tag="U4B03_002989"
     CDS             complement(116..415)
                     /locus_tag="U4B03_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2069746.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          154 a           99 c           60 g          191 t
ORIGIN      
        1 acatctgttt ctttatttag aataaaaaaa tacagactct gttcaaataa atgaacctag
       61 cctgcattat gagattgact atacttttta attcattgag atgaataact cgtttttata
      121 aatgcccaaa agtaatcggt gccgaagcca ttcctcgacc taattctttt tcaaattttc
      181 ctattgttga tatctccata taaaacggtg atactggttt agacaattct cccctgattg
      241 ctaacggacg taaactacgt tccaaattat ttatgacttt aggataatac tgaccttttt
      301 ctaagcctgt ctttgcgtct atcaaaagta tacgttcctt ttctttgatc tctggattta
      361 aaacgagatc atataatttt agtaccatgt tttctatttc ttcactttta gacatcacga
      421 tttcctccca aaaatatatt tcttcccact acattctata aacaaagtat acgctacatc
      481 tagatttttt taaatcatta ctgg
//
LOCUS       DASJSZ010000259          499 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.271, whole genome shotgun sequence.
ACCESSION   DASJSZ010000259 DASJSZ010000000
VERSION     DASJSZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.271"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>499
                     /locus_tag="U4B03_002990"
     CDS             <1..>499
                     /locus_tag="U4B03_002990"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2069747.1"
                     /translation="QNKLIIVDNGSDNINSFQFEMMENLILKKMNENIGLATAQNIGI
                     EIAKKEKCDYILLLDQDTIIQKNLSLKLLEEFTNIEAKDKDIGIIVPNYFENSNDESV
                     SFTCKNERGRYVKKHFASTSGSMKIAFANSSGMFFKSELIDDVGNMKDEFFIDQIDVE
                     FCKRVA"
BASE COUNT          189 a           62 c           90 g          158 t
ORIGIN      
        1 acagaacaaa cttattatcg tggataacgg ctcagataat attaactctt ttcaatttga
       61 aatgatggaa aacttaattt taaaaaaaat gaatgagaac attgggcttg cgacagctca
      121 aaacataggt attgaaattg ccaaaaaaga gaaatgtgat tatatccttt tactagatca
      181 agataccatc atacaaaaga atctttcttt aaaattatta gaagagttta ccaatattga
      241 agcgaaagat aaggacattg ggattattgt tcccaattac tttgagaata gcaatgatga
      301 atctgtctct tttacctgca aaaatgagag agggagatac gtaaaaaaac actttgcttc
      361 aactagtggt tccatgaaaa tagcatttgc aaattcctct ggaatgtttt tcaaaagtga
      421 attaattgac gatgttggaa atatgaagga tgaatttttt attgatcaga ttgatgttga
      481 attttgtaaa agggttgca
//
LOCUS       DASJSZ010000260          499 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.272, whole genome shotgun sequence.
ACCESSION   DASJSZ010000260 DASJSZ010000000
VERSION     DASJSZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.272"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..156
                     /locus_tag="U4B03_002991"
     CDS             <1..156
                     /locus_tag="U4B03_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEN2069748.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>499
                     /locus_tag="U4B03_002992"
     CDS             175..>499
                     /locus_tag="U4B03_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2069749.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINRED"
BASE COUNT          222 a           38 c           90 g          149 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagatt
//
LOCUS       DASJSZ010000261          451 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.276, whole genome shotgun sequence.
ACCESSION   DASJSZ010000261 DASJSZ010000000
VERSION     DASJSZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.276"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>451
                     /locus_tag="U4B03_002993"
     CDS             <1..>451
                     /locus_tag="U4B03_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2069750.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DASJSZ010000262          449 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.277, whole genome shotgun sequence.
ACCESSION   DASJSZ010000262 DASJSZ010000000
VERSION     DASJSZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.277"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>449
                     /locus_tag="U4B03_002994"
     CDS             <1..>449
                     /locus_tag="U4B03_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069751.1"
                     /translation="ESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITA
                     DDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKE
                     KVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          182 a           67 c          105 g           95 t
ORIGIN      
        1 gaaagtggaa caacagtctt tgcagtgaaa gacggcgtaa gcttaccaga agataaactt
       61 ccagtgttga aagcaaaaga tggttataca gatgcgaaat ggccagagga agcaactcaa
      121 ccaattacag cagatgatac agaatttgta tcaagtgcaa caaaactgga tgataaatct
      181 gacgctgaca aatataatcc tgaaggtcaa aaagtgacta cagaattgaa taaggaacca
      241 gacgcatttg agggaattaa gaacaaagaa gatttaccaa aagatactaa gtatacttgg
      301 aaagaaaaag tagatgttag cgcagctgga aataaaaaag ggactgttgt agtgacatat
      361 ccagatggat catctgatga agttgaagta gatgttacag tgacagacaa tcgttctgac
      421 gccgataaat atgagccaac agtagaagg
//
LOCUS       DASJSZ010000263          443 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.278, whole genome shotgun sequence.
ACCESSION   DASJSZ010000263 DASJSZ010000000
VERSION     DASJSZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.278"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..218)
                     /locus_tag="U4B03_002995"
     CDS             complement(21..218)
                     /locus_tag="U4B03_002995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069752.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            205..387
                     /locus_tag="U4B03_002996"
                     /pseudo
     CDS             205..387
                     /locus_tag="U4B03_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          161 a           76 c           65 g          141 t
ORIGIN      
        1 aattgattac acgtttttta ttattctgtt aaaggttgag gatcagaaat ttttaggtat
       61 ctataatcct cacctaaaaa tccgttaaaa cgaagtaaaa catcatgacc atttaaatca
      121 gttggaacta aaattggaat taatttatca acagtagttt tgttgtaaag ttcaaaagtg
      181 ttctccattc cttctggttc tacaatcgaa tctttcaata aacaaatcaa gaaatacagc
      241 cgcagaaaag agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt
      301 gatacataca atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat
      361 acattagctt caatgtttac tgagtaaccc attattttgc agaaggacga gttatttaca
      421 caaaattatt gacgctccct gtt
//
LOCUS       DASJSZ010000264          441 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.279, whole genome shotgun sequence.
ACCESSION   DASJSZ010000264 DASJSZ010000000
VERSION     DASJSZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.279"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          186 a           72 c           61 g          122 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac a
//
LOCUS       DASJSZ010000265          437 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.280, whole genome shotgun sequence.
ACCESSION   DASJSZ010000265 DASJSZ010000000
VERSION     DASJSZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.280"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..426
                     /locus_tag="U4B03_002997"
     CDS             <1..426
                     /locus_tag="U4B03_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEN2069753.1"
                     /translation="YSLKKYAGKPIIIVNTATKCGLAPQFKQLEELYQAYQTQGLVIL
                     GFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTDPLFTYLKNEAPGTVT
                     KAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          159 a           85 c           66 g          127 t
ORIGIN      
        1 tattcattga aaaaatatgc aggcaaaccg ataatcattg ttaacactgc aacaaaatgc
       61 ggtctagcac ctcaatttaa acaattagaa gaactttacc aagcttatca gacacaaggt
      121 ctggttatct taggctttcc ttccaatcaa tttaaacaag agctagattc agcgactgaa
      181 gcagcagaag cttgtaaaac cacttatggc gttaccttcc ctatgcatca attgattgac
      241 gtaaatggag aaaatacaga tcctcttttt acttatctta aaaatgaagc tcctggtact
      301 gtcactaaag caataaaatg gaattttact aaatttttga ttgatagaaa tggcaaagta
      361 atcgaacgat ttgcaccaca aacttctcca ttgaaaatga ccgctgcaat tgaatccgtt
      421 ctttaactta ataaaaa
//
LOCUS       DASJSZ010000266          432 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.281, whole genome shotgun sequence.
ACCESSION   DASJSZ010000266 DASJSZ010000000
VERSION     DASJSZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.281"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            190..432
                     /locus_tag="U4B03_002998"
                     /pseudo
     CDS             190..432
                     /locus_tag="U4B03_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT          185 a           38 c           73 g          136 t
ORIGIN      
        1 gagaaaaaat tatccctatt ttaaataata gcaataggta gtggtaggga gaaatttata
       61 aataactttt ttatttgctt tggtagccaa gtaagttgca aaagaaaaat agatactgtt
      121 aaaaagataa tgtagacata gaaacaaata cgtaaaattt tagttagaat aatggcaagg
      181 agaaaaaaca tgatagatga aaaaaagtgt ttaaaaaatc tattagagga tatggatgat
      241 attgaagaaa actatctaaa tggactggga aaacaaatat atttcagata cttaagctat
      301 ctaaaaatga aataagacat agtaattttt tagctttttt gtttgctcca aatgaaaccc
      361 acaatttatc tgatgagttt tttaaaatgt ttttgaaaag gtatatagat tctaatgatg
      421 ataccaaagc tg
//
LOCUS       DASJSZ010000267          417 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.282, whole genome shotgun sequence.
ACCESSION   DASJSZ010000267 DASJSZ010000000
VERSION     DASJSZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.282"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..416
                     /locus_tag="U4B03_002999"
     CDS             <1..416
                     /locus_tag="U4B03_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2069754.1"
                     /translation="TKYYSKDFEIYLNEILANNINPLIEEYANQRHSISKELFFKRAN
                     SKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVEDFFYKITKNQFET
                     EKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT          181 a           56 c           52 g          128 t
ORIGIN      
        1 ttacaaaata ttactcaaaa gattttgaaa tttatctaaa tgagatactt gctaataata
       61 tcaatcctct gattgaagag tacgctaacc aaagacactc tatctctaaa gagctatttt
      121 tcaaaagagc caacagtaaa cttcaagaca agataagaaa tggtgatttc aaatacggtg
      181 aaaaagaaag aatgattgat tctttaaaac agctattaga cgaaatggac ttagcctata
      241 aagatatttc aaagtattca tctgtcgagg atttctttta taaaattact aaaaaccaat
      301 ttgaaacaga aaaatttatt tcagatctta gtaaaaaata caatttccca tatgagaaaa
      361 atagtataat cgcaaaattt ttaactgata atcacgataa atttaaaaat aattgat
//
LOCUS       DASJSZ010000268          351 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.288, whole genome shotgun sequence.
ACCESSION   DASJSZ010000268 DASJSZ010000000
VERSION     DASJSZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.288"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          121 a           57 c           60 g          113 t
ORIGIN      
        1 gtgagaaaaa aggcttataa ttggagtggt agttaattag tgacttattt ttgattttat
       61 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
      121 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
      181 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
      241 cgatgatcgc tacgtgcgtc cggcacgtca gtcatgaatc ggctacacta gactagacag
      301 aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat g
//
LOCUS       DASJSZ010000269          314 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.291, whole genome shotgun sequence.
ACCESSION   DASJSZ010000269 DASJSZ010000000
VERSION     DASJSZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.291"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..309)
                     /locus_tag="U4B03_003000"
     CDS             complement(<1..309)
                     /locus_tag="U4B03_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEN2069755.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     P"
BASE COUNT          109 a           59 c           40 g          106 t
ORIGIN      
        1 aggaataaat tcaaaaggag atacattgtc tttcccttct tgtatttctt tataatcagc
       61 atacccagaa aaatttagtc cagccatact caatcctttt tcatttgtcg catcgtaata
      121 aagagggtag tcagctatac cagcggcaat accaatcatt gcataatgag tatccaaatc
      181 atttaccttt cgaaaattca acttataatt tcttggagta atagtgacta cttcattgta
      241 agatatttca taatcaaaat tccttccaaa ataatgatca cttgttacat aagtaataga
      301 cgtacacata gttt
//
LOCUS       DASJSZ010000270          309 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.292, whole genome shotgun sequence.
ACCESSION   DASJSZ010000270 DASJSZ010000000
VERSION     DASJSZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.292"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..309
                     /locus_tag="U4B03_003001"
                     /pseudo
     CDS             1..309
                     /locus_tag="U4B03_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DASJSZ010000271          309 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.293, whole genome shotgun sequence.
ACCESSION   DASJSZ010000271 DASJSZ010000000
VERSION     DASJSZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.293"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            117..>309
                     /locus_tag="U4B03_003002"
     CDS             117..>309
                     /locus_tag="U4B03_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2069756.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINS"
BASE COUNT          117 a           30 c           65 g           97 t
ORIGIN      
        1 cagaaaagtt atttaatagc ggcttatgct taccttctgg aagtaatatg acagatagtg
       61 aacaagaatt tgttattgat gagtttaaaa aaatattcaa gtaggtgtga tattttatga
      121 acataggaat tattggttta ggttccatgg ggaaaagaag acttagactc attcaagaac
      181 actttaatga tattttacta tgtggtgttg atactaatga ggcgagacga aaggaagttg
      241 aaaaggaatt tggaatacca acttataata gtctaacaga ggcaaaaaat ttgtttgaaa
      301 taaattcag
//
LOCUS       DASJSZ010000272          299 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.295, whole genome shotgun sequence.
ACCESSION   DASJSZ010000272 DASJSZ010000000
VERSION     DASJSZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.295"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          103 a           34 c           55 g          107 t
ORIGIN      
        1 tgaacaaaca taatagaaaa ataacgaata acaaagaact gatcttgtta ctaataggga
       61 tttggggtat ctttttactc tttaatatgt atatgccgac tatgagagcg gatgacgtag
      121 tttatgcaag tagattagat gaacttggct atttgggtgc ttcgatagaa cattataaga
      181 catggagttc aagaattatt atagaactat tcttaatgtt cttttctaaa cactttatgc
      241 tatggaaatt attaaattct acgattatgt taggaattgt agtattactt tgcaagtat
//
LOCUS       DASJSZ010000273          282 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.denovo.296, whole genome shotgun sequence.
ACCESSION   DASJSZ010000273 DASJSZ010000000
VERSION     DASJSZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.denovo.296"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>282
                     /locus_tag="U4B03_003003"
     CDS             <1..>282
                     /locus_tag="U4B03_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEN2069757.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DASJSZ010000274        17214 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.guided.058, whole genome shotgun sequence.
ACCESSION   DASJSZ010000274 DASJSZ010000000
VERSION     DASJSZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.guided.058"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..507)
                     /locus_tag="U4B03_003004"
     CDS             complement(<1..507)
                     /locus_tag="U4B03_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEN2069758.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAE"
     gene            complement(605..1633)
                     /gene="rfbB"
                     /locus_tag="U4B03_003005"
     CDS             complement(605..1633)
                     /gene="rfbB"
                     /locus_tag="U4B03_003005"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN2069759.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(1655..2227)
                     /gene="rfbC"
                     /locus_tag="U4B03_003006"
     CDS             complement(1655..2227)
                     /gene="rfbC"
                     /locus_tag="U4B03_003006"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN2069760.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(2241..3107)
                     /gene="rfbA"
                     /locus_tag="U4B03_003007"
     CDS             complement(2241..3107)
                     /gene="rfbA"
                     /locus_tag="U4B03_003007"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN2069761.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(3207..3941)
                     /locus_tag="U4B03_003008"
     CDS             complement(3207..3941)
                     /locus_tag="U4B03_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2069762.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            complement(3919..4746)
                     /locus_tag="U4B03_003009"
     CDS             complement(3919..4746)
                     /locus_tag="U4B03_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2069763.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(4746..5546)
                     /locus_tag="U4B03_003010"
     CDS             complement(4746..5546)
                     /locus_tag="U4B03_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2069764.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(5539..6678)
                     /locus_tag="U4B03_003011"
     CDS             complement(5539..6678)
                     /locus_tag="U4B03_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEN2069765.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(6883..8076)
                     /locus_tag="U4B03_003012"
     CDS             complement(6883..8076)
                     /locus_tag="U4B03_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2069766.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(8155..8361)
                     /locus_tag="U4B03_003013"
     CDS             complement(8155..8361)
                     /locus_tag="U4B03_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN2069767.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(8354..9496)
                     /locus_tag="U4B03_003014"
     CDS             complement(8354..9496)
                     /locus_tag="U4B03_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN2069768.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10008..10643
                     /locus_tag="U4B03_003015"
     CDS             10008..10643
                     /locus_tag="U4B03_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2069769.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            10985..11620
                     /locus_tag="U4B03_003016"
     CDS             10985..11620
                     /locus_tag="U4B03_003016"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2069770.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            11727..12965
                     /locus_tag="U4B03_003017"
     CDS             11727..12965
                     /locus_tag="U4B03_003017"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2069771.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            13192..13611
                     /locus_tag="U4B03_003018"
     CDS             13192..13611
                     /locus_tag="U4B03_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2069772.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            13616..13855
                     /locus_tag="U4B03_003019"
     CDS             13616..13855
                     /locus_tag="U4B03_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069773.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(14080..15003)
                     /locus_tag="U4B03_003020"
     CDS             complement(14080..15003)
                     /locus_tag="U4B03_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN2069774.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(15035..15799)
                     /gene="map"
                     /locus_tag="U4B03_003021"
     CDS             complement(15035..15799)
                     /gene="map"
                     /locus_tag="U4B03_003021"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN2069775.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            15957..16397
                     /locus_tag="U4B03_003022"
     CDS             15957..16397
                     /locus_tag="U4B03_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN2069776.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(16575..17144)
                     /locus_tag="U4B03_003023"
     CDS             complement(16575..17144)
                     /locus_tag="U4B03_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2069777.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
BASE COUNT         5177 a         3362 c         3064 g         5611 t
ORIGIN      
        1 ttccgctagg cgttgcatcg tgaagacgaa attgtgtcca tagatcccga aaacccaaga
       61 agttcgtatg atataatagt cttctaaaat ctcttctact gctttttcgc ctaataattt
      121 cgtacgacca tattcattta gtggattcgt ttggtcgtct acttcgtatt ctacattttt
      181 gttttttcca tcgaaaacat agtctgtact gatatagacg aattgagcat tcacagcttt
      241 tgctgcaagt gctacatttc ttgtaccatc aacattgatc ttctcatcta attcttttgc
      301 ttcttcttct gctttatcta ctgctgtgta agctgcacaa tggaaaataa catcaggttt
      361 taattctgtg atgtaagcca ttgttttctc ggcatctgtg atatccaagt cttttgcatc
      421 tgttgaaaca tattgcagat ttttttcatc taataaatgg cgcaattctg tacctagttg
      481 cccatttcct ccagttaata aaaacatatg attatccgct ccttgatttt tattttaaaa
      541 aagagacagg cgagcgcctg cctcacatgt gtttttccat taatacagtg acattttctc
      601 tgtattattg tccatttttg gcatatgctg cttcaacggc ctctttttct tttttccacc
      661 agtcttcatg ttcttcgtac catttgatcg tgtcagccaa cccatcacgg aagttcgtaa
      721 actcaggctt ccatcctaac tcttcccgta attttgtcga atcgattgca taacgcaagt
      781 cgtgtcctgc acggtcgttt acatggtcgt acgcatcttt tggttgaccc atcatttcta
      841 agatcaattc catcacggtc ttattatctt cttcaccgtc tgcaccgatc agataggttt
      901 cgccaattct gcctttcgtc aaaatcagcc acactgctga agaatgatca ttcgtatgga
      961 tccagtcacg aacattcttt ccttcgccat ataattttgg acgaataccg cttaagatat
     1021 tcgttacttg gcgaggaata aatttttcaa tatgctgata aggaccatag ttgtttgaac
     1081 agtttgaaat ggttgcacgt aagccaaaag aacgcaccca tgcttttact aacagatctg
     1141 aaccagcttt tgtagaagaa tatggacttg aaggattata tggtgtttcc gcagtgaatt
     1201 tttctccttc gccttcccca tgtcctggca ggtcttcacg taatggaaga tcgccgtaaa
     1261 cctcgtcagt agatacgtga tgatagcgta catcatactt gcggcaagct tctaaaagtg
     1321 tatacgtacc aatgatattc gtttgtacaa atgggaaagg atcttttaat gaattatcgt
     1381 tatgggattc tgccgcgtaa tgaacaactg cgtccgcttc ttttactaga cggtccacta
     1441 attctgcatc tgcaacatct cctacgacca attctacgcg atcgctagga agtccagcca
     1501 agttctcttt gtttcctgca taagtcagtt tatccaaaac agtgacatgc acttctggat
     1561 gattgtttac cacgtaatga acgaaattag agccgatgaa tccggcacct ccggtaacaa
     1621 tgatgttttt cataagaaaa tttctcctta attcttagat ttctccgtaa acaaatggat
     1681 tttctgcttc aaattctttt aatgtcggat gacggtgatc tttttccgac ataatcgctc
     1741 ttgatggatc aattggccaa tcaatcgcta aatctggatc atcaaatgcg atgcccccat
     1801 ctgcttctgc attatagtaa ttatcacact tataaaggaa gttcacattt ggtgtcaaag
     1861 taacgaatcc atgggcatat cctttaggta caagcaactg tcgatgatta tgttcagata
     1921 agataaatcc gtcccattga ccataagtcg ggctaccttt gcggatatcc actactacat
     1981 ctaaaactgc tccagtgact acccgaatca attttgtctg tgcagcttct cctttttgga
     2041 aatgaagccc ccgaaggaca ccagcttcag ctgatagtga atggttgtct tgaacaaagt
     2101 catatttcaa tccatgttct gcaaattttg cttttgaata actttctgtg aagaaaccgc
     2161 gatgatcgcc aaagacgtcc atttctacga ttttgacatc tgttaatttt gtatctgtta
     2221 ctttcatctg taaggactcc ttattcagcg gccaaattca ataaatattg accgtattga
     2281 ttttttttca atggctgtgc aagggccagc aattgttcct tatcgatgta gcccatacgg
     2341 taagcaattt cttccaaaca agccactttt aagttctgac gtttttcaat tgtttcgata
     2401 aaggtagatg cttcaagcaa tgattcatgt gttcctgtat caagccaagc aaatccgcgt
     2461 cccataagtt ctacggatag ttttccttta tccaaatatg ctttattgac atccgtaatc
     2521 tctagttctc cacgttctga aggtttgata cctttagcga tagaaattac ctcattgtca
     2581 taaaaataaa ggccagttac agcgtaattt gattttggct gagcaggctt ttcttcaatc
     2641 gacaaagctt gcatattctc atcaaattct actacaccaa agcgttctgg atcattgaca
     2701 tgatagccaa atactgtcgc gcctgtatct tttgaagctg ctcgttgcaa cattttagac
     2761 agaccgccac catagtaaat attgtcccct aataccaagc agacactatc atctccaata
     2821 aattcttctc caatgataaa cgcttgtgcc aatccatctg gactttcttg tactgcatat
     2881 tcgatctgca gtcctaaatc atttccgttt ccaaataatt ccttgaaacg tggtgtatct
     2941 tgtggcgttg aaataatcag aatttctttg attcccgcca acatcagagt agacattgga
     3001 taataaatca ttggtttgtc ataaatcggc atcaattgtt tcgatgttgc ctttgttaaa
     3061 gggtacagac gtgttccgct gcccccagca agaatgattc ccttcatgga tatctgctct
     3121 cctttttgat ttcaaatttc actttgttat tatctcatca attgttatat ttgtcatgga
     3181 aatattaagg atttaaacat cttttatcaa cgtttaaacc attttcgttt gatcaacgcc
     3241 gtaatcagat gaacacgcca agaaattttt tgccgtaatt tagaatgtgt ttcaatctca
     3301 ctcgcgttta aatcatgtct tcggtacaga gttaacggtt ctcttaggaa cccagtcttc
     3361 tttcctccca gcaaaccaat ccacatatca tgcatcggca cttctgacgg gatcggcaaa
     3421 atatcgggtt ttaaagaaga ccggaaagcc ataccagcac caatatagtg acttcttatc
     3481 acattattaa gccatccttc tttggaattt cgaaacgaaa aatacgaatc atggatctct
     3541 tgcagctcgc tgtccacaat gattaaatca gaaactacta ctagctgatc cggattctgc
     3601 tcaaaatagt cagtgatctt tttcactttt gtctccaacc aaacatcatc ttggtctgcc
     3661 aaaaagatta gatctccttt cgtctgctgg atcgcataac caaaattagc gatcaatcct
     3721 tgacgaggcc ccgaaaaaag ctggattcgc ttatcttttt ctgcgtattc agttaatata
     3781 tcccatgtat catctactga cccatcatca gaaatgacta cttcatcttc tgctgaaagc
     3841 tgagataaaa tactatctaa ctgctctctt aaatatctgc ctccattaaa ggtagctata
     3901 caaaccgaac gcacgaattc acaccttcca attcttaaca tatgagcgca aagtcatccg
     3961 ccagatatgc cggtttttga cggtcaagaa ctgtttagct gttcttaaca ataattgtcg
     4021 tcggtgactg gataaatgcc tttgatcata ttcatgataa aatcgatttt ctgcttgtaa
     4081 aatagaacgg tatcgattac ttgaaacttt ttcatagtct aaaaccgaaa gatcatgttc
     4141 cattcgtaca ggaaggatct ccacagtttt ttccaactga tccactttcc aaaacagcca
     4201 atgatccaaa aaatctaaag aaaaagcagg attaaacccg ccaatctttt ttagaaattt
     4261 gacagaccag gcaacacctg aattgattgc catgattcgt tcagctgttg ggccttcaac
     4321 ttctactgct tgagctgtac gattgatata acgatctgaa tataggggag aaacttgtcg
     4381 gctcccagaa aaaatcatcg gaactgcagc aacacattca tctgtaaagg ttactttacg
     4441 caatagttca aaataatcgt cgactagcct cgtatcctga tccagagtga cgagacaacg
     4501 tgttccttca tctgcttgag acaacgcaaa attataagcc accgctaaac caggattttg
     4561 cggatcatga tgataaacga tattttcttt ttcaaacagc tcattttgct gcctttgagg
     4621 actattatcg taaataataa gctgaatttg cttttcgcta agtgctttgc ttaatggtcc
     4681 ataagctgga gattgactga atagttgctg ataaagcaca acgactacta atatttctgg
     4741 tttcatcaga aaaaacgcca cccccactta ttgaaaaatt tcatcataga agtcaaaaac
     4801 acgcgaaaca gttttgcatt tcgatgcgca cccttcccgt aaaaatggat aactgcataa
     4861 aagggtgtgt acaataactt gtaacctgct ttttcaaaac tcaaacacaa gtcgttgtct
     4921 tcaaagtaca tgaagaaacg ctcatcaaac ccctttatct ctttaaattt atcaatatca
     4981 gctaccataa aactacctga gatcatccgt acataactat tagcatcagt aggcaatcct
     5041 cggcattcaa acacagccag tcgtttgtca aacttttttt tgacccattt gaaaggcaca
     5101 aagcgcaaaa cgtaatcaaa aacagccagc tgctcacgga tgaggtgctg agttgttcca
     5161 tcttcattaa gaatcttagg tgctaacatc gcacattccg gatgttcttt caataacaag
     5221 accaatgctg taactgtctc ttcatccact aaaatatctg ggttacaaat aatgccatag
     5281 cgagaatcgc tagctatcag attgtgatta tgtccataac caaatccgga attttcatca
     5341 tgaaaataga tatcgatgta gggatatttc ctatactctg ttagtctttt tttgtactcg
     5401 ggaagtgaat tgttgtcaaa aattcggatt tttactgctg tttcatcttt tacagttgaa
     5461 ataaaattat ctagtgtccg aaaaatttta tcactgtttg ccgtcacaat actaataagg
     5521 atcggctggt ttaatttgtt attcatctat atgacggttc catttcttca aatatttctg
     5581 ttttacttgc gtacggtact ctttattccc taaaaatcgc aatgttttta aaataaactg
     5641 atgtttctca ttgatcagac caatcaattc aaataataaa attgcagcaa acaacatggc
     5701 aataatcaat atgatcgttc cgattcttcc agtataattc atgaccatag ctgtgaaaga
     5761 aaacatcaaa gccatcgcat aaatcgtcag gacagcacct ttatgagtaa agcctaaact
     5821 gattagtcgg tgatgaagat gttttttatc tgctgtagaa aaagaaacac ggtttgcttt
     5881 tctcctaatg atagcaaaaa acgtgtctgt taccggcact cccatgacga tcagcaaaga
     5941 aatcgatgaa acaaaagtca cgttcttcag cccttgtagt gaaaggacgg ccatcataaa
     6001 ccctaggaat agagctcctg tatcgcctaa ataaatcttt gccggataaa aattgtaagg
     6061 aaaaaaaccg ataatacttg ctacaagaca gaaaataaca actggaatat acactgtaga
     6121 agcatggagg aaaaaatatc caatgattcc tatcgtaatt aaaccgatca atgaaatccc
     6181 tgccgccagc ccatccaaac cgtcaatcag attgaccgca ttcgtgatac cgaagatcca
     6241 aaaaatcgtt acgggaaaac taagccagcc tagttggata tatccaataa aaggaagcgt
     6301 gaaggaatca atacgtatat ctgctacaaa ataaataata agagaagcaa tcaagatccc
     6361 tatacttttt tgtttaggtg tcagctcata tttatcatcg agcactcctg tgactatgat
     6421 tactaaagaa gcaatcaaga tcggtacaat atacgattga gggataattt tacggaataa
     6481 gaataaagtc gatataacaa atgaaataaa gataggcagt ccgcccgctg aaggcattgg
     6541 aactttattt attcttcttt catttggttt gtcgaccata cctgtttgta ctgaaatgat
     6601 tttgaataca ggtgttaata tcagtgataa taaaattgtc agaaaaaatc ttataaccat
     6661 actaaaagaa acaagcatat gggcacctac tttaattaag agtcataatt attataaatt
     6721 atatatgtct ttttgactat ctttttatat cttctcaaat gtccgcaaag ccttatttta
     6781 tcggctctac gggcattttg cttttgtggt aaacctcgca tatctaggtc tatcttctta
     6841 tattttccct gtcaatcgtg gttaaaatcg tggtaaatac ttctatgcga ttagacgctg
     6901 aacctctgat tttgcggtat ctatggacgc atgagcgtac cagttcattg tgatactgat
     6961 gtttgaatgc cccatgatat actgtaaatc tttcgggttc atgttcttgc ttgccagcct
     7021 tgtgcagaat gtatggcgta gcgtatgcgg tgtaatatgt ggcaagggat tgtccttgtg
     7081 gtgcttgttg tatttcttta ccatacggat aaataaggcg ttataatcaa tggcaacttt
     7141 tggattacct ttcggattga taaacacaaa accgctgtgt ccgtctacct ctatcgactt
     7201 tatctttggt cggctgttga taacccgttg aaatgcctgt atcgtttctt tgcttaatgg
     7261 cacttgcctt atcccgcttt tcgtcttagg cgtttcaata taatagccct gttccttact
     7321 ctttagtaac tggtggtcga taatcacaac ttcattcttg aaatcaatgt cggctactgt
     7381 cagtccgcac agttccgaga tacgaagtcc tgtctttaac agtatcagca catcatcata
     7441 atgcttgtga tacacgttgt ccgtcttaat aaatgacaat aaggcttgct cctgttcctc
     7501 tgtcaatgcg actttctctt tggtatcatt ttctatgact tcacttagct tgaaatcaaa
     7561 agggtttttc ctcacataat catcttgtat ggctatgtag aatgacgctt ttaacgagcg
     7621 tttatggttg ttaatggtat tgtaggaaaa gcctttgtct ttcatgcgta acgcccattc
     7681 tttagcgtca gagggtttta tcgtatcaat gctcctagca cctaacttgt cctcttttaa
     7741 taaccgcatg agttgttccc gttgtttctg tgtgcttttc ttcacatttg tcctctgtgc
     7801 gttctgtttg gcatagagtt ggcaaagcgt cattttcttg cctgtgctgt cgataccgtc
     7861 ctcaatatcc cgtcttatct gctgttccag ttctcgaagt gagggttttt cccgttttcc
     7921 ctttggtgtc gggtctgtgg gtgtcaatct ccaagcatac acatattttg tgtttcccaa
     7981 tgcgtctaca tatttatata agtattttcc gtctgttcgt tggctctctc cagtatgcag
     8041 gatacgacct ttgttatccc gtcttttttc tttcatggtg tctgctcctt tccttgttgg
     8101 aaagagcctt gatatgactt gtgttcatca taacacatac aaggctcatt tgcattagat
     8161 tgcgtccaat ttatcaataa ccttttcaaa ctgtcggcgt ttaatctgta tgcggttgcc
     8221 attcataatg agccagccag cgtccttgtt ttcctctgcc aatcgtctta acttgttttc
     8281 tccaatgcgg aaatactttg acgcttcttc aatggtaagg gtgtattttt cccatacggg
     8341 aatatcctgt gtattactca taaaacaccc cctcactttc gattacgtct gatatgtccg
     8401 tacacatctg ttccacaagc atttcctgtt tttcagaaag gtgggtacat tcatcaatca
     8461 ttttcatgta gtttgaacct gtcagcctgt cgatttcctc tatcatcacg attgacggca
     8521 tgacctgtct ttccagccat tgcagagtgc gttccatatt gacaggtaca gggttcattt
     8581 caaaaggtaa actgtcatgg gaaatgaata tctcccacaa tggatagtca ggaaattcca
     8641 caaaatcggt aatgagataa aacaccagcc catgagggtt atacgtcaat aacagttctt
     8701 caactgcctg taccgccttt ttgtcacgca gacgaatttc aaaacggttg ataatatcgg
     8761 tatgtttctt tttcgttgcc tgttcctttt gtttttcata caggcagaaa tattttgtgc
     8821 ttgcctttga gccgatataa agggtacttg ctaaattacg gttttttccg cttaattttc
     8881 caccttgtac attttcatag tttttacagc gacattccgc accaccattt ctgtattttt
     8941 cagatagcac aggaatatcc agcagaccgc acatatcatt gattgccaag tcaaaacgtc
     9001 ggataacacc gccacagtca agacagcggt ttaagaatga ataccagtcc ctgttctgtg
     9061 cctgtaagac atattccatg ttgcgtgagc ctgccccttt taattctaaa aatacgccca
     9121 tgtgttcatc atcagaagcc atgaccttga tttccccgta agcataatgc tccttatagc
     9181 cataataccc atagtcataa tggataaaat aatctgcttt catgccaagc acctttcgga
     9241 taacttccaa tgcgtcagtc gttggaaaac ggacagacag ataatcaacg agcagataaa
     9301 aaggcttatc gcagatatag taattcagac aacgcaggat aatgttctgt gtttccttgc
     9361 tggcgttagc ttttccgttc tcaaagcggt tgatactata tcttgataca tgggtaagac
     9421 ttgcaagttc tccttgtgaa agaccgctgg caagacgttt gttcttcatt tcaagtgcaa
     9481 attctaaatt gttcattgta ttcctccaat cccacaatag ccttgaaata cctgtaaacc
     9541 acctttcaaa aagtgtgata tgtactatct tcttaaaacc tctcattact actcacaggt
     9601 ttgggggtgt atggtttttg acttgtcttt tgtgaaatag tgcccccctg ttagataacg
     9661 ggggcacaga caaggctcgc aggctcgcca gccgacaaag catttcttcc ccttgacaca
     9721 aggtgtcatg gcggtaagcc tgagaggctt ccgccaccgt gtcagaaata ctttgtcagc
     9781 aagttctgtt ttgccttttc taacaagttc catttcaacc gatattcata tctacggatt
     9841 taatcagata ggtatttctt tttgaatgtt tttatatttc attctattgt gcttattcga
     9901 ttacaagaac atagtaattc ttatatgctt atttgtcaat atcttttttt gcctttacaa
     9961 atcatattaa gcatgataga cttataatag cgaagatata acgagatatg gagggttatg
    10021 caatgaaaat aagccattta ggaaataata tacagaccat aagaaaattt aggggaatga
    10081 aacaacagga acttgcggac aaaatcggta tcaatatgca gagcctttcc aagattgaaa
    10141 gagggttgaa ctatcctgcc tatgaaacgc tggaaaagat aatggaagtg ctggacgtta
    10201 cgcccaatga attgttgtcg ggagaatgga agtatgtcaa tcaatctgaa aaggaagtct
    10261 gtcagttttt aagagccgaa gaacgtctaa atgcagaact gaaacatgga cactatgata
    10321 acttctttga cagcgaggaa gaatggctgg aatatgagtt agaaaagtta cgggaatata
    10381 ttacagacta tatcaacggg aaaagcattg aagcgtccga cctttaccca atcaaagaat
    10441 ttatccagca tttgaagttt caaaaattat tagaccgcta tgatgattta tacagtatgg
    10501 atatgtttgg ggaaagcata gagggacata agtataagac accttatcaa gtcgtgaaaa
    10561 tgataaaccc gaactcaaaa gaagatatgg aactgttacg agaagtatta aggaataaca
    10621 attttgatga tgaggacgag taactttttt cctcttgcga gaagatacag caaacaaaaa
    10681 gcccgatcaa gagtgcaaag caccaccaat ggtggcaaag ctcttgacag ggctttttct
    10741 tttctgttgt gggcaatcaa gaggaagaaa gggataaaat ctgatttgtg gtaaatcagg
    10801 caaaggcaac gcaggtatca cagtatgaat tgacaccgca taaggaaagc aactggaaga
    10861 ttgtagaata aatgaaaaag gcttgcattc tgcattttta taaaattgca agtctttata
    10921 ttgacaagca taaaatacta agatatactt taatcagacc aacggtttgt attggaggta
    10981 agttatggca cgaaataaat atccagaaga aacagtaaaa ctcattttag atgtttctac
    11041 aaaaatgttt tgtgaaaaag gatatgataa cacatcattg caagatatta tcaatgaaac
    11101 aaaactttct aaaggtgcta tttatcatca tttcgattca aaggaagata ttttaaaagc
    11161 aatttttcaa agaattggaa atgaaaatgc agtaatattt gctaaaatta gagataacga
    11221 tagtttaaac ggtttagaaa agttaagaga aatatttaag acagcggtat tcaattctaa
    11281 ccaaagcgtt ttattgacag tttctcctca actattaaac aatcctcgtt ttcttgctat
    11341 gcaaatagaa caaatgtatg aaattgtagt acctgaattt gttgaacccg ttttagaaca
    11401 aggtatcaag gataagtctt tggagattga aaattcacat gaaattgcag aagttatcat
    11461 ggtattaagt aatgtatggc tcaacccatt agttgaaatg acggataaag agggtatgga
    11521 aaaacgttgc aatacattca atgccctttt aaatggaata ggcatagaca cattattaga
    11581 cgaagaaaca atttctgcat ttatacagtt ttgtaaataa ctgtatttgt tatccctata
    11641 cgatagactg ctctataaaa gagcagttta tttaaaccat tatataaacc gacggtctgt
    11701 ttgattaaat aaaaaggaga gattttatgc aagcagaaaa aaaacttttt aatcgtgatt
    11761 ttatacttgt tgttatagga cagataattt ctttatttgg aaacgctatc ctacgatttg
    11821 cattaccgta ctatttgtta aaagaaactg gttcatcagc tttgtttggt attgctacag
    11881 cctgctcttt tttacctatg gttattttgt cgctggtagg tggtattatt gctgacagga
    11941 caaataaaag aaacattatg gtcatactag attttgtaac agcaatcata attttagtat
    12001 tttatttgtc attgggaaaa attcctatca tacctttgtt tattgttgct ttgatgttac
    12061 tttatggtat ttctggtgca tatcaaccag cggtacaagc aagtattcct ctattggtat
    12121 catcagataa acttaccgtc ggtaatgcta taataaatca agtaaatact ttagcaaact
    12181 ttatcggacc agctataagc ggagtagttt ttcaatttta tggtatcaat cccattttaa
    12241 ttgttagtat tatatgtttt actttttcag ccattatgga aatttttatc catattccac
    12301 accaaaaaag acctcgtgaa gtaggtgtaa ttgcagttgc taaaaacgat ttaaaagata
    12361 gttatacctt tttgaaaaag gagaaaccca tttttatatc cgtaatcttt ttggtttgca
    12421 ttttcaattt ggttttaagt gctgttttaa ttgttggtct gcctatctta atttctgaag
    12481 ttttaaaaat gccagataca ctatatggtt tttcacaatc agcattatca ttaggtggtt
    12541 tatgtggtgg tattttaaca gctcttgttg cttcaaagtt aaaactaaaa aacctttata
    12601 ccttgttact tatttgttct gtttctgttt tacttatggg aatttcgcta tttttagaat
    12661 tgcctgcaat agtttcctac tgggtagtta cagtgatgtg ctttgtggct atgggtgtat
    12721 ctactatgtt tgttgttcaa atttacactc ttgttcagac acaaacacca ccgcaattag
    12781 taggaaagat aatggctacg ctggtagcag ttgctatgtg cggtcaacct attggacaaa
    12841 tggtttacgg tattttattc gatgtattta tacaagaaac atgggttgtt atgtttgtag
    12901 cttctattgc ttcaatcatg atttcaatat attcaaaaag aatttttcaa aagttagaga
    12961 tataatacaa ggggttaaat agtatgttag gctaaaaaca atatataaaa gaaaatttga
    13021 aatctgctaa aaggatagtg ttataaaaga aagagtaata ttgcagtatt taaggctcgc
    13081 acaaatatat gttttgtgtg ggcttttttc ttaccatttt tctgtatttc taaagggtat
    13141 aaacggtatt ccgtttatat atttcagcat tagcagaaag ggggtgagat tatgaggaca
    13201 tcttctttcg agcatatcgt cagattacag ttcaattctt taatgctgat tgtcattaag
    13261 aacaaagtga aaagtcgaaa cagacagctt gcaagacatt ctaaacgtga agtattgttt
    13321 tgtgaaatag ccgaaacgaa acaagttgaa cgtggaacga ttgatacctg tttttatgat
    13381 tgtgtatcat ttaaagtctt gcactttaca atttatgtat ctgatgaaac acttggtaca
    13441 gttttacata gactgtcaga aaaacagcgt agtgcgattt tattacgtta ttttcaagga
    13501 atgaacgaca gagaaatttc agaactgtac catgtatcac gttctgctat ttgcagtaga
    13561 agaactagag gactaaggaa gttaaaaatg cttttgagcg aaaggaagta aagaaatgag
    13621 taaagattat ggctatccct cttttgaatt gatttgcaaa gcgtcaagcg gagatgaaat
    13681 ggcagttaaa gagattttga agttttatga cggttatatt tctaaattat gcttgcgtcc
    13741 tttttaccac tctgaaagtg gtaaaatcat tatgcaggtt gatgaagaac taaaaggaga
    13801 aattcataca gacatgatga aagcaatatt gaaatttgaa atcggagtga agtaatcaac
    13861 tgtataaaaa aatcgggaga tacacagttt cgtgctatct cccttagtag ttttacggtc
    13921 atatcaaggc tcattcaatc gtggtaaatt cgtggtaaga tgagtgtttt tctatacttc
    13981 aaaatgcttg aatgtatagt gtttatgcgg ataattgaaa attagatata aaatttcaaa
    14041 taaattactg atataaattt acaataagaa ctcttgtttc tacttttttt tatgagaaaa
    14101 tttagttttc aatttattga accattttcg gcttggatct cgacgttttt tgatttgatg
    14161 acccgtcacg tattcttgaa caactgcatt gatgattccg cccaaaatga tgatcgtagc
    14221 agcaaaatta agccaaagca tgagtacaat aaaacttcct atgatctgat aaccgctgat
    14281 cctcgttgca aaatacctag catagatact aaacacttgt gataacagca tccagccgac
    14341 agtagcgaag atcgtgcccg gaatgatcgt cttcaacttc aactgagcat tagggacaat
    14401 taaataaatc aagaacatga tgacaaataa agctaccaat gtgattggcc acttcaaagt
    14461 ttggaactga tcgataaagt tcacgggtat ttgtacgatt ggctgaatag catccaaaat
    14521 cagctgtccc aacccaagaa cgatagttac cccactaata gcaatcatga acaagacaat
    14581 gacaattagt gagaagaaac ggacgatcaa aaaatttttt ctattttcta cgccatacgc
    14641 tttattcatc gcaatttgca aagcattgat actttgactt gcagaccata aagccgctaa
    14701 tgcagaaacg gataacaagc ctcctgaact ttgtgtcaaa aggtctttga ttgcaggacc
    14761 gataaactgg taaacggtct caggaatcac ttctcgtaaa taggtcagta cagtctcttg
    14821 atcgatgtgg agaaacggta aaatattacc aaaagcaata atcaatggaa ataaagacaa
    14881 gagtaaataa taagcaacga cgacagaggt cgtactcatt tctgaatcag tgatgcgctt
    14941 ttgagtcgtc tcaataaagc gcatcagatt ttcattttgt tttattttgt tccaaacttt
    15001 catgtacctc tcccgtacct attgactgga tagatcaata agtgccttct tctccttgtg
    15061 atgtcaaaat tcttggacca tctttagtaa tagccaatgt atgttcatat tggcaagaaa
    15121 gtccgccgtc tcttgtatac gcagtccagc cgttaggatc cattttcatt ttccaagttc
    15181 cggtattaac cataggttcg atcgtgataa ccatgccttc tttcaaccgc aacccttttc
    15241 cagcttctcc gtaatgagga actgctggtt cttcatgaat cgtaggacca acgccatgac
    15301 caataaaatc acgaacgatc gctagatttt cgccttctac ataagtttgg atagcatgac
    15361 cgatgtcacc aatccgattc cctacttgtg cttgctcaat accgatgtac aaagctttct
    15421 tcgttacttc catcaatcgg tcgatctcag gtgtagattc tccaactaca taggcccaac
    15481 aagaatcaga aatgccgcct ttcaaatcga tacacatatc gacttttacc aaatcaccgt
    15541 ttttcaaagg ctttttacga gggaatccat gacaaatttc atcatttata ctgatacaag
    15601 tcgcatattc atacccttca aagccaattt gcgcagcaat tccgccatga ctctcgatat
    15661 agtttctgac aaatacctca atatcccaac ttgtgattcc tggttttata aaatcacgca
    15721 gatttttatg tacatctgct aataaagctc cagattctgc catcatttca atttcacgtg
    15781 ctgattttaa agtaatcata ttttcgtctc ctttttacaa atccctctca ttttaacata
    15841 gcggcataca tttagccaaa tgacattccc caggtgtctc taggttttat tttcttttca
    15901 atgatgtttt ggtataattc ataattgtta aataattata aacgaggagg cacatcatgg
    15961 ctttagcaaa aatcgtatat gctagcatga caggaaacac tgaagaaatc gccgatattg
    16021 ttgcagaagc atttgaaaat ctggatatcg aagtcgaaat caacgaatgt acccaagtag
    16081 atgcagatga attcgaggag gcagatattt gcgtagtagc tacttataca tacgatgatg
    16141 gcgatctgcc tgatgagatc gtagactttt atgaagactt gcaagaattg gatctattgg
    16201 gaaaaattta cggtgtctgc ggatctggtg atacttttta cgatgaattc tgcaaatcgg
    16261 tggatgattt cgatgctgct tttgcaaaaa caggcgcatc aaaaggagcc gaaaatgtta
    16321 aagtggatct gaatgctgaa gaagaagata tcgaaaattt agaagctttc gctaaagaac
    16381 tagtcgcaaa aatttaatat acttgcaata aaagagccat ccagatttat tcaatctgta
    16441 tggctctttt attcattgca ttcatggaag agttcttcgg gagttaaaaa tgcatacgct
    16501 gtaagaaaaa cgaacttttc cttattttga cacatctctt gttacctact gatttgtgga
    16561 acttttgagt atattcaatc gatttcgatt ggttctttga atttcaagat agcatctata
    16621 ctgtcttgga tcatcttttc aatctgttca tcagttggat ggatgatgct tgaagacatc
    16681 aaagctgcga tcccagtagc tatgatccat gtcccattat acaaagattc gatctgctct
    16741 tctgaaagat tcacataagc tggatcgcgt ttcactgtat tcacaaagta agtataagag
    16801 aaattctgca tctcttttcc tccaccatat tcttctaagt acagtgaacg atacaacttc
    16861 ttctctcttc ttgcaaaatg gatataatta aaagcaagat cgataatcgt attccctgta
    16921 tgctcaacag tgaacacttc atattgtaaa tgatcataga tctgctcaaa aagagcttgt
    16981 ctcaagtcat ccatattttt aaactcaaga taaatcggtt gcgtggaaca attcattttg
    17041 ttcgcgatgt ttctcgcagt aaatcgtgta aatccatcgg tggcagctac ttcataagca
    17101 gctgccaaaa tctgttctct agtaatcgtc ttttttcttg ccatattttt taatctcctt
    17161 aattttttta taacgcttcc accattgatt aatttcattg taacatgttt tttt
//
LOCUS       DASJSZ010000275        13213 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.guided.073, whole genome shotgun sequence.
ACCESSION   DASJSZ010000275 DASJSZ010000000
VERSION     DASJSZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.guided.073"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(204..2162)
                     /locus_tag="U4B03_003024"
     CDS             complement(204..2162)
                     /locus_tag="U4B03_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2069778.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="U4B03_003025"
     CDS             complement(2159..3031)
                     /locus_tag="U4B03_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2069779.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="U4B03_003026"
     CDS             complement(3373..4245)
                     /locus_tag="U4B03_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2069780.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="U4B03_003027"
     CDS             4373..4942
                     /locus_tag="U4B03_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN2069781.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="U4B03_003028"
     CDS             5009..5746
                     /locus_tag="U4B03_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2069782.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="U4B03_003029"
     CDS             5977..7245
                     /locus_tag="U4B03_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2069783.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="U4B03_003030"
     CDS             7732..8196
                     /locus_tag="U4B03_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2069784.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="U4B03_003031"
     CDS             8180..8809
                     /locus_tag="U4B03_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2069785.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="U4B03_003032"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="U4B03_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN2069786.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="U4B03_003033"
     CDS             10688..11134
                     /locus_tag="U4B03_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN2069787.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="U4B03_003034"
     CDS             complement(11333..11848)
                     /locus_tag="U4B03_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2069788.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="U4B03_003035"
     CDS             11965..13167
                     /locus_tag="U4B03_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2069789.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DASJSZ010000276        12792 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.guided.077, whole genome shotgun sequence.
ACCESSION   DASJSZ010000276 DASJSZ010000000
VERSION     DASJSZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.guided.077"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(284..520)
                     /locus_tag="U4B03_003036"
     CDS             complement(284..520)
                     /locus_tag="U4B03_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069790.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(703..1089)
                     /locus_tag="U4B03_003037"
     CDS             complement(703..1089)
                     /locus_tag="U4B03_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HEN2069791.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(1071..2399)
                     /locus_tag="U4B03_003038"
     CDS             complement(1071..2399)
                     /locus_tag="U4B03_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN2069792.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(2360..2689)
                     /gene="mobC"
                     /locus_tag="U4B03_003039"
     CDS             complement(2360..2689)
                     /gene="mobC"
                     /locus_tag="U4B03_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEN2069793.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(2947..3317)
                     /locus_tag="U4B03_003040"
                     /pseudo
     CDS             complement(2947..3317)
                     /locus_tag="U4B03_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3462..3647
                     /locus_tag="U4B03_003041"
     CDS             3462..3647
                     /locus_tag="U4B03_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2069794.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3805..3972
                     /locus_tag="U4B03_003042"
     CDS             3805..3972
                     /locus_tag="U4B03_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069795.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            4590..5249
                     /gene="vanR"
                     /locus_tag="U4B03_003043"
     CDS             4590..5249
                     /gene="vanR"
                     /locus_tag="U4B03_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HEN2069796.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRACDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5252..6613
                     /gene="vanS"
                     /locus_tag="U4B03_003044"
     CDS             5252..6613
                     /gene="vanS"
                     /locus_tag="U4B03_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN2069797.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(6704..8005)
                     /locus_tag="U4B03_003045"
     CDS             complement(6704..8005)
                     /locus_tag="U4B03_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2069798.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            8284..9090
                     /locus_tag="U4B03_003046"
     CDS             8284..9090
                     /locus_tag="U4B03_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HEN2069799.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            9108..9815
                     /locus_tag="U4B03_003047"
     CDS             9108..9815
                     /locus_tag="U4B03_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEN2069800.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            9812..10783
                     /locus_tag="U4B03_003048"
     CDS             9812..10783
                     /locus_tag="U4B03_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HEN2069801.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            10776..11804
                     /gene="vanB"
                     /locus_tag="U4B03_003049"
     CDS             10776..11804
                     /gene="vanB"
                     /locus_tag="U4B03_003049"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HEN2069802.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            11810..12418
                     /locus_tag="U4B03_003050"
     CDS             11810..12418
                     /locus_tag="U4B03_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HEN2069803.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(12484..12657)
                     /locus_tag="U4B03_003051"
     CDS             complement(12484..12657)
                     /locus_tag="U4B03_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069804.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         3385 a         2824 c         3201 g         3382 t
ORIGIN      
        1 ggcgcggcgg ccatgtggtc gcttgcgtcc ggcgtctttt ctttttgcct attcctacgc
       61 atttagaacc cggtattgtc ggggatagga tgaagtattc gcttgcccct gcaaacatgc
      121 gggagaagcc ttgcagcggg cggttttcaa tcccttaaat gcgtaggttg cagctataat
      181 tagacccctg aaacagatat agccgcgcag cgggtgtatc tgtttcaggg gtgtgcaggt
      241 gatagccgca aagcggcgta agagggtgca gccctcttgc cgtctatctc tgttccagcg
      301 ggaagctcgg caatgcttcc agcagtttca gggaaatgcg ctgccgtata tcttcgtcaa
      361 tgactttctt ctttttgccg tttgcctgtc tgatctccac cgtggaaagc tcgttgatgt
      421 agtcagcata atgggccaac actttttccg tggcccacct ctcaccggca acggcggctt
      481 tgatcgtttc atagtccagc agcttttctt gtccgtccat cctgataccc ccgtcagact
      541 gattttttct atgataccat attcggaagc atggaaccag agaaaacggc gcttttctgc
      601 tggtaaattc tacgcattta gaacagcact tcggcgggga taggataaaa cacccacata
      661 ccccgctgac catgccggaa aagccttgaa atataagggc ttttacagct ctaagtgcgt
      721 aggtttccag tatcttttcc gctggcgctc ggcctctttg cggcggcgca tccgtttgga
      781 gcaggccgcg cagtatttgg cccggttaga cttcggcgtg aacgtggccc cgcactcaca
      841 gcagcgtttc acttcgtccc ggtctttggt gatctcggca tacagggccg catccagcgg
      901 gaggacagcg gccttaaacc agcggcacag aagcgaatag gaaatgctct gtacgcaaat
      961 acattcttcg ccgtcgtcca gcaacaggca gttgccgtca caatagttgc agcactcatg
     1021 cgtcagcttc cgggccttgc gatattgagg gtaattcatt cgtggaatac tcatagctct
     1081 tgcgtccatt cctttcccgg cgtcatacgc aaaatgccgt ccacattctg cttgatgatg
     1141 ccgtattcct gcatctgctt tttcgcttcg ccgtacttct catacagccg ttccttttcc
     1201 tcggccaatc gcttgtactc ggctttcagg gcgtacagat cggggagctt tttcacaccc
     1261 tgtcccttta gggcctttgc cgcagcttca tacaaaatca acgtgctttc atgctcacgc
     1321 cggaattttg ccttatccgc tgcattgcgg tattccatag ccacgggcca gagctgcttg
     1381 taggtagaaa tattctttat cagcaccgcc atatccgcaa gccggtgttc catagctttg
     1441 agggtagcgg cggcctcgtc gctggcggcg gtgatctcgg ccactttgct ttccaaatcc
     1501 gggtaggtgt ctatcccatg ttcagtcagg aaattcaaag tctttgcggc ctgcttcaaa
     1561 ttgtggagct tcgcccacct ctcatagcct gccgactgct gggccttaat gttgttttcc
     1621 agatcaatcc gcaaagaaat ccccttgcgc tctttgggcg ctctggtgat aacgcggcct
     1681 ttgatacgct ctttgatggc ttcctcggtg taggcttccc ccaatgtctt gcagcgggta
     1741 aagcgttcct gtccgggagc gcggaaagaa ataaactttc ccggcttgat ctcatagccc
     1801 tgcgcctgca tgagccgcag gaaatcatca aaatcttttg cctgcgggat agcggcgtca
     1861 atggctgctt tgagcttcgc tttccagctc gtccctttcc gctgggcgtc ccactcggca
     1921 tagctcttgc ccttactctg gccgggcttc accacggaca atccgttttc cttgcacagc
     1981 ctgtcgctgg tgcgccggat ataggcatag ctctgtttgt tggaaatgta cttgcgctga
     2041 tttaccatat ccacggcaca gaaaataatg tgattgtgga cgtggccctt atcaatatgg
     2101 gtagtcagca catagggata tttcccttgc agcacttcct cggcaagctg cttcccgatc
     2161 tcatgggcct gttccggcgt ggcttcgccg ggggcaaagg attgtatcag gtggtggccc
     2221 agattattgc ccttttggta tgcctgcgcc agcagcattt caaattcaat gtccgctgtt
     2281 tcataagagc aggcaaagga ggccaccagc agcttgccgt cagttttgtc cgggttctcg
     2341 atgtagtcaa gggctttttt cagcgttgac ttgataggat gtatctttgt aactgccata
     2401 tctgcgccag cgcccccttt atatcctcga tgtcctgtgc gtacaccggc cccataccgt
     2461 tcagcctctt tgcaatctga ttgacgttga cgccgatctt ctgtatctcg gcggtctgcg
     2521 cccggatgtc gctggtgtcc atgttgacga tatagccgtc aatgagcatc ttccgggcgt
     2581 aggctgaaaa gttcttcgta tgcagcagcg ccattttctg ttcaatcata gcccgttcct
     2641 ccggcgtcac agggacgcgc agcacgattt ttcgctttct gtttgccatg cttcaccgtc
     2701 ctttccattc aggggtttgg ggatttcccc aacaagcaaa acggacgcgg cccggcaggg
     2761 acggggcagg ccgtttttcc gggagtggct acactcccta tgcttgctac gttactgttt
     2821 ctccactaac aagacgtttc caaagcccca aactacccgc aaaatagccc tctggaaaga
     2881 aaaaagtgca aaaaaataga agctacgctg tgcagctccc gctaaaagta aaacagagga
     2941 tgccagttac acatcctccg tttcattctt cttcggcttt tcaattagta tggcgctgtc
     3001 aatcgcgccc tcgatgatcg gcaggtattc ttcggggcag agtttcagct tgtggctgac
     3061 cctttgccgc agttcgcttt cctcccgcat gatctccggg ttaaagtagc gttccacagg
     3121 aagcccgcag gctttaataa gctgtatgac aaccggcaga ctgggaatgg cccgtcgttt
     3181 tcgatgttgg caagatagcg ccagtcaata tttattttct ctgccattgc tttgcgcgaa
     3241 acgcgctttg cttgccgggc cgctttcaca tccgcgccga aagtttcaaa gccgggacaa
     3301 tcttcaatat tcgccatatc gcacctccca cttacattgt atatttcata ctctgcccgt
     3361 ggaatgttgg tatatgcacc tttcttaaag tttatatttc acattttgtt tcttttattc
     3421 ttcgagcaaa agaattataa tgtaccctgt ggaggtgtac tatggactac atgacgttga
     3481 aagaggcagg cgagaaatgg ggtgtgcccc cacgatggat aaactactat tgtgctgcgg
     3541 gacgcatccc cggcgctgta aagatggcga cgatctggct cattcccaaa actgcggaaa
     3601 agccgattga caggcgtaca aagaaaggaa aggcggttac tgaatagtat aatgaaaccg
     3661 gcgcattttg gatatggttt tgaagagaga gaaagcatgg tattttgtca cggaaaaact
     3721 ataagatgat agatggcagg ctgctccaga ccgataaaaa gtattctcat ctgaaacaga
     3781 aagaaaagat ttacacatgg atgtatgaag aagcgaaact ttatcatgac agaaatggaa
     3841 aatgcccgaa acaaagacga ggaagctgct gctgtatcag ccgtatatgg tagaattgaa
     3901 aaggccggca tctggattct ttatggtgaa gtctccagac attggataac tgctggtcta
     3961 atgcgccttt gagtatgggg tgattgattt taataccggg gaggtacggc tttttgagga
     4021 aaatattctg ggatttacat ttggcgatta ttacatttcc tgtaactgca aggacacgga
     4081 tgatgaagat atgaggaata tagacttgct gattttctac tgtcttgaac ggtattagga
     4141 ctgaatctgc tgcgcggagt gcggcaagag cgaccggagg gagtacacac aggccctgcc
     4201 tgtgtgaaaa gccataccgc aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag
     4261 gcttggggtg tccccaacaa gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag
     4321 aatatccgta aaggatattc tgatccattg cttgccagta ttatcgggat gttttgacaa
     4381 ccgctgtaat cgaaacatcc caaacattaa gaaggtgtta attattatta tcttgattta
     4441 attcgtttaa accaataata tagatacata caccatgaaa agaagcgagt atcccaatga
     4501 cggatgatta aaatccgaaa agaaatcgct ttgaaactac agggaaacta cagactgtta
     4561 tgttatcttc ttaaaatgga gggattttta tgtcgatacg aattctactt gtcgaggatg
     4621 atgatcatat ctgtaataca gtaagggcgt ttctggctga ggcaggatat caggtggatg
     4681 cctgcacaga tggaaatgag gcatacacca agttttacga aaacacttat caactggtta
     4741 ttcttgatat tatgctgccc ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga
     4801 aaaatgatac tcccattctg atgatgacag ccctgtcgga tgacgaaaac caaatccggg
     4861 cgtgtgatgc agaggcagac gactatgtaa caaagccatt caagatgcag attttactaa
     4921 agcgggtgga agccctgctg cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca
     4981 ggctgacact tctgccggag gatttcacgg tactttgtga cggtacagag ctgcccctga
     5041 cacgaaaaga atttgaaatc cttttgctgc tggtgcagaa caaaggcaga accttaacgc
     5101 atgaaatcat tttgtcacgt atatggggat atgactttga gggtgatggc agcacagtcc
     5161 acactcatat caaaaatttg cgggccaagc tgccggaaaa tatcatcaaa accattcgcg
     5221 gtgtaggata ccgattggag gaatcataat aatggaaaga aaagggattt tcattaaggt
     5281 tttttcctat acgatcattg tcctgctact gcttgtcggt gtaacagcaa cactgtttgc
     5341 acagcaattt gtgtcttatt tcagagtgat ggaattacag caaacagtaa aatcctatca
     5401 gccattggtg gaactgattc agaatagcga taggcttgat attcaagagg tggcagggct
     5461 gtttcactac aataaccaat cctttgagtt ttatattgaa gataaagagg gaagcgtact
     5521 ctatgccaca ccaaatgcca atacatcaaa tagttttaga cccaactttc tttatgtggt
     5581 acatagagat gataatattt cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta
     5641 tcaagggctg acaattcggg gaattgttat gattgcgata atggttgtat tcagcctttt
     5701 atgcgcgtat atctttgcgc ggcaaatgac aacgccgatc aaagccttag cggacagtgc
     5761 gaataaaatg gcaaacctga aagatgtacc gccgccgctg gagcgaaagg atgagcttgg
     5821 cgcactggct catgatatgc attccatgta tgtcaggctg aaagaaacca tcgcaaggct
     5881 ggaggatgaa atcgcaaggg aacatgagtt ggaggaaaca cagcgatatt tctttgcggc
     5941 agcttctcat gagctaaaaa cgcccatcgc ggctacaagc gttctgttgg agggaatgct
     6001 tgaaaatatc ggtgactaca aagatcattc taagtatctg cgcgaatgca tcaaaatgat
     6061 ggataggcag ggcaaaatca tttccgaaat actggagctt gtcagcctga atgatgggag
     6121 aatcgtaccc atagctgaac cgttggacat agggcgcacg gttgccgagt tgctgcccga
     6181 ttttcaaacc ttggcagagg caaacaacca gcggttcgtc acagatattc cagccgggca
     6241 aattgtcctg tccgatccga ggctgctcca aaaggcacta tccaatgtca tattgaatgc
     6301 agttcagaac acgccgcagg gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa
     6361 atgccgcctt tttgttttga acatgggcgt tcacattgat gatactgcgc ttccaaggct
     6421 gttcacccca ttctatcgca ttgatcaggc gcgaagcaga aaaagtgggc gaagcagaaa
     6481 aagtgggcga agcggtttag gacttgccat cgtacaaaaa acgctggatg ccatgagcct
     6541 ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc tggctggatt taccgctcac
     6601 atcaacattg taaatattta aaacttaaat gattttgggc tctttgtcaa ataggactga
     6661 tagagtgaga taagaacatc taaggtagaa ttccttttct actttagatg ttttttgatt
     6721 cataggtaat cagattattt agtaagaaaa ttctggtctt cattttccga aagcttcgaa
     6781 agccataagc gactcgtttc aatgctttga tgtgggtatg cagattttct attttggcat
     6841 ttgaataagg caattcgagg gctaaacgaa tgccggattc atagtttata aaagcttttt
     6901 ttatagaatg cagttctttt ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag
     6961 tgtccgcctc tttgtcgtga aacgcttgaa gaatttcttg atagtaaaaa taggcgattc
     7021 gtaattcgtc agagatcgaa agcagacgat ccaccacttc actttcggtg agatacgccc
     7081 aaccaaaatt gcgccatttg gtccatttgt cataaacaag cttgtcctgt cgtttgatca
     7141 gaagccgaga caacgccttt aattgtcgat aatatttggc ttgtgttgga tcgtttcgat
     7201 caaagctttt catgattcgt acgcgaatat cctcaaattt acgaatgaga tgtttgacaa
     7261 tgtgaaaacg atccacaacg agttttgcct ttggaaaaca ttcctttatc agagaggcat
     7321 aagaaacatt catgtccgtc acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt
     7381 gcataaaata agaaacgaga gcctttttct tccgtgaagg aagaatatcc agtaattttc
     7441 cagtatcgcc atccatacaa gaaaaggagt agggaccgac ttgatttttc agtgaacgaa
     7501 attcatccat taataaacaa cgcggtaacc aatttctttt tacttttgtc atggggatca
     7561 gagaagcgag tgtccgagaa acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg
     7621 tcattgcttg tttttcagtt agctcttcta aaattttata gcgcacttgt ttagcgataa
     7681 agcagtttct atcgacaatg taggtattcg ccgtaaaata ggtgtggcag ccacgacagt
     7741 ggtagcgttg tttcgctaaa gcaagataag tcagttggtt cgcacattta ttaagttgaa
     7801 tgagcgattt tttctttccg ttcttaacga tcatgccttc atgattcatc tgtttacaca
     7861 aaggacagcg acgaatttct ttacttaacg tcgcagaaac aacaagactc ttgatattct
     7921 taatgctctt cgattccaca tttgtgatga aaatgttttt atctttgatt ccaagtagta
     7981 gtttgatatg atgaagatca ggcatacaga acgctccttt gattgtgttg tggtgattca
     8041 attctaacgg agcttctgta tgttttgttt tttcatgctc aaaagggacc gatagaatga
     8101 ttttctatca gtcctagaaa ttatagagcc gattttgacc gacaggtata accctgccgg
     8161 tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac agatgcgctt
     8221 gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga ggcattcgat
     8281 caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca tatgaaacaa
     8341 tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt ctgcacgctg
     8401 tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc catccctgca
     8461 actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc tcttgccacc
     8521 gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag gcagaacccc
     8581 atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg gatagatatt
     8641 cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg agtttacccg
     8701 attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga tgaaaaaatt
     8761 gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc agaaacttgg
     8821 gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat caatgcggac
     8881 ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag ctatcgtttt
     8941 ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag caacgagccg
     9001 tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca aggggtttgc
     9061 cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg gacagaaaaa
     9121 gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga aaaatgtgct
     9181 tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg gaaaaatcgc
     9241 tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc ggatttgaca
     9301 tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc ttaaacggct
     9361 tattgataag accgggggaa accttttctt tctggttgtt cctgcatacg ccattgacaa
     9421 ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt gacgagatta
     9481 aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga aacgataccg
     9541 actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt caggtgttcg
     9601 ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag tatgtccgtg
     9661 aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc ttaggtaccg
     9721 gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt tatcccctcc
     9781 cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca ttactgtttt
     9841 tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt ttcatattat
     9901 ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg ctggcaatca
     9961 atgcgttagc gtaagccata agtccgaggt ttccgaggcg acaattcttg cgctgagaaa
    10021 ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg atacgactgc
    10081 cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca gcgttgcgga
    10141 ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca tgcacgccgt
    10201 ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg atatgactgt
    10261 gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc ggggatttgg
    10321 atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg tccagcttga
    10381 tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg cggatacccg
    10441 ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt taatcaacac
    10501 cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa gcggaaagct
    10561 gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata ccgattgctc
    10621 gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga atgtgatcat
    10681 cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag aaaaaacaat
    10741 caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa gtcgcaatca
    10801 tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa agccgcaata gaaattgctg
    10861 cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa aacggtgtat
    10921 ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc gccatactct
    10981 ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac gaaacacggc
    11041 gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt gcgatacagg
    11101 ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc tccgcagctt
    11161 gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc gttcccgaat
    11221 ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac cctgtctttg
    11281 tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt acggaagaac
    11341 ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt gagcaagcga
    11401 tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg attgtcggcg
    11461 aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa aacgagccgg
    11521 aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc gaggaacgaa
    11581 atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga gggcttgccc
    11641 gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc aataccctgc
    11701 ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga atcacgcttc
    11761 ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta tggaaaatgg
    11821 ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt acgccacatg
    11881 ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca tcggcacaaa
    11941 ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg gctatggctt
    12001 gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc gttgggcagc
    12061 gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc gagccgagtt
    12121 gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg caattgatct
    12181 tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact tcgattttat
    12241 ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac aaaatcgaag
    12301 atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg aatggtggca
    12361 ctataagttg attgatgagc cataccccga tacctatttt aattttgccg tttcataatg
    12421 aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt catggacaat
    12481 cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat agtattcaat
    12541 ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca cgcccatttt
    12601 gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg gcagcaaaag
    12661 gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg ctctaatcca
    12721 cttccaccac tcaaaaaaag ccttgcccct ccatgaactg aaccccaaaa gttgaactat
    12781 ttaatggact gt
//
LOCUS       DASJSZ010000277        12792 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.guided.078, whole genome shotgun sequence.
ACCESSION   DASJSZ010000277 DASJSZ010000000
VERSION     DASJSZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.guided.078"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(284..520)
                     /locus_tag="U4B03_003052"
     CDS             complement(284..520)
                     /locus_tag="U4B03_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2069805.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(703..1089)
                     /locus_tag="U4B03_003053"
     CDS             complement(703..1089)
                     /locus_tag="U4B03_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HEN2069806.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(1071..2399)
                     /locus_tag="U4B03_003054"
     CDS             complement(1071..2399)
                     /locus_tag="U4B03_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN2069807.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(2360..2689)
                     /gene="mobC"
                     /locus_tag="U4B03_003055"
     CDS             complement(2360..2689)
                     /gene="mobC"
                     /locus_tag="U4B03_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEN2069808.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(2947..3317)
                     /locus_tag="U4B03_003056"
                     /pseudo
     CDS             complement(2947..3317)
                     /locus_tag="U4B03_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3462..3647
                     /locus_tag="U4B03_003057"
     CDS             3462..3647
                     /locus_tag="U4B03_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2069809.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3805..3972
                     /locus_tag="U4B03_003058"
     CDS             3805..3972
                     /locus_tag="U4B03_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069810.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            4590..5249
                     /gene="vanR"
                     /locus_tag="U4B03_003059"
     CDS             4590..5249
                     /gene="vanR"
                     /locus_tag="U4B03_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HEN2069811.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5252..6613
                     /gene="vanS"
                     /locus_tag="U4B03_003060"
     CDS             5252..6613
                     /gene="vanS"
                     /locus_tag="U4B03_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN2069812.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(6704..8005)
                     /locus_tag="U4B03_003061"
     CDS             complement(6704..8005)
                     /locus_tag="U4B03_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2069813.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            8284..9090
                     /locus_tag="U4B03_003062"
     CDS             8284..9090
                     /locus_tag="U4B03_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HEN2069814.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            9108..9815
                     /locus_tag="U4B03_003063"
     CDS             9108..9815
                     /locus_tag="U4B03_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEN2069815.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            9812..10783
                     /locus_tag="U4B03_003064"
     CDS             9812..10783
                     /locus_tag="U4B03_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HEN2069816.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            10776..11804
                     /gene="vanB"
                     /locus_tag="U4B03_003065"
     CDS             10776..11804
                     /gene="vanB"
                     /locus_tag="U4B03_003065"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HEN2069817.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            11810..12418
                     /locus_tag="U4B03_003066"
     CDS             11810..12418
                     /locus_tag="U4B03_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HEN2069818.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(12484..12657)
                     /locus_tag="U4B03_003067"
     CDS             complement(12484..12657)
                     /locus_tag="U4B03_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069819.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         3385 a         2824 c         3200 g         3383 t
ORIGIN      
        1 ggcgcggcgg ccatgtggtc gcttgcgtcc ggcgtctttt ctttttgcct attcctacgc
       61 atttagaacc cggtattgtc ggggatagga tgaagtattc gcttgcccct gcaaacatgc
      121 gggagaagcc ttgcagcggg cggttttcaa tcccttaaat gcgtaggttg cagctataat
      181 tagacccctg aaacagatat agccgcgcag cgggtgtatc tgtttcaggg gtgtgcaggt
      241 gatagccgca aagcggcgta agagggtgca gccctcttgc cgtctatctc tgttccagcg
      301 ggaagctcgg caatgcttcc agcagtttca gggaaatgcg ctgccgtata tcttcgtcaa
      361 tgactttctt ctttttgccg tttgcctgtc tgatctccac cgtggaaagc tcgttgatgt
      421 agtcagcata atgggccaac actttttccg tggcccacct ctcaccggca acggcggctt
      481 tgatcgtttc atagtccagc agcttttctt gtccgtccat cctgataccc ccgtcagact
      541 gattttttct atgataccat attcggaagc atggaaccag agaaaacggc gcttttctgc
      601 tggtaaattc tacgcattta gaacagcact tcggcgggga taggataaaa cacccacata
      661 ccccgctgac catgccggaa aagccttgaa atataagggc ttttacagct ctaagtgcgt
      721 aggtttccag tatcttttcc gctggcgctc ggcctctttg cggcggcgca tccgtttgga
      781 gcaggccgcg cagtatttgg cccggttaga cttcggcgtg aacgtggccc cgcactcaca
      841 gcagcgtttc acttcgtccc ggtctttggt gatctcggca tacagggccg catccagcgg
      901 gaggacagcg gccttaaacc agcggcacag aagcgaatag gaaatgctct gtacgcaaat
      961 acattcttcg ccgtcgtcca gcaacaggca gttgccgtca caatagttgc agcactcatg
     1021 cgtcagcttc cgggccttgc gatattgagg gtaattcatt cgtggaatac tcatagctct
     1081 tgcgtccatt cctttcccgg cgtcatacgc aaaatgccgt ccacattctg cttgatgatg
     1141 ccgtattcct gcatctgctt tttcgcttcg ccgtacttct catacagccg ttccttttcc
     1201 tcggccaatc gcttgtactc ggctttcagg gcgtacagat cggggagctt tttcacaccc
     1261 tgtcccttta gggcctttgc cgcagcttca tacaaaatca acgtgctttc atgctcacgc
     1321 cggaattttg ccttatccgc tgcattgcgg tattccatag ccacgggcca gagctgcttg
     1381 taggtagaaa tattctttat cagcaccgcc atatccgcaa gccggtgttc catagctttg
     1441 agggtagcgg cggcctcgtc gctggcggcg gtgatctcgg ccactttgct ttccaaatcc
     1501 gggtaggtgt ctatcccatg ttcagtcagg aaattcaaag tctttgcggc ctgcttcaaa
     1561 ttgtggagct tcgcccacct ctcatagcct gccgactgct gggccttaat gttgttttcc
     1621 agatcaatcc gcaaagaaat ccccttgcgc tctttgggcg ctctggtgat aacgcggcct
     1681 ttgatacgct ctttgatggc ttcctcggtg taggcttccc ccaatgtctt gcagcgggta
     1741 aagcgttcct gtccgggagc gcggaaagaa ataaactttc ccggcttgat ctcatagccc
     1801 tgcgcctgca tgagccgcag gaaatcatca aaatcttttg cctgcgggat agcggcgtca
     1861 atggctgctt tgagcttcgc tttccagctc gtccctttcc gctgggcgtc ccactcggca
     1921 tagctcttgc ccttactctg gccgggcttc accacggaca atccgttttc cttgcacagc
     1981 ctgtcgctgg tgcgccggat ataggcatag ctctgtttgt tggaaatgta cttgcgctga
     2041 tttaccatat ccacggcaca gaaaataatg tgattgtgga cgtggccctt atcaatatgg
     2101 gtagtcagca catagggata tttcccttgc agcacttcct cggcaagctg cttcccgatc
     2161 tcatgggcct gttccggcgt ggcttcgccg ggggcaaagg attgtatcag gtggtggccc
     2221 agattattgc ccttttggta tgcctgcgcc agcagcattt caaattcaat gtccgctgtt
     2281 tcataagagc aggcaaagga ggccaccagc agcttgccgt cagttttgtc cgggttctcg
     2341 atgtagtcaa gggctttttt cagcgttgac ttgataggat gtatctttgt aactgccata
     2401 tctgcgccag cgcccccttt atatcctcga tgtcctgtgc gtacaccggc cccataccgt
     2461 tcagcctctt tgcaatctga ttgacgttga cgccgatctt ctgtatctcg gcggtctgcg
     2521 cccggatgtc gctggtgtcc atgttgacga tatagccgtc aatgagcatc ttccgggcgt
     2581 aggctgaaaa gttcttcgta tgcagcagcg ccattttctg ttcaatcata gcccgttcct
     2641 ccggcgtcac agggacgcgc agcacgattt ttcgctttct gtttgccatg cttcaccgtc
     2701 ctttccattc aggggtttgg ggatttcccc aacaagcaaa acggacgcgg cccggcaggg
     2761 acggggcagg ccgtttttcc gggagtggct acactcccta tgcttgctac gttactgttt
     2821 ctccactaac aagacgtttc caaagcccca aactacccgc aaaatagccc tctggaaaga
     2881 aaaaagtgca aaaaaataga agctacgctg tgcagctccc gctaaaagta aaacagagga
     2941 tgccagttac acatcctccg tttcattctt cttcggcttt tcaattagta tggcgctgtc
     3001 aatcgcgccc tcgatgatcg gcaggtattc ttcggggcag agtttcagct tgtggctgac
     3061 cctttgccgc agttcgcttt cctcccgcat gatctccggg ttaaagtagc gttccacagg
     3121 aagcccgcag gctttaataa gctgtatgac aaccggcaga ctgggaatgg cccgtcgttt
     3181 tcgatgttgg caagatagcg ccagtcaata tttattttct ctgccattgc tttgcgcgaa
     3241 acgcgctttg cttgccgggc cgctttcaca tccgcgccga aagtttcaaa gccgggacaa
     3301 tcttcaatat tcgccatatc gcacctccca cttacattgt atatttcata ctctgcccgt
     3361 ggaatgttgg tatatgcacc tttcttaaag tttatatttc acattttgtt tcttttattc
     3421 ttcgagcaaa agaattataa tgtaccctgt ggaggtgtac tatggactac atgacgttga
     3481 aagaggcagg cgagaaatgg ggtgtgcccc cacgatggat aaactactat tgtgctgcgg
     3541 gacgcatccc cggcgctgta aagatggcga cgatctggct cattcccaaa actgcggaaa
     3601 agccgattga caggcgtaca aagaaaggaa aggcggttac tgaatagtat aatgaaaccg
     3661 gcgcattttg gatatggttt tgaagagaga gaaagcatgg tattttgtca cggaaaaact
     3721 ataagatgat agatggcagg ctgctccaga ccgataaaaa gtattctcat ctgaaacaga
     3781 aagaaaagat ttacacatgg atgtatgaag aagcgaaact ttatcatgac agaaatggaa
     3841 aatgcccgaa acaaagacga ggaagctgct gctgtatcag ccgtatatgg tagaattgaa
     3901 aaggccggca tctggattct ttatggtgaa gtctccagac attggataac tgctggtcta
     3961 atgcgccttt gagtatgggg tgattgattt taataccggg gaggtacggc tttttgagga
     4021 aaatattctg ggatttacat ttggcgatta ttacatttcc tgtaactgca aggacacgga
     4081 tgatgaagat atgaggaata tagacttgct gattttctac tgtcttgaac ggtattagga
     4141 ctgaatctgc tgcgcggagt gcggcaagag cgaccggagg gagtacacac aggccctgcc
     4201 tgtgtgaaaa gccataccgc aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag
     4261 gcttggggtg tccccaacaa gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag
     4321 aatatccgta aaggatattc tgatccattg cttgccagta ttatcgggat gttttgacaa
     4381 ccgctgtaat cgaaacatcc caaacattaa gaaggtgtta attattatta tcttgattta
     4441 attcgtttaa accaataata tagatacata caccatgaaa agaagcgagt atcccaatga
     4501 cggatgatta aaatccgaaa agaaatcgct ttgaaactac agggaaacta cagactgtta
     4561 tgttatcttc ttaaaatgga gggattttta tgtcgatacg aattctactt gtcgaggatg
     4621 atgatcatat ctgtaataca gtaagggcgt ttctggctga ggcaggatat caggtggatg
     4681 cctgcacaga tggaaatgag gcatacacca agttttacga aaacacttat caactggtta
     4741 ttcttgatat tatgctgccc ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga
     4801 aaaatgatac tcccattctg atgatgacag ccctgtcgga tgacgaaaac caaatccggg
     4861 cgtttgatgc agaggcagac gactatgtaa caaagccatt caagatgcag attttactaa
     4921 agcgggtgga agccctgctg cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca
     4981 ggctgacact tctgccggag gatttcacgg tactttgtga cggtacagag ctgcccctga
     5041 cacgaaaaga atttgaaatc cttttgctgc tggtgcagaa caaaggcaga accttaacgc
     5101 atgaaatcat tttgtcacgt atatggggat atgactttga gggtgatggc agcacagtcc
     5161 acactcatat caaaaatttg cgggccaagc tgccggaaaa tatcatcaaa accattcgcg
     5221 gtgtaggata ccgattggag gaatcataat aatggaaaga aaagggattt tcattaaggt
     5281 tttttcctat acgatcattg tcctgctact gcttgtcggt gtaacagcaa cactgtttgc
     5341 acagcaattt gtgtcttatt tcagagtgat ggaattacag caaacagtaa aatcctatca
     5401 gccattggtg gaactgattc agaatagcga taggcttgat attcaagagg tggcagggct
     5461 gtttcactac aataaccaat cctttgagtt ttatattgaa gataaagagg gaagcgtact
     5521 ctatgccaca ccaaatgcca atacatcaaa tagttttaga cccaactttc tttatgtggt
     5581 acatagagat gataatattt cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta
     5641 tcaagggctg acaattcggg gaattgttat gattgcgata atggttgtat tcagcctttt
     5701 atgcgcgtat atctttgcgc ggcaaatgac aacgccgatc aaagccttag cggacagtgc
     5761 gaataaaatg gcaaacctga aagatgtacc gccgccgctg gagcgaaagg atgagcttgg
     5821 cgcactggct catgatatgc attccatgta tgtcaggctg aaagaaacca tcgcaaggct
     5881 ggaggatgaa atcgcaaggg aacatgagtt ggaggaaaca cagcgatatt tctttgcggc
     5941 agcttctcat gagctaaaaa cgcccatcgc ggctacaagc gttctgttgg agggaatgct
     6001 tgaaaatatc ggtgactaca aagatcattc taagtatctg cgcgaatgca tcaaaatgat
     6061 ggataggcag ggcaaaatca tttccgaaat actggagctt gtcagcctga atgatgggag
     6121 aatcgtaccc atagctgaac cgttggacat agggcgcacg gttgccgagt tgctgcccga
     6181 ttttcaaacc ttggcagagg caaacaacca gcggttcgtc acagatattc cagccgggca
     6241 aattgtcctg tccgatccga ggctgctcca aaaggcacta tccaatgtca tattgaatgc
     6301 agttcagaac acgccgcagg gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa
     6361 atgccgcctt tttgttttga acatgggcgt tcacattgat gatactgcgc ttccaaggct
     6421 gttcacccca ttctatcgca ttgatcaggc gcgaagcaga aaaagtgggc gaagcagaaa
     6481 aagtgggcga agcggtttag gacttgccat cgtacaaaaa acgctggatg ccatgagcct
     6541 ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc tggctggatt taccgctcac
     6601 atcaacattg taaatattta aaacttaaat gattttgggc tctttgtcaa ataggactga
     6661 tagagtgaga taagaacatc taaggtagaa ttccttttct actttagatg ttttttgatt
     6721 cataggtaat cagattattt agtaagaaaa ttctggtctt cattttccga aagcttcgaa
     6781 agccataagc gactcgtttc aatgctttga tgtgggtatg cagattttct attttggcat
     6841 ttgaataagg caattcgagg gctaaacgaa tgccggattc atagtttata aaagcttttt
     6901 ttatagaatg cagttctttt ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag
     6961 tgtccgcctc tttgtcgtga aacgcttgaa gaatttcttg atagtaaaaa taggcgattc
     7021 gtaattcgtc agagatcgaa agcagacgat ccaccacttc actttcggtg agatacgccc
     7081 aaccaaaatt gcgccatttg gtccatttgt cataaacaag cttgtcctgt cgtttgatca
     7141 gaagccgaga caacgccttt aattgtcgat aatatttggc ttgtgttgga tcgtttcgat
     7201 caaagctttt catgattcgt acgcgaatat cctcaaattt acgaatgaga tgtttgacaa
     7261 tgtgaaaacg atccacaacg agttttgcct ttggaaaaca ttcctttatc agagaggcat
     7321 aagaaacatt catgtccgtc acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt
     7381 gcataaaata agaaacgaga gcctttttct tccgtgaagg aagaatatcc agtaattttc
     7441 cagtatcgcc atccatacaa gaaaaggagt agggaccgac ttgatttttc agtgaacgaa
     7501 attcatccat taataaacaa cgcggtaacc aatttctttt tacttttgtc atggggatca
     7561 gagaagcgag tgtccgagaa acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg
     7621 tcattgcttg tttttcagtt agctcttcta aaattttata gcgcacttgt ttagcgataa
     7681 agcagtttct atcgacaatg taggtattcg ccgtaaaata ggtgtggcag ccacgacagt
     7741 ggtagcgttg tttcgctaaa gcaagataag tcagttggtt cgcacattta ttaagttgaa
     7801 tgagcgattt tttctttccg ttcttaacga tcatgccttc atgattcatc tgtttacaca
     7861 aaggacagcg acgaatttct ttacttaacg tcgcagaaac aacaagactc ttgatattct
     7921 taatgctctt cgattccaca tttgtgatga aaatgttttt atctttgatt ccaagtagta
     7981 gtttgatatg atgaagatca ggcatacaga acgctccttt gattgtgttg tggtgattca
     8041 attctaacgg agcttctgta tgttttgttt tttcatgctc aaaagggacc gatagaatga
     8101 ttttctatca gtcctagaaa ttatagagcc gattttgacc gacaggtata accctgccgg
     8161 tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac agatgcgctt
     8221 gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga ggcattcgat
     8281 caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca tatgaaacaa
     8341 tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt ctgcacgctg
     8401 tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc catccctgca
     8461 actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc tcttgccacc
     8521 gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag gcagaacccc
     8581 atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg gatagatatt
     8641 cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg agtttacccg
     8701 attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga tgaaaaaatt
     8761 gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc agaaacttgg
     8821 gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat caatgcggac
     8881 ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag ctatcgtttt
     8941 ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag caacgagccg
     9001 tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca aggggtttgc
     9061 cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg gacagaaaaa
     9121 gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga aaaatgtgct
     9181 tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg gaaaaatcgc
     9241 tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc ggatttgaca
     9301 tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc ttaaacggct
     9361 tattgataag accgggggaa accttttctt tctggttgtt cctgcatacg ccattgacaa
     9421 ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt gacgagatta
     9481 aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga aacgataccg
     9541 actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt caggtgttcg
     9601 ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag tatgtccgtg
     9661 aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc ttaggtaccg
     9721 gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt tatcccctcc
     9781 cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca ttactgtttt
     9841 tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt ttcatattat
     9901 ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg ctggcaatca
     9961 atgcgttagc gtaagccata agtccgaggt ttccgaggcg acaattcttg cgctgagaaa
    10021 ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg atacgactgc
    10081 cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca gcgttgcgga
    10141 ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca tgcacgccgt
    10201 ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg atatgactgt
    10261 gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc ggggatttgg
    10321 atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg tccagcttga
    10381 tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg cggatacccg
    10441 ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt taatcaacac
    10501 cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa gcggaaagct
    10561 gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata ccgattgctc
    10621 gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga atgtgatcat
    10681 cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag aaaaaacaat
    10741 caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa gtcgcaatca
    10801 tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa agccgcaata gaaattgctg
    10861 cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa aacggtgtat
    10921 ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc gccatactct
    10981 ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac gaaacacggc
    11041 gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt gcgatacagg
    11101 ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc tccgcagctt
    11161 gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc gttcccgaat
    11221 ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac cctgtctttg
    11281 tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt acggaagaac
    11341 ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt gagcaagcga
    11401 tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg attgtcggcg
    11461 aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa aacgagccgg
    11521 aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc gaggaacgaa
    11581 atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga gggcttgccc
    11641 gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc aataccctgc
    11701 ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga atcacgcttc
    11761 ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta tggaaaatgg
    11821 ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt acgccacatg
    11881 ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca tcggcacaaa
    11941 ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg gctatggctt
    12001 gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc gttgggcagc
    12061 gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc gagccgagtt
    12121 gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg caattgatct
    12181 tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact tcgattttat
    12241 ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac aaaatcgaag
    12301 atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg aatggtggca
    12361 ctataagttg attgatgagc cataccccga tacctatttt aattttgccg tttcataatg
    12421 aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt catggacaat
    12481 cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat agtattcaat
    12541 ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca cgcccatttt
    12601 gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg gcagcaaaag
    12661 gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg ctctaatcca
    12721 cttccaccac tcaaaaaaag ccttgcccct ccatgaactg aaccccaaaa gttgaactat
    12781 ttaatggact gt
//
LOCUS       DASJSZ010000278         7594 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.guided.114, whole genome shotgun sequence.
ACCESSION   DASJSZ010000278 DASJSZ010000000
VERSION     DASJSZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.guided.114"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1215
                     /locus_tag="U4B03_003068"
     CDS             <1..1215
                     /locus_tag="U4B03_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2069820.1"
                     /translation="GLKAYRFSVAWTRIFPNGRGEVNQAGLAFYERLIDELIENDIEP
                     MLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRGKVHYWISLNEQNIFTS
                     LGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMPGKIGPSFAYTPNYPID
                     SNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGWAPTIEAGDAELLESAK
                     PDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVPGMYKKVENPFVERTNW
                     DWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQIHDDYRINYLQSHVKA
                     IKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDETQEGSLERYKKDSYYW
                     YQKLIEENGENL"
     gene            1404..3836
                     /locus_tag="U4B03_003069"
     CDS             1404..3836
                     /locus_tag="U4B03_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN2069821.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            4066..5133
                     /gene="rlmN"
                     /locus_tag="U4B03_003070"
     CDS             4066..5133
                     /gene="rlmN"
                     /locus_tag="U4B03_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN2069822.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5326..6429)
                     /locus_tag="U4B03_003071"
     CDS             complement(5326..6429)
                     /locus_tag="U4B03_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2069823.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            6981..7529
                     /gene="aac(6')"
                     /locus_tag="U4B03_003072"
     CDS             6981..7529
                     /gene="aac(6')"
                     /locus_tag="U4B03_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN2069824.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2542 a         1305 c         1642 g         2105 t
ORIGIN      
        1 ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa tggacgagga
       61 gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt gattgaaaac
      121 gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt gcaagatgaa
      181 tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc agaaacatta
      241 tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca aaatatcttc
      301 acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc gaaacgaatg
      361 tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa atttcacgag
      421 ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta tccaattgac
      481 agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc caattattgg
      541 atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt agaagaaaaa
      601 ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc taaaccagat
      661 tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt aaacggagtt
      721 ggtgctggaa aaatgaatac gacaggaaaa aaaagttcat cagaagaaac tggagttcct
      781 ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga ctgggagatt
      841 gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg catgccggta
      901 ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa acaaatccat
      961 gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg
     1021 gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct tagctggctg
     1081 aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac acaagaagga
     1141 tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat tgaagaaaat
     1201 ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata tggccgtgcc
     1261 ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg aaaaatgaaa
     1321 gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg tataatgaaa
     1381 cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa
     1441 agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg attggtgttg
     1501 ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt ttaggaatat
     1561 tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc tgtcttttat
     1621 tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt ggtaaaaacc
     1681 gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta catgcccatt
     1741 tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg gatttgttag
     1801 ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg attggagcaa
     1861 tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta gcagcagtgc
     1921 tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag ctgttaaatg
     1981 gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct gccaagcaag
     2041 ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa
     2101 ggcgcttagc tgcaaaagaa gctgcagaat atgagaagaa aaaagcggtg agccagcaaa
     2161 gggaaaatac tgctgatgaa tggcaggaac cagaaaatac agagccggag cagctaagtt
     2221 ttgttccaat cgacagtttt caagaaaata tccatcctgc gaatctagag aaaccagtac
     2281 cagatacacc aaaacagacc aatacagcag aaggtttttc agatgagttg ccagaggatg
     2341 acggtacctc attagaattt gagattgaag cagaacagga aaatcaagac tatgaattac
     2401 cgacagttga tttgcttgat tctataccaa ctgtggatca aagtgatgag tataaaaaaa
     2461 ttgaaaaaaa tatcggcgta ttagaacaaa ctttccaaag tttcggtgtc gatgccaagg
     2521 tcgtcaaagc cagcttagga ccatccgtca caaaattcga agtccagccg gctgtagggg
     2581 taaaagtaag taaaattgtg aacttaacag atgatatcgc gcttgcttta gcggcgaaag
     2641 atgtccggat ggaagcaccg attccaggta aatcactgat tggtattgaa gtaccaaatg
     2701 gaaagatcag tatggtttcc tttagagaaa tcatcgaagc acaaccaaac cacccagata
     2761 aattactaga agtaccttta ggacgtgatg tttctggacg ggtacaaacg gctgatcttt
     2821 cgaagatgcc tcacttactc gtggctggtt cgactggtag tgggaagtca gtagcaatta
     2881 atgggattat cacaagtata ttaatgcgtg ctaagcccca tgaagtaaaa ctgatgatga
     2941 tcgatccgaa aatggttgaa ttgaatatgt acaacggcat acctcatctg ttgacgccgg
     3001 tcgtgacgaa ccctagaaaa gcagcgcaag ccttacaaaa agtagtacaa gaaatggaag
     3061 aacgatatga aaaattcgcg gcaaccggag taagaaatat ttctggttac aatgaatttg
     3121 ttcaacagaa aaacttggaa aacggaacga aacatccaac cttaccattc atcgtagtga
     3181 tcgtagatga attagctgat ttgatgatgg tagccagcaa tgaagttgaa gatgcgatta
     3241 ttcgtttagc gcaaatggct cgggcagcgg ggattcatat gatcctagct actcaacgtc
     3301 caagtgtgga tgttatcaca ggaatcatca aagcaaatgt accttccaga atggcatttg
     3361 cggtatcaag cggtacagat tctcggacga tcattgattc taatggggca gaaaagctct
     3421 tagggcgagg agatatgcta ttcttgccaa tgggtgagaa taaaccgatc cgtgtgcaag
     3481 gagcattcat ttctgaccac gaagtagaac gggtcgttca atttgttaca gatcagcaag
     3541 aagctcatta tgaagagaaa atgatgccga cagatgaagt tgaaacgaca ggtgctccag
     3601 aacagccaca agatgagctg tttgaagaag caaaagcact tgtcgttgaa atgcaaacgg
     3661 caagtatttc ccttctccaa agaagatttc ggatcggtta caaccgagca gcacgtttag
     3721 tagatgaact ggaagctcat ggagttgttg gcccgtcaga aggaagcaag cctcggaaag
     3781 tattcattga gcaggaagaa acatcagaaa cgttgccgga cgataatgac atgtaaaact
     3841 tgtgtgatga agggttgaaa aacgaataac caataaaaag acaaggcatg atgataccaa
     3901 aagaaaaaat ggtttttttc catcttttct aatacggtat gatgtcttgt ctttttagtt
     3961 tttgtgagat taaagttcta ttcgatttat ttgtgaagta ttgcattaag gaaatgaatc
     4021 atgtatagta tgtagcgaaa ctttgagaaa aagagggaaa atactttgga aaaacagtcc
     4081 atttatggct tgacgaatga agaattaatc aattggttta tagaaaacgg cgagaaaaaa
     4141 ttccgagctg cccaagtttg ggaatggttg tatcaaaaac gtgtaagtaa tttcacagaa
     4201 atgacgaatc tatcgaaaca attgatcgaa aaattatcgg agcattttat catcaatcct
     4261 ctaaaacaga tggtcgtcca agaagcttcg gatggtacag taaaatattt gtttgaactc
     4321 cctgataaaa acatgattga gactgtactg atgagacaag aatatggctt gtcagtttgt
     4381 gtgacgacac aagtaggatg taatatcggc tgtacgtttt gtgcaagcgg gctgctcaaa
     4441 aagaaccgtg atctgactgc tggagaaatt gttgcacaga tcatgatggt ccaacattat
     4501 tttgatgagc gcaatcttgg cgaacgcgtt tcacatgtcg ttgtaatggg gatcggtgag
     4561 ccatttgata attacgacaa tgtaatggac tttttgcata tcatcaatga tgccaaagga
     4621 ttggcaattg gtgctcggca cattacagtt tccactagtg gattagcaca caaaatcaag
     4681 gaattcgcta ataacggctt gcaagtcaat ctggctattt ccttgcacgc gccgaataat
     4741 gaggtgcgaa catcgatcat gcggatcaac cgaagtttcc caattgagaa attgatggaa
     4801 gctgtggatg agtatttaga gaaaacaaac cgtcgaatca cgtttgaata tatcatgttg
     4861 aatcaagtaa atgatagacc agagcatgcg caacaattag cagatctatt aaaagataag
     4921 aaaaaattag catatgtcaa cttgattcca tataatccag tcagtgaaca tgatcaatat
     4981 tctcgcagtc caaaagctga tgtattgaga ttttatgatg tattgaagaa aaacggcgta
     5041 aactgtgtga tacgtaaaga acatggtaca gatattgatg cagcttgcgg acaattgcga
     5101 agcaagcaaa tgaaaaaaac agttgccaaa taatcgtatc ctactcgttt tttcaattac
     5161 cataaaagga gcagttttct taattaagga actgttcaac ttaaataaga gaaaagattc
     5221 tatagcgaaa gctataaagc tttttttgac aagaaaaacc ctcatcaagc aaagaaatgt
     5281 cctcaatttg atgacagcct gcttgatggg ggttttagca attatttaac tgattttctt
     5341 atatccttgc tggctttctt gtgtagcatg ataagtttct acgtcaagaa tggcctgttt
     5401 aaatttttct cgggtgattt cataaggcac atgtttaata tcatttgttg ccatggcttt
     5461 atctaagatt gcttctaaat cggtcgtgac attgagattc atgtcggtta acgatactgg
     5521 aagttggttc tttttcataa acacatagat tttatcaaac aaatcgaact gctgatccat
     5581 catcaataag gtcagtacac cataactgac agatgctccg tgaaggtatc cctccatttt
     5641 ttctagaaca gtcgtaccgt aatagaatga gtgtgccata gcagaattat agttattgtt
     5701 aactaaaaca gaaaccaatc cggtagtaac aatgatatcc gaaacgactt gttcaattgc
     5761 atatgaggga cgatcgtcgg cactatcttt caatgcttgc gtaccataag tcaagagacg
     5821 atcaatgcac atttttccga tttggattcc taatctatcc gtgtagccta gatcatcgcc
     5881 acgagcagaa aaagtagatt cgacttcttt ggataaagca tcgccaatac ctgcccataa
     5941 gtatttatca ggtgcttcgg caataattgc tgtatcaatg aaggtgtgta caggtggttt
     6001 tgaaggaaag aagagtccgt ccattacgcc ttcttcgtta tagatgacac ataccgcagt
     6061 gacgggcgaa cagttcgaac caatagtggg aaaggtgaag actggtttag ctagtttttc
     6121 accaatgact tttacagtat cgcaagcacg gccaccaccg actgcaaaca ggacgtctgc
     6181 tttttggaca ctttcatgct ggatcaactg ttcaatattt ccataagttg aattaccccc
     6241 gtaccagaga ctggcgacaa cctctatttc ctgtgtcaaa gctactttta ttttgttttc
     6301 tgcctttgaa agtgcagtct tccctccaat cagcactact tttgtcccat attctttgac
     6361 gattccattt gctttttcat acgcatctgt tccaatggta taatttggta agaaaacact
     6421 ttgactcata ttcatttcct tctttcttcg ttgaaacggg ttaaaatttt aaagagtgat
     6481 cgatctgact gcggctgtag atggagtaga ggtgaaccta agacaggata agtgatgaat
     6541 aaggtggcat gaacaccttg aaatcatcaa taagtatcct aaaaacgaac aagtacaaaa
     6601 acattgaaaa cgacaataat gaatttttgt tcctcttagt atttctaccg attccctttg
     6661 cttttttgta attatctaaa aattctaaaa attaagatta ttatatgaaa acaggtttta
     6721 tttgtcaatt cataattggt tttttttaaa gcaaaagata atctatatca tgatgaacaa
     6781 acagagtttt ttcctccaat tgatggcttt cctgatcttt cctattttga ataaagtgtt
     6841 tctgtataat tttttatttt ataagtaaga taaataatga aagaatagag atactgattg
     6901 gtagctaaag atttttgagc gagaacattt cgttttttca aaaggactgt tagaataagc
     6961 aagaacaagg gggaagacgt atgataatca gtgaatttga ccgtaataat ccagtattga
     7021 aagatcagct ttctgattta ctgagactga cttggccgga agaatatgga gacagctcgg
     7081 cagaagaagt agaagaaatg atgaatccag aacgaatcgc ggtagcagcg gtagaccaag
     7141 atgagttagt aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc
     7201 atccattagt tgtagaaagc tcccgacgaa agaaccaaat aggtactcga ttagtcaatt
     7261 acttagaaaa agaagtagct tccagaggag gaatcacgat ttatttaggt acggatgatt
     7321 tagaccatgg aacaacgtta agtcaaacgg acctgtatga gcatacattt gataaagtgg
     7381 cttccatcca gaaccttcgt gaacatccgt atgaattcta tgaaaaatta ggttataaaa
     7441 tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc ggatatttgg atggcaaaaa
     7501 cgattattcc tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa gtaatcagct
     7561 tcaagaaata agaaggaatt tacgaaaatt actt
//
LOCUS       DASJSZ010000279         7466 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.guided.115, whole genome shotgun sequence.
ACCESSION   DASJSZ010000279 DASJSZ010000000
VERSION     DASJSZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.guided.115"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(91..1431)
                     /locus_tag="U4B03_003073"
     CDS             complement(91..1431)
                     /locus_tag="U4B03_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2069825.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1700..2596
                     /locus_tag="U4B03_003074"
     CDS             1700..2596
                     /locus_tag="U4B03_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN2069826.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(2746..3687)
                     /locus_tag="U4B03_003075"
     CDS             complement(2746..3687)
                     /locus_tag="U4B03_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN2069827.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            3813..4934
                     /locus_tag="U4B03_003076"
     CDS             3813..4934
                     /locus_tag="U4B03_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2069828.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5192..6694
                     /gene="eat(A)"
                     /locus_tag="U4B03_003077"
     CDS             5192..6694
                     /gene="eat(A)"
                     /locus_tag="U4B03_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN2069829.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            6722..7288
                     /locus_tag="U4B03_003078"
     CDS             6722..7288
                     /locus_tag="U4B03_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2069830.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2516 a         1362 c         1493 g         2095 t
ORIGIN      
        1 aaaaggagtt gcaacaagac ttttgtcaca actcctttct tcaatagctc catctattcc
       61 acataaataa aataaaattt tctatcgatt tcattcattc gtcaatacga acgcatcatt
      121 ctttttttga taactgaaat aaatgaacag gtaaagcagg ccagtcaata cagttacccc
      181 aatggagttc gtgaacagga taccaaggaa aatcaatcct aagacagcta atgcaaaaaa
      241 gctgattcct ttatcccata atgttccttt tcttagtgcc agtacaaagg atgccgcact
      301 gataatgatc cagatcaata atactgtata cgaaagggaa cctgccagat agccaaataa
      361 tttgtcaccg acaaaataag aaagagctac accaagataa agaacactgg tacaaaataa
      421 aacagcacgt tgcggtactt gatgcttgtt taaaacagct agttttctag aggaaccttt
      481 tcgatccttc aaacggaaga acaaaattct tgaagatgca tacacacccg aattgataga
      541 tgagaacaaa gcaaggacaa tcacaaaatt gacgatatcc gctgcaaatg ggatattcat
      601 tttttcaaat accatcacaa aaggactgac attcgtgcct gctaaagtat tccaaggaaa
      661 aagaatcaaa agcaaaaaca aaggaatgat gtaaaaggaa atgattcttc ccatgacacc
      721 tttgattgct ttaggaatcg cacgtttagg atcatctgct tcgcttaccg tgatagcaat
      781 cagttcagaa ccgccgtaag aatagataac tacaagcaaa gaactgatga ttcccttcat
      841 accatgagga gtaaacccac catggtcagt caattgctga agggtaggga aaacgcctgt
      901 gcccaacatt tgctgaccga caagataagt agcaaaaatg atcagtaaga taatgacact
      961 gattttagca aaagccagcc aatattctgt ttctgcgaat aaccgtaccg aataaagatt
     1021 gatgatcgta gttaaaacag ccaacataaa aacaaatacc caggctggaa tgtgcgggaa
     1081 ccacaactgc aaaaaggaag cagctgcaac agcttcagcg atgatattga taatccacat
     1141 agaccagtag acccaatcag taaaatcagc agaatgagca cctagataag ggcgtatcaa
     1201 accagataat ccgtgatgcg tgtcgccacc gcttaaaacg atttttccca gacctttcat
     1261 cacgatatat aaaatcagcc caccaatcaa aaaagcaatc aatacagaag ggccggcgat
     1321 cccaatcgct tcgccacttc ctttaaatag acctgcaccg attgctccac ctaaagctaa
     1381 catggtgata tgtctggagc taagttgttt tttcaatttt ttatcttcca taaatgacct
     1441 ctttctttta aattcacaat caaaatttat attaagaata aatagcaata tttaacaatg
     1501 actaatgata acaaattttc agacaattac aactattcag aaaactttta ggcgaatttt
     1561 aatagaagaa tgatttattt catcatattt tcatacaaat gttaaatttt catacaaata
     1621 taggaaacgt tttactggct ttttactatt taaacaggct atcataggaa aaagtgacag
     1681 agaaagtgag gcggaaaaga tgaacaaaaa tgacgtactg ctagttgcaa aaaataaatc
     1741 actttatttt ttgccttcca tccgctcttt agatgtttgg tccggaaaag atttactaca
     1801 gtcggaattc attaaatcag ctagacaaca gttggcaaat tatcagctga tcgttttttt
     1861 agattatgga tttgatatgg agatggctcg acgcgtgcgc ccatttacca atgcaaaaat
     1921 tgtgttattt ttttggaatc acttcaaagc agaacacgag gcgatgctga agcaggcaca
     1981 agatgaaccg gcaatcgatg agatttatca ttttgatttt ttggaagcaa aagaacttgg
     2041 gctgaaacat aatagttcat tttattcgaa acatatgaat ttttcttcta gcgaaccaaa
     2101 gacggatcta ttttttggtg caacgaataa tgggagaaaa gaacgagcag aaagctataa
     2161 gcaggaattt ataaaaagag atgttacgac caactatttc attcttccac atcgagggaa
     2221 tgaacaacct ggatatctta cttatgaaga gtacctgaaa aaaacagggg aatcgcgcgg
     2281 gattctggaa ctgttgcggg agggtcagca aggagtgact ttgcggacat ttgaatccat
     2341 gtattttcaa aagaaactag tgacggataa tcaagcgatt gctcattatc atttttatcg
     2401 tcctgaaaat atttttttgc ttcgagaacg tgatttagat gaattaccgg aatttctgca
     2461 tacaccattt aagccaatcc tatcagagct attggaattt tatgatgcag aaaattgggc
     2521 aaaacgattt atcactaacg attccactat ttatgagcaa tacgaatatg acctctcctc
     2581 tgttgaaaac aaataaaaaa cctacatttt gaagagatta tgtactgaaa caaacaagca
     2641 caattcttat ggcatcatga gagttgtgtt ttctttttga tcttttattg ggcttaaaaa
     2701 aagagaacca gctgctgatt cagctgattc tcatcagaga gccttttatt ctgcgccaag
     2761 gattccttct tttgcatgga ctaattgggt caaaaaggca caatatggtg tagcaatatc
     2821 atatttttgt ccttttcgcc aaatggcacc attgatgtag tcgatttcag tcaaacgatg
     2881 gtttttgatc aggtcttggt acatagatgg gtaatgcaac ccgatgttgt caggatcata
     2941 agtagaagca atatgttggt acacttcttc ttgatctagt atgattcctt cttttgctgc
     3001 gatagaagca aattcagata cgattgttcg aaccatgctc tctgccgctt tttgcgcgcc
     3061 tagttcagca atattacaat ccaaaatcgt gcataaacca ttcaatgttc catttacaca
     3121 agcttttcgc caaatggaat aacgaacgtt atggctatag atcggattta aatttgcttc
     3181 ttgaaagact tctacaactt tttttgtaaa tgcttcgcct tctggttcca agttctctag
     3241 ttcgatttct ccatcaccta acaatgtgac ttttccggga ccagctagac cagctgtcca
     3301 catcgtgatc ccaaataaga tatttttctt tggcacatat ttttccagta catcttcatg
     3361 acctaatcca tttagtaaac acaaaacata agtattgtca gtaataatgg attggatcga
     3421 acaaaacata tcatctaatt gattggcttt tgttaatgct acgatcaagt ccacgtgttc
     3481 gttgctttcg atgatttctt ctggtgaata gattggcagt tttgcgacca cttctttacc
     3541 gttaaagtca gcaatcaacc catttgtacg aatggcttca atgtgagcag gccaacgatc
     3601 gatcaagagg acctcatttc cgctttgatg tagcatcaat ccgattcggc tgcccattgc
     3661 tccggcccca gctattgcga ttttcatgtt ttttctctcc tttattcaat gagttccctt
     3721 ttagtatata gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag
     3781 tgtcttactt aagaagaaag tgagggatac agatggatat tttggcggca attgattcat
     3841 ttaaaggaag tgcttcttca tttgaactaa atcaggcagt tctaaatggc tgggaagatc
     3901 gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa
     3961 tctattacgc gctcagggga acatggcgta ccattgatgg gatggatctc ttattccgtc
     4021 agcgaaaaat cagctattta ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat
     4081 ctgcacaagc attggggtta gatctacttg ataaaccaac ggatcaaact atccgacttg
     4141 cttccagtta tgccttagga gaacttatta aagatgcact gcttcaagaa gtagaacaaa
     4201 ttattgtcac tttaggagga agcgggtgta gcgatggtgg tctaggatta ctccaatcct
     4261 tgggtgcttc tttagaaggg tacacagaag gaaatccttt attatctact aaaaaacttc
     4321 attttgatcg aagaaacgtt cttgtttcta aagtaaaatt gattatcgct gcagatgtta
     4381 ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa
     4441 tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag
     4501 atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg
     4561 gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca
     4621 ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg
     4681 atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc
     4741 gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt
     4801 ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac
     4861 acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca
     4921 tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga
     4981 caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact
     5041 gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga
     5101 tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata
     5161 ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac
     5221 attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac
     5281 acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat
     5341 tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt
     5401 cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga
     5461 ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt
     5521 atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt
     5581 atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc
     5641 tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag
     5701 ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca
     5761 actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga
     5821 atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc
     5881 agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg
     5941 attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg
     6001 gacgatgaaa cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga
     6061 aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca
     6121 ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga
     6181 gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc
     6241 aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga
     6301 aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg
     6361 aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa
     6421 cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat
     6481 gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac
     6541 atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat
     6601 cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt
     6661 atctactgag attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg
     6721 tatggttaag gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca
     6781 cggagaaaca acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac
     6841 tgaaaaagga cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc
     6901 aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc
     6961 tgatcgtctc agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga
     7021 ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt
     7081 acgattccca aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa
     7141 agaatgcatc gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag
     7201 agtgatcaat gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga
     7261 taatacagaa ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt
     7321 caaggcactg cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata
     7381 taaactcaac ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt
     7441 ctttagatat atactctagt cataga
//
LOCUS       DASJSZ010000280         3796 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.guided.157, whole genome shotgun sequence.
ACCESSION   DASJSZ010000280 DASJSZ010000000
VERSION     DASJSZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.guided.157"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            110..193
                     /locus_tag="U4B03_003079"
     CDS             110..193
                     /locus_tag="U4B03_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEN2069831.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="U4B03_003080"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="U4B03_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN2069832.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="U4B03_003081"
     CDS             complement(1318..2181)
                     /locus_tag="U4B03_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2069833.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="U4B03_003082"
     CDS             complement(2183..2455)
                     /locus_tag="U4B03_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2069834.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="U4B03_003083"
     CDS             complement(2472..2681)
                     /locus_tag="U4B03_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN2069835.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="U4B03_003084"
     CDS             complement(2759..3655)
                     /locus_tag="U4B03_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2069836.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1374 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccctt
//
LOCUS       DASJSZ010000281         1369 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.guided.216, whole genome shotgun sequence.
ACCESSION   DASJSZ010000281 DASJSZ010000000
VERSION     DASJSZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.guided.216"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(699..989)
                     /locus_tag="U4B03_003085"
     CDS             complement(699..989)
                     /locus_tag="U4B03_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2069837.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          444 a          249 c          208 g          468 t
ORIGIN      
        1 tatatttcct aatagagtat aacatagctt taagcattac ttaaagataa cttaaaagtt
       61 atcttttaaa tacttaattg ttaaatacct tatttgatgc aaaagaaatc tacgatagtc
      121 aacaaaaaaa ttttcattat atccagaaaa atttttctta gttaaggacc tatggattgc
      181 agtaatgcag aattcgtcga ataaactgcc aaagacatac aatcatattg cttttaaaat
      241 tgacgaacaa gatatagatt cgtttgtctc aaaaatccaa atgttaggtt taactgtcga
      301 accaggacgt tctcgtgtga aaggcgaggc ttcttcaatt tacttttacg actatgataa
      361 tcacctcttc gagttacata ccggtactct ccaggaacgt ttaaaatcct acaattccac
      421 tacctgagac tagtaatgac tagtctcttt ttatggcctc tgatcccccc attgagcgaa
      481 tactaatagg gcttcgaatt gatctgttca atctttaagc aatatccctc aaatgagttt
      541 ggaaagttta gtgattcatc acaaatactt aaatacatgt agttatatat agattgaggt
      601 tcttgtgagc gtatgctcgt gtgggttcaa atcccatcgt ctgcatccat ctttccaaaa
      661 caagcggcta ggacattttt gtctcagccg cttgttttct agtttattct ttcatcttgt
      721 cggacctttt ttcttgggta tagacttttc tcatattcta ccacaatttt gggtttccct
      781 aacacatgat aggatctcac aacaaattgc tttctattat tataataacc tgtaacgact
      841 aacttcatcc cttcgtctac ttcagctagg aagttcaaac tatgggaagc aatcaaacaa
      901 ttgaccccat ttaaactgaa acggatcaac ggtctggcac taacttgcaa agtacggatc
      961 ttatctactg ttccatattt tttctccata accttcacct caataccatt atacgaacat
     1021 acattccctt tgtaaagaag aaactctgtt ttatcttttt gactttttgt ctattacctt
     1081 tcttaaaatc atattggcac ttcctgcgaa gatgaaggac atgtactaaa aaaaagaggc
     1141 agcagtatag atttaagtag ttcagttttg gagtacaaaa agtgaaaatg taaacctaaa
     1201 tgagtatcaa attgcaactg aatttgatac tcgtttttta ttgttaaatc aacgtttgtt
     1261 gttaatttac accacaaaat gtaagttaaa atgtatataa aaatgcgaac gtgaaataat
     1321 tattgtagga atgcaaattg gtgtgttaaa atggttacaa tttactaag
//
LOCUS       DASJSZ010000282         1162 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5681
            SAMN38756300-rid20572223.guided.229, whole genome shotgun sequence.
ACCESSION   DASJSZ010000282 DASJSZ010000000
VERSION     DASJSZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756300
            Sequence Read Archive: SRR27167978
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028142.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028142.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 02:49:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,025
            Genes (coding)                    :: 2,957
            CDSs (with protein)               :: 2,957
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 29 of 68
            Pseudo Genes (incomplete)         :: 25 of 68
            Pseudo Genes (internal stop)      :: 20 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756300-rid20572223.guided.229"
                     /isolate="VRE5681"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-11-02"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            286..783
                     /gene="dfrG"
                     /locus_tag="U4B03_003086"
     CDS             286..783
                     /gene="dfrG"
                     /locus_tag="U4B03_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN2069838.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(855..>1162)
                     /locus_tag="U4B03_003087"
     CDS             complement(855..>1162)
                     /locus_tag="U4B03_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN2069839.1"
                     /translation="PNEIAKMLLNVNDHSTNSFIQQIRRYISSLERPLTTARGDKKSY
                     IYANFNPKYAQFAITILRTYYNFCRPFKSADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          421 a          137 c          222 g          382 t
ORIGIN      
        1 gtaatctata ctaagtagtt cagttttgga gtacaaaatg acaaaatgta cttcaaaatg
       61 gactactttt ttcattttaa cttacatacc aaattacata caaaatgatt tataaaatgc
      121 aattgaaaat gataattgaa tgaaaatttg tgattttgtg gtattatgat aatataactt
      181 atagaaaaaa ttgcttattc aactaacggg caggataatg agaaaaggtt ttttgataga
      241 aataagaaat tagatggtaa attagttaaa aaggctgtgt tataaatgaa agtttctttg
      301 attgctgcga tggataagaa tagagtgatt ggcaaagaga atgacattcc ttggaggatt
      361 cccaaggact gggaatatgt taaaaatact acaaagggac atccgataat attaggtagg
      421 aagaaccttg aatcaatcgg aagagcctta cctgacagaa gaaatattat tctgacgaga
      481 gataaggggt ttacctttaa tggttgtgaa attgttcatt caatagaaga tgtttttgag
      541 ttatgtaaaa acgaagaaga aatttttatt ttcggaggag aacagattta taatttgttt
      601 ttcccttatg ttgagaaaat gtacatcaca aaaatacatc atgaattcga aggagatact
      661 ttttttccag aagtgaatta tgaggaatgg aatgaggtat ttgcccaaaa agggataaag
      721 aatgataaaa atccgtataa ctactatttt catgtatatg aaagaaaaaa cttattgagt
      781 taacgggtgc gttaattgaa gaagaacagt aaagataaag cacaagagct tgggattact
      841 ccaagctctt tttgtcattt gaaataaatt atatctttcc aatcaaattg tttatctgta
      901 atccctaatc tttgagcagg tgtcaaaacc tttttatccg ctgatttaaa agggcgacaa
      961 aagttgtagt aagttcttag aattgtaatc gcaaattgag catacttcgg attgaagtta
     1021 gcgtaaatat agctcttctt atcaccacga gctgttgtta atggtctttc aagagatgat
     1081 atataacgtc ttatctgttg aataaagcta ttggtcgaat ggtcattaac atttaatagc
     1141 atctttgcga tttcgtttgg tt
//
